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<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YBT2</accession>
    <name>A0A9Q0YBT2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_45176</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010002028">
        <property type="protein sequence ID" value="KAJ8017429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YBT2"/>
    <dbReference type="OrthoDB" id="10053555at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31025:SF29">
        <property type="entry name" value="SI:CH211-196P9.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31025">
        <property type="entry name" value="SI:CH211-196P9.1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8017429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="294" mass="33907" checksum="0BCC68DA5509CEE9" modified="2023-09-13" version="1">MDYLSDHPQGKELIDRYRTSAQKEQFARDIVKTFPILKDTTPGMEGHIHEMKYTPPTKVNKSRILSLFKETHHHRRNWLMQKTVDITTFLQEYPRMQDMPELVNVEFQQLYPDNHSAFLRKWESFYVPRILQIALEEYPSITSTVKSFDGGDQNLCAAVVLAHMLFRTSKKVSSRADAIKYLYQTVPLGTDLADEARRKDVCYKQPFLLAVGPLKQPTQYVMVVDQLSIPAGDSPVTAIDALFKAYYCFNVEFPISVSQFWEFLAFEVNEVMDKKEAKPQVRSLASIIRGLAAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YC34</accession>
    <name>A0A9Q0YC34_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_41704</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8019918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YC34"/>
    <dbReference type="OrthoDB" id="203440at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8019918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="103" mass="12147" checksum="B7FBD7FBA27BA6CD" modified="2023-09-13" version="1">MFLDSALDIHELTNTINSYTGFCEDLVIPQRPVKCYPNKPWITKEIQYLLSRKKHLFKSGTKQELKIIQSEINTAIKREDVYRRKIENNFMTNNIRSVWDGLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YDD0</accession>
    <name>A0A9Q0YDD0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Receptor-interacting serine/threonine-protein kinase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_42074</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000027">
        <property type="protein sequence ID" value="KAJ8019380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YDD0"/>
    <dbReference type="OrthoDB" id="100767at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01670">
        <property type="entry name" value="Death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000488">
        <property type="entry name" value="Death_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016729">
        <property type="entry name" value="FADD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15077:SF12">
        <property type="entry name" value="DEATH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15077">
        <property type="entry name" value="FAS-ASSOCIATING DEATH DOMAIN-CONTAINING PROTEIN FADD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00531">
        <property type="entry name" value="Death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00005">
        <property type="entry name" value="DEATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50017">
        <property type="entry name" value="DEATH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0675">Receptor</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Death" evidence="1">
        <location>
            <begin position="5"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8019380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="95" mass="11293" checksum="DAE67EDFF15E2399" modified="2023-09-13" version="1">MEIVKDKDLLDLSKRIAAEWEFVARYLTIEEAQINIFKKDYDHTRERIYQMLLFWKKEKGNGATYRVLTQALRQSERNDLADDLSLRQGNMGIIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YEN4</accession>
    <name>A0A9Q0YEN4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Cadherin EGF LAG seven-pass G-type receptor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_40760</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8021010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YEN4"/>
    <dbReference type="OrthoDB" id="6146532at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13952">
        <property type="entry name" value="7tm_classB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053231">
        <property type="entry name" value="GPCR_LN-TM7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001212">
        <property type="entry name" value="Somatomedin_B_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45902">
        <property type="entry name" value="LATROPHILIN RECEPTOR-LIKE PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45902:SF1">
        <property type="entry name" value="LATROPHILIN RECEPTOR-LIKE PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50958">
        <property type="entry name" value="SMB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="589"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="624"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="655"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="698"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="746"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="domain" description="SMB" evidence="8">
        <location>
            <begin position="50"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="7">
        <location>
            <begin position="590"/>
            <end position="765"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8021010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="839" mass="94461" checksum="B6F8819A7F2DAE1D" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YF43</accession>
    <name>A0A9Q0YF43_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">tRNA (guanine(10)-N(2))-methyltransferase TRMT11</fullName>
            <ecNumber evidence="12">2.1.1.214</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">tRNA methyltransferase 11 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HOLleu_39905</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Nanhai2018</strain>
            <tissue evidence="18">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalytic subunit of the TRMT11-TRM112 methyltransferase complex, that specifically mediates the S-adenosyl-L-methionine-dependent N(2)-methylation of guanosine nucleotide at position 10 (m2G10) in tRNAs. This is one of the major tRNA (guanine-N(2))-methyltransferases.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>guanosine(10) in tRNA + S-adenosyl-L-methionine = N(2)-methylguanosine(10) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43128"/>
            <dbReference type="Rhea" id="RHEA-COMP:10355"/>
            <dbReference type="Rhea" id="RHEA-COMP:10357"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74481"/>
            <dbReference type="EC" id="2.1.1.214"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:43129"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Part of the heterodimeric TRMT11-TRM112 methyltransferase complex; this complex forms an active tRNA methyltransferase, where TRMT112 acts as an activator of the catalytic subunit TRMT11.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM11 methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.214" evidence="12"/>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YF43"/>
    <dbReference type="OrthoDB" id="296065at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043527">
        <property type="term" value="C:tRNA methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160102">
        <property type="term" value="F:tRNA (guanine(10)-N2)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000241">
        <property type="entry name" value="RlmKL-like_Mtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016691">
        <property type="entry name" value="TRMT11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059073">
        <property type="entry name" value="TRMT11_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13370">
        <property type="entry name" value="RNA METHYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13370:SF3">
        <property type="entry name" value="TRNA (GUANINE(10)-N2)-METHYLTRANSFERASE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25904">
        <property type="entry name" value="Tmrp11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01170">
        <property type="entry name" value="UPF0020"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017259">
        <property type="entry name" value="tRNA_mtfrase_TRM11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00507">
        <property type="entry name" value="N12N6MTFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51627">
        <property type="entry name" value="SAM_MT_TRM11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 15" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 15" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 15" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 15" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="3 15" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="tRNA (guanine(10)-N(2))-methyltransferase TRMT11 N-terminal" evidence="17">
        <location>
            <begin position="1"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosomal RNA large subunit methyltransferase K/L-like methyltransferase" evidence="16">
        <location>
            <begin position="141"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00050985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00056270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00065434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00066937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00067484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00075308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF25904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ8020336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="424" mass="48770" checksum="9E66A7C9450C7B88" modified="2023-09-13" version="1">MVKFPSEESVHTYMTRSILGRTVMEVLAHGTSREEACQQLKNLPECQTYPYCQEDSSFCWRVHSFGKKIKQEKRVDIIHQFIVNLPLKGPVNLETPDNLFYICEDYGPDPNSAPTVPYYVYVGRKIAEGQRHLAHQYSVKTRHFIGNTSMDAQLSLIMANQGHVKRNMFVFDPFVGTGSLLVAAAHFGAYVAGCDISHNIIHGRGKSSRSTGKSRGQVLYRGNDENIRANMRQYRLQDYYIDVMVSDFALNVWKEKPLFDAIITDPPYGIREKTRKIGTEKELSFLFLFFRLEGHIPSTKSYFLADILKDLLNFSARYLRLNGRLVYWLPVVNDDYSEDNIPRHPCLKLISNSEQRFNKIISRRLITMEKTRSIEDSTVMSEAEIRTDSYEGANAIRKKYFPNNSKTAETLQSELVRKSVDGNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YG33</accession>
    <name>A0A9Q0YG33_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_39365</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8022003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YG33"/>
    <dbReference type="OrthoDB" id="608866at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042162">
        <property type="term" value="F:telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031848">
        <property type="term" value="P:protection from non-homologous end joining at telomere"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11660">
        <property type="entry name" value="SANT_TRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.210">
        <property type="entry name" value="Telomere repeat-binding factor, dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036507">
        <property type="entry name" value="Telomere_rpt-bd_fac_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030657">
        <property type="entry name" value="TERF2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46833:SF1">
        <property type="entry name" value="TELOMERIC REPEAT-BINDING FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46833">
        <property type="entry name" value="TELOMERIC REPEAT-BINDING FACTOR 2 TERF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63600">
        <property type="entry name" value="Telomeric repeat binding factor (TRF) dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH myb-type" evidence="3">
        <location>
            <begin position="617"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="617"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="215"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="281"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="355"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="462"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="294"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="359"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="371"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="390"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="464"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8022003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="678" mass="77149" checksum="F7C724899AE00849" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YH95</accession>
    <name>A0A9Q0YH95_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">DNA ligase</fullName>
            <ecNumber evidence="18">6.5.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">HOLleu_39441</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="24">Nanhai2018</strain>
            <tissue evidence="24">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17 18">
            <text>ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + diphosphate.</text>
            <dbReference type="EC" id="6.5.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 19">Belongs to the ATP-dependent DNA ligase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.1" evidence="18"/>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8022059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YH95"/>
    <dbReference type="OrthoDB" id="206088at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070421">
        <property type="term" value="C:DNA ligase III-XRCC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003910">
        <property type="term" value="F:DNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071897">
        <property type="term" value="P:DNA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006273">
        <property type="term" value="P:lagging strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07902">
        <property type="entry name" value="Adenylation_DNA_ligase_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07967">
        <property type="entry name" value="OBF_DNA_ligase_III"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.30:FF:000003">
        <property type="entry name" value="DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3260.10">
        <property type="entry name" value="DNA ligase, ATP-dependent, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.30">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1740.10">
        <property type="entry name" value="Zinc finger, PARP-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019406">
        <property type="entry name" value="APLF_PBZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050191">
        <property type="entry name" value="ATP-dep_DNA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000977">
        <property type="entry name" value="DNA_ligase_ATP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012309">
        <property type="entry name" value="DNA_ligase_ATP-dep_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012310">
        <property type="entry name" value="DNA_ligase_ATP-dep_cent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016059">
        <property type="entry name" value="DNA_ligase_ATP-dep_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012308">
        <property type="entry name" value="DNA_ligase_ATP-dep_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036599">
        <property type="entry name" value="DNA_ligase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031916">
        <property type="entry name" value="LIG3_BRCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001510">
        <property type="entry name" value="Znf_PARP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036957">
        <property type="entry name" value="Znf_PARP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00574">
        <property type="entry name" value="dnl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45674">
        <property type="entry name" value="DNA LIGASE 1/3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45674:SF9">
        <property type="entry name" value="DNA LIGASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04679">
        <property type="entry name" value="DNA_ligase_A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01068">
        <property type="entry name" value="DNA_ligase_A_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04675">
        <property type="entry name" value="DNA_ligase_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16759">
        <property type="entry name" value="LIG3_BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10283">
        <property type="entry name" value="zf-CCHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00645">
        <property type="entry name" value="zf-PARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01336">
        <property type="entry name" value="zf-PARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56091">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00697">
        <property type="entry name" value="DNA_LIGASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00333">
        <property type="entry name" value="DNA_LIGASE_A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50160">
        <property type="entry name" value="DNA_LIGASE_A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50064">
        <property type="entry name" value="ZF_PARP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="9 18" id="KW-0227">DNA damage</keyword>
    <keyword evidence="13 18" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="14 18" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4 18 24" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PARP-type" evidence="21">
        <location>
            <begin position="90"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-dependent DNA ligase family profile" evidence="22">
        <location>
            <begin position="518"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="23">
        <location>
            <begin position="852"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="184"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="208"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="745"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="20">
        <location>
            <begin position="186"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="208"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="774"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="804"/>
            <end position="813"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00034003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="KAJ8022059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="933" mass="104324" checksum="0479CA0E2CA0665A" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YHQ2</accession>
    <name>A0A9Q0YHQ2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sulfotransferase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_40436</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YHQ2"/>
    <dbReference type="OrthoDB" id="2405944at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48312">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48312:SF1">
        <property type="entry name" value="SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8020759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="314" mass="36494" checksum="4E28FAED2569AE41" modified="2023-09-13" version="1">MADSSQKQQRIFLWCWPRSISTAVEKCLSFVDGMQTWHEPYTVAFNAELLTNKAIMQEGNSDSPIAKVLQEYNDMMKTLANQPTEFSGGKFMPVANFTYPFVKEELEKEEPGKKYILIKDMATAVLDRLDALPDVPTRHTFIIRHPYRFLLSQRKMFFRISNYQGDPNEFDMFKALPVMSDTIFQRDGMHLVWKHVQESGKDPNPIIIDAEDVMNYPETILPKYLAELGIPFDKKYLTWDESIDSIRKTWKGALEQVALGYRMGVFDKAFNSSCFNPSAHVMPKREELTPDILRIVDFVLPGYEEMYEKRIKPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YHS7</accession>
    <name>A0A9Q0YHS7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Matrix metalloproteinase-16</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HOLleu_37254</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Nanhai2018</strain>
            <tissue evidence="17">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="11">Can bind about 5 Ca(2+) ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M10A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YHS7"/>
    <dbReference type="OrthoDB" id="406838at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0030574">
        <property type="term" value="P:collagen catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0030198">
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="CDD" id="cd00094">
        <property type="entry name" value="HX"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ZnMc_MMP"/>
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        <property type="entry name" value="Matrix metalloproteinase 1, isoform C"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="stromelysin-3 isoform X2"/>
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        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
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        <property type="entry name" value="Hemopexin-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR018487">
        <property type="entry name" value="Hemopexin-like_repeat"/>
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        <property type="entry name" value="M10A_MMP"/>
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        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
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        <property type="entry name" value="Pept_M10_metallopeptidase"/>
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        <property type="entry name" value="Pept_M10A"/>
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    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
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    <dbReference type="InterPro" id="IPR002477">
        <property type="entry name" value="Peptidoglycan-bd-like"/>
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        <property type="entry name" value="PGBD-like_sf"/>
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    <dbReference type="PANTHER" id="PTHR10201">
        <property type="entry name" value="MATRIX METALLOPROTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201:SF294">
        <property type="entry name" value="MATRIX METALLOPROTEINASE 16"/>
        <property type="match status" value="1"/>
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    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001191">
        <property type="entry name" value="Peptidase_M10A_matrix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00138">
        <property type="entry name" value="MATRIXIN"/>
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        <property type="entry name" value="HX"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS51642">
        <property type="entry name" value="HEMOPEXIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="15" id="KW-0732">Signal</keyword>
    <keyword evidence="14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040204217" evidence="15">
        <location>
            <begin position="18"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="508"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase metallopeptidase" evidence="16">
        <location>
            <begin position="104"/>
            <end position="270"/>
        </location>
    </feature>
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        <location>
            <begin position="306"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="13">
        <location>
            <begin position="352"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="13">
        <location>
            <begin position="398"/>
            <end position="445"/>
        </location>
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    <feature type="repeat" description="Hemopexin" evidence="13">
        <location>
            <begin position="446"/>
            <end position="493"/>
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        <location>
            <position position="226"/>
        </location>
    </feature>
    <feature type="binding site" description="in inhibited form" evidence="11">
        <location>
            <position position="85"/>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
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            <position position="123"/>
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        <ligand>
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            <label>1</label>
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        <ligand>
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        </ligand>
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        </ligand>
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        </ligand>
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        </ligand>
    </feature>
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        <location>
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        <ligand>
            <name>Zn(2+)</name>
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        </ligand>
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            <name>Zn(2+)</name>
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        </ligand>
    </feature>
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        </ligand>
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            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="312"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>5</label>
        </ligand>
    </feature>
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        <location>
            <position position="356"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
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        </ligand>
    </feature>
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        <location>
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        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>5</label>
        </ligand>
    </feature>
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        <location>
            <position position="450"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>4</label>
        </ligand>
    </feature>
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        <location>
            <position position="387"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00010370"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR621190-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ8022374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="531" mass="57974" checksum="B9730BA5712CF51C" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YHS8</accession>
    <name>A0A9Q0YHS8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="17">ADAM 17-like protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HOLleu_40053</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Nanhai2018</strain>
            <tissue evidence="17">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YHS8"/>
    <dbReference type="OrthoDB" id="2131567at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006509">
        <property type="term" value="P:membrane protein ectodomain proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04270">
        <property type="entry name" value="ZnMc_TACE_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.70.10:FF:000003">
        <property type="entry name" value="Disintegrin and metalloproteinase domain-containing protein 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.70.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.70.10">
        <property type="entry name" value="Disintegrin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034025">
        <property type="entry name" value="ADAM10_ADAM17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051489">
        <property type="entry name" value="ADAM_Metalloproteinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001762">
        <property type="entry name" value="Disintegrin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036436">
        <property type="entry name" value="Disintegrin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001590">
        <property type="entry name" value="Peptidase_M12B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45702">
        <property type="entry name" value="ADAM10/ADAM17 METALLOPEPTIDASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45702:SF6">
        <property type="entry name" value="DISINTEGRIN AND METALLOPROTEINASE DOMAIN-CONTAINING PROTEIN 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00200">
        <property type="entry name" value="Disintegrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13574">
        <property type="entry name" value="Reprolysin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00050">
        <property type="entry name" value="DISIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57552">
        <property type="entry name" value="Blood coagulation inhibitor (disintegrin)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50215">
        <property type="entry name" value="ADAM_MEPRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50214">
        <property type="entry name" value="DISINTEGRIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3 17" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8 13" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="597"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M12B" evidence="16">
        <location>
            <begin position="156"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="Disintegrin" evidence="15">
        <location>
            <begin position="396"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="active site" evidence="13">
        <location>
            <position position="326"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="325"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="329"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="335"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ8020457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="666" mass="75489" checksum="CC54699905BD7FFE" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YI19</accession>
    <name>A0A9Q0YI19_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_42589</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000096">
        <property type="protein sequence ID" value="KAJ8019066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YI19"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8019066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="420" mass="45987" checksum="C24510D4B1EB6EA3" modified="2023-09-13" version="1">MGAPAVCERSCGYGCTQQHVLNRTRYSRRELRSRTSFLAHAELETGVAARKFRRCNEKSRAAARACLGKQPAFQTPHAELETGVAARKFRRCNEKSRAAARACLGKQPAFQTPHAELETGVAARKFRRCNEKSRAAARACLGKQPAFQTPHAELETGVAARKFRRCNEKSRAAARACLGKQPAFQTPHAELETGVAARKFRRCNEKSRAAARACLGKQPAFQTPHAELETGVAARKFRRCNEKSRAAARACLGKQPAFQTPHAELETGVAARKFRRCNEKSRAAARACLGKQPAFQTPHAELETGVAARKFRRCNEKSRAAARACLGKQPAFQTPHAELETGVAARKFRRCNEKSRAAARACLGKQPAFQTPHAELETGVAARKFRRCNEKSRAAARACLGKQPAFQTRTSAGLLGGQPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YIM9</accession>
    <name>A0A9Q0YIM9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_38419</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YIM9"/>
    <dbReference type="OrthoDB" id="6250964at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8021269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="334" mass="37916" checksum="DB44F6CCC0122312" modified="2023-09-13" version="1">MDLRSIICYCYPFILSFIVLIGHLESSRQCQSEDVTDNRRGVYGFEHGCIVLRCRLPNQNEPVTISKAGTVFVQDLEKKRPVPRFHYNYKNNNTANQTVHEFKISNISTDDIGTWSFIYNEYIARSIKMNVFIRPSQEEPTCDTVNKFPVYITNDLLEMNCSVQMSDPPVTLELDGLCNITDFSLEAKTHHRVTYSPKSGLSPYSKLCQWGPIKIQKELTVSLNLLSPIIKGNPIGFECVSNVPDAIITWDLPFVDLKQSSSEPGRSSVNVTLPANYAHNTLNISCYAYYRQREISANFVDEISTKKNPNVNASQRFVSCVYLLIAFPLLVQSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YK90</accession>
    <name>A0A9Q0YK90_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tetraspanin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_36517</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the tetraspanin (TM4SF) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YK90"/>
    <dbReference type="OrthoDB" id="10033535at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03127">
        <property type="entry name" value="tetraspanin_LEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1450.10">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000301">
        <property type="entry name" value="Tetraspanin_animals"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF519">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002419">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00259">
        <property type="entry name" value="TMFOUR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="58"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="95"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="213"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="156"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="157"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR002419-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8023934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="245" mass="27115" checksum="CE8670D1596FB16E" modified="2023-09-13" version="1">MESQSCGTRCVQFILFMLNFIFFLCGLGLIVVSIFIWMGLFDTFGINVIDLASILDNVYLEVGCFIMFAVGILVVIFGIVGCCGAFLLNRVLLGLYFLIVLILFFAQLAGAILAALYSAEIDSYAQTEARDLLVKYYNESRSFTIAIDNFQQKYECCGIEVASDYDGITRIGGRHPDSCCPSPVITASQTECTPYERGCREELRDLVVQNTTIIGATLVGVACLELFLMVFAVLLCRNASEKDDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YMW0</accession>
    <name>A0A9Q0YMW0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein FRA10AC1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_32933</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YMW0"/>
    <dbReference type="OrthoDB" id="197967at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019129">
        <property type="entry name" value="Folate-sensitive_fs_Fra10Ac1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050645">
        <property type="entry name" value="Histidine_acid_phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11567">
        <property type="entry name" value="ACID PHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11567:SF25">
        <property type="entry name" value="PROTEIN FRA10AC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09725">
        <property type="entry name" value="Fra10Ac1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="231"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="231"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="258"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="284"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8025388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="318" mass="38290" checksum="CF4541CCF979D5E6" modified="2023-09-13" version="1">MPMEKRRTAMHNQDYGSEFEYEEVQEKKRTRYDLAGSSRVDPGRAKVPSKQFAASEYDREEGKRQRFHYQALNAYDRHKQLVNQYLLYYPGATKEIFQRDTSKDKTDLDVIRENSRFLWSEQDKADETWGKRLAKMYWDKLFKEYCIADLSRYKENKIALRWRIEKEVVEGKGQFICGNKRCAERDDLRTWEVNFGYIEHEVKKNALVKLRLCEICSFKLNYHHKRIEVTRKPSKTKIDKKKKKKEKKRKKHKKHRSGEKSKNKRQEHESSSSSSDSEEDEGQEKEKSYEDIWKGPAVVTEEKSRDEEFDEYFQDLFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YN14</accession>
    <name>A0A9Q0YN14_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_36078</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YN14"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8023594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="92" mass="10777" checksum="22C25E4D8AF0DB2F" modified="2023-09-13" version="1">MLLSAPIATGSLTQPGCTHTYIHTYIHTYIHTYIHTYIHTYIHTYIHTYIHTYTHTHPRFLSGTREMYRRARLLIAATEFSGSYASFELRVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YPP8</accession>
    <name>A0A9Q0YPP8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Cytochrome P450 2U1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HOLleu_34053</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Nanhai2018</strain>
            <tissue evidence="15">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8026259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YPP8"/>
    <dbReference type="OrthoDB" id="1055148at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016712">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008395">
        <property type="term" value="F:steroid hydroxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006805">
        <property type="term" value="P:xenobiotic metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000238">
        <property type="entry name" value="Cytochrome P450 2A6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050182">
        <property type="entry name" value="Cytochrome_P450_fam2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300:SF397">
        <property type="entry name" value="CYTOCHROME P450 2U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300">
        <property type="entry name" value="CYTOCHROME P450 508A4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 13" id="KW-0349">Heme</keyword>
    <keyword evidence="10 13" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0492">Microsome</keyword>
    <keyword evidence="11" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="13">
        <location>
            <position position="414"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ8026259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="468" mass="53692" checksum="DEE20B9832448AEA" modified="2023-09-13" version="1">MAPAHSSHQRDDQKILPPGTRNPFTILRIPKSPPIQLTNLSRRYGPLFTFRFPIVGSLVVVLNNHTVIHEALVDNSDAFPNRVTSPLRHKWGIKGRIVYSYKEEHKIIRQFVLDGMKKYYAGKLHEKVAEEARHFCTAVEEKNGKAFDPGFPLTLATGNINAMLNFGRRYEYSDPNLTKFMEAIDYLHVKLIPSALVQKHPGLYDTFLYRKLKESQEFVVNFICDHIREHRATLDPAIPRDLIDMYLMRVDEENSSENPLFVDERGWFTIKDVITSSSLPSAASLQWVIALLCKHQEMQEKIYEELQAAVGNDRLPEFNDLHKMPFTHAFLTEILRYRTVLPVVSREVVRDVIIDGHTIPQGSNVLINYWAAERDPAVWEKPDDFVPERFLSSDGKTLIKQDSTHALGIGHRDCMGKELAMMQMFLFGTTLLQKFHLKFSPHKKPPSMQGITKTVVVVGKFEVCATKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YQX0</accession>
    <name>A0A9Q0YQX0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">E3 ubiquitin-protein ligase XIAP</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_31778</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YQX0"/>
    <dbReference type="OrthoDB" id="5855668at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00022">
        <property type="entry name" value="BIR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1170.10">
        <property type="entry name" value="Inhibitor Of Apoptosis Protein (2mihbC-IAP-1), Chain A"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001370">
        <property type="entry name" value="BIR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050784">
        <property type="entry name" value="IAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10044:SF139">
        <property type="entry name" value="DEATH-ASSOCIATED INHIBITOR OF APOPTOSIS 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10044">
        <property type="entry name" value="INHIBITOR OF APOPTOSIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00653">
        <property type="entry name" value="BIR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00238">
        <property type="entry name" value="BIR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57924">
        <property type="entry name" value="Inhibitor of apoptosis (IAP) repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50143">
        <property type="entry name" value="BIR_REPEAT_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="311"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="448"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="311"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8026829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="620" mass="71291" checksum="1D64B160165ED9CD" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YS09</accession>
    <name>A0A9Q0YS09_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CARD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_33414</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YS09"/>
    <dbReference type="OrthoDB" id="10185376at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8025769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="154" mass="17895" checksum="0CAE4BE2EB9592C5" modified="2023-09-13" version="1">MAGPSNWEKFPKVLSSRRHFLEENIPLELTSVLIECGIIEPSDEDKLKQIRKTRGLRESQKEFADILIKKENWSEKEVMDFKSCLTELSEVYMIKKIFEEDIEDTVEDETFHSQPHDGFRDGSTTSNISVKGNYNRVVNNSTVTINNRWMVPDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YTG0</accession>
    <name>A0A9Q0YTG0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_31407</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YTG0"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8026547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="109" mass="11564" checksum="750E0A325AA4B37E" modified="2023-09-13" version="1">MEHLNIVCWCSKGHPWVRGKVHLYLPRFVLSAVAFGRGVAFGRGVAFGRGVAFGRGVAFGRGVAFGRGVAFGRGVAFGLSVAFGLSVAFGRGVAFGRVVDLGVCLQWQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1B9E9</accession>
    <name>A0A9Q1B9E9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Fatty acid synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_42099</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000031">
        <property type="protein sequence ID" value="KAJ8019356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1B9E9"/>
    <dbReference type="OrthoDB" id="8010555at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05195">
        <property type="entry name" value="enoyl_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000209">
        <property type="entry name" value="Polyketide synthase Pks12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050444">
        <property type="entry name" value="Polyketide_Synthase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45681:SF6">
        <property type="entry name" value="POLYKETIDE SYNTHASE 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45681">
        <property type="entry name" value="POLYKETIDE SYNTHASE 44-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="4">
        <location>
            <begin position="2"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8019356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="182" mass="20212" checksum="09EA7B288C767A9B" modified="2023-09-13" version="1">MGIVENQGLAYHVFAEPRFLFDVPESWTLEDAATVPTVYCSVFYALVVRGQVKAGETILIHSGAGGVGQAAIAVALSYGCKIYTTVGTQEKRKFLMQKFPTLTQDCFASSRDNSFQRHIMRQTNGSGVNLVLNSLSEELFEASLRCLSKSGRFLEIGKYDLSQNRQIGKNIVHQFNSFFHVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BAJ6</accession>
    <name>A0A9Q1BAJ6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_41245</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8019595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BAJ6"/>
    <dbReference type="OrthoDB" id="10668484at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="217"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="217"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="241"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="279"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8019595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="472" mass="53885" checksum="4D47F9C44043FB15" modified="2023-09-13" version="1">MNPGDQGDPRPMNDKGGAVQQKNRKQGDKSTLPKWFEDSMNMHEHQATYLGKIYEMREKLIKDEKRLLEHSLTRMDRQKILSSRPTLQGRPRAFTSPSLLDDVKGILISRRPDVKTHGKSRNRTSVDDLNNESALKGKRIFKDGKTATFNESSDSETTDRINEEVTEDEVFFKLTKAKRGRNSCDGAFQCKICHEEPRPYLAFDGKERKEFCTAGTERKAKNGTSRRDSRKNVTMTTKRNTTKDYVPEIPSGKRESFSKLPKLKDIPRRKRYETKNKNKSFTSRQKSSSQDCFMGDITGKQININEQCSKPEKDELFSKLESLETDFKGNSPTCLSPRSISPYSLSGASTSPTSPQELKFPENETFPPKVEINLPQVPLNTPKTHSPLLSISANNSELEGDHAPVIVLRPATPYVEKIEQPTKQTKKGWALWQAVRQTEIRRLELKKLIEDIKEFNTNNRSIEQRLSEVTTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BDI7</accession>
    <name>A0A9Q1BDI7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_38662</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BDI7"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040455948" evidence="1">
        <location>
            <begin position="19"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8021453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="309" mass="34521" checksum="9EDB1D8944602FB9" modified="2023-09-13" version="1">MMVHQLLYQALIIQTGLSLFAVNATYVDYVTSGTWNGVGFNIAVKREKIQEKLDAYHSSISDGCGDGPKLVLPEKQVFPELETREHHFVHVDFGYLTSRDFDDFLEDNPHPCVSVSGLEVAVIIPLLAANASGPPIYALALAKLYEKSRGDIPKSNFLYPYIPVDEIQFSDSEGVVKKGSDELRVSIIKHNDPCEPPRSIAEEFDEFFVAEFHFGLPKPDFSFCDRHSFQSDFCTNAKRVQCAVHRHAPNLCQKYNISNPRPCEALVEILNVTSGFRDLILLGNDPVNILSSESYSGDTVVGLKYPCQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BDV7</accession>
    <name>A0A9Q1BDV7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Retrotransposon gag domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_38095</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8023030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BDV7"/>
    <dbReference type="OrthoDB" id="5974147at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33198">
        <property type="entry name" value="ANK_REP_REGION DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33198:SF20">
        <property type="entry name" value="RETROTRANSPOSON GAG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8023030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="215" mass="24480" checksum="B3CF78DE6A55C808" modified="2023-09-13" version="1">MFEGYCIITQLHSHDAVYQKAVFLHCIGPVGVKIYNSLTFADDEDENDIKIIMTKLDTYFIGESNETYERYVFNKRDQQAGETVDAYVANLRTLAKTCNFGDLEESLIKDRLVLGINDPGTRKRLLQTRNLTLQDAVDICKTDEATRNQLKSIDTESVNKVQQKKGIGQQRDKKSVPMIHNCKFCGRSHPKRKEDCPAWGKTCKNCGKKPFCCTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BE58</accession>
    <name>A0A9Q1BE58_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Sulfotransferase 1A4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_39536</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sulfotransferase 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8022128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BE58"/>
    <dbReference type="OrthoDB" id="205623at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11783">
        <property type="entry name" value="SULFOTRANSFERASE SULT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sulfotransferase" evidence="3">
        <location>
            <begin position="47"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8022128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="178" mass="20683" checksum="5074F3159F138E27" modified="2023-09-13" version="1">MAHPETAIEMPAHIKDFLMICDDGSFLIPSTPTSFLPDILKMEMRSSDVIVVTWPKSGTTWMQYIVTQLIYGKEIADEKNLFQTNIFLEIVQNPFQPDDSEGFYKLAEGRPSPRLLKSHLKLSMLPKCLLDSKAKVIYVVRNPKDAAVSYFNFCRMNPFLPPYKDWSEFFEYFRRGLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BHU0</accession>
    <name>A0A9Q1BHU0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription initiation factor TFIID subunit 12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_32293</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BHU0"/>
    <dbReference type="OrthoDB" id="2193432at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07981">
        <property type="entry name" value="HFD_TAF12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000011">
        <property type="entry name" value="Transcription initiation factor TFIID subunit 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037794">
        <property type="entry name" value="TAF12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003228">
        <property type="entry name" value="TFIID_TAF12_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12264">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12264:SF21">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03847">
        <property type="entry name" value="TFIID_20kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription initiation factor TFIID subunit 12" evidence="8">
        <location>
            <begin position="127"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="90"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="109"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8027215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="229" mass="25975" checksum="9BAF24AE60064AFB" modified="2023-09-13" version="1">MNESPTAQSTNNTLLAPSGPPSQASRKTLQETIHDIETQISRFEELEKNASLNTRQEQDLNKLRELLNKALTEQERVKSEKLRQQEVQLQLAKGQGQPVLPNRGGRSGATMSDSSARYSSDNTQVLSKKKIQDLVKEIDPNTQLDEDVEEMLLQIADDFIENIVTAGCQLAKHRKSSTLEVKDVLLHLERNWNMWIPGYSSEESRQHKRPAATESHKQRLALIRKALKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BJB3</accession>
    <name>A0A9Q1BJB3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_29701</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8027685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BJB3"/>
    <dbReference type="OrthoDB" id="425681at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF20">
        <property type="entry name" value="REVERSE TRANSCRIPTASE-LIKE PROTEIN WITH RNA-DIRECTED DNA POLYMERASE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8027685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="93" mass="10874" checksum="72E6A5F1F1273709" modified="2023-09-13" version="1">MAVYNACVISTLLYGSETWMTYAKQERRLNFHLRCIRHILGISWEDKRRLRCLGHIRRMEDGGIPKDILYGELACGKTSTGRPQLRFTDVRET</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BJQ2</accession>
    <name>A0A9Q1BJQ2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Carnosine N-methyltransferase 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_29780</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8027740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BJQ2"/>
    <dbReference type="OrthoDB" id="5956379at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8027740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="191" mass="22315" checksum="BACC43ED3C772AF4" modified="2023-09-13" version="1">MDVSFLPQLDYLSSYYNESFASYKRHTNEYEKMLEWISDRFDAEITSKLLKKENEDGNVTLSVLGIGSGDGKLDVEMLIRLKKKFGTIECTVVEPSAERITSYKKLVQERKDALDGVTFDWRQITLQEFCKISFDRSNKFQFVCAMHSLYYIPKQELDYYIKTLFEWTEGIILMMHGTGRRDALAVFKESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BL32</accession>
    <name>A0A9Q1BL32_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Sodium channel and clathrin linker 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_30710</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BL32"/>
    <dbReference type="OrthoDB" id="551053at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007098">
        <property type="term" value="P:centrosome cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045162">
        <property type="term" value="P:clustering of voltage-gated sodium channels"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034220">
        <property type="term" value="P:monoatomic ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038911">
        <property type="entry name" value="SCLT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031887">
        <property type="entry name" value="SDCCAG8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35970">
        <property type="entry name" value="SODIUM CHANNEL AND CLATHRIN LINKER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35970:SF1">
        <property type="entry name" value="SODIUM CHANNEL AND CLATHRIN LINKER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15964">
        <property type="entry name" value="CCCAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0407">Ion channel</keyword>
    <keyword evidence="3" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="689"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="68"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="179"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="249"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="591"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="689"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="705"/>
            <end position="714"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8028474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="750" mass="86653" checksum="2A8AC1523DF29313" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BME2</accession>
    <name>A0A9Q1BME2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Deleted in malignant brain tumors 1 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_28447</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BME2"/>
    <dbReference type="OrthoDB" id="536948at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000012">
        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000006">
        <property type="entry name" value="neurotrypsin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48071:SF18">
        <property type="entry name" value="DELETED IN MALIGNANT BRAIN TUMORS 1 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48071">
        <property type="entry name" value="SRCR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00530">
        <property type="entry name" value="SRCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00258">
        <property type="entry name" value="SPERACTRCPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00420">
        <property type="entry name" value="SRCR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1 6" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="231"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="10">
        <location>
            <begin position="29"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="9">
        <location>
            <begin position="130"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="54"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="67"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="98"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="156"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8029120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="334" mass="38185" checksum="77CA6A623208E3D9" modified="2023-09-13" version="1">MKFYSCLSVFVFQGCCILSAVMVKLKVDLRLVNGSVPKEGRVEIYYEGQWGTVCDDYWDDADATVVCRQLGLGEFGLAIEKPKFPKGSGKIMLDDVDCTGQENFLVNCSSRPWKENDCRHTEDAGVRCRDILFCKSEPCFNGGTCHERASSYTCSCPENFSGYNCKQQKPSEPPSTTKTHRGKYPMTELNIHDETLTDSTLIYVTRATSLETTRKTLLVTKHPLYSLVSPYMIDIVLSAALLCTIISMFCLMCWVRRKLVTSRVWERRDSFSSDNEMALTLTGHHLEHNLNEYNVQQLQHEEEEEDAISDYERPKSEEEIINESMNKLYENTTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BMS5</accession>
    <name>A0A9Q1BMS5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_28910</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BMS5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040344800" evidence="1">
        <location>
            <begin position="20"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8029500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="175" mass="18629" checksum="62062B4BBFF33DF5" modified="2023-09-13" version="1">MMLTKGLYLLTMAVIEATALLCYTKVLLECNSPGPFCDPLLNDQTPINVTECEDSETMCVLVTGVVTAPGGTLMSSAGSCLPAQEGVDVTNGCFGRSVLNEILPSLGQEITEFVNDNNANLTFIEVCVCDEDLCNGSIRVNTGNDCNDSNDSVKPVYLLPVITSAVMMTLILSFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BMW3</accession>
    <name>A0A9Q1BMW3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Tigger transposable element-derived protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_28752</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BMW3"/>
    <dbReference type="OrthoDB" id="4327074at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050863">
        <property type="entry name" value="CenT-Element_Derived"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004875">
        <property type="entry name" value="DDE_SF_endonuclease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007889">
        <property type="entry name" value="HTH_Psq"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19303">
        <property type="entry name" value="TRANSPOSON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19303:SF71">
        <property type="entry name" value="ZINC FINGER PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03184">
        <property type="entry name" value="DDE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05225">
        <property type="entry name" value="HTH_psq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="219"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="HTH psq-type" evidence="4">
        <location>
            <begin position="16"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="DDE-1" evidence="3">
        <location>
            <begin position="223"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="407"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="527"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="415"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="457"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="542"/>
            <end position="552"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8029374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="578" mass="64300" checksum="ECEE19589E655FFC" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BPE2</accession>
    <name>A0A9Q1BPE2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CRC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_26725</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the lin-54 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BPE2"/>
    <dbReference type="OrthoDB" id="5984512at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005172">
        <property type="entry name" value="CRC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033467">
        <property type="entry name" value="Tesmin/TSO1-like_CXC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46704:SF9">
        <property type="entry name" value="BHLH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46704">
        <property type="entry name" value="CXC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03638">
        <property type="entry name" value="TCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01114">
        <property type="entry name" value="CXC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51634">
        <property type="entry name" value="CRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRC" evidence="4">
        <location>
            <begin position="108"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8030340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="160" mass="17887" checksum="86EB57DAAB1DB6F2" modified="2023-09-13" version="1">MLAAQTSLEEFVCSLYGQSVCQNGNEARYNIFKLNARSESAMPPNQDALRKHILRANYQAAIHKACLKQFPDVPSPIGHGWVISENELSIDWMDLPPAPSGILEYVQCSCKKSKCRQRTCSCTQSGLPCTDLCKCKDCDNAVEVNEIDDIEEYYSEEGDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BRA6</accession>
    <name>A0A9Q1BRA6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_28029</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8031330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BRA6"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="241"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="277"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="309"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="323"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8031330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="396" mass="44810" checksum="D15838D67228C8DD" modified="2023-09-13" version="1">MACTPFYEVVFVCVVLSNLGRSEPISFGIVNPKENQIIFEGEENVTMECKVAMPQNASVKIVLGENVVAHNDHSGNVVQYVIRRVSLEDNGYYACLVTYQDNVIGRITESENFHLVVKNTRPQCIYNTESTDAFSVGAEVIVSCYCMALDACKWNAPEKSNTSFEIYEEVTNGEKFIVRLLVGPLSIDDNDIIFHCSNGPPDSQGCSIGPMLVLPTETNEVTTEPYLVLNTRNDKPEERTILTILIIILIFILILLLVRFLMCLCCKKKERFKHKKLPRKEEETVMLETEPPVQPHIEDRQEQIAELEDKPPTLDLSDDASEHNSLSDSMNITVEPEINQLNEDDDSPEYDKLPPQPVDRLRVAPQSPKPTDDKETFGSFIWKSVSFLNFHKALYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BSL0</accession>
    <name>A0A9Q1BSL0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Small nuclear ribonucleoprotein E</fullName>
            <shortName evidence="12">snRNP-E</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Sm protein E</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_25252</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11 12">Plays a role in pre-mRNA splicing as a core component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BSL0"/>
    <dbReference type="OrthoDB" id="25620at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005687">
        <property type="term" value="C:U4 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000387">
        <property type="term" value="P:spliceosomal snRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01718">
        <property type="entry name" value="Sm_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000013">
        <property type="entry name" value="Small nuclear ribonucleoprotein E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027078">
        <property type="entry name" value="snRNP-E"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11193">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 12" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8 12" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="9 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 12 14" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="7 12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 12" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Sm" evidence="13">
        <location>
            <begin position="16"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00058057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU365053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ8031895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="91" mass="10859" checksum="565B1C0910DB5336" modified="2023-09-13" version="1">MAYKGQKVQKVMVQPINLIFRYLQTRSRIQVWLYDQINIRIEGHIIGFDEYMNLVLDDAEEYHLKNQTRKPLGRILLKGDNITLIQNVQQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BVV2</accession>
    <name>A0A9Q1BVV2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GIY-YIG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_23883</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BVV2"/>
    <dbReference type="OrthoDB" id="10025388at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21301">
        <property type="entry name" value="REVERSE TRANSCRIPTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21301:SF10">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8033590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="310" mass="35565" checksum="C78859D999C5A9DB" modified="2023-09-13" version="1">MIDSLRIKRICSDPLDYDRQIGKLINHFLRSDYPLKVIKKATIKASKVDRNKLLTYSSKTPHQRIPMVFEFNYKLTKLSRPLVKDFKYLRLDPSIRDLFSDPPLSARRQPPNLRSLLTSSRLPQPPASHGNRTCNKRRCQVCKHIITDVPFIPPGTTTKIRPPPLTCDSSNVVYLLVCTRCDRGNYVGETGSKFRLRFNNHKQTIRDNTQGFTVGQHYNLPGHDLNDLKCILLGSSFTSQQIRRNSETKWVLTLNTHIAGLNRDLGIISDYTFHNRGGFRGGPRGPGPPPFQKILKFFQLHKTKDKALFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BWL6</accession>
    <name>A0A9Q1BWL6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_24347</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BWL6"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8033954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="73" mass="8037" checksum="CC7381857A947A0D" modified="2023-09-13" version="1">MLHPLSSLPHPFSLSLAKSSWLPASAFAVLHYVSLLPVYLSVCPIVPRLPTSAVVHRYFHPLSITFPASIPFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BXG5</accession>
    <name>A0A9Q1BXG5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_21559</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BXG5"/>
    <dbReference type="OrthoDB" id="10056585at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37162:SF11">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37162">
        <property type="entry name" value="HAT FAMILY DIMERISATION DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="342"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8034637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="446" mass="51004" checksum="4124A238FE53C30C" modified="2023-09-13" version="1">MHSLFKDSPARSEDYISITGSSKFPSLFCKHRWLENVCVMQRALEVMPQLKKYIEATKQKKVTKPTSKTYDTVCEAVADPLFPTKGEFFISVAGDLEPFLAKYQTDAPMMAFLASDLYSVLKSLMQRFVKQTVLADVTTALRMSKISVEDKKNLKELSNVDVGFVAKNLFSVCKKLLEKSPIKYPIVRTSSWLNPECVASQQVNSKSTLTTCLQLLVDAGYVHARDCDRVLREFQALVDNSSREGSPTPFAAFSRETDRLDTFYYKLQGQTSYSRLWAVVKMMLSLSHGQADVERGFSVNKELTVENQKENSLNARRLISDHLKNVGGVCKVNISKELLSYARNARQRYRQHLEEEAAKKQETKRGEKRKELQREVEQYKKRKAQMESDIKELQREADEKAEAAEHTCDLTLVTKSNALRRHAKEKSEALMDIEKHIALKLKEMQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BYE2</accession>
    <name>A0A9Q1BYE2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_21733</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BYE2"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8034761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="124" mass="14927" checksum="BC29F24C41EB0886" modified="2023-09-13" version="1">MQTQLPTFVQTYIQIFIQIYIQILIQMYIQIFIQIYIQIFIHTFIYKLKHSCKHSNIHSDIHSNIHSDIHSNIHSNIYLNTHSNLHLSKLKHYLEKTFKYTSRYISNIRSIVKSNCHSNKSKHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BZK7</accession>
    <name>A0A9Q1BZK7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">DNA transposase THAP9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_22712</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BZK7"/>
    <dbReference type="OrthoDB" id="7312725at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021896">
        <property type="entry name" value="THAP9-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006612">
        <property type="entry name" value="THAP_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048366">
        <property type="entry name" value="TNP-like_GBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048367">
        <property type="entry name" value="TNP-like_RNaseH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048365">
        <property type="entry name" value="TNP-like_RNaseH_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47577:SF2">
        <property type="entry name" value="THAP DOMAIN CONTAINING 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47577">
        <property type="entry name" value="THAP DOMAIN-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05485">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21788">
        <property type="entry name" value="TNP-like_GBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21789">
        <property type="entry name" value="TNP-like_RNaseH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21787">
        <property type="entry name" value="TNP-like_RNaseH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12017">
        <property type="entry name" value="Tnp_P_element"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00692">
        <property type="entry name" value="DM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00980">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50950">
        <property type="entry name" value="ZF_THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="THAP-type" evidence="8">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="87"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="146"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="93"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8035469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="817" mass="93486" checksum="451967D32C9F4F39" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C2E5</accession>
    <name>A0A9Q1C2E5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_17949</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C2E5"/>
    <dbReference type="OrthoDB" id="10043504at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8037195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="462" mass="53401" checksum="79F2C52F7D094025" modified="2023-09-13" version="1">MSNDKLNWKDAPCHFTWGMDRTVQKVHESSSVFQGENAELYSDDEESCYMFVYLAYLEFLVCRNVDQKFDGSLDKADKIIGKMRDESGKRAFQLVSLANRAWILSMSGESPEAIASVTSQLLKVYGDYLSDNNKKETAYLNAIEAFALRRILLNEKSEEKYQFAIRVIPDIAMWYYELERVAGSSPMMNSSQKTRAKIKHFKKALQFKPDYIRCMVKLIDCYLRVGNVRDARVELNHLEQYDTSGIATAICVRASFYNTVNDNDKALRILFKAEKLNHSDIDKNIAITYILKSKSVSPLKRNKYLKDALTYVEKCLLKHPGSYYGIATKFEVLCLLKESTDIVENFLRKLIVDRPLPPSHLLSLFLKMMKIFEEANKFNPHPDHEIVLTLYENMVQVALQGCLKNDDNGEMILSATVKVILNEAKTFVDRYLKKNRNVLNERNERFLELNERLLPYLNNDAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C3T9</accession>
    <name>A0A9Q1C3T9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">unspecific monooxygenase</fullName>
            <ecNumber evidence="5">1.14.14.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HOLleu_18540</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Nanhai2018</strain>
            <tissue evidence="17">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 14">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.14.1" evidence="5"/>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C3T9"/>
    <dbReference type="OrthoDB" id="1055148at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004508">
        <property type="term" value="F:steroid 17-alpha-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042446">
        <property type="term" value="P:hormone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042448">
        <property type="term" value="P:progesterone metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000238">
        <property type="entry name" value="Cytochrome P450 2A6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24289:SF21">
        <property type="entry name" value="CYTOCHROME P450 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24289">
        <property type="entry name" value="STEROID 17-ALPHA-HYDROXYLASE/17,20 LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 14 15" id="KW-0349">Heme</keyword>
    <keyword evidence="11 14 15" id="KW-0408">Iron</keyword>
    <keyword evidence="13 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 14 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0492">Microsome</keyword>
    <keyword evidence="12 15" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="10 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="14">
        <location>
            <position position="478"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ8037659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="537" mass="60662" checksum="8BC36B0EB9862E48" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C3Z0</accession>
    <name>A0A9Q1C3Z0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_18627</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C3Z0"/>
    <dbReference type="OrthoDB" id="5586934at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.100">
        <property type="entry name" value="TspO/MBR protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038330">
        <property type="entry name" value="TspO/MBR-related_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33802">
        <property type="entry name" value="SI:CH211-161H7.5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33802:SF1">
        <property type="entry name" value="XK-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="125"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8037734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="163" mass="18572" checksum="5D96D8D9CE2DF028" modified="2023-09-13" version="1">MAQDIRFIGLSVIVVLTFGFALFVNYLAGAGKDAVIPVFDSSIGQISDKYENPVTPADWTFAIWGLIYPWQFALITYVLSTICRNNEDGNPLYQYPPVISYPFLAIYGLNLLCNAGWCYVFCNQLMVYALVAIVLMALTLYYALLDNSVRVYNYYAVLYKRYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C487</accession>
    <name>A0A9Q1C487_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Elongation of very long chain fatty acids protein</fullName>
            <ecNumber evidence="10">2.3.1.199</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Very-long-chain 3-oxoacyl-CoA synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_18798</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a very-long-chain acyl-CoA + malonyl-CoA + H(+) = a very-long-chain 3-oxoacyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:32727"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:90725"/>
            <dbReference type="ChEBI" id="CHEBI:90736"/>
            <dbReference type="EC" id="2.3.1.199"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the ELO family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.199" evidence="10"/>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C487"/>
    <dbReference type="OrthoDB" id="10259681at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009922">
        <property type="term" value="F:fatty acid elongase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034625">
        <property type="term" value="P:fatty acid elongation, monounsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034626">
        <property type="term" value="P:fatty acid elongation, polyunsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019367">
        <property type="term" value="P:fatty acid elongation, saturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030457">
        <property type="entry name" value="ELO_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002076">
        <property type="entry name" value="ELO_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157:SF17">
        <property type="entry name" value="ELONGATION OF VERY LONG CHAIN FATTY ACIDS PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157">
        <property type="entry name" value="FATTY ACID ACYL TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01151">
        <property type="entry name" value="ELO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01188">
        <property type="entry name" value="ELO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="5 10" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2 10" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7 10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="55"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="107"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="134"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="158"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="190"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="228"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="263"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="263"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8037869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="285" mass="33210" checksum="97D648D3A2EE5C75" modified="2023-09-13" version="1">MILSAVEQFAASYDERAILERISRHWMKTLPISAIYIILVFSGKRWMKDRPKFKLRWALTLWSAALALFSMFGAWFMLVRTVKDLREFGFRYTVCDNTFLFTGHGVWLYWFCLSKIVELGDTFFIVVRKQKLIFLHWYHHMVTLIYSCYAYGSGVAAGRYFAGVNFAIHTLMYSYYAIRASGLVRMPRQVNITLTSLHLLQMGFGVYLNIYAYTELTSGRPCDNTWPSIYSSFLMYASYVALFANFFYQNYLVKSKSDPKVNNNLHSGARQNESISHQANPKKEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C4C8</accession>
    <name>A0A9Q1C4C8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phospholipase A2</fullName>
            <ecNumber evidence="8">3.1.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_16636</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1-acyl-sn-glycero-3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15801"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="5">Binds 1 Ca(2+) ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the phospholipase A2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.4" evidence="8"/>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C4C8"/>
    <dbReference type="OrthoDB" id="5841574at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047498">
        <property type="term" value="F:calcium-dependent phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050482">
        <property type="term" value="P:arachidonate secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00125">
        <property type="entry name" value="PLA2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.90.10:FF:000007">
        <property type="entry name" value="Acidic phospholipase A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.90.10">
        <property type="entry name" value="Phospholipase A2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001211">
        <property type="entry name" value="PLA2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016090">
        <property type="entry name" value="PLA2-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033112">
        <property type="entry name" value="PLA2_Asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033113">
        <property type="entry name" value="PLA2_histidine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036444">
        <property type="entry name" value="PLipase_A2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11716:SF51">
        <property type="entry name" value="PHOSPHOLIPASE A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11716">
        <property type="entry name" value="PHOSPHOLIPASE A2 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00068">
        <property type="entry name" value="Phospholip_A2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00389">
        <property type="entry name" value="PHPHLIPASEA2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00085">
        <property type="entry name" value="PA2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48619">
        <property type="entry name" value="Phospholipase A2, PLA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00119">
        <property type="entry name" value="PA2_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00118">
        <property type="entry name" value="PA2_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0106">Calcium</keyword>
    <keyword evidence="3 6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040540181" description="Phospholipase A2" evidence="8">
        <location>
            <begin position="19"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipase A2-like central" evidence="9">
        <location>
            <begin position="24"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="70"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="124"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="51"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="66"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="73"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="83"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="110"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR601211-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR601211-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR601211-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8039048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="148" mass="16071" checksum="979191A72D7FAA77" modified="2023-09-13" version="1">MKMIILFLLVGSASFTSSLRARRSVIQFGQMIECVTPLSALTDYNGYGCWCGYGGTGTPLDGTDRCCKTHDECYNALKDSGTCSSWNQIYSESYSFSCYNCDTPSFAISCGSGTSCAVGLCQCDKTAAECFSGETFNSAYRNYNKNNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C623</accession>
    <name>A0A9Q1C623_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Purpurin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_16952</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C623"/>
    <dbReference type="OrthoDB" id="565904at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005501">
        <property type="term" value="F:retinoid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034632">
        <property type="term" value="F:retinol transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00191">
        <property type="entry name" value="TY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.800.10">
        <property type="entry name" value="Thyroglobulin type-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012674">
        <property type="entry name" value="Calycin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022272">
        <property type="entry name" value="Lipocalin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000566">
        <property type="entry name" value="Lipocln_cytosolic_FA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002449">
        <property type="entry name" value="Retinol-bd/Purpurin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000716">
        <property type="entry name" value="Thyroglobulin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036857">
        <property type="entry name" value="Thyroglobulin_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11873:SF0">
        <property type="entry name" value="LIPOCALIN-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11873">
        <property type="entry name" value="RETINOL-BINDING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08212">
        <property type="entry name" value="Lipocalin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00086">
        <property type="entry name" value="Thyroglobulin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01174">
        <property type="entry name" value="RETINOLBNDNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00211">
        <property type="entry name" value="TY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50814">
        <property type="entry name" value="Lipocalins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57610">
        <property type="entry name" value="Thyroglobulin type-1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00213">
        <property type="entry name" value="LIPOCALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51162">
        <property type="entry name" value="THYROGLOBULIN_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040205542" evidence="3">
        <location>
            <begin position="21"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="Thyroglobulin type-1" evidence="4">
        <location>
            <begin position="205"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="242"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8039287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="273" mass="30846" checksum="859A78DCAB2B45DC" modified="2023-09-13" version="1">MFRFALLACLYLSLCASAQSCQVSEISVQENFDPKKYVGTWYVLAHVQDGVYPYQRRSHYHLNDDMTFSMQTQRVVEPATCEGWHFEAHGRNPDSTKKGKLLIRPNIDIMEVEPEDYWIISTDYIHYALVYSCSEVLDDGTCDPGQTHAWIMGRSPLPLKSEIKKHVLNKMETELCLHIDRITEIPTECDSSLGVPTIPIEGKVFVDCTSHLESIMAGADPLTTTYVPQCAEDGIRYLPRQCDNNLETCWCVDEESGRKIEGTAATVPDTPPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C6C9</accession>
    <name>A0A9Q1C6C9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Complex I-B14.5a</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">NADH-ubiquinone oxidoreductase subunit B14.5a</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HOLleu_14358</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Nanhai2018</strain>
            <tissue evidence="15">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Complex I is composed of 45 different subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complex I NDUFA7 subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C6C9"/>
    <dbReference type="OrthoDB" id="10063829at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006120">
        <property type="term" value="P:mitochondrial electron transport, NADH to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009947">
        <property type="entry name" value="NDUA7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12485:SF1">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12485">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07347">
        <property type="entry name" value="CI-B14_5a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="9" id="KW-0249">Electron transport</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00030360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00033401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ8040143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="113" mass="12798" checksum="4F89BDD532F54F6A" modified="2023-09-13" version="1">MATATRLIQRLRNFLVGRNLQAKLNNRTVDEIASRTIPEPNLPEGCSHKLAANYYYTRDGRRELNPPTVVYSQTKLLQEPQQGESASSVAQARKVVTPGVMRQWKLSTDEPFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C710</accession>
    <name>A0A9Q1C710_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Caveolin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_14816</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cell membrane</location>
            <topology evidence="1 6">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Golgi apparatus membrane</location>
            <topology evidence="6">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Membrane</location>
            <location evidence="6">Caveola</location>
            <topology evidence="6">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the caveolin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C710"/>
    <dbReference type="OrthoDB" id="5917823at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005901">
        <property type="term" value="C:caveola"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042383">
        <property type="term" value="C:sarcolemma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070836">
        <property type="term" value="P:caveola assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051480">
        <property type="term" value="P:regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001612">
        <property type="entry name" value="Caveolin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10844">
        <property type="entry name" value="CAVEOLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10844:SF13">
        <property type="entry name" value="CAVEOLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01146">
        <property type="entry name" value="Caveolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5 6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="21"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8040503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="100" mass="11676" checksum="D6ED7CFF4D993F08" modified="2023-09-13" version="1">MRSFNSVWRLSHLTFINMKLWCYRIITLIFAVPAAILWGLYFALLTFMYIWCVVPCIKATFIKIQCAGQLFGILVRTFVEPFYIAFGKVFSNIHITVAKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C9M6</accession>
    <name>A0A9Q1C9M6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_15119</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 29 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C9M6"/>
    <dbReference type="OrthoDB" id="10264956at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008373">
        <property type="term" value="F:sialyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1480.20">
        <property type="entry name" value="Glycosyl transferase family 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001675">
        <property type="entry name" value="Glyco_trans_29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038578">
        <property type="entry name" value="GT29-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23136:SF12">
        <property type="entry name" value="ALPHA-2,6-SIALYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23136">
        <property type="entry name" value="TAX1-BINDING PROTEIN 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00777">
        <property type="entry name" value="Glyco_transf_29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040136509" evidence="11">
        <location>
            <begin position="17"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8040733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="148" mass="17090" checksum="347EB0D8471000D9" modified="2023-09-13" version="1">MFPLLLLLQVIQHCKKCAIVTSSGHLLGTKAGPEIDSTECVIRMNAAPTVKYEADVGQRTTFRIVGHRNFPRMFDSEPERQSYFVNPKTKSDAIVTAWLYAVNVGRNIETFLSKKYSKKYKDVKFFITSEEQMIWNEKLFYQMLGIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CA65</accession>
    <name>A0A9Q1CA65_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BEN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_15222</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CA65"/>
    <dbReference type="OrthoDB" id="10069100at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2590">
        <property type="entry name" value="BEN domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018379">
        <property type="entry name" value="BEN_domain"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51457">
        <property type="entry name" value="BEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BEN" evidence="2">
        <location>
            <begin position="204"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="161"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8040819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="314" mass="34111" checksum="255C890ACE60B628" modified="2023-09-13" version="1">MAGTGVGSGSTINYPGEEEIVSFQKGLHVEQTVLCSWGSRKYRAIVKSIEGKGKGPDADVYEQSTFRPTPEANDILTDVFVSPSPPSSPGIEEMTTAVSTQTDNSDAPSQPNFSLNAGTQTDGYYVTHEFMCALQEQLQNLAKSNASLNAKMDAMSDLLGRSSTLTPASPSRSPFTPPAPSLPSRREQPSYGKLVNGNIQMRVENGSVPFVEIPKQTYERIFYVAKTATSFVRQLLPLVYSREELVASNFEGGEVLTGNGYVTKPALERPRMASLLGQVELEFPGSTFGKANMGKIREAINAHCRTEARKKLLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CAH8</accession>
    <name>A0A9Q1CAH8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Poly [ADP-ribose] polymerase</fullName>
            <shortName evidence="15">PARP</shortName>
            <ecNumber evidence="15">2.4.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">HOLleu_11835</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">Nanhai2018</strain>
            <tissue evidence="21">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>NAD(+) + (ADP-D-ribosyl)n-acceptor = nicotinamide + (ADP-D-ribosyl)n+1-acceptor + H(+).</text>
            <dbReference type="EC" id="2.4.2.30"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the ARTD/PARP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.-" evidence="15"/>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CAH8"/>
    <dbReference type="OrthoDB" id="429950at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003950">
        <property type="term" value="F:NAD+ poly-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990404">
        <property type="term" value="F:NAD+-protein mono-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070212">
        <property type="term" value="P:protein poly-ADP-ribosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01437">
        <property type="entry name" value="parp_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08003">
        <property type="entry name" value="WGR_PARP2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.142.10:FF:000001">
        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.228.10:FF:000002">
        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.720.50">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.228.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.142.10">
        <property type="entry name" value="Poly(ADP-ribose) polymerase, regulatory domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050800">
        <property type="entry name" value="ARTD/PARP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012317">
        <property type="entry name" value="Poly(ADP-ribose)pol_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004102">
        <property type="entry name" value="Poly(ADP-ribose)pol_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036616">
        <property type="entry name" value="Poly(ADP-ribose)pol_reg_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036930">
        <property type="entry name" value="WGR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008893">
        <property type="entry name" value="WGR_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018123">
        <property type="entry name" value="WWE-dom_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004170">
        <property type="entry name" value="WWE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037197">
        <property type="entry name" value="WWE_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10459">
        <property type="entry name" value="DNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10459:SF60">
        <property type="entry name" value="POLY [ADP-RIBOSE] POLYMERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00644">
        <property type="entry name" value="PARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02877">
        <property type="entry name" value="PARP_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05406">
        <property type="entry name" value="WGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02825">
        <property type="entry name" value="WWE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00773">
        <property type="entry name" value="WGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00678">
        <property type="entry name" value="WWE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56399">
        <property type="entry name" value="ADP-ribosylation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47587">
        <property type="entry name" value="Domain of poly(ADP-ribose) polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142921">
        <property type="entry name" value="WGR domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117839">
        <property type="entry name" value="WWE domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51060">
        <property type="entry name" value="PARP_ALPHA_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51059">
        <property type="entry name" value="PARP_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51977">
        <property type="entry name" value="WGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50918">
        <property type="entry name" value="WWE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0013">ADP-ribosylation</keyword>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 15" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 15" id="KW-0520">NAD</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="WWE" evidence="17">
        <location>
            <begin position="84"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="WGR" evidence="20">
        <location>
            <begin position="252"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="PARP alpha-helical" evidence="19">
        <location>
            <begin position="380"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="domain" description="PARP catalytic" evidence="18">
        <location>
            <begin position="505"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="163"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="360"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="170"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="197"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00033987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU362114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAJ8041109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="729" mass="82951" checksum="9B4E86BCC3D6C33D" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CAY6</accession>
    <name>A0A9Q1CAY6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">beta-glucosidase</fullName>
            <ecNumber evidence="3">3.2.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_12882</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the glycosyl hydrolase 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.21" evidence="3"/>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CAY6"/>
    <dbReference type="OrthoDB" id="65569at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000013">
        <property type="entry name" value="lactase-phlorizin hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033132">
        <property type="entry name" value="GH_1_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001360">
        <property type="entry name" value="Glyco_hydro_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018120">
        <property type="entry name" value="Glyco_hydro_1_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353">
        <property type="entry name" value="GLYCOSYL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353:SF36">
        <property type="entry name" value="LP05116P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00232">
        <property type="entry name" value="Glyco_hydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00131">
        <property type="entry name" value="GLHYDRLASE1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00572">
        <property type="entry name" value="GLYCOSYL_HYDROL_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00653">
        <property type="entry name" value="GLYCOSYL_HYDROL_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 9 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="7">
        <location>
            <position position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8041942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="496" mass="56936" checksum="5C2F697F5136129B" modified="2023-09-13" version="1">MAQSTNNAQYIYDQFNDPERDAFINGTFPEEFAWGCATAAYQVEGGHDADGKGEGAWDTFSHSDGKIFNDHTGDVACDSYNKMDEDVQLLKNLGVSHYRFSLSWARILPTGFVNEVNQAGVQYYHKLLDALAVANIKPMVTIHHFDLPKSLDNLGGWHNELMAVYFNMFADFCFREFGSKVKLWITINEPTVFAIYGYEFGKFPPGWTHLGSGAYRAVCIMLKAHAMAYRTYDTKYRKSQGGLVSIALNSFWDEPKTDSDADKAAAERFQQFELGSVAKPIFVDGDTPDVVKKYVDRKSRQLGLLCSRIPSFNEDERQLLKGTADYFALNYYSTRIVNHKDYDPQSLLVPNIFSDYDLEESWDKNFPRAFSEWLYSAPWGFRKLLNWIKKNYGDVPIYITENGFSEKDGPMRLNDTERVKYYKSHINEMLKARLLDGVNVQGYFAWSLMDNFEWAEGYNERFGLHHVDFNDPERPRRAKASSKVYAKIIKDNGFPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CBT2</accession>
    <name>A0A9Q1CBT2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cystine/glutamate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_12986</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8042022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CBT2"/>
    <dbReference type="OrthoDB" id="10062876at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050598">
        <property type="entry name" value="AminoAcid_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785:SF375">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="110"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="136"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8042022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="202" mass="22607" checksum="AE7C1D1157AC5B1A" modified="2023-09-13" version="1">MSHSGIASLVYSVSHCRTIVRAMAISFVCVTILYLLTNVAYFAVLTTEEIMSSDAVAATPITVLVIYSGNIWTVIDFLYCFAQIENILTIAIVPYLRWKKPERVLSYKAPLWSCVVFIAFTTVVLLITAYSNPIQIGITVAIICIALAYYWLIQLINKSDRFQAKLSKLSYKTATLLGPGSLYLEVTFAIPSSPVPYYPRYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CBV1</accession>
    <name>A0A9Q1CBV1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_12795</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CBV1"/>
    <dbReference type="OrthoDB" id="9048260at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24376:SF235">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24376">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="272"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="300"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="192"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="192"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="205"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8041873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="332" mass="37526" checksum="523FBF5FB140EE14" modified="2023-09-13" version="1">MDIVTIKVEPEDFQWDEKSNGRINSSARHATILENGGAETVKKEDASDSSQVVGSCVITKGESVSPKFVDGSQIIFKKEEERDPSLETGMENITIQVNVQVEEEELLEEEKENEIAVATRNEDSDLLEEGFGLPPLQLDGDHKQLVLNYPMPLAETSSESTFPSASSSKFIAKGDDCVPLNKFPKKVKLAINERQRKSDNRPSSRRSGTGNKFVTSDLENQHHCLENPVSKSEGGRTCTLGKSCQCEWCEKRFILKSNCDKSEVIYSGVKHFKCQFCEKSFQTKRQNKRHEAMHTSKKPFQCQHCAKPFQTKWECKRHEKIHTDDTLQVPVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CBW0</accession>
    <name>A0A9Q1CBW0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Cystine/glutamate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_12985</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8042021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CBW0"/>
    <dbReference type="OrthoDB" id="5982228at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050598">
        <property type="entry name" value="AminoAcid_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785:SF375">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="56"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="86"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8042021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="152" mass="16007" checksum="4435FD8F30EF230B" modified="2023-09-13" version="1">MTEDGEVIPGDKVVLPRFLGLIQSISFILGLIIGTGIFLTPTGVLRGSSGSIGVSLIMWVVGAFMSSTGAMCMTEMAIYFRQSGGSYIFLSSVYGPVVGFVKVWVSFFVYSPCSSAIQSFAIINFLTTPFAGACGSIPDVGTKIGASCVFRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CDY9</accession>
    <name>A0A9Q1CDY9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phosphatidylinositol 4-kinase alpha</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_09622</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8042769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CDY9"/>
    <dbReference type="OrthoDB" id="10264149at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8042769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="368" mass="41390" checksum="F23F363EA5330FF8" modified="2023-09-13" version="1">MYDYEAAEFAGNMVKPGSSVFGNSLIFLARSLAKIDDTPWEKAPLMHPGSYIVDSRRSDAVIALGIFLLESELKFKEKIVQYLLNLLKGLPSACWLDDNSHSTVQCLPLSENFTFCLTAILSDVAVRDEDLKDQIVHAQMEVLQALAMTCQQPGDITEEILCRHVIPAFIGMIRAMCLTQDSENGVPLFCSFFPSGTLKPKKQESSIDSMDHLTYNSFRPIIYPAMVPHIKALSDPSSSAQFCLKNHSLENVDNSENSVISSDGQFAYLFQVVGAIYKHKESIEKEKKETEGSFIELSLHQLQSFLSVARSMLQEKVLQSLDDMASKISHLGLRDFPYKSFCEIISFVFITYFNHLIEVQSGEFYMKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CEJ1</accession>
    <name>A0A9Q1CEJ1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Synaptic vesicle 2-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_11332</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CEJ1"/>
    <dbReference type="OrthoDB" id="4139357at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511:SF5">
        <property type="entry name" value="MAJOR FACILITATOR-TYPE TRANSPORTER HXNZ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511">
        <property type="entry name" value="SYNAPTIC VESICLE GLYCOPROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="41"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="80"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="109"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="43"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8043998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="245" mass="28029" checksum="296CACB341E60A72" modified="2023-09-13" version="1">METNDRMEMEQLAYGSHEVKESKVYTIQEAVNTVGNGWFQLWTGVVCGLIWVADAIALLLLSIMAVVLRCEWQLTVFQSSMVTAIPFLCQIIGAYVWGIYSDKYGRKSILFLTTALLFWFSVLSACSTDITWFLICRCVSTFMIGGSPQAITFFMEFLPTKKRGFFVAIFETLPESPYYHVSIGAAEKVHKTFRTIAKWNRKDPISGGIKMDIQEVKSRVRQYTAHNDQKFKPMSDTFKNDKPHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CFU6</accession>
    <name>A0A9Q1CFU6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Kelch domain-containing protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_07359</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CFU6"/>
    <dbReference type="OrthoDB" id="10251809at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011043">
        <property type="entry name" value="Gal_Oxase/kelch_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46228">
        <property type="entry name" value="KELCH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46228:SF2">
        <property type="entry name" value="KELCH REPEAT PROTEIN (AFU_ORTHOLOGUE AFUA_4G14350)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50965">
        <property type="entry name" value="Galactose oxidase, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8044576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="381" mass="43728" checksum="6505A1FB0D2938C8" modified="2023-09-13" version="1">MESDIRDDNKLPAERSGHVATQLNGKMIVWGGFGALSDESDRISEKYIASNILWIYNIEAQTWVQHFVDCPMTGQSGSGCFSFGGKLFVWGGYPHHSKQMFVLDPAVGIWQDAQVEGDPPEYRDKFVSWLYKDRYVIFGGFGPAPENATRESWVADPSSYHHNSVIRGWNNQLYEYNIRENRWSKPQTRGPVPCPRASHAGAIVENRGYVFGGRFQERRRNDIHFVDLDTYSWSDRLPVHGPQPEGRSWHSLTAVGSHHLLLCGGFNTACKPLDDAWMFDTNEFLWKRLEWDLPSPRLWHTAVQGTHLGEVVIFGGSTNNILSLEEPTRHTNSVLHIFVTPKPLFRICCDMIRENLERLRPQINQLPSYLQKKFDQNPMPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CFZ6</accession>
    <name>A0A9Q1CFZ6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ryncolin-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_11031</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CFZ6"/>
    <dbReference type="OrthoDB" id="9990035at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00087">
        <property type="entry name" value="FReD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050373">
        <property type="entry name" value="Fibrinogen_C-term_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025615">
        <property type="entry name" value="TILa_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040941">
        <property type="entry name" value="GGGWT_bact"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143">
        <property type="entry name" value="FIBRINOGEN/TENASCIN/ANGIOPOEITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12714">
        <property type="entry name" value="TILa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00186">
        <property type="entry name" value="FBG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040507065" evidence="1">
        <location>
            <begin position="28"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="2">
        <location>
            <begin position="36"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="2">
        <location>
            <begin position="284"/>
            <end position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8043774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="527" mass="59843" checksum="5CBDDA9D59A43B05" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CG23</accession>
    <name>A0A9Q1CG23_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_11693</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8044275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CG23"/>
    <dbReference type="OrthoDB" id="418169at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040446049" evidence="1">
        <location>
            <begin position="22"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8044275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="306" mass="34704" checksum="25A877777EF8F026" modified="2023-09-13" version="1">MATKIPVLMTFVLLWMNVVYCMEQIHLKDLRQLRIQGIASRITVSRNALKDFLDNITGVCDLEPFLFKSGSNENLYDPSLIGEVDIGYFVIHDHTLNGISYKLKEKVATSYRLAGVSVPFVSGGRHGFLILALFTDSDKKVFFPYSTNHDHITYIDEIEETEDGTITLRHGNDVLVMNTQHNDTCGDNQRQHVRQDFDKILGKQVTGTDYPLPFDWGCDVLGDDVDFCDILKENKNELEKAGPNVCTEMTITSQSKRCPATMEFQEITGFFRNALPFLSTYFGSFEAEWIDREHGGGIIFPCLIFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CG91</accession>
    <name>A0A9Q1CG91_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_07617</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CG91"/>
    <dbReference type="OrthoDB" id="1917519at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8044771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="70" mass="8252" checksum="20C207BD34E9BF47" modified="2023-09-13" version="1">MSRGRTLLFLVEVKGHPGSLEVNLRKPRKRLVNTISRDSNDRHFSYLVYRFVMLKGRTLLFLVEVKGHLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CGB5</accession>
    <name>A0A9Q1CGB5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Protein STPG4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_07612</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CGB5"/>
    <dbReference type="OrthoDB" id="6228811at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001939">
        <property type="term" value="C:female pronucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042585">
        <property type="term" value="C:germinal vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001940">
        <property type="term" value="C:male pronucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044727">
        <property type="term" value="P:epigenetic programing of male pronucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010736">
        <property type="entry name" value="SHIPPO-rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35678">
        <property type="entry name" value="PROTEIN STPG4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35678:SF1">
        <property type="entry name" value="PROTEIN STPG4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07004">
        <property type="entry name" value="SHIPPO-rpt"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="18"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8044767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="284" mass="32267" checksum="CDDD95E0237771E3" modified="2023-09-13" version="1">MAPKLTPEATSLGEVPEEVERPTSTEGKLKKPPSLDKNRKGTSTPGSKERVNVWKRDTPKKGRKCLSVDYEKEVSGREQWWRKDIRITPVPGSYETETFLKEFEVRPATYAFKNEGRKKDADVIRKGAYLLPGAYEKHDLIFDLSKTRNTYTFKNSKRSKDLPIVKDKDINVCPTAYEMDSYLSVSVRDGKNKHANFKSASMRFPTIYFKGTKNPAPCHYEYRSPPVLHTVTSSFKSRTPRFSSSHTKVPGPGTYDSTYQSPMPATIAKMGRNHGLFFTSAFQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CGP7</accession>
    <name>A0A9Q1CGP7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">2',3'-cyclic-nucleotide 3'-phosphodiesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_11548</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8044164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CGP7"/>
    <dbReference type="OrthoDB" id="3231855at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004113">
        <property type="term" value="F:2',3'-cyclic-nucleotide 3'-phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009214">
        <property type="term" value="P:cyclic nucleotide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1740.10">
        <property type="entry name" value="2',3'-cyclic nucleotide 3'-phosphodiesterase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008431">
        <property type="entry name" value="CNPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047325">
        <property type="entry name" value="CNPase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009097">
        <property type="entry name" value="Cyclic_Pdiesterase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10156">
        <property type="entry name" value="2',3'-CYCLIC-NUCLEOTIDE 3'-PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10156:SF0">
        <property type="entry name" value="2',3'-CYCLIC-NUCLEOTIDE 3'-PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05881">
        <property type="entry name" value="CNPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55144">
        <property type="entry name" value="LigT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0488">Methylation</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-0636">Prenylation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040445666" evidence="10">
        <location>
            <begin position="25"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide phosphodiesterase catalytic" evidence="11">
        <location>
            <begin position="172"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="310"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="324"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF05881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8044164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="411" mass="47258" checksum="B2F8CCFB7BB45EE3" modified="2023-09-13" version="1">MFRRGNRLLNLGLPLLTLLRVGLSSPNQRATCPAFSFLSHDFFCQMSHEMEGQVSSKKKNYFPFLNDQPTIDYIKTSRLMMYLPECSDILDSIKHVYPEAKIFSEQSSCSDQTTSAVLNEVKKWLESFSNESAVFAVINDPRLITTEKLKPFGNLSVAFRLVQVYVDQTNFIPLFYGWFFNLPDSEDLRQQGHELLKHLNKEEKFVEEFSKHAIDGPTKGKVDICKYFDTRTLVVGQTLLHCTAFFTNFKQMKETEDYNNHHRQDITRAFKMQIIGFLVTPRTFGVRLKLGDKELQLWHDDIQVAQEVTPQDGNKDEGWKPSKYSPTNGRGSTSHLTLAVREDISAAETGRDLKEIIKREVENQKSGKKVLTLELEIGDARCYGDGLWAIYLNKPQTFTALFSNYYVPEPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CHC3</accession>
    <name>A0A9Q1CHC3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Dehydrogenase/reductase SDR family member 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_07542</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CHC3"/>
    <dbReference type="OrthoDB" id="47007at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053011">
        <property type="entry name" value="SDR_family_member_7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44269">
        <property type="entry name" value="DEHYDROGENASE/REDUCTASE SDR FAMILY MEMBER 7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44269:SF1">
        <property type="entry name" value="DEHYDROGENASE_REDUCTASE SDR FAMILY MEMBER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="12"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8044720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="352" mass="39433" checksum="106FFC8101AA49BC" modified="2023-09-13" version="1">MELYKIPVLGVLGVVTLVVFPLLHLITFFFLLGLIVYVFLIVFSDGDVMLMFYEWFGSKGDHLKGKVVWITGGSSGLGEELAVLMAKKGVKLILTARREKELERVKQRCIQQSELKDEDILIMKMDGVAFDTHLKCVDNALEYFKKIDILVNNSGRSQRSIAVDADLSTTEFILKLNLLGAISITKCVLPHMIANRSGHIVNMSSVAGKLCAPCSTAYSASKFGMNGFFDTLRAEVHYHNIVITNVCPGPVATEIIENAVQKDFDQVPCRNYDGIGERTWKILQTPQRCVELTLVAISNQLSEVWIAKTPMLTQFYLAQYTPTTFRMLSERLGYLRVEAYLKAQEQANSGMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CI35</accession>
    <name>A0A9Q1CI35_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Inversin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_07785</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CI35"/>
    <dbReference type="OrthoDB" id="5806726at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070531">
        <property type="term" value="C:BRCA1-A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031436">
        <property type="term" value="C:BRCA1-BARD1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004563">
        <property type="term" value="F:beta-N-acetylhexosaminidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0085020">
        <property type="term" value="P:protein K6-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.379.10">
        <property type="entry name" value="Chitobiase/beta-hexosaminidase domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025705">
        <property type="entry name" value="Beta_hexosaminidase_sua/sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029018">
        <property type="entry name" value="Hex-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015882">
        <property type="entry name" value="HEX_bac_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171:SF11">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02838">
        <property type="entry name" value="Glyco_hydro_20b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00738">
        <property type="entry name" value="GLHYDRLASE20"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55545">
        <property type="entry name" value="beta-N-acetylhexosaminidase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="58"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="142"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="175"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-hexosaminidase bacterial type N-terminal" evidence="7">
        <location>
            <begin position="311"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="239"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="242"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8044899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="929" mass="100875" checksum="EF2F3744E91028DA" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CJD8</accession>
    <name>A0A9Q1CJD8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Serine/arginine-rich splicing factor 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_08868</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the splicing factor SR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CJD8"/>
    <dbReference type="OrthoDB" id="1099063at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000028">
        <property type="entry name" value="Putative serine/arginine-rich splicing factor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050374">
        <property type="entry name" value="RRT5_SRSF_SR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23003">
        <property type="entry name" value="RNA RECOGNITION MOTIF RRM DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23003:SF51">
        <property type="entry name" value="SERINE-ARGININE PROTEIN 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 9" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="11">
        <location>
            <begin position="6"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="11">
        <location>
            <begin position="130"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="76"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="196"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="97"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="204"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="283"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8045785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="314" mass="35917" checksum="AE744F342C845C26" modified="2023-09-13" version="1">MPGGPGCVYLGNLSHRAQESDVEKLLRPYGKLRYVSLKSKYGFAEFEDSRDADDAVYELNGRELFGERLIVEHARGPGRRRDRGDDRYGGGGGGYRDGPRRGGFDRYGSRGGQRQNRDGDKYGPPVRTEYRLIVENLSSKVSWQDLKDYVRQAGEVTFADAHKQRKNEGIVEFSSRYDMENAIKKLDNTELNGRKIKLVEDRPSRKRSHSRSRSRSRRRSRSRSRSHSNSRSRSRSRSRSKSKSPKRSKSKSRSKSRSRSKSKSKSRSRSPPPKKSRSRSPSPKRDDEKKSPEKSPARSKSRSRSKSQSPSKDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CJM8</accession>
    <name>A0A9Q1CJM8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">IQ motif and SEC7 domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_05220</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the BRAG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="430364at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032012">
        <property type="term" value="P:regulation of ARF protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00171">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1000.11:FF:000009">
        <property type="entry name" value="IQ motif and SEC7 domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1000.11">
        <property type="entry name" value="Arf Nucleotide-binding Site Opener,domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033742">
        <property type="entry name" value="IQSEC_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023394">
        <property type="entry name" value="Sec7_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000904">
        <property type="entry name" value="Sec7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035999">
        <property type="entry name" value="Sec7_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663:SF342">
        <property type="entry name" value="FI21420P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663">
        <property type="entry name" value="GUANYL-NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16453">
        <property type="entry name" value="IQ_SEC7_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01369">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00222">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48425">
        <property type="entry name" value="Sec7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50190">
        <property type="entry name" value="SEC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SEC7" evidence="7">
        <location>
            <begin position="1047"/>
            <end position="1240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="12"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="316"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="543"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="575"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="626"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="907"/>
            <end position="1024"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="39"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="124"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="144"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="172"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="191"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="328"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="376"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="397"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="431"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="578"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="600"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="673"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="725"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="758"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="781"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="974"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1001"/>
            <end position="1024"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8046526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1405" mass="157842" checksum="F658013B03898928" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CKC3</accession>
    <name>A0A9Q1CKC3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Hyalin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_04984</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CKC3"/>
    <dbReference type="OrthoDB" id="6121280at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003410">
        <property type="entry name" value="HYR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24273">
        <property type="entry name" value="FI04643P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24273:SF32">
        <property type="entry name" value="HYALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02494">
        <property type="entry name" value="HYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50825">
        <property type="entry name" value="HYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="HYR" evidence="2">
        <location>
            <begin position="15"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8046343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="144" mass="15522" checksum="56AFFFC07A6B548F" modified="2023-09-13" version="1">MCIVSKKMFSPLFLVDDSPPRVECGPDITRETPMATGGLQVNFEECTATDDSGTAILLSRSSSPGQFFPIGSTEVVYTFTDPTGNTATGSFTITVVGGEKFSTLLFVTKCERNYLPNIVTIIFDSLLKAEGIIAITLVSVYECK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CKF0</accession>
    <name>A0A9Q1CKF0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DnaJ homolog subfamily C member 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HOLleu_05851</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Nanhai2018</strain>
            <tissue evidence="15">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CKF0"/>
    <dbReference type="OrthoDB" id="1690618at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051083">
        <property type="term" value="P:'de novo' cotranslational protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006450">
        <property type="term" value="P:regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000180">
        <property type="entry name" value="DnaJ (Hsp40) homolog, subfamily C, member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.840">
        <property type="entry name" value="Ribosome-associated complex head domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032003">
        <property type="entry name" value="RAC_head"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042569">
        <property type="entry name" value="RAC_head_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054076">
        <property type="entry name" value="ZUO1-like_ZHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044634">
        <property type="entry name" value="Zuotin/DnaJC2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43999">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43999:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23082">
        <property type="entry name" value="Myb_DNA-binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16717">
        <property type="entry name" value="RAC_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21884">
        <property type="entry name" value="ZUO1-like_ZHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="J" evidence="11">
        <location>
            <begin position="92"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="13">
        <location>
            <begin position="553"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="14">
        <location>
            <begin position="557"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="12">
        <location>
            <begin position="557"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="41"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="431"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="531"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="289"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="58"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="431"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="446"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="531"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="551"/>
            <end position="561"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ8046987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="623" mass="72467" checksum="B2FBB45A821FCCEB" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CKM7</accession>
    <name>A0A9Q1CKM7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">UDP-xylose and UDP-N-acetylglucosamine transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_06009</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleotide-sugar transporter family. SLC35B subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CKM7"/>
    <dbReference type="OrthoDB" id="999962at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005462">
        <property type="term" value="F:UDP-N-acetylglucosamine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005464">
        <property type="term" value="F:UDP-xylose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013657">
        <property type="entry name" value="SCL35B1-4/HUT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10778:SF4">
        <property type="entry name" value="NUCLEOTIDE SUGAR TRANSPORTER SLC35B4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10778">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08449">
        <property type="entry name" value="UAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="33"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="68"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="96"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="123"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="158"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="239"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="266"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="293"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8047107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="331" mass="37217" checksum="50DF374EAD09ED5F" modified="2023-09-13" version="1">MSVTAAIAIGLVLIGCCTNVVFLELIIRYDSGAGNLITFGQFLFIALEGFIFTADFGRKKPVIPIAKYATMVAFFFTVSVSNNFCFYFKIPLPLHMIFRAGSLIANMILGVIILKKQYPFSKYLSVLMITFGIFLCTLLSARDVKSDDEDEDNVTYKYVMWLCGIGVLTFALFLSARMGLYQEVLYSSYGKHPREALFYTHALPLAGFLLIGKDIYNHCVILSQSESFEAFGVTIPEQWLYFLGNVVTQYVCIRGVFILTTECPSLVVTLVITLRKFISLIFSILYFKNPFTIGHWIGTFMVFSGVLLFTEAIQKLYSATFAPKKEAVKVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CKW4</accession>
    <name>A0A9Q1CKW4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sulfotransferase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_06120</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CKW4"/>
    <dbReference type="OrthoDB" id="2405944at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48312">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48312:SF1">
        <property type="entry name" value="SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8047186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="311" mass="36964" checksum="5E310F3E1445BF60" modified="2023-09-13" version="1">MDFKQENQQTRIFVWSWPRSLSTVFAKFLSCIDDVQLWHEPYIQSFYSNAMPKVDEADNQPVTKFYLKTHHAMENLHNQPHEFSDSSLLPISLFTYPFIKSKLEEEEPGKKYIFIKDIAPAIMDHYEFLPNQVPTRHTFLIRHPYRFLTSRKRLYMKNTNYQGRPEDFDMHEMSSNIQRRQYERDSMYTLWKYVQESGRDPNPIIIDAEDVSNHPEKVLPQYFAQLGIPFHVKYLTWDSSLDPIKKWKGSKEQVAINYKNGYFGRALDSCCFEGSPQPTPTREDVTPDIIKMAEFILPGYDEMYEKRVKPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CMI8</accession>
    <name>A0A9Q1CMI8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ficolin-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_06712</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CMI8"/>
    <dbReference type="OrthoDB" id="7871457at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00087">
        <property type="entry name" value="FReD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050373">
        <property type="entry name" value="Fibrinogen_C-term_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040941">
        <property type="entry name" value="GGGWT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143">
        <property type="entry name" value="FIBRINOGEN/TENASCIN/ANGIOPOEITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143:SF444">
        <property type="entry name" value="PROTEIN SCABROUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00186">
        <property type="entry name" value="FBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="2">
        <location>
            <begin position="146"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8047661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="362" mass="41280" checksum="34CF53390EE9F1E7" modified="2023-09-13" version="1">MTPVFEKERPTSLSLENPPLTSTQAKPLSATNTLYSNCTTCQDTCELPDGGKMNCNVPHSCVCPQRDGFPTKGEESVPQMDCRCFLQDEGVVITGELHTTPNCSKGCECRANRLVCSTLKCSENTTCDVREGVGCCYCVEGCCHYREMCLHFEDCLDLFNANFTESGIYTIKPLNWPGTPFKVYCNMTDGGGWTVFQRRLDHSVNFYRNWMSYKEGFGSRDHEHWLGNEIIFYLTNQKTYQLRIDFVAPNGNPSYAKYDLFRIANESNNYMLIGVGRYTKPSVTFSRTGYRDNLLYHIYQSFSTYDRDHDSNVKGNCAIEFHSAWWFKSCRHCDLNAANIHWGLLPGGSHNIKYTEMKLRPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CRT1</accession>
    <name>A0A9Q1CRT1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_03317</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CRT1"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8050208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="95" mass="11263" checksum="81B88886A76F0E7D" modified="2023-09-13" version="1">MLLKLQKYQLFVSFKKSSELHVADALKCVVDPLPISRERLKQIEKETHKDEIFLKLHSQIVDESPARKQKLDKELAPYWSCQSELKVEKRNHFTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CRV5</accession>
    <name>A0A9Q1CRV5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_02820</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CRV5"/>
    <dbReference type="OrthoDB" id="10241202at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8049885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="94" mass="11042" checksum="B519C230675FBBDE" modified="2023-09-13" version="1">MFKILLYTFKALNCLAPVYPSDLLKLYQPNRSLRSEQKSLLTKPITRTKLYGNRRFDYASAALWNDLPTDIKNATSVTQFKKYLKTYLFRAAFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CRZ7</accession>
    <name>A0A9Q1CRZ7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CARD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_03722</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CRZ7"/>
    <dbReference type="OrthoDB" id="1357022at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01671">
        <property type="entry name" value="CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001315">
        <property type="entry name" value="CARD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00619">
        <property type="entry name" value="CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00114">
        <property type="entry name" value="CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50209">
        <property type="entry name" value="CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CARD" evidence="1">
        <location>
            <begin position="3"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8050497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="133" mass="15122" checksum="48C42369B31E4360" modified="2023-09-13" version="1">MAMTPEQRRVLDLALPMLRQDLDVYRCLPVLIAENLLSDDDIDVLSAEPVRSLRVIKFIRILQQRGSRAYDVFLNILRQLEGASHLADHITELYESLIETNSLPPARTVNITLSGYDAHFDGDAMLMIFIIVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CSS5</accession>
    <name>A0A9Q1CSS5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Prolyl endopeptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_04060</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CSS5"/>
    <dbReference type="OrthoDB" id="248387at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070012">
        <property type="term" value="F:oligopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000275">
        <property type="entry name" value="Prolyl endopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.120">
        <property type="entry name" value="Prolyl oligopeptidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023302">
        <property type="entry name" value="Pept_S9A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051167">
        <property type="entry name" value="Prolyl_oligopep/macrocyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42881">
        <property type="entry name" value="PROLYL ENDOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42881:SF2">
        <property type="entry name" value="PROLYL ENDOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02897">
        <property type="entry name" value="Peptidase_S9_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50993">
        <property type="entry name" value="Peptidase/esterase 'gauge' domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S9A N-terminal" evidence="1">
        <location>
            <begin position="7"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8050748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="156" mass="18254" checksum="F21C47770751447E" modified="2023-09-13" version="1">MEKFEYPQARRDESIVDDYHGTKVPDPYAWLEDPDGEETKAFVEAQNKITMPYLEKCAYRDIYKKRLTEMWDYPKYGCPFKRGPRYFYHFNTGLQNQSVLYVQDSLDAEARVFIDPNKLSEDGTVAMTRTSFTEDGEYCAYMLSASGSDWNTIKVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1GZI1</accession>
    <name>A0A9Q1GZI1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_29683</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8027675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1GZI1"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8027675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="77" mass="8249" checksum="760F4A478E927C43" modified="2023-09-13" version="1">MAVVAPSIGLGALQFSKELEAKNGLKSRHTMAAVPLTVSANRQCHAFPLFTNFCNSKGGTHIHSLHASIHVLYTCLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H001</accession>
    <name>A0A9Q1H001_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_30045</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8027945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H001"/>
    <dbReference type="OrthoDB" id="2113965at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8027945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="52" mass="5845" checksum="9262A5D8CEA9C432" modified="2023-09-13" version="1">MRYGKRKNPIIFGGGERSSGVTRGQTLKTSLTRYLKKGSSDSSQTWYIDAPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H176</accession>
    <name>A0A9Q1H176_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Multidrug resistance-associated protein 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HOLleu_27970</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Nanhai2018</strain>
            <tissue evidence="15">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8031287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18592">
        <property type="entry name" value="ABC_6TM_MRP5_8_9_D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18599">
        <property type="entry name" value="ABC_6TM_MRP5_8_9_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000012">
        <property type="entry name" value="ATP binding cassette subfamily C member 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000074">
        <property type="entry name" value="Multidrug resistance-associated protein 5 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000015">
        <property type="entry name" value="multidrug resistance-associated protein 5 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002835">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF447">
        <property type="entry name" value="MULTIDRUG RESISTANCE-ASSOCIATED PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
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    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
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        <location>
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            <end position="155"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
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            <begin position="215"/>
            <end position="236"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="248"/>
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    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="327"/>
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    <feature type="transmembrane region" description="Helical" evidence="12">
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            <begin position="355"/>
            <end position="375"/>
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        <location>
            <begin position="437"/>
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    </feature>
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            <end position="483"/>
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    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="953"/>
            <end position="973"/>
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            <begin position="1026"/>
            <end position="1047"/>
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    </feature>
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        <location>
            <begin position="1053"/>
            <end position="1074"/>
        </location>
    </feature>
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        <location>
            <begin position="1144"/>
            <end position="1163"/>
        </location>
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        <location>
            <begin position="218"/>
            <end position="495"/>
        </location>
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        <location>
            <begin position="584"/>
            <end position="810"/>
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            <begin position="904"/>
            <end position="1197"/>
        </location>
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            <begin position="1235"/>
            <end position="1469"/>
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            <dbReference type="ARBA" id="ARBA00022448"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ8031287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1480" mass="164632" checksum="61EB77DCE6D03C82" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H239</accession>
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        <submittedName>
            <fullName evidence="4">Zinc finger protein MSN4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_26615</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome 13"/>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Znf_C2H2_sf"/>
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    </dbReference>
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        <property type="entry name" value="DNA-DIRECTED DNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN CBG10425"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
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    </dbReference>
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        </location>
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        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAJ8030253.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H2V4</accession>
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        <submittedName>
            <fullName evidence="3">E3 SUMO-protein ligase RanBP2</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="ORF">HOLleu_27588</name>
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    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
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            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8031003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome 13"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000018">
        <property type="entry name" value="E3 SUMO-protein ligase RanBP2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000156">
        <property type="entry name" value="Ran_bind_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045255">
        <property type="entry name" value="RanBP1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23138:SF87">
        <property type="entry name" value="E3 SUMO-PROTEIN LIGASE RANBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23138">
        <property type="entry name" value="RAN BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00638">
        <property type="entry name" value="Ran_BP1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00160">
        <property type="entry name" value="RanBD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50196">
        <property type="entry name" value="RANBD1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="200"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="RanBD1" evidence="2">
        <location>
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            <end position="547"/>
        </location>
    </feature>
    <feature type="domain" description="RanBD1" evidence="2">
        <location>
            <begin position="552"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="545"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="77"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="545"/>
            <end position="573"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8031003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="707" mass="80486" checksum="4CDC8010A97742A1" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H4S8</accession>
    <name>A0A9Q1H4S8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_22694</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H4S8"/>
    <dbReference type="OrthoDB" id="6353782at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8035457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="154" mass="16878" checksum="A4CA2874465A4067" modified="2023-09-13" version="1">MRRLDDVITIIINMVVFCTQTSAGGSDIFGCPPSKSFEFGSRGLISCTFPTDLSEVYWYDDVSGRSDIPAIAGYRLSNHEKSGLGVEGGEYDILPNGTLVITNVTLRHEKQYKVLACTTRNCKPSSSLSVLVTGKFEHVFKAIMTLCSIKLLES</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H520</accession>
    <name>A0A9Q1H520_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_23241</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H520"/>
    <dbReference type="OrthoDB" id="421075at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="374"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8033103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="627" mass="73233" checksum="8448D300584101CC" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H691</accession>
    <name>A0A9Q1H691_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_17846</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H691"/>
    <dbReference type="OrthoDB" id="28322at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8037107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="58" mass="6687" checksum="4AFE11C23ADD3E6F" modified="2023-09-13" version="1">MSGFSCHTNYTKRGILFTYPHQKKRILSTVLEDVIQVKRMTFKKKQSPVVLGSRETDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H6H6</accession>
    <name>A0A9Q1H6H6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Zonadhesin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_22034</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H6H6"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051860">
        <property type="entry name" value="Plasmodium_CSP_Invasion"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44826:SF14">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44826">
        <property type="entry name" value="SPORE COAT PROTEIN SP85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="206"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="238"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="266"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="46"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="126"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="140"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="151"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8034979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="303" mass="35798" checksum="4DFB9ADCE2C588C8" modified="2023-09-13" version="1">MLLSIYRPTYRPTYQPTQRPTYRTTYRPTNRPADRPTDRPSDRPTDLPTYLPTDLPTYLPTYLPTYLPTYLPTCLPAYRPTDRPTDRPTDRPTDRPTDRPTDRPTDRPTDRPTDRPTYRPTDRPTNRPTDLPTYRPTDLPTDRPTDRPTDRPTYLPTYRPTDRPTYLPTPPLSIHPSIYPPTHPPTHPSIHPSIYPFIHLSIHPSIYLSIYLSIYLSIYLSIYLSIYLSIYLSIYLSIYLSIYLSIYLSIYLSIYLSIYLSIYLSIYLSIYLSIYLSIYLSIYIYIYIYIYNTQQNNILFQSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H876</accession>
    <name>A0A9Q1H876_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cytochrome P450 4V2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_16982</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H876"/>
    <dbReference type="OrthoDB" id="1470350at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050196">
        <property type="entry name" value="Cytochrome_P450_Monoox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291:SF189">
        <property type="entry name" value="CYTOCHROME P450 4C3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 6" id="KW-0349">Heme</keyword>
    <keyword evidence="5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="5">
        <location>
            <position position="293"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8039312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="354" mass="40620" checksum="309DC5D4FF1924DE" modified="2023-09-13" version="1">MVHWQRITVAYCVETSLSISDSSKVITTRKEEIQKLCNEECIINDVSLEARRHRKLALLDFLIQIQKNDSNFTDDEIQHEVDTFMFGVNDTTSTAVTFTLYLIGRHPKVQRKLQAELRRVFGKYDESHRSWSLVQAIFLRKRPIFQLRIGTDIFVMYAAFRTRAFFYLLLVGNSGSLKILKGKKCSFAGNDRERSVTSEDLQELEYMSCVIKESHRIFPPVPFIGREIEEDISICGSEVPKGVSVFLAVYILHRDPHYFPNPEKFDPDRFLPANSEGRHNCAFIPFSAGPRNCIGQKFATMEQKVLLATILRKLNITSLQTPEELDLTSEIVLRPRNAVNVKLNLRPSVTDEGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H899</accession>
    <name>A0A9Q1H899_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NXPE family member 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_17111</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H899"/>
    <dbReference type="OrthoDB" id="5950832at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057106">
        <property type="entry name" value="NXPE4_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16165">
        <property type="entry name" value="NXPE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16165:SF5">
        <property type="entry name" value="NXPE FAMILY MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24536">
        <property type="entry name" value="NXPE4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NXPE C-terminal" evidence="1">
        <location>
            <begin position="12"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF24536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8039402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="216" mass="25643" checksum="D92FE379ED36F4C4" modified="2023-09-13" version="1">MSTQGYFLNDTWHPFRCKIKTFSVEETLTCLKGTHIYMFGDSTIRQFKEYFQSTFEGFKDISEKRRFPLSPKHLYNSVYNITVKYRSHGLPLKTPCQPMTEIHYVFNELDKLQGGPDVVILISLWAHFGKTTLYLYEKRIEAILDSVQRLLERFPETTVIFKGANTRNNDDSFWAICSSDWVAYRFEKKVAKYTFKKEFEHWVLRRLGSNYCPLFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H9F6</accession>
    <name>A0A9Q1H9F6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_18846</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H9F6"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8037904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="88" mass="9531" checksum="7F721F56AB8CDB34" modified="2023-09-13" version="1">MIPNGVHKHWSLRPGSGDGSPPPRKCLTNRILSEDSQKEPLIFEETLLNVRFHTGGSNGGAGSLTSWISPLTVDPTSAARGVNRVNRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HB24</accession>
    <name>A0A9Q1HB24_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_17094</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 29 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HB24"/>
    <dbReference type="OrthoDB" id="10264956at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008373">
        <property type="term" value="F:sialyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1480.20">
        <property type="entry name" value="Glycosyl transferase family 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001675">
        <property type="entry name" value="Glyco_trans_29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038578">
        <property type="entry name" value="GT29-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23136:SF12">
        <property type="entry name" value="ALPHA-2,6-SIALYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23136">
        <property type="entry name" value="TAX1-BINDING PROTEIN 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00777">
        <property type="entry name" value="Glyco_transf_29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8039390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="318" mass="37624" checksum="F30574F8E87DD43B" modified="2023-09-13" version="1">MLYLYLAYLMEILNVLFLWKDASLFCKKRVHSFFLKVGYNLLLLRMFPLLLLLQVIQHCKKCAIVTSSGHLLGTKAGPEIDSTECVIRMNAAPTVKYEADVGQRTTFRIVGHRNFPRMFDSEPERQSYFVNPKTKSDAIVTAWLYAVNVGRNIETFLSKKYSKKYKDVKFFITSEEQMIWNEKLFYQMLGIKKPKKQLWMSTGWTTMIFALEVCDDIHVYGMTYEEYCKEHPSDRTYYHYFDKLRRACDYFKISENRITTGHKFLTEKLAFANWASLYNITFHYPSWKNHTSSRVSWESPFHKTFLKFQNNTKKADNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HBC6</accession>
    <name>A0A9Q1HBC6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Anaphase-promoting complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_16890</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the cullin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HBC6"/>
    <dbReference type="OrthoDB" id="5581181at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007091">
        <property type="term" value="P:metaphase/anaphase transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070979">
        <property type="term" value="P:protein K11-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.130:FF:000008">
        <property type="entry name" value="anaphase-promoting complex subunit 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1310.10">
        <property type="entry name" value="Cullin Repeats"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.130">
        <property type="entry name" value="Cullin, Chain C, Domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044554">
        <property type="entry name" value="ANAPC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014786">
        <property type="entry name" value="ANAPC2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059120">
        <property type="entry name" value="Cullin-like_AB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016158">
        <property type="entry name" value="Cullin_homology"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036317">
        <property type="entry name" value="Cullin_homology_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057975">
        <property type="entry name" value="TPR_ANAPC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45957">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45957:SF1">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08672">
        <property type="entry name" value="ANAPC2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26557">
        <property type="entry name" value="Cullin_AB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25773">
        <property type="entry name" value="TPR_ANAPC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01013">
        <property type="entry name" value="APC2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00182">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75632">
        <property type="entry name" value="Cullin homology domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50069">
        <property type="entry name" value="CULLIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0498">Mitosis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Cullin family profile" evidence="8">
        <location>
            <begin position="467"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="domain" description="Cullin family profile" evidence="8">
        <location>
            <begin position="813"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="420"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="538"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="543"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="556"/>
            <end position="567"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00016068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8039238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1064" mass="121801" checksum="EB41E9226DA6D376" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HBS2</accession>
    <name>A0A9Q1HBS2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF4806 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_13730</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8039658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HBS2"/>
    <dbReference type="OrthoDB" id="10066031at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032071">
        <property type="entry name" value="DUF4806"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34153:SF2">
        <property type="entry name" value="SI:CH211-262H13.3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34153">
        <property type="entry name" value="SI:CH211-262H13.3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16064">
        <property type="entry name" value="DUF4806"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4806" evidence="1">
        <location>
            <begin position="257"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8039658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="384" mass="42840" checksum="E2E01AD640389402" modified="2023-09-13" version="1">MGKVTVINRFIYPLLWYPGTVLAAPEGVLVRLERAVFKFLWSGKTELVRRKVVYQDLRHGGLGLVHFPSKLRFLLTNNSVPHSGRPSGTYGHMRDTLRMMSTVGDVLQSSAGDLYQAIFDGLAGRVQPSHYALGPEVWRVVHSSAFGGVLEMAFAPALGAQSGRVCHMFWHCGFVALLWEWFHRLADRLTFRNTWAVSQDYALYGLLPPPGNTTFEKRVISLLTEIKADVGHNTALLKTITDAIILPESEEDEWISNFPLHSLDDVTAAEDKLENDAAARKKLVKKMSMIGGSTLDSTVRRILTFLIGSSLATKFNWLGRGHADKKAFSNLLLKDLIFRAISRNPLTKTATLSQVEAIIKMWLKHAADRNGSRKAQNPQTQSES</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HBZ9</accession>
    <name>A0A9Q1HBZ9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_14008</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the GASK family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8039868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HBZ9"/>
    <dbReference type="OrthoDB" id="10011371at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029207">
        <property type="entry name" value="FAM198"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15905:SF1">
        <property type="entry name" value="GOLGI-ASSOCIATED KINASE 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15905">
        <property type="entry name" value="GOLGI-ASSOCIATED KINASE 1B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15051">
        <property type="entry name" value="FAM198"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="158"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="173"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="195"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="213"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8039868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="569" mass="64793" checksum="D48DEC60FC0F4BE5" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HGK1</accession>
    <name>A0A9Q1HGK1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">glutathione transferase</fullName>
            <ecNumber evidence="1">2.5.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_11557</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="1"/>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8044173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HGK1"/>
    <dbReference type="OrthoDB" id="414243at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03039">
        <property type="entry name" value="GST_N_Sigma_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050213">
        <property type="entry name" value="GST_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11571">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11571:SF224">
        <property type="entry name" value="HEMATOPOIETIC PROSTAGLANDIN D SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="4">
        <location>
            <begin position="2"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8044173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="63" mass="7729" checksum="EC5B1A2A6733AB03" modified="2023-09-13" version="1">MPRYKLIYFNARARAEVVRYMMELKDISYEDHRIQFEKWPELRPSKSLLFGRLITFLLYNLTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HGS1</accession>
    <name>A0A9Q1HGS1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transmembrane protein 179B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_08225</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TMEM179 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HGS1"/>
    <dbReference type="OrthoDB" id="6423876at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059010">
        <property type="entry name" value="TMEM179-179B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029776">
        <property type="entry name" value="TMEM179B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31056">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 179B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31056:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 179B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26158">
        <property type="entry name" value="Claudin_TMEM179-179B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="68"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="101"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="183"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00093776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8045255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="227" mass="25324" checksum="BDF51FA12D4BEC72" modified="2023-09-13" version="1">MAAFSWLVSFAETLLLVATGFCGMIVSICVALARNGFDGNCILFSEVKWCNDTALNFTYLGKRGSCHFIIVSEVLIAFYALFYAIYHVCYLVERMKEYHRLMLPAIVANSVCLICIFVSACLLSIGFRRFCKSYVSGFIGTPPDDFSCSTVPSWNQHPKCGEFPEHKDFTSNNYYQLFTTSQGAAWFGFLFWCLATAGLVARRIMYKPSVGDPTTSDKAPITGNIVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HGZ4</accession>
    <name>A0A9Q1HGZ4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Zinc finger protein 99</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_02327</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HGZ4"/>
    <dbReference type="OrthoDB" id="6910977at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000012">
        <property type="entry name" value="RB-associated KRAB zinc finger protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001228">
        <property type="entry name" value="Zinc finger protein 236"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000110">
        <property type="entry name" value="Zinc finger protein-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050717">
        <property type="entry name" value="C2H2-ZF_Transcription_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14196">
        <property type="entry name" value="ODD-SKIPPED - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14196:SF12">
        <property type="entry name" value="ZINC FINGER PROTEIN 208-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="209"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="237"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="265"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="443"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="471"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="499"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="527"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="555"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="583"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="611"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="639"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="323"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="344"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8049542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="669" mass="76565" checksum="047BB6C02C3FE694" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HHQ5</accession>
    <name>A0A9Q1HHQ5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_08357</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HHQ5"/>
    <dbReference type="OrthoDB" id="5953030at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8045361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="128" mass="15046" checksum="131A4119D05B6453" modified="2023-09-13" version="1">MVQNMAIRCLHNLNKYDHITSYISSEHWLTVDKRIKFKLMLIIYKCLNNQGPKYLNDMLMKDFNAVHNLRSNSDTLRLVDPRTTSKSCGDRAFMVGGPRLWNSLPLSLRSVKDTFKFKSRLKTYLFNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HIA7</accession>
    <name>A0A9Q1HIA7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Calcium-responsive transcription factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_09068</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HIA7"/>
    <dbReference type="OrthoDB" id="8907856at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029309">
        <property type="entry name" value="CaRF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47456">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47456:SF1">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15299">
        <property type="entry name" value="ALS2CR8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04434">
        <property type="entry name" value="SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="3">
        <location>
            <begin position="303"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="432"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8045946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="460" mass="53394" checksum="A7A10B5E795E4F30" modified="2023-09-13" version="1">MLGYFKKTLQAGRFGYVHTNGEAESAKELFEKSTSSRFVVLKSNGNFGKKRRYDGSQVVQWDRQHRELVPFIHIGNKIYECHRGKDWKAKMKLERKKARIKETMDNPDAKKMRFHKGTKKAGCTARIVMREVMKFPTYEIDSDTAYKRKVTSSRLKQALKNDPSIGERRIYIQFPRSCDHHGHDLDDSDRDNLILDPRIVARIHELKVNSQVQTIAEMKFLLKEFVTDVLCKDEPLPHKKDRRFFPTVSDIRKVLQKTEKMVNTKSALVIHCKENIPDLHIRIIDETHGKFEVWNKSCPEKVYIVDFCSDGQPSCSCSDWMGSKQPCEHFRAVFHQTSWDLEKLPEEFRGPVLQSSGNEYFVATLTMDPPSETNDLHKNGDDEMVETVIEEIDSSLSDLEDNYETISGGDSLETLRLKCRDQLKRIEDLTHLVKDEAELSQLLKTLHESKRNLEELAEKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HJ42</accession>
    <name>A0A9Q1HJ42_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">mannose-1-phosphate guanylyltransferase</fullName>
            <ecNumber evidence="3">2.7.7.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_05553</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; GDP-alpha-D-mannose from alpha-D-mannose 1-phosphate (GTP route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.13" evidence="3"/>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HJ42"/>
    <dbReference type="OrthoDB" id="1733332at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004475">
        <property type="term" value="F:mannose-1-phosphate guanylyltransferase (GTP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009298">
        <property type="term" value="P:GDP-mannose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06425">
        <property type="entry name" value="M1P_guanylylT_B_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.10.10:FF:000018">
        <property type="entry name" value="Mannose-1-phosphate guanyltransferase beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000013">
        <property type="entry name" value="mannose-1-phosphate guanyltransferase beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056729">
        <property type="entry name" value="GMPPB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045233">
        <property type="entry name" value="GMPPB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018357">
        <property type="entry name" value="Hexapep_transf_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050486">
        <property type="entry name" value="Mannose-1P_guanyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22572">
        <property type="entry name" value="SUGAR-1-PHOSPHATE GUANYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25087">
        <property type="entry name" value="GMPPB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00101">
        <property type="entry name" value="HEXAPEP_TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleotidyl transferase" evidence="8">
        <location>
            <begin position="2"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="Mannose-1-phosphate guanyltransferase C-terminal" evidence="9">
        <location>
            <begin position="250"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF25087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8046768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="359" mass="39952" checksum="29CCA959B79470DA" modified="2023-09-13" version="1">MKALILVGGYGTRLRPLTLSAPKPLVEFCNKPMLLHQVEALAQVGVKEIVLAVSYRAEQLEKELKAQEKKLGIKITMSHEEEPLGTAGPLALAKSILSADSEPFFVLNSDISCEFPFQQMVDFHKSHRQEGTIVVTKVEEPSKYGVVVYDDKGKIERFVEKPSVFVSNKINAGMYIFNPAILDRIELRPTSIEKEIFPVMAKAGQLYAMELSGFWMDVGQPKDFLTGMCMYLSNQKQRFPERLYNGQGVNGNVLVDPTAKIGKNCRIGPNVVIGPDVVIEDGVCIKRSTILKGTTIKSHSWIESSIIGWRCQVGQWVRMENVCVLGEDVIVKDELYINGGRILPHKSIGTSVTEPQIIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HJY5</accession>
    <name>A0A9Q1HJY5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">NUAK family SNF1-like kinase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_06904</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HJY5"/>
    <dbReference type="OrthoDB" id="193931at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050321">
        <property type="term" value="F:tau-protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF93">
        <property type="entry name" value="NUAK FAMILY SNF1-LIKE KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="28"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8047803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="542" mass="60530" checksum="41EFAF1AEA49B0E3" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HK39</accession>
    <name>A0A9Q1HK39_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Alpha-N-acetylneuraminide alpha-2,8-sialyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_00106</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 29 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8047968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HK39"/>
    <dbReference type="OrthoDB" id="10264956at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003828">
        <property type="term" value="F:alpha-N-acetylneuraminate alpha-2,8-sialyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006491">
        <property type="term" value="P:N-glycan processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009311">
        <property type="term" value="P:oligosaccharide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1480.20">
        <property type="entry name" value="Glycosyl transferase family 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001675">
        <property type="entry name" value="Glyco_trans_29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050943">
        <property type="entry name" value="Glycosyltr_29_Sialyltrsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038578">
        <property type="entry name" value="GT29-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11987">
        <property type="entry name" value="ALPHA-2,8-SIALYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11987:SF36">
        <property type="entry name" value="SIA-ALPHA-2,3-GAL-BETA-1,4-GLCNAC-R:ALPHA 2,8-SIALYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00777">
        <property type="entry name" value="Glyco_transf_29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8047968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="75" mass="9047" checksum="90FD6F88A73B56C0" modified="2023-09-13" version="1">MLTIAETFCKDITLYGFYPYQKDSSGAHILHHYYEPNLKDFHTDAHDFEEEHTLFKSLHKRKFLRLVVERCETKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HLY5</accession>
    <name>A0A9Q1HLY5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_04588</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8051135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HLY5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8051135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="64" mass="7288" checksum="682456DFDBF19AF4" modified="2023-09-13" version="1">MAYRQTPSEFNVGYGRGVPCYLYSMYCLVNPLQEPSKLNLRFDHFTHLVELKSSASSMLTTSHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0Y8J9</accession>
    <name>A0A9Q0Y8J9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_44834</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010001312">
        <property type="protein sequence ID" value="KAJ8017623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0Y8J9"/>
    <dbReference type="OrthoDB" id="2286242at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8017623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="68" mass="7689" checksum="1156D15FC1EA68C9" modified="2023-09-13" version="1">MRSQDSLIGDRIICGIPENALKERQQREKDLTLSKAVQICRVAETTRSQMKELQTDDVVSVHAVYSAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0Y9V7</accession>
    <name>A0A9Q0Y9V7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_43012</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000184">
        <property type="protein sequence ID" value="KAJ8018802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0Y9V7"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8018802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="110" mass="13000" checksum="384831F7BF3A5BBF" modified="2023-09-13" version="1">MMSHCPTVLTNFTLALIVTTIVNRHQHCAMMISLHSWSLTMMLDIPFISWMHTRRQDPMVLLHVYLNYVTPRWQRFSQKSLTGHFVSALSHHVLRKLLIYPFLRQIPFPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YA09</accession>
    <name>A0A9Q0YA09_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_42762</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000131">
        <property type="protein sequence ID" value="KAJ8018952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YA09"/>
    <dbReference type="OrthoDB" id="10068277at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="202"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="202"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="226"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8018952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="244" mass="28119" checksum="6AB859B2B25DF939" modified="2023-09-13" version="1">MVLMPQDVARVLNLSSQDKSQLRLNQLDGDMKTTLEREDLSLYEKVELYNQALQRYLDADKQMTSKPIGIKITNTPSDKQKGKDDNDNAAPTPNEAPAMKSDLLLANFPKSLKSKAKLLLERISDHRQSEQQPVIDWNAKGELLYQGETIHGSNLTDLILDVMHSRKDFNPIGWQQFIHGLSKLNFPEAYVGNLMRRQTMHQIREKGPRRDFSKLLPTPPRDERPKRKGNGKSRKRQKITWESF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YDP2</accession>
    <name>A0A9Q0YDP2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_44090</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000631">
        <property type="protein sequence ID" value="KAJ8018102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YDP2"/>
    <dbReference type="OrthoDB" id="10072345at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051532">
        <property type="entry name" value="Ester_Hydrolysis_Enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013830">
        <property type="entry name" value="SGNH_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30383">
        <property type="entry name" value="THIOESTERASE 1/PROTEASE 1/LYSOPHOSPHOLIPASE L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13472">
        <property type="entry name" value="Lipase_GDSL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCHC-type" evidence="1">
        <location>
            <begin position="239"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="1">
        <location>
            <begin position="260"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8018102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="277" mass="31466" checksum="FED5A29828646B9A" modified="2023-09-13" version="1">MQSLNETYSSTPKVLIAGDSILRDFSKEDFEDNVTVLCLRGGTVSDLQQKLDESDITSGINNVILHIGTNDFANNTPVPEITGQFEALITAIQIKNPTPTKVCISGPCPRLDIHCDTVIELSNSLKELAEKSDCNFIDNVKSFSYGDGEVVKSLFYDDVHLSQSGSDKSKAKFTSIGPVCLRDKRRFNRQRKAYNERAPHLTYGSYRPSYSYQTRRQNPRRYNTYVDWNKQSASDYARGCYKCGERNHNETTCRHPQPLVCNNCRRPGHKSRFCRQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-06-18" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YFV8</accession>
    <name>A0A9Q0YFV8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Protein dispatched-like 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_39340</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="429851at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00104">
        <property type="entry name" value="KAZAL_FS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1640.10">
        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042480">
        <property type="entry name" value="DISP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053958">
        <property type="entry name" value="HMGCR/SNAP/NPC1-like_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036058">
        <property type="entry name" value="Kazal_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004869">
        <property type="entry name" value="MMPL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000731">
        <property type="entry name" value="SSD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46687">
        <property type="entry name" value="PROTEIN DISPATCHED HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46687:SF1">
        <property type="entry name" value="PROTEIN DISPATCHED HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07648">
        <property type="entry name" value="Kazal_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03176">
        <property type="entry name" value="MMPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12349">
        <property type="entry name" value="Sterol-sensing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00280">
        <property type="entry name" value="KAZAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100895">
        <property type="entry name" value="Kazal-type serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82866">
        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51465">
        <property type="entry name" value="KAZAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50156">
        <property type="entry name" value="SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="72"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="409"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="431"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="462"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="504"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="536"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="703"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1126"/>
            <end position="1144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1151"/>
            <end position="1172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1184"/>
            <end position="1205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1226"/>
            <end position="1256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1262"/>
            <end position="1284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1296"/>
            <end position="1318"/>
        </location>
    </feature>
    <feature type="domain" description="SSD" evidence="7">
        <location>
            <begin position="432"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="domain" description="Kazal-like" evidence="8">
        <location>
            <begin position="839"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="17"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="770"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="26"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="780"/>
            <end position="804"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8021983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1334" mass="147553" checksum="0193CBFD89C3C750" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YGN7</accession>
    <name>A0A9Q0YGN7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_39560</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8022150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YGN7"/>
    <dbReference type="OrthoDB" id="6361178at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8022150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="117" mass="12981" checksum="A3E0EC2DA15E3093" modified="2023-09-13" version="1">MPHIERKNPIVFGRGQRSPGVTGGQNLKTLLTQYLEMRYGKRKNPIIFGGGQRSSGVTGGQTLKTSLTRYLKMPHIERKNPIVFGGGQRSPGVTRGQNLKTLLTQYLEVGSLDEFHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YHU2</accession>
    <name>A0A9Q0YHU2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_40088</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YHU2"/>
    <dbReference type="OrthoDB" id="6155528at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8020487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="112" mass="12962" checksum="358CA91A84DC3C31" modified="2023-09-13" version="1">MFFFGITVQHPTLLHMFLQQNFCLVVPSHVRVKLPQVEPEPTNDVVRATDRQSKTKMKHYAEKKHSFKQSPIRVGDDSVLVRQNKINKLFPPYDPKPFKFISDKGSLITARA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YJN2</accession>
    <name>A0A9Q0YJN2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Amine sulfotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_36199</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sulfotransferase 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YJN2"/>
    <dbReference type="OrthoDB" id="205623at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11783">
        <property type="entry name" value="SULFOTRANSFERASE SULT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sulfotransferase" evidence="3">
        <location>
            <begin position="42"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8023692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="321" mass="37332" checksum="7407E2211392E60B" modified="2023-09-13" version="1">MDRNKMANFEQLVIFNKNGFPLPANTNEDFMKELYDMEVREDDIFVVTYSKSGAHWMQEIVHLILNEGHVEKLNASHRQIVIEVANCRSFTPETVSASGPVLRKVKSMPSPRVVTTHVPYSLLPKQLQENPCKLIYIYRHPKDVIVSNYHFSSRILEMCTGERVLHSAEQFANFFDLSFAAKFQYGSWFDHVNEYYKHKDDESFLSISFEAMKMDLPTVERKIASFIGRQLDDAAVCRVVEGASFESMQTSFKRDQAKNKALGENVVDTEIFVNKGEMRQWKHYFTAEQNHRFDQLFSEKMQHCHWTKPYALHCSNESSTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YLT4</accession>
    <name>A0A9Q0YLT4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">N-acetylgalactosaminide beta-1,3-galactosyltransferase</fullName>
            <ecNumber evidence="4">2.4.1.122</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_34819</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase 31 family. Beta3-Gal-T subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.122" evidence="4"/>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YLT4"/>
    <dbReference type="OrthoDB" id="414175at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016263">
        <property type="term" value="F:glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026050">
        <property type="entry name" value="C1GALT1/C1GALT1_chp1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003378">
        <property type="entry name" value="Fringe-like_glycosylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23033">
        <property type="entry name" value="BETA1,3-GALACTOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23033:SF14">
        <property type="entry name" value="GLYCOPROTEIN-N-ACETYLGALACTOSAMINE 3-BETA-GALACTOSYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02434">
        <property type="entry name" value="Fringe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Fringe-like glycosyltransferase" evidence="12">
        <location>
            <begin position="25"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8024803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="268" mass="31297" checksum="26B877CACBB9251B" modified="2023-09-13" version="1">MSQKIRILCWIMTHPGNLQTKAIHVNATWVKRCNTVLFMSSENTNFPTIAVVNHEGRDHLWRKTRGAFEYISKYYLDKADWFLKADDDTYVIMENVRKLVSLYDPEKPIFFGRRFKSFDIEFVSGGAGYVLSRKAVKLLTEDAFKDTALCKSATHVGYPEDVEIGRCLKNVGVETGDSRTDIYETFHPFIPEHMLVPSSLLKKDQWYYSLNFYPVQKGPKCCSDYSISFHYVTPDMMYWLEYMVYQMRPFGFGYHTCPTNIRTMIEPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YMX6</accession>
    <name>A0A9Q0YMX6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_39093</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YMX6"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8021796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="67" mass="7934" checksum="C65998FC94F4E102" modified="2023-09-13" version="1">MDKLCFIIVIVDEPFMCGEWITMQQLGPADLNVSSNVKSLWEIHKRLKNFLRINVYHRREGDNNPLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YNU9</accession>
    <name>A0A9Q0YNU9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Lactadherin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_37412</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YNU9"/>
    <dbReference type="OrthoDB" id="5945585at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="CDD" id="cd00057">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000016">
        <property type="entry name" value="Contactin-associated protein-like 4 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000421">
        <property type="entry name" value="FA58C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24543">
        <property type="entry name" value="MULTICOPPER OXIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00754">
        <property type="entry name" value="F5_F8_type_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00231">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01285">
        <property type="entry name" value="FA58C_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50022">
        <property type="entry name" value="FA58C_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040398379" evidence="1">
        <location>
            <begin position="24"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="F5/8 type C" evidence="2">
        <location>
            <begin position="37"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="F5/8 type C" evidence="2">
        <location>
            <begin position="196"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8022501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="344" mass="37779" checksum="2171225F742645AC" modified="2023-09-13" version="1">MMKILLTCCILLVTSVTVSKTDCGPYCSTPPQPGTCCQRLGLNVNRDADVVLSASTEYNSHHGVQRAALFEIDEPGTGIAGWAAATNDGNQWIQADFGSPRQVTGVLLQGRGLGCCNQWVTSFRVLFSDDGEVFYSTEPEKGSLTIENRERHQYTDLKKMMKILLTCCILLVTSVTVSKTDCGPYCSTPPEPGTCCQRLGLNVNRDADVVLSASTEYNSQHGVQRAALFEIDEPGTGIAGWAAATNDVNQWIQVDFGSPRQVSGVLLQGRGLGCCNQWVTSFRVLFSDDGEVFYSTAVIEGTFDRHTVARRFFDRPIVARYFRINPVTWFGHITLRFDLLGCDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YPX3</accession>
    <name>A0A9Q0YPX3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Thioredoxin domain-containing protein 15</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_38183</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8023101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YPX3"/>
    <dbReference type="OrthoDB" id="1899781at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042418">
        <property type="entry name" value="TXNDC15"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14684">
        <property type="entry name" value="THIOREDOXIN DOMAIN-CONTAINING PROTEIN 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14684:SF2">
        <property type="entry name" value="THIOREDOXIN DOMAIN-CONTAINING PROTEIN 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040235784" evidence="1">
        <location>
            <begin position="37"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="2">
        <location>
            <begin position="150"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8023101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="370" mass="42670" checksum="B892BBEF9F4AC650" modified="2023-09-13" version="1">MCIRKNRFVKVMRFNFLMPFQSLLIALILMPSLVIAADDDLMTFVDDVQEDEHTVIIDDSNTSNKSPLPEIHLPLPPKEADIEYVIKLVERKKWQRRTYQALERKTVCLYTYHDVVFRVWEDTTCHIFLPQRDSEGSSSIREIDITNQTSKYSYPCSPRTVLENARAGVQMVNSSEFLRILSQDPETDEEGAAKQHCVVVLFYSKFCPFSMKMAPDFNAFGRAFLGVDIMAVDVSDTSGFSTRYGIVAVPDILLFHRSKPVLRFNFTERTYDNFVAFLQNNTGLEADPDIQVSAEDLEGPLSSTLVQHPDYLLWFSNTFVIFFAICILKQRLGDSILQRVREYLDYARQVWRTGELYEQVGVHDNEDEFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YQ45</accession>
    <name>A0A9Q0YQ45_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Deleted in malignant brain tumors 1 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_31236</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YQ45"/>
    <dbReference type="OrthoDB" id="536948at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000001">
        <property type="entry name" value="Lysyl oxidase 4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000005">
        <property type="entry name" value="Neurotrypsin isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000006">
        <property type="entry name" value="neurotrypsin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000011">
        <property type="entry name" value="Scavenger receptor class A member 5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48071:SF18">
        <property type="entry name" value="DELETED IN MALIGNANT BRAIN TUMORS 1 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48071">
        <property type="entry name" value="SRCR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00530">
        <property type="entry name" value="SRCR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00258">
        <property type="entry name" value="SPERACTRCPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00420">
        <property type="entry name" value="SRCR_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040304034" evidence="8">
        <location>
            <begin position="20"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="9">
        <location>
            <begin position="21"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="9">
        <location>
            <begin position="128"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="9">
        <location>
            <begin position="243"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="9">
        <location>
            <begin position="350"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="9">
        <location>
            <begin position="460"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="87"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="196"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="309"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="421"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="528"/>
            <end position="538"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8026424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="562" mass="61080" checksum="E8CB994E32BF6229" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YQ65</accession>
    <name>A0A9Q0YQ65_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_35591</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YQ65"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040175749" evidence="1">
        <location>
            <begin position="22"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8023236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="60" mass="6876" checksum="F762775465821451" modified="2023-09-13" version="1">MGLGTLMMTSYLLFAFAVTMTQNTICYCQTTIPSYPWSSTRLYNSVDIQRGMKMMQDFSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YQA0</accession>
    <name>A0A9Q0YQA0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">5-hydroxytryptamine receptor 3A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_31465</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YQA0"/>
    <dbReference type="OrthoDB" id="5975154at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005216">
        <property type="term" value="F:monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19051">
        <property type="entry name" value="LGIC_TM_cation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0675">Receptor</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="90"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="127"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="2">
        <location>
            <begin position="65"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8026587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="177" mass="19621" checksum="516D28BB384C1C3D" modified="2023-09-13" version="1">MYLTAEQPDTPSSVDSFEWNLLNTTIWNYVFPYNSSIYSGSSSWYSGVYLCVYLQRDPNYYISTLIIPSTLMCIMAFVTFLAPPDSGERISLGVSMVLGLTVFQLLISNTLPTSSKTLPVLSGYLTVNFILACLAVPFSLFNINIAYSIGKLTILKKTMDQNVAPCAFAIFSLCVHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1B9L9</accession>
    <name>A0A9Q1B9L9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Zinc finger MYM-type protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_43704</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000423">
        <property type="protein sequence ID" value="KAJ8018350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1B9L9"/>
    <dbReference type="OrthoDB" id="10063284at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008906">
        <property type="entry name" value="HATC_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45749">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45749:SF21">
        <property type="entry name" value="DUF4371 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05699">
        <property type="entry name" value="Dimer_Tnp_hAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HAT C-terminal dimerisation" evidence="2">
        <location>
            <begin position="652"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="107"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8018350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="732" mass="83777" checksum="FDE98AEB89153843" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BB47</accession>
    <name>A0A9Q1BB47_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">HOLleu_38596</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">Nanhai2018</strain>
            <tissue evidence="20">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Peroxisome membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the pex2/pex10/pex12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5"/>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BB47"/>
    <dbReference type="OrthoDB" id="6270329at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016558">
        <property type="term" value="P:protein import into peroxisome matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16527">
        <property type="entry name" value="RING-HC_PEX10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025654">
        <property type="entry name" value="PEX2/10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006845">
        <property type="entry name" value="Pex_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23350">
        <property type="entry name" value="PEROXISOME ASSEMBLY PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23350:SF0">
        <property type="entry name" value="PEROXISOME BIOGENESIS FACTOR 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04757">
        <property type="entry name" value="Pex2_Pex12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="7" id="KW-0962">Peroxisome biogenesis</keyword>
    <keyword evidence="14" id="KW-0653">Protein transport</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <keyword evidence="12" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <keyword evidence="11 18" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="19">
        <location>
            <begin position="449"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAJ8021411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="504" mass="57243" checksum="63F20B35CB95FDBA" modified="2023-09-13" version="1">MATNIASRLSERLTDCQNYSLALDESTDISDTAQLAIFVRGVNQNFKVGEELLDLRPIKGTIKGKDIFNEVKGALDKFKVPNEKLVGVTTDGAPALTGKHGGFVTLMKNALGQEEICTLSNTWNKPDASVNHLKYEEMITGLGEEIDRRFQDFGGHIAEMRIFADPFTCDVNAAPDDYQLELIEVQNDEGLKREFTDLHGDIATFCKIMARFESAGQAEILRSSQKDQFYISQLRSKAADVFQSWAGSRAWIKWRAELDLVTDCLYFGLTTLSGLQTLGEEYVNIIQVDHTKRAVPSLQRRGFLIFSHVIFPYLLDKFLSKLTYQLDADLPIPGLSPEFRERLKSVLPVIRHVVLLGHRAHMAFFYIRGIFYHVAKRASGIQYLMVRRGLTSWTLQPSFHILGAVSLMQLILSFIWQLYQWKKYHADKTHEPSSPEIQDDMTEVASLRCSLCLGKRKHSTATPCGHLFCWYCIMEWCATKPECPLCREPIQPSRLIFLQNYGPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BB96</accession>
    <name>A0A9Q1BB96_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">THAP-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_42591</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000096">
        <property type="protein sequence ID" value="KAJ8019068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BB96"/>
    <dbReference type="OrthoDB" id="7331812at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.210.20">
        <property type="entry name" value="THAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027806">
        <property type="entry name" value="HARBI1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006612">
        <property type="entry name" value="THAP_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038441">
        <property type="entry name" value="THAP_Znf_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23080">
        <property type="entry name" value="THAP DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23080:SF63">
        <property type="entry name" value="TICK TRANSPOSON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13359">
        <property type="entry name" value="DDE_Tnp_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05485">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="THAP-type" evidence="6">
        <location>
            <begin position="18"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="DDE Tnp4" evidence="7">
        <location>
            <begin position="73"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8019068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="206" mass="23155" checksum="CCB05282FF1524BE" modified="2023-09-13" version="1">MGFVGRPKGRVHHKGHVEKLQFHRCPEKHCKTWSKLLGYKNYSVTKNTMVCSNHFRHGQPFPADAHPSLYLKAEGAASDKEMVKLSGLIEKLEKVDILMADRGFNIHKLLLHKEVKLIIPPFKRTTRSTDQFTLSEGKSTKIVANSRIHVERVIGRLKEFMFLQGPIPLTFVDLAEAALHVCAALVNLQPALVSLPQCREGAFGDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BBE9</accession>
    <name>A0A9Q1BBE9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_38993</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BBE9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8021711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="87" mass="9773" checksum="D96DEE0E49430DFB" modified="2023-09-13" version="1">MGRSTTNSLVVCSTRQVVFLNHLPASQPPLDLSLHSLPKVPSKDTETNHYSQTNYRKPPLDFLYKVTRRSSRKHILNAVSPTQGGEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BBX1</accession>
    <name>A0A9Q1BBX1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transposase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_42927</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000165">
        <property type="protein sequence ID" value="KAJ8018857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BBX1"/>
    <dbReference type="OrthoDB" id="8007085at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33053">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33053:SF24">
        <property type="entry name" value="TRANSPOSASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8018857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="681" mass="77315" checksum="659332F56490EA9F" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BC77</accession>
    <name>A0A9Q1BC77_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">S-crystallin SL11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_37249</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BC77"/>
    <dbReference type="OrthoDB" id="414243at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03192">
        <property type="entry name" value="GST_C_Sigma_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03039">
        <property type="entry name" value="GST_N_Sigma_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000030">
        <property type="entry name" value="Glutathione S-transferase S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050213">
        <property type="entry name" value="GST_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11571">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11571:SF150">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14497">
        <property type="entry name" value="GST_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="2">
        <location>
            <begin position="61"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8022371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="185" mass="21079" checksum="256ECEA225ED8629" modified="2023-09-13" version="1">MFALAGVEYEDHRIKNDEWPQVKKDYPLGVLPVLEVNGEQIPQSSAINHFLASEFGFLGSSHLEAAKINVVYEVLVDSLYEARKIVEAEDPKIKEQLKTKFYDVILENHSKFLVEILEKNNGGNGFFVGDKITLADIFFLAVRKDILTYVDKDDRLHKQPKLQALADRIAAVPQIAEWIKNRPQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BCW7</accession>
    <name>A0A9Q1BCW7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_39337</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BCW7"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040230663" description="Ig-like domain-containing protein" evidence="2">
        <location>
            <begin position="20"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="377"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8021980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="479" mass="53827" checksum="9ED2568551E2A954" modified="2023-09-13" version="1">MHIFGWFALILLNCQATAGVDDGGLQLFLRNTIGRYRHGNYYYALKEQKVDLICRHTQYRKKLSMNKGVRPESSLLIVNGGELVSSTPGKYSVERQRRRQTLTIQTPDEPDISPYVCWSFEKAGDSNARILFNYTYLHVMEQSDTINFCDLVSGIDGVFFTGDDVVLKCPIGNNLKVGFQPRGRVGSITVHNQACVPMDPPEFCLLSIRNIGRTWDGSFVSCTKHTEGVEEDLCQHIPQITVFDDLTVFVDLLEWNNTDGYAIVACRSIPSVLSPIEWNVVGKNVTIEGTERLNQMKINLRNYSENTVTSFEVSCRLFIGSRSSSKSVSFNTTSITSKLMPITAKESSIKHVTSSVPSDVKELPSTPNVTPDGKTPLILALSIGCTTTILIILSIILVLVFRTKRCLKHKKKSLRVSNIYLSPDQVLATKESTREKFTEMDTSKPTDDNDYIYADTERTLRVIDYTIDDTVLYQNVAHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BDE8</accession>
    <name>A0A9Q1BDE8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_36106</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BDE8"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8023616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="99" mass="10836" checksum="CAC0B50976A005E9" modified="2023-09-13" version="1">MVILLTVCDKVCSNAFHFVSAAERDMNHVGRPNCLIIAKRSFCALCQYGGEPLSCLNILKPSSRCSYVSKVPGSFKSPADGKVGNLHLRRQKDTLCCIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BDH7</accession>
    <name>A0A9Q1BDH7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_38613</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BDH7"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040469505" evidence="1">
        <location>
            <begin position="26"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8021423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="110" mass="12120" checksum="44FCC6F5CB037959" modified="2023-09-13" version="1">MTSTGRSILCIAIAAAFIVFTEAEAAHPGSIKLETEWTEVRDLLYEAQVTLCKLQRRLSVVISDGVNVLPLASESESDLLMTRGNGHLTALEPLEASKRARRCSFWKILC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BE23</accession>
    <name>A0A9Q1BE23_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_35540</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BE23"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8023210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="135" mass="14985" checksum="102707A830B26337" modified="2023-09-13" version="1">MPHIKRKNPIVFGGGQRSPGVTQGQTLKTLLTQYLEVGSLDEFHTLYVPHTKRKNPIVFGGGQRSPGVIGGQTLKTLLTQYLEMPHTKRKNPIVFGGDRRSPGVTGGQTLKTLLTQYLDVGSLDEFHTLYIDVLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BEG4</accession>
    <name>A0A9Q1BEG4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_38891</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BEG4"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8021632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="229" mass="26467" checksum="EA634818D9D27293" modified="2023-09-13" version="1">MTPEKPKRSDLLKSMRDLKRKMRLRYNFQNKESGSISKFRLPSTWMPRPAYSNHLENYLEATLTEIASLPIRNAKSNIKKAEMFALKAIRNDRSLIVKPFDKGRGIAILNRSDYRAEIERQLQSHHYERVPSDITPETVQIVKGTLQDLFHRDEIDEQTFKYLNPQNHKIRTPVIYVLPKIHKAPPANSKFAGRPIISGNGSPTEKNIGICGLFFASHCYATKHLCERH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BEG6</accession>
    <name>A0A9Q1BEG6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_36140</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BEG6"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8023645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="76" mass="8922" checksum="A710B7E11DBC4632" modified="2023-09-13" version="1">MESSREFNFNAAFLELSRSENCLRYVPITTTVCSRSFYLVVIRSWNQFLRYSTLQVYYCTIVNVHALPALGPDQKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BGL3</accession>
    <name>A0A9Q1BGL3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sulfotransferase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_34328</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BGL3"/>
    <dbReference type="OrthoDB" id="2405944at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48312">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48312:SF1">
        <property type="entry name" value="SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8024424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="311" mass="36215" checksum="6102D6A94430A020" modified="2023-09-13" version="1">MFSRRMNGSNQGQIPRIFLWCSPRVNSTVFEKCMSFVDGVQIWHEPYMVCRQLELLTNPMLMPPYLLQKFEEAQNEVDGGKHEFHGAKLEPIDSFSQSWVRGRLEAGERDKKFIFIKDMAAGIVDNLEKLPKGVPCRHTFLIRHPHGYLNSIRKYVMNIYGYTGDPNLFDVTQDNPILHKKNPRDSIHFLWKTVCSKNMDKEPLIIDADDLINYPHKILPKYFEALGIPYDPKYLKWDSDDSVVKKWKGAMEHVLWQKRNGVTSRALASTEFVPGQSSAIPRNELSPDIDQFVDFLMVGYKEMYKKRIKPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BKL5</accession>
    <name>A0A9Q1BKL5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_30398</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BKL5"/>
    <dbReference type="OrthoDB" id="5987693at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="553"/>
            <end position="624"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8028219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="733" mass="81938" checksum="C27B0CEAF20D8722" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BL14</accession>
    <name>A0A9Q1BL14_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_30742</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BL14"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8028491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="86" mass="10051" checksum="5E10EBF2315DC2DE" modified="2023-09-13" version="1">MDSILIFIQVVIWGGFSARDVICGPMRTVLEHSERPNFYAMTVMSKLFIECVWVEWLVWGWGKRKGLPILISLIKSLMFFKKQKSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BM71</accession>
    <name>A0A9Q1BM71_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA polymerase subunit gamma-1</fullName>
            <ecNumber evidence="4">2.7.7.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Mitochondrial DNA polymerase catalytic subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HOLleu_28313</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Nanhai2018</strain>
            <tissue evidence="18">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
            <location evidence="2">Mitochondrion nucleoid</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DNA polymerase type-A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="4"/>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BM71"/>
    <dbReference type="OrthoDB" id="5588663at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005760">
        <property type="term" value="C:gamma DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042645">
        <property type="term" value="C:mitochondrial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006264">
        <property type="term" value="P:mitochondrial DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000024">
        <property type="entry name" value="DNA polymerase gamma, catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.390:FF:000002">
        <property type="entry name" value="DNA polymerase gamma, catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.390:FF:000004">
        <property type="entry name" value="DNA polymerase subunit gamma-1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019760">
        <property type="entry name" value="DNA-dir_DNA_pol_A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002297">
        <property type="entry name" value="DNA-dir_DNA_pol_A_mt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001098">
        <property type="entry name" value="DNA-dir_DNA_pol_A_palm_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041336">
        <property type="entry name" value="DNApol_Exo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10267">
        <property type="entry name" value="DNA POLYMERASE SUBUNIT GAMMA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10267:SF0">
        <property type="entry name" value="DNA POLYMERASE SUBUNIT GAMMA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18136">
        <property type="entry name" value="DNApol_Exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00867">
        <property type="entry name" value="DNAPOLG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00482">
        <property type="entry name" value="POLAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00447">
        <property type="entry name" value="DNA_POLYMERASE_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0235">DNA replication</keyword>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1135">Mitochondrion nucleoid</keyword>
    <keyword evidence="7" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA-directed DNA polymerase family A palm" evidence="17">
        <location>
            <begin position="841"/>
            <end position="1043"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="310"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="492"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="624"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="676"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="422"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="513"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="527"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="635"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="676"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="687"/>
            <end position="698"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00031966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ8029029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1050" mass="120625" checksum="C8A447A2C038A20B" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BMI7</accession>
    <name>A0A9Q1BMI7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Alpha-methylacyl-CoA racemase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_28704</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CoA-transferase III family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BMI7"/>
    <dbReference type="OrthoDB" id="16747at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008111">
        <property type="term" value="F:alpha-methylacyl-CoA racemase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008206">
        <property type="term" value="P:bile acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1540.10:FF:000004">
        <property type="entry name" value="Probable alpha-methylacyl-CoA racemase mcr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10540">
        <property type="entry name" value="Crotonobetainyl-coa:carnitine coa-transferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1540.10">
        <property type="entry name" value="formyl-coa transferase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050509">
        <property type="entry name" value="CoA-transferase_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003673">
        <property type="entry name" value="CoA-Trfase_fam_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044855">
        <property type="entry name" value="CoA-Trfase_III_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023606">
        <property type="entry name" value="CoA-Trfase_III_dom_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48228:SF5">
        <property type="entry name" value="ALPHA-METHYLACYL-COA RACEMASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48228">
        <property type="entry name" value="SUCCINYL-COA--D-CITRAMALATE COA-TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02515">
        <property type="entry name" value="CoA_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89796">
        <property type="entry name" value="CoA-transferase family III (CaiB/BaiF)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="299"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="302"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="334"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8029333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="382" mass="41295" checksum="76D1B56DB39A35EF" modified="2023-09-13" version="1">MALKGVKVIELAGLAPAPFAGMVLADFGAQVIRVDRATQLYSMDRLARGKQSIALNWKKPEGLNVLRKLCVSADVVIEPFRPGVMEKMGLGPQILLKENPRLIYARMTGFGQSGCYKNMAGHDINYIATSGVLAQLGRKGEKPLAPINLLGDFAGGGMVCALGICMALYERAMSGKGQVIDANMVEGSAYISAFTFMTKGIGLVGGERGTNMLDSGAFFYDTYKTLDGKYMAVGALEPQFYAELLKGLGLNPEELPDQLNREEWPAMKKMVETIFASKTREEWTKIFNGKDACVTPVLTPDEAPHHPHNKENKAFLANDDGDLEPVPAPRLSRTPASPRTMAQPSVGQDTETILSELGYSREEMQQLAQAGAVGGVTSQSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BNH8</accession>
    <name>A0A9Q1BNH8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="21">Proprotein convertase subtilisin/kexin type 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">HOLleu_29145</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">Nanhai2018</strain>
            <tissue evidence="21">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16 17">Belongs to the peptidase S8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="300641at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008038">
        <property type="term" value="P:neuron recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016486">
        <property type="term" value="P:peptide hormone processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="CDD" id="cd04059">
        <property type="entry name" value="Peptidases_S8_Protein_convertases_Kexins_Furin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000001">
        <property type="entry name" value="Furin 2, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000006">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.850:FF:000001">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.220.10">
        <property type="entry name" value="Hormone Receptor, Insulin-like Growth Factor Receptor 1, Chain A, domain 2"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.850">
        <property type="entry name" value="Peptidase S8, pro-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032778">
        <property type="entry name" value="GF_recep_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034182">
        <property type="entry name" value="Kexin/furin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002884">
        <property type="entry name" value="P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043601">
        <property type="entry name" value="Rspo_Fu-CRD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032815">
        <property type="entry name" value="S8_pro-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038466">
        <property type="entry name" value="S8_pro-domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42884:SF3">
        <property type="entry name" value="FURIN-LIKE PROTEASE 1, ISOFORMS 1_1-X_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42884">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15913">
        <property type="entry name" value="Furin-like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14843">
        <property type="entry name" value="GF_recep_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01483">
        <property type="entry name" value="P_proprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16470">
        <property type="entry name" value="S8_pro-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SMART" id="SM01411">
        <property type="entry name" value="Ephrin_rec_like"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="23"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51829">
        <property type="entry name" value="P_HOMO_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 16 17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 16 17" id="KW-0645">Protease</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="10 16 17" id="KW-0720">Serine protease</keyword>
    <keyword evidence="7 19" id="KW-0732">Signal</keyword>
    <keyword evidence="18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="12" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040279194" evidence="19">
        <location>
            <begin position="19"/>
            <end position="1869"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1782"/>
            <end position="1805"/>
        </location>
    </feature>
    <feature type="domain" description="P/Homo B" evidence="20">
        <location>
            <begin position="440"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="15 16">
        <location>
            <position position="151"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="15 16">
        <location>
            <position position="192"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="15 16">
        <location>
            <position position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAJ8029689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1869" mass="205501" checksum="63F964C6B8186B4D" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BQ51</accession>
    <name>A0A9Q1BQ51_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Alkaline ceramidase</fullName>
            <ecNumber evidence="9">3.5.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_27338</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the alkaline ceramidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.-" evidence="9"/>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BQ51"/>
    <dbReference type="OrthoDB" id="187171at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016811">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006672">
        <property type="term" value="P:ceramide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071602">
        <property type="term" value="P:phytosphingosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008901">
        <property type="entry name" value="ACER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46187">
        <property type="entry name" value="ALKALINE CERAMIDASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46187:SF3">
        <property type="entry name" value="ALKALINE CERAMIDASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05875">
        <property type="entry name" value="Ceramidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="63"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="91"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="121"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR608901-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR608901-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8030812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="155" mass="18066" checksum="7356AD62AAFD0A20" modified="2023-09-13" version="1">MAPIDGDREGYWGKPTATLDWCEENYAVTPYIAEFWNTVSNVVFFIFPIVTASYFLRDRVERRFLLSCIGFLAVGIGSWCFHMTLRYDMQLLDELPMIWGSCIFLYCIFECHSKRDEHSKGLIAILFLVCAVTTISYIIFVDPYLFQSHRQTTIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BR33</accession>
    <name>A0A9Q1BR33_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_27740</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8031119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BR33"/>
    <dbReference type="OrthoDB" id="5976864at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019073">
        <property type="term" value="P:viral DNA genome packaging"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003389">
        <property type="entry name" value="Adeno_IVa2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02456">
        <property type="entry name" value="Adeno_IVa2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1048">Host nucleus</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8031119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="210" mass="24064" checksum="521E915DB4586A59" modified="2023-09-13" version="1">MNPKLAHPFTCVIAGPTGCGKTQFVKKLLTQDVIEGAPERIVYCYGEYQPAFVEMSAALPQIRFVEGLPTNLDEYLDPSYKTLIILDDIMTSHGNDKRITDLFSKGSHHRNISVIYILQNFFYGAKETRNITLNAHYIVLFKNPRDKSQVSSLGKQMYPGQVKFIQEAFADATKKPYGYLFIDLKPYTRDDFRLRTNIFPGETQYVYLPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BTE8</accession>
    <name>A0A9Q1BTE8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_25813</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BTE8"/>
    <dbReference type="OrthoDB" id="6427388at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331:SF2">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8032319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="238" mass="26769" checksum="A78651CC0917BE5B" modified="2023-09-13" version="1">MVVLSWIKGDPSKYKTFVGNRVGEIHQLVDPSCWNHCPGKDNPADLITRGAYAHRVITSKHWFEGPDWFQESEGTEREETVLASASHTQSVKEMKMKTAVPTSQQDGQVGNPFDVNRWGALAKAVIVVSFVLSGKNVTPGSSIAKLTPFVGKYDLLREKGRLQTSDLSYDEKHLIILLKDYFSLLLIRSYHLKLKNAGVESLISHLRNRYFIVGVRRLTKSGKGTVYHVSDEMRCHAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BTJ1</accession>
    <name>A0A9Q1BTJ1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_23004</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8032913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BTJ1"/>
    <dbReference type="OrthoDB" id="309640at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="CDD" id="cd00448">
        <property type="entry name" value="YjgF_YER057c_UK114_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.40">
        <property type="entry name" value="RutC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035959">
        <property type="entry name" value="RutC-like_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8032913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="84" mass="9272" checksum="7EDC748A505E5830" modified="2023-09-13" version="1">MNEVYSQFFPKNFPARSAFKVAGLALMPYVKRKNPISFGGGQRSFGVTGGPSLKTLLTQYLENAKVEIECIASFRSAIRPLSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BU08</accession>
    <name>A0A9Q1BU08_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_22789</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8032752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BU08"/>
    <dbReference type="OrthoDB" id="6150711at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8032752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="110" mass="12853" checksum="276BE0E185D7E61D" modified="2023-09-13" version="1">MYKARNLLAAIDYQKHKDRTQKVTDGKPVFKRHYFKGSDRWGVIPVKEVKGYDYLPDLMKGVYQKRLEDPFTQRTPLVVGVNDPRKLASTIRPTQPPPTAELVKRHQSRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BU91</accession>
    <name>A0A9Q1BU91_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">P2X purinoreceptor 7 intracellular domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_22791</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8032754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BU91"/>
    <dbReference type="OrthoDB" id="10068685at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046815">
        <property type="entry name" value="P2RX7_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20478">
        <property type="entry name" value="P2RX7_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="P2X purinoreceptor 7 intracellular" evidence="1">
        <location>
            <begin position="69"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF20478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8032754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="139" mass="16185" checksum="E700F8C497341083" modified="2023-09-13" version="1">MINELLPKNSKLYFMRFYMHVFKEQERGGIPLRGRRRRRVMFENMDGQAMRRWLPRLADRQPGLFLDIWELQPRAEGPAHQAQPHWCLCGKCLDMPIAEEELCCAGGQDNCLSDEPEMNALVLDLGVLALRTIHEIFGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BWB3</accession>
    <name>A0A9Q1BWB3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Fucosyltransferase</fullName>
            <ecNumber evidence="11">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_24123</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Golgi apparatus</location>
            <location evidence="11">Golgi stack membrane</location>
            <topology evidence="11">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the glycosyltransferase 10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="11"/>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BWB3"/>
    <dbReference type="OrthoDB" id="427096at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046920">
        <property type="term" value="F:alpha-(1-&gt;3)-fucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11660:FF:000004">
        <property type="entry name" value="Glycoprotein 3-alpha-L-fucosyltransferase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11660">
        <property type="entry name" value="Glycosyl transferase family 10, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055270">
        <property type="entry name" value="Glyco_tran_10_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031481">
        <property type="entry name" value="Glyco_tran_10_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001503">
        <property type="entry name" value="Glyco_trans_10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038577">
        <property type="entry name" value="GT10-like_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11929">
        <property type="entry name" value="ALPHA- 1,3 -FUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11929:SF145">
        <property type="entry name" value="ALPHA-(1,3)-FUCOSYLTRANSFERASE FUT-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17039">
        <property type="entry name" value="Glyco_tran_10_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00852">
        <property type="entry name" value="Glyco_transf_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Fucosyltransferase N-terminal" evidence="13">
        <location>
            <begin position="88"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="Fucosyltransferase C-terminal" evidence="12">
        <location>
            <begin position="186"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF17039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ8033769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="408" mass="48152" checksum="2494D859FD1F2C05" modified="2023-09-13" version="1">MLRHQIFPFRKYSNCFGSKDYIGHRRRTLANTSNNTINSYTLHMQTMQKSYFYDIAIVSMSPVWFTYFTSPREIYCHSTNTSILVRKAIDDEDIITRADILIFSTQFNRVYNWTKFQRLRHSEQLWVYTTEESPFTSLKMYPPVNMNIHFNLSYTYHREADISVPYGQFIPFNLEKQVSDVTSYFHKKTKLIVWVSSHCSFTQMWRRSDFAYDLAKYLPLDMYGSCGNLDCPRKEFSKCVKMFKTYKFYLSIENSCCEGYITEKFWTALTHLETIPVVVGAPKKDYEQIVPKKSFIHADDFGSMKELAEYILKVSKSDEMYNSYFEWKRLGETTMNQIYHILAFNDPGVCKLIEYVKSSSAASLAATSFDPYGPNWFSGCNKCSEQMWLRNYSNLNYDLIMKRLTARI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BWQ6</accession>
    <name>A0A9Q1BWQ6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ficolin-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_24422</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8034014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BWQ6"/>
    <dbReference type="OrthoDB" id="7735550at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050373">
        <property type="entry name" value="Fibrinogen_C-term_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143:SF458">
        <property type="entry name" value="FIBRINOGEN C-TERMINAL DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143">
        <property type="entry name" value="FIBRINOGEN/TENASCIN/ANGIOPOEITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00186">
        <property type="entry name" value="FBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040418252" evidence="1">
        <location>
            <begin position="26"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="2">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="2">
        <location>
            <begin position="128"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8034014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="295" mass="33398" checksum="D6ADBBEF94EA1638" modified="2023-09-13" version="1">MSQFCLCTNAVLILCLCWISGNVLSQDSVIHRLQDGSILFNRNWTEYKTGFGFPSQEFWIGNDKLSFLTNQNTYELRMDVTSVDGLSLFVTYKKFRISDENSEYKLTSVGEYKGEDNITAFVCPSDRVIQCRVNGSQPFFLLWSDYKTGFGTPMHELWLGNDKLHSLTTQKDYELRVDIVNSMGNPYYAKYISFSVSDESDNYRLSLGNYSEGDAGDGLSHSGGKYFTTQDRDNDNWGGGNCATKYADPSCSPRCHNNGAWWYAGNTVVRMYFVAPPPSPVIVGKCIPYQTQEIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BYR6</accession>
    <name>A0A9Q1BYR6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">receptor protein-tyrosine kinase</fullName>
            <ecNumber evidence="2">2.7.10.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_22224</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="2"/>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BYR6"/>
    <dbReference type="OrthoDB" id="9049620at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004714">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013151">
        <property type="entry name" value="Immunoglobulin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050122">
        <property type="entry name" value="RTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416:SF611">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE TRANSMEMBRANE RECEPTOR ROR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00047">
        <property type="entry name" value="ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000615">
        <property type="entry name" value="TyrPK_CSF1-R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040195084" description="receptor protein-tyrosine kinase" evidence="11">
        <location>
            <begin position="25"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="300"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="24"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="279"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="504"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00051243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000615-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ8035108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="650" mass="73683" checksum="A39A2402702B2936" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BYU1</accession>
    <name>A0A9Q1BYU1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_22625</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BYU1"/>
    <dbReference type="OrthoDB" id="6283219at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331:SF1">
        <property type="entry name" value="GAG-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8035402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="466" mass="52480" checksum="F4D65015581845D8" modified="2023-09-13" version="1">MASNGEVPTSVDEGIVSLRQSCNGYVGNLTRRYNELERLMLNADNVAEVETKYAQLEEVYQLYVVKFQALKEKLGSGPDVEKAVSEFEARNQNRMEFAQRVTEYQNGAKSKEILPGKEQTDIPLGLGDIATGGQGSPACSSVCSRKSSTSSAKLREAKLKQELARLKLIQLERKQTLEKQKVEFAQRMEYLDAQDERERADLEVRMWHEETSGYPSLKEEFVKPQWTSADTTGQVPKNLYASMYNVDHGTHNDPINVKIGEGNVKSEIKLPLAAIRNDAPEPFSQILNAGNDEANCSKVPESDMTRLFQSISLSMNIPKPELISFDGDPAAYLGFINSFDTNIALRVKDDRSKLTYHIQFCKGKAKQSIENCVLMDAAAGYKKAREILYDQFGQPHNVTHALIGKILNREPIQPNDGQNLWDLARQMRTCEIILNQLRHVANMNSTDTLLKIQQLLPIYLQSEWAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BYY8</accession>
    <name>A0A9Q1BYY8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_22108</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BYY8"/>
    <dbReference type="OrthoDB" id="8061005at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33198">
        <property type="entry name" value="ANK_REP_REGION DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33198:SF19">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8035031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="145" mass="16857" checksum="67FBDC641510CD41" modified="2023-09-13" version="1">MGKILYKHGKDWKEQFELYLIASHKVTKSDVVKVGILKYSMGPEWIKVANKFTYAAAGDDKKLHKVIQKFDKYFEPKKLVQPYVTRFNKRDQLPSKTLSDYITAIRELASHCEFGALEESQICIRLSNGVRDEKLKEKLWEDGLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BZ92</accession>
    <name>A0A9Q1BZ92_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_22589</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BZ92"/>
    <dbReference type="OrthoDB" id="10651518at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040130603" description="Ig-like domain-containing protein" evidence="2">
        <location>
            <begin position="23"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="255"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="19"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8035378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="351" mass="38020" checksum="6DF565C109C0682C" modified="2023-09-13" version="1">MWLMALSLTLVLAVSFVSPIFAVTTCPNKVLAEKGTDFNITCTIDENTVDLYWYKGKAEEQDLVGKLEMGSVSISQRYRDSYDIGQSGSLRIRNAGLSHESLYTLLTFDSNNTPEEDEVILNITISPYPSCPIISDCTSCTKCYLNNLIGSGSLTCRVEGVRLLIHLSWNATLQTGVGFFERQPVEIVHGSAKSWDISVTLDYNIMNSCGAKAILHGMAEGSSQVLQSTVSTVEINIPCNISGITDNPDTSEKSALLLIVLVVLVLILIVGVLIICCIFCRKHREPSGVVEMDQQDNSDLQILSMGSLSIQCLAFEHVTIDASGNATTISAGAMAFTDHKGVASSNFLALE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BZM3</accession>
    <name>A0A9Q1BZM3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_22587</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BZM3"/>
    <dbReference type="OrthoDB" id="416987at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008042">
        <property type="entry name" value="Retrotrans_Pao"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331:SF1">
        <property type="entry name" value="GAG-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05380">
        <property type="entry name" value="Peptidase_A17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8035376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="732" mass="82186" checksum="92AE93C99A531496" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BZN8</accession>
    <name>A0A9Q1BZN8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_20314</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BZN8"/>
    <dbReference type="OrthoDB" id="8948907at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="532"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="543"/>
            <end position="563"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8036363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="586" mass="65268" checksum="6E01A3270860D488" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C034</accession>
    <name>A0A9Q1C034_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_19462</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8035704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C034"/>
    <dbReference type="OrthoDB" id="6431331at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010463">
        <property type="entry name" value="DUF1057"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47533:SF4">
        <property type="entry name" value="AB HYDROLASE-1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47533">
        <property type="entry name" value="PROTEIN CBG21859"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06342">
        <property type="entry name" value="DUF1057"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8035704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="305" mass="33979" checksum="04ADB12028C38227" modified="2023-09-13" version="1">MKEVTVKALGDQFNVIYAHAGPEGGDVIITIHGSPASHKGFQNMAEPLVAAGYQVIVPNLPGMGWTQLPPSGCYKFSQFEKAQVIQGFAEALKIEKVKMLCGHSLGCHVIYNATRLPSFFARCESVCLISSIGLRPHRLALPWFFKKRFFATMYKMCALPVLGPLLVNIASPIMVKFGLKGRTKLAQVNFFQEGAVFNWEEGTAGLDELCRQKTPALFFFGDKDALVSFDVAQELVARFGITQDNTAWYRGEKKDRDDFKESSLQRAVFVEGGDHSIFTNRVDVITPEQLQFLKSLDSQLQEDEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C197</accession>
    <name>A0A9Q1C197_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_20085</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C197"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8036186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="245" mass="27459" checksum="EC6EEA3AF002F942" modified="2023-09-13" version="1">MATRFYGDLYSSASGAKPKDKWRKIFPAGYKSTAKAVSTFLRYPIALSMKPHYTHTVPFTTDDKLIILFGYKEKFVVYKKLNSTVAILENPVFKQSTPPMMFDGAKVGALLHNIEPKLMYAVAKTDFCEQEGIKFCRNDVLLILAECRTIFLGITEDHGVGFFPSETVEVITKPQLCFFEGMMLSVTAVANFKANEGNQLSVQIGQTYDITKGRDSLFDWFASNHRKVPLHAVNGVSCTDILINL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C1P4</accession>
    <name>A0A9Q1C1P4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_20540</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C1P4"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040309203" evidence="2">
        <location>
            <begin position="23"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8036534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="128" mass="14500" checksum="DA25CF9D06363C50" modified="2023-09-13" version="1">MKILYAGLAIILMFVVFRTSSSQERPDDEGESYYSIIVSAGSRLFTGEHDPTADIGRIGKENIRHRERRKASKEEEGWFVFNQNSVFCFLAMTIFTMGMLVSVYLACCKDENRDKGMIGNFMSGGMPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C3L0</accession>
    <name>A0A9Q1C3L0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_18376</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C3L0"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="190"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="160"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="190"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8037539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="246" mass="28689" checksum="6BE95C57302799D8" modified="2023-09-13" version="1">MESQTMTTQFSQDQHVTGSNYGDKRNWFIYEFVEDTNPKKPIMIAGSVTRQQHKLALCMIGKVFADFTTAEGNNSWVNFRQKFAQYKTEGKVEEGLRTHGFVLERIVPDHTGTPVLQFRVRNRAHLYRLAGKVADNSLAGLLTDYLIPPEEKQTSDVITLRFDQRQFAELEEKIKETERQSMEMYEKYKAMRSVDSEGKDRESPEAEQAYDNSENEAWRQFRNKVMNTGLDVNRQRCVLQNVENFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C429</accession>
    <name>A0A9Q1C429_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_18678</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C429"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8037779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="105" mass="11607" checksum="7472E51F0D431D85" modified="2023-09-13" version="1">MWLAYGIGVEAMYGLREESLAESRCSSDGHLEPYARPVIANEIMKGVNGIPHYTGELFTQGLLIQAVESGKIFDEVEPGSYQVLQVSTKTFQGYFARFSSLPPET</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C4X4</accession>
    <name>A0A9Q1C4X4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_15605</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C4X4"/>
    <dbReference type="OrthoDB" id="10051210at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="CDD" id="cd01644">
        <property type="entry name" value="RT_pepA17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331:SF1">
        <property type="entry name" value="GAG-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8038240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="333" mass="38283" checksum="CB8F638C132A84FE" modified="2023-09-13" version="1">MPENSIKLVNGRYEVGLPWRYPKPYLPNNRVVAEKRLNQLKKRFQRDDNLFQLYRDVIYDYISKGYGRKVTEEPDNTVDQTCKVTSDSDNSSLQLNQSPPQHSQSEADGADEAYVETIKWYLPHQPVLHLRKPGRVRVVFDCASRYKGTCMNDQLLHGPDYTNNLIRVLSRFRRDSVAFIADVEAMFHQVRVPENHSNVLRFLWWPGDNLSMPPVDYQMMVHLFGATSSPSCVSFALKQTAKDNRESFSEEAARTVNESFYVDGCLKSVQTKEQAVALVKELRALLHRGGVRLTKWVSNSREVLETVPEGERAHTVVNLYLDNLPIEHTLGVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C5S5</accession>
    <name>A0A9Q1C5S5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Tetratricopeptide repeat protein 28</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_16520</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="5951504at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024983">
        <property type="entry name" value="CHAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10098">
        <property type="entry name" value="RAPSYN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10098:SF108">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12770">
        <property type="entry name" value="CHAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="34"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="457"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="domain" description="CHAT" evidence="3">
        <location>
            <begin position="730"/>
            <end position="1129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="795"/>
            <end position="816"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8038950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1582" mass="177462" checksum="43C8E220DCADE68A" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C6B3</accession>
    <name>A0A9Q1C6B3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small ribosomal subunit protein uS2m</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">28S ribosomal protein S2, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_16519</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Required for mitoribosome formation and stability, and mitochondrial translation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uS2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C6B3"/>
    <dbReference type="OrthoDB" id="2320368at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01425">
        <property type="entry name" value="RPS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10490:FF:000026">
        <property type="entry name" value="28S ribosomal protein S2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00291_B">
        <property type="entry name" value="Ribosomal_uS2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001865">
        <property type="entry name" value="Ribosomal_uS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005706">
        <property type="entry name" value="Ribosomal_uS2_bac/mit/plastid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018130">
        <property type="entry name" value="Ribosomal_uS2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023591">
        <property type="entry name" value="Ribosomal_uS2_flav_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12534">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S2 PROKARYOTIC AND ORGANELLAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12534:SF0">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00318">
        <property type="entry name" value="Ribosomal_S2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00395">
        <property type="entry name" value="RIBOSOMALS2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52313">
        <property type="entry name" value="Ribosomal protein S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00962">
        <property type="entry name" value="RIBOSOMAL_S2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 9" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00059792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00071390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00083109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8038949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="250" mass="28466" checksum="85197F09CB607328" modified="2023-09-13" version="1">MAQLRSLFRLRTQRLCSSCHFSSSASQVVLPSATTEQVKNPSDPLSDPDYFGLRNLFTLKDLFDARVHLGHKDGLLDPYMKKYLYGIRQRQCILDLEKTFDHLHLALNFAAHIAYRDGIILMINRTPQFSLLVERTAAECGEYAHTREWQEGCFTNAWIQFGPGTRLPDLLIFFHTLNTVFKQHTAIRDSAKMNIPTIGIVDTNCNPNLITYPVPGNDDTPASVELYCKLFKMAILRGKAKRKEDEGKTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C7M8</accession>
    <name>A0A9Q1C7M8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Receptor-type tyrosine-protein phosphatase S</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_17016</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C7M8"/>
    <dbReference type="OrthoDB" id="114660at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26391">
        <property type="entry name" value="INACTIVE TYROSINE-PROTEIN KINASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26391:SF18">
        <property type="entry name" value="PROTEIN KINASE RECEPTOR TIE-1, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0675">Receptor</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040184571" evidence="1">
        <location>
            <begin position="27"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="2">
        <location>
            <begin position="101"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="2">
        <location>
            <begin position="211"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="2">
        <location>
            <begin position="314"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="2">
        <location>
            <begin position="416"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="2">
        <location>
            <begin position="626"/>
            <end position="722"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8039336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="722" mass="80524" checksum="F557503D0B1F7473" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C7W3</accession>
    <name>A0A9Q1C7W3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Sushi, nidogen and EGF-like domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_13913</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8039800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C7W3"/>
    <dbReference type="OrthoDB" id="283575at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120025">
        <property type="term" value="C:plasma membrane bounded cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000247">
        <property type="entry name" value="Delta/notch like EGF repeat containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013032">
        <property type="entry name" value="EGF-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12916">
        <property type="entry name" value="CYTOCHROME C OXIDASE POLYPEPTIDE VIC-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12916:SF4">
        <property type="entry name" value="UNINFLATABLE, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12661">
        <property type="entry name" value="hEGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00010">
        <property type="entry name" value="EGFBLOOD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="9 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2 11" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040469872" evidence="12">
        <location>
            <begin position="28"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="13">
        <location>
            <begin position="29"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="13">
        <location>
            <begin position="69"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="13">
        <location>
            <begin position="110"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="38"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="57"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="95"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="136"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ8039800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="377" mass="40917" checksum="81DE7976406D51D6" modified="2023-09-13" version="1">MQIAINMAESTTNLVCFLVICMHAVWAQNPDVCQPNPCREDGLCIHFPEKNEFVCVCPLGFLGKTCDTEIDECDSSPCQNGGTCEDRNNGFECICASGFRGKTCSEETNLAVVCHHLTCGPQGICLPLGGSFFCICPKGFGGTTCDESLNEDTTTAHHVMTANMTMSPATDPSPASFSFSSTKISRPQTNGISVSTGSLASKNIASFETISPPLTSTIKWLSSLTSRNVPDSQTVETTSTELRLPKTLSSTSFTSTSKEIGISPMITTAQSRSSFSTNPTQTSSTLSFMKEIEILILQGNIGDSTILKWYYDDNDNNRTGCLLFFKLEVKVPQEDGWLFHSSIPATQRFVEIEDIEKRNINLIRIVAVISLAACGHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C9E5</accession>
    <name>A0A9Q1C9E5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">F-box/LRR-repeat protein 12</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_14776</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C9E5"/>
    <dbReference type="OrthoDB" id="3219396at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16134:SF119">
        <property type="entry name" value="AT02038P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16134">
        <property type="entry name" value="F-BOX/TPR REPEAT PROTEIN POF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="75"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8040476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="351" mass="40910" checksum="1E3FA644EA092A85" modified="2023-09-13" version="1">MEAFHLGLHTHNEEVQTESQDTPDCDDADFPFPKHWKKRMLQTYTLTQEEYREIDTLEFPLDLTVVTHIVEELISVNINQLPDDILMQIFSHLDVRDRFRVAGVSRRFNNIIRDRFLWKTVDLRSCSGTLDGRTLHRLLTRYFLSGHLRVMYLPYRFATMGVLKRIGKSCKRLKTLSLSRKQLRPNLNFVVLPESLERIIFDECPFSSRYDYNHLELAGNVRSVVFTNYQKCFDLRRGVLKLKNIRELTIKNCHCFLGEGTLIDVSENNPELETISICGVPDLSDSNVQAICTHFKNVTRLILRQNIGLTEVCLNSLKTMKTLKTIDIGDNPQFDTETLERFLVEHSKINK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CAE2</accession>
    <name>A0A9Q1CAE2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_12106</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CAE2"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8041317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="56" mass="6549" checksum="33ECF12D9C318E1A" modified="2023-09-13" version="1">MWKYFTHRNTLKYIDILPNLVKGTIILTTDRLNCAQWKLQKITKIGCGEPYIPALI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CCC4</accession>
    <name>A0A9Q1CCC4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_13062</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8042080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CCC4"/>
    <dbReference type="OrthoDB" id="8064693at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8042080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="132" mass="15113" checksum="373A39820CF901C7" modified="2023-09-13" version="1">MSQKLAVDDNLKHPPSKSEDTVPKSGTNDTTRTNLIEHDVDVGDATPIKQHPYRVNPRKRQHLRDEVRYMLDHDIIEPSNSEWSSPCILVPKPDVIRSWNQFLRYSTLQVYYCTIVNVHALPALGPDQANIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CCE4</accession>
    <name>A0A9Q1CCE4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_13099</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8042110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CCE4"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8042110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="169" mass="19083" checksum="A790AF1DD804612B" modified="2023-09-13" version="1">MRLVLLLFGYHSESVAKGGRRVREGEDGEDLNMMAFREPEIVQAVATQDDLIKRQSKIGSFFRSQLRNSFRSSFRIWGSADALNSDRSKSTSHTPPKTPPSSPRLRSKSAQHELRSDNYLMPPPNPFHGRKSKKKAGRRSRSPDPAGMKRSGSDSDLLDDSPHHHIYII</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CDC2</accession>
    <name>A0A9Q1CDC2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Dual specificity protein phosphatase 22</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_10209</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4 9">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 9">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CDC2"/>
    <dbReference type="OrthoDB" id="9979246at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45948:SF2">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45948">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE DDB_G0269404-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="6">
        <location>
            <begin position="37"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8043217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="121" mass="13986" checksum="86A559669687DC05" modified="2023-09-13" version="1">MRFYCTIYAVTPFQTINTICSFSLATTKHQQYIMGQGMSEILPGIFLGSFRDCKDKDQMEKNHITHILAIHDNAKPIIEGKTYLCITASDKHNENILQYFEECILFIHQCRQSGGKVLIHW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CGA1</accession>
    <name>A0A9Q1CGA1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Gamma-aminobutyric acid type B receptor subunit 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_07131</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CGA1"/>
    <dbReference type="OrthoDB" id="17569at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038039">
        <property type="term" value="C:G protein-coupled receptor heterodimeric complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004965">
        <property type="term" value="F:G protein-coupled GABA receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007214">
        <property type="term" value="P:gamma-aminobutyric acid signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002455">
        <property type="entry name" value="GPCR3_GABA-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10519">
        <property type="entry name" value="GABA-B RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10519:SF74">
        <property type="entry name" value="GAMMA-AMINOBUTYRIC ACID TYPE B RECEPTOR SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Receptor ligand binding region" evidence="9">
        <location>
            <begin position="40"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8044395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="147" mass="16739" checksum="8B133999497327DB" modified="2023-09-13" version="1">MMSMSGEWDGSGCLVAAELALEQINNRTDILPDYELIMPWNDTQVLYSATSNTLTDRVKYPNVYRTVPIATNTFNSPYIWLLRTYGWKKVGFLSDSDASFTAVSLEFYLSMFFPSSRHMVYLFLCSSRISSVSEMQVKIVLKVNGEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CH00</accession>
    <name>A0A9Q1CH00_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_08102</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CH00"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8045157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="68" mass="7851" checksum="1480785FC1B3493C" modified="2023-09-13" version="1">MESSREFNFNAAFLELSRSENCLRSSPSLVVIRSWNQFLRYSTLQVYYRTIVDVHALPALGPDQANIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CIC1</accession>
    <name>A0A9Q1CIC1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Zinc transporter 6-A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_07937</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Has probably no intrinsic transporter activity but together with SLC30A5 forms a functional zinc ion:proton antiporter heterodimer, mediating zinc entry into the lumen of organelles along the secretory pathway. As part of that zinc ion:proton antiporter, contributes to zinc ion homeostasis within the early secretory pathway and regulates the activation and folding of enzymes like alkaline phosphatases and enzymes involved in phosphatidylinositol glycan anchor biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Heterodimer with SLC30A5; form a functional zinc ion transmembrane transporter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CIC1"/>
    <dbReference type="OrthoDB" id="5382797at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006829">
        <property type="term" value="P:zinc ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="entry name" value="Cation efflux protein transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058533">
        <property type="entry name" value="Cation_efflux_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052005">
        <property type="entry name" value="CDF_SLC30A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46531">
        <property type="entry name" value="ZINC TRANSPORTER 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46531:SF1">
        <property type="entry name" value="ZINC TRANSPORTER 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="Cation efflux protein transmembrane domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="42"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="80"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="141"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="173"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein transmembrane" evidence="13">
        <location>
            <begin position="1"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="291"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="337"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="409"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="304"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="344"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="409"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00045455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ8045019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="451" mass="49521" checksum="68232A57E5927735" modified="2023-09-13" version="1">MALTAYSTLLLFDLLSLGTSLVSIWVSKQKTSSAYSFGYERFEVLAVFTSTMLASFSAIFIIKECTERIFSQPEIHTGRLMLGAALGFLCHMVVTYGNRNHALSHVSTSSKSSWLQEHFADICKSFCTVVPGLNHLLLPRINPFALVGFGGATALSVADFLIDVNNYHSADTWASYAIAFMLFGTMFPLSVYSGTILLQTTPAHLLGQLDKCLREASTLDGVLEFRNEHFWTLSFGRLAGSLHVRVRRDADEQLVLAHVTNKLSHLVSPLTIQIIKDDWTRVSSISSFSSGHLDNKLTPPTPLPSQYSSAALTKTSHHIPASSVQHLLQQKRLGPVDSMRHPRASSTPFGTPSKDSGISLNMSEPSPILPYIGGGASQSRRAFGETKSKGILRNAPYVNIPLESNYNQAPSWNKHSQEIHPSNSVTVDLSQDRNTPLLSEKQYRGGSSAHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CJT4</accession>
    <name>A0A9Q1CJT4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_05412</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CJT4"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8046657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="65" mass="7468" checksum="2199FB5F91A5F13C" modified="2023-09-13" version="1">MFTVLDAGQASRQVKLTESSRHYVKFNTPYGRYQFCISPFGLCSAPARYFKNGSIKFLRVSQVLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CL71</accession>
    <name>A0A9Q1CL71_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">N-terminal amino-acid N(alpha)-acetyltransferase NatA</fullName>
            <ecNumber evidence="4">2.3.1.255</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_06395</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>N-terminal L-alanyl-[protein] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-alanyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50500"/>
            <dbReference type="Rhea" id="RHEA-COMP:12701"/>
            <dbReference type="Rhea" id="RHEA-COMP:12702"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:64718"/>
            <dbReference type="ChEBI" id="CHEBI:83683"/>
            <dbReference type="EC" id="2.3.1.255"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>N-terminal L-cysteinyl-[protein] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-cysteinyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50512"/>
            <dbReference type="Rhea" id="RHEA-COMP:12707"/>
            <dbReference type="Rhea" id="RHEA-COMP:12708"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:65250"/>
            <dbReference type="ChEBI" id="CHEBI:133372"/>
            <dbReference type="EC" id="2.3.1.255"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>N-terminal L-seryl-[protein] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-seryl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50504"/>
            <dbReference type="Rhea" id="RHEA-COMP:12703"/>
            <dbReference type="Rhea" id="RHEA-COMP:12704"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:64738"/>
            <dbReference type="ChEBI" id="CHEBI:83690"/>
            <dbReference type="EC" id="2.3.1.255"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N-terminal L-threonyl-[protein] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-threonyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50516"/>
            <dbReference type="Rhea" id="RHEA-COMP:12709"/>
            <dbReference type="Rhea" id="RHEA-COMP:12710"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:64739"/>
            <dbReference type="ChEBI" id="CHEBI:133375"/>
            <dbReference type="EC" id="2.3.1.255"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>N-terminal L-valyl-[protein] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-valyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50508"/>
            <dbReference type="Rhea" id="RHEA-COMP:12705"/>
            <dbReference type="Rhea" id="RHEA-COMP:12706"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:64741"/>
            <dbReference type="ChEBI" id="CHEBI:133371"/>
            <dbReference type="EC" id="2.3.1.255"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>N-terminal glycyl-[protein] + acetyl-CoA = N-terminal N(alpha)-acetylglycyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50496"/>
            <dbReference type="Rhea" id="RHEA-COMP:12666"/>
            <dbReference type="Rhea" id="RHEA-COMP:12700"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:64723"/>
            <dbReference type="ChEBI" id="CHEBI:133369"/>
            <dbReference type="EC" id="2.3.1.255"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the acetyltransferase family. ARD1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.255" evidence="4"/>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CL71"/>
    <dbReference type="OrthoDB" id="25586at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031415">
        <property type="term" value="C:NatA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990189">
        <property type="term" value="F:protein N-terminal-serine acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990190">
        <property type="term" value="F:protein-N-terminal-glutamate acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000101">
        <property type="entry name" value="N-alpha-acetyltransferase 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045047">
        <property type="entry name" value="Ard1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23091">
        <property type="entry name" value="N-TERMINAL ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23091:SF4">
        <property type="entry name" value="N-TERMINAL AMINO-ACID N(ALPHA)-ACETYLTRANSFERASE NATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="12">
        <location>
            <begin position="1"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="182"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="192"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="260"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00026110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8047407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="269" mass="30963" checksum="4D5D365E8D42ACB0" modified="2023-09-13" version="1">MNIRRASPQDLMNMQHCNLLCLPENYQMKYYFYHGLSWPQLSYVAEDENQKVVGYVLAKMEEDPDEKPHGHITSLAVKRSHRRLGLAQKLMDQASRAMVECFNAQYVSLHVRKSNRAALHLYQNTLKFEVSEIEPKYYADGEDAYAMRRDLTNFLNKSSKDKDKSKSDIKDEITEQINRASQGLEKLAISDNKMETEKNAEEQKKKGENQEKNVEEKKKETSEKPGDDGKKRDNTEKGRGGGEGKTGKESRKGRRKNREGHKTEEKGAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CLP7</accession>
    <name>A0A9Q1CLP7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_06564</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CLP7"/>
    <dbReference type="OrthoDB" id="10029313at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21301">
        <property type="entry name" value="REVERSE TRANSCRIPTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21301:SF10">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8047538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="233" mass="26830" checksum="1B19F556FD24F904" modified="2023-09-13" version="1">MPPKSSIPEVEVFLKNLRADILNPENTRAPKDNLSREERKAIHKHKEDDRVIRIQDKGSRFVIMDKNDYQTKMEQQLTNPLHYNIMTEDPSNKFHQEVTHWAYKWLRRQQISEEIFNWITEVIPKPGVAYGNVKTHKDGNPVRLITSCCGTAIERLSAFTEFYLKPLAQNLPSFLKDTSDLLNKIKAVNNSGPLPANTILVSWDVVAMFPNIDNKLGISAVSKYLNTRENQNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CLS4</accession>
    <name>A0A9Q1CLS4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">oleoyl-[acyl-carrier-protein] hydrolase</fullName>
            <ecNumber evidence="1">3.1.2.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_06787</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.14" evidence="1"/>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="329835at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004312">
        <property type="term" value="F:fatty acid synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016297">
        <property type="term" value="F:fatty acyl-[ACP] hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05274">
        <property type="entry name" value="KR_FAS_SDR_x"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00833">
        <property type="entry name" value="PKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000019">
        <property type="entry name" value="Polyketide synthase type I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.366.10">
        <property type="entry name" value="Malonyl-Coenzyme A Acyl Carrier Protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.110">
        <property type="entry name" value="Polyketide synthase dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001227">
        <property type="entry name" value="Ac_transferase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014043">
        <property type="entry name" value="Acyl_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014031">
        <property type="entry name" value="Ketoacyl_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014030">
        <property type="entry name" value="Ketoacyl_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057326">
        <property type="entry name" value="KR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016036">
        <property type="entry name" value="Malonyl_transacylase_ACP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032821">
        <property type="entry name" value="PKS_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020841">
        <property type="entry name" value="PKS_Beta-ketoAc_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042104">
        <property type="entry name" value="PKS_dehydratase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020807">
        <property type="entry name" value="PKS_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049551">
        <property type="entry name" value="PKS_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049552">
        <property type="entry name" value="PKS_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013968">
        <property type="entry name" value="PKS_KR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049900">
        <property type="entry name" value="PKS_mFAS_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050091">
        <property type="entry name" value="PKS_NRPS_Biosynth_Enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001031">
        <property type="entry name" value="Thioesterase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43775">
        <property type="entry name" value="FATTY ACID SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43775:SF37">
        <property type="entry name" value="SI:DKEY-61P9.11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00698">
        <property type="entry name" value="Acyl_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16197">
        <property type="entry name" value="KAsynt_C_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00109">
        <property type="entry name" value="ketoacyl-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02801">
        <property type="entry name" value="Ketoacyl-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08659">
        <property type="entry name" value="KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21089">
        <property type="entry name" value="PKS_DH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14765">
        <property type="entry name" value="PS-DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00975">
        <property type="entry name" value="Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00827">
        <property type="entry name" value="PKS_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00826">
        <property type="entry name" value="PKS_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00822">
        <property type="entry name" value="PKS_KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00825">
        <property type="entry name" value="PKS_KS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55048">
        <property type="entry name" value="Probable ACP-binding domain of malonyl-CoA ACP transacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52004">
        <property type="entry name" value="KS3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52019">
        <property type="entry name" value="PKS_MFAS_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ketosynthase family 3 (KS3)" evidence="6">
        <location>
            <begin position="30"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="PKS/mFAS DH" evidence="7">
        <location>
            <begin position="971"/>
            <end position="1267"/>
        </location>
    </feature>
    <feature type="region of interest" description="N-terminal hotdog fold" evidence="5">
        <location>
            <begin position="971"/>
            <end position="1101"/>
        </location>
    </feature>
    <feature type="region of interest" description="C-terminal hotdog fold" evidence="5">
        <location>
            <begin position="1118"/>
            <end position="1267"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for dehydratase activity" evidence="5">
        <location>
            <position position="1008"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor; for dehydratase activity" evidence="5">
        <location>
            <position position="1181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS52004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS52019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8047722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="2496" mass="278180" checksum="AC25A437828F1FBC" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CPV5</accession>
    <name>A0A9Q1CPV5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_00582</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CPV5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8048319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="107" mass="12224" checksum="3626C52CFDD0A290" modified="2023-09-13" version="1">MESSREFNFNAAFLELSRRENCLRGLTTLTLLRSYSQPLTLHTCDKLPYAVSALCTEIVIIHHSSPSLVIIRSWNQFLRYSTLQVYYRTIVNVHALPALGPDQANIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CRQ8</accession>
    <name>A0A9Q1CRQ8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sulfotransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_04210</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CRQ8"/>
    <dbReference type="OrthoDB" id="10019582at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053259">
        <property type="entry name" value="Golvesin-related_Golgi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005331">
        <property type="entry name" value="Sulfotransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32301">
        <property type="entry name" value="COUNTIN RECEPTOR CNR3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32301:SF6">
        <property type="entry name" value="GOLVESIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03567">
        <property type="entry name" value="Sulfotransfer_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8050852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="759" mass="89335" checksum="5A052201534EF931" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CRW3</accession>
    <name>A0A9Q1CRW3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Cytochrome P450 18a1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_02847</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CRW3"/>
    <dbReference type="OrthoDB" id="2789670at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016712">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008395">
        <property type="term" value="F:steroid hydroxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006805">
        <property type="term" value="P:xenobiotic metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002397">
        <property type="entry name" value="Cyt_P450_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050182">
        <property type="entry name" value="Cytochrome_P450_fam2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300:SF403">
        <property type="entry name" value="CYTOCHROME P450 306A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300">
        <property type="entry name" value="CYTOCHROME P450 508A4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00359">
        <property type="entry name" value="BP450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0349">Heme</keyword>
    <keyword evidence="3 4" id="KW-0408">Iron</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8049900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="103" mass="11719" checksum="CFA4ADE7BB012CFA" modified="2023-09-13" version="1">MDPTAFPNPEEFDPERFFDTNGNFRKPKELVPFGMGMRSCAGAHLAQMEFFLITCSILQKMTLKNPPGQDMPTVFTSENGFSRSPRPFKVLVRLRKTELSKGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CT14</accession>
    <name>A0A9Q1CT14_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CRC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_03446</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the lin-54 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CT14"/>
    <dbReference type="OrthoDB" id="5984512at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005172">
        <property type="entry name" value="CRC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033467">
        <property type="entry name" value="Tesmin/TSO1-like_CXC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46704:SF9">
        <property type="entry name" value="BHLH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46704">
        <property type="entry name" value="CXC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03638">
        <property type="entry name" value="TCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01114">
        <property type="entry name" value="CXC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51634">
        <property type="entry name" value="CRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRC" evidence="4">
        <location>
            <begin position="109"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8050300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="163" mass="18405" checksum="635B9D91028ACD8C" modified="2023-09-13" version="1">MCSALIGFHVFTGCDSVSSFMERPVCTDVNQARLHLFKLDVRSETGMPPNQDALQKHILRANYQAAIHKACMEQYPEIPPPVNHGWKLVDNNLVIDWMDRPPAPDSILEFVQCGCKKSKCQQRNCSCVQNGLPCTDLCHCSNCENKFVSDEMEDEVDTDEEVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1GXM4</accession>
    <name>A0A9Q1GXM4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SRCR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_32790</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1GXM4"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00530">
        <property type="entry name" value="SRCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="69"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="4">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="8"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8027599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="160" mass="18615" checksum="893FC99073185460" modified="2023-09-13" version="1">MVVDQVNCSGEETTLLQCKHKNDSNCKYDEDVFVVCIAEEDFDDEKKEKMEKERPVDPMEQDQKGSSSVLIIFVVLFGILVCILAVTSVYRHWTIRCIQKRNPITSKVERPLPTPQQELKYDIKEQEEGQYQIIEENLEVTPYNREIHNSEKETSTKTSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H0S3</accession>
    <name>A0A9Q1H0S3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF4190 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_30733</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H0S3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8028485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="143" mass="15160" checksum="ED39EC28D0381FEE" modified="2023-09-13" version="1">MEHLVKNEHLPATSDPPIVAASPTSVNMQPPPYEPVLPQQTVLVRSTYNEAPPGVTAKAIVSIVLSVIAFFCSYVTAFCAIPSIVFGVMALSSDYSDNQRKSYANTALGLMLGGVVTAVIIVVLVVVLLINAGSESSSYHGYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H1J0</accession>
    <name>A0A9Q1H1J0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">G-protein coupled receptor Mth2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_24923</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H1J0"/>
    <dbReference type="OrthoDB" id="6134459at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15039">
        <property type="entry name" value="7tmB3_Methuselah-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053231">
        <property type="entry name" value="GPCR_LN-TM7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001212">
        <property type="entry name" value="Somatomedin_B_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45902">
        <property type="entry name" value="LATROPHILIN RECEPTOR-LIKE PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45902:SF1">
        <property type="entry name" value="LATROPHILIN RECEPTOR-LIKE PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00249">
        <property type="entry name" value="GPCRSECRETIN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50958">
        <property type="entry name" value="SMB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040441762" evidence="7">
        <location>
            <begin position="25"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="569"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="599"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="627"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="677"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="722"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="775"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="802"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="domain" description="SMB" evidence="9">
        <location>
            <begin position="68"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="8">
        <location>
            <begin position="567"/>
            <end position="826"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
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    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8031662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="853" mass="97671" checksum="57EE72609517E360" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H298</accession>
    <name>A0A9Q1H298_HOLLE</name>
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        <recommendedName>
            <fullName evidence="5">ALMS motif domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_27371</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
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        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6359887at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046599">
        <property type="term" value="P:regulation of centriole replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="ALMS_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21553:SF26">
        <property type="entry name" value="ALMS MOTIF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21553">
        <property type="entry name" value="ALMS1-RELATED"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF15309">
        <property type="entry name" value="ALMS_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ALMS motif" evidence="5">
        <location>
            <begin position="2182"/>
            <end position="2326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
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        </location>
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        </location>
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        </location>
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            <begin position="200"/>
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        </location>
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        </location>
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        </location>
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        <location>
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            <end position="2319"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF15309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8030839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="2333" mass="262650" checksum="F3E2174C33E63243" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H2L6</accession>
    <name>A0A9Q1H2L6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_29397</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H2L6"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="21"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8029881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="98" mass="11481" checksum="503674AC2D6999A5" modified="2023-09-13" version="1">MEQKLDIMEQINKLQSLPSQLEAMQQEIKSLRGAVSDMAKSIKFMEDKVDKVKKTMKAKADRDEVKSLKDSVEEMEMKMKQKLEDLGNRSRRQNLVFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H2U8</accession>
    <name>A0A9Q1H2U8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_26917</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Echinodermata</taxon>
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            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
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            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H2U8"/>
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        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8030480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="184" mass="21016" checksum="642051A8CEEC2F61" modified="2023-09-13" version="1">MIAYVNHSKEAIIIQHQDTSQDLWVLERQNVYDFTRLCTSDVYSYPLNADPTFNFANFHLTPFIYCHILLENKRSGVDLILLGPTAIHHSRLQPLYRKTVSAVTGNCEKLKERCKGFMTDAEQAIEDALEVMMPLRDFNHLGQNCKSKLISTPLQQKLFLDAVCGVQEERNGITDAKDGKDLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H3Q2</accession>
    <name>A0A9Q1H3Q2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_25056</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H3Q2"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8031753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="93" mass="10563" checksum="A57D729B8862CC40" modified="2023-09-13" version="1">MASLPADRVTPNKPPFAHTGVDCFGSFYGKRGRWQERGMAVCSPVSPQEQYTLKCSTLWTHIFSRRATQEKIRSNNGTNFVRGSKELQESIRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H3U1</accession>
    <name>A0A9Q1H3U1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_24691</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H3U1"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="234"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="316"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="374"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="331"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="408"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8031485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="437" mass="48185" checksum="41F3AC8D5854B759" modified="2023-09-13" version="1">MSTSCGSMRWTNDSRRGTSPFIESSINTFVNSNGDSYELRIFNMSAYDVGSYSCWCKENNELMYFICSDSLKLICQAKVVVNNTTKLHNSSRTDSFSEITINANVRDRLKVKCPDGAFPTTDCKNVLEIESGQFTYNFKVSSENDLCRITCQVQRETRVVCSITFTINVSKNETSTHNSAVSNAMFNVSPPVNETAFTNFSSENINLPPTYATVQVDEKKNNNLYPATNKPASFSAMTVVGILIFIVVVLMFVAGFHIFSSRFEIGSWSTKTANPPHSTDANIPVGPTYATVNIATNGFVKGDDHSIEPEFLNSDGLYSEVDGAGKEDSSTHQPNPQTAETSITNEYYSEVNTSDAKLKTIENGQETDSSLYTEINEGDNQPKQKECTSSPQSSGDDARVSDVPIYHVLEEPKRSEKDTEGNVSTSQVLHAVEDTDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H4A4</accession>
    <name>A0A9Q1H4A4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Sepiapterin reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_25345</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H4A4"/>
    <dbReference type="OrthoDB" id="153074at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004757">
        <property type="term" value="F:sepiapterin reductase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006729">
        <property type="term" value="P:tetrahydrobiopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051721">
        <property type="entry name" value="Biopterin_syn/organic_redct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44085">
        <property type="entry name" value="SEPIAPTERIN REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44085:SF2">
        <property type="entry name" value="SEPIAPTERIN REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0521">NADP</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8031965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="244" mass="27726" checksum="9C4197574DECC078" modified="2023-09-13" version="1">MARKVDPTSFMYLIDIQQDGIEDTARAFKQDVSDERLKVRCIVADLSDEKSVKELNSSIFKDDLDPSIFSHIILVHNAGATGFTEKHARELNDIESIQKYFLLNLNSAILLTSGFLQHFHNKSDIRKTVAQMTSYAAIYPQQSLHLYGAVKAGRDMFFRVMALEEPDVKFLAFNPGNVDTNMYREGYGSHYAMFRPMLEKVEKQPYFLTPQQAAGALVKTIEEDKYESAVTVNSYDVLGIKVDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H4H3</accession>
    <name>A0A9Q1H4H3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Nucleolar protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_23131</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H4H3"/>
    <dbReference type="OrthoDB" id="10047222at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039788">
        <property type="entry name" value="NOL4/NOL4L"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12449">
        <property type="entry name" value="DEATH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12449:SF22">
        <property type="entry name" value="NUCLEOLAR PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8033014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="283" mass="32496" checksum="EC09A55DE33B505E" modified="2023-09-13" version="1">MGKLICSDEVNISRYRDWALRNYGDSGKTKTVTKRKYERIVKVLRGKDRFSGSASSENGKFRFWVRGKGFKLGKPLGYPVVGLGDEVLLVPVKTTNSDGTIIDKGYKQVAVVEDFYDIIYNVHVQTEGKEGKHAGQKRTYRAVAEKFAFLPREAVTRFLMSCKDCKTRMQLTFESSDKKKESQLSAETKDHELENEVPDIRYSLPITTTYMSHLRNLQYLSETEVRLWHHFKISFTLQMLGPPRFLESVDTTRLAVQSNTEQASFISTKYTNMEECPLTQNTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H4L4</accession>
    <name>A0A9Q1H4L4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Tick transposon</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_25539</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H4L4"/>
    <dbReference type="OrthoDB" id="10025340at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00303">
        <property type="entry name" value="retropepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000032">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 297-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon gypsy-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF1">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="4">
        <location>
            <begin position="304"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase catalytic" evidence="5">
        <location>
            <begin position="701"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="259"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="342"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="38"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="259"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="274"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8032116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="853" mass="95731" checksum="D3A6FF04A5D6B4AA" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H4S1</accession>
    <name>A0A9Q1H4S1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ubiquitin-fold modifier-conjugating enzyme 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_22652</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ubiquitin-conjugating enzyme family. UFC1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H4S1"/>
    <dbReference type="OrthoDB" id="10256182at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061657">
        <property type="term" value="F:UFM1 conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990592">
        <property type="term" value="P:protein K69-linked ufmylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11686">
        <property type="entry name" value="UBCc_UFC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000042">
        <property type="entry name" value="Ubiquitin-fold modifier-conjugating enzyme 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014806">
        <property type="entry name" value="Ufc1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12921">
        <property type="entry name" value="UBIQUITIN-FOLD MODIFIER-CONJUGATING ENZYME 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12921:SF0">
        <property type="entry name" value="UBIQUITIN-FOLD MODIFIER-CONJUGATING ENZYME 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08694">
        <property type="entry name" value="UFC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF008716">
        <property type="entry name" value="DUF1782"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="4">
        <location>
            <position position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR008716-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8035427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="201" mass="23183" checksum="AEF3D35CD8DCF6E8" modified="2023-09-13" version="1">MYCIVKEKRIIYVNKEIYAKLWKYQPSLTSIKMVDAATKKALSGIPLLKTNAGPRDKDLWPTRLKEEYMSLIKYVENNKAADNDWFRLESSKDGTRWFGKCWHMQDFLKYEFDIEFDIPVTYPVTAPEIAIPELDGKTAKMYRGGKICLTDHFKPLWAKNVPKFGIGHAIALGLGPWLAVEIPDLISKGLVKHKDEKEEET</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H6D2</accession>
    <name>A0A9Q1H6D2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_21279</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H6D2"/>
    <dbReference type="OrthoDB" id="5984724at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331:SF5">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8034438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="73" mass="8323" checksum="8191BC18CD22CFB5" modified="2023-09-13" version="1">MADLPTERVEDSTPPCSYVGIDVFGPFYVRLGRSEVKRYGCLFTCLSIRAIHVEKLDSLDTDTFINAFRRFVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H6W5</accession>
    <name>A0A9Q1H6W5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_21558</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H6W5"/>
    <dbReference type="OrthoDB" id="10062880at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31025">
        <property type="entry name" value="SI:CH211-196P9.1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31025:SF9">
        <property type="entry name" value="SI:DKEY-286J15.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8034636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="518" mass="59123" checksum="41987F8E8B9440B2" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H8Y0</accession>
    <name>A0A9Q1H8Y0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_17688</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8036990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H8Y0"/>
    <dbReference type="OrthoDB" id="6625098at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8036990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="91" mass="9832" checksum="B8E930E2D02B3331" modified="2023-09-13" version="1">MKPFYCSLLFDGRTDKSLSEKEIIIIKYLDEGVPTIKLLSITEPESGKAEAVFEAIKAKAEDMDLSLGRSCIAMAAHGASVNFGSQSGMMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H9I2</accession>
    <name>A0A9Q1H9I2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">alpha-1,2-Mannosidase</fullName>
            <ecNumber evidence="7">3.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_15279</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the glycosyl hydrolase 47 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.-" evidence="7"/>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H9I2"/>
    <dbReference type="OrthoDB" id="8118055at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044322">
        <property type="term" value="C:endoplasmic reticulum quality control compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004571">
        <property type="term" value="F:mannosyl-oligosaccharide 1,2-alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904380">
        <property type="term" value="P:endoplasmic reticulum mannose trimming"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044674">
        <property type="entry name" value="EDEM1/2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001382">
        <property type="entry name" value="Glyco_hydro_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036026">
        <property type="entry name" value="Seven-hairpin_glycosidases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45679">
        <property type="entry name" value="ER DEGRADATION-ENHANCING ALPHA-MANNOSIDASE-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45679:SF2">
        <property type="entry name" value="ER DEGRADATION-ENHANCING ALPHA-MANNOSIDASE-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01532">
        <property type="entry name" value="Glyco_hydro_47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00747">
        <property type="entry name" value="GLYHDRLASE47"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48225">
        <property type="entry name" value="Seven-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PA" evidence="9">
        <location>
            <begin position="614"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="452"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="485"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="495"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="88"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="231"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="325"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="343"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="429"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8040857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="710" mass="79351" checksum="5C246958EDB63C78" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H9I8</accession>
    <name>A0A9Q1H9I8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Caspase recruitment domain-containing protein 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_18971</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8038009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H9I8"/>
    <dbReference type="OrthoDB" id="8868836at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="220"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="67"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="278"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="222"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8038009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="413" mass="47916" checksum="5E4E497FAF8CB1E5" modified="2023-09-13" version="1">MTGEMLDILKTKGPRGYSIFINVLGIRNPGIYTEMTGKEAPEQPIPLSCNCNCHHDGSGTLPYLEQLTEALKKKEQLEGEVRALKNHNLKLSSQSKGVAEKLDKLWRRYKVEKEKNRDRDRFEEERNEMMEQLTGMTGKALNYKEQLEVATNNLHQSRLEILQLKRELEKANRGLKHQRQRSMQIERSYSHSRTLIKNTEELQEKVARLELELCKADKGESNRASYIQPSSDDNNESTGQVSASNKDSRLIQLQTDLEIMRNDRDEALGKAEDAVSQMYKVTKELHEVEEKLEKLSREKDDLLLSLESQKDEADMQKRFVLLAEDQLGRAVEERNQALEEKNLQTTKCARLMLQRDSLIEQQRALRADMTNLKEKQLVAEAKMVKYKAKLRVSVFRSLFSRDFYVKLRQQISV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H9L5</accession>
    <name>A0A9Q1H9L5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Dehydrogenase/reductase SDR family member 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_18131</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H9L5"/>
    <dbReference type="OrthoDB" id="1669814at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004090">
        <property type="term" value="F:carbonyl reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005559">
        <property type="entry name" value="PRK07231.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43943">
        <property type="entry name" value="DEHYDROGENASE/REDUCTASE (SDR FAMILY) MEMBER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43943:SF2">
        <property type="entry name" value="DEHYDROGENASE_REDUCTASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8037336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="276" mass="30090" checksum="D14E3B97C078CBCE" modified="2023-09-13" version="1">MSFYYSDEYLQKRFTGKVAICTASTNGIGYATAERLAREGAKVVVSSRRQKNVDDAVKRLRDQNLEVSGIVCHQAKKEDRQRLIDFTLTTYGGIDLLFVSAGVNPHKGHMLGISEKEWDKLFDVNVKSTFLMIRECIPHMIQRGGGAIVSNATVSALHPNLRMASEYLGPYATSKCAVLGLTKVLASPLATHKIRINCIAPGAFKTNFLEGTYKQYFPDQTAVPSFINRAALMDRTGDPSECGSVVAFLLSDDASFITGEILTVSGGTVLSPAARL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HB04</accession>
    <name>A0A9Q1HB04_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_16504</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HB04"/>
    <dbReference type="OrthoDB" id="6380096at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF1">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="4">
        <location>
            <begin position="291"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="257"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="49"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="265"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8038938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="317" mass="36360" checksum="64AD01F4C53A470C" modified="2023-09-13" version="1">MSQVGDLEIEKLMAVGKTFGLKDVALKEFVETERARAKAERDVERKFRAEEREQEKEKLEIELKLAALKKENAGLVDQRPHEMSNKAKPPKLPCFTDKDDLDAYLDRFERYAKVQNWAEGEWAINLASLLTGKALYTYSTLPVSEANEYQSLKLALLKRFNLTPTGYKTKFRTCKPDLNETASQYVTRLANYFDRWIDTSQVQHQFKDLREHLILDQFLSSAPKEVAIFVKERKLKTLGAASQLADEYIDVHRGWRRNPNVAPKETGHKGSSAEKDVSISKTKSSNARPVCYYCGKPGHFWKQCRDAPKSFVPTMAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HBJ2</accession>
    <name>A0A9Q1HBJ2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_14220</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HBJ2"/>
    <dbReference type="OrthoDB" id="10064694at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="318"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8040034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="343" mass="38864" checksum="C044EEB3CCA690EA" modified="2023-09-13" version="1">MKPLSNAWNNSKRYSGLVDAKKNDVRIDNPDAHYYCARTRYRLEFAMEFEESIILSADCMNKINVGGLAVSRYHQVNKIFPTDDAPNLPDHDFPMGSNYKLTPSGYLQLNRDKSKPKVTKDKLGRDIFPAPRSGPAFVFNRAASFRSVSSNLTAEERRTKEAIVFDSAMGRLSEYWHGAQFDSHPVTVYSEKCTEVDRPFNDYIDVHGALKTATRKKLKGTNDALQAEIDFLARHSDRRIGELTMLKCEDTNCDHCSGSVPSSALLKVRLHGGMPTPVTDTSICKEGDSHYFTYSQAIRRQFQPPDEEMVIFQLKELGKCDVKGCRYVFASKKDLAQHSKLFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HCC9</accession>
    <name>A0A9Q1HCC9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_15281</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HCC9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="209"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="250"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="292"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="323"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8040859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="378" mass="43565" checksum="E1248CC40579B760" modified="2023-09-13" version="1">MDRDKLVCERRNGLVKCRGQLVRYDTSSGLENQAFCTKCKAEWMVSKDEDGNVTFDKRTSFRKTLCTKEKPVTGSQDLQRGDHVTWEEVEGRHHAIVENVADTSQVEVFASNLTADGGKLEIVQSRIDLTNSSLSLLEYDNEWHIYNPPEQVLLRARSLSIHSGTFNSDEQFATFCKTGHFQKQKFTHTMRELYEDVRIVYPTIFSLELLTNVLVLLLNILLSIFIETLAECGKCRKLAKLTLSPCSRRYWFLSEILLILFEGLVCLFYVIYQLYYKPRVKDSDNNGKYRTHTIYLAIPYVCEYVCAVLLASLSLFCNLRGTFCVLWCKVFIGTIFGVLGKLLGYVMGRVIFKRVTYKSIRTEITESYRKVDQNPSQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HCE2</accession>
    <name>A0A9Q1HCE2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tesmin/TSO1-like CXC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_15348</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HCE2"/>
    <dbReference type="OrthoDB" id="6781249at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033467">
        <property type="entry name" value="Tesmin/TSO1-like_CXC"/>
    </dbReference>
    <dbReference type="SMART" id="SM01114">
        <property type="entry name" value="CXC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tesmin/TSO1-like CXC" evidence="1">
        <location>
            <begin position="74"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8040904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="127" mass="14002" checksum="CE3F71608E75554F" modified="2023-09-13" version="1">MLFAKNGKEGRQLPPTFGTLKPHTERSFFMALVWKTSCKPCPCIPAPTEYSWKLVDGQLIPILCTNPPAPEALLELRRCSCRGSCGTNRCGCKRNLMACTDACECSDGCENTEDLTPEIEDDDELLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HDB0</accession>
    <name>A0A9Q1HDB0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein arginine N-methyltransferase</fullName>
            <ecNumber evidence="5">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_12754</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA).</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N-methyltransferase family. PRMT7 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="5"/>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HDB0"/>
    <dbReference type="OrthoDB" id="412876at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042054">
        <property type="term" value="F:histone methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016274">
        <property type="term" value="F:protein-arginine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000070">
        <property type="entry name" value="Protein arginine N-methyltransferase 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000071">
        <property type="entry name" value="Protein arginine N-methyltransferase 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.160.11">
        <property type="entry name" value="Hnrnp arginine n-methyltransferase1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025799">
        <property type="entry name" value="Arg_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014644">
        <property type="entry name" value="MeTrfase_PRMT7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055135">
        <property type="entry name" value="PRMT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11006">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11006:SF4">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06325">
        <property type="entry name" value="PrmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22528">
        <property type="entry name" value="PRMT_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036946">
        <property type="entry name" value="Arg_N-mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51678">
        <property type="entry name" value="SAM_MT_PRMT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein arginine N-methyltransferase" evidence="7">
        <location>
            <begin position="223"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="Protein arginine N-methyltransferase" evidence="7">
        <location>
            <begin position="574"/>
            <end position="715"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR036946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF22528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8041835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="726" mass="81466" checksum="00B73B1D39B04177" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HGV6</accession>
    <name>A0A9Q1HGV6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AP-5 complex subunit mu-1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Adaptor-related protein complex 5 subunit mu-1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_07294</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Probably part of the adaptor protein complex 5 (AP-5) a tetramer composed of AP5B1, AP5M1, AP5S1 and AP5Z1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endomembrane system</location>
            <topology evidence="7">Peripheral membrane protein</topology>
            <orientation evidence="7">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the adaptor complexes medium subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HGV6"/>
    <dbReference type="OrthoDB" id="1877176at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030119">
        <property type="term" value="C:AP-type membrane coat adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09256">
        <property type="entry name" value="AP_MuD_MHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1170">
        <property type="entry name" value="Mu homology domain, subdomain B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036168">
        <property type="entry name" value="AP2_Mu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039591">
        <property type="entry name" value="AP5M1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028565">
        <property type="entry name" value="MHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16082">
        <property type="entry name" value="AP-5 COMPLEX SUBUNIT MU-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16082:SF2">
        <property type="entry name" value="AP-5 COMPLEX SUBUNIT MU-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00928">
        <property type="entry name" value="Adap_comp_sub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49447">
        <property type="entry name" value="Second domain of Mu2 adaptin subunit (ap50) of ap2 adaptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51072">
        <property type="entry name" value="MHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="MHD" evidence="9">
        <location>
            <begin position="230"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8044521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="547" mass="60984" checksum="84C40CE1DCC43A50" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HH12</accession>
    <name>A0A9Q1HH12_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phospholipase B1, membrane-associated</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Lysophospholipase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Phospholipase A2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Phospholipase B/lipase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Triacylglycerol lipase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="44" type="ORF">HOLleu_07484</name>
    </gene>
    <organism evidence="44 45">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="44" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="44">Nanhai2018</strain>
            <tissue evidence="44">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="20">Calcium-independent membrane-associated phospholipase that catalyzes complete diacylation of phospholipids by hydrolyzing both sn-1 and sn-2 fatty acyl chains attached to the glycerol backbone (phospholipase B activity). Has dual phospholipase and lysophospholipase activities toward diacylphospholipids. Preferentially cleaves sn-2 ester bonds over sn-1 bonds. Acts as a lipase toward glycerolipid substrates. Hydrolyzes fatty acyl chains of diacylglycerols with preference for the sn-2 position and of triacylglycerols with not positional selectivity. May also hydrolyze long chain retinyl esters such as retinyl palmitate. May contribute to digestion of dietary phospholipids, glycerolipids and retinoids, facilitating lipid absorption at the brush border.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="32">
            <text>1,2,3-tri-(9Z-octadecenoyl)-glycerol + H2O = di-(9Z)-octadecenoylglycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38575"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:53753"/>
            <dbReference type="ChEBI" id="CHEBI:75945"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="32">
            <dbReference type="Rhea" id="RHEA:38576"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + H2O = 1-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40923"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28610"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:74669"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="28">
            <dbReference type="Rhea" id="RHEA:40924"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="40">
            <text>1,2-dihexadecanoyl-sn-glycero-3-phosphocholine + 2 H2O = sn-glycerol 3-phosphocholine + 2 hexadecanoate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:40975"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:72999"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="40">
            <dbReference type="Rhea" id="RHEA:40976"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>1,2-dihexadecanoyl-sn-glycero-3-phosphocholine + H2O = 1-hexadecanoyl-sn-glycero-3-phosphocholine + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41223"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:72998"/>
            <dbReference type="ChEBI" id="CHEBI:72999"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="29">
            <dbReference type="Rhea" id="RHEA:41224"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="41">
            <text>1,3-di-(9Z-octadecenoyl)-glycerol + H2O = 1-(9Z-octadecenoyl)-glycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:39939"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:75342"/>
            <dbReference type="ChEBI" id="CHEBI:75735"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="41">
            <dbReference type="Rhea" id="RHEA:39940"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="37">
            <text>1,3-dihexadecanoyl-2-(9Z-octadecenoyl)glycerol + H2O = 1,3-dihexadecanoylglycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40983"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:75688"/>
            <dbReference type="ChEBI" id="CHEBI:77619"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="37">
            <dbReference type="Rhea" id="RHEA:40984"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>1,3-dihexadecanoyl-2-(9Z-octadecenoyl)glycerol + H2O = 1-hexadecanoyl-2-(9Z-octadecenoyl)-glycerol + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40979"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:75585"/>
            <dbReference type="ChEBI" id="CHEBI:75688"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:40980"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>1-(9Z-octadecenoyl)-glycerol + H2O = glycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38487"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:75342"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:38488"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="38">
            <text>1-O-hexadecyl-2-(9Z)-octadecenoyl-sn-glycero-3-phosphocholine + H2O = 1-O-hexadecyl-sn-glycero-3-phosphocholine + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40915"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:34112"/>
            <dbReference type="ChEBI" id="CHEBI:64496"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="38">
            <dbReference type="Rhea" id="RHEA:40916"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>1-hexadecanoyl-2-(9Z)-octadecenoyl-3-octadecanoyl-sn-glycerol + H2O = 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycerol + octadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41111"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:25629"/>
            <dbReference type="ChEBI" id="CHEBI:75466"/>
            <dbReference type="ChEBI" id="CHEBI:77623"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:41112"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="39">
            <text>1-hexadecanoyl-2-(9Z)-octadecenoyl-3-octadecanoyl-sn-glycerol + H2O = 1-hexadecanoyl-3-octadecanoyl-sn-glycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41103"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:77623"/>
            <dbReference type="ChEBI" id="CHEBI:77624"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="39">
            <dbReference type="Rhea" id="RHEA:41104"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>1-hexadecanoyl-2-(9Z)-octadecenoyl-3-octadecanoyl-sn-glycerol + H2O = 2-(9Z-octadecenoyl)-3-octadecanoyl-sn-glycerol + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41107"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:75558"/>
            <dbReference type="ChEBI" id="CHEBI:77623"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:41108"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine + H2O = (9Z,12Z)-octadecadienoate + 1-hexadecanoyl-sn-glycero-3-phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40811"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30245"/>
            <dbReference type="ChEBI" id="CHEBI:72998"/>
            <dbReference type="ChEBI" id="CHEBI:73002"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:40812"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="30">
            <text>1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine + H2O = 2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40971"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:73002"/>
            <dbReference type="ChEBI" id="CHEBI:76084"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="30">
            <dbReference type="Rhea" id="RHEA:40972"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phospho-(1'-sn-glycerol) + H2O = 1-hexadecanoyl-sn-glycero-3-phospho-(1'-sn-glycerol) + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40919"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:72841"/>
            <dbReference type="ChEBI" id="CHEBI:75158"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:40920"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="36">
            <text>1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + H2O = 1-hexadecanoyl-sn-glycero-3-phosphocholine + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38779"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:72998"/>
            <dbReference type="ChEBI" id="CHEBI:73001"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="36">
            <dbReference type="Rhea" id="RHEA:38780"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="34">
            <text>1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine + H2O = 1-hexadecanoyl-sn-glycero-3-phosphoethanolamine + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40911"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:73004"/>
            <dbReference type="ChEBI" id="CHEBI:73007"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="34">
            <dbReference type="Rhea" id="RHEA:40912"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="35">
            <text>1-hexadecanoyl-sn-glycero-3-phosphocholine + H2O = sn-glycerol 3-phosphocholine + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40435"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:72998"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="35">
            <dbReference type="Rhea" id="RHEA:40436"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="31">
            <text>1-octadecanoyl-2-(9Z,12Z)-octadecadienoyl-sn-glycerol + H2O = 1-octadecanoyl-sn-glycerol + (9Z,12Z)-octadecadienoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40927"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30245"/>
            <dbReference type="ChEBI" id="CHEBI:75550"/>
            <dbReference type="ChEBI" id="CHEBI:77097"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="31">
            <dbReference type="Rhea" id="RHEA:40928"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>2,3-di-(9Z)-octadecenoyl-sn-glycerol + H2O = 3-(9Z-octadecenoyl)-sn-glycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:75824"/>
            <dbReference type="ChEBI" id="CHEBI:75938"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:42605"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="42">
            <text>2-(9Z-octadecenoyl)-glycerol + H2O = glycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38491"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:73990"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="42">
            <dbReference type="Rhea" id="RHEA:38492"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1-acyl-sn-glycero-3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15801"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.4"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:15802"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>a 1-O-alkyl-2-acyl-sn-glycero-3-phosphocholine + H2O = a 1-O-alkyl-sn-glycero-3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36231"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:30909"/>
            <dbReference type="ChEBI" id="CHEBI:36702"/>
            <dbReference type="EC" id="3.1.1.4"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:36232"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="33">
            <text>a 1-acyl-sn-glycero-3-phosphocholine + H2O = sn-glycerol 3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15177"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.5"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="33">
            <dbReference type="Rhea" id="RHEA:15178"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12044"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17855"/>
            <dbReference type="ChEBI" id="CHEBI:18035"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="EC" id="3.1.1.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:12045"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 'GDSL' lipolytic enzyme family. Phospholipase B1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="44">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HH12"/>
    <dbReference type="OrthoDB" id="10265800at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031526">
        <property type="term" value="C:brush border membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050253">
        <property type="term" value="F:retinyl-palmitate esterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01824">
        <property type="entry name" value="Phospholipase_B_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1110:FF:000005">
        <property type="entry name" value="Phospholipase B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035547">
        <property type="entry name" value="Phospholipase_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038885">
        <property type="entry name" value="PLB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21325:SF31">
        <property type="entry name" value="GH22081P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21325">
        <property type="entry name" value="PHOSPHOLIPASE B, PLB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="45" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 43" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="43">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040276856" description="Phospholipase B1, membrane-associated" evidence="43">
        <location>
            <begin position="20"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00029723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00031182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00031485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00033022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00045916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00047324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00047438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00047459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00048011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00048015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00048049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00048058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00048227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00048362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00048374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00048386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="ARBA" id="ARBA00048454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="ARBA" id="ARBA00048613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="35">
        <source>
            <dbReference type="ARBA" id="ARBA00048656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="36">
        <source>
            <dbReference type="ARBA" id="ARBA00048699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="37">
        <source>
            <dbReference type="ARBA" id="ARBA00048869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="38">
        <source>
            <dbReference type="ARBA" id="ARBA00048872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="39">
        <source>
            <dbReference type="ARBA" id="ARBA00048939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="40">
        <source>
            <dbReference type="ARBA" id="ARBA00049363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="41">
        <source>
            <dbReference type="ARBA" id="ARBA00049372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="42">
        <source>
            <dbReference type="ARBA" id="ARBA00049461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="43">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="44">
        <source>
            <dbReference type="EMBL" id="KAJ8044686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="45">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="419" mass="47502" checksum="E1B92E7323DA46B4" modified="2023-09-13" version="1">MYSILLFVIVTVISVIVEGEPIPNDDPWEQYTEYLRGLNEDHHLRFDEEFPFPDCSKYNNKFEFTCEIERSEEPPTSAHKVRPGDIDVIGAVGDSLTAANGAGACFLPAVIRQYRGVSWSAGGNDEYEEALTLPNILKKFNPQLRGYSTGYGNVNSKGSHFNCAVPAARSEKMPEQTGNLIDRLRSSTEIDFEKDWKLITLFVGGNDLCQYCKDRERSKAENYVSKIRDALDIMMDQVLPRTIVNVVGVMRVPEIVSLVSVKCNLIHSILCPCSTDITPEEEFEFVELTLEYQVLLEALILSGRYDKREDFTVVYQPFLTETDLPKDEFGEPDKTYFAPDCFHLGIKGHAIAAKELWNNMMEPVGSKRTNWDFDEPLLCPTKDYPYIFTNNNSVPQTLKSPLKSSKKDSISEKLLKILN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HJ48</accession>
    <name>A0A9Q1HJ48_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">P2X purinoceptor 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_01259</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HJ48"/>
    <dbReference type="OrthoDB" id="5945976at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046815">
        <property type="entry name" value="P2RX7_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36981:SF3">
        <property type="entry name" value="UBIQUITIN-LIKE PROTEASE FAMILY PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36981">
        <property type="entry name" value="ZGC:195170"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20478">
        <property type="entry name" value="P2RX7_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="P2X purinoreceptor 7 intracellular" evidence="1">
        <location>
            <begin position="46"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF20478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8048794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="193" mass="21662" checksum="F74BEF758C9D8224" modified="2023-09-13" version="1">MTSNLFSLWKNCEREKDLVNLTWKSDDSDDDDYAGLGLVVQGADRMNNTAWCRCSNCNAMPSQLECKCCTEVAAVRRVVPNLEAGQCIISHLDFNIICLHKQVLRATIIMRNDVRGHVVNVPQGLENKTYRYAAYRMFTYWVHGKLGQGVRKVVPACAVSKIRETFPDPNGVYVGFQIGDDGVEVEVDEVQAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HJN9</accession>
    <name>A0A9Q1HJN9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Histamine N-methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_06599</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HJN9"/>
    <dbReference type="OrthoDB" id="5984880at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13489">
        <property type="entry name" value="Methyltransf_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8047566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="328" mass="37430" checksum="71293A6BB29FF52D" modified="2023-09-13" version="1">MAFYEISTVLRFPSNEDKMRTLKEHQYHRSLEEFLRRCDQEQVMLKWIQTNFHKILHMIPAATPSETTRFIGVGCGNGIMDVEMLKMASKFFPRLHNIGIDPNDEALTIFKQRVKKEPLLDKVNVELFRETAERFQPVTSNATKKDSSRSQFTHMLHMLYHVKSIEDAVQKYYDLLGDGGVLLISLASRANNELYNTFKKFETLQSNQTKSSAVERNDKRYSDDVIKVLEKLGYHYTVENVGYNLDVSGCLESKTEGGKRLLDFVCLTNAAQSYETLSEENQRVLIECLKECCVTSADGKNSVRLTFDIITVVKPMGGVVGNQSNGII</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0Y8Z0</accession>
    <name>A0A9Q0Y8Z0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Immunoglobulin superfamily member 10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_44218</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000717">
        <property type="protein sequence ID" value="KAJ8018022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0Y8Z0"/>
    <dbReference type="OrthoDB" id="6353782at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071222">
        <property type="term" value="P:cellular response to lipopolysaccharide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013151">
        <property type="entry name" value="Immunoglobulin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051713">
        <property type="entry name" value="T-cell_Activation_Regulation"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25466:SF9">
        <property type="entry name" value="FIBRONECTIN TYPE-III DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25466">
        <property type="entry name" value="T-LYMPHOCYTE ACTIVATION ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00047">
        <property type="entry name" value="ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040484893" evidence="11">
        <location>
            <begin position="25"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="197"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8018022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="479" mass="53140" checksum="A9C0E8536B9D3C3B" modified="2023-09-13" version="1">MNAERILLFLVCFLFLSFDVGAKAKDIGCTSPQYVSTGSEGVINCSFRKGFYSLLWYSSPDVFHSEAVLTMVGSVKSGPGYLSGEFDIYPNGSLIIYNVSLENEGTFTAIILETPTSDAIRYVVDVIITAPTLSRLPLILECRNEGRICFRILPLHTEVSCKIQDARPAISLHWMVRTNSGDRNVSSSLSITNGTHPFFTSLATTSALFTFSSTLALLVCKADSPSGLLENDESLILIQNGVETLSSVPSVIKYVERGSRLVLNCTVSKRLYFVWQVQKPLETVFSNILFAVFVEGNLQSHIEDHGYNLHSEGTLFIDDVGTEDEGTYRCISSNGMEDDLLQYDVTVFVIDGCEGQHYCVLEMEPEGDLTCVVRGIRPWVQLKIIVSDDQSSTAFLLYGQQSTTQSNGDVFDISLTSKYRLNDASKTRVTIECKVIGTDIEQLNLSTKLDILFLNNVYCFVSQCQLMKVECNKFSDVVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YAH8</accession>
    <name>A0A9Q0YAH8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_44413</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000858">
        <property type="protein sequence ID" value="KAJ8017906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YAH8"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8017906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="94" mass="10855" checksum="A2185AC8680E9D1A" modified="2023-09-13" version="1">MLSVDFGRKFSDHLDTVAPTKKTVVVNKVRASKYMDELHLLRTQLCRLERVWLYEPLMINKQIGHAARTAYHKERKMGKPNSTVHASSPLTPEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YBK4</accession>
    <name>A0A9Q0YBK4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_43451</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000314">
        <property type="protein sequence ID" value="KAJ8018521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YBK4"/>
    <dbReference type="OrthoDB" id="10034833at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.395.10">
        <property type="entry name" value="Adenoviral Proteinase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8018521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="172" mass="19865" checksum="388CE9E5A060BE23" modified="2023-09-13" version="1">MDSMQLTHVLRSDRFTRPYFQGVFPSDCLRRLKPELPCAVVVNTDLSHSPGAHWVAVFISKNGVGDFFDSYGLPPSTTADIEKFLNRHCVQRQNNNVSLQGLYSTVCGQYAVFFLLHRCRGIEMKQITGWFTRQPHSNDEMVNDFITRHFPCVNMPVYDLPFLKQLSTRRTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YC04</accession>
    <name>A0A9Q0YC04_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_43321</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000268">
        <property type="protein sequence ID" value="KAJ8018600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YC04"/>
    <dbReference type="OrthoDB" id="409273at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8018600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="171" mass="19023" checksum="5D64F630B9972356" modified="2023-09-13" version="1">MVECGATVSKVDPAVFYWVDSSDKVYGILACHVDDFIWGGDQEFEDIISKIKSTFKVGKEIDDSFKYCGIDLLCDDNVIYLSQDSYTDGLSIIDISATRSVDKSAKLTAEEGHVLRSKVGQLLWLAHQSRPDLLFDVTKIANNLNKGSVGDILDIHIILCKAKNSNLRLKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YDK5</accession>
    <name>A0A9Q0YDK5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Steroid 21-hydroxylase</fullName>
            <ecNumber evidence="14">1.14.14.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">21-OHase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Cytochrome P-450c21</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Cytochrome P450 21</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Cytochrome P450 XXI</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Cytochrome P450-C21</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">HOLleu_40500</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="24">Nanhai2018</strain>
            <tissue evidence="24">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="21">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 22">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.14.16" evidence="14"/>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YDK5"/>
    <dbReference type="OrthoDB" id="1055148at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004509">
        <property type="term" value="F:steroid 21-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008202">
        <property type="term" value="P:steroid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006805">
        <property type="term" value="P:xenobiotic metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000049">
        <property type="entry name" value="steroid 21-hydroxylase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050182">
        <property type="entry name" value="Cytochrome_P450_fam2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300:SF403">
        <property type="entry name" value="CYTOCHROME P450 306A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300">
        <property type="entry name" value="CYTOCHROME P450 508A4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 21 22" id="KW-0349">Heme</keyword>
    <keyword evidence="10 21 22" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="13 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 21 22" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0492">Microsome</keyword>
    <keyword evidence="11 22" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="9 22" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="23" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="21">
        <location>
            <position position="440"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00044040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00044116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00044217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00044265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00044282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00044304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00044342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="KAJ8020812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="495" mass="56460" checksum="10BCE2ACA975B3A1" modified="2023-09-13" version="1">MFDSGTVLIALIAVVITLVVVRYIKARRPVPNYPPGPVGLPIVGSLPQFLGREDYHRYFMELGEKYGSVCSYMFGHQSFVLVNGIDSIKEALVTRGNDIADRPENYVVTLYNPHQLGLFDARFNDIWFNQRHFAHKTLRGFGFGKTSFESKIMEEVEFLIKNFKATQQKPTDIRNFITNCVSNIICSITFGKRYNHFEDPEFLKAMTCLRDWFTTTSSLMLQLGHVYPIIRPLLWKQARDVKNATGSLEKFLLGEIAKKEEKLDTISEPGDFVESYLLELKKDTEGKFKKLYLKHDIMDIFAAGTETTSTTLTWSVLYMMVHPEVQRKVQKELDQVVGREKLPSWANKSDLPYTEATLLEIQRLSSLVPVVPHATLKPTTLGGYNLPAKCDVWLNLWSIHHDPKLYPDPEKFDPNRFLTEDGQVKKQESFMPFGAGRRVCMGESLAKLELFLFFSAMLHQFSFSVPEGTVAPSLEGDLGITIMPKPFEVVITERS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YG50</accession>
    <name>A0A9Q0YG50_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase/retrotransposon-derived protein RNase H-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_39408</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8022033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YG50"/>
    <dbReference type="OrthoDB" id="775972at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="CDD" id="cd09274">
        <property type="entry name" value="RNase_HI_RT_Ty3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000020">
        <property type="entry name" value="Transposon Tf2-6 polyprotein-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041577">
        <property type="entry name" value="RT_RNaseH_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF15">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17919">
        <property type="entry name" value="RT_RNaseH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase/retrotransposon-derived protein RNase H-like" evidence="1">
        <location>
            <begin position="98"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF17919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8022033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="241" mass="27951" checksum="7C8A14D95F58F1CF" modified="2023-09-13" version="1">MWILKKVSDRLTKYNVKLNKSKCELSKKELKFLGHRLTDHGIKINDKHHAITNATVTRDEKSLRSLLGLGGYFTKFIPNLTTVLEPMRQVLRQKRFLWTAEAMESFNLLKEMLCKAKVLSSFNQNLQTIITCDASDYGLGATLTQMKNGKEVTVQFTSRTLSESERKYSIVEKEALACVWACERWNTYLWGEFTLRTDQEALVTLFSKTSERQSLRLARWSFRLLKYNLRLNTSRGSSVLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YGZ7</accession>
    <name>A0A9Q0YGZ7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Growth hormone secretagogue receptor type 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_39871</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YGZ7"/>
    <dbReference type="OrthoDB" id="5962705at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24243">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24243:SF208">
        <property type="entry name" value="PYROKININ-1 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="102"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="180"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="223"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="271"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="341"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="381"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="10">
        <location>
            <begin position="120"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8020309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="442" mass="50209" checksum="6DDE2B17C03C9C12" modified="2023-09-13" version="1">MDVCSGATLSNVTEFATDDEIFDTPMSTVCAMDDRTFATFTSSFNDTAESETGDLMSLVTAFCFMSCNYTCPEYIYSVANDPTLVKDRLSVFMPDDHYSYSFIIPFVLVIAFLLLVGILGNALAIVVIVKNRVLQASSHYLVSLAVSDVLLLVLTGPTEIAANIHFWPWNYTRILCRLRYFLIEACAFTTVLHIAAFTIERYFVVCHPMKAKSLVSASRTKKIICFIWILSFVIAFPIFFAYDLLVACEGIPESTICEVKSDAWEARVQSFYIFSSIILFFIPMFLIIALYSLIARVMFGYNVKVSMRRSASMQKRKSKGSKKLKEKEDGIEKSRRQVVKILAFIAFCFFACWCPFHVIRLMPHFGSDGWSDGFLEFYHKWLYHISILLLYTSSVVNPILYNLMSKRFRRAVWATIRCRDAKYGTSVVCETRSTSVNGATSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YIG8</accession>
    <name>A0A9Q0YIG8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_40954</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8021164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YIG8"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040239915" evidence="1">
        <location>
            <begin position="25"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8021164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="182" mass="20052" checksum="69414D4A4BABB6F5" modified="2023-09-13" version="1">MFFLFSRVLLLVVGICACCCFGQGNVEGNNVVAALEEEKEGFQETAENDFERDQIRSELAVPIFGDTTEMDIISAILSFLQPSNSSEVDENSVVPTPVGGAQPPQPSEACTKDVEKFVEDLLASKEYALRMRTAYASIPYLDGIAFTYRLKYDGIYELCDGIKAANNGTPFNGQFCQLYIPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YIW5</accession>
    <name>A0A9Q0YIW5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Histone H2B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_35781</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the histone H2B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YIW5"/>
    <dbReference type="OrthoDB" id="1733721at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000558">
        <property type="entry name" value="Histone_H2B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23428">
        <property type="entry name" value="HISTONE H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00621">
        <property type="entry name" value="HISTONEH2B"/>
    </dbReference>
    <dbReference type="SMART" id="SM00427">
        <property type="entry name" value="H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="4">
        <location>
            <begin position="40"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8023365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="102" mass="11650" checksum="D50082F4E32115C6" modified="2023-09-13" version="1">MVGLEKLRVVSFLRRLLCHTASNAFSTSRKAAKAPRAPGDRKRKRTRRESYSIYIYKVIKQVHPDTGISSRAMSITNSFVDDIFEHITGEVTCLAHCNRNRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YJ60</accession>
    <name>A0A9Q0YJ60_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_35994</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ADP-ribosyl cyclase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YJ60"/>
    <dbReference type="OrthoDB" id="10028716at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061809">
        <property type="term" value="F:NAD+ nucleosidase activity, cyclic ADP-ribose generating"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016849">
        <property type="term" value="F:phosphorus-oxygen lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.82.10">
        <property type="entry name" value="ADP Ribosyl Cyclase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003193">
        <property type="entry name" value="ADP-ribosyl_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10912">
        <property type="entry name" value="ADP-RIBOSYL CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10912:SF7">
        <property type="entry name" value="ADP-RIBOSYL CYCLASE_CYCLIC ADP-RIBOSE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02267">
        <property type="entry name" value="Rib_hydrolayse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52309">
        <property type="entry name" value="N-(deoxy)ribosyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040488706" evidence="6">
        <location>
            <begin position="29"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8023530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="292" mass="32651" checksum="CFBF4229761BC60B" modified="2023-09-13" version="1">MASRNHNSYSTQLLQLFLSFMLAEVIISSNVAEEEILSKETFFERCRTASPGLRTGLTCEQLWAIFQRVFECPSSDCTPITDDSAYKEFVEQIPPTTPANRTLFYSGGTKVAAYNIGKKCIDKLTIQMTFLGQLFDDLKWCCVRNGSVDTQCSADDSCTSVTLNKFWTQASELFAKNATMHTAVILNGSGSAPPYREDSIFTTTEVPSLTSSVISMTAILVYENDQVKKRWTCSSPDSRLPDLHVALARKNIQYNCTDDYWYSSEQCSSANSLSMAIWMLPLILVIQKIIST</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YJ92</accession>
    <name>A0A9Q0YJ92_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Solute carrier family 22 member 6-B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_38436</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YJ92"/>
    <dbReference type="OrthoDB" id="3936150at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8021282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="155" mass="17628" checksum="E228BF870674255F" modified="2023-09-13" version="1">MDFEKVLKEVGHFGRYQKVQLVLLSASLLIAVLHLYMQVFAAGKGDHWCRSWKNEDCRDLNLTQKECEGIKKHISIPMKEGTSNQTENEFEQCVKYNVTKLNFKAAFEGNVTLMEKIPCDDGWVHDRSVFPSTIIIDVSISYGLFAYVSLASSIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YJJ6</accession>
    <name>A0A9Q0YJJ6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Helix-turn-helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_36289</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YJJ6"/>
    <dbReference type="OrthoDB" id="6782675at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058912">
        <property type="entry name" value="HTH_animal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21301:SF11">
        <property type="entry name" value="GIY-YIG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21301">
        <property type="entry name" value="REVERSE TRANSCRIPTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26215">
        <property type="entry name" value="HTH_animal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helix-turn-helix" evidence="2">
        <location>
            <begin position="154"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8023755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="289" mass="33248" checksum="BBDB4C4826A10E12" modified="2023-09-13" version="1">MNKEQYMDKMSDLIMEDKTYRLLKSDPTTSLENKIRKAIKELKEQNKLNKKPAKQLTPRNSLSPRIYGLPKVHKEGTPLRPIVSSINSPSYNLARHLAGILTPLSGKGISYIKNSQHFVERAKKISIETSDILVQKTDSKLSFSVYRKPTHTDQYLHFSSNHHVSAKINVVNTLLHRTLTLCDEAKVSDELDHITKALHKNDYPAHYVHRAVKKQTQQQITNKKASEEYNKGLTFMPYVKGVSERITRILNHAGVKTFYFRSQKLRDILSQPKDPLPKKLYCLCLLNPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YMU4</accession>
    <name>A0A9Q0YMU4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Synaptotagmin-15</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_38161</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8023082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YMU4"/>
    <dbReference type="OrthoDB" id="10259057at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070382">
        <property type="term" value="C:exocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017156">
        <property type="term" value="P:calcium-ion regulated exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00276">
        <property type="entry name" value="C2B_Synaptotagmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10024">
        <property type="entry name" value="SYNAPTOTAGMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10024:SF234">
        <property type="entry name" value="SYNAPTOTAGMIN-15-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="1">
        <location>
            <begin position="36"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="1">
        <location>
            <begin position="170"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8023082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="310" mass="35785" checksum="6C47AD255C2F2671" modified="2023-09-13" version="1">MEDLGQLRPELYQTDEKCAHERPKSGKKIEEVVEEFYGNTWFAVRFDRKESRLYIHLSKITDLPPRSQRAPTYDTFVNICLLPDEKHSFNSRIAKNNLNPPFEEVFTFNVDKSRGFENYTLRLSAYNADLPQWYNAIGHTLVPLADCVTPDEKPQDFKRKLSRKAKLSEDLGRILISLAYLPSLDRLSVVILRVHDIPLQKGLSEEYLETYVKVRLICGSEKIKTERTPSIKGTVNPVFAESLGYVIPAFFIERTVLVIQLMQKGHLRRDKLIGQVILGPYLLTDSKNLSHFGQMLAGREAVKKWHCLYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YN28</accession>
    <name>A0A9Q0YN28_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Endogenous retrovirus group K member 19 Pol protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_39211</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YN28"/>
    <dbReference type="OrthoDB" id="413361at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00024">
        <property type="entry name" value="CD_CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46585:SF1">
        <property type="entry name" value="CHROMO DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46585">
        <property type="entry name" value="INTEGRASE CORE DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="3">
        <location>
            <begin position="198"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="domain" description="Chromo" evidence="2">
        <location>
            <begin position="447"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8021886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="722" mass="84938" checksum="65938CA356F0C91C" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YPC4</accession>
    <name>A0A9Q0YPC4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cytochrome P450 4V2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_36937</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8024254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YPC4"/>
    <dbReference type="OrthoDB" id="1470350at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20628">
        <property type="entry name" value="CYP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050196">
        <property type="entry name" value="Cytochrome_P450_Monoox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291:SF189">
        <property type="entry name" value="CYTOCHROME P450 4C3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 6" id="KW-0349">Heme</keyword>
    <keyword evidence="5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="5">
        <location>
            <position position="457"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8024254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="511" mass="59140" checksum="8F62D2CAC1345A91" modified="2023-09-13" version="1">MEFAKMMQYVLGIVVVGFVSIFVRKICGNIWHRYKLRHVKGPRGVPFLGNILQFGNNNVDFFNAIENWCNEFREAGYFKLFIGFQPVVVVFNAYDMEKIFRDTSLDNKGFFYQLYVPWLGDGLLISKGSKWMTRRKLLTPSFHFSILTEFLPAFNDHAQTLTKKLCRCLDGSPLDITPLIALCSLDIICETIMGIKLGAQKGQGEDYVHAVHRLGDIAMLRMQYPWYWNEGLFRKMNIGREYVKCLDVVHQTTQKVIRERKMENKTKYKSDTIIDDETVDVRKRRRLAFLDLLIEVQKQNKDLTDEGIQEEVDTFMFEGHDTVSSSIAFAMYFIGRHPTVQHKIQDELHLVFGNDRDRTVTKEDLQKLEYLSCVMKESQRMLTTVPMIGRQLETDMNACGTTIPKGTIFFIASYWLHRDSKQFSNPDTFDPDRFLPTNSEGRHNFAFVPFSAGHRNCIGQKFASMEQKVILATLLRKVELTSTQDVTEMLLFQAPVLRSAKGIKMVVTPRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YQW4</accession>
    <name>A0A9Q0YQW4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_32037</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YQW4"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8027023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="98" mass="11603" checksum="48D691DBE220CE9B" modified="2023-09-13" version="1">MLCNHLLSKCKRVNFTLRRLPTVTTLCFEWNSTLPGVVIHSFGVNYHSQQSEIFTRRKSGHCRQSSKSEIHSFTFREFTFEANDIVRRVFRIHGDLDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YR10</accession>
    <name>A0A9Q0YR10_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Radial spoke head protein 3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_31871</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Flagellum axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the flagellar radial spoke RSP3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YR10"/>
    <dbReference type="OrthoDB" id="313308at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009290">
        <property type="entry name" value="Radial_spoke_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21648">
        <property type="entry name" value="FLAGELLAR RADIAL SPOKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21648:SF0">
        <property type="entry name" value="RADIAL SPOKE HEAD PROTEIN 3 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06098">
        <property type="entry name" value="Radial_spoke_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0969">Cilium</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0282">Flagellum</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="216"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="327"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="330"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="352"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8026908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="384" mass="44156" checksum="A9B0D1A41FC8FDD7" modified="2023-09-13" version="1">MTTVLPQKSGTYTFSSQPRTVPQRKKYRDPLAQQNQAPAPYGNIMYDRRIVRGNTYAQHTLPATAQPDPIEIQRQQEIRRRKIAAKRAKAQLRPASPEPVEGRKHIDVQTELYLEELSDRVEEADVDCQTDAFLDRPPTPLFIPAKTGMDVATQIYEGDLFDFEIEVKPILEVLVGKTVEQALLEVMEEEELANLRAQQREFEELRNAELVETQRLEEQERRHREEKERRMKQQRETLKKERETADKIAARAFAQSYLADLIPSVFGTLNDNGYFFDPVERDVELGFMPWIMEEVEKKIDMSVLGRTVLDAIIRDVVMQRSAAYFKLEESQQEPEEETTTQQPAEEEAVTEEAEKTEEKPEGDAGDEAAAETTEPPAEEEEGDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YSI6</accession>
    <name>A0A9Q0YSI6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_34790</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YSI6"/>
    <dbReference type="OrthoDB" id="10057935at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027901">
        <property type="entry name" value="CFAP90"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34444:SF3">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34444">
        <property type="entry name" value="LOC361192"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15074">
        <property type="entry name" value="CFAP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8024781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="171" mass="19517" checksum="DB4A09B68DA79642" modified="2023-09-13" version="1">MASQGKRMSNYGLKNPTPEESKLLWKEIVEREKKIRANHLAIKGDGSSAEWPGIKHLLPTKEEMEANNNYFNNKKQHGSSSTYDRLFHVKEGYQCKLHRDDREHTIGLDVHGEEAQKAIPVLSSSAYGHRVALEGPSRQHARIERVMKGFYRTRGTNLPTFGTDDQMDCSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BB04</accession>
    <name>A0A9Q1BB04_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_38428</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BB04"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8021276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="117" mass="12685" checksum="DF1377953DFD9525" modified="2023-09-13" version="1">MPHIERKNPIVFGGGQRSSGVTGGQTPKTLLTQYLEMRYGKRKNPIVCGGGQRSSGVIGGQTLKTLLTRYLKMRYAKRKDPIIFGGGQRSSGVTGGQTLKTLLTGYLKVGSLDKFHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BB89</accession>
    <name>A0A9Q1BB89_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">oleoyl-[acyl-carrier-protein] hydrolase</fullName>
            <ecNumber evidence="1">3.1.2.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_42629</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.14" evidence="1"/>
    <dbReference type="EMBL" id="JAIZAY010000102">
        <property type="protein sequence ID" value="KAJ8019038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="329835at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004312">
        <property type="term" value="F:fatty acid synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016297">
        <property type="term" value="F:fatty acyl-[ACP] hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05195">
        <property type="entry name" value="enoyl_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000209">
        <property type="entry name" value="Polyketide synthase Pks12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.110">
        <property type="entry name" value="Polyketide synthase dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049391">
        <property type="entry name" value="FAS_pseudo-KR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057326">
        <property type="entry name" value="KR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042104">
        <property type="entry name" value="PKS_dehydratase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013968">
        <property type="entry name" value="PKS_KR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050091">
        <property type="entry name" value="PKS_NRPS_Biosynth_Enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001031">
        <property type="entry name" value="Thioesterase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43775">
        <property type="entry name" value="FATTY ACID SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43775:SF7">
        <property type="entry name" value="FATTY ACID SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21149">
        <property type="entry name" value="FAS_pseudo-KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08659">
        <property type="entry name" value="KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00975">
        <property type="entry name" value="Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00822">
        <property type="entry name" value="PKS_KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="7" id="KW-0521">NADP</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="13">
        <location>
            <begin position="561"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="domain" description="Ketoreductase" evidence="12">
        <location>
            <begin position="896"/>
            <end position="1053"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ8019038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1471" mass="164362" checksum="E2D5AE72722301FE" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BB94</accession>
    <name>A0A9Q1BB94_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_39880</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BB94"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8020315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="140" mass="15747" checksum="D580DE51F6982301" modified="2023-09-13" version="1">MSKQISSIVKTYNFHLQNISRVCKYLTTKACQPAVQGLVVSQMDYCCSLLSGAPINQIQSLQRVQNNSARIISRVGHSNRISSVLAALHWLPCDLRLKFRILTYVHVRGFISFHQVISLQELFTSTSITGLSDHPLIHPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BBR1</accession>
    <name>A0A9Q1BBR1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_41259</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8019608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BBR1"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8019608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="104" mass="11928" checksum="8C33BFEBE0E9E47B" modified="2023-09-13" version="1">MTCSRIYPSAVQQPSPQHLSPENKLKSTFLYWAFKRFFICFTVDFFQYLWIQLLSFSLDEDLPLMVDVVSVSYPKAAIASRLNRPGRQSCCWIPASISGQENVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BC54</accession>
    <name>A0A9Q1BC54_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_02423</name>
        <name evidence="1" type="ORF">HOLleu_41523</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8019789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BC54"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8019789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8049610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="64" mass="7162" checksum="5F6E94A8D5DE7BB7" modified="2023-09-13" version="1">MESSREFNFNAAFLELSRSLALSLFARGTSSYATARCRSTTVPSSTCMHYQPLDQTRQILIKGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BDU2</accession>
    <name>A0A9Q1BDU2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DDE Tnp4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_40735</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BDU2"/>
    <dbReference type="OrthoDB" id="7903616at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027806">
        <property type="entry name" value="HARBI1_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13359">
        <property type="entry name" value="DDE_Tnp_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE Tnp4" evidence="3">
        <location>
            <begin position="63"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8020987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="167" mass="19405" checksum="C931679AA546DBAB" modified="2023-09-13" version="1">MEVFLRCIGDPGFQVGVGKDIGIHQSTVSRTFADVLDKVVAKADTWIQFPSDPTDFQAAKDAWQKTSYNKCHKKERLIIERCFGQLKRRFPMLQGRVRIQLRKVPSLIVACCIMHNVAKCLQDPNDFPEYNELYVDNNDAVHEDDRIRQRGQERRRQIANIIHENHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BDY3</accession>
    <name>A0A9Q1BDY3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Reverse transcriptase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_36926</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8024246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10064731at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09274">
        <property type="entry name" value="RNase_HI_RT_Ty3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000115">
        <property type="entry name" value="Polyprotein of retroviral origin, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.370:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 17.6-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000032">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 297-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon gypsy-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054465">
        <property type="entry name" value="Integrase_p58-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041373">
        <property type="entry name" value="RT_RNaseH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22938">
        <property type="entry name" value="Integrase_p58_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17917">
        <property type="entry name" value="RT_RNaseH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="10">
        <location>
            <begin position="819"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase" evidence="9">
        <location>
            <begin position="1233"/>
            <end position="1410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="314"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="588"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="652"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="78"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="314"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="329"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="353"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="596"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="621"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="668"/>
            <end position="692"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8024246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1631" mass="186280" checksum="89194E69951BAC4E" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BH24</accession>
    <name>A0A9Q1BH24_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Transcriptional repressor NF-X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_34933</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NFX1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BH24"/>
    <dbReference type="OrthoDB" id="6512771at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06008">
        <property type="entry name" value="NF-X1-zinc-finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034078">
        <property type="entry name" value="NFX1_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000967">
        <property type="entry name" value="Znf_NFX1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12360">
        <property type="entry name" value="NUCLEAR TRANSCRIPTION FACTOR, X-BOX BINDING 1 NFX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12360:SF12">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR NF-X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01422">
        <property type="entry name" value="zf-NF-X1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00438">
        <property type="entry name" value="ZnF_NFX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="12">
        <location>
            <begin position="395"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="13">
        <location>
            <begin position="398"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="10"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="27"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="60"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="102"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="127"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="149"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="200"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="271"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="334"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ8024889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="632" mass="70512" checksum="8F0FCA85FC530BFE" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BHI3</accession>
    <name>A0A9Q1BHI3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Frizzled-4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HOLleu_31790</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Nanhai2018</strain>
            <tissue evidence="18">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor Fz/Smo family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BHI3"/>
    <dbReference type="OrthoDB" id="5959102at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042813">
        <property type="term" value="F:Wnt receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017147">
        <property type="term" value="F:Wnt-protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035567">
        <property type="term" value="P:non-canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.2000.10:FF:000037">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000020">
        <property type="entry name" value="Frizzled class receptor 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.2000.10">
        <property type="entry name" value="Frizzled cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015526">
        <property type="entry name" value="Frizzled/SFRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000539">
        <property type="entry name" value="Frizzled/Smoothened_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020067">
        <property type="entry name" value="Frizzled_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036790">
        <property type="entry name" value="Frizzled_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11309">
        <property type="entry name" value="FRIZZLED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11309:SF23">
        <property type="entry name" value="FRIZZLED-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01534">
        <property type="entry name" value="Frizzled"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01392">
        <property type="entry name" value="Fz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00489">
        <property type="entry name" value="FRIZZLED"/>
    </dbReference>
    <dbReference type="SMART" id="SM00063">
        <property type="entry name" value="FRI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01330">
        <property type="entry name" value="Frizzled"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63501">
        <property type="entry name" value="Frizzled cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50038">
        <property type="entry name" value="FZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="9 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040498295" evidence="15">
        <location>
            <begin position="30"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="228"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="262"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="313"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="351"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="393"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="441"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="483"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="domain" description="FZ" evidence="16">
        <location>
            <begin position="38"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="17">
        <location>
            <begin position="226"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="162"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="178"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="43"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="51"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="88"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="115"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="119"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ8026840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="543" mass="61663" checksum="EA7FDDE4D4BFBA9F" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BJ81</accession>
    <name>A0A9Q1BJ81_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small ribosomal subunit protein uS9m</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">28S ribosomal protein S9, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_32695</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the universal ribosomal protein uS9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BJ81"/>
    <dbReference type="OrthoDB" id="10254627at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000035">
        <property type="entry name" value="28S ribosomal protein S9, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000754">
        <property type="entry name" value="Ribosomal_uS9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023035">
        <property type="entry name" value="Ribosomal_uS9_bac/plastid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020574">
        <property type="entry name" value="Ribosomal_uS9_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001099">
        <property type="entry name" value="PRK00132.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21569">
        <property type="entry name" value="RIBOSOMAL PROTEIN S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21569:SF1">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US9M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00380">
        <property type="entry name" value="Ribosomal_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00360">
        <property type="entry name" value="RIBOSOMAL_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 9 11" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="281"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="287"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00076042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8027530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="306" mass="35425" checksum="DBBAE7C035A8BFA9" modified="2023-09-13" version="1">MMGEDAENFSQEDIDRSISYLLPSGLFEKKARPMLRPPKDYYPPQKAAQFDNDGRPFSFLFYTGKPNYYNLMHEAADWLNKVIQQEDALIQRGESLDGIPQVNLARSEWIGKLALEKIVIEKLKDNEYERFINLLERILKQPFAIEAQEFIMKFRQKIEAVLSKEVIEPLQKDENGRFYSRGSARRKSSNASVILTDGGSGKITINGEEFLRTFYQLCHREDILFPLHFANMLDRFDIECTTEGGGPSGQSGAIRLAISKALRSFVDEEFVEKMRQAGLLTKDPRIKERKKPGQKRARKKFTWKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BJH2</accession>
    <name>A0A9Q1BJH2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_29776</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8027737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BJH2"/>
    <dbReference type="OrthoDB" id="10068564at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8027737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="115" mass="13126" checksum="35DB96D952D18D6A" modified="2023-09-13" version="1">MKHYAEKKHSLKQSSIRVGDSVLLRQNKINKLTPPYDPKPFKVISVKGSTITAEGDGRTITRNSSFFKVFNFAEEEEEMDQLDIHNGKHSTTQEGQSGYNLRSSRKLTSRLIEEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BKA3</accession>
    <name>A0A9Q1BKA3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Eukaryotic translation initiation factor 5B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_30381</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. IF-2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BKA3"/>
    <dbReference type="OrthoDB" id="4928at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01887">
        <property type="entry name" value="IF2_eIF5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000112">
        <property type="entry name" value="Eukaryotic translation initiation factor 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015760">
        <property type="entry name" value="TIF_IF2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381:SF4">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR IF-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 8" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="7">
        <location>
            <begin position="597"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="399"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="515"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="15"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="52"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="72"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="82"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="128"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="156"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="168"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="205"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="262"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="278"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="298"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="464"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="523"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="573"/>
            <end position="582"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8028210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="878" mass="99194" checksum="252C4DDE15F604A4" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BKT1</accession>
    <name>A0A9Q1BKT1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_30579</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BKT1"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8028371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="207" mass="23192" checksum="286B9D44974A8666" modified="2023-09-13" version="1">MAASTNPSAISVTLSQIHRQIEQMVATRRPSCPTWKCNCKIVYNCGSNTGFPEDGDADTGCLKDLASYSYPMNIAGKRWRRNLWNSQQYCQRECERRLHAFLCLDDDGDFTPYSYGSICAQLILGAIIPVTGGTSVPCCETYLEWGPKWCEDSWYRPLENLHCYIRDLSGLPSGSFAFGSETCSSVDDTPQFNWCTNDPTCTFVPVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BLK8</accession>
    <name>A0A9Q1BLK8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Fucosyltransferase</fullName>
            <ecNumber evidence="12">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_28163</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Golgi apparatus</location>
            <location evidence="12">Golgi stack membrane</location>
            <topology evidence="12">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the glycosyltransferase 10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="12"/>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8028907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BLK8"/>
    <dbReference type="OrthoDB" id="427096at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008417">
        <property type="term" value="F:fucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11660:FF:000002">
        <property type="entry name" value="Alpha-(1,3)-fucosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11660">
        <property type="entry name" value="Glycosyl transferase family 10, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055270">
        <property type="entry name" value="Glyco_tran_10_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001503">
        <property type="entry name" value="Glyco_trans_10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038577">
        <property type="entry name" value="GT10-like_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48438">
        <property type="entry name" value="ALPHA-(1,3)-FUCOSYLTRANSFERASE C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48438:SF1">
        <property type="entry name" value="ALPHA-(1,3)-FUCOSYLTRANSFERASE C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00852">
        <property type="entry name" value="Glyco_transf_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9 12" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Fucosyltransferase C-terminal" evidence="13">
        <location>
            <begin position="231"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ8028907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="460" mass="52392" checksum="A6E55E7A3D8AC801" modified="2023-09-13" version="1">MRCTFTPERNEQRNVYHKRNPSVQTDSSRGEYNTSFTRVAADGRFQEVSSNVTPVVERLVSNKLEPASLNFSDDLHTSIEPKKELPKEFEFQLKILIFSNSYYILSYSHSRVTTCATGKVLARYADYDPEDVTSAYDVIVFGGDIDNDRLWDFLSKYRNMHQLWLLNTDLNPITTKHIIPPMTSSTTAFNISLTYHSKSEISVPYGYCLRRTNNTSQVNETLSQNYLELHPKLVAWYSGDCHNVSWNRQRFVSDLSRSIPIDLYGTCGNLSCGGPTLLSKYRRGSAYCRSLSEYKFVLALEESCCSEYITDNFWNVLALYNGIPVVVGPSKDQYERLAPPNSFIFAGDFDSTSELAAYMRKVASDARIYNSFFKWKQHWSVGMNSAKNRLLPAGNVQCSLLEYIQGRTVNSTKPIPITTQVDPYGPYWIGSCTSCEEHSMLEKYRLVDTDGHFAGVRSDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BLZ6</accession>
    <name>A0A9Q1BLZ6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Rho guanine nucleotide exchange factor 18</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_28281</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BLZ6"/>
    <dbReference type="OrthoDB" id="28045at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035023">
        <property type="term" value="P:regulation of Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13329">
        <property type="entry name" value="PH_RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.900.10:FF:000004">
        <property type="entry name" value="Rho guanine nucleotide exchange factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041020">
        <property type="entry name" value="PH_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051632">
        <property type="entry name" value="Rho_GEF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13944">
        <property type="entry name" value="AGAP007712-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13944:SF21">
        <property type="entry name" value="CYSTS, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17838">
        <property type="entry name" value="PH_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DH" evidence="11">
        <location>
            <begin position="94"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="10">
        <location>
            <begin position="328"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="559"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="755"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="838"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="878"/>
            <end position="973"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="35"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="559"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="608"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="640"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="682"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="755"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="889"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="942"/>
            <end position="958"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8029004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="973" mass="109204" checksum="3CE7682A707D35EF" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BPW2</accession>
    <name>A0A9Q1BPW2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">WD repeat-containing protein 73</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_27074</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BPW2"/>
    <dbReference type="OrthoDB" id="9822052at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042795">
        <property type="entry name" value="Wdr73"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46947">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 73"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46947:SF1">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 73"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8030611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="270" mass="30204" checksum="D2A33901D60DEDCA" modified="2023-09-13" version="1">MSVEPEDDWNWMIQSMKKYAHLHMYDLSQPTSVLEWMDQKTIFVADSSSPNGSYEMLLLSLPLKLTNSADDQLKLSKDRDFKIVCGGFHQQPVHAMAMVSDQLLLTSSPSDTDEIILWEIKEDEDVITQLKTHQGQRSGMSNAPFVAANPRTDAQVARGSFLENLVVVNLETGKDLWSYEVEDKPCRQQLASIDYIDDCNLLVCNASNGRLRIIDTRSPSSNCSSSQPSSSSIQLSLTSSVSLKPATWSCSVNFHSRHRRVGTDLKDGSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BQE4</accession>
    <name>A0A9Q1BQE4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cilia- and flagella-associated protein 300</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_27800</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Cilium- and flagellum-specific protein that plays a role in axonemal structure organization and motility. May play a role in outer and inner dynein arm assembly.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CFAP300 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8031158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BQE4"/>
    <dbReference type="OrthoDB" id="10259249at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029416">
        <property type="entry name" value="CFAP300"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31078">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31078:SF1">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14926">
        <property type="entry name" value="CFAP300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8031158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="265" mass="31022" checksum="02D58D4A8D8E744B" modified="2023-09-13" version="1">MAAAVKREKKFNFLHLNEKKCAFLNDKDVQDLFMKWSMQGNICVQYYSYDQLFQLYQKSDFVLDFLQDPVVTSSLQVITDSGSSGPVGISAVKVEVTEIPCSITSMEFFDRLSTDGIVRDSGHIVKCFDDFYEDFTISDELRKMLLMENSDNYEIYSDKEREEFLFRLFQHICLGGAVCQYEDFIEPYLKTTKMIYKDLVSVRKDPDTKELKVSSIVIKVKGWDKDDFQFYPSDQDHEQTFAYFIIDPLKRHVAVLYHKWGRGVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BRJ0</accession>
    <name>A0A9Q1BRJ0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">RNA-binding motif protein, X-linked 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_27941</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8031264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BRJ0"/>
    <dbReference type="OrthoDB" id="2573941at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12411">
        <property type="entry name" value="RRM_ist3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000962">
        <property type="entry name" value="RBMX2 ortholog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051847">
        <property type="entry name" value="RNA_proc/Spliceosome_comp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045844">
        <property type="entry name" value="RRM_Ist3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45880">
        <property type="entry name" value="RNA-BINDING MOTIF PROTEIN, X-LINKED 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45880:SF1">
        <property type="entry name" value="RNA-BINDING MOTIF PROTEIN, X-LINKED 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="36"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="136"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="171"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="181"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="201"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="223"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="251"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="262"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8031264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="354" mass="41861" checksum="230B6F41F96B8C11" modified="2023-09-13" version="1">MNPLTNIKNINKLNETEAELGVSSKVSWHQQYKDSAYIFIGGLPYKLTEGDILAVFSQYGEIVNINLVRDKKTGKSQGYCFIAYEDQRSTILAVDNFNGIQLLGRTIRVDHVANYRAPKDHEDDDEVTKKLRLEGCAPKIEPDSPPPAEDDGPPPKKLKKEKRSKKDRDEKKKKKKKEKKKSGGRDDHSSENSSEADSGDGSERQKMSEKPHQRSQNKTSKGSSDRTYERENHSDHRTDSLEKDRKDRNVSSKYSSDSSSNYRKEERREDLKRYREDDRRVEQSKDSNRQREHSHKERWREDKVREYRYKDREERTDRHKDRGRYNRDSDGEREERRRRKEDSRDKYDRCDGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BRS4</accession>
    <name>A0A9Q1BRS4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Alcohol dehydrogenase [NADP(+)] A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_25271</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldo/keto reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BRS4"/>
    <dbReference type="OrthoDB" id="416253at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000006">
        <property type="entry name" value="Aldo-keto reductase family 1 member A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11732">
        <property type="entry name" value="ALDO/KETO REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00798">
        <property type="entry name" value="ALDOKETO_REDUCTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00063">
        <property type="entry name" value="ALDOKETO_REDUCTASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="4">
        <location>
            <begin position="93"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8031908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="378" mass="42686" checksum="3B1099CCAC722822" modified="2023-09-13" version="1">MYEGIKKAKSGDVITDRSKEMERWVEHYSELFTTENTVSEEAINNISALPALDELDTESTIAELEKTINSLIDVMASRNKYATFSNGIKVPLLGLGTWKSKPGEVKNAIKTAIDAGYRHFDGAFSYENEKEVGNAYHEKITDGTVKREDLFITTKLWATYHRPERVEYGLRLSLKALQLDYVDLYLIHTPMGIQYKGDDVLFPNGENGEFLADDICPTETWKAMEKLVEKGLTKSIGVSNFTIRQMEDILKIAKVPIATNQVENHPCLDQKEMTDFCKSKNILITSYSPFGSPDRPWASDKDPNLLEDPVVKEIAGGKGCTPGQLLIAYHLCQGFLCIPKSVTPSRIQQNFQLEKPRPLSLLDVRRRQIYGLPRTISR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BT54</accession>
    <name>A0A9Q1BT54_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Proline-rich protein 5-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_25919</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PROTOR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BT54"/>
    <dbReference type="OrthoDB" id="2290221at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031932">
        <property type="term" value="C:TORC2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038203">
        <property type="term" value="P:TORC2 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013745">
        <property type="entry name" value="Bit61/PRR5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32428">
        <property type="entry name" value="TARGET OF RAPAMYCIN COMPLEX 2 SUBUNIT BIT61-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32428:SF2">
        <property type="entry name" value="TARGET OF RAPAMYCIN COMPLEX 2 SUBUNIT BIT61-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08539">
        <property type="entry name" value="HbrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8032402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="312" mass="34531" checksum="4E1E12D085DAD5D5" modified="2023-09-13" version="1">MSHLSVDTNHAAANGNSLLFGAASSSLADIEVTAVSPPFSTSKSFRRKRSSIVSLAVSGGRLLSHRRGSAMPVLQHGEFDVSTKSQQVLGLRNDEWNRVQTKVMTIFEGGKLGPSELFELNEMVRIAIKSEIGSFISEYYKKSLLKRGMEALYNAVEDDGDKQLLGSLSKVWNRFYSETLPTLRAIFYPIQTKGLTMSQITLLGFRDHVLLRTRLKEALLLNAHADIPSNISQMLLVLQNIREQTHPSEDYLQLESLVALVVCPYLGTRGLYMGGEVPVIKANPPPVEETVHRHPPLSKLHNSPVIPEVTIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BU12</accession>
    <name>A0A9Q1BU12_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_26126</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BU12"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8032569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="257" mass="29813" checksum="BB5DB441186FCAA8" modified="2023-09-13" version="1">MDPARDFVFWVFSFLFFDDDDDDDDDDGGGGGDGDGNDDDDDDDDDDDDDDHDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDGDHAHRYQLTSNAYHTKFRRETKQHSETFIDFVARLEDYAFYWLKPSEELRKNSEYSAIQNKIVSDQFLSTIRDETLRLKLVEKDDMRARDLAKLADDFVMQRRIIREEKKDEKSSIQSDADKSFAAKLAPRWEGPYTLEGKLDNVNFKVRTPKGLETKHVASL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BUZ6</accession>
    <name>A0A9Q1BUZ6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_23428</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BUZ6"/>
    <dbReference type="OrthoDB" id="10043391at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051836">
        <property type="entry name" value="Kremen_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002889">
        <property type="entry name" value="WSC_carb-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24269">
        <property type="entry name" value="KREMEN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24269:SF16">
        <property type="entry name" value="PROTEIN SLG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01822">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00321">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51212">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0768">Sushi</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040130601" evidence="10">
        <location>
            <begin position="17"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="WSC" evidence="12">
        <location>
            <begin position="25"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="11">
        <location>
            <begin position="123"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="182"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="246"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="191"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="214"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="247"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="257"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8033250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="315" mass="35519" checksum="3EA97C995328D2AC" modified="2023-09-13" version="1">MAVTFRLLSFIIITLSFELNLCVDAYSYIGCFQDATTDEERALPDSGQYLPRITIGSCRNFCAGQEFVYFGVEYDFCFCGAWDTDYSKHGELDEKSCNIPCRGNPSQMCGDRHALRVFVDNIPECQDIALENGQMHRLGLTATFSCIKGYNLQGNTQINCVYDDFRESFSWNGPPPICIEDVPTNTPRWMTTTKKATPTTYRTTQTTTKHRTTHTPTTFQSRSPQQISISPASTKDITTIKLREKVTTQPVHQQTTDSKKERETQAKDKEIVRKPTTQIKQDIKEIGNGTETQSSSHYSLILIVISLLGLIMGVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BVU6</accession>
    <name>A0A9Q1BVU6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_24009</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BVU6"/>
    <dbReference type="OrthoDB" id="5957838at2759"/>
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        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8033682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="84" mass="9455" checksum="B84248EA4D1DBD85" modified="2023-09-13" version="1">MPVKLDQGRRWLRVKQTLEAEHNITVNFSSTHVNYYTAYKYVVKEDDNALYSPGHPDLGDCSPKSTAASQKRTGNCGIDLNPKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BW58</accession>
    <name>A0A9Q1BW58_HOLLE</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="1" type="ORF">HOLleu_23963</name>
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    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="414730at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8033651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="219" mass="25370" checksum="7AA5AE50E012D0BE" modified="2023-09-13" version="1">MNKTKLLVFGTKVPNTENIILTLNKTRVPASRHTKFIGTIIDCKLSWKEHISSVCSKISRRNGVLNNPKYFLPQSTLLLLYSSLIFPHVNYNLLLWGNAKKRLLTVHSFAAKRAVLDICNVSFTHHTGPLFKSLSILSVFDLYKYHLGVFMHNFIHNKLPKCYSNFYVQNRHYHDCDTRKKHLLTIPYSRTCLTKSRIRHSGAVLWTSLHDHVKHCISL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BXN0</accession>
    <name>A0A9Q1BXN0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_21339</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BXN0"/>
    <dbReference type="OrthoDB" id="10067251at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8034479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="337" mass="38589" checksum="787571DD839F4EB9" modified="2023-09-13" version="1">MFGPHLDESCLRDRRLHENNLSKLARTRSDIVFLTQCKRLDLVPKGMRIRNPLQNCNRLLNHTAEALCHRTSCQLRSIALKSSYSRQNLLEQKLTKSTLDLQSKLPNTVDSQAVFKATENSYRWELSNLFRRKTGKVLSLAREDPILKPLVDLRHPILTDKFPRSYKAQPTAISRNNVFNISRHALAPAEKSHLEKGLSYCPTRQLDPVSLGFDLHQFICRVRLREYFGESQGDFVYTPTVVKQRAEWTPPEGRNQCIDRFTKSAMAHFDAFIAANRSKPVKPNLRPQERKAIGSLRRNPDITIRPADKGGDLTILNTADYIKEAESQLSNQVFIGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BXU7</accession>
    <name>A0A9Q1BXU7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Nuclear factor related to kappa-B-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_21568</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="70874at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002020">
        <property type="term" value="F:protease binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21865">
        <property type="entry name" value="DEUBAD_NFRKB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2430">
        <property type="entry name" value="NFRKB winged helix-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044867">
        <property type="entry name" value="DEUBAD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024867">
        <property type="entry name" value="NFRKB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025220">
        <property type="entry name" value="NFRKB_WH_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057748">
        <property type="entry name" value="NFRKB_WH_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038106">
        <property type="entry name" value="NFRKB_winged_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13052">
        <property type="entry name" value="NFRKB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13052:SF3">
        <property type="entry name" value="NUCLEAR FACTOR RELATED TO KAPPA-B-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14465">
        <property type="entry name" value="WHD_1st_NFRKB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25793">
        <property type="entry name" value="WHD_2nd_NFRKB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51916">
        <property type="entry name" value="DEUBAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEUBAD" evidence="4">
        <location>
            <begin position="82"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="247"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="364"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="800"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="889"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="946"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1016"/>
            <end position="1084"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1159"/>
            <end position="1184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1350"/>
            <end position="1382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1501"/>
            <end position="1586"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1618"/>
            <end position="1747"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1774"/>
            <end position="1795"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="49"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="249"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="288"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="301"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="389"/>
            <end position="404"/>
        </location>
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        </location>
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    </feature>
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        </location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
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            <begin position="1529"/>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1562"/>
            <end position="1578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1659"/>
            <end position="1674"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1710"/>
            <end position="1729"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1738"/>
            <end position="1747"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8034643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1795" mass="195127" checksum="5BB14F693C00DE26" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BY30</accession>
    <name>A0A9Q1BY30_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Serine-threonine/tyrosine-protein kinase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_21693</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BY30"/>
    <dbReference type="OrthoDB" id="5979581at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine-threonine/tyrosine-protein kinase catalytic" evidence="1">
        <location>
            <begin position="81"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8034730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="238" mass="27773" checksum="78BEC1449E947DB8" modified="2023-09-13" version="1">MEVGEDTAYVRLKTRFSSNGRLERWVGDLTGITSNRTTCMVNACKEDTSEGECEEFRKLAMVLKDMRHHMNIVEVIAVEVEKGKLAHPGIRCQKVLLTESLICKIYDFCTYPVTTDIINKMIQKNNSVMSWLAIETRANRTHTFKSDSFSSAVFVWEVFSWGEFANICNDLTERESMLSDKLNEWNYFSHTVVVRVVNYSYFYEKSWTYGDILKQGRSWDEIFRWPTYQMWGGGVSRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BYL0</accession>
    <name>A0A9Q1BYL0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">HOLleu_21936</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">Nanhai2018</strain>
            <tissue evidence="19">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BYL0"/>
    <dbReference type="OrthoDB" id="10045473at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050321">
        <property type="term" value="F:tau-protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14338">
        <property type="entry name" value="UBA_SIK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000003">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000156">
        <property type="entry name" value="Serine/threonine-protein kinase SIK3 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.80">
        <property type="entry name" value="Kinase associated domain 1, KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028375">
        <property type="entry name" value="KA1/Ssp2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001772">
        <property type="entry name" value="KA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057380">
        <property type="entry name" value="UBA_SIK1/2/3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF42">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE SIK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02149">
        <property type="entry name" value="KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23312">
        <property type="entry name" value="UBA_SIK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103243">
        <property type="entry name" value="KA1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50032">
        <property type="entry name" value="KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 19" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="30"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="17">
        <location>
            <begin position="311"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="KA1" evidence="18">
        <location>
            <begin position="1209"/>
            <end position="1258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="377"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="582"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="639"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="728"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="789"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="878"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1002"/>
            <end position="1025"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="420"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="591"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="730"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="752"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="828"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="843"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1002"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAJ8034909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1258" mass="138994" checksum="9F76E5FE3D0CAC81" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C071</accession>
    <name>A0A9Q1C071_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">WSC domain-containing protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_19545</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WSCD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8035769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C071"/>
    <dbReference type="OrthoDB" id="5985073at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051589">
        <property type="entry name" value="Sialate-O-sulfotransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45964:SF9">
        <property type="entry name" value="SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45964">
        <property type="entry name" value="WSCD FAMILY MEMBER CG9164"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13469">
        <property type="entry name" value="Sulfotransfer_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8035769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="358" mass="40713" checksum="9C831623309CFC06" modified="2023-09-13" version="1">MKCVTKRLICIIFFAMTLLTAIWAGWTTFQIIPSGRYVFLMKDLKPEPSVSKNHKKFIGHNFTTLAEGVANFSNGPTNLSVVNVVKSANLTWETNPTSHRLKDDAVNSARIQEAKKCLYLMKNIKPTPGPSPLTALASFPRSGNAYTRLLIQIATQFETSSVYWKTERFKPIKKDFPGVSQSFFKARGICTKTHKHDDKHMAFFSGGAILIVRDPHKSIISHFVRMFRKSGGTKKALELIQANDTQWIEISSKLAAEWRDLYKDWLNTASRVLVIPYERLCENTIEELEKIVTFLHQPIKVDQLQCAVSLYPCQCRDRTEFQPHYPEELPGYISEINNTLLTQFSTSLPSYGTKCPVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C0E0</accession>
    <name>A0A9Q1C0E0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Deleted in autism protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_19808</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DIPK family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8035963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C0E0"/>
    <dbReference type="OrthoDB" id="10035316at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020519">
        <property type="entry name" value="DIPK2A/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022049">
        <property type="entry name" value="FAM69_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32073:SF10">
        <property type="entry name" value="FAM69 PROTEIN-KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32073">
        <property type="entry name" value="GH11358P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12260">
        <property type="entry name" value="PIP49_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="FAM69 protein-kinase" evidence="5">
        <location>
            <begin position="358"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF12260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8035963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="596" mass="67548" checksum="9268A82418AB51C1" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C0I6</accession>
    <name>A0A9Q1C0I6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_17524</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8036873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C0I6"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040461067" evidence="1">
        <location>
            <begin position="20"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8036873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="137" mass="15656" checksum="0E980DC6055659A6" modified="2023-09-13" version="1">MKSIFAFGLLVFCWNVSIAQIIPMPIEIVPRKPVPDHGYCYRNMWSTCTTAGIKVQSKGEDCVECICDGEQQGALCCMKSPYPVVRFLGFCDVVLDTTTCQYERTPKFKHARHACCIRGYETSVIQEDLQNEFRDRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C0W9</accession>
    <name>A0A9Q1C0W9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">guanylate cyclase</fullName>
            <ecNumber evidence="3">4.6.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HOLleu_20259</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Nanhai2018</strain>
            <tissue evidence="17">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>GTP = 3',5'-cyclic GMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13665"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57746"/>
            <dbReference type="EC" id="4.6.1.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.2" evidence="3"/>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C0W9"/>
    <dbReference type="OrthoDB" id="1890790at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
        <property type="term" value="F:adenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004383">
        <property type="term" value="F:guanylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016941">
        <property type="term" value="F:natriuretic peptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017046">
        <property type="term" value="F:peptide hormone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007168">
        <property type="term" value="P:receptor guanylyl cyclase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001170">
        <property type="entry name" value="ANPR/GUC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050401">
        <property type="entry name" value="Cyclic_nucleotide_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11920:SF494">
        <property type="entry name" value="ATRIAL NATRIURETIC PEPTIDE RECEPTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11920">
        <property type="entry name" value="GUANYLYL CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00255">
        <property type="entry name" value="NATPEPTIDER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="13" id="KW-0141">cGMP biosynthesis</keyword>
    <keyword evidence="14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="12" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10 17" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="138"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="16">
        <location>
            <begin position="462"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="399"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ8036322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="574" mass="66391" checksum="39904F028AE3FC63" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C1H2</accession>
    <name>A0A9Q1C1H2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_17576</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8036911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C1H2"/>
    <dbReference type="OrthoDB" id="10434230at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="158"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="170"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8036911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="228" mass="24478" checksum="40304920B51D75FE" modified="2023-09-13" version="1">MSGKRGGGGEDDDDANFVVRDYGCWRPRSSPRPIRAATSDQGSHRIRSASPEVEQAIEALSPSAYQTDTTKLNQALQAGPPPPPPPPEALPPSLTPCKQPQTSLLHHPGNDPRQDSPKLLPPASQERVRSLTGTSSDLDSVGGSQWPLFSESKGPPQVNMNPALASSSPKDMDLEMGKSAKESLRKLSHSHSKDSMMTDPDAIELDTLSLTEGASQPEDEELPTFHDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C296</accession>
    <name>A0A9Q1C296_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Major facilitator superfamily domain-containing protein 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_17860</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. MFSD6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C296"/>
    <dbReference type="OrthoDB" id="515887at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024989">
        <property type="entry name" value="MFS_assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051717">
        <property type="entry name" value="MFS_MFSD6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16172:SF42">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16172">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 6-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12832">
        <property type="entry name" value="MFS_1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="61"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="88"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="121"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="257"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="298"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="335"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="375"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="414"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="445"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="470"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="380"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8037120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="522" mass="58054" checksum="7B939CAE9333B38D" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C4A0</accession>
    <name>A0A9Q1C4A0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Sodium-independent sulfate anion transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_18710</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C4A0"/>
    <dbReference type="OrthoDB" id="288203at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07042">
        <property type="entry name" value="STAS_SulP_like_sulfate_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="entry name" value="STAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011547">
        <property type="entry name" value="SLC26A/SulP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001902">
        <property type="entry name" value="SLC26A/SulP_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11814">
        <property type="entry name" value="SULFATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01740">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00916">
        <property type="entry name" value="Sulfate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SpoIIaa-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="92"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="115"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="143"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="169"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="193"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="219"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="265"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="319"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="394"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="418"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="456"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="domain" description="STAS" evidence="7">
        <location>
            <begin position="515"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8037804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="620" mass="68079" checksum="C9836FB7B8248102" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C682</accession>
    <name>A0A9Q1C682_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_16811</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C682"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="48"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="133"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8039175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="218" mass="23579" checksum="B4E3F8DBEB7372F7" modified="2023-09-13" version="1">MLHPMKEQDSGEDSVGTNDNCNTSTQEEEDFEIGLCYELYRSGKGDKTSTVTTTSDTPDNDTPLDSETSLREEDPDPTLPGPDCDPTTEIQDDNGRRGYGKAGEHTESLPTMSDKGSSDSGQDPPLTLRRSTRSTAGYNPNPHNELRGTLKAITGSYSLYESSSSPLALEGNTACICNMGVMDTVFILAAVGPTLAPSSQFSMRTSNDKFLHQFFLSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C7R7</accession>
    <name>A0A9Q1C7R7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">IgGFc-binding protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_13879</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8039773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C7R7"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035234">
        <property type="entry name" value="IgGFc-bd_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46534:SF1">
        <property type="entry name" value="IGGFC-BINDING PROTEIN N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46534">
        <property type="entry name" value="IGGFC_BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17517">
        <property type="entry name" value="IgGFc_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040174743" description="IgGFc-binding protein N-terminal domain-containing protein" evidence="1">
        <location>
            <begin position="17"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="domain" description="IgGFc-binding protein N-terminal" evidence="2">
        <location>
            <begin position="241"/>
            <end position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8039773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="546" mass="60917" checksum="E38B3AA5087B949D" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C883</accession>
    <name>A0A9Q1C883_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Serine/threonine-protein kinase ste20</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_13821</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8039726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C883"/>
    <dbReference type="OrthoDB" id="5979581at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="1"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8039726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="227" mass="25734" checksum="8DEDDBA00B8E1DA5" modified="2023-09-13" version="1">MFYQKDMCMIYNMKLGRLYNRWMGTVNVPNESSKCVVMSTVTADVLRTKEVHWDVFVKRVLELPKTNTLVNVEGIGVDKACLYIIHEHLTCETLFVRLNVHQNMGVPNTAFIPMFGSEVIAHMLEILEGMELLHSFGFVHPGLSTKKVLVTNQGNCKLYDFCLCEDACNVVRMKSQIVQTPRTGNHFAPEVILRNEYTKASDLWSTAVVLWELMSGNGIMAQSPIIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C8L3</accession>
    <name>A0A9Q1C8L3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transmembrane protein 45B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_12059</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM45 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C8L3"/>
    <dbReference type="OrthoDB" id="9987836at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006904">
        <property type="entry name" value="DUF716"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042127">
        <property type="entry name" value="TMEM45"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16007">
        <property type="entry name" value="EPIDIDYMAL MEMBRANE PROTEIN E9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16007:SF15">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 45B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04819">
        <property type="entry name" value="DUF716"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="55"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="94"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="119"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="152"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="212"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8041283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="296" mass="33935" checksum="D535DC017766E98B" modified="2023-09-13" version="1">MAVYWQDFAILGSFLFLLALIWTFQVSFQKFSYLMTTNQSSSNDVPHQNCFQTKWLEGIMACLCAAVVLICYFSNTYNKDNFESSEEENRSFVVVMWLPMFMYGAVAISSEALSPTYKALVKPFLIAAFLCEFSIVTTHIQSMAPGTDKTMYTPIYLLIYFSSLVVLAELVIPDMEDLLVLRCGTTLMRGTWYTQVGFTLCMTGREWLVEESVGITVLFVFLWHIILSWIVIRVMQNTIKAVLMDQPWVDEDYEDLRDDSSSKGKRRLVGQKEPVQLTREQLLATPWSSGYLKLAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C902</accession>
    <name>A0A9Q1C902_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Thioredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_14892</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the thioredoxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C902"/>
    <dbReference type="OrthoDB" id="2121326at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02947">
        <property type="entry name" value="TRX_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005746">
        <property type="entry name" value="Thioredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46115">
        <property type="entry name" value="THIOREDOXIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000077">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00421">
        <property type="entry name" value="THIOREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="5">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="33"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="36"/>
        </location>
    </feature>
    <feature type="site" description="Deprotonates C-terminal active site Cys" evidence="3">
        <location>
            <position position="27"/>
        </location>
    </feature>
    <feature type="site" description="Contributes to redox potential value" evidence="3">
        <location>
            <position position="34"/>
        </location>
    </feature>
    <feature type="site" description="Contributes to redox potential value" evidence="3">
        <location>
            <position position="35"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="4">
        <location>
            <begin position="33"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR000077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000077-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000077-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8040567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="113" mass="12651" checksum="365C704D6641B864" modified="2023-09-13" version="1">MPVQEIADHSSFMKLLEEAGDKLVLVDFYACWCGPCKQIANPLKKLSDDPKYASVIFAKVDVDVNGETAEACEINCMPTFQFFHQAKKVEEFSGANKAKLIELLNSLVEKYSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C903</accession>
    <name>A0A9Q1C903_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_15352</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C903"/>
    <dbReference type="OrthoDB" id="10463038at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="106"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8040907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="199" mass="21949" checksum="F04F42222B971513" modified="2023-09-13" version="1">MTLLCINHFQSEANGQYQCYLSDPDKASSVTSFRTVDTRSGGDSNNPDPPATEYQSSNKFPHHKVTLDDNGNDEGFGVFGCEATRNGKRKTRISTTRMRSDADFAPANELFTQTVNIEDEDVSITMYSPTGRDTNGIRWRKDNSSVISSQSGSAAYVIPGPIQLSDAGTYECQYNGERDMAKQGLNLLLVRGNLHFCLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CAT4</accession>
    <name>A0A9Q1CAT4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_12395</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CAT4"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="68"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8041545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="156" mass="16631" checksum="620557E14A4E06AC" modified="2023-09-13" version="1">MRLVSVKMDIKVSLAVLTAVATLLLILEKSNVSGKKMVVLNKPFFSSNVPKSGSFKNIKVVEATETISSDTNSKAASKTTKKGTETFTCTSQKDDNTTKGQLTPITEETPTTNSPEASTSYSMDNTRAVSTSVCNASSPTGAEKTATSERFTLLLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CBY1</accession>
    <name>A0A9Q1CBY1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Zinc finger protein 14</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_12796</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CBY1"/>
    <dbReference type="OrthoDB" id="3444203at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032502">
        <property type="term" value="P:developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001753">
        <property type="entry name" value="Si:ch211-119o8.6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000358">
        <property type="entry name" value="zinc finger protein 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000145">
        <property type="entry name" value="Zinc finger protein 574"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000202">
        <property type="entry name" value="Zinc finger protein 574"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000624">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000110">
        <property type="entry name" value="Zinc finger protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394:SF29">
        <property type="entry name" value="MYONEURIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="9"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="37"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="65"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="93"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="121"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="149"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="177"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="205"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="233"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="261"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="289"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8041874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="339" mass="40579" checksum="875941D8773E2341" modified="2023-09-13" version="1">MAHTGERPFKCQFCEKSFIVAGHCKRHEMTHTGDKPFKCQYCEKLFSKKRSCKLHEMASTSERPFKCQFCKKSFKFKGNCKRHEMTHTGERPFKCQFCEKSFQTKWDCKIHVMTHTGDRPFKCGYCEKSFLKKRDWTIHEMIHTDERTFKCQYCEKSFVMKGNCKRHEMTHTGEKPFKCQFCEKSFQTKWDRKIHEMIHTGDKPFKCQYCDKSFIIKRSCKLHEMTHTGERPFKCQFCEKFFILKGNCKRHELTHIGDKPFKCLFCEKSFQTKWDCKMHEMTHTVDRPFKCQFCEKSFKKKGNCKIHEMIHTGEQGKDSPNVSIVRSHIKQKGTVKDRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CC00</accession>
    <name>A0A9Q1CC00_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_13592</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8042521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CC00"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8042521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="64" mass="7242" checksum="1A862766B6FAB4BD" modified="2023-09-13" version="1">MAAPMANFSPDREKSRFPGMGGFPELLELDVACVVFYSGGRIVRVKSKETKCRTRYTVVRKQTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CC66</accession>
    <name>A0A9Q1CC66_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">RING-type E3 ubiquitin-protein ligase PPIL2</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">CYC4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Probable inactive peptidyl-prolyl cis-trans isomerase-like 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">HOLleu_09862</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="24">Nanhai2018</strain>
            <tissue evidence="24">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Has a ubiquitin-protein ligase activity acting as an E3 ubiquitin protein ligase or as an ubiquitin-ubiquitin ligase promoting elongation of ubiquitin chains on substrates. By mediating 'Lys-48'-linked polyubiquitination of proteins could target them for proteasomal degradation. May also function as a chaperone, playing a role in transport to the cell membrane of BSG/Basigin for instance. Probable inactive PPIase with no peptidyl-prolyl cis-trans isomerase activity. As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Component of the minor spliceosome, which splices U12-type introns. Within this complex, interacts with PRPF8/PRP8, EFTUD2/SNU114 and PLRG1. Interacts with isoform 2 of BSG. Interacts (via the PPIase cyclophilin-type domain) with CRNKL1; they may form a trimeric complex with HSP90.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cyclophilin-type PPIase family. PPIL2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5"/>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8042963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CC66"/>
    <dbReference type="OrthoDB" id="30774at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01923">
        <property type="entry name" value="cyclophilin_RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16663">
        <property type="entry name" value="RING-Ubox_PPIL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000079">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000018">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase-like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044666">
        <property type="entry name" value="Cyclophilin_A-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026951">
        <property type="entry name" value="PPIL2_U-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625:SF1">
        <property type="entry name" value="RING-TYPE E3 UBIQUITIN-PROTEIN LIGASE PPIL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04641">
        <property type="entry name" value="Rtf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00504">
        <property type="entry name" value="Ubox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51698">
        <property type="entry name" value="U_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0007">Acetylation</keyword>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="7" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="14" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="U-box" evidence="23">
        <location>
            <begin position="36"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="22">
        <location>
            <begin position="285"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="443"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="443"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="458"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00059251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00061807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00073734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00078275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00079124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="KAJ8042963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="535" mass="60210" checksum="3597611D8C1FB723" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CD55</accession>
    <name>A0A9Q1CD55_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_13607</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8042533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CD55"/>
    <dbReference type="OrthoDB" id="10071239at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8042533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="137" mass="15891" checksum="1145A6F0737BE7A1" modified="2023-09-13" version="1">MYICRHQTSNRQSHNEDCPLKTKTGDIITDRNKQICRWVKHYLEIYSRKNIVIDEALTAIKDLPVVEELDYEPTLEELIKAIDTPKSNQVAGKDGIPPEIIKFGKLVILKHLHNLLCLCWKEDKVPQDMRNAKIVTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CDC7</accession>
    <name>A0A9Q1CDC7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein ANTAGONIST OF LIKE HETEROCHROMATIN PROTEIN 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_10571</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CDC7"/>
    <dbReference type="OrthoDB" id="2668416at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027806">
        <property type="entry name" value="HARBI1_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13359">
        <property type="entry name" value="DDE_Tnp_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE Tnp4" evidence="3">
        <location>
            <begin position="74"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8043473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="214" mass="24587" checksum="9A705F3919CFAE3D" modified="2023-09-13" version="1">MASGDSYKSLEYNFRVANNTIRRGGGGIVPETCEALYEEFKDEYFKVPNNPDEWREVARGFQERWNFPKCLGAIDGKHVAKKAPGRTVSLFYNYKGFFSVVMMALVDSKYRFLNVDVGSHGSCSDGGLFMHTELKECLENDTLGVPPPEPYPNDDRPMPYCIVGDDAFPRRRWLMKPYPQRLMTKEERISIIVCPGHDALCKMRLEFWHIGRPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CEH4</accession>
    <name>A0A9Q1CEH4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_10236</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CEH4"/>
    <dbReference type="OrthoDB" id="6414146at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8043238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="121" mass="13698" checksum="A9DDB9120DEFD134" modified="2023-09-13" version="1">MLVKIKNTMTDRAAVNKAFVRKFEAWRADEYAQCALYEWERVENDGKLGREKHIVWNRGNESASMLAVRSICNCLGPDADPQAGFSFNVHLEDRYQEKSSVRQSAVVAVFVASSNSRNNTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CEM3</accession>
    <name>A0A9Q1CEM3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phytoene dehydrogenase, chloroplastic/chromoplastic</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_10665</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CEM3"/>
    <dbReference type="OrthoDB" id="5046242at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050464">
        <property type="entry name" value="Zeta_carotene_desat/Oxidored"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42923:SF43">
        <property type="entry name" value="AMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42923">
        <property type="entry name" value="PROTOPORPHYRINOGEN OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040185472" evidence="1">
        <location>
            <begin position="22"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="domain" description="Amine oxidase" evidence="2">
        <location>
            <begin position="54"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8043540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="533" mass="60632" checksum="CE360B718D1ED0A2" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CFL3</accession>
    <name>A0A9Q1CFL3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">RNA transcription, translation and transport factor protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_07042</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CFL3"/>
    <dbReference type="OrthoDB" id="514167at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019265">
        <property type="entry name" value="RTRAF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15924">
        <property type="entry name" value="CLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10036">
        <property type="entry name" value="RLL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8044317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="237" mass="27472" checksum="A1A24B8EC14B89DB" modified="2023-09-13" version="1">MFKRKLRALKYHNLDVNIQDEEQWRNLIVWLEDQKIRLYKIEDRKNLKDICSGKWDKALNQYLDDLSCETDRSNRNSLCDWLLGTAVRQEYGENVEKFKSMQTEANTSDATDDPFAHLDVNSPDFKVGLLSLCQLLKIPQHYDPVTSLQAISILIKERLSEEAQDATLEEGEDFDLKGYEVGFETRDDVLNNASCILRLLHLQELRELQTRINEVIVSVQSITANPKTDQRLGKVGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CGQ5</accession>
    <name>A0A9Q1CGQ5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Rhodopsin, GQ-coupled</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_07436</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CGQ5"/>
    <dbReference type="OrthoDB" id="5963071at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00637">
        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45698:SF1">
        <property type="entry name" value="TRACE AMINE-ASSOCIATED RECEPTOR 13C-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45698">
        <property type="entry name" value="TRACE AMINE-ASSOCIATED RECEPTOR 19N-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0675">Receptor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="26"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="107"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="145"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="188"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="267"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="304"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="7">
        <location>
            <begin position="38"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8044646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="364" mass="42001" checksum="2C368835868A80F3" modified="2023-09-13" version="1">MESTELTATTQSSFENIQEGDVLMKVFYAIFAILGVMGNGLVLFVFYKTPDLHVPTNILIGHQSLVDLVFSVILFLNFIPPSIDLETLGVSHPVLSNLTCKVWVSEFVYWSILKVSTINLVFLTLERYFAVVHPIKFIKIMNRRIAISMCILAWILGIVLSIYFPFIHRVEDDGDCTSRSVMRGSPERYAIVVLSVVTTLILPVGVMLYAYLMIFWTLRPVKDLPDTELPSNATTIHMGDTQTNGVDSRKMEFTRKAGFRDRCRRNVLITFFLVSLSYTICWTPDMVLYFHHNVVTRHEWSDPIHQFVILLAVSNSWINPVIYTWKYRGFQKGLKDSLLKSRKDVTRVFRTCYTYVHNRNIQFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CHW3</accession>
    <name>A0A9Q1CHW3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_08625</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CHW3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040162682" evidence="2">
        <location>
            <begin position="22"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="164"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8045588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="190" mass="20746" checksum="230046DC36EBD34B" modified="2023-09-13" version="1">MALRVLAYLTVVLGMALCAEAYYLCLAGERLSCTPPEDTPDEENRCNNITDPGPLWIKTCAEHENACFNASHTYMEGGYSYENLTFGCIYADDKDDNCEVGKSILEMKHPVAALGFRLFKEELNERGVTISNEIFETCVCKDENCNGMLQAREIADNNNRASSVVWPVIIGAMVATVALIAIGAAVSLCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CK29</accession>
    <name>A0A9Q1CK29_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Tubulin-folding cofactor B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_09163</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TBCB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8046014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CK29"/>
    <dbReference type="OrthoDB" id="5295208at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035371">
        <property type="term" value="C:microtubule plus-end"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043014">
        <property type="term" value="F:alpha-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051010">
        <property type="term" value="F:microtubule plus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007023">
        <property type="term" value="P:post-chaperonin tubulin folding pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007021">
        <property type="term" value="P:tubulin complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01789">
        <property type="entry name" value="Ubl_TBCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.190:FF:000013">
        <property type="entry name" value="Tubulin-folding cofactor B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.190">
        <property type="entry name" value="CAP Gly-rich-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036859">
        <property type="entry name" value="CAP-Gly_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000938">
        <property type="entry name" value="CAP-Gly_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045172">
        <property type="entry name" value="TBCB_Ubl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18916">
        <property type="entry name" value="DYNACTIN 1-RELATED MICROTUBULE-BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18916:SF85">
        <property type="entry name" value="TUBULIN-FOLDING COFACTOR B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01302">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14560">
        <property type="entry name" value="Ubiquitin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01052">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74924">
        <property type="entry name" value="Cap-Gly domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00845">
        <property type="entry name" value="CAP_GLY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50245">
        <property type="entry name" value="CAP_GLY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CAP-Gly" evidence="5">
        <location>
            <begin position="188"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8046014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="250" mass="28132" checksum="D42A25430C315EC5" modified="2023-09-13" version="1">MSGNIEVLTQSYVNVFITSNLTSFEAQKRFEKDITIAALKGKLELIAGANANSMQLQLLSKEGKLVGNLDNDDALLGSYPIDDNMKIHVVDNSKGKGEFEDVSQVEKYEISEEEYSKRSDSVRAFKMRNKLGNFKDVDPEEQAKREAERKAAEEKEAELIANIKEGCRCEVDNPSNPPKKRGTVMYVGKTDFKPGFWVGIQYDEPLGKNDGSVKGRRYFSCEPKYGGFVKPQHVTVGHFPVELEEEMDEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CNR7</accession>
    <name>A0A9Q1CNR7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Zinc finger MYM-type protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_06893</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CNR7"/>
    <dbReference type="OrthoDB" id="9950531at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025398">
        <property type="entry name" value="DUF4371"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006580">
        <property type="entry name" value="Znf_TTF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45749">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45749:SF21">
        <property type="entry name" value="DUF4371 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14291">
        <property type="entry name" value="DUF4371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00597">
        <property type="entry name" value="ZnF_TTF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TTF-type" evidence="2">
        <location>
            <begin position="47"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="306"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="310"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8047794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="485" mass="52882" checksum="EED0C7C0BB068CA8" modified="2023-09-13" version="1">MQTCTSYSVPNANISDQSQPSNDEPGVPPGPHQPSEFSFPARTYGKQNRAFQTHWFRSFPWLHYSETNDSVLCHTCVTADKQKKSFNARSSEKAFLVEGFCNWKKALEKFRDHQKSTCHRLAMHAESQRMYGDIGEKMDATFAEERKKNRMIFIKILENVKFLSRQGLAFQGNEESGNFLQLLKLRAADDPHLCDWMKKKRDKYTSHDIQNEIVRIMATDILCDIADSVSDAKFFSILADECTDASNKEQLTICLRWIDQALEPNEDFVGFYEIPNITANTIVGAIKGVLQRLTLSLNNCRGQCFDAGGPIPPPPPPPPTPPPPHPPPPPPPPPPTPPPPHPPPPPPPPPPTPPPPHPPPPPPPPPPTPPPPHPPPPPPPPPPTPPPPHPPPPPPPPPPTPPPPHPPPPPPPPPPTPPPPHPPPPPPPPPPTPPPPHPPPPPPPPPPTPPPPHPPPPPPPPPPTPPPPHPPPPPPPPQARGENLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CP89</accession>
    <name>A0A9Q1CP89_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Papilin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_00307</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CP89"/>
    <dbReference type="OrthoDB" id="5950222at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048019">
        <property type="term" value="F:receptor antagonist activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050431">
        <property type="term" value="F:transforming growth factor beta binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007179">
        <property type="term" value="P:transforming growth factor beta receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00109">
        <property type="entry name" value="Kunitz-type"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.410.10:FF:000005">
        <property type="entry name" value="Pancreatic trypsin inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.10">
        <property type="entry name" value="Pancreatic trypsin inhibitor Kunitz domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002223">
        <property type="entry name" value="Kunitz_BPTI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036880">
        <property type="entry name" value="Kunitz_BPTI_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020901">
        <property type="entry name" value="Prtase_inh_Kunz-CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45938">
        <property type="entry name" value="ACP24A4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45938:SF11">
        <property type="entry name" value="WAP, KAZAL, IMMUNOGLOBULIN, KUNITZ AND NTR DOMAIN-CONTAINING PROTEIN 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00014">
        <property type="entry name" value="Kunitz_BPTI"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00759">
        <property type="entry name" value="BASICPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00131">
        <property type="entry name" value="KU"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57362">
        <property type="entry name" value="BPTI-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00280">
        <property type="entry name" value="BPTI_KUNITZ_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50279">
        <property type="entry name" value="BPTI_KUNITZ_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="6">
        <location>
            <begin position="43"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="6">
        <location>
            <begin position="99"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="6">
        <location>
            <begin position="155"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="6">
        <location>
            <begin position="209"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8048129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="267" mass="29188" checksum="0E5F8CB8389EED43" modified="2023-09-13" version="1">MSGTQESLIMLLKVWSLNFLVFAVLQVTFCNHSEARFSIILDCQKPLETGMCRGYFRKWGFKDGKCQQFVYGGCGGNGNNFNTEDECNDACVSAPVSVCELPLETGPCRAARTRFGYKGGKCVKFTYGGCRGNENNFATEAECRSTCVSAPVSVCELPLETGPCRAARTRFGYKDGKCVKFTYGGCRGNENNFATEAECISACGSVSVCDQPLKTGPCRAIMPRFGYKNGQCVKFIYGGCRGNENNFKTRKQCQKVCGGAIYTKNGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CPE7</accession>
    <name>A0A9Q1CPE7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP-dependent DNA helicase</fullName>
            <ecNumber evidence="1">5.6.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_01031</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.3" evidence="1"/>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CPE7"/>
    <dbReference type="OrthoDB" id="10036850at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010285">
        <property type="entry name" value="DNA_helicase_pif1-like_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025476">
        <property type="entry name" value="Helitron_helicase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051055">
        <property type="entry name" value="PIF1_helicase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47642">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47642:SF5">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14214">
        <property type="entry name" value="Helitron_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05970">
        <property type="entry name" value="PIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0227">DNA damage</keyword>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="1" id="KW-0234">DNA repair</keyword>
    <keyword evidence="1" id="KW-0347">Helicase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helitron helicase-like" evidence="5">
        <location>
            <begin position="163"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="DNA helicase Pif1-like DEAD-box helicase" evidence="4">
        <location>
            <begin position="800"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="85"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="604"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="724"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="107"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF14214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8048640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="891" mass="102532" checksum="F04F0F8A4BAB2B32" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CQW1</accession>
    <name>A0A9Q1CQW1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_02565</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CQW1"/>
    <dbReference type="OrthoDB" id="416119at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19446">
        <property type="entry name" value="REVERSE TRANSCRIPTASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8049706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="125" mass="14486" checksum="029A4C087E7E654F" modified="2023-09-13" version="1">MSNNKSPGPDGYPMEFFKYFWNDLGDYIRRALNDCYKTGSLSIPLSRGVITLIPKPNKDRRYLKHWRPITLLPVIYKVASSCVANRMKRILSSLIASDQKRIPKGKVYWGKHKATVRPNAVYRIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CR99</accession>
    <name>A0A9Q1CR99_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NLR family CARD domain-containing protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_03181</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CR99"/>
    <dbReference type="OrthoDB" id="120976at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312">
        <property type="entry name" value="NACHT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312:SF2">
        <property type="entry name" value="NUCLEOTIDE-BINDING OLIGOMERIZATION DOMAIN-CONTAINING PROTEIN 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NACHT" evidence="1">
        <location>
            <begin position="199"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8050107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="786" mass="90572" checksum="13212A8ACB62EEBD" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CTA1</accession>
    <name>A0A9Q1CTA1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">exodeoxyribonuclease III</fullName>
            <ecNumber evidence="4">3.1.11.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_03128</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.</text>
            <dbReference type="EC" id="3.1.11.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DNA repair enzymes AP/ExoA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.11.2" evidence="4"/>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CTA1"/>
    <dbReference type="OrthoDB" id="8961218at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003906">
        <property type="term" value="F:DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008311">
        <property type="term" value="F:double-stranded DNA 3'-5' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09076">
        <property type="entry name" value="L1-EN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004808">
        <property type="entry name" value="AP_endonuc_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748">
        <property type="entry name" value="AP ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748:SF26">
        <property type="entry name" value="ENDONUCLEASE_EXONUCLEASE_PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="8">
        <location>
            <begin position="9"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8050074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="302" mass="33921" checksum="4C98B8014801D4A0" modified="2023-09-13" version="1">MAADPLSLLTLNVNGMRDSRKRSRIFQFCRSLGVDFVFLQETHIVNEDDVHTWSFEWGGGLHAPFGSSTSCGSVILVSPRWTPLVGKVDSDHEGRLVCITIKHPCGNLFLCNVYAPNRPSDRRDFFMTLPSFVPGSAPCILAGGFNCVPDTQLDRMTSCTSSGCSVGMTELDMFTKNHDMVDVWRVQHPGSSVLTWHRPDGTDASRLDRVYSPLALSPSDCEVVACSISDHDAVTVSFQPPDSWPARRGFWKMNTDILMEDDYCTQFKARYGGWRTLKPAFSTGLEWWDDIKSRIKQFTVEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H0I1</accession>
    <name>A0A9Q1H0I1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_27005</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H0I1"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8030552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="164" mass="18734" checksum="26FBB8AF6B149554" modified="2023-09-13" version="1">MSLKCSVIEVFDKRDVSPTTEGISTASSRILSSESVHVSEISTISSDEGINVITYVLVIVLVTCLIILMLVVCTVIVCSRMRRKERHTRREFSEDNSRQYDRAIFSVSNTNYTPDYMYVYEEESNRTKDSAPTYESKIHQQPSTDECTTYEETRHVYLGTDFSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H2A2</accession>
    <name>A0A9Q1H2A2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_26844</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H2A2"/>
    <dbReference type="OrthoDB" id="10680750at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040303567" evidence="2">
        <location>
            <begin position="27"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="157"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="205"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="219"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="247"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8030418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="295" mass="33392" checksum="CDA474EE92EB6697" modified="2023-09-13" version="1">MKKLLIPANITPVILVLMVSHSVLEANNSVEFYFFGSKDDARFLFAGDDVVLMCEAKGFLNPTLKLTFNGNELFDGDNLVAQNDSRLVNRNQKEQDTRQDTVNHLFGGSVQINHHLSQYVYATASERAEETTFAYDSVPYTTNQDDDYDDDKRHVRPPTVNTEIPQANPVYESAPSNEMPPHHLQDVALDKKVLSLDPVYKETPTKITHLKENNGNTEQVDKSKKSSYEPVDRSDQERPPSAVYATVDRKNKNKKSETNASNLEEKAEMSNTDPTRDHVYAYSNAEIEYDAITRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H2I5</accession>
    <name>A0A9Q1H2I5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Craniofacial development protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_29258</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H2I5"/>
    <dbReference type="OrthoDB" id="410381at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027124">
        <property type="entry name" value="Swc5/CFDP1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23227">
        <property type="entry name" value="BUCENTAUR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23227:SF67">
        <property type="entry name" value="CRANIOFACIAL DEVELOPMENT PROTEIN 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="1">
        <location>
            <begin position="6"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8029776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="312" mass="35988" checksum="4F184E442AF5144F" modified="2023-09-13" version="1">MTLKFGTWNVRTLLDSSRTDRPERRTALIARELKRYQVDIAALSETRFADKRQLTEIGGGYTFFWSGRPSTERRKARVDFAVILQIVNKLAKLPERVNDRLMTLQLPLGKKKNAIIISAYSPTMTNPDDVKDQFYEELDTIITRVPQADKLLGDFNARVGSDHRVWEKVIEKHGIVDRKIAEEFSRDLDAKLTEIPMALTNIEDQWASFRDTVYSVALEHLGPAKRKHQDWFDENSENIHNLLVKKRLMRIHQDDPSSVSQKAAFVNKRQVEQKKLRDMQDSRLSQMADEIQASPVLNADGSKLLTEKTQIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H5K1</accession>
    <name>A0A9Q1H5K1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">D-galactoside-specific lectin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_23975</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H5K1"/>
    <dbReference type="OrthoDB" id="5970528at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000922">
        <property type="entry name" value="Lectin_gal-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043159">
        <property type="entry name" value="Lectin_gal-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46780">
        <property type="entry name" value="PROTEIN EVA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02140">
        <property type="entry name" value="SUEL_Lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50228">
        <property type="entry name" value="SUEL_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="154"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="SUEL-type lectin" evidence="3">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8033658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="188" mass="20610" checksum="1195B3F8EFAEB537" modified="2023-09-13" version="1">MNVVCLNTTTINVVSATYGYNWNNQKAKLKCDDWKNKYIRDLDDMACRNPNSSDVVADKCNGLLQCTISATNKVLGDPCEGVTKYFELYYNCNQDGSDVDSGSSYENNTNSDEIQVDIDDGVEDNNDDKNDSDDDNDNGDYHNNDDEDGNCLSAVAVVAFAAGIPFGAVVPISIIVILACILAHRIKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H5R9</accession>
    <name>A0A9Q1H5R9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">N-acetylgalactosaminyltransferase 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_24237</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H5R9"/>
    <dbReference type="OrthoDB" id="6119243at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004653">
        <property type="term" value="F:polypeptide N-acetylgalactosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675">
        <property type="entry name" value="N-ACETYLGALACTOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675:SF68">
        <property type="entry name" value="N-ACETYLGALACTOSAMINYLTRANSFERASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="9">
        <location>
            <begin position="190"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="117"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="120"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8033868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="303" mass="34575" checksum="C47CA436897290AF" modified="2023-09-13" version="1">MSITISGRRHYCTIRRIVLASFAFFAASPLLVIFLLQEPGESFGLEKSLRNFRSNSTLDHKIPEGNIKVQPDLKDVQYSSKNNRVNRLHQPVNKAQQVVGHKNIELPVLKEGIIGNYEPRMPRKGPDGPGEGGKKVGRIPEEEEQYAQSYKEHGFNMVNSEKISLDRTVPDLRDSECKFWHYPSKLPLSSVVIVFCNEGWSTLMRTVHSVINETPPELLLEIVMVDDFSDKEHLQTKLEDYIKIPFFQNKVKLVRNVKREGLIRSRIIGAQHARAEVLIFLDAHCECSPNWLPPLVAEIAVNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H6R0</accession>
    <name>A0A9Q1H6R0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">H(+)-transporting two-sector ATPase</fullName>
            <ecNumber evidence="2">7.1.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_22395</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.2" evidence="2"/>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H6R0"/>
    <dbReference type="OrthoDB" id="10048076at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046034">
        <property type="term" value="P:ATP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031686">
        <property type="entry name" value="ATP-synth_a_Xtn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022878">
        <property type="entry name" value="V-ATPase_asu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43607:SF1">
        <property type="entry name" value="H(+)-TRANSPORTING TWO-SECTOR ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43607">
        <property type="entry name" value="V-TYPE PROTON ATPASE CATALYTIC SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16886">
        <property type="entry name" value="ATP-synt_ab_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-1278">Translocase</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ATPsynthase alpha/beta subunit barrel-sandwich" evidence="9">
        <location>
            <begin position="39"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="ATPase F1/V1/A1 complex alpha/beta subunit nucleotide-binding" evidence="8">
        <location>
            <begin position="108"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8035234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="192" mass="21403" checksum="06300C13501E9A62" modified="2023-09-13" version="1">MWIQRATACGLGYPFKTVGSQVHHRWWFLWVGARKFSNRMWLMLPPKSRGTVTWIAPAGQSFVEPKDVVLETEFDGEKTKHTMVQVWPVRQMRPTSDKLPANYPLLTGQRVLDALFPCVQGGTTAIPGAFGCGKTVISQSLSKYSNSDVIIYVGCGERGNEMSEVLRDFPEVGVLRMVAMKPCGQIPCSAKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H7E0</accession>
    <name>A0A9Q1H7E0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="33">Carnitine O-acetyltransferase</fullName>
            <ecNumber evidence="31">2.3.1.137</ecNumber>
            <ecNumber evidence="32">2.3.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="34">Carnitine acetyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="38" type="ORF">HOLleu_15834</name>
    </gene>
    <organism evidence="38 39">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="38" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="38">Nanhai2018</strain>
            <tissue evidence="38">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="30">Catalyzes the reversible transfer of acyl groups from carnitine to coenzyme A (CoA) and regulates the acyl-CoA/CoA ratio. Also plays a crucial role in the transport of fatty acids for beta-oxidation. Responsible for the synthesis of short- and branched-chain acylcarnitines. Active towards some branched-chain amino acid oxidation pathway (BCAAO) intermediates. Trans-2-enoyl-CoAs and 2-methylacyl-CoAs are poor substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>(R)-carnitine + acetyl-CoA = O-acetyl-(R)-carnitine + CoA</text>
            <dbReference type="Rhea" id="RHEA:21136"/>
            <dbReference type="ChEBI" id="CHEBI:16347"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57589"/>
            <dbReference type="EC" id="2.3.1.7"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:21137"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>2,6-dimethylheptanoyl-CoA + (R)-carnitine = O-2,6-dimethylheptanoyl-(R)-carnitine + CoA</text>
            <dbReference type="Rhea" id="RHEA:45004"/>
            <dbReference type="ChEBI" id="CHEBI:16347"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:84843"/>
            <dbReference type="ChEBI" id="CHEBI:84847"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:45005"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>2-methylbutanoyl-CoA + (R)-carnitine = O-2-methylbutanoyl-(R)-carnitine + CoA</text>
            <dbReference type="Rhea" id="RHEA:44992"/>
            <dbReference type="ChEBI" id="CHEBI:16347"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57336"/>
            <dbReference type="ChEBI" id="CHEBI:84840"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:44993"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>2-methylpropanoyl-CoA + (R)-carnitine = O-isobutanoyl-(R)-carnitine + CoA</text>
            <dbReference type="Rhea" id="RHEA:44988"/>
            <dbReference type="ChEBI" id="CHEBI:16347"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57338"/>
            <dbReference type="ChEBI" id="CHEBI:84838"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:44989"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>3-hydroxybutanoyl-CoA + (R)-carnitine = O-3-hydroxybutanoyl-(R)-carnitine + CoA</text>
            <dbReference type="Rhea" id="RHEA:45000"/>
            <dbReference type="ChEBI" id="CHEBI:16347"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:78611"/>
            <dbReference type="ChEBI" id="CHEBI:84842"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:45001"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>3-methylbutanoyl-CoA + (R)-carnitine = O-3-methylbutanoyl-(R)-carnitine + CoA</text>
            <dbReference type="Rhea" id="RHEA:44984"/>
            <dbReference type="ChEBI" id="CHEBI:16347"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57345"/>
            <dbReference type="ChEBI" id="CHEBI:70819"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:44985"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>4,8-dimethylnonanoyl-CoA + (R)-carnitine = O-4,8-dimethylnonanoyl-(R)-carnitine + CoA</text>
            <dbReference type="Rhea" id="RHEA:44860"/>
            <dbReference type="ChEBI" id="CHEBI:16347"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:77061"/>
            <dbReference type="ChEBI" id="CHEBI:84654"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:44861"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>acetoacetyl-CoA + (R)-carnitine = O-3-oxobutanoyl-(R)-carnitine + CoA</text>
            <dbReference type="Rhea" id="RHEA:44996"/>
            <dbReference type="ChEBI" id="CHEBI:16347"/>
            <dbReference type="ChEBI" id="CHEBI:57286"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:84841"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="28">
            <dbReference type="Rhea" id="RHEA:44997"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>butanoyl-CoA + (R)-carnitine = O-butanoyl-(R)-carnitine + CoA</text>
            <dbReference type="Rhea" id="RHEA:44980"/>
            <dbReference type="ChEBI" id="CHEBI:16347"/>
            <dbReference type="ChEBI" id="CHEBI:21949"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57371"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:44981"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>decanoyl-CoA + (R)-carnitine = O-decanoyl-(R)-carnitine + CoA</text>
            <dbReference type="Rhea" id="RHEA:44828"/>
            <dbReference type="ChEBI" id="CHEBI:16347"/>
            <dbReference type="ChEBI" id="CHEBI:28717"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:61430"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:44829"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>hexanoyl-CoA + (R)-carnitine = O-hexanoyl-(R)-carnitine + CoA</text>
            <dbReference type="Rhea" id="RHEA:44972"/>
            <dbReference type="ChEBI" id="CHEBI:16347"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:62620"/>
            <dbReference type="ChEBI" id="CHEBI:84834"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:44973"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>octanoyl-CoA + (R)-carnitine = O-octanoyl-(R)-carnitine + CoA</text>
            <dbReference type="Rhea" id="RHEA:17177"/>
            <dbReference type="ChEBI" id="CHEBI:16347"/>
            <dbReference type="ChEBI" id="CHEBI:18102"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57386"/>
            <dbReference type="EC" id="2.3.1.137"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:17178"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>propanoyl-CoA + (R)-carnitine = O-propanoyl-(R)-carnitine + CoA</text>
            <dbReference type="Rhea" id="RHEA:44976"/>
            <dbReference type="ChEBI" id="CHEBI:16347"/>
            <dbReference type="ChEBI" id="CHEBI:53210"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57392"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="29">
            <dbReference type="Rhea" id="RHEA:44977"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="5">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Mitochondrion inner membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Matrix side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 36">Belongs to the carnitine/choline acetyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="38">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.137" evidence="31"/>
    <dbReference type="EC" id="2.3.1.7" evidence="32"/>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H7E0"/>
    <dbReference type="OrthoDB" id="240216at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004092">
        <property type="term" value="F:carnitine O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008458">
        <property type="term" value="F:carnitine O-octanoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019254">
        <property type="term" value="P:carnitine metabolic process, CoA-linked"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.10:FF:000001">
        <property type="entry name" value="Carnitine O-acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.70:FF:000002">
        <property type="entry name" value="Carnitine O-acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.70">
        <property type="entry name" value="Choline/Carnitine o-acyltransferase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000542">
        <property type="entry name" value="Carn_acyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039551">
        <property type="entry name" value="Cho/carn_acyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042231">
        <property type="entry name" value="Cho/carn_acyl_trans_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22589:SF103">
        <property type="entry name" value="CARNITINE O-ACETYL-TRANSFERASE, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22589">
        <property type="entry name" value="CARNITINE O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00755">
        <property type="entry name" value="Carn_acyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00440">
        <property type="entry name" value="ACYLTRANSF_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="16 36" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="15" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="39" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 36" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Choline/carnitine acyltransferase" evidence="37">
        <location>
            <begin position="68"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="35">
        <location>
            <position position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00050133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00050207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00050851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00050860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00051087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00051518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00051534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00051554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00051955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00051962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00052310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00052568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00053012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00058613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00066418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00066910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="ARBA" id="ARBA00074976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="ARBA" id="ARBA00079830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="35">
        <source>
            <dbReference type="PIRSR" id="PIRSR600542-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="36">
        <source>
            <dbReference type="RuleBase" id="RU003801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="37">
        <source>
            <dbReference type="Pfam" id="PF00755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="38">
        <source>
            <dbReference type="EMBL" id="KAJ8038412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="39">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="657" mass="74085" checksum="EF2884225B303885" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H964</accession>
    <name>A0A9Q1H964_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Lipopolysaccharide-induced tumor necrosis factor-alpha factor-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_17581</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endosome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Late endosome membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Lysosome membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CDIP1/LITAF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8036916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H964"/>
    <dbReference type="OrthoDB" id="4713066at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006629">
        <property type="entry name" value="LITAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037519">
        <property type="entry name" value="LITAF_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23292:SF6">
        <property type="entry name" value="FI16602P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23292">
        <property type="entry name" value="LIPOPOLYSACCHARIDE-INDUCED TUMOR NECROSIS FACTOR-ALPHA FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10601">
        <property type="entry name" value="zf-LITAF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00714">
        <property type="entry name" value="LITAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51837">
        <property type="entry name" value="LITAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="122"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="LITAF" evidence="10">
        <location>
            <begin position="85"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="37"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8036916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="169" mass="18614" checksum="416556CBE8B0A253" modified="2023-09-13" version="1">MSDQPPPYPGTEKGSSPPYSENPAYPPTGQQGYSSFPQNYSQPGYPQQPQPAAGYTYQPYPQTIQPQQPTYPQGGYPVNTQPVAAGTTVTILQTCYRDVPVETQCPNCNNHVTTQVTYDAGAMAWLMCFIMCLVGLWCCCCIPFCVDGLKDADHHCPNCKHRIAHYSPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HDC3</accession>
    <name>A0A9Q1HDC3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_15529</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8041041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HDC3"/>
    <dbReference type="OrthoDB" id="6782434at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37162:SF10">
        <property type="entry name" value="DUF4371 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37162">
        <property type="entry name" value="HAT FAMILY DIMERISATION DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="121"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8041041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="263" mass="30009" checksum="018CF125AEDAC376" modified="2023-09-13" version="1">MFPDSNIAAKYGSRRTKTTAIARTLGTDTKDNAAVMMGPQRGVASFVKRENENVFILGCPCHLIHLAAEKGASQLPFSPVSLLIPIFYYLAKSKKSLNRFLEQWVALEQYFKEEAQGEMKRKNIPQVQKPKKSLSAAKDSSKSKQNFPEKSLKSVVKYFITVCDYIVQKLPLRDELLNHAKVANPSKRLEMSFTSVSFFVKRFHIMKDKLDELEIEFAHYQVENIPTELLQQKRVDSTWVALSHLKDESSGEFKYANLSTFFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HEF9</accession>
    <name>A0A9Q1HEF9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein yippee-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_09859</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the yippee family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8042960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HEF9"/>
    <dbReference type="OrthoDB" id="6407410at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034751">
        <property type="entry name" value="Yippee"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004910">
        <property type="entry name" value="Yippee/Mis18/Cereblon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039058">
        <property type="entry name" value="Yippee_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13848">
        <property type="entry name" value="PROTEIN YIPPEE-LIKE CG15309-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03226">
        <property type="entry name" value="Yippee-Mis18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51792">
        <property type="entry name" value="YIPPEE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Yippee" evidence="5">
        <location>
            <begin position="14"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU110713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8042960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="116" mass="13355" checksum="3248D35F64E96D60" modified="2023-09-13" version="1">MVKTFQAYLPNCHRRYSCIHCRAHLANHDDLISKSFQGSQGRAYLFNSVVNIGCGPAEERVLLTGLHAVSDIYCECCKTTLGWKYERAFENSQRYKEGKYIIELAHMTKDNGWECD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HEP0</accession>
    <name>A0A9Q1HEP0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Helix-turn-helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_05652</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HEP0"/>
    <dbReference type="OrthoDB" id="10018421at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058912">
        <property type="entry name" value="HTH_animal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21301">
        <property type="entry name" value="REVERSE TRANSCRIPTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21301:SF10">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26215">
        <property type="entry name" value="HTH_animal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helix-turn-helix" evidence="1">
        <location>
            <begin position="100"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF26215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8046842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="199" mass="23085" checksum="B4FDAC0AB5CBCCE8" modified="2023-09-13" version="1">MAPGYACLGMGLVEDKLWKSCPLKPSEWCRYIGDIWGLWPHGNEAFISFVGILNNLYPDELEYTYEFDFNTINFLDIRIQRSKEGFLSTGLFVKPTFKNLYLRYDSYHSMSTLDNIAYGQALRIKSFCSDERDVHRNLTDLHNNLSERSYPSSKSAIWDSEKERLVLIQLANEYTSTYISQGVELYGFVVYGLKHIRIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HFG1</accession>
    <name>A0A9Q1HFG1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_06881</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HFG1"/>
    <dbReference type="OrthoDB" id="6249720at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8047787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="101" mass="11253" checksum="53B736309B31EF2E" modified="2023-09-13" version="1">MNFAPAQSKIPTKELITATDNGIKDLSIGEANLIRCDVLRILKKAKMPKSNISHKEKVALSELKIDDSIIILPADKGRSTVVTNKEQYIEKMSNLLMEDKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HG26</accession>
    <name>A0A9Q1HG26_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ATP-dependent DNA helicase PIF1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_00831</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HG26"/>
    <dbReference type="OrthoDB" id="6152543at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049163">
        <property type="entry name" value="Pif1-like_2B_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051055">
        <property type="entry name" value="PIF1_helicase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47642">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47642:SF5">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21530">
        <property type="entry name" value="Pif1_2B_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 10" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA helicase Pif1-like 2B" evidence="9">
        <location>
            <begin position="41"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF21530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8048492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="382" mass="42809" checksum="4EEF537955D2479A" modified="2023-09-13" version="1">MLHSLQSRVEFIEAVDIIGTANATLKKLLKPTQNAKSTLPAELNIAIGARVMLTTNLDVADGLVNGVTGKVTGIVHGNLPNNQPEAIWILFDNPRIGANCRRKYLPPANVAQGSVVILPHKEIYQIRLQHVTRQQFPLKLAWAFTIHKVQGMTMERATVSFTGIFQAGMAYVALSRVVTLGGLYLTDFDSNLIYCNAEVQDSISNMPRCNLQSSNMLLHSEMNFSILHHNTQSLFCHYKDIISNPEFMKADIICLSETWLTSVKLHCIHIPGYNLHCSNQQARGQGVAIYICEKYRYTFIPLETGQCHSLLVATHTIPSFLILMLYRPPSITTTHFLSLLVKALNQMETCHQIYNTDYKVILGDFNYDLLKLPSLGPLKNNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HH21</accession>
    <name>A0A9Q1HH21_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_04895</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HH21"/>
    <dbReference type="OrthoDB" id="203440at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8046274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="51" mass="5922" checksum="64230CCE626ECF05" modified="2023-09-13" version="1">MFVDSALDIHELTNTINSYIGFCVDLVIPQETVKCYPNNKPWITKEIKYIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HHC6</accession>
    <name>A0A9Q1HHC6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Bone morphogenetic protein 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_07858</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the TGF-beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HHC6"/>
    <dbReference type="OrthoDB" id="6135292at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13756">
        <property type="entry name" value="TGF_beta_BMPs_GDFs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001839">
        <property type="entry name" value="TGF-b_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015615">
        <property type="entry name" value="TGF-beta-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017948">
        <property type="entry name" value="TGFb_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848">
        <property type="entry name" value="TGF-BETA FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00019">
        <property type="entry name" value="TGF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00204">
        <property type="entry name" value="TGFB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00250">
        <property type="entry name" value="TGF_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51362">
        <property type="entry name" value="TGF_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 6" id="KW-0339">Growth factor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="TGF-beta family profile" evidence="8">
        <location>
            <begin position="168"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="147"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="150"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8044953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="294" mass="33210" checksum="22E88D2B58404ED3" modified="2023-09-13" version="1">MYDEKFTSRQQFYLVFTQNQPAGANPNPNGTTHNEDWERSEEVVVEVADESSFTNDFGARGKYVEHAGFHIGKYQVYNATEIIPYARAGNGSQLVIKASVHLGNLSVEVKNFIVTTPGNAKSPLLVAFDNPHLESYIRFLTQNRGQPIEGYEHPPNSHDDGLTTPDKRPKRETKDDCRLRDWYVSFNDINWRELVYVPAGYSANFCHGSCHLGSPLRVLNATWPVSNHALLRSLFRHQMRRSNLSGPQELPPHSCCVPVQFEPLTMLYLEMSEPTSFYVLKSVPNMVATKCGCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HHE6</accession>
    <name>A0A9Q1HHE6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_09012</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HHE6"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8045900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="94" mass="11220" checksum="9A5AEE88B06AA129" modified="2023-09-13" version="1">MITSSSWCYKTTRQEHRIESIDERMESSREFNFNAAFLELSRSENCLRYVPIIAATWQHFEEAAIVVIQLLCVLEVSIKERSRNYWTVYTRSFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HI55</accession>
    <name>A0A9Q1HI55_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_08879</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HI55"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8045796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="66" mass="7535" checksum="C164C9CBD94DA7A3" modified="2023-09-13" version="1">MLLFLALYKHRIPTTAFRITERHQFAIFSGHKSVVDMDTAVKQAMNGKGVPVLNKTAENNFLWRSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HJJ8</accession>
    <name>A0A9Q1HJJ8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Octopamine receptor beta-3R</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_06317</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HJJ8"/>
    <dbReference type="OrthoDB" id="10071887at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032870">
        <property type="term" value="P:cellular response to hormone stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24241">
        <property type="entry name" value="NEUROPEPTIDE RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24241:SF76">
        <property type="entry name" value="NEUROPEPTIDE SIFAMIDE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8" id="KW-0675">Receptor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="7">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8047338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="108" mass="11988" checksum="6DD3BD07C02DC9A4" modified="2023-09-13" version="1">MVIVMGAFVICWTPSIFKLLFAVYGSYSVKTLDVIQVSAEILSVANSTVNPIIYGYRNSSFRAAYKNVITKYCSCIFRKNTANLKCDAKVTEQSCLDFEVSKISTSAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HKU3</accession>
    <name>A0A9Q1HKU3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_04466</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8051044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HKU3"/>
    <dbReference type="OrthoDB" id="10066002at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="95"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8051044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="120" mass="13525" checksum="9274AAED976AC88D" modified="2023-09-13" version="1">MVTREQRALKLCKFLRVGTNLPECLETISQSQSFVLILGERSIPEQTFVVLESQTVTGISLLQAVDICFKLFLFVGCQVYACMGMLKDLEFLPEIYLLTCSVAIKIFSTGNYVIIWVFDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HKW4</accession>
    <name>A0A9Q1HKW4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Bcl-2-related protein A1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_01354</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Bcl-2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HKW4"/>
    <dbReference type="OrthoDB" id="6021377at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051400">
        <property type="term" value="F:BH domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097192">
        <property type="term" value="P:extrinsic apoptotic signaling pathway in absence of ligand"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008630">
        <property type="term" value="P:intrinsic apoptotic signaling pathway in response to DNA damage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001836">
        <property type="term" value="P:release of cytochrome c from mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.437.10">
        <property type="entry name" value="Blc2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036834">
        <property type="entry name" value="Bcl-2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046371">
        <property type="entry name" value="Bcl-2_BH1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026298">
        <property type="entry name" value="Bcl-2_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002475">
        <property type="entry name" value="Bcl2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11256">
        <property type="entry name" value="BCL-2 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11256:SF47">
        <property type="entry name" value="BCL-2-LIKE PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00452">
        <property type="entry name" value="Bcl-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56854">
        <property type="entry name" value="Bcl-2 inhibitors of programmed cell death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50062">
        <property type="entry name" value="BCL2_FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Bcl-2 Bcl-2 homology region 1-3" evidence="7">
        <location>
            <begin position="6"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8048868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="114" mass="13352" checksum="813591FB793FF7B8" modified="2023-09-13" version="1">MVNHVRQCDKETIKATLDAMFEDGLYSWGIIVMTYVFIRKCAQRSREQGKDKDTIEQLAQFVGEYVEDRCTQWIEQREGWDDFCQTFMKNTHNSRATAIKPMVIAVVTMTLAYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HLH5</accession>
    <name>A0A9Q1HLH5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NLR family CARD domain-containing protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_02568</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HLH5"/>
    <dbReference type="OrthoDB" id="427518at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312">
        <property type="entry name" value="NACHT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312:SF2">
        <property type="entry name" value="NUCLEOTIDE-BINDING OLIGOMERIZATION DOMAIN-CONTAINING PROTEIN 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NACHT" evidence="1">
        <location>
            <begin position="32"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8049708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="469" mass="54474" checksum="42121B7021D247B7" modified="2023-09-13" version="1">MRVKNNKKSSKDPTCWTFVDSRHKLLQRTDEGTIVIEGEAGFGKSILLTQYAYDWYSSTADSPLKKDTIFILLRLREFKKSGSIFETIKLLLLNDLSLSCNEIEDIITSQSWKVVFAFDGYDEFKYRDSTHVDVLKIIHGRMLTSSLVIVSTRPECWTKLQNSAEIRCRLTGFDRSIQNMYIEKVIIPNTEKRDIKTLISQTLQANGFLSDICQVPLFFVIFAHMKYTNHDELNITTVTGYFKYMLSCSLIHSKSKETDETKSRFTRQEENYYQASMAKFAFDGLFSQNFVWEKTEMMRLVDLSCYTYYIKAGIILEEHNSRYKVHPGCHMDDESVDEINVRFFHKTFQEYYAAIHFVYLVSKERSMDVSAILSKFYENELQYFFQFACGLKPTSAKVVQDYLGSLRGGQILSILCSVEQETQDTGKIVESFSRRTTIDWLDSRIHVSAMIQILQIASHQRVGLPRSVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HLJ7</accession>
    <name>A0A9Q1HLJ7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_02834</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HLJ7"/>
    <dbReference type="OrthoDB" id="10069752at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46704">
        <property type="entry name" value="CXC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46704:SF1">
        <property type="entry name" value="TELOMERE LENGTH REGULATION PROTEIN TEL2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8049891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1135" mass="128389" checksum="5BA4914639830E04" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HME4</accession>
    <name>A0A9Q1HME4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Reverse transcriptase RNase H-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_04631</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8051166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HME4"/>
    <dbReference type="OrthoDB" id="5985335at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041373">
        <property type="entry name" value="RT_RNaseH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF13">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17917">
        <property type="entry name" value="RT_RNaseH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Reverse transcriptase RNase H-like" evidence="7">
        <location>
            <begin position="2"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF17917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8051166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="65" mass="7274" checksum="4B07E13315AF7FF1" modified="2023-09-13" version="1">MSCDASPYGIDVVLSHQMEDGQERPIEFASRALTAAEERYSQFDKEGLAIIFGVTNFYKYLYGSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0Y8H9</accession>
    <name>A0A9Q0Y8H9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HIN-200 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_44920</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010001460">
        <property type="protein sequence ID" value="KAJ8017573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0Y8H9"/>
    <dbReference type="OrthoDB" id="8838938at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004021">
        <property type="entry name" value="HIN200/IF120x"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02760">
        <property type="entry name" value="HIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HIN-200" evidence="1">
        <location>
            <begin position="21"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8017573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="226" mass="25065" checksum="6AD5FB8948FEED55" modified="2023-09-13" version="1">MFTIKDLLTLRPAPYTKMFKCKVLVISAPKPFSTKGEQKELYHIALGDQTGLIKATLYDEDKLKQMKVGSSVMLTNYIVKADKSVTITSASKVFRCADIDVDDVTQDSAQQLINPAPQKVCKIKLIKSSPKKTLTSLQGQITKDEAAKQVMVRGTPSAVRNIFVKDETDEIEVALWRSSAETDATVNDWIEVTNVVVNEPNTYHTQTFVATTQNTTIKVSSKSVVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0Y977</accession>
    <name>A0A9Q0Y977_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Carbamoyl-phosphate synthase [ammonia], mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_45124</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010001895">
        <property type="protein sequence ID" value="KAJ8017455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0Y977"/>
    <dbReference type="OrthoDB" id="434at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8017455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="50" mass="5611" checksum="2DB12D45EF366613" modified="2023-09-13" version="1">MVMAIGRTFEESLQKAVRMIHPSIKRFAPEMPVGKVMPKDFRGCYGGAIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YAE8</accession>
    <name>A0A9Q0YAE8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_42440</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000073">
        <property type="protein sequence ID" value="KAJ8019162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YAE8"/>
    <dbReference type="OrthoDB" id="10066550at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21301:SF11">
        <property type="entry name" value="GIY-YIG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21301">
        <property type="entry name" value="REVERSE TRANSCRIPTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8019162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="207" mass="23746" checksum="4CEB552DA824B80E" modified="2023-09-13" version="1">MEDKTYRPLKRDPTTSLENEIGKAIKELKEQNKLNKKQATQLTPRNSLPSRIYGLPKVHKEGIPLRPIVSSINSPSYNLARHLADILPPLSGKRMSYIKNSQHFVERAKKISIETTDILVSFDVVSLFTNVPINDACKLIGDRLQADTTLHNRTQMTWEEIVHLTNLCLNSTYFKWHDQFYEQCEGQAMGSPCLLSRLISLWSILRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YCJ1</accession>
    <name>A0A9Q0YCJ1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_45178</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010002033">
        <property type="protein sequence ID" value="KAJ8017427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YCJ1"/>
    <dbReference type="OrthoDB" id="6512834at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31025">
        <property type="entry name" value="SI:CH211-196P9.1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31025:SF9">
        <property type="entry name" value="SI:DKEY-286J15.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8017427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="302" mass="35556" checksum="185DD8EA4F3803CF" modified="2023-09-13" version="1">MYSLRPGVIQKLRSGDPLSRSDRSNITEACYQHVTKFTYYPKEWQYTEVVTAFLEEFPKVKDKDGIEVASNLWRQRIQYRFQNDRNRRDKNIPMKRKLSKDDVPSQRKGKAIIWGLANYLPVRPEGEDDDSILRHEAALKTEMVKRHPDHGRLDRLMALTFSDRRNFIINENPLVCEIKEKYPALFESENAVLKEFRRIAGKEMEELICEGSKKYHQKLMGLAKTQENELLQILLEHHSSITDDEEIKDLYCCVSLLMVPLMLKERVEDIVRNEVSNMKCLKVIKERFQFGTILTIADRFVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YCN2</accession>
    <name>A0A9Q0YCN2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NLR family CARD domain-containing protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_42754</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000129">
        <property type="protein sequence ID" value="KAJ8018960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YCN2"/>
    <dbReference type="OrthoDB" id="120976at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312">
        <property type="entry name" value="NACHT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312:SF2">
        <property type="entry name" value="NUCLEOTIDE-BINDING OLIGOMERIZATION DOMAIN-CONTAINING PROTEIN 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="493"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="domain" description="Immunoglobulin" evidence="2">
        <location>
            <begin position="45"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Immunoglobulin" evidence="2">
        <location>
            <begin position="265"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8018960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1045" mass="118606" checksum="676664F7BB317EF0" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YF86</accession>
    <name>A0A9Q0YF86_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Ras-related GTP-binding protein C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HOLleu_40030</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Nanhai2018</strain>
            <tissue evidence="15">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Lysosome membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the GTR/RAG GTP-binding protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YF86"/>
    <dbReference type="OrthoDB" id="26136at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990131">
        <property type="term" value="C:Gtr1-Gtr2 GTPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019899">
        <property type="term" value="F:enzyme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019003">
        <property type="term" value="F:GDP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010507">
        <property type="term" value="P:negative regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043200">
        <property type="term" value="P:response to amino acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11385">
        <property type="entry name" value="RagC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.190:FF:000001">
        <property type="entry name" value="Ras-related GTP-binding protein C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000226">
        <property type="entry name" value="Ras-related GTP-binding protein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006762">
        <property type="entry name" value="Gtr1_RagA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039400">
        <property type="entry name" value="RagC/D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11259:SF2">
        <property type="entry name" value="GH16429P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11259">
        <property type="entry name" value="RAS-RELATED GTP BINDING RAG/GTR YEAST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04670">
        <property type="entry name" value="Gtr1_RagA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 13" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0458">Lysosome</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="359"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="364"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU367014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ8020441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="383" mass="42584" checksum="6D5151A522D24862" modified="2023-09-13" version="1">MSLPDDGGIVGSYGVVGSFPKDFDYGPDDHDMENGTVPPDNRPRILLMGLRRSGKSSIQKVVFHKMSPNETLFLESTNKIVKSDVNSTSFVQFQIWDFPGQIDFFDSTFDSETIFGGCGALVFVIDAQDDYMDALVRLNMTVSRAFKVNPNIKFEVFIHKVDGLSDDHKIETQRDIHQRATDDLADAGLEGVHLSFYLTSIYDHSIFEAFSKVVQKLVPQLGTLENLLDILISNSCIEKAFLFDVDSKIYIATDSSPVDMQSYELCCDMIDVVIDVSCIYGLKEDGEGTAYDAESSSVIRLNNGTILYLKEVNRYLALVCILREDSFDRQGLIDYNFLCFKKAIEEVFEVRQKTVIGSNGGGQRLSTDSFGSPKTNGAVAAKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YG44</accession>
    <name>A0A9Q0YG44_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">TTF-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_41161</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8019527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YG44"/>
    <dbReference type="OrthoDB" id="10059235at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8019527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="163" mass="19085" checksum="DF22BA3C2B628584" modified="2023-09-13" version="1">MQTCTSSSVPNANISDQSQPFNDEPGVPPGPHQPFEFNFAARIYGKQNRAFQTHWFRSFPWLHYLEINDSVLCYTCITADKQKKLINARNSEKAFLVEGFWNWNKALEKFRDHQKCTCHRLAVEHAESPRIYGDIGEKMNATLAEERKKNRTIFIKTEKSWRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-06-18" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YG51</accession>
    <name>A0A9Q0YG51_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Cytosolic carboxypeptidase 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_40472</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the peptidase M14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10253041at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.3120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050821">
        <property type="entry name" value="Cytosolic_carboxypeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040626">
        <property type="entry name" value="Pepdidase_M14_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000834">
        <property type="entry name" value="Peptidase_M14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12756">
        <property type="entry name" value="CYTOSOLIC CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12756:SF4">
        <property type="entry name" value="PEPTIDASE M14 CARBOXYPEPTIDASE A DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18027">
        <property type="entry name" value="Pepdidase_M14_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00246">
        <property type="entry name" value="Peptidase_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52035">
        <property type="entry name" value="PEPTIDASE_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M14" evidence="5">
        <location>
            <begin position="559"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="246"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="890"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="988"/>
            <end position="1079"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1423"/>
            <end position="1446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="27"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="247"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="891"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="917"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="988"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1019"/>
            <end position="1031"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1054"/>
            <end position="1067"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="3">
        <location>
            <position position="797"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS52035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8020790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1446" mass="164652" checksum="3529C832BC9FDD81" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YG67</accession>
    <name>A0A9Q0YG67_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Spermidine synthase</fullName>
            <ecNumber evidence="4">2.5.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Putrescine aminopropyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_39163</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM). Has a strong preference for putrescine as substrate, and has very low activity towards 1,3-diaminopropane. Has extremely low activity towards spermidine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>S-adenosyl 3-(methylsulfanyl)propylamine + putrescine = S-methyl-5'-thioadenosine + spermidine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12721"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:57443"/>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
            <dbReference type="ChEBI" id="CHEBI:326268"/>
            <dbReference type="EC" id="2.5.1.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amine and polyamine biosynthesis; spermidine biosynthesis; spermidine from putrescine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer or homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the spermidine/spermine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.16" evidence="4"/>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YG67"/>
    <dbReference type="OrthoDB" id="38125at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004766">
        <property type="term" value="F:spermidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008295">
        <property type="term" value="P:spermidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.140.10:FF:000001">
        <property type="entry name" value="SPE3p Spermidine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000013">
        <property type="entry name" value="Spermidine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.140.10">
        <property type="entry name" value="Spermidine synthase, tetramerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00198">
        <property type="entry name" value="Spermidine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030374">
        <property type="entry name" value="PABS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030373">
        <property type="entry name" value="PABS_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001045">
        <property type="entry name" value="Spermi_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030668">
        <property type="entry name" value="Spermi_synthase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035246">
        <property type="entry name" value="Spermidine_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037163">
        <property type="entry name" value="Spermidine_synt_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002010">
        <property type="entry name" value="PRK00811.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00417">
        <property type="entry name" value="speE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11558:SF11">
        <property type="entry name" value="SPERMIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11558">
        <property type="entry name" value="SPERMIDINE/SPERMINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17284">
        <property type="entry name" value="Spermine_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01564">
        <property type="entry name" value="Spermine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000502">
        <property type="entry name" value="Spermidine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01330">
        <property type="entry name" value="PABS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51006">
        <property type="entry name" value="PABS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PABS" evidence="12">
        <location>
            <begin position="6"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00053963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00072554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00082964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8021850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="291" mass="32717" checksum="4FD161761F1D1763" modified="2023-09-13" version="1">MDLIKDGWFSEVSTLWPGQAMSLQVEEVLHHEKSKYQDVLVFKSKSHGNVLVLDGVIQVTERDEFSYQEMMTHVPINCHHDPKKVLIVGGGDGGAAREVLKHKNIESVTLCEIDEEVIEVSKKYLPGLASSFSNPRLTVHIGDGFEFMKKNNGAFDIIITDSSDPIGPAESLFEKSYYSLMKSALKPNGIICSQGECLWLHLEIITKMQTFCRSLFPAVDYGYCTIPTYPSGQIGFTLCSLNPDTNFKEPLRICGPEEVEEFDLRYYNSDIHKAAFILPEFARKALIPQSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YH54</accession>
    <name>A0A9Q0YH54_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">ATP-binding cassette sub-family G member 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_37291</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YH54"/>
    <dbReference type="OrthoDB" id="66620at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015562">
        <property type="term" value="F:efflux transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008514">
        <property type="term" value="F:organic anion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03213">
        <property type="entry name" value="ABCG_EPDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000622">
        <property type="entry name" value="ATP-binding cassette sub-family G member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050352">
        <property type="entry name" value="ABCG_transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041:SF116">
        <property type="entry name" value="PROTEIN BROWN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="399"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="434"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="510"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="540"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="634"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="50"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="21"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8022405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="658" mass="73074" checksum="E03045CC3FBC0592" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YHD8</accession>
    <name>A0A9Q0YHD8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Orexin receptor type 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_37604</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YHD8"/>
    <dbReference type="OrthoDB" id="5969463at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008528">
        <property type="term" value="F:G protein-coupled peptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007218">
        <property type="term" value="P:neuropeptide signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00637">
        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24230">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24230:SF75">
        <property type="entry name" value="RELAXIN FAMILY PEPTIDE RECEPTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="41"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="78"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="119"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="160"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="284"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="319"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="22"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="227"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="231"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="250"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8022642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="362" mass="41665" checksum="B127FF5B602A6052" modified="2023-09-13" version="1">MEIYQWLMIFYFAILFLVGSIANILLLAIYWKNKNHSSVIVFIKALAITDFYVCFTKPVGIFYWLLQDYSLDNSFCKVFQFTIGTGYTLTFCMIFLVALDRYLAIYHTEGSFSVTAQHAMILVAAAWLLCHVGNIIKIMLSDDLQLQFGKYFCAERADGSVYVMYGFLAFVFVLCSMVVLWLNFQIIQALKRQSQTKPEGSIPRINQKMQGLSRAEVFKGTSEEQQQAKLVDLESHDSPKKGKSEKMPSKQLTRTTTSKTGHHQKEGKPVKAKKSKVITSLTKMLAVVTLLYVATSLPSIIIYFVPLDSEFWIAQQSPILYALYLLLKYLFFVDSIVKPFIYYAISRTFREQCRDFFKQLRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YI53</accession>
    <name>A0A9Q0YI53_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_38047</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YI53"/>
    <dbReference type="OrthoDB" id="5948273at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912">
        <property type="entry name" value="IP13529P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912:SF34">
        <property type="entry name" value="NOTOCHORD-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8022993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="806" mass="91550" checksum="943C971BA9D95CA3" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YI57</accession>
    <name>A0A9Q0YI57_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Follistatin-related protein 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_37509</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YI57"/>
    <dbReference type="OrthoDB" id="6085115at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030510">
        <property type="term" value="P:regulation of BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00104">
        <property type="entry name" value="KAZAL_FS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000032">
        <property type="entry name" value="palladin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036058">
        <property type="entry name" value="Kazal_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050653">
        <property type="entry name" value="Prot_Inhib_GrowthFact_Antg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011044">
        <property type="entry name" value="Quino_amine_DH_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10913">
        <property type="entry name" value="FOLLISTATIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10913:SF81">
        <property type="entry name" value="KAZAL-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07648">
        <property type="entry name" value="Kazal_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00280">
        <property type="entry name" value="KAZAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100895">
        <property type="entry name" value="Kazal-type serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50969">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51465">
        <property type="entry name" value="KAZAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Kazal-like" evidence="7">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="148"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="218"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="314"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8022569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="810" mass="91216" checksum="B8D92662113A3A3C" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YIW7</accession>
    <name>A0A9Q0YIW7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_38100</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8023034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YIW7"/>
    <dbReference type="OrthoDB" id="5978902at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2330">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8023034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="225" mass="25658" checksum="D631FB607761F090" modified="2023-09-13" version="1">MKYYDISVHDNGENIYDHFYRTIQYKLYYPLLFSLPFNYSKQTISSQENFLFNIAGGLQEVKIKIQNSVIKGYHEYKIAPPIELPLRLTPQYANIHDEEAVLVWMPNEHSINEEILKSKNSQGITIKELVNPVSVIIGHVPRGLGRLLKQCMDKNVQVTCLATGKPRQSFPPWPHFSQKGGGVVIPCEYILTCKKNEAQELISKIKSSFQNVQGGEGMKISVINM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YJY1</accession>
    <name>A0A9Q0YJY1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein charybde</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_37720</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DDIT4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YJY1"/>
    <dbReference type="OrthoDB" id="10018535at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009968">
        <property type="term" value="P:negative regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032006">
        <property type="term" value="P:regulation of TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.40">
        <property type="entry name" value="RTP801-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012918">
        <property type="entry name" value="RTP801-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038281">
        <property type="entry name" value="RTP801-like_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12478">
        <property type="entry name" value="DNA-DAMAGE-INDUCIBLE TRANSCRIPT 4 PROTEIN DDIT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12478:SF16">
        <property type="entry name" value="PROTEIN CHARYBDE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07809">
        <property type="entry name" value="RTP801_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8022736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="186" mass="21052" checksum="13FE3DA8A33F7A16" modified="2023-09-13" version="1">MKVFEVAQVVHKPELFTMVVPVIENGKHLNISDNENSEDVSLNLDDLQDFKLTDYISSLLLDTLRTAKDRVPNLQILVPSDLAEHIARDVVCMSGNEPCGARGCALDLIIEDGEMCTRIGKCQVDPLTVTTFEVTVVLQKDVPKWFPKLQMILRNSESKTVIKPTYKLVKRKLYRKESKIHVQESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YLL2</accession>
    <name>A0A9Q0YLL2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">COP9 signalosome complex subunit 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_37237</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CSN4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YLL2"/>
    <dbReference type="OrthoDB" id="295656at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008180">
        <property type="term" value="C:COP9 signalosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041406">
        <property type="entry name" value="CSN4_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040134">
        <property type="entry name" value="PSMD12/CSN4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10855">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 12/COP9 SIGNALOSOME COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10855:SF2">
        <property type="entry name" value="COP9 SIGNALOSOME COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18420">
        <property type="entry name" value="CSN4_RPN5_eIF3a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0736">Signalosome</keyword>
    <feature type="domain" description="PCI" evidence="4">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8022362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="145" mass="16617" checksum="E62F47ED604B16B9" modified="2023-09-13" version="1">MLATLFKDERCQQLPAYDILKKMYLERIIRGDQLQEFAAMLQPHQKATTADASKLYNNIKFEELGALLEIPPSKAEKIASQMISEGRMNGYIDQIDGIVHFESPETLPQWDKQIQSLCLQVNNIIEKINLHAPEWTAAISENQMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YLN8</accession>
    <name>A0A9Q0YLN8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Retroviral-like aspartic protease 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_34635</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YLN8"/>
    <dbReference type="OrthoDB" id="6161062at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00303">
        <property type="entry name" value="retropepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13975">
        <property type="entry name" value="gag-asp_proteas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8024669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="205" mass="22487" checksum="E1F8875D85470918" modified="2023-09-13" version="1">MAQWCLYIYIYIYIYIYISSISGKRAGAGPVGPTPAQDRRNQGPYNDSSSPTDQTSKKTSVNLDPAGMYLPVGIYSKIVPFLIDSGSSITLVSKETFENLPEEIKQTRKKGKKTLWLADGSQVENKGTLRLDMEVGGAVLEHEVHIADIGSDAILGLDFLMNHHCLLDWNNGVIKLNGKDVALQSKKGQAGIFRVKVTETVIVPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YMV7</accession>
    <name>A0A9Q0YMV7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Arylsulfatase J</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_39057</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sulfatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YMV7"/>
    <dbReference type="OrthoDB" id="103349at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008484">
        <property type="term" value="F:sulfuric ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030317">
        <property type="term" value="P:flagellated sperm motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16029">
        <property type="entry name" value="4-S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.720.10:FF:000007">
        <property type="entry name" value="Arylsulfatase family, member J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047115">
        <property type="entry name" value="ARSB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054709">
        <property type="entry name" value="CFAP107"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024607">
        <property type="entry name" value="Sulfatase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000917">
        <property type="entry name" value="Sulfatase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10342">
        <property type="entry name" value="ARYLSULFATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10342:SF274">
        <property type="entry name" value="ARYLSULFATASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22595">
        <property type="entry name" value="CFAP107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00884">
        <property type="entry name" value="Sulfatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00149">
        <property type="entry name" value="SULFATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sulfatase N-terminal" evidence="7">
        <location>
            <begin position="241"/>
            <end position="558"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8021766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="715" mass="81142" checksum="0DB0CBAD8D2E4641" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YN29</accession>
    <name>A0A9Q0YN29_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_33070</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YN29"/>
    <dbReference type="OrthoDB" id="10419931at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040474642" evidence="1">
        <location>
            <begin position="25"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8025495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="229" mass="26433" checksum="F55EB1459B75EF82" modified="2023-09-13" version="1">MAVEAKIVFFSLVVCIWFASFVTCQTITGRPQDYGEISKAVDRFLDILVKEDTTDPDFKLDSWETLLDEEINPKLKILAHVMRSLSSRPDIPSVREQLYIPTSYLQPFITDEDAYSNDPSSKLPSLQSSGLDQIKNKERNNYWGESDIFPENEEKRNGIWYGKRATNTDDAATKRNGIWYGKRNSPTIEDKRNGIWYGKRNGIWYGKRNGIWYGKRDSGYYPSVADEVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YP80</accession>
    <name>A0A9Q0YP80_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_33747</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8026019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YP80"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8026019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="116" mass="13534" checksum="3E4D135026F35A86" modified="2023-09-13" version="1">MHTTSEVACDIRKRKMEDDGTLKAPPAKQIQKADGSKCPEKNSEVKSNSPKNPWGPIFKRPMLYIHLFGQFILRYNKDSCMLKADVMAYVNFLNEFQRFRYYPLCKYCSFLNVKLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YR23</accession>
    <name>A0A9Q0YR23_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_34747</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YR23"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8024747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="104" mass="11519" checksum="D574444A5CACAD99" modified="2023-09-13" version="1">MPHIERKNPIVFGGGQRSSGVTRGQTLKTLLTQYLKMRYGKRKNPIVFDGGQRSSGVTGGQTLKTLLTRYLKMCYGERKNPIVFGGSQRSCGVTRGQILKTLLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YR29</accession>
    <name>A0A9Q0YR29_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Low-density lipoprotein receptor-related protein 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HOLleu_36231</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Nanhai2018</strain>
            <tissue evidence="15">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="72419at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000001">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000241">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 6"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050778">
        <property type="entry name" value="Cueball_EGF_LRP_Nidogen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000033">
        <property type="entry name" value="LDLR_classB_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46513:SF41">
        <property type="entry name" value="LOW-DENSITY LIPOPROTEIN RECEPTOR-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46513">
        <property type="entry name" value="VITELLOGENIN RECEPTOR-LIKE PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14670">
        <property type="entry name" value="FXa_inhibition"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00058">
        <property type="entry name" value="Ldl_recept_b"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00261">
        <property type="entry name" value="LDLRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00135">
        <property type="entry name" value="LY"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63825">
        <property type="entry name" value="YWTD domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51120">
        <property type="entry name" value="LDLRB"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="3" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 15" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1404"/>
            <end position="1426"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="11">
        <location>
            <begin position="157"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="11">
        <location>
            <begin position="201"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="14">
        <location>
            <begin position="293"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="11">
        <location>
            <begin position="379"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="11">
        <location>
            <begin position="422"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="11">
        <location>
            <begin position="465"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="14">
        <location>
            <begin position="599"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="11">
        <location>
            <begin position="682"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="14">
        <location>
            <begin position="905"/>
            <end position="947"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="11">
        <location>
            <begin position="1049"/>
            <end position="1091"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="14">
        <location>
            <begin position="1230"/>
            <end position="1266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1493"/>
            <end position="1538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1564"/>
            <end position="1656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1508"/>
            <end position="1521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="1591"/>
            <end position="1602"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="1292"/>
            <end position="1307"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="1310"/>
            <end position="1322"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="1317"/>
            <end position="1335"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="1329"/>
            <end position="1344"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="1349"/>
            <end position="1361"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="1369"/>
            <end position="1384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ8023716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1656" mass="185838" checksum="780F7AFBFB1DAF1D" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YRB6</accession>
    <name>A0A9Q0YRB6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Armadillo repeat-containing protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_32108</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YRB6"/>
    <dbReference type="OrthoDB" id="247006at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044782">
        <property type="term" value="P:cilium organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038905">
        <property type="entry name" value="ARMC2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21356">
        <property type="entry name" value="ARMADILLO REPEAT CONTAINING 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21356:SF1">
        <property type="entry name" value="ARMADILLO REPEAT-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ARM" evidence="1">
        <location>
            <begin position="719"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="22"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="34"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="115"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="138"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="169"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="191"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="227"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8027073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="874" mass="97055" checksum="72C2474C32AA05A9" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YS85</accession>
    <name>A0A9Q0YS85_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Toll-like receptor 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_34512</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YS85"/>
    <dbReference type="OrthoDB" id="9982425at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042342">
        <property type="entry name" value="TTC22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16253">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16253:SF1">
        <property type="entry name" value="TIR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13676">
        <property type="entry name" value="TIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0675">Receptor</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TIR" evidence="1">
        <location>
            <begin position="480"/>
            <end position="614"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8024571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="614" mass="70736" checksum="7B9D8476A0ADA01A" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YTJ3</accession>
    <name>A0A9Q0YTJ3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HAT C-terminal dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_32024</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YTJ3"/>
    <dbReference type="OrthoDB" id="6617140at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8027014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="237" mass="27226" checksum="792D436D529F0864" modified="2023-09-13" version="1">MAKFYLGMKASLPVLTMMESFNRSLQAKDQTVECMIQCAKILRDQLTQMRNEEDFSVLFTRVSGFIEKIDEMEGFCQPRRRKVSGKLGSGFVAPEQTVEEQFRSQYFAAIDSAIVNLDTYFSTTDIHKYEQIVNMLITGKVNVAVVQSYPELSDGLELELQFFHRQYLSQCKPSLEQVHTIMRDMSHQVRQMFPWVEPLLRLILVSPASSCTAERIFQCPSKNQNMASKHNGTDVLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YUJ5</accession>
    <name>A0A9Q0YUJ5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Insulin-like growth factor-binding protein complex acid labile subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_32333</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YUJ5"/>
    <dbReference type="OrthoDB" id="6359842at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002224">
        <property type="term" value="P:toll-like receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24365:SF541">
        <property type="entry name" value="PROTEIN TOLL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24365">
        <property type="entry name" value="TOLL-LIKE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040480633" evidence="10">
        <location>
            <begin position="18"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="398"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8027241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="457" mass="51336" checksum="8A877490783F5973" modified="2023-09-13" version="1">MMFFVTTLLTYAVGVRSLPLTTTHRSNTVSVLRLHGDQAIPTCSEICDYSHSKAHCHDRNLFQIPHSTGCEGAILLELQGNKIISITSEQMSGYQQVSTLDISGNYLSNIIPGTFDRNSNLKNIDVSNNDLTILNSKMFKGTEHSLFQIEIQFNSINLVTQDAFEGLECVTNIYLSHNKLQNLLPGTFKDLISLQYLDLSQNKLAKLPQNLFHNSTSLRKLYLHKNKLQQIPNRLFDGLESLTDVRLSDNELISVPAPQELGLFHQLWRMDLQNNKIHQSGVILPFLANVTENLQVTGNPLLCDCNFQILQVSLHFLGGSHAQPIQCMYHNESNFTTSYTLPFDCYVTTQTHPTPSNNSTDKLLSASTHDGSHFDDIMSASPPEYPKCKGDVTPHYRIYVIVICALIILIAGLIIFICLICFVPSIRSKFILFVSRGTKELREQRQSGSDSSAERKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1B958</accession>
    <name>A0A9Q1B958_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Protoporphyrinogen oxidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_45006</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010001612">
        <property type="protein sequence ID" value="KAJ8017525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1B958"/>
    <dbReference type="OrthoDB" id="419752at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13450">
        <property type="entry name" value="NAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8017525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="97" mass="10780" checksum="01AA5454A309E5AD" modified="2023-09-13" version="1">MQKRHNMQRTVRCQLGLLVVDDSFCEAMTKPIDEHTCMSLNCSPVLLCLQVVLLEANTRLGGWIHSNHTQHGSVHELGTRSIRPAGLVGKNTLDMVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BAP5</accession>
    <name>A0A9Q1BAP5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="16">A disintegrin and metalloproteinase with thrombospondin motifs 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HOLleu_41404</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Nanhai2018</strain>
            <tissue evidence="16">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8019715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="10035764at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000001">
        <property type="entry name" value="semaphorin-5A isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1620.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="3"/>
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        <property type="entry name" value="ADAMTS/ADAMTS-like"/>
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    <dbReference type="InterPro" id="IPR050439">
        <property type="entry name" value="ADAMTS_ADAMTS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041645">
        <property type="entry name" value="ADAMTS_CR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045371">
        <property type="entry name" value="ADAMTS_CR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010294">
        <property type="entry name" value="ADAMTS_spacer1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001590">
        <property type="entry name" value="Peptidase_M12B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723:SF311">
        <property type="entry name" value="ADAM CYSTEINE-RICH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723">
        <property type="entry name" value="ADAMTS A DISINTEGRIN AND METALLOPROTEASE WITH THROMBOSPONDIN MOTIFS PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17771">
        <property type="entry name" value="ADAMTS_CR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19236">
        <property type="entry name" value="ADAMTS_CR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05986">
        <property type="entry name" value="ADAMTS_spacer1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01421">
        <property type="entry name" value="Reprolysin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19030">
        <property type="entry name" value="TSP1_ADAMTS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00090">
        <property type="entry name" value="TSP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01857">
        <property type="entry name" value="ADAMTSFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50215">
        <property type="entry name" value="ADAM_MEPRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="8 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 11 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 11 13" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M12B" evidence="15">
        <location>
            <begin position="79"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="14">
        <location>
            <begin position="786"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="active site" evidence="10 13">
        <location>
            <position position="229"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 13">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 13">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 13">
        <location>
            <position position="238"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="286"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="286"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="159"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="206"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="244"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="310"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="321"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="330"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="354"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="389"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="393"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="404"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR613273-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR613273-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR613273-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ8019715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="900" mass="98571" checksum="FAD2D6E0C3A59758" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BAZ3</accession>
    <name>A0A9Q1BAZ3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Carbamoyl-phosphate synthase [ammonia], mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_43098</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000207">
        <property type="protein sequence ID" value="KAJ8018738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BAZ3"/>
    <dbReference type="OrthoDB" id="8186278at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004088">
        <property type="term" value="F:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CPAse_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058047">
        <property type="entry name" value="CPSase_preATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11405:SF53">
        <property type="entry name" value="CARBAMOYL-PHOSPHATE SYNTHASE [AMMONIA], MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11405">
        <property type="entry name" value="CARBAMOYLTRANSFERASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25596">
        <property type="entry name" value="CPSase_L_D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carbamoyl phosphate synthase ATP-binding" evidence="4">
        <location>
            <begin position="105"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS00867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8018738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="124" mass="13313" checksum="8D23EF32CBE1351C" modified="2023-09-13" version="1">MDVSKVLVLGSGSISIGHAGEFDYSGSQAIKALKALALSSQVLIEKSLLGWKKLEYEVVRDAADNCVTVCNMENLTTIRCVRHLGIVGECNIQYAPHPNSLEYCIIEVNARLSQSSALTSKATG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BAZ8</accession>
    <name>A0A9Q1BAZ8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA-directed DNA polymerase</fullName>
            <ecNumber evidence="2">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_43076</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 12">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="2"/>
    <dbReference type="EMBL" id="JAIZAY010000202">
        <property type="protein sequence ID" value="KAJ8018753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BAZ8"/>
    <dbReference type="OrthoDB" id="5871067at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.960.10">
        <property type="entry name" value="VSR Endonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004868">
        <property type="entry name" value="DNA-dir_DNA_pol_B_mt/vir"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33568">
        <property type="entry name" value="DNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33568:SF3">
        <property type="entry name" value="DNA-DIRECTED DNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03175">
        <property type="entry name" value="DNA_pol_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0235">DNA replication</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA-directed DNA polymerase family B mitochondria/virus" evidence="9">
        <location>
            <begin position="44"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8018753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="353" mass="41212" checksum="EBC007D6E0818855" modified="2023-09-13" version="1">MVCDLCIMEPFESECLVCEEKQVIFQGPNTKREFCEWLLAPPQNNSTCIVHNLKGFDGYFILQHLYDNGVVPEIITNGAKVMSIKLLRNSIRFIDSVNFLPMPLSKMPTTFGFNELKKGYFPHLFNTTENQTYIGHFPEASYYAPDVMSSEKRKDFFKWYETEKNKGLFRSLFMDLTCKEIDNNVEDEAEEGDGVVTEMCGVDPFKHCTTIASACNLVFRRNYMKPNSIAVFTNDRPKSYSFAALEWLYYESKQRGVYIQHAQNEGEEKIGNYRVDGFAKEGKIIFSFQGCFWHGCLKCFNEDTMHPAKNESMGEVFKRSEKVKNIFMSMKGYQYVEIWEDEWQDLKKLFHQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BB62</accession>
    <name>A0A9Q1BB62_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Extracellular sulfatase Sulf-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_39758</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sulfatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BB62"/>
    <dbReference type="OrthoDB" id="96314at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005539">
        <property type="term" value="F:glycosaminoglycan binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008449">
        <property type="term" value="F:N-acetylglucosamine-6-sulfatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000917">
        <property type="entry name" value="Sulfatase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43108:SF16">
        <property type="entry name" value="EXTRACELLULAR SULFATASE SULF-1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43108">
        <property type="entry name" value="N-ACETYLGLUCOSAMINE-6-SULFATASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00884">
        <property type="entry name" value="Sulfatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sulfatase N-terminal" evidence="4">
        <location>
            <begin position="19"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="222"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="333"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="377"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="232"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="379"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8020225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="544" mass="63274" checksum="1175E3F1D763F397" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BB87</accession>
    <name>A0A9Q1BB87_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Cubilin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_39840</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BB87"/>
    <dbReference type="OrthoDB" id="10009301at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24251">
        <property type="entry name" value="OVOCHYMASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040346140" evidence="4">
        <location>
            <begin position="16"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="5">
        <location>
            <begin position="142"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="5">
        <location>
            <begin position="279"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="5">
        <location>
            <begin position="396"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="5">
        <location>
            <begin position="534"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="5">
        <location>
            <begin position="653"/>
            <end position="771"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8020285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="801" mass="90773" checksum="02C7BF64F4F84102" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BCD1</accession>
    <name>A0A9Q1BCD1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Potassium channel subfamily K member 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HOLleu_39856</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Nanhai2018</strain>
            <tissue evidence="16">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the two pore domain potassium channel (TC 1.A.1.8) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BCD1"/>
    <dbReference type="OrthoDB" id="297496at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015271">
        <property type="term" value="F:outward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022841">
        <property type="term" value="F:potassium ion leak channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030322">
        <property type="term" value="P:stabilization of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003280">
        <property type="entry name" value="2pore_dom_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003092">
        <property type="entry name" value="2pore_dom_K_chnl_TASK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11003:SF291">
        <property type="entry name" value="IP11374P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11003">
        <property type="entry name" value="POTASSIUM CHANNEL, SUBFAMILY K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038061">
        <property type="entry name" value="K_channel_subfamily_K_type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01333">
        <property type="entry name" value="2POREKCHANEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01095">
        <property type="entry name" value="TASKCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 13 16" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9 12 13" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 12 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 12" id="KW-0630">Potassium</keyword>
    <keyword evidence="6 12" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="4 12" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12 13" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="78"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="159"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="190"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="220"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="15">
        <location>
            <begin position="76"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="15">
        <location>
            <begin position="171"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR038061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF07885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ8020298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="354" mass="40340" checksum="448E580B7C6FAEBD" modified="2023-09-13" version="1">MKRQNVRTLLLIVCTFLYLLLGAAIFDALESKNEEREKARLEDIETTIQMRYNISDGDMEKLRDTTIELLPQKAGVQWKFAGSFFFCMTVITTIGYGHSTPRTNGGKVFCMIYSLIGIPLNLVMFQSVGERLNIFMGFGIKNLKRLMRFKKAEVSHTELVMIGGLTTGFITITGSLAFQKFEGWGFLDAYYFITITLTTIGFGDLVVLQKNNHLQKSPQYVFFSIMYILGALVVVASVMNLLVLRLLTLNTEDEKREQQEQAMRQSAIELNHIDSHIAFQSNHNTSSGPLLGSKETKNRGALYDPFTLNSSNLHANSLHLSLYRQTIYDTQYHDLDYTLEQFRNSERSGKRRSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BEY6</accession>
    <name>A0A9Q1BEY6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cytochrome c oxidase assembly protein COX18, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_37712</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Membrane</location>
            <topology evidence="1 5">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the OXA1/ALB3/YidC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BEY6"/>
    <dbReference type="OrthoDB" id="2148490at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032977">
        <property type="term" value="F:membrane insertase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032979">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane from matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20069">
        <property type="entry name" value="5TM_Oxa1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001708">
        <property type="entry name" value="YidC/ALB3/OXA1/COX18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028055">
        <property type="entry name" value="YidC/Oxa/ALB_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12428:SF65">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX18, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12428">
        <property type="entry name" value="OXA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02096">
        <property type="entry name" value="60KD_IMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Membrane insertase YidC/Oxa/ALB C-terminal" evidence="7">
        <location>
            <begin position="158"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="409"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8022729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="436" mass="49452" checksum="1636C0527137FBBC" modified="2023-09-13" version="1">MNSPSIVRLFAKEVSKGSVAHESLICRIKASRSNACGRGFTWVLPSSHMKVPVCEMHSRSLQRGLIESHHIRLASTITSRYTELHSCKFQRSHYYRNVKVLHSNGHRFLLETPVQPVVIKRFSSTGPTTVYEHILSSKPVHLAQSIFELGHNLTGLHWAGSIVATTLFLRFSLTFPLSVYAQYIRARVESLQPEILGRAKYVFAQRFGARIKNEKWSEKRAQKAFLGMVRLQAKEIYIRENCHPAKGSILLLVQLPLWIIMSLSLRNMSGAFSQRFFTDPEYLVPEFATEGALWFPDLMVPDPYMVLPILVGVLNLVNIEMHALHKGRISGFQRGLNNFLRVVSVVMIPVAAYVPSSMALYWTVSAGYGFGQNVVLKLPTVRTFVGIPKSSSDSTTPFRDMKEVFQQRYLQRGKSNKEETEPLSVLEESPRKKSEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BF87</accession>
    <name>A0A9Q1BF87_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_38094</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8023029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BF87"/>
    <dbReference type="OrthoDB" id="5974147at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8023029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="50" mass="5313" checksum="8888339421E51830" modified="2023-09-13" version="1">MSVNDEANESSQANQIFDMNVSAPQTPQPHPLTNIAPPPPIQAKEGAAEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BIV8</accession>
    <name>A0A9Q1BIV8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">LYR motif-containing protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_32547</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BIV8"/>
    <dbReference type="OrthoDB" id="74240at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20262">
        <property type="entry name" value="Complex1_LYR_LYRM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008011">
        <property type="entry name" value="Complex1_LYR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045293">
        <property type="entry name" value="Complex1_LYR_LYRM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005162">
        <property type="entry name" value="Retrotrans_gag_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33198">
        <property type="entry name" value="ANK_REP_REGION DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33198:SF19">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05347">
        <property type="entry name" value="Complex1_LYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03732">
        <property type="entry name" value="Retrotrans_gag"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="292"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="303"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="306"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="349"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8027411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="483" mass="55461" checksum="6EDE11F32D819179" modified="2023-09-13" version="1">MVKIFQDHFHVSVCIHVHCKVLYSVHGVNYHIWTQCILQCGYHVLFRYNVTITYEVITQVKRIYVRVKLGLVSHKLTTKMASIKLGAFSPAEEKWSSYCERLEQYFIVNDLTVDKKQVAVLIAAMGSKTYSVLKDLASPRKPSDIYKDIVDKLRAHFSPMPPAMGERFRFYARKQTQGESVNQYLAELKRLASTCNFDQFLNEALRDAFVFGLRDEHAQRRLFAYDDKLTLENAFSEAVSLETASAINTAMVRGPASQTSTVNALNMAQLKCFCGGKRGHVKRECRYRNAECKECGRRGHLASQCRSRERSGGEPKRSDSKKNKKKWDSKVNHMRDKEEVFTSDEGSDSDNPTSDGQSSWPVYTIKVAHETGYVNKPLMAKVKVPILLSEKPVDISQDFHPAQPFPFPDPEFLLRQQVISLYRDAFKTIRTLEDSNQRQELAVWTREEFKRNKNEDDEGAIKLLLMNGRQTLKEMKQAISMAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BJ76</accession>
    <name>A0A9Q1BJ76_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reelin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_32752</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BJ76"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002861">
        <property type="entry name" value="Reeler_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02014">
        <property type="entry name" value="Reeler"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reelin" evidence="1">
        <location>
            <begin position="3"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8027576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="186" mass="20291" checksum="14E6D9A16F3EC5D0" modified="2023-09-13" version="1">MSSSFTSVYMAAEDKASNGHLGTWSYPVTDAEYSSFECFGGDTVIAFRSNVSTTTVSFTWSLPINAKIPAQGFFFKATIVENGTAYWSEVASVTVHVEFTCFEDSILFFPSGDVGVFVIIPEPVCSRQMVSCNGTLSGTYPIGEHIIQCNCTDGNESTDTCDISFEVGKNVCKQIFDNHNLIKCKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BJC2</accession>
    <name>A0A9Q1BJC2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_29723</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8027697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BJC2"/>
    <dbReference type="OrthoDB" id="8912020at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002218">
        <property type="term" value="P:activation of innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042509">
        <property type="entry name" value="ZCCHC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22639">
        <property type="entry name" value="GAG-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22639:SF3">
        <property type="entry name" value="ZINC FINGER CCHC DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="97"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="114"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="187"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="193"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="218"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="244"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="254"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="283"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8027697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="301" mass="33891" checksum="167C9EFAEF279FF6" modified="2023-09-13" version="1">MFGVPSYITDNDLSEKLSAYGCVFRSNWTHKTYKEFPNIENGIRFVRLELPNTKVSLPYAIVVNGLHLRLKHNGQQKVCNLCLEQDHIMKNCPEYNCRECGQQGHSEARCPEVKCFKCNNLGHKSFNCPEADEGNEVEMDTVNEDKPTKYVDDVDSKAEVRDKEKTIDTRNNTKVSNEVNEVVPVMNVNDGNRNVEVRGSDKPKDTGSNMEDMIVNDKASTSRLCSQPRGSTVTPSTKRNLSCDETDDSENSQDPDASSQPCGSLSRPKKHITPNLMLARKFTPREAYETKKHGDHNSADN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BMC3</accession>
    <name>A0A9Q1BMC3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_28630</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BMC3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040280327" evidence="4">
        <location>
            <begin position="24"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="148"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="193"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="302"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="337"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="199"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="229"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="242"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="313"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="337"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="348"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8029275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="381" mass="42130" checksum="18183C6F0D987A49" modified="2023-09-13" version="1">MDSSLCQVFKGVCLAVFLSSVHGHGDCTGEKIQLRGPEGWYCDGQWRQVEILCGSQLGTISSDVKDISSRTCNQLGCDGESEASRTTKTSKKDEPVWRVNCDDWNELSLTGCHFTRAKEKESLPIINVKCSAELDDTSTLITIDVVEFALFFVTIILGSILLYHFVLWKFNSSSKENDQPKSRPTLSSSHVMVHVGTDDPNEPEAVDQGDEEHGERSALIIDSSCQEVTTEKAGHTSKHDTVEEEGTEVEETPELVYNPCYGAVTVKDEHSSKHETIEQEDTELGEIPESVDNDCYEPITEKAENSSNHDWSAEEGTESEETPELVYNVCYGAVTSNVGQSSTQDTSNEQEDTELGETTEFVDNVCYEGITEEAGRPHQTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BMT3</accession>
    <name>A0A9Q1BMT3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_28947</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BMT3"/>
    <dbReference type="OrthoDB" id="10042249at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000193">
        <property type="entry name" value="Zinc finger protein 300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051969">
        <property type="entry name" value="Zinc-finger_DNA-bd_regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45944">
        <property type="entry name" value="SCHNURRI, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45944:SF2">
        <property type="entry name" value="SCHNURRI, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="338"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="411"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="112"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="136"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="257"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="456"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="12"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="120"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="136"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="289"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="310"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8029531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="492" mass="54524" checksum="BB4FB7DF496267B1" modified="2023-09-13" version="1">MSGNGVTPGDINLEDATDDVEEGEEMQFADVDSEDTDSVCSDNSEDGDEMEEDEVEHRETSVFDKSPFGIKSMSQITRVGSCTTSMSAISDSGLSSSLMALHQRMGLRRSLSEVHLASQSEKESKEKDPAREQRLDELMGKRQKPHKSESELSKTSHDPTRLSLPGIPEEGNNAIKHLLLTRHLFGHFPWRAYQNLRTVLASFHITINVKCFPSPTLMVPFSPVSGRPPLVSPMSPSKLSQLPATVLSRVHSPSPLARNSLHFRLDPSSKAEMQPPLSDKKESTLGHIESSSAEVLQSDKSLSADDTPHTAATSSSANGEVSPEDEQALPSPSNSGPHRCSTCGKGFMKPNQLRIHCRDHTMEQYYTCFDCCLHFPTKFLLSKHERTEQHFAKVEAVEIATVGSDPEPRPFKCKECSIAFRIPGHLAKHLRSRGHLMTLERSGKLPPHKEQLLRAELGSEDKDDNYAASPESSERTDSAKKVIHLWHLFEVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BN80</accession>
    <name>A0A9Q1BN80_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Dynein intermediate chain 2, ciliary</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_29130</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynein intermediate chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BN80"/>
    <dbReference type="OrthoDB" id="10261376at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036157">
        <property type="term" value="C:outer dynein arm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045504">
        <property type="term" value="F:dynein heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045503">
        <property type="term" value="F:dynein light chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003341">
        <property type="term" value="P:cilium movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036158">
        <property type="term" value="P:outer dynein arm assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003002">
        <property type="term" value="P:regionalization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000251">
        <property type="entry name" value="Dynein axonemal intermediate chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000349">
        <property type="entry name" value="Dynein axonemal intermediate chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050687">
        <property type="entry name" value="Dynein_IC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442:SF11">
        <property type="entry name" value="DYNEIN AXONEMAL INTERMEDIATE CHAIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442">
        <property type="entry name" value="DYNEIN INTERMEDIATE CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0243">Dynein</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8" id="KW-0505">Motor protein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="543"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="155"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="251"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="158"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="197"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="251"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8029678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="711" mass="80304" checksum="F6872FF539569B9D" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BNB2</accession>
    <name>A0A9Q1BNB2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_29055</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BNB2"/>
    <dbReference type="OrthoDB" id="5988270at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8029615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="124" mass="13612" checksum="1A1509BEBFCFED22" modified="2023-09-13" version="1">MLVEEFKRCVNDDIKTHLEENKADNLKEVANLADQYALTHKFSKVGQTQNKGQFSRPNKKYGNSSGTTGNSGSQSGSISPKNSGKKQPDSSRQKKDQVSSNKAAFKDIICGFCHKKGPHNGKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BPH6</accession>
    <name>A0A9Q1BPH6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_26819</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BPH6"/>
    <dbReference type="OrthoDB" id="10035901at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47526">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47526:SF3">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="193"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8030400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="212" mass="23909" checksum="3F3BA4A9392C5453" modified="2023-09-13" version="1">MEVPIQMNEAEEDELRSKLYSESLIVDVNRIPDPFTELKDGWLCEKESKQLWPPLSYFETAAWLLVDATCPSQPSRPNVRARPNGSPSGSIGKNTNVTQFSGPLPTSLKDRLLSDYKEGKAFSYFDNKWSQELFYHHISEDSPLCFIMANCLPSQNVRAIPHMSWVCLEKETGKIRNAYCTCVQYSTEQPNVSAATHSDNSQALDRSSLHSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BQ32</accession>
    <name>A0A9Q1BQ32_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_27287</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BQ32"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8030782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="63" mass="7222" checksum="D7379CC526BE3732" modified="2023-09-13" version="1">MSKAFDNVDRGKRIKDLKVILKDNELHILAALVKDVKLRVRLGNTLVRTFTTNTVLKFPKETV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BSS5</accession>
    <name>A0A9Q1BSS5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Insulin-like growth factor-binding protein complex acid labile subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_25274</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1416801at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001164">
        <property type="entry name" value="GH01279p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000770">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.10">
        <property type="entry name" value="Tumor Necrosis Factor Receptor, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050328">
        <property type="entry name" value="Dev_Immune_Receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24373:SF384">
        <property type="entry name" value="LEUCINE RICH REPEAT CONTAINING 38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24373">
        <property type="entry name" value="SLIT RELATED LEUCINE-RICH REPEAT NEURONAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM01411">
        <property type="entry name" value="Ephrin_rec_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040423373" evidence="6">
        <location>
            <begin position="21"/>
            <end position="1308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="881"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="989"/>
            <end position="1008"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="1028"/>
            <end position="1050"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="1097"/>
            <end position="1118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="1201"/>
            <end position="1221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="1233"/>
            <end position="1253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="1259"/>
            <end position="1287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8031911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1308" mass="149961" checksum="F1525CD62C016E27" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BV44</accession>
    <name>A0A9Q1BV44_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">ATP-binding cassette sub-family A member 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_23502</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="8061355at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005319">
        <property type="term" value="F:lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03263">
        <property type="entry name" value="ABC_subfamily_A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000933">
        <property type="entry name" value="ABC transporter A family member 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000335">
        <property type="entry name" value="ATP binding cassette subfamily A member 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026082">
        <property type="entry name" value="ABCA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056264">
        <property type="entry name" value="R2_ABCA1-4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229:SF250">
        <property type="entry name" value="ABC TRANSPORTER DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER SUBFAMILY A ABCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12698">
        <property type="entry name" value="ABC2_membrane_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23321">
        <property type="entry name" value="R1_ABCA1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
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    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="2116"/>
            <end position="2145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="2166"/>
            <end position="2191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="2197"/>
            <end position="2215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="2227"/>
            <end position="2245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="2305"/>
            <end position="2326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="2835"/>
            <end position="2857"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="3088"/>
            <end position="3110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="3140"/>
            <end position="3163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="3170"/>
            <end position="3188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="3200"/>
            <end position="3220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="3240"/>
            <end position="3261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="3281"/>
            <end position="3302"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="2397"/>
            <end position="2632"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="3346"/>
            <end position="3569"/>
        </location>
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            <begin position="1829"/>
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        </location>
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            <begin position="2681"/>
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        </location>
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            <dbReference type="ARBA" id="ARBA00004141"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00008869"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8033307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="3681" mass="411970" checksum="E85A7C07C0E02777" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BWN5</accession>
    <name>A0A9Q1BWN5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_24387</name>
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    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="5949854at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8033984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="118" mass="13150" checksum="9D89A1F072A0D44E" modified="2023-09-13" version="1">MYWVNLACLRLEVGQIFVLGGGFSNAERAVMVKDGTFQDISMLASTHEEADTRLLLHTVHASGTFGRIVIWSPDTDIAVLCVHFCSNICSDVWFRSAVKDKARYIPVNQIAVRLGHKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
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    <name>A0A9Q1BXP1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
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        </lineage>
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    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8035283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="63" mass="7418" checksum="157542890AB33C1E" modified="2023-09-13" version="1">MKQLLTVQQYHPVNPNNPISCSLRAKRSRYLYRLPPQHILIYHFGLEQAELQNRKCPGKMGLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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    <name>A0A9Q1BYE3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase A2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_22091</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
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            <taxon>Metazoa</taxon>
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            <taxon>Holothuroidea</taxon>
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            <taxon>Holothuriidae</taxon>
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        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome 10"/>
    </dbReference>
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        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="retropepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001995">
        <property type="entry name" value="Peptidase_A2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13975">
        <property type="entry name" value="gag-asp_proteas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ASP_PROT_RETROV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase A2" evidence="3">
        <location>
            <begin position="12"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="222"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="222"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="234"/>
            <end position="264"/>
        </location>
    </feature>
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        <location>
            <begin position="271"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="296"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8035017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="305" mass="33814" checksum="9063432C8E96E466" modified="2023-09-13" version="1">MYVDESVCGLNLVFLVDTGANITIISPEVYSKLPEDTRPALQRVPTGLTLADGSELPFTGKATFNLTVQGHVCQHEVWVAGFAGDGIIGHDFLKNFDCHISVGEGILSIKGQRVLCEEDEAGLSSCRVERAGNTVLPPRGEVMATGVVRGNLAKQKLAMVEMSDYFMNKHNVLVARAVVDPSEGKVPVRLMYITDEPKTIYKGSTIGDCVPVEPVDRQAYNISVNNSATNHPKSNSEKSNRNEGEAKRQRQRFLEKEGRAKLDLESTSEGEEAKKLLEKLLRSSAKKEEDRLRHREAPRKHPYRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C3F1</accession>
    <name>A0A9Q1C3F1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_15973</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C3F1"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8038523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="133" mass="15189" checksum="FC826C00498D4720" modified="2023-09-13" version="1">MNLGRQNQTKEIHLCSTIPFIWLKLTFVLSQAHFTQRMAIIKGMKTALFETNSGEVFMFGNTSRIQLLSFERLYNETKNESILNTNKCTGRLVLNRVEVFTQSAVYRFLNTVRGLPCNVRPCLLSSLLSKPGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C3M0</accession>
    <name>A0A9Q1C3M0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_18395</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C3M0"/>
    <dbReference type="OrthoDB" id="6282181at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="62"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="34"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8037554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="96" mass="11291" checksum="3F77AC00E1DDDDD1" modified="2023-09-13" version="1">MEFYGAEECNNFKDLKSELQLWLRNEMDQAVTKLKGTGSKEATEDQSHKGNQKSISKEKVTCWNCGEKGHYSNECTQRRIQMYFPLENPPRRARMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C3S0</accession>
    <name>A0A9Q1C3S0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_19096</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8038111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C3S0"/>
    <dbReference type="OrthoDB" id="6118021at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45823">
        <property type="entry name" value="T-SNARE COILED-COIL HOMOLOGY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45823:SF1">
        <property type="entry name" value="T-SNARE COILED-COIL HOMOLOGY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8038111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="250" mass="28313" checksum="1EC64FC010C4D82A" modified="2023-09-13" version="1">MESGGARRIPERELSQSTHTLEILSMDPQICRICSLDGVQPWDSYISHFESFSDINSWSQCEKAQYLKASLKGDALEIFAELPNTRLDFETLSTALETRFWHAHEGPLFLAQLTMRTREKGESVQQSAQAIRKFVKGANLSLNSAAREDIAFEHFRNALGNIKLQRTVFMSKPRSLAEAVAVAAKTESFQRAQQTHDLKQRGNVRQIVEDAAENKSKSPSMANILQMLQEMKITLVELKDWQQSVKRGQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C4L5</accession>
    <name>A0A9Q1C4L5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cytochrome c oxidase assembly protein COX19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_19022</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the COX19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8038049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C4L5"/>
    <dbReference type="OrthoDB" id="268594at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051383">
        <property type="entry name" value="COX19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009069">
        <property type="entry name" value="Cys_alpha_HP_mot_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21107">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21107:SF2">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47072">
        <property type="entry name" value="Cysteine alpha-hairpin motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51808">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00039385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8038049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="130" mass="15388" checksum="8E41C04798B32D4A" modified="2023-09-13" version="1">MSTAMNFGQKSFKPRPPDKGSFPLDHEGECRKFKEIFMECIRANRYQSNKCRLESKQYLQCRMDRDLMAKESFDKLGFHDTDDKVKTDVKGDSDELDTGQIDISKYIFLLQIEAQYGELQVFPYFYRLKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C4R4</accession>
    <name>A0A9Q1C4R4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Sushi domain-containing protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_04924</name>
        <name evidence="7" type="ORF">HOLleu_16896</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C4R4"/>
    <dbReference type="OrthoDB" id="5971801at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005533">
        <property type="entry name" value="AMOP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051495">
        <property type="entry name" value="Epithelial_Barrier/Signaling"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13802">
        <property type="entry name" value="MUCIN 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13802:SF52">
        <property type="entry name" value="MUCIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03782">
        <property type="entry name" value="AMOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00723">
        <property type="entry name" value="AMOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50856">
        <property type="entry name" value="AMOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040654157" evidence="5">
        <location>
            <begin position="17"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="AMOP" evidence="6">
        <location>
            <begin position="31"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8039243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8046299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="178" mass="19840" checksum="45F8AA2D2A8B1E88" modified="2023-09-13" version="1">MKMKLVLLAIITCVVASLSDYLVSASSKLTLSIMGKSNCSDWMSVLRLVYITELPPCPCTYSQAINDDKFILSNFLIDYYHNGAANCFRAPSQTSLSESGQQCCYGDDGNILIGIEQNGGTADAYSPDGVKNFGRHIWYDVLPWVACCELGNRETCEIYYQFRPSDDCLEYRGPVYTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C4T2</accession>
    <name>A0A9Q1C4T2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Calcium channel flower</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_16191</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the calcium channel flower family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C4T2"/>
    <dbReference type="OrthoDB" id="9934994at2759"/>
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        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019365">
        <property type="entry name" value="TVP18/Ca-channel_flower"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13314">
        <property type="entry name" value="CALCIUM CHANNEL FLOWER HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13314:SF2">
        <property type="entry name" value="CALCIUM CHANNEL FLOWER HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10233">
        <property type="entry name" value="Cg6151-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01077">
        <property type="entry name" value="Cg6151-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="33"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="61"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="103"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8038696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="187" mass="20063" checksum="71AC3C061D98B53C" modified="2023-09-13" version="1">MSSFGYSKQTLQGDLPEDPNNAPAQPQQQQAGFFVRTAIHIIGAIAGGLAMFLGLFSCFSLSGLCILSGLYLMLLGFGLIVLEAPICCQFWEITRKLGEWSNSMSALLKAGLYLLLPIASFFMCRGISQIFACLLVMGVGALYGIIFIGKKGDAVKIARQQHVTGTDGSQAVTMSEVKPQADVGRYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C5E8</accession>
    <name>A0A9Q1C5E8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_17379</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C5E8"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040312452" description="Ig-like domain-containing protein" evidence="3">
        <location>
            <begin position="24"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="379"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="440"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8039603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="459" mass="51970" checksum="71D18EE663B5E58C" modified="2023-09-13" version="1">MKEFRTLGSWLLFFLVILHETQQYDSHLGVVVNIDVDGWDVVKTGIREYSFADSHFVTIKCSAVISSAANISLLDSHGQPIISNVSSRNEITVVTKRLRQPVGKYYCLLNIPIWSLNDSYSELVISSEVDIIQESVLPLYASSVGNSRYVGERAFLYFTKVGRISPSCSVEKSSENEREFFIDGNFPEEKCVCYYYSRYKKCSVLRLSVYPFLKLNIYEENVTLDSSVMEFSCNSTPPRLMYWKMFGSHGNLFDFDNIDHSISVNITANITQSPGKTTLRIWETVPGGNGIHTVMCSTYDTDKRVVAFLPVTTPSVCNSDCASSDLKQTDMPESTTTTTHKSGTFVMTTTDLEGHYDDTEVQASTEQECEMKTYTLNGYVLTTYVSAPYLVIILLLFGIICYMHHKNRRSLKMEFTTELKVDHTLPETVLHDNPAYLSFSETNGYSPENENDEKTYANL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C6G4</accession>
    <name>A0A9Q1C6G4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Regulator of G-protein signaling 8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_17344</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
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            <taxon>Metazoa</taxon>
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            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="CDD" id="cd08713">
        <property type="entry name" value="RGS_RGS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.167.10:FF:000001">
        <property type="entry name" value="Putative regulator of g-protein signaling 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016137">
        <property type="entry name" value="RGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034951">
        <property type="entry name" value="RGS_RGS3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10845">
        <property type="entry name" value="REGULATOR OF G PROTEIN SIGNALING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10845:SF259">
        <property type="entry name" value="RGS DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00615">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01301">
        <property type="entry name" value="RGSPROTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50132">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="2">
        <location>
            <begin position="62"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="RGS" evidence="3">
        <location>
            <begin position="879"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="180"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="236"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="284"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="612"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="659"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="754"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="844"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="182"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="242"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="620"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="665"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="721"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="768"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="786"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="856"/>
            <end position="866"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8039581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1001" mass="113258" checksum="C3C6A3DCEB6ACB1A" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C730</accession>
    <name>A0A9Q1C730_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DNA repair endonuclease XPF</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_14853</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the XPF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C730"/>
    <dbReference type="OrthoDB" id="361020at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000110">
        <property type="term" value="C:nucleotide-excision repair factor 1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000014">
        <property type="term" value="F:single-stranded DNA endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901255">
        <property type="term" value="P:nucleotide-excision repair involved in interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000712">
        <property type="term" value="P:resolution of meiotic recombination intermediates"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20078">
        <property type="entry name" value="XPF_nuclease_XPF_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10130:FF:000002">
        <property type="entry name" value="DNA repair endonuclease XPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006166">
        <property type="entry name" value="ERCC4_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006167">
        <property type="entry name" value="XPF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047520">
        <property type="entry name" value="XPF_nuclease"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00596">
        <property type="entry name" value="rad1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10150">
        <property type="entry name" value="DNA REPAIR ENDONUCLEASE XPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10150:SF0">
        <property type="entry name" value="DNA REPAIR ENDONUCLEASE XPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02732">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00891">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="RuvA domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 13" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ERCC4" evidence="12">
        <location>
            <begin position="665"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="336"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="423"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="620"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="889"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="341"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="443"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="483"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="620"/>
            <end position="632"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00072370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8040533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="913" mass="103830" checksum="86C776BC1BC835FC" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C781</accession>
    <name>A0A9Q1C781_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Hexosyltransferase</fullName>
            <ecNumber evidence="11">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_16764</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Golgi apparatus membrane</location>
            <topology evidence="1 11">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the glycosyltransferase 31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="11"/>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C781"/>
    <dbReference type="OrthoDB" id="2139606at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.50:FF:000001">
        <property type="entry name" value="Hexosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002659">
        <property type="entry name" value="Glyco_trans_31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214">
        <property type="entry name" value="BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214:SF314">
        <property type="entry name" value="HEXOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01762">
        <property type="entry name" value="Galactosyl_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8039141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="299" mass="35153" checksum="4A3334E19E590586" modified="2023-09-13" version="1">MFFKVQDTNIHTHLTSSPDNIEAMKSQAVTFKIRDLCNVTRKNTKEVFLLVLMLSAPMNFEQRQNQRQHCLNVSEVSGKRIERLFLLGNSADKFLNKKIMEEFKIFQDILIGDFQDSYKNLTLKTLSGLKWASLYCSQARYVMKVDDDTLINFKTLIDTLTISPKEDFITGHVCRRCRPVRDQMHKWYISLDEYPKSFYPNYVKGLAYVMSGDFPAKIINMSHKTPFFLWEDVYIGMILEKLAIRPHNNRCFTMKPHLDEFSLCKYITACAIHFNNRPQDDFRYCKSLKQFHENATDNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C819</accession>
    <name>A0A9Q1C819_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Dynein light chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_15493</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Cytoplasm</location>
            <location evidence="2 10">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the dynein light chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8041013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C819"/>
    <dbReference type="OrthoDB" id="10033309at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005868">
        <property type="term" value="C:cytoplasmic dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.740.10:FF:000005">
        <property type="entry name" value="Dynein light chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.740.10">
        <property type="entry name" value="Protein Inhibitor Of Neuronal Nitric Oxide Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037177">
        <property type="entry name" value="DLC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001372">
        <property type="entry name" value="Dynein_light_chain_typ-1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11886">
        <property type="entry name" value="DYNEIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11886:SF35">
        <property type="entry name" value="DYNEIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01221">
        <property type="entry name" value="Dynein_light"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01375">
        <property type="entry name" value="Dynein_light"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54648">
        <property type="entry name" value="DLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-0243">Dynein</keyword>
    <keyword evidence="5 10" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8041013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="123" mass="14118" checksum="91F3570A5CDEEF19" modified="2023-09-13" version="1">MTSQICGLTRRLPPSVPKVMRHFLDYRVGKDEGNSSVGGITVEHTDMENEMKQEAIAVTEDAFEQFTVEEEIENFIKNEFEEKYGNTWTCIVGRKKGSYVTHETNEFIFMRAGQVDVLLYRSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CDC0</accession>
    <name>A0A9Q1CDC0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_13498</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8042439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CDC0"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="68"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8042439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="90" mass="10028" checksum="A4C072C8678918D0" modified="2023-09-13" version="1">MPRFEQTGKAKNKAVDCTICVLSPPKLSKFSKREKTPPRDGDHKSPRTENRPVENPENMIISCQIGKMYINFGSSMICISVGLWAACDVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CDL8</accession>
    <name>A0A9Q1CDL8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_10033</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CDL8"/>
    <dbReference type="OrthoDB" id="6022531at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008528">
        <property type="term" value="F:G protein-coupled peptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009755">
        <property type="term" value="P:hormone-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24372:SF77">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24372">
        <property type="entry name" value="GLYCOPROTEIN HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="53"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="95"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="141"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="180"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="262"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="308"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="340"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="9">
        <location>
            <begin position="65"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8043087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="377" mass="42386" checksum="41EE64CD5370A30A" modified="2023-09-13" version="1">MEKLTFIPISLFFHRRIDNYGICCLFKESVDCRTPPSPFKTCDSMLPNNLLRVALWVICLISLLTNMVVIVGRIKYKIPAMRNMFSSTVSDKQNALLINLAVADFLMGVYLLAIGIADAVFGKAFFLSALNWRNGIVCKGIGLVGFVANIASILTLTFVSVERFFTIVFPFGKYTFPPKLIATICVTIWVTCGLMALTPIILSESVKQIFGFSDICMGLPFVSLPDTSKYYDTFDEHFWYNQLEMNEITDVKDFQWVFSQIVYIYFSATCVTVITLCYIAIFISAIVTKIKSGRPGGNKDEIKMALRISVIVGTDLLCWLPVIITGILSQTGLSMSTDVYVWFAIFVMPINSALNPFIYTIPTIKRRKKNEPSFIAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CEU8</accession>
    <name>A0A9Q1CEU8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_10513</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CEU8"/>
    <dbReference type="OrthoDB" id="10063284at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052958">
        <property type="entry name" value="IFN-induced_PKR_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46289">
        <property type="entry name" value="52 KDA REPRESSOR OF THE INHIBITOR OF THE PROTEIN KINASE-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46289:SF17">
        <property type="entry name" value="HAT C-TERMINAL DIMERISATION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8043433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="172" mass="19620" checksum="821D469F846E184A" modified="2023-09-13" version="1">MPVETAEAVTFFGTIQKVYTFFTSSQPRLNRLEQAQENLGMEKTKLQRLCETRWYCRHDSVKAIKVLYPALLQAIEDITENGTFPETKAEARGLLEFMSTFEFVFMIGMWSKVLYEMSTLSEYMQQVSMDLVTASSLIGAAMKNLEQQRSNEVFNGILEEARAIATREGVTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CFS4</accession>
    <name>A0A9Q1CFS4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_11128</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CFS4"/>
    <dbReference type="OrthoDB" id="10066781at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33480">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33480:SF1">
        <property type="entry name" value="TYR RECOMBINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8043850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="325" mass="36833" checksum="C30A37EA1387742B" modified="2023-09-13" version="1">MNITAGFLKKKSGQLIVLRRPQEDEESTAEMYGPCPFCLGFLKMSELWRQKKSSQFAKDKTDSSSLRKMSKMMLAESIAEPGVSSNLHKYVFSSMKRDQESLISKNDPLICKFGMDHLDTKSVRSAHVVSQQMRLLSRLLLEIRKLPPCCPAPNKDLAHILNPSHFDVLVEGIKKLCKYQAGNLHDDCPGSFSTPSVVLKLGPYLKECAMLQRGKALREGDVDVVSSVDRFLQLHVSEYKKLSSIAVKQKDTAKFNKQDMLPLTSDIKKLRDYQVAEIERLSKLVNEDNISSYRQLCNVFLSRVICFNKRRSGEASLMKIASYKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CGY6</accession>
    <name>A0A9Q1CGY6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_11730</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8044299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CGY6"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="305"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="338"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8044299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="456" mass="50654" checksum="65C904A6DE9C0853" modified="2023-09-13" version="1">MYRTINEGLPARFTLFLEVNLSSLKSMKFVNLFGPFKRFHVLILLFSSFVVSLEGQCMDHGIRNTAADDEDDDNSGGGDDGDVDDDDEDAHRNWQLYNFNGSCQICFLRNGRPHDAELILPCVDQNQQIVDTINISYAFQRHGTGRSSLSGEPLTYYNLSNDIECGMNTSPGTQLKTVEMNPFTDSRNAHVHGHIEIKRQRENSYRLKVSNSVQVSVVCLKEVELLSAVTSARPTPSPTTASTRVTTGSMPIGTGISTAALTNIGIVTIKTHEVGLADHTTTTTSGRSSSQGNQRTAIEQGQSYFPIWVIFVIIAIVIISICAVAGVFIRRRKRHDHQPANNVNTGRVNDESNGIKGIMKNDKDVIPNPTYGQEHFNEKEEEYHSYTDIDVKVPANSEMIYNSAYESPPASSDMLYNSAYESEEANNDMVYNSVYESKETKSDMIYNSAYESSTSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CI74</accession>
    <name>A0A9Q1CI74_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Distal membrane-arm assembly complex protein 1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_08138</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CI74"/>
    <dbReference type="OrthoDB" id="10059044at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028036">
        <property type="entry name" value="DMAC1-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15055">
        <property type="entry name" value="DMAC1_Dmo2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="Distal membrane-arm assembly complex protein 1-like" evidence="2">
        <location>
            <begin position="28"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF15055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8045188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="118" mass="12444" checksum="8FCE2E18B3ED7F43" modified="2023-09-13" version="1">MGSEQPGSWIQNQKEKVKEDVTDFRLKDCAGCRLIGGIGIILAGGYVGFVGQKRAKLRGGGIISQIPSAIGAGVMISLGIMRLLGINPFTSSDSEIGIDALQKLRRGNEDVTSESTPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CI75</accession>
    <name>A0A9Q1CI75_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Actin-related protein 10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_07997</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the actin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CI75"/>
    <dbReference type="OrthoDB" id="337660at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="CDD" id="cd10207">
        <property type="entry name" value="ASKHA_NBD_Arp10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8045069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="460" mass="50795" checksum="14033B448E3D7FF3" modified="2023-09-13" version="1">MEALNVVVNVAFVLEKQSFINLRLSAVPDSDPFTMPLFEGLSAGSEKSAVILDIGAAYSKVGFAGEFGPWAFVSSKVKLSKTKKEVFVWDYSSEEELFDILVEFFHLLYFKHLLVNPKDRRVVFCESILCPTQFRETVAKVLFKHYEVPSILFAPSHLLAMFTLGINTGLVLDAGFSETTVLPIYEGIPIIKGVQTVTVAAREIHRNLAALLQDQAVLITDGEEAPLASKMSSLPDTVIEDIKVRTCFVTNINRARLIQQSILQNDESKWPAVPSSVEYPLDGGSKLVVTGKVREVACEVLFEQDMEEKSVATTILDAILQCPLDMRIPLAQNLVLMGGTSMLPGFAHRLVDELKELLSKPQYKDRLAISSFKVHSPPSEANYTAWLGGSLFGAMEILSYRSLSREAYNQAGKVPDWCSLDDAVQEAEVVPKERSSMKQLEALRKLSLEKSSAGASPTPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CML4</accession>
    <name>A0A9Q1CML4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">F-box only protein 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_06108</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CML4"/>
    <dbReference type="OrthoDB" id="101791at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903599">
        <property type="term" value="P:positive regulation of autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22087">
        <property type="entry name" value="F-box_FBXO7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1000.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047118">
        <property type="entry name" value="Fbxo7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15537">
        <property type="entry name" value="F-BOX ONLY PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15537:SF2">
        <property type="entry name" value="F-BOX ONLY PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50804">
        <property type="entry name" value="SCAN_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCAN box" evidence="3">
        <location>
            <begin position="105"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="453"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="221"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="584"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="597"/>
            <end position="607"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8047179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="646" mass="72861" checksum="37E80F402029301D" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CMM9</accession>
    <name>A0A9Q1CMM9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Patched domain-containing protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_06785</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the patched family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CMM9"/>
    <dbReference type="OrthoDB" id="6510177at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1640.10">
        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051697">
        <property type="entry name" value="Patched_domain-protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003392">
        <property type="entry name" value="PTHD_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000731">
        <property type="entry name" value="SSD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10796">
        <property type="entry name" value="PATCHED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10796:SF92">
        <property type="entry name" value="PATCHED-RELATED, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02460">
        <property type="entry name" value="Patched"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82866">
        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50156">
        <property type="entry name" value="SSD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="21"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="264"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="295"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="329"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="368"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="400"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="483"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="721"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="749"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="793"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="823"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="domain" description="SSD" evidence="8">
        <location>
            <begin position="264"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="domain" description="SSD" evidence="8">
        <location>
            <begin position="687"/>
            <end position="845"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8047721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="871" mass="96833" checksum="36DC4667EF08DFF7" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CRX3</accession>
    <name>A0A9Q1CRX3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Calcium-responsive transcription factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_04347</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CRX3"/>
    <dbReference type="OrthoDB" id="5984937at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029309">
        <property type="entry name" value="CaRF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048324">
        <property type="entry name" value="ZSWIM1-3_RNaseH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47456">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47456:SF1">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15299">
        <property type="entry name" value="ALS2CR8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21056">
        <property type="entry name" value="ZSWIM1-3_RNaseH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="3">
        <location>
            <begin position="630"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="780"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="785"/>
            <end position="806"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8050957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="854" mass="98780" checksum="6D8656CECA1E0077" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CST3</accession>
    <name>A0A9Q1CST3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_03625</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CST3"/>
    <dbReference type="OrthoDB" id="10060997at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47510:SF3">
        <property type="entry name" value="ENDO_EXONUCLEASE_PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47510">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8050421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="120" mass="13598" checksum="81E9734EFD6D8F69" modified="2023-09-13" version="1">MKAPTKSCELDPIPTSLIKHCIKDIVPFLTCVINKSLGSGVFPTDLKLAYIRPLLKKPSLDKEDFKNFRPVANLPFLGKLIERIVSPRLWSVITQDDLQDPFQSAYRPHHSTETFTTCSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CU41</accession>
    <name>A0A9Q1CU41_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_04541</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8051098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CU41"/>
    <dbReference type="OrthoDB" id="2525164at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017868">
        <property type="entry name" value="Filamin/ABP280_repeat-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50194">
        <property type="entry name" value="FILAMIN_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="Filamin" evidence="1">
        <location>
            <begin position="4"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8051098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="522" mass="58841" checksum="A1EC2097A636F190" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CUE9</accession>
    <name>A0A9Q1CUE9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_04437</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8051024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CUE9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8051024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="70" mass="8130" checksum="3FB02EF19E3167CB" modified="2023-09-13" version="1">MVKLCKESWNAKIKTEMSYSSDSANSQINTEGIRFVAHIRQSEMLNELVNLKDILTNKEVYASKLEFTRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1GXS3</accession>
    <name>A0A9Q1GXS3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Protocadherin-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HOLleu_31117</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Nanhai2018</strain>
            <tissue evidence="17">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6252479at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000020">
        <property type="entry name" value="Dachsous cadherin-related 1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000004">
        <property type="entry name" value="Protocadherin 1 gamma 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000092">
        <property type="entry name" value="Protocadherin 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000002">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000007">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000123">
        <property type="entry name" value="Protocadherin beta 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013164">
        <property type="entry name" value="Cadherin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050174">
        <property type="entry name" value="Protocadherin/Cadherin-CA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028:SF146">
        <property type="entry name" value="CADHERIN 96CB, ISOFORM D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028">
        <property type="entry name" value="CADHERIN-87A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08266">
        <property type="entry name" value="Cadherin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 12" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 15" id="KW-0732">Signal</keyword>
    <keyword evidence="3 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040247841" evidence="15">
        <location>
            <begin position="28"/>
            <end position="1282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="809"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="25"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="137"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="249"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="362"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="468"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="571"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="683"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="896"/>
            <end position="1102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1125"/>
            <end position="1282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="912"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="925"/>
            <end position="947"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="948"/>
            <end position="964"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="971"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1000"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1027"/>
            <end position="1038"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1060"/>
            <end position="1070"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="1076"/>
            <end position="1090"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1091"/>
            <end position="1102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1129"/>
            <end position="1142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1144"/>
            <end position="1157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1167"/>
            <end position="1176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="1177"/>
            <end position="1188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="1194"/>
            <end position="1206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ8028782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1282" mass="142035" checksum="A77A0FFFCEF5BAB6" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1GYU4</accession>
    <name>A0A9Q1GYU4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Ufm1-specific protease 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_30448</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C78 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1GYU4"/>
    <dbReference type="OrthoDB" id="417506at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071567">
        <property type="term" value="F:deUFMylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.130:FF:000001">
        <property type="entry name" value="Probable Ufm1-specific protease 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012462">
        <property type="entry name" value="UFSP1/2_DUB_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049387">
        <property type="entry name" value="UFSP2-like_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48153">
        <property type="entry name" value="UFM1-SPECIFIC PROTEASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48153:SF2">
        <property type="entry name" value="UFM1-SPECIFIC PROTEASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07910">
        <property type="entry name" value="Peptidase_C78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20908">
        <property type="entry name" value="UfSP2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 8" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UFSP2 second" evidence="7">
        <location>
            <begin position="187"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="domain" description="UFSP1/2/DUB catalytic" evidence="6">
        <location>
            <begin position="431"/>
            <end position="615"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8028259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="623" mass="70136" checksum="88ADBE92AE2F9A77" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H084</accession>
    <name>A0A9Q1H084_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_30233</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H084"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF13">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="274"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="78"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="288"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8028095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="309" mass="36323" checksum="86EBE37D070F98AB" modified="2023-09-13" version="1">MPFSLDKFVASPSVEELDSLKKSEIVKVAKHYGIEFQPLMRKDEIKRYVLEYPVDESILPSTVLETAITVPTDNTFELKRLEIEMSKEVRLKEMEREERERREAREHEFRLKQLELGVVKGSDPKIGLDTGGFDVSKHVKFVPKFQEDNVEKFFNHFEKLGEQLKWPRDKWSILIQSNFTGKAQEVYSALSIEDSMDYDKVKKAILQAYELVPEAYRQKFRKYRKADTQTYVEFAYQKERHFDRWCASKKVSTFDTLRQLMLVEEFKRRPKLFQGGGGSVKRRPVRNTGLNPSTSEWKVPENSTSTQPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H0G0</accession>
    <name>A0A9Q1H0G0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Maltase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HOLleu_31084</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Nanhai2018</strain>
            <tissue evidence="18">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H0G0"/>
    <dbReference type="OrthoDB" id="1334205at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045177">
        <property type="term" value="C:apical part of cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004558">
        <property type="term" value="F:alpha-1,4-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06602">
        <property type="entry name" value="GH31_MGAM_SI_GAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14752">
        <property type="entry name" value="GH31_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00111">
        <property type="entry name" value="Trefoil"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1180:FF:000001">
        <property type="entry name" value="Maltase-glucoamylase, intestinal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1180:FF:000005">
        <property type="entry name" value="Maltase-glucoamylase, intestinal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1760:FF:000001">
        <property type="entry name" value="Maltase-glucoamylase, intestinal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000016">
        <property type="entry name" value="Maltase-glucoamylase, intestinal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1760">
        <property type="entry name" value="glycosyl hydrolase (family 31)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.110.10">
        <property type="entry name" value="Spasmolytic Protein, domain 1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030458">
        <property type="entry name" value="Glyco_hydro_31_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048395">
        <property type="entry name" value="Glyco_hydro_31_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025887">
        <property type="entry name" value="Glyco_hydro_31_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000322">
        <property type="entry name" value="Glyco_hydro_31_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000519">
        <property type="entry name" value="P_trefoil_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044913">
        <property type="entry name" value="P_trefoil_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22762">
        <property type="entry name" value="ALPHA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22762:SF133">
        <property type="entry name" value="P-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13802">
        <property type="entry name" value="Gal_mutarotas_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01055">
        <property type="entry name" value="Glyco_hydro_31_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21365">
        <property type="entry name" value="Glyco_hydro_31_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00088">
        <property type="entry name" value="Trefoil"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00018">
        <property type="entry name" value="PD"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57492">
        <property type="entry name" value="Trefoil"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00129">
        <property type="entry name" value="GLYCOSYL_HYDROL_F31_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51448">
        <property type="entry name" value="P_TREFOIL_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="16" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0765">Sulfation</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040383548" description="Maltase" evidence="16">
        <location>
            <begin position="18"/>
            <end position="1112"/>
        </location>
    </feature>
    <feature type="domain" description="P-type" evidence="17">
        <location>
            <begin position="21"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="P-type" evidence="17">
        <location>
            <begin position="74"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="P-type" evidence="17">
        <location>
            <begin position="153"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="P-type" evidence="17">
        <location>
            <begin position="209"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="P-type" evidence="17">
        <location>
            <begin position="255"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="133"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00041343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ8028753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1112" mass="123743" checksum="F4EFFFF24E3E47C7" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H2P5</accession>
    <name>A0A9Q1H2P5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">FAST kinase domain-containing protein 1, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_26690</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H2P5"/>
    <dbReference type="OrthoDB" id="385235at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035770">
        <property type="term" value="C:ribonucleoprotein granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000963">
        <property type="term" value="P:mitochondrial RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044528">
        <property type="term" value="P:regulation of mitochondrial mRNA stability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013579">
        <property type="entry name" value="FAST_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050870">
        <property type="entry name" value="FAST_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013584">
        <property type="entry name" value="RAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21228">
        <property type="entry name" value="FAST LEU-RICH DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21228:SF72">
        <property type="entry name" value="LD32258P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08368">
        <property type="entry name" value="FAST_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08373">
        <property type="entry name" value="RAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00952">
        <property type="entry name" value="RAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51286">
        <property type="entry name" value="RAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RAP" evidence="2">
        <location>
            <begin position="881"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="634"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="634"/>
            <end position="643"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8030315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="960" mass="110901" checksum="555C0E97FBD417D6" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H2V3</accession>
    <name>A0A9Q1H2V3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_26938</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H2V3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040495174" evidence="1">
        <location>
            <begin position="24"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8030495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="216" mass="24387" checksum="59CF30C1D9D3CBD2" modified="2023-09-13" version="1">MISLTFISLSILDIRLYLPASPAFPEILDDTQHVVFIAIGYSNFHDFEKLSDNCISADGTIFDLSIPLLSNGPEGIPQYSLALATFYGRERGDIPKSNIIFPYILVDEITMNETSVIMRNGTDEMQLSLFKPSGSCSIPDSITRHEFESYIKTELQFGLHEPEFFSFCDDIPLTTSIARLPKQSNAICIGCILMYVKHHGQREHLILRYVRRPSTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H3J5</accession>
    <name>A0A9Q1H3J5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Mitochondrial import inner membrane translocase subunit TIM14</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_27873</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the TIM14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8031215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H3J5"/>
    <dbReference type="OrthoDB" id="240298at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001405">
        <property type="term" value="C:PAM complex, Tim23 associated import motor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000001">
        <property type="entry name" value="Import inner membrane translocase subunit tim14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12763">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12763:SF28">
        <property type="entry name" value="GEO10507P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="J" evidence="8">
        <location>
            <begin position="57"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8031215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="111" mass="11940" checksum="C5911CEBCB20661C" modified="2023-09-13" version="1">MASTVIVAGLGIAAAAYLGRVALQAGRIVEQNAHKIPKPSFSSYYKGGFEPKMTKREASLILGVSPAASRSKVRDAHRRIMILNHPDKGGSPYMAAKINEAKEYLEGTARK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H596</accession>
    <name>A0A9Q1H596_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cornifelin-like A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_19846</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cornifelin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8035997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H596"/>
    <dbReference type="OrthoDB" id="1045822at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006461">
        <property type="entry name" value="PLAC_motif_containing"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01571">
        <property type="entry name" value="A_thal_Cys_rich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15907">
        <property type="entry name" value="DUF614 FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04749">
        <property type="entry name" value="PLAC8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8035997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="136" mass="14603" checksum="8171A43F75B203D3" modified="2023-09-13" version="1">MEPYVASGDKTVIMQQPAAGGTTIINMNAPNPMMPRGIPRDWTTGLCAMCDDMAVCLCETFVPCHVCMVASDMGEGVCMPCCIPAPWLVMRAHARGKHNIQGSLLNDCCVSLWCGPCAACQLAREIKMIKNGQAQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H6J0</accession>
    <name>A0A9Q1H6J0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Cell adhesion molecule 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_21023</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H6J0"/>
    <dbReference type="OrthoDB" id="5359219at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013162">
        <property type="entry name" value="CD80_C2-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45889">
        <property type="entry name" value="IG-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45889:SF8">
        <property type="entry name" value="IG-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08205">
        <property type="entry name" value="C2-set_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="18"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8034261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="245" mass="27569" checksum="C4F57724B89FC67E" modified="2023-09-13" version="1">MKHYQSLLSDTWTFLTYLVLICVHTEQHLLVKWERSEEGGDVVIAAYRFSQRTTQYSGDDDRYTLTFDQNKDTSIATLVISNVALDDIGDYKCELFGVSTSSATVRLEVLVPPEEVAIIDSRTNTEIFDGGILRVTSGQPTLITCLTHFSGTRPATELVWRSQDNMVETSSMSSVVESPAEPRLEQTTSKMNFTVDVTAHGKWVECQANHDGLQQPLRTRLTLDVDGMVYIYIVVEQISILESKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H6P5</accession>
    <name>A0A9Q1H6P5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">exodeoxyribonuclease III</fullName>
            <ecNumber evidence="3">3.1.11.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_22332</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.</text>
            <dbReference type="EC" id="3.1.11.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="7">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="7">Probably binds two magnesium or manganese ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA repair enzymes AP/ExoA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.11.2" evidence="3"/>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H6P5"/>
    <dbReference type="OrthoDB" id="416119at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003906">
        <property type="term" value="F:DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008311">
        <property type="term" value="F:double-stranded DNA 3'-5' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09076">
        <property type="entry name" value="L1-EN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004808">
        <property type="entry name" value="AP_endonuc_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748">
        <property type="entry name" value="AP ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748:SF26">
        <property type="entry name" value="ENDONUCLEASE_EXONUCLEASE_PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0464">Manganese</keyword>
    <keyword evidence="4 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="9">
        <location>
            <begin position="8"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="8">
        <location>
            <position position="144"/>
        </location>
    </feature>
    <feature type="site" description="Important for catalytic activity" evidence="8">
        <location>
            <position position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR604808-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR604808-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8035189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="229" mass="26654" checksum="5C16CA1CFB61D416" modified="2023-09-13" version="1">MSSDLKFISFNARGLRQAKKRRKLFAYFHRQNTDLVFLQESHSSLADAKYWTNEWGGRILFSHGSTNSRGVCILFNPQLSYKIIKEELDPFGRYIFVDIEVQNRFLTLACIYAPNIDSSTFFVDLSNFLDRFHGETIILAGDLNFVFNLEMDKTGGNPRTNFKAREKCLNLMAAYELLDIWRERNPLSKLFTWSSNVTTGIHCRLDYFLVSRSLEHSVKDVLFFTWSAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H8C2</accession>
    <name>A0A9Q1H8C2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Arylamine N-acetyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_19977</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H8C2"/>
    <dbReference type="OrthoDB" id="10260017at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2140.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053710">
        <property type="entry name" value="Arylamine_NAT_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8036106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="369" mass="42364" checksum="42E3F6DBFA79DC01" modified="2023-09-13" version="1">MNILTCSFKGFLNVSYRAFPHMHACASPLAAMRSFLTRNTEVVTSRKVIGVTFDTFAGPVRQPYPLSKEEAFSFVENVLGLEHPDTLFKTDPLKNLTKIAEGIKKVSPYTTMSFWDGSLYQLNDYYFKHRMIHGHGGGCVVINPFTKAILDHLGYTTYDVSARVVASSDAFHLSTIVCDLSYPGSIHLVEPGTIRPLFHPIALDFEQESSVYEVYGLRNKFFKRNDGNVVWCMEPLPKMLNRLGNHEVIQDSAGTKWQIFLIYMLQRKMGRCHFNEYWHKVIRGKRYIPPRTNGFVYVAYKNGDLIHVFAGKQKIFVQTLHKDATEEAQELTKEELLRLFEKNFPGFPTRMVGRAIQDNFQISQLLRNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H8C8</accession>
    <name>A0A9Q1H8C8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Receptor ligand binding region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_20251</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H8C8"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017046">
        <property type="term" value="F:peptide hormone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052612">
        <property type="entry name" value="ANP_Clearance_Receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44755">
        <property type="entry name" value="NATRIURETIC PEPTIDE RECEPTOR 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44755:SF12">
        <property type="entry name" value="RECEPTOR LIGAND BINDING REGION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040210277" description="Receptor ligand binding region domain-containing protein" evidence="5">
        <location>
            <begin position="17"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="Receptor ligand binding region" evidence="6">
        <location>
            <begin position="59"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8036315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="219" mass="24619" checksum="ECC12E8598EB6626" modified="2023-09-13" version="1">MFLAALFSCFVAITHTHTAIRIGLVLGSFGSRLDQTQLKPPFDIALKSVSERVEKGEFNNFTFTTYLRETAQNGQKIGSGLVADLVNEHDIHVVFGHPSSSEMVGIGDLVAHWNIPALTTAAINAELEDRRRFTTFTRTSMRAEAIGWLITRLFSTFEWNLCILIYSHYGPFRSISNAVLQTLQEGGVVASFLYIDNIPQNATEWLLHSKERSRSKWQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H8I2</accession>
    <name>A0A9Q1H8I2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Sulphur transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_17705</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H8I2"/>
    <dbReference type="OrthoDB" id="10254418at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007272">
        <property type="entry name" value="Sulf_transp_TsuA/YedE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30574">
        <property type="entry name" value="INNER MEMBRANE PROTEIN YEDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30574:SF1">
        <property type="entry name" value="SULPHUR TRANSPORT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04143">
        <property type="entry name" value="Sulf_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="196"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="242"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="280"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="321"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="371"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="431"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="460"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="491"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="22"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="77"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="105"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8037004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="539" mass="57550" checksum="DC8D10C40C53BCFB" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H8L7</accession>
    <name>A0A9Q1H8L7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein bL9m</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">39S ribosomal protein L9, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_20635</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial ribosomal protein bL9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H8L7"/>
    <dbReference type="OrthoDB" id="5555409at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.5.10">
        <property type="entry name" value="Ribosomal protein L9, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000244">
        <property type="entry name" value="Ribosomal_bL9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009027">
        <property type="entry name" value="Ribosomal_bL9/RNase_H1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020070">
        <property type="entry name" value="Ribosomal_bL9_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036935">
        <property type="entry name" value="Ribosomal_bL9_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054302">
        <property type="entry name" value="Ribosomal_bL9m_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21368">
        <property type="entry name" value="50S RIBOSOMAL PROTEIN L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22078">
        <property type="entry name" value="Ribosomal_bL9m_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01281">
        <property type="entry name" value="Ribosomal_L9_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55658">
        <property type="entry name" value="L9 N-domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 11" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Ribosomal protein L9" evidence="9">
        <location>
            <begin position="88"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein bL9m C-terminal" evidence="10">
        <location>
            <begin position="153"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00035381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF22078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8036613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="249" mass="28541" checksum="98FEBCAC9EB6A58B" modified="2023-09-13" version="1">MSLSRVTSQMINLSGRGHLVNMINKLSGQLLETSQINQCQPTRNVVIVKRKYPIGVERQPGRELKPFKNRHKLYEFVEDTQHKTKKSVKVILTEDVPGIGLKYDIVAVRKGTVRNRLIPENLAVYASPENLEERAQAIKESAMGAKRLSLLAQETIQHMQSKPLRIAIHEDQEWKELERERVLHAFKMDGVHVPDYALKLPDEPVTEFGEYQIYVTVNGVKTVPVEMHVIKQEREKVEESDTDDINPES</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H9G3</accession>
    <name>A0A9Q1H9G3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">UDP-glucuronosyltransferase 3A1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_17955</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H9G3"/>
    <dbReference type="OrthoDB" id="5835829at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008194">
        <property type="term" value="F:UDP-glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050271">
        <property type="entry name" value="UDP-glycosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043">
        <property type="entry name" value="EG:EG0003.4 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043:SF145">
        <property type="entry name" value="FI06409P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8037201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="83" mass="9427" checksum="B0F7A08ECF7F2963" modified="2023-09-13" version="1">MYHRGINGVHEAIYHAVPMVIFPFLFDQFALAAAIRENGIGLVLRKHEITEERLELTIIIPRSYPCGPNARIIAMTCNERLHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H9S9</accession>
    <name>A0A9Q1H9S9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Arylsulfatase G</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_18901</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sulfatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H9S9"/>
    <dbReference type="OrthoDB" id="103349at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004065">
        <property type="term" value="F:arylsulfatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050738">
        <property type="entry name" value="Sulfatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024607">
        <property type="entry name" value="Sulfatase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000917">
        <property type="entry name" value="Sulfatase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42693:SF33">
        <property type="entry name" value="ARYLSULFATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42693">
        <property type="entry name" value="ARYLSULFATASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00884">
        <property type="entry name" value="Sulfatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00149">
        <property type="entry name" value="SULFATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sulfatase N-terminal" evidence="6">
        <location>
            <begin position="73"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8037950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="170" mass="18950" checksum="79C0D1C2304B112B" modified="2023-09-13" version="1">MQVTLITGSTQPNFVILLMDDVGYGDITSFWNPNNLPATTPFVDYMAANGISYTFFKTSVTFCLHIFFYFNFSIIRFTDFNSASSTCSPSRASLLTARLGARTGLTSVLLPSTRGGLPTNETTFGETFSKAGYKTGMIGKWHMGLRGDFHPYSRGKKWYMIESHVLETVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HBI8</accession>
    <name>A0A9Q1HBI8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_16875</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HBI8"/>
    <dbReference type="OrthoDB" id="115435at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053134">
        <property type="entry name" value="RNA-dir_DNA_polymerase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24559:SF435">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24559">
        <property type="entry name" value="TRANSPOSON TY3-I GAG-POL POLYPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="252"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8039226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="273" mass="30152" checksum="5E720E4D833047FE" modified="2023-09-13" version="1">MRRVEGDFHIELGRLEVPLAPKKSDVLPRRQVTTGATEEVIPSKVAKMVTCCVSDEPEGVQEGTICMGQEEGDTPGNNIPKETINKEDDVSQEPTIKVEDDESLVPTIKVKGSVRLEDGSPVKLPMGVNLEHLDDEKMRKVSVLLSKYSAAFSDGEYDLGFCDVIPHEINVTSDAPIRMPYRRIPPNEMSEVKELLQGMLDKGIIRRSASPYASPVVLVRKKTGVLRLCIDYRQLNAITLKDSFPLPRIEESRDDALGGAKQKGESPVSSLIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HBK7</accession>
    <name>A0A9Q1HBK7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_10275</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HBK7"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8043257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="105" mass="11741" checksum="64752386BB276AC5" modified="2023-09-13" version="1">MPHIKRKNPIVFGGGQRGSKVTWGQTLKTLLTQYLEMHYGMRKNPIIFGEGERGSKVTGGQTLKTLLTQYLEMRYGKRKNHIIFGGGERSSGVTGGQTLKIFLTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HCG6</accession>
    <name>A0A9Q1HCG6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SCAN box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_14430</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HCG6"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF13">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCAN box" evidence="2">
        <location>
            <begin position="18"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="112"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="376"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="127"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="376"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8040201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="422" mass="46394" checksum="BA8BF95AF61C503D" modified="2023-09-13" version="1">MDYDKVKKAILQAYELVPEAYRQKFRKYRKADTQTYVEFAYQKERHFDRWCASKKVSTFDTLRQLMLVEEFKRCVNDDIKTHLEENKADKLSEVANLADQYALTHKFGKVGQTQNKGQFNRPNKKYGNSSGTTGNSGSQSGSISPTNSGKKQPDSSRQKNDQVSSNKAAFKDISCGFCHKKGHTMANCFTLAKLKETETASNALAIAENSSEVVVTLRPNSKDKIKEEYLPFVSEGFISLDGNSAHPPVKIKILRDTGATQSLLLDGVLPLSDSTSTGANVLIQGVECGFISVPLHKINLKSDLVSGSVIVGVRPTLPVKGVSLLLGNDLAGGKVVANAILTDKPCDYNNTEQLEKEFPNLFPACAVTRAMSQKLAVDDNLKHPPSKSEDTVPKSGTSRESCKDDNLEHPLSKNEMILLGQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HD87</accession>
    <name>A0A9Q1HD87_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">G patch domain-containing protein 11</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Coiled-coil domain-containing protein 75</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_08107</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GPATCH11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HD87"/>
    <dbReference type="OrthoDB" id="786951at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025239">
        <property type="entry name" value="DUF4187"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000467">
        <property type="entry name" value="G_patch_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039249">
        <property type="entry name" value="GPATCH11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21032">
        <property type="entry name" value="G PATCH DOMAIN-CONTAINING PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21032:SF0">
        <property type="entry name" value="G PATCH DOMAIN-CONTAINING PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13821">
        <property type="entry name" value="DUF4187"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01585">
        <property type="entry name" value="G-patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01173">
        <property type="entry name" value="DUF4187"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00443">
        <property type="entry name" value="G_patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50174">
        <property type="entry name" value="G_PATCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G-patch" evidence="5">
        <location>
            <begin position="70"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="32"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="87"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="193"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="51"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="104"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="206"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="217"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00030688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8045162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="281" mass="32457" checksum="83432B514907C3B3" modified="2023-09-13" version="1">MAEEEEDDYMSDNFITQTESVRPGLAWGKHAKKYESEKRQKAAAAKNRLKPLKEREHEERQKGLNSALTSDNKGFAMLQKMGYKKGMGLGKQGTGRAEPVPIEVKADRGGLGREAEQKRKQQVRERLEVEAMRKRQKMMGQMKGDFRQRMRDKFDDKITGKDLYDSQKACQYLDESKGFGDPVVSWYWPPKPVEKKIKDDDEDDGGSSKDGLRTEAYESEEDEEEVEDEVDEDTFTPQEKLQQLTQYLRVTYHYCVWCGTSYEDAEDLQKNCPGSSHDDHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HDA8</accession>
    <name>A0A9Q1HDA8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Epimerase family protein SDR39U1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_12899</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HDA8"/>
    <dbReference type="OrthoDB" id="276721at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013549">
        <property type="entry name" value="DUF1731"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010099">
        <property type="entry name" value="SDR39U1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01777">
        <property type="entry name" value="yfcH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11092:SF0">
        <property type="entry name" value="EPIMERASE FAMILY PROTEIN SDR39U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11092">
        <property type="entry name" value="SUGAR NUCLEOTIDE EPIMERASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08338">
        <property type="entry name" value="DUF1731"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="1">
        <location>
            <begin position="3"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1731" evidence="2">
        <location>
            <begin position="245"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8041954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="294" mass="32622" checksum="E13A2A8C7258E460" modified="2023-09-13" version="1">MNIVIGGGSGFIGKHLGKQLIQKGYSVINVGRTAGKGKLSWDEIKSDGLPPCKAVVNLAGQNLFDPVKRWSDQFVKDCFDSRLKTTTALAEAIVKMPEPPEAFICTSATGYYPFSWTEEFTEYYDGPPADFVEQLVPKWEARVTLPETHENVRRVIIRTGLVMGKDGGMLQQLRLPYYFGLGGVVASGKQYMPWIHVDDIAGIFVHAIESNVTGILNGTAPNPVTNYEFTKAFGAALWRPTIFWVPEFAVNIMLGKERAPGMILGNKVIPQRVLETGYKFKFEDIHEALKDLCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HFJ3</accession>
    <name>A0A9Q1HFJ3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PRKR-interacting protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_11385</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8044040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HFJ3"/>
    <dbReference type="OrthoDB" id="10067079at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004860">
        <property type="term" value="F:protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009548">
        <property type="entry name" value="Prkrip1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13507">
        <property type="entry name" value="PRKR-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13507:SF0">
        <property type="entry name" value="PRKR-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06658">
        <property type="entry name" value="DUF1168"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="144"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="160"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8044040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="185" mass="21325" checksum="730C4035631BA913" modified="2023-09-13" version="1">MDEKKDGPKKHVHKKNDKPLVLPKSLAEKQRIHLEKLMSNPEKPVSVPEKRKEWNPRAPPEFVRDVMGSSAGAGSGEFHVYRGYRRREMARQTYLDAKHEKEQKDAEFEEKLEKNKMEAESKTAKKRAKRQRKKQKLLAKKQKLQKEKLGAIDENAKSDSEDEADSDGEEKEDDAEVNCFVIGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HFQ0</accession>
    <name>A0A9Q1HFQ0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_11614</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8044220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HFQ0"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8044220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="168" mass="17451" checksum="DF91411FBA689542" modified="2023-09-13" version="1">MEDVTRGLIGRRVCGGIEVGFGILLVIGAVQSANFISILSGAFIVLTGILGTVSKTRNLVIGYLVTGIICAIFLAISLILTGIVITSLDDEYNLDGTSQAILIAGVVIVALGLIVTVLGVIFACLPLCGTTQEQGTVQYNHNTSPNIVGPPAYNYGNTVYPNTKQGPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HGK7</accession>
    <name>A0A9Q1HGK7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">REST corepressor 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_08021</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the CoREST family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HGK7"/>
    <dbReference type="OrthoDB" id="10064338at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000118">
        <property type="term" value="C:histone deacetylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1880:FF:000001">
        <property type="entry name" value="REST corepressor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000033">
        <property type="entry name" value="REST corepressor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1240.50:FF:000002">
        <property type="entry name" value="REST corepressor isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1240.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000949">
        <property type="entry name" value="ELM2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049048">
        <property type="entry name" value="REST_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051066">
        <property type="entry name" value="Trans_reg/Corepressor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16089:SF28">
        <property type="entry name" value="REST COREPRESSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16089">
        <property type="entry name" value="REST COREPRESSOR COREST PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01448">
        <property type="entry name" value="ELM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20878">
        <property type="entry name" value="REST_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01189">
        <property type="entry name" value="ELM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51156">
        <property type="entry name" value="ELM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="ELM2" evidence="10">
        <location>
            <begin position="44"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="11">
        <location>
            <begin position="131"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="12">
        <location>
            <begin position="359"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="11">
        <location>
            <begin position="359"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="188"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="276"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="429"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="27"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="282"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="436"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="455"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="465"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8045090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="548" mass="60581" checksum="242D81F05D7F7A97" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HHL4</accession>
    <name>A0A9Q1HHL4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Septin-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_05721</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HHL4"/>
    <dbReference type="OrthoDB" id="2386367at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030379">
        <property type="entry name" value="G_SEPTIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32046:SF14">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32046">
        <property type="entry name" value="G DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00735">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Septin-type G" evidence="3">
        <location>
            <begin position="53"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="418"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU004560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8046889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="574" mass="66096" checksum="250A66C8F91B9919" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HIQ7</accession>
    <name>A0A9Q1HIQ7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_05090</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HIQ7"/>
    <dbReference type="OrthoDB" id="2505440at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8046426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="104" mass="11453" checksum="9687461B6694BEF8" modified="2023-09-13" version="1">MRSDTLPTVVKSPSTNLTLFDNAASSPPFTLKEAERKLVAASARSPGEEKPSEKGAVLGKLYQKPIEESDTRFSLGGKHLVEIRRYREVPCINIREYFGGAAED</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HIZ9</accession>
    <name>A0A9Q1HIZ9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein zwilch</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_06545</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores. Its function related to the spindle assembly machinery is proposed to depend on its association in the mitotic RZZ complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the RZZ complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Chromosome</location>
            <location evidence="1 9">Centromere</location>
            <location evidence="1 9">Kinetochore</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the ZWILCH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HIZ9"/>
    <dbReference type="OrthoDB" id="5556307at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990423">
        <property type="term" value="C:RZZ complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007094">
        <property type="term" value="P:mitotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034501">
        <property type="term" value="P:protein localization to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018630">
        <property type="entry name" value="Zwilch"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15995">
        <property type="entry name" value="PROTEIN ZWILCH HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15995:SF1">
        <property type="entry name" value="PROTEIN ZWILCH HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09817">
        <property type="entry name" value="Zwilch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4 9" id="KW-0132">Cell division</keyword>
    <keyword evidence="8 9" id="KW-0137">Centromere</keyword>
    <keyword evidence="3 9" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6 9" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="5 9" id="KW-0498">Mitosis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU369076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8047520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="376" mass="43810" checksum="B0CA84BDE1C99D14" modified="2023-09-13" version="1">MDDDGIPKSSITMEWEWPRVRELLETPTGQQCTLKVKKGKGEILHTTPERNLRELELLKSFITALSTDEICWPTRDENTQSVEEIMKIYLQDLKENPVKYVNEKYVETFEEVGHYEEDVLGRRFLDFTERIWLIIAHNATSYNDMNVVLKLVEEEFRTGHLQPMIYPDNTSSLACKIREFYLNAETPGQWKEFSLPDGSSLELLIGIALDKIRRDFTTYLIDNSLCLGHQISWYVPTKEVDLRDQAHRLQCLHHVLELALLTNGYLSRFISSNLDVIKTAILHYQSHHPDEEFTVIAPGIPKELKTLCQRSIPIQWQVELKETGPRENTLIVNLTRNPPFSHISSPQFDTTAEDLQDDEFISYYITKVRQHSVRFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0Y898</accession>
    <name>A0A9Q0Y898_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF6570 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_45264</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010002253">
        <property type="protein sequence ID" value="KAJ8017378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0Y898"/>
    <dbReference type="OrthoDB" id="6141723at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046700">
        <property type="entry name" value="DUF6570"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20209">
        <property type="entry name" value="DUF6570"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6570" evidence="1">
        <location>
            <begin position="245"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF20209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8017378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="486" mass="55404" checksum="26CEFD7A800A5231" modified="2023-09-13" version="1">MLLHNYCLRKCLSCEDAQKIYFPPNIIYFSVYYSIKVLKTQQVTFLSSDLQHLFATSKWWILTYKKHCVSLLYNGAIHVFDPYCHKDPNLKSGSYVVSLKNIQEFNMFMCGLLSTIFQDFSASDICYLHCVNILQPKILDGINIEWCNVTPDVTMPLVYDSYKSIENQSNDALKDLIDVFHKAVQQGPVYICSCCEQLWFKRSVENVKTVSSKIVAHLQWCITNILSFDNEKYLCTTCIRNLKQGDKPLLATSNNLSFGNIPPALVDLYPLEERLVAARIPFMQIRALPCGDQLKLKGSVVNVPANVSTTISVLPRTLNEAEIITLRLKRKLEFSHDYLFDNVRPFKVLEAAIWLVNNSPLYKAEKISLLDDYNPSNMFSNENCTGLSVSSPQHLDEIHCPNPQLPETNLDNQPDEEDNWTEIDSSNPSITGNLETLLDDQFPTTVLPKLVLSIAPGEGNHPLGIFKTNIQRSFHFPHYLVEMLDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0Y932</accession>
    <name>A0A9Q0Y932_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_44091</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000631">
        <property type="protein sequence ID" value="KAJ8018103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0Y932"/>
    <dbReference type="OrthoDB" id="6819336at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8018103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="142" mass="16496" checksum="086C577AE8FAD38C" modified="2023-09-13" version="1">MFMQWPNCFEKIITYAEKQVDWKQLLHFSGNLENDWYQHSCIFGSCEENTAPFLLALGKQEDPEQVFVIIERQTVACRSLVKGLDLSFKLFCILDIDYPWECQNLWDFIQKFIYGLGKGKGREKSVPSVSLLINFLTPSEAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YA64</accession>
    <name>A0A9Q0YA64_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_43960</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000556">
        <property type="protein sequence ID" value="KAJ8018190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YA64"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8018190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="220" mass="24653" checksum="86EFB16B24FC0B41" modified="2023-09-13" version="1">MKFSPVEEFSLLTVHFFLQEGVPNPHPQLLWSWPWRLWLVRPWKGSGRGQQKLTQWVMLLLKCHRNHHKTSETSTQLPPNASAVGSTAYSEPATTTLPSAEPSSTETGSLVALQQQVNLLQSQVEMLGSQPTSLQRATELRQLKFQEELLKLEKKEGGPPESVSGASEENACCPLNLSHHIPCSGQCLSSRTHYSNLSIPPLLSILKKFYLGFEVMHIFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YD25</accession>
    <name>A0A9Q0YD25_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_42201</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000040">
        <property type="protein sequence ID" value="KAJ8019286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YD25"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8019286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="186" mass="20558" checksum="27A61DC8E00727CE" modified="2023-09-13" version="1">MPHIERKNPIVFGGGQRSPGVTRGQTLKTLLTQYLEMHYSKRKNPIIFGGGHRSSGVTGGQTLKTSLTRYLKMHYGKRKNPIIFGGGHRSSGVTGGQTLKTSLTRYLKMPDIERKNPIVFGGGSKVTRGQTQKTLLTQYLKMHYSKRKNPVVFGGGQRSPGVTGGRTLKILLTQYLEVGSLDEFHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YEC7</accession>
    <name>A0A9Q0YEC7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_41720</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8019931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YEC7"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040497315" evidence="1">
        <location>
            <begin position="24"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8019931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="83" mass="9393" checksum="06466A4D331F7007" modified="2023-09-13" version="1">MNTKTVLLALALVLALVISTSEARNYYYAPGNRPNSGRQRNRGNKAFKNPFLKRFMEEYYGTDKAPYVNPNDVGVYGDDLDVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YFV3</accession>
    <name>A0A9Q0YFV3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_38942</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YFV3"/>
    <dbReference type="OrthoDB" id="5986179at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1310.20">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8021670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="154" mass="17662" checksum="D69A0BC393C318BC" modified="2023-09-13" version="1">MSAYSQDFTETPGTSAELGRRQVRQVYLITYSQADHDKVPTRLSFAEKGVESFRHAHIEVRQWACSLEKHQKQGHHFHMAVKLEGLHRWLAVKNDLSAQYGLTCHFSSLHSSYYTAWKYVTNEDTENIQSEGHPNLVNPADGRQAQEGAFFKPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YHY9</accession>
    <name>A0A9Q0YHY9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_37921</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YHY9"/>
    <dbReference type="OrthoDB" id="10654122at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="75"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="135"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="136"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="195"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="209"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="256"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8022897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="270" mass="29640" checksum="401241D18E2CB5A7" modified="2023-09-13" version="1">MGSRPSSQRSSNDQSNGSVGSEAERELRTQNAKLQEKIAELERLQVPENVYQENEKLRQENEFLKQQLSENCRSISADGISCPQTDSKDNLSQRDNKSSIDLSFYSQTEDIAKDAKSEKSESDLNGNFLEKEKLNVSPSLSPSVSPSVSPAVSPVVPKKEKKPRRINPNSIPQRISDIDAYGQGLPLPDAELLRKNIRSSRLRKQRNDSGNSTGSSAGLSTGSVRSERAPSSSPERTDQRDVFVGVETLSPGVDESDSRSGSEAKEDLLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YJH3</accession>
    <name>A0A9Q0YJH3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_36181</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YJH3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8023678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="53" mass="5891" checksum="D73CAF4EF5C4D1FE" modified="2023-09-13" version="1">MNFNLGLLMPYSRRKNPIVFSGGQRSSGVTSGQTLKTLLTRHLKVGSLDKFHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YL63</accession>
    <name>A0A9Q0YL63_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_34524</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YL63"/>
    <dbReference type="OrthoDB" id="432483at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="91"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="146"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="167"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="208"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8024580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="341" mass="37691" checksum="1B3663108598B322" modified="2023-09-13" version="1">MDVGNPLDDVFKGTSFLCSVLVAPVKSESQVIIMYILNFEDISDTKPNNVAAGETDAVGDGTVLYPQQQQQPGYKWKRGRNNHRTFRLRLPSIYRSQQRMGVQSNSQQDLSQKDPENPPSEPESSMPLLRIDSGLKDGPSLKGGPSLNSLNPPGEPEQTTELLTVDGAEDSIDDSDPFQVESSGSLRNDDETPVTEKGLPNSHDHLTRSMSNTTWPNHSFHTTGPNIRKAASLENLRFSARRRHMSVPNDTFAKNHIVNDNIQTAVSESDLTKYKQHRKELYGSSVCNGDKSSLNSTANSQSGSALATKKMFSEVTTNIGGKVTQRHEVFQKRWGRGIYEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YN71</accession>
    <name>A0A9Q0YN71_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Prominin-1-A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_33085</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the prominin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YN71"/>
    <dbReference type="OrthoDB" id="6229420at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005902">
        <property type="term" value="C:microvillus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071914">
        <property type="term" value="C:prominosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015485">
        <property type="term" value="F:cholesterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008795">
        <property type="entry name" value="Prominin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22730">
        <property type="entry name" value="PROMININ PROM PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22730:SF1">
        <property type="entry name" value="PROMININ-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05478">
        <property type="entry name" value="Prominin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040241165" evidence="9">
        <location>
            <begin position="25"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="108"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="417"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="466"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="777"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="275"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="555"/>
            <end position="582"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8025507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="898" mass="99671" checksum="A6BD4C1AF2B0ECD0" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YPK3</accession>
    <name>A0A9Q0YPK3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Integrase core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_35319</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8025185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YPK3"/>
    <dbReference type="OrthoDB" id="10045093at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058913">
        <property type="entry name" value="Integrase_dom_put"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46791:SF13">
        <property type="entry name" value="CLR5 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46791">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24764">
        <property type="entry name" value="rva_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase core" evidence="1">
        <location>
            <begin position="121"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF24764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8025185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="336" mass="39307" checksum="48E9DEF7B5790368" modified="2023-09-13" version="1">MANVRGRVIISYFDLGLSYDEILSFLRDLHGIQISKRHLKRILGRLGLFRRKNHDDVVDVALRIEEEIESSGRLHGYRWMHNKLKNNGFNTSKETVLELLRILDPDGVQIRRRRRLRRRRYNGHGPNFIWHMDSYDKLRPYGVCINGCIDGYSRRVLWLEAYNTSSDPNLIAGYFMKKVQELGGTPRVVRADMGTENGTVRDMQIFLRWDDADQLAAERSFMYGKSTSNQRIEFLWGVLRKQTAQFWMDLFGSLRDEGLFDGGYIDKALIQFCFLRLIQKELDSFVTQWNSHRIASSRMSNGPFGRPIVMYSLPHLNNTHDFLHDVPIEEVDVCID</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YR39</accession>
    <name>A0A9Q0YR39_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Neuropeptide SIFamide receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_33678</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YR39"/>
    <dbReference type="OrthoDB" id="2132067at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24243">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24243:SF233">
        <property type="entry name" value="THYROTROPIN-RELEASING HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="45"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="76"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="9">
        <location>
            <begin position="24"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8025965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="99" mass="11223" checksum="2966E0AB385DF685" modified="2023-09-13" version="1">MVSFTFLSITTVIYAVLFLLGTIGNVLVITVIYKHKEMRSSTNYFLVNLSLADLLVLLICMPVSLSEMYLGEYFPFGRIMCEFISFIFSSAFLSPSVFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YR63</accession>
    <name>A0A9Q0YR63_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_31994</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YR63"/>
    <dbReference type="OrthoDB" id="6343797at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46585:SF1">
        <property type="entry name" value="CHROMO DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46585">
        <property type="entry name" value="INTEGRASE CORE DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8026994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="150" mass="17404" checksum="C623B34AC7CC4F17" modified="2023-09-13" version="1">MWKYFTHRNTLKYIDILPNLVKGYNHSYHRSIKLRPVEVTKDNEDWVWRTLYPGININASFKFNVGDRVTISKSKLKRDICQTGAKKYLSLLRGKLNPSLCTNLLTGVENPSREIFTRANCKKLSPSGCPLQSREGIEEKKTQWKGRIFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YTI0</accession>
    <name>A0A9Q0YTI0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">G-protein coupled receptor GRL101</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HOLleu_31512</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Nanhai2018</strain>
            <tissue evidence="16">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="5979691at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008528">
        <property type="term" value="F:G protein-coupled peptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009755">
        <property type="term" value="P:hormone-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00057">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000421">
        <property type="entry name" value="FA58C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24372:SF77">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24372">
        <property type="entry name" value="GLYCOPROTEIN HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00754">
        <property type="entry name" value="F5_F8_type_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00261">
        <property type="entry name" value="LDLRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00231">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01285">
        <property type="entry name" value="FA58C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01286">
        <property type="entry name" value="FA58C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50022">
        <property type="entry name" value="FA58C_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1629"/>
            <end position="1648"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1669"/>
            <end position="1693"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1713"/>
            <end position="1734"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1754"/>
            <end position="1776"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1804"/>
            <end position="1829"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1853"/>
            <end position="1877"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1889"/>
            <end position="1911"/>
        </location>
    </feature>
    <feature type="domain" description="F5/8 type C" evidence="13">
        <location>
            <begin position="6"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="F5/8 type C" evidence="13">
        <location>
            <begin position="156"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="F5/8 type C" evidence="13">
        <location>
            <begin position="323"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="12">
        <location>
            <begin position="837"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="14">
        <location>
            <begin position="970"/>
            <end position="1093"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="1641"/>
            <end position="1908"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1923"/>
            <end position="1948"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1923"/>
            <end position="1940"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="837"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="891"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="1242"/>
            <end position="1254"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="1249"/>
            <end position="1267"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="1261"/>
            <end position="1276"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="1289"/>
            <end position="1301"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="1296"/>
            <end position="1314"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="1308"/>
            <end position="1323"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="1454"/>
            <end position="1466"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="1461"/>
            <end position="1479"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="1473"/>
            <end position="1488"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="1493"/>
            <end position="1505"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="1512"/>
            <end position="1527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ8026622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2019" mass="226154" checksum="960BE1A55B1EA133" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1B9N6</accession>
    <name>A0A9Q1B9N6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Membrane frizzled-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_43578</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000367">
        <property type="protein sequence ID" value="KAJ8018440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1B9N6"/>
    <dbReference type="OrthoDB" id="19606at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003410">
        <property type="entry name" value="HYR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24251:SF40">
        <property type="entry name" value="CUB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24251">
        <property type="entry name" value="OVOCHYMASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00261">
        <property type="entry name" value="LDLRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50825">
        <property type="entry name" value="HYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="582"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="6">
        <location>
            <begin position="31"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="6">
        <location>
            <begin position="194"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="HYR" evidence="7">
        <location>
            <begin position="495"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="643"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="718"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="168"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="317"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="324"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8018440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="739" mass="82672" checksum="8F83686349046866" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BAQ3</accession>
    <name>A0A9Q1BAQ3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_43989</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000571">
        <property type="protein sequence ID" value="KAJ8018169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BAQ3"/>
    <dbReference type="OrthoDB" id="10052386at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8018169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="193" mass="22575" checksum="AFD1B556F86E2773" modified="2023-09-13" version="1">MPALPAVAHSSPMDESEEQALRYCSGYVLQKLMKKYKRMTSSKTAQQFCEVLTSWNVPVEDEGESVIEFTSLWLEMQNRGGLFHIDNNVYTFFKSLEVVVRSHVHKENIDVFKQSDIQVALFTRIQESHSVWINWNNICSSLPEQGKLKLFEEVVKCFLKMRCEAFLRVYVMIRKAIENSSKERRKKSKKRAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BBK9</accession>
    <name>A0A9Q1BBK9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cation-transporting ATPase</fullName>
            <ecNumber evidence="13">7.2.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HOLleu_39177</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Nanhai2018</strain>
            <tissue evidence="17">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Membrane</location>
            <topology evidence="1 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type V subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.-" evidence="13"/>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BBK9"/>
    <dbReference type="OrthoDB" id="48943at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019829">
        <property type="term" value="F:ATPase-coupled monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140358">
        <property type="term" value="F:P-type transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015203">
        <property type="term" value="F:polyamine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000023">
        <property type="entry name" value="Cation-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006544">
        <property type="entry name" value="P-type_TPase_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047819">
        <property type="entry name" value="P5A-ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01657">
        <property type="entry name" value="P-ATPase-V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45630:SF8">
        <property type="entry name" value="CATION-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45630">
        <property type="entry name" value="CATION-TRANSPORTING ATPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12409">
        <property type="entry name" value="P5-ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 13" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="186"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="214"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="383"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="423"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="888"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="914"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="952"/>
            <end position="980"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1000"/>
            <end position="1019"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1031"/>
            <end position="1052"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1072"/>
            <end position="1092"/>
        </location>
    </feature>
    <feature type="domain" description="P5B-type ATPase N-terminal" evidence="16">
        <location>
            <begin position="20"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="15">
        <location>
            <begin position="151"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="14">
        <location>
            <begin position="250"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU362082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF12409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ8021861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1132" mass="126372" checksum="61AE2F6E50C908A9" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BBX7</accession>
    <name>A0A9Q1BBX7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_39438</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8022056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BBX7"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031766">
        <property type="entry name" value="RRM_occluded"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16842">
        <property type="entry name" value="RRM_occluded"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="350"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="131"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="62"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="89"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8022056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="444" mass="49941" checksum="8DA411073E1FF4EB" modified="2023-09-13" version="1">MTKEKGKSKRLVMTEPTFKKRKGLSAEMSGTAVKRSPLSKTAHKRKSLSGREILRQRKLLKQRQEDDLSRRLFPYVEEKEASSQDSPKHSSKKVSKVHKGLAGKKSKGSLVSSPELDVIKNAEETFDDSMNSTLPFEERNNENVENDNSETEDNIVSFLRTPGLNGKTDREEDMDKTPLLTKKTPMPEGDEQWLYSSKKKRNKARRLKGKYLLKCDNLPKGTTVHYLGLFLAECDLFPKIVVKQAVRAGRGRKRVSNHAAKGRWVRTEGISSHYVTSFDANGAANGASGAAAGAGGGVADIYAILYCNTEEDRDKLLQLDEITMPSVTNSKDRVLRIVPMLTKQDTRSIRALRISNLAEGVTEQDLRDFFERKEMSIYKVYLYPNDRCAIVLFRRANDAKKALLSEGSKLLKGSEMEFHPNVGQMMYGERGELLPMDDPSKKSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BBY9</accession>
    <name>A0A9Q1BBY9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_39478</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8022086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BBY9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="105"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="127"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8022086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="145" mass="16259" checksum="341BEDED1FBA964A" modified="2023-09-13" version="1">MIQLPQSNLPHVVYNASYQGEVLLTSMTIRDDTLAAELSDELKEQVSTSYQLLSVFVPLVIGMTEEHAVLNFHHFTNSTKKITYFFGTSTSDFTQVDSIEEAENFTGSSPDKYKGSCRKIRSKSRCPRTSNPKTKNNKTVSVART</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BCJ9</accession>
    <name>A0A9Q1BCJ9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_37730</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BCJ9"/>
    <dbReference type="OrthoDB" id="5981530at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008528">
        <property type="term" value="F:G protein-coupled peptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009755">
        <property type="term" value="P:hormone-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24372:SF77">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24372">
        <property type="entry name" value="GLYCOPROTEIN HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="38"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="76"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="118"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="159"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="235"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="279"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="314"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="9">
        <location>
            <begin position="50"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8022746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="352" mass="38777" checksum="91B019C97BF35D09" modified="2023-09-13" version="1">MSDSFSICCFLNKTTECATPPSPFATCDGILYSWVLRLSAWIISVAAIVTNLMVLIGRWKAKLNATTAKPTENIFLANLAVADFLMGAYLLAIAIADASFGSDYFLYSKTWRTGTTCGIIGVIGVLSSVVSLLDLTVMTIERFICIVFPFGKIRLNPKWGKIICSIIWIFGFTVAILPTVSSDSLPNFYGYTDVCLGLPIVAIPQKVSKVVEKQGLLYQQTGYTSEAMATWKYSAILFTYLSSICLLIVTICYVSIFVSVLKTRRSSHRASGRKDEVKLATRISIIVGTDMLCWLPLITLGVLSQCGIQIPSKVNPWLVVCVVPINSALNPFLYSHRILKKRKKNVPSYIQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BCK3</accession>
    <name>A0A9Q1BCK3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Bone morphogenetic protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_41278</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8019622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BCK3"/>
    <dbReference type="OrthoDB" id="5975444at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000013">
        <property type="entry name" value="Membrane frizzled-related protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24251:SF37">
        <property type="entry name" value="CUB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24251">
        <property type="entry name" value="OVOCHYMASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CUB" evidence="4">
        <location>
            <begin position="43"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="4">
        <location>
            <begin position="103"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8019622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="217" mass="23724" checksum="A91E4AF3A28B0B70" modified="2023-09-13" version="1">MSMEMTSTVPISSPFLTDISSMSLFWILNWKLQRLVAMINYRIFDGIDNTTNEIAELCGEILPDPVFTSGSDSFIEFTSDASVTRTGFKAVITSALNVPQTDCDNIYTDESETVTSPNYPDDYGINEDCGNTIQAPAGRVIRLTFQTFHLESTSATCTYDSVNIYEGDSTSSPLLAGPFCGSTIPPPVTSTGNVMYVYFESDGSVQESGFLATFEFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BDK1</accession>
    <name>A0A9Q1BDK1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_37729</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BDK1"/>
    <dbReference type="OrthoDB" id="10037933at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8022745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="79" mass="9198" checksum="480D690F771E24DD" modified="2023-09-13" version="1">MPVTCSDCGRSFSVLPRLSTDLRASMTEPRLSALAMMHVHYDMDIDLNVVVNLFARYHPEKLRKVHLFFSGQYKSYFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BDR5</accession>
    <name>A0A9Q1BDR5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_38932</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BDR5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8021663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="122" mass="13166" checksum="309336EB58539620" modified="2023-09-13" version="1">MIKGAASQEYLSGLEQAGLRRSSCSYAEASKRVFSNKIRERGSPYSDSIDENTTARHGHPQLVLNESGAAQSSCPTRAETSDQNTCRGNSSQFQVHLLNIVGTAYKLQTVLDSSKTVGAQVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BE52</accession>
    <name>A0A9Q1BE52_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">EF-hand calcium-binding domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_34369</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BE52"/>
    <dbReference type="OrthoDB" id="191686at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028846">
        <property type="entry name" value="Recoverin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23055:SF60">
        <property type="entry name" value="CALAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23055">
        <property type="entry name" value="CALCIUM BINDING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00450">
        <property type="entry name" value="RECOVERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="64"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="100"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="145"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8024456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="212" mass="25051" checksum="614E68B32B41596D" modified="2023-09-13" version="1">MKDKKHPQMPVCQENYRNTHFTKEEYEALENMFKDLTKDNIQTKAGKLDRSQFRDVLHATFKMTDDMILDRVFRAFDEDSDSYINLSEWIHGLSIFLRGDLTERAKFCFDVYDLNGDSFISREEMFHLLKYSLMKQPTEEDPEEGTKELVEILLKKMDYDHDGRLNLTDYETAVKDDDLLIEAFGACLPEPQTAKTFLEYAFTVPKVRSRYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-10-08" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BFI0</accession>
    <name>A0A9Q1BFI0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Endonuclease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_34653</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10064731at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
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        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09274">
        <property type="entry name" value="RNase_HI_RT_Ty3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000115">
        <property type="entry name" value="Polyprotein of retroviral origin, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000032">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 297-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon gypsy-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DNA/RNA_pol_sf"/>
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        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054465">
        <property type="entry name" value="Integrase_p58-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041577">
        <property type="entry name" value="RT_RNaseH_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Integrase_p58_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17919">
        <property type="entry name" value="RT_RNaseH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="4">
        <location>
            <begin position="855"/>
            <end position="1013"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase" evidence="3">
        <location>
            <begin position="1269"/>
            <end position="1481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="94"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="350"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="621"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="688"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="350"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="389"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="632"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="657"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="704"/>
            <end position="725"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8024680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1622" mass="186028" checksum="51F4B657AD1BD103" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BFT9</accession>
    <name>A0A9Q1BFT9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Sodium-coupled monocarboxylate transporter 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_36084</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BFT9"/>
    <dbReference type="OrthoDB" id="6132759at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11492">
        <property type="entry name" value="SLC5sbd_NIS-SMVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="entry name" value="Sodium/glucose cotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051163">
        <property type="entry name" value="Sodium:Solute_Symporter_SSF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00813">
        <property type="entry name" value="sss"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42985:SF40">
        <property type="entry name" value="LD47995P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42985">
        <property type="entry name" value="SODIUM-COUPLED MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="10" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="55"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="85"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="129"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="160"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="279"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="340"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="383"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="409"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="441"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8023599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="498" mass="53544" checksum="34EE54E5C4455755" modified="2023-09-13" version="1">MSVPGEVATFVVWDYVVIAGVLLVSASIGIFYALAGGRQRTSREFLLADRNMKPVPVALSLSASFISAVTVLGTPAEIYRYGTMYWLICFSFIITGIIVARTFMTIFYKHEITSTNEYLERRFHKNVRYLGTALFFLQMILYLGIVIYGPALALNAVTGLSLWGSVLTTGIICTFYTSIGGLKTVVWTDAFQVCIMLSGLLAVIIEGSVQLGGLREAWQIADSRGRIDFWNFNPNPTIRHTFWSVAIGGAFNWTATYGTSQAQVQRYLSCGKKSTANRALGLAVCFMIILLSVSCLTGVVMFAYYADCDPFTAGYVSASDQLLPYFVMDLFGDKPGLPGLVTSCVFSAALSTVSSGLNSLAAMAGEDIVKSISPNISDSKYTIITKLIAFSFGAVAILVTYLASQFDGILQATVSIFGLVGGPLLGLFSLGVFFPCVNSYGAFFGTLVGVSMSFWVGVGALLYPPGTNPPSLSTEMCYMNVNDTLSPSTTVSAGSDET</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BGB4</accession>
    <name>A0A9Q1BGB4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ras association domain-containing protein 8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_36060</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BGB4"/>
    <dbReference type="OrthoDB" id="10051571at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033593">
        <property type="entry name" value="N-RASSF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000159">
        <property type="entry name" value="RA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15286:SF6">
        <property type="entry name" value="GH01133P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15286">
        <property type="entry name" value="RAS-ASSOCIATING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00314">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50200">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ras-associating" evidence="2">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="624"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="83"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="133"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="184"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="624"/>
            <end position="641"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8023582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="675" mass="79760" checksum="62D5394E134A3B8D" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BGV5</accession>
    <name>A0A9Q1BGV5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cell agglutination protein pfl3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_35421</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8025263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BGV5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="133"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="181"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="227"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="278"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="324"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="365"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="406"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="452"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8025263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="484" mass="46510" checksum="70EC768962ED1BC5" modified="2023-09-13" version="1">MLVTAGVVTTLVVVLVVVSAGVVTMDVVVKAGVVTAEVVASGVVTAILVTAGVVTSLVVVLVVVTAGVVTLDVVVKAGVVTAEVVVSGVVTATLVTAGVVTSLVVLIVVVTAGVVTLDVVVKAGVVTAEVVASGVVTAMLVTAGVVISLVVLIVVVTAGVVTLDVVVKAGVVTAEVVASELVTSILVTAGVVTTLVVVLVVVSAGVVTMDVVVKAGVVTAEVVASGVVTAILVTAGVVTSVVVVLVVVTAGLVTLDVVVKAGVGTAEVVATGVVTAKLVTAGVVTSLVVVLMVVTAGVVTLDVVVKAGVVTAEVVASRVVTSMLVTAGVVTSLVVLIVVVTAGVVTLDEVDKAGVVTPEVVASGVVTAILVTAGVVSSVVVLLVVVTAGVVSFDVVVKAGVVTAEVVATGVVTAILVTAGVVISLVVLIVEVTAGVVTLDVVVKAGVVTAEVFASGVVTVVVVTAGVVTSLVEICVVVIAGVVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BGW1</accession>
    <name>A0A9Q1BGW1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">DNA damage-regulated autophagy modulator protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_36801</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DRAM/TMEM150 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8024152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BGW1"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019402">
        <property type="entry name" value="CWH43_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050911">
        <property type="entry name" value="DRAM/TMEM150_Autophagy_Mod"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21324:SF2">
        <property type="entry name" value="EG:22E5.9 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21324">
        <property type="entry name" value="FASTING-INDUCIBLE INTEGRAL MEMBRANE PROTEIN TM6P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10277">
        <property type="entry name" value="Frag1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="52"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="89"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="119"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="155"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="193"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="CWH43-like N-terminal" evidence="7">
        <location>
            <begin position="8"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF10277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8024152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="245" mass="28479" checksum="E13245C2236EFA6B" modified="2023-09-13" version="1">MFEIQMNWSPVLALIFLLAALFVPYTIGVCSGHVRAWFPFIHELGANDPDRCVFTALLNLVALLLFVNVNDQRNVINEILVWQTQSSYLYGFSFWCGALACFGLFLMANFPAINNKLPFFIHCCGAVLFFCGSPVYCGILTLFSYNETKYYDENWFPFIIRCQFLFLYLIALIGYIVFWYFGKSQPQKVIIKISALCQWLLPITVIAFFLTYEMEFWKLHGGQIHHKITVNRYQKTNANLKLSWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BHU8</accession>
    <name>A0A9Q1BHU8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Coiled-coil domain-containing protein 28B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_31560</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BHU8"/>
    <dbReference type="OrthoDB" id="9977011at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025271">
        <property type="entry name" value="CCDC28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13400">
        <property type="entry name" value="CHEMOKINE C-C MOTIF RECEPTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13400:SF4">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 28A-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13270">
        <property type="entry name" value="CCDC28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="93"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="176"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="9"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="109"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8026658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="244" mass="27433" checksum="38E4D7FF884526D5" modified="2023-09-13" version="1">MATATRLAPHHESPRTGRHVGDSSRLRRPASLTSGVPPLQGTFVRKHTRGHSLGFVSSPSTFIQQGPYPPYHALPRDYRLSMAGPPFSMGASVHRGYGKAARQSTVTGRHVEKNKQKEKQKNKSDKEKGNVEHSFITSSSDIAEMQGDLLRLQEDFNSGKLQAFDQQCSFEKMDNIRDLQEKLARLHFRMDEKLQAEGLHPTETIEVANENMDHLLSNLDQLRIAIGSLHPPREDVAYPPDHRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BIP8</accession>
    <name>A0A9Q1BIP8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_32435</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BIP8"/>
    <dbReference type="OrthoDB" id="10068277at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="195"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="195"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8027321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="258" mass="29530" checksum="1A6F75BD3C5DB517" modified="2023-09-13" version="1">MDPRLQHPFTCVKAGPTQCGKTVFLNQLLSHAEELIRDPTQVILWFYGEYQSLYDQLAETLANEPLKVKVLPETSSSVQPGDTLQKRVIERDVVESIPRAFKSKTNQLLGKIASNDSGLDWTNRGELVVNDHTVKGSRIVDLVNDMMRKRRRATPIGMDEFTKALARINTTRELIGNLDRWQDLSRWRDLGDAPRKVTERPLHEEVSEVSEDNDGNDQTFYAKAYHLPTKKRPGISPRKSKKAMRAREKYLILFNTVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BJ45</accession>
    <name>A0A9Q1BJ45_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Chloride intracellular channel protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_32710</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the chloride channel CLIC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BJ45"/>
    <dbReference type="OrthoDB" id="1935530at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053823">
        <property type="entry name" value="CLIC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43920:SF5">
        <property type="entry name" value="CHLORIDE INTRACELLULAR CHANNEL CLIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43920">
        <property type="entry name" value="CHLORIDE INTRACELLULAR CHANNEL, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22441">
        <property type="entry name" value="CLIC-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="GST N-terminal" evidence="6">
        <location>
            <begin position="18"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8027542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="226" mass="25687" checksum="229BAF60764BD793" modified="2023-09-13" version="1">MDNHSSEDGSKMITLFIKGGVAHTVGDCPLCQRVYMVLKLKDLNFRVMAVNTNAPPTEYKEIAGTKKPPILVDGEIIHNDVAQIVEYLDASYNQVPLQPKDKNSHSVGLDLFLALSKYLKNKDPSKDDRLKQILLKKLQDINDALEIHGGPFLDGEKMTLPDCNLLPKLYHTAVCGKVIKEKAFEIPHNLENIHRYLDAFKASDVFDETKVEEEEIEYGWRKHRML</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BLH8</accession>
    <name>A0A9Q1BLH8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_30994</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BLH8"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8028685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="102" mass="11713" checksum="F6405E56D5ED20F6" modified="2023-09-13" version="1">MFTIKHGVTRRPVRNTGLNPSTSEWKVPETSNFNEAFLELSRSENCLRYVPITTTVCSRTPSLVVIRSWNQFLRYSTLQVYYRTIIDVHALPALGPDQANIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BM76</accession>
    <name>A0A9Q1BM76_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NLR family CARD domain-containing protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_28608</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BM76"/>
    <dbReference type="OrthoDB" id="427518at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312">
        <property type="entry name" value="NACHT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312:SF2">
        <property type="entry name" value="NUCLEOTIDE-BINDING OLIGOMERIZATION DOMAIN-CONTAINING PROTEIN 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NACHT" evidence="1">
        <location>
            <begin position="243"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8029257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="748" mass="87276" checksum="EF32A4FEBD936BC2" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BNF0</accession>
    <name>A0A9Q1BNF0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_29089</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BNF0"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8029644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="78" mass="9329" checksum="5B07E26F211F9CA0" modified="2023-09-13" version="1">MELTYHLEGEENSKRLQSLSKWKQTKRFMLDLGLVFVWMVTTEKIVSGVPLCHRRGQLHWRCAKVLKRLCMEFHNLGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BNY1</accession>
    <name>A0A9Q1BNY1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_26497</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BNY1"/>
    <dbReference type="OrthoDB" id="6361178at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8030167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="88" mass="9728" checksum="622E7349C26910A5" modified="2023-09-13" version="1">MRYGKRKNPIIFGGGERSSGVTGSQTLKTSLTRYLKMRYGKRKNPIIFGGGERSSGVTGGQILKTSLTRYLKKGSSDSSQTWYIDAPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BPL0</accession>
    <name>A0A9Q1BPL0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Rhomboid-related protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_27174</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S54 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BPL0"/>
    <dbReference type="OrthoDB" id="10257275at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1540.10:FF:000008">
        <property type="entry name" value="RHOMBOID-like protein 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1540.10">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022764">
        <property type="entry name" value="Peptidase_S54_rhomboid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43066:SF1">
        <property type="entry name" value="RHOMBOID PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43066">
        <property type="entry name" value="RHOMBOID-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01694">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="119"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="152"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S54 rhomboid" evidence="10">
        <location>
            <begin position="81"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="265"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="274"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="294"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="309"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="327"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="358"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8030693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="372" mass="42307" checksum="9C2ECADB8763E98D" modified="2023-09-13" version="1">MFRGRGRHRGYGRRREGMALALLAMQIYQVGIRHIPPTTLATVALNVAIYLRALNSFFKSFIRHPSITNICVSTSQVLYKRDWPRLFLAAWFHLDEWHLYYNMASFLWKGIQLERRLGTIYFAYLIAVFSVLTNLVMVGVNIAAAELLDDGSYIHTCAAGFSGVIFALKVLSTHYSPRDSQRIMGIINVPSRWACWIELVVIQLIVPRASFTGHLAGILVGLAYIKGPLKNIMDIGSTAISSMTDGFHRTDAHYREEARSYRYQAGTSGYNNRGTGGYNDGHGGLSEEEQIQEALRRSRETAYAERNGYPNQPGNYHQQAEGQQDGRTYDRLYPDLSDRQENMGAPYPEEDAMPSPHSQDELRRRRLARFDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BPR4</accession>
    <name>A0A9Q1BPR4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Soluble interferon alpha/beta receptor OPG204</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HOLleu_26985</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Nanhai2018</strain>
            <tissue evidence="17">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Counteracts the antiviral effects of host IFN-alpha/beta and key IFN-inducible proteins involved in viral RNA degradation suxh as host OAS1. Acts as a soluble IFN-alpha receptor and thus inhibits the interaction between host IFN-alpha and its receptor.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Interacts with host IFNA1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the interleukin-1 receptor family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BPR4"/>
    <dbReference type="OrthoDB" id="1421090at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039502">
        <property type="term" value="P:symbiont-mediated suppression of host type I interferon-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015621">
        <property type="entry name" value="IL-1_rcpt_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11890">
        <property type="entry name" value="INTERLEUKIN-1 RECEPTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11890:SF44">
        <property type="entry name" value="X-LINKED INTERLEUKIN-1 RECEPTOR ACCESSORY PROTEIN-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13676">
        <property type="entry name" value="TIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01537">
        <property type="entry name" value="INTRLKN1R1F"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0244">Early protein</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0945">Host-virus interaction</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="3" id="KW-1090">Inhibition of host innate immune response by virus</keyword>
    <keyword evidence="5" id="KW-1114">Inhibition of host interferon signaling pathway by virus</keyword>
    <keyword evidence="9" id="KW-0922">Interferon antiviral system evasion</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="17" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0899">Viral immunoevasion</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040375354" description="Soluble interferon alpha/beta receptor OPG204" evidence="14">
        <location>
            <begin position="23"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="16">
        <location>
            <begin position="32"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="TIR" evidence="15">
        <location>
            <begin position="292"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00041012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ8030535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="477" mass="54749" checksum="AD05D1B62B41CDC8" modified="2023-09-13" version="1">MKIAVCFPCILLIVCRLPSAQSVPCSSYADKPFFERNTSSLIFAEEGDTALLNFCSAANYCSIQWFKNGQRLDPSQNFTDGHLVVKDDGRMLWFSPVLASDKGNYTCVATNNAGSVRKAQELEVKRSNKCPAFTTESHKALDRLTEVGADHFEKWEIETNMDLDCYFFKEIRAGNSSNDTDCSDCGWCYKDTVTPDIWYAVFNLTHISQKDFATYKFFVYDKHRTCTRLSVEINLSQNKTSFSEVNSTTVTTATCSIVLFVMLCTLTWHVIKVDVQLFVKDHLYKTEDDDGRCYDAYISYTWTDTDRLFVLQLKSRLEKKGYRVYVPEIDNLPGGCTAHQLTQALQRSRRFIMVLSPEYVRANFTAFEVNIFIEAVTQYKNFIIPIEFQGLLSPDESAILRHVLKVGQTVTWANIDWPLSDNSVYHEEESLDTIIVENSKNSKTVMEKSFRMKGFKQLVLKLPPKPKRTTRNFYLLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BPT0</accession>
    <name>A0A9Q1BPT0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Cell division cycle protein 20-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_27339</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat CDC20/Fizzy family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BPT0"/>
    <dbReference type="OrthoDB" id="10263272at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010997">
        <property type="term" value="F:anaphase-promoting complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990757">
        <property type="term" value="F:ubiquitin ligase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905786">
        <property type="term" value="P:positive regulation of anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033010">
        <property type="entry name" value="Cdc20/Fizzy"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056150">
        <property type="entry name" value="WD40_CDC20-Fz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19918">
        <property type="entry name" value="CELL DIVISION CYCLE 20 CDC20 FIZZY -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19918:SF8">
        <property type="entry name" value="FI02843P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24807">
        <property type="entry name" value="WD40_CDC20-Fz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3 10" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0498">Mitosis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 7" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="CDC20/Fizzy WD40" evidence="9">
        <location>
            <begin position="208"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="254"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="337"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="470"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="55"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="55"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF24807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8030813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="532" mass="59124" checksum="6D6C56ECB054854E" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BR52</accession>
    <name>A0A9Q1BR52_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_27982</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8031297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BR52"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8031297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="55" mass="6130" checksum="B99183EE64DA9DE3" modified="2023-09-13" version="1">MATYSHFVVIAGILGLAHAAFSAAQRNFMIKLFSLHITPKDVIIRLFCSHSLHAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BS99</accession>
    <name>A0A9Q1BS99_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_25026</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BS99"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8031730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="111" mass="12204" checksum="9F3FA49515741C3D" modified="2023-09-13" version="1">MGFLATVAALGAIVGPYIPGITIVTAPAMTLWGPIAAVAGIVATFTGGLGLWINDKIERQAEIERVERDVFRRNVIVGVIFIFVVIAVILFIWRRRRGAGGFRLRIPFIRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BUH7</accession>
    <name>A0A9Q1BUH7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_22988</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8032903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BUH7"/>
    <dbReference type="OrthoDB" id="10064731at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000032">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 297-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon gypsy-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054465">
        <property type="entry name" value="Integrase_p58-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF13">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22938">
        <property type="entry name" value="Integrase_p58_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="2">
        <location>
            <begin position="856"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="325"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="589"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="689"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="325"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="340"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="364"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="589"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="633"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="658"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="705"/>
            <end position="729"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8032903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1274" mass="145032" checksum="510B9ADA630D90FE" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BVM7</accession>
    <name>A0A9Q1BVM7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Choline dehydrogenase</fullName>
            <ecNumber evidence="8">1.1.99.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_24022</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>choline + A = betaine aldehyde + AH2</text>
            <dbReference type="Rhea" id="RHEA:17433"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15354"/>
            <dbReference type="ChEBI" id="CHEBI:15710"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="EC" id="1.1.99.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="8">Amine and polyamine biosynthesis; betaine biosynthesis via choline pathway; betaine aldehyde from choline (cytochrome c reductase route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the GMC oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.99.1" evidence="8"/>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BVM7"/>
    <dbReference type="OrthoDB" id="269227at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008812">
        <property type="term" value="F:choline dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019285">
        <property type="term" value="P:glycine betaine biosynthetic process from choline"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.560.10">
        <property type="entry name" value="Glucose Oxidase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011533">
        <property type="entry name" value="BetA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012132">
        <property type="entry name" value="GMC_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000172">
        <property type="entry name" value="GMC_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007867">
        <property type="entry name" value="GMC_OxRtase_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01810">
        <property type="entry name" value="betA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002550">
        <property type="entry name" value="PRK02106.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552:SF147">
        <property type="entry name" value="CHOLINE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552">
        <property type="entry name" value="GLUCOSE-METHANOL-CHOLINE GMC OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05199">
        <property type="entry name" value="GMC_oxred_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00732">
        <property type="entry name" value="GMC_oxred_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000137">
        <property type="entry name" value="Alcohol_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00623">
        <property type="entry name" value="GMC_OXRED_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00624">
        <property type="entry name" value="GMC_OXRED_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6 7" id="KW-0274">FAD</keyword>
    <keyword evidence="3 7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucose-methanol-choline oxidoreductase N-terminal" evidence="9">
        <location>
            <begin position="121"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Glucose-methanol-choline oxidoreductase N-terminal" evidence="10">
        <location>
            <begin position="293"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000137-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS00623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS00624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8033692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="592" mass="65620" checksum="EFD416A0CED341C1" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BWI6</accession>
    <name>A0A9Q1BWI6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Bcl-2 Bcl-2 homology region 1-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_24291</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Bcl-2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BWI6"/>
    <dbReference type="OrthoDB" id="6021377at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051400">
        <property type="term" value="F:BH domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097192">
        <property type="term" value="P:extrinsic apoptotic signaling pathway in absence of ligand"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008630">
        <property type="term" value="P:intrinsic apoptotic signaling pathway in response to DNA damage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001836">
        <property type="term" value="P:release of cytochrome c from mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.437.10">
        <property type="entry name" value="Blc2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036834">
        <property type="entry name" value="Bcl-2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046371">
        <property type="entry name" value="Bcl-2_BH1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026298">
        <property type="entry name" value="Bcl-2_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002475">
        <property type="entry name" value="Bcl2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11256">
        <property type="entry name" value="BCL-2 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11256:SF47">
        <property type="entry name" value="BCL-2-LIKE PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00452">
        <property type="entry name" value="Bcl-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56854">
        <property type="entry name" value="Bcl-2 inhibitors of programmed cell death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50062">
        <property type="entry name" value="BCL2_FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Bcl-2 Bcl-2 homology region 1-3" evidence="7">
        <location>
            <begin position="37"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8033909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="107" mass="12314" checksum="7D890CB92E475CAF" modified="2023-09-13" version="1">MAYTSHGKIARELGEDYVRYKLGFDTDTSPSVYAETLRRAGDQVEDRYSLALQWMVSQLNYDPLLDAERSLRVIFDAICENEESSWGRIVMVYVFAARLAKYCQTQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-02-05" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BXL0</accession>
    <name>A0A9Q1BXL0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Intersectin-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_21295</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="427518at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01120">
        <property type="entry name" value="RecA-like_superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SH3_CD2AP-like_1"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="10"/>
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    </dbReference>
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        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
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        <property type="entry name" value="CIN85 AND CD2AP RELATED, ISOFORM J"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="10"/>
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    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="NACHT" evidence="6">
        <location>
            <begin position="287"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="5">
        <location>
            <begin position="1006"/>
            <end position="1065"/>
        </location>
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        <location>
            <begin position="1095"/>
            <end position="1154"/>
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        <location>
            <begin position="1165"/>
            <end position="1224"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="5">
        <location>
            <begin position="1232"/>
            <end position="1291"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="5">
        <location>
            <begin position="1302"/>
            <end position="1361"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="5">
        <location>
            <begin position="1369"/>
            <end position="1428"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="5">
        <location>
            <begin position="1441"/>
            <end position="1500"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="5">
        <location>
            <begin position="1508"/>
            <end position="1567"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="5">
        <location>
            <begin position="1578"/>
            <end position="1637"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="5">
        <location>
            <begin position="1645"/>
            <end position="1704"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="670"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="185"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="692"/>
            <end position="717"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8034449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1724" mass="193840" checksum="CB766E65BB3D1018" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BXV9</accession>
    <name>A0A9Q1BXV9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SGNH hydrolase-type esterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_21739</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BXV9"/>
    <dbReference type="OrthoDB" id="10172666at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="CDD" id="cd00229">
        <property type="entry name" value="SGNH_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12690">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="52"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8034765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="289" mass="32705" checksum="161D6B55A3B7B421" modified="2023-09-13" version="1">MQLNINNTHPPTRRGPHMVEYGVPISNSFANLRDEFVATDDHDVDNDNDFISTMPPSKPPKTTKMSRPSETNDVFPDHLCSVSTPSDVPPKLPDTPSIFVQRRPVAVIVGDSIPRYLVGRRLSRRYRVVNHCIPGMTLQKLITFMPILIRDDNPSIVIVHCGTNNVLTHSTQKMLDLFSQLDYNIKALVPGVQVAFSGLVGNRICPQTDILICHINACIQRFCEDHNSSFIDNCNIPLSYLSRDGIHLNRWGIKQLAMNFISYLRFRPILEDQLGNFQMESHPNSVRTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BXY7</accession>
    <name>A0A9Q1BXY7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HOLleu_21602</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Nanhai2018</strain>
            <tissue evidence="15">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:17990"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:46609"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BXY7"/>
    <dbReference type="OrthoDB" id="1405469at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004694">
        <property type="term" value="F:eukaryotic translation initiation factor 2alpha kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050339">
        <property type="entry name" value="CC_SR_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042:SF160">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE EIF2-ALPHA KINASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="15" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-0652">Protein synthesis inhibitor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 12" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="15"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="88"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="392"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="94"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="109"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="403"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ8034670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="458" mass="52091" checksum="B990FB4342B11AF2" modified="2023-09-13" version="1">MEESMLHCGRYERDFEEILKIGKGGGGKVIKVRHRLDNQIYAVKKIKIKSTDNLSIEQICREVKTLAEVSNSSCPSIVRYHTAWFESTDEADSEPSSQESVSTSEDDSPVQTNWSNGSGELKFSPLEPTPKIVEMVHSNDSQDEIVFEGSQDADNASQMRYMLYIQMELCGQPLSEWLEGRNKDGQPAVSKKQNLDILRQLLEGLHSLHQKDIIHRDLKPSNIFVTSHEPHVKIGDFGFSRKIICREEKDDELSSDVGTALYMAPEVKDGNDYDTKADMFSLGILLCELFSPFTTECERCMTILQLRETQCVTLEFRERWPDMADLIEQLIREDTTQRPNASQLLDIPEHKQLRPGDVVIERLTSQIENLTSKFKQLKGSVQKQFLASARAYNDGGPADCGDTADKKYVDDKDYGSYDKKEDNKNSEGKDDTDLKDIEDKENHVGHEDNEFIEIDEMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BYS0</accession>
    <name>A0A9Q1BYS0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_22069</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BYS0"/>
    <dbReference type="OrthoDB" id="8945351at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46601:SF1">
        <property type="entry name" value="ADF-H DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46601">
        <property type="entry name" value="ULP_PROTEASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8034999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="275" mass="30908" checksum="16C3E384EC284FC6" modified="2023-09-13" version="1">MNDVQHKLPFKISQVHYWSDGAGSQFKNKYIFANLLHHEEQFGFNADWSFFATAHGKGPIDGIGGQVKRCVWRAVLQNRIVVNTAEEFYSAAKNLCRKITVLYVDKGVIERTAESLQELWSRTITIPGTLHLHYLRPSGKPGHIEHGRHSPFRAGEGDLQLARVQSINVNDSLAGGQQRNAVIEPQQSPNQERQPGAILPAVQPTSETYHHGDFLLGGLSTGRSRKVFLSEVVALDNENQGELQISFLRAADTDKQLFVFPHREDISWFSRSEVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BYW5</accession>
    <name>A0A9Q1BYW5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_22263</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BYW5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8035135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="245" mass="28243" checksum="3B951ACC49F9D550" modified="2023-09-13" version="1">MIDLNEIKSIILQGIQDGYYPYDMTAIADRLFVCVGDRKEISERKSLIFEEKNGRILSELTKPTDTARWYVHSVGVNMNTLGIAGVVWVDSYYYKEGKYRQIVFYSLLSEKNCSFLIVEVESGVSRIRISDRGDRVITGNLRTGEVKKYDMAELFTFSHFKEKLTSTLQTNECIKLANFFNIPKDQTDAIMSSHKPSEHLLLALEANSTLQPNNVDRLIEAFDELRTNPCIRHVTEIFRKTKCKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C513</accession>
    <name>A0A9Q1C513_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_15744</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C513"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8038348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="60" mass="7044" checksum="08FB318E85BF77AF" modified="2023-09-13" version="1">MPYGAVCFYGCVLRVLLSIIASLVVTRSWNQFLRYSTLQVYYCTIVQRGMHYHPWTRPGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C624</accession>
    <name>A0A9Q1C624_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_17022</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C624"/>
    <dbReference type="OrthoDB" id="9998845at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8039341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="104" mass="11285" checksum="DCDF2F4BDF94FF95" modified="2023-09-13" version="1">MVLPIHGRTMIDINATVYQYRPIISKLLAAHGLTGCDKVATHFGIGKAAALLILRTGAHTLANLDDTNCDLSKVIYQATPFVLACYGQDGCTSMTEARKEMWTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C634</accession>
    <name>A0A9Q1C634_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase A2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_16642</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C634"/>
    <dbReference type="OrthoDB" id="10055784at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331:SF5">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8039052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="442" mass="49555" checksum="98EF9985BBCAD5F3" modified="2023-09-13" version="1">MKSRIRATAPLRSEASKSGAAKKFHKCCLCESTNHWTDQRQTFLNLTPAQQWQHVKEHHGCFSCLKKAGRDHRMSNCRRKRACSEVVNGTQCGLFHHPLLHLPAGSVSGIVGVALNAKDEALLPVVEVDIEGPRGIKQRGNILLDSGAQISLIRTSLAEQLNLKGADADKKYQLKIRPLTKSGVYSISAVALPCISKELKYMDVDKTSKLFGLKPDDVRRGNRSVDILIGIDYAHLHYGDIRQARHLVARNSPLGWLIFGSSATQEVVASSVLHVKLSTPVDLKDFWTSESMGVNPSSCECKPNISSKLEADEGKIISDSCKKKLVHSGLSHIHGKRMLSFCPITGHRQKEPSESLKKRLSKNPEYAEAYKREMMETVEMKFARKLTEKDIKQYKGPVHYISHHAVIIPDKRRTPAAQNKSYSIFHWLPYFHSNIKSMSYSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C8J4</accession>
    <name>A0A9Q1C8J4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Metalloprotease TIKI homolog</fullName>
            <ecNumber evidence="13">3.4.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HOLleu_14306</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Nanhai2018</strain>
            <tissue evidence="15">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Metalloprotease that acts as a negative regulator of the Wnt signaling pathway.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="13">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <text evidence="13">Divalent metal cations. Mn(2+) or Co(2+).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Cell membrane</location>
            <topology evidence="13">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the TIKI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.-.-" evidence="13"/>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C8J4"/>
    <dbReference type="OrthoDB" id="10040378at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030178">
        <property type="term" value="P:negative regulation of Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14789">
        <property type="entry name" value="Tiki"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040230">
        <property type="entry name" value="TIKI1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002816">
        <property type="entry name" value="TraB/PrgY/GumN_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31120">
        <property type="entry name" value="METALLOPROTEASE TIKI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31120:SF6">
        <property type="entry name" value="METALLOPROTEASE TIKI HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01963">
        <property type="entry name" value="TraB_PrgY_gumN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 13 15" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4 13" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 13" id="KW-0732">Signal</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="13" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="439"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="365"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="365"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU369069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ8040104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="463" mass="52943" checksum="8352A32D69BD3EEB" modified="2023-09-13" version="1">MRRLGLDIAVFVISFAFQVFSAYIPKVSVVELRQRNHCFPALTNSSKMTNSYLWRIVRDPPVYFFGTIHVPYTRVWDYIPENAKQAFRESDHAFFELDLTDVRTTHTLRRCQMLPRGENLSSVLPERMYKRLKNHLGYVEQMMPTWGVYDEGLFHAIAGNWERKRPVWVMLMVNTLNEADMKSRGIPVLDYYLTLEAKRMDKGVGAVEYVQEQCIPFNGLNVSQVLFLLNRTLWQQEALREGTVQNSFTTDDLIWHYNCGDLNSMIFSLDTAQIPSVLNSTMSQAEIALAQEIDRYFVNELIDKRNQNMASRIKEIVDSNKKESFFFAFGAGHFLGNNSVLDLLRAEGFVIENIPADVDLDSLTSNSISDTGQGPNEHRSTQQQWDTAERNSHFNELWVHIDEVESEKPVYVNEALQRITTTVPCNHNGAVSGLHNHPLGSSTFLLLLLSIVTAMLLAGTRST</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CC51</accession>
    <name>A0A9Q1CC51_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Alanine--glyoxylate aminotransferase 2, mitochondrial</fullName>
            <ecNumber evidence="28">2.6.1.18</ecNumber>
            <ecNumber evidence="12">2.6.1.40</ecNumber>
            <ecNumber evidence="5">2.6.1.44</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">(R)-3-amino-2-methylpropionate--pyruvate transaminase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Beta-ALAAT II</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Beta-alanine-pyruvate aminotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="30">D-3-aminoisobutyrate-pyruvate aminotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">D-AIBAT</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="29">D-beta-aminoisobutyrate-pyruvate aminotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="40" type="ORF">HOLleu_13225</name>
    </gene>
    <organism evidence="40 41">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="40" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="40">Nanhai2018</strain>
            <tissue evidence="40">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="38">Multifunctional aminotransferase with a broad substrate specificity. Catalyzes the conversion of glyoxylate to glycine using alanine as the amino donor. Catalyzes metabolism of not L- but the D-isomer of D-beta-aminoisobutyric acid to generate 2-methyl-3-oxopropanoate and alanine. Catalyzes the transfer of the amino group from beta-alanine to pyruvate to yield L-alanine and 3-oxopropanoate. Can metabolize NG-monomethyl-L-arginine (NMMA), asymmetric NG,NG-dimethyl-L-arginine (ADMA) and symmetric NG,N'G-dimethyl-L-arginine (SDMA). ADMA is a potent inhibitor of nitric-oxide (NO) synthase, and this activity provides mechanism through which the kidney regulates blood pressure.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>(2S)-2-aminobutanoate + glyoxylate = 2-oxobutanoate + glycine</text>
            <dbReference type="Rhea" id="RHEA:77339"/>
            <dbReference type="ChEBI" id="CHEBI:16763"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:74359"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>(R)-3-amino-2-methylpropanoate + pyruvate = 2-methyl-3-oxopropanoate + L-alanine</text>
            <dbReference type="Rhea" id="RHEA:18393"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:57700"/>
            <dbReference type="ChEBI" id="CHEBI:57731"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="EC" id="2.6.1.40"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:18394"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>2-oxobutanoate + L-alanine = (2S)-2-aminobutanoate + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:77355"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:16763"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:74359"/>
            <dbReference type="EC" id="2.6.1.44"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="33">
            <text>2-oxohexanoate + N(omega),N(omega)-dimethyl-L-arginine = L-2-aminohexanoate + 5-(3,3-dimethylguanidino)-2-oxopentanoate</text>
            <dbReference type="Rhea" id="RHEA:77363"/>
            <dbReference type="ChEBI" id="CHEBI:35177"/>
            <dbReference type="ChEBI" id="CHEBI:58326"/>
            <dbReference type="ChEBI" id="CHEBI:58455"/>
            <dbReference type="ChEBI" id="CHEBI:197301"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>2-oxopentanoate + N(omega),N(omega)-dimethyl-L-arginine = 5-(3,3-dimethylguanidino)-2-oxopentanoate + L-2-aminopentanoate</text>
            <dbReference type="Rhea" id="RHEA:77359"/>
            <dbReference type="ChEBI" id="CHEBI:28644"/>
            <dbReference type="ChEBI" id="CHEBI:58326"/>
            <dbReference type="ChEBI" id="CHEBI:58441"/>
            <dbReference type="ChEBI" id="CHEBI:197301"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>3-oxopropanoate + L-alanine = beta-alanine + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:14077"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:33190"/>
            <dbReference type="ChEBI" id="CHEBI:57966"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="EC" id="2.6.1.18"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="26">
            <dbReference type="Rhea" id="RHEA:14079"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="32">
            <text>L-ornithine + glyoxylate = 5-amino-2-oxopentanoate + glycine</text>
            <dbReference type="Rhea" id="RHEA:77331"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:58802"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>L-ornithine + pyruvate = 5-amino-2-oxopentanoate + L-alanine</text>
            <dbReference type="Rhea" id="RHEA:77327"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:58802"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="37">
            <text>N(omega),N('omega)-dimethyl-L-arginine + glyoxylate = 5-(3,3'-dimethylguanidino)-2-oxopentanoate + glycine</text>
            <dbReference type="Rhea" id="RHEA:77315"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:197308"/>
            <dbReference type="ChEBI" id="CHEBI:197310"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>N(omega),N('omega)-dimethyl-L-arginine + pyruvate = 5-(3,3'-dimethylguanidino)-2-oxopentanoate + L-alanine</text>
            <dbReference type="Rhea" id="RHEA:77307"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:197308"/>
            <dbReference type="ChEBI" id="CHEBI:197310"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="34">
            <text>N(omega),N(omega)-dimethyl-L-arginine + 2-oxobutanoate = 5-(3,3-dimethylguanidino)-2-oxopentanoate + (2S)-2-aminobutanoate</text>
            <dbReference type="Rhea" id="RHEA:77351"/>
            <dbReference type="ChEBI" id="CHEBI:16763"/>
            <dbReference type="ChEBI" id="CHEBI:58326"/>
            <dbReference type="ChEBI" id="CHEBI:74359"/>
            <dbReference type="ChEBI" id="CHEBI:197301"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="31">
            <text>N(omega),N(omega)-dimethyl-L-arginine + glyoxylate = 5-(3,3-dimethylguanidino)-2-oxopentanoate + glycine</text>
            <dbReference type="Rhea" id="RHEA:77311"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:58326"/>
            <dbReference type="ChEBI" id="CHEBI:197301"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>N(omega),N(omega)-dimethyl-L-arginine + oxaloacetate = 5-(3,3-dimethylguanidino)-2-oxopentanoate + L-aspartate</text>
            <dbReference type="Rhea" id="RHEA:77343"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:58326"/>
            <dbReference type="ChEBI" id="CHEBI:197301"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>N(omega),N(omega)-dimethyl-L-arginine + pyruvate = 5-(3,3-dimethylguanidino)-2-oxopentanoate + L-alanine</text>
            <dbReference type="Rhea" id="RHEA:77303"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:58326"/>
            <dbReference type="ChEBI" id="CHEBI:197301"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="35">
            <text>N(omega)-methyl-L-arginine + glyoxylate = 5-(3-methylguanidino)-2-oxopentanoate + glycine</text>
            <dbReference type="Rhea" id="RHEA:77323"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:114953"/>
            <dbReference type="ChEBI" id="CHEBI:197314"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>N(omega)-methyl-L-arginine + pyruvate = 5-(3-methylguanidino)-2-oxopentanoate + L-alanine</text>
            <dbReference type="Rhea" id="RHEA:77319"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:114953"/>
            <dbReference type="ChEBI" id="CHEBI:197314"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>glyoxylate + L-alanine = glycine + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:24248"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="EC" id="2.6.1.44"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:24249"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="36">
            <text>oxaloacetate + L-alanine = L-aspartate + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:77347"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 39">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="40">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.18" evidence="28"/>
    <dbReference type="EC" id="2.6.1.40" evidence="12"/>
    <dbReference type="EC" id="2.6.1.44" evidence="5"/>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8042220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CC51"/>
    <dbReference type="OrthoDB" id="10261433at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047305">
        <property type="term" value="F:(R)-3-amino-2-methylpropionate-pyruvate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008453">
        <property type="term" value="F:alanine-glyoxylate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016223">
        <property type="term" value="F:beta-alanine:pyruvate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009436">
        <property type="term" value="P:glyoxylate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019481">
        <property type="term" value="P:L-alanine catabolic process, by transamination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000055">
        <property type="entry name" value="Alanine--glyoxylate aminotransferase 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049704">
        <property type="entry name" value="Aminotrans_3_PPA_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45688">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45688:SF3">
        <property type="entry name" value="ALANINE--GLYOXYLATE AMINOTRANSFERASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000521">
        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 40" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8 39" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="41" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00039130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00039862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00041662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00041845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00042611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00042669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00043669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00043679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00043726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00043749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00043751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00043758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00043777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00043798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00043825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00043826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00044055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00044257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00044258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00047892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00048264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="ARBA" id="ARBA00048500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="ARBA" id="ARBA00048560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="35">
        <source>
            <dbReference type="ARBA" id="ARBA00048760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="36">
        <source>
            <dbReference type="ARBA" id="ARBA00048916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="37">
        <source>
            <dbReference type="ARBA" id="ARBA00049480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="38">
        <source>
            <dbReference type="ARBA" id="ARBA00058068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="39">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="40">
        <source>
            <dbReference type="EMBL" id="KAJ8042220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="41">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="509" mass="56520" checksum="C2219BB03303682C" modified="2023-09-13" version="1">MALLRCSKAWRKISFSLPKQPTVSNFVRSKSAVPEMSTDNGTTPSMPPCDFKPGPLVGPSLDDVKQIRKEKLSAGLMTYYRTPVYIHQGHMQWLFDNEGKRYLDMFAGIVTVSVGHCHPKVNKALHDQVDRLWHTTNIYLHPNIHEYAEKLTSKLPGNLKVCLFTNSGSEANDLALHLARLYTGSYDVISFRNAYHGMSPYLMGLTALSTWHYQVPRGFGIHNTMNPDVYRGPWGGKNCRDSPVQTNRDCNCQAGQCEANDMYLDQFNDLLKHSLPKRIAGFFAESMQGVGGAVQYPRGFLKDAFEMVRSKGGVCIADEVQTGFGRLGSHYWGFESHDVMPDMVVIAKGMGNGFPLGAVITTPEIGHCLSQALHFNTYGGNPMACAVGSAVLDAIEEDGLQQNCEEVGTYFLLEFAKMREEFECIGDVRGKGLMVGVEMVTDKKSRTPLPAADMADIWEETKQMGVLFGKGGLHGNVFRIKPPMCITKDDATFALAVFREALKRYLAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CED3</accession>
    <name>A0A9Q1CED3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_10157</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CED3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="107"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8043178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="141" mass="15943" checksum="68B53F1BF98F131D" modified="2023-09-13" version="1">MPVVKTLDLNDTLETVFDCLNDTKETVFEETWSDCSSEIEDEHLIRDNPIGIFQLSSENLLEDDHVSTDEQRYSPISLSPKKRSRKSISGSLSSLLSDEVTFTNNTLHKDVRHGENISSTADEGASEMQEEDDQLFLYLSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CET4</accession>
    <name>A0A9Q1CET4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">G patch domain-containing protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_11347</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8044012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CET4"/>
    <dbReference type="OrthoDB" id="5842926at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039536">
        <property type="term" value="P:negative regulation of RIG-I signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032480">
        <property type="term" value="P:negative regulation of type I interferon production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040341">
        <property type="entry name" value="GPATCH3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14390">
        <property type="entry name" value="G PATCH DOMAIN CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14390:SF2">
        <property type="entry name" value="G PATCH DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="296"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="305"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8044012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="453" mass="51592" checksum="7B295873963839F4" modified="2023-09-13" version="1">MISNISLFVSFSRSSKLFLTFVEEKRFKCFHFRNRPEYRLSRCPSKASSIDHRTISDNDVNVSNVTNLNVIGSGTSHRIGPTDVLARPNVPSATTSPPRKCCIIKLSRKNFQKLVHLYNKKPWIDQNGDAMRLRCNIVSVNIKNSSNSEDAVEDIVQEVVEESKERDVGISGGLWEGERLRGVHGNRAGSISFSEDDVNSLPELNPPDIMPRGNVGTPTQTFLELIKDCKIPPSVIRKLGLIFPKRRSNRQYGNVPFHYGTNVTPGREEEGVALTGKGEEIIDTVVNISLNVKKNVQGTESQETSSHHQIREESPERNVQSESDDDDDDTCEEWERYEALHDDVTKQDRTEERLFEEEMEIVWDKGSSGLVFYTDAAYWDAEKGDFDERTSDDWDVDMSIFYENGSGTKDAKDWLQMQKEMRLRRGEEDTSIFTKNQIGKFEKHTKVCGTRSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CF73</accession>
    <name>A0A9Q1CF73_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="18">P2X purinoceptor 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HOLleu_10460</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Nanhai2018</strain>
            <tissue evidence="18">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the P2X receptor family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CF73"/>
    <dbReference type="OrthoDB" id="494673at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098794">
        <property type="term" value="C:postsynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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<entry dataset="TrEMBL" created="2023-09-13" modified="2025-04-02" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CFS3</accession>
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        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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</entry>
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    <accession>A0A9Q1CJ22</accession>
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</entry>
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        <name type="scientific">Holothuria leucospilota</name>
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            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CJ75"/>
    <dbReference type="OrthoDB" id="5975154at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005230">
        <property type="term" value="F:extracellular ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0675">Receptor</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040264632" evidence="1">
        <location>
            <begin position="22"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="2">
        <location>
            <begin position="27"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8045454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="274" mass="30201" checksum="C9F08B1F3E75B7EC" modified="2023-09-13" version="1">MTSCWMLSGLLILSMSGLSAANVEGETRLKGLLFSHYDKTVAPETPLVLTFSTALKKLVDANPDKGLVTVDLFLSQSWQDSRLAWDPQEFNVTDQSIIVPREWVFLPDMKLQNGFVTELLADPEVLIYSNGGAYYVPYVRAEVYCDLPSKPFEGLICDVRIGPWSRTSNRITIESFTVDDSAVQLGGQCTLGKSEAKIVRQLFPCCPETYEAAILSLNVSCQAPESSFPVAKVNVTCELVNPAPNSAVESHYLFPYFFVAPIYIVLHSLTGLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CJV6</accession>
    <name>A0A9Q1CJV6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Trafficking protein particle complex subunit 6B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_09199</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">cis-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAPP small subunits family. BET3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8046040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CJV6"/>
    <dbReference type="OrthoDB" id="941624at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005801">
        <property type="term" value="C:cis-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030008">
        <property type="term" value="C:TRAPP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14944">
        <property type="entry name" value="TRAPPC6A_Trs33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1380.20">
        <property type="entry name" value="Trafficking protein particle complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024096">
        <property type="entry name" value="NO_sig/Golgi_transp_ligand-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007194">
        <property type="entry name" value="TRAPP_component"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037992">
        <property type="entry name" value="TRAPPC6/Trs33"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12817:SF0">
        <property type="entry name" value="GEO08327P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12817">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04051">
        <property type="entry name" value="TRAPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111126">
        <property type="entry name" value="Ligand-binding domain in the NO signalling and Golgi transport"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8046040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="131" mass="14675" checksum="A027AA898DA78091" modified="2023-09-13" version="1">MTNNIISKLEQMGCRVGQSLAERFTRDSPRFGSELDIIKFICKEFWVGTFKKQVDNLRTNHLGVYVILDSRFKLLTHMSAGEQYLESAPKYLAFTCGLVRGALASFGINSVVTAEVSTMPSCKFQISIPKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CL02</accession>
    <name>A0A9Q1CL02_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_06104</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CL02"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8047178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="77" mass="9177" checksum="977CEFC714E40867" modified="2023-09-13" version="1">MQMMEEAFPLWSVLEKETKTTLFRIFKSVFLFCGCDLTWKERNTSCTTARRRCLHLCLRATVRAGTEWRLRTGMLRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CMF9</accession>
    <name>A0A9Q1CMF9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Resistance to inhibitors of cholinesterase protein 3 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_00120</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ric-3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8047981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CMF9"/>
    <dbReference type="OrthoDB" id="10070774at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034394">
        <property type="term" value="P:protein localization to cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007271">
        <property type="term" value="P:synaptic transmission, cholinergic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026160">
        <property type="entry name" value="Ric3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032763">
        <property type="entry name" value="RIC3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21723:SF3">
        <property type="entry name" value="PROTEIN RIC-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21723">
        <property type="entry name" value="RESISTANCE TO INHIBITORS OF CHOLINESTERASE PROTEIN 3 RIC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15361">
        <property type="entry name" value="RIC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="Resistance to inhibitors of cholinesterase protein 3 N-terminal" evidence="9">
        <location>
            <begin position="15"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF15361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8047981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="70" mass="7730" checksum="92C4E958E1438B6F" modified="2023-09-13" version="1">MRGRMPGGGPPRKSQFSSGNNEAAAYMARQKQEGSSHGMMGTILPVYAVGIFIYFGYVIYKIFLKDKHKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CMQ5</accession>
    <name>A0A9Q1CMQ5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Leucine-rich repeat-containing protein 74A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_06607</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CMQ5"/>
    <dbReference type="OrthoDB" id="120976at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052394">
        <property type="entry name" value="LRR-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24114">
        <property type="entry name" value="LEUCINE RICH REPEAT FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24114:SF50">
        <property type="entry name" value="RNI-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="55"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="169"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="227"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8047573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="703" mass="77658" checksum="B7BC2F2948768802" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CN65</accession>
    <name>A0A9Q1CN65_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="31">Protein deacetylase HDAC6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="32">Tubulin-lysine deacetylase HDAC6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="37" type="ORF">HOLleu_06915</name>
    </gene>
    <organism evidence="37 38">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="37" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="37">Nanhai2018</strain>
            <tissue evidence="37">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="30">
            <text>N(6)-acetyl-L-lysyl-[alpha-tubulin] + H2O = L-lysyl-[alpha-tubulin] + acetate</text>
            <dbReference type="Rhea" id="RHEA:21548"/>
            <dbReference type="Rhea" id="RHEA-COMP:11278"/>
            <dbReference type="Rhea" id="RHEA-COMP:11279"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="30">
            <dbReference type="Rhea" id="RHEA:21549"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>N(6)-acetyl-L-lysyl-[protein] + H2O = L-lysyl-[protein] + acetate</text>
            <dbReference type="Rhea" id="RHEA:58108"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="29">
            <dbReference type="Rhea" id="RHEA:58109"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="8">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell projection</location>
            <location evidence="7">Axon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cell projection</location>
            <location evidence="4">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Cilium basal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
            <location evidence="5">Cytoskeleton</location>
            <location evidence="5">Microtubule organizing center</location>
            <location evidence="5">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Perikaryon</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the histone deacetylase family. HD type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="37">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CN65"/>
    <dbReference type="OrthoDB" id="424012at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000118">
        <property type="term" value="C:histone deacetylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043204">
        <property type="term" value="C:perikaryon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004407">
        <property type="term" value="F:histone deacetylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040029">
        <property type="term" value="P:epigenetic regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051646">
        <property type="term" value="P:mitochondrion localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051129">
        <property type="term" value="P:negative regulation of cellular component organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032886">
        <property type="term" value="P:regulation of microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd10002">
        <property type="entry name" value="HDAC10_HDAC6-dom1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000342">
        <property type="entry name" value="Histone deacetylase 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.800.20:FF:000005">
        <property type="entry name" value="histone deacetylase 6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="HDACs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001607">
        <property type="entry name" value="Znf_UBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625:SF21">
        <property type="entry name" value="HISTONE DEACETYLASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02148">
        <property type="entry name" value="zf-UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00290">
        <property type="entry name" value="ZnF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50271">
        <property type="entry name" value="ZF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="25" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="28" id="KW-0966">Cell projection</keyword>
    <keyword evidence="22" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="34" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="26" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="19" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0488">Methylation</keyword>
    <keyword evidence="27" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="38" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0678">Repressor</keyword>
    <keyword evidence="24" id="KW-0804">Transcription</keyword>
    <keyword evidence="23" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="14" id="KW-0808">Transferase</keyword>
    <keyword evidence="21" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="18" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="20" id="KW-0862">Zinc</keyword>
    <keyword evidence="17 33" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBP-type" evidence="36">
        <location>
            <begin position="1009"/>
            <end position="1108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="35">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="35">
        <location>
            <begin position="455"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="35">
        <location>
            <begin position="903"/>
            <end position="964"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="34">
        <location>
            <begin position="858"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="35">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="35">
        <location>
            <begin position="16"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="35">
        <location>
            <begin position="35"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="35">
        <location>
            <begin position="455"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="35">
        <location>
            <begin position="905"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="35">
        <location>
            <begin position="920"/>
            <end position="946"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00004489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00007738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00049136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00050910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00068733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00082852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="35">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="36">
        <source>
            <dbReference type="PROSITE" id="PS50271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="37">
        <source>
            <dbReference type="EMBL" id="KAJ8047813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="38">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1113" mass="123881" checksum="2C2A780204588440" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CNA5</accession>
    <name>A0A9Q1CNA5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Retrotransposon gag domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_00624</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CNA5"/>
    <dbReference type="OrthoDB" id="7323790at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8048348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="62" mass="7289" checksum="50EEFC8E93191DB8" modified="2023-09-13" version="1">MYESNKFFSRMQQKGETIDQFVTDLRNLSTTCESGDLKVLYEAKLCGVKSDILREECYEKRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CNV0</accession>
    <name>A0A9Q1CNV0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_00591</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CNV0"/>
    <dbReference type="OrthoDB" id="9909555at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21301">
        <property type="entry name" value="REVERSE TRANSCRIPTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21301:SF10">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8048325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="164" mass="18793" checksum="26193DE8FEA83CE7" modified="2023-09-13" version="1">MNTLNYINEAETQLKNEEFYRPLPSDLTETLKKKLHELLQNQNPDIQSAIKDLIPPYSTPSRFFTLPKIHKLKTIFQERFPDVEHTDIIRSTRLNNMDPPGRPIVSGTGTLTEYISAFVDGHLQTILPKLPTLLQDTNDFLRKLNNVDILPKGPMLVTLDVKSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CP29</accession>
    <name>A0A9Q1CP29_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_01471</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CP29"/>
    <dbReference type="OrthoDB" id="7550384at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33480">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33480:SF1">
        <property type="entry name" value="TYR RECOMBINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8048952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="158" mass="18480" checksum="43251249D683CD9D" modified="2023-09-13" version="1">MTFCPFCRTGQSKLPRYLSTFKGHQNEPEVIQWLTTKDPKLKDIRLTKMRNYGNFLHNVEVRKEKKGFLIPVYRPNYDADPDDYLPCDGCFGYDAKHELWRHRCPLGERTSEGTQGPRKRRNYIQEGKMLLPEEGMSNMTKDIMSSLRNDDDGCCSVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CPU3</accession>
    <name>A0A9Q1CPU3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Alpha-2,8-sialyltransferase 8B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_01804</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 29 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CPU3"/>
    <dbReference type="OrthoDB" id="10264956at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003828">
        <property type="term" value="F:alpha-N-acetylneuraminate alpha-2,8-sialyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006491">
        <property type="term" value="P:N-glycan processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009311">
        <property type="term" value="P:oligosaccharide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23963">
        <property type="entry name" value="GT29_ST8SIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1480.20">
        <property type="entry name" value="Glycosyl transferase family 29"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001675">
        <property type="entry name" value="Glyco_trans_29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050943">
        <property type="entry name" value="Glycosyltr_29_Sialyltrsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038578">
        <property type="entry name" value="GT29-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11987">
        <property type="entry name" value="ALPHA-2,8-SIALYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11987:SF53">
        <property type="entry name" value="ALPHA-2,8-SIALYLTRANSFERASE 8F-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00777">
        <property type="entry name" value="Glyco_transf_29"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040112682" evidence="11">
        <location>
            <begin position="27"/>
            <end position="657"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8049181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="657" mass="74648" checksum="D22DE511DB18267D" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CSN8</accession>
    <name>A0A9Q1CSN8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Zinc finger CCHC domain-containing protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_03512</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CSN8"/>
    <dbReference type="OrthoDB" id="7608935at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002218">
        <property type="term" value="P:activation of innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042509">
        <property type="entry name" value="ZCCHC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22639">
        <property type="entry name" value="GAG-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22639:SF3">
        <property type="entry name" value="ZINC FINGER CCHC DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="162"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="198"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="222"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="297"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8050346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="378" mass="41490" checksum="001AEFBEAC6D6BDA" modified="2023-09-13" version="1">MPGGRDSTIVKLQFESEVNVTPPQVLTVLKNNKVEVKEIEMLQALAARNTYDLKFRSDAARMQGVSCLKGLEGLTVTPYDRSVVVTVLYLNFEVEQGLVARVLSQYGTVSDMRWVTYNEGELKGILNGKRQFRMVLKREIPSFLFIGGSKAHIRYFGQPRTCFKCGEEGHEAKSCPNRRCGRCRQVGHDKAECPNQVRCNLCGEEGHVFGACPELYSAHASEDVGQAPEPSQLGPEYSTQELEEVAHEVEQDPLASKQCEAGTVTPEADSPPSLPTEPREAAIMTIEEDLHLSSESVSVMSDDGGDDDGGEPTSEPPPKDPPSGEGWFDLCEGEGSESALKRSASGEVSEWGDRLSERPVPSPRTKKIKSQQELLVCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CT67</accession>
    <name>A0A9Q1CT67_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">DNA transposase THAP9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_03912</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CT67"/>
    <dbReference type="OrthoDB" id="10060099at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.210.20">
        <property type="entry name" value="THAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026516">
        <property type="entry name" value="THAP1/10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006612">
        <property type="entry name" value="THAP_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038441">
        <property type="entry name" value="THAP_Znf_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048365">
        <property type="entry name" value="TNP-like_RNaseH_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46600">
        <property type="entry name" value="THAP DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46600:SF11">
        <property type="entry name" value="THAP DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05485">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21787">
        <property type="entry name" value="TNP-like_RNaseH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00692">
        <property type="entry name" value="DM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00980">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50950">
        <property type="entry name" value="ZF_THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="THAP-type" evidence="7">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="86"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8050631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="375" mass="42944" checksum="E80494CC7B63EF09" modified="2023-09-13" version="1">MPGCCAFGCTNRCSKGFKLYRFPTDPQRRKLWEVKISRVGWKASNSSRLCEVHFDRHQFESGRADQKRKLKNSAVPTIFCHRSILKTRKSPKKRQTEVTNNLNKHTSHLKDHSYASNPSQRSSSEDSASTEDEETATPSTPKLQVQLQTQMKQIKKFRDALRKTRKRYRAIVKAKTKQNAKFSRIFAKDQLESLSRVNMKGLKWSSYTVKKALQLRFACGSSGYKLLLQQHYPLPSERTLQRRMESVLFERGILREVFDYMQLKVKEMKEEERFCCITLDEMSLTSKYEFDPSSGDILGNVTGPRHSGNADHALVFMLGGITSRWKQTVAYHFTSSSTDGSVFHNIVLDIIQRASNIGLHVVAVTNDMGSANRAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CTM1</accession>
    <name>A0A9Q1CTM1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_04067</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CTM1"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="566"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="87"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="488"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="89"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8050753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1011" mass="115967" checksum="A13EB76B6A10AEB4" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CTR8</accession>
    <name>A0A9Q1CTR8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">FK506-binding protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_04200</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CTR8"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.340">
        <property type="entry name" value="Nucleoplasmin core domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041232">
        <property type="entry name" value="NPL"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17800">
        <property type="entry name" value="NPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleoplasmin-like" evidence="2">
        <location>
            <begin position="2"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="145"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="292"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="176"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="201"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="226"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="292"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8050843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="459" mass="51373" checksum="383F14F0EAA3C246" modified="2023-09-13" version="1">MIWGMTLHPGQELTRKVFGEEHLTLASLECRISKYQGKSRPSACLLLKTNMREHVICSLEKGVANQQCLDLIFEEGEKITFAAEGTGIIYLTGYTTKQEEGQNEMLEDQSDGIFLREKEDEEKDNDNNDKMPDNQEDGEILIKLEHDSEDGMQDSDQGGEEKDKEPPTSGNAGSGHDGDMSDHDHCGPVKDDDVDGGIGSSRCNDQGSTSDHGGDRGSDDNTGYHGDNKDNSGDDRDFHGDSIHDNSIQGDGNSCDHVDYREVHLSVAGVPGDGESMYDRDDDKIMDNLDKENQDAHDDEGKVGVSKDRKGSVNHQDGEEDEIIDRLCQEQQEWSVNLASQGIKGGVPRIPGASFEKENWDSASKFDNLKRFNKPVRKSKTGCKKRCIDFNYDRCKCCRKTLSEKRLHQTLRVKCIADPKRELHRKKHSSVSLRCSHFGKAVDYKCRNDNHKMFPLGVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CU11</accession>
    <name>A0A9Q1CU11_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SCAN box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_03976</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CU11"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF13">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCAN box" evidence="2">
        <location>
            <begin position="229"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="78"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8050679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="319" mass="38148" checksum="4ECC1A358D40C027" modified="2023-09-13" version="1">MPFNLDKFVASPSVEELDSLKKSEIVKVAKHYGIEFQPLMRKDEIKRYVLEYLVDESILPSTVLETAITVPTDNTFELKRLEIEINKEIRLKEIEREMQIMQMQWKREKEEREMQREKEAREHEFRMKQLELGVIKDSDPKVGLDTGVFNISKHVKFVPKFQEDNVEKFFNHFEKLGEQLKWPRDKWSILIQSNFTGKAQEVYSALSIEDSMDYDKVKKAILQAYELVPEAYRQKFRKYRKADTQTYVEFAYQKERHFDRWCASKKVSTFHTLRQLMLVEDFKRCVNDDIKTHLEENKADKLKEVANLADQYALTHKFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1GVC5</accession>
    <name>A0A9Q1GVC5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DoxX family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_32423</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DoxX family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1GVC5"/>
    <dbReference type="OrthoDB" id="432685at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040399">
        <property type="entry name" value="TMEM35A/B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13163">
        <property type="entry name" value="SPINAL CORD EXPRESSION PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13163:SF2">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 35B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040369195" description="DoxX family protein" evidence="7">
        <location>
            <begin position="20"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="57"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="82"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8027312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="139" mass="15244" checksum="D0F4C7237402A157" modified="2023-09-13" version="1">MGTMHYLAVGVGLCFAVLGLLKVAPIQPAHNQLVSNYGRFAGVFPLKFVGIQPSADMYTALMAFAEIMLGSLLAFGRYDWRAWSCLSLLVIMVIIVWSMLALGEVIAQMFPYLVLGGLLLVLLFHPNGFWGNRGKVHLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1GX30</accession>
    <name>A0A9Q1GX30_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein FAM76A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_32392</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM76 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1GX30"/>
    <dbReference type="OrthoDB" id="3689at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032017">
        <property type="entry name" value="FAM76"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46176">
        <property type="entry name" value="LD21662P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46176:SF1">
        <property type="entry name" value="LD21662P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16046">
        <property type="entry name" value="FAM76"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="126"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="228"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="132"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="151"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="189"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="228"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="246"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="259"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8027284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="268" mass="30973" checksum="7AF14E5B4343D42D" modified="2023-09-13" version="1">MFPQECRGSYPVVKCTYCRSEFQQVNKKSTDTICKKCAQNIKNYGMPKPCEYCSVIAAFIGTKCQKCSNSEKKYGPPHTCEQCKQKCAFDRKNDRKKVDGKLLCWLCTLSYKRVMQKAQKRKFDFISRDRNNSSPSNSPHSQHNTPSQNSQEKEKTKDKEKERWRPQQRDDKDHSREKTDDHRSKSRNKTGTLSTSSRLIDTGLPDQVVEVTRLREQVAQLKKSLDEKDRQILDKEKKARNRELQKQVSQLSRTQRPSAVAAAAANTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H2F7</accession>
    <name>A0A9Q1H2F7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Sorting nexin-27</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_29141</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H2F7"/>
    <dbReference type="OrthoDB" id="10036828at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000159">
        <property type="entry name" value="RA_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12431">
        <property type="entry name" value="SORTING NEXIN 17 AND 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12431:SF19">
        <property type="entry name" value="SORTING NEXIN-27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00788">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50200">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0967">Endosome</keyword>
    <keyword evidence="4" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="7">
        <location>
            <begin position="19"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-associating" evidence="8">
        <location>
            <begin position="126"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="281"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="288"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8029686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="315" mass="36317" checksum="3153ED745CB1BCFD" modified="2023-09-13" version="1">MAEEDTGKKRPTFSNEPRIVTIHKSESGFGFNVRGQVSEGGQLKSINGELYAPLQHVSAVLEGGAADRAGILRGDRILEVFAQSSDYHTYLSTVTVIAASETVREFLKTDLRDDREEEQPEEDNNRQVEMRIAVPGKDCTTVSISRSNSTEEVYKILVKQLKMDKDITDYFALFYLTKTKFARKLLTSEFPYNLYVQNQSNSQSKHTCIALRKWVFSPRKELFLNHDEMAMSFLYWQAQVPSFDWEEIKSFDIDDEGMALNFEYEKPGKKRRGVKVCTKYENDKAEADQESEEEEVEEEEEDEEEEEEDDEEGDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H2V1</accession>
    <name>A0A9Q1H2V1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RNase H type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_26478</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H2V1"/>
    <dbReference type="OrthoDB" id="7756796at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09275">
        <property type="entry name" value="RNase_HI_RT_DIRS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052055">
        <property type="entry name" value="Hepadnavirus_pol/RT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010998">
        <property type="entry name" value="Integrase_recombinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002156">
        <property type="entry name" value="RNaseH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041373">
        <property type="entry name" value="RT_RNaseH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33050">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33050:SF7">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17917">
        <property type="entry name" value="RT_RNaseH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47823">
        <property type="entry name" value="lambda integrase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50879">
        <property type="entry name" value="RNASE_H_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RNase H type-1" evidence="8">
        <location>
            <begin position="101"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8030151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="574" mass="65429" checksum="1E49D300450D5805" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H3E1</accession>
    <name>A0A9Q1H3E1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Beta-arrestin-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_27268</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the arrestin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H3E1"/>
    <dbReference type="OrthoDB" id="298939at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001664">
        <property type="term" value="F:G protein-coupled receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002031">
        <property type="term" value="P:G protein-coupled receptor internalization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.640:FF:000036">
        <property type="entry name" value="beta-arrestin-2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.840:FF:000003">
        <property type="entry name" value="Kurtz arrestin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.840">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000698">
        <property type="entry name" value="Arrestin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011021">
        <property type="entry name" value="Arrestin-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011022">
        <property type="entry name" value="Arrestin_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017864">
        <property type="entry name" value="Arrestin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014753">
        <property type="entry name" value="Arrestin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11792">
        <property type="entry name" value="ARRESTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11792:SF17">
        <property type="entry name" value="KURTZ ARRESTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02752">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00339">
        <property type="entry name" value="Arrestin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00309">
        <property type="entry name" value="ARRESTIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01017">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00295">
        <property type="entry name" value="ARRESTINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arrestin C-terminal-like" evidence="3">
        <location>
            <begin position="224"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="388"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8030766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="450" mass="50009" checksum="88BE3270BDAD0398" modified="2023-09-13" version="1">MSSEDKGDNHKSDSEDKEAPASNGVSGDSKKSGTRVFKKSSPNGKITTYLGKRDFVDHLEYIDPIDGVVLVDPEYLKDRKVYAHVLAAFRYGREDLDVLGLTFRKDLLLQTMQVYPPDPDAEKPLTKLQERLIKKLGKNAYPFFFQLPKNAPNSVTLQPAPGDTGKPCGVDYELKTYVAESVSEKPHKRNSVRLAIRKVTYAAEVPEEKRIQPQADKDKEFMLSSAPLHLEATLDKQQYYHGESIAVNVQITNNSNKTVKKIKISVRQLADICLFSSAQYKCPVAVLETEDGMPVGPSQTLSKVYTVTPLLSNNKDKRGLALDGKLKHEDTNLASSTLLTDVDENGKIADERRENLGIIVEYRVKVELKIAYSTANISVELPFKLSHPKPEEIETSPPQPADGETAPLDTNLISFETNEQNLVNADDDDLIFEDFARLRLQGAEGDGTDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H3P1</accession>
    <name>A0A9Q1H3P1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Netrin receptor UNC5B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_20911</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H3P1"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005042">
        <property type="term" value="F:netrin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.220.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037936">
        <property type="entry name" value="UNC5A-D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000906">
        <property type="entry name" value="ZU5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12582">
        <property type="entry name" value="NETRIN RECEPTOR UNC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12582:SF41">
        <property type="entry name" value="UNC5C-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00791">
        <property type="entry name" value="ZU5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51145">
        <property type="entry name" value="ZU5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0675">Receptor</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ZU5" evidence="1">
        <location>
            <begin position="9"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8034182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="369" mass="42624" checksum="C529ADD1AC88B5CB" modified="2023-09-13" version="1">MGRDWKSLLETKAVITKNGGVLEIPNTGVMLIIPPNALPEHMKHCEIHMRIIPWETVKEKATSFASNSSTAVELLPDRLKFQHPVRLTLPHCLVLQDTRERKAKIFISHHENESLPEWEEQTHLSYHLEDTACIILLDSLCWVKFSVDDEIVKAKKLIVYTAGRKLQPQDSVAEIRVGYYLDLPDEKMVESDARDLSVGHRKTFFFVRRKEKFPLEVSLDKIFPDDWQYHSKKPLLRKVPYEQISSFSRHSLIYTLKKESSMDGNLLCVFTVSQEKGQISVQLAAHLKYTRHQLFALRAGADKRIDASVYNLLKENGLLAYRGRRGGTHTYRRRPRREDFAVGNIPQLRFQTNPCGVILYTRVQSAYAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H4K4</accession>
    <name>A0A9Q1H4K4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ShKT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_25746</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H4K4"/>
    <dbReference type="OrthoDB" id="6132182at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003582">
        <property type="entry name" value="ShKT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01549">
        <property type="entry name" value="ShK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00254">
        <property type="entry name" value="ShKT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51670">
        <property type="entry name" value="SHKT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040149626" description="ShKT domain-containing protein" evidence="2">
        <location>
            <begin position="22"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="ShKT" evidence="3">
        <location>
            <begin position="341"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="341"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8032265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="377" mass="42337" checksum="6EC9DDD6012516AA" modified="2023-09-13" version="1">MYSSTVSILSLLLAFSVCVIASPVSRDTNQAGLNTDSKTFDYGDLEARFFSILDELDTQNPLEVRADRPGQASDQHRCDVISGVPRDVSSALRLNSFYRKYTHAYNIPVLGSFRVSDRALRRACYVVRFLLADRPDLRQAMYAKYGRVAIMATTEVTQNIPEHSFLPAFWNTRARGLGGTLQIPVSTGAEENVLCLRSDRYREDIFLHELAHGVHKIALTTAVPDYNRRLTNAYNSARRQGLWRNTYADDTVDEYFAEGVQSFFNVESPFVFGIHNDIDTREELASYDPTLYGLLREAFPCMNNIVDRCQNQDLIASQPLQMNCGGTTTGGTGGTGTNNNCVDNNQYCRDWANRGECQRNPGYMLINCALSCNQCRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H4L2</accession>
    <name>A0A9Q1H4L2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_26275</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H4L2"/>
    <dbReference type="OrthoDB" id="8939957at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8032683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="220" mass="24879" checksum="59C6EA05BD73C185" modified="2023-09-13" version="1">MDCPQAVYVELGKDGIINCRILNNGDVYWYKGNSTKTSPIIRLENGQKEIPQESSPYDIDKEGSLVIKNMEEKYYGLYSVVNFYANDKLDTGQIRVQIAVKPRTLCPVIAGCKDCNHCDLTEPETGNISCSIGGVRPQIHLNIVTDSENRINVIRNVSKQTYNTVTGTWNTTTFVEYYEWYDCTSPLHLRCVAGNEHPFELTDSVARIISGTLFNYFVLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H4M8</accession>
    <name>A0A9Q1H4M8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Kielin/chordin-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_25608</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6132182at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030513">
        <property type="term" value="P:positive regulation of BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00109">
        <property type="entry name" value="Kunitz-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.200.20">
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1280.10">
        <property type="entry name" value="Di-copper center containing domain from catechol oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008922">
        <property type="entry name" value="Di-copper_centre_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052424">
        <property type="entry name" value="Kielin_Chordin-BMP_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002223">
        <property type="entry name" value="Kunitz_BPTI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036880">
        <property type="entry name" value="Kunitz_BPTI_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020901">
        <property type="entry name" value="Prtase_inh_Kunz-CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048287">
        <property type="entry name" value="TSPN-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002227">
        <property type="entry name" value="Tyrosinase_Cu-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001007">
        <property type="entry name" value="VWF_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46698">
        <property type="entry name" value="CROSSVEINLESS 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46698:SF6">
        <property type="entry name" value="KIELIN_CHORDIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00264">
        <property type="entry name" value="Tyrosinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00093">
        <property type="entry name" value="VWC"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23334">
        <property type="entry name" value="VWC2L_2nd"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00131">
        <property type="entry name" value="KU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00210">
        <property type="entry name" value="TSPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00214">
        <property type="entry name" value="VWC"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="SMART" id="SM00215">
        <property type="entry name" value="VWC_out"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57362">
        <property type="entry name" value="BPTI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48056">
        <property type="entry name" value="Di-copper centre-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57603">
        <property type="entry name" value="FnI-like domain"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00280">
        <property type="entry name" value="BPTI_KUNITZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50279">
        <property type="entry name" value="BPTI_KUNITZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00497">
        <property type="entry name" value="TYROSINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01208">
        <property type="entry name" value="VWFC_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50184">
        <property type="entry name" value="VWFC_2"/>
        <property type="match status" value="14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040149256" evidence="9">
        <location>
            <begin position="25"/>
            <end position="2001"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="10">
        <location>
            <begin position="406"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="12">
        <location>
            <begin position="449"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="domain" description="VWFC" evidence="11">
        <location>
            <begin position="1158"/>
            <end position="1215"/>
        </location>
    </feature>
    <feature type="domain" description="VWFC" evidence="11">
        <location>
            <begin position="1215"/>
            <end position="1274"/>
        </location>
    </feature>
    <feature type="domain" description="VWFC" evidence="11">
        <location>
            <begin position="1274"/>
            <end position="1331"/>
        </location>
    </feature>
    <feature type="domain" description="VWFC" evidence="11">
        <location>
            <begin position="1332"/>
            <end position="1391"/>
        </location>
    </feature>
    <feature type="domain" description="VWFC" evidence="11">
        <location>
            <begin position="1392"/>
            <end position="1450"/>
        </location>
    </feature>
    <feature type="domain" description="VWFC" evidence="11">
        <location>
            <begin position="1450"/>
            <end position="1508"/>
        </location>
    </feature>
    <feature type="domain" description="VWFC" evidence="11">
        <location>
            <begin position="1508"/>
            <end position="1568"/>
        </location>
    </feature>
    <feature type="domain" description="VWFC" evidence="11">
        <location>
            <begin position="1568"/>
            <end position="1626"/>
        </location>
    </feature>
    <feature type="domain" description="VWFC" evidence="11">
        <location>
            <begin position="1628"/>
            <end position="1688"/>
        </location>
    </feature>
    <feature type="domain" description="VWFC" evidence="11">
        <location>
            <begin position="1688"/>
            <end position="1747"/>
        </location>
    </feature>
    <feature type="domain" description="VWFC" evidence="11">
        <location>
            <begin position="1747"/>
            <end position="1805"/>
        </location>
    </feature>
    <feature type="domain" description="VWFC" evidence="11">
        <location>
            <begin position="1807"/>
            <end position="1867"/>
        </location>
    </feature>
    <feature type="domain" description="VWFC" evidence="11">
        <location>
            <begin position="1867"/>
            <end position="1925"/>
        </location>
    </feature>
    <feature type="domain" description="VWFC" evidence="11">
        <location>
            <begin position="1925"/>
            <end position="1983"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="275"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="326"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="275"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="414"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="433"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8032161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="2001" mass="223373" checksum="95E390D3BDCC47DA" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H4V1</accession>
    <name>A0A9Q1H4V1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">THO complex subunit 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_23552</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H4V1"/>
    <dbReference type="OrthoDB" id="10257415at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000445">
        <property type="term" value="C:THO complex part of transcription export complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01670">
        <property type="entry name" value="Death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000488">
        <property type="entry name" value="Death_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021861">
        <property type="entry name" value="THO_THOC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13265:SF0">
        <property type="entry name" value="HPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13265">
        <property type="entry name" value="THO COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00531">
        <property type="entry name" value="Death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11957">
        <property type="entry name" value="efThoc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00005">
        <property type="entry name" value="DEATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50017">
        <property type="entry name" value="DEATH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Death" evidence="2">
        <location>
            <begin position="592"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="419"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="420"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8033344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="685" mass="78952" checksum="20BD7BB218243F13" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H4X2</accession>
    <name>A0A9Q1H4X2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Zinc finger MYM-type protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_25991</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H4X2"/>
    <dbReference type="OrthoDB" id="6621481at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025398">
        <property type="entry name" value="DUF4371"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45749">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45749:SF21">
        <property type="entry name" value="DUF4371 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14291">
        <property type="entry name" value="DUF4371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4371" evidence="1">
        <location>
            <begin position="2"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8032461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="367" mass="41675" checksum="769CA6A1B9CA03E5" modified="2023-09-13" version="1">MDEATDVTHHEQAAIIVRYVDKDMTIQERLIWVTAVENTTAENLLQTLLGALKMVEIDTSKIVSQCYDGAANMSGGISGVQSRLKEIQPRAIYVHCFGHCLNLVIADAMAEVQSTRNFFGSVQSIYNFIESSPHRHAILESVMKRLLTDKRIKTLKSLSETRWTCRAEAVNSIAENLQSILQALEEITERTTRPRVMAEAQAILKNISTFEFVLHMVALQPLLNKCKIVSNFLQSEDLDLVTALETVASLKENLLQMRSDEEASAIFSRAKTLCDGLCIDADIPRRPRKVSRRIDDCSENEHEFTSAQEEWKVRSYFQVIDKAVADIDRRFNQEATDIIRTCGKILRKEKPMSSTRSPHNLMYHQLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H6U4</accession>
    <name>A0A9Q1H6U4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_21472</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H6U4"/>
    <dbReference type="OrthoDB" id="10527351at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8034575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="134" mass="15108" checksum="CEBFE3D2C3424EDA" modified="2023-09-13" version="1">MDSLIPDIPLSPVLQDSSDEESRIQPGQKSPSRKKSERIRLENDTFKKIKSHSAPFTKIQATLISLTSEIKRLDHQLEKGNIPKHLQVQRNIPELPGSLACSEELRDIWGSHLQRAGKRLGKLDVRSSPARQKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H7K2</accession>
    <name>A0A9Q1H7K2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Adhesion G protein-coupled receptor B3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_22512</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H7K2"/>
    <dbReference type="OrthoDB" id="10045417at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.220.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1240.10">
        <property type="entry name" value="GPCR, family 2, extracellular hormone receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051587">
        <property type="entry name" value="Adhesion_GPCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046338">
        <property type="entry name" value="GAIN_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036445">
        <property type="entry name" value="GPCR_2_extracell_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45813">
        <property type="entry name" value="IG-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45813:SF8">
        <property type="entry name" value="IG-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="10"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="386"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="419"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="453"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="486"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="526"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
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        <source>
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        </source>
    </evidence>
    <sequence length="795" mass="88035" checksum="73112844A2672883" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
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            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
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    </reference>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H8Y8</accession>
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                <person name="Chen T."/>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H8Y8"/>
    <dbReference type="OrthoDB" id="10043687at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15517">
        <property type="entry name" value="PHD_TCF19_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050863">
        <property type="entry name" value="CenT-Element_Derived"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004875">
        <property type="entry name" value="DDE_SF_endonuclease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19303:SF74">
        <property type="entry name" value="POGO TRANSPOSABLE ELEMENT WITH KRAB DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19303">
        <property type="entry name" value="TRANSPOSON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03184">
        <property type="entry name" value="DDE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE-1" evidence="2">
        <location>
            <begin position="135"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="394"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="409"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8037020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="489" mass="55498" checksum="4DFBD8A568D2715D" modified="2023-09-13" version="1">MLKDHIIHLAKLGYPLTRSDVLQIATQTAVFLKVKEDPSHLLSQHWLNGFLSRWLELKVVRPSKLGMKCAKAITKESISKYFNELNNILIKYELKDKPHLIFNVDETGFNFEHRPTKVVAPRGIKVNAITTDKGAITTILAAGNAVGYAIPPYFIFKGKHMIERLKAGAYPGSGFEMTSSGWSNGEVFINYIENHFTKYLPVRDPNQHILLLYDGHTSHISLNLINNAQSKKIILFVLPPHSSHLLQPLDVATFKPLKDFYHHECQTYMRKNPGQVITRYEVCQLISKAYLKAFTPSNLIASFRKSGIFPYKKDVIDEHHFLGDALRPKVPGPPPDTPAANSDYRKFLDHLLPQPKPIETKKRKVGYRPGGVAITEEKIILKIKKVIQEKESVNKKKINPQQPQKEPSYEELDTSSSDESEMSEGDVCCICKRFSPPTLRGVLRRQVTFVDWGQCSCGHWTHLQFCVPENVDEIMNAGDFSCPHCKGEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HBU6</accession>
    <name>A0A9Q1HBU6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein NLRC5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_17294</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HBU6"/>
    <dbReference type="OrthoDB" id="427518at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="CDD" id="cd01120">
        <property type="entry name" value="RecA-like_superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312">
        <property type="entry name" value="NACHT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312:SF2">
        <property type="entry name" value="NUCLEOTIDE-BINDING OLIGOMERIZATION DOMAIN-CONTAINING PROTEIN 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NACHT" evidence="1">
        <location>
            <begin position="58"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8039541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="574" mass="65367" checksum="D47AF35CCF7E8FD9" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HCG7</accession>
    <name>A0A9Q1HCG7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_14593</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HCG7"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8040333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="186" mass="20153" checksum="56807C8A9199AC3C" modified="2023-09-13" version="1">MCYGKRKNPIIFGGGGRSSGVTGGQNLKILCARYLKMCYGKRKYPIVFGGGQRSSGVTGGQNLKILCARYLKMCYGKRKKPIVFDGGRRSSGVTGGQNLKILCARYLKMCYGKRKKPIVFGGGRRSSGVTGGQNLKILCARYLKMCYGKRKNPIVFGGGQKSSGVTGGQNLKILCARYLKVGRKLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HCV1</accession>
    <name>A0A9Q1HCV1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="6">5.6.2.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">DNA 3'-5' helicase BLM</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_12257</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. RecQ subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="6"/>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HCV1"/>
    <dbReference type="OrthoDB" id="10066805at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710">
        <property type="entry name" value="DNA HELICASE RECQ FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710:SF153">
        <property type="entry name" value="RECQ-LIKE DNA HELICASE BLM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0347">Helicase</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase C-terminal" evidence="8">
        <location>
            <begin position="143"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8041444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="362" mass="40377" checksum="7520402390642F27" modified="2023-09-13" version="1">MVSDSNDVMDRVCEGRYSLLFASPEVLLSSKGKELMMGTHVYHRLCCLFVDESHCIVKWGQSSGNTEAFRKYYGMIAELRSVIKLCTPVVAMTATATQLVRSKIVQALGMNSFVLIDSNLIVQNIKHIVITPSQNDYPKIFDWLCDKIVREKEQTERVLIFCQSRKDCSELYSLFCSKLPQALHHHFNMYHTNTENDIQEGIVKDFADVNGKVRVLVCTIAFGLGVNVKGVHTVIMYGTPCEIDDYVQLCGRGGRDGLQSISVTLKMSQTGRAVSKEMKDFTKTSGCRHEILYKAFSEDSCPSVLGHNCCDNCSLHCSCAGTGCSAKPTLLEQKIASHQSECEIPIIQVSDDPYRNIMLPAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HDI1</accession>
    <name>A0A9Q1HDI1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_08477</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HDI1"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8045462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="61" mass="6928" checksum="304C58F57838F051" modified="2023-09-13" version="1">MNKSIRPQRRGNCVEGELTSITAEAYMKRTVSPAAWAIMSEMSKRILIYGVGLIWTLFITH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HE89</accession>
    <name>A0A9Q1HE89_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Bifunctional polynucleotide phosphatase/kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_12880</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HE89"/>
    <dbReference type="OrthoDB" id="19045at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046404">
        <property type="term" value="F:ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046403">
        <property type="term" value="F:polynucleotide 3'-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01625">
        <property type="entry name" value="HAD_PNP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000078">
        <property type="entry name" value="Bifunctional polynucleotide phosphatase/kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000737">
        <property type="entry name" value="Bifunctional polynucleotide phosphatase/kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041388">
        <property type="entry name" value="FHA_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006549">
        <property type="entry name" value="HAD-SF_hydro_IIIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013954">
        <property type="entry name" value="PNK3P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006550">
        <property type="entry name" value="PNKP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006551">
        <property type="entry name" value="Polynucleotide_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01664">
        <property type="entry name" value="DNA-3'-Pase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01662">
        <property type="entry name" value="HAD-SF-IIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01663">
        <property type="entry name" value="PNK-3'Pase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12083">
        <property type="entry name" value="BIFUNCTIONAL POLYNUCLEOTIDE PHOSPHATASE/KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12083:SF9">
        <property type="entry name" value="BIFUNCTIONAL POLYNUCLEOTIDE PHOSPHATASE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13671">
        <property type="entry name" value="AAA_33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17913">
        <property type="entry name" value="FHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08645">
        <property type="entry name" value="PNK3P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FHA" evidence="7">
        <location>
            <begin position="34"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="124"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="186"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="128"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="151"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="186"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8041941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="612" mass="68917" checksum="5E069870F2FFA264" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HE91</accession>
    <name>A0A9Q1HE91_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_05010</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HE91"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8046362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="96" mass="11036" checksum="DCE6126444D30CF4" modified="2023-09-13" version="1">MMECYHENHLSRTRNLFTGYECQPILNECQPIHLQHRDRQPAKAEVQMLAGHTVLDIQKKRSTYLHISVVAGGSPSMKLYDCIKRQSSEVLPLDIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HEB5</accession>
    <name>A0A9Q1HEB5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_12979</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8042016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HEB5"/>
    <dbReference type="OrthoDB" id="10066771at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24379:SF121">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24379">
        <property type="entry name" value="KRAB AND ZINC FINGER DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="376"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="778"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="902"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="930"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="1080"/>
            <end position="1105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="61"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="134"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="415"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="514"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="11"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="86"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="134"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="161"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8042016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1105" mass="122346" checksum="1BD085A626401FB2" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HEL5</accession>
    <name>A0A9Q1HEL5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription elongation factor SPT6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Transcription elongation factor spt6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_10103</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Histone H3-H4 chaperone that plays a role in maintenance of chromatin structure during RNA polymerase II transcription elongation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the SPT6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="343921at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008023">
        <property type="term" value="C:transcription elongation factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034728">
        <property type="term" value="P:nucleosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140673">
        <property type="term" value="P:transcription elongation-coupled chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00164">
        <property type="entry name" value="S1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09928">
        <property type="entry name" value="SH2_Cterm_SPT6_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09918">
        <property type="entry name" value="SH2_Nterm_SPT6_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000494">
        <property type="entry name" value="Suppressor of Ty 6 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2740:FF:000001">
        <property type="entry name" value="Transcription elongation factor spt6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.650:FF:000002">
        <property type="entry name" value="Transcription elongation factor spt6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.850:FF:000001">
        <property type="entry name" value="Transcription elongation factor spt6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3500.10:FF:000006">
        <property type="entry name" value="Transcription elongation factor spt6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.140:FF:000004">
        <property type="entry name" value="Transcription elongation factor spt6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000030">
        <property type="entry name" value="Transcription elongation factor spt6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000089">
        <property type="entry name" value="Transcription elongation factor spt6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.650">
        <property type="entry name" value="RuvA domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2740">
        <property type="entry name" value="Spt6, Death-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.850">
        <property type="entry name" value="Spt6, helix-hairpin-helix domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3500.10">
        <property type="entry name" value="Tex N-terminal region-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.140">
        <property type="entry name" value="YqgF/RNase H-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041692">
        <property type="entry name" value="HHH_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028083">
        <property type="entry name" value="Spt6_acidic_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042066">
        <property type="entry name" value="Spt6_death-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032706">
        <property type="entry name" value="Spt6_HHH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028088">
        <property type="entry name" value="Spt6_HTH_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035420">
        <property type="entry name" value="Spt6_SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035018">
        <property type="entry name" value="Spt6_SH2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035019">
        <property type="entry name" value="Spt6_SH2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028231">
        <property type="entry name" value="Spt6_YqgF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055179">
        <property type="entry name" value="Tex-like_central_region"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023323">
        <property type="entry name" value="Tex-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023319">
        <property type="entry name" value="Tex-like_HTH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017072">
        <property type="entry name" value="TF_Spt6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037027">
        <property type="entry name" value="YqgF/RNaseH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10145">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR SPT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10145:SF6">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR SPT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14635">
        <property type="entry name" value="HHH_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17674">
        <property type="entry name" value="HHH_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14641">
        <property type="entry name" value="HTH_44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14633">
        <property type="entry name" value="SH2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14632">
        <property type="entry name" value="SPT6_acidic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22706">
        <property type="entry name" value="Tex_central_region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14639">
        <property type="entry name" value="YqgF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036947">
        <property type="entry name" value="Spt6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="RuvA domain 2-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158832">
        <property type="entry name" value="Tex N-terminal region-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="12" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="S1 motif" evidence="11">
        <location>
            <begin position="1255"/>
            <end position="1310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="212"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="486"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1315"/>
            <end position="1346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1636"/>
            <end position="1790"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="7"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="69"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="99"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="128"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="154"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="179"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="217"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="240"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="494"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1327"/>
            <end position="1346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1636"/>
            <end position="1707"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1709"/>
            <end position="1729"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00070625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR036947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8043140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1790" mass="205927" checksum="AC7462357C2A5697" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HFI4</accession>
    <name>A0A9Q1HFI4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">G/T mismatch-specific thymine DNA glycosylase</fullName>
            <ecNumber evidence="15">3.2.2.29</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Thymine-DNA glycosylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">HOLleu_06981</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">Nanhai2018</strain>
            <tissue evidence="20">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>Hydrolyzes mismatched double-stranded DNA and polynucleotides, releasing free thymine.</text>
            <dbReference type="EC" id="3.2.2.29"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="14">Homodimer. Interacts with AICDA and GADD45A.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the uracil-DNA glycosylase (UDG) superfamily. TDG/mug family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.29" evidence="15"/>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HFI4"/>
    <dbReference type="OrthoDB" id="565731at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141016">
        <property type="term" value="F:G/T mismatch-specific thymine-DNA glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032183">
        <property type="term" value="F:SUMO binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004844">
        <property type="term" value="F:uracil DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006285">
        <property type="term" value="P:base-excision repair, AP site formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040029">
        <property type="term" value="P:epigenetic regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd10028">
        <property type="entry name" value="UDG-F2_TDG_MUG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.470.10:FF:000002">
        <property type="entry name" value="G/T mismatch-specific thymine DNA glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.470.10">
        <property type="entry name" value="Uracil-DNA glycosylase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015637">
        <property type="entry name" value="MUG/TDG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005122">
        <property type="entry name" value="Uracil-DNA_glycosylase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036895">
        <property type="entry name" value="Uracil-DNA_glycosylase-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12159">
        <property type="entry name" value="G/T AND G/U MISMATCH-SPECIFIC DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12159:SF9">
        <property type="entry name" value="G_T MISMATCH-SPECIFIC THYMINE DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03167">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52141">
        <property type="entry name" value="Uracil-DNA glycosylase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="6" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Uracil-DNA glycosylase-like" evidence="19">
        <location>
            <begin position="101"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="40"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="274"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="439"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="18">
        <location>
            <begin position="45"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="284"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="306"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="322"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="450"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00052915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00061261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00064519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00066769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00071248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00083221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF03167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAJ8047862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="465" mass="52193" checksum="B9DE59AAAFDC1B70" modified="2023-09-13" version="1">MQGNMDMYQQYQYNGYPPLPPVKQEPMDYPPPYPMEYMDMNNYQPLPPVPPPLEEPQPKVKKRKTKQDKVTDHFKEKKPRKKIDRFKGMPEEEVAKRTLPDHLKEGLDIVIIGINPGLMAAFKGHHYAGPGNHFWKCLYLSGMTSEPMTAMDDFKLLDIGIGFTNMVERTTRGSADLTRKEIKEGAQIMIEKIRKYKPLIACFNGKGIYEIFSGKKDFEVGRQPETIPGTDTVIYVMPSSSARCSQFPRAQDKVQFYVTLRNLRDELKGLKTNNGIPKALPCGSPNQSEASETNSTQTSVPAKNDQPVKIKEEKTDGDESYKTCQSTKQDVPCSSQQSVPVKSEPLESKDYITLQPMNPLDSSFIHNSMSSYADNFTVKQEPLDNPFTCPGNGFLPTIKQEISPSSFSGPNSQQEWPTMDLGNFIDDIPPIASNAAVPVTEEEYSVQEGRASQPQFSDGNISASY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HH00</accession>
    <name>A0A9Q1HH00_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">mRNA decay activator protein ZFP36L1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_08480</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HH00"/>
    <dbReference type="OrthoDB" id="410307at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000001">
        <property type="entry name" value="zinc finger CCCH domain-containing protein 15-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000002">
        <property type="entry name" value="Zinc finger protein 36, C3H1 type-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045877">
        <property type="entry name" value="ZFP36-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547">
        <property type="entry name" value="CCCH ZINC FINGER/TIS11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547:SF18">
        <property type="entry name" value="PROTEIN TIS11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="118"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="156"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="118"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="156"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="87"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="182"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="269"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="307"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="92"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="315"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="337"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8045465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="366" mass="40780" checksum="B3DFA3D13D965981" modified="2023-09-13" version="1">MSSTLVSPYFDSANDVFSRFNDRTPDAFSPTNSFSMNYDKQLFGSSIGFDEMKRQSSGSSFPLMRSMSCSVSTKSILPIGTDSVFNSNNFDNSKETRPRDRAFSESDRNQRNNQNSNRYKTELCRPFEENGKCKYGDKCQFAHGIHEIRNLQRHPKYKTELCRTYHTVGICPYGPRCHFIHSPSERKNYSNTSPKQRRDRTVERPKLLPFSSVPLGSTGDLTPPPSVCDSPSPFSPYGGHDGMEDNFLEALTAQARELATGDHTELLMSPRGMSPTLPSPIQSAPGSPFPPGHGFGVDMQLKDNVFFSSGVDSKPPSPIAYRPPSPPDSPDPETFCDRSSSPTGSAGNTRSSRLPIFRVMSMEEYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HJI5</accession>
    <name>A0A9Q1HJI5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_01991</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HJI5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8049304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="119" mass="13734" checksum="86DEDE8B08CE6DD0" modified="2023-09-13" version="1">MRGFDRIPVHDLFQLKSHQKSCLITRGHALSILKQRPHLMLRRYYFSQRVIDDWNSLPPEAVKAKTVLQFKKSIGPLFKKLGGHTTSLRKLSAPLTNRATTVGTLCLTRPSSWWTRYST</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0Y9H1</accession>
    <name>A0A9Q0Y9H1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_43571</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000364">
        <property type="protein sequence ID" value="KAJ8018443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0Y9H1"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="45"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8018443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="139" mass="16430" checksum="0EB1775B9CA2D355" modified="2023-09-13" version="1">MRRPHNCYLVVRKANAGNNGGRFNVANDVCGLKIGSVEGKHRGWFDEIEEDIEKLIDKLQKAHKDRLDDKTSSKKKQDYQQVRQILQQELRKMNNDWWKKKAIKGATSRSRQLRHEDISWWPSHRLLIQGIWLKPSPIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0Y9T2</accession>
    <name>A0A9Q0Y9T2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_45077</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010001780">
        <property type="protein sequence ID" value="KAJ8017486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0Y9T2"/>
    <dbReference type="OrthoDB" id="6502630at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8017486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="140" mass="16018" checksum="8E5A32B222BF76DB" modified="2023-09-13" version="1">MHYGKRKNPIVFGGGQRSFGVTRGQTLKTLFTRYLKMHYGKRKNPIVFGGGQRSFGVTRGQTLKTLFTRYLKMHYGKRKNRIVFGGGQRSFEVTRGQTLKTLFTRYLKMHYGKRKNAVIFGGGQRSSGVTRGQILKTLFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YAA1</accession>
    <name>A0A9Q0YAA1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_42701</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000120">
        <property type="protein sequence ID" value="KAJ8018983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YAA1"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8018983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="144" mass="16409" checksum="14B0B5B0E0F07179" modified="2023-09-13" version="1">MEALMVQVVLHCVYKFYECVNYYHMMEQLGYMMTTCTKAGFTFPEVISADIFTLQAWSVLKSGTLGEDLLPPKLVRGHVTAGKLNARLTMAQPVNLDGEGLSAEHGKHKESVTQNCKEDFLRKNVRFGRTIQLKRGLSHTRHYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YAE4</accession>
    <name>A0A9Q0YAE4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_43752</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000441">
        <property type="protein sequence ID" value="KAJ8018325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YAE4"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8018325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="56" mass="6645" checksum="766EAA0434A1DC7C" modified="2023-09-13" version="1">MIRSSVGWLVIRMMPHYCKKTWIGYFHGLRLGKCLFNADKCKVLHFGPKNFSYDYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YAR6</accession>
    <name>A0A9Q0YAR6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_42102</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000032">
        <property type="protein sequence ID" value="KAJ8019342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YAR6"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8019342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="88" mass="10367" checksum="0915F087DDF46659" modified="2023-09-13" version="1">MTKHVNNLVSSCNYYLRNLFRIGRYLDKETRHSVAVLFSFHALTTAMPFCMVRNQMTLIAFNPCKIVVLNLFSRQLDYPYKKSQIFPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YB91</accession>
    <name>A0A9Q0YB91_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein CASC3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">HOLleu_41193</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">Nanhai2018</strain>
            <tissue evidence="20">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Stress granule</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus speckle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the CASC3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8019553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YB91"/>
    <dbReference type="OrthoDB" id="657902at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035145">
        <property type="term" value="C:exon-exon junction complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018545">
        <property type="entry name" value="Btz_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028544">
        <property type="entry name" value="CASC3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13434">
        <property type="entry name" value="PROTEIN CASC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13434:SF0">
        <property type="entry name" value="PROTEIN CASC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09405">
        <property type="entry name" value="Btz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01044">
        <property type="entry name" value="Btz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="17" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="15" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="11" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="14" id="KW-0866">Nonsense-mediated mRNA decay</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="10" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="12" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Btz" evidence="19">
        <location>
            <begin position="172"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="93"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="789"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="35"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="67"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="107"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="142"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="164"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="182"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="215"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="235"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="18">
        <location>
            <begin position="286"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="315"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="339"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="390"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="413"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="428"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="460"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="513"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="552"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="570"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="642"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="687"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="713"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="18">
        <location>
            <begin position="800"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="817"/>
            <end position="835"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00019964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM01044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAJ8019553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="866" mass="98110" checksum="2926745B64F4984D" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YDK2</accession>
    <name>A0A9Q0YDK2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_44213</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000713">
        <property type="protein sequence ID" value="KAJ8018027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YDK2"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8018027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="76" mass="8774" checksum="91116930C5141DB1" modified="2023-09-13" version="1">MESSREFNFNAAFLELSRRENCLRGLTTLLHCLLPCIQVIRSWNQFLRYSTLQVYHCAIVNVHALPALGPDQANIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YEV3</accession>
    <name>A0A9Q0YEV3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_43068</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000200">
        <property type="protein sequence ID" value="KAJ8018762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YEV3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8018762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="301" mass="34830" checksum="75EB2709CD43EEDA" modified="2023-09-13" version="1">MTSSPVLLLTLCVLSMIATSAFWFFKGQCRGRKSDVGSKKSSEDEESISLTFPGENMTQEIFEKELAIELSMLKMEIAQNLGVEETLRLSSRLHFCPEEQNSINSSKNPSVTLMKMLCNGSANSVMHKLAILEVTTRKLEMTEINKVVDGFIRNHFRSTFMLKVDIARDGKPTYEHEAQRLWEKVLRDYSQCANIVDLQNFFENEYDATTSSILEGSLLIRLKISSAKCLKKLWRDIRSGQLRLRISPFFVNFNQLQKDRNMPFELYITYDEKAYKILQAKLQVFMFPFRGEFSRARNIHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YFX2</accession>
    <name>A0A9Q0YFX2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Protein phosphatase 1 regulatory subunit 35</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_38975</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
            <location evidence="1">Centriole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PPP1R35 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YFX2"/>
    <dbReference type="OrthoDB" id="8942190at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019902">
        <property type="term" value="F:phosphatase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903724">
        <property type="term" value="P:positive regulation of centriole elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045724">
        <property type="term" value="P:positive regulation of cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033590">
        <property type="entry name" value="PPP1R35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029135">
        <property type="entry name" value="PPP1R35_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28625">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28625:SF1">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15503">
        <property type="entry name" value="PPP1R35_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein phosphatase 1 regulatory subunit 35 C-terminal" evidence="6">
        <location>
            <begin position="208"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="142"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="198"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00029452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF15503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8021700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="355" mass="39472" checksum="46951EE4776D5AA1" modified="2023-09-13" version="1">MNDPGNIISDEEYLPLPKQLQPIPASKWTDFSPSGISDPALQLTPEKGVKLTHLATNKNDSFPAGRTETYKEYVDKVPTVSPLKTSKAECNNNLDGSKELQISAWVEKHHDNTTVYNTCTTHSEGSFPENVAVKGHKDKRVKKSGGCQDVFRKSNHVKSGMSSKQNIDGEKKLKPILHKTPRQSSGGNEKGTSKKHVRLAESSDLDKPQLHSTTALEAKLRQVEGETFDSRKAIEKNLGESEEARSLFAGRIAGGVNVPKNVNKYSGLASIDVSKQAVLDQAVEERLMKLKIRPEDTRRDSEPPPPDVLDVFTSDMIVEYPDCDISCVTLPSQTLQQVPMEEVFYLYERIQGWEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YH97</accession>
    <name>A0A9Q0YH97_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_37516</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YH97"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8022573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="111" mass="12144" checksum="59E7B88C81951599" modified="2023-09-13" version="1">MTVQFGPSKVYSSSMLHRLKKFPSACLIWLVGHGLPTPDLKGENSMLPARISCITCYQDSTIVQHVASMLLLSNMLPRLATVQHVASTQQLLNMLPGQCSFSSMPPGHGNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YHT0</accession>
    <name>A0A9Q0YHT0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Bone morphogenetic protein 2-B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_37769</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the TGF-beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YHT0"/>
    <dbReference type="OrthoDB" id="5987191at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13756">
        <property type="entry name" value="TGF_beta_BMPs_GDFs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.90.10:FF:000001">
        <property type="entry name" value="Bone morphogenetic protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001839">
        <property type="entry name" value="TGF-b_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001111">
        <property type="entry name" value="TGF-b_propeptide"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015615">
        <property type="entry name" value="TGF-beta-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017948">
        <property type="entry name" value="TGFb_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848:SF308">
        <property type="entry name" value="BMP-LIKE PROTEIN UNC-129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848">
        <property type="entry name" value="TGF-BETA FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00019">
        <property type="entry name" value="TGF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00688">
        <property type="entry name" value="TGFb_propeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00204">
        <property type="entry name" value="TGFB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00250">
        <property type="entry name" value="TGF_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51362">
        <property type="entry name" value="TGF_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0339">Growth factor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040205093" evidence="9">
        <location>
            <begin position="24"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="domain" description="TGF-beta family profile" evidence="10">
        <location>
            <begin position="316"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8022780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="431" mass="47636" checksum="DABD84577D81B507" modified="2023-09-13" version="1">MDSRPFLCALLSFFVCAPSNTSAYPTDFVQEFPELLDRNLNLPQDSSSQQMVQGATGSVFSEGAAISSESVTVETNTLEQISQVFGIANLTGIKAAGKGKRAGTPSFMLNLYNSVANSTNGEARAATPYLANTIRCVTNEKGRRKNRQVLQFKIDVVPPSERLLNAELYLFRLMSKLRDNSERRKSYQVSIYQRTNTEGTKLIDAKRVSSHGTGWETFNVGPSVREWFADPDTNLGFYINVTTLEGNAVPEGSIRFCLKNIERRPNLVLFTEDNTQESFSSVEGSFPNLPDVPVYPTSPIDNEPLPTHDVTPQHQLNKREVKPTDGDQKLCGLASMFVDFERLGWSDQIVAPNGFSANRCVGQCSFPAKMDIPYSTHALVQSFMAMETPEVGTACCVPHVLGSLTFLYFDDHSNIVLKRVRNAVAVKCGCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YIB2</accession>
    <name>A0A9Q0YIB2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_37623</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YIB2"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8022659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="89" mass="10235" checksum="240E2A5B88E3120E" modified="2023-09-13" version="1">MIYFDHVTMFLLVNCEIQGKFFLALRIICNSYVQNVFRGACNAMKGDLQEFANFSKENFGATAYCLLAVGAHLDKTSRLLRSSFRHFNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YIM0</accession>
    <name>A0A9Q0YIM0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_38400</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YIM0"/>
    <dbReference type="OrthoDB" id="6343797at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46585:SF1">
        <property type="entry name" value="CHROMO DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46585">
        <property type="entry name" value="INTEGRASE CORE DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8021254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="103" mass="11747" checksum="7D694D431BBE164A" modified="2023-09-13" version="1">MSTATLPVILKRIHYDASPPPGFRDVDALLRHARQKRPTTTRQEVKSWLETQDTYTLHKPNGVMVSGFDDQCHADLVDVRALSRYNRGIKFLLTCIGIFSKYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YJB5</accession>
    <name>A0A9Q0YJB5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mediator of RNA polymerase II transcription subunit 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Mediator complex subunit 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_41585</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Mediator complex subunit 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8019831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2281547at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051999">
        <property type="entry name" value="Mediator_complex_subunit_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019680">
        <property type="entry name" value="Mediator_Med1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12881">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12881:SF10">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10744">
        <property type="entry name" value="Med1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Mediator complex subunit Med1" evidence="10">
        <location>
            <begin position="77"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="547"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="663"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="729"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="789"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="977"/>
            <end position="1680"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1692"/>
            <end position="1731"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1744"/>
            <end position="2206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="2254"/>
            <end position="2424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="560"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="734"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="758"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="789"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="839"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="868"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1011"/>
            <end position="1027"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1043"/>
            <end position="1063"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="1081"/>
            <end position="1092"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="1131"/>
            <end position="1140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1213"/>
            <end position="1227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1230"/>
            <end position="1289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1290"/>
            <end position="1300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1325"/>
            <end position="1334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1370"/>
            <end position="1415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1428"/>
            <end position="1440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1441"/>
            <end position="1461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1480"/>
            <end position="1493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1494"/>
            <end position="1512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1521"/>
            <end position="1554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1555"/>
            <end position="1571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1580"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1603"/>
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    </feature>
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        <location>
            <begin position="1642"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1692"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1715"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1797"/>
            <end position="1818"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1819"/>
            <end position="1844"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1852"/>
            <end position="1886"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1887"/>
            <end position="1903"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1919"/>
            <end position="1943"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1975"/>
            <end position="1987"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1991"/>
            <end position="2000"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="2002"/>
            <end position="2017"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="2070"/>
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        <location>
            <begin position="2109"/>
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            <dbReference type="ARBA" id="ARBA00004123"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00006210"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
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        <source>
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        </source>
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        <source>
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        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00031254"/>
        </source>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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        <source>
            <dbReference type="Pfam" id="PF10744"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAJ8019831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="2442" mass="258437" checksum="C1D9930572AC4168" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YK69</accession>
    <name>A0A9Q0YK69_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nbr1 FW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_36532</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="661148at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
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        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14947">
        <property type="entry name" value="NBR1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14349">
        <property type="entry name" value="UBA_CF106"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Chromosome 6 C6orf106 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="C6orf106_UBA-like"/>
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        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
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        <property type="entry name" value="Nbr1_FW"/>
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    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20930">
        <property type="entry name" value="OVARIAN CARCINOMA ANTIGEN CA125-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN ILRUN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="UBA_4"/>
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    </dbReference>
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        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nbr1 FW" evidence="2">
        <location>
            <begin position="89"/>
            <end position="186"/>
        </location>
    </feature>
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        <location>
            <begin position="259"/>
            <end position="287"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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        <source>
            <dbReference type="Pfam" id="PF16158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8023947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="306" mass="34235" checksum="C4D514323C1CCF1E" modified="2023-09-13" version="1">MEVETEKDVEMELDGDFMQKFSCLGTTDKDVLISEFQRLAGTGVNPTACAFFLDMNNWNLQAAICSYYDFENPAIEMPMPTMTFIQDITIGEGESVPPDTDFTKTWRVQNSGTEAWPQGSYLRFLQGHILGSNERQEVRSLQPQEYANISIQMHSPKECGLYQGQWRMCAQNGMFFGETIWVILEVKEGGLLGLTQQMSSMGSEHFSHVSPNAAVSNPFASSTETDLSNSINSIQLGGASNIDPSQSPVRPTLFQLPQHRHQDTQPHPINSLQPHPDFPPSDVDRYHHTNSQEHLTNHHQSGGNIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YLF3</accession>
    <name>A0A9Q0YLF3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uronyl 2-sulfotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_34448</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sulfotransferase 3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YLF3"/>
    <dbReference type="OrthoDB" id="10576764at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007734">
        <property type="entry name" value="Heparan_SO4_2-O-STrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005331">
        <property type="entry name" value="Sulfotransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12129">
        <property type="entry name" value="HEPARAN SULFATE 2-O-SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12129:SF15">
        <property type="entry name" value="URONYL 2-SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03567">
        <property type="entry name" value="Sulfotransfer_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="15"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="151"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="151"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="182"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="218"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="235"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8024519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="620" mass="70636" checksum="DFBE04E1ABDD098F" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YLR6</accession>
    <name>A0A9Q0YLR6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Tenascin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_34778</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YLR6"/>
    <dbReference type="OrthoDB" id="126772at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00087">
        <property type="entry name" value="FReD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00104">
        <property type="entry name" value="KAZAL_FS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050373">
        <property type="entry name" value="Fibrinogen_C-term_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036058">
        <property type="entry name" value="Kazal_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040941">
        <property type="entry name" value="GGGWT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143">
        <property type="entry name" value="FIBRINOGEN/TENASCIN/ANGIOPOEITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07648">
        <property type="entry name" value="Kazal_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00186">
        <property type="entry name" value="FBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00280">
        <property type="entry name" value="KAZAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100895">
        <property type="entry name" value="Kazal-type serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51465">
        <property type="entry name" value="KAZAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="2" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040108884" evidence="4">
        <location>
            <begin position="17"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="Kazal-like" evidence="6">
        <location>
            <begin position="145"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="5">
        <location>
            <begin position="192"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8024772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="414" mass="44684" checksum="0D9DED9FB937AE6E" modified="2023-09-13" version="1">MKILVLCAILAYSARGGIVTADDHTGCEVTDAAIAELEELKTLVDQLTTLVETIIGGSIPEPGPFVIINPVLTVTWKEAKLTWTPPQGVIGYTATLITPEGVQPVVRLSPFTESFTTTDITVGEEYTVKITPIKGDGPGETFISEVFVPVCPMICTFEYRPQCGTDGTTYSNPCALEIAQCEGSDETLELAYEGPCVFADCSAALTNGKTTSGKYILQPAGAPAPFEAVCDMVTDGGGWTVILVRDSTGGNFYRDMADYVAGFGDLDGSHWLGLENLYLLNKQGNVEMLIQVTDQSGTSDVSTYSNFRINNADTDYTLETVAGFKGGAGNALFYNKGASFSARDMDNDGSVRNCAEELVGGWWYRGGMCSRSNPLGEYRTRAEFENEKGIFWAPFRGKTHSLSYISMKIRDPTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YM75</accession>
    <name>A0A9Q0YM75_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">V-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_36612</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8024011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YM75"/>
    <dbReference type="OrthoDB" id="10042941at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="CDD" id="cd15843">
        <property type="entry name" value="R-SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00957">
        <property type="entry name" value="Synaptobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50892">
        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="V-SNARE coiled-coil homology" evidence="3">
        <location>
            <begin position="228"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="19"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="19"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="49"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="73"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="84"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="121"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="162"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8024011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="311" mass="35445" checksum="B300426C4798D63B" modified="2023-09-13" version="1">MEVYAPFYGSVSSSQAINIPQHYTANGTKPSHGQSRHGHRNGGSLNQSKAKRGKKLKTMFRKLKKKNKKKKKDLTENGHHESVSRTSSYGSCSSSSSSQNEETSTSPFNPPQEYVTSCKEQPAKRMSKENRKQEQSSFNGGAESADLITSGKYNCADANSGIHEESDEWKKQSKSKKRKDGQKVYPKSQRLDSSTYEETQRNRVGRVKGIWRKIRKSKKKNKIREAGNLSQLYFKYEDVKGIMTTNSEKLLSRNGKLEDLQDRGEKLVKQCEVFSRHADQIKNEHASWWARMRDALRRLCCCFGCRCRAGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YN10</accession>
    <name>A0A9Q0YN10_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_33074</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YN10"/>
    <dbReference type="OrthoDB" id="10395319at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="192"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="203"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8025498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="333" mass="36643" checksum="D831A846F01D3C7C" modified="2023-09-13" version="1">MDKPPNGEKCLSLTGVFHPPRKISRPERYEVDDTPLSPPGSSSYLLFDSSTVFNTHPHKYRVLIGNNISSNKDESKDDLSVKHKPLGYRKSKSAVSLPSLDKESEATSREENHAPLSARAEQSSELFLKPKNLPNRKVSDTVVPGGTGLWSHFSSSTQYDHRRHSLATPGEDQLLENFKDAVTLNEEKPVSFQVEPFDEQKESMSASSGRGQETAGSLSTNEGAVAQQKYVVREIQPEPRRLFGFGPLYRPLPETVIEKQQPLTEMIQKEGLTAVGVDSTDSAAAASAATSQHKISEDGSKLTEVTPKTTKLETIFHDLRKILLRAFASLAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YN34</accession>
    <name>A0A9Q0YN34_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_33112</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YN34"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8025528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="88" mass="9843" checksum="CBC1A6A1AEF275BB" modified="2023-09-13" version="1">MPYIERKNPIVFGRGQRSPGVTGGQILKTLLTQYLEMRYGKRKNPIIFGGGERSSGVTGGQTLKTLLTRYLKKGSSDSSQTWYIDVPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YN52</accession>
    <name>A0A9Q0YN52_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Sulfotransferase family cytosolic 2B member 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_36151</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sulfotransferase 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YN52"/>
    <dbReference type="OrthoDB" id="205623at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11783">
        <property type="entry name" value="SULFOTRANSFERASE SULT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sulfotransferase" evidence="3">
        <location>
            <begin position="46"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8023654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="315" mass="36736" checksum="9E333BBC33A7908B" modified="2023-09-13" version="1">MPLKMARDAKRFYQQYSFMYKDVLPLPIVTNENAMKEFELMELREDDLFVVTYPKSGTHWMQEIVHLILVNGHGERLTAKERRPVIELADIRSHDPELVAAAGPVLREMKDVPSPRVLTSHVQYSFLPKQFLEKRPKIIYIYRHPKDVLVSLFNFYLSFIRQDVEPAPFGDFFDDFTAGQVVFGSWFDHVNDYYKHKDDENFFALPFEAMKQDLHKVVQDVASFIGKPLDDNSLARVVEGASFETMKKSFQKDHEKNVAEGKDVMRANAFVRKGKVGQWKDLLTPAQNKLLDELFREKMKDCEWTRSYAPDSANE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YNB0</accession>
    <name>A0A9Q0YNB0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">BTB/POZ domain-containing protein KCTD9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_33159</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YNB0"/>
    <dbReference type="OrthoDB" id="9989223at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18368">
        <property type="entry name" value="BTB_POZ_KCTD9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17073">
        <property type="entry name" value="KHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.80:FF:000002">
        <property type="entry name" value="Potassium channel tetramerization domain-containing 9a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.80">
        <property type="entry name" value="E3 ubiquitin-protein ligase SopA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001646">
        <property type="entry name" value="5peptide_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021789">
        <property type="entry name" value="KHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051082">
        <property type="entry name" value="Pentapeptide-BTB/POZ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14136">
        <property type="entry name" value="BTB_POZ DOMAIN-CONTAINING PROTEIN KCTD9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14136:SF17">
        <property type="entry name" value="BTB_POZ DOMAIN-CONTAINING PROTEIN KCTD9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11834">
        <property type="entry name" value="KHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00805">
        <property type="entry name" value="Pentapeptide"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141571">
        <property type="entry name" value="Pentapeptide repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51490">
        <property type="entry name" value="KHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KHA" evidence="1">
        <location>
            <begin position="41"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8025567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="437" mass="48408" checksum="A3033C63D9A27ABC" modified="2023-09-13" version="1">MHSLSRERRKEESHSYSFFGHLGTFFSCTLYVNNSDIMKQRVTLLRNGKTTEGKVILLPDSRDKLLVEASAALGINATVIYNKYGGRIEDFDLIRDDDILYMSEGEPFIDPKYSNSDESSSTISNNSVIQRNPQIQWVTLNVGGTIFTTTRSTLCGRDSDSMLARMFGTEGPSWSSAVDHSGAYLIDRSPKYFEPILNYLRHGQLIVDTGVSAEGVLEEAKFFGLTSLLEPLKQMVKERKPPGDYTPITRREFITMLKGCALNSELRCQGMNFKGADLAKLDLRFINFKMADLSHANLSGANMAFCSLERTNLSFAKLDQANLQGVKMVLANLEGASLRGCNFEDPAGSKANMEGVNMKGVNLENSQMAGVNLRVAKLKDANLQNSNLRRAVLAGTDLENCNLTGCDLQEANLRGANTKNAKFEDMITPLHMSQSVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YNM1</accession>
    <name>A0A9Q0YNM1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_36483</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YNM1"/>
    <dbReference type="OrthoDB" id="6022531at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008528">
        <property type="term" value="F:G protein-coupled peptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009755">
        <property type="term" value="P:hormone-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24372:SF77">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24372">
        <property type="entry name" value="GLYCOPROTEIN HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="48"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="91"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="136"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="178"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="254"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="296"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="334"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="9">
        <location>
            <begin position="67"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8023909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="372" mass="41802" checksum="9FCF843FFBC26DB5" modified="2023-09-13" version="1">MFSKPEIKKYPLGLVRITDDPFICCYLNKSDTTCNTPPSPFSTCEGIFYSWLVRVSAWIVALVALVTNLMVLLGRARRKLPNNRKPAENIFLANLAVADILMGVYLFAIAIADAWFGADYYRYSQKWRTSPICGAIGVIGIVSSIVSMLDLTIITIDRFLCIVYPFGNIRFNRKWSKIVCAVIWLAGLAIALLPIFLKKIVSNFYGYTDVCLGLPVVRIPRGVAADYKAAGLKYKIKEYSETEERVTWLYSSILYTYFGSTCLFVVAVCYICMFISVLRSRRDSHRPSHGNDEMKLATTISVIVGTDMLCWLPIITLSVLSQSGIDIPTKVNPWLVVFVIPINSAMNPFIYSYGILKRRKKNAPSAIWQGPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YPM2</accession>
    <name>A0A9Q0YPM2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Septin-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_34020</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8026227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YPM2"/>
    <dbReference type="OrthoDB" id="8954335at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="CDD" id="cd00882">
        <property type="entry name" value="Ras_like_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32046:SF14">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32046">
        <property type="entry name" value="G DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="241"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="252"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8026227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="551" mass="63098" checksum="5A10229D6190BDB2" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YRR3</accession>
    <name>A0A9Q0YRR3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetraspanin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_36856</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the tetraspanin (TM4SF) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8024196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YRR3"/>
    <dbReference type="OrthoDB" id="438211at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03127">
        <property type="entry name" value="tetraspanin_LEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1450.10">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000301">
        <property type="entry name" value="Tetraspanin_animals"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF519">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002419">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00259">
        <property type="entry name" value="TMFOUR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="54"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="87"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="207"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8024196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="239" mass="25658" checksum="3B0940154B661784" modified="2023-09-13" version="1">MVAGCGPKLAKLLLFIVNFSVWAGSLALIGLGIWMLVESSRFDTLFAEDNIKPIAGIILGLGCFLFIVGFCGCCGAMKESTCYLNMYFTFLLLIVLGELTAGILVFVYSDEIESSMTRGMTSTIKNDYGVTDAATESVDYFQKEFECCGASNYTDYSGSKFEEDQGVAGALPDTCCPKDTVTCSVTSDAQYKEGCVSASLDKLQSNYAIVGGICLGILAIEICAMIFSCCMVNAIKEDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YSZ1</accession>
    <name>A0A9Q0YSZ1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">2-hydroxyacylsphingosine 1-beta-galactosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_33881</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8026127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YSZ1"/>
    <dbReference type="OrthoDB" id="5835829at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008194">
        <property type="term" value="F:UDP-glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050271">
        <property type="entry name" value="UDP-glycosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043">
        <property type="entry name" value="EG:EG0003.4 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043:SF145">
        <property type="entry name" value="FI06409P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="825"/>
            <end position="851"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8026127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="859" mass="96664" checksum="2121B759B2879B26" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YT63</accession>
    <name>A0A9Q0YT63_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_35519</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8025336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YT63"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8025336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="74" mass="8421" checksum="A83C0FE35CF8DACD" modified="2023-09-13" version="1">MLYRELGRGPLVTGIFLYQVQGNGTTFLQTCSRAVPSPFLGKSSRPTCSERLFSSSSSLFIFIFFFQFMYVFVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1B9V7</accession>
    <name>A0A9Q1B9V7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_41797</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8019986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1B9V7"/>
    <dbReference type="OrthoDB" id="10012494at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040247">
        <property type="entry name" value="DUF5524"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31097:SF2">
        <property type="entry name" value="CHROMOSOME 7 OPEN READING FRAME 57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31097">
        <property type="entry name" value="SI:DKEY-276J7.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17662">
        <property type="entry name" value="DUF5524"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="126"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="177"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="247"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="287"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="132"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="177"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="254"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="287"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="312"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="340"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="357"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8019986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="366" mass="42274" checksum="1B180C9D60C4ED46" modified="2023-09-13" version="1">MVTASTTLPVRFENLLLIFVAFGETISKGREKNPKFLKQKCKKKKKKKRSLLCVMAGNSGTQDWYYHAPAKKENKGPVDYPAASQIPLIGGDFIAPDRDDEGMKRGWLRDSDSKYVRLAKLGGRKDLLSMRSPPPKPDAPKPYPRVDWFDHHVEEEPVDTSPQKVYLPDYMVHEEYTPEKPQEDELSGKRQPPRRPPYSLQDNISAFQRDGDSVTDKTMKLDEDVPYSKRFAKKDKKRDVEQMNKALAARRDSSGQNQKNTIMQEPTEPVIFGKLLSMGYQRDWNDQREKYYDQQRSFKEKQPSYKTQAEANKNKDPVTTEYREEINKKGKQLQTIKAGKRSDTKSKLKQSIKDENSNNMLKTKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BA48</accession>
    <name>A0A9Q1BA48_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_44548</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000980">
        <property type="protein sequence ID" value="KAJ8017808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BA48"/>
    <dbReference type="OrthoDB" id="10067103at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46954">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46954:SF1">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="516"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8017808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="538" mass="60943" checksum="64544ACB7CC4F5A2" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BAU7</accession>
    <name>A0A9Q1BAU7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_40885</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8021111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BAU7"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="118"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="139"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="165"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8021111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="330" mass="40365" checksum="3E64D8721BA08C0E" modified="2023-09-13" version="1">MNDRKNERKEDKEADRQAGRKVDSKKEERKKKERKEERTKGKKKDRKKKRERERKKKEGKKEKKKDKKKRKKGSKEGRKKERKKETESKKERRKEKEKKRKKERKKERKKERKQASKQARKKASKQARKKERKKEEKKERKKERKKERKKERKKERKKERKKERKKEGRKEGRKEGRKEGRKEGRKEGRKEGRKEGRKEGRKEGRKEGRKEGRKEGRKEGRKEGRKEGRKEGRKEGRKEGRKEGRKDTNKKFLLTFPTILFTKFRSNANKNDPTTLTGMTALSNEKQKYLGIFCRKEMLCRNELYPSKLPLWRYHTEFALLFPQNTFVLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BBI1</accession>
    <name>A0A9Q1BBI1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_43553</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000358">
        <property type="protein sequence ID" value="KAJ8018452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BBI1"/>
    <dbReference type="OrthoDB" id="5958414at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1820">
        <property type="entry name" value="L1 transposable element, RRM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35555">
        <property type="entry name" value="ENDONUCLEASE-REVERSE TRANSCRIPTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35555:SF3">
        <property type="entry name" value="ENDONUCLEASE-REVERSE TRANSCRIPTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="84"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8018452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="519" mass="60665" checksum="9F3C77B54A6CE8AD" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BC28</accession>
    <name>A0A9Q1BC28_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Zinc finger PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_39637</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8022221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BC28"/>
    <dbReference type="OrthoDB" id="5795902at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Zinc finger PHD-type" evidence="4">
        <location>
            <begin position="26"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8022221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="206" mass="23864" checksum="03EBCEC8046924FD" modified="2023-09-13" version="1">MLPFGIKGSNRVARPKRISLIREGIFCTCRRPDDGKKMAECTFCKEWFHEACEGPITRRNFYCCSFCSRRFDKRKVAASNDRMYGVDSYRALQRFKKRYGIHKWHDEAVRMYKFVNDTVLDGAFPAADGKLAVTEKLPEHLGANIKGITITRPDDPSVGFSFIYIQGPKHLTLLSLYETPSFMEWRTQNTTWMVVSHLLIIKHTRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BCX3</accession>
    <name>A0A9Q1BCX3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_39377</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8022010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BCX3"/>
    <dbReference type="OrthoDB" id="10037236at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47510:SF3">
        <property type="entry name" value="ENDO_EXONUCLEASE_PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47510">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8022010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="166" mass="19145" checksum="7A55109C43E99B89" modified="2023-09-13" version="1">MSPRYRPLVKRIKPNVIEVNRWADDAVSKLQGCLESTDWSVFVDANPELSDLVDVVSSYIQFCRENSIPTKKVKVSPNNKPWITKEVKSVINRKKQLYGRGEKDKLNAVQKEIARVIKVEKAKYRDTLEKKLAKNYIKQVWEGMRLMSGYSNNCKKHSDLPCTSAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BD96</accession>
    <name>A0A9Q1BD96_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Integrator complex subunit 9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_40699</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. INTS9 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BD96"/>
    <dbReference type="OrthoDB" id="5600060at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032039">
        <property type="term" value="C:integrator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034472">
        <property type="term" value="P:snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022712">
        <property type="entry name" value="Beta_Casp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027074">
        <property type="entry name" value="Integrator_9su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048660">
        <property type="entry name" value="IntS9-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46094">
        <property type="entry name" value="INTEGRATOR COMPLEX SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46094:SF1">
        <property type="entry name" value="INTEGRATOR COMPLEX SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10996">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21382">
        <property type="entry name" value="IntS9_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16661">
        <property type="entry name" value="Lactamase_B_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01027">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Beta-Casp" evidence="6">
        <location>
            <begin position="308"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8020959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="671" mass="75199" checksum="DDD9ACBEA7D9EC82" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BDT0</accession>
    <name>A0A9Q1BDT0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_38987</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BDT0"/>
    <dbReference type="OrthoDB" id="2113965at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8021708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="174" mass="19110" checksum="46394FFB70D1CB66" modified="2023-09-13" version="1">MPHIERKNPIVFGGGQRSPGVTGGQNLKTLLTQYLKMRYGKRKNPIISGGGQRSPGITRGQNLKTLLTQYLELCYGKRKNPIIFGRGQRSSGVTGGQTLKTSLTRYLKMRYGKRKNPIIFGRGQRSSGVTGGQTLKTLLTRYLKMCYGMRKNPIIFGGGQRSSGVTGGQSLKTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BE35</accession>
    <name>A0A9Q1BE35_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_34297</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BE35"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8024396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="91" mass="10880" checksum="AEE4896B1CD38588" modified="2023-09-13" version="1">MEKMDIGQSDTFQNFVKDEWVIDATEDFLCNTMDVSTLKTWLGKLRKGWLQSPKRVWDALKEKDIEQTIRTRLEGRFIPRWMGKVNYHTVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BEI7</accession>
    <name>A0A9Q1BEI7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">G-protein coupled receptor moody</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_38968</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BEI7"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00637">
        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24228">
        <property type="entry name" value="B2 BRADYKININ RECEPTOR/ANGIOTENSIN II RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24228:SF72">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="89"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="131"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="176"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="266"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="292"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="29"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="222"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="222"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8021692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="366" mass="40220" checksum="5E1B72F30AB600AD" modified="2023-09-13" version="1">MAYKFDDYPAQQIIVAILACIAAITGLFGNVLVLVAIGLSRKLHTSTNAFVTSLAACNLLTCSTMPYHVNTILSDPPKNLGVCRFFGSVLISTQIISLITLSLIAVLRAFQMRVHRWIGISTFDRVYTKRNIGVMIAFSWIVPFLAVFVSSVARWATLGYNEKYNICIIEASKSGMASLVAGIVVGLPSLVIIVACYSLIFFHMHNYHAELKLKLVSANPTDNNASHQHSTTPDDNKPTPKRKVKNNQSSAEQSISEREHKINRTLFIVVCAFLISVLPFSIAIISPNINAAIPWCAVLLMSNSAMNPIIYGLHHPEFHKVFQNYFTCKICRGVRMIPSTFGTLTSNVNSSPGANTMQETDTTSCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BHW3</accession>
    <name>A0A9Q1BHW3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Neuropeptide F receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_33058</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BHW3"/>
    <dbReference type="OrthoDB" id="9046662at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042923">
        <property type="term" value="F:neuropeptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004983">
        <property type="term" value="F:neuropeptide Y receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000611">
        <property type="entry name" value="NPY_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24235:SF29">
        <property type="entry name" value="GH23382P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24235">
        <property type="entry name" value="NEUROPEPTIDE Y RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01012">
        <property type="entry name" value="NRPEPTIDEYR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="38"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="74"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="105"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="151"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="201"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="252"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="290"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="53"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="362"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="362"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8025487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="390" mass="44119" checksum="01063934E28CA82A" modified="2023-09-13" version="1">MEDLTTQVDTIFEFTTMSGNTTLPECNLIWFHPLVKTLLIGIVALLAILGIFGNVLVIIVVARRPKMRTVINWFITNLSLTDLLYCTVGIPVTVTSLFAKNWPLGLVFCGVAQMITSCLVLVSSLTLTAIAVDRFCLVVYPFLKPVNRNRCFAIIIVIWIFCVGLTLPMSIYAKVEDITEIVQYERIVCIGGMNETVSIKYFTAIFTIQFCYPMILVTIAHVSIAWKLKRNVKPGVRRKDTEQRENTRRQRMNRMLSAVVIVFASCWLPLNLYDMFYDYRIINPTEICVVPFIIFLPSVCSTFLNPLLYAWLNDNFRKEFYKLLPFLTILGEASYKSSAGPTGSHASGAFSNLKRPRTTLGSYLQPTTAESTVLKTTPRPQRKGVEDTKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BIB5</accession>
    <name>A0A9Q1BIB5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_31205</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BIB5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8026402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="87" mass="10197" checksum="505EC99C271E43B7" modified="2023-09-13" version="1">MPFNLDKFVASPSVEELDSLKKSEIVKVAKHYGIEFQPLMRKEEIKRYVLEYLVDEGVLPSTVLENSYQLCQTDNTFELKRLEIENE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BIB7</accession>
    <name>A0A9Q1BIB7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Retroviral-like aspartic protease 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_31224</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BIB7"/>
    <dbReference type="OrthoDB" id="6759373at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45823">
        <property type="entry name" value="T-SNARE COILED-COIL HOMOLOGY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45823:SF1">
        <property type="entry name" value="T-SNARE COILED-COIL HOMOLOGY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="216"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="121"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="146"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="168"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8026417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="401" mass="45411" checksum="4B5497CDB593A669" modified="2023-09-13" version="1">MASNSFSSSKSPGVHPDVTGNLMRQVESLTRECNRLMRETRKEKKENFQNFSMDDFLELPDKVTSRKYQKGEEEIIPVARKPSEGLEFPSESFSHASGQNQLDSVPSYARRDRSDSYGARHGSDSVLKAEEEQDRDIFTETLLKGNHTDPLSEPRSKSRVTFSPTLEGELKNPGRRKGEFQDLGNVQFDREEEHPSEFFHSRGVRDPADHEYRRRRAENPANSLPSHTGLHEHGTRPKPLMLPEKFDGSQPWEDYLTHFKSVAEINGWNREQKARFLGANLKGGALEVYTEISSAARTSFSAVVEALERRFGRAHQKPLFLAQLSMRRRGRNESLQQLAQAIKRLVNGAYPSLNMNARDGIAVESFRAALDDVELQRAVFMAKPDSLAEAVTAAVEMESFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BJF7</accession>
    <name>A0A9Q1BJF7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">RNA-directed DNA polymerase from mobile element jockey</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_29768</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8027731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BJF7"/>
    <dbReference type="OrthoDB" id="416454at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061343">
        <property type="term" value="P:cell adhesion involved in heart morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007508">
        <property type="term" value="P:larval heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33395:SF22">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33395">
        <property type="entry name" value="TRANSCRIPTASE, PUTATIVE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="468"/>
            <end position="719"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8027731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="747" mass="83723" checksum="9DB6D8CFEBAEA16C" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BJR4</accession>
    <name>A0A9Q1BJR4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Fibropellin-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">HOLleu_29801</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">Nanhai2018</strain>
            <tissue evidence="20">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8027755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10066368at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005112">
        <property type="term" value="F:Notch binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000246">
        <property type="entry name" value="EGF-like repeat and discoidin I-like domain-containing protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000031">
        <property type="entry name" value="neurogenic locus notch homolog protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000327">
        <property type="entry name" value="neurogenic locus notch homolog protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000173">
        <property type="entry name" value="Neurogenic locus notch protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000143">
        <property type="entry name" value="Protein crumbs 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000321">
        <property type="entry name" value="Protein delta homolog 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000146">
        <property type="entry name" value="Putative neurogenic locus notch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.10:FF:000018">
        <property type="entry name" value="Sushi, von Willebrand factor type A, EGF and pentraxin domain-containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000002">
        <property type="entry name" value="Unc-5 netrin receptor C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000472">
        <property type="entry name" value="Uncharacterized protein, isoform A"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="23"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.10">
        <property type="entry name" value="Tumor Necrosis Factor Receptor, subunit A, domain 2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013032">
        <property type="entry name" value="EGF-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003410">
        <property type="entry name" value="HYR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006558">
        <property type="entry name" value="LamG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001791">
        <property type="entry name" value="Laminin_G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001759">
        <property type="entry name" value="PTX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011641">
        <property type="entry name" value="Tyr-kin_ephrin_A/B_rcpt-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12916">
        <property type="entry name" value="CYTOCHROME C OXIDASE POLYPEPTIDE VIC-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12916:SF4">
        <property type="entry name" value="UNINFLATABLE, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07699">
        <property type="entry name" value="Ephrin_rec_like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12661">
        <property type="entry name" value="hEGF"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02494">
        <property type="entry name" value="HYR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13385">
        <property type="entry name" value="Laminin_G_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00090">
        <property type="entry name" value="TSP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00010">
        <property type="entry name" value="EGFBLOOD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="27"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="21"/>
    </dbReference>
    <dbReference type="SMART" id="SM01411">
        <property type="entry name" value="Ephrin_rec_like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00282">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00560">
        <property type="entry name" value="LamGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00159">
        <property type="entry name" value="PTX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="21"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="23"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50825">
        <property type="entry name" value="HYR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50025">
        <property type="entry name" value="LAM_G_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51828">
        <property type="entry name" value="PTX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="10 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 12" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0914">Notch signaling pathway</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="13" id="KW-0768">Sushi</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Sushi" evidence="18">
        <location>
            <begin position="63"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="18">
        <location>
            <begin position="130"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="18">
        <location>
            <begin position="214"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="HYR" evidence="17">
        <location>
            <begin position="267"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="18">
        <location>
            <begin position="359"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="18">
        <location>
            <begin position="440"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="18">
        <location>
            <begin position="503"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="domain" description="HYR" evidence="17">
        <location>
            <begin position="566"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="18">
        <location>
            <begin position="654"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="18">
        <location>
            <begin position="715"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="domain" description="HYR" evidence="17">
        <location>
            <begin position="842"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="18">
        <location>
            <begin position="924"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="domain" description="HYR" evidence="17">
        <location>
            <begin position="1059"/>
            <end position="1144"/>
        </location>
    </feature>
    <feature type="domain" description="HYR" evidence="17">
        <location>
            <begin position="1145"/>
            <end position="1227"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="1676"/>
            <end position="1712"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="1714"/>
            <end position="1750"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="1752"/>
            <end position="1788"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="1790"/>
            <end position="1826"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="1828"/>
            <end position="1864"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="1866"/>
            <end position="1902"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="1909"/>
            <end position="1947"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="1949"/>
            <end position="1985"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="1987"/>
            <end position="2023"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="2025"/>
            <end position="2061"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="2063"/>
            <end position="2099"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="2101"/>
            <end position="2136"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="2156"/>
            <end position="2191"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="2193"/>
            <end position="2229"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="2231"/>
            <end position="2265"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="2267"/>
            <end position="2302"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
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        <location>
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            <end position="2704"/>
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        <location>
            <begin position="2506"/>
            <end position="2674"/>
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        <location>
            <begin position="100"/>
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    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="239"/>
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        </location>
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    <feature type="disulfide bond" evidence="12">
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        <location>
            <begin position="2770"/>
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            <dbReference type="ARBA" id="ARBA00004479"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
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        <source>
            <dbReference type="SAM" id="Coils"/>
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        </source>
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            <dbReference type="PROSITE" id="PS51828"/>
        </source>
    </evidence>
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            <dbReference type="EMBL" id="KAJ8027755.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="3143" mass="342742" checksum="7734E868B9B939C6" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BJZ0</accession>
    <name>A0A9Q1BJZ0_HOLLE</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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    </gene>
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        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
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        <property type="molecule type" value="Genomic_DNA"/>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8028030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="214" mass="24403" checksum="6248C9203F8857BB" modified="2023-09-13" version="1">MYSLVLLRRTFTHGAACRIPSDLSRSCGYFPTAESNASVRITQCYKIGLNHAFISGYVSWTTSSFPIRSYYTTRPNWPRRSFVCSGKPGGRRPSGRGFQIVNHESCSSLGLTIHSLNCKIQICQQLLQRFLSTDTGPGIPKQTLIMEGTIDRFNGVRIDMEEQLRRCETLTAEGKWNLKESLLRKCVEHFHITKQRINLQCGWKYLLSVGILYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BKF5</accession>
    <name>A0A9Q1BKF5_HOLLE</name>
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        <recommendedName>
            <fullName evidence="1">Thioredoxin domain-containing protein 9</fullName>
        </recommendedName>
    </protein>
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        <name evidence="3" type="ORF">HOLleu_30405</name>
    </gene>
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        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
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            <taxon>Metazoa</taxon>
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            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome 15"/>
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        <property type="entry name" value="Thioredoxin-like_sf"/>
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        <property type="entry name" value="Thioredoxin_domain"/>
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        <property type="entry name" value="THIOREDOXIN DOMAIN-CONTAINING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="2">
        <location>
            <begin position="75"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00026148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8028224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="158" mass="18351" checksum="4340D6F94D4FA25F" modified="2023-09-13" version="1">MDQAGIENAVQQQVLKATEMIESQLDAEIEKLDHMDADEMEILRQRRLDAMKKSQNQKQEWLAIGHGTYTEVPGEKEFFTECKKSKKVVCHFYRDSTMRCKIVDKHMEILARKHIETRFLKINAEKSPFLTERLNITVLPTLALILDGKTKDYVVGIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BLT3</accession>
    <name>A0A9Q1BLT3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Polyubiquitin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_28320</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ubiquitin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BLT3"/>
    <dbReference type="OrthoDB" id="428577at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd01803">
        <property type="entry name" value="Ubl_ubiquitin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000158">
        <property type="entry name" value="Polyubiquitin 5"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019954">
        <property type="entry name" value="Ubiquitin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019956">
        <property type="entry name" value="Ubiquitin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050158">
        <property type="entry name" value="Ubiquitin_ubiquitin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10666">
        <property type="entry name" value="UBIQUITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00348">
        <property type="entry name" value="UBIQUITIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00299">
        <property type="entry name" value="UBIQUITIN_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="8">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="8">
        <location>
            <begin position="77"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="8">
        <location>
            <begin position="153"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="8">
        <location>
            <begin position="229"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="8">
        <location>
            <begin position="305"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="8">
        <location>
            <begin position="381"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8029032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="457" mass="51508" checksum="74149C31417AD7D2" modified="2023-09-13" version="1">MQIFVKTLTGKTITLEVEPSDTIENVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BMJ2</accession>
    <name>A0A9Q1BMJ2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Tropomyosin alpha-1 chain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_28800</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the tropomyosin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BMJ2"/>
    <dbReference type="SMR" id="A0A9Q1BMJ2"/>
    <dbReference type="OrthoDB" id="128924at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000001">
        <property type="entry name" value="Tropomyosin alpha-1 chain isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000001">
        <property type="entry name" value="Tropomyosin alpha-1 chain isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000533">
        <property type="entry name" value="Tropomyosin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19269">
        <property type="entry name" value="TROPOMYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00261">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00194">
        <property type="entry name" value="TROPOMYOSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="99"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="162"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="12"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="108"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="122"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8029417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="284" mass="33196" checksum="62D7841FA445708E" modified="2023-09-13" version="1">MDTIKKKLSQLKADKEKALDEKDAAEAAMKEALEREEQVNDENKELQTRIKQLESELDDTSEKLNATVVKCEEAEKAQQTAENEMANLQRKLQLTEEELSRAEERVNDLQSKYNDIEQNSEESERQRKVLESRSAADDERMEEIKTTLKGIQESSLETEKLYAEASRKLTVTEEELQRSEERSADLESQLNQMKEELHQLHNNLKSMEAREEKYNENEEMYEAKIKTLEDKLKEAENRADFAEESLKSLKTAVEQLEGDLEKEKEQHEALKEEYRQVMSEMENV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BPB9</accession>
    <name>A0A9Q1BPB9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_26434</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BPB9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31025">
        <property type="entry name" value="SI:CH211-196P9.1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31025:SF25">
        <property type="entry name" value="ZINC FINGER (C2H2)-60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="128"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="137"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8030124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="242" mass="27148" checksum="8BFE625797BB000F" modified="2023-09-13" version="1">MAFACHFTYGDTKKIVKFPNHLPDNIISEIRGAFGIEVFDFPNPLKLQKYEEDWKDWVDVTPLELQQGMKIKVVPSNAPVFSPMEEVHSPGSASTILLESNSTSCDEDLDSMSMPIFELPLVGCSDSQQSFSGDNVSPRTSSPQPPTTSATAVMSPQSSDKDDDQSVWPKKFELPPLPRRLAEKLKKGIFPDEKERRLIISCVFNEMAGFVRYPTSAQYNEVAALLAQKFPVLRRILAQHHM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BRV3</accession>
    <name>A0A9Q1BRV3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Acetylcholinesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_24715</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the type-B carboxylesterase/lipase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BRV3"/>
    <dbReference type="OrthoDB" id="19653at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003990">
        <property type="term" value="F:acetylcholinesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006581">
        <property type="term" value="P:acetylcholine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019695">
        <property type="term" value="P:choline metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050654">
        <property type="entry name" value="AChE-related_enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43918">
        <property type="entry name" value="ACETYLCHOLINESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43918:SF4">
        <property type="entry name" value="CARBOXYLIC ESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0719">Serine esterase</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040287577" evidence="5">
        <location>
            <begin position="46"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="domain" description="Carboxylesterase type B" evidence="6">
        <location>
            <begin position="49"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="588"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="590"/>
            <end position="602"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8031505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="620" mass="69709" checksum="70D9A2E9AD3224AA" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BST9</accession>
    <name>A0A9Q1BST9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Galactosylceramide sulfotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_26074</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the galactose-3-O-sulfotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BST9"/>
    <dbReference type="OrthoDB" id="514299at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001733">
        <property type="term" value="F:galactosylceramide sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009247">
        <property type="term" value="P:glycolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009729">
        <property type="entry name" value="Gal-3-0_sulfotransfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14647">
        <property type="entry name" value="GALACTOSE-3-O-SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14647:SF86">
        <property type="entry name" value="GALACTOSE-3-O-SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06990">
        <property type="entry name" value="Gal-3-0_sulfotr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="9"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8032523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="497" mass="58220" checksum="6B7C8272F7818C1A" modified="2023-09-13" version="1">MAKVMWQSFCFLSLVVITSVVVISWHLFNNGDRDPSVHRHVHLVHTDRLDRNSKRGNPPDLLFHWDVDKRSDYDAVMDFMEQGVAGHPQNQRGDIAARKLDLNSDSEPDSTDDENWTKNKLQVDNLPGEENVKKVNVSESLVPKEVGTSRLRADVAKLYNVTSTCKPLQSIAMIKTHKCSSSTLQNILFRKAEDLDLQVALPATSVYMGSPSYFSRRYIHLDRGQKYNIMASHMRFRSPRDVKQVMMDDAKYITILRDPVKQYESIFTYYGLQKRFKARLHDFLKSPERHFRLFEPRMTRVVGRNPMCYDNGWDARLFPEGNTRIDQFITILEEWYDLVLIAEYFDESLILLKDLMCWTLDDVVYFSQNARNKDSVVSLSSEDKVAIHKWNWADSKLYVHFNKSLWQKIEQYGKERMELEVNILTQKKEKLAADCIGQTVKEGDKRMWYPSGIKVNSFLLNPNAVHKHLCEQMTRPENTFLNKLREKQMMSFRRGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BTK0</accession>
    <name>A0A9Q1BTK0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Mitochondrial carrier-like 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_25922</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BTK0"/>
    <dbReference type="OrthoDB" id="10253709at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10780:SF18">
        <property type="entry name" value="LD43650P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10780">
        <property type="entry name" value="MITOCHONDRIAL CARRIER HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="2"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="121"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8032405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="308" mass="34426" checksum="861B4AB7EF770740" modified="2023-09-13" version="1">MATGPDPWTQLAVNVAITTFTHPMGYVKTLIQVGYEPIAPRVGKNMFFRKTLLLPGFFQYAGHIKKIDGFFGLYRGVTPRILASISGSIVARVTTQKLDEHFPSPQEEATESKKTGKVSTQEFLRESAKLTVSHCCGAVVSHPFQVVAVRAMVQFIGKETKYSGFLNPYREVYNENGIWGFFAGLVPRVLMDVFTLWLARTMFHIFNNYILDENMSNAKEIRNYMHAITGFVAGMVTYPLGLVSTLMCVNDSGLMAGSPPFTPLYPNWRSCWRDLSERGLLKRGSSLFFRPYQAGAVAIEAPILTPLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BTP5</accession>
    <name>A0A9Q1BTP5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Reverse transcriptase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_23094</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8032988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10064731at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09274">
        <property type="entry name" value="RNase_HI_RT_Ty3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000115">
        <property type="entry name" value="Polyprotein of retroviral origin, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000032">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 297-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054465">
        <property type="entry name" value="Integrase_p58-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041373">
        <property type="entry name" value="RT_RNaseH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22938">
        <property type="entry name" value="Integrase_p58_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17917">
        <property type="entry name" value="RT_RNaseH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="10">
        <location>
            <begin position="757"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase" evidence="9">
        <location>
            <begin position="1185"/>
            <end position="1362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="316"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="581"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="680"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="78"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="316"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="355"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="581"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="624"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="649"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="696"/>
            <end position="720"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8032988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1577" mass="180059" checksum="BD16C9B605DBE14B" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BVI5</accession>
    <name>A0A9Q1BVI5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Solute carrier family 35 member G1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_23523</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BVI5"/>
    <dbReference type="OrthoDB" id="306876at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22911">
        <property type="entry name" value="ACYL-MALONYL CONDENSING ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22911:SF6">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER G1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="25"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="88"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="113"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="141"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="174"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="207"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="239"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="266"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="292"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="24"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="322"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="338"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8033321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="353" mass="37959" checksum="56E8FE4A2EF1C68F" modified="2023-09-13" version="1">MEEGEVLGNFNCFKRAFLSIYHHRGILMAFAAAFCSVSSTLTVSSLEGAIGPTEVTFMRGLVTATLSLVLLVRRGVRIRPSSLGELKLLVLHSLLGAVGIWTQYYAYQNMATADAAAIIFGYIAFTALFARIFLKEPFGWLEGLLIVLTITGVVFIMQPPFLFGGYGSEKSSTILPALAAFCTCLLGGFQMVALRQLGKQGTDACKTVMYMGIALMVCTAISIASGGPWSVPGCSFTRFLSVLVGLFSFTSFSLLSFALTLENAVVISLITVSQVYLIFIADILFFGAKPDWFSITGTVLIVGSSMANSLKTVFGKKRMSATEDSKHEVDESVEGADTSPSENMESTVIESTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BWC6</accession>
    <name>A0A9Q1BWC6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ryncolin-4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_24470</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8034052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BWC6"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00087">
        <property type="entry name" value="FReD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050373">
        <property type="entry name" value="Fibrinogen_C-term_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025615">
        <property type="entry name" value="TILa_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040941">
        <property type="entry name" value="GGGWT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143:SF459">
        <property type="entry name" value="FIBRINOGEN C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143">
        <property type="entry name" value="FIBRINOGEN/TENASCIN/ANGIOPOEITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12714">
        <property type="entry name" value="TILa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00186">
        <property type="entry name" value="FBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040216210" evidence="1">
        <location>
            <begin position="36"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="2">
        <location>
            <begin position="94"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8034052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="333" mass="36961" checksum="8FD72FD76723AA48" modified="2023-09-13" version="1">MFSRKANTPSVRRNHLCTVCPLILVGVLCSTLVEGCTFVNTDCTIRCVCRNDQISCNTNYKCSPYAECREDDGIRGCYCKEGYKSNGASCTPVTPATPIYTDCYEAQMAGNTVDGVYTVSPPDWPGVPFPVFCDMTTDGGGWTVFQRRVDGVSNFYRDWTAYKNGFGSTNNGSDYWLGNEQLHYLTKAKTYRLRVDITKSDGSTLYAEYASFSISSEDLKYKLKISTLTAGNAGDGLYAYDNGGYFTTYDRDNDGCSYRNCAEGVGGAGWYTNSWCYECSAGGPACSYYGTNSCRNWCSYFNLNGIYNGENGQNIYWSSTCNLKYVNMKIRPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BWW2</accession>
    <name>A0A9Q1BWW2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Retrovirus-related Pol polyprotein from transposon</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_20966</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BWW2"/>
    <dbReference type="OrthoDB" id="775972at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00303">
        <property type="entry name" value="retropepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.10.10:FF:000002">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 17.6-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.10.10:FF:000007">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 17.6-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001995">
        <property type="entry name" value="Peptidase_A2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053134">
        <property type="entry name" value="RNA-dir_DNA_polymerase"/>
    </dbReference>
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        <property type="entry name" value="RT_dom"/>
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        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24559">
        <property type="entry name" value="TRANSPOSON TY3-I GAG-POL POLYPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13650">
        <property type="entry name" value="Asp_protease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50175">
        <property type="entry name" value="ASP_PROT_RETROV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="3" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
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        <location>
            <begin position="99"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase" evidence="10">
        <location>
            <begin position="412"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="597"/>
            <end position="618"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8034222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="618" mass="68792" checksum="940E3BA42BC1B250" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BXR3</accession>
    <name>A0A9Q1BXR3_HOLLE</name>
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        <submittedName>
            <fullName evidence="5">DDB1- and CUL4-associated factor 8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_21659</name>
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    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BXR3"/>
    <dbReference type="OrthoDB" id="4869960at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080008">
        <property type="term" value="C:Cul4-RING E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
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    </dbReference>
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        <property type="entry name" value="DCAF8"/>
    </dbReference>
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        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15574:SF21">
        <property type="entry name" value="DDB1- AND CUL4-ASSOCIATED FACTOR 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15574">
        <property type="entry name" value="WD REPEAT DOMAIN-CONTAINING FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="260"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="27"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="592"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="626"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="28"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="63"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="73"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="105"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="131"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="158"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="173"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="596"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="635"/>
            <end position="655"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8034705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="664" mass="75065" checksum="6D8B4D5B20215A7B" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BXW4</accession>
    <name>A0A9Q1BXW4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_22642</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BXW4"/>
    <dbReference type="OrthoDB" id="120976at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312">
        <property type="entry name" value="NACHT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312:SF2">
        <property type="entry name" value="NUCLEOTIDE-BINDING OLIGOMERIZATION DOMAIN-CONTAINING PROTEIN 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040306216" description="Ig-like domain-containing protein" evidence="2">
        <location>
            <begin position="23"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="277"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="132"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="NACHT" evidence="4">
        <location>
            <begin position="371"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8035418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="773" mass="86899" checksum="1B9F4C18A2AC0E31" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BY29</accession>
    <name>A0A9Q1BY29_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Relaxin receptor 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_21635</name>
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    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BY29"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008528">
        <property type="term" value="F:G protein-coupled peptide receptor activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009755">
        <property type="term" value="P:hormone-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24372:SF77">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24372">
        <property type="entry name" value="GLYCOPROTEIN HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="51"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="96"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="136"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="218"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="264"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="295"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="9">
        <location>
            <begin position="21"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8034689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="371" mass="41542" checksum="7FBF871EFCE46B11" modified="2023-09-13" version="1">MFPNHALRVVLWVVCLSSVVANFAVFISRLKHEIPVMRKLFSSTVSANQNTFLLNLAVADFAMGLYLLAIGLADVIFGNEYFLFALGWRKGIPCKIFGFIVFLSNVVSLLILTLVSIERFLVIVFPFGNIRLGPNLTVKLCIMSWVVGGIMALTPIILSEYVPQAFGFSDICLGLPFVPIPEMLGNEVSVISDQYGYIEDINVNNNFKGLQWVYSQIIYIYFSSACVLVITICYVCIFVSTIVAKKQSGRGYVDKAEIKLALRLSIIIWTDLICWLPVIITGILSALGTDISVDMYVWLTVLVMPVNSALNPFIYTVPTIKKKKNNEPSFIGEQRTLEKQKQDKRQKRWITTAFKAHSYESSTSPAEVSTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C1E5</accession>
    <name>A0A9Q1C1E5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cytoplasmic dynein 1 intermediate chain 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_20162</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynein intermediate chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C1E5"/>
    <dbReference type="OrthoDB" id="4189at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005868">
        <property type="term" value="C:cytoplasmic dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045504">
        <property type="term" value="F:dynein heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045503">
        <property type="term" value="F:dynein light chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010970">
        <property type="term" value="P:transport along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001896">
        <property type="entry name" value="Cytoplasmic dynein 1 intermediate chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000026">
        <property type="entry name" value="cytoplasmic dynein 1 intermediate chain 2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025956">
        <property type="entry name" value="DYNC1I1/DYNC1I2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050687">
        <property type="entry name" value="Dynein_IC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442:SF22">
        <property type="entry name" value="CYTOPLASMIC DYNEIN 1 INTERMEDIATE CHAIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442">
        <property type="entry name" value="DYNEIN INTERMEDIATE CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11540">
        <property type="entry name" value="Dynein_IC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="17"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="50"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="71"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="130"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="141"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="164"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8036246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="605" mass="67707" checksum="CCAF7DBFB5521734" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C2J1</accession>
    <name>A0A9Q1C2J1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_18938</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C2J1"/>
    <dbReference type="OrthoDB" id="10042460at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="149"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="180"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="262"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="293"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="360"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="406"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="465"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8037983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="498" mass="58408" checksum="60FF318F48999F96" modified="2023-09-13" version="1">MEMSLLAGNEEGDRRSFFPWQQSIRLSDSTKSEWPPMIWFILKITGLFYHRNVVTKRWCISCEVDNYKSSLCTDQDGHRQTDMIMWDSTDLTTCYEHKLLDDELNLLSPQHERYCKACETCWWDRYGKVSVYTEADIGAQAWNHRGSRLVSVVILLLVASLILFETIFTENKLFAKRELLLHFMSYTSFLCPLFVFPVMNICSKVRSALSGRVLGLWATSLNARYIVQRLQYLDLSKDGKERDKFICLFQINRHRHGLPSKWFLIICLVWPICCSTYRAYITFALKGYSDIAHRLNCLTVGITEFMWGCFVYMLFLMRLSFQIQLELVLDFIKKCEGNEDLCQRVVRRLTIDFRCFRDCVAVYMTMMIPLCVLGASTSITWQYLILAHIDKDCSSLWCVTERNINIMIWSDVLMFSSLGPIAIGGFNVNYLWENFQLHVKFMQNKIHQGFWNKLVRFLERTEDQVPYFSFTTILSVVSLYMAFNFDENDIDTTADIPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C3E2</accession>
    <name>A0A9Q1C3E2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Amino acid transporter AVT6E</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_18577</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C3E2"/>
    <dbReference type="OrthoDB" id="513400at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF646">
        <property type="entry name" value="SODIUM-COUPLED NEUTRAL AMINO ACID TRANSPORTER 10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="77"/>
            <end position="97"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="117"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="146"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="190"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="222"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="266"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="320"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="344"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="376"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="9">
        <location>
            <begin position="3"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="444"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="957"/>
            <end position="1052"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="450"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="512"/>
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        </location>
    </feature>
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        <location>
            <begin position="537"/>
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        </location>
    </feature>
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        <location>
            <begin position="966"/>
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        </location>
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        <location>
            <begin position="987"/>
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        </location>
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            <begin position="997"/>
            <end position="1007"/>
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        <location>
            <begin position="1033"/>
            <end position="1045"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8037690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1074" mass="119165" checksum="1B55AF5D18E3382D" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C3I0</accession>
    <name>A0A9Q1C3I0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_18592</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome 8"/>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Znf_FYVE_PHD"/>
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    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
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        <property type="entry name" value="Znf_PHD-finger"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8037702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="148" mass="16329" checksum="6CB0092AFF6C0F90" modified="2023-09-13" version="1">MAIEYCLHCSGTVKDDEKALQCDGCSRWQHLGCITGVTEEEYGGAVEEGGVIEWACFWCSSKAVEEGVLVAESTKVDAALEEYHIPQPINESSLAEEEVNPQPRTTSPVTYEVVVSGTKRGREPLVDNRGYTFNVKRRTNAVHCTSEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C410</accession>
    <name>A0A9Q1C410_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Leucine-rich repeat-containing G-protein coupled receptor 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_18982</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Eleutherozoa</taxon>
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            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
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            <taxon>Holothuriidae</taxon>
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        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8038020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome 8"/>
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        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
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        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
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        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45617">
        <property type="entry name" value="LEUCINE RICH REPEAT FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45617:SF181">
        <property type="entry name" value="LP04042P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
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        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0675">Receptor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040402903" evidence="4">
        <location>
            <begin position="22"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="341"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8038020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="384" mass="44161" checksum="39CA79CFDA125E33" modified="2023-09-13" version="1">MESCKHTQYFLVVILLQLSSTFQISVSSATKEKGNCTYNDWGKEVSCRQTGLVSIPTLSGPVKILDISDNKITQLTNQTFVMLPFLEHLDLSNNRLGEINDGTFGKYSRGTRRSLIKFLDLSENELSKIENNAFKMLSSLSELYLNDNLLTDMTKNVLGHLRSLEILWLSKNKLTHLPHDTFQGKGDLLSLFLDRNKFAILPDSIFRGCWSLEVINLSGNQLQHAPASLKDLPSLRYLYLSNNRLIDISPVTFHLWALETLTIEKNFLQCSCNLEQVQQWMFRKGVLHTNSDNICYQSDNTSVNLSTLSTFCQERSKIDDSDFRTFGGKVEYTIIVREDLIYYNSVLLTCLTLMSVIWFLSYLFSRKVTRHFRRRLKNGKVELM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C726</accession>
    <name>A0A9Q1C726_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_13676</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8039625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C726"/>
    <dbReference type="OrthoDB" id="6408700at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00024">
        <property type="entry name" value="CD_CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46585:SF1">
        <property type="entry name" value="CHROMO DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46585">
        <property type="entry name" value="INTEGRASE CORE DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="2">
        <location>
            <begin position="56"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="Chromo" evidence="1">
        <location>
            <begin position="320"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8039625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="464" mass="54708" checksum="395F3D84B2B13EDA" modified="2023-09-13" version="1">MGDTSDTFLHKIYYDLSHPASFGGINKLYDEVKRDGRYNISRNAIREWLMGQNTYTLHKPVKKNFARNRVLVNAIDHQWEMDLADLSSLSRYNKGYKFLLTCIDVLSKFAWVIPLKNKSGRTLLQAVKKILSSGRKPQQIHTDRGTEFVNKLLQKYLKDKGIHFFTTNNETKASVVERFNRTLKSKMWKYFTHRNTLKYIDILPNLVKGYNHSYHRSIKLRPVEVTKDNEDRMWRTLYPGINIKASFKFNVGDRVRISKSKLKFEKGYLPNWSEEIFIIVKRKGKPVPVYKLTDWGGEPIEGNFYESELQKVNLAQGALFRVEKVLKKRKRNGRVEYLVKWKGYPTKFNSWVTDNFYVTLPSNSSFQYFPENTLASFKTQLAEPIVLNGSWEVSLCEIQYPFNWHNVTEHNCRVQLNDREICIPPGYYDTIQDVIDAFHDLLLEEERRHVKLFSTQNHNFKDRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C7L6</accession>
    <name>A0A9Q1C7L6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">WSC domain-containing protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_14393</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WSCD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C7L6"/>
    <dbReference type="OrthoDB" id="5985073at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051589">
        <property type="entry name" value="Sialate-O-sulfotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002889">
        <property type="entry name" value="WSC_carb-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45964:SF9">
        <property type="entry name" value="SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45964">
        <property type="entry name" value="WSCD FAMILY MEMBER CG9164"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01822">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00321">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51212">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="18"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="WSC" evidence="4">
        <location>
            <begin position="84"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8040172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="445" mass="49762" checksum="8066E92CDB0A2DCB" modified="2023-09-13" version="1">MAASKEYIPCGFLCRKKGFLTILCFSVLLVALLWKNKFSLQINFPDTPCDGETCRGKKSWGIEKGSVISAVGQTFQTWSNLSAPGTYRGCINFLDSSKKDLNMVRVEESKQMTVVRCINHCSKKVNTYVGLLGGTQCYCINFTGPSDSLHYVPTSQCSAPCSGDPTYNCGGRNSTFSLYRTKIPDSRCSEISLMPEGSMPLIALASFPRSGNTWTRRLLEHSTGIYTGSIYWKSESRQSIGSKVFLGGNINYNTGSTLCIKTHLFDVHNVKEFTEGAILLLRNPYKAIVAEAFRRQMIAKDEGPERTIQFIKSGGNNWTNFVKKNAGNWLATAVNWCRNSKRLLVVNYEELQKNTSKELIRMVKFLDQPLNMTRIACAIQELPSSESTGVYLGNHGIKTRSYLTFDPFTKLLRKLLDGHIATVNATLIRYGHAPLPNYKEAKLLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C836</accession>
    <name>A0A9Q1C836_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Muscle, skeletal receptor tyrosine protein kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_13695</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8039636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C836"/>
    <dbReference type="OrthoDB" id="10055367at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098632">
        <property type="term" value="F:cell-cell adhesion mediator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070593">
        <property type="term" value="P:dendrite self-avoidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.2000.10">
        <property type="entry name" value="Frizzled cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020067">
        <property type="entry name" value="Frizzled_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036790">
        <property type="entry name" value="Frizzled_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10075">
        <property type="entry name" value="BASIGIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10075:SF100">
        <property type="entry name" value="FASCICLIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01392">
        <property type="entry name" value="Fz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50038">
        <property type="entry name" value="FZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040198222" evidence="4">
        <location>
            <begin position="23"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="91"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="FZ" evidence="5">
        <location>
            <begin position="280"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8039636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="439" mass="49095" checksum="C4B9D7680D0618F5" modified="2023-09-13" version="1">MKKIWWMLFLVLYLNILTGTHAEDNQVTTQNEPLGYKPTEHPNHVVISPQQATFPSETSPQSASLRTTLTQITSSSTTDKPATSELLLQAPVILLPLDNLTVSIGDDVLLKCAVEKNPPDPGQKITITWFKENVVIKQNTFPRITFPQARRKLKITQVQWEDRGNYFCTARNRYGVVRSTPGRLEVEKAVSIIMGIQNMTVPKDTPINSTCIVEGYPKPLIHWELNGVVYRDVSTNSPYDKQFIEFIAQNTIKLTCKAVNTITTVGETRKQSSGFIFVTNPASYCKSYNGFNCAPFGLGEVFVNASLDNPPGKLERMVSEVVDSTNAMTIPKRCKNAFNSLLCHLVFPDCEKSGPPPRPVRLCQEDCIAIKDLACLNHWKELQTIHSPLFEPYLSLGFPECTAFDSDSRDTCTRTGYFDAPKSLKTTGKVTFYVLIVVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C8G5</accession>
    <name>A0A9Q1C8G5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Leucine-rich repeat-containing protein 15</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_14251</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C8G5"/>
    <dbReference type="OrthoDB" id="1883493at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001164">
        <property type="entry name" value="GH01279p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366">
        <property type="entry name" value="IG(IMMUNOGLOBULIN) AND LRR(LEUCINE RICH REPEAT) DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366:SF170">
        <property type="entry name" value="RE50361P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040152648" evidence="5">
        <location>
            <begin position="29"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="372"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="404"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="431"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="446"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8040059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="455" mass="50072" checksum="3DD8AAA29139AEC3" modified="2023-09-13" version="1">MPTITHPISRAGCLLLLTWFVSVHPSSGRRLASSCGSICDYDEGTRKADCSNRSLNLVPQQCFSAVSLNLQGNEIDILTSGIFANFSSLKMVDISKSNMREIQTGTFSGLNSLKKLFLSGNNLTNIVDGMFEGLRNLRILNLANNLISSIGEGKFQEAGSLNKLYLADNSIHSLEADALLGLKSLNTLSLSRNLIVDIHEGTFGNTPNLENLYLGMNKITTLPKNVFQNLHSLKQLSLAANQISTVPPSVLRLESLKRLDLQANLLQDVSPLVPKLHQLTDLHLNGNPLLCSGCPEPLLEWFTMKYEDTTMECSMPDGTIVKVSSLVASDPEVVFVPIVSTPYQPHTAVNGKTDCSDDKPMQDSDGDVIRTVLFTVVPLLFMILIGSIILLEFQARQRIARKENPRIFSPDGTSKYRQHEYRDSDDDENIQGLSNSGAFNSVPSVREPKRMESSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C8G7</accession>
    <name>A0A9Q1C8G7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">C-type lectin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_14967</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C8G7"/>
    <dbReference type="OrthoDB" id="441660at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050111">
        <property type="entry name" value="C-type_lectin/snaclec_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22803">
        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C-type lectin" evidence="1">
        <location>
            <begin position="22"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8040622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="155" mass="18201" checksum="D06FE8DD7EB0EFC6" modified="2023-09-13" version="1">MSTRMDRIRGLLLSRYFSKITSWVEAQDFCRNVGANGYSTGNNYQRSDLTSIHSAAEENFLYNYWVSCRDSRETDVRTLFIGLNDPLRNRVWKWADGSEVDYLNWAPGEPNNLNNYGEYYGTMYNSKKWNDEHVGDVKEFTFICKMPRYSCGTYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C945</accession>
    <name>A0A9Q1C945_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Alpha-N-acetylgalactosamine-specific lectin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_14685</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C945"/>
    <dbReference type="OrthoDB" id="418245at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050111">
        <property type="entry name" value="C-type_lectin/snaclec_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22803">
        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040398389" evidence="2">
        <location>
            <begin position="19"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="3">
        <location>
            <begin position="27"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8040404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="162" mass="18314" checksum="790B98B271F7FE9B" modified="2023-09-13" version="1">MKFVIFVTTAAFFATTAAVCPTEWTPYDGSCYRFVNQYRQRWAEADAECQSAAPDKDSHLVVIDSEMENQWVHDWWVSMLSPLSGTGFEYIWLGYTDASSEGDFVWVTGSTSSYINWGGRQPDKGTEANCATMVTKYANDPGKWDDGKCVAKRAYVCEYRDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C9Z4</accession>
    <name>A0A9Q1C9Z4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 12">Tyrosine aminotransferase</fullName>
            <shortName evidence="12">TAT</shortName>
            <ecNumber evidence="5 12">2.6.1.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HOLleu_11836</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Nanhai2018</strain>
            <tissue evidence="15">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Transaminase involved in tyrosine breakdown. Converts tyrosine to p-hydroxyphenylpyruvate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>L-tyrosine + 2-oxoglutarate = 3-(4-hydroxyphenyl)pyruvate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:15093"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:36242"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="EC" id="2.6.1.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12 13">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 2/6.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 12">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.5" evidence="5 12"/>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C9Z4"/>
    <dbReference type="OrthoDB" id="7042322at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004838">
        <property type="term" value="F:L-tyrosine-2-oxoglutarate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006559">
        <property type="term" value="P:L-phenylalanine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006572">
        <property type="term" value="P:L-tyrosine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000048">
        <property type="entry name" value="tyrosine aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005958">
        <property type="entry name" value="TyrNic_aminoTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005957">
        <property type="entry name" value="Tyrosine_aminoTrfase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01264">
        <property type="entry name" value="tyr_amTase_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01265">
        <property type="entry name" value="tyr_nico_aTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45744">
        <property type="entry name" value="TYROSINE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45744:SF2">
        <property type="entry name" value="TYROSINE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000517">
        <property type="entry name" value="Tyr_transaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00753">
        <property type="entry name" value="ACCSYNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 15" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="10 12" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0828">Tyrosine catabolism</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="14">
        <location>
            <begin position="43"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="13">
        <location>
            <position position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00015959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR000517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000517-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ8041110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="417" mass="46415" checksum="88D5D9C8CEC770B6" modified="2023-09-13" version="1">METNGQPTKRSKWTIASSLMAKRTFNPIRTVVDGMKLEPNPDKDLISLSIGDPTVFGNLGPSQEMKDAVVSALNSGKSNGYAPSVGYEHARAAVAKRFSTPDIPLKSKDVVFACGCSEALEMAILVLANPGQNVLIPKPGFSLYWTICQAYGIDSKHYNLLPERSWEIDLDHLESLIDENTSCIVVNNPSNPCGSVFTKDHILDICSVARRHKLPIIADEVYADMVFSESEFHSVSSLSEEIPVLTCGGLAKRFLCPGWRMGWIIMQSRTGAFDEEIRHGLFNLSTRVLGPCTLIQAALPDIFDNSSPEFYSHAMEVFESNAKLAYKTLKQIKGLNPIMPQGAMYLMCGLDPSYFADITDDQDFVEKLMCEESVFCLPAKVFEYPNYFRIVLSVPPEKVKTACQRIEEFCLRHSKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CAP6</accession>
    <name>A0A9Q1CAP6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_12715</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CAP6"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8041807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="86" mass="9092" checksum="4453AB83899BFE94" modified="2023-09-13" version="1">MSCKNLSVTLLSIVRNIKVHVNLDLSTSMCESTEVGKGEGVEGDEKPLRPNGSTEASAEIRGCITPLTLGCAPLAVHLKTLKLTLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CD58</accession>
    <name>A0A9Q1CD58_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_09674</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8042817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CD58"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8042817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="82" mass="9959" checksum="F274C5B2B90E812A" modified="2023-09-13" version="1">MNGKKSFYNRVWRFIHVLCVVTNGKRLIYHVMQFACLHDHQTHINTSCNMKERETLQLWLEWLPFSPNIKKIMKTFKSNDYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CDL9</accession>
    <name>A0A9Q1CDL9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nose resistant-to-fluoxetine protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_09375</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8042589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CDL9"/>
    <dbReference type="OrthoDB" id="6434228at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006621">
        <property type="entry name" value="Nose-resist-to-fluoxetine_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052728">
        <property type="entry name" value="O2_lipid_transport_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11161">
        <property type="entry name" value="O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11161:SF0">
        <property type="entry name" value="O-ACYLTRANSFERASE LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20146">
        <property type="entry name" value="NRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00703">
        <property type="entry name" value="NRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040388818" description="Nose resistant-to-fluoxetine protein N-terminal domain-containing protein" evidence="1">
        <location>
            <begin position="25"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="Nose resistant-to-fluoxetine protein N-terminal" evidence="2">
        <location>
            <begin position="52"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8042589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="254" mass="28351" checksum="985722C073069CA5" modified="2023-09-13" version="1">MHSTQCRIVLVISVMVLFPHSAVSQLSPEMIQGVVWPRQRIPVTDIQSHEVSSHCKRNLSDLPKAFDAFGKPAAGILVGNINWMGHFDECESLDGFQYCVVEFNISVAQSNPVMPFMLYGVCAPENCSESDVYNSMDFFVGEFSGNTFVCFFFQDPQYILIRLCSGEGGSVCVAWMSTFVPVLNMSVMHLKDLIWTCFLFCALIQIAVLEYSNKEPLSYIYTSGISGFNEKRNLCIHDGVCVCVFVYEGVCACV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CFL8</accession>
    <name>A0A9Q1CFL8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_11401</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8044050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CFL8"/>
    <dbReference type="OrthoDB" id="10059408at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47481">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47481:SF31">
        <property type="entry name" value="OS01G0873500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8044050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="102" mass="11510" checksum="DA58AB96ECD03864" modified="2023-09-13" version="1">MRDAKDNGREALKIPREHYLSKGKPKIFVLYTELTSLKKSAQESITNYLIRAETAATSLKTSSETISDSLLIAMILKGLPPKFKPFSTVITQKDKTSKFKEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CHM7</accession>
    <name>A0A9Q1CHM7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Phosphofurin acidic cluster sorting protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_08064</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PACS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CHM7"/>
    <dbReference type="OrthoDB" id="28829at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019381">
        <property type="entry name" value="PACS1/2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057541">
        <property type="entry name" value="PACS1/2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13280:SF17">
        <property type="entry name" value="KRUEPPEL TARGET AT 95D, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13280">
        <property type="entry name" value="PHOSPHOFURIN ACIDIC CLUSTER SORTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25332">
        <property type="entry name" value="C2_PACS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10254">
        <property type="entry name" value="Pacs-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphofurin acidic cluster sorting protein 1/2 N-terminal C2" evidence="5">
        <location>
            <begin position="25"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphofurin acidic cluster sorting protein 1/2 C-terminal" evidence="4">
        <location>
            <begin position="465"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="183"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="294"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="686"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="191"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="343"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="399"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="423"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="686"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="715"/>
            <end position="730"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF10254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF25332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8045126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="879" mass="97446" checksum="F44FE918FABCA62E" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CIE7</accession>
    <name>A0A9Q1CIE7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_08122</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CIE7"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8045174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="86" mass="9925" checksum="F10505204F19E681" modified="2023-09-13" version="1">METEQSDFSLNNITSDGLLCQRRFDVTPTCKRKHSGHWTDVADPNSNSIRRQNAYRRESSLTGDSVGRVAKRDDRTTYCERVVLQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CLA5</accession>
    <name>A0A9Q1CLA5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_05372</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CLA5"/>
    <dbReference type="OrthoDB" id="10052666at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="136"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8046629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="286" mass="31243" checksum="4B256912FCDB6E72" modified="2023-09-13" version="1">MAGQSDLIKKAAEGALEEIKKCAPEEYTRLAAGTDIKDKILKAAEATAKETLLLAEEFAGQPNAIAERIAKHLPDDRVKLIQGGLNIPTFRIEIVKKDDGKHWLEFTRDGKEFLPADELVSRFDVDWGITLQYTSILIEAILLVMSAVGIPISLSAYVIKKTVNEAAASIRSKASSKLRLALDDFVGAWDAAASNPSKKAQALFDVIKESYSATGIIWNIIQSLCSNMEWYEWMETSAKVTAMIIAAINTDGAPLIAEISLIILSAEDFAKKIANVTQLCDIKKAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CLD8</accession>
    <name>A0A9Q1CLD8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_06780</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CLD8"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040254733" evidence="1">
        <location>
            <begin position="19"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8047717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="97" mass="10748" checksum="21066EE559D94FD6" modified="2023-09-13" version="1">MRTVGFFLFGLTTLPVAADNTASVHVWINIVLVFCVVPQYQVECASNAMHLKLIKDLFKDQAENAYLRPVNDTNEAVSVGFRFTPTISVLVSCRNLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CMU2</accession>
    <name>A0A9Q1CMU2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Protein piccolo</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_06604</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Synapse</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10059918at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048788">
        <property type="term" value="C:cytoskeleton of presynaptic active zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042734">
        <property type="term" value="C:presynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044325">
        <property type="term" value="F:transmembrane transporter binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048791">
        <property type="term" value="P:calcium ion-regulated exocytosis of neurotransmitter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050806">
        <property type="term" value="P:positive regulation of synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048167">
        <property type="term" value="P:regulation of synaptic plasticity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000300">
        <property type="term" value="P:regulation of synaptic vesicle exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041282">
        <property type="entry name" value="FYVE_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010911">
        <property type="entry name" value="Rab_BD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039032">
        <property type="entry name" value="Rim-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12157">
        <property type="entry name" value="REGULATING SYNAPTIC MEMBRANE EXOCYTOSIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12157:SF25">
        <property type="entry name" value="REGULATING SYNAPTIC MEMBRANE EXOCYTOSIS PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02318">
        <property type="entry name" value="FYVE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50916">
        <property type="entry name" value="RABBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0770">Synapse</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RabBD" evidence="12">
        <location>
            <begin position="508"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="domain" description="FYVE-type" evidence="11">
        <location>
            <begin position="565"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="10">
        <location>
            <begin position="2252"/>
            <end position="2347"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="9">
        <location>
            <begin position="2411"/>
            <end position="2538"/>
        </location>
    </feature>
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</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
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        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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</entry>
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                <person name="Chen T."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <text>ATP + H2O = ADP + phosphate + H(+)</text>
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    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF20209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8048291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1500" mass="172703" checksum="D67BFBB7AC0A1686" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CP80</accession>
    <name>A0A9Q1CP80_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Heparan sulfate glucosamine 3-O-sulfotransferase 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_00610</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CP80"/>
    <dbReference type="OrthoDB" id="411451at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008467">
        <property type="term" value="F:[heparan sulfate]-glucosamine 3-sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037359">
        <property type="entry name" value="NST/OST"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10605:SF72">
        <property type="entry name" value="HEPARAN SULFATE 3-O SULFOTRANSFERASE-B, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10605">
        <property type="entry name" value="HEPARAN SULFATE SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="17"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="Sulfotransferase" evidence="7">
        <location>
            <begin position="161"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="active site" description="For sulfotransferase activity" evidence="3">
        <location>
            <position position="170"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="255"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="263"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="375"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR637359-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR637359-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR637359-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8048338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="430" mass="50198" checksum="3627DB7A4A734299" modified="2023-09-13" version="1">MCYLSEGMAVNCTKRDIWIFLLVCVLFLSYHAYYNGRCQSPCEKRRQPLPSTYSRLVGITEASVLNTSEWYVACLGDRKESDSLQVGLNDVAILKEFPPLDKIDILVKQAFLRLSPEKANAFCANALLILKEESYWSLTVTERWKFALKIFATVGIRQRLPDVIGIGVKKSGSSALRLYLAHHPNIRLPDIYSEAHYFDWKYFMSNKESYMAMMPLLAEDEICYEKTPRYFVTSTAPTNIARDVSPRTKFVICIRDPVKRALSDWHHEQTLLIQRKNFSCVGCTALSEGIRFTEKILTKNGEVDSQSEFIDTSNYARHFEEWLQVFPFSQFFILKEEDIRNNPLPTLQKLEKFLGVPRYYKQEMFVYNSTMNATCFYEDDHELISCLRLQNALPHPEPNETVIKKMRDFFRPHNKRFEELTGMKFEWTDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CQ86</accession>
    <name>A0A9Q1CQ86_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_01285</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CQ86"/>
    <dbReference type="OrthoDB" id="10063844at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="322"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8048815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="775" mass="89129" checksum="6141ADD1BE10823D" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CR23</accession>
    <name>A0A9Q1CR23_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_02680</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CR23"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8049781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="150" mass="17267" checksum="DEF01F348CADA112" modified="2023-09-13" version="1">MLPLSRSVFVIRPKLPWYNEDLQICKRRKRKAETVWLSSGTTEDRATYISLRNMYKNMLNDAQNNYLNDCIAKAGNDPKKLFRSIAAMTGKRDELVFPDATSNTQLSEDFKDFFWSKVDKLRNELDNMGIAPFDCGTSDHSATLDCSKRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CRJ3</accession>
    <name>A0A9Q1CRJ3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_02277</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CRJ3"/>
    <dbReference type="OrthoDB" id="3263820at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46579">
        <property type="entry name" value="F5/8 TYPE C DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46579:SF1">
        <property type="entry name" value="F5_8 TYPE C DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8049505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="547" mass="62156" checksum="64FA04B9FBDA1739" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CSU6</accession>
    <name>A0A9Q1CSU6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein aurora borealis</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_04071</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BORA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CSU6"/>
    <dbReference type="OrthoDB" id="10020858at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007088">
        <property type="term" value="P:regulation of mitotic nuclear division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060236">
        <property type="term" value="P:regulation of mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023252">
        <property type="entry name" value="Aurora_borealis_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14728">
        <property type="entry name" value="PROTEIN AURORA BOREALIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14728:SF2">
        <property type="entry name" value="PROTEIN AURORA BOREALIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15280">
        <property type="entry name" value="BORA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02038">
        <property type="entry name" value="AURORABORA"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0498">Mitosis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="130"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="215"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="255"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="304"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="502"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="215"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="265"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="309"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="347"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="388"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="505"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="524"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="548"/>
            <end position="559"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8050756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="559" mass="60891" checksum="ABE386F03F11A8BF" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CT89</accession>
    <name>A0A9Q1CT89_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_03265</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CT89"/>
    <dbReference type="OrthoDB" id="413361at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00024">
        <property type="entry name" value="CD_CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46585:SF1">
        <property type="entry name" value="CHROMO DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46585">
        <property type="entry name" value="INTEGRASE CORE DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="2">
        <location>
            <begin position="52"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="Chromo" evidence="1">
        <location>
            <begin position="318"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8050169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="593" mass="69767" checksum="324A2E211685080F" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CTZ8</accession>
    <name>A0A9Q1CTZ8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_03935</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CTZ8"/>
    <dbReference type="OrthoDB" id="9995178at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="1">
        <location>
            <begin position="43"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8050649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="220" mass="25250" checksum="DE54D392B4013910" modified="2023-09-13" version="1">MRYRNVDIPLQCSVTFYRQICSDMRDFMKHLKEHIVVGTAISCPFNNCYASFSNKSTFSSHLSRNHKPDATDQISAHLVQLKVPVYKDNLENNNGVIVDDEVVDELELLHGTDNAQDFYFQNLMFYLKLESKYLLPASTIQHIIEQFKDIQSWSDQDFKKLFASLTDDLEIPEQRAKQLVEEVGASDLLVASSDGPLRSDYIRKPITNPSSIMLPQLKLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1GXH9</accession>
    <name>A0A9Q1GXH9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Neurofilament heavy polypeptide</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_30929</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1GXH9"/>
    <dbReference type="OrthoDB" id="5418055at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040189757" evidence="2">
        <location>
            <begin position="23"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="163"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8028632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="212" mass="22027" checksum="26B42DF4F0032961" modified="2023-09-13" version="1">MLALRVAVVVVLVALFIDAAPAGRGGRPGGRPGSRPGGPPGGRPGGRPGGRPVRPPPGGRPGGKPDEGDRPDEGDRPDEGDRPDEGDMPSEGERPDEGDMPSEGDRPDEGDRPDEGDMPSDGDRPDEGDRPDEGDMPSEGDRPDEGDRPDEGDMPSEGDMPSEGDRPSEGDRPDEGDRPSEGDIPSDGDRPPEGDSPSEGEMPSEGDRPPLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H2P9</accession>
    <name>A0A9Q1H2P9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_29546</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H2P9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8029986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="133" mass="15257" checksum="789D952BBF229F1B" modified="2023-09-13" version="1">MGFPEVKKQHFSRIPKIIIGQKAKFPKNSQDRSYGVTRGQKAKYPKCSQDHHLGSPEVKKQISQRNTTGVMSIHVLIFLFFIHAIFPSICPGRPIMTELAKFVGVFHYSTFIEVILPVQIQHTILSSFVHYYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H7K5</accession>
    <name>A0A9Q1H7K5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_22623</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H7K5"/>
    <dbReference type="OrthoDB" id="5843397at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040347888" description="Ig-like domain-containing protein" evidence="2">
        <location>
            <begin position="21"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="256"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="54"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8035400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="323" mass="36073" checksum="3606CD77B0228708" modified="2023-09-13" version="1">MIFSLTEIIAISFPVALVQGLSEDIRCDMSSGKLLDGINGFVECNGFLFQHVCLICSISSRATDVRWYGNNRQLTQDEYNLQEDHAGGIEVNRCDSERYSLRLKSPSLENDNTSYSCVNHGVHVSQFVLKLKAETKLFINSTRIFQGKLLGNESNASSIARCVIEGAVPPFSLTWSLNGVVQDMKTFNYSAEVYSFDLLYDSSHFRSQNVATCSSQGKYIRNDKSLSFHLFKIHAIDDGDDPFQTDSTNSFHHVEWILIPSAGVILAVIVLCLILKGVHNPSLRLDFVRNRFSGTQVEDVGNVANSYYYVEHFANGSYAESGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H8K3</accession>
    <name>A0A9Q1H8K3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Guanine nucleotide-binding protein subunit alpha-13</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HOLleu_17791</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Nanhai2018</strain>
            <tissue evidence="16">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the G-alpha family. G(12) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H8K3"/>
    <dbReference type="OrthoDB" id="5817230at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031526">
        <property type="term" value="C:brush border membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005834">
        <property type="term" value="C:heterotrimeric G-protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031752">
        <property type="term" value="F:D5 dopamine receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031683">
        <property type="term" value="F:G-protein beta/gamma-subunit complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007188">
        <property type="term" value="P:adenylate cyclase-modulating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007266">
        <property type="term" value="P:Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00066">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000692">
        <property type="entry name" value="Guanine nucleotide-binding protein subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.400.10:FF:000004">
        <property type="entry name" value="Guanine nucleotide-binding protein subunit alpha-12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000754">
        <property type="entry name" value="Guanine nucleotide-binding protein subunit alpha-13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.400.10">
        <property type="entry name" value="GI Alpha 1, domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000469">
        <property type="entry name" value="Gprotein_alpha_12/13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001019">
        <property type="entry name" value="Gprotein_alpha_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011025">
        <property type="entry name" value="GproteinA_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218">
        <property type="entry name" value="GTP-BINDING PROTEIN ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218:SF360">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN SUBUNIT ALPHA HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00503">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00318">
        <property type="entry name" value="GPROTEINA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00440">
        <property type="entry name" value="GPROTEINA12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00275">
        <property type="entry name" value="G_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47895">
        <property type="entry name" value="Transducin (alpha subunit), insertion domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51882">
        <property type="entry name" value="G_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 14" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0564">Palmitate</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0807">Transducer</keyword>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="48"/>
            <end position="53"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="156"/>
            <end position="157"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="182"/>
            <end position="188"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="207"/>
            <end position="211"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="276"/>
            <end position="279"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="333"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ8037064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="361" mass="42123" checksum="329BE3F16FF87BF2" modified="2023-09-13" version="1">MAGSVFGCCLSAEEKDQRSKSKAIDKDIQKHKQQLRREVKVLLLGAGESGKSTFLKQMKIIHGQKFDDTDLKEFRTIIYGNIIKGMKVLADARSKLNIPWGDENCERYANTVLGYDSSRALEPAVFQQYVQPCKILWQDSGIQSAFDRKREFQLADSVRYFLDEIDRIGKRQDYIPTESDILHSRKATKGISENVIEIKNVPFRFVDVGGQRSQRQKWFQCFENVTSILFLVSSSEFDQVLMEDRVTNRLFESCNIFDTIVNHRYFVEVSIILFLNKSDLLEEKVKRVSIKDYFPAYNLDPHDLSKVQSFILNMFDTRRRERSKALFHHFTTAVDTDNIKYVFTFVRDTILSENLRRLMLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H9W7</accession>
    <name>A0A9Q1H9W7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">NLR family CARD domain-containing protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_12131</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H9W7"/>
    <dbReference type="OrthoDB" id="427518at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312">
        <property type="entry name" value="NACHT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312:SF2">
        <property type="entry name" value="NUCLEOTIDE-BINDING OLIGOMERIZATION DOMAIN-CONTAINING PROTEIN 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040346557" evidence="2">
        <location>
            <begin position="19"/>
            <end position="1029"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="471"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="349"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="NACHT" evidence="4">
        <location>
            <begin position="600"/>
            <end position="723"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8041337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1029" mass="118309" checksum="DDC76DF3CA8825B1" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HB55</accession>
    <name>A0A9Q1HB55_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Abasic site processing protein HMCES</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Embryonic stem cell-specific 5-hydroxymethylcytosine-binding protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Peptidase HMCES</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">SRAP domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_16421</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SOS response-associated peptidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HB55"/>
    <dbReference type="OrthoDB" id="2111841at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106300">
        <property type="term" value="P:protein-DNA covalent cross-linking repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1680.10">
        <property type="entry name" value="SOS response associated peptidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003738">
        <property type="entry name" value="SRAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036590">
        <property type="entry name" value="SRAP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13604:SF0">
        <property type="entry name" value="ABASIC SITE PROCESSING PROTEIN HMCES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13604">
        <property type="entry name" value="DC12-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02586">
        <property type="entry name" value="SRAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143081">
        <property type="entry name" value="BB1717-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0190">Covalent protein-DNA linkage</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="219"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="221"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="244"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="259"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="275"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8038866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="302" mass="34567" checksum="FE63802A244B0FD8" modified="2023-09-13" version="1">MLMKWGLVPSWHKGDPAAMAYKMNNCRSEGMLEKASFKRPFEKGQRCVILADGYYEWHTDSNKKKQPYFIYFPQDKPLTDLPLPESYEFDFDSANMEETEDKTEEWTGHRLLTMAGIFDKWNPPDGGEPLYTYSIITIEAAPSVSWIHHRMPVMLDGDKSVRDWLDFGTVPTSEALTLVKALDCLTCHPVTPQMGNIRYKEPDCIKQFDLEAKKEEKPKNTFMSSWLKKSTKQDPQSKTASSQDVKEGVKKEPAEKKTGLTSWLQKANKPSTLKRAMDSASPEKKLPSKVIKLEEEANSEKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HBK3</accession>
    <name>A0A9Q1HBK3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_10240</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HBK3"/>
    <dbReference type="OrthoDB" id="6783748at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00303">
        <property type="entry name" value="retropepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000020">
        <property type="entry name" value="Transposon Tf2-6 polyprotein-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041577">
        <property type="entry name" value="RT_RNaseH_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17919">
        <property type="entry name" value="RT_RNaseH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="3" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="11">
        <location>
            <begin position="117"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase" evidence="12">
        <location>
            <begin position="400"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="48"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="52"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="95"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8043242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="701" mass="78469" checksum="B5F798A4C7C23AE4" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HCF7</accession>
    <name>A0A9Q1HCF7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_14557</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein-tyrosine phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="3"/>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HCF7"/>
    <dbReference type="OrthoDB" id="6058203at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00047">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000102">
        <property type="entry name" value="Receptor-type tyrosine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050348">
        <property type="entry name" value="Protein-Tyr_Phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134:SF562">
        <property type="entry name" value="PROTEIN-TYROSINE-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134">
        <property type="entry name" value="RECEPTOR-TYPE TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="14" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="468"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="13">
        <location>
            <begin position="360"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="11">
        <location>
            <begin position="710"/>
            <end position="964"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="12">
        <location>
            <begin position="908"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="11">
        <location>
            <begin position="989"/>
            <end position="1242"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="12">
        <location>
            <begin position="1163"/>
            <end position="1233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="611"/>
            <end position="662"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ8040303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1253" mass="138965" checksum="57E310569B8653A1" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HFN3</accession>
    <name>A0A9Q1HFN3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_00163</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HFN3"/>
    <dbReference type="OrthoDB" id="10062908at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009689">
        <property type="entry name" value="DUF1280"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31424">
        <property type="entry name" value="PROTEIN CBG23806"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31424:SF3">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06918">
        <property type="entry name" value="DUF1280"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8048012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="297" mass="34135" checksum="F08C2BF081C93CA3" modified="2023-09-13" version="1">MPLRQLCENAPSFLKKQGIFLSRGSSDEQLNHELSQASLQKDLHSLNKSRTMMPASEFLGLKADMCSSWKRNRELKRWLKRYGAHTECEQKLRQQQAQIIRDNLLGECLPFLFEEDDGTVIKSTPCVRVKDLKTKIIQQLEEYERLNLLTWHSVIPENEIWIKIGGDKGGATFKQMFQVANVESPNAPLNTTVFCIFPAKDSFYNLKLAFQSLQAQVNTLQESTWRGKKFRIFGFGDYEYLCKMYGLGGASGRHCCLWCVETKSNMKLAPHPDKMPEARTLATLRRDLERYKDGAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HFU1</accession>
    <name>A0A9Q1HFU1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNase H type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_10590</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HFU1"/>
    <dbReference type="OrthoDB" id="7765170at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09276">
        <property type="entry name" value="Rnase_HI_RT_non_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002156">
        <property type="entry name" value="RNaseH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31025">
        <property type="entry name" value="SI:CH211-196P9.1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31025:SF25">
        <property type="entry name" value="ZINC FINGER (C2H2)-60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00075">
        <property type="entry name" value="RNase_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50879">
        <property type="entry name" value="RNASE_H_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase H type-1" evidence="1">
        <location>
            <begin position="240"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8043486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="577" mass="65508" checksum="1369D0693CE9D060" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HI41</accession>
    <name>A0A9Q1HI41_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Solute carrier organic anion transporter family member</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_08822</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the organo anion transporter (TC 2.A.60) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HI41"/>
    <dbReference type="OrthoDB" id="5062115at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015347">
        <property type="term" value="F:sodium-independent organic anion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:monoatomic ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043252">
        <property type="term" value="P:sodium-independent organic anion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17336">
        <property type="entry name" value="MFS_SLCO_OATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036058">
        <property type="entry name" value="Kazal_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004156">
        <property type="entry name" value="OATP"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00805">
        <property type="entry name" value="oat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11388">
        <property type="entry name" value="ORGANIC ANION TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11388:SF142">
        <property type="entry name" value="SOLUTE CARRIER ORGANIC ANION TRANSPORTER FAMILY MEMBER 5A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03137">
        <property type="entry name" value="OATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100895">
        <property type="entry name" value="Kazal-type serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00282">
        <property type="entry name" value="KAZAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51465">
        <property type="entry name" value="KAZAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="33"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="69"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="99"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="158"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="190"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="243"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="326"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="359"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="396"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="515"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="550"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="607"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="domain" description="Kazal-like" evidence="10">
        <location>
            <begin position="438"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="275"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="280"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8045751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="661" mass="72423" checksum="C513B975A52C8BAE" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HLI3</accession>
    <name>A0A9Q1HLI3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Direct IAP-binding protein with low pI</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_02617</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the Smac/DIABLO protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HLI3"/>
    <dbReference type="OrthoDB" id="6153032at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008631">
        <property type="term" value="P:intrinsic apoptotic signaling pathway in response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051402">
        <property type="term" value="P:neuron apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009062">
        <property type="entry name" value="Smac/DIABLO-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015142">
        <property type="entry name" value="Smac_DIABLO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32247">
        <property type="entry name" value="DIABLO HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32247:SF3">
        <property type="entry name" value="DIABLO IAP-BINDING MITOCHONDRIAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09057">
        <property type="entry name" value="Smac_DIABLO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46984">
        <property type="entry name" value="Smac/diablo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00033049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00046319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8049738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="251" mass="27963" checksum="BF04255B26E5E78A" modified="2023-09-13" version="1">MVTPLTFRWRTMLTCYVKKTMTVIRSFRNVAGYSETCSHLLTQDTFPWAFSSRSKIFIVSGGCLLTTLCAVPFVSPSAPGRELRKLEDVDSSGLSHRSLLRNASAAVVTSAIALVSQSLASLNELTSQYVKEVYNLVKLYDDHNAIAGKQLGKMEDDLWQEIIQTRVNLTEKKRQIQEVEAVLTSSLLVLQSAGEAAFNAGVENSAIEAKIKQQQTTEYLSMIQNDMRKAETELTKIQAKNILETTDDEET</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HM24</accession>
    <name>A0A9Q1HM24_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA helicase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_03944</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HM24"/>
    <dbReference type="OrthoDB" id="10063152at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051055">
        <property type="entry name" value="PIF1_helicase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47642">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47642:SF5">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="417"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="417"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="428"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8050656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="553" mass="63436" checksum="AEDDE22F65C3E9D6" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0Y8W0</accession>
    <name>A0A9Q0Y8W0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Solute carrier family 22 member 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_44420</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000866">
        <property type="protein sequence ID" value="KAJ8017902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0Y8W0"/>
    <dbReference type="OrthoDB" id="2261376at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24064">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="32"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="68"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="1"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8017902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="187" mass="21716" checksum="84E0B58DFD7AC401" modified="2023-09-13" version="1">MFGRRNTLLFCYTTGGLFGIVTVFSSNVWMYLILRFCIATVLKGIGLCCFVLDFLLAATELVAPSKRIFVGNFMWLFFAAGYTVLAGSAKLIPDWRILQLVLTLPYIPLIIITYFFVPESPRWLINQKRSEDAEKVLRKMAKVNKRIISDDFMERAFNRNEVCKYKRFEPLIKTLVRKRRPLIRGPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0Y9L8</accession>
    <name>A0A9Q0Y9L8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_43333</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000272">
        <property type="protein sequence ID" value="KAJ8018593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0Y9L8"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8018593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="124" mass="14696" checksum="57078CF7F0BBFA84" modified="2023-09-13" version="1">MNISFEISRVIIFKGHDMPYLSWRTVSLKNISFEISRVIIFKGHDMPYLSWRTVSLKNISFEISRVIIFKGHDMPYLSWRTVSLKNISFEISRVIIFKVYKPVCLANKSTLCWLFNTLMIRKIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YB26</accession>
    <name>A0A9Q0YB26_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_41024</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8019433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YB26"/>
    <dbReference type="OrthoDB" id="5957937at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8019433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="123" mass="14353" checksum="7A2D3D1EA507932E" modified="2023-09-13" version="1">MAEDERVSNGNSENENVPNSKESQGQGDSNLRNFISEEFHNQRLNGPPDGYQISDQRAVQFLQRALSESQRMANETTRSQRQQQRLYAELGEDIFQQWTREHLEETVKNAMRKAEEDAMHVER</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YBP0</accession>
    <name>A0A9Q0YBP0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Helitron helicase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_43353</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000280">
        <property type="protein sequence ID" value="KAJ8018581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YBP0"/>
    <dbReference type="OrthoDB" id="416437at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8018581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="51" mass="6090" checksum="F76BA84D075C7268" modified="2023-09-13" version="1">MIRQLGIPTWFMSFSAAETRWFHLLRILGKTVHSKVYSDAQLLNMTWHEKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YBV8</accession>
    <name>A0A9Q0YBV8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Reverse transcriptase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_42130</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000034">
        <property type="protein sequence ID" value="KAJ8019330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10025340at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00303">
        <property type="entry name" value="retropepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09274">
        <property type="entry name" value="RNase_HI_RT_Ty3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.370:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 17.6-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000032">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 297-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon gypsy-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000020">
        <property type="entry name" value="Transposon Tf2-6 polyprotein-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041373">
        <property type="entry name" value="RT_RNaseH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17917">
        <property type="entry name" value="RT_RNaseH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50804">
        <property type="entry name" value="SCAN_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SCAN box" evidence="9">
        <location>
            <begin position="162"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase catalytic" evidence="11">
        <location>
            <begin position="676"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase" evidence="10">
        <location>
            <begin position="1092"/>
            <end position="1269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="249"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="45"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="253"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="277"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8019330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1485" mass="168887" checksum="B4BCF86C0946E1DF" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YCE6</accession>
    <name>A0A9Q0YCE6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Enoyl-CoA hydratase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_39781</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YCE6"/>
    <dbReference type="OrthoDB" id="448450at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43802">
        <property type="entry name" value="ENOYL-COA HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43802:SF1">
        <property type="entry name" value="IP11341P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8020243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="68" mass="7885" checksum="EAA84C1751D2FFD4" modified="2023-09-13" version="1">MLRERTLVKTERRGPVFLICINRPERRNAVSIETGIELKKAIHSFQDDENARVAVLYGTGQIWSYLPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YD60</accession>
    <name>A0A9Q0YD60_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Membrane-bound O-acyltransferase domain-containing protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_40090</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YD60"/>
    <dbReference type="OrthoDB" id="286734at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030258">
        <property type="term" value="P:lipid modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049941">
        <property type="entry name" value="LPLAT_7/PORCN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004299">
        <property type="entry name" value="MBOAT_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13906:SF4">
        <property type="entry name" value="LYSOPHOSPHOLIPID ACYLTRANSFERASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13906">
        <property type="entry name" value="PORCUPINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03062">
        <property type="entry name" value="MBOAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="91"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="220"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="362"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="403"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="438"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="466"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="472"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="483"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8020488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="493" mass="57018" checksum="844DF0FDC8D5F842" modified="2023-09-13" version="1">MEIFLVRAISDATKLKYTEVVFVTCQFTALFFSFFFRRYLKPTNTSTTTRYTAQLLLGISMVLFSFGRGTIHFLIDSIVCYVMTLTLDPRYSQLFVTFFSVSYMSAIHLYFQFSNLGVNNLDYIGPLMILTEKLSSLAWSVHDGFARKEEDLNDLQQHMVVREYPSPLKFFSFIFCPQNLLVGPVTYYADYCAFIEGKPFMLKIKDEKGEEKLVYTEPSALYAITGKLFFTLLSSLCLLTLVPRFPIMGNVDDDFIQNYSFLFRLGWLIISIEVAKSKYFLAWVWADAINNAAGLGFNGYDDKGNPKWDGVTNVRIWEFQTATSLKVLIDNWNISGARWLRHVCFDRMPYQKKLTTFMLSSIWHGFYPGYFFTFGSASIMVAASQKVRVNLRHHFTSSSRLKAGYDVFTWACTHLCLAYFIAPFTFLKFDLCVKYFNSFYWFLHVLAFMAIIFLPGRKRSKTPAKTEMNGVYESNHNSTDGKVNNHTETKKEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YDR3</accession>
    <name>A0A9Q0YDR3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_41270</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8019616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YDR3"/>
    <dbReference type="OrthoDB" id="10037294at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040196068" evidence="1">
        <location>
            <begin position="20"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8019616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="77" mass="8343" checksum="DFCCE3647D3B26EF" modified="2023-09-13" version="1">MKIDAFFVVLLFWQATVTTFGSDTCPPGVDRVNCFVSPCLFATCPAYPGATCVANYCGGCFAEWYQSGVLVNCLIQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YE18</accession>
    <name>A0A9Q0YE18_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Tripartite motif-containing protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_40849</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8021082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YE18"/>
    <dbReference type="OrthoDB" id="19092at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047153">
        <property type="entry name" value="TRIM45/56/19-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25462">
        <property type="entry name" value="BONUS, ISOFORM C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25462:SF296">
        <property type="entry name" value="MEIOTIC P26, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="16"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8021082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="114" mass="13478" checksum="7D4BE3FC6364704B" modified="2023-09-13" version="1">MAKYIITRLREDFVHCTICTEPHNEPKMLPCLHSFCLPCLERWARNNHSQSFSCPKCRRKVNLPRDGVCSLPHNFFLVSLMERLEEINRLSNEHQDYNCNICRNGNDTMFCLEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YF53</accession>
    <name>A0A9Q0YF53_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_38956</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YF53"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8021682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="73" mass="8321" checksum="4B52FBC4A9FA178A" modified="2023-09-13" version="1">MEKVLPLLEENGGKARLIASYTNKCRQLICQPMVYARKSTKRVAISTPFLHLCFIAFTQNDVSVMSMHKVYSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YFZ6</accession>
    <name>A0A9Q0YFZ6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_39289</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YFZ6"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8021943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="114" mass="12672" checksum="3AD3FB3E339B7608" modified="2023-09-13" version="1">MGVFTEGHDDSEVRIVSAATVSVRTAAIFRKELQYGNIKEHFRTDSQVVLGYKQMQQNGSTPLWGTESSKSLMGANRNNGTMFPASTTQLNLLHWDLNAENLKKCGMALCFSEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YGY7</accession>
    <name>A0A9Q0YGY7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_38401</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YGY7"/>
    <dbReference type="OrthoDB" id="8041494at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004748">
        <property type="term" value="F:ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009263">
        <property type="term" value="P:deoxyribonucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000358">
        <property type="entry name" value="RNR_small_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005331">
        <property type="entry name" value="Sulfotransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23409:SF21">
        <property type="entry name" value="CAPSID PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23409">
        <property type="entry name" value="RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE SMALL CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03567">
        <property type="entry name" value="Sulfotransfer_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8021255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="611" mass="69101" checksum="43036105115F6A90" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YHF6</accession>
    <name>A0A9Q0YHF6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_39576</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8022164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YHF6"/>
    <dbReference type="OrthoDB" id="6159398at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040158123" description="Ig-like domain-containing protein" evidence="2">
        <location>
            <begin position="26"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="336"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="48"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8022164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="448" mass="51169" checksum="3D07D763A615295A" modified="2023-09-13" version="1">MDLSNTLVSVICIFIVSHTVRNVSAIRWEFKDVFEPGNVLYVKETMNVELCCSKVAKSTVSFISWSPEGTLSDKSITLFNASRPTTRSNKYYFSELSDTFTLYDMGKQDGGNYYCSQILQPYGRPRLLYVILKVVKIPKEKPICSTNYRYPVIFHDEMKDNFEFNCSTETGNPPLKIDIFVDDAIFSPDTSWTKNGVTTLSTITYMTEGLNNSTYSCSVRQELPFPYNERYEETCSFGPMHILPGFNITITPTSAFFKEGDNVTFTCMSNVSGVEKKWQNITRGKYTITNNGERSYLNVLDLKPPLSGRIALECQGSYGSRLVTAIAHVNTKRTRLFLLIFGLVIFVVGFLDYRRRKSNRRRQRRIKIAKDEYERRTASEISKSKAINEDSVSHQAYDRSIVTQDPSHKGKRYYDKVSKSYIYDSEGYLSPSKERCTVDVDVDDGVNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YIE8</accession>
    <name>A0A9Q0YIE8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Isovaleryl-CoA dehydrogenase, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_38192</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8023108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YIE8"/>
    <dbReference type="OrthoDB" id="9988775at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008470">
        <property type="term" value="F:3-methylbutanoyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006552">
        <property type="term" value="P:L-leucine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.110.10:FF:000001">
        <property type="entry name" value="Acyl-CoA dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884:SF12">
        <property type="entry name" value="ISOVALERYL-COA DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00072">
        <property type="entry name" value="ACYL_COA_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="7">
        <location>
            <begin position="3"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="6">
        <location>
            <begin position="81"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8023108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="194" mass="21087" checksum="CA19D6F68192D9E9" modified="2023-09-13" version="1">MLKMGELGLHGITVPEKYGGVGGGYLDHLIICEEIARASPSVCMVYGAHSNLCLNQISRNGNEEQKERLMPKLISGEHLGALAMSESSSGSDVISMRCKAEKKGDYYIMNGTKSWITNGPDADVLVVYAKTNPNAEAKHAITTFIIEEGMEGFSRGKKLDKLGMRGSNTGDLIFEDCKVPGIYSFMVNVKNTVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YIF1</accession>
    <name>A0A9Q0YIF1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">CUB and zona pellucida-like domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_37700</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YIF1"/>
    <dbReference type="OrthoDB" id="10063988at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.4100">
        <property type="entry name" value="Zona pellucida, ZP-C domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055355">
        <property type="entry name" value="ZP-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042235">
        <property type="entry name" value="ZP-C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001507">
        <property type="entry name" value="ZP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14002:SF43">
        <property type="entry name" value="DELTA-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14002">
        <property type="entry name" value="ENDOGLIN/TGF-BETA RECEPTOR TYPE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00100">
        <property type="entry name" value="Zona_pellucida"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51034">
        <property type="entry name" value="ZP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="141"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="ZP" evidence="4">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8022719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="188" mass="20544" checksum="75C685771FAA2CD2" modified="2023-09-13" version="1">MGFNSLQRGKFPDRLQMTTKSVLVVTPPLLGCGINDAPVRVLTPYKQTQADFEITSFRFRKDLSHPESQIWIHCEVVVCDLSDANSECRQGCEQSRHRRSAKSPPLKTKRMVQGPIVLSSKHNDNSDIAAMSQNKDGSSGVLTAFLSAVSLIMMIGLVAMAVVLTKVISSSKQQGYQPLPNPSGENVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YP42</accession>
    <name>A0A9Q0YP42_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Zinc finger CCHC domain-containing protein 24</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_37614</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YP42"/>
    <dbReference type="OrthoDB" id="10038672at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027377">
        <property type="entry name" value="ZAR1/RTP1-5-like_Znf-3CxxC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033446">
        <property type="entry name" value="ZCCHC24_Znf-3CxxC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025829">
        <property type="entry name" value="Zn_knuckle_CX2CX3GHX4C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13696">
        <property type="entry name" value="zf-CCHC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17180">
        <property type="entry name" value="Zn_ribbon_3CxxC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01328">
        <property type="entry name" value="zf-3CxxC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="6">
        <location>
            <begin position="181"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="51"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8022651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="280" mass="31050" checksum="7FB2B7D818F5220B" modified="2023-09-13" version="1">MKNLMMADLQTQLGPIDYNFNNNWSSLSRQATNQLLNNSLVYLQPLDEPLQGSSNRNSTRSSPGQMTGSRLTSRDIAALLNAPNIGLNSSNEHSGGLSSLGLSGGWSEKYFAGDDIKSFTSYSDASSTVETEQSSMLGCQYTDTASEVSEVSHVIDQFGDLSLLPVSERKPGKRPPPTYLCHLCFNKGHYIKDCPKARPKGEGLTPYQGKKRCFGEYKCPKCKRKWMSGNSWANMGQECIKCHINVYPHKQRPLEKPDAVDVTDQTLDHSHLWKSTSLGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YPJ4</accession>
    <name>A0A9Q0YPJ4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_33932</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8026168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YPJ4"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8026168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="96" mass="11188" checksum="D67B0518C85049A0" modified="2023-09-13" version="1">MTIREHRYGNKIGYISDTPVSFFSKKCEKKVNLQTTDTAMGLNPLARLINHREEIDQDVQVLVKATGMRGQFYYPLAFRFESVSDRWVIRTEQNTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YPQ3</accession>
    <name>A0A9Q0YPQ3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_34051</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8026257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YPQ3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8026257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="274" mass="31051" checksum="D6FAB8AB936490FC" modified="2023-09-13" version="1">MVSVRKIRSFCIRTALLCCLVYFSETSSNLMIWNPGSNDLSLPPRSPSLEKETLVSSPYTKFLEKFFSEVGLVRSKKSKSEFSVHTQQNLIQGKDGQKRSDTEEDTCYMLPAAYSKIQRVIGMVTDLRGRIERDTRSSSLSRRLVPNCYNESLTTIGGMISSRMTQYLSMFPWFEIIYPSYSDVISLAWTANSTFATIKTRLAEMQQNCPCDFSNIANSTFITTSCMLPTPVPGEPNMNALVITSLNELDFILRSIDHHTSRMDYSSQRKVPLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YPV8</accession>
    <name>A0A9Q0YPV8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Homogentisate 1,2-dioxygenase</fullName>
            <ecNumber evidence="4">1.13.11.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Homogentisate oxygenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Homogentisic acid oxidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Homogentisicase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">HOLleu_31214</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">Nanhai2018</strain>
            <tissue evidence="19">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 16">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 4/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the homogentisate dioxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.5" evidence="4"/>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YPV8"/>
    <dbReference type="OrthoDB" id="1689029at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004411">
        <property type="term" value="F:homogentisate 1,2-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006559">
        <property type="term" value="P:L-phenylalanine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006572">
        <property type="term" value="P:L-tyrosine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07000">
        <property type="entry name" value="cupin_HGO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000034">
        <property type="entry name" value="Homogentisate 1,2-dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046451">
        <property type="entry name" value="HgmA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046452">
        <property type="entry name" value="HgmA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005708">
        <property type="entry name" value="Homogentis_dOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01015">
        <property type="entry name" value="hmgA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11056">
        <property type="entry name" value="HOMOGENTISATE 1,2-DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11056:SF0">
        <property type="entry name" value="HOMOGENTISATE 1,2-DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04209">
        <property type="entry name" value="HgmA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20510">
        <property type="entry name" value="HgmA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="10 16" id="KW-0408">Iron</keyword>
    <keyword evidence="6 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-0585">Phenylalanine catabolism</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0828">Tyrosine catabolism</keyword>
    <feature type="domain" description="Homogentisate 1,2-dioxygenase N-terminal" evidence="18">
        <location>
            <begin position="13"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="Homogentisate 1,2-dioxygenase C-terminal" evidence="17">
        <location>
            <begin position="265"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="15">
        <location>
            <position position="276"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="319"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="325"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="334"/>
        </location>
        <ligand>
            <name>homogentisate</name>
            <dbReference type="ChEBI" id="CHEBI:16169"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00030437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00033225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR605708-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR605708-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF04209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF20510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAJ8026408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="380" mass="43118" checksum="234020922593C02A" modified="2023-09-13" version="1">MNLPLKTPDVQEPSLKDSPQKCPYGLYAEQLSGTAFTAPRVANKRSWLYRIRPSVLHEPFKPYPHKTLVDDWTQWHPNPNQLRWHPFDLPANDVKSDFVDGMATVCGGGDVRSRHGIAVHIYACNKSMENRCLYNSDGDFLIVPQKGTLCITTEFGKMKVDPNEIVVIQQGMRFSVQISEPSRGYILEVWDGHFTLPDLGPIGANGLANPRDFLTPVACYEDIQAPYTIISKFQGKLFFAQQDHSPFDVVAWHGNYAPYKYDLEKFMVINTVSFDHADPSIFTVLTCQSTRPGVAIADFVIFPPRWSVGENTFRPPYYHRNCMSEFMGLITGHYEAKAFMFESSIGLAVTKWADSACQKVDEKYFECWQPLAKHFKGPAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YR97</accession>
    <name>A0A9Q0YR97_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Mu-type opioid receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_34901</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YR97"/>
    <dbReference type="OrthoDB" id="9946013at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004983">
        <property type="term" value="F:neuropeptide Y receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00637">
        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000611">
        <property type="entry name" value="NPY_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24228">
        <property type="entry name" value="B2 BRADYKININ RECEPTOR/ANGIOTENSIN II RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24228:SF72">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01012">
        <property type="entry name" value="NRPEPTIDEYR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="25"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="62"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="98"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="140"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="193"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="245"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="272"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="42"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8024867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="319" mass="36187" checksum="95DF98F90F843D46" modified="2023-09-13" version="1">METIHVSDVYTTSQTYAGLDDPNEVLIFAIIDVIISLLGIPGNFLVIFAVSSSRKLQTSTNFFVANLAFSDLVTCLTMPFHAFTILNGRPPSDIVCKLVGGVFSISVGVSIFNLTLIAFNRAYLITKPRSQYDQLYSRKNIALMLICNWVYVTLAVCVPPAFNIGALGYTRRYRVCITDISHTMNFYYDLLKALLTAPFILILILCYVCIYFEVRKTTNASLHICNKNDQLQQQMRKRQIAVTKNLFIIVCIYISCVAPFFIVSVQPSIYHVIPWVLELALFNSCLNPFIYGFNHPQFKEVFSQALFCRFKKSIHLRGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YS15</accession>
    <name>A0A9Q0YS15_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Autophagy-related protein 9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_34344</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Phospholipid scramblase involved in autophagy. Cycles between the preautophagosomal structure/phagophore assembly site (PAS) and the cytoplasmic vesicle pool and supplies membrane for the growing autophagosome. Lipid scramblase activity plays a key role in preautophagosomal structure/phagophore assembly by distributing the phospholipids that arrive through ATG2 from the cytoplasmic to the luminal leaflet of the bilayer, thereby driving autophagosomal membrane expansion.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Preautophagosomal structure membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the ATG9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YS15"/>
    <dbReference type="OrthoDB" id="2020634at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034497">
        <property type="term" value="P:protein localization to phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007241">
        <property type="entry name" value="Autophagy-rel_prot_9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13038">
        <property type="entry name" value="APG9 AUTOPHAGY 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13038:SF10">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04109">
        <property type="entry name" value="ATG9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0072">Autophagy</keyword>
    <keyword evidence="8 10" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="58"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="114"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="274"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="357"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="391"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="614"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="734"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="614"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="635"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="762"/>
            <end position="771"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU364027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8024436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="771" mass="88170" checksum="C4BDF8B1757DA559" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1B949</accession>
    <name>A0A9Q1B949_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transposase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_43128</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000213">
        <property type="protein sequence ID" value="KAJ8018726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1B949"/>
    <dbReference type="OrthoDB" id="6616463at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31751:SF42">
        <property type="entry name" value="PROTEIN CBG10204"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31751">
        <property type="entry name" value="SI:CH211-108C17.2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="97"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8018726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="429" mass="49201" checksum="326DC1C42737C16C" modified="2023-09-13" version="1">MCDAQTQTEKQCNDKECQTTGPDVVVNSVAVGRDHTYAKKPSQTSDVVSIVPPELIPETQPQQHQSKSPPPPPIRNSDNATGEIDDVQKDPTYSPDKEDDQDDNDELPEERPFWNKTVEDCKYLVFSSCLNKLFYKIKCDECYCNVESIKRSVVGTGLFVTLQCISGHERDYLIPEVNSTWLKEQPSLISSLQGKPISIVGDGRCDSPGYSAKYCSYTLMDIESEKSIDFELVQVSETGSSVKMEAVGFKRCFSRFLDDHNLNVDKFASDRHVSFRKIMKDNYKGVKHNFDVFHLAKNISSKLRRIAKKKDNEDIECWIKSINNHVWWCSRNCEKDPSMLVEMWMSLTHHTAGKHSFHDDNRFTLFKECRHPAIDPSTNRRKKWLVEGSSAHQALVKVVLNKRLLNDMKHLAEFMHTRSFSCRRSEICT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1B9R9</accession>
    <name>A0A9Q1B9R9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_43312</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000267">
        <property type="protein sequence ID" value="KAJ8018605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1B9R9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="354"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="357"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="393"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="418"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8018605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="468" mass="52754" checksum="A6A694334603B8A7" modified="2023-09-13" version="1">MEDVFRECNLIWRFSGNIDDIARHGLMIDAGVSGVGYENNLIDTHTLHSILLYADRLDPNRPPEEEIDLAPLAKMIYGLSFTKAMVRDYINGEQNIPYVDTVRAITKHMLPQGLLMVKGDDGLDVVANTKMFHAKMLTKGYVNDLNRVTMQIPLDHTIDHMLLKSESGDLNLYYVIGPHNELLVGPGAGNENLRAASPARVIKGRRVQNMVKLTGDPTTGKRNTQSDPTTIVYDQQLTGAFVFTKSTQNIEYVLSHMAARCLKYSACKHLADKSCRTIGAIFLATIFKDDKRHVVTTSKAGNESLMLSNFYFCKGSQWKYRDFFYEKNDFLGTDTKEFGERVSVVDTNYQADPDSLLTLSNKPVPTSTPQLPKKNDRENSDRIILINETPKTTIREKGKDRKQENDEDRFSLFAPSTSREKDNSRGKEAKKRGKDSNAGTTIPKRSAKVQRTTLLEEDDSDRLFLPLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BA69</accession>
    <name>A0A9Q1BA69_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Endonuclease-reverse transcriptase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_42357</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000059">
        <property type="protein sequence ID" value="KAJ8019205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BA69"/>
    <dbReference type="OrthoDB" id="425681at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF8">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8019205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="142" mass="17040" checksum="BF66BA3901B3CA9E" modified="2023-09-13" version="1">MLGISTLMYASETWTLSRDAKNKIDAFEMWIYRKMLKVSNKDRMSNEEVLNRLKAKKELLSELKNIKLQYMGYILRSSGLQKQLLEGKVGSRRLRGKQRNTWWADIRKWTGKSLYDLARTAENRTKWRTWHPEPLRDKEPYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BBL0</accession>
    <name>A0A9Q1BBL0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">60S ribosomal protein L11</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_40310</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the universal ribosomal protein uL5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BBL0"/>
    <dbReference type="OrthoDB" id="1734943at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1440.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002132">
        <property type="entry name" value="Ribosomal_uL5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031309">
        <property type="entry name" value="Ribosomal_uL5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020929">
        <property type="entry name" value="Ribosomal_uL5_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022803">
        <property type="entry name" value="Ribosomal_uL5_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057266">
        <property type="entry name" value="Ribosomal_uL5_euk/arc-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031310">
        <property type="entry name" value="Ribosomal_uL5_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003258">
        <property type="entry name" value="PRK04219.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11994">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00281">
        <property type="entry name" value="Ribosomal_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00673">
        <property type="entry name" value="Ribosomal_L5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002161">
        <property type="entry name" value="Ribosomal_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55282">
        <property type="entry name" value="RL5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00358">
        <property type="entry name" value="RIBOSOMAL_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6 9" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL5 N-terminal" evidence="7">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL5 C-terminal" evidence="8">
        <location>
            <begin position="56"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8020660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="167" mass="19169" checksum="C33A0A331F1B3CD3" modified="2023-09-13" version="1">MKDLRIRKLCLNICVGESGDRLTRASKVLEQLTGQTPVFSKARYTVRSFGIRRNEKIAVHCTVRGAKAEEILERGLKVREYELRKGNFSDTGNFGFGIQEHIDLGIKYDPGIGIYGMDFYVVLGRPGYSVSHKRRKKGRIGFRHRVTKEESMKWFQQKYDGILLPGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BG61</accession>
    <name>A0A9Q1BG61_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_36621</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8024019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BG61"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8024019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="92" mass="10642" checksum="4B0A401496537473" modified="2023-09-13" version="1">MCASSREDGYIRELPFRQVAALCALCVHHQEKMVTSWTCHLVKWQLMCHMCESSRDDGYIRELSYGRVAALCVLCINHQKKMVPIESCHLDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BIH4</accession>
    <name>A0A9Q1BIH4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_31823</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BIH4"/>
    <dbReference type="OrthoDB" id="775972at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000003">
        <property type="entry name" value="Protein CBG25708"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000063">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 297-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF11">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="2">
        <location>
            <begin position="123"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="296"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="399"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8026867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="432" mass="50285" checksum="8C7E9A20EF211A2E" modified="2023-09-13" version="1">MTMEEIDKCSQNDPEIQELRNRIDTGKWVGCNKSYYAVRHELTTFGNMVLRGTRIVMPRPLREQTLKLAHEGHQGIVKTKQRLRQKVWWPNIDREVEGMIRQCHACQVVNKAAQAEPIQPSKLPEGPWEDLAVDLCGPFPSGEYIIVLTDYYSRWPEATVLCSITSQTIINWLNHVFSSHGYPKSITCDNGAQFVSKEFCTYLSDHGIHHRRVTPYWPQANGQVERMNKVFLKLIRASVVEGKDWRKELTNFLLIYRSSPHITTGLSPAEMLFKRQIRTKLPQVDKMSVSQKQLKEAMRHDRAQKQSYKNYADRQRRTQVRDIKPGDKVLVKCEKKNKLSPAFYPIPFKVVKKMGNCLELMRPDGIKFRRNVSHTKLYKGGEGEIGDANLYDLEEDDICDNGEGENIPNHQRENDRPRRNRHPPRWLDDYIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BIN2</accession>
    <name>A0A9Q1BIN2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Beta-chimaerin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Beta-chimerin</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Rho GTPase-activating protein 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HOLleu_32418</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Nanhai2018</strain>
            <tissue evidence="16">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BIN2"/>
    <dbReference type="OrthoDB" id="3196451at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20806">
        <property type="entry name" value="C1_CHN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.555.10:FF:000005">
        <property type="entry name" value="Chimaerin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000019">
        <property type="entry name" value="Chimaerin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000025">
        <property type="entry name" value="Chimaerin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020454">
        <property type="entry name" value="DAG/PE-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051854">
        <property type="entry name" value="Rho-type_GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46075">
        <property type="entry name" value="CHIMERIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46075:SF2">
        <property type="entry name" value="RHO GTPASE ACTIVATING PROTEIN AT 5A, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00008">
        <property type="entry name" value="DAGPEDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SH2" evidence="13">
        <location>
            <begin position="259"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="14">
        <location>
            <begin position="402"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="15">
        <location>
            <begin position="465"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="159"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="159"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00073081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00076015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00077047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ8027308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="664" mass="75934" checksum="A5BF1738FBF9D86E" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BIS8</accession>
    <name>A0A9Q1BIS8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Hydroxyacid oxidase 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_32476</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BIS8"/>
    <dbReference type="OrthoDB" id="25826at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003973">
        <property type="term" value="F:(S)-2-hydroxy-acid oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001561">
        <property type="term" value="P:fatty acid alpha-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02809">
        <property type="entry name" value="alpha_hydroxyacid_oxid_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012133">
        <property type="entry name" value="Alpha-hydoxy_acid_DH_FMN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000262">
        <property type="entry name" value="FMN-dep_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037396">
        <property type="entry name" value="FMN_HAD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578:SF149">
        <property type="entry name" value="2-HYDROXYACID OXIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578">
        <property type="entry name" value="S -2-HYDROXY-ACID OXIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01070">
        <property type="entry name" value="FMN_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51349">
        <property type="entry name" value="FMN_HYDROXY_ACID_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FMN hydroxy acid dehydrogenase" evidence="4">
        <location>
            <begin position="1"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8027350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="210" mass="22805" checksum="C8EF18CEC4D803FA" modified="2023-09-13" version="1">MPSVTKRNKFVFTKSFLRRTGCIANLQGAKDDIAKAKSNGDTQLFHYMSDQAAWDQSWDDIAWIRSITRLPVILKGIITDENAKEAVAAGVQGIIVSAHGGRQLDGVKAPIEALPEVVDAVRDSGIEIYMDGGVRSGRDIFKALALGAKAVFIGRPAAWGLAHNGTDGVIEVIDMMKEEFIDTMSLCGCQSLGELNRSFVECKTQLCCKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BJD3</accession>
    <name>A0A9Q1BJD3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Retrovirus-related Pol poly from transposon opus</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_29737</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8027708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BJD3"/>
    <dbReference type="OrthoDB" id="6113439at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="CDD" id="cd00303">
        <property type="entry name" value="retropepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="495"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="551"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="495"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="552"/>
            <end position="565"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8027708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="645" mass="71997" checksum="A5C1DA99CEB8AA97" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BJM9</accession>
    <name>A0A9Q1BJM9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Zinc finger protein ZFAT</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_29954</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8027877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="3535323at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24379:SF121">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24379">
        <property type="entry name" value="KRAB AND ZINC FINGER DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="954"/>
            <end position="982"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="1035"/>
            <end position="1063"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="1064"/>
            <end position="1091"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="1092"/>
            <end position="1120"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="1201"/>
            <end position="1228"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="1268"/>
            <end position="1296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="83"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="324"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="384"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="487"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="91"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="113"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="135"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="192"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="253"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="278"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="342"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="494"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="514"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8027877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1527" mass="170311" checksum="400813B96F77EFDA" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BMM8</accession>
    <name>A0A9Q1BMM8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_28721</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BMM8"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8029349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="52" mass="5696" checksum="5A92EDA73911EA56" modified="2023-09-13" version="1">MQDPVVSIIGHTSVNLGTYPVGKTHLLPCVLAGCQKYLLLREGGLYDTVMHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BNY2</accession>
    <name>A0A9Q1BNY2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methyltransferase FkbM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_26486</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BNY2"/>
    <dbReference type="OrthoDB" id="9998228at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006342">
        <property type="entry name" value="FkbM_mtfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052514">
        <property type="entry name" value="SAM-dependent_MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01444">
        <property type="entry name" value="fkbM_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34203">
        <property type="entry name" value="METHYLTRANSFERASE, FKBM FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34203:SF15">
        <property type="entry name" value="SLL1173 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05050">
        <property type="entry name" value="Methyltransf_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase FkbM" evidence="1">
        <location>
            <begin position="36"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8030157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="245" mass="26953" checksum="ABE02901332B26B8" modified="2023-09-13" version="1">MTISHAIITRGGWNEDVISMFCHWMSTAPEEAYFVDVGAYVGTVAIGVAACGYSVFAFEPHAANFNFLQESIALNPSYSSQIWPYQKALGNTTGTTCVSASSSDQSNARIVPGRGTSVDYQCPQGIVPITTLDEVTKNFQIWAINIDAQGFDPAVIAGAWKHLSGKTPPRYIRFKVETGMYNWQTLNTELNLEDILRGMQDLGYSLRDEDNDIIVNVKQHLLNVGHFATYMTAVYHGRPEGRSGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BPW0</accession>
    <name>A0A9Q1BPW0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">pseudouridine 5'-phosphatase</fullName>
            <ecNumber evidence="7">3.1.3.96</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Pseudouridine-5'-monophosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_26874</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>psi-UMP + H2O = pseudouridine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10944"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17802"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58380"/>
            <dbReference type="EC" id="3.1.3.96"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.96" evidence="7"/>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BPW0"/>
    <dbReference type="OrthoDB" id="40579at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990738">
        <property type="term" value="F:pseudouridine 5'-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07529">
        <property type="entry name" value="HAD_AtGPP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.240:FF:000001">
        <property type="entry name" value="Haloacid dehalogenase-like hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000055">
        <property type="entry name" value="Haloacid dehalogenase-like hydrolase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="entry name" value="Putative phosphatase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045228">
        <property type="entry name" value="Gpp1/Gpp2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18901">
        <property type="entry name" value="2-DEOXYGLUCOSE-6-PHOSPHATE PHOSPHATASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18901:SF38">
        <property type="entry name" value="PSEUDOURIDINE-5'-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01135">
        <property type="entry name" value="C1.5.6:_HAD__Beta-PGM__Phospha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00052504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00066578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00083904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8030441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="231" mass="26039" checksum="3B42028C08E57152" modified="2023-09-13" version="1">MGVNVRANISHIIFDMDGLLLNTETIYEEVYRSLVEKYGKPYPFELKVKCMGRKASEAAQVIIDDLDLPISVQEWLDVSQTAVRAKFPEAKLLPGVEKLVRHLHKHNIPIAVATGSSSTNYKVKTTHHKELFSLFHHILCSGDDPEVKRGKPNPDIFEIAARRFNGETPSPDKVLVFEDAFLGVEAAKAAKMHCIMIPEKRLDRKYTTSASLVLDSIEELNLEEWGLPPFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BQI7</accession>
    <name>A0A9Q1BQI7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">NLR family CARD domain-containing protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_27491</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BQI7"/>
    <dbReference type="OrthoDB" id="427518at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312">
        <property type="entry name" value="NACHT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312:SF2">
        <property type="entry name" value="NUCLEOTIDE-BINDING OLIGOMERIZATION DOMAIN-CONTAINING PROTEIN 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040308452" evidence="3">
        <location>
            <begin position="19"/>
            <end position="1060"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="272"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="NACHT" evidence="4">
        <location>
            <begin position="443"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="789"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="801"/>
            <end position="811"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8030940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1060" mass="121717" checksum="1AACB83D9249DAE0" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BRH5</accession>
    <name>A0A9Q1BRH5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_24623</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BRH5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="93"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8031435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="150" mass="18046" checksum="DBAA3469C0F41DCE" modified="2023-09-13" version="1">MNDLQNSFIHSFIHSFIHSFIHTFIHSFIHSFNQSFIHSVIHSFNHPFALSFFLPFSLPSFLFSFFLSFFLSFFLSFFLACFLSFFLSFFRPFFLSSFFLFFLSFLLPYFPSFFLSFSLSLFVSFFLSCLLSCFLASLNLQRLFYFRRKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BRR0</accession>
    <name>A0A9Q1BRR0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_24785</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BRR0"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="289"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8031555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="377" mass="42210" checksum="67B35E69D25CA6FA" modified="2023-09-13" version="1">MIEFVSDKRAKMWTKLLLIIVCSWNVARYAVGRCPEFHKPVLKIGGVFGKTVIFPCNLGKKCYEGMWQNNNLGKRMHALDCNEDHCVATNDSESKNTLYLYNLTYDQVSSYDCVCKEPQRILYVCYDLTAVCQLEMTVGNMKTIYNGSYLSNVQRTYLVDINEGNSFNIKCQRGANRASNCTDHGSVASMPLNGCAYTCSLPVAPNVDPCIVQVILQVNDTGINFTFWHVTDKPITSTSKPTRWPPSLIRAITPDHPFTSEVSTSLPESDKTRLIDPWLPPDTALHTTVIYILIYCCSVGVMLLLIATSMVRIYHIYYKGEGKYKADVYRPGSIYGQPAPNKDYEKYADVVSDKNDYKTSSCLLSKDCALASSGDAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BS27</accession>
    <name>A0A9Q1BS27_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF4806 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_25141</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BS27"/>
    <dbReference type="OrthoDB" id="8859298at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032071">
        <property type="entry name" value="DUF4806"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34153:SF2">
        <property type="entry name" value="SI:CH211-262H13.3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34153">
        <property type="entry name" value="SI:CH211-262H13.3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16064">
        <property type="entry name" value="DUF4806"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4806" evidence="1">
        <location>
            <begin position="136"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8031817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="219" mass="25258" checksum="EAC8B1213AB9173E" modified="2023-09-13" version="1">MIRRPSSPLEQVVRRLSEHHPEIPCLLKKNSLRKPHFDGPIPRNFVVTIQYKERQTGNYVVIISSGNNCIQVGSNIAIVHNFCTVGEIITEKSMITLLIEVKAEMKEIKPHVKHLLNIQQSTTTEDEEEDIAFEELSLPCTDVDELFQLNEKLKINKTVHKELLSILSTTGGRNLREVIYRMMRELMLNCVLRHFNWTGQGSKHSFRDLALGHVMERHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BSB3</accession>
    <name>A0A9Q1BSB3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Reverse transcriptase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_24956</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10000497at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09274">
        <property type="entry name" value="RNase_HI_RT_Ty3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000115">
        <property type="entry name" value="Polyprotein of retroviral origin, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.370:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 17.6-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000032">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 297-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon gypsy-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
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        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="10">
        <location>
            <begin position="848"/>
            <end position="1006"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase" evidence="9">
        <location>
            <begin position="1262"/>
            <end position="1439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="314"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="581"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="681"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="78"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="314"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="329"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="356"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="581"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="625"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="650"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="692"/>
            <end position="721"/>
        </location>
    </feature>
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            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
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        <source>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8031686.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1654" mass="188679" checksum="550CB31FECE273E3" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BSC6</accession>
    <name>A0A9Q1BSC6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_25066</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:G protein-coupled peptide receptor activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:hormone-mediated signaling pathway"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR24372:SF77">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24372">
        <property type="entry name" value="GLYCOPROTEIN HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
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        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
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    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="34"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="74"/>
            <end position="96"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
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            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="199"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="235"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="8">
        <location>
            <begin position="1"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8031760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="272" mass="30260" checksum="B0A43BF69ABD7789" modified="2023-09-13" version="1">MGVYLSAIGIADAAFGKDYFLSAFGWRSGNTCKGIGFIGFSANVASILILTFVSVERLCTIVFPFGKDLFRSKLTVIICVAIWVASCAMALTPIILSEFITELFGFSDVCLGLPINSVPEISSFQVTEIRLRIWGEDHATRKDSDINSLQWVYSQIVYIYFSATCVSIITFCYISIFISAIRSKKQSGRPGSNKDEIKMALRIFIIVGTDLLCWLPLIITGILSNLGYEISTDVYVWFAIFVLPINSAFNPLIYTLPAIKRRKENEPSFIAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BTF4</accession>
    <name>A0A9Q1BTF4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">E3 ubiquitin-protein ligase TRIM56</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_25829</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BTF4"/>
    <dbReference type="OrthoDB" id="261644at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16584">
        <property type="entry name" value="RING-HC_TRIM56_C-V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047153">
        <property type="entry name" value="TRIM45/56/19-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25462">
        <property type="entry name" value="BONUS, ISOFORM C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25462:SF296">
        <property type="entry name" value="MEIOTIC P26, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101898">
        <property type="entry name" value="NHL repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZF_BBOX"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="17"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="7">
        <location>
            <begin position="93"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="250"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8032330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="727" mass="83518" checksum="5237B4818C66BE1D" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BTM8</accession>
    <name>A0A9Q1BTM8_HOLLE</name>
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        <submittedName>
            <fullName evidence="4">D-galactoside-specific lectin</fullName>
        </submittedName>
    </protein>
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        <name evidence="4" type="ORF">HOLleu_26109</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
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            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
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        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="6120134at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
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        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22827">
        <property type="entry name" value="Gal_Rha_Lectin_SUL-I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000922">
        <property type="entry name" value="Lectin_gal-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043159">
        <property type="entry name" value="Lectin_gal-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46780">
        <property type="entry name" value="PROTEIN EVA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02140">
        <property type="entry name" value="SUEL_Lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SUEL_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040149608" evidence="2">
        <location>
            <begin position="27"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="SUEL-type lectin" evidence="3">
        <location>
            <begin position="79"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8032557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="172" mass="18558" checksum="6398D15EE96CBD60" modified="2023-09-13" version="1">MFGFRAPKFLALFFISIIVFNKNVDSEREPRPFHCTATGPTDTTVQPSSALTTNQDTTVQPSSALTSSQDTVAAKFMVVCEGLTMNITCSGKENIVISDAVYGRLVKGSVACPDPLEGRDITINTNCQSDQTSKVREMCDGKSMCSVGVSNGEFGDPCSGTYKYLNVTYMCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BU71</accession>
    <name>A0A9Q1BU71_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_26349</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
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    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="8959203at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
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        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="44"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8032724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="216" mass="25570" checksum="BA07622DC73930D7" modified="2023-09-13" version="1">MRKDEIKRYVLEYLVDESILPSTVLETAITVPTDNTFELKRLEMEMNKIRLKEMERQMQKEKEEREMQMQRKGKRGRKCKCKCRGKKGAREHEFRLKQLEFGVIKGSDPKIGLDTGGFDVSKHVKFVPKFQEDNVEKFFNHFEKLGEQLKWPRDKWSILIQSNFTGKAQEVYSALSIEDSMDYDKVKKAKLQAYELVPELIAKNLESIVRRIHKHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BUB0</accession>
    <name>A0A9Q1BUB0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Centrosome-associated FAM110 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_22834</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8032784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BUB0"/>
    <dbReference type="OrthoDB" id="10028183at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025741">
        <property type="entry name" value="FAM110_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14160">
        <property type="entry name" value="FAM110_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Centrosome-associated FAM110 C-terminal" evidence="2">
        <location>
            <begin position="927"/>
            <end position="1037"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="128"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="230"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="488"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="520"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="559"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="708"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="811"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="142"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="168"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="230"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="276"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="309"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="319"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="530"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="573"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="837"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="850"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="919"/>
            <end position="931"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8032784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1079" mass="120088" checksum="FDE61386922620BA" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BUU8</accession>
    <name>A0A9Q1BUU8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mediator of DNA damage checkpoint protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HOLleu_23195</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Nanhai2018</strain>
            <tissue evidence="16">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="342264at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17744">
        <property type="entry name" value="BRCT_MDC1_rpt1"/>
        <property type="match status" value="1"/>
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</entry>
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    <sequence length="150" mass="17539" checksum="F2B18568B6852ED2" modified="2023-09-13" version="1">MLLPHRHRHRHRHRHRHHHHHQRENYPMGENGVDVIAFIFILYTGLRAAIDKLHENVLQFKVHITKKSTGCWFTLHAAEHSYGLDLTQVNRQSRPVFLITITCNSSIPGTLWQAQYQLRVFPHQLSEEVTLQAIRWAVSSDSLNSGMAQM</sequence>
</entry>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
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    <sequence length="381" mass="44211" checksum="2FF26C532C9E21CA" modified="2023-09-13" version="1">MVCLWNTFEVFEDTIITIIIINIIINIVVVIIIISITIIFIIDIIITATCPFGYSAIFNNSCYRISNDTDPTLSRSDARKLCESESAHLVFIESKEEDAFLINMTSPDTQYWIGANGLPNNFYWDDGSKIEFKPIGEQNIAFDDLRGCYRMWIAGKESTWPDAPCFRKYNYICEYNLERRTKQCPDDISICTELDWVALNWDWEHTAPELKNSVPAYFDGEVFSDFMLNYASRGIHHLVYRSNDSSNNWCSFRLVTDCMTWTELPVLNTSYALVTNWLRWHEASEACTFYDSNLVTVENEDENTRLQVFISSIGINVWIGLSKEDGWKWQRRNSTSYFFNWGDGEPKSWNSCTVLDPVTAKWKTKPCDSAVPFVCERRLEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BYK5</accession>
    <name>A0A9Q1BYK5_HOLLE</name>
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        <submittedName>
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        <name evidence="2" type="ORF">HOLleu_21957</name>
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        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
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            <taxon>Holothuriidae</taxon>
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        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BYK5"/>
    <dbReference type="OrthoDB" id="8959203at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8034929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="132" mass="15940" checksum="CFE17956C9DDE7A4" modified="2023-09-13" version="1">MESSREFNFNAAFLELSRIEELDSLKKSEIVKVAKHYGIEFQPLMRKDEIKRYVLEYLVDESILPSTVLETAITVPTDNTFELKRLEMEMNKEIRLKEMEREREKEEREIQMQREKEAREHEFRLKQLELGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BZ19</accession>
    <name>A0A9Q1BZ19_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_22172</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BZ19"/>
    <dbReference type="OrthoDB" id="8962223at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8035076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="135" mass="14574" checksum="63EFC2687E08670C" modified="2023-09-13" version="1">MGIQVSHPLKLKFSVMLAQLRHYCLDRIFPLSDSASTGANILIQGVECGLISVPLHKINLKSDLVSVSVIVGVRRTLPVKRISLLLSNGLACGKVTANAIVTNETCNFDNTEQFEKEFPDLFPACAVTGAMSKST</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BZ48</accession>
    <name>A0A9Q1BZ48_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_19284</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8035567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BZ48"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8035567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="96" mass="10491" checksum="8B3AB83874B0DB94" modified="2023-09-13" version="1">MTFVEAVPGPGVATKVTVAADVTVHLQVLQADPLDLHIKLIDDIVARWFRFHLQLHEDLAKDTCIVIDRRYASSLIGTYSTDSRQVSSAGECGSIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BZP9</accession>
    <name>A0A9Q1BZP9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_20331</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BZP9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="78"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="110"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="256"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="16"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="121"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="256"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8036378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="304" mass="33720" checksum="335596B34B1FEBC7" modified="2023-09-13" version="1">MCGRNGDSSPAEDTRTVTTRRTTRATTTTTTRAPTTTLGTTQKPNDFTYPASKYTASSSLNRKATVSSARDGGPMIEFGLIGAVIGGLLLLLVVALLFFFIYRQRRSKTLKDGSRKKDTVDSNVNGESGLTVTMTSLPNYDEEQHDENVKPLYTQVNKKRTDEGPQNTLETHLQNGNKALNATTVIETSRDTEKSKGNEDTEEMVDNMLYESDSAAPSIVHEKPLKDEIPQTNGNRLGNTETTLNKVQSRVTYENVESTTESNTYENQTDRQPESDWGSAYDKLERLDHVIGNQKVIGENVYDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BZY3</accession>
    <name>A0A9Q1BZY3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Mothers against decapentaplegic homolog</fullName>
            <shortName evidence="7">MAD homolog</shortName>
            <shortName evidence="7">Mothers against DPP homolog</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">SMAD family member</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_20534</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the dwarfin/SMAD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BZY3"/>
    <dbReference type="OrthoDB" id="5946219at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071144">
        <property type="term" value="C:heteromeric SMAD protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070411">
        <property type="term" value="F:I-SMAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140416">
        <property type="term" value="F:transcription regulator inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060395">
        <property type="term" value="P:SMAD protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.10:FF:000004">
        <property type="entry name" value="Mothers against decapentaplegic homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.520.10">
        <property type="entry name" value="SMAD MH1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013790">
        <property type="entry name" value="Dwarfin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003619">
        <property type="entry name" value="MAD_homology1_Dwarfin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013019">
        <property type="entry name" value="MAD_homology_MH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017855">
        <property type="entry name" value="SMAD-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001132">
        <property type="entry name" value="SMAD_dom_Dwarfin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036578">
        <property type="entry name" value="SMAD_MH1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13703:SF54">
        <property type="entry name" value="MOTHERS AGAINST DECAPENTAPLEGIC HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13703">
        <property type="entry name" value="SMAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03165">
        <property type="entry name" value="MH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03166">
        <property type="entry name" value="MH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00523">
        <property type="entry name" value="DWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00524">
        <property type="entry name" value="DWB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56366">
        <property type="entry name" value="SMAD MH1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51075">
        <property type="entry name" value="MH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51076">
        <property type="entry name" value="MH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MH1" evidence="9">
        <location>
            <begin position="8"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="MH2" evidence="10">
        <location>
            <begin position="216"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="149"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="194"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8036528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="383" mass="44018" checksum="B5C473B675DCFD9C" modified="2023-09-13" version="1">MFRSKRSALVRRLWRCRVGASPADPHGDSKNSNESEIEKEFKATAQSFLKRLKESQLEQLVEAVESNGGDHTDCVLFPQGEIRLGRRTVDPQVLCCQLFRWPDVKHVGELKRLHFNCKSSRASGDDTNSGHVCCNPYHLSRLCRPESPPPPYSRYPFKRPQEEDEGTPKEGVSASPSSNMERRESTETGNTPTERQEPYAHHSDAEDGETQHRRHWCNVAYWENRTRVGRMYTVFNDHVNIFLELPHGNGFCLGLLHRDNRSEPVLRTRMKIGCGLTLSLESDGVWAYNRSNYALFVNSLTLNWPPSRTFRVSRIEPGFSLKIYDFAKSKLLELDKREPQPPDGPINTRSIQISFIKGWGPRYSRQFITSCPCWLEVILSPQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C0J4</accession>
    <name>A0A9Q1C0J4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transcription elongation factor SPT5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_19784</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8035949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C0J4"/>
    <dbReference type="OrthoDB" id="272624at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040356444" evidence="3">
        <location>
            <begin position="20"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="262"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="141"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="188"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="141"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8035949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="283" mass="29961" checksum="C331C7D42E630F4B" modified="2023-09-13" version="1">MKIFVCVILLLAAVHGAEALQCYHILEYECTAYDYSLLSTCRDVEAENTRFIETCPYSADSCYYSTFYVNEPGQYKLRTIIGGCEYGVINGCYTRSQVATIDPAIEYTLDNIEDDYGVSIQGFQACFCTTDLCNTGLSNQRGVTPQQGGVTSEQRGVTPKQGGVTPKQGGVTPMQGGVTPMQGGVTPKQGGVTPKQGGVTPKQGGVTPKQGGVTPMQGVVTPKQGGVTPKQGGVTPKQRGVASQKGGVTPPRSNSSLLVQTSYMIVGLLALTALLPVIDFAFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C248</accession>
    <name>A0A9Q1C248_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_17670</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8036982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C248"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8036982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="70" mass="7931" checksum="DFF19CA588754BE4" modified="2023-09-13" version="1">MTSWYLYLHSCSCIHTTALPISKDFTAHLLMGWTRTRILWTRTRLGLGGYRTRNWPGTEVTGIGIGSDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C533</accession>
    <name>A0A9Q1C533_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_17118</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C533"/>
    <dbReference type="OrthoDB" id="10070697at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8039408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="121" mass="13959" checksum="F1180490322BDB12" modified="2023-09-13" version="1">MQFRILTYVYKCLHHLAPIYLENLICVYEPGCALRSTDCNYTLVEYVPRRKFGGQSFSVAGPCLWNDIPAHIKESASLQVFQKLLNTQSSFCKPFLLGYLVKGFDDFDLVLYKHQSLLLLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C5X1</accession>
    <name>A0A9Q1C5X1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="16">E3 ubiquitin-protein ligase COP1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HOLleu_15938</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Nanhai2018</strain>
            <tissue evidence="16">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C5X1"/>
    <dbReference type="OrthoDB" id="273771at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16504">
        <property type="entry name" value="RING-HC_COP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000090">
        <property type="entry name" value="E3 ubiquitin-protein ligase RFWD2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042755">
        <property type="entry name" value="COP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44080">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE COP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44080:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE COP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="2 12" id="KW-0853">WD repeat</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="15">
        <location>
            <begin position="48"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="12">
        <location>
            <begin position="447"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="12">
        <location>
            <begin position="533"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="141"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="11"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ8038496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="665" mass="75742" checksum="FED46B7C10234087" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C6J9</accession>
    <name>A0A9Q1C6J9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">N(G),N(G)-dimethylarginine dimethylaminohydrolase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_16789</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DDAH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C6J9"/>
    <dbReference type="OrthoDB" id="10016839at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016597">
        <property type="term" value="F:amino acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016403">
        <property type="term" value="F:dimethylargininase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006525">
        <property type="term" value="P:arginine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000052">
        <property type="term" value="P:citrulline metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045429">
        <property type="term" value="P:positive regulation of nitric oxide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.75.10.10">
        <property type="entry name" value="L-arginine/glycine Amidinotransferase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033199">
        <property type="entry name" value="DDAH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12737:SF9">
        <property type="entry name" value="DIMETHYLARGININASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12737">
        <property type="entry name" value="DIMETHYLARGININE DIMETHYLAMINOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55909">
        <property type="entry name" value="Pentein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8039158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="108" mass="12161" checksum="7948806959FEA974" modified="2023-09-13" version="1">MDDARDVEKLFTFDTVVMRGIPDSLKTGALRQDDLGEVNIGKAREHHQFYMKTMRDKLKLHVELLEPDESLPDCPFVEDCAVVVDGTALITCPGDPSRRPEVRCELSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C6Y6</accession>
    <name>A0A9Q1C6Y6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">46 kDa FK506-binding nuclear protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_13928</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8039812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C6Y6"/>
    <dbReference type="OrthoDB" id="1902587at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.340">
        <property type="entry name" value="Nucleoplasmin core domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041232">
        <property type="entry name" value="NPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057456">
        <property type="entry name" value="Znf_C17orf113"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006580">
        <property type="entry name" value="Znf_TTF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17800">
        <property type="entry name" value="NPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25431">
        <property type="entry name" value="zf-C17orf113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00597">
        <property type="entry name" value="ZnF_TTF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TTF-type" evidence="2">
        <location>
            <begin position="245"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="112"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="217"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="160"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="217"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8039812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="369" mass="41500" checksum="F78C561539192F0F" modified="2023-09-13" version="1">MRIRKSKLGRIENFAAGLTIESGKHYSQSVEDSFHVSMAALELDDDYKSKSRPPIRVMVQHDKKDFLLCSLLYGTLIQQQLDLDFTEGEQVTFFLEGSGVVHLTGSLVQEKPFEVGDEPILGSPPESDEDGMEVSEDESDGKDEEDEEEVPSLQQLAQGDDVESDEEDEEDWIPEQEETSCARVSDSDSFDGRKDLTEQSNGQSMDTLQTWSMAEALNQPSSSSVQKNKSHTCKDGKPKKAKQKVQKKTLEEWLQSFPWSQFDKDSDKVFCGICRKFPKIADKKKTKFVEGCIPFKRETLVVHDKSRCHTLCLKQLQAKKETPVVFGWCSPTDEESDVKQSNLTSTPAVERTEMVTTVDSLDEGTACVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C9Q9</accession>
    <name>A0A9Q1C9Q9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_15446</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C9Q9"/>
    <dbReference type="OrthoDB" id="10261408at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="5"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8040975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="156" mass="17183" checksum="34B0CBCE0BB3B982" modified="2023-09-13" version="1">MSHVCHICHLPFTRGFNLRRHIERFHKQNHGDLEMRGGNSKKGIAKRPHDSSDEGSYGNNSESGTNDGVSEATEEDAMTESSETDDSDADSDTEEADSSEEESSEEDILTDQDGEKDDAPGNVCCPIDTWKALVQMNKAMGIILKQEFKKTTDSAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CAB6</accession>
    <name>A0A9Q1CAB6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Low-density lipoprotein receptor-related protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_12518</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CAB6"/>
    <dbReference type="OrthoDB" id="9990982at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042813">
        <property type="term" value="F:Wnt receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017147">
        <property type="term" value="F:Wnt-protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000241">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050778">
        <property type="entry name" value="Cueball_EGF_LRP_Nidogen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003410">
        <property type="entry name" value="HYR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000033">
        <property type="entry name" value="LDLR_classB_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46513:SF13">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46513">
        <property type="entry name" value="VITELLOGENIN RECEPTOR-LIKE PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02494">
        <property type="entry name" value="HYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00058">
        <property type="entry name" value="Ldl_recept_b"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00135">
        <property type="entry name" value="LY"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63825">
        <property type="entry name" value="YWTD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50825">
        <property type="entry name" value="HYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51120">
        <property type="entry name" value="LDLRB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 9" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040228965" evidence="9">
        <location>
            <begin position="25"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="447"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="6">
        <location>
            <begin position="159"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="6">
        <location>
            <begin position="208"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="HYR" evidence="10">
        <location>
            <begin position="349"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="521"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="525"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8041642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="577" mass="64073" checksum="BDE3ED3C603F4957" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CBZ5</accession>
    <name>A0A9Q1CBZ5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Forkhead box protein F1-B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_13136</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8042145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CBZ5"/>
    <dbReference type="OrthoDB" id="5954824at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009887">
        <property type="term" value="P:animal organ morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20049">
        <property type="entry name" value="FH_FOXF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000071">
        <property type="entry name" value="Forkhead box F1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001766">
        <property type="entry name" value="Fork_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051770">
        <property type="entry name" value="Forkhead_box_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018122">
        <property type="entry name" value="TF_fork_head_CS_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030456">
        <property type="entry name" value="TF_fork_head_CS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46262">
        <property type="entry name" value="FORKHEAD BOX PROTEIN BINIOU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46262:SF2">
        <property type="entry name" value="FORKHEAD BOX PROTEIN BINIOU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00250">
        <property type="entry name" value="Forkhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00053">
        <property type="entry name" value="FORKHEAD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00339">
        <property type="entry name" value="FH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00657">
        <property type="entry name" value="FORK_HEAD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00658">
        <property type="entry name" value="FORK_HEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50039">
        <property type="entry name" value="FORK_HEAD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fork-head" evidence="6">
        <location>
            <begin position="87"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Fork-head" evidence="4">
        <location>
            <begin position="87"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="18"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="28"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8042145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="436" mass="46464" checksum="4C237F5800088664" modified="2023-09-13" version="1">MTISGIMETAISHTNHTQSLGVPAQGGPHSTLNNSAPQLSAGNDHGGPVHQEGKDDQESITLNRAASPIGEKPLAKKSGAGLRRHEKPPYSYIALIVMAIQSSPAKRLTLSEIYQFLMQRFPFFRGPYQGWKNSVRHNLSLNECFIKLPKGLGRPGKGHYWTIDPASEFMFEEGSFRRRPRGFRRKCQALRPHYPMMNSMGPPAHMLGPVGGMEFFAQTQAGGGPGAFGNMTQAGLNQMGMNATGMDGSLGGGMSSCSMPPHLASHMMGTNSPSTNPCAVSSAYMGCPTPASVDAIPVSLQPTTSPNSISNHDGYNSVSGISVDGTYSVSYSSSGWSTGCSNGRYISAKQPNGCSMESSSPLHGIHPQSDHHYMTSHIPDGYDLTHGGQGNAIRGGSVAPPDLVDANCDRKDFVLAAAAQHMQQQQQQTGIQQGTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CC24</accession>
    <name>A0A9Q1CC24_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_13149</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8042157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CC24"/>
    <dbReference type="OrthoDB" id="10253254at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000767">
        <property type="entry name" value="Putative ATP-dependent RNA helicase DHX35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF136">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DHX35-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="5">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="6">
        <location>
            <begin position="113"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8042157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="553" mass="62101" checksum="D410553F4C82C2B3" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CDA2</accession>
    <name>A0A9Q1CDA2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_09333</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8042560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CDA2"/>
    <dbReference type="OrthoDB" id="10053555at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8042560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="107" mass="11714" checksum="34A79F8F43CF93B8" modified="2023-09-13" version="1">MPSGLSALICLGEEEFGDLLPLFEGHLRAGIFLGHILKLRCSSKNSQSVAASQAKAIKFSIQTVPISTDVAQAAKKKETRYKQPFLLGLGPEKKANKFVWTGNYHCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CDV8</accession>
    <name>A0A9Q1CDV8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_10613</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CDV8"/>
    <dbReference type="OrthoDB" id="6141723at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8043502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="118" mass="13353" checksum="CEFDC8336FA1734A" modified="2023-09-13" version="1">MSLAVGQRVDVCLNVAVDDRLINGASCVVKCIQNVQTNQIHTIWVQLDDPSIGKTFRQSTRHLISSDLPSTWTAITRVSRQFRTGRSKNVEILRKQFPLRPPLQKLFTDAKVTTHKVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CFJ3</accession>
    <name>A0A9Q1CFJ3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Neuronal acetylcholine receptor subunit alpha-7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_07240</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CFJ3"/>
    <dbReference type="OrthoDB" id="5975154at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005230">
        <property type="term" value="F:extracellular ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19051">
        <property type="entry name" value="LGIC_TM_cation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040282957" evidence="7">
        <location>
            <begin position="31"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="164"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="217"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="369"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="8">
        <location>
            <begin position="74"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="9">
        <location>
            <begin position="171"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="263"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="263"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="276"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8044478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="396" mass="45555" checksum="5A0F541837315B92" modified="2023-09-13" version="1">MDRKKNRRFFPRTITMLCLFLEMFVRQGEGTTLSGPKRLAEDLFREYGSPIVRPVKDPNKTVEVFFRAKPVWFADFRWTDEYLTWDPDNYNGTEELSVVKRQIWFPDLAASEKDLEAQENVLKKNGVWFVSDIAIEEKLIYYLCCPEPYSEVHYTLHVQRESGFYVINFGVPTLLLSLTTVAVFLLHPESGEKISLCVNNVLALVIFQQLLAATYYFVVVITISFLSVLSTSLTLGMYHHTGSRPLPKLLRLVLFMKESGHQQSSYSRVQAPKNRNQTDAEETRREMTDIADVELSTVTKPDVDYPDNNVDRSAGGRSFWRSSKRGRNSVGASPGLVPVDISKNDVTSCVTDMSFQEEWKKAARRIDKCLFYISLAMMMAAVIFCLFEFMKEKRKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CFL7</accession>
    <name>A0A9Q1CFL7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_10786</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CFL7"/>
    <dbReference type="OrthoDB" id="6581954at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005283">
        <property type="term" value="F:amino acid:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0089718">
        <property type="term" value="P:amino acid import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000175">
        <property type="entry name" value="Na/ntran_symport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037272">
        <property type="entry name" value="SNS_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF037979">
        <property type="entry name" value="Na_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616:SF303">
        <property type="entry name" value="SODIUM- AND CHLORIDE-DEPENDENT GABA TRANSPORTER INE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616">
        <property type="entry name" value="SODIUM/CHLORIDE DEPENDENT TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00209">
        <property type="entry name" value="SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00176">
        <property type="entry name" value="NANEUSMPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161070">
        <property type="entry name" value="SNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00610">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50267">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0915">Sodium</keyword>
    <keyword evidence="8" id="KW-0769">Symport</keyword>
    <keyword evidence="3 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="93"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="188"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="221"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="264"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="297"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="358"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="404"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="433"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="474"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="517"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
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        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
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    <feature type="binding site" evidence="6">
        <location>
            <position position="271"/>
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        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
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        <location>
            <position position="304"/>
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        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
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        <location>
            <position position="370"/>
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        <ligand>
            <name>Na(+)</name>
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            <label>1</label>
        </ligand>
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        <location>
            <position position="373"/>
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        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="374"/>
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        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="133"/>
            <end position="142"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR600175-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR600175-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8043609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="586" mass="66079" checksum="2B696C074D9F8189" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CH55</accession>
    <name>A0A9Q1CH55_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Heat shock protein HSP 90-alpha 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_07402</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the heat shock protein 90 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="5426351at2759"/>
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        <property type="component" value="Chromosome 3"/>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:ATP hydrolysis activity"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="entry name" value="HATPase_Hsp90-like"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="FunFam" id="3.30.230.80:FF:000001">
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    <dbReference type="FunFam" id="3.40.50.11260:FF:000001">
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    </dbReference>
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="Histidine kinase/HSP90-like ATPase" evidence="10">
        <location>
            <begin position="40"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="225"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="703"/>
            <end position="734"/>
        </location>
    </feature>
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        <location>
            <begin position="231"/>
            <end position="247"/>
        </location>
    </feature>
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        <location>
            <begin position="257"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="703"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="112"/>
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        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
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    <feature type="binding site" evidence="8">
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            <end position="114"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
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    </feature>
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        <ligand>
            <name>ATP</name>
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        <ligand>
            <name>ATP</name>
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    <feature type="binding site" evidence="8">
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            <position position="404"/>
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        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
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            <dbReference type="ARBA" id="ARBA00004496"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00008239"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
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        <source>
            <dbReference type="PIRSR" id="PIRSR002583-1"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00387"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAJ8044615.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="734" mass="84637" checksum="3308F7246D786F45" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CHF7</accession>
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        <submittedName>
            <fullName evidence="3">N-lysine methyltransferase KMT5A-B</fullName>
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        <name evidence="3" type="ORF">HOLleu_08340</name>
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        <name type="scientific">Holothuria leucospilota</name>
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        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR33480">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33480:SF1">
        <property type="entry name" value="TYR RECOMBINASE DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
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        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS50280">
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    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SET" evidence="2">
        <location>
            <begin position="24"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
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    <feature type="region of interest" description="Disordered" evidence="1">
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="859"/>
            <end position="888"/>
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        <location>
            <begin position="160"/>
            <end position="169"/>
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    </feature>
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        <location>
            <begin position="859"/>
            <end position="871"/>
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    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="878"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8045348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="955" mass="108533" checksum="47D3E6459FFF0C8F" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CI71</accession>
    <name>A0A9Q1CI71_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_08887</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CI71"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8045802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="124" mass="13718" checksum="33F28E20079EF463" modified="2023-09-13" version="1">MPHIERKNPIVFGRGQRSPGVTGGQTLKTLLTQYLKMRYGERKNPIIFGGGERSSGVTGGQTLKTLLTRYLKMRYGKRKNPIIFGGGERSSGVTGGQTLKTSLTRYLKKGSSDSSQTWYIDAPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CKA4</accession>
    <name>A0A9Q1CKA4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Selenide, water dikinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_04884</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CKA4"/>
    <dbReference type="OrthoDB" id="409395at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004756">
        <property type="term" value="F:selenide, water dikinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016260">
        <property type="term" value="P:selenocysteine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02195">
        <property type="entry name" value="SelD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.650.10:FF:000010">
        <property type="entry name" value="Selenide, water dikinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="entry name" value="PurM-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="entry name" value="PurM-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004536">
        <property type="entry name" value="SPS/SelD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00476">
        <property type="entry name" value="selD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10256:SF0">
        <property type="entry name" value="INACTIVE SELENIDE, WATER DIKINASE-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10256">
        <property type="entry name" value="SELENIDE, WATER DIKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036407">
        <property type="entry name" value="Selenphspht_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="PurM C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="PurM N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0711">Selenium</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PurM-like N-terminal" evidence="6">
        <location>
            <begin position="2"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="PurM-like C-terminal" evidence="7">
        <location>
            <begin position="124"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8046265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="312" mass="33818" checksum="8DCC08AD87B7B210" modified="2023-09-13" version="1">MDCSVTPLRHGGLSLVQTTDFFYPLVDDPYMQGKIACANVLSDMYALGITQCDNMLMLLGVPQKYTDKEREAVIPLIIAGFKDAARDAGTNITGGQTVLNPWCIIGGVATSAVLAGDIIIPHAEPGNVLVLTKPLGTQVAVNAKQWKEQNNERYKKFMSSNQLTGADIDRAYQEAMFNMARLNKKAATLMRHFNARGATDITGYGILGHAQNLAKYQSNEVKFVIHNLPVIAKMSTVAKASGNLFQLLQGYSAETSGGLLIAFPRDQAAAFCRAMDTQDGNPSWIVGVVDVGDRTATMSPKPRVIEVTTKME</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CKG3</accession>
    <name>A0A9Q1CKG3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_04819</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CKG3"/>
    <dbReference type="OrthoDB" id="10671648at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="33"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="315"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8046209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="390" mass="44441" checksum="3E140DB59FB35E49" modified="2023-09-13" version="1">MASTSLYNESSIPEISSLAPTVPTSVEKEELKWIWFVVPFVTALIIFFCFEFTVILLLCRRNRYRKAHLLFTDEDGQLMYSTDRINENVVLSGYRGIRTSISSSHDIRGHFAPIEYCSMCGNASSRDGFMYDTSTFRGYRDHRRHTRDGMLLYSEPLPDHRVLTSTMNDTANHDNTEYAREPSYNPRQVSRAFRELSSVSDIYTARSTDAEDDTYTPRFTPTVSRSISEDLADSESVPQSIERNGGELATQAQHYDSVYIHHHGNSLDSCTSTTSNISDHQLLEGKPNGNILVKSNGHRNFLNSYFVNRVRPNNAPHSKDAINHVGGESVTTTSVEREQIKNDTDRNSFVKHYEYSRPLHLQEEEDTRHFIESYLASSCENVSVTAYSHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CL34</accession>
    <name>A0A9Q1CL34_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Titin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_05432</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10036029at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050991">
        <property type="entry name" value="ECM_Regulatory_Proteins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46708:SF2">
        <property type="entry name" value="FIBRONECTIN TYPE-III DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46708">
        <property type="entry name" value="TENASCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="3">
        <location>
            <begin position="156"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="3">
        <location>
            <begin position="273"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="3">
        <location>
            <begin position="393"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="3">
        <location>
            <begin position="761"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="3">
        <location>
            <begin position="888"/>
            <end position="985"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="3">
        <location>
            <begin position="987"/>
            <end position="1077"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1633"/>
            <end position="1668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1639"/>
            <end position="1668"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8046670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1668" mass="185951" checksum="A76EF2EFD3569EB5" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CL59</accession>
    <name>A0A9Q1CL59_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_05133</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CL59"/>
    <dbReference type="OrthoDB" id="7315948at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47018">
        <property type="entry name" value="CXC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47018:SF1">
        <property type="entry name" value="TESMIN_TSO1-LIKE CXC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="128"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8046459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="399" mass="45723" checksum="04959536C057AD7E" modified="2023-09-13" version="1">MELTILLLIRSFREANFNLYCDSLSELMPYFFANNNTNYARWLSIHLRDMVTLEEKHPQVAQEFHNGKFVIHKSNREFSGMAIDQAHEQANAVIKADGGAIGIKEDPSALRRWMVAGPEVSHLVAQYEKASGAQETSQQNSHHEQNQRAQNAFLERVEKLFNVMTDMGNPFQEDSRDLMSLDTKDIAHPSAAKLISTHYEKGKARFEEFMKGLEEKVSIFYEPIKKNRIDFFCQEPVSSEISKEKAIKKDCQLFSKLFISCQNRECDLNEFFRHENHPLPAALSDDGKLHLCQKSQLATILEALVTIPDKEPEADCIIIYGSALVNSLPPRTSKTFEEYSALDVLPTVQLYSIRYKRTDNAFNVYHSFSLKSETRSKRGHGVRRRVTSITKVVKLPTRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CLD5</accession>
    <name>A0A9Q1CLD5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Carboxylic ester hydrolase</fullName>
            <ecNumber evidence="3">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_05694</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the type-B carboxylesterase/lipase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="3"/>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CLD5"/>
    <dbReference type="OrthoDB" id="19653at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019826">
        <property type="entry name" value="Carboxylesterase_B_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45570">
        <property type="entry name" value="CARBOXYLIC ESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45570:SF1">
        <property type="entry name" value="CARBOXYLIC ESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00122">
        <property type="entry name" value="CARBOXYLESTERASE_B_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carboxylesterase type B" evidence="4">
        <location>
            <begin position="1"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8046868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="379" mass="43468" checksum="5B5A87B3507709A4" modified="2023-09-13" version="1">MLDQRLALQYVKDNIANFGGDPNKVTLAGQSAGAQSIGLHLLANDSEPLFQQTYMISNPFGLPYKLPEDALKLAEYFAEALGCPHLDLSCFREKEADEVLAASESAALNIINPLELFQFFEQWNPTVGEGEYPEEPVMAYEEGNFQRKPTIAGTVTEEGRAYVYSVFTNEVPWWMYRVIIRLLVPKHSQYVIDYYPPERLAPDQREMLNLLVTDYLFLCATSHVSQMMVKYDVDVYNYANDASMSFSQFWEQYDICKGYPCHGGDLVYIFRTAPLVNETFTPGEQDMVEDMTSYIGNFVRTGNPNTAFNTEIRKMQKKNLPYWPKVGDKPTNTYTMYFSACSNSVVSNYRKEYCDAFDEYGYYKIPFLNSYPESDKCQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CLQ7</accession>
    <name>A0A9Q1CLQ7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein artemis</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">DNA cross-link repair 1C protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HOLleu_06584</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Nanhai2018</strain>
            <tissue evidence="15">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA repair metallo-beta-lactamase (DRMBL) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CLQ7"/>
    <dbReference type="OrthoDB" id="262529at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035312">
        <property type="term" value="F:5'-3' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.15.10:FF:000018">
        <property type="entry name" value="DNA cross-link repair 1C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12650">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011084">
        <property type="entry name" value="DRMBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23240">
        <property type="entry name" value="DNA CROSS-LINK REPAIR PROTEIN PSO2/SNM1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23240:SF8">
        <property type="entry name" value="PROTEIN ARTEMIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23023">
        <property type="entry name" value="Anti-Pycsar_Apyc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07522">
        <property type="entry name" value="DRMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="14">
        <location>
            <begin position="2"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="374"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="412"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="527"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="417"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="453"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="481"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="527"/>
            <end position="548"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00042677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ8047553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="728" mass="82225" checksum="2101B8713C4F9DF3" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CNW0</accession>
    <name>A0A9Q1CNW0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GIY-YIG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_06972</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CNW0"/>
    <dbReference type="OrthoDB" id="10025388at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="CDD" id="cd10442">
        <property type="entry name" value="GIY-YIG_PLEs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000305">
        <property type="entry name" value="GIY-YIG_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035901">
        <property type="entry name" value="GIY-YIG_endonuc_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82771">
        <property type="entry name" value="GIY-YIG endonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50164">
        <property type="entry name" value="GIY_YIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GIY-YIG" evidence="1">
        <location>
            <begin position="120"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8047854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="216" mass="24544" checksum="212B132B4D64B4A4" modified="2023-09-13" version="1">MYPSNHPRSCTNSIPSHNFYGPDGFVPTTRISLKFPSRLFRIQGIDRASALAPKTDHTPTRRIPLVLSFHPSVTPIVRAIYRNVETLRHDPCTRDHFSEPPPPPSQNFSLQPWEMQYLPGANVVYVLVCKRCNILYVGETKRRLADRLTEHLRSIKQNLPGFPVVTHFNAPSTCSIRDLMVSAAISCRGSDHDRLAAENRLIMKLGTLYVPTWSER</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CQV7</accession>
    <name>A0A9Q1CQV7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Angiopoietin-related protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_02816</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CQV7"/>
    <dbReference type="OrthoDB" id="7250310at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00087">
        <property type="entry name" value="FReD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19941">
        <property type="entry name" value="TIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050373">
        <property type="entry name" value="Fibrinogen_C-term_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036084">
        <property type="entry name" value="Ser_inhib-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025615">
        <property type="entry name" value="TILa_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040941">
        <property type="entry name" value="GGGWT_bact"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143">
        <property type="entry name" value="FIBRINOGEN/TENASCIN/ANGIOPOEITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12714">
        <property type="entry name" value="TILa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00186">
        <property type="entry name" value="FBG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57567">
        <property type="entry name" value="Serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040310022" evidence="2">
        <location>
            <begin position="27"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="4">
        <location>
            <begin position="49"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="282"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="4">
        <location>
            <begin position="321"/>
            <end position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8049882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="575" mass="65739" checksum="3AEC930A00F38206" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CR44</accession>
    <name>A0A9Q1CR44_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_02308</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CR44"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8049525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="77" mass="9015" checksum="D6393ECB39F2904C" modified="2023-09-13" version="1">MKVDRKRYYTDDAMLPRPERDKVEIEMNSLEGLFQSLNELIEMEVDSPEETQLGTLQADHYTLVKKVGDVIREIKSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CSW6</accession>
    <name>A0A9Q1CSW6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_02962</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CSW6"/>
    <dbReference type="OrthoDB" id="10066002at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31025">
        <property type="entry name" value="SI:CH211-196P9.1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31025:SF9">
        <property type="entry name" value="SI:DKEY-286J15.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8049974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="259" mass="29282" checksum="711E69F4AD9CF88A" modified="2023-09-13" version="1">MFLLFPFLVNCIDDKEAFDLLTPDVPKELIPQIGPRLKFSAKFRNYQKIEQDFRIIYGEETADSFITKGDTCAEKILTFCNSQEISWKPYFSIQLSDDHSLLALQMLPLVLPSGLVRNKRGKNLRASRAEALSAFIDIQPLCTLPLQVGTNLPHYLETCQAERQKTQPYILVLGDRLKPSQTFVVPDGVALEAGTLLKACDICFKAAYILDVHYPKQCQTTWEFIQKYFFQLGDGKGKSITSPGVRTLRTFLEASVESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CTJ5</accession>
    <name>A0A9Q1CTJ5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Reverse transcriptase RNase H-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_03515</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CTJ5"/>
    <dbReference type="OrthoDB" id="7687667at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09274">
        <property type="entry name" value="RNase_HI_RT_Ty3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041373">
        <property type="entry name" value="RT_RNaseH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17917">
        <property type="entry name" value="RT_RNaseH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Reverse transcriptase RNase H-like" evidence="8">
        <location>
            <begin position="14"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="204"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8050349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="443" mass="51043" checksum="134D1E783D83884A" modified="2023-09-13" version="1">MTKPPILHLPRSTGTFNLYSDTSRIATGSYLTQTVEDKEYIVGYYSKVLPPACQKYSFTELEMFGLLINITAFKHMLKPNEFNAFVDHSSIVQILQSKDEHSTSRLRKLLQKLSDYSFKIGYKKGSDLVLADFLSRNPNYSHSEIDEVILIAFCLFASELLIEGKEPVCNPIVTRSYAKKHGIDLPDLFPDGLKRKETVTVPTQSCGENSQTSGNVANPPSDSGITRRQFPLHRPMHLPDHGHQRNEVRDAQPINTRPPPHVAHEPRLVDRETDDDIRDPPELYRVPKPLISKVDGLVTRHVPKQAELDKMMNIIKRKIIRDYNLPIDVRQLKTEQETSSYFKPVYDYLAYDILPKDKKAAKSVQMKAEQYILCNGILFRLFFHDKDEEFTLQLAIPESLSETIISRYHDNWLCNHQAVVRTYVTVRVTTVRMNWHLVEKHLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CTY6</accession>
    <name>A0A9Q1CTY6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_04375</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CTY6"/>
    <dbReference type="OrthoDB" id="6693376at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8050978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="186" mass="21415" checksum="CA62E0A67C934B97" modified="2023-09-13" version="1">MYVPRPPSPTFEYGLTPLIPRGNISFTRESLFLGNLPKRLVIGCVDSEASNGSYNRNPFNFHHHDLNFIALYVDGEQLPWKPLRPNFGDGQYIMAYQTLFSGLNTMFSDKGKHIDRYDYGHGYTLYAFDMTPDLANGGHFNLRKNGNVRLEMQFDHTLMRTVNVIVYAEYDAIGEIDKTRNVFIDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1GZ77</accession>
    <name>A0A9Q1GZ77_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">COP9 signalosome complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_29136</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CSN2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1GZ77"/>
    <dbReference type="OrthoDB" id="194139at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008180">
        <property type="term" value="C:COP9 signalosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.570:FF:000001">
        <property type="entry name" value="Putative cop9 signalosome complex subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050871">
        <property type="entry name" value="26S_Proteasome/COP9_Components"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058796">
        <property type="entry name" value="COPS2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10678">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 11/COP9 SIGNALOSOME COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25983">
        <property type="entry name" value="COPS2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00753">
        <property type="entry name" value="PAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0736">Signalosome</keyword>
    <feature type="domain" description="PCI" evidence="8">
        <location>
            <begin position="193"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8029682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="382" mass="44638" checksum="27B7AE2263C65B2C" modified="2023-09-13" version="1">MEKGDWGFKALKQMIKINFKLGNYDEMMNRYKQLLTYIKSAVTRNYSEKSINSILDYISTSKNMTLLQDFYETTLLALKDAKNDRLWFKTNTKLGKLHYDREEYNKLARILKQLHQSCKTEDGSVDLKKGTQLLEIYALEIQMYTAQKNNKKLKALYEQSLQIKSAIPHPLIMGVIRECGGKMHLRECEYEKAHTDFFEAFKNYDESGSARRTTCLKYLVLANMLMKSDINPFESQETKPYKNDSEILAMTNLVSAYQNNDITEFEKILKRNRKNIMDDLFIREHIEDLLRNIRTQVLIKLIKPYTRIHIPFISRELNIDVTEVESLLVQCILDHTIYGRIDQVNQVLELNEKSKVSARYAALDKWTAQLGSLHQSVANNLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H389</accession>
    <name>A0A9Q1H389_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_23996</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H389"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040502782" evidence="1">
        <location>
            <begin position="20"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8033672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="79" mass="8599" checksum="9F2668333B2331D8" modified="2023-09-13" version="1">MKTFVFFVVLACLFTIALALKITELTNVVAQDTRHQVARQRQFVISTVKARAALIMDGAAGKMCTAPVMIAQITEVCIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H6M1</accession>
    <name>A0A9Q1H6M1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_22233</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H6M1"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8035114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="69" mass="7287" checksum="6C23ABE5AFDF5068" modified="2023-09-13" version="1">MIPKAEKLEKKQEAKTSLVSATVTSADASSLSNVCATSGQRSFGVTRGQNVKILSTLYLKKGNCEGSHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H6X5</accession>
    <name>A0A9Q1H6X5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_21597</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H6X5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040514602" evidence="1">
        <location>
            <begin position="24"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8034665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="263" mass="29022" checksum="D397C6FCC83B6BB6" modified="2023-09-13" version="1">MSNLRTLIISCFAAVKFFLPAEGTVLPDLITRDQHVVLIVYGQVVATSFEESLTCAQEESFETRPNTLTFQIPHLSSVPGGLPQLGLVLSRYLSPKTNVIDPNFCITPKIPADELKYTSNHSCVGLFFSHGEDLKLKMSGEVSGSCRRPPDEVVTDFAKSTDELLISRRPPEFSVCDRLGDIDVDFCSAWRSYGEDITVYGSNVCQCWNHARNITSCQLSISVIEMSSKLRDTLALPQGDEPEAIEAEMKRAFHGIGLISPCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H7C8</accession>
    <name>A0A9Q1H7C8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_19654</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8035849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H7C8"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="246"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="292"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8035849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="309" mass="34911" checksum="0864B152C56E5804" modified="2023-09-13" version="1">MRRQLGFGDQEPAQDPAPRKSKLSLNRQATEKESLLPVDVECLDRFKVAAESKKWSPFPRKQCSDFKLEEKEWKDCFKSPRIPVGAVDHLKNAGAMDPKGKFKSSATKKSNQAFAGIDKAARAGMKFLSALLLVAEVLSKSFRQTGEETVSRKDTGTVVNILGPLSRLIFDQFSCIAVRSVRERRDLVMDALILPSQDIKRRFLELPLSGEDIFGGLFDSHLQTEVKRQKDMKKADFRIPSSFYNRWSPDRKSRQRPRSSSDCPSNPHRSYRPRPTPPSRGGRAPFLSRQLSRGGSRSTSRGRGNFQKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H8Z3</accession>
    <name>A0A9Q1H8Z3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Opsin-VA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_17755</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H8Z3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00637">
        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24243">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24243:SF208">
        <property type="entry name" value="PYROKININ-1 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="62"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="105"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="164"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="222"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="10">
        <location>
            <begin position="1"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8037035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="302" mass="34253" checksum="4AEA5B6D2716664B" modified="2023-09-13" version="1">MAFIFIVWKEPEMRNDMNVILLNLSVADLLFLLVGVGEKFWGVLTSPLYRDKLFYRKPGECLTITFLLDLVSFTSLLLVSLLSVERCFVICQPMKHLRTKSLKKTSIHFGLAWFSACVVAAFTFSFYEFATYCVKWPNKAPYTNYPIKAAGCKPRYGTSGVTNAFILEIGPFFVAMVVNVVCFVKIMSKLYQRLPTSSEEGYENGTVSTQHRYSAHAATRMLLINGIAFFILATPFHVEFALEFFPLFSFDLPQYVKATCTMLLYANSAVNPYIYGITNKCYRQAYRNILCSKSSTSNVTSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H928</accession>
    <name>A0A9Q1H928_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_14627</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H928"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8040362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="154" mass="17858" checksum="3E65823FB178F110" modified="2023-09-13" version="1">MPHIKRKDPIVFGGGQRSSGVNRGQTLKTLLARYLERYENQFKLTTLLERMRCGERKNPIVFGGGQRSSGVTRGQNLKTLFTRYLKVGSLDRFHTKYVDVLWCKEEPYRFWWRSKVIWGHRGSKSEKLVYMILQGCSGKPQSLLSILIGQNLFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HBB2</accession>
    <name>A0A9Q1HBB2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Translationally-controlled tumor protein-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_13793</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TCTP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8039705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HBB2"/>
    <dbReference type="OrthoDB" id="10248936at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.10">
        <property type="entry name" value="Metal Binding Protein, Guanine Nucleotide Exchange Factor, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011057">
        <property type="entry name" value="Mss4-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011323">
        <property type="entry name" value="Mss4/transl-control_tumour"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034737">
        <property type="entry name" value="TCTP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018105">
        <property type="entry name" value="Translational_control_tumour_p"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11991">
        <property type="entry name" value="TRANSLATIONALLY CONTROLLED TUMOR PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11991:SF0">
        <property type="entry name" value="TRANSLATIONALLY-CONTROLLED TUMOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00838">
        <property type="entry name" value="TCTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01653">
        <property type="entry name" value="TCTPROTEIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51316">
        <property type="entry name" value="Mss4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51797">
        <property type="entry name" value="TCTP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TCTP" evidence="2">
        <location>
            <begin position="1"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8039705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="178" mass="20747" checksum="887A5026E6C0B974" modified="2023-09-13" version="1">MVVYQDIISGDEFFYGSHPFTLVDDLYYEVYGRYILIRSGGVKDTELGGNRFAKLVPAEQPAEQPEKLEEVIDLVEECQLQNCPHISQEKYFPEYLKPYTQTLIAYYEKQGKEKADIVKDKLNQFRVILREKFKSDNFQFFEGRSHPSEGMIALVGHKEKDGKEIPYMILFKDGLKEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HCD2</accession>
    <name>A0A9Q1HCD2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Nipped-B protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_15282</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the SCC2/Nipped-B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="418242at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090694">
        <property type="term" value="C:Scc2-Scc4 cohesin loading complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061775">
        <property type="term" value="F:cohesin loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140588">
        <property type="term" value="P:chromatin looping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034087">
        <property type="term" value="P:establishment of mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071169">
        <property type="term" value="P:establishment of protein localization to chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990414">
        <property type="term" value="P:replication-born double-strand break repair via sister chromatid exchange"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23958">
        <property type="entry name" value="SCC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026003">
        <property type="entry name" value="Cohesin_HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024986">
        <property type="entry name" value="Nipped-B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033031">
        <property type="entry name" value="Scc2/Nipped-B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21704:SF18">
        <property type="entry name" value="NIPPED-B-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21704">
        <property type="entry name" value="NIPPED-B-LIKE PROTEIN DELANGIN SCC2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12765">
        <property type="entry name" value="Cohesin_HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12830">
        <property type="entry name" value="Nipped-B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Sister chromatid cohesion C-terminal" evidence="8">
        <location>
            <begin position="1695"/>
            <end position="1877"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="109"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1076"/>
            <end position="1127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="2028"/>
            <end position="2201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="149"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="199"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="226"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="261"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="299"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="310"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="386"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="405"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="419"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="454"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="494"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="522"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="1084"/>
            <end position="1102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1106"/>
            <end position="1119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="2059"/>
            <end position="2072"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="2096"/>
            <end position="2106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="2108"/>
            <end position="2128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="2134"/>
            <end position="2154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="2155"/>
            <end position="2167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="2187"/>
            <end position="2201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU364107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8040860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="2201" mass="246854" checksum="BB6B619746CE018C" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HD67</accession>
    <name>A0A9Q1HD67_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_12607</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HD67"/>
    <dbReference type="OrthoDB" id="6073759at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8041715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="135" mass="15969" checksum="11B3DC7889E9B473" modified="2023-09-13" version="1">MRIELMETLDLADIWRERNPFQKHFTWSSNVSPDIHCRLDFFLIFRNLVSQVTRNFFSPSLQSDHFIISLDITIFAGRRGPSFWKFNCSFPADENYTLLIQDIIVAINSTEQFLNASKKWEFIKFKVGMVSMNYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HDE6</accession>
    <name>A0A9Q1HDE6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Carbohydrate sulfotransferase 15</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_12879</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HDE6"/>
    <dbReference type="OrthoDB" id="8068875at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050659">
        <property type="term" value="F:N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019319">
        <property type="term" value="P:hexose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052654">
        <property type="entry name" value="CS_Sulfotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15723">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15723:SF0">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="domain" description="Sulfotransferase" evidence="2">
        <location>
            <begin position="244"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8041940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="425" mass="50491" checksum="0E466CEEB9F1BC52" modified="2023-09-13" version="1">MIKITQNNRRLPIFFVGAALCFVILFSLHTYYPNIRLQEYQESMGFTHFYDESKAREWLELQNAFDVNISGSESISRGKPLAKEWRPYVEELFERLPSNFSSSHRNPCWYEDGTLRCLPYFYQLGSYKCGTTDLWDKVVQHPHVIRVAKEPHWWAWRRFGYTSLPIHRDLVLKIRNITGDRDDHSLQWYLNLYSYQATEAIRQNRKLVFGDASISNLWGLGDYVWEKKFPALEEPPFFLASVIRTFQPNAKITAILRDPVEKLWTTYMLGDRYKKMSFKKHFENLLKASAACEKKHSFKYCAFMYRNFPSSILSNHLYQGIYYVYLKEWVDAFGADNIHVITLEDWKNDTVSELNSVFQYLDLDPLPKETMASIVERSAKNVNKKAKSRNMVMSQQMRRRVKSFYRPWNEKLVELLGDQKFMWGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HDU5</accession>
    <name>A0A9Q1HDU5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Sialin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_13642</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8042554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HDU5"/>
    <dbReference type="OrthoDB" id="10068056at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006820">
        <property type="term" value="P:monoatomic anion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050382">
        <property type="entry name" value="MFS_Na/Anion_cotransporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11662:SF399">
        <property type="entry name" value="FI19708P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11662">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="46"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="115"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="141"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="49"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8042554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="201" mass="22009" checksum="88784896CDEE59AE" modified="2023-09-13" version="1">MTDEGESSCLDDKDTNLNHTETALLLPQPDLHVNTYSSGKKQCIPYRYIVTLLTFAGFFNVYAMRVNLSVALVAMVNSTVNPEPNVSDACPLLSNHTSEAKEGPFLWDPTIQGQILASFFYGYIVTQIPGGFLAGKYGAKWPFGIGVLCTTVFTLATPYVSTVSPYALMVLRVLEGLGEVSCKICPNLSVTCFCEFLIFFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HE41</accession>
    <name>A0A9Q1HE41_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Prestin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_04801</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HE41"/>
    <dbReference type="OrthoDB" id="288203at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07042">
        <property type="entry name" value="STAS_SulP_like_sulfate_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="entry name" value="STAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SLC26A/SulP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001902">
        <property type="entry name" value="SLC26A/SulP_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00815">
        <property type="entry name" value="sulP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11814">
        <property type="entry name" value="SULFATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01740">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00916">
        <property type="entry name" value="Sulfate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SpoIIaa-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="186"/>
            <end position="211"/>
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    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="232"/>
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="5">
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            <begin position="262"/>
            <end position="280"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="292"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="341"/>
            <end position="360"/>
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    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="381"/>
            <end position="400"/>
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    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="420"/>
            <end position="438"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="475"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="domain" description="STAS" evidence="6">
        <location>
            <begin position="530"/>
            <end position="688"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8046197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="698" mass="77040" checksum="650A5130B71DA81C" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HG78</accession>
    <name>A0A9Q1HG78_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_01097</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HG78"/>
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        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33480">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33480:SF1">
        <property type="entry name" value="TYR RECOMBINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8048687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="107" mass="12353" checksum="36803BE197192C03" modified="2023-09-13" version="1">MGHDIETHREYYRLPQETILLAKMSKLFNLASEGKLHKFKGKSLEDVAVSPEEVLSTEVAEEEGGRRRTRRRKMVKGKVQEEKKEGQGQVSHKEITTDGQYNKMTFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HGJ0</accession>
    <name>A0A9Q1HGJ0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_01603</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HGJ0"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8049047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="50" mass="5741" checksum="14CF3C20BF707ACA" modified="2023-09-13" version="1">MPHIQRKNPIVFGGGQRSFGVTKGQNLKTLFTRYLKVGSLDRFHTWHVDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HHG9</accession>
    <name>A0A9Q1HHG9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_03188</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HHG9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8050112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="124" mass="13882" checksum="63FF0D8D7F441D7C" modified="2023-09-13" version="1">MHYDEKMNPIISGGGQRSSGVTGGRKLKTLLTRYLHMHYDEKMNPIISGGGQRSSGVTGGRTLKTLLTRYLHMHYDEKVNPINSGGGQRTSEVIGGQTLKTLFTRYLQVGSFDRFNTQYVDALW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HIK9</accession>
    <name>A0A9Q1HIK9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_00245</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HIK9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040297705" evidence="1">
        <location>
            <begin position="22"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8048074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="333" mass="37454" checksum="9BCCBD39F6B711B5" modified="2023-09-13" version="1">MATKLLSGLLFLLILVSEGWASYDVKVSDVFFDGGFLFFSVKRAKLQQRLDEINSQFGDNNFVLLMPSEPVLQDVIASDEHVIMVTFGTMEVVAPENGMTNSTTVEGLSIQIPYLTVDNDDALDITFQVDTFISEVGPHIQADLIPLSATVADLFVLEETGNTTKIQFQNGELQLSLFGEYMESRPLNEEDGNTHLKEYNKHVQTSLSGPTFSICDQSPDVTLCDIYKHPQYNPVLEEEGTNVCQYWEPIRDDIQRWDSTFQVVSISQAMRELLLLDEDQDVLQAELQSGTFSVKLVFPCNGANARFHLLPYQTFGTSLVVACGLKFWWNMFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HJ83</accession>
    <name>A0A9Q1HJ83_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_06820</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HJ83"/>
    <dbReference type="OrthoDB" id="2019384at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050685">
        <property type="entry name" value="LDLR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24270">
        <property type="entry name" value="LOW-DENSITY LIPOPROTEIN RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24270:SF62">
        <property type="entry name" value="LOW-DENSITY LIPOPROTEIN RECEPTOR-RELATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040481149" evidence="8">
        <location>
            <begin position="16"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8047745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="157" mass="17613" checksum="DBFB5991F124DCFE" modified="2023-09-13" version="1">MFVLHHLCVLTYVFSKGKNTPCFGTPYEFHCDEVNNSCIFSYFRCDGIIDCPNGADEDPAISEADWAKMCERENRSSKFKPFKMATCNNTMCITECDLCDGYYLDCVDGSDEDPALCNGDNKPQLVGDCTLYRQSYPFPIGEPRGSPPTLSPHFTIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HJQ5</accession>
    <name>A0A9Q1HJQ5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Contactin-4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_06669</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HJQ5"/>
    <dbReference type="OrthoDB" id="5982258at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170:SF6">
        <property type="entry name" value="CONTACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170">
        <property type="entry name" value="PROTEIN SIDEKICK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="62"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="216"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="300"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8047626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="349" mass="38437" checksum="B21F64DE3CD66D77" modified="2023-09-13" version="1">MIMENNLYNVTEEGSLLVSYPPKNLNGSSYRCIASNSFGTVMSRAATLEIAYVDTFPVPSAPRYKTLNAKLGTCITCNPPEAYPGIHADWKAGVFTDINSNDRIREMIDGRLCFAYTTTGDRGTYYCVVSNKYDKGEATKRSPPLSFDVINVLDLHQLLNGPDGTLIYPNSTQLKYNQELRIPNAQPQDSGEYQCIGASRGYSETVSRYLTVLAPPNLVSGLKDAKVDSGSTVTMQCTADDDGVEFSWYHNTRLLNSSMKRPRISEDNTLTIDNVMVQDSGIYQCAARNEHGMSLSTAQLSVEEQEPTEMKPTESEIDQPDSRSPGNTVGDTFVYIFAAMVSAALLEIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0Y8T2</accession>
    <name>A0A9Q0Y8T2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein NLRC5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_44513</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000952">
        <property type="protein sequence ID" value="KAJ8017833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0Y8T2"/>
    <dbReference type="OrthoDB" id="427518at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312">
        <property type="entry name" value="NACHT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312:SF2">
        <property type="entry name" value="NUCLEOTIDE-BINDING OLIGOMERIZATION DOMAIN-CONTAINING PROTEIN 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040243743" evidence="2">
        <location>
            <begin position="20"/>
            <end position="1029"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="473"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="761"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="785"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="domain" description="NACHT" evidence="3">
        <location>
            <begin position="591"/>
            <end position="710"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8017833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1029" mass="117284" checksum="3E0A25E58E74BEA2" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0Y8Z8</accession>
    <name>A0A9Q0Y8Z8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_44216</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000716">
        <property type="protein sequence ID" value="KAJ8018023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0Y8Z8"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8018023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="56" mass="6357" checksum="F7DA0E8B6C634A7A" modified="2023-09-13" version="1">MLEVLCTQLHWQLSHPTRGQCWQACFVMESQPPKPSQMATSSLTEMELCFATSLTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0Y904</accession>
    <name>A0A9Q0Y904_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">THAP domain-containing protein 10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_44187</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000696">
        <property type="protein sequence ID" value="KAJ8018038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0Y904"/>
    <dbReference type="OrthoDB" id="5967653at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.210.20">
        <property type="entry name" value="THAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006612">
        <property type="entry name" value="THAP_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038441">
        <property type="entry name" value="THAP_Znf_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31751:SF42">
        <property type="entry name" value="PROTEIN CBG10204"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31751">
        <property type="entry name" value="SI:CH211-108C17.2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05485">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00692">
        <property type="entry name" value="DM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00980">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50950">
        <property type="entry name" value="ZF_THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="THAP-type" evidence="7">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="93"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="293"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="114"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="190"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="219"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="296"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="330"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8018038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="601" mass="67186" checksum="0334BBAA2C838E71" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YAK3</accession>
    <name>A0A9Q0YAK3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_42246</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000045">
        <property type="protein sequence ID" value="KAJ8019267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YAK3"/>
    <dbReference type="OrthoDB" id="10030726at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8019267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="101" mass="11430" checksum="D59E6FFA73F5AE31" modified="2023-09-13" version="1">MNNGTTYQLQDLSNLNKPAKILHYNRLKPFCSPYNLGEGGDGNVNIPMQVQVPRLNLQMEIPEDQSEPQAPTTNVSKDNKVTRVGRKVKLPSYLNDYELDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YC49</accession>
    <name>A0A9Q0YC49_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_44734</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010001158">
        <property type="protein sequence ID" value="KAJ8017684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YC49"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8017684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="64" mass="7160" checksum="9153202D35D8CD6F" modified="2023-09-13" version="1">MESSREFNFNAAFLELPRTLALSLFARGTSSYATARCRSTTVPSSTCMHYQPLDQTRQILSKGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YDY4</accession>
    <name>A0A9Q0YDY4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">RNA-binding protein 38</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_40776</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8021022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YDY4"/>
    <dbReference type="OrthoDB" id="4207594at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12384">
        <property type="entry name" value="RRM_RBM24_RBM38_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050886">
        <property type="entry name" value="RNA-binding_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48024">
        <property type="entry name" value="GEO13361P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48024:SF56">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN A0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="32"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="129"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="131"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8021022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="150" mass="16383" checksum="EB847A43843BEE49" modified="2023-09-13" version="1">MNYTSAMVPTSPTSTGVPEKKSPHVQKDTTFTKIFVGGLPFHTNDESLRAYFEPFGEIEEAVVITDRNHGRSKGYGFVTMATKEAAEQATSEPNPIIDGRKANVNLAYLGAKPRNNPTGPGIVEMRFLPQSARQTPSQAALLQTPYGRSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YF56</accession>
    <name>A0A9Q0YF56_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">E3 ubiquitin-protein ligase UHRF1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_39770</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YF56"/>
    <dbReference type="OrthoDB" id="2270193at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044027">
        <property type="term" value="P:negative regulation of gene expression via chromosomal CpG island methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.280.10">
        <property type="entry name" value="SRA-YDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036987">
        <property type="entry name" value="SRA-YDG_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003105">
        <property type="entry name" value="SRA_YDG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045134">
        <property type="entry name" value="UHRF1/2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14140">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE UHRF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14140:SF27">
        <property type="entry name" value="OS04G0289800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02182">
        <property type="entry name" value="SAD_SRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00466">
        <property type="entry name" value="SRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51015">
        <property type="entry name" value="YDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040265135" evidence="4">
        <location>
            <begin position="16"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="YDG" evidence="5">
        <location>
            <begin position="1"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="127"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="150"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8020235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="164" mass="18468" checksum="4FF49D0F5F1A00C4" modified="2023-09-13" version="1">MLEVCLCLLILSCHNLYIFICIGGRDLKGTKSNPKNLRTAPQSKDQTLTRGNLALSRSAETGKPVRVIRGYKLDSVYAPEDGYRYDGRWAHHKYQMGLYTVERYWFTTGLSGYGVYKFALKRCPDQAPPPWGDVTESQTALDQEEEDASSDSGFSEKNSDAIQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YH76</accession>
    <name>A0A9Q0YH76_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_40035</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YH76"/>
    <dbReference type="OrthoDB" id="70850at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38585">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38585:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8020444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="299" mass="32937" checksum="378082D90099BE6C" modified="2023-09-13" version="1">MSPRLPSNESSDDATTPLNCLRIVYDKWYHASTNLTQSDALRLSASWAVGAVTFPTCLGLLQKFVFAPLQISNSNLFANIFGMFAVAVSGVIASKVMGNAWNLAKDDESVVEAAETSFLESPETLFLVAGTSVSVFVLLRGKFSSVLPSDVLTPGAFGKVNLPASENYATTSKRLQLQVIGKKNGCHSCGSRHIQGEFIADHQPPLAIVKRKTSKTKHLLYRILKFFRKKTKYHPVQQKFYPQCNSCSNVQLTYLSAVSRGLKTPRHLVTHSLSLRLYHIFTPIPLLLASAYSFCTSED</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YJI9</accession>
    <name>A0A9Q0YJI9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_36223</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YJI9"/>
    <dbReference type="OrthoDB" id="10055806at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="115"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="49"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8023708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="208" mass="23441" checksum="83BB7EE347E20C23" modified="2023-09-13" version="1">MDATFDVTSTLMFNASKNEEEFVCRSRIDEVNITDEITLILVSYVLPEPILTVEDNFSDVIYIEQEEKVTITCDASGAKPQAVLIWMINERPVDKTNFKIRNEEGQPEDGRSDNLIIWVVLSLVATFMTVLVCCVGSATRRVKVLISPEIDNEEIYCDVEDKAEEVANVEMIVQLPGEGMFQYWTANATFNKDVRTKIIAKCVSGKVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YJP6</accession>
    <name>A0A9Q0YJP6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">branched-chain-amino-acid transaminase</fullName>
            <ecNumber evidence="3">2.6.1.42</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_36030</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.42" evidence="3"/>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YJP6"/>
    <dbReference type="OrthoDB" id="1732691at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004084">
        <property type="term" value="F:branched-chain-amino-acid transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:L-leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:L-valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01557">
        <property type="entry name" value="BCAT_beta_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.10.10:FF:000004">
        <property type="entry name" value="Branched-chain-amino-acid aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.10:FF:000002">
        <property type="entry name" value="Branched-chain-amino-acid aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.10.10">
        <property type="entry name" value="D-amino Acid Aminotransferase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001544">
        <property type="entry name" value="Aminotrans_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036038">
        <property type="entry name" value="Aminotransferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005786">
        <property type="entry name" value="B_amino_transII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043132">
        <property type="entry name" value="BCAT-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043131">
        <property type="entry name" value="BCAT-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033939">
        <property type="entry name" value="BCAT_family"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01123">
        <property type="entry name" value="ilvE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009897">
        <property type="entry name" value="PRK13357.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11825:SF44">
        <property type="entry name" value="BRANCHED-CHAIN-AMINO-ACID AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11825">
        <property type="entry name" value="SUBGROUP IIII AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01063">
        <property type="entry name" value="Aminotran_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006468">
        <property type="entry name" value="BCAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56752">
        <property type="entry name" value="D-aminoacid aminotransferase-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4 10" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="8" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="9">
        <location>
            <position position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR006468-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8023559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="414" mass="46565" checksum="D8C040A2E7C63432" modified="2023-09-13" version="1">MNMAFRLFFQRTCSAHSKLLLSSGQLAKNNLLIWRSFTSPASTFKYADLQVQKSTNLKEKPDPDTLIFGKNFTDHMLTIEWSSTEGWHAPKISPFANLSLHPASSVFHYAVELYEGLKAYRGVDGSVRVFRPYENMKRMLNTAARATLPLFDSAELVQCIAHLVNLDIDWVPPSTTSSLYIRPTFIGIEPSLGINNSNKALLFVILCPVGPYFKTGTFSPVSLYADTANVRAWKGGVGNFKLGSNYGPTIHPQKAAEKMGCQQILWLYGEDHYLTEVGTMNIFILWENENGEKELLSPPLDGTILPGITRKSLLELGEEWGEFKVSERPITMPQLATALRQNKVHEMFGAGTACVVCPVGSILYQGEQFEVPTMKDGGKLCSRFYQTLMDIQYGRMEHRWSYTVNDLLHGIRRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YKR9</accession>
    <name>A0A9Q0YKR9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">p-selectin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_40228</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YKR9"/>
    <dbReference type="OrthoDB" id="10014409at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051277">
        <property type="entry name" value="SEZ6_CSMD_C4BPB_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45656">
        <property type="entry name" value="PROTEIN CBR-CLEC-78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45656:SF4">
        <property type="entry name" value="PROTEIN CBR-CLEC-78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50948">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0768">Sushi</keyword>
    <feature type="domain" description="Sushi" evidence="5">
        <location>
            <begin position="109"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="5">
        <location>
            <begin position="245"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="5">
        <location>
            <begin position="314"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="5">
        <location>
            <begin position="382"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="6">
        <location>
            <begin position="444"/>
            <end position="514"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8020596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="611" mass="66809" checksum="79757B5A48C9CE1F" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YKU0</accession>
    <name>A0A9Q0YKU0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">26S proteasome regulatory subunit 8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_34301</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YKU0"/>
    <dbReference type="OrthoDB" id="1154031at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000502">
        <property type="term" value="C:proteasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19502">
        <property type="entry name" value="RecA-like_PAN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000006">
        <property type="entry name" value="26S protease regulatory subunit 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000044">
        <property type="entry name" value="26S protease regulatory subunit 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000030">
        <property type="entry name" value="26S protease regulatory subunit 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050221">
        <property type="entry name" value="26S_Proteasome_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032501">
        <property type="entry name" value="Prot_ATP_ID_OB_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23073">
        <property type="entry name" value="26S PROTEASOME REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16450">
        <property type="entry name" value="Prot_ATP_ID_OB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7 13" id="KW-0647">Proteasome</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="12">
        <location>
            <begin position="181"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="27"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00040909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8024400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="405" mass="45591" checksum="04A5E88EFB26EF30" modified="2023-09-13" version="1">MENGGVMEVDDGVHDGEGIKDYYVTKIEELQMVVTERELNLRRLEAQRNELNAKVRMLREELQLLQEQGSHVGEVVKAMDKKKVLVKVHPEGKFVVDIDKSINMTDITPNLRVALRNDSHTLHKILPNKVDPLVSLMMVEKVPDSTYEMIGGLDKQIKEIKEVIELPVKHPELFDALGISQPKGVLLYGPPGTGKTLLARAVAHHTECTFIRVSGSELVQKFIGEGARMVRELFVMAREHAPSIIFMDEIDSIGSTRIEGSSGGDSEVQRTMLELLNQLDGFEATKNIKVIMATNRIDILDSALLRPGRIDRKIEFPPPNEEARLDILKIHSRKMNLTRGINLRKIAELMPGASGAEVKGVCTEAGMYALRERRVHVTQEDFEMAVAKVMQKDSEKNMSIKKFWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YNS7</accession>
    <name>A0A9Q0YNS7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Solute carrier family 35 member G1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_34860</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YNS7"/>
    <dbReference type="OrthoDB" id="306876at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22911">
        <property type="entry name" value="ACYL-MALONYL CONDENSING ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22911:SF6">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER G1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="43"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="78"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="104"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="131"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="158"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="195"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="228"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="258"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="312"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="6">
        <location>
            <begin position="42"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="6">
        <location>
            <begin position="195"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8024836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="354" mass="38854" checksum="A846BDA0B83D2DD6" modified="2023-09-13" version="1">MAGEAVNCSNCDAEEVDQNKSDLVIATTTEVTRPSSPTAERKGLIYAFLCTCFLVGQTTSIAYLAGKVNSNEIISLRYFIMGTCCIVLNIYKGVPLLPSSKQEFLYLTFRSVLGVMAVTCAFYAFHHMRAADATAIHNAGPIVTLFFSCVFLKEGFECIDAILAILTVIGVVFIAKPPFLFPTERATQEKNSELFAVFIAIAGCVFLSLTHVYLRVKRDMKVLPSKLTMYYAYISLPLSVILTSALKHWTIPSCGLDRYLVVGIGLMSFIGECLYASSLVHVSAVYVSMMLTNEVMMVFLLETFLLNVPLSWLSIVGILLIVGASLTVSIKSTFPVKHLCSTVTRSSQSEEEQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YPA0</accession>
    <name>A0A9Q0YPA0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_33772</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8026038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YPA0"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="14"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8026038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="169" mass="18509" checksum="F956AAE4565D4A61" modified="2023-09-13" version="1">MARSVGSTTVWLDLVVPVAGFVGTWVGFLGTYDWIVRTMVGFGGTYSMARFIDTMVEFGGIYVLLGLLIHGMDLVVPMAGLFVLWLNVVVPAVWLGLLIPWLVLEKEVDKVNSTVTCVLKSSQGNVPKTFSRVELNTNLHQPPSNWLKSGTPIDARNTWVRGGSGYFSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YQ27</accession>
    <name>A0A9Q0YQ27_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Angiopoietin-related protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_31295</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YQ27"/>
    <dbReference type="OrthoDB" id="7841679at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050373">
        <property type="entry name" value="Fibrinogen_C-term_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143">
        <property type="entry name" value="FIBRINOGEN/TENASCIN/ANGIOPOEITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00186">
        <property type="entry name" value="FBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8026470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="194" mass="22201" checksum="9C823F01DF34B962" modified="2023-09-13" version="1">MFPFQILQRRPSGRVFGDRYWQDYKVGFGSTSNKHWLGNDKIFHLANQRNNRLRVDLEFSNGSKAYQEYSLFKIANESDNYRLIELGSSSGNAGDGMKGSLLQQFSTSDRDNDECDGIDCSAGHGNSGWWHSKEWCNECCHSEESTCFFQSSSKCNKRCTKANLNGGYNCSSLPWKIFWNGFHNIVSTEMKIRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YQ64</accession>
    <name>A0A9Q0YQ64_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein split ends</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_31378</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YQ64"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF1">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8026527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="276" mass="33448" checksum="9394EE1346E2ABD0" modified="2023-09-13" version="1">MPFNLDKFVASPSVEELDSLKKSEIVKVAKHYGIEFQPLMRKDEIKRYVLEYLVDESILPSTVLETAITVPTDNTFELKRLEMEMNKEIRLKEMEREREREEREREEREREREEREREMQMQKEKEEREMQMQKEKEKREMQMQREKEAREHEFRMKQLELGVIRGSDPKVGLGTGVFDISKHVKFVPKFQEDNVEKFFNHFEKLGEQLKWPRDKWSILIQSNFTGKAQEVYSALSIEDSMDYDKVKRPYNKPMNWCLKLIAKNLESIVRRIHKHM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YQF1</accession>
    <name>A0A9Q0YQF1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Homeobox protein EMX1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_33552</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YQF1"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000081">
        <property type="entry name" value="Empty spiracles homeobox 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050877">
        <property type="entry name" value="EMX-VAX-Noto_Homeobox_TFs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24339:SF28">
        <property type="entry name" value="E5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24339">
        <property type="entry name" value="HOMEOBOX PROTEIN EMX-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 5 6" id="KW-0371">Homeobox</keyword>
    <keyword evidence="4 5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="8">
        <location>
            <begin position="287"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="5">
        <location>
            <begin position="289"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="28"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="348"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="48"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="74"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8025871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="376" mass="41271" checksum="8594CF6250D5F70A" modified="2023-09-13" version="1">MQTKTPTSSPHRTESIKTFSKTSKFSIESLVSKEDPKGSQDIYPLADSPSSMNSSPIPSKSSSPEITYPHSDLSPRSRSPFAQSWHGQNSLSATKLNPVSEPVVLGSGAFQSLKRHRSERFTSSTIVPSTHSLAASRLGLSATPSGLGLPPHTLSTQSPHTFLTGLHHLQTAGHGAPFLALQSSQVHHTGAAAGMLGTLTAPGSGQFGREPFGSLYPWLLARNRMMATAHRFPATSTDIVFIRIMEKALLTTIDVLGGCKMDDTSRCFFGACSELAHNGFLFHSPFRKPKRIRTAFSPSQLLRLEQAFEKNHYVVGAERKQLAAGLNLTETQVKVWFQNRRTKYKRIKAEEDGEEEPKKKGSHHVTRWQIETQQAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YSC0</accession>
    <name>A0A9Q0YSC0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Octopamine receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_33219</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YSC0"/>
    <dbReference type="OrthoDB" id="10010417at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071880">
        <property type="term" value="P:adenylate cyclase-activating adrenergic receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043410">
        <property type="term" value="P:positive regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14967">
        <property type="entry name" value="7tmA_amine_R-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248">
        <property type="entry name" value="ADRENERGIC RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248:SF66">
        <property type="entry name" value="OCTOPAMINE RECEPTOR BETA-3R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="34"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="73"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="113"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="153"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="196"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="354"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="391"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="53"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="273"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="311"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="273"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8025617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="430" mass="47678" checksum="49D6C978F4D8D1D5" modified="2023-09-13" version="1">MSLHTNMENITVTTFNELLEEPTEFSSRASLRPIYRVFLAFLISIMCAFGIIGNCLVAFAIARYPSLRTVTNYLIVSLAVADTTVCAWVMPLAAYQDLNGGMWRLGDILCDLWVGSDVLMCTASIWNLCAVSVDRYLAISRPIWYATKRKPSFALILVFLAWSSSFMTSVMALFIVGGFQGNEYAEFCSVNTQPSFGVAASLLAFFIPACAILVLYTLILRSVMRLTISRPPLQTGRDQPSHSVELSTRYASTSKNLAKGTVGIANSSYGESDVPTTVTTTGDNKQTSGSVETVSSEVQASTMKYNEITAPAASDQGPSQPSVMMEPTKGTDEENRIKEKKKPVKISMTRERRAALVIGMVVLAFIACWLPFFSVFFMINVCTTCTVTLGIFQFVTWLGWFNSALNPVIYTVFNREFRHAFRKIFRFTDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YSZ7</accession>
    <name>A0A9Q0YSZ7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Dopamine receptor 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_33900</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8026142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YSZ7"/>
    <dbReference type="OrthoDB" id="5957871at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071880">
        <property type="term" value="P:adenylate cyclase-activating adrenergic receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043410">
        <property type="term" value="P:positive regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248">
        <property type="entry name" value="ADRENERGIC RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248:SF185">
        <property type="entry name" value="DOPAMINE RECEPTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="41"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="75"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="117"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="155"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="198"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="285"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="54"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8026142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="408" mass="46495" checksum="96AD613D49A4F7BB" modified="2023-09-13" version="1">MDPQNLGLQTVFQSNFTVINSTSNPLEDDIPTVWWVKCVQGFLTIVLSLGTVFGNFLVIFAVINERRLRLRVTNMFISSLACADFLIGAVVLPFAIYDLLAEGHWIFGRDWCDMWHAFDVLCCTASITNLCVIAIDRFWAITRPMSYQRHISKKVALAMIGIAWLSSSAISFPCILWWRAVEPVTLSTECIFPQDPFYLVLSSFISFYLPSIIMIVLYLQVYRTAVKQVRAIRTGTKKINGTKHVTGMTMRIHQGVKRDKGLNGQNKTRKQRTTRMNKEHKAARTLGIVMGVFIACWFPFFLVNVVAPLCPNCIPIPFVTFPTVTWLGYANSAINPIIYAFSSRTFQHAFKVILCRNRCFSLCRKKRNNFVSRSVTSTSMTWFDASDGKCSENESPTHRPYSLPFIYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YTK6</accession>
    <name>A0A9Q0YTK6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BEN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_32092</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YTK6"/>
    <dbReference type="OrthoDB" id="10679106at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2590">
        <property type="entry name" value="BEN domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018379">
        <property type="entry name" value="BEN_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10523">
        <property type="entry name" value="BEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01025">
        <property type="entry name" value="BEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51457">
        <property type="entry name" value="BEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BEN" evidence="3">
        <location>
            <begin position="279"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="76"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="181"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8027059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="401" mass="45928" checksum="CF14DEB22EF4920B" modified="2023-09-13" version="1">MDKIRVRWLEPKRAFGKTSFVFENDIICDKEKDLPAGCIVKAKWKSSDRIYKAILLAPLQAKCSQGKEISECKLNGSLDSSTEESPSAHFDRDTDLEDEEDYAQASVNANDTSSTSSHEDEIYETVTENNSEEPLQCEVSTVQYDMLQELKHVIKSEMDVMRCDFEKEIKEEMRREFDAFRKDMEMEMDSVKRKYEKENANLKKVVEKLLREVQDDVAQQIKLVLDAVSELNDIASSRDAPNHIVKHIRPAVEQSEQQEHSPVVLKDGKREQAAFGRVERGYVVAYEDDKKILSIKCDRYSKMFHGARTPTSFLRKLLRESFSSETLAKSNYNGGKVTCFNGVIKKKVLDRDRVHAIIEQVKLEFPGSASGMEADGKLRDAVNEECRQAARKLRLQEKYGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YU83</accession>
    <name>A0A9Q0YU83_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Adenosine receptor A2b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_31477</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YU83"/>
    <dbReference type="OrthoDB" id="5977652at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050569">
        <property type="entry name" value="TAAR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24249:SF372">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24249">
        <property type="entry name" value="HISTAMINE RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="51"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="90"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="132"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="178"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="230"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="31"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8026596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="317" mass="35892" checksum="623C008390049991" modified="2023-09-13" version="1">MLPSFQLGVSKYWLIFYIFVYSVIFTGSIVGNQLVIWSFIIESKLRRPTNFFIVSLAVTDFLTGLIAVPLGTVARLFTGTKVCLASVRSFYYIATFVFGSITICHLLLITIDRYIAVSQPLKYRNILTKRRCKILITIAWITGSLFGTLPYSSVPPEPNLWVCGVTNDRTAGVGIQKLTSAAIIPLTLLLLSLCYVRIFSIARKHVEETARLQNLDVDASTRLKMMQTTVTTAIILGVFSICWLPSTAKFFIEIYIETTDKQLFVIQTTAEVLVYGNSMMDPIIYGYRNDLFRLTYKRIFHRLCHCFQSNTTSGTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1B9B8</accession>
    <name>A0A9Q1B9B8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NACHT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_44524</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000964">
        <property type="protein sequence ID" value="KAJ8017825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1B9B8"/>
    <dbReference type="OrthoDB" id="120976at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="196"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="204"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="221"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="259"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8017825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="683" mass="78847" checksum="3B666DDE7EAB44F4" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1B9X6</accession>
    <name>A0A9Q1B9X6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_45007</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010001614">
        <property type="protein sequence ID" value="KAJ8017523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1B9X6"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8017523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="56" mass="6434" checksum="E447B074122BADCD" modified="2023-09-13" version="1">MVVKILLMKLSAISKQTSFSKIMKSRPLLTEFLFTSLCTSLNALRNSRSVHRKHKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BAQ6</accession>
    <name>A0A9Q1BAQ6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_41450</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8019745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BAQ6"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="142"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="52"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="170"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8019745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="190" mass="21038" checksum="89AFAB11E20C3E4E" modified="2023-09-13" version="1">MQGIRGLLHEFQIDCLNLVHRYRQRRQLFSSHSNNPHDLASHSSTSNLGSPSAAAVLASTSTATWQAPPQQQQQPQQQPPASPAITFNLDAAQSPQFFSQSSWTRTTEWEPGMPPAPSATLSRLLHQHLQCRRCQATFKTPVTLTQLPGPDPGVRRQQPKGISPQPSSPPRNSTHHQSNGIPRQRCQQGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BAR0</accession>
    <name>A0A9Q1BAR0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein NLRC5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_43484</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000327">
        <property type="protein sequence ID" value="KAJ8018498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BAR0"/>
    <dbReference type="OrthoDB" id="427518at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01120">
        <property type="entry name" value="RecA-like_superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.220.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000906">
        <property type="entry name" value="ZU5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312">
        <property type="entry name" value="NACHT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312:SF2">
        <property type="entry name" value="NUCLEOTIDE-BINDING OLIGOMERIZATION DOMAIN-CONTAINING PROTEIN 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00791">
        <property type="entry name" value="ZU5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51145">
        <property type="entry name" value="ZU5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NACHT" evidence="2">
        <location>
            <begin position="189"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="ZU5" evidence="3">
        <location>
            <begin position="936"/>
            <end position="1076"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="493"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="495"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="508"/>
            <end position="517"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8018498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1229" mass="141113" checksum="BFDD8BC6EC09D40E" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BAT6</accession>
    <name>A0A9Q1BAT6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Immunoglobulin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_44567</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000999">
        <property type="protein sequence ID" value="KAJ8017792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BAT6"/>
    <dbReference type="OrthoDB" id="5359219at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005911">
        <property type="term" value="C:cell-cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050839">
        <property type="term" value="F:cell adhesion molecule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051275">
        <property type="entry name" value="Cell_adhesion_signaling"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11640:SF164">
        <property type="entry name" value="MAM DOMAIN-CONTAINING GLYCOSYLPHOSPHATIDYLINOSITOL ANCHOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11640">
        <property type="entry name" value="NEPHRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Immunoglobulin" evidence="6">
        <location>
            <begin position="28"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="Immunoglobulin" evidence="6">
        <location>
            <begin position="247"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8017792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="345" mass="39042" checksum="7BC42B3E6BE59F9B" modified="2023-09-13" version="1">MKLFQLTAVQVFIYIINIRGFESNYCRSPQYIRTDSEEIIKCSFKDDVFSVLWYNSKDFYRVDAIVTLKDSVKSGEGFSSGEFDIFPNGSLIISNVSLKHEGTYTVIKLDTPTSRPVTYIINVFVIARTFERIPIIEGCDNGHVICFQTVGTTSKVSCTVTDARPAIPLSWMVRTTNGDRNMSSRSVITNETNVYFTSRTTILDPFAYSEILTLLACKADGPLGLLERNESLVLLQNSNETLINIKPKQIAVEIDSKVQLHCNYSKVSYLVWQVKQPSEAVFKAILYIVFVEENFTHINDKDYKLQQASLVIEKIRVQDEGTYRCVSGNGLEDNVIVYSVILFGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BBL1</accession>
    <name>A0A9Q1BBL1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Fibropellin-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_42482</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000079">
        <property type="protein sequence ID" value="KAJ8019129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BBL1"/>
    <dbReference type="OrthoDB" id="430340at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045197">
        <property type="term" value="P:establishment or maintenance of epithelial cell apical/basal polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007157">
        <property type="term" value="P:heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000039">
        <property type="entry name" value="Crumbs cell polarity complex component 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000123">
        <property type="entry name" value="Crumbs homolog 1 (Drosophila)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000004">
        <property type="entry name" value="Neurogenic locus notch 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000173">
        <property type="entry name" value="Neurogenic locus notch protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058912">
        <property type="entry name" value="HTH_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051022">
        <property type="entry name" value="Notch_Cell-Fate_Det"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24049">
        <property type="entry name" value="CRUMBS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24049:SF22">
        <property type="entry name" value="DROSOPHILA CRUMBS HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26215">
        <property type="entry name" value="HTH_animal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00010">
        <property type="entry name" value="EGFBLOOD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1 6" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="EGF-like" evidence="7">
        <location>
            <begin position="35"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="7">
        <location>
            <begin position="74"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="7">
        <location>
            <begin position="112"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="7">
        <location>
            <begin position="154"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="62"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="100"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="142"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8019129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="379" mass="42121" checksum="BE036659F9B561F0" modified="2023-09-13" version="1">MQVFGRMYAASLMGHPWAHHSTCNNSTILTVGGHDIDECASNPCLNNAVQCLDGPNFFFCVCAPGYTGIRCETEIDECASRPCQNGATCNDFVNMYNCTCPPNFEGVNCENEIDPCLSEPCLNNATCSELGGDTSIPFQCLCVDGFAGELCEEDIDECASMPCQNNATCNNLLNMYTCDCPGFEGVNCETVVFLDTELSISDRKIKSNLHFKPTDSHNYLMYPSNHPRSCTNSIPFSQLLRASRICSDDQDFAKVSKQIISFFEQRQYPQRVLSNALKRIQGIDRASALAPKTDHTPTRRIPLVLSFHPSVTPIVRAIYRNVETLRHDPSTRDHFFEPPITAFRIEKNISKHLVRASQPQAVVPNTPGTFPCNRGRCNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BC73</accession>
    <name>A0A9Q1BC73_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_41614</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8019849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BC73"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8019849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="70" mass="7321" checksum="E5D9E37F898A9013" modified="2023-09-13" version="1">MNQVAGSGGGGAGASNFRKHPKLKLKGKKANQSNFGPTHREGVVVIDLNEGASAPLAPLTLMDRDYSTTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BCG3</accession>
    <name>A0A9Q1BCG3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Beta,beta-carotene 9',10'-oxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_38732</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="5">Binds 1 Fe(2+) ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the carotenoid oxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BCG3"/>
    <dbReference type="OrthoDB" id="1069523at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003834">
        <property type="term" value="F:beta-carotene 15,15'-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010436">
        <property type="term" value="F:carotenoid dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016121">
        <property type="term" value="P:carotene catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042574">
        <property type="term" value="P:retinal metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004294">
        <property type="entry name" value="Carotenoid_Oase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10543">
        <property type="entry name" value="BETA-CAROTENE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10543:SF24">
        <property type="entry name" value="CAROTENOID ISOMEROOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03055">
        <property type="entry name" value="RPE65"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0408">Iron</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="5">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="320"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR604294-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8021500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="326" mass="36534" checksum="0C4E2C2868C86074" modified="2023-09-13" version="1">MQVGEDFYALTESASLMKVDPEVLETEDKVDLRNYVAVNVATAHPHYGDDQTVYNMGTSYGGQSFYNIIKIPPPNPGSTTDPLEKASVLCKIPASSSLYPSYFHSLAITKNYIVFLEQPLTINIIKILFARILGRNFAGCIDFTPSSLAKFHVVRLEDGAILPQKYTADAFFCFHHINAFEDNGHLVVDLCAYDDIQVIFNTYMEVLRRGNPPVTDAFAKRFVFPLVDGIDELAVHRFIRCDAFAPEVEGPRINYDKYNGQKYRYFYSLSDDVQRAGVVLSSVIDLKGTGRPPFLVVLDAKTFTELGRAEIPADVPFGFHGIFIQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BCG8</accession>
    <name>A0A9Q1BCG8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Carbohydrate sulfotransferase</fullName>
            <ecNumber evidence="9">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_37620</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Golgi apparatus membrane</location>
            <topology evidence="1 9">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the sulfotransferase 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="9"/>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BCG8"/>
    <dbReference type="OrthoDB" id="2019940at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016051">
        <property type="term" value="P:carbohydrate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018011">
        <property type="entry name" value="Carb_sulfotrans_8-10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005331">
        <property type="entry name" value="Sulfotransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12137">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12137:SF33">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03567">
        <property type="entry name" value="Sulfotransfer_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="8 9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8022656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="277" mass="33187" checksum="C0CA90AA8E3A2B59" modified="2023-09-13" version="1">MKELPEVLLNDANSQNWWNAMLKRQIERKLHLRRMCETRYNQSVLPLTEFIKDNKRLHSFYYVDKFKLIYSFVHKVGSSNWARMLKTVTENNRLKRLYLLPTDEALWRLQNYTSFIIVRHPLVRLLSAYKDKFVEHHIPHFKSIGRQIMKRYRDGVTMEELQRADNLRFGEFLRYLVADKRNRGNTHWDNLIIRNRVCLFNYDIIAKLETGDDDTKFILKSFNLEGLVNLGSHYTNYTGKKDTLRSFYSKIPKSLLQSVIDAYESDFVLFNYSKDVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BD50</accession>
    <name>A0A9Q1BD50_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Gametocyte-specific factor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_39876</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BD50"/>
    <dbReference type="OrthoDB" id="10069248at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022776">
        <property type="entry name" value="TRM13/UPF0224_CHHC_Znf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051591">
        <property type="entry name" value="UPF0224_FAM112_RNA_Proc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21402:SF5">
        <property type="entry name" value="GAMETOCYTE SPECIFIC FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21402">
        <property type="entry name" value="GAMETOCYTE SPECIFIC FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05253">
        <property type="entry name" value="zf-U11-48K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51800">
        <property type="entry name" value="ZF_CHHC_U11_48K"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CHHC U11-48K-type" evidence="5">
        <location>
            <begin position="28"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="CHHC U11-48K-type" evidence="5">
        <location>
            <begin position="60"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="121"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="159"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="173"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8020312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="288" mass="31944" checksum="0B405997CAA8F7C4" modified="2023-09-13" version="1">MGDKYQKPGPKGFGIIEASQQLVDKEQLFPCPYDPLHLVRAKRFQYHLMKCRKNYAGQDMVPCPFNARHVIPRPEQRHHFANCPDKALIEPDHEYHTVRKHNPDYQGNISVPKYLQDREVPNTSESWDDDIENDLGQYSYPPARNPSGSLSYQMAARSNGHERLHEEPPSLRQPRSQPASMGAPQYQQVPSGYNQSYPQVSEGTPQNLQPSADGQETGLPAAPSPTWSQIAGIGRGIPKRTGNFPVAGQSAPTGIGRGIVRPPPGFGRGQHGQRAGPLIAANIYNDVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BEC8</accession>
    <name>A0A9Q1BEC8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Monocarboxylate transporter 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_35988</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BEC8"/>
    <dbReference type="OrthoDB" id="410267at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008028">
        <property type="term" value="F:monocarboxylic acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF303">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="139"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="166"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="194"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="220"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="258"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="284"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8023525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="321" mass="35267" checksum="A0C9D5390F929DA0" modified="2023-09-13" version="1">MSFVEHFGESFPIMFSLREVSTYVGMSVMPPFMEVLQASYGLKGSYLVLGAIAWNCVICGLLLKPPQLNGKYRKSFCEELICLLCEVNCLQVESNMGSPSSEEGAGRLQILSRRFLRIFSLAPAIEHPRFAIFLIIHSFFYYTFTAWALFLVPFGISIGISKTVAVYLSTAGGIGGFVGKILAVAVFHFEKMNIISAGIIPSVICCIGLTGYILTTDYFLLLIFSSFCGFSLAFGDSALSAVVPEYLCQKHLKQGTSLSYTFGGIFMMLGGIVSGAILDILFSYVYLFGATIAFNIIIIILCAVLWCTRVSEPIEERTRPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BEJ5</accession>
    <name>A0A9Q1BEJ5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_34894</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BEJ5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="71"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="85"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="19"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="38"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8024861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="230" mass="25256" checksum="47AEB2A1760E1FB2" modified="2023-09-13" version="1">MGGQKKGANRNLLRAVTAGESTVQNTEQSVSAPSMIEALREGFKEQRSEFKQSSQREASNGKRRGKFSENCYMCWQPGHMARNCRDRDPLSPQNQQPRSRSKPNSKGHVCQHETWVGGIAGDGIIGHDFQKKFDGHISVEKGILSIKGQLVSCKKNGAGVLSCGVDNAENHVLPPRNEVITAVVRGNPAEQRLALVEMSDFFMNKHNVLVARALVDPSVGNVLLRAHECN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BER0</accession>
    <name>A0A9Q1BER0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_35154</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8025056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BER0"/>
    <dbReference type="OrthoDB" id="9988013at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040351310" evidence="2">
        <location>
            <begin position="22"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="140"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8025056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="162" mass="17680" checksum="0AFE01C46A822034" modified="2023-09-13" version="1">MTVKGLIYLAVCFSLLVRVSALRCYSASLQIDCTPRGEVCDKFSPSVNSSVTEIECTKEETMCALLTTKYTVESVKIESSHAWCLGKRLVANNLSGCYSGKDLKKAFPEIAKTLPEEEKDGIKIVNAELCICYGDLCNGAIKSFLSTLVIISSAIMNLILFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BF38</accession>
    <name>A0A9Q1BF38_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">UPF0686 protein C11orf1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_36967</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Microtubule inner protein (MIP) part of the dynein-decorated doublet microtubules (DMTs) in cilia axoneme, which is required for motile cilia beating.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Microtubule inner protein component of sperm flagellar doublet microtubules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Flagellum axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8024275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BF38"/>
    <dbReference type="OrthoDB" id="9970063at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030317">
        <property type="term" value="P:flagellated sperm motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009524">
        <property type="entry name" value="CFAP68"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037662">
        <property type="entry name" value="CFAP68/107"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31180">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 107-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31180:SF3">
        <property type="entry name" value="EXPRESSED SEQUENCE EH456644"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06608">
        <property type="entry name" value="CFAP68"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0969">Cilium</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0282">Flagellum</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8024275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="74" mass="8627" checksum="94121EED8151D0DD" modified="2023-09-13" version="1">MTDEVAFRSMIRASGLAEEWTHTTDEKKFQQYGWRTTTKEDCYRPNTLVGNWNEERFDISKVRVPDTLPSQVGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BFK8</accession>
    <name>A0A9Q1BFK8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Gamma-butyrobetaine dioxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_36297</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amine and polyamine biosynthesis; carnitine biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the gamma-BBH/TMLD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BFK8"/>
    <dbReference type="OrthoDB" id="406634at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008336">
        <property type="term" value="F:gamma-butyrobetaine dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045329">
        <property type="term" value="P:carnitine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00250">
        <property type="entry name" value="CAS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2020.30:FF:000002">
        <property type="entry name" value="Putative gamma-butyrobetaine dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.130.10:FF:000001">
        <property type="entry name" value="Trimethyllysine dioxygenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2020.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.130.10">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050411">
        <property type="entry name" value="AlphaKG_dependent_hydroxylases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010376">
        <property type="entry name" value="GBBH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038492">
        <property type="entry name" value="GBBH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042098">
        <property type="entry name" value="TauD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003819">
        <property type="entry name" value="TauD/TfdA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10696:SF33">
        <property type="entry name" value="GAMMA-BUTYROBETAINE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10696">
        <property type="entry name" value="GAMMA-BUTYROBETAINE HYDROXYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06155">
        <property type="entry name" value="GBBH-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02668">
        <property type="entry name" value="TauD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0124">Carnitine biosynthesis</keyword>
    <keyword evidence="7 12" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gamma-butyrobetaine hydroxylase-like N-terminal" evidence="11">
        <location>
            <begin position="22"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="TauD/TfdA-like" evidence="10">
        <location>
            <begin position="123"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF06155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8023763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="401" mass="46046" checksum="3F4BC6D04E1531FF" modified="2023-09-13" version="1">MGGTSEEKGSEKLVEVSGRNSQDAMYKLRWTDGHTSRYPFVWLRDNCRCTKCYHPSSYQRLALMADLDPEVKPTGEELSDEGSLLKVTWPDNHVGEYPSRWLRHHQFDKGFYDPIGDIKPKPWGSELGGNIPKYKYDEVLNDNDVLFEWLKTMYETGLAVLTGAPTKPGALEAIGKRVAYLRETVYGKKFQVYSKYSASSLAYTSAELGLHVDLPFYGFCPGVQMLHCVRQTKGSGGDNQFADAFHAALILKKEKPEVYKLLTTVDIHFRNCGTDHIDEYHLVKGRPMFEEDSEGNFKQVYYNDQVRASYLNVPVEKVQDVYRAIKLFHQTLYKNNISTKLRDGEVVTFNNTRVMHGRTGYTVDSSSGEQVRHYEGGYLEWDEIHSRMRILREKKEGELRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BGA8</accession>
    <name>A0A9Q1BGA8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Peroxin-7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_31485</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Peroxisome matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the WD repeat peroxin-7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BGA8"/>
    <dbReference type="OrthoDB" id="273771at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005053">
        <property type="term" value="F:peroxisome matrix targeting signal-2 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016558">
        <property type="term" value="P:protein import into peroxisome matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044536">
        <property type="entry name" value="PEX7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46027">
        <property type="entry name" value="PEROXISOMAL TARGETING SIGNAL 2 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46027:SF1">
        <property type="entry name" value="PEROXISOMAL TARGETING SIGNAL 2 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="5 11" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="104"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="148"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8026601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="322" mass="36170" checksum="0618708A40AA828E" modified="2023-09-13" version="1">MEFPKIAFRSPARHGYSVEYSPYAPNRLAVASSQNYGIAGAGTLYVVQVTRSGATLIKNFEWSNGLFDVTWAENNENVLLSVSGDGTIQLWNLMKEGSEPVMVFKEHTKEVYGIDWSQTRDQHLVLSASWDKTVKLWDPQRTTSMATYAAHNQIVYSAIWSPLVPGCFASAAGDGVCRIWDIRRPQAPRLAVTAGAEVVSCDWCKYDKNILVAGAVDGKIYGWDLRNVNMPAFILEQNGHTHAVRRVKCSPHHHSIIGSCSYDFTTRFWDFAGQPTPIETIQHHTEFVCGIDFNLHIQAEVADCAWDEKTIIFRPKCLQNIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BGG7</accession>
    <name>A0A9Q1BGG7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_36258</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BGG7"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8023732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="64" mass="7447" checksum="577B5B9CE343C2BD" modified="2023-09-13" version="1">MTKPAFRICIETLSIAHQTYMPLKKPVFCLLIREHLSTFAAVLLRELLSEDIDNKKTVVRFEAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BIL3</accession>
    <name>A0A9Q1BIL3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">UPF0769 protein C21orf59-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_32374</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CFAP298 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BIL3"/>
    <dbReference type="OrthoDB" id="276065at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003352">
        <property type="term" value="P:regulation of cilium movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021298">
        <property type="entry name" value="CFAP298"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13238:SF0">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 298"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13238">
        <property type="entry name" value="PROTEIN C21ORF59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11069">
        <property type="entry name" value="CFAP298"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8027272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="253" mass="28814" checksum="163FF8D6E3595001" modified="2023-09-13" version="1">MFLSHFLFLEISELADHGVSLPPNMQGLTDEQIVDLKLKDEWGEKCIPSGGAVECKDEVGRRNGKAPNEKMKEVLRKTMSEAKKIVSNDQVKANVCVTQEMVKEAIDQLRGAVTIVYPMGLPTYDPIRMEFEDREELGGTQASLQVLEEAATSLWFSGKELIPEKKMEAFVGKNEKTKLIVKLQKKGSGPPGREPVFSEEERKQMMLQAYRKQEEWKKLQEESEDSYLDSEWADSSALKKQFQGLKDVSWRPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BJG8</accession>
    <name>A0A9Q1BJG8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Macrophage mannose receptor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_29741</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8027711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BJG8"/>
    <dbReference type="OrthoDB" id="441660at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050111">
        <property type="entry name" value="C-type_lectin/snaclec_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013151">
        <property type="entry name" value="Immunoglobulin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22803">
        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00047">
        <property type="entry name" value="ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040323851" evidence="5">
        <location>
            <begin position="28"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="609"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="7">
        <location>
            <begin position="41"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="7">
        <location>
            <begin position="175"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="337"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="364"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="480"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="493"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="524"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="571"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="586"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="341"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="359"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8027711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="652" mass="72300" checksum="6194ECDFE5F02B57" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BKZ0</accession>
    <name>A0A9Q1BKZ0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Cathepsin O</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_30899</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BKZ0"/>
    <dbReference type="OrthoDB" id="498368at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02248">
        <property type="entry name" value="Peptidase_C1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039417">
        <property type="entry name" value="Peptidase_C1A_papain-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013201">
        <property type="entry name" value="Prot_inhib_I29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08246">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00705">
        <property type="entry name" value="PAPAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00848">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="domain" description="Cathepsin propeptide inhibitor" evidence="4">
        <location>
            <begin position="33"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="3">
        <location>
            <begin position="123"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8028608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="340" mass="38230" checksum="F95C2EAEA46814AF" modified="2023-09-13" version="1">MGTYSSLWLYYVTVIITFSVIGLSSPASVEELFEDFVHKYKKSYVRGSSEYRQRLSNFEKSLQRHKTLNQLSSHPQDAIYGVTKFSDLTPEEFYEQILGYSQGVNNSQIHLKSSILLVSLPKGPKSFDLRKQKWPVITPVKDQKKCGGCWAFSITETLETQYALKHKVLYTLSEQQLIDCTVGEGNDGCKGGDIVRTARWLNNTKTKITTEKKYPFIDKTGSCHKLKKSDGKVGITDYSYQSFINEEFQMADLVASHGPLTVAVDAVSWQDYLGGIIQHHCYAHDDNHAVQIIGYDKTGPVPYFIVRNSWGKNWGLNGYLHVKIGGNLCGIANSVVTVSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BPN9</accession>
    <name>A0A9Q1BPN9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Centrobin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_26655</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BPN9"/>
    <dbReference type="OrthoDB" id="8190486at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007099">
        <property type="term" value="P:centriole replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051299">
        <property type="term" value="P:centrosome separation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902410">
        <property type="term" value="P:mitotic cytokinetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902017">
        <property type="term" value="P:regulation of cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038923">
        <property type="entry name" value="Centrobin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34439">
        <property type="entry name" value="CENTROBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34439:SF1">
        <property type="entry name" value="CENTROBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="73"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="712"/>
            <end position="732"/>
        </location>
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        <location>
            <begin position="853"/>
            <end position="899"/>
        </location>
    </feature>
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            <begin position="1077"/>
            <end position="1142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1154"/>
            <end position="1224"/>
        </location>
    </feature>
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        <location>
            <begin position="328"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="395"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="537"/>
            <end position="630"/>
        </location>
    </feature>
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        <location>
            <begin position="85"/>
            <end position="99"/>
        </location>
    </feature>
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        <location>
            <begin position="101"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="141"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="853"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1090"/>
            <end position="1103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1110"/>
            <end position="1136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1181"/>
            <end position="1197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8030289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1224" mass="138813" checksum="85F5C9721358EDEF" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BQ60</accession>
    <name>A0A9Q1BQ60_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Neutral cholesterol ester hydrolase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_27049</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BQ60"/>
    <dbReference type="OrthoDB" id="408631at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050300">
        <property type="entry name" value="GDXG_lipolytic_enzyme"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081">
        <property type="entry name" value="AB HYDROLASE SUPERFAMILY PROTEIN C4A8.06C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081:SF8">
        <property type="entry name" value="ALPHA_BETA HYDROLASE FOLD-3 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040220145" evidence="2">
        <location>
            <begin position="17"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="3">
        <location>
            <begin position="114"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="3">
        <location>
            <begin position="337"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8030591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="424" mass="47726" checksum="EA2F1AF310F381D0" modified="2023-09-13" version="1">MGKFLIFSVLATAFLAYLLNPAVPGGLEERFKYRSFFAFFNACHLWAELSSMLSSGDRSNNVVMVTNNAICLLADHYPTDHFGPGVITTEGVFDGTRVLIYRPPMTSDQLLPGFVYFHGGGLALATANFSPLFLTTETFDGLAKKIAYHVNAVVVNVDYDTTPNNKFPGPVNQAFAAVKWFMTHAEEYGVDPNRIAVGGDSAGGYLSSVTSYLVREDDSIPNLKLQILIYPWTQCLDLNFPSYQKYTMDFGPNEGIVCRPIMAEFCSLHAFGSARQELVGKFIENKHVSPSFRKSALYRKTFGHELLPAAYRDNSYYNGASQPDEGDEDFWNESKQIFLDPRFTPHFIKNMTGLPTAYVMTAGYDSLRDEGYLYAQQLKKAGVDVTLVHYERAWHGVHWVSPEFVFRLGEKIHNDFLNFVKERL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BQC0</accession>
    <name>A0A9Q1BQC0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Carbamoyl-phosphate synthase [ammonia], mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_27216</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BQC0"/>
    <dbReference type="OrthoDB" id="434at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004088">
        <property type="term" value="F:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1030.10">
        <property type="entry name" value="Carbamoyl-phosphate synthetase, large subunit oligomerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036897">
        <property type="entry name" value="CarbamoylP_synth_lsu_oligo_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005480">
        <property type="entry name" value="CPSase_lsu_oligo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11405:SF53">
        <property type="entry name" value="CARBAMOYL-PHOSPHATE SYNTHASE [AMMONIA], MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11405">
        <property type="entry name" value="CARBAMOYLTRANSFERASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02787">
        <property type="entry name" value="CPSase_L_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01096">
        <property type="entry name" value="CPSase_L_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48108">
        <property type="entry name" value="Carbamoyl phosphate synthetase, large subunit connection domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carbamoyl-phosphate synthetase large subunit oligomerisation" evidence="4">
        <location>
            <begin position="65"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8030729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="122" mass="13769" checksum="3B3D5A2A927815D8" modified="2023-09-13" version="1">MQGQTALNCGVELFKQGILQKHGVRVMAIGPTFEESLQRALRMIHPSFKGFAPEMPVGKATPKNLEAAMEVPSSNRIYAIAKALEDGYSVDQIHQLTSIDKWFLHKLQYIMGLMKDMKSFTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BT68</accession>
    <name>A0A9Q1BT68_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Retrotransposon gag domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_25878</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BT68"/>
    <dbReference type="OrthoDB" id="10070970at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8032366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="198" mass="22212" checksum="2AD922FA19AA85AB" modified="2023-09-13" version="1">MNTPENATPAPSLRQTDFLPARFSGNELNDDECLAHFLAFEEYLEAHKVNTGDPNNFSNLINTFQRTLGGTARICLEGKNFTVSELKRSFLARFSKHQSYICHVQSFNNTSYVKNESADQHLSKIERAASKLGYGDSQIRDKLLTSLPQECRASILMSLPHDVPTKDIVTKAQCYFDLHSTTNTTPEVTFNIADNSTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BUN1</accession>
    <name>A0A9Q1BUN1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Fibrinogen-like protein A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_23296</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BUN1"/>
    <dbReference type="OrthoDB" id="6275059at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050373">
        <property type="entry name" value="Fibrinogen_C-term_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143">
        <property type="entry name" value="FIBRINOGEN/TENASCIN/ANGIOPOEITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00186">
        <property type="entry name" value="FBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040319011" evidence="1">
        <location>
            <begin position="23"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="2">
        <location>
            <begin position="8"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8033146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="250" mass="28409" checksum="31FA5C13CB07A424" modified="2023-09-13" version="1">MELKVLNHFVVQFSLICFLVKGSILDQVHLTSSTNDLGIREIVKKSSSNVQLRIQVECISYNLMVFWSHSLFIAKVIQRRANNFIDFNRNWTEYSNGFGFLNSDFWVGNEKLAYLTNQKQYELNISFETEKEESYHVTYSMFRISDSFSEFTLVSVGEFTGTLGDLPTRPPTDCYDIQQAGNTTSGDYIVNPTGWSGGPFTINCNMTIDGEDGRYSKDVSTAPYHLIVPGRAIRRDSGIHPENSGWETTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BVB4</accession>
    <name>A0A9Q1BVB4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_23499</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IL-17 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BVB4"/>
    <dbReference type="OrthoDB" id="6038945at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010345">
        <property type="entry name" value="IL-17_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06083">
        <property type="entry name" value="IL17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8033305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="139" mass="16002" checksum="9A44159D8B5B89BB" modified="2023-09-13" version="1">MPNRRYAMSSTRKVGRLDVMDLANSFSNKVNEVDEWLRNQTRNCYENALDKACPWREVEEHDDNRIPRTVIKAVCVCEQGCINFGSNLMDSSLGFCEEVVKPTMVFRRQSANRPHIIDIELIPYACICKRNIAETTTAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BVL3</accession>
    <name>A0A9Q1BVL3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">E3 ubiquitin-protein ligase TRIM56</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_23582</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BVL3"/>
    <dbReference type="OrthoDB" id="10250935at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19757">
        <property type="entry name" value="Bbox1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047153">
        <property type="entry name" value="TRIM45/56/19-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25462">
        <property type="entry name" value="BONUS, ISOFORM C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25462:SF296">
        <property type="entry name" value="MEIOTIC P26, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117289">
        <property type="entry name" value="Nucleoporin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="16"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="6">
        <location>
            <begin position="95"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="6">
        <location>
            <begin position="156"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8033366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="694" mass="79661" checksum="48AD50780D1C54C8" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BVW4</accession>
    <name>A0A9Q1BVW4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_24046</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10000497at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 297-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon gypsy-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054465">
        <property type="entry name" value="Integrase_p58-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF13">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22938">
        <property type="entry name" value="Integrase_p58_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="3">
        <location>
            <begin position="838"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="307"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="571"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="671"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="78"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="307"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="322"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="346"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="571"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="595"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="615"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="640"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="687"/>
            <end position="711"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8033712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1285" mass="146403" checksum="03F89551A8CCDC45" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BW18</accession>
    <name>A0A9Q1BW18_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNA-directed DNA polymerase from mobile element jockey</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_24005</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BW18"/>
    <dbReference type="OrthoDB" id="416454at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33395:SF22">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33395">
        <property type="entry name" value="TRANSCRIPTASE, PUTATIVE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8033680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="114" mass="12500" checksum="353FC6D5E96DAE07" modified="2023-09-13" version="1">MHFRNDYLVSVFTKKSSILGTCRGSDDSSLVSDVVFSEELVCDKLKSLKASKAPGPDGIHPAILHECSKILSVPLCIIFQKFLDSNFVPADWRIANVTPIFKRRIGACLATIGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BYW7</accession>
    <name>A0A9Q1BYW7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_22207</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BYW7"/>
    <dbReference type="OrthoDB" id="6159398at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040194463" description="Ig-like domain-containing protein" evidence="2">
        <location>
            <begin position="24"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="233"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="33"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="128"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8035094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="272" mass="30732" checksum="F6DFB54DCBF27672" modified="2023-09-13" version="1">MPGKHRCIILIIIINTLGTLTSCQKWPILLFKEADSGVIPCIPRGQTTTFFWTRGEWYNISDEIAYNVNGVSGPDTEKFHVFSNGSLLLKNMTLMDEGNYYCRITSDRTECHGSVTVYLKATNTNKLPYIDRCSPNKGCTIYEKPSSTSYLTCKANQVSSDMSLKWFNESKEITEEVEKRQDKNTMNETIISSRIKINYDEPQFLTCQASSMKMETAFAHVRLQSEPVDGTSAGIIVFTVVLGATCAILLGFLVYTFVKQNRDKKTRSDSER</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BZ11</accession>
    <name>A0A9Q1BZ11_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_22259</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BZ11"/>
    <dbReference type="OrthoDB" id="416119at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8035134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="201" mass="23156" checksum="1C040FC3137ABAFA" modified="2023-09-13" version="1">MPFAYFVRYWGGLSFRWFFPTLFSNSVPHSWRPTKAYCQMRETLRIMSTVGDVLQSSAGDQYRAIFDDLVGQVQPNDYASGPELWRVVHSGTLDHRRRDLLWRIAHDAIVTNLRRLRWRLGDGICPRTGCTGWESVPHVFWQCGFVALLWEWFQALADRLTFCNTWAVSQDYALYGLSPPPCNTSVRGILTFVSASIKLAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BZM4</accession>
    <name>A0A9Q1BZM4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Fibrinogen-like protein A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_19407</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8035662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BZM4"/>
    <dbReference type="OrthoDB" id="10391232at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00087">
        <property type="entry name" value="FReD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19941">
        <property type="entry name" value="TIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050373">
        <property type="entry name" value="Fibrinogen_C-term_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036084">
        <property type="entry name" value="Ser_inhib-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025615">
        <property type="entry name" value="TILa_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040941">
        <property type="entry name" value="GGGWT_bact"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143">
        <property type="entry name" value="FIBRINOGEN/TENASCIN/ANGIOPOEITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12714">
        <property type="entry name" value="TILa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00186">
        <property type="entry name" value="FBG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57567">
        <property type="entry name" value="Serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040155144" evidence="1">
        <location>
            <begin position="25"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="2">
        <location>
            <begin position="34"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="2">
        <location>
            <begin position="298"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8035662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="509" mass="57489" checksum="080A840AE5CB6159" modified="2023-09-13" version="1">MRYLILLQMFSIFCSSLLVRKVYSSTEGSSYFFYQQSTYPRDCQEIFQQCSTNNASGVYLIKPDGYDEAFEVYCDNKVDGGGWTVFERRSVENSVEFRRNFSEYRQGFGFLSGEFWLGNEKLSFLTNQKRYQLRIDLKNSAGSSFYVTYDLFRISDDWGNYKLTSVGQYDGTADTFIGQCPANTIFGACACQGTCADPDGCHKTCTVEETCTCPAGYLLSGSDCVPQEQCSCFNPAFGVIPENESKFSSDCSEHCTCDEGTLMCDRNLRCDTNAVCEERNNLRKCYCNEGYAGDGQSCTRTGPYTDCMEVSTSRLPDGVYTIQPTGWTEAPFEVYCNMSHGGGWTVFQRRVDGSVSFDRNWAVYKAGFGSAGPNENVWLGNKILSFMTNQRNYKLRIDVTKQDGVPWHMNYDFFRVKSESNNYRLELGTYTGNAGYDYMSDHRGQDFSTPERDNDAYSYNCAAHSATRGGWWYKSCWTPTLIVEPTGLSILNCTIGPLILITITFSTPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C030</accession>
    <name>A0A9Q1C030_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Phosphoserine aminotransferase</fullName>
            <ecNumber evidence="12">2.6.1.52</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_19469</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>4-(phosphooxy)-L-threonine + 2-oxoglutarate = (R)-3-hydroxy-2-oxo-4-phosphooxybutanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:16573"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58452"/>
            <dbReference type="ChEBI" id="CHEBI:58538"/>
            <dbReference type="EC" id="2.6.1.52"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 12">
            <text>O-phospho-L-serine + 2-oxoglutarate = 3-phosphooxypyruvate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:14329"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:18110"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57524"/>
            <dbReference type="EC" id="2.6.1.52"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. SerC subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.52" evidence="12"/>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8035709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C030"/>
    <dbReference type="OrthoDB" id="1703350at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004648">
        <property type="term" value="F:O-phospho-L-serine:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006564">
        <property type="term" value="P:L-serine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00611">
        <property type="entry name" value="PSAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000010">
        <property type="entry name" value="Phosphoserine aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000006">
        <property type="entry name" value="Phosphoserine aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00160">
        <property type="entry name" value="SerC_aminotrans_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020578">
        <property type="entry name" value="Aminotrans_V_PyrdxlP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022278">
        <property type="entry name" value="Pser_aminoTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003764">
        <property type="entry name" value="PRK05355.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01364">
        <property type="entry name" value="serC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43247">
        <property type="entry name" value="PHOSPHOSERINE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43247:SF1">
        <property type="entry name" value="PHOSPHOSERINE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000525">
        <property type="entry name" value="SerC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00595">
        <property type="entry name" value="AA_TRANSFER_CLASS_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4 12 14" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0718">Serine biosynthesis</keyword>
    <keyword evidence="6 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class V" evidence="13">
        <location>
            <begin position="10"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ8035709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="370" mass="41487" checksum="54C4DFB8CB8413AE" modified="2023-09-13" version="1">MEAKRAKRVINFSPGPAKLPDWVLEKAQRELMNYNDLGISVMEMSHRSEDFKNILESAKRRLKTILKVPDNYQILFLQGGGTGQFSAIPLNLMVNGKADYVVTGGWSAKAAKEAEKYGKVNLVLPKLDQYNRIPEKVEWNLSEDADYVYYCANETVFGVEFPYIPDVKGVTLVCDMSSNFLTRNVDVAKYGVIFAGAQKNVGCAGVTVVIVRDDLLGKALKTCPSIIDFTVQAKNNSLYNTPPCYSIYILGLYMEWLENEGGVSALDKRNREKSSLIYKIIDESDGFYCCPVENSVRSRINIPFRIGDASGDETLEKKFLSESKEKGMVSLKGHRSVGGIRASLYNAMSLDETQVLAGFMRNFREKYDKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C1J8</accession>
    <name>A0A9Q1C1J8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 16">Guanylate cyclase</fullName>
            <ecNumber evidence="3 16">4.6.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">HOLleu_20258</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="22">Nanhai2018</strain>
            <tissue evidence="22">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 16">
            <text>GTP = 3',5'-cyclic GMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13665"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57746"/>
            <dbReference type="EC" id="4.6.1.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.2" evidence="3 16"/>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
        <property type="term" value="F:adenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004383">
        <property type="term" value="F:guanylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016941">
        <property type="term" value="F:natriuretic peptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017046">
        <property type="term" value="F:peptide hormone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007168">
        <property type="term" value="P:receptor guanylyl cyclase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1230:FF:000030">
        <property type="entry name" value="Si:ch211-215j19.12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018297">
        <property type="entry name" value="A/G_cyclase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001170">
        <property type="entry name" value="ANPR/GUC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050401">
        <property type="entry name" value="Cyclic_nucleotide_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11920:SF494">
        <property type="entry name" value="ATRIAL NATRIURETIC PEPTIDE RECEPTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11920">
        <property type="entry name" value="GUANYLYL CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00255">
        <property type="entry name" value="NATPEPTIDER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00452">
        <property type="entry name" value="GUANYLATE_CYCLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14 16" id="KW-0141">cGMP biosynthesis</keyword>
    <keyword evidence="17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="13 15" id="KW-0456">Lyase</keyword>
    <keyword evidence="10 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11 22" id="KW-0675">Receptor</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 19" id="KW-0732">Signal</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040322993" description="Guanylate cyclase" evidence="19">
        <location>
            <begin position="18"/>
            <end position="1471"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="904"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="20">
        <location>
            <begin position="949"/>
            <end position="1209"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="21">
        <location>
            <begin position="1255"/>
            <end position="1391"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="1221"/>
            <end position="1248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU003431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KAJ8036321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1471" mass="166404" checksum="659744C5E1300FC9" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C2Q6</accession>
    <name>A0A9Q1C2Q6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">aldehyde dehydrogenase (NAD(+))</fullName>
            <ecNumber evidence="4">1.2.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_17911</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.3" evidence="4"/>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="310895at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004029">
        <property type="term" value="F:aldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07141">
        <property type="entry name" value="ALDH_F1AB_F2_RALDH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000029">
        <property type="entry name" value="Aldehyde dehydrogenase, mitochondrial"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000026">
        <property type="entry name" value="Aldehyde dehydrogenase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000001">
        <property type="entry name" value="Mitochondrial aldehyde dehydrogenase 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1820">
        <property type="entry name" value="L1 transposable element, RRM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11699">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="2 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="8">
        <location>
            <begin position="54"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="8">
        <location>
            <begin position="871"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="8">
        <location>
            <begin position="946"/>
            <end position="1123"/>
        </location>
    </feature>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="8">
        <location>
            <begin position="1220"/>
            <end position="1591"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="631"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="639"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="293"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="1046"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="1459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8037163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1600" mass="176081" checksum="613C816AD3B010BE" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C6B1</accession>
    <name>A0A9Q1C6B1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Growth/differentiation factor 8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_14318</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the TGF-beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C6B1"/>
    <dbReference type="OrthoDB" id="5948587at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001839">
        <property type="entry name" value="TGF-b_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015615">
        <property type="entry name" value="TGF-beta-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848">
        <property type="entry name" value="TGF-BETA FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848:SF302">
        <property type="entry name" value="TGF-BETA FAMILY PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00019">
        <property type="entry name" value="TGF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00204">
        <property type="entry name" value="TGFB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51362">
        <property type="entry name" value="TGF_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0339">Growth factor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="TGF-beta family profile" evidence="5">
        <location>
            <begin position="151"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8040113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="269" mass="30463" checksum="6CFA9B1F575ED7CE" modified="2023-09-13" version="1">MKCLSRKKKKIKCRHQSFSLGGKVSEGCAETPQSACYRFQKEGKTECDIKSEKLWIFVKNSNSSGNDLLVFENHTNGSVQPIAKLNGTVSGWVPIELHPLHWTGQLEDKSRTFAFSYDHSSADVINQLVGIFKHHTPLIEFTMDECSTHDRETRSVRRQRCGRTRDPQCCLQEFTVNFTAIGWNWILLPRTVSANYCQGTCNLHILSQLEKNHRAMMYHSSGFYGNPNMLPCCVAVEKSSLHVIVYNPRDNTAVPRRVPGLSASGCECY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C7L2</accession>
    <name>A0A9Q1C7L2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Estradiol 17-beta-dehydrogenase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_13658</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8039609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C7L2"/>
    <dbReference type="OrthoDB" id="191139at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43391">
        <property type="entry name" value="RETINOL DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43391:SF86">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE_REDUCTASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8039609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="301" mass="33305" checksum="30F3274B8AE42CD2" modified="2023-09-13" version="1">MTSPSIKIVLITGTATGVGFTTALTFAGVPGKKYKVYATMRNMKTRKDELESAAGSMLNDTLFIRELDVGDEKSRVECVESILKEEKRIDILINNAAIVHGGLFEQIDLGKDGPKHFQINYFGPLRLMQLVIPSMKERKSGRILNISTIGTLLPCPFTDLYLHPKWALEGATECVAVTLRPFNVWASVVQLGAVMTQRAVQVQAASDQYKDAFLKPPNIKNETDQEIFAKHTKNSMSLFTHETCQTPEEVAKFLITVTEEEKPVLYYQSSEFARKLAAAKLVDPTGEKGAQLYEAGVLSIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C7M5</accession>
    <name>A0A9Q1C7M5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_14413</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C7M5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006970">
        <property type="entry name" value="PT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04886">
        <property type="entry name" value="PT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="11"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="30"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8040187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="57" mass="6290" checksum="4D30764D8241CB29" modified="2023-09-13" version="1">MYLRNDPTPVKINQSINQPAKQPSSQAAKQPTNQPTNQPTNQSINQSINQSINQSIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CAB1</accession>
    <name>A0A9Q1CAB1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_15479</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
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        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="KAJ8041001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
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        <source>
            <dbReference type="EMBL" id="KAJ8041001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="1">RNA helicase</fullName>
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        <name evidence="18" type="ORF">HOLleu_12699</name>
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        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Nanhai2018</strain>
            <tissue evidence="18">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome 5"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042078">
        <property type="term" value="P:germ-line stem cell division"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="ARM-like"/>
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    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
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    <dbReference type="InterPro" id="IPR007052">
        <property type="entry name" value="CS_dom"/>
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    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
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    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035437">
        <property type="entry name" value="SNase_OB-fold_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002999">
        <property type="entry name" value="Tudor"/>
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    <dbReference type="PANTHER" id="PTHR22655">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE TDRD12-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22655:SF2">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE TDRD12-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04969">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00567">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="2"/>
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        <property type="match status" value="2"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Staphylococcal nuclease"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5" id="KW-0221">Differentiation</keyword>
    <keyword evidence="7 18" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0469">Meiosis</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0943">RNA-mediated gene silencing</keyword>
    <keyword evidence="9" id="KW-0744">Spermatogenesis</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="15">
        <location>
            <begin position="638"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="16">
        <location>
            <begin position="852"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="domain" description="Tudor" evidence="14">
        <location>
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            <end position="1436"/>
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        <location>
            <begin position="1893"/>
            <end position="1980"/>
        </location>
    </feature>
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        <location>
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        <location>
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            <end position="1850"/>
        </location>
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        <location>
            <begin position="2007"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
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            <end position="232"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
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        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
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        </location>
    </feature>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50304"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ8041792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="2080" mass="232392" checksum="77784E5E751BB7A5" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CBC9</accession>
    <name>A0A9Q1CBC9_HOLLE</name>
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            <fullName evidence="5">15-hydroxyprostaglandin dehydrogenase [NAD(+)]</fullName>
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            <ecNumber evidence="4">1.1.1.232</ecNumber>
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        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Prostaglandin dehydrogenase 1</fullName>
        </alternativeName>
    </protein>
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        <name evidence="24" type="ORF">HOLleu_12488</name>
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        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="24">Nanhai2018</strain>
            <tissue evidence="24">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the NAD-dependent dehydrogenation (oxidation) of a broad array of hydroxylated polyunsaturated fatty acids (mainly eicosanoids and docosanoids, including prostaglandins, lipoxins and resolvins), yielding their corresponding keto (oxo) metabolites. Decreases the levels of the pro-proliferative prostaglandins such as prostaglandin E2 (whose activity is increased in cancer because of an increase in the expression of cyclooxygenase 2) and generates oxo-fatty acid products that can profoundly influence cell function by abrogating pro-inflammatory cytokine expression. Converts resolvins E1, D1 and D2 to their oxo products, which represents a mode of resolvin inactivation. Resolvin E1 plays important roles during the resolution phase of acute inflammation, while resolvins D1 and D2 have a unique role in obesity-induced adipose inflammation.</text>
    </comment>
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        <reaction evidence="13">
            <text>(11R)-hydroxy-(5Z,8Z,12E,14Z)-eicosatetraenoate + NAD(+) = 11-oxo-(5Z,8Z,12E,14Z)-eicosatetraenoate + NADH + H(+)</text>
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            <dbReference type="ChEBI" id="CHEBI:15378"/>
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            <dbReference type="ChEBI" id="CHEBI:78836"/>
            <dbReference type="ChEBI" id="CHEBI:90697"/>
        </reaction>
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            <dbReference type="Rhea" id="RHEA:48641"/>
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    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>(15S)-hydroxy-(5Z,8Z,11Z,13E)-eicosatetraenoate + NAD(+) = 15-oxo-(5Z,8Z,11Z,13E)-eicosatetraenoate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23260"/>
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            <dbReference type="ChEBI" id="CHEBI:57409"/>
            <dbReference type="ChEBI" id="CHEBI:57410"/>
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            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.232"/>
        </reaction>
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            <dbReference type="Rhea" id="RHEA:23261"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>14-hydroxy-(4Z,7Z,10Z,12E,16Z,19Z)-docosahexaenoate + NAD(+) = 14-oxo-(4Z,7Z,10Z,12E,16Z,19Z)-docosahexaenoate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48952"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:90866"/>
            <dbReference type="ChEBI" id="CHEBI:90867"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:48953"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>15-oxo-(5S,6R)-dihydroxy-(7E,9E,11Z)-eicosatrienoate + NADH + H(+) = (5S,6R,15S)-trihydroxy-(7E,9E,11Z)-eicosatrienoate + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:41596"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:78325"/>
            <dbReference type="ChEBI" id="CHEBI:78329"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:41597"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>lipoxin A4 + NAD(+) = 15-oxo-(5S,6R)-dihydroxy-(7E,9E,11Z,13E)-eicosatetraenoate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41572"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:67026"/>
            <dbReference type="ChEBI" id="CHEBI:78311"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:41573"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>prostaglandin A1 + NAD(+) = 15-oxo-prostaglandin A1 + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41263"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57398"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:85072"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:41264"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>prostaglandin E1 + NAD(+) = 15-oxoprostaglandin E1 + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16477"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57397"/>
            <dbReference type="ChEBI" id="CHEBI:57401"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:16478"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>prostaglandin E2 + NAD(+) = 15-oxoprostaglandin E2 + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11876"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57400"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:606564"/>
            <dbReference type="EC" id="1.1.1.141"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:11877"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>resolvin D1 + NAD(+) = 17-oxoresolvin D1 + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50128"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132079"/>
            <dbReference type="ChEBI" id="CHEBI:132081"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:50129"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>resolvin D1 + NAD(+) = 8-oxoresolvin D1 + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50124"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132079"/>
            <dbReference type="ChEBI" id="CHEBI:132080"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:50125"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>resolvin D2 + NAD(+) = 16-oxoresolvin D2 + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53588"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:133367"/>
            <dbReference type="ChEBI" id="CHEBI:137498"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:53589"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>resolvin D2 + NAD(+) = 7-oxoresolvin D2 + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53584"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:133367"/>
            <dbReference type="ChEBI" id="CHEBI:137497"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:53585"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>resolvin E1 + NAD(+) = 18-oxo-resolvin E1 + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:49244"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:91000"/>
            <dbReference type="ChEBI" id="CHEBI:91001"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:49245"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 22">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.141" evidence="3"/>
    <dbReference type="EC" id="1.1.1.232" evidence="4"/>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CBC9"/>
    <dbReference type="OrthoDB" id="37659at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047034">
        <property type="term" value="F:15-hydroxyicosatetraenoate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016404">
        <property type="term" value="F:15-hydroxyprostaglandin dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd05323">
        <property type="entry name" value="ADH_SDR_c_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44229">
        <property type="entry name" value="15-HYDROXYPROSTAGLANDIN DEHYDROGENASE [NAD(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44229:SF4">
        <property type="entry name" value="15-HYDROXYPROSTAGLANDIN DEHYDROGENASE [NAD(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="23" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="23">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040362588" description="15-hydroxyprostaglandin dehydrogenase [NAD(+)]" evidence="23">
        <location>
            <begin position="20"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00039060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00040276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00041812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00042026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00045705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00049151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00049188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="KAJ8041618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="289" mass="31750" checksum="57FDB474CBBD60D4" modified="2023-09-13" version="1">MRTLDLFLLVFWSDQFCDSSFSTMKVQGLVALITGGVSGFGKGFAEALLEKGGRAVSLVDISDGKNVVDEFSNKFGADRVMYHHADVRSDSDMENAFERTVQAYRQLDVVCNNAGIANEIPDWKKVIDINLTAVLRGTFLAKKYMDKKSGGKGGLVINVSSIAGLMYLPHFPVYCAAKHGVIGYSRSLAMPGAASAFSPDNIRINVVCPSFADTPIFETSSPRVPLEKIKAFKNSLPMVPVSDVVAAYMRLVEEDLHGEAIRITREKGIDFHKFKDQPESDLSKIVFHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CBP3</accession>
    <name>A0A9Q1CBP3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_12690</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CBP3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8041783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="82" mass="9845" checksum="28195F0D775FCC6F" modified="2023-09-13" version="1">MYYRKRKNPIDFSSGQRSFGVIGGQTLKNLLTQYLKVESLDECHTKYVDALWEEEEPYCFWWRSKVIWGYWGQTEDLVNILS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CCC8</accession>
    <name>A0A9Q1CCC8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Transcriptional repressor scratch 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_13066</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8042084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CCC8"/>
    <dbReference type="OrthoDB" id="5428132at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000003">
        <property type="entry name" value="Zinc finger protein 3 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050527">
        <property type="entry name" value="Snail/Krueppel_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24388">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24388:SF100">
        <property type="entry name" value="ZINC FINGER PROTEIN 423"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="266"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="92"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8042084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="323" mass="35345" checksum="397EF736EDB863B1" modified="2023-09-13" version="1">MPRSFLIRKLAVTEDQRANSTSKWDCFAASTQKTDSSKNQPTPLNLQVGANSTEVPKVFHFHSVELPFNKGRDWSRAETRHPIQIGGAPVEQGTSASQSLTSPSNNVFGNQGLLYVATENGLLLLPNLSTAPPITGGPFSVQLSQLTDQNGHFLVDEKSIFFTASNHITQMDNATNGEPWKEKLQVTPSTNHSNTNFELTQGASHEVANSYTNISTQQGPQEAPLNLSISANSLSHQRPGASSLKEVLNVPAPRSVNPQNQPMHGYECSECGKTYSTSSNLARHRQTHRSVNDQKARKCPHCDKVYVSMPALSMHIRDTQVGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CD84</accession>
    <name>A0A9Q1CD84_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_09360</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8042578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CD84"/>
    <dbReference type="OrthoDB" id="10068277at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8042578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="243" mass="28290" checksum="25D43496D7D4CA3C" modified="2023-09-13" version="1">MEYAKKMMLTDPSELAKWQTTSTHRELHDVLSRMEKTMRAQETPNIQRQRRLEDKVDHLLKNKDVDDDVRAKLYQEALRRQRVHHKQIPSMKDVMSPVHDAEGHHVSDHAIIASVPKKYRAKAGRLLQFFKGRGITWNPQGELEMNDKAIKRSHVIDLVNDLMPHRESFEPRGHETLSHWSADLNIPEELVGNPEQKKAIQRLGDSKEVYAVASHLSTPPQTPNRKGMRRQPARTLTWEPYIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CEP8</accession>
    <name>A0A9Q1CEP8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_10409</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CEP8"/>
    <dbReference type="OrthoDB" id="2160826at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050688">
        <property type="entry name" value="Zinc_finger/UBP_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24403:SF67">
        <property type="entry name" value="FI01116P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24403">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="106"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="135"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="32"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="150"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="150"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="203"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8043358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="630" mass="72684" checksum="675B2210D2877DF3" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CF58</accession>
    <name>A0A9Q1CF58_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Serine/threonine-protein kinase D3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_11059</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CF58"/>
    <dbReference type="OrthoDB" id="6097776at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00180">
        <property type="entry name" value="PKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF24">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE CHK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="56"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8043795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="319" mass="35854" checksum="CE1D6DF75FC8E346" modified="2023-09-13" version="1">MCHGNSALEWNVAKDFPVEELLEQDKIPSVHIKFNLPVSCPEIPWSDLEDVLDRRTKKPKILGSGSFGIVKLMKHQSSGKLCAVKINKDDIEEPTVIENLQLEVGVLTRLKHLDCVPELFGVTIPGNNSQVPVMVQEFIGDPKTFRSTMLAIAIEAKVLPPLKAKRVALRICEALRDIHSAKLLHCDLKNDNIMLEPGFVRKDNPKVKIIDFGKAVKMDDKPRYQYNARDQMSWIMKCCSHLAPEVVRGEKPYNVSSEVYGLGVVLKDLSKHFRGFLWQLGEECTDADYRKRPSVEDVIHKIKSHLTSIQGAASQLAAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CG05</accession>
    <name>A0A9Q1CG05_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Fucosyltransferase</fullName>
            <ecNumber evidence="11">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_11318</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Golgi apparatus</location>
            <location evidence="11">Golgi stack membrane</location>
            <topology evidence="11">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the glycosyltransferase 10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="11"/>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CG05"/>
    <dbReference type="OrthoDB" id="5790915at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046920">
        <property type="term" value="F:alpha-(1-&gt;3)-fucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11660:FF:000004">
        <property type="entry name" value="Glycoprotein 3-alpha-L-fucosyltransferase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11660">
        <property type="entry name" value="Glycosyl transferase family 10, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055270">
        <property type="entry name" value="Glyco_tran_10_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031481">
        <property type="entry name" value="Glyco_tran_10_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001503">
        <property type="entry name" value="Glyco_trans_10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038577">
        <property type="entry name" value="GT10-like_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11929">
        <property type="entry name" value="ALPHA- 1,3 -FUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11929:SF145">
        <property type="entry name" value="ALPHA-(1,3)-FUCOSYLTRANSFERASE FUT-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17039">
        <property type="entry name" value="Glyco_tran_10_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00852">
        <property type="entry name" value="Glyco_transf_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="18"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="domain" description="Fucosyltransferase N-terminal" evidence="13">
        <location>
            <begin position="157"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="Fucosyltransferase C-terminal" evidence="12">
        <location>
            <begin position="258"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF17039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ8043985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="484" mass="56394" checksum="17494838F2ABCF88" modified="2023-09-13" version="1">MGSTDASLKIKNQINPKLCFIALSCIVFVIVLSITYICMEHVTWDYSSSYPTFKISDTVEEIRYFESIMTEHDPFDVNFQIWLENKDMHVNPTHDGSRALCRTRTKPLRCPANNVIECLKISVVGLCGRWATKLRSLPAEISCPGLKNTKVLLDHVDKTKAIQEAHIVLFSFVASYLNLDIWLKLGFYRSPGQMWVLQTEESPIYARGLMMPWFFRNDTYNISNTYHTRADIHSPYGYYEPFRNEKSNPAVNLTKVIEEKKNFIIWISSHCETLQWDRTKLVHDLQRVTKVDIFGKCGNISICGDKWETTCSLNVDKIVRSYKFSLALENSCCEDYVTEKLWHTLSIGTVPIVIGPQIENYKKLAPPGSFIHVDQFDSVESLKDYLEYLDSNDTAYLEYFKWKESGIVREHSIKEHYQNHLSDDVICAIAEQYFKRRSSPTEFKMFDLYGPEWKGTCHYCGNHNWIRNYEHPLDHKRRNKGIWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CHP0</accession>
    <name>A0A9Q1CHP0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Usherin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">HOLleu_08083</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">Nanhai2018</strain>
            <tissue evidence="19">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
            <location evidence="2">Extracellular space</location>
            <location evidence="2">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="5984158at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00055">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="29"/>
    </dbReference>
    <dbReference type="CDD" id="cd00110">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000090">
        <property type="entry name" value="laminin subunit alpha"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000135">
        <property type="entry name" value="Laminin subunit beta 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000224">
        <property type="entry name" value="Usherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000275">
        <property type="entry name" value="usherin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001004">
        <property type="entry name" value="Usherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001030">
        <property type="entry name" value="Usherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001100">
        <property type="entry name" value="Usherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001176">
        <property type="entry name" value="Usherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001379">
        <property type="entry name" value="Usherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001716">
        <property type="entry name" value="Usherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="32"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006558">
        <property type="entry name" value="LamG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001791">
        <property type="entry name" value="Laminin_G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008211">
        <property type="entry name" value="Laminin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002049">
        <property type="entry name" value="LE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050713">
        <property type="entry name" value="RTP_Phos/Ushers"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46957">
        <property type="entry name" value="CYTOKINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46957:SF7">
        <property type="entry name" value="USHERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00053">
        <property type="entry name" value="EGF_laminin"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02210">
        <property type="entry name" value="Laminin_G_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13385">
        <property type="entry name" value="Laminin_G_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00055">
        <property type="entry name" value="Laminin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00011">
        <property type="entry name" value="EGFLAMININ"/>
    </dbReference>
    <dbReference type="SMART" id="SM00180">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="33"/>
    </dbReference>
    <dbReference type="SMART" id="SM00282">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00560">
        <property type="entry name" value="LamGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00136">
        <property type="entry name" value="LamNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01248">
        <property type="entry name" value="EGF_LAM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50027">
        <property type="entry name" value="EGF_LAM_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="30"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50025">
        <property type="entry name" value="LAM_G_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51117">
        <property type="entry name" value="LAMININ_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 12" id="KW-0424">Laminin EGF-like domain</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="5095"/>
            <end position="5118"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin N-terminal" evidence="18">
        <location>
            <begin position="332"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="16">
        <location>
            <begin position="685"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="16">
        <location>
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            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8044829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="136" mass="15448" checksum="FAFD0D2237555B98" modified="2023-09-13" version="1">MVTQTVNTGDNGVTIQMTVRNVAQDRLNWRHNEYNLPTQSTSDRRTYSISGTIQKRDAGVYICYESNRFDEARHGMQRLIVRGVAKQIAGVLTALVSVITVTMEEFVMMRLGDVSVLQDSWEHTVLQVLNILLFTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CI21</accession>
    <name>A0A9Q1CI21_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Neurexin-1a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_08011</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CI21"/>
    <dbReference type="OrthoDB" id="6275838at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00110">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001791">
        <property type="entry name" value="Laminin_G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050372">
        <property type="entry name" value="Neurexin-related_CASP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15036">
        <property type="entry name" value="PIKACHURIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15036:SF85">
        <property type="entry name" value="SP2353, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02210">
        <property type="entry name" value="Laminin_G_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00282">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50025">
        <property type="entry name" value="LAM_G_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Laminin G" evidence="2">
        <location>
            <begin position="98"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8045080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="299" mass="33457" checksum="966674DF8ADA82FD" modified="2023-09-13" version="1">MENIGTLIVVIDGSCEIIAFKNTQEPAPEDFSGDALDHHLIDIGGFVDPDTRPGSVTGFKGYIEHFIYNGRYYFELAANEGYSNVEISASREAIQPRPILTPITFQTKEVFAELQTQSTFQGLTFFFQFRTTEADGLILFDRGDEQDFIAVEILGGRLHYVFNQGSGTTSIQDVTMRPLNDNMWHEVWIYRDSNDQHILMVDGVPAADRAASGASTLNLGGLLRVGGLQESEFSDVPEMVQSMKGYQGCFASLEINYEPIDIFDSADSIESKEDLIEGCTGNSHFLFCVNLHFFLWHWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CI22</accession>
    <name>A0A9Q1CI22_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Betaine--homocysteine S-methyltransferase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_08310</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="6">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (BhmT route): step 1/1.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CI22"/>
    <dbReference type="OrthoDB" id="261426at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047150">
        <property type="term" value="F:betaine-homocysteine S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.330:FF:000003">
        <property type="entry name" value="Betaine--homocysteine S-methyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.330">
        <property type="entry name" value="Homocysteine-binding-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051524">
        <property type="entry name" value="BHMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017226">
        <property type="entry name" value="BHMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003726">
        <property type="entry name" value="HCY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036589">
        <property type="entry name" value="HCY_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46120">
        <property type="entry name" value="BETAINE--HOMOCYSTEINE S-METHYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46120:SF4">
        <property type="entry name" value="HCY-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02574">
        <property type="entry name" value="S-methyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037505">
        <property type="entry name" value="Betaine_HMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82282">
        <property type="entry name" value="Homocysteine S-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50970">
        <property type="entry name" value="HCY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 6 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Hcy-binding" evidence="9">
        <location>
            <begin position="5"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="317"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="317"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="333"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="299"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="300"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR037505-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8045321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="376" mass="42094" checksum="819A76E17559477E" modified="2023-09-13" version="1">MEGSSGGLLERLAKGPVVGDGSFVVTLERRGYVDIGFCTPEATVQYPEAVTQLHREFLRAGADVMQTFSYNSSDNCLSFKNINEDNETINIFQCDNLSRRACDLAREVAKEGNALVAGSVSPVVEYMTLKEKKGAIEEFKKQIEIFIEKKVDFLIGEFFGHIFEAEWAIEAMKKSGMPVACTMKIGPAGDLSGISPKECAVRMAKAGADLVGINCNYDPFICLETIKMMKDALEEEGLSCYLMAQPVGWHTQEIRNDVRGYNALPEWPLAMESRLVTRTDVRKFARAAYDLGVSYIGGCCGFEPYHIRAIAEELAEERGRRPPGKDKSCNYHSLRNSVRQNQRNRSDRKYWENLNPATGRKNARVLAKLDETPWIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CIK5</accession>
    <name>A0A9Q1CIK5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_09072</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CIK5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="5"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="225"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="225"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="247"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8045948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="297" mass="33042" checksum="6FC47699D193336C" modified="2023-09-13" version="1">MLQIYGRYSGRGHHNQQFEYSGGRGRYHRNFDGDGGKGNRKGRGTRNFYRGSDRRHDENRNVGQNRVDVTPSAEEECASPLSNPMDQWEPSGGEDWETAEDKILAESPSAPEGELWKPSPNQDPLSGPIGTWEPPEEERQWEEDMENNKMSVGEKVQTWQHSGVLGGIPLDQIDNDMGPSMDVPLSKPDAAHLPEEDAAALVDAEASHLVENIQIGEDVDDRLKENKDNVKGKRHLEPLIIPERDGQPSNVTSPLSAGSLKTPTDLHQDWSVSPVGFPSQGLHTVEEGTNVYQFDMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CIW2</accession>
    <name>A0A9Q1CIW2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Deubiquitinase DESI2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_08810</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DeSI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CIW2"/>
    <dbReference type="OrthoDB" id="412286at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0101005">
        <property type="term" value="F:deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1720.30">
        <property type="entry name" value="PPPDE domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008580">
        <property type="entry name" value="PPPDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042266">
        <property type="entry name" value="PPPDE_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12378">
        <property type="entry name" value="DESUMOYLATING ISOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12378:SF80">
        <property type="entry name" value="IP06716P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05903">
        <property type="entry name" value="Peptidase_C97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01179">
        <property type="entry name" value="DUF862"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51858">
        <property type="entry name" value="PPPDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPPDE" evidence="5">
        <location>
            <begin position="16"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="171"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8045745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="192" mass="21683" checksum="F9AE45DBA01DB782" modified="2023-09-13" version="1">MSSSDLSEDQDDVNPALVKLNIYDMYWTNDYTTTIGLGVFHTGVEVYGREFAFGGHPYPLSGIFEIYPRDIDDLGPQFRYKETIELDVTDLPEEDVSKVVECLGKKYPGDGYHLIHKNCNHFAEELVQILCNKPIPSWINRLASAGARLPFLEKMLPKEWLTPLALEEAAGEGDENCENEGRHVHLNNGKPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CJR2</accession>
    <name>A0A9Q1CJR2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_09162</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8046013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CJR2"/>
    <dbReference type="OrthoDB" id="10454012at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="111"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="194"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8046013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="337" mass="37925" checksum="FF9C893651E5A211" modified="2023-09-13" version="1">MNGTQDFCPTSSLQNVDLPTNDATPPDAHVIEGDLTEEGNSNRSDLMSVEALINKYASHSRETIPSESCELDEHSVVNFKDEDFTNEVGFHENRPTENGKDEYLLPSIPAKRVPSASDGKDTCVSSSNDTPEDAGEETFKEGNDHHEKLENNTFENRQEQDVSTNHGPNPVKPLMPQIRDLAFQLTDQIHRQTVEETENSTDVGNEETKKKKVSWQVGGTKPKKTTKKAAASVFRGSMASTVTTIAGLKKWKLKARQDTQSDFEKYPGHLRVVQEHLRQEKTETPLTIRQDVKKEVLPILANIVRAYKKELVGEHRLLREATLKYEKLKSELQDAFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CL20</accession>
    <name>A0A9Q1CL20_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNA exonuclease 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_06548</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the REXO4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CL20"/>
    <dbReference type="OrthoDB" id="8191639at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06144">
        <property type="entry name" value="REX4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000007">
        <property type="entry name" value="Interferon-stimulated exonuclease gene 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037431">
        <property type="entry name" value="REX4_DEDDh_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047021">
        <property type="entry name" value="REXO1/3/4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Ribonucl_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12801:SF158">
        <property type="entry name" value="RNA EXONUCLEASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12801">
        <property type="entry name" value="RNA EXONUCLEASE REXO1 / RECO3 FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00929">
        <property type="entry name" value="RNase_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exonuclease" evidence="9">
        <location>
            <begin position="306"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="174"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="279"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="472"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="22"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="37"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="91"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="115"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="129"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="182"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="207"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="280"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8047522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="494" mass="56301" checksum="9D8FDFB3D8517432" modified="2023-09-13" version="1">MSGPSKSNANQRNSNFWWYMKHGSKKGRKRKKQLGNNKDKSNAEEEVITKGREKEREVKKSITGVGTGAIGTDSISEVIASKQARKRKLKREAAKQEKLEKKKKKLQLNGQGTFGKDKEQERTSVEQRRRTKRFKRKRHKRKNDLEGSISHLVVTAGETSLVDEYTSSNIEKSAVLKMPSKGPEENVNKSEKCKKEPWSAKMQIKGDTSRSGDKTHDTNKDVEVDKNWKALMKKINYQPNKNLREEGKKVKLGQMEEGLKGHQEIWFDDVDPELLEDDEMTSKSETRKEGNPDLIKGGTPSTSLTKYIGIDCEMVGIGPNGEDHMLARVSLVNQHGSCIYDKFVAPSESITDYRTSVSGITPEKLKNAEDFATAQKEVYDIIKGRILVGHAVQNDLKVLFLSHKRSMIRDTSTYKPFQELCKTKRPSLKKLSRMILDVTVQRSSHDSVEDAQAAMKLFMLHRQAWEKDMKKTHKHREETEASDKMKEISVLHSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CMB0</accession>
    <name>A0A9Q1CMB0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_00482</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CMB0"/>
    <dbReference type="OrthoDB" id="7444419at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8048242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="116" mass="13219" checksum="FD39DF6A5BD5A488" modified="2023-09-13" version="1">MKLKSLFELCDGNALDAIQFSMLLAPNKGLKSALEILKSRCGNPLGVVQAWIRNITRSPKLDSSNIRKFADDLDNCIKALTSLDCINEMDNQQSNRTIVEKLPRFTQHRWTKELDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CME0</accession>
    <name>A0A9Q1CME0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Pseudouridylate synthase 7-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_06629</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a uridine in tRNA = a pseudouridine in tRNA</text>
            <dbReference type="Rhea" id="RHEA:54572"/>
            <dbReference type="Rhea" id="RHEA-COMP:13339"/>
            <dbReference type="Rhea" id="RHEA-COMP:13934"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the pseudouridine synthase TruD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CME0"/>
    <dbReference type="OrthoDB" id="447290at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001522">
        <property type="term" value="P:pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02576">
        <property type="entry name" value="PseudoU_synth_ScPUS7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2350.20:FF:000003">
        <property type="entry name" value="Pseudouridylate synthase 7 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.20">
        <property type="entry name" value="TruD, catalytic domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01082">
        <property type="entry name" value="TruD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001656">
        <property type="entry name" value="PsdUridine_synth_TruD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011760">
        <property type="entry name" value="PsdUridine_synth_TruD_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042214">
        <property type="entry name" value="TruD_catalytic"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00094">
        <property type="entry name" value="tRNA_TruD_broad"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13326:SF31">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE 7 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13326">
        <property type="entry name" value="TRNA PSEUDOURIDINE SYNTHASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01142">
        <property type="entry name" value="TruD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50984">
        <property type="entry name" value="TRUD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="TRUD" evidence="6">
        <location>
            <begin position="714"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="105"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="164"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="374"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="167"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="374"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="422"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00036943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8047590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1016" mass="111151" checksum="5D96E743CC9E8262" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CMN2</accession>
    <name>A0A9Q1CMN2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_06312</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CMN2"/>
    <dbReference type="OrthoDB" id="5334309at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040161659" evidence="1">
        <location>
            <begin position="22"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8047334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="414" mass="47294" checksum="022526DED843A6B4" modified="2023-09-13" version="1">MEYLLLCCLMFIFTIFLNDRGHEILNDPEMKSKVGEKRMQNERIFSPKKLYNSTFYEVSESATGVILRQRHHSVASTQKQDFCEVVGGLHHATTGTIAKLTIEINKTCAREHKSDEVSHFSVIAVGKNHIFSFDPSAIEEGSTGTDFTCTPAYPGTYDLYVENIVYKGQWQVSGSPFQLVVEADALTKISYNGESPSCQTLQIPRTNLSWVEGHWLTRELTRDGQDTLRSGWVYQPKACNFDIFTKSNITMAAASPVPKTIVVLGTSTERGIFLSLIDLALREEEKRHFKDSDLGKCWGFVELQLGNLQFIFQDFRVELSRQANECDDKRVKITCHDEKKVEDYKIFDDAMGFLKEFLFNDRIWSDLSLMCVCDVSQLRVIINEIPSSWNGTFYVLSGFKNYRRSLYTRSGRKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CNW5</accession>
    <name>A0A9Q1CNW5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_01085</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CNW5"/>
    <dbReference type="OrthoDB" id="6121668at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8048678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="161" mass="17750" checksum="65241B68A4977AFC" modified="2023-09-13" version="1">MDTLENVLQSPHAPSPYPKAIRVLIADTNLQDDVLTVGITDGMEVAKVICYEKNFHAKLAKNKTVLLRNFQKGRSCLIINRSSVVLPATPLQNITETIIAQASCVVNPPPPQAVALKDVKAPKPGVQSPLITVTGEVVQVRFFKLKNARLAYYTTKYSLYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CP43</accession>
    <name>A0A9Q1CP43_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_00804</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CP43"/>
    <dbReference type="OrthoDB" id="6159292at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46704:SF9">
        <property type="entry name" value="BHLH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46704">
        <property type="entry name" value="CXC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8048475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="486" mass="54202" checksum="57451F4DD5170F26" modified="2023-09-13" version="1">MSELSTSHPDVHQKLMAGEFVVQRQSQYAFSQLACDQAIEQTCNRDTKTKGGLTGFSINKGAVHRWMLSSHERATITSECEAMAGKNPTFRSRKDLDKTRVHKDEEHTKDVIATIDSMINPFETDRSELVHISSGVVAPADVTDDMVSAHSEGEGAFQVFCEERFQHGSKDMFATLPRQKLVIGNSRQIDVREMMKYSLGPLPLSLATCQGSLVKSNKAKLMNFFEGGPEVPPHIEAPPNESVWIIDGMALLQQIKCSGITFGVLADQILRMLLSFARSNGSKFVHFVTDRYMNNSIKNAERVKRGSCGVELIKIYADEQSVPKQWKKFLSGGSNKEALIEYLFKKWSNESNTLGDVTIFVAHGPVCHKLFQSPGSSNVSVEEVNALHCNHEEADTRMFLHCAYAVNVTQCRNIIFKSPDTDVMVIALSMCTTIQAKLYFSTGVGNKKRIIDINQIADNLGQGMCYHTGMQAKHGKRREQIDFFST</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CP98</accession>
    <name>A0A9Q1CP98_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">procollagen-proline 3-dioxygenase</fullName>
            <ecNumber evidence="4">1.14.11.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HOLleu_01400</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Nanhai2018</strain>
            <tissue evidence="16">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the leprecan family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.7" evidence="4"/>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CP98"/>
    <dbReference type="OrthoDB" id="8517835at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031418">
        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019797">
        <property type="term" value="F:procollagen-proline 3-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032963">
        <property type="term" value="P:collagen metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056585">
        <property type="entry name" value="Leprecan_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039575">
        <property type="entry name" value="P3H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006620">
        <property type="entry name" value="Pro_4_hyd_alph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044862">
        <property type="entry name" value="Pro_4_hyd_alph_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14049">
        <property type="entry name" value="LEPRECAN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14049:SF9">
        <property type="entry name" value="PROCOLLAGEN-PROLINE 3-DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13640">
        <property type="entry name" value="2OG-FeII_Oxy_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23557">
        <property type="entry name" value="TPR_leprecan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00702">
        <property type="entry name" value="P4Hc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="15">
        <location>
            <begin position="759"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="391"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="397"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="411"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="478"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="542"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="568"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="579"/>
            <end position="618"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ8048896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="921" mass="107526" checksum="FF3DB4D5624007D4" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CRF1</accession>
    <name>A0A9Q1CRF1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_02989</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CRF1"/>
    <dbReference type="OrthoDB" id="10060618at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="164"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="174"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8049991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="193" mass="21666" checksum="B29FF2A0589E99B2" modified="2023-09-13" version="1">MKQSIQAILKHKSSTDDQHDHSLCPPGEDSWCGWQRDQASGSTDYKHAHGLPAAIIEAIRPTFEQLSKDDLLKKCLDGYTQNACESFNALIWKRCPKEVYCGKQTLMTAVAMAVSHFNDGAGSTEKILGILGVKNGTHTHRWSQNEDTQRLQASEKRIQLTEKKIRQSLRQKRKGEEEGHINKEGVTYEAGGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CRJ2</accession>
    <name>A0A9Q1CRJ2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_02612</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CRJ2"/>
    <dbReference type="OrthoDB" id="8964826at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8049735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="114" mass="13309" checksum="1CDA3ACD92D1676C" modified="2023-09-13" version="1">MGYRNYVDFVTYPQANSKLDAVLAKGEYYKVKPAPPISRSDHTTIHILPIFTEKHRESQPRKKKLKPDLSKDNVDILRDALDTTNWNVFRESSNNINELTEAVSCYINFVFFRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H075</accession>
    <name>A0A9Q1H075_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein POLR1D, isoform 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_30213</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H075"/>
    <dbReference type="OrthoDB" id="6352295at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038948">
        <property type="entry name" value="POLR1D-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34769">
        <property type="entry name" value="RCG42593, ISOFORM CRA_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34769:SF1">
        <property type="entry name" value="RNA POLYMERASE I AND III SUBUNIT D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8028080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="141" mass="16286" checksum="0649516E4664B165" modified="2023-09-13" version="1">MSTKCEGSNTGEDDELTRIALEELTKEAKRGKERSDTMGVAGWIKPQMRPNKRFLKNTLLGAARQRDAEKKKYHTEVKKSRSRGVSENFTSQELTGKKKEREQGEKSHEQHRHKSSKRHSSSENDRKISSKKKKRRTDDST</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H0R3</accession>
    <name>A0A9Q1H0R3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cystic fibrosis transmembrane conductance regulator</fullName>
            <ecNumber evidence="7">5.6.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="25">ATP-binding cassette sub-family C member 7</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="26">Channel conductance-controlling ATPase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="27">cAMP-dependent chloride channel</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="35" type="ORF">HOLleu_27302</name>
    </gene>
    <organism evidence="35 36">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="35" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="35">Nanhai2018</strain>
            <tissue evidence="35">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>ATP + H2O + closed Cl(-) channel = ADP + phosphate + open Cl(-) channel.</text>
            <dbReference type="EC" id="5.6.1.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="30">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="30">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>chloride(in) = chloride(out)</text>
            <dbReference type="Rhea" id="RHEA:29823"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>hydrogencarbonate(in) = hydrogencarbonate(out)</text>
            <dbReference type="Rhea" id="RHEA:28695"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Apical cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Early endosome membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Recycling endosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the ABC transporter superfamily. ABCC family. CFTR transporter (TC 3.A.1.202) subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="35">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.1.6" evidence="7"/>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055038">
        <property type="term" value="C:recycling endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005260">
        <property type="term" value="F:intracellularly ATP-gated chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000026">
        <property type="entry name" value="Multidrug resistance-associated protein lethal(2)03659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000014">
        <property type="entry name" value="Multidrug resistance-associated protein member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000163">
        <property type="entry name" value="Multidrug resistance-associated protein member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003144">
        <property type="entry name" value="Putative multidrug resistance-associated protein lethal(2)03659-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009147">
        <property type="entry name" value="CFTR/ABCC7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF456">
        <property type="entry name" value="MULTIDRUG RESISTANCE-ASSOCIATED PROTEIN LETHAL(2)03659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01851">
        <property type="entry name" value="CYSFIBREGLTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="21" id="KW-0868">Chloride</keyword>
    <keyword evidence="19" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="13" id="KW-0967">Endosome</keyword>
    <keyword evidence="20" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="23" id="KW-0407">Ion channel</keyword>
    <keyword evidence="17" id="KW-0406">Ion transport</keyword>
    <keyword evidence="22" id="KW-0413">Isomerase</keyword>
    <keyword evidence="18 32" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="36" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 32" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16 32" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="32">
        <location>
            <begin position="98"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="32">
        <location>
            <begin position="136"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="32">
        <location>
            <begin position="215"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="32">
        <location>
            <begin position="242"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="32">
        <location>
            <begin position="318"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="32">
        <location>
            <begin position="354"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="32">
        <location>
            <begin position="777"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="32">
        <location>
            <begin position="842"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="32">
        <location>
            <begin position="930"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="32">
        <location>
            <begin position="1026"/>
            <end position="1048"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="32">
        <location>
            <begin position="1060"/>
            <end position="1078"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="34">
        <location>
            <begin position="111"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="33">
        <location>
            <begin position="488"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="34">
        <location>
            <begin position="782"/>
            <end position="1086"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="33">
        <location>
            <begin position="1122"/>
            <end position="1355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="31">
        <location>
            <begin position="724"/>
            <end position="743"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00016668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00024167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00029720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00031358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00033163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00034073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00044653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00048778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="33">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="34">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="35">
        <source>
            <dbReference type="EMBL" id="KAJ8030792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="36">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1389" mass="156943" checksum="5195307EDA8953CC" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-10-08" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H820</accession>
    <name>A0A9Q1H820_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_19614</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8035821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10064731at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000032">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 297-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon gypsy-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054465">
        <property type="entry name" value="Integrase_p58-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF15">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22938">
        <property type="entry name" value="Integrase_p58_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="3">
        <location>
            <begin position="852"/>
            <end position="1010"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="321"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="585"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="685"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="78"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="321"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="336"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="360"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="585"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="629"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="701"/>
            <end position="725"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8035821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1380" mass="157455" checksum="7E0FA943DD15D156" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HA79</accession>
    <name>A0A9Q1HA79_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Endonuclease/reverse transcript</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_16195</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HA79"/>
    <dbReference type="OrthoDB" id="10065856at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33332">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8038699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="235" mass="27596" checksum="3192F09ADF2FE444" modified="2023-09-13" version="1">MHFNTKKCFSMRITHSRNPKLFNYTLDKDILESTASHTYLGDDISNNLTWNRHINHITSSANRSLAFIRRNLYSCPRSVKANAYLTLVRTLLEYSSSVWDPYTVSLSNKVEQIQRRAARFVCNDYSPHSSMTEILKDLGWDSLNVRRTVNRLAIFHKARLGFLALPMNNLQPVRRPSRHHHSNSFLHIPTNKDCYKYSFFPRTVRDWNLLPQNITDLEDPKQFKSAALRILRRDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HBA8</accession>
    <name>A0A9Q1HBA8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Hexosyltransferase</fullName>
            <ecNumber evidence="10">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_16834</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Golgi apparatus membrane</location>
            <topology evidence="1 10">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the glycosyltransferase 31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="10"/>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HBA8"/>
    <dbReference type="OrthoDB" id="2139606at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008499">
        <property type="term" value="F:N-acetyl-beta-D-glucosaminide beta-(1,3)-galactosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002659">
        <property type="entry name" value="Glyco_trans_31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214">
        <property type="entry name" value="BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214:SF376">
        <property type="entry name" value="HEXOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01762">
        <property type="entry name" value="Galactosyl_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8039193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="287" mass="32915" checksum="8B304003334C5132" modified="2023-09-13" version="1">MFYPSPKIFPVPIIRSPNVENCPKDKPFILIIVVNMADQSGHRNSIRRSWAKQTNFKPVSLRNDVSWWVVFSIGTVDGTEDSLKKEQENYGDLLQGDFPDHPSEETRKTLLGFKWAAEQLPASCQPLLVLKTECTVFVNTPLLTKWITDQMAQNTGIYAGKVIRGDVPIRDLRMSLQVPELDYPRSTFPPFVQGPCYLLSMDVLTTMLTLVRVVTPIAMEDAYIGLLAEKMDREPQNYDNFQVINRPKNQCHYLEMLFIKTGKATVHHQIFMVVNHQKEECLDKKDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HBN2</accession>
    <name>A0A9Q1HBN2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HOLleu_14294</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Nanhai2018</strain>
            <tissue evidence="15">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HBN2"/>
    <dbReference type="OrthoDB" id="6144703at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000185">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000102">
        <property type="entry name" value="Receptor-type tyrosine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050713">
        <property type="entry name" value="RTP_Phos/Ushers"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46957">
        <property type="entry name" value="CYTOKINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46957:SF3">
        <property type="entry name" value="CYTOKINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00014">
        <property type="entry name" value="FNTYPEIII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="15" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="14">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="14">
        <location>
            <begin position="44"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="14">
        <location>
            <begin position="149"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="14">
        <location>
            <begin position="252"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="14">
        <location>
            <begin position="348"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="12">
        <location>
            <begin position="572"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="13">
        <location>
            <begin position="771"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="12">
        <location>
            <begin position="886"/>
            <end position="1150"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="13">
        <location>
            <begin position="1066"/>
            <end position="1141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="509"/>
            <end position="528"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ8040095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1165" mass="132256" checksum="0260F98038D8EEAB" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HE29</accession>
    <name>A0A9Q1HE29_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Tyrosine-protein kinase CSK</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_09349</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6 11">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8042570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HE29"/>
    <dbReference type="OrthoDB" id="8897154at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004714">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013151">
        <property type="entry name" value="Immunoglobulin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00047">
        <property type="entry name" value="ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040357712" evidence="7">
        <location>
            <begin position="22"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="24"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00051243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8042570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="544" mass="62443" checksum="44733EA7CD88AFB1" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HEM0</accession>
    <name>A0A9Q1HEM0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_10123</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HEM0"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8043155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="146" mass="15953" checksum="017120506E20A381" modified="2023-09-13" version="1">MGAPAVCERSCGYGCTQQHVLNRTRYSRRELSSRASFLAHAELETGVAARKFRRCNEKSRAAARACLGKQPALQTRTSAGLVTRYSRRELRSRTSFLAHAELETGVAARKFRRCNEKSRAAARACLGKQPALQTRTSAGLVGGQPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HFW6</accession>
    <name>A0A9Q1HFW6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein NLRC5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_00516</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HFW6"/>
    <dbReference type="OrthoDB" id="427518at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312">
        <property type="entry name" value="NACHT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312:SF2">
        <property type="entry name" value="NUCLEOTIDE-BINDING OLIGOMERIZATION DOMAIN-CONTAINING PROTEIN 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NACHT" evidence="1">
        <location>
            <begin position="83"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8048267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="656" mass="75671" checksum="71F70E4BDDB2A254" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HG64</accession>
    <name>A0A9Q1HG64_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Reverse transcriptase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_11087</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09274">
        <property type="entry name" value="RNase_HI_RT_Ty3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22249">
        <property type="entry name" value="UDM1_RNF168_RNF169-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000115">
        <property type="entry name" value="Polyprotein of retroviral origin, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.370:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 17.6-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000032">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 297-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon gypsy-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054465">
        <property type="entry name" value="Integrase_p58-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041373">
        <property type="entry name" value="RT_RNaseH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22938">
        <property type="entry name" value="Integrase_p58_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17917">
        <property type="entry name" value="RT_RNaseH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="9">
        <location>
            <begin position="874"/>
            <end position="1032"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase" evidence="8">
        <location>
            <begin position="1288"/>
            <end position="1465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="94"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="343"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="607"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="707"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="343"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="358"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="382"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="607"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="631"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="651"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="676"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="723"/>
            <end position="747"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8043816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1680" mass="192387" checksum="7A7E99D55C906098" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HGQ3</accession>
    <name>A0A9Q1HGQ3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_08166</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HGQ3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8045210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="89" mass="9861" checksum="51176D95D7805540" modified="2023-09-13" version="1">MHKSSSVQNVKFEVEAGTAWLETPPPHYAPIGSLLRTKGNTELMDTITTCCGKCQCAVHPSTYNNLPTRTKVRADRNQLFTWLGLILTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HHR0</accession>
    <name>A0A9Q1HHR0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Adipocyte plasma membrane-associated protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_08376</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the strictosidine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HHR0"/>
    <dbReference type="OrthoDB" id="5307922at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018119">
        <property type="entry name" value="Strictosidine_synth_cons-reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10426:SF88">
        <property type="entry name" value="ADIPOCYTE PLASMA MEMBRANE-ASSOCIATED PROTEIN HEMOMUCIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10426">
        <property type="entry name" value="STRICTOSIDINE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20067">
        <property type="entry name" value="SSL_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03088">
        <property type="entry name" value="Str_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63829">
        <property type="entry name" value="Calcium-dependent phosphotriesterase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="Strictosidine synthase conserved region" evidence="6">
        <location>
            <begin position="216"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8045376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="515" mass="58729" checksum="40B50396C1E38742" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HI06</accession>
    <name>A0A9Q1HI06_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_06371</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HI06"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040245637" evidence="1">
        <location>
            <begin position="18"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8047384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="72" mass="8019" checksum="3117ED8871028106" modified="2023-09-13" version="1">MASRAASLALLFSMGSCCKLQEDWKFNFLTRKRIQVDTCSNPLGLRKAFAIPVKMGAIVQMFGQLQPTNQPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HJ01</accession>
    <name>A0A9Q1HJ01_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_05377</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HJ01"/>
    <dbReference type="OrthoDB" id="8047091at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45823">
        <property type="entry name" value="T-SNARE COILED-COIL HOMOLOGY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45823:SF1">
        <property type="entry name" value="T-SNARE COILED-COIL HOMOLOGY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8046633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="115" mass="13080" checksum="53CB89B89F3C4BB4" modified="2023-09-13" version="1">MAMCLAASLRGEAQAFLADLNIHARREYLSLVNALSRRCDPAHQTSVFRIQLKNLIRRKEESLPELAQEIRRLTRQVYPCAPSELQMYVWRCTKHDPNLGRSSQGITGDGVLSKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HJ78</accession>
    <name>A0A9Q1HJ78_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Recombining binding protein suppressor of hairless</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_01403</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Su(H) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HJ78"/>
    <dbReference type="OrthoDB" id="5600360at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01176">
        <property type="entry name" value="IPT_RBP-Jkappa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1450:FF:000001">
        <property type="entry name" value="Recombining binding protein suppressor of hairless"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000003">
        <property type="entry name" value="recombining binding protein suppressor of hairless"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000074">
        <property type="entry name" value="Recombining binding protein suppressor of hairless, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1450">
        <property type="entry name" value="LAG1, DNA binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015350">
        <property type="entry name" value="Beta-trefoil_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036358">
        <property type="entry name" value="BTD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040159">
        <property type="entry name" value="CLS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015351">
        <property type="entry name" value="RBP-J/Cbf11/Cbf12_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037095">
        <property type="entry name" value="RBP-J/Cbf11_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038007">
        <property type="entry name" value="RBP-Jkappa_IPT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10665">
        <property type="entry name" value="RECOMBINING BINDING PROTEIN SUPPRESSOR OF HAIRLESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09270">
        <property type="entry name" value="BTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09271">
        <property type="entry name" value="LAG1-DNAbind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20144">
        <property type="entry name" value="TIG_SUH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01268">
        <property type="entry name" value="BTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01267">
        <property type="entry name" value="LAG1_DNAbind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110217">
        <property type="entry name" value="DNA-binding protein LAG-1 (CSL)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0914">Notch signaling pathway</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="RBP-J/Cbf11/Cbf12 DNA binding" evidence="12">
        <location>
            <begin position="152"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-trefoil DNA-binding" evidence="13">
        <location>
            <begin position="291"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="72"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="82"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="100"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ8048899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="616" mass="67832" checksum="B9AF008E8256ECB9" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YC24</accession>
    <name>A0A9Q0YC24_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">VPS9 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_41888</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8020048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YC24"/>
    <dbReference type="OrthoDB" id="10028873at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.80">
        <property type="entry name" value="VPS9 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003123">
        <property type="entry name" value="VPS9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037191">
        <property type="entry name" value="VPS9_dom_sf"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51205">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="811"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="domain" description="VPS9" evidence="4">
        <location>
            <begin position="703"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="562"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="689"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="850"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="144"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="689"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="702"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="859"/>
            <end position="871"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8020048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="871" mass="100588" checksum="50D8EEC0528D8BD9" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YCH5</accession>
    <name>A0A9Q0YCH5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tyrosine-protein kinase ephrin type A/B receptor-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_41996</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8020128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YCH5"/>
    <dbReference type="OrthoDB" id="10062356at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.10">
        <property type="entry name" value="Tumor Necrosis Factor Receptor, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM01411">
        <property type="entry name" value="Ephrin_rec_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="192"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="219"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="245"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8020128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="286" mass="31662" checksum="3226A6258B2212AE" modified="2023-09-13" version="1">MDEGCLPCPAGGFYLDKVGSVSDEVGYIPCELCNPGTYVPLGIGNSSKSCQVCPEGTNKSRHAGFRACFCLDDYYRKNRFGKCLICPQDGVNCSGDIVHLRQGYYWHWTSSNIELYEKFKSNLQIFNDSYDREASSFNASLPLIYKCPVKENCINNVSEVKGNCRKGHHGWMCTVCEDGYIKLVGLCKECSSLLMVFLDIVIFLAPLIICLLGNYFSYYQLTSLFLVHFNITAAAAVVSGDISDAIFVLISVLNTAFAVLVAGKAIRKFSQNYMCTSIPTDNQSTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YDS3</accession>
    <name>A0A9Q0YDS3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_40549</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YDS3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="202"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="204"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8020848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="224" mass="26074" checksum="0FA7303564D4A034" modified="2023-09-13" version="1">MDVSFYLAGFTNVCVMRNIPDVENTSQSRRNRKTRSPAIFFDEKKKLILNEDISYIFGTYGGEGLQENYSNDLFIISGSTGEEKTISIPFCRRKCFNTYDGHYHCPICSFDLFEYYLIEGERPNTVDTCTWKRKTAFQQHLHKDHGAEKMDGMVTIARFKGKRSVPENILAKCPANYLQLVDEMKRRKRNFQPTLERYFKSKKARLDDSASSDDKESNPFSQEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YH03</accession>
    <name>A0A9Q0YH03_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Cell death protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_39225</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the peptidase C14A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YH03"/>
    <dbReference type="OrthoDB" id="6116485at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00032">
        <property type="entry name" value="CASc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1460">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001315">
        <property type="entry name" value="CARD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029030">
        <property type="entry name" value="Caspase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033139">
        <property type="entry name" value="Caspase_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016129">
        <property type="entry name" value="Caspase_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002398">
        <property type="entry name" value="Pept_C14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011600">
        <property type="entry name" value="Pept_C14_caspase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002138">
        <property type="entry name" value="Pept_C14_p10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001309">
        <property type="entry name" value="Pept_C14_p20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015917">
        <property type="entry name" value="Pept_C14A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47901">
        <property type="entry name" value="CASPASE RECRUITMENT DOMAIN-CONTAINING PROTEIN 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47901:SF8">
        <property type="entry name" value="CASPASE-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00619">
        <property type="entry name" value="CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00656">
        <property type="entry name" value="Peptidase_C14"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038001">
        <property type="entry name" value="Caspase_ICE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00376">
        <property type="entry name" value="IL1BCENZYME"/>
    </dbReference>
    <dbReference type="SMART" id="SM00114">
        <property type="entry name" value="CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00115">
        <property type="entry name" value="CASc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52129">
        <property type="entry name" value="Caspase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50209">
        <property type="entry name" value="CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01122">
        <property type="entry name" value="CASPASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01121">
        <property type="entry name" value="CASPASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50207">
        <property type="entry name" value="CASPASE_P10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50208">
        <property type="entry name" value="CASPASE_P20"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="6" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="CARD" evidence="12">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="Caspase family p20" evidence="11">
        <location>
            <begin position="153"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="Caspase family p20" evidence="11">
        <location>
            <begin position="209"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="Caspase family p10" evidence="10">
        <location>
            <begin position="382"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="86"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="95"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="284"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR038001-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8021894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="456" mass="51482" checksum="A3242D1BF1B88E97" modified="2023-09-13" version="1">MEDVHRKAIRANYVKLSKDMELDLVMASLIQSGIYEPFMVDTFKAKPGKLVQNQTFLNNIETRGPKAYAAFLNALVEAGQEHLAKDIKSKERPRPQNQFEPMETSNFPSHPTDAGVWPNPEGPAIFEQFHVKRAQVKSEMVDNDEVYRMVSQPRGLAVIINNRTFRTMPERRGTEVDGRNLQHVFKELGFNVETKSNVKAQVYRMSSKPRGLALIINNKHFDSMKTRQGTDIDAKNLVHVFQQLHFNVDVKANVTALEMATIVKEFCKMNHSNFDCVALAILTHGVEGALYGVDEKKLLVEDLLKYFDADRAPTLIGKPKIIILQACRGERFDKGIESTDATGAGYAEEVPPPVEDENADPDALAESMLKLELEAPDAFARFVSWRNSERGSWFIQAFTEVILQYAQSEDLLSMLTKVNAKVAKAFESSSGRNKQMPAPVTMLTKKLFFFPGFFRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YH10</accession>
    <name>A0A9Q0YH10_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">50 kDa hatching enzyme</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_37365</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="9">Can bind about 5 Ca(2+) ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M10A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YH10"/>
    <dbReference type="OrthoDB" id="406838at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:collagen catabolic process"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
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    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="CDD" id="cd04278">
        <property type="entry name" value="ZnMc_MMP"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Matrix metalloproteinase-16-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
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        <property type="entry name" value="M10A_MMP"/>
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        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
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        <property type="entry name" value="Pept_M10_metallopeptidase"/>
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        <property type="entry name" value="Pept_M10A"/>
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        <property type="entry name" value="Peptidase_Metallo"/>
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        <property type="entry name" value="Peptidoglycan-bd-like"/>
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        <property type="entry name" value="PGBD-like_sf"/>
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        <property type="entry name" value="MATRIX METALLOPROTEINASE"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="MATRIX METALLOPROTEINASE-21"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PG_binding_1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="MATRIXIN"/>
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        <property type="entry name" value="HX"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="5 8" id="KW-0862">Zinc</keyword>
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        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
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            <begin position="19"/>
            <end position="493"/>
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            <begin position="117"/>
            <end position="277"/>
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            <begin position="343"/>
            <end position="388"/>
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        <location>
            <position position="231"/>
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    </feature>
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        <location>
            <position position="97"/>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
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            <position position="172"/>
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        <ligand>
            <name>Ca(2+)</name>
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        <location>
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        <ligand>
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            <label>1</label>
        </ligand>
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    <feature type="binding site" evidence="9">
        <location>
            <position position="184"/>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
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        <location>
            <position position="189"/>
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        <ligand>
            <name>Ca(2+)</name>
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            <label>3</label>
        </ligand>
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        </ligand>
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            <name>Zn(2+)</name>
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        </ligand>
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        <location>
            <position position="207"/>
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        <ligand>
            <name>Zn(2+)</name>
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            <label>1</label>
        </ligand>
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            <name>Zn(2+)</name>
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            <name>Zn(2+)</name>
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        <ligand>
            <name>Ca(2+)</name>
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        <ligand>
            <name>Ca(2+)</name>
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            <label>5</label>
        </ligand>
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        <location>
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        </location>
        <ligand>
            <name>Ca(2+)</name>
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        </ligand>
    </feature>
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            <dbReference type="ARBA" id="ARBA00010370"/>
        </source>
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            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
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            <dbReference type="EMBL" id="KAJ8022463.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="493" mass="55847" checksum="D3D4292E822BC173" modified="2023-09-13" version="1">MCLLFCCYVLIIIIAAKGRTNGSLFDMSEQERMMEAEEYLKKYGYLPDIPETTSSPELLQTSPNFHEAVRLFQAYVGLEQTGELDNETLTFMESPRCGFKDFSSPGEDGEVLKYSTGTVSWPKNHLKYFIKEYSIKLRDEEVKEIFQDSFQQLWADVTPLSFAEVENEFDADIVIRFATRQHGDANPFDGLGRVLAHAWLPTSGRAHFDDDENWAGPTGYGKSLQQVAVHEIGHMLGMKHSSAPEAVMAPYFRSGRQYHISVLDEDDIVGIQSLYGAPTVSTTMTQFTTVSPVPNGACLDKVDAVTCSNVLQRCYVFNGPYCWSIEGVHRQVSIIDSVFLFSPSNVNAAFFSPSNGRTYILKGCYVYQYDENLNFVSRRRNSQVWQALPCSINAIFYSSNDGLTYAFKNSWVYAYNSGRLYGRIRIENLRWPNMPSELFTGIDAVSDVPPGGQFSDNFVYVFKGDHYYMMNRRTKFFSTDSYNVKGEIYTGCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YHA7</accession>
    <name>A0A9Q0YHA7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Hermansky-Pudlak syndrome 3 protein-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_38610</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="10255480at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="HPS3"/>
    </dbReference>
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        <property type="entry name" value="HPS3_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28633:SF1">
        <property type="entry name" value="BLOC-2 COMPLEX MEMBER HPS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28633">
        <property type="entry name" value="HERMANSKY-PUDLAK SYNDROME 3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14763">
        <property type="entry name" value="HPS3_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BLOC-2 complex member HPS3 C-terminal" evidence="2">
        <location>
            <begin position="4"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="BLOC-2 complex member HPS3 C-terminal" evidence="2">
        <location>
            <begin position="400"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="322"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="322"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8021420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="572" mass="65028" checksum="9065058DDE55402B" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YI95</accession>
    <name>A0A9Q0YI95_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Glutamate--cysteine ligase</fullName>
            <ecNumber evidence="3 10">6.3.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 10">Gamma-ECS</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8 10">Gamma-glutamylcysteine synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_40821</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-cysteine + L-glutamate + ATP = gamma-L-glutamyl-L-cysteine + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13285"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58173"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the glutamate--cysteine ligase type 3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.2" evidence="3 10"/>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8021059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YI95"/>
    <dbReference type="OrthoDB" id="7939818at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017109">
        <property type="term" value="C:glutamate-cysteine ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004357">
        <property type="term" value="F:glutamate-cysteine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006750">
        <property type="term" value="P:glutathione biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.590.50:FF:000002">
        <property type="entry name" value="Glutamate--cysteine ligase catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.590.50:FF:000001">
        <property type="entry name" value="Glutamate-cysteine ligase Gcs1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.960">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.590.50">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004308">
        <property type="entry name" value="GCS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11164">
        <property type="entry name" value="GLUTAMATE CYSTEINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11164:SF0">
        <property type="entry name" value="GLUTAMATE--CYSTEINE LIGASE CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03074">
        <property type="entry name" value="GCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 10" id="KW-0317">Glutathione biosynthesis</keyword>
    <keyword evidence="4 10 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU367135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8021059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="647" mass="73448" checksum="DAA45B4A93503C7E" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YIA8</accession>
    <name>A0A9Q0YIA8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Kelch-like protein 13</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_39387</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8022016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YIA8"/>
    <dbReference type="OrthoDB" id="45365at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14733">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056737">
        <property type="entry name" value="Beta-prop_ATRN-MKLN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017096">
        <property type="entry name" value="BTB-kelch_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632:SF17">
        <property type="entry name" value="KELCH-LIKE PROTEIN 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632">
        <property type="entry name" value="LD33804P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24981">
        <property type="entry name" value="Beta-prop_ATRN-LZTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037037">
        <property type="entry name" value="Kelch-like_protein_gigaxonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BTB" evidence="3">
        <location>
            <begin position="25"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8022016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="566" mass="63901" checksum="3352066D05CEF1F5" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YIR0</accession>
    <name>A0A9Q0YIR0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_35622</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YIR0"/>
    <dbReference type="OrthoDB" id="5851061at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8023258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="59" mass="6728" checksum="4D0AA5DD5080D73F" modified="2023-09-13" version="1">MLSFFEKRHNTKIVSHALHRIQGTNRAEALSEKTNTSTNLRIPLVISYHPAMSPIIRTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YJA3</accession>
    <name>A0A9Q0YJA3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BRICHOS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_35659</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YJA3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8023289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="260" mass="29455" checksum="DE7DAAA9DF53C8EB" modified="2023-09-13" version="1">MTSEKSQMATEAGKQNFFYNDERYHQLKLKLFGTLLVTLVLVAGFVGVVYIQSKTIQQLSVLDEDNGNQYKVVEETKQQVQTTEAEQDPRIYFFENVMGDLKILVDLDRDLTIIKNVTTGNCFLTYNENVPLIENGGKIDGDLQLEIIRKDNPEEHSVMDSVLRYDETAIIPPEYLTATTHKAARGMCNGHQSYWADNGRVAVPRNRRQAAVRPASSICCYETYSCCMGGYPAWCVRYTCSNCGVAAKSVATWDCDREYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YJT4</accession>
    <name>A0A9Q0YJT4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ZU5 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_36338</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YJT4"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005042">
        <property type="term" value="F:netrin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.220.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037936">
        <property type="entry name" value="UNC5A-D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000906">
        <property type="entry name" value="ZU5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12582">
        <property type="entry name" value="NETRIN RECEPTOR UNC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12582:SF41">
        <property type="entry name" value="UNC5C-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00791">
        <property type="entry name" value="ZU5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51145">
        <property type="entry name" value="ZU5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ZU5" evidence="1">
        <location>
            <begin position="395"/>
            <end position="506"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8023797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="506" mass="57150" checksum="A21C21EE192CB7D5" modified="2023-09-13" version="1">MAIHISQPTPILCIRHNRFKSDRCWRLSDVCVSETNVIAVSGYHSYTERTFIDLFNNISDSKSPQEVSSIEFEPHVGLKFRFVCFLEEDDNLVTSIGEQVNIVTPRENKTLKHRVLKGIVGYLTVWNGNIFVTFDDSSTVHEYDRQLNLLNVIQLKGIPHRDSCVSISFSPDAYFVCTTRGRAMKLSTKDGVAVYEFDDHTQQSAHGLSVSVSRGLAVIVWQWNDVRVYSIDDNSLILGFNVDDAVYRIRMSDRGTIATGYHYTGEVKVYSVTELLSRDQLKSKGIVDQHNVAMSKDQFKLEMKSSYSDTTDMFPKLRAPIIKQPQILSPTPSAEGECSASVKISNADPRTKRTQTDTYDSENDMATKRKQKILRATPVREEENQTQTSTENIFLKAEATIGQSGGTIGIPNTHVKLHIPPNALPEEVGNCVIQLKIIPQRMLKDKAATTFWNTPTVVELLPNNLQLREHVKLLFPHCFVLKGPRPSAKVLVSHQEQETQWCTFNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YLJ8</accession>
    <name>A0A9Q0YLJ8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_36017</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YLJ8"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040145902" evidence="1">
        <location>
            <begin position="19"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8023550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="64" mass="6838" checksum="F56F7187764C252E" modified="2023-09-13" version="1">MKILHLLAVLLVVGVMVSNNPVAGVCQMPDCYKCVRYKGDIICAFCVGDRATECDSTCEGCPPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YMY2</accession>
    <name>A0A9Q0YMY2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_32895</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YMY2"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040296323" description="Secreted protein" evidence="1">
        <location>
            <begin position="24"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8025357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="98" mass="11281" checksum="B234AA5AA7F11189" modified="2023-09-13" version="1">MNVLRQSFLSFCAVLMYAQTTTCTKLWGYLHNDYRQCTVACYKVCLHLNPSWQVNTHLLDYKIFVTSHHSPVVKQIISATNFKRTLANSGTQRPNGKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YN91</accession>
    <name>A0A9Q0YN91_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">D(2) dopamine receptor A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_33221</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YN91"/>
    <dbReference type="OrthoDB" id="10010417at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071880">
        <property type="term" value="P:adenylate cyclase-activating adrenergic receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043410">
        <property type="term" value="P:positive regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14967">
        <property type="entry name" value="7tmA_amine_R-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248">
        <property type="entry name" value="ADRENERGIC RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248:SF66">
        <property type="entry name" value="OCTOPAMINE RECEPTOR BETA-3R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="26"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="145"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="188"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="346"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="383"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="45"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="253"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="301"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8025618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="423" mass="46407" checksum="EECAC1425FC46D2E" modified="2023-09-13" version="1">MENITGTAFNELLEEPKEGSLRTSHIYRVFLTLLISIMCAFGVIGNCLVAFAIARYPNLRTVTNYLIVSLAVADTTVCAWVMPIAAYQDLNGGMWRLGNILCDLWVGSDVLMSTASIWNLCAVSVDRYLAISRPIWYATKRKITFALILISVAWSLAFMNSVMALFIVGGFQGNEDAEFCAVNTQPSFGIAASLLAFFIPACAILVLYALILRSAMRLTSSVRPLHTDPDQPSHSVEFSTRYAFTSKNQTKGTVGIANSANGESDVPTTVTTTGDNKQTSGIAKTVSFEAQASTMKCNEVPAPAASDQGPSQPSVMMEPTGGTNEDKRTKENKKRVKISMTRERRAALVIGIVVLAFIACWLPFFSVFFMISVCTTCTVSLGIFQFVTWLGWCNSALNPVIYTVFNREFRQAFRKIFHLKAVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YNQ6</accession>
    <name>A0A9Q0YNQ6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">tRNA(His) guanylyltransferase</fullName>
            <ecNumber evidence="12">2.7.7.79</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">tRNA-histidine guanylyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HOLleu_33466</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Nanhai2018</strain>
            <tissue evidence="17">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Adds a GMP to the 5'-end of tRNA(His) after transcription and RNase P cleavage. This step is essential for proper recognition of the tRNA and for the fidelity of protein synthesis. Also functions as a guanyl-nucleotide exchange factor/GEF for the MFN1 and MFN2 mitofusins thereby regulating mitochondrial fusion. By regulating both mitochondrial dynamics and bioenergetic function, it contributes to cell survival following oxidative stress.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 12">
            <text>a 5'-end ribonucleotide-tRNA(His) + GTP + ATP + H2O = a 5'-end phospho-guanosine-ribonucleotide-tRNA(His) + AMP + 2 diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54564"/>
            <dbReference type="Rhea" id="RHEA-COMP:14193"/>
            <dbReference type="Rhea" id="RHEA-COMP:14917"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:138282"/>
            <dbReference type="ChEBI" id="CHEBI:141847"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.7.79"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="14">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homotetramer. Interacts with MFN1 and MFN2; functions as a guanyl-nucleotide exchange factor/GEF for MFN2 and also probably MFN1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 12">Belongs to the tRNA(His) guanylyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.79" evidence="12"/>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YNQ6"/>
    <dbReference type="OrthoDB" id="62560at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008193">
        <property type="term" value="F:tRNA guanylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.3000:FF:000001">
        <property type="entry name" value="tRNA(His) guanylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025845">
        <property type="entry name" value="Thg1_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024956">
        <property type="entry name" value="tRNAHis_GuaTrfase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007537">
        <property type="entry name" value="tRNAHis_GuaTrfase_Thg1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038469">
        <property type="entry name" value="tRNAHis_GuaTrfase_Thg1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12729">
        <property type="entry name" value="TRNA(HIS) GUANYLYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12729:SF6">
        <property type="entry name" value="TRNA(HIS) GUANYLYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04446">
        <property type="entry name" value="Thg1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14413">
        <property type="entry name" value="Thg1C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028980">
        <property type="entry name" value="tRNAHis_guanylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12 13" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7 12 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 12 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 12 17" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 12" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNAHis guanylyltransferase catalytic" evidence="15">
        <location>
            <begin position="6"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="Thg1 C-terminal" evidence="16">
        <location>
            <begin position="138"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="29"/>
            <end position="34"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="75"/>
            <end position="76"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00058346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00065710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR028980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR028980-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR028980-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF04446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF14413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ8025808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="271" mass="31629" checksum="0D220C6EBC1A58B4" modified="2023-09-13" version="1">MANSKFEYVRNFEVVDKCLPNCWIVVRLDGKNFHRFSDDHEFVKPNDDRALDLMKKSAENVMNEFKDVVIAYGQSDEFSFVLKKTTTQFSRRASKLMSNMVSLFSSSFVYHWKDVFDHVPLLYPPAFDGRVVLYPSDQNLRDYLSWRQADCHINNLYNTCFWSLVQDAGCSPKDAEEKLKGSFSSDKNELLFSQFGINYNTLPEMHRKGSVLIWEMKDEITLQPQAKTSAVSDTNPAQREIIRQRRRVTCLHVDIIRDDFWNKHPEILSGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YNZ6</accession>
    <name>A0A9Q0YNZ6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_33618</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NDRG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YNZ6"/>
    <dbReference type="OrthoDB" id="191979at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004142">
        <property type="entry name" value="NDRG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11034">
        <property type="entry name" value="N-MYC DOWNSTREAM REGULATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03096">
        <property type="entry name" value="Ndr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="308"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="315"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8025914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="332" mass="37093" checksum="C00EC39D7A12A8DC" modified="2023-09-13" version="1">MSAGDSTANSADHQIKPHVVYTETFGAFHCYVQGNLSSHKVVCVTYHDIGLNHSAFIKFLDTNDMSAIKSRMCFVHINAPGQQDNAEKLAENYHFPTMQQLADVIPYVLKEVGVTDNHDLIGFGEGAGANILLRFSFSNPRVLGLILLECTSTAAGFMEWGQEKVASWNLTHRGMNPTAEKYLLWHHLGTHSSDMVDIVNSYRANLYERMNAHNLALFIDKFTNRNNVTVNLKDVKCPVLLATGKKSPHYKDVEQLFEHLSCTKNILCPDDVHGDIKEEAPSKLAESIILFLQGIHLLSGLPMRRLGRTPSLGETSKERTSSMSEMDRPEVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YP87</accession>
    <name>A0A9Q0YP87_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Arylsulfatase I</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_36853</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sulfatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8024194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YP87"/>
    <dbReference type="OrthoDB" id="103349at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008484">
        <property type="term" value="F:sulfuric ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16029">
        <property type="entry name" value="4-S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047115">
        <property type="entry name" value="ARSB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024607">
        <property type="entry name" value="Sulfatase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000917">
        <property type="entry name" value="Sulfatase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10342">
        <property type="entry name" value="ARYLSULFATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10342:SF273">
        <property type="entry name" value="RE14504P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00884">
        <property type="entry name" value="Sulfatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00523">
        <property type="entry name" value="SULFATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00149">
        <property type="entry name" value="SULFATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040188360" evidence="8">
        <location>
            <begin position="24"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="domain" description="Sulfatase N-terminal" evidence="9">
        <location>
            <begin position="29"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="490"/>
            <end position="514"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8024194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="514" mass="57815" checksum="691F3E5AFA01CE1F" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YPX8</accession>
    <name>A0A9Q0YPX8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_34205</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8026380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YPX8"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8026380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="136" mass="15494" checksum="3B1D74EC19E62B9B" modified="2023-09-13" version="1">MPHIERKNPIVFGGGQRSPGVSGGQTLKTLLTQYLEVGSLDEFHTLLSMWMCYGEKMDPIVFGGGQRSSGVTRGKKSKISQEFPRVLHGFTRGQRAKFSKISQDRHLESPEVKTQISQEFPRLNRNTKLYFSYMLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YPZ3</accession>
    <name>A0A9Q0YPZ3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_34135</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8026323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YPZ3"/>
    <dbReference type="OrthoDB" id="2968753at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009384">
        <property type="term" value="F:N-acylmannosamine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008761">
        <property type="term" value="F:UDP-N-acetylglucosamine 2-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006047">
        <property type="term" value="P:UDP-N-acetylglucosamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd24060">
        <property type="entry name" value="ASKHA_NBD_ROK_GNE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03786">
        <property type="entry name" value="GTB_UDP-GlcNAc_2-Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000013">
        <property type="entry name" value="Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000600">
        <property type="entry name" value="ROK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020004">
        <property type="entry name" value="UDP-GlcNAc_Epase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003331">
        <property type="entry name" value="UDP_GlcNAc_Epimerase_2_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03568">
        <property type="entry name" value="NeuC_NnaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18964:SF149">
        <property type="entry name" value="BIFUNCTIONAL UDP-N-ACETYLGLUCOSAMINE 2-EPIMERASE_N-ACETYLMANNOSAMINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18964">
        <property type="entry name" value="ROK (REPRESSOR, ORF, KINASE) FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02350">
        <property type="entry name" value="Epimerase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00480">
        <property type="entry name" value="ROK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="UDP-N-acetylglucosamine 2-epimerase" evidence="3">
        <location>
            <begin position="73"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="560"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="626"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>structural</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="636"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>structural</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="638"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>structural</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="643"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>structural</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR620004-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR620004-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8026323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="779" mass="85129" checksum="DD4C8646223D7C2A" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YQB0</accession>
    <name>A0A9Q0YQB0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_31602</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YQB0"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8026693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="89" mass="10333" checksum="D35D7CF136AC73A6" modified="2023-09-13" version="1">MCYGKKKVPIVFGGGQRSSGVTRGKTLKTLFIPYPKVESLDRFHTKYVDALWCEEEPYSFWWRSEVIWDHQGSKSENLVYTISQGRKLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YQS7</accession>
    <name>A0A9Q0YQS7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Polycomb protein SCMH1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_31680</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SCM family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YQS7"/>
    <dbReference type="OrthoDB" id="5912862at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09578">
        <property type="entry name" value="SAM_Scm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.190">
        <property type="entry name" value="SLED domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004092">
        <property type="entry name" value="Mbt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050548">
        <property type="entry name" value="PcG_chromatin_remod_factors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047531">
        <property type="entry name" value="SAM_Scm-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021987">
        <property type="entry name" value="SLED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038348">
        <property type="entry name" value="SLED_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12247">
        <property type="entry name" value="POLYCOMB GROUP PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12247:SF132">
        <property type="entry name" value="POLYCOMB PROTEIN SCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02820">
        <property type="entry name" value="MBT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12140">
        <property type="entry name" value="SLED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00561">
        <property type="entry name" value="MBT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51079">
        <property type="entry name" value="MBT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="repeat" description="MBT" evidence="8">
        <location>
            <begin position="14"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="repeat" description="MBT" evidence="8">
        <location>
            <begin position="120"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="10">
        <location>
            <begin position="447"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="221"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="390"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="232"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="251"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="394"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="430"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8026754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="521" mass="57628" checksum="962A79CAE0552539" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YS42</accession>
    <name>A0A9Q0YS42_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_33013</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YS42"/>
    <dbReference type="OrthoDB" id="775972at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45823">
        <property type="entry name" value="T-SNARE COILED-COIL HOMOLOGY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45823:SF1">
        <property type="entry name" value="T-SNARE COILED-COIL HOMOLOGY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8025452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="243" mass="27024" checksum="B301D32638E9D8D4" modified="2023-09-13" version="1">MANSEGEGEPLIDLGAESREMQQEEWAVLSSVGTEASRSEAGNEAENILVTEFHRHRPNITPLSNFGLTRENPDTQAGISSREATSQGHGGNPFLAQYGETDRREFSAHFQGEGRPPERYLGNDQHFRGLNSRNINRENTEVGGIPRYFGKPIMQPPQFDGSTNWDDYLVQFELISELNGWSPDTKALYLAAGLKGQARAILSELDAVRRRDFKELVTALGRRFGAASQTQLYKAILRNKANG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YU40</accession>
    <name>A0A9Q0YU40_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Dynactin subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HOLleu_34138</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Nanhai2018</strain>
            <tissue evidence="17">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cell cortex</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
            <location evidence="1">Centriole</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the dynactin 150 kDa subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8026326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YU40"/>
    <dbReference type="OrthoDB" id="2130750at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000132">
        <property type="term" value="P:establishment of mitotic spindle orientation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007097">
        <property type="term" value="P:nuclear migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.190">
        <property type="entry name" value="CAP Gly-rich-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036859">
        <property type="entry name" value="CAP-Gly_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000938">
        <property type="entry name" value="CAP-Gly_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022157">
        <property type="entry name" value="Dynactin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18916">
        <property type="entry name" value="DYNACTIN 1-RELATED MICROTUBULE-BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18916:SF6">
        <property type="entry name" value="DYNACTIN SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01302">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12455">
        <property type="entry name" value="Dynactin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01052">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74924">
        <property type="entry name" value="Cap-Gly domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00845">
        <property type="entry name" value="CAP_GLY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50245">
        <property type="entry name" value="CAP_GLY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0132">Cell division</keyword>
    <keyword evidence="11 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-0243">Dynein</keyword>
    <keyword evidence="8" id="KW-0493">Microtubule</keyword>
    <keyword evidence="9" id="KW-0498">Mitosis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CAP-Gly" evidence="16">
        <location>
            <begin position="27"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="76"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="346"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="823"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="924"/>
            <end position="1029"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="78"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="147"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ8026326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1278" mass="143158" checksum="A94E3738350CD709" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BAS6</accession>
    <name>A0A9Q1BAS6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_44606</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010001037">
        <property type="protein sequence ID" value="KAJ8017762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BAS6"/>
    <dbReference type="OrthoDB" id="10591380at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31389">
        <property type="entry name" value="LD39211P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31389:SF4">
        <property type="entry name" value="LD39211P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8017762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="164" mass="18666" checksum="680F6C4BA82D0FEC" modified="2023-09-13" version="1">MTISHIVHFLTRQQCLFITAFVLSFFFIVFFYASDSSLTAVSLFARNLPKPKAVFKKSNTIHPQAAIKTENDRNFAKNYDDYRATSTGKEIPIINLDKEEPPLNLLYEKLTLVTAFSENHFQEGIGFIGSAQQQMPNKTIIVYDLGLKEHSAKQVFDVLSFCFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BCZ9</accession>
    <name>A0A9Q1BCZ9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_40292</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BCZ9"/>
    <dbReference type="OrthoDB" id="10063152at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051055">
        <property type="entry name" value="PIF1_helicase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47642">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47642:SF5">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8020644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="389" mass="45991" checksum="77EC1B6E461279C6" modified="2023-09-13" version="1">MFPLTQTMILSPLTQDLPSEQLAQITANYKKISCELKNMKFGEDITFAQFLNRLQMSEQEYITAVRSSLTIEKVFLKRSPNEIRVNNYNTFLLRTWRANMDLQFVLHPYACAMYIVSYISKSQRGMSRLLQHAVQECKEGNSSIRESVRHIGNKFLNHIEMGAQEAVYYVLQIPLRKATRSFIFINTSPPENRSFLLKPLCKISELPDNSHDVESDTLLKKYVRRPRIMENICLADFAAWYDFSFEQDDELVDTLLEDDHPPEIISIQHNNQDNQIYHVGKMLIKKRSNPKIIRYVRYNEHNDSENHFREQLMLFIPWRKECNIIGNSTNFQQKYEKLKCQILIKARNYNKNNFAFEDLFVTENNNCMIMNRKLSTVPQMHHTGRHKMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BFI4</accession>
    <name>A0A9Q1BFI4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">WSC domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_36173</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WSCD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BFI4"/>
    <dbReference type="OrthoDB" id="5985073at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051589">
        <property type="entry name" value="Sialate-O-sulfotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45964:SF9">
        <property type="entry name" value="SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45964">
        <property type="entry name" value="WSCD FAMILY MEMBER CG9164"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sulfotransferase" evidence="2">
        <location>
            <begin position="210"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8023673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="371" mass="43019" checksum="EAE83E6DB51604D6" modified="2023-09-13" version="1">MEIPNTSGHNDDCLAAFGQSQTPVPAMDLKCVIYKSSKRVWLVVSIMTVSVLYLASHTYNWNNNVTVRHIKYQRPDVHADVTSGSGRAKGYIEDRPGNIIYPNVDISNFPTMDQVNCSGLIDDFDVAPAYSMPLIALASFPRSGNTWSRSLIQIATGYSTGSVFWKEERTYKRAKNRFKAGTEDFMLRKGVCVKTHKFEEDHIAIFDHGAILLIRNPYYSIVSEYFRFSRSYRNLTTEDIIRNMKEEDVTWENHCKRKFTKWKDTALSWIKHCKRLLVVFYENLEDNPILELSRMVTFLEQPIQPRRILCAVKFYSPMKGSQGHRSQLTFDPYTPEMHSILDEFIKTVNQTLILKNAFPLPTYQKYYLPQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BG09</accession>
    <name>A0A9Q1BG09_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transposable element Tcb2 transposase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_21358</name>
        <name evidence="2" type="ORF">HOLleu_34127</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8026316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BG09"/>
    <dbReference type="OrthoDB" id="6021633at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038717">
        <property type="entry name" value="Tc1-like_DDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047655">
        <property type="entry name" value="Transpos_IS630-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033545">
        <property type="entry name" value="transpos_IS630"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46564">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46564:SF1">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13358">
        <property type="entry name" value="DDE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tc1-like transposase DDE" evidence="1">
        <location>
            <begin position="154"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8026316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8034495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="340" mass="40314" checksum="6FD730313708E106" modified="2023-09-13" version="1">MPGTLVRPEVRLKIIRAAEIRRKKPYDIWKELRRTGVKISKETVKNIILRYRSRGHIRNLKRGGRRPRMTDEHAKLIHSLMEENKERTSVDLQKELLKRTGETFSTSQIRKKKRELGWVCYNTKYGQSIRHVNKVKRLIHIRELALTKETYHNVVFTDECTVELDNHARITFRKKDEKPVIKGRPKHPYKLHVWAGISKRGATPIFIFEGIMKQEFYVDILKQALVPFGRKTFPEGFRLMQDNDPKHTSNYAKRFMEEAGITWWKTPPESPDLNPIEKLWHEMKEFLRKEHKPTKKEELVQGIVLFWKQRVTPEKCRTYIGNLRKAIPAVVESLGMATRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BG18</accession>
    <name>A0A9Q1BG18_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">5'-nucleotidase</fullName>
            <ecNumber evidence="3">3.1.3.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_36451</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a ribonucleoside 5'-phosphate + H2O = a ribonucleoside + phosphate</text>
            <dbReference type="Rhea" id="RHEA:12484"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:18254"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
            <dbReference type="EC" id="3.1.3.5"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the 5'-nucleotidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.5" evidence="3"/>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BG18"/>
    <dbReference type="OrthoDB" id="7722975at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008253">
        <property type="term" value="F:5'-nucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006196">
        <property type="term" value="P:AMP catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07409">
        <property type="entry name" value="MPP_CD73_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.780.10:FF:000001">
        <property type="entry name" value="NT5E isoform 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000020">
        <property type="entry name" value="NT5E isoform 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.780.10">
        <property type="entry name" value="5'-Nucleotidase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008334">
        <property type="entry name" value="5'-Nucleotdase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036907">
        <property type="entry name" value="5'-Nucleotdase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006146">
        <property type="entry name" value="5'-Nucleotdase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006179">
        <property type="entry name" value="5_nucleotidase/apyrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11575:SF24">
        <property type="entry name" value="5'-NUCLEOTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11575">
        <property type="entry name" value="5'-NUCLEOTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02872">
        <property type="entry name" value="5_nucleotid_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01607">
        <property type="entry name" value="APYRASEFAMLY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55816">
        <property type="entry name" value="5'-nucleotidase (syn. UDP-sugar hydrolase), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00786">
        <property type="entry name" value="5_NUCLEOTIDASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="10">
        <location>
            <begin position="1"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="5'-Nucleotidase C-terminal" evidence="11">
        <location>
            <begin position="320"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="530"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="530"/>
            <end position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8023884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="569" mass="63283" checksum="3943F78F942E4E01" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BH91</accession>
    <name>A0A9Q1BH91_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_34605</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BH91"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8024647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="171" mass="18634" checksum="03C9EC39F83355B4" modified="2023-09-13" version="1">MRYGKRKNPIIFGGGQRSSGVTGGQSLKTLLTRYLKKPHTERKNPIVFGGGQRSPGVTGGQILKTLLTQYLEMRYGKRKNPFTFGGGQRSPGVTGGQTLKTLLTRYLEMPYSKRKNSIIFGGGQKSSGVTGGQTLKTLLTRYLEMPYSKRKNSIIFGGGQKSSGVTRGVKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BHC4</accession>
    <name>A0A9Q1BHC4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Fibronectin type-III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_35422</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8025264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BHC4"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040359094" description="Fibronectin type-III domain-containing protein" evidence="2">
        <location>
            <begin position="24"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="329"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8025264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="360" mass="40480" checksum="C110CE81F388DF86" modified="2023-09-13" version="1">MDFSWLFQFRVLIVFCLTSSVSWECGITHTPCDWMTLVSFEKCCNTSRVPINSVEIDYFVTRTDNSEERWRLDATLRWKAPTEDGAYMYSVDLVPTVTLEDLALYRIDAIENRPCQENEEETSNFTSILFGFRYEFQVQVIHNGVIPTAYTCFFCLEDPSLYLQAPDCLIATGDIEFCQNQPIPISTEPRDVKVDWTMESMSVIVNIKWSMPLVVNGKIAGYTIRARRFTTESIIFFKTTGNHTEIEITSESPGIRNGGFLAGYSYDILVAPIISLPAQYRSQGTTYEGNLAIRRISIPAIDVTTSKVIHTVTEGANPTTKSQGDSSDILPIALLLSSMSAVIVAIVAVSIMLLYIQKHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BHD6</accession>
    <name>A0A9Q1BHD6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_33718</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BHD6"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8025998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="52" mass="6160" checksum="A9724770C5FEC28C" modified="2023-09-13" version="1">MMAKSIEEYIKTYDRCFRYKATPTVSDRAPLVSIKTTEPMELVYGFSFTRRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BHV2</accession>
    <name>A0A9Q1BHV2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Apelin receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_32977</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BHV2"/>
    <dbReference type="OrthoDB" id="10037617at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00637">
        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45698:SF1">
        <property type="entry name" value="TRACE AMINE-ASSOCIATED RECEPTOR 13C-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45698">
        <property type="entry name" value="TRACE AMINE-ASSOCIATED RECEPTOR 19N-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="55"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="6">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8025427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="141" mass="16218" checksum="B11B47355DBA8C31" modified="2023-09-13" version="1">MSGAADKMAMELLEARKKIVKMLATIVITFAICWAPNQFIFFAYNLGWNLDFRSWYYHVSVLAAFCNSCMNPFIYAFQNGRYRRALHKAVFGKYVCFLKVGAESGEDATPSALSTNLFNQKRNEHQMIDSNKLKEETNFET</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BLD4</accession>
    <name>A0A9Q1BLD4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Integrase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_31057</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="8046937at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="CDD" id="cd01644">
        <property type="entry name" value="RT_pepA17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005312">
        <property type="entry name" value="DUF1759"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008042">
        <property type="entry name" value="Retrotrans_Pao"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331:SF5">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03564">
        <property type="entry name" value="DUF1759"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05380">
        <property type="entry name" value="Peptidase_A17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="243"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="253"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="277"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8028733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1774" mass="199569" checksum="058409C165AAE25A" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BM19</accession>
    <name>A0A9Q1BM19_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Solute carrier family 22 member 9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_28499</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BM19"/>
    <dbReference type="OrthoDB" id="2544694at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8029167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="193" mass="22419" checksum="D8744ADC56132CBA" modified="2023-09-13" version="1">MKFDGILKEIGEFGRFQIMVYTLVCIASAISAMVEFSQVFTSASVDHWCAVSEWSDEVDECQQKDQSLYLSCMHQLRDASIPLEEESERYSKCSRYDTDYPQNWYQGYSARNNTNITGSCEDGWVYDRSQYKSSAVMEVSQIVSLLELNYWAWVVGLVKTEKHDYVFSRRCMYVLQCSSFHSLFLMCFPLVGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BME0</accession>
    <name>A0A9Q1BME0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">DNA-directed RNA polymerase III subunit RPC3</fullName>
            <shortName evidence="7">RNA polymerase III subunit C3</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_28516</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the eukaryotic RPC3/POLR3C RNA polymerase subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BME0"/>
    <dbReference type="OrthoDB" id="272392at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000199">
        <property type="entry name" value="DNA-directed RNA polymerase III subunit RPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000218">
        <property type="entry name" value="DNA-directed RNA polymerase III subunit RPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000256">
        <property type="entry name" value="DNA-directed RNA polymerase III subunit RPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000262">
        <property type="entry name" value="DNA-directed RNA polymerase III subunit RPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055207">
        <property type="entry name" value="POLR3C_WHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013197">
        <property type="entry name" value="RNA_pol_III_RPC82-rel_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008806">
        <property type="entry name" value="RNA_pol_III_Rpc82_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039748">
        <property type="entry name" value="RPC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12949:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12949">
        <property type="entry name" value="RNA POLYMERASE III DNA DIRECTED -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08221">
        <property type="entry name" value="HTH_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05645">
        <property type="entry name" value="RNA_pol_Rpc82"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20912">
        <property type="entry name" value="RPC3_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22536">
        <property type="entry name" value="WHD_POLR3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7 12" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="RNA polymerase III subunit RPC82-related helix-turn-helix" evidence="10">
        <location>
            <begin position="8"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase III Rpc82 C -terminal" evidence="9">
        <location>
            <begin position="147"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed RNA polymerase III subunit RPC3 winged-helix" evidence="11">
        <location>
            <begin position="355"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="160"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="192"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="165"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="202"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF05645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF22536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8029181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="553" mass="62935" checksum="C87B891EB3486AEF" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BMT7</accession>
    <name>A0A9Q1BMT7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TATA box-binding protein-associated factor, RNA polymerase I, subunit C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_28787</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BMT7"/>
    <dbReference type="OrthoDB" id="2382881at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001650">
        <property type="term" value="C:fibrillar center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001164">
        <property type="term" value="F:RNA polymerase I core promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038801">
        <property type="entry name" value="TAF1C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049087">
        <property type="entry name" value="TAF1C_beta-prop"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15319">
        <property type="entry name" value="TATA BOX-BINDING PROTEIN ASSOCIATED FACTOR RNA POLYMERASE I SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15319:SF1">
        <property type="entry name" value="TATA BOX-BINDING PROTEIN-ASSOCIATED FACTOR RNA POLYMERASE I SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20641">
        <property type="entry name" value="TAF1C_beta-prop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TAF1C beta-propeller" evidence="2">
        <location>
            <begin position="252"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="570"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="636"/>
            <end position="656"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8029406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="879" mass="99546" checksum="244C6D08704BAFCF" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BN50</accession>
    <name>A0A9Q1BN50_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_29207</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BN50"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8029737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="104" mass="12035" checksum="63A53AEBB29C94A0" modified="2023-09-13" version="1">MSNTQDDLTSNLLQNVSKIHSQVRWGDRFCWENTSKASKKLTGIVRMVNIQPFSVKCCSQDREHFSDFHRSLQFFAKILDKVAEIEDDAKCYDVASDVYGKNIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BQB8</accession>
    <name>A0A9Q1BQB8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">General transcription factor II-I repeat domain-containing protein 2A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_27136</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BQB8"/>
    <dbReference type="OrthoDB" id="1101576at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45913">
        <property type="entry name" value="EPM2A-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45913:SF5">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR II-I REPEAT DOMAIN-CONTAINING PROTEIN 2A-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8030665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="366" mass="41484" checksum="EA088685D2BFDB26" modified="2023-09-13" version="1">MPDSHSCYMVVHAAIYPSYSTLLFIDFQPQPKKHKVQPSSSPTSHSILQQLSIRQPVETGSDCKCAKYSKLREKYWEMKECDTLKEQVQELTQYKARTVAMKDTTTGEDIFQATKTCIEDMGLEMEKLVSATTDGAPAMVGARKGAASLLENEMKNRGLNRELVKLHCIIHQEALCAKSATLKNVMNVVVKTVNLIRSKGLNHKQFQELLRETEGPYGDLLYHCEVRWLSQGDMLERVYDLRNCRIHGTERNGKSRVQRCRMDYTTCFPGGHYQTPVDVGHGIPTTGPNLFTALFVRSDVVRKSLTEKKSNAHLTTTESKAVPDLPRINFILGFTIRKYPNIPTTVLKQSLSDEIKEWDQPGKEEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BQL8</accession>
    <name>A0A9Q1BQL8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_27603</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8031012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BQL8"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040135239" evidence="1">
        <location>
            <begin position="24"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8031012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="312" mass="35259" checksum="94D6931D54D81D1F" modified="2023-09-13" version="1">MKPEMAFVHLLFAILLLISSADGTVTETIAKDEFWDGINAYVAVDREIIQKKLNEWHISNSGLCDAPKLYLPETPIFPGVIGANKHAVFIGVGNLTFHDFDPYADSHLWMAEAVTMVGVVIPLLADSPKGVPQYSLALGIYYEKNLGDFPKSNVMYPYFPVDEIRINNNRFVMRNGSEEMTVSLNRYTNPCKPTSPVVRHELDHLIRQEFHLGNPEPDFSFCDRHPIDNHFCETAKRIRCEVSRSVPNICQTPWHALKRHACQGTMEILNVTPILRDAMLLPPDPIEVFASEVLIGNFSIGLKYPCIENECM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BR65</accession>
    <name>A0A9Q1BR65_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_27695</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8031084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BR65"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8031084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="90" mass="10137" checksum="BB22CD66C939D515" modified="2023-09-13" version="1">MGEDICLPSVNGVFFSKDIPRSAIVHICSYQCFITKWLMEEHLTMEAVYSHQAKLSFIQSKGTAELLSSLKDFSGKATCKRWQGKEVFTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BSG4</accession>
    <name>A0A9Q1BSG4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_25298</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BSG4"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="172"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="283"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="127"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="183"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="235"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8031930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="327" mass="37360" checksum="5D17A66C210C77B6" modified="2023-09-13" version="1">MIVTMGGKSSKNNSWDTPQQPIQEISRNMPNSDMYESSIKWQPPYSVQNDAPTYINQHPHDIPNGAYGGSSLYYNQPQVSVKPTNQSLENSYANPVQTTAERFSAPTQHVEKSPKGQGKKTPKSQGKIKEKDVRKSSEESSSVTTFPDVHLPRAKFDREWYMEQLQERKHLQESVYEAFPENDEGRVGYNHEREEPHMNRAKDRSDSNPGIYTNKDKANRPLLYQGSKQHDNVSLVKTSRGNSQSGVDPYSAKYPSKESEASHPKPTALVQVLPSEPFEIRNLKRHQVHRGSPQNNNYFPEPDYNENRKSDWDFEYPSAEGIPEADY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BST4</accession>
    <name>A0A9Q1BST4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_25691</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BST4"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8032222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="78" mass="9053" checksum="1DF209DDC90DD092" modified="2023-09-13" version="1">MESSREFNFNAAFLEFSRRNCLRNATLLRNIGIVCLLTLVIIRSWNQFLRYSTLQVYYCTIVNVHALPTLGPDQANIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BTP2</accession>
    <name>A0A9Q1BTP2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Activated RNA polymerase II transcriptional coactivator p15</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_25904</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transcriptional coactivator PC4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BTP2"/>
    <dbReference type="OrthoDB" id="2505440at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060261">
        <property type="term" value="P:positive regulation of transcription initiation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.31.10">
        <property type="entry name" value="Transcriptional Coactivator Pc4, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003173">
        <property type="entry name" value="PC4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009044">
        <property type="entry name" value="ssDNA-bd_transcriptional_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045125">
        <property type="entry name" value="Sub1/Tcp4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13215">
        <property type="entry name" value="RNA POLYMERASE II TRANSCRIPTIONAL COACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02229">
        <property type="entry name" value="PC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54447">
        <property type="entry name" value="ssDNA-binding transcriptional regulator domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="WW" evidence="7">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8032389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="180" mass="20806" checksum="9EC3CB4E682F8724" modified="2023-09-13" version="1">MSDNWKQMKDNEGKKMYYNADTREATYDSPSQFKPVQTRVLPPWTAPMRTKGAWSPKRQYPYEQNVRDDEVRVSIGDDRYVTVSIFNGKLYIHIREFFFNQEGRMLPTSKGIALTPQQWFQLGEIQHVIDEAIQEASNGAKRRPSLSERRSASHLKLDLSSLDEDGCQPLNSDLNSEDTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BTX6</accession>
    <name>A0A9Q1BTX6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">F-actin monooxygenase</fullName>
            <ecNumber evidence="5">1.14.13.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="26" type="ORF">HOLleu_26165</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="26">Nanhai2018</strain>
            <tissue evidence="26">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-methionyl-[F-actin] + NADPH + O2 + H(+) = L-methionyl-(R)-S-oxide-[F-actin] + NADP(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:51308"/>
            <dbReference type="Rhea" id="RHEA-COMP:12953"/>
            <dbReference type="Rhea" id="RHEA-COMP:12956"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16044"/>
            <dbReference type="ChEBI" id="CHEBI:45764"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.14.13.225"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the Mical family.</text>
    </comment>
    <comment type="caution">
        <text evidence="26">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.13.225" evidence="5"/>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="20799at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120501">
        <property type="term" value="F:F-actin monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22198">
        <property type="entry name" value="CH_MICAL_EHBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000023">
        <property type="entry name" value="EH domain-binding protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000004">
        <property type="entry name" value="protein-methionine sulfoxide oxidase MICAL2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022735">
        <property type="entry name" value="bMERB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050540">
        <property type="entry name" value="F-actin_Monoox_Mical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057494">
        <property type="entry name" value="Rossman_Mical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23167:SF54">
        <property type="entry name" value="[F-ACTIN]-MONOOXYGENASE MICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23167">
        <property type="entry name" value="CALPONIN HOMOLOGY DOMAIN-CONTAINING PROTEIN DDB_G0272472-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12130">
        <property type="entry name" value="bMERB_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25413">
        <property type="entry name" value="Rossman_Mical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01203">
        <property type="entry name" value="DUF3585"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51848">
        <property type="entry name" value="BMERB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="18" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="17 21" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0967">Endosome</keyword>
    <keyword evidence="11" id="KW-0274">FAD</keyword>
    <keyword evidence="8" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="16 20" id="KW-0440">LIM domain</keyword>
    <keyword evidence="9 20" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="13" id="KW-0521">NADP</keyword>
    <keyword evidence="14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 20" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="23">
        <location>
            <begin position="517"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="24">
        <location>
            <begin position="781"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="domain" description="BMERB" evidence="25">
        <location>
            <begin position="1742"/>
            <end position="1891"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="641"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="848"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="937"/>
            <end position="1020"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="1102"/>
            <end position="1397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="1411"/>
            <end position="1650"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="1669"/>
            <end position="1693"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="21">
        <location>
            <begin position="1719"/>
            <end position="1779"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="22">
        <location>
            <begin position="658"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="671"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="848"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="22">
        <location>
            <begin position="887"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="22">
        <location>
            <begin position="946"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="1010"/>
            <end position="1020"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="1113"/>
            <end position="1137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="22">
        <location>
            <begin position="1138"/>
            <end position="1175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="1178"/>
            <end position="1196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="22">
        <location>
            <begin position="1199"/>
            <end position="1221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="1222"/>
            <end position="1233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="1330"/>
            <end position="1344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="1360"/>
            <end position="1369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="1380"/>
            <end position="1391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="1442"/>
            <end position="1462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="1490"/>
            <end position="1510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="1524"/>
            <end position="1534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="22">
        <location>
            <begin position="1557"/>
            <end position="1566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="22">
        <location>
            <begin position="1591"/>
            <end position="1605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="1626"/>
            <end position="1638"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="22">
        <location>
            <begin position="1672"/>
            <end position="1692"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00049522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS51848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="EMBL" id="KAJ8032600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1903" mass="216921" checksum="3FC2D2406299BE22" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BV85</accession>
    <name>A0A9Q1BV85_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_23709</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BV85"/>
    <dbReference type="OrthoDB" id="10524388at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040380924" evidence="1">
        <location>
            <begin position="21"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8033461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="310" mass="34662" checksum="E8398BE00E29E16C" modified="2023-09-13" version="1">MFRRVETIILLTLCTDAAVSVKILSSVTESTFEDGRFGYFAVPKERVEAKLAELHQQKSCHGARLTVPNRLILPEILEPDQHVVAIVYGKLGAKSIEDSLPINVKAKYETKADTVHFFIPFLTGPDSYAKPTYKLALGGFVSTTKPFIHPDIVIPTEQVDLLEVRPDGVTIVDAKGVLNTTWSQPCECEVPDEIILHDFEEYVDEFTLSFRSNPDFSFCDEPGAGGDNFCKYRNITDCACEFWTHGHTKKCEAENFSVTYITGKFKELSLFIDGPQDAVLTEINEGLYGIGAQEHCMEGEWALNRDHSVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BYN1</accession>
    <name>A0A9Q1BYN1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Sushi domain-containing protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_22566</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BYN1"/>
    <dbReference type="OrthoDB" id="6051552at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005533">
        <property type="entry name" value="AMOP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056619">
        <property type="entry name" value="C8-3_MUC4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051495">
        <property type="entry name" value="Epithelial_Barrier/Signaling"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003886">
        <property type="entry name" value="NIDO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001846">
        <property type="entry name" value="VWF_type-D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13802">
        <property type="entry name" value="MUCIN 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13802:SF52">
        <property type="entry name" value="MUCIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03782">
        <property type="entry name" value="AMOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23263">
        <property type="entry name" value="C8-3_MUC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06119">
        <property type="entry name" value="NIDO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00094">
        <property type="entry name" value="VWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00723">
        <property type="entry name" value="AMOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00539">
        <property type="entry name" value="NIDO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50856">
        <property type="entry name" value="AMOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51220">
        <property type="entry name" value="NIDO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51233">
        <property type="entry name" value="VWFD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0768">Sushi</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040194807" evidence="9">
        <location>
            <begin position="34"/>
            <end position="1211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1144"/>
            <end position="1166"/>
        </location>
    </feature>
    <feature type="domain" description="NIDO" evidence="12">
        <location>
            <begin position="123"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="AMOP" evidence="10">
        <location>
            <begin position="511"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="domain" description="VWFD" evidence="13">
        <location>
            <begin position="670"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="11">
        <location>
            <begin position="973"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="11">
        <location>
            <begin position="1045"/>
            <end position="1101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1173"/>
            <end position="1211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1173"/>
            <end position="1186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1198"/>
            <end position="1211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ8035366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1211" mass="134393" checksum="2F172D8D8F84865A" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C0G5</accession>
    <name>A0A9Q1C0G5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_20030</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C0G5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8036145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="72" mass="7831" checksum="4A9E78AD62119AAC" modified="2023-09-13" version="1">MSDSTGTHAVSLETKVAWDRVGLPPPYLFHINFSTFSKCGTNNEILEGKDKSDCDDSFAPSGSDDSQGERYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C118</accession>
    <name>A0A9Q1C118_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SCAN box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_17922</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C118"/>
    <dbReference type="OrthoDB" id="8188202at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF13">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCAN box" evidence="2">
        <location>
            <begin position="199"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="293"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="293"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="308"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="332"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8037173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="506" mass="57738" checksum="31954EE04B8D2960" modified="2023-09-13" version="1">MRKDEIKRYVLEYLVDESILPITVLETAITVPTDNTFELKRLEIEMNKEIRLKEMEREREREEREKEEREMQMQKEKEEREMQMQREKEAREHEFRLKQLELGVIKASDPKIGLDTGGFDVSKHVKFVPKFQEDNVEKFFNHFEKLGEQLKWPRDKWSILIQSNFTGKAQEVYSALSIEDSMDYDKVKKAILQAYELVPEAYRQKFRKYRKADTQTYVEFAYQKERHFDRWCASKKVSTFDTLRQLMLVEEFKRCVNDDIKTHLEENKADNLKEVANLADQYALTHKFGKVGQTQNKGQFSRPNKKYGNSSGTTGNSGSQSGSISPTNSGKKQPDSSRQKNDQVSSNKAAFKDISCGFCHKKGHTMANCFTLAKLKETETASNALANVENSSEVVVTLRPNSNDKIKEEYLPFVSEGFISLDGNSAHPPVKIKILRDTGATQSLLLDGVLPLSDSTSTGANVLIQGVECGFISVPLHKINLKSDLVSGSVIVGVRPTLPVKGYLCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C186</accession>
    <name>A0A9Q1C186_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Filamin-A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_20061</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the filamin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C186"/>
    <dbReference type="OrthoDB" id="10012602at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044801">
        <property type="entry name" value="Filamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017868">
        <property type="entry name" value="Filamin/ABP280_repeat-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001298">
        <property type="entry name" value="Filamin/ABP280_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38537:SF8">
        <property type="entry name" value="FILAMIN-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38537">
        <property type="entry name" value="JITTERBUG, ISOFORM N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00630">
        <property type="entry name" value="Filamin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00557">
        <property type="entry name" value="IG_FLMN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50194">
        <property type="entry name" value="FILAMIN_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="36"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="6">
        <location>
            <begin position="99"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="3">
        <location>
            <begin position="231"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="3">
        <location>
            <begin position="1110"/>
            <end position="1205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="369"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="406"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="653"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="757"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="823"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="369"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="669"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="698"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="839"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="872"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="894"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="920"/>
            <end position="948"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="952"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="970"/>
            <end position="980"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="994"/>
            <end position="1006"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8036171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1207" mass="134458" checksum="2FD86687CAE85ED2" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C230</accession>
    <name>A0A9Q1C230_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_17404</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8036770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C230"/>
    <dbReference type="OrthoDB" id="416119at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19446">
        <property type="entry name" value="REVERSE TRANSCRIPTASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8036770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="112" mass="12753" checksum="B78FD1D645210076" modified="2023-09-13" version="1">MLTEEEYWSVIQSMATGKVPGSDGLPLLEFYRVLWPQIKLFVVMSLNSGFLVQKICPEQSKAIIKLLLKEGKNPKKIGNWRPISLLYVDYKIAVSCISARLKLVMSFIIHPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C2Z8</accession>
    <name>A0A9Q1C2Z8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">E3 ubiquitin-protein ligase TRIM56</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_18086</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C2Z8"/>
    <dbReference type="OrthoDB" id="111250at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047153">
        <property type="entry name" value="TRIM45/56/19-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25462">
        <property type="entry name" value="BONUS, ISOFORM C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25462:SF296">
        <property type="entry name" value="MEIOTIC P26, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15227">
        <property type="entry name" value="zf-C3HC4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101898">
        <property type="entry name" value="NHL repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="22"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="331"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8037298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="816" mass="92231" checksum="B376679F605CA833" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C3W1</accession>
    <name>A0A9Q1C3W1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">18S rRNA aminocarboxypropyltransferase</fullName>
            <ecNumber evidence="6">2.5.1.157</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_18317</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Aminocarboxypropyltransferase that catalyzes the aminocarboxypropyl transfer on pseudouridine in 18S rRNA. It constitutes the last step in biosynthesis of the hypermodified N1-methyl-N3-(3-amino-3-carboxypropyl) pseudouridine (m1acp3-Psi).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>an N(1)-methylpseudouridine in rRNA + S-adenosyl-L-methionine = N(1)-methyl-N(3)-[(3S)-3-amino-3-carboxypropyl]pseudouridine in rRNA + S-methyl-5'-thioadenosine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:63296"/>
            <dbReference type="Rhea" id="RHEA-COMP:11634"/>
            <dbReference type="Rhea" id="RHEA-COMP:16310"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74890"/>
            <dbReference type="ChEBI" id="CHEBI:146234"/>
            <dbReference type="EC" id="2.5.1.157"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TDD superfamily. TSR3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.157" evidence="6"/>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C3W1"/>
    <dbReference type="OrthoDB" id="10262062at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106388">
        <property type="term" value="F:rRNA small subunit aminocarboxypropyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904047">
        <property type="term" value="F:S-adenosyl-L-methionine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000455">
        <property type="term" value="P:enzyme-directed rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01116">
        <property type="entry name" value="TSR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007209">
        <property type="entry name" value="RNaseL-inhib-like_metal-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022968">
        <property type="entry name" value="Tsr3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007177">
        <property type="entry name" value="Tsr3_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002621">
        <property type="entry name" value="PRK02287.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20426:SF0">
        <property type="entry name" value="18S RRNA AMINOCARBOXYPROPYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20426">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN TSR3 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04068">
        <property type="entry name" value="Fer4_RLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04034">
        <property type="entry name" value="Ribo_biogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3 6" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="5 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RNase L inhibitor RLI-like possible metal-binding" evidence="9">
        <location>
            <begin position="73"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="16S/18S rRNA aminocarboxypropyltransferase Tsr3 C-terminal" evidence="8">
        <location>
            <begin position="110"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="250"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="277"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="303"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8037491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="351" mass="39096" checksum="82F23F6579D9F37F" modified="2023-09-13" version="1">MGKKKGGHQKSHQTASSGIRGGGQKQTKHGRQHKFEKFSQDMCASLPDESSLSSRSATNSAEECEVPWKLPYPLAMWDLQHCDPKRCTGRKLCRKGLVKTLKLSQRFNGVILSPIGSQCVSPLDRSIVETSGIAVIDCSWAKLETTPFSKMQGPHPRLLPYLVAANPVNYGKPCKLSCVEAFAAACYIVGLEKDGYSMLRRFKWGPVFFELNSELLSIYASCSSSEDVVKAQDDWLEKLHHERDKEDLLDFDSDDDLEGVCNPNRQPVYDIPGSEESSSEEEEEEEEEEDGEDGQEDGSNFEKSGEESEDDHPKTPDDLEEDAKTLDPDCERSSDTEGASNKQRSPECEDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C3Z9</accession>
    <name>A0A9Q1C3Z9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ras-related protein Rab-14</fullName>
            <ecNumber evidence="7">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">HOLleu_16384</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="24">Nanhai2018</strain>
            <tissue evidence="24">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasmic vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Early endosome membrane</location>
            <topology evidence="4">Lipid-anchor</topology>
            <orientation evidence="4">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus</location>
            <location evidence="3">trans-Golgi network membrane</location>
            <topology evidence="3">Lipid-anchor</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Recycling endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="7"/>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C3Z9"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045335">
        <property type="term" value="C:phagocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055037">
        <property type="term" value="C:recycling endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006895">
        <property type="term" value="P:Golgi to endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090382">
        <property type="term" value="P:phagosome maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04122">
        <property type="entry name" value="Rab14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000344">
        <property type="entry name" value="Ras-related protein Rab-14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030702">
        <property type="entry name" value="Rab14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0007">Acetylation</keyword>
    <keyword evidence="21" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="12" id="KW-0967">Endosome</keyword>
    <keyword evidence="16" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="17" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="19" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="18" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0488">Methylation</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-0636">Prenylation</keyword>
    <keyword evidence="14" id="KW-0653">Protein transport</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="189"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="23">
        <location>
            <begin position="206"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00014884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="KAJ8038838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="215" mass="23908" checksum="24C6BCCEB69C21B9" modified="2023-09-13" version="1">MTSGPYNYSYIFKYIIIGDMGVGKSCLLHQFTEKKFMADCPHTIGVEFGTRIIEVSGQKIKLQIWDTAGQERFRAVTRSYYRGAAGALMVYDITRRSTYNHLSSWLTDARNLTNPNTVIFLIGNKSDLEAQRDVTYEEAKQFAEENGLLFLEASAKTGEHVEEAFLDTARKIYQNILDGSLDLNAAETGVQHKPSAPDSNQANLEPGGGKDSCSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C4V5</accession>
    <name>A0A9Q1C4V5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein STPG3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_16953</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C4V5"/>
    <dbReference type="OrthoDB" id="406368at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010736">
        <property type="entry name" value="SHIPPO-rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07004">
        <property type="entry name" value="SHIPPO-rpt"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="100"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="308"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="562"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="103"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="174"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="319"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="562"/>
            <end position="584"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8039288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="610" mass="68510" checksum="B025160B8E29CB86" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C7F6</accession>
    <name>A0A9Q1C7F6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Receptor-interacting serine/threonine-protein kinase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_17319</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C7F6"/>
    <dbReference type="OrthoDB" id="100767at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000488">
        <property type="entry name" value="Death_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016729">
        <property type="entry name" value="FADD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15077:SF9">
        <property type="entry name" value="C-TERMINAL OF ROC (COR) DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15077">
        <property type="entry name" value="FAS-ASSOCIATING DEATH DOMAIN-CONTAINING PROTEIN FADD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00531">
        <property type="entry name" value="Death"/>
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    </dbReference>
    <dbReference type="SMART" id="SM00005">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50017">
        <property type="entry name" value="DEATH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0675">Receptor</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="2">
        <location>
            <begin position="462"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="domain" description="Death" evidence="1">
        <location>
            <begin position="463"/>
            <end position="541"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8039560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="544" mass="62273" checksum="3E09BED503A17341" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CA91</accession>
    <name>A0A9Q1CA91_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Integrase core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_11740</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CA91"/>
    <dbReference type="OrthoDB" id="5971504at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058913">
        <property type="entry name" value="Integrase_dom_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46791:SF13">
        <property type="entry name" value="CLR5 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46791">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24764">
        <property type="entry name" value="rva_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase core" evidence="1">
        <location>
            <begin position="108"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF24764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8041048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="377" mass="44658" checksum="D1351A93C0C0A749" modified="2023-09-13" version="1">MQGYEAKEILYLLGLCHNIFISTRHLKRLLRRLNLRRRGQESPIEDIAMVMMNELEVNGRNIGYRSMWQRLNFAYGLHVTQNRTRQMLRIIDPEGVARRTANRLLRRIYRCPGPNHMVHIDGYDKLKPYGIAIHGAVDGFSRKILWLKAGYSNNNPRLIAKFYLDFIRHIDGVPKVIRADRGTEKVLVRRMHVALRWFNRDGYARENSFQYGRSSSNQRIESWWSQLRRTLTNSWINIFKDLRDRGLFDNSDSVQVECLRFCFVPLIQKQLDSITIQWNQHRIRKQRHGECPSGKPDLMYHIPEAYKTTDCKMSLDYPERELLEVEREYCSDFPRYGCHEGFVNVVEELVGDIDQYEMPNSPMEALRLYTSLLDLLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CAZ7</accession>
    <name>A0A9Q1CAZ7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_12903</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CAZ7"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013151">
        <property type="entry name" value="Immunoglobulin_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00047">
        <property type="entry name" value="ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040413073" description="Ig-like domain-containing protein" evidence="3">
        <location>
            <begin position="20"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="575"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="20"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="353"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8041957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="605" mass="67505" checksum="A3A0BDFE23F9652C" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CDL1</accession>
    <name>A0A9Q1CDL1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_00648</name>
        <name evidence="1" type="ORF">HOLleu_10403</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CDL1"/>
    <dbReference type="OrthoDB" id="8838209at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8043352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8048366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="70" mass="8206" checksum="1D26A911EADEE9CD" modified="2023-09-13" version="1">MLFGSFYCFNLEYPEGASITLEFIQRCLVKINPKRGTKSRGKKYYIIHPKVLTLVKQIKSFEADWDIESD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CFU7</accession>
    <name>A0A9Q1CFU7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_11267</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CFU7"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8043943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="115" mass="12197" checksum="ED9F22C6CEBBC394" modified="2023-09-13" version="1">MDHRLHNTSTDLQMAHGPQVASTSTDLQMVHGPQVASTSTDLQMVYGPQVASTSTDLQMVNGPQVASTSTDIQMVHGPQVASTSTDLQMVHGPQVASTSTDLQMVNGPQVASYQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CH84</accession>
    <name>A0A9Q1CH84_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Phosphoinositide phospholipase C</fullName>
            <ecNumber evidence="13">3.1.4.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HOLleu_08253</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Nanhai2018</strain>
            <tissue evidence="18">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + H2O = 1D-myo-inositol 1,4,5-trisphosphate + a 1,2-diacyl-sn-glycerol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:33179"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="ChEBI" id="CHEBI:203600"/>
            <dbReference type="EC" id="3.1.4.11"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MYST (SAS/MOZ) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.11" evidence="13"/>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CH84"/>
    <dbReference type="OrthoDB" id="269822at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004402">
        <property type="term" value="F:histone acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004435">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate phospholipase C activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046488">
        <property type="term" value="P:phosphatidylinositol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051209">
        <property type="term" value="P:release of sequestered calcium ion into cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00275">
        <property type="entry name" value="C2_PLC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000013">
        <property type="entry name" value="Histone acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.60:FF:000001">
        <property type="entry name" value="Histone acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.60">
        <property type="entry name" value="N-acetyl transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1230.10">
        <property type="entry name" value="Phospholipase C beta, distal C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002717">
        <property type="entry name" value="HAT_MYST-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001192">
        <property type="entry name" value="PI-PLC_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014815">
        <property type="entry name" value="PLC-beta_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042531">
        <property type="entry name" value="PLC-beta_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001711">
        <property type="entry name" value="PLipase_C_Pinositol-sp_Y"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025995">
        <property type="entry name" value="Tudor-knot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040706">
        <property type="entry name" value="Zf-MYST"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336:SF36">
        <property type="entry name" value="1-PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE PHOSPHODIESTERASE BETA-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336">
        <property type="entry name" value="PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE C FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00387">
        <property type="entry name" value="PI-PLC-Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08703">
        <property type="entry name" value="PLC-beta_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11717">
        <property type="entry name" value="Tudor-knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17772">
        <property type="entry name" value="zf-MYST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00390">
        <property type="entry name" value="PHPHLIPASEC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00149">
        <property type="entry name" value="PLCYc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69989">
        <property type="entry name" value="C-terminal domain of PLC-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51726">
        <property type="entry name" value="MYST_HAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50008">
        <property type="entry name" value="PIPLC_Y_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 13" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="9 13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MYST-type HAT" evidence="17">
        <location>
            <begin position="253"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="domain" description="PI-PLC Y-box" evidence="16">
        <location>
            <begin position="368"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="15">
        <location>
            <begin position="487"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="147"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="615"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="827"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="152"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="631"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="829"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="849"/>
            <end position="870"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ8045277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="998" mass="113416" checksum="BAF66C47C02FF190" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CIZ6</accession>
    <name>A0A9Q1CIZ6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Mirror-image polydactyly protein1 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_08593</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CIZ6"/>
    <dbReference type="OrthoDB" id="6426880at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026175">
        <property type="entry name" value="MIPOL1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22089">
        <property type="entry name" value="MIRROR-IMAGE POLYDACTYLY GENE 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22089:SF2">
        <property type="entry name" value="MIRROR-IMAGE POLYDACTYLY GENE 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="92"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="238"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="313"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="430"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="348"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="505"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="605"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="149"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="165"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="252"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="323"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="434"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8045560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="670" mass="75648" checksum="69F6A8F63A011266" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CJH6</accession>
    <name>A0A9Q1CJH6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ETS domain-containing protein Elk-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_05054</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the ETS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CJH6"/>
    <dbReference type="OrthoDB" id="10067219at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000418">
        <property type="entry name" value="Ets_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046328">
        <property type="entry name" value="ETS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849">
        <property type="entry name" value="ETS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849:SF133">
        <property type="entry name" value="ETS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00178">
        <property type="entry name" value="Ets"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00454">
        <property type="entry name" value="ETSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00413">
        <property type="entry name" value="ETS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00345">
        <property type="entry name" value="ETS_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00346">
        <property type="entry name" value="ETS_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50061">
        <property type="entry name" value="ETS_DOMAIN_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ETS" evidence="5">
        <location>
            <begin position="59"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="222"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="222"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8046398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="455" mass="49497" checksum="96CFF42782869A70" modified="2023-09-13" version="1">MDGSTPAPPVAHQHNQNGSWSGPEFKTEKPDQNMNESIPTRHLQNHIPLNAKRLESNNITLWQFLLDLLDDPNQSHLIVWTGHEGEFKLLNAEEVARMWGLRKNKTNMNYDKLSRALRYYYDKNIIKKVMGQKFVYKFVSYPDQMVYSSRVKVEMRGAPNSRPNSYPSTASVVAVKQEVSSGSAQNPLDRNTNREALEVKSEQSDERLVPLDCSVKSVSLAASSSTSDSSAPKYQIAVTRERSPVRSSPPPYPNRSIYVTESSSAYVTEARMHNEPKLVDSSARPIVASSAAKSFVYTTTTHTVGPRPKSANKPKPVQLTVPPIICATQQAASPGPSLSSSLVKHHPNSPSYLNTSSAVTALHTPALPATPIMVASPLLGQGGTPLLSQHGTPLVHLWGTFSPLPISPSVTNSNTFQFPTLVNGLASPMAVSGVPLQPLTPVLLSPPSSKAIIVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CL07</accession>
    <name>A0A9Q1CL07_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Mitochondrial carnitine/acylcarnitine carrier protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_05626</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CL07"/>
    <dbReference type="OrthoDB" id="14252at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015227">
        <property type="term" value="F:O-acyl-L-carnitine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902603">
        <property type="term" value="P:carnitine transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006839">
        <property type="term" value="P:mitochondrial transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050567">
        <property type="entry name" value="Mitochondrial_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624:SF4">
        <property type="entry name" value="CONGESTED-LIKE TRACHEA PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624">
        <property type="entry name" value="MITOCHONDRIAL BASIC AMINO ACIDS TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="67"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="5"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="100"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="201"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8046825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="294" mass="31709" checksum="8ADF43988E977C11" modified="2023-09-13" version="1">MTSNTSPVKNFLAGGFGGVCLVAAGHPLDTIKVRLQTQPTPPVYSGTFDCAKQIVMKESVFGLYKGMFAPILGVTPMYAVCFLGYGIGKKLQQPAANGEYGPAQLFAAGMVAGVFTTGIMAPGERIKCLLQIQASSTGPPKYAGPWDCAKQIYREAGLFRGLYRGTMATLLRDVPASGMYFMSYEMLKKAITPEGRTPDQLSPARILFAGGMAGIFNWMVAIAPDTLKSRYQTAPEGTYSGIRQVFVEMVRKEGFGALFKGLTPVMIRAFPANAACFLGFELAMKFLNWIAPNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CL82</accession>
    <name>A0A9Q1CL82_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_05174</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CL82"/>
    <dbReference type="OrthoDB" id="416710at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48312">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48312:SF1">
        <property type="entry name" value="SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8046489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="123" mass="14129" checksum="A5BDB0642A2DE0D8" modified="2023-09-13" version="1">MAAMTSSTSQPTVIFLWMVPRTQSTVLAKCMTFVDDTEVWLEPYLSCDFNDTLYDPNWKKGDPLAERMREAERKNEATEVMKSARKKVAEEESDFPNLIPQKDISVIRTKVITYVMDGRSTLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CLM1</accession>
    <name>A0A9Q1CLM1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Rho guanine nucleotide exchange factor 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_06610</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CLM1"/>
    <dbReference type="OrthoDB" id="1716625at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035025">
        <property type="term" value="P:positive regulation of Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051480">
        <property type="entry name" value="Endocytic_GEF_Adapter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001331">
        <property type="entry name" value="GDS_CDC24_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055251">
        <property type="entry name" value="SOS1_NGEF_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46006:SF8">
        <property type="entry name" value="DH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46006">
        <property type="entry name" value="RHO GUANINE NUCLEOTIDE EXCHANGE FACTOR AT 64C, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22697">
        <property type="entry name" value="SOS1_NGEF_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00741">
        <property type="entry name" value="DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="6">
        <location>
            <begin position="339"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="5">
        <location>
            <begin position="552"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="15"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="32"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="45"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="59"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="95"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="149"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="193"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8047576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="676" mass="77311" checksum="DAAB23BD7BE22607" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CMJ9</accession>
    <name>A0A9Q1CMJ9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_06430</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CMJ9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8047435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="229" mass="25822" checksum="AE03324DD31467A6" modified="2023-09-13" version="1">MDGDVVSNLSEVDVKLGMSGDRLNKAGKQRIKKGRYRNSPNWSNVCSDLSKLLGLNQILKISSTFGGFLLLWETGLALILLVLYAGAIGTMRDEERYCAVCVGITVNIISLLVFFIATSFAILDLGSKFSRADVMKGLKIVVHSVILMLQVSTFTVEFTDLVGSGEQECTIFFIFVECWVIFGNICMSEKEYAIASQQRLLADSRNRRKPQRRGERKHFYTRLHHYSIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CMN4</accession>
    <name>A0A9Q1CMN4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_06571</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CMN4"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="12"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8047543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="79" mass="8931" checksum="75E2B323225C77BA" modified="2023-09-13" version="1">MLSFLVEVKGHLGSQGSKSKISQESSHGFTRSQNANYPKNSQDHYLESLAIKMPNFPGISKIRYEPKIIFQHVKIALDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CMY2</accession>
    <name>A0A9Q1CMY2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_00362</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CMY2"/>
    <dbReference type="OrthoDB" id="416119at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09076">
        <property type="entry name" value="L1-EN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31635">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31635:SF196">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="494"/>
            <end position="756"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8048168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="971" mass="112448" checksum="A9FBB0C95E20D627" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CMY8</accession>
    <name>A0A9Q1CMY8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_00588</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CMY8"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8048322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="214" mass="24403" checksum="A45B3BD7430FBBBB" modified="2023-09-13" version="1">MAARSGKGYQRKALSSPDATRKETPQPKNKTKASSCAAEFEKLSTRSSRTSEKLKLERQLAIQKVELEFKERELQLQMEKLKMEKEMKKTKTLLDVVEESIGFESDEDEEKVKTMPILSEMPEDASNDNVERFFAAMPQSVTMAQEEEKKGTLRELVNSFRLPPIHVEKFKGDPLKYPVWLSTFDNLIASRASTPGEKLNLLSQHLERGATLHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CNI0</accession>
    <name>A0A9Q1CNI0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_01506</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CNI0"/>
    <dbReference type="OrthoDB" id="6157941at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912">
        <property type="entry name" value="IP13529P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912:SF34">
        <property type="entry name" value="NOTOCHORD-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8048977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="350" mass="39915" checksum="F8E5D458B977D274" modified="2023-09-13" version="1">MSLESKGVTVVSDGVSHTFKGTISFIAADNLGSHGMGGFMESFTSSRICRSCMCTYKSLKEPQLSLNFRERTIDGHRHQLGMIKQHPNLSSVYGVKSNCPFNKLCYFHVIKGLPFDPTHDLLESVCPLVWSKVLTSLVERKHLTVTQMNSKLQSFPFTGSDKSSKPTHVSWSSGRVVIKQNASQMWCFCRLAPVMFGGSVPKEDKIWDPVLQLNDVLNLSFAPRQNYQMVAYLNGLIEDFLHYFLMIFPDDGLTPKMHYLLHYSRQIEQFGPATNCSTIRFEAKHSYFKDLARKTKNRKNLAKTLAQRHQNLMSYYSCSEMWSGIDHITSTGFQVEDVNMFCPKIRDVLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CNN1</accession>
    <name>A0A9Q1CNN1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_01137</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CNN1"/>
    <dbReference type="OrthoDB" id="10522113at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8048712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="224" mass="25716" checksum="65C05F54B9553BA0" modified="2023-09-13" version="1">MLKLQKQRQDTQNVKISRKLSQSPIYVKGQLVYLYKPTSSSLTTNSRKTAAEWCGPFVIHEVLDRTHYVLATLKGDILSDVFNFNKLKPCFIRSAKQNITSVQKLTDALKQSDSNHVNMFSNESPIVEFHDEHVLPEVTRETVLCLSYTDPVCIQPYLEACEHNKGLAFPYLLKHEEYLKQFKLLSKAPKGDLEILRARYKLGQLQVLLSLDPGKKSSFWWNVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CPI8</accession>
    <name>A0A9Q1CPI8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_01116</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CPI8"/>
    <dbReference type="OrthoDB" id="6434680at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331:SF1">
        <property type="entry name" value="GAG-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13650">
        <property type="entry name" value="Asp_protease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8048696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="299" mass="33995" checksum="4804877953E25AEA" modified="2023-09-13" version="1">MPIVQVTVNNSCKVFTLLDSGSSNSFCSRALARRLGITGRSYEFELRNLNKSGTQKSEIVDLTLSPESGETLELFAVYMVDDIPIKSSPIETASYAHLSEILPMPAYKSDNVTVDLLTGQYNAEALVPLDVANQGSPTEMENKGLECSSWSQEDKLVKGLWDREHRKVDGHYELPIPWRDRSEPLPNNFIVAKSRLDNLYKKLVREDLYDRYNAQIVKLLEKGYAEEVPESELFAIDRTWDLPHHAVFLEEKPDKLRVVFDCVSTFKGKSLNDRCMQGPDMINKLLPVLLRFRQHSIAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CPQ5</accession>
    <name>A0A9Q1CPQ5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_01271</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CPQ5"/>
    <dbReference type="OrthoDB" id="7786610at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004748">
        <property type="term" value="F:ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009263">
        <property type="term" value="P:deoxyribonucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.395.10">
        <property type="entry name" value="Adenoviral Proteinase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000358">
        <property type="entry name" value="RNR_small_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23409:SF21">
        <property type="entry name" value="CAPSID PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23409">
        <property type="entry name" value="RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE SMALL CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="484"/>
            <end position="510"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8048805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="510" mass="57339" checksum="9144CC1892E0199D" modified="2023-09-13" version="1">MDVKHFHNMFVYCDIVDPHAVGHAKVPLIRVVTVKRGSKTVDMIGPVHSDIFFQDKYLLNGVNMKLKLIRSKDQFCLMSDDADVDYKVSITDASLFVRKVKLNPSVSLAHARALEKATAKYPISRVVTKMFSIPTGSMSFVQDHIFLGQLPKKIIVGCVRNTAFNGSYTENPCNFEHFGTNYLAVYLNGEQVPYKPLKPKYDAAGGENYIRAYHTLFSGTDKAQEDTGNAISREDYHRGYTLYAFDLTPDLSSGGHFNLVKQGNLRMELQFARPLDTTVNVVIYAELDNIIEVDKARNIVFDYSSLKSVKLRYPCALVVNTDPSYLPGEHWVAIFLNASRHGEYFDSYGLPPSIYPDIAHFLIRNCESFRYNSHLLQGLGSTVCGQYVLFYLLHRCRGYTLDEITGWFSPNLDTNDEQVQGFINKHFPCVDCGGAHLRHFICATSVRSQKKLSRNVSLRADSTIINDRNGRTWKGFGKGGYYGENKGKKGNTQGAVGRLKGVNKNKNGLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CQI3</accession>
    <name>A0A9Q1CQI3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_01861</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CQI3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040489602" evidence="1">
        <location>
            <begin position="25"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8049221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="119" mass="13965" checksum="2D99C806DD0EB210" modified="2023-09-13" version="1">MPFAYFVRYWGGFLLLGFFPSLFSNSVPQSWRPTKAYCQMRETLRIMSTVGDVSQSSAGDQYRAIFDDLVGQVQPNDYASGPEVWRVVHRTLDHRRRDLLWRIAHDAIVTNLKRLRWRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CQL3</accession>
    <name>A0A9Q1CQL3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcriptional coactivator p15 (PC4) C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_01270</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CQL3"/>
    <dbReference type="OrthoDB" id="2505440at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.31.10">
        <property type="entry name" value="Transcriptional Coactivator Pc4, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003173">
        <property type="entry name" value="PC4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009044">
        <property type="entry name" value="ssDNA-bd_transcriptional_reg"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02229">
        <property type="entry name" value="PC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54447">
        <property type="entry name" value="ssDNA-binding transcriptional regulator domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcriptional coactivator p15 (PC4) C-terminal" evidence="1">
        <location>
            <begin position="14"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8048804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="234" mass="27138" checksum="BE0B08850364896F" modified="2023-09-13" version="1">MNEVVPNDSKKRFHLGGSYFVVLQRYRGSVFVCIREYHYDEHHIPVLAGKRGINLRIPEWNKLYEHSEQITAAVEKLVDQIDLSKSGITAVKAEQDLVEFIFAAMVDTVIYKWFQIHCEGCQEHYPSQRDHACLNYGSMDSLIGLYFREFHLLVCTDWVVGACQTYLKHTQYHVIVSMVEDLRKQWVNSPQLAADAIENSSLKKTVRDDIETDVVSKWLGDVKFHDHIELSYSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CRU0</accession>
    <name>A0A9Q1CRU0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NLR family CARD domain-containing protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_02812</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CRU0"/>
    <dbReference type="OrthoDB" id="8441676at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312">
        <property type="entry name" value="NACHT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312:SF2">
        <property type="entry name" value="NUCLEOTIDE-BINDING OLIGOMERIZATION DOMAIN-CONTAINING PROTEIN 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NACHT" evidence="1">
        <location>
            <begin position="67"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8049881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="570" mass="65620" checksum="58809968314F31EE" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CSF3</accession>
    <name>A0A9Q1CSF3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">NLR family CARD domain-containing protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_04048</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CSF3"/>
    <dbReference type="OrthoDB" id="427518at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312">
        <property type="entry name" value="NACHT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312:SF2">
        <property type="entry name" value="NUCLEOTIDE-BINDING OLIGOMERIZATION DOMAIN-CONTAINING PROTEIN 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040121264" evidence="2">
        <location>
            <begin position="23"/>
            <end position="1185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="481"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="domain" description="NACHT" evidence="3">
        <location>
            <begin position="611"/>
            <end position="736"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8050737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1185" mass="135945" checksum="2C520A5EC47130B9" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CTV8</accession>
    <name>A0A9Q1CTV8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_03826</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10025024at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01644">
        <property type="entry name" value="RT_pepA17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005312">
        <property type="entry name" value="DUF1759"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040676">
        <property type="entry name" value="DUF5641"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008042">
        <property type="entry name" value="Retrotrans_Pao"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331:SF5">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03564">
        <property type="entry name" value="DUF1759"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18701">
        <property type="entry name" value="DUF5641"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05380">
        <property type="entry name" value="Peptidase_A17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="2">
        <location>
            <begin position="1742"/>
            <end position="1935"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="660"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="660"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="671"/>
            <end position="703"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8050574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="2119" mass="239587" checksum="FED8E250F70C3B04" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1GWU1</accession>
    <name>A0A9Q1GWU1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_30427</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1GWU1"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8028242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="136" mass="16542" checksum="6B6CE076192A5FAF" modified="2023-09-13" version="1">MPFNLDKFVASPSVEELDSLKKSEIVKVAKRYGIEFQPLMRKDEIKRYVLEYLVDESILPITVLETAITVPTDNTFELKRLEIEMNKEIRLKEMEREREKEEREMQMQKEKEEREMQMQKEKEEREMLGYWGIRCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1GX32</accession>
    <name>A0A9Q1GX32_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_30611</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1GX32"/>
    <dbReference type="OrthoDB" id="10072337at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040257906" evidence="1">
        <location>
            <begin position="23"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8028392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="228" mass="25101" checksum="5F18B385CF8F7E92" modified="2023-09-13" version="1">MNLPEILVTFLFGMCGITITSSQSSGDSTKLRCYACISEEPCSSDDVGIQQCDPLAGLPCVSSNLTYTFQLDSGEKKITSISTGCLRSEVDFTPPTRNGCIDGSTYLDLVLIQPPPGIKINLPTADGKVCFCETDLCNTQTILPASLRNCPDGFPPVSCDVNPCEKAECPLNPHAVCIVNYCNRQHHCLAEWFHGERNRVDCGETYIELVHHQYIWLTSKLCCTNFHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1GYM6</accession>
    <name>A0A9Q1GYM6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_28725</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1GYM6"/>
    <dbReference type="OrthoDB" id="6111086at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8029352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="101" mass="11814" checksum="B915C64DB2D8525F" modified="2023-09-13" version="1">MVLKKDDLRVVCEVWCSFKNLTGKDNHTMDQFLNEYEKKVKALQRTELPSIPEVVMAMQLTDGAGLDKKDNQIVLTVVDYKKKEEMYDQMNQNAKNALRFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H0J7</accession>
    <name>A0A9Q1H0J7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">SET domain-containing protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_27056</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H0J7"/>
    <dbReference type="OrthoDB" id="341421at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016279">
        <property type="term" value="F:protein-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19177">
        <property type="entry name" value="SET_SETD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1410.10:FF:000002">
        <property type="entry name" value="SET domain-containing protein 4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1410.10">
        <property type="entry name" value="set domain protein methyltransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050600">
        <property type="entry name" value="SETD3_SETD6_MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044429">
        <property type="entry name" value="SETD4_SET"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271:SF151">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271">
        <property type="entry name" value="UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SET" evidence="1">
        <location>
            <begin position="44"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8030597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="441" mass="51073" checksum="730E433A990F6447" modified="2023-09-13" version="1">MAATPHGQAQNCKGRRYRRKQRKLVNSENISHERKHIELQQWLSQNGVKYHHLRPANFKTTGRGLMTVKAYKDGDVIISIPKKLLITTQVALESELKDVIVRFPKKLTARLIICAFLIIEKMKGSSSFWAPYIRCLPESFDTPAYFTEEELSLLPDSLQVKSKEQRVKIQQEFRKLKQFFRFAKEFNICKGEELSLSFFKWAWFVVNTRSLYMEESYSAGRYLDPSEKDVYVLAPFLDLLNHSPEVDVTGAYNPSNDCYEISTVTPFHRYDQVFIYYGPHDNKTLLLDYGFVVPSNPHSVVQISLDDVVKMSAGHMESTFPEDYAIEERQKVITENHLWDFSCSLNGLSWHLAICLDILCLDPNQFGREEWKSIVAGHETPDNCKDRSTRVAKNVLKQYLEETSKKCQEISAVGHQTDHILMVLQLFHEEEEILTNCLSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H0Z3</accession>
    <name>A0A9Q1H0Z3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_30254</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H0Z3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8028111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="231" mass="26061" checksum="F324C5463373892B" modified="2023-09-13" version="1">MSETDIKGDVVADEGSQAQVDDQASGTKPIRQRTLTEKGQQYQIEQGEHKFRNLISTWRRRASEIECLLSEQGDISQVKGLRDIAMSVMAEITSVYFKLDQLLLNVGLMGSKYKAYEEVENEHYALMKRVSEFIRELDESKSLKDSRSSKSHLSKSSKSSFSIKVDAEIKAASLEAKLKYIEVKAKYKTELERIKFEIAQAKLGAVEKVERQSLTNLGSEVSGGKRQITEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H1I0</accession>
    <name>A0A9Q1H1I0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cytoskeleton-associated protein 2-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_30878</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H1I0"/>
    <dbReference type="OrthoDB" id="6288182at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015630">
        <property type="term" value="C:microtubule cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007026">
        <property type="term" value="P:negative regulation of microtubule depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026165">
        <property type="entry name" value="CKAP2_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16076">
        <property type="entry name" value="CYTOSKELETON ASSOCIATED PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16076:SF8">
        <property type="entry name" value="CYTOSKELETON-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="113"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="165"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="367"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="165"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="188"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="216"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="381"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8028591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="509" mass="57752" checksum="F58DD9CCBBBF893B" modified="2023-09-13" version="1">MKTVKGQSECPEKHPSQRLDGCRSPSMWEKLAKWKAEKENLKRNSSVKTSSRQPFKVGRTQMRRGKVPARASEKIKLLVESNLNTNWTYRTSPRYSSCRKSVSHVKKSLCEKKLSSSQSVRKPHQARRETITLSAGDNTFSDKNICSHLNFKENDEKDIKVVPKERQKLQDRKLQSISKQKSSKVTKPRSSLQSKDIRSSNNTSSQKTLTNSKQKAQREGKLQKKKVEDKSVSVPAKDQTVSVSEEQTKLRLKLEQWLLAKGRTPSRATRFMKTPRAVCKVKQRVHDETHSSSYSNVLSVDEAMDACKDLLKEGCPPEYLSDWLDGLSAKLPCLQEVESFVSCRNLVLFAANDVAMTTHSPYLTSNSVEHQEEDSADAKKPASHQRRKSRRSSYQLLSPCEEVEESSVLKYQVREATPYFKRIHQALGQTSSTPSTMIVTPVRRSMRLSKRKSVSFASRQNNQLDTCVDSLNELPCGQEVQVVLRANRALEEEFGLVDEEPGQCITNNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H1Q7</accession>
    <name>A0A9Q1H1Q7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Myogenesis-regulating glycosidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_28798</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H1Q7"/>
    <dbReference type="OrthoDB" id="10070917at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06592">
        <property type="entry name" value="GH31_NET37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050985">
        <property type="entry name" value="Alpha-glycosidase_related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048395">
        <property type="entry name" value="Glyco_hydro_31_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000322">
        <property type="entry name" value="Glyco_hydro_31_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43053">
        <property type="entry name" value="GLYCOSIDASE FAMILY 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43053:SF4">
        <property type="entry name" value="MYOGENESIS-REGULATING GLYCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01055">
        <property type="entry name" value="Glyco_hydro_31_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21365">
        <property type="entry name" value="Glyco_hydro_31_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 31 TIM barrel" evidence="7">
        <location>
            <begin position="385"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 31 C-terminal" evidence="8">
        <location>
            <begin position="638"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="46"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="48"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF21365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8029415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="722" mass="81177" checksum="0F97B2BF9297BB66" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H2N1</accession>
    <name>A0A9Q1H2N1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_23086</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8032982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H2N1"/>
    <dbReference type="OrthoDB" id="6495350at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF10">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="245"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="726"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="779"/>
            <end position="792"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8032982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="792" mass="88822" checksum="57C7080594D3958B" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H3V9</accession>
    <name>A0A9Q1H3V9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_21230</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H3V9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8034407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="133" mass="16134" checksum="AB4D1116981230F2" modified="2023-09-13" version="1">MPFNLDKFVASPSVEELDSLKKSEIVKVAKHYGIEFQPLMRKDEIKRYVLEYLVDESILPSTVLETAITVPTDITFKLKRLEIEMNKEIRLKEMEREMKKEKEEREIQMQKEKEEREMQMQREKEAREHEFRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H4L9</accession>
    <name>A0A9Q1H4L9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Otoferlin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_23278</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H4L9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007009">
        <property type="term" value="P:plasma membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037721">
        <property type="entry name" value="Ferlin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032362">
        <property type="entry name" value="Ferlin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12546">
        <property type="entry name" value="FER-1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12546:SF60">
        <property type="entry name" value="MISFIRE, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16165">
        <property type="entry name" value="Ferlin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="70"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="Ferlin C-terminal" evidence="7">
        <location>
            <begin position="37"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF16165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8033134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="102" mass="11638" checksum="0E21B373549B679B" modified="2023-09-13" version="1">MARKPFQEKITTYDIGVRDFPDLGVFSASKLILLVPLPLSPSSRPDSSVGWFTNPLKALYYGLWIQYRWLLLKLLIAVVIVAFFALFIYNLPGYTVKWVLGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H5E8</accession>
    <name>A0A9Q1H5E8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ficolin-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_24333</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H5E8"/>
    <dbReference type="OrthoDB" id="7871457at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050373">
        <property type="entry name" value="Fibrinogen_C-term_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143:SF458">
        <property type="entry name" value="FIBRINOGEN C-TERMINAL DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143">
        <property type="entry name" value="FIBRINOGEN/TENASCIN/ANGIOPOEITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00186">
        <property type="entry name" value="FBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8033944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="84" mass="9508" checksum="624D1943E9CD1BAF" modified="2023-09-13" version="1">MDGSQDFFLYWSDYAHGFGTPDRELWLGNDKLHSLTTQRNYQLRVDVVDKHGAPYYAKYSSFRVSGASEKYELSVGGYSGNAGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H984</accession>
    <name>A0A9Q1H984_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Beta-2 adrenergic receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_14864</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H984"/>
    <dbReference type="OrthoDB" id="10011262at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00637">
        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22752">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="20"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="58"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="92"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="135"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="182"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="271"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="308"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="37"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="219"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="219"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="232"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8040542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="374" mass="42330" checksum="61EC8096CFD95592" modified="2023-09-13" version="1">MNSTKVTVLNGIEEVTHRLLLSTLFTAGPVIFLGSFGNICVIVVFIRKKGLRTTSISLTVALMFFSLNLIGCAYPVQCLLDLFFFTSQPWCYMIGSILPTASVQVTVCLFVLTVERYIAIVHPFMAGNLFTTKRVTLIAAFIALYSYSFSYGIGALVWIGKGELWRQTRRCNWLFVTPSKNLILATMLHWMILIPIMVILFAHLFITVRRHIRSISSVQPATSQAPSQPVLSSPTNSRNENTRSNNHSALRISETLRRRTNRRMLREAKSALRLFFVLVIYICNWVPFTIFVVTGVLSEENVSPMQYVLLHTSVYSAGAIAPYIYGFGNRRIFREIKVMILPRSCTTLADDSGNTVHFISTRTTRQDTREIDED</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HCD0</accession>
    <name>A0A9Q1HCD0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Nuclear factor interleukin-3-regulated protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_14295</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bZIP family. NFIL3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HCD0"/>
    <dbReference type="OrthoDB" id="6151507at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007623">
        <property type="term" value="P:circadian rhythm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14694">
        <property type="entry name" value="bZIP_NFIL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000025">
        <property type="entry name" value="nuclear factor interleukin-3-regulated protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047229">
        <property type="entry name" value="NFIL3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047106">
        <property type="entry name" value="NFIL3-like_bZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15284:SF0">
        <property type="entry name" value="GH23983P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15284">
        <property type="entry name" value="NUCLEAR FACTOR INTERLEUKIN-3-REGULATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07716">
        <property type="entry name" value="bZIP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="8">
        <location>
            <begin position="128"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="296"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="373"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="484"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="301"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="376"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8040096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="520" mass="58343" checksum="0D82294DD47310B9" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HCF4</accession>
    <name>A0A9Q1HCF4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SCAN box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_15410</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HCF4"/>
    <dbReference type="OrthoDB" id="6077919at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF1">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8040949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="106" mass="12543" checksum="9151971599A2702D" modified="2023-09-13" version="1">MSGKAFKVYSRIPSDEAKNYKKLKVALLQRYNLTEDGFRDKLRNSRIKPGETYVQFGERLRGYLNRWVETANLQKNFDDLSDLIIREQIVSHCGRDMSIFLKERQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HFE2</accession>
    <name>A0A9Q1HFE2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Hydantoinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_07117</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HFE2"/>
    <dbReference type="OrthoDB" id="5404895at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.390.10:FF:000003">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.390.10">
        <property type="entry name" value="CV3147-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1610.10">
        <property type="entry name" value="CV3147-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008040">
        <property type="entry name" value="Hydant_A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002821">
        <property type="entry name" value="Hydantoinase_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045079">
        <property type="entry name" value="Oxoprolinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048350">
        <property type="entry name" value="S-Me-THD-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024071">
        <property type="entry name" value="S-Me-THD_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010318">
        <property type="entry name" value="S-Me-THD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027479">
        <property type="entry name" value="S-Me-THD_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365">
        <property type="entry name" value="5-OXOPROLINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365:SF10">
        <property type="entry name" value="HYDANTOINASE_OXOPROLINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05378">
        <property type="entry name" value="Hydant_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01968">
        <property type="entry name" value="Hydantoinase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20906">
        <property type="entry name" value="S-Me-THD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06032">
        <property type="entry name" value="S-Me-THD_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160991">
        <property type="entry name" value="CV3147-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydantoinase/oxoprolinase N-terminal" evidence="2">
        <location>
            <begin position="6"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="Hydantoinase A/oxoprolinase" evidence="1">
        <location>
            <begin position="194"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="S-Me-THD N-terminal" evidence="3">
        <location>
            <begin position="583"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="domain" description="S-Me-THD N-terminal" evidence="3">
        <location>
            <begin position="703"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="domain" description="S-Me-THD-like C-terminal" evidence="4">
        <location>
            <begin position="787"/>
            <end position="978"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF06032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF20906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8044384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="991" mass="107029" checksum="970990E00D3CB163" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HFG7</accession>
    <name>A0A9Q1HFG7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Nuclear nucleic acid-binding protein C1D</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_07216</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Plays a role in the recruitment of the exosome to pre-rRNA to mediate the 3'-5' end processing of the 5.8S rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Monomer and homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
            <location evidence="7">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the C1D family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HFG7"/>
    <dbReference type="OrthoDB" id="1421013at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000178">
        <property type="term" value="C:exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000460">
        <property type="term" value="P:maturation of 5.8S rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011082">
        <property type="entry name" value="Exosome-assoc_fac/DNA_repair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007146">
        <property type="entry name" value="Sas10/Utp3/C1D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15341:SF3">
        <property type="entry name" value="NUCLEAR NUCLEIC ACID-BINDING PROTEIN C1D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15341">
        <property type="entry name" value="SUN-COR STEROID HORMONE RECEPTOR CO-REPRESSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04000">
        <property type="entry name" value="Sas10_Utp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 7" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="123"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU368003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8044459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="147" mass="16897" checksum="76A8E8792D0E3E52" modified="2023-09-13" version="1">MAEKKSKSCPPEIQPSVDRLRKSLDDVEKILEPFLNGSILDVQDKMTCLEKAKVELVSLYAINSLFWMYLCTQGVDTKEHPIKHELARIQQYMRRMKQITDREKAAKLDKSAAKRFINSALWDRNQKGETSKKRKATSDDGGVPNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HGA8</accession>
    <name>A0A9Q1HGA8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_11209</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HGA8"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8043903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="85" mass="9623" checksum="C9B83F9FBDDD7B8A" modified="2023-09-13" version="1">MTLATCNAVTSVKHVKVNNCTQKISQGERHRPYAYDVTTHRIESALSTELSAWHNTTLSTQMQTADILRETASSCVQYVHHMYLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HGJ8</accession>
    <name>A0A9Q1HGJ8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Apoptosis-inducing factor 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_11597</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAD-dependent oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8044206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HGJ8"/>
    <dbReference type="OrthoDB" id="432169at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="entry name" value="Rieske [2Fe-2S] iron-sulphur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050446">
        <property type="entry name" value="FAD-oxidoreductase/Apoptosis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028202">
        <property type="entry name" value="Reductase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43557">
        <property type="entry name" value="APOPTOSIS-INDUCING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43557:SF2">
        <property type="entry name" value="RIESKE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14759">
        <property type="entry name" value="Reductase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00355">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00469">
        <property type="entry name" value="PNDRDTASEII"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="FAD/NAD-linked reductases, dimerisation (C-terminal) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="ISP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="6" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rieske" evidence="10">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8044206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="473" mass="51549" checksum="9306235725EB0045" modified="2023-09-13" version="1">MERVLSNGRVRCPWHGACFNTKTGDIEDFPGVDSIPCFKVRVEGNDVIVQAQKKQLQSHKRIKKMSLKSADDDKVFLIIGGGGAGLMCAETLRQEGFGGRIVIATKEKYLPYDRPKLSKAMESSAESIQLRKEDFFQVYDIEVKVEHEAVSVDTELNSVKFQNGDALSYEKLLIATGGLPRKLDIPGNDLKNICSLRSPDDANYIALSGKERNVVVVGSSFIGMEVASFFTGKARSVTVIGRGPVPYATSLGEQIGGAVLKLAESKGVKFHMNCSVREFKGENGVLSSIVLTTGENIAADLCVLGVGVNPCTDFIQGSGVQLSSKNYVVVNKHLCTNNSDVFAAGDIVEFPLHYNNDLAVNIGHWQIAHYHGRIAAFNMVGHEVEVETVPFFWTAVFGKNIRFSGYNHGFDDVIISGSLVELKFIAYYARGDYVVAVATLNSDPVASKFAEFLASGKRLKKSQVMSAEPVLAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HGM1</accession>
    <name>A0A9Q1HGM1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Delta-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HOLleu_08861</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Nanhai2018</strain>
            <tissue evidence="17">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Putative Notch ligand involved in the mediation of Notch signaling.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Membrane</location>
            <topology evidence="1 13">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HGM1"/>
    <dbReference type="OrthoDB" id="283575at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000061">
        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000064">
        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.140:FF:000001">
        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000173">
        <property type="entry name" value="Neurogenic locus notch protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000122">
        <property type="entry name" value="Protein crumbs homolog 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000118">
        <property type="entry name" value="protein delta homolog 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.3510">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001774">
        <property type="entry name" value="DSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013032">
        <property type="entry name" value="EGF-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051355">
        <property type="entry name" value="Notch/Slit_guidance"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011651">
        <property type="entry name" value="Notch_ligand_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45836:SF23">
        <property type="entry name" value="NEUROGENIC LOCUS NOTCH HOMOLOG PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45836">
        <property type="entry name" value="SLIT HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01414">
        <property type="entry name" value="DSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21700">
        <property type="entry name" value="EGF_DL_JAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12661">
        <property type="entry name" value="hEGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07657">
        <property type="entry name" value="MNNL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00010">
        <property type="entry name" value="EGFBLOOD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00051">
        <property type="entry name" value="DSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51051">
        <property type="entry name" value="DSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 13" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="9 11 13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 11 13" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 13 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <keyword evidence="4 13 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="608"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="domain" description="DSL" evidence="16">
        <location>
            <begin position="189"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="15">
        <location>
            <begin position="298"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="15">
        <location>
            <begin position="374"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="15">
        <location>
            <begin position="415"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="15">
        <location>
            <begin position="452"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="15">
        <location>
            <begin position="489"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="15">
        <location>
            <begin position="526"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="191"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="204"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="224"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="326"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="404"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="441"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="477"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="515"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="554"/>
            <end position="563"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU280815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ8045779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="827" mass="91437" checksum="47CB8D9E5A99C433" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HJR7</accession>
    <name>A0A9Q1HJR7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_02443</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HJR7"/>
    <dbReference type="OrthoDB" id="416987at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22955:SF77">
        <property type="entry name" value="ASPARTIC PUTATIVE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22955">
        <property type="entry name" value="RETROTRANSPOSON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8049619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="192" mass="21026" checksum="48EB06AA3AA647DE" modified="2023-09-13" version="1">MSHLVALTVPLDTFSLQNCVHESRSNQDQVQGTGDLSQRSTALSGTEGAFHASAQTQSQRLAAPLPDGTDIPEDSFKKFCNLESIGICAEAESSNNSADPVLERFRETIRHSEVENRPVIRESSLTTKIPPVFDASAKGYNGLYLNDCLETGPSLIPNLTGILLRFWRWQIGLTADITKAFLQIKISFGSGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HK61</accession>
    <name>A0A9Q1HK61_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF4371 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_03272</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HK61"/>
    <dbReference type="OrthoDB" id="6159421at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025398">
        <property type="entry name" value="DUF4371"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46880:SF5">
        <property type="entry name" value="DUF4371 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46880">
        <property type="entry name" value="RAS-ASSOCIATING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14291">
        <property type="entry name" value="DUF4371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4371" evidence="1">
        <location>
            <begin position="43"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8050174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="429" mass="48862" checksum="4D481753BC50896B" modified="2023-09-13" version="1">MTKVFASLYFLCKQRIAHTTNYEPLLDFIGFLGDKLKEKVFVGRNAHYTSRKSIQEYLKCLSDVLEKDILEELKSSQQFSLMFDETTDCSVREQMVLRARYIHSVNGNIEVRYLKIIDCLTDRHTLDAETISSIVVKYIEDHDRSYKHLKGIGTDGAAVMTGRSNGAVKRIRQKQLSAQNDEEFKCEAVGCHCAAHKLNLASQQAGDHISYIKKFKEILRQLYDFFDNSAVRSAGLLAMQKLLGCPERKVLEPSSTRWLSIGNAVLRLKDILPSVVCSLEREAEERGNAVAAGLCRFLSEYEFACTLLLLCDVLPKVNVLSRVFQNCSVDFSTAMQLKQSIVSMLESLKSVEGENLKALDFFILQMENAGITIKYQRGLRQDCSSREEAKTRFMRNIKGPFLKSFLQTLMIDLQMVMYWVRSAFSIPPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HKJ8</accession>
    <name>A0A9Q1HKJ8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PiggyBac transposable element-derived protein 4 C-terminal zinc-ribbon domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_04001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HKJ8"/>
    <dbReference type="OrthoDB" id="6774960at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47272">
        <property type="entry name" value="DDE_TNP_1_7 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47272:SF1">
        <property type="entry name" value="PIGGYBAC TRANSPOSABLE ELEMENT-DERIVED PROTEIN 3-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8050699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="119" mass="13595" checksum="A06C0F1D70FA531E" modified="2023-09-13" version="1">MAVVNAWFVYKRDCIELASSKPMPLRKFQASVAAALCSSGKPARGRPSLESAKKRKRQVNPAPVEDVRFDGVRHLPTYEGKRQRCRNCPDGFAFVKCVKCKVHLCLNKNRNCFLEYHMH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0Y9Z2</accession>
    <name>A0A9Q0Y9Z2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Kinesin-like protein KIN-4A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_43060</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000197">
        <property type="protein sequence ID" value="KAJ8018773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0Y9Z2"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005875">
        <property type="term" value="C:microtubule associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051231">
        <property type="term" value="P:spindle elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969">
        <property type="entry name" value="CHROMOSOME-ASSOCIATED KINESIN KIF4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969:SF15">
        <property type="entry name" value="CHROMOSOME-ASSOCIATED KINESIN KIF4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="9">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="13"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8018773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="87" mass="9846" checksum="3AA4FDC77D5D216C" modified="2023-09-13" version="1">MALLKKGSDGRATGSTGMNKTSSRSHAIFTINIEQISRDDRLVNFISIEIEEVELGATFLLDSFLPKICYERQVLYIHIKGSWSRQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YD54</accession>
    <name>A0A9Q0YD54_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NACHT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_42131</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000034">
        <property type="protein sequence ID" value="KAJ8019331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YD54"/>
    <dbReference type="OrthoDB" id="120976at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312">
        <property type="entry name" value="NACHT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312:SF2">
        <property type="entry name" value="NUCLEOTIDE-BINDING OLIGOMERIZATION DOMAIN-CONTAINING PROTEIN 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NACHT" evidence="2">
        <location>
            <begin position="134"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8019331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="719" mass="83700" checksum="AB849CCB233EA583" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YDA2</accession>
    <name>A0A9Q0YDA2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glutamyl-tRNA(Gln) amidotransferase subunit A, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_39708</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the amidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YDA2"/>
    <dbReference type="OrthoDB" id="421993at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000120">
        <property type="entry name" value="Amidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020556">
        <property type="entry name" value="Amidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005565">
        <property type="entry name" value="PRK07235.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895:SF170">
        <property type="entry name" value="AMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895">
        <property type="entry name" value="TRANSAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00571">
        <property type="entry name" value="AMIDASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidase" evidence="2">
        <location>
            <begin position="95"/>
            <end position="508"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8020185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="522" mass="56315" checksum="6CE841C122623D78" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YE67</accession>
    <name>A0A9Q0YE67_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Octopamine receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_40964</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8021172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YE67"/>
    <dbReference type="OrthoDB" id="9442282at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00637">
        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24238:SF47">
        <property type="entry name" value="ECDYSTEROIDS_DOPAMINE RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24238">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="45"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="122"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="178"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="355"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="388"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="10">
        <location>
            <begin position="26"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="206"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="216"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="238"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8021172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="434" mass="50015" checksum="A0DFAFDE25AE9042" modified="2023-09-13" version="1">MSETKFLYTWVVITYRSIVTLTGILGNLLILAVYWNKRSLGSAHVFISTLALCDLCSCLIIALEIHYWLNELNYTNTFLCKLFLTWERIGIDFSVCITAAIAWDRYLAVSQPINGRWTRKKAVKICLVCTVVVVVFTAAIPFLGYIEPTAIQADTGIVNGTRCEVCTKDLVSYLLGCASHYILFFLMFVATIFLYVKLWNRMNKSQDTTRMKKRRDTTSEEKEPRRDRNRGNKKYARSSKEVLEEGRDNHYLKKSSEKEVTKKKSIDTKSFNRVVPPGHSTIKTVKETKFMLSSPSSGPDMTLEGGQAIRQEAKYPAIIHNTKVNHHDRTRTIKYGNTKSQVPNRPRPISRLTKMLILATLVFVCTWSLSLVLYMTYPVWERIPRQSFAFTIIMVLVLTGGINHAINPVLYSFMNPRFRTECKMMLRRLRRRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YEE2</accession>
    <name>A0A9Q0YEE2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Integrase core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_42924</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000165">
        <property type="protein sequence ID" value="KAJ8018854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YEE2"/>
    <dbReference type="OrthoDB" id="8956331at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058913">
        <property type="entry name" value="Integrase_dom_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46791:SF5">
        <property type="entry name" value="CLR5 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46791">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24764">
        <property type="entry name" value="rva_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase core" evidence="1">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF24764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8018854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="97" mass="11260" checksum="2A50D77AF0EA98F3" modified="2023-09-13" version="1">MIVYLTCSTTNQADVVQRSFMQASKRYGLPSRVRSDYGSENIDVALLMNLLRGSGRGSHITGQSVHNERIERLWRDVHKDVTSTFYEEFYKLEDRDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YFW2</accession>
    <name>A0A9Q0YFW2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_44430</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000875">
        <property type="protein sequence ID" value="KAJ8017896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YFW2"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8017896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="283" mass="31941" checksum="E112045D15FA7737" modified="2023-09-13" version="1">MASRKRSYRYRVSCKECGKEMDSDWKNSHSRSKHGGRKVEYTAALPSSQSTLNFAPQKAASMDMTEGTSVVTGDNSSLQQDGTSGEILDQPGPSTSAEPVKVTTGIDLSSNPFVNVEGCETVHEVQSMEEMTQSHVVPETTQGEICEPQRPILSNYPGRKYGNETFERSFNPNLYKKYLWISYDVASDSCHCFPCKRFLGNLFEFSNWRKPEKLIKHEQSDKHCNQALSTWIEATAMEKQKSSVLKKLQATHAEEVQVNRNYLKAIIETVCFLCSTKHTTAWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YFX7</accession>
    <name>A0A9Q0YFX7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Sodium-coupled monocarboxylate transporter 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_40305</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="6132759at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="entry name" value="Sodium/glucose cotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051163">
        <property type="entry name" value="Sodium:Solute_Symporter_SSF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00813">
        <property type="entry name" value="sss"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42985:SF40">
        <property type="entry name" value="LD47995P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42985">
        <property type="entry name" value="SODIUM-COUPLED MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="10" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="87"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="130"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="162"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="192"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="241"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="280"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="326"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="381"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="410"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="442"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="562"/>
            <end position="584"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8020655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="653" mass="71486" checksum="58CF840A84CD8E92" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YGN8</accession>
    <name>A0A9Q0YGN8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_41623</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8019857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YGN8"/>
    <dbReference type="OrthoDB" id="441971at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon gypsy-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF15">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="2">
        <location>
            <begin position="148"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="346"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="360"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8019857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="371" mass="42235" checksum="459D8A32F9B9FB2A" modified="2023-09-13" version="1">MASDLEKEKSARKLRHCRVLLLTGTYPKGLNKNEKRMLRCLKEKFMVDSSGKLVTIEGNREVVIEQKEQEEIVKKVHEGQGGAHLGRDKTVQELAAKFYWKGISNDVIQCINKCSKCVQHKKPIRARAKSSVVEKEVLDQAEKPTAPLHSIPVSGTWELVGIDIVGPLPTTENGNKYLITLCDLFSRWPEAAAVPDKSADSVAKFLMEVMMRMGFVKVITNQDGEFVNLVNAKLSKKCGVQLSIVKECHFQANRMVKRFSDTLETTLSKVVNKDRNDWDDHVNSILFAYRTSVHTTTKLSPFVVMFGREAVLPIDLMHSSSQPSAQPTEPESYEKFVETMKKERKRIHERLMENVEQAQTRRKKHKDKNLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YHW9</accession>
    <name>A0A9Q0YHW9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_40186</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YHW9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8020562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="161" mass="17767" checksum="DB023044BF0FFCFE" modified="2023-09-13" version="1">MGAPAVCERSCGYGCTQQHVLDRTQYSRRELRSRTSFLGHAELETGVAARKFRRCNEKSRAAARACLGKQPALQARHAELETGVAARKFRRCNEKSRAAARACLGKQPALQTPHAELETGVAARKFRRCNEKSLADARACLGKQPALQHVRLLGWLEVSPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YHY3</accession>
    <name>A0A9Q0YHY3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Polycomb group protein Pc</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_40206</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YHY3"/>
    <dbReference type="OrthoDB" id="1918685at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035102">
        <property type="term" value="C:PRC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023779">
        <property type="entry name" value="Chromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052458">
        <property type="entry name" value="PcG_PRC1-like_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46389">
        <property type="entry name" value="POLYCOMB GROUP PROTEIN PC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46389:SF3">
        <property type="entry name" value="POLYCOMB GROUP PROTEIN PC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00598">
        <property type="entry name" value="CHROMO_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chromo" evidence="4">
        <location>
            <begin position="267"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="125"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="134"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="148"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="184"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="214"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="236"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8020577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="323" mass="35672" checksum="4A11A257DAA5B0A5" modified="2023-09-13" version="1">MPHHKGKINTNFVQNKKQRLKYKNRRIKTLLSKGMELCSMTNCSVFIYTKDELNHIRVAGSQDMLAAYHGAGIKSTMFSETESTSSEIVQGPTARPSAPTCSSNIMTCDAATACTSSSTASQQDKPAVALVCRRSHESEPHRSHSDNSPMPKTSPSATATSDSSVCNVPPRPTSSTACVEPTQEEVQLQNASTLSASENNLPHTGPDTGRHSPLNHTYTQPTTQPTEVNSPRPADDSDEEDADEEAPLSLVKAVLKLQQDDPLAQVYQAEKITDSRMFLGSKQYFVKWKGWSDKHNTWEPEEHIIDKKLIALFLSQQNKEKEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YI87</accession>
    <name>A0A9Q0YI87_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Low-density lipoprotein receptor-related protein 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_42395</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000066">
        <property type="protein sequence ID" value="KAJ8019186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YI87"/>
    <dbReference type="OrthoDB" id="10046193at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000241">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000013">
        <property type="entry name" value="Membrane frizzled-related protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050778">
        <property type="entry name" value="Cueball_EGF_LRP_Nidogen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003410">
        <property type="entry name" value="HYR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000033">
        <property type="entry name" value="LDLR_classB_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46513:SF44">
        <property type="entry name" value="LDL RECEPTOR RELATED PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46513">
        <property type="entry name" value="VITELLOGENIN RECEPTOR-LIKE PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00058">
        <property type="entry name" value="Ldl_recept_b"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00135">
        <property type="entry name" value="LY"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63825">
        <property type="entry name" value="YWTD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50825">
        <property type="entry name" value="HYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51120">
        <property type="entry name" value="LDLRB"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040453403" evidence="8">
        <location>
            <begin position="22"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="9">
        <location>
            <begin position="25"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="7">
        <location>
            <begin position="191"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="7">
        <location>
            <begin position="234"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="7">
        <location>
            <begin position="277"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="7">
        <location>
            <begin position="321"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="HYR" evidence="10">
        <location>
            <begin position="438"/>
            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8019186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="521" mass="57653" checksum="9415463C8751BFBC" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YM69</accession>
    <name>A0A9Q0YM69_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Integrin alpha-9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HOLleu_35059</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Nanhai2018</strain>
            <tissue evidence="18">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Membrane</location>
            <topology evidence="1 13">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the integrin alpha chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YM69"/>
    <dbReference type="OrthoDB" id="5317514at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008305">
        <property type="term" value="C:integrin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033627">
        <property type="term" value="P:cell adhesion mediated by integrin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.930">
        <property type="entry name" value="Bicelle-embedded integrin alpha(iib) transmembrane segment"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.130">
        <property type="entry name" value="Integrin alpha, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1460">
        <property type="entry name" value="Integrin domains. Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1510">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1530">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013517">
        <property type="entry name" value="FG-GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013519">
        <property type="entry name" value="Int_alpha_beta-p"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000413">
        <property type="entry name" value="Integrin_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018184">
        <property type="entry name" value="Integrin_alpha_C_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013649">
        <property type="entry name" value="Integrin_alpha_Ig-like_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048285">
        <property type="entry name" value="Integrin_alpha_Ig-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048286">
        <property type="entry name" value="Integrin_alpha_Ig-like_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028994">
        <property type="entry name" value="Integrin_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032695">
        <property type="entry name" value="Integrin_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23220">
        <property type="entry name" value="INTEGRIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23220:SF134">
        <property type="entry name" value="INTEGRIN ALPHA-2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01839">
        <property type="entry name" value="FG-GAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08441">
        <property type="entry name" value="Integrin_A_Ig_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20805">
        <property type="entry name" value="Integrin_A_Ig_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20806">
        <property type="entry name" value="Integrin_A_Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01185">
        <property type="entry name" value="INTEGRINA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00191">
        <property type="entry name" value="Int_alpha"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69318">
        <property type="entry name" value="Integrin alpha N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69179">
        <property type="entry name" value="Integrin domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51470">
        <property type="entry name" value="FG_GAP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00242">
        <property type="entry name" value="INTEGRIN_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 13 18" id="KW-0401">Integrin</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 13" id="KW-0732">Signal</keyword>
    <keyword evidence="3 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040535967" evidence="13">
        <location>
            <begin position="20"/>
            <end position="1111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1051"/>
            <end position="1074"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="12">
        <location>
            <begin position="23"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="12">
        <location>
            <begin position="309"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="12">
        <location>
            <begin position="370"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="12">
        <location>
            <begin position="433"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin alpha first immunoglubulin-like" evidence="15">
        <location>
            <begin position="480"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin alpha second immunoglobulin-like" evidence="16">
        <location>
            <begin position="650"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin alpha third immunoglobulin-like" evidence="17">
        <location>
            <begin position="856"/>
            <end position="1036"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1092"/>
            <end position="1111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF08441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF20805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF20806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ8024984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1111" mass="122884" checksum="9E7F95C161CA2111" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YN33</accession>
    <name>A0A9Q0YN33_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA-directed DNA polymerase</fullName>
            <ecNumber evidence="2">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_35551</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="2"/>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YN33"/>
    <dbReference type="OrthoDB" id="5871067at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.960.10">
        <property type="entry name" value="VSR Endonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004868">
        <property type="entry name" value="DNA-dir_DNA_pol_B_mt/vir"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33568">
        <property type="entry name" value="DNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33568:SF3">
        <property type="entry name" value="DNA-DIRECTED DNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03175">
        <property type="entry name" value="DNA_pol_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0235">DNA replication</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA-directed DNA polymerase family B mitochondria/virus" evidence="10">
        <location>
            <begin position="635"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="37"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="540"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="72"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="114"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8023217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1045" mass="119760" checksum="269DAAD2716EC949" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YN99</accession>
    <name>A0A9Q0YN99_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_36229</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YN99"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8023714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="148" mass="18125" checksum="AF7B78A1977C1AE1" modified="2023-09-13" version="1">MRFLACYRSVLSWAKPTTFPFWWVMRFFTCLSNQFCHRSLFYWVKPTSFLIRCVMRFLACVFNLFCNSGRFFCWVKPTTIPFNWRMRFLACFSNLFHSRRSFSSWIKPASISFSWVMRLLAHLFNLISSNRSLLYRVRQTPFFFRLMM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YQ19</accession>
    <name>A0A9Q0YQ19_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Mu-type opioid receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_34544</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YQ19"/>
    <dbReference type="OrthoDB" id="10044919at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00637">
        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24228">
        <property type="entry name" value="B2 BRADYKININ RECEPTOR/ANGIOTENSIN II RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24228:SF72">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="46"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="79"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="122"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="163"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="220"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="273"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="299"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="10">
        <location>
            <begin position="62"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8024599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="361" mass="40685" checksum="E442F9F6FEC62D11" modified="2023-09-13" version="1">MESTDFLQIGFTTSSLLETSVSTLNASLNGTTEGPPWVFDDINQRIVVAALLFVIAVFGLTGNILTIVAVILSKKLHTIANVFVVNLTIAGGLSCATLPFTIANMIANTDKGIIPDGLCTFVSLMSYFSFSGTIVSHALIAFDRYYRITKPCHKYHKMFTKCRITIFIICAWIYANISVLFAIITNVNIMYSQKYRVCLPDIVDPDVAKVSLFRATTIDLFILILVMICYTAMFIYIYRHNKKLLGMFNVSKTEGVNLRTTLNARQVKITMNMFLVVAVYVVCITPYVVCLIVDSLETIFPYVAVFYALSNAINPAIYAWKHPYFRAVFKPMIQLKCSKIPNPSPILKFFTGYESRRNRMW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YRG9</accession>
    <name>A0A9Q0YRG9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Elongator complex protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_35495</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="3">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the WD repeat ELP2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8025319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YRG9"/>
    <dbReference type="OrthoDB" id="27911at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033588">
        <property type="term" value="C:elongator holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000400">
        <property type="entry name" value="Elongator acetyltransferase complex subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037289">
        <property type="entry name" value="Elp2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44111">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44111:SF1">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="7 11" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="212"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="396"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="674"/>
            <end position="705"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8025319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="820" mass="91221" checksum="2BFBED252C618D65" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YRP8</accession>
    <name>A0A9Q0YRP8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">histone acetyltransferase</fullName>
            <ecNumber evidence="4">2.3.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HOLleu_35274</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Nanhai2018</strain>
            <tissue evidence="18">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-lysyl-[histone] + acetyl-CoA = N(6)-acetyl-L-lysyl-[histone] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21992"/>
            <dbReference type="Rhea" id="RHEA-COMP:9845"/>
            <dbReference type="Rhea" id="RHEA-COMP:11338"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.48"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:21993"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the acetyltransferase family. GCN5 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="4"/>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8025152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YRP8"/>
    <dbReference type="OrthoDB" id="1937912at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140672">
        <property type="term" value="C:ATAC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043992">
        <property type="term" value="F:histone H3K9 acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000004">
        <property type="entry name" value="Histone acetyltransferase KAT2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037800">
        <property type="entry name" value="GCN5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009464">
        <property type="entry name" value="PCAF_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45750">
        <property type="entry name" value="GH11602P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45750:SF3">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06466">
        <property type="entry name" value="PCAF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="12" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7 14" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="17">
        <location>
            <begin position="502"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="16">
        <location>
            <begin position="739"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="346"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="346"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="375"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ8025152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="768" mass="87688" checksum="E8A4E3F68154E092" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YSE9</accession>
    <name>A0A9Q0YSE9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_33298</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YSE9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8025677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="137" mass="15614" checksum="CD995D4015235FAA" modified="2023-09-13" version="1">MWGHQGSKTKNLVCTISQDALWREDECCSFWWRSKVIWGHQQSNTENLLLKQYLKMTYGEKMNPVIFGGGQRSFGVTESQILKTLLTQYLKIHHSEKLCHTVLGGGQRSSGVTKGQKLITLLLAQYLKVGSLDKFHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1B9E0</accession>
    <name>A0A9Q1B9E0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transposable element Tc3 transposase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_44330</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000802">
        <property type="protein sequence ID" value="KAJ8017945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1B9E0"/>
    <dbReference type="OrthoDB" id="6021633at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038717">
        <property type="entry name" value="Tc1-like_DDE_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13358">
        <property type="entry name" value="DDE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tc1-like transposase DDE" evidence="2">
        <location>
            <begin position="3"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8017945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="96" mass="11466" checksum="9A98FA8F7F9B1D3D" modified="2023-09-13" version="1">MQDNDPKHTSAFARDFFKTANIVWWKTPPESPDLNPIEKIWHELKHFLRSHHKPTTKDQLVDGILTFWKHDTPRRTLVKQKDPKPDRQKRNCSLAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BAA9</accession>
    <name>A0A9Q1BAA9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_44671</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010001102">
        <property type="protein sequence ID" value="KAJ8017719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BAA9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8017719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="64" mass="7234" checksum="7E1B7AFED2E6BB05" modified="2023-09-13" version="1">MLSCLLHLSTWQGITTPLGVLVEIKPVSGQYLKIYDMMCANICFSFRETWQDAQKPVLGSILIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BDA8</accession>
    <name>A0A9Q1BDA8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Serrate RNA effector molecule-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_40002</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ARS2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BDA8"/>
    <dbReference type="OrthoDB" id="342064at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016604">
        <property type="term" value="C:nuclear body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031053">
        <property type="term" value="P:primary miRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039727">
        <property type="entry name" value="SE/Ars2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007042">
        <property type="entry name" value="SERRATE/Ars2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13165">
        <property type="entry name" value="ARSENITE-RESISTANCE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13165:SF0">
        <property type="entry name" value="SERRATE RNA EFFECTOR MOLECULE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04959">
        <property type="entry name" value="ARS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SERRATE/Ars2 C-terminal" evidence="5">
        <location>
            <begin position="545"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="71"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="382"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="609"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="76"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="96"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="122"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="146"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="186"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="230"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="398"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="431"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="621"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="634"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="648"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="671"/>
            <end position="680"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8020417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="748" mass="84133" checksum="239293EC9B983863" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BF24</accession>
    <name>A0A9Q1BF24_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Enkurin domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_37124</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BF24"/>
    <dbReference type="OrthoDB" id="10264920at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005881">
        <property type="term" value="C:cytoplasmic microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027012">
        <property type="entry name" value="Enkurin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052102">
        <property type="entry name" value="Enkurin_domain-protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21490:SF2">
        <property type="entry name" value="ENKURIN DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21490">
        <property type="entry name" value="ENKURIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13864">
        <property type="entry name" value="Enkurin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51665">
        <property type="entry name" value="ENKURIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enkurin" evidence="8">
        <location>
            <begin position="223"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="57"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="283"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="25"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="65"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="151"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8022277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="318" mass="36511" checksum="9DEC8CE6CDE041C7" modified="2023-09-13" version="1">MISSPIPYDPGYGSVVGRRNSWAGQEERPKPRVKPEAEEIANKAKGTIANLLVTGQQVYRPPVKQKKEPKDFSQDNVRKMRQIQALNRRRRKEEEEEKNKPMKVLPQSEKYKEVSSKVSENLKQSPPPPRPASANFLRAHSRTGGFKPRGRSPSPASRPSSAAVEVDIEPPKPTNFIAHNAKLTKKYKPPRPPSALAIEGHEQKKQQQLQNYKKGEVPKYLKSRQKQWQQEEEERIRNTPDPDMPPGHRMMPTEERLKTLEILAEREKLLRDELHRLPLAPETLRAKSRKAEVEKQLAEVEEATKIFSRQKVFVKIDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BGC3</accession>
    <name>A0A9Q1BGC3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Pancreatic triacylglycerol lipase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_33081</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BGC3"/>
    <dbReference type="OrthoDB" id="199913at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016298">
        <property type="term" value="F:lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00707">
        <property type="entry name" value="Pancreat_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002334">
        <property type="entry name" value="Allerg_PlipaseA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013818">
        <property type="entry name" value="Lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033906">
        <property type="entry name" value="Lipase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000734">
        <property type="entry name" value="TAG_lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610">
        <property type="entry name" value="LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610:SF178">
        <property type="entry name" value="LIPASE MEMBER H-A-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00151">
        <property type="entry name" value="Lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00825">
        <property type="entry name" value="DOLALLERGEN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00821">
        <property type="entry name" value="TAGLIPASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040346142" evidence="5">
        <location>
            <begin position="21"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="Lipase" evidence="6">
        <location>
            <begin position="22"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8025505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="346" mass="38060" checksum="5566A6F2132ACDA9" modified="2023-09-13" version="1">MKHFFVVLLGITVACKSVWGAQVCFDELGCFTDDTFCSDNNFPPWDPEAIKTQFFLYTRNDPSQYEVLDRKDAASVTSSKYDGSKGTKFIIHGYTDTIFGERFLDIKDRLLAKEDLNVIMVDWAKGADSLYDKARQNIRVVGMEIALLTALLNSQFGADFSKMHFIGHSLGAHTAGYAGAAQPGYGRITGMDNFTSAGLDPAGPGYRNVLDSCKLDPSDALFVDCIHTDGDYIFQGGAGLLEPLGHVDIYPNGGKDMPGCFLLDPVCDHSRVLYYFAESISSESCVFTAYPCDSWDNFRRGRCSLSCDAGECTQMGYNADLFSATGSFYLQTESRSPYCDSGSYVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BH13</accession>
    <name>A0A9Q1BH13_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_34017</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8026225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BH13"/>
    <dbReference type="OrthoDB" id="9950135at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09274">
        <property type="entry name" value="RNase_HI_RT_Ty3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.370:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 17.6-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000003">
        <property type="entry name" value="Transposon Ty3-G Gag-Pol polyprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000026">
        <property type="entry name" value="Transposon Ty3-G Gag-Pol polyprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041373">
        <property type="entry name" value="RT_RNaseH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17917">
        <property type="entry name" value="RT_RNaseH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50804">
        <property type="entry name" value="SCAN_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SCAN box" evidence="10">
        <location>
            <begin position="77"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="9">
        <location>
            <begin position="200"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="163"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="172"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8026225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="791" mass="89272" checksum="D2B6903012AE2B62" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BHV3</accession>
    <name>A0A9Q1BHV3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">F-box only protein 30</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_33608</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BHV3"/>
    <dbReference type="OrthoDB" id="5918172at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.150">
        <property type="entry name" value="TRAF-like zinc-finger, N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031890">
        <property type="entry name" value="Fbxo30/Fbxo40"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001293">
        <property type="entry name" value="Znf_TRAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043013">
        <property type="entry name" value="Znf_TRAF_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15933:SF20">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15933">
        <property type="entry name" value="PROTEIN CBG16327"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15966">
        <property type="entry name" value="F-box_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15965">
        <property type="entry name" value="zf-TRAF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50145">
        <property type="entry name" value="ZF_TRAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="4 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TRAF-type" evidence="7">
        <location>
            <begin position="37"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="F-box" evidence="8">
        <location>
            <begin position="257"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="TRAF-type" evidence="5">
        <location>
            <begin position="37"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="142"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="142"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8025909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="324" mass="37034" checksum="70263523D9DEC81E" modified="2023-09-13" version="1">MAGVNREDPEPVHGHCTNCVSVNCKVLPITDHSCPMVTCPADCNAKFHRCKIDDHRNLCPNEVVPCLNHWNGCKKRMPRFSAKKHLKVCPSSHRGYSEDFKFVNFYVALKHYQEEEANKDLTDSESIHSKVSDTYESFHINDSSQSEMSNANESLHTNDSSQSEMTDANEPVHNDDSCQQQIADESKLVHTIANNISNGFCLLRKSLWVVPWQVQKSPEHEQFIQIHSSKCKCNDPSDQEMAPIMLPSDETHSEMSGFRMDELPYMTLRKIAGFLDAESLSKLSLVSYQMRMVCASWLHDHGSVTVIWEKINGKWVDSKRIWKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BHV6</accession>
    <name>A0A9Q1BHV6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Transmembrane emp24 domain-containing protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_33630</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the EMP24/GP25L family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BHV6"/>
    <dbReference type="OrthoDB" id="3427at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015720">
        <property type="entry name" value="Emp24-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22811">
        <property type="entry name" value="TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01105">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01190">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50866">
        <property type="entry name" value="GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040135624" evidence="8">
        <location>
            <begin position="21"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="9">
        <location>
            <begin position="30"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8025924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="218" mass="25554" checksum="17DD6704CF7ED76B" modified="2023-09-13" version="1">MELLKSTLILLLALISASHCLYFHIGETEKKCFIEEIPDETMVIGNYKTQLYDKKTNTFMPSSPGIGMHVEIKGPDDDVILSKMYSSEGRFTFTSHTPGEHLICIYSNSTSWAMFGSNKMRVHLDIEVGEHANDYREIQEKDKLTELQLRVRQLLDQVEQIQKEQNYQRYREERFRHTSESTNQRVLWWSIAQTAILLLTGFWQMRHLKGFFEAKKLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BJG3</accession>
    <name>A0A9Q1BJG3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">ER membrane protein complex subunit 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_29755</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EMC7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8027722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BJG3"/>
    <dbReference type="OrthoDB" id="27095at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072546">
        <property type="term" value="C:EMC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019008">
        <property type="entry name" value="Beta_sandwich_EMC7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039163">
        <property type="entry name" value="EMC7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13605">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13605:SF4">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09430">
        <property type="entry name" value="EMC7_beta-sandw"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040201421" evidence="9">
        <location>
            <begin position="25"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="158"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="ER membrane protein complex subunit 7 beta-sandwich" evidence="10">
        <location>
            <begin position="53"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="210"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="217"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF09430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8027722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="229" mass="25839" checksum="09544F3C57ABCC22" modified="2023-09-13" version="1">MKTVYVRTVLCILTRLFLLLLAYGEDAVDSAKRNPNELFTIEGKASVPGMKASEWIPATKILIDGGTYQGFLKSDGSFQLFVPTGSYVIDLTHPALDFEPQRVDVNSKGNKRARKLNNIQPNAVSHLTYPLRFKPKGQMNYFQKREEFRIIDILKSPMVIMMVLPIVFLVLIPKLINTDDPEIKKEMQSLNLMNPKQDMPDLSEMVTNLFGGGAPPQKKKEKAVKGKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BK00</accession>
    <name>A0A9Q1BK00_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_30184</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BK00"/>
    <dbReference type="OrthoDB" id="10068277at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8028057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="293" mass="33319" checksum="B0BC61D1C0D7EC74" modified="2023-09-13" version="1">MAPSFRRLRNHAGEIEFLSKCSPRQRKAFLKHAYPELVASLCECASNVLKGNVPLSRAQKSKLSQYKKHLRDLSDNRLSNKKRKDILVQRGGFLSLLLKPIIQSLGGMLLGAKKTLREPIELRVAHDSTPSTNNQTTDTISGKISEPDDLIHNFPKSIKGKAKVLLERIDRHCSTQNDPTVDWNSKGELLYKGEAVPGSNITDLIIDVLQSRKDFNPNGWQHFISGLKGINFPEAFVGNLKRRQFMHQLKEKDKSELFVKELPTLPRSKRRRKVISSPASLQKRLKSIRWDIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BKZ6</accession>
    <name>A0A9Q1BKZ6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_30805</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BKZ6"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF13">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8028538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="262" mass="31256" checksum="60548D948DC608FF" modified="2023-09-13" version="1">MPFNLDKFVTSPSVEELDSLKKSEIVKVAKHYGIEFQPLMRKDEIKRYVLEYLVDEGVLPSTVLETAITVPTDNTFELKRLQLEMNKEIRLKEMEREMQKEKEEREMQKEKEERGMQMQREKEEREERERREEKAREHEFRLKQLELGVIKGSDPKIGLDTGGFDVSKHVKFVPKFQEDNVEKFFNHFEKLGEQLEWPRDKWSILIQSNFTGKAQEVYSALSIEDSMDYDKVKKAILQAYELVPEAYRQNLESIVRRIHKHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BM18</accession>
    <name>A0A9Q1BM18_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 7">5-formyltetrahydrofolate cyclo-ligase</fullName>
            <ecNumber evidence="5 7">6.3.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_28565</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4 7">
            <text>(6S)-5-formyl-5,6,7,8-tetrahydrofolate + ATP = (6R)-5,10-methenyltetrahydrofolate + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10488"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57455"/>
            <dbReference type="ChEBI" id="CHEBI:57457"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the 5-formyltetrahydrofolate cyclo-ligase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.3.2" evidence="5 7"/>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BM18"/>
    <dbReference type="OrthoDB" id="2015992at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030272">
        <property type="term" value="F:5-formyltetrahydrofolate cyclo-ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009396">
        <property type="term" value="P:folic acid-containing compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10420:FF:000007">
        <property type="entry name" value="5-formyltetrahydrofolate cyclo-ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10420">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002698">
        <property type="entry name" value="FTHF_cligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024185">
        <property type="entry name" value="FTHF_cligase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02727">
        <property type="entry name" value="MTHFS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23407:SF1">
        <property type="entry name" value="5-FORMYLTETRAHYDROFOLATE CYCLO-LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23407">
        <property type="entry name" value="ATPASE INHIBITOR/5-FORMYLTETRAHYDROFOLATE CYCLO-LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01812">
        <property type="entry name" value="5-FTHF_cyc-lig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006806">
        <property type="entry name" value="FTHF_cligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="10"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="144"/>
            <end position="152"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00036539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR006806-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8029223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="202" mass="23038" checksum="67D3ADB990A81B24" modified="2023-09-13" version="1">MSVSVVRETKRILRKELKKWIAGLTEEERQLQSENVVKQLLGHPVYQKSKRISVYLSMPQEEVNTEGILRHMFTNGKICFVPQYIGPKMEMLRLHSMEDYESLPVTKWNIKQPAEGDAREEALSSGGLDLIIMPGLGFTLEGDRLGRGKGYYDKYLQRCAEHSTGMPATIAIAFKEQIAEKLPVTENDRRVDEVLYGYKDGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BM68</accession>
    <name>A0A9Q1BM68_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_28389</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BM68"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="29"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8029076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="151" mass="16801" checksum="E4E62DDFEAD96292" modified="2023-09-13" version="1">MNSLETLLQSLNELIEMEVDSPEETQLVGSTGSISSSSKSGGKDSSRASARSSKKLGYATKAAALRTKLKYVDIEEAKTAELKRIKTLKELDVAEAKFNALSKLDQDIEENTHNWCQMAEMNMCMNMLKTCSWKGSFRICSCLSYYKCSQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BNT0</accession>
    <name>A0A9Q1BNT0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Death domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_29346</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BNT0"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01670">
        <property type="entry name" value="Death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000488">
        <property type="entry name" value="Death_dom"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50017">
        <property type="entry name" value="DEATH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Death" evidence="2">
        <location>
            <begin position="616"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="137"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="251"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="332"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="430"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="499"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="137"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="148"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="333"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8029839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="694" mass="78089" checksum="80390F70D3DBA6EF" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BP06</accession>
    <name>A0A9Q1BP06_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Peripheral plasma membrane protein CASK</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">HOLleu_26491</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="22">Nanhai2018</strain>
            <tissue evidence="22">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MAGUK family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="14">In the N-terminal section; belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BP06"/>
    <dbReference type="OrthoDB" id="65789at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030054">
        <property type="term" value="C:cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10831">
        <property type="entry name" value="PDZ_CASK-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12035">
        <property type="entry name" value="SH3_MPP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14094">
        <property type="entry name" value="STKc_CASK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.63.10:FF:000002">
        <property type="entry name" value="Guanylate kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000146">
        <property type="entry name" value="MAGUK p55 subfamily member 6 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000051">
        <property type="entry name" value="Peripheral plasma membrane protein CASK isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000062">
        <property type="entry name" value="peripheral plasma membrane protein CASK isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000016">
        <property type="entry name" value="peripheral plasma membrane protein CASK isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.650">
        <property type="entry name" value="L27 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008144">
        <property type="entry name" value="Guanylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020590">
        <property type="entry name" value="Guanylate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014775">
        <property type="entry name" value="L27_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004172">
        <property type="entry name" value="L27_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036892">
        <property type="entry name" value="L27_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050716">
        <property type="entry name" value="MAGUK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23122">
        <property type="entry name" value="MEMBRANE-ASSOCIATED GUANYLATE KINASE MAGUK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02828">
        <property type="entry name" value="L27"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00569">
        <property type="entry name" value="L27"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101288">
        <property type="entry name" value="L27 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00856">
        <property type="entry name" value="GUANYLATE_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50052">
        <property type="entry name" value="GUANYLATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51022">
        <property type="entry name" value="L27"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3 16" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="18">
        <location>
            <begin position="12"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="L27" evidence="21">
        <location>
            <begin position="339"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="L27" evidence="21">
        <location>
            <begin position="417"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="20">
        <location>
            <begin position="502"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="17">
        <location>
            <begin position="589"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate kinase-like" evidence="19">
        <location>
            <begin position="717"/>
            <end position="890"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00060907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00071925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KAJ8030161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="905" mass="102929" checksum="4488E4828CCC8C5E" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BQS5</accession>
    <name>A0A9Q1BQS5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_27451</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BQS5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8030906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="229" mass="26085" checksum="3D24E5B28FDEA3CB" modified="2023-09-13" version="1">MATETSRNQTNISNQTGPNLSSNEGVRRGNPPKCSSCGKSKILIEDDKDSTKSVFVCLNQECDQYYYPPTCSCGKTKSLIEDDKDSTKSVFVCLNQECDEYYYTPTCSCGKSKFLIEDDKDSTKSVFVCLNRKCDEYYYPPTCSCGKRKSLIEDDKDSKKSVFVCLNRKCDGYYYRPTCSSCGAKGSLISGYDSSTESNFVCLTIWCDKYHYKRRRDDSDSWWSWCSIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BRP4</accession>
    <name>A0A9Q1BRP4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_24659</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BRP4"/>
    <dbReference type="OrthoDB" id="6157407at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040408205" description="Ig-like domain-containing protein" evidence="3">
        <location>
            <begin position="20"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="341"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="42"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="478"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8031463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="516" mass="56755" checksum="E7B7D48891C6B2AD" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BRV1</accession>
    <name>A0A9Q1BRV1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SCAN box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_24737</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BRV1"/>
    <dbReference type="OrthoDB" id="8963689at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF13">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCAN box" evidence="2">
        <location>
            <begin position="234"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="327"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="327"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="343"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="367"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8031524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="377" mass="44264" checksum="3FF0416FDEEC4AED" modified="2023-09-13" version="1">MPFNLDKFVASPSVEELDSLKKSEIVKVAKHYGIEFQPLMRKDEIKRYVLEYLVDESVLPSTVLETAITVPTDNTFELKRLEIEMNKEIRLKEMERETQKEKEEREMQMQREKEEREERERREEKAREHEFRLKQQELGVIKGSDPKIGLDTEGFDVSKHVKFVPKFQEDNVEKFNHFEKLGEQLKWPRDKWSILIQSNFTGKAQEVYSALSIVDSMDYDKVEKVILQAYELVPEAYRQKFRKYRKADTQTYVEFAYQKERHFDRWCASKKVSTFDALRQLMLVEEFKRCVNDDIKTHLEENKADKIKEVANLADQYALTHKFGKVGQTQNKDQFSRPNKKYGNSSGTTGNSGSQSGSISPTNSGKKQPDSSRQKND</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BSE7</accession>
    <name>A0A9Q1BSE7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Fucosyltransferase</fullName>
            <ecNumber evidence="11">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_25099</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Endomembrane system</location>
            <topology evidence="10">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Golgi apparatus</location>
            <location evidence="11">Golgi stack membrane</location>
            <topology evidence="11">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the glycosyltransferase 10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="11"/>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BSE7"/>
    <dbReference type="OrthoDB" id="427096at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046920">
        <property type="term" value="F:alpha-(1-&gt;3)-fucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11660:FF:000002">
        <property type="entry name" value="Alpha-(1,3)-fucosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11660">
        <property type="entry name" value="Glycosyl transferase family 10, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055270">
        <property type="entry name" value="Glyco_tran_10_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031481">
        <property type="entry name" value="Glyco_tran_10_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001503">
        <property type="entry name" value="Glyco_trans_10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038577">
        <property type="entry name" value="GT10-like_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11929">
        <property type="entry name" value="ALPHA- 1,3 -FUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11929:SF145">
        <property type="entry name" value="ALPHA-(1,3)-FUCOSYLTRANSFERASE FUT-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17039">
        <property type="entry name" value="Glyco_tran_10_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00852">
        <property type="entry name" value="Glyco_transf_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="15"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="domain" description="Fucosyltransferase N-terminal" evidence="13">
        <location>
            <begin position="122"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="Fucosyltransferase C-terminal" evidence="12">
        <location>
            <begin position="223"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00060399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF17039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ8031790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="431" mass="49691" checksum="5A437A390A20F5DE" modified="2023-09-13" version="1">MEESSTSFFSSMRTLLMLAIVISSVTVVILIVVTEFGYPRPKIRLINEEQTFPFYDNTSLRRREENVLFNLSSNIKKDHGHSNCSILLVVKGYFLYFQKAISKSRTIWCPSVNCTVSLLYSNNISDVLSSNITFFSHLTRLPSAKKLRYLLEHRRPEQRWALMSLETPLRVARNTSLPASISKNFFHWLATYDPASEIPITYGQVVSFTNGDFKPQRVNYFELKSKMVAFVSSNCIHSKWRRLDFVKALQRYIPVDIYGKCGNLTCDVEADCTDILRQYKFILALENSPCPGYITEKFWNAVGKFESVAVVWGANAYDYEQYAPPQSFILVESFPSIKHLASHLWNVSSNKTLYNTLHDWRMQYSVRSQDKLATLPNNEALCRAADLYINETTDFLSKMGGARYKHTNGKDWIQSCRIPVHTKMKEFPIPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BSJ0</accession>
    <name>A0A9Q1BSJ0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Rho GTPase-activating protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_25208</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BSJ0"/>
    <dbReference type="OrthoDB" id="19923at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001136">
        <property type="term" value="P:negative regulation of endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45808">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 68F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45808:SF2">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 68F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13716">
        <property type="entry name" value="CRAL_TRIO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="2">
        <location>
            <begin position="71"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="3">
        <location>
            <begin position="255"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="460"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="468"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="480"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="519"/>
            <end position="554"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8031865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="564" mass="64102" checksum="1AE453C868385B17" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BUG4</accession>
    <name>A0A9Q1BUG4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_22848</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8032796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BUG4"/>
    <dbReference type="OrthoDB" id="6077919at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF1">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="307"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="335"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8032796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="386" mass="44224" checksum="0C5096AD18B1BFDD" modified="2023-09-13" version="1">MTRGKITKDEMETAESGNEQTPHSIPEGLPKEMWVQYLIDMQKLQHEKDMKLQTEKLQHDRELEKIKAEKEIELRKLEFSERSSSGSLHGNSQENLNMTYGYDVRRVPRLRDEDDIDVYLRAFELLAEGNGWDKSQWAKLLIPSLSGKAREVYANLSVADSQNYDVLKTAILAKYEINAESYRIKFRNTCRKPDETIAEWINDLSYFLDGWLKFSDISQDSVQAIKELLIVEQAIGRFPDDLAIYLKDKQVKTAKDLAQAADQYVINRGGKEYWKNVGRKVVPTWQTANKGNGKSLNPNTQLYKNVKCFNCGKIGHTSKICTQGKSDQNKTVQTRKCFKCGQEGHLRSECPQNAVQSHGNGAQPKPLYKCQVDQSYDIARDRINST</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C0S3</accession>
    <name>A0A9Q1C0S3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_17740</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C0S3"/>
    <dbReference type="OrthoDB" id="8053926at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33332">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8037023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="194" mass="22601" checksum="ED2249886C807690" modified="2023-09-13" version="1">MACCDQVHNLGVIFNKHMKMDKHISKVCKAGFYYIFNLKKIRKHMSSETMSTMVHSFIKSHLDYCNSLLNSKPVYLIQRLQNSAARLVVDCYNFNTPSLSIILSLHSWLPVEFRIKFKVLLIVCRCIHGMAPTHLSNNLTFQVKTRYTSRFYTYPHCSQNYVENLRRSCLSVSWALPLESSPHFYQKLSTSHTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C1Q2</accession>
    <name>A0A9Q1C1Q2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_20592</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C1Q2"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040165130" evidence="1">
        <location>
            <begin position="30"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8036575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="157" mass="17973" checksum="4E186501147130A7" modified="2023-09-13" version="1">MESHSGDWTLQFACKIIFIIVLGTQLIQAESFNCRKFCNNCLKLPSHHKCYRICIPPGLCGQQAPGKRAYERPVREEPHPFDTSINRKTSNSLNAFLPYHPVPVKSTQCVLDKLFLSYPPLVQAKIIEVFREAEKTLQDMRDILDLEINFSNDVPPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C201</accession>
    <name>A0A9Q1C201_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Monocarboxylate transporter 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_18568</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C201"/>
    <dbReference type="OrthoDB" id="6499973at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008028">
        <property type="term" value="F:monocarboxylic acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF172">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="105"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="140"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="169"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="245"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="285"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="316"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="374"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="405"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8037683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="505" mass="55221" checksum="D736251F38533290" modified="2023-09-13" version="1">MRRLCFNLHFSNRKGLVVLAASTWLNILIYAPLTVFAILFVSLEDEFGASTTAIGEIGSAAWAVFNLSSPLPTFLIQKWGSRIVAATGVLMCGSGLLLSTISKQIWMLFVFYGVVQGLGLCFVQMASLQLLTCYFSGSALVRALSVAALGETIGMFAVSPIFTIGIEKLGWRLICQITALVIVTLCLPTCYVLGSKTHARSSYTSPPHDNTVTKDCKKEEAEDYSKPRGVLKIVKKKTLTEIWRIPGIWIFAIATVLYVLCMGLIMISLVDILVSNGHSMKESSLFVTIFAISQTIGKIVNIIIGNRLPVTNMKILVLMCITGGLSSLGMVYWEDRGTYILVFCIVNGLIRSNISSRNLPAAIEVFGESTSPILINVVCISIGIAFLCASLVGGLSYDLTGSYRAAHIAVFSSWFLAAVLLLPLAFLWPSSFFQKLYCCSEKYSKIQPRTADCEHSAVEVRQNGKTDVMAPSSVEYSNVNGFQGDLYRETVNDMLMSSAFKESRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C4H2</accession>
    <name>A0A9Q1C4H2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Microsomal glutathione S-transferase 1</fullName>
            <ecNumber evidence="5">2.5.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HOLleu_15652</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Nanhai2018</strain>
            <tissue evidence="17">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:16438"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="14">Homotrimer; The trimer binds only one molecule of glutathione.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the MAPEG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="5"/>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C4H2"/>
    <dbReference type="OrthoDB" id="193139at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.550">
        <property type="entry name" value="Membrane associated eicosanoid/glutathione metabolism-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023352">
        <property type="entry name" value="MAPEG-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001129">
        <property type="entry name" value="Membr-assoc_MAPEG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040162">
        <property type="entry name" value="MGST1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10689">
        <property type="entry name" value="MICROSOMAL GLUTATHIONE S-TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10689:SF6">
        <property type="entry name" value="MICROSOMAL GLUTATHIONE S-TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01124">
        <property type="entry name" value="MAPEG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161084">
        <property type="entry name" value="MAPEG domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00038540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00039397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ8038275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="132" mass="15133" checksum="797A5FBE9ADDE44A" modified="2023-09-13" version="1">MFVTVSIFLVFNRHVQVFLTKEDYVGFSGDLELDDIRRRLSVKDPEIERIRSCHRNDMENIPAFLAVGLLYVITNPREDLVSLHFRIFTLSRIVHTIAYLLPLPQPSRFLGFLIGTLSIVSMAVQTLISGKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C534</accession>
    <name>A0A9Q1C534_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_15788</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C534"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8038378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="50" mass="5920" checksum="74A6EA6A386FBBD9" modified="2023-09-13" version="1">MEAYKQSSGILHIIKSLQPLSRRKVLHHTQTSLSHFKQKKRTHQLLQTQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C559</accession>
    <name>A0A9Q1C559_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Histone H3, embryonic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_17178</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the histone H3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C559"/>
    <dbReference type="OrthoDB" id="842664at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22911">
        <property type="entry name" value="HFD_H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000085">
        <property type="entry name" value="Histone H3.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000164">
        <property type="entry name" value="Histone_H3/CENP-A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45810:SF17">
        <property type="entry name" value="HISTONE H3-LIKE CENTROMERIC PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45810">
        <property type="entry name" value="HISTONE H3.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00622">
        <property type="entry name" value="HISTONEH3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00428">
        <property type="entry name" value="H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="10">
        <location>
            <begin position="2"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8039453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="91" mass="10427" checksum="FFE668206908FC18" modified="2023-09-13" version="1">MEIRKYQKSTDLLMRKLPFARVVREVAQTFSIKDLRWQAVAIMALQEAAEAFLVHLFEDANLCALHAKRVTIYPKDIQLSRRIRGAEGGLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C5C4</accession>
    <name>A0A9Q1C5C4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_15900</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C5C4"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8038465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="161" mass="18409" checksum="731BD4C4613CAC18" modified="2023-09-13" version="1">MGGKSHEEQVSVKNGGQLVTIDDTDDGFTVILDYSDGIAVVKNTSSLECYFLPVDKFPDYNTDDMGSLTPVIVQNKTQANDENMRDVKPMASFVKSDIIPSEFMKTISDPSIAHQCGDSTSYWLTEAREKSVQKRQLYICICVDIYYWYGWIIYDYYYCYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C7X4</accession>
    <name>A0A9Q1C7X4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_13934</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8039815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C7X4"/>
    <dbReference type="OrthoDB" id="7920740at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="212"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="52"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="113"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="179"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="61"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="113"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="194"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="214"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="225"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8039815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="255" mass="29364" checksum="4FB9BE13E9CC0B78" modified="2023-09-13" version="1">MESPAAGEMFAEYGGSKSTRVGQTKWRVCEDQIWLTSRHHYAEVDGCDFWGKSSLPREEASTGSRNGVPGKQTNKGNIRWSQANERQHLSKLSPTNPFRTDVGLEWYESREQLESRKSESRRSRNFTEGETRRSDSEMNVANEELMRTASEEMKEFKLEIAELLQEVKNMQSELQILKNERTEYGSQGRQGKASPRRKFRRNRAYKKNGTLKCFKCRKPGHMKRNCPDRGGAAEKQVEQSSENDQLSGMGVTRRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C880</accession>
    <name>A0A9Q1C880_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_13974</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8039844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C880"/>
    <dbReference type="OrthoDB" id="6150275at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8039844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="124" mass="14739" checksum="CCE196AC66186AC3" modified="2023-09-13" version="1">MAVELEIIAEGSVENVMSGKKYNHAVHFHKLMYEALLRLAWRGFYSWLEEKDAANLPQLHETIKLIEELNTDVSQETFCNILQHPSCQCILLQFELYLDHKRSSNGSLSNFWTLTWTWWIFFWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C9B4</accession>
    <name>A0A9Q1C9B4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Tetratricopeptide repeat protein 39B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_14698</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C9B4"/>
    <dbReference type="OrthoDB" id="43460at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019412">
        <property type="entry name" value="IML2/TPR_39"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31859">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 39 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31859:SF9">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 39B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10300">
        <property type="entry name" value="Iml2-TPR_39"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="544"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="548"/>
            <end position="558"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8040416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="564" mass="64387" checksum="B8D28722436301EF" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C9C4</accession>
    <name>A0A9Q1C9C4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_14715</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C9C4"/>
    <dbReference type="OrthoDB" id="5989905at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8040429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="239" mass="27633" checksum="C5E3167191D2EADE" modified="2023-09-13" version="1">MFDIRELPDNQGVYEPLEGKIKRVIEQIQSKEVQTSRKILPAKLKVKVEWKFYVDKTSGLIKSGDFTGVEHHKIQSTIQLENIIPGHPKLSIIKDLWTQFSCIMTLLRGKGDFTTCQIEVLQKKCKDWVKLFTTVYLAKDVTPYMHVLVYHVPESLRIHGNINVYSQQSVEKLNDTVTCWFFRSSNHQNNSALKQVLCKQNRTDYLNTNCQRDRKFSVSCSACLGEGHNKRTCLKPKNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CAX2</accession>
    <name>A0A9Q1CAX2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">B(0,+)-type amino acid transporter 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_12214</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CAX2"/>
    <dbReference type="OrthoDB" id="3257095at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050598">
        <property type="entry name" value="AminoAcid_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785:SF512">
        <property type="entry name" value="SOBREMESA, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8041406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="100" mass="11092" checksum="3F7E8D6B4ADD0C95" modified="2023-09-13" version="1">MLYIFFEYSTGALCYAELGTMIPKSGGEYPYLMDTYGAPIAFLFSWVSIILTRPSSISIIALTFADYAVQPFFPADECEPPWNVGTDDNLKGGGQFQHKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CDW7</accession>
    <name>A0A9Q1CDW7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_10133</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CDW7"/>
    <dbReference type="OrthoDB" id="10068084at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="4">
        <location>
            <begin position="327"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="26"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="270"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="33"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8043161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="365" mass="40890" checksum="6D43A15C0E5ECBCA" modified="2023-09-13" version="1">MEDLEERVKQMELAYEARIKSLEEQLTKAKTVPEQQSPAVGQSSQPSTSQVTLGRKEFRVVGKIGERGQKDTLTFSNLVRQIELGVKKGYKDEEVIAAVISSILPGMTLRSYLEGKSGLTLPQLRRVIRVHFHEKDATELYIQLSKLTQSNRESPQDYLLKALDLKQKILFASREDQAPVTYGSGQVQAMFLHSLVTGLNNDSIKWEIKQLTQRAVPLTDEEILEEVNKVAKQETERQGKLKQKVQTSAAIEIATAKPDETEKIVAQKTKGKLQDEVTELKAEVKALKAALEQQEAQAQTLNRGRQRRSRGCDVCKAKGEGDGCDHCYKCGGNNHFARSCRVRRGQNQGNQEGFLLGDKEVPKTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CEJ9</accession>
    <name>A0A9Q1CEJ9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">E2 ubiquitin-conjugating enzyme</fullName>
            <ecNumber evidence="2">2.3.2.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_11175</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.</text>
            <dbReference type="EC" id="2.3.2.23"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="2"/>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CEJ9"/>
    <dbReference type="OrthoDB" id="9973183at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd23801">
        <property type="entry name" value="UBCc_UBE2L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000011">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="7">
        <location>
            <begin position="2"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="5">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8043877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="153" mass="17707" checksum="9A70F5E50EB76CF2" modified="2023-09-13" version="1">MAATRRLTKEMTEIRKSAMTCFQIEEIDSNILLWKGLIIPDSEPYNKGAFKIEMTFPAEYPFKPPKITFKTKIYHPNIDEKGQVCLPIIQPENWKPATKADQVIQALVALVQNPEPEHPLRGDLAEEYIKDVKRFNKNAEEYTKKYGEKRPAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CF36</accession>
    <name>A0A9Q1CF36_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_11682</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8044268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CF36"/>
    <dbReference type="OrthoDB" id="10048622at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8044268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="514" mass="58603" checksum="8D87654E6CA76584" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CFI8</accession>
    <name>A0A9Q1CFI8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Jerky protein-like-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_11012</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CFI8"/>
    <dbReference type="OrthoDB" id="10043687at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050863">
        <property type="entry name" value="CenT-Element_Derived"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004875">
        <property type="entry name" value="DDE_SF_endonuclease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19303:SF74">
        <property type="entry name" value="POGO TRANSPOSABLE ELEMENT WITH KRAB DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19303">
        <property type="entry name" value="TRANSPOSON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03184">
        <property type="entry name" value="DDE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE-1" evidence="2">
        <location>
            <begin position="192"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="372"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="372"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="385"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8043763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="400" mass="45193" checksum="EF50F5DB78EEBFFD" modified="2023-09-13" version="1">MKRAYVLVTADKMSIRKAAWQCGVPIQTLRDRLERGVNQARPLGGDPLLSRSEEEGLVKHIEYLAKCGYPLTRKDVINLATETAIFLKKRPQNAGNLSKRWFVRFMSRWPELRLAKAQKLSMKRAKATSDETVQSYFQELDRILTKYDIKNKPHLIYNVDETGFQTEHTPSKVVGPRGLKLNAITSERGPTTTLLACGNAVGTCLPPYFVFKGARYDARLMAGAVPGSKCSMSKSGWSNGNIFKHFLEEHFIPFLPQRKPDDHVLLLYDGHTSHISIPLIELAKEHKIILFVLPPHTSHVLQPLDVGLFKPLKTFFQNACGARMRERPGEVITRYDEPKSHPKKRKVSYRPGGVAITEETIILKIRKVYEEKQRKNGGEKKAGSTELNAEHISSNPDADV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CG94</accession>
    <name>A0A9Q1CG94_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_07545</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CG94"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8044722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="91" mass="10096" checksum="80D6DDEE2F84B668" modified="2023-09-13" version="1">MGELCRNCKKPNHFAKVCRTKKDKEQYSAGRNASSVQQMDNDDTTDGNEFFVGVMNTPGKPLKNNTRTVNLSLESKPVTVQIETGAQCNVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CGK3</accession>
    <name>A0A9Q1CGK3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase A2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_11732</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8044300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CGK3"/>
    <dbReference type="OrthoDB" id="10063781at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="CDD" id="cd05481">
        <property type="entry name" value="retropepsin_like_LTR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF7">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="145"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="169"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8044300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="337" mass="37951" checksum="7E3D794CB269AB22" modified="2023-09-13" version="1">MKQLPTENVEDFMAKCRIQADKCKFSTAESEQRLIEQLILGTAHKKVQEKLLGEDETLTVDKAMNIPRTCEATKAHVEAFTSATVSVDFINRKGQNKNLGSRSKADSQTKCRKCNLKYPHKGKCPAEGSTCNYCQKPNHWEAACRQKKRDNQGKHNRNRPRNRSRSRRERSSSRNQAEFREDRNRNIDMISLSRVDSSSDIEQDFESLSFDSITIRNMSNVKSNQESEASASIGIKVDGKPPTLRVKIDTGAEGNVIPMHVIKNFYPEFVNKTGKPLSKIAKPCDTRVTAYNGGTIPHYGTCTISCSYNNKHISTDFYIAEVQGPAILGLRDSIQLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CHS2</accession>
    <name>A0A9Q1CHS2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_08136</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CHS2"/>
    <dbReference type="OrthoDB" id="10447186at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8045186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="128" mass="14745" checksum="4D4059FEC2C0B35B" modified="2023-09-13" version="1">MEKRISSASLPSHVAKKMQDCPRYRLLLLKKAEVDLELKRRKQTIQTLAADVETLALEVEKAKLEEKWGLKKPGEKLKPAEESLRLCQLLDQNPDELDAILRELCIHQSKVTSQVTDLKMQLKQLEKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CHY8</accession>
    <name>A0A9Q1CHY8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">5'-AMP-activated protein kinase subunit beta-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_08802</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the 5'-AMP-activated protein kinase beta subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CHY8"/>
    <dbReference type="OrthoDB" id="10071715at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031588">
        <property type="term" value="C:nucleotide-activated protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02859">
        <property type="entry name" value="E_set_AMPKbeta_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032640">
        <property type="entry name" value="AMPK1_CBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050827">
        <property type="entry name" value="CRP1_MDG1_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10343">
        <property type="entry name" value="5'-AMP-ACTIVATED PROTEIN KINASE , BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10343:SF84">
        <property type="entry name" value="5'-AMP-ACTIVATED PROTEIN KINASE SUBUNIT BETA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16561">
        <property type="entry name" value="AMPK1_CBM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AMP-activated protein kinase glycogen-binding" evidence="5">
        <location>
            <begin position="371"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="11"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="68"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="92"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="124"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="157"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="180"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="221"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="260"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="327"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00025180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00040010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF16561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8045737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="452" mass="49020" checksum="8777B4D44CD1DED5" modified="2023-09-13" version="1">MGNKFGKKNQYDLSGNGQAEGSSVDKSGSPKKNSKRESYVGDDTVQVTAVKKTEDESKQAGGENQDAAVKEEAVEEEPKKDEAGEAAPAAAEEEKKDEKETKETTEPEKEVEEEKKEDDKPSTEPEPTPVEPEPTPTEPEPTPAEPEPTPAEPEPTPVETEPTPAEPESTPVEPESTPAEPEPTPTEPEPTPAEPEPTPEEPEQAPEKESEPVTEEPAPETSKDAETKDEVKEEDTVETAAAVAEEKADEPAAETGDAEEAAHQEESQEAGDAEKEEEKVETKEEHPEPETTQGSEQEEATQEDTSASKEEAPTQEETVPAALEESEKADEEEKVEETKDEVEASEETAPESKIEDATPDVVEGTSDSNKVPVEFRWEEGGSNVVVTGSFNDWAEQIPLEQSEGHFSVTLDLAEGEHKYKYIVDGEDKVDSSKPTVETEFGLNNVITVSKDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CI82</accession>
    <name>A0A9Q1CI82_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Sulfotransferase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_08911</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CI82"/>
    <dbReference type="OrthoDB" id="10014804at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48312">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48312:SF1">
        <property type="entry name" value="SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040298711" description="Sulfotransferase family protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8045817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="303" mass="35409" checksum="B6E85E011061CF55" modified="2023-09-13" version="1">MAHRENQVCVFLWALPRSVSTALTKCMSFVDGVQVWNEPYLYSHHNEKYQDPVALEKMAKVGMNFQDVEKILKPIIDSVPVGKRMKNALFTFSWVKNQLETQDPEKEVIFVKDMAHVLNNRYEYLPTARKFRHTFLIRHPSRMYPSFRKMMLATMSEDKRSAADIVLDVPLYEVHKIYKYAYDLWKYTQQNLDPNAIVIDGTELATYPEKLLPKYFEAVGISFKEKYLTWDPSPDIITDWKTALEPVVAVYHFGFIKSAVDSSHFITPDEKASSPDDMTPDVIKCIDESLPYYNEMFKHRIKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CIM3</accession>
    <name>A0A9Q1CIM3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">hypoxia-inducible factor-proline dioxygenase</fullName>
            <ecNumber evidence="9">1.14.11.29</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HOLleu_08238</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Nanhai2018</strain>
            <tissue evidence="15">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-prolyl-[hypoxia-inducible factor alpha subunit] + 2-oxoglutarate + O2 = trans-4-hydroxy-L-prolyl-[hypoxia-inducible factor alpha subunit] + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:48400"/>
            <dbReference type="Rhea" id="RHEA-COMP:12093"/>
            <dbReference type="Rhea" id="RHEA-COMP:12094"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:50342"/>
            <dbReference type="ChEBI" id="CHEBI:61965"/>
            <dbReference type="EC" id="1.14.11.29"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.29" evidence="9"/>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CIM3"/>
    <dbReference type="OrthoDB" id="5952526at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160082">
        <property type="term" value="F:hypoxia-inducible factor-proline dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031418">
        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071456">
        <property type="term" value="P:cellular response to hypoxia"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051559">
        <property type="entry name" value="HIF_prolyl_hydroxylases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006620">
        <property type="entry name" value="Pro_4_hyd_alph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044862">
        <property type="entry name" value="Pro_4_hyd_alph_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12907">
        <property type="entry name" value="EGL NINE HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12907:SF26">
        <property type="entry name" value="HIF PROLYL HYDROXYLASE, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13640">
        <property type="entry name" value="2OG-FeII_Oxy_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01753">
        <property type="entry name" value="zf-MYND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00702">
        <property type="entry name" value="P4Hc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144232">
        <property type="entry name" value="HIT/MYND zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50865">
        <property type="entry name" value="ZF_MYND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0847">Vitamin C</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MYND-type" evidence="13">
        <location>
            <begin position="26"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="14">
        <location>
            <begin position="418"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="230"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="254"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="265"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ8045264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="527" mass="58479" checksum="9D5EC2C31B9FB1D8" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CIY3</accession>
    <name>A0A9Q1CIY3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_04757</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CIY3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8046166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="98" mass="11304" checksum="7B95823B04B232E7" modified="2023-09-13" version="1">MKKATGKDTENLLDEQHLKRALCRAVTYTAFSSEMFPARTVTFKRLLVKRFAEDVSHRVQAEIRTCWQALKRNEGKVLAAMRDCVPCILNCYSGDHRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CJ23</accession>
    <name>A0A9Q1CJ23_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_04839</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CJ23"/>
    <dbReference type="OrthoDB" id="417598at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8046226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="188" mass="20771" checksum="004E8A037DD9C8D2" modified="2023-09-13" version="1">MSDDYELAHKSSFGRSRDQKSHKGFKKKDSGGKLESGKESDARGTPSGDDSRKPAKGRPTCRYCHKIGHTYETCFRRLRDLDKPTAFISSPPISVMDMGSQSVLSGSKELSTLFESSVSCSNVDYVRAKLKKHGYDGFISLGTVTRLDNNESSKVVILRDTCASRSLMLRSVLPLGREVDDSYRTCAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CJ30</accession>
    <name>A0A9Q1CJ30_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TOG array regulator of axonemal microtubules protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_08698</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CJ30"/>
    <dbReference type="OrthoDB" id="63891at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005881">
        <property type="term" value="C:cytoplasmic microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034085">
        <property type="entry name" value="TOG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21567">
        <property type="entry name" value="CLASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21567:SF87">
        <property type="entry name" value="CRESCERIN-LIKE PROTEIN CHE-12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21040">
        <property type="entry name" value="CEP104-like_TOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01349">
        <property type="entry name" value="TOG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TOG" evidence="2">
        <location>
            <begin position="61"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="TOG" evidence="2">
        <location>
            <begin position="333"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="615"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="632"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="653"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="665"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="688"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="716"/>
            <end position="727"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8045653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="753" mass="84908" checksum="B8224736EC0648C3" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CJP3</accession>
    <name>A0A9Q1CJP3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 19">Polypeptide N-acetylgalactosaminyltransferase</fullName>
            <ecNumber evidence="19">2.4.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Protein-UDP acetylgalactosaminyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">HOLleu_09075</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">Nanhai2018</strain>
            <tissue evidence="21">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-seryl-[protein] + UDP-N-acetyl-alpha-D-galactosamine = a 3-O-[N-acetyl-alpha-D-galactosaminyl]-L-seryl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23956"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:12788"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:53604"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:67138"/>
            <dbReference type="EC" id="2.4.1.41"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-threonyl-[protein] + UDP-N-acetyl-alpha-D-galactosamine = a 3-O-[N-acetyl-alpha-D-galactosaminyl]-L-threonyl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52424"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11689"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:67138"/>
            <dbReference type="ChEBI" id="CHEBI:87075"/>
            <dbReference type="EC" id="2.4.1.41"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 19">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 19">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 19">Golgi apparatus membrane</location>
            <topology evidence="2 19">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 19">Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="19"/>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CJP3"/>
    <dbReference type="OrthoDB" id="429263at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004653">
        <property type="term" value="F:polypeptide N-acetylgalactosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23441">
        <property type="entry name" value="beta-trefoil_Ricin_GALNT14-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02510">
        <property type="entry name" value="pp-GalNAc-T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000020">
        <property type="entry name" value="Polypeptide N-acetylgalactosaminyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045885">
        <property type="entry name" value="GalNAc-T"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675">
        <property type="entry name" value="N-ACETYLGALACTOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675:SF36">
        <property type="entry name" value="POLYPEPTIDE N-ACETYLGALACTOSAMINYLTRANSFERASE 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00652">
        <property type="entry name" value="Ricin_B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00458">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="Ricin B-like lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 19" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 19" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="13 19" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10 19" id="KW-0430">Lectin</keyword>
    <keyword evidence="16 19" id="KW-0464">Manganese</keyword>
    <keyword evidence="14 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="7 19" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="domain" description="Ricin B lectin" evidence="20">
        <location>
            <begin position="405"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00050905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00052209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU361242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM00458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAJ8045950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="534" mass="61150" checksum="706C56294FA402E9" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CL84</accession>
    <name>A0A9Q1CL84_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Contactin-5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_06671</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CL84"/>
    <dbReference type="OrthoDB" id="5982258at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098632">
        <property type="term" value="F:cell-cell adhesion mediator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000005">
        <property type="entry name" value="Neuronal cell adhesion molecule"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170:SF6">
        <property type="entry name" value="CONTACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170">
        <property type="entry name" value="PROTEIN SIDEKICK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Ig-like" evidence="9">
        <location>
            <begin position="37"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="9">
        <location>
            <begin position="146"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="9">
        <location>
            <begin position="228"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="9">
        <location>
            <begin position="322"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="290"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="413"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8047627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="458" mass="50099" checksum="777C6E7F92853C50" modified="2023-09-13" version="1">MGSAMEIAVWISITFVFLFKIGGALSSKAFLTLSEKPKDTVFNTDSIREFATFHCNAHGYPKPDITWLANDQIIRENGLYSVAEDGSLRISEPTRLLNGSTYRCIANNSFGAVMSEAALLEIAYVGQVLEVQLPDVSVNTRLGKCITCNPPNAYPGIKVTWVKETRWITPNERITVMMDGRLCFAHSEIGDTGNFHCLVSNRYDDGGAASFTRGSSVRFSVLLGGSNPPQSLTPVTGIQESVTVRQGQEAVLECFFLGSPTPTLSWSRRGNGLPTNSTMSRHNQTLRIPNAQPEDGGEYECTGETSDGDSETVYRRLTVLAPPFLVSGLEDEEVEPGRNVTLQCTADSDAVEFSWYHNARLINSSMQRPRISEDTTLTINNAMVEDSGIYQCAARNEHGMTLSTAQLFVKEQDPTEENPVESEINQPNVESPGNGAGNTLVHIFSTVVMVSAVLFEVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CLI9</accession>
    <name>A0A9Q1CLI9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_06467</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CLI9"/>
    <dbReference type="OrthoDB" id="10066002at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31025">
        <property type="entry name" value="SI:CH211-196P9.1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31025:SF9">
        <property type="entry name" value="SI:DKEY-286J15.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8047463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="201" mass="23224" checksum="2540C1D7763286B0" modified="2023-09-13" version="1">MKEWLCHNIDPEAKVKEFMEKTAVKREALLRDENFTLDQIIKEFPRLFDTPGMVEVDFRTVFPDVADRLYMQWTLGFSEKVLQYATLQEKWENYLNVNSEAINTDKKKVNASLSVLPVTLPCGKKGRKKATIMDAKKSYMDVQPQIVTNIPKYLDEVTNRQPYILVLGEALDPSQVFVIVERKALEQASLLKAVDVCFKLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CNI9</accession>
    <name>A0A9Q1CNI9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_01051</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CNI9"/>
    <dbReference type="OrthoDB" id="1743609at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8048652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="178" mass="20629" checksum="5F56C513C21A45DD" modified="2023-09-13" version="1">MVWLRFTRVTWNEKVDLVEARLKDFGDRYISLLGKVTVINRFVFPLLWYPGTVLAAPEGVLVRLERAVFRFPWKAKTDLVRRKVVYQVPRQGGLSLVHFPSKLRFLLTKCVCSAVDRQMPFAYFVRYRGGLSFRWFFPTLFSNSVPHSVLSDEGNNTYYVNCRGRFAVQCGGPVPGHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CNV8</accession>
    <name>A0A9Q1CNV8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Membrane progestin receptor gamma-A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_00615</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ADIPOR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CNV8"/>
    <dbReference type="OrthoDB" id="529367at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004254">
        <property type="entry name" value="AdipoR/HlyIII-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20855">
        <property type="entry name" value="ADIPOR/PROGESTIN RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20855:SF141">
        <property type="entry name" value="MEMBRANE PROGESTIN RECEPTOR GAMMA-B-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03006">
        <property type="entry name" value="HlyIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="47"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="76"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="113"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="148"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="178"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="288"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="249"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="253"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR604254-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8048340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="339" mass="39373" checksum="CB1D8CB5A53F2A16" modified="2023-09-13" version="1">MWTVKLLKADHVPSDFREPFIINGYRSCRCTVSTCLFSALQYTNETMNFWTHFAGFLFFLWQMYQTGDRYWKYDDPFTWAFIAYLISCCIYLVTSSWAHLFSCMSEWFRHVCFFMDYGALSVYSFGCALAYNYYVFPTDYLGTWAHKLFVPLAFVNGLCCTMLACSSRLMHEGKLRKIFRMVAFMLPYVCCSVPLLYRMVFCQGMECEAESFVAHKWQFYFAFITSFLYASHFPEVLAPGKFDFIGHSHQIFHVCGCLATYSQFLAVVSDMCTRRELLLAHAPSGTFMSTLGLLGLLVVSNLLIIASFANYIWSPEQLRKIKFMQGKKTIISFDEKKSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CQC9</accession>
    <name>A0A9Q1CQC9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL11</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">60S ribosomal protein L12</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_01756</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the universal ribosomal protein uL11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CQC9"/>
    <dbReference type="OrthoDB" id="1478556at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00349">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.250:FF:000002">
        <property type="entry name" value="60S ribosomal protein L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1550.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.250">
        <property type="entry name" value="Ribosomal protein L11, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1550.10">
        <property type="entry name" value="Ribosomal protein L11/L12, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00736">
        <property type="entry name" value="Ribosomal_uL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000911">
        <property type="entry name" value="Ribosomal_uL11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020783">
        <property type="entry name" value="Ribosomal_uL11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036769">
        <property type="entry name" value="Ribosomal_uL11_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020785">
        <property type="entry name" value="Ribosomal_uL11_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020784">
        <property type="entry name" value="Ribosomal_uL11_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036796">
        <property type="entry name" value="Ribosomal_uL11_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00298">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03946">
        <property type="entry name" value="Ribosomal_L11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00649">
        <property type="entry name" value="RL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54747">
        <property type="entry name" value="Ribosomal L11/L12e N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46906">
        <property type="entry name" value="Ribosomal protein L11, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00359">
        <property type="entry name" value="RIBOSOMAL_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6 9" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL11 N-terminal" evidence="8">
        <location>
            <begin position="13"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL11 C-terminal" evidence="7">
        <location>
            <begin position="74"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8049146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="165" mass="17934" checksum="2A86C31DF13D873C" modified="2023-09-13" version="1">MPPKFDPNAITIVYLRAVGGEVGATSALAPKIGPLGLSPKKVGDDIAKGTQDWKGLKITVKLTIQNRQAKVSVVPSASSLIIKALKEAPRDRKKVKHVQHNGNITMDDIINIAREMRERSMARKLEGTVKEILGTAQSVGCTVDKEPPHDIIDKINEGEIEIPEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CQU2</accession>
    <name>A0A9Q1CQU2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_01300</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CQU2"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8048829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="176" mass="20046" checksum="2E372F358966FE0B" modified="2023-09-13" version="1">MEVDESPVAQKPVEVFRFLGKPNQQLMDEPMTHFPLRDIENSSVTTKDVPPVVVQILEFIFATMMDKVTYGYLKQNCEGCDVGWPSQLDYRCLNFGSCMPVSDMYFNDMQHLVKDAWVVETARDYLLNVTETPILNAVMNNIRTSWKASPQKAVDALEKTDLGRKYSPSFGGRLHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CSE3</accession>
    <name>A0A9Q1CSE3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">asparaginase</fullName>
            <ecNumber evidence="1">3.5.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_04031</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.1" evidence="1"/>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CSE3"/>
    <dbReference type="OrthoDB" id="542841at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004067">
        <property type="term" value="F:asparaginase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009066">
        <property type="term" value="P:aspartate family amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.40:FF:000001">
        <property type="entry name" value="L-asparaginase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1170">
        <property type="entry name" value="L-asparaginase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036152">
        <property type="entry name" value="Asp/glu_Ase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006034">
        <property type="entry name" value="Asparaginase/glutaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027475">
        <property type="entry name" value="Asparaginase/glutaminase_AS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040919">
        <property type="entry name" value="Asparaginase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027473">
        <property type="entry name" value="L-asparaginase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027474">
        <property type="entry name" value="L-asparaginase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037152">
        <property type="entry name" value="L-asparaginase_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11707:SF28">
        <property type="entry name" value="60 KDA LYSOPHOSPHOLIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11707">
        <property type="entry name" value="L-ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00710">
        <property type="entry name" value="Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17763">
        <property type="entry name" value="Asparaginase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001220">
        <property type="entry name" value="L-ASNase_gatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500176">
        <property type="entry name" value="L_ASNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00139">
        <property type="entry name" value="ASNGLNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00870">
        <property type="entry name" value="Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53774">
        <property type="entry name" value="Glutaminase/Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00917">
        <property type="entry name" value="ASN_GLN_ASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51732">
        <property type="entry name" value="ASN_GLN_ASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="L-asparaginase N-terminal" evidence="4">
        <location>
            <begin position="9"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="Asparaginase/glutaminase C-terminal" evidence="5">
        <location>
            <begin position="241"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF17763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8050722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="367" mass="40916" checksum="3044C3DE31147112" modified="2023-09-13" version="1">MNTDFSPDVLVLLTGGETTMKKNERGVYEIVPLETLRILMSNLQTHDSDFAKKKNLKDTLFALPPSESGDRIIYKVKEYTELKDSSNISIEDWEKIAQDIKNEYERYKGFVVLHGTDTIAYTASALSFMMHGLTKPVILTTSRFPVSEQTSKCFDNLINALNICCYYQIQEQQVAILYEKVLFRGNRVTIFSSDDQVNFESPNYPPLAYENTFRTSPSTERNLTPNDETRHVQFQTTMSKNVGILRLFPGVTSRLINNFMNSGLEGIVLQTYGAGNIASENKEVLEEIRLACTQGAIIVNCSQCSRGSVSLTYATNTGLQGVGVLSGGDMTTEAAFAKLSFVLAQEGLSLEEKKRMMITNISGELSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CSN6</accession>
    <name>A0A9Q1CSN6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_03517</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CSN6"/>
    <dbReference type="OrthoDB" id="5411773at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8050350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="111" mass="12962" checksum="4EDF4A42F891B117" modified="2023-09-13" version="1">MDVLNSWADVPDMEDVTFLEYTRQWINVQSRGGLFKVSDDVYRFFRLMEFVTKAEVNTNNIGTLKKTNIHALLLTALRNDKYVMKTWDKIRSKLSTELGNLLLTKSHDMLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1GZT4</accession>
    <name>A0A9Q1GZT4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">2-hydroxyacylsphingosine 1-beta-galactosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_29595</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8030027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1GZT4"/>
    <dbReference type="OrthoDB" id="5835829at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008194">
        <property type="term" value="F:UDP-glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000021">
        <property type="entry name" value="UDP-glucuronosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000205">
        <property type="entry name" value="UDP-glucuronosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050271">
        <property type="entry name" value="UDP-glycosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043">
        <property type="entry name" value="EG:EG0003.4 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043:SF145">
        <property type="entry name" value="FI06409P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040305031" evidence="5">
        <location>
            <begin position="27"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="494"/>
            <end position="517"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8030027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="547" mass="62098" checksum="99BB18360C8DDA06" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H244</accession>
    <name>A0A9Q1H244_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_01919</name>
        <name evidence="2" type="ORF">HOLleu_25879</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H244"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8032367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8049250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="57" mass="6442" checksum="C89C71BF19D3FD4D" modified="2023-09-13" version="1">MRRGHRVVREGARRQGKHKGVWEGEELGGDRTGLCGRGLGTFGQDIEVEIEYILFKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H357</accession>
    <name>A0A9Q1H357_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_23835</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H357"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="398"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="182"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="76"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="187"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8033552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="427" mass="49186" checksum="0AED666D7DE03C65" modified="2023-09-13" version="1">MRCGRGLPSYRDPAPVSQDDDSCDKLFKLKHCREKLHDQYYGLVYRVWQWNNLEEGLSSVMELISHSESGNGEHLKDSSKKRRESLEEMMANWERSFDEKINDEEFSALFAKCKEVLTNLKETLNNDDVSLTPIQNKIEELIVLANIQRQYLSKQCGNVETHKVCSALQNLYKIFSSKTQEQGLTESDSKPAEKQNEKKKKGKDTASSIHSDLKQIVDFVCQKQSFVQKLEKIFPPEGTENIRVPNDVIQWAKACVEAHGALIELSDIKELEKTTELYKTVKELFDNECAKVEAQQKIEKIMEGFTANFADFFAKVMLLIGEIDEHLQVKKKPQDSSKKLKKTLEDAKDRLIQECHKKVNYLREERINLVSFVYYSSLQCNSIKARCCLFDSLPKAEGIYELVMSCVWVCVFVYVMLLLVNTISQEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H3N0</accession>
    <name>A0A9Q1H3N0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_27671</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8031066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H3N0"/>
    <dbReference type="OrthoDB" id="2113965at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8031066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="174" mass="19064" checksum="AAB3FE37DDD07F55" modified="2023-09-13" version="1">MPHIERKNPIVFGGDQRSPGVTGGQNLKTLLTQYLEMRYGKRKNPIIFGGGQRSPGVTRGQTMKTLLTQYLKMRYGKRKNPIIFGGGERSSGVTGGQTLKTLLTRYLKKGNSDTEEPYCFGGGQRSPGVTGGQTLKTLLTQYLEMSYGKRKNPIIFGGGERSSGVTRGQTLKTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H4B2</accession>
    <name>A0A9Q1H4B2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SCAN box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_25897</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H4B2"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF13">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCAN box" evidence="2">
        <location>
            <begin position="233"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="327"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="327"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="342"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="366"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8032383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="559" mass="63112" checksum="08CA5EF7B3A02462" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H626</accession>
    <name>A0A9Q1H626_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_17539</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8036882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H626"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF13">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8036882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="172" mass="20810" checksum="75E745BF0CD8EF86" modified="2023-09-13" version="1">MRKDEIKKHVLEYLVDESILPSTVLETAITVPTDNTFELKRLEIEMNKEIRLKEMEREREKKEEREMQMQKEREEREERERREAREHEFRLKQLELGVIKASDPKIGLDTHVKFVPKFQEDNVEKFFNHFEKLGEQLKWPRDKWSILIQSNFTGKAQEVYSTLSIRGQHGLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H7E9</accession>
    <name>A0A9Q1H7E9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Low-density lipoprotein receptor-related protein 8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_15873</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H7E9"/>
    <dbReference type="OrthoDB" id="6369184at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000005">
        <property type="entry name" value="Procollagen C-endopeptidase enhancer 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050778">
        <property type="entry name" value="Cueball_EGF_LRP_Nidogen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003410">
        <property type="entry name" value="HYR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000033">
        <property type="entry name" value="LDLR_classB_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46513">
        <property type="entry name" value="VITELLOGENIN RECEPTOR-LIKE PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02494">
        <property type="entry name" value="HYR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00058">
        <property type="entry name" value="Ldl_recept_b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00135">
        <property type="entry name" value="LY"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63825">
        <property type="entry name" value="YWTD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50825">
        <property type="entry name" value="HYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51120">
        <property type="entry name" value="LDLRB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040355668" evidence="5">
        <location>
            <begin position="25"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="6">
        <location>
            <begin position="30"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="4">
        <location>
            <begin position="160"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="4">
        <location>
            <begin position="204"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="HYR" evidence="7">
        <location>
            <begin position="359"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8038442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="515" mass="57153" checksum="F1A78D460DE6C7ED" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H7Y3</accession>
    <name>A0A9Q1H7Y3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phospholipase A2</fullName>
            <ecNumber evidence="7">3.1.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_16629</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1-acyl-sn-glycero-3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15801"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="4">Binds 1 Ca(2+) ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the phospholipase A2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.4" evidence="7"/>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H7Y3"/>
    <dbReference type="OrthoDB" id="5841574at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047498">
        <property type="term" value="F:calcium-dependent phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050482">
        <property type="term" value="P:arachidonate secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.90.10">
        <property type="entry name" value="Phospholipase A2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001211">
        <property type="entry name" value="PLA2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016090">
        <property type="entry name" value="PLA2-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033113">
        <property type="entry name" value="PLA2_histidine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036444">
        <property type="entry name" value="PLipase_A2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11716:SF100">
        <property type="entry name" value="PHOSPHOLIPASE A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11716">
        <property type="entry name" value="PHOSPHOLIPASE A2 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00068">
        <property type="entry name" value="Phospholip_A2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00389">
        <property type="entry name" value="PHPHLIPASEA2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00085">
        <property type="entry name" value="PA2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48619">
        <property type="entry name" value="Phospholipase A2, PLA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00118">
        <property type="entry name" value="PA2_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0106">Calcium</keyword>
    <keyword evidence="3 5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Phospholipase A2-like central" evidence="8">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="20"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR601211-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR601211-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8039042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="82" mass="9448" checksum="C75DE3DCAF307E8F" modified="2023-09-13" version="1">MISCMNDRSWYHYNGYGCWCGIGGLGQPLDATDQCCKDHDDCYDNLVDTGACLWSWQLYVVEYIGWCRNRGNPWVTNHIECG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H7Y6</accession>
    <name>A0A9Q1H7Y6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_20365</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H7Y6"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8036404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="79" mass="9269" checksum="BBBC14882556677E" modified="2023-09-13" version="1">MKHSQLPWTPRDCPWDPDWHLDPTRSPLDVMAMQEGLSAATEPLAQTDQLLTNTKCNLLLCNFHSYKLTILEYYQHNHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H858</accession>
    <name>A0A9Q1H858_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_19959</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H858"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8036090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="109" mass="12575" checksum="6482206CB6F9C5EE" modified="2023-09-13" version="1">MPLLSTVTLQSINDLSFFSRLSNPLLYLVGGTSCTRKWIDNKRGGGHKENYPDYLQDDEKRAMLQLEDATRKFLAILLKEGRQEKLKSGGPNQNVYGLSSYDYDFQRYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H862</accession>
    <name>A0A9Q1H862_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Kynurenine formamidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_16924</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H862"/>
    <dbReference type="OrthoDB" id="433474at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004061">
        <property type="term" value="F:arylformamidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050300">
        <property type="entry name" value="GDXG_lipolytic_enzyme"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081">
        <property type="entry name" value="AB HYDROLASE SUPERFAMILY PROTEIN C4A8.06C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081:SF33">
        <property type="entry name" value="KYNURENINE FORMAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="2">
        <location>
            <begin position="142"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8039267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="391" mass="44066" checksum="75C4639EEC641A16" modified="2023-09-13" version="1">MEKGYNFTKDDKGIPCVYHEWQSLNGETLGNLFSPSKYSRSGDDPDQVIEQYVKNTDERSIETRSSLKGELNVSVGGNTRQTVDLFIPENVSQDAPVLVFYHGGYWQFLSQVDKISDSEKAFSFIPRIKNQEVKYLYKLYDSKESHSMVARHLVPLGAIVAVAGYTIAPKGSVTQMIQECKEAVAFCARRYPKSRGIFVSGHSAGGHLAAMVLTTDWEKEFGLPSNLIKGACPLSGVFDLRPLVTTYINEPLHLTSTSALESSPVAHIPKIVANSKHCKIRCLYGDHEPPSFEIMCIQFCQLLREEGMDISCQKVKDADHFELFLSVWEEHSVVTKKVLYELLGKKRKRERSDPTIKVGFSYLAEKKSICSSRVAFSRGKMKKERNDEKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H942</accession>
    <name>A0A9Q1H942_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ataxin-2-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_17954</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H942"/>
    <dbReference type="OrthoDB" id="2275718at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040168179" evidence="1">
        <location>
            <begin position="16"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8037200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="324" mass="37255" checksum="A2B336B301F4815B" modified="2023-09-13" version="1">MFFFSIFSMLLIAESLLVHLWETWTKTKDALSVRNATTGSAHEEDEDDESAYHTDLAKEYCHCRTWRKEHSMLMSAFSKRRKLARTIKVSAADLKNLQGIYKTSLGCIRKSRYSTKGSDTCDVDVGIKRSDIDDFVKTTGQQRPRELVTGGDEHLNKEIRNPKAEAYDSDDVQRKTAKNSFPLLSEDRSGRAKQSTNVTEPCLSSRGFLTDKEIAATIPFQPKGPRSLIPFRCFVDDDLDYDMDALEGEGWNSEEMFEVNEIKFGYRSTYDEDLTEYTPILNVESTKTFRQRWLMACLLADEIKASKYRGEQLESKKKRKKVHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HA18</accession>
    <name>A0A9Q1HA18_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Sushi, von Willebrand factor type A, EGF and pentraxin domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_15599</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HA18"/>
    <dbReference type="OrthoDB" id="9991441at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051277">
        <property type="entry name" value="SEZ6_CSMD_C4BPB_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45656">
        <property type="entry name" value="PROTEIN CBR-CLEC-78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45656:SF4">
        <property type="entry name" value="PROTEIN CBR-CLEC-78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0768">Sushi</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040338382" evidence="5">
        <location>
            <begin position="21"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="6">
        <location>
            <begin position="67"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="6">
        <location>
            <begin position="178"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="6">
        <location>
            <begin position="242"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="6">
        <location>
            <begin position="437"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="97"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="208"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8038235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="503" mass="55314" checksum="DA2DF8BF51FFD369" modified="2023-09-13" version="1">MLLTKATFYVLIIIGNIVTASTPECLSRDVVAAGRPCARACSQDGTCRKRNKECVCDGLCGLSCLRKRCPTLTVPLNGNMVVNPPVRTIGAEVTYSCNDGYVLSGQRNRICQANKTWSGTEPMCTDSTTEVPSQECAERDTAAAGRNCRKACQPDGTCIKDNRECMCDGLCGLTCLKKRCPPLPTPRNGSFVINPDDRIIGAEVTYSCNNGYFISGRENRICQANKTWSGTQPTCTQPTTEVPCPILDLEDGALAYLDNNNQRIFEPHHGDIRVAVCNDGRLLRGTSQTRCISGSWQHALPSCEVEPCSVASLERYNMDIQYAVPPGSNEIEAGLKPAGTTANFICRQYGFEASRTKRCERGRWRGADPVCLRIPCDDELLENGDFTYTLNGVQYEDPVHGVVRSYNCYAGYTVEDDRPSQCDAGSWTRGLSNCIEIRCPTTQLDNGVLEYRDIDSRVTLDPKHTDFRIATCNTGYDLQGAEETQCLVGEWEDELPVCLEGPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HB52</accession>
    <name>A0A9Q1HB52_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_13663</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8039614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HB52"/>
    <dbReference type="OrthoDB" id="5989141at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1820">
        <property type="entry name" value="L1 transposable element, RRM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004244">
        <property type="entry name" value="Transposase_22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11505">
        <property type="entry name" value="L1 TRANSPOSABLE ELEMENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8039614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="226" mass="25951" checksum="CBBD7D337121787F" modified="2023-09-13" version="1">MPTRGRPSLSQKNSARDKPSATPHRPTQREGTVSSLVDQLADDNPSWEAALDNFKKVVDESVSSLHDGINQLKVDLGQAIEFQAKRIDDLESKMADILSDHTNFHAKISRLESALEQKDEELNKLERMSRRNNFRIVGYPKLHQENVEDIVRDEIIPLFGDSNGIVVERCHRDGRGFNGRPPHILVRCLSYRMKVNIMKNRRTVLYGKDYFIVDDLTKMDLAERKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HBG3</accession>
    <name>A0A9Q1HBG3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HOLleu_14107</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Nanhai2018</strain>
            <tissue evidence="18">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">In the C-terminal section; belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8039944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HBG3"/>
    <dbReference type="OrthoDB" id="2011769at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00878">
        <property type="entry name" value="Arf_Arl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19773">
        <property type="entry name" value="Bbox2_TRIM23_C-IX_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19774">
        <property type="entry name" value="Bbox2_TRIM23_C-IX_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000130">
        <property type="entry name" value="E3 ubiquitin-protein ligase TRIM23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000486">
        <property type="entry name" value="E3 ubiquitin-protein ligase TRIM23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003649">
        <property type="entry name" value="Bbox_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11711">
        <property type="entry name" value="ADP RIBOSYLATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00502">
        <property type="entry name" value="BBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="16">
        <location>
            <begin position="42"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="17">
        <location>
            <begin position="135"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="118"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="424"/>
            <end position="431"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="431"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="448"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="470"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="526"/>
            <end position="530"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00061142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ8039944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="588" mass="66523" checksum="C4107AC863EF272B" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HC88</accession>
    <name>A0A9Q1HC88_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_14145</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8039976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HC88"/>
    <dbReference type="OrthoDB" id="430238at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000020">
        <property type="entry name" value="Transposon Tf2-6 polyprotein-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041577">
        <property type="entry name" value="RT_RNaseH_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051320">
        <property type="entry name" value="Viral_Replic_Matur_Polypro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33064">
        <property type="entry name" value="POL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33064:SF37">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17919">
        <property type="entry name" value="RT_RNaseH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="122"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="122"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8039976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="215" mass="24675" checksum="240B4A0BD5BB0E14" modified="2023-09-13" version="1">MAFTEVLVYLEDLLVFSRTLEEHVEKIDKVLTRLKEFGLKLNPDKCSFLRSSVKCLGHVISAEGVQTDPDKVAAVKTWPHPQNVEELKSFHGFAGYYRRFIQDYSKIAKPLNDLSVLYEPLRRKRRQHRNQKREPGRHPPPNTPFGEHWREECQVACDTLLEKLTTAPTLHFANFEERFILHTDASTLGLGSALYQEHEGQAEASSLCEQGSVQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HCE0</accession>
    <name>A0A9Q1HCE0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">S-crystallin SL11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_11556</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8044172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HCE0"/>
    <dbReference type="OrthoDB" id="414243at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03192">
        <property type="entry name" value="GST_C_Sigma_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03039">
        <property type="entry name" value="GST_N_Sigma_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000030">
        <property type="entry name" value="Glutathione S-transferase S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000035">
        <property type="entry name" value="hematopoietic prostaglandin D synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050213">
        <property type="entry name" value="GST_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11571">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11571:SF150">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14497">
        <property type="entry name" value="GST_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13417">
        <property type="entry name" value="GST_N_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="1">
        <location>
            <begin position="2"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="2">
        <location>
            <begin position="81"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8044172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="211" mass="24630" checksum="CD249DCEA00707E5" modified="2023-09-13" version="1">MPSYKLISFDGKALAEVVRYLMELKGIKYEDRRIQKEAWPDLKPKTPLGQLPVLEIDGIQYPHSHAMYRYLAREYDFYGSSSKEQLEIDVVCETVDDIWPEFYKIFNAEGDEKKNELRKKLADDGAMKLITNLEKLLKKNNNGSGWFIGKKVTLADVMAFNIIYDFLPAVLEIKEGEFDLKEHDVLKAFVDRFKAQDKIADWIKRRPKTPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HCU0</accession>
    <name>A0A9Q1HCU0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phospholipase B1, membrane-associated</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Lysophospholipase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Phospholipase A2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Phospholipase B/lipase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Triacylglycerol lipase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="44" type="ORF">HOLleu_07477</name>
    </gene>
    <organism evidence="44 45">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="44" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="44">Nanhai2018</strain>
            <tissue evidence="44">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="20">Calcium-independent membrane-associated phospholipase that catalyzes complete diacylation of phospholipids by hydrolyzing both sn-1 and sn-2 fatty acyl chains attached to the glycerol backbone (phospholipase B activity). Has dual phospholipase and lysophospholipase activities toward diacylphospholipids. Preferentially cleaves sn-2 ester bonds over sn-1 bonds. Acts as a lipase toward glycerolipid substrates. Hydrolyzes fatty acyl chains of diacylglycerols with preference for the sn-2 position and of triacylglycerols with not positional selectivity. May also hydrolyze long chain retinyl esters such as retinyl palmitate. May contribute to digestion of dietary phospholipids, glycerolipids and retinoids, facilitating lipid absorption at the brush border.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="32">
            <text>1,2,3-tri-(9Z-octadecenoyl)-glycerol + H2O = di-(9Z)-octadecenoylglycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38575"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:53753"/>
            <dbReference type="ChEBI" id="CHEBI:75945"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="32">
            <dbReference type="Rhea" id="RHEA:38576"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + H2O = 1-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40923"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28610"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:74669"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="28">
            <dbReference type="Rhea" id="RHEA:40924"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="40">
            <text>1,2-dihexadecanoyl-sn-glycero-3-phosphocholine + 2 H2O = sn-glycerol 3-phosphocholine + 2 hexadecanoate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:40975"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:72999"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="40">
            <dbReference type="Rhea" id="RHEA:40976"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>1,2-dihexadecanoyl-sn-glycero-3-phosphocholine + H2O = 1-hexadecanoyl-sn-glycero-3-phosphocholine + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41223"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:72998"/>
            <dbReference type="ChEBI" id="CHEBI:72999"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="29">
            <dbReference type="Rhea" id="RHEA:41224"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="41">
            <text>1,3-di-(9Z-octadecenoyl)-glycerol + H2O = 1-(9Z-octadecenoyl)-glycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:39939"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:75342"/>
            <dbReference type="ChEBI" id="CHEBI:75735"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="41">
            <dbReference type="Rhea" id="RHEA:39940"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="37">
            <text>1,3-dihexadecanoyl-2-(9Z-octadecenoyl)glycerol + H2O = 1,3-dihexadecanoylglycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40983"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:75688"/>
            <dbReference type="ChEBI" id="CHEBI:77619"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="37">
            <dbReference type="Rhea" id="RHEA:40984"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>1,3-dihexadecanoyl-2-(9Z-octadecenoyl)glycerol + H2O = 1-hexadecanoyl-2-(9Z-octadecenoyl)-glycerol + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40979"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:75585"/>
            <dbReference type="ChEBI" id="CHEBI:75688"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:40980"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>1-(9Z-octadecenoyl)-glycerol + H2O = glycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38487"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:75342"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:38488"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="38">
            <text>1-O-hexadecyl-2-(9Z)-octadecenoyl-sn-glycero-3-phosphocholine + H2O = 1-O-hexadecyl-sn-glycero-3-phosphocholine + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40915"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:34112"/>
            <dbReference type="ChEBI" id="CHEBI:64496"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="38">
            <dbReference type="Rhea" id="RHEA:40916"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>1-hexadecanoyl-2-(9Z)-octadecenoyl-3-octadecanoyl-sn-glycerol + H2O = 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycerol + octadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41111"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:25629"/>
            <dbReference type="ChEBI" id="CHEBI:75466"/>
            <dbReference type="ChEBI" id="CHEBI:77623"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:41112"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="39">
            <text>1-hexadecanoyl-2-(9Z)-octadecenoyl-3-octadecanoyl-sn-glycerol + H2O = 1-hexadecanoyl-3-octadecanoyl-sn-glycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41103"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:77623"/>
            <dbReference type="ChEBI" id="CHEBI:77624"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="39">
            <dbReference type="Rhea" id="RHEA:41104"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>1-hexadecanoyl-2-(9Z)-octadecenoyl-3-octadecanoyl-sn-glycerol + H2O = 2-(9Z-octadecenoyl)-3-octadecanoyl-sn-glycerol + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41107"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:75558"/>
            <dbReference type="ChEBI" id="CHEBI:77623"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:41108"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine + H2O = (9Z,12Z)-octadecadienoate + 1-hexadecanoyl-sn-glycero-3-phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40811"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30245"/>
            <dbReference type="ChEBI" id="CHEBI:72998"/>
            <dbReference type="ChEBI" id="CHEBI:73002"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:40812"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="30">
            <text>1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine + H2O = 2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40971"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:73002"/>
            <dbReference type="ChEBI" id="CHEBI:76084"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="30">
            <dbReference type="Rhea" id="RHEA:40972"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phospho-(1'-sn-glycerol) + H2O = 1-hexadecanoyl-sn-glycero-3-phospho-(1'-sn-glycerol) + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40919"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:72841"/>
            <dbReference type="ChEBI" id="CHEBI:75158"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:40920"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="36">
            <text>1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + H2O = 1-hexadecanoyl-sn-glycero-3-phosphocholine + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38779"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:72998"/>
            <dbReference type="ChEBI" id="CHEBI:73001"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="36">
            <dbReference type="Rhea" id="RHEA:38780"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="34">
            <text>1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine + H2O = 1-hexadecanoyl-sn-glycero-3-phosphoethanolamine + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40911"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:73004"/>
            <dbReference type="ChEBI" id="CHEBI:73007"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="34">
            <dbReference type="Rhea" id="RHEA:40912"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="35">
            <text>1-hexadecanoyl-sn-glycero-3-phosphocholine + H2O = sn-glycerol 3-phosphocholine + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40435"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:72998"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="35">
            <dbReference type="Rhea" id="RHEA:40436"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="31">
            <text>1-octadecanoyl-2-(9Z,12Z)-octadecadienoyl-sn-glycerol + H2O = 1-octadecanoyl-sn-glycerol + (9Z,12Z)-octadecadienoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40927"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30245"/>
            <dbReference type="ChEBI" id="CHEBI:75550"/>
            <dbReference type="ChEBI" id="CHEBI:77097"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="31">
            <dbReference type="Rhea" id="RHEA:40928"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>2,3-di-(9Z)-octadecenoyl-sn-glycerol + H2O = 3-(9Z-octadecenoyl)-sn-glycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:75824"/>
            <dbReference type="ChEBI" id="CHEBI:75938"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:42605"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="42">
            <text>2-(9Z-octadecenoyl)-glycerol + H2O = glycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38491"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:73990"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="42">
            <dbReference type="Rhea" id="RHEA:38492"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1-acyl-sn-glycero-3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15801"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.4"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:15802"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>a 1-O-alkyl-2-acyl-sn-glycero-3-phosphocholine + H2O = a 1-O-alkyl-sn-glycero-3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36231"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:30909"/>
            <dbReference type="ChEBI" id="CHEBI:36702"/>
            <dbReference type="EC" id="3.1.1.4"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:36232"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="33">
            <text>a 1-acyl-sn-glycero-3-phosphocholine + H2O = sn-glycerol 3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15177"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.5"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="33">
            <dbReference type="Rhea" id="RHEA:15178"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12044"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17855"/>
            <dbReference type="ChEBI" id="CHEBI:18035"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="EC" id="3.1.1.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:12045"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 'GDSL' lipolytic enzyme family. Phospholipase B1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="44">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HCU0"/>
    <dbReference type="OrthoDB" id="10265800at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031526">
        <property type="term" value="C:brush border membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050253">
        <property type="term" value="F:retinyl-palmitate esterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01824">
        <property type="entry name" value="Phospholipase_B_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1110:FF:000005">
        <property type="entry name" value="Phospholipase B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035547">
        <property type="entry name" value="Phospholipase_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038885">
        <property type="entry name" value="PLB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21325:SF31">
        <property type="entry name" value="GH22081P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21325">
        <property type="entry name" value="PHOSPHOLIPASE B, PLB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="45" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 43" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="43">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040390228" description="Phospholipase B1, membrane-associated" evidence="43">
        <location>
            <begin position="19"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00029723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00031182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00031485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00033022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00045916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00047324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00047438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00047459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00048011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00048015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00048049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00048058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00048227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00048362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00048374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00048386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="ARBA" id="ARBA00048454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="ARBA" id="ARBA00048613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="35">
        <source>
            <dbReference type="ARBA" id="ARBA00048656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="36">
        <source>
            <dbReference type="ARBA" id="ARBA00048699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="37">
        <source>
            <dbReference type="ARBA" id="ARBA00048869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="38">
        <source>
            <dbReference type="ARBA" id="ARBA00048872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="39">
        <source>
            <dbReference type="ARBA" id="ARBA00048939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="40">
        <source>
            <dbReference type="ARBA" id="ARBA00049363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="41">
        <source>
            <dbReference type="ARBA" id="ARBA00049372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="42">
        <source>
            <dbReference type="ARBA" id="ARBA00049461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="43">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="44">
        <source>
            <dbReference type="EMBL" id="KAJ8044682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="45">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="437" mass="48719" checksum="271B076EC5FB47F9" modified="2023-09-13" version="1">MKLLLLLSVLCVSVLIQASQQDVDRYLDAYRAIITPKDNDTEPVPGFLEQLPGYECSQQPVWPGETRFSFPCAPLAKSAKKPTSVHYLRPGDVDLIGALGDSLSAANGADACFLPELAYMYRGRCFSSGADGSYATNPTLANILLKYNPDLKGYSLRTNWWTVPEAGFNVAIPGAVADDMPEQARAIINKMKEEETIDFDNDWKVITLFIGGNDLCNWCNSQGVERPTRTPEAFTGYIEEALQILYDGMPRAFVNVVSVLQVHQVSKMTGRICDAFHYAWCEDGMVFPEETLAQTRRYHVAVRDMVNSGKFDDREDFAVVIQPFLEETQLPELEGGEADLSYFAPDCFHFSSKAHAVAGKELWNNMNQKEGEKSNQWDARSDELLCPTEEFPYLYTRKNSEDDFDITPPGDGSSGVTAVRLTTVLLAIVSFVLSTYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HD84</accession>
    <name>A0A9Q1HD84_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Zinc finger protein ZFPM1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HOLleu_12802</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Nanhai2018</strain>
            <tissue evidence="16">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HD84"/>
    <dbReference type="OrthoDB" id="8742770at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061629">
        <property type="term" value="F:RNA polymerase II-specific DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059121">
        <property type="entry name" value="CCHC_ZFPM2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039746">
        <property type="entry name" value="FOG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034731">
        <property type="entry name" value="Znf_CCHC_FOG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12958">
        <property type="entry name" value="FRIEND OF GATA2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12958:SF3">
        <property type="entry name" value="ZINC FINGER PROTEIN USH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25445">
        <property type="entry name" value="CCHC_ZFPM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51810">
        <property type="entry name" value="ZF_CCHC_FOG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0010">Activator</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="171"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC FOG-type" evidence="15">
        <location>
            <begin position="372"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="378"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="716"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC FOG-type" evidence="15">
        <location>
            <begin position="981"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="64"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="140"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="246"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="282"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="405"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="512"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="617"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="772"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="815"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="934"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="145"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="295"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="405"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="428"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="513"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="524"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="617"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="666"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="780"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="790"/>
            <end position="800"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ8041879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1022" mass="114278" checksum="D32A60F648773339" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HEI0</accession>
    <name>A0A9Q1HEI0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Nori-2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">TP53-regulating kinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">p53-related protein kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">HOLleu_13196</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">Nanhai2018</strain>
            <tissue evidence="21">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. TP53RK has ATPase activity in the context of the EKC/KEOPS complex and likely plays a supporting role to the catalytic subunit OSGEP. Atypical protein kinase that phosphorylates 'Ser-15' of p53/TP53 protein and may therefore participate in its activation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="16">Component of the EKC/KEOPS complex composed of at least GON7, TP53RK, TPRKB, OSGEP and LAGE3; the whole complex dimerizes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. BUD32 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8042196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HEI0"/>
    <dbReference type="OrthoDB" id="3399at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000408">
        <property type="term" value="C:EKC/KEOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070525">
        <property type="term" value="P:tRNA threonylcarbamoyladenosine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000201">
        <property type="entry name" value="TP53-regulating kinase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000323">
        <property type="entry name" value="TP53-regulating kinase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022495">
        <property type="entry name" value="Bud32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03724">
        <property type="entry name" value="arch_bud32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12209:SF0">
        <property type="entry name" value="EKC_KEOPS COMPLEX SUBUNIT TP53RK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12209">
        <property type="entry name" value="NON-SPECIFIC SERINE/THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06293">
        <property type="entry name" value="Kdo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Protein kinase" evidence="20">
        <location>
            <begin position="8"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00056624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00062157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00079584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00080585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00081359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAJ8042196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="230" mass="26345" checksum="51E1CE732A3A4F28" modified="2023-09-13" version="1">MATKPSDLSEVDLIQQGAEARVYSGHFAGEPCIIKERFKKTYRHPTLEERLTQRRIQQEAKALLRCRKAGIAVPSVYFIDSTNNCIYLEKIEPSLTVREFIQQLPLPYSDEDNNKLYAIALKIGSVLASLHNTDTIHGDLTTSNMLLKFPHDAFELYLIDFGLSQVSQLHEDKGVDLYVLEKAFLSTHPKTEHVFEEILKSYQKNNKKAVEVLKKLDEVRLRGRKRVMVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HFL4</accession>
    <name>A0A9Q1HFL4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein ARV</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_07395</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Mediator of sterol homeostasis involved in sterol uptake, trafficking and distribution into membranes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Endoplasmic reticulum membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the ARV1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HFL4"/>
    <dbReference type="OrthoDB" id="2192830at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032366">
        <property type="term" value="P:intracellular sterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097036">
        <property type="term" value="P:regulation of plasma membrane sterol distribution"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006665">
        <property type="term" value="P:sphingolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016125">
        <property type="term" value="P:sterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007290">
        <property type="entry name" value="Arv1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14467">
        <property type="entry name" value="ARV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14467:SF0">
        <property type="entry name" value="PROTEIN ARV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04161">
        <property type="entry name" value="Arv1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7 10" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="112"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="157"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU368065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8044609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="241" mass="27432" checksum="3C83C0547EC260F7" modified="2023-09-13" version="1">MEKRKVVCIECGEAAPRLLKIYNSDIIKLSYCDHCNKLVDKYVEFDPVIILLDVLLFKPQAYRHILFNMQIQFQWKLCVISLLCGAYLKWAFNHHKSSENTKDGFAGSFLHYALQIEFYIVFLLAAIDLAAYLGGIHLGCKIYARTTEKPVNERLPILHNAIVLSQAGKLLVIPAVIWGQSHILTSFWLTELFVFTFVSQSLRVTLNCSRSTVALLLLAGYLTEISVSQSAQILQEFLTDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HGB7</accession>
    <name>A0A9Q1HGB7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_11296</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HGB7"/>
    <dbReference type="OrthoDB" id="10063766at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8043966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="110" mass="13056" checksum="B3D38652FE7AB11F" modified="2023-09-13" version="1">MPPDNAFKHLGLTTLELRRIRGDMLQVYKFLFERNPLSSCNYLKVQCNSRVRGHCKKLVKNFARLDIRKFSFSHRVVNEWNSLPEWVVNSTSVHCFKVNIDKFFHKCGRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HGL3</accession>
    <name>A0A9Q1HGL3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Mitochondrial import inner membrane translocase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_08037</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Heterohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Mitochondrion inner membrane</location>
            <topology evidence="8">Peripheral membrane protein</topology>
            <orientation evidence="8">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="8">The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the small Tim family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HGL3"/>
    <dbReference type="OrthoDB" id="1551503at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.810">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit tim13 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050673">
        <property type="entry name" value="Mito_inner_translocase_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004217">
        <property type="entry name" value="Tim10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035427">
        <property type="entry name" value="Tim10-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13172">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM9B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02953">
        <property type="entry name" value="zf-Tim10_DDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144122">
        <property type="entry name" value="Tim10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0143">Chaperone</keyword>
    <keyword evidence="7 8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="4 8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0811">Translocation</keyword>
    <keyword evidence="1 8" id="KW-0813">Transport</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Tim10-like" evidence="9">
        <location>
            <begin position="11"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU367043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8045105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="89" mass="10399" checksum="614A346309F1ABFC" modified="2023-09-13" version="1">MASQQMGQDLEVKQLKEFLGSYNRMTELCFTDCVDDFTTRKASEAELKCTHRCLEKFLKVSQRITTRFQEYQVQQNEGLMAAQAKQIPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HGT0</accession>
    <name>A0A9Q1HGT0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_07199</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HGT0"/>
    <dbReference type="OrthoDB" id="8903066at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8044446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="191" mass="21446" checksum="C6E8694901D39DB4" modified="2023-09-13" version="1">MVEVVGEPAPHTFEDMTTRMAIPDVRFHGSYPDRLDERIAYGNLPRWIVQGVDVPGVNIPQANYAKQLNQLPPLYAGHSTGVIKHSYGARPDPKVKAYTIQMPIPAAGANGYTSYVSKKSPRISLSDSPYVYSASHLGTQPKRSWKFNMYNQHAKQGFKFWLEEKKPFSQSQKYTFGSTKTFHGLGNAPRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HHG0</accession>
    <name>A0A9Q1HHG0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">SNW domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_07966</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SNW family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HHG0"/>
    <dbReference type="OrthoDB" id="666364at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017862">
        <property type="entry name" value="SKI-int_prot_SKIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004015">
        <property type="entry name" value="SKI-int_prot_SKIP_SNW-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12096">
        <property type="entry name" value="NUCLEAR PROTEIN SKIP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02731">
        <property type="entry name" value="SKIP_SNW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SKI-interacting protein SKIP SNW" evidence="4">
        <location>
            <begin position="172"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="203"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="327"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="468"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="295"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="343"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="368"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="468"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8045043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="537" mass="60914" checksum="D569C6779E92B140" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HIB5</accession>
    <name>A0A9Q1HIB5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ATR-interacting protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_05535</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HIB5"/>
    <dbReference type="OrthoDB" id="6428926at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000077">
        <property type="term" value="P:DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033349">
        <property type="entry name" value="ATRIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28594">
        <property type="entry name" value="ATR-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28594:SF1">
        <property type="entry name" value="ATR-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="94"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="179"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="338"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="491"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="663"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="228"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="59"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="94"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="179"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="370"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="413"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="499"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="676"/>
            <end position="689"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8046755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="742" mass="82355" checksum="8D9C7603ED7C7960" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HLI8</accession>
    <name>A0A9Q1HLI8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP-dependent DNA helicase</fullName>
            <ecNumber evidence="1">5.6.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_03795</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.3" evidence="1"/>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HLI8"/>
    <dbReference type="OrthoDB" id="416437at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010285">
        <property type="entry name" value="DNA_helicase_pif1-like_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051055">
        <property type="entry name" value="PIF1_helicase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47642">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47642:SF5">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05970">
        <property type="entry name" value="PIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0227">DNA damage</keyword>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="1" id="KW-0234">DNA repair</keyword>
    <keyword evidence="1 3" id="KW-0347">Helicase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA helicase Pif1-like DEAD-box helicase" evidence="2">
        <location>
            <begin position="56"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8050550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="392" mass="45023" checksum="346B42F55D0195D4" modified="2023-09-13" version="1">MVEDNFESEILSEPNCIPDFAPAQSSVSNTFVNLPKLHNVEYSTNTDTDNTSYQSLNDQQRKLFRYISSWCYKKTQDENTPPFYIFLSGGAGTGKSLLTKCIKTEVEKIFQTTSPSPDCLSVLVLAFTGTAAFNIHGQTIHSALSIRNFRMPYIPLTQDTLNTLTIKYERLISSIIDEISMVNVEMLSYISCRLQQIKGRSSQPFGNVFILAVGDFYRLPPIIGKPLFDIDPGALVNLWSLFKKWELQQIMRQKNDHQFAQLLNRIRTLQKGHNIQESDQKMLLDRLTHKVLNYPQDAMHIYSRNSDVDTHNTYMLNRYCHNIKVIHALDVYYDKQGQQRKLKQPLQFAKHCNLPVKIEIAVGARIMLTKNLDVSDGLVAFYVRRNGHEETK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0Y9Z7</accession>
    <name>A0A9Q0Y9Z7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_42813</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000139">
        <property type="protein sequence ID" value="KAJ8018922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0Y9Z7"/>
    <dbReference type="OrthoDB" id="10068277at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8018922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="116" mass="13140" checksum="51BC1B713F023508" modified="2023-09-13" version="1">MAPALRRLKNHAQEIQFLSKCSPRQRKAFLKHADPKLVISLCECASNIVKGIVPLYRTQKSKLSRYKKHLRDLSNKRLSNKKRKDILVQRGGILSLILKPIIQSLGGMLLGAVMPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YAZ6</accession>
    <name>A0A9Q0YAZ6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HECT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_43999</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000576">
        <property type="protein sequence ID" value="KAJ8018161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YAZ6"/>
    <dbReference type="OrthoDB" id="10038899at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8018161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="638" mass="72971" checksum="AA77318239405448" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YBH0</accession>
    <name>A0A9Q0YBH0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_43542</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000349">
        <property type="protein sequence ID" value="KAJ8018459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YBH0"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8018459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="696" mass="80530" checksum="8BB23617F99E7095" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YBH5</accession>
    <name>A0A9Q0YBH5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_43520</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000342">
        <property type="protein sequence ID" value="KAJ8018476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YBH5"/>
    <dbReference type="OrthoDB" id="10066957at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8018476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="70" mass="7990" checksum="BD3226D8116C77AA" modified="2023-09-13" version="1">MQARRKLKATDIVIKEDLTHANYTLLSQTTKHDKVVNCWTSDGHLRALVKTNDQREIKVTINSVSNLSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YES3</accession>
    <name>A0A9Q0YES3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Carbohydrate sulfotransferase</fullName>
            <ecNumber evidence="9">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_40105</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Golgi apparatus membrane</location>
            <topology evidence="1 9">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the sulfotransferase 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="9"/>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YES3"/>
    <dbReference type="OrthoDB" id="2019940at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016051">
        <property type="term" value="P:carbohydrate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018011">
        <property type="entry name" value="Carb_sulfotrans_8-10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005331">
        <property type="entry name" value="Sulfotransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12137">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12137:SF54">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03567">
        <property type="entry name" value="Sulfotransfer_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="8 9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8020499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="288" mass="34202" checksum="D47BFAC247899795" modified="2023-09-13" version="1">MHSPWKNITDTQAFVQYKRHRLLAEACKKYPELSEGKLSVDTQRHILVNEKQKLLYCFVPKVGCSNWKRVMMKLAGTTSKKLSRITSQDAHAKNGLRNLLNYEQNERRTILATYTAFMYARDPFARIVSAYQNKFGDVSVYRKETLIHKTAKMMIKKYRRDPSPKELETGENVTWTEFVNFLTDPVKNYFNEHWREMYKLCSPCHVNYDFLGHLETLDDDVQYMFQELHLEDKIKYPSSKTSHPTNSTNTYLEFYSQLTTEQIQRLWKIYEKDFDLFGYKKPNFVSST</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YF46</accession>
    <name>A0A9Q0YF46_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_42802</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000137">
        <property type="protein sequence ID" value="KAJ8018927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YF46"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8018927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="133" mass="15071" checksum="4BBF243D49871FA6" modified="2023-09-13" version="1">MISISEVWLNNCYSTVDLRGGNHLQLKSNLSIPVLTTLNNLSIWEEGREITSEEFTGILQYSSKCQALRELMFWSCLLPPSRQAESVSVLRSRNVKVTDVLLYTVVYNHNSSSGGLFLRTFFSFSDELAVSRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YGW7</accession>
    <name>A0A9Q0YGW7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12 13">U6 snRNA-associated Sm-like protein LSm5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LSM5</name>
        <name evidence="15" type="ORF">HOLleu_41808</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Nanhai2018</strain>
            <tissue evidence="15">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Plays a role in U6 snRNP assembly and function. Binds to the 3' end of U6 snRNA.</text>
    </comment>
    <comment type="function">
        <text evidence="10">Plays a role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex). The heptameric LSM2-8 complex binds specifically to the 3'-terminal U-tract of U6 snRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the precatalytic spliceosome (spliceosome B complex). Component of the U4/U6-U5 tri-snRNP complex, a building block of the precatalytic spliceosome (spliceosome B complex). The U4/U6-U5 tri-snRNP complex is composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, SNRNP40, SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, DDX23, CD2BP2, PPIH, SNU13, EFTUD2, SART1 and USP39, plus LSM2, LSM3, LSM4, LSM5, LSM6, LSM7 and LSM8. LSM2, LSM3, LSM4, LSM5, LSM6, LSM7 and LSM8 form a heptameric, ring-shaped subcomplex (the LSM2-8 complex) that is part of the U4/U6-U5 tri-snRNP complex and the precatalytic spliceosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">LSm subunits form a heteromer with a doughnut shape.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8019992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YGW7"/>
    <dbReference type="OrthoDB" id="429711at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990726">
        <property type="term" value="C:Lsm1-7-Pat1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005688">
        <property type="term" value="C:U6 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01732">
        <property type="entry name" value="LSm5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000003">
        <property type="entry name" value="U6 snRNA-associated Sm-like protein LSm5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033871">
        <property type="entry name" value="LSm5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20971">
        <property type="entry name" value="U6 SNRNA-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20971:SF0">
        <property type="entry name" value="U6 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3 13" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7 13" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 13" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 13" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 13" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Sm" evidence="14">
        <location>
            <begin position="12"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00056431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00063389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00067759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU365055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ8019992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="90" mass="9821" checksum="0B182E579C6ED069" modified="2023-09-13" version="1">MAAPASNPSQLLPLELVDKCIGSRIHIVMKSDKEIVGTLLGFDDFVNMVLEDVIEFEITAEGRRVTKLDQILLNGNNITMLIPGSEGPEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YHK9</accession>
    <name>A0A9Q0YHK9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Helitron helicase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_42926</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000165">
        <property type="protein sequence ID" value="KAJ8018856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YHK9"/>
    <dbReference type="OrthoDB" id="10063525at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046700">
        <property type="entry name" value="DUF6570"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025476">
        <property type="entry name" value="Helitron_helicase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20209">
        <property type="entry name" value="DUF6570"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14214">
        <property type="entry name" value="Helitron_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6570" evidence="3">
        <location>
            <begin position="11"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="Helitron helicase-like" evidence="2">
        <location>
            <begin position="195"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="112"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="114"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF20209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8018856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="405" mass="47249" checksum="6047BB242F6187D4" modified="2023-09-13" version="1">MQIREFPRGRKLGLKGNANVPADVSTTERPLPRSIDETHTIPVKFKRKLSFKHSVQSHNVRPNKVVNAALWLVNNSKLYRDEGVTIDKSWRENLSNMKQDWKEFLDPVERDSYTEGSLNTNEDSLSVDQNQSEMTPDSECFAPGEGIVPLTFFKTNILKNSSFLTFYQQPRHKSKVRVPNSSISKWELRHKDRRFAKSVLNIFYKAKKLQINQIQQKATLSLRKKKIGSKKFTAEVFKSMERVKELLCLDEGFRVLRTVRGSPPYWENTKKELFAMIRQLGIPTWFMSFSAAETRWLHFLRILGKTVHSKEYSDAELLNLTWHEKSELIQSDPVTCARHFDYMIRRFINDVLLSSYHPVGEIIDHFYRVEFQQRGSPHIHMLAWVNNAPVYANASNNEIALFIDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YIW8</accession>
    <name>A0A9Q0YIW8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_38588</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YIW8"/>
    <dbReference type="OrthoDB" id="10491205at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040451136" description="Ig-like domain-containing protein" evidence="3">
        <location>
            <begin position="22"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="270"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="25"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="327"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8021404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="409" mass="44934" checksum="CD482C94FF13138C" modified="2023-09-13" version="1">MEFNINLLIILCSTIFHTISGNFGDDIIEIQPMNPKVREGDNIILTCFSPIPGALVSWLQVSQTSTVFESVERYSFPGGKVIRLGNASRFPTEESSSVAFLCSGNVNGVEEQQLSTVTVVARDFSMAVTEQSSLVIFPLIPVARNGTNFTLTCLAPSENTSDIGWYIPGEQSAKFETFEIRKFDNGANITLIGINVDSTETVIRIVCLQTDETFISEQVDIRVTETIPTTELPISSKSGKERSTIITNPPILVTTPYVTASYVGGIPLSLVLIAIGIGAGLAVFVICLILLSVYVLVVFRSQGWGPFKSSEDVDDVFKANPREIDNYRPYQSLGDKDDASNPRGVGNKEGYADLRQYEEPRLYMRLESKSSSKPQKQEYNVPEAIYDGGDTTAVQMISFRHTDSKEGDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YKH7</accession>
    <name>A0A9Q0YKH7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Insulin-like growth factor-binding protein complex acid labile subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_38123</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8023051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YKH7"/>
    <dbReference type="OrthoDB" id="6159398at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366">
        <property type="entry name" value="IG(IMMUNOGLOBULIN) AND LRR(LEUCINE RICH REPEAT) DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366:SF96">
        <property type="entry name" value="LEUCINE RICH REPEAT CONTAINING 53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040252267" evidence="6">
        <location>
            <begin position="25"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="457"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="337"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8023051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="644" mass="72745" checksum="0F06C79424A001D9" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YKS9</accession>
    <name>A0A9Q0YKS9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">tRNA pseudouridine synthase</fullName>
            <ecNumber evidence="6">5.4.99.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_39388</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>uridine(38/39/40) in tRNA = pseudouridine(38/39/40) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:22376"/>
            <dbReference type="Rhea" id="RHEA-COMP:10085"/>
            <dbReference type="Rhea" id="RHEA-COMP:10087"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="EC" id="5.4.99.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the tRNA pseudouridine synthase TruA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.12" evidence="6"/>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8022017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YKS9"/>
    <dbReference type="OrthoDB" id="271910at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160147">
        <property type="term" value="F:tRNA pseudouridine(38-40) synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02570">
        <property type="entry name" value="PseudoU_synth_EcTruA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.660">
        <property type="entry name" value="Pseudouridine synthase I, catalytic domain, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.580">
        <property type="entry name" value="Pseudouridine synthase I, catalytic domain, N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00171">
        <property type="entry name" value="TruA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001406">
        <property type="entry name" value="PsdUridine_synth_TruA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020097">
        <property type="entry name" value="PsdUridine_synth_TruA_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020095">
        <property type="entry name" value="PsdUridine_synth_TruA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020094">
        <property type="entry name" value="TruA/RsuA/RluB/E/F_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142:SF0">
        <property type="entry name" value="TRNA PSEUDOURIDINE SYNTHASE-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01416">
        <property type="entry name" value="PseudoU_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001430">
        <property type="entry name" value="tRNA_psdUrid_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Pseudouridine synthase I TruA alpha/beta" evidence="7">
        <location>
            <begin position="159"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="53"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR001430-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001430-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8022017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="302" mass="35177" checksum="69D534DCC0B7D80C" modified="2023-09-13" version="1">MLLERCLHHFFSGLAKASQKWSEKQGVLDVLEEAIQRLTPIQVENVTSSCRTDSGVHALVNTIHVDLERPSHKQPFTPHSLVRGINRELVGEDVRCVQWVIKACKVQDTFHARHNVVRRDYLYRVVLGCEYPKEMPLFEENRSYAIRESRLDIQKMEEASRLFIGLHDFASFRNLRHDFKDQYTKREMYSVSIYHPCSHHDNYLLHPFSCRYLYLEFSARSYLYRQIRRMVGVLLAVGRGRLTPSDVQDILQSRDNANLRGTKPAPAGGLYLKDVIYDEKDLQYNSNEKDVEEGGDDSKCVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YLY7</accession>
    <name>A0A9Q0YLY7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Coenzyme Q-binding protein COQ10-like B, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_34857</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Required for the function of coenzyme Q in the respiratory chain. May serve as a chaperone or may be involved in the transport of Q6 from its site of synthesis to the catalytic sites of the respiratory complexes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Interacts with coenzyme Q.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the COQ10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YLY7"/>
    <dbReference type="OrthoDB" id="292693at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048039">
        <property type="term" value="F:ubiquinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045333">
        <property type="term" value="P:cellular respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07813">
        <property type="entry name" value="COQ10p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.530.20:FF:000002">
        <property type="entry name" value="Coenzyme Q-binding protein COQ10 homolog, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044996">
        <property type="entry name" value="COQ10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005031">
        <property type="entry name" value="COQ10_START"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12901:SF10">
        <property type="entry name" value="COENZYME Q-BINDING PROTEIN COQ10, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12901">
        <property type="entry name" value="SPERM PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03364">
        <property type="entry name" value="Polyketide_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Coenzyme Q-binding protein COQ10 START" evidence="10">
        <location>
            <begin position="82"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8024834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="236" mass="27308" checksum="4511592A1E61758B" modified="2023-09-13" version="1">MNNTNYRVATVLTRSLRYVLSETKIIRVNRPFCMMCVRAAHHQALMRVNYTKTPLVLIPHRDLFSIPSPLNKTKEYAERRIIGYSMQDMYEVVANVEDYKEFVPWCTQSKIVQRKAGHFKAQLEIGFNPLVERYMSTVTLVKPHLVKAVCTDGRLFNHLITIWRFSPGIRGKPDTCTIDFAISFEFRSVLHSNLSALFFDEVVKKMVKAFEKRAEKLYGPQAAKKPAPDYSIVKDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YMY9</accession>
    <name>A0A9Q0YMY9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_33034</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YMY9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8025468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="51" mass="5546" checksum="7622690594DFCB16" modified="2023-09-13" version="1">MANLLNDYFVSVFTKESSVLGTCRDSDNSSLVSVDVVFSEELVCNKDSIAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YS95</accession>
    <name>A0A9Q0YS95_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_31496</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YS95"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8026610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="81" mass="9035" checksum="10BB8E9A7027A859" modified="2023-09-13" version="1">MLYGEKMNPIIFGGSQKSSGVTGGQTLKSMLTRYLQMRFGKKMTPIIYGGGQRSSEVTGGQTLKTLLTRYLQVRSLDRFHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BCQ4</accession>
    <name>A0A9Q1BCQ4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RBR-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="2">2.3.2.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_40308</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.31"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.31" evidence="2"/>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BCQ4"/>
    <dbReference type="OrthoDB" id="1431934at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20338">
        <property type="entry name" value="BRcat_RBR_RNF19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20355">
        <property type="entry name" value="Rcat_RBR_RNF19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16775">
        <property type="entry name" value="RING-HC_RBR_RNF19A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1750:FF:000001">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000052">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031127">
        <property type="entry name" value="E3_UB_ligase_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11685">
        <property type="entry name" value="RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01485">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22191">
        <property type="entry name" value="IBR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00647">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="362"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="415"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="13">
        <location>
            <begin position="135"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="12">
        <location>
            <begin position="139"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="17"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="636"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="792"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="37"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="56"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="93"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="109"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="655"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="672"/>
            <end position="697"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ8020658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="828" mass="88870" checksum="67D125A22BEB0791" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BCR9</accession>
    <name>A0A9Q1BCR9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Neuronal acetylcholine receptor subunit alpha-9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_39136</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the ligand-gated ion channel (TC 1.A.9) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BCR9"/>
    <dbReference type="OrthoDB" id="5975154at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005230">
        <property type="term" value="F:extracellular ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18997">
        <property type="entry name" value="LGIC_ECD_nAChR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19051">
        <property type="entry name" value="LGIC_TM_cation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000028">
        <property type="entry name" value="AcetylCholine Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000073">
        <property type="entry name" value="Neuronal acetylcholine receptor subunit alpha-9-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="138"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="166"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="199"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="242"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="383"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="6">
        <location>
            <begin position="1"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="7">
        <location>
            <begin position="143"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8021830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="414" mass="47506" checksum="33604389C64A99CD" modified="2023-09-13" version="1">MRIPATEIWVPDVTLYDNADSNDYVAHSRSSNAIVSHKGNIDLWSKPTLVKSTCKIDVKYFPFDMQECSMKFGSWTYTSFQMNLTKHAPNPELSELVPNEQWDLEYAVTRRHSVKYECCPERYLDVTFYFGLKRKPLYYIYNLLMPCMLLSSLSLLGFFMPYDVGVVKVSLSITLILSLTVFLLLVAEMMPRTSEEVPLIGQYYAATMFMISISTAMNVFVLNINEKGGLVHAREVPRWLRVIALEYLAVVFWVTPCPCVRMKKKSRTQHEHHELRYKPVNNQNTHIVTFDTTSPRNGGVTSNHVVGNRGSRYMNGPNPPDFDSIVREQNGSESETISERRLARMDKVVSEILKHLKALDRKKEKATQLKKDWALVAKVLDRLLLMIFFVSTVVTTICLLSQRAVDKTPPPEDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BDD8</accession>
    <name>A0A9Q1BDD8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_36065</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BDD8"/>
    <dbReference type="OrthoDB" id="6089094at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="382"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="652"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="467"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="383"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="667"/>
            <end position="678"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8023586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="777" mass="88844" checksum="ABB6FF0AC6B25654" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BEA0</accession>
    <name>A0A9Q1BEA0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Band 4.1-like protein 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_34536</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BEA0"/>
    <dbReference type="OrthoDB" id="6235974at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031032">
        <property type="term" value="P:actomyosin structure organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000003">
        <property type="entry name" value="Tyrosine-protein phosphatase non-receptor type 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018979">
        <property type="entry name" value="FERM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23280">
        <property type="entry name" value="4.1 G PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23280:SF25">
        <property type="entry name" value="MOESIN_EZRIN_RADIXIN HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00373">
        <property type="entry name" value="FERM_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09379">
        <property type="entry name" value="FERM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FERM" evidence="1">
        <location>
            <begin position="1"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8024591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="244" mass="28359" checksum="A4ECC04B26A4E3B0" modified="2023-09-13" version="1">MRVVIEVEILKKEKAHDLLEQVYYTLDIIEKDYFGLSYIDADRVQHWLDPTKTLKKQMKPWKYYLGGSEYYLGGSEYYLGGSEYYLGGSEYYLGGSEYYLGGSEYYLGGSEYYLGGSEYYLGGSEYYLGGSEYYLGGSEYYLGGKPPPYKFTFRVKFYSSEPNNLHEEITRYIFFLQLKKDILDGRLVPPYDTSVELAALSLQSELGDYDPEEHTPELVSEFRFTPDQTEAMEVDIVEKFKEMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BGH3</accession>
    <name>A0A9Q1BGH3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">(S)-2-hydroxy-acid oxidase</fullName>
            <ecNumber evidence="2">1.1.3.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_31754</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>2-hydroxyoctanoate + O2 = 2-oxooctanoate + H2O2</text>
            <dbReference type="Rhea" id="RHEA:67940"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:133514"/>
            <dbReference type="ChEBI" id="CHEBI:176689"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:67941"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a (2S)-2-hydroxycarboxylate + O2 = a 2-oxocarboxylate + H2O2</text>
            <dbReference type="Rhea" id="RHEA:16789"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="ChEBI" id="CHEBI:58123"/>
            <dbReference type="EC" id="1.1.3.15"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:16790"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.3.15" evidence="2"/>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BGH3"/>
    <dbReference type="OrthoDB" id="25826at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003973">
        <property type="term" value="F:(S)-2-hydroxy-acid oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001561">
        <property type="term" value="P:fatty acid alpha-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02809">
        <property type="entry name" value="alpha_hydroxyacid_oxid_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000056">
        <property type="entry name" value="hydroxyacid oxidase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012133">
        <property type="entry name" value="Alpha-hydoxy_acid_DH_FMN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000262">
        <property type="entry name" value="FMN-dep_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037396">
        <property type="entry name" value="FMN_HAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008259">
        <property type="entry name" value="FMN_hydac_DH_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578:SF149">
        <property type="entry name" value="2-HYDROXYACID OXIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578">
        <property type="entry name" value="S -2-HYDROXY-ACID OXIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01070">
        <property type="entry name" value="FMN_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000138">
        <property type="entry name" value="Al-hdrx_acd_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00557">
        <property type="entry name" value="FMN_HYDROXY_ACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51349">
        <property type="entry name" value="FMN_HYDROXY_ACID_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0288">FMN</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FMN hydroxy acid dehydrogenase" evidence="9">
        <location>
            <begin position="1"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="274"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="75"/>
            <end position="77"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="250"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="272"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="274"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="277"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="306"/>
            <end position="310"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="329"/>
            <end position="330"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000138-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000138-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8026811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="385" mass="42313" checksum="E712CED3C7589A09" modified="2023-09-13" version="1">MRSIDDVEEEAKKSLPEWMMSYYATGTGDEQTLEENKAAYKRFLFKPRIMCADKATDISTSVLGRRVRIPIGVSPTALQGGAHPRAEMATAEGAERCGTVMIMSSWSQVRIEDVAKSAPNGNLWMQTYLNKDRRNTQNIVERAEKAGFNAIVVTVDSPCVGIRKKKGYQGGDQFIKTLKGINVRDTWQELFLLSLARFVNYEGAVDDIAKARASGDVKLFKYVWDQSLSYPKWEYVEWLKTVTTLPLIMKGIMTVENAREAVAVGAKAVIVSNHGGRQLDGLPSTIEVLPEIVDALRGSGVEVYVDGGIRNGSDVLKALALGAQFVFCGRPAVWGLGTNGADGVAEVLQILENELKVTMVLCGCTKLTDIDRSLVIHESQLKCKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BGN1</accession>
    <name>A0A9Q1BGN1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA-directed DNA polymerase</fullName>
            <ecNumber evidence="2">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_31989</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="2"/>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BGN1"/>
    <dbReference type="OrthoDB" id="5989329at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.690">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1600.10">
        <property type="entry name" value="Palm domain of DNA polymerase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004868">
        <property type="entry name" value="DNA-dir_DNA_pol_B_mt/vir"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023211">
        <property type="entry name" value="DNA_pol_palm_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33568">
        <property type="entry name" value="DNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33568:SF3">
        <property type="entry name" value="DNA-DIRECTED DNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03175">
        <property type="entry name" value="DNA_pol_B_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0235">DNA replication</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA-directed DNA polymerase family B mitochondria/virus" evidence="9">
        <location>
            <begin position="44"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed DNA polymerase family B mitochondria/virus" evidence="9">
        <location>
            <begin position="143"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8026991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="321" mass="37115" checksum="920D953DCF0BE950" modified="2023-09-13" version="1">MSMDGYQYVEIWEHEWQDLKKTLSSEMKAEIAKFPRNLDPLVPREAFFGGRTNGIKLYYEVKKGEQIKYLDFCSLYPFTNKYCSYPIGHPKILTRDLSHDITKYYGLVRFKQEASGWPDWCVTEVDRQRYLTENAANEGIKLEPSKIEKNPGLRALAKLMLNSFWGKFGQHEDLVKTEIVSEPGRLYDLMKACDETEVKDVRVINDGILEVQFKDKQNFTYTNSRVNVVIAAFTTCHARLRLYDVIDRLSERVLYFDKDSVMYISRPGDWDPPTGDYLGDLTNEISPKDGNFIRSFVTGCPKNYAYTLDTGKSVCTVRGIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BH64</accession>
    <name>A0A9Q1BH64_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_35128</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8025039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BH64"/>
    <dbReference type="OrthoDB" id="248923at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13986">
        <property type="entry name" value="STKc_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052239">
        <property type="entry name" value="Ser/Thr-specific_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45998">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45998:SF2">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="17"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8025039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="302" mass="34249" checksum="9776909CD3B9054A" modified="2023-09-13" version="1">MGASCTRERLTIDGRRFYTNKRLGEGGFSFVDQVEESSTGKKYALKRIQCHSTAEEKAAEEEAKFHQLFDSPYLISVEAWSKKKLSGDDSEVLLVLPYFKRGTLQDELDRLSARGDHIPEDRLWKLIRGICEGVHALHHADPPVAHRDLKPGNVMLAEDDTPVIMDFGSMKQGPVEVKGRSQAVALQDIAAEKCSMLFRAPELFHVDSHCIVDEKVDVWSLGCLIFNLAFLQSPFEPVYQRGDSLHLAVLGRNYKFPENSRYSPGLKDLVDSMLTVETIERPNMDWVLDKLSKIDQDRQNRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BH81</accession>
    <name>A0A9Q1BH81_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Fatty acid synthase</fullName>
            <ecNumber evidence="1">2.3.1.85</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HOLleu_31270</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Nanhai2018</strain>
            <tissue evidence="17">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>acetyl-CoA + n malonyl-CoA + 2n NADPH + 2n H(+) = a long-chain fatty acid + (n+1) CoA + n CO2 + 2n NADP(+).</text>
            <dbReference type="EC" id="2.3.1.85"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.85" evidence="1"/>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BH81"/>
    <dbReference type="OrthoDB" id="329835at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004312">
        <property type="term" value="F:fatty acid synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00833">
        <property type="entry name" value="PKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.366.10">
        <property type="entry name" value="Malonyl-Coenzyme A Acyl Carrier Protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001227">
        <property type="entry name" value="Ac_transferase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014043">
        <property type="entry name" value="Acyl_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018201">
        <property type="entry name" value="Ketoacyl_synth_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014031">
        <property type="entry name" value="Ketoacyl_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014030">
        <property type="entry name" value="Ketoacyl_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016036">
        <property type="entry name" value="Malonyl_transacylase_ACP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032821">
        <property type="entry name" value="PKS_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020841">
        <property type="entry name" value="PKS_Beta-ketoAc_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050091">
        <property type="entry name" value="PKS_NRPS_Biosynth_Enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43775">
        <property type="entry name" value="FATTY ACID SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43775:SF7">
        <property type="entry name" value="FATTY ACID SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00698">
        <property type="entry name" value="Acyl_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16197">
        <property type="entry name" value="KAsynt_C_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00109">
        <property type="entry name" value="ketoacyl-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02801">
        <property type="entry name" value="Ketoacyl-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00827">
        <property type="entry name" value="PKS_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00825">
        <property type="entry name" value="PKS_KS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55048">
        <property type="entry name" value="Probable ACP-binding domain of malonyl-CoA ACP transacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00606">
        <property type="entry name" value="KS3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52004">
        <property type="entry name" value="KS3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="13" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="11" id="KW-0520">NAD</keyword>
    <keyword evidence="9" id="KW-0521">NADP</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ketosynthase family 3 (KS3)" evidence="16">
        <location>
            <begin position="1"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00044883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS52004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ8026450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="919" mass="100816" checksum="A9C3A9EDC8229CD6" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BHJ6</accession>
    <name>A0A9Q1BHJ6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">(S)-2-hydroxy-acid oxidase</fullName>
            <ecNumber evidence="2">1.1.3.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_33121</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>2-hydroxyoctanoate + O2 = 2-oxooctanoate + H2O2</text>
            <dbReference type="Rhea" id="RHEA:67940"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:133514"/>
            <dbReference type="ChEBI" id="CHEBI:176689"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:67941"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a (2S)-2-hydroxycarboxylate + O2 = a 2-oxocarboxylate + H2O2</text>
            <dbReference type="Rhea" id="RHEA:16789"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="ChEBI" id="CHEBI:58123"/>
            <dbReference type="EC" id="1.1.3.15"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:16790"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.3.15" evidence="2"/>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BHJ6"/>
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        <property type="term" value="F:(S)-2-hydroxy-acid oxidase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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        <property type="entry name" value="S -2-HYDROXY-ACID OXIDASE-RELATED"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0285">Flavoprotein</keyword>
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    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="264"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="78"/>
            <end position="80"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>FMN</name>
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    <feature type="binding site" evidence="8">
        <location>
            <position position="129"/>
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        <ligand>
            <name>glyoxylate</name>
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        </ligand>
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    <feature type="binding site" evidence="8">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="262"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
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        <location>
            <position position="267"/>
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        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
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        <location>
            <begin position="298"/>
            <end position="302"/>
        </location>
        <ligand>
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            <begin position="321"/>
            <end position="322"/>
        </location>
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            <name>FMN</name>
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            <dbReference type="ARBA" id="ARBA00001917"/>
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        <source>
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            <dbReference type="ARBA" id="ARBA00029327"/>
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        <source>
            <dbReference type="EMBL" id="KAJ8025534.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="379" mass="41430" checksum="66E8A9682BD00047" modified="2023-09-13" version="1">MTQPQTVRDYEELGLSKLWKFPTMYYMRGADDEQTARDSESAFQRYRLRPRILRGISSISKRCTVLGHEINFPICLAPTACCRFAHVEKEIGTCKAARKLRTLMVHSCASTTSIRELMEAVPGALVWQQLYLFKNKSLTQQMVKEAEASGVKAFVVTVDAAGGCNRRISPTTEGSTVLAGNIDFKLLNFEITTPDCAGFKQMGDQNLWAYCAYQCQESSNSIEDLRWIRSITTLPIIVKGIITGKDARRVIDEGIASAIIVSAHGGRQLDGIPAPIDALGEVLEAVRSTGSSTEVYMDGGVRSGSDVFKALAAGAKAVFIGRPALWALAANGSDGVAHLLEIFREELHDVMAQCGCATLEDITPSSVIHESKYLARLEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BJ27</accession>
    <name>A0A9Q1BJ27_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_32700</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
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    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
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        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 297-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon gypsy-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Integrase_cat-core"/>
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        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF13">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="2">
        <location>
            <begin position="753"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="222"/>
            <end position="275"/>
        </location>
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            <begin position="486"/>
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        <location>
            <begin position="586"/>
            <end position="626"/>
        </location>
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        <location>
            <begin position="222"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="237"/>
            <end position="259"/>
        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="261"/>
            <end position="270"/>
        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="486"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="530"/>
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        </location>
    </feature>
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        <location>
            <begin position="555"/>
            <end position="570"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="602"/>
            <end position="626"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8027533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="940" mass="106459" checksum="A34E388ADF1963D5" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BJB2</accession>
    <name>A0A9Q1BJB2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_29696</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8027682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BJB2"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="16"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8027682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="111" mass="13191" checksum="1F5A45BF8128A6C1" modified="2023-09-13" version="1">MKQRQVSHTTQHQYRVSSPIFFFFFFFFFFLSPLSRSRKKFSSNPSRASVFRQFYAKFLKPMRLDKSFLAIPRACQSRLATRNYGGKTARKSHLRRNSLLSPVDKRSPPNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BJR7</accession>
    <name>A0A9Q1BJR7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="21">Gamma-aminobutyric acid receptor subunit beta-3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">HOLleu_30030</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">Nanhai2018</strain>
            <tissue evidence="21">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="17">Postsynaptic cell membrane</location>
            <topology evidence="17">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the ligand-gated ion channel (TC 1.A.9) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8027933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BJR7"/>
    <dbReference type="OrthoDB" id="8890589at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005230">
        <property type="term" value="F:extracellular ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004890">
        <property type="term" value="F:GABA-A receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19049">
        <property type="entry name" value="LGIC_TM_anion"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000067">
        <property type="entry name" value="Glycine receptor subunit alpha-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000074">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006028">
        <property type="entry name" value="GABAA/Glycine_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002289">
        <property type="entry name" value="GABAAb_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00860">
        <property type="entry name" value="LIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01160">
        <property type="entry name" value="GABAARBETA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00253">
        <property type="entry name" value="GABAARECEPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0868">Chloride</keyword>
    <keyword evidence="11" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16 18" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 18" id="KW-0406">Ion transport</keyword>
    <keyword evidence="15" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="8 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="10 21" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0770">Synapse</keyword>
    <keyword evidence="3 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 18" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="157"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="187"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="222"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="359"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="19">
        <location>
            <begin position="1"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="20">
        <location>
            <begin position="164"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAJ8027933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="392" mass="45969" checksum="DB3D89E01DCBBDA8" modified="2023-09-13" version="1">MYLRQHWRDERLAYDSTYGNVSVDGRLADRIWVPDTYLVNDKRSLIHDVTVKNRLLRFHHDGTIMYGIRMTTVAACMMDLRNYPMDEQNCTLELESYGYTTDDITFTWQYGDESIEGFENIEMPQFALVEYDLNARNTVSAGSYPRLSMSFRIRRNIGFFILQTYLPSILIVILSWVSFWINHEATAARVALGITTVLTMTTISTSVRATLPRISYIKSIDIYVVTCFAFVFAALLEYAVVNFNHWAEKRKQMDNDSSRKNYHGYIPVSQNESPARRRILNSDDDDDEWSDGEVRNSSFRSYQYSQTNSPCRLRGRKNGKLRHAWLNLGRRHQRRKKKRRRPPLPNIKNVDSIDSMSRILFPVGFILFNVVYWFSYLKIFYGSEELPAMVQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BJW4</accession>
    <name>A0A9Q1BJW4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Epidermal growth factor-like protein 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_29898</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8027830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BJW4"/>
    <dbReference type="OrthoDB" id="10060424at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000038">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013032">
        <property type="entry name" value="EGF-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048530">
        <property type="entry name" value="FAM171_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024731">
        <property type="entry name" value="NELL2-like_EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052235">
        <property type="entry name" value="Nephronectin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24050">
        <property type="entry name" value="PA14 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24050:SF28">
        <property type="entry name" value="UROMODULIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12947">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10577">
        <property type="entry name" value="FAM171A1-2-B_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12661">
        <property type="entry name" value="hEGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1 6" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 9" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0768">Sushi</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040451739" evidence="9">
        <location>
            <begin position="26"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="10">
        <location>
            <begin position="120"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="10">
        <location>
            <begin position="198"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="10">
        <location>
            <begin position="243"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="11">
        <location>
            <begin position="777"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="36"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="540"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="36"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="805"/>
            <end position="832"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8027830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="853" mass="93279" checksum="F20FFBF4C912DD7D" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BME7</accession>
    <name>A0A9Q1BME7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SRCR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_28628</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BME7"/>
    <dbReference type="OrthoDB" id="536948at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000009">
        <property type="entry name" value="WC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48071:SF18">
        <property type="entry name" value="DELETED IN MALIGNANT BRAIN TUMORS 1 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48071">
        <property type="entry name" value="SRCR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00530">
        <property type="entry name" value="SRCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00258">
        <property type="entry name" value="SPERACTRCPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040413069" description="SRCR domain-containing protein" evidence="5">
        <location>
            <begin position="28"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="166"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="6">
        <location>
            <begin position="33"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="201"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="201"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="100"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8029273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="273" mass="30021" checksum="A8DA81D7CC692424" modified="2023-09-13" version="1">MATLKKEMSCIVCLLFSSYLLFGVSEAEKEWTIRLAGGKMACEGRVEVFRHGTWGTISNRDWDTKHAGVVCRQLGCGGEASVTIPSKFGGGDGPVWQVVCTGEERSIDACEASQMAPLDTSYSVASADFWDHDSDAGIRCHVETMDSLNPKGLTRNDYHTMLLTDAVIFAVLLVLLIILIVSGVFAYRRFSHKLTTTSKQSEVKVSHKELPKTPSKEDEKTGVPSLYCKELMVTSPSTSSKIEDKGTKDTEFELKQVRGGKVEEEEGVYITCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BMX7</accession>
    <name>A0A9Q1BMX7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Reverse transcriptase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_29069</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="414530at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.10.10:FF:000007">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 17.6-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000032">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 297-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon gypsy-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000020">
        <property type="entry name" value="Transposon Tf2-6 polyprotein-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF15">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50804">
        <property type="entry name" value="SCAN_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="3" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SCAN box" evidence="11">
        <location>
            <begin position="155"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="10">
        <location>
            <begin position="278"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase catalytic" evidence="13">
        <location>
            <begin position="678"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase" evidence="12">
        <location>
            <begin position="1089"/>
            <end position="1266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="25"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="239"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="25"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="52"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="250"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ8029627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1347" mass="153199" checksum="D7AE0C8639EFD460" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BN44</accession>
    <name>A0A9Q1BN44_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Fibronectin type-III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_29174</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BN44"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050991">
        <property type="entry name" value="ECM_Regulatory_Proteins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46708:SF2">
        <property type="entry name" value="FIBRONECTIN TYPE-III DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46708">
        <property type="entry name" value="TENASCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="2">
        <location>
            <begin position="47"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="2">
        <location>
            <begin position="155"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8029711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="370" mass="41157" checksum="B1F87E1C93D06C1E" modified="2023-09-13" version="1">MKKYKIKNNLYYVTSLQTFLEFTGHIYGEDWSSFFVPCDDTPDVYNPVRNLTAHPNGIGQLNLTWKPPDLDSTLFAAFSYVVTYVLHRHDACLQHVDLSGEISVNDTEVVLTGLEDFAIYSISVIPRTKSVRQNVNITVEGLPVQIVQKTLPSAPSRSPNIMVDGESTTQTSLTFTWDPVSCEDLNGVFIQYTYSFHQTDSDVIIAEGSIREQSLRRITFKGLTACTNYTFNIRVDNDAFNGKYNTKYATTSLSIPGNETNLILKPGTNDTCADGNTCLIATWQKPGSSSCPVDYQMISLHQIDVDNCRDTSPELISSSDFGSDQLEHVFSRLNPNSLYRATLSLGNSMGSVATTRELRAPEGGKLLIFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BNN6</accession>
    <name>A0A9Q1BNN6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_29386</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAM174 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BNN6"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009565">
        <property type="entry name" value="FAM174-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28607">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28607:SF4">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06679">
        <property type="entry name" value="DUF1180"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="113"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8029874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="174" mass="19110" checksum="F5497EE08ED3CD27" modified="2023-09-13" version="1">MSRNCIEFRVQTGTRDFGRLAHVTVQPSLFLMAFSQVLDKVLWVLPVVGEVLIANCFFLVSGEGNQVTARTTTVAAATIKVSSNGNSTSKQDGDGSDPYDAAKLLNQTNMNRMLVVLLTITVVTMCFFTCKYLTSKKAKKAKKYGVLATSGADVELRPLDDDEDDEEDVFNVNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BP63</accession>
    <name>A0A9Q1BP63_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_26504</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BP63"/>
    <dbReference type="OrthoDB" id="6091153at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45823">
        <property type="entry name" value="T-SNARE COILED-COIL HOMOLOGY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45823:SF1">
        <property type="entry name" value="T-SNARE COILED-COIL HOMOLOGY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="411"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="411"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8030173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="448" mass="50802" checksum="98F312473516FFF9" modified="2023-09-13" version="1">MERSSPVSTSAKSWVDILQRHLTDGDPDRQREGAVSSQAGGDIRVPSTTRQRLDSTEPLISFDSGGRQRSTQRTEYEVLSTTETDGPRRAAVLGSYEQILDGNPTWKGDDTREQPLQSSQRLGVKPELRNEIGGHPPPVNSWLDEMMKGRNYEYRGRSGMNTREQTNPFSKFLPIETKYPEKCEAPGNRGRPNVLPPPFDGTGNWDDYMVQFELIAELNAWDQIQKALYLAASLKGQARAILSDLDSTHRRDYSQLSAALSNRFSPANQTQLFRAMLKNRARKPDKSIPQLAQEIKRLTLQAYPEAPYETIETLARDYFIDSLGDTDTKSRVYQSRPRNLSDAVVVAVELESFFLAETKKGNLRRPIARAVVPPANDGDKVEKQMKFLGEALETLTSNFEKMQTQILKQETQLRDMRETKQKSTSPQLDEKRGNHRSWQGNERPLPKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BPW3</accession>
    <name>A0A9Q1BPW3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_26853</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BPW3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8030425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="66" mass="7892" checksum="16AF8DA1F4066910" modified="2023-09-13" version="1">MGKTSIIFYGFFSLSDVAKHFNYMLNELFKGYNIVQTNKDIYERKLQNWHCFGLHGLHIYKMVDLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BQD8</accession>
    <name>A0A9Q1BQD8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Y+L amino acid transporter 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_27294</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BQD8"/>
    <dbReference type="OrthoDB" id="3257095at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050598">
        <property type="entry name" value="AminoAcid_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785:SF375">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="73"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8030788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="136" mass="14855" checksum="5A85B061995DE193" modified="2023-09-13" version="1">MDGGWSTLNNLTEEIKKPEKTVPRAIFGTLIIVFCIYMSANVSYLTVMTPSEMLASEAVAVTFAIRTLNNWSWTVWLCVALSAAGQLTGALLSGTRMYFVASREGALPEVLSLVHVRRRTPLPAVAVTVSLVVMYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BRJ9</accession>
    <name>A0A9Q1BRJ9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Membrane magnesium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_27981</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins. May be involved in Mg(2+) transport.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the ER membrane protein complex (EMC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Golgi apparatus membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Early endosome membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the membrane magnesium transporter (TC 1.A.67) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8031296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BRJ9"/>
    <dbReference type="OrthoDB" id="44756at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072546">
        <property type="term" value="C:EMC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022890">
        <property type="term" value="F:inorganic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018937">
        <property type="entry name" value="MMgT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21181">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21181:SF7">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10270">
        <property type="entry name" value="MMgT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0967">Endosome</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU367002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8031296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="109" mass="12432" checksum="F8EB2AF357712F56" modified="2023-09-13" version="1">MPPPLYGTFLSATLWNSIPLHICQAENRSYIRLTEQEFNRLPADIVIECFVSLIVTCYGVINMAGKFKGIRAMADFQNKSMDTVGNRPSFYHFAHRGTKLYAGEPEEER</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BRX5</accession>
    <name>A0A9Q1BRX5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_24697</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BRX5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040286535" evidence="3">
        <location>
            <begin position="19"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="385"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="340"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8031491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="543" mass="60346" checksum="ECCF317E370B3C60" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BV07</accession>
    <name>A0A9Q1BV07_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">NLR family CARD domain-containing protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_23229</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BV07"/>
    <dbReference type="OrthoDB" id="427518at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004875">
        <property type="entry name" value="DDE_SF_endonuclease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006600">
        <property type="entry name" value="HTH_CenpB_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312">
        <property type="entry name" value="NACHT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312:SF2">
        <property type="entry name" value="NUCLEOTIDE-BINDING OLIGOMERIZATION DOMAIN-CONTAINING PROTEIN 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03184">
        <property type="entry name" value="DDE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51253">
        <property type="entry name" value="HTH_CENPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NACHT" evidence="2">
        <location>
            <begin position="198"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="HTH CENPB-type" evidence="3">
        <location>
            <begin position="666"/>
            <end position="741"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8033094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1064" mass="121766" checksum="5E188833D8D8795F" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BVP3</accession>
    <name>A0A9Q1BVP3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Netrin receptor UNC5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_20899</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Receptor for netrin required for axon guidance. Mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the unc-5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="427518at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005042">
        <property type="term" value="F:netrin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01670">
        <property type="entry name" value="Death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01120">
        <property type="entry name" value="RecA-like_superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.220.30">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000488">
        <property type="entry name" value="Death_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037936">
        <property type="entry name" value="UNC5A-D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000906">
        <property type="entry name" value="ZU5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12582">
        <property type="entry name" value="NETRIN RECEPTOR UNC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12582:SF41">
        <property type="entry name" value="UNC5C-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00531">
        <property type="entry name" value="Death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00791">
        <property type="entry name" value="ZU5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00005">
        <property type="entry name" value="DEATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50017">
        <property type="entry name" value="DEATH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51145">
        <property type="entry name" value="ZU5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="1" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="1 4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ZU5" evidence="3">
        <location>
            <begin position="1096"/>
            <end position="1235"/>
        </location>
    </feature>
    <feature type="domain" description="Death" evidence="2">
        <location>
            <begin position="1701"/>
            <end position="1784"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU367033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8034173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1790" mass="204355" checksum="93899E8E043B62B4" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BWW3</accession>
    <name>A0A9Q1BWW3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">FAM234A/B beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_21012</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BWW3"/>
    <dbReference type="OrthoDB" id="567787at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055409">
        <property type="entry name" value="Beta-prop_FAM234A_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045232">
        <property type="entry name" value="FAM234"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028994">
        <property type="entry name" value="Integrin_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21419">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21419:SF36">
        <property type="entry name" value="PROTEIN FAM234A-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23727">
        <property type="entry name" value="Beta-prop_FAM234A_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69318">
        <property type="entry name" value="Integrin alpha N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="65"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="FAM234A/B beta-propeller" evidence="7">
        <location>
            <begin position="125"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="598"/>
            <end position="619"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8034255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="619" mass="68695" checksum="830683E3A87672E3" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BY05</accession>
    <name>A0A9Q1BY05_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_21970</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BY05"/>
    <dbReference type="OrthoDB" id="10437383at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="147"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8034937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="204" mass="23750" checksum="B4960DD60607C5CD" modified="2023-09-13" version="1">MESTLETFKLLRRLYEKRVRYRHHLTNYKTYRANNSVPKGLQIHVPTGLPLCDSGSYSNWKGILRKTSLKLMEVQIGNCRKTISELNYQIDSKTRKLKRELPKSELQNKLRLISQDCLRLSHSLNSRQQRKFQRDQITSNQVILTSNQVSPNSKKSRSRRFLRKPATDSPTVTNAVVTISCQLSEEENQTSFRRDSILPQTKAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BY61</accession>
    <name>A0A9Q1BY61_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Diacylglycerol kinase</fullName>
            <shortName evidence="11">DAG kinase</shortName>
            <ecNumber evidence="11">2.7.1.107</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HOLleu_19285</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Nanhai2018</strain>
            <tissue evidence="16">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a 1,2-diacyl-sn-glycerol + ATP = a 1,2-diacyl-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10272"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.107"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the eukaryotic diacylglycerol kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.107" evidence="11"/>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8035568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BY61"/>
    <dbReference type="OrthoDB" id="242257at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004143">
        <property type="term" value="F:ATP-dependent diacylglycerol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20851">
        <property type="entry name" value="C1_DGK_typeI_like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000017">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000013">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000016">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10330:FF:000003">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.110">
        <property type="entry name" value="Diacylglycerol kinase alpha"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029477">
        <property type="entry name" value="DAG_kinase_typeI_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037607">
        <property type="entry name" value="DGK"/>
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    <dbReference type="InterPro" id="IPR038199">
        <property type="entry name" value="DGK_typeI_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000756">
        <property type="entry name" value="Diacylglycerol_kin_accessory"/>
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    <dbReference type="InterPro" id="IPR001206">
        <property type="entry name" value="Diacylglycerol_kinase_cat_dom"/>
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        <property type="entry name" value="EF-hand-dom_pair"/>
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        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11255">
        <property type="entry name" value="DIACYLGLYCEROL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11255:SF48">
        <property type="entry name" value="DIACYLGLYCEROL KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14513">
        <property type="entry name" value="DAG_kinase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00609">
        <property type="entry name" value="DAGK_acc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00781">
        <property type="entry name" value="DAGK_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00045">
        <property type="entry name" value="DAGKa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00046">
        <property type="entry name" value="DAGKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="7 11 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="EF-hand" evidence="15">
        <location>
            <begin position="248"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="13">
        <location>
            <begin position="343"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="13">
        <location>
            <begin position="423"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="domain" description="DAGKc" evidence="14">
        <location>
            <begin position="532"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="114"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="499"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="852"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="123"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="853"/>
            <end position="862"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU361128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50146"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAJ8035568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="873" mass="97011" checksum="F080C08F831D0A2E" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BY67</accession>
    <name>A0A9Q1BY67_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_21697</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome 10"/>
    </dbReference>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAJ8034734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="68" mass="8272" checksum="E8749743F7DF43F7" modified="2023-09-13" version="1">MSRQAKRQKQRFLEKEERARTDLESTEEGNQPKSSWRSYYIHRQGRRRTDYGIRKPPESTRTDAETRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BY83</accession>
    <name>A0A9Q1BY83_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Vacuolar protein sorting-associated protein 13C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_19326</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the VPS13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8035598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="428159at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045053">
        <property type="term" value="P:protein retention in Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="VPS13"/>
    </dbReference>
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        <property type="entry name" value="VPS13-like_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026854">
        <property type="entry name" value="VPS13_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16166:SF93">
        <property type="entry name" value="INTERMEMBRANE LIPID TRANSFER PROTEIN VPS13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16166">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN VPS13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25033">
        <property type="entry name" value="VPS13_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12624">
        <property type="entry name" value="VPS13_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Chorein N-terminal" evidence="4">
        <location>
            <begin position="22"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="Chorein N-terminal" evidence="4">
        <location>
            <begin position="199"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="domain" description="VPS13-like middle region" evidence="5">
        <location>
            <begin position="1003"/>
            <end position="1393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="384"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="758"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1268"/>
            <end position="1313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="384"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="759"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="774"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="791"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1268"/>
            <end position="1309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF25033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8035598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1398" mass="154311" checksum="D5516775E66C3A33" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BYZ4</accession>
    <name>A0A9Q1BYZ4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Tyrosine-protein kinase receptor Tie-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_22322</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BYZ4"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050198">
        <property type="entry name" value="Non-receptor_tyrosine_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24418">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="31"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8035180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="273" mass="31613" checksum="866AB3A60C04A4DD" modified="2023-09-13" version="1">MFEEVLDEKSSFTYQCDVSTFTGLYQKHVSTTYTTEVGQPVRCELKMKEQKTKCDSHSFHTALISRGNIQMKVIVKVFSEIASKCGERLHIEELQALETLETHESVARILGSCSTSSMLYLHTSLIWELHPSVFILAPYCIIFEFLAETLHENLKALYQSNECLHLNDSDEKTQESLKKFSCDVANGLEYLARKNFTYPRLTCQNIFLDDYKHCKLHDFTLRELHVNDLPGMHEQDDMQIAHEFLSNGDNTAKRDVWNYGQLLLKIYTFGWSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C0C5</accession>
    <name>A0A9Q1C0C5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_19719</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8035900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C0C5"/>
    <dbReference type="OrthoDB" id="2019149at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030417">
        <property type="entry name" value="MS4A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23320:SF165">
        <property type="entry name" value="MARVEL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23320">
        <property type="entry name" value="MEMBRANE-SPANNING 4-DOMAINS SUBFAMILY A MS4A -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="83"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="108"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="151"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8035900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="224" mass="24465" checksum="1BBE9518D44C1744" modified="2023-09-13" version="1">MQPSNRQVFVSQPYLVVTPSVTIIQTPSKEKLSGYKARSITGGIQIICGLLEIAFGIAVIIIPLRYSVLLFGTRDANGDNVGWGIWNGVFAVVTGVLGCCSRRYKSVVVYYMLTSIIATVSSVFCLIWGVLHISRANRALPDYDRVVNLAMYSVLSIAFAVHMVTSLIGAIFCCSATSYTNTPHQTVYSVSQMPLQQAGPLTHCNYPRTPEPPPPYIPLPPSYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C227</accession>
    <name>A0A9Q1C227_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_17627</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8036952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C227"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="112"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="146"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8036952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="291" mass="30016" checksum="CC352FFB753EAEE6" modified="2023-09-13" version="1">MKLGERPKLAVVITVVLVVVSEIVVKFVVVGEVDFIASSVGISVVVNFVVVDSGCDVVCTFNVVAMVVIEDVDGGVVELAVLIIVVLAVSSEIVVTFVTVGEIDFVESSVDISLVVNIVVVDSGCDVVCASNVVAMVVIKDADGGVVELAVLIIVVLAVVGEIDFVESSVDISLVVNIVVVDSGCDVVCASNVVAMVVIKDVDGRDDFVVSSVDISLAVNIVVVDSGCDVVCASNVVAMVVIKDADGGVVEATTSPSTSAPTGIKPDWELNGIRNKYCLSVVVIHLITRNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C2A9</accession>
    <name>A0A9Q1C2A9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Dehydrogenase/reductase SDR family member 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_18126</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C2A9"/>
    <dbReference type="OrthoDB" id="47007at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004090">
        <property type="term" value="F:carbonyl reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43943">
        <property type="entry name" value="DEHYDROGENASE/REDUCTASE (SDR FAMILY) MEMBER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43943:SF2">
        <property type="entry name" value="DEHYDROGENASE_REDUCTASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8037331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="304" mass="33615" checksum="BAC3C77D15BACFE7" modified="2023-09-13" version="1">MSSIPMLRQSRSLFLRGTSRVTDLHKRMMALCAYDDEYLKARFAGKVAICTASTSGIGYAAAERIAREGAKVVICSRRQEKVDAAVKKLRDQNLEVSGIVCHQAKKEDRQRLIDFTLRTYGGIDLLFASAGVSPFSGYMLLVTEKQWDKLFEVNVKSTFLIIRECVPHMAERGGGAIVSHSTTAGVFPEATRNLNMPYAVSKSAVIGLTKVLVEPLAQRNIRINCMAPGPIETSFLRRYIQQFVPGETGLPKQAYDENFMLRFGEPEECGSVVAFLLSEDASYMAGEIIPVCGGINLREMSKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C2C2</accession>
    <name>A0A9Q1C2C2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_18790</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C2C2"/>
    <dbReference type="OrthoDB" id="10058156at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF13">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="68"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="27"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="82"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="92"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8037863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="373" mass="42089" checksum="89D2BDCBE802CCA1" modified="2023-09-13" version="1">MEMAAKNVKEFCATVTSNSASINIQKSYIRSHKPTQGRGKAQGSKDHPPYRCNGPHSPKDCTFKNAECYNCHKKGHIAAACKSKPRHKGKDGRKPRSGEVHEIEDDTEQVPDEMTLYCIYSADSVNHFETKVMINDCPLNMRIDTAADCSIMSKDTYEQNFSNLPLKESTLQLKTYSSEILDTCGQIECKVVYNGKTFTLPCVAARYNKPTLMGKDWLSKLKLDWSSVFHIERTKLGQVLKEYSSLFEDSYEGIKGTVAHIRNKEDAKPKYCKARPVPYSQKSQVETELKRLEKNGVIVKTNYSDWATPIVIVPKTDGTVRLCGDYKITINQAVDDEKYPLPTSQDLYAQLSGTSVFKVRLVSCILSTQHRSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C2U1</accession>
    <name>A0A9Q1C2U1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_19165</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8038163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C2U1"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23215:SF0">
        <property type="entry name" value="BUB3-INTERACTING AND GLEBS MOTIF-CONTAINING PROTEIN ZNF207"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23215">
        <property type="entry name" value="ZINC FINGER PROTEIN 207"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="70"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="219"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="77"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="112"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="134"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="153"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="194"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="227"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="245"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8038163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="354" mass="37154" checksum="A027B1F1606D0BCD" modified="2023-09-13" version="1">MTQGMMPPVPLPGMPPVPMPPRGMPMPGMPNLPPFVPPPIPGMPMPPLPGMPGMPPLPRMVRMPQMNMGNDPRIPQPRTTAPSIPTSKPVVPLGPQVGPSVLGGQPPKPLFPSAASTSVTSSGSGPVGADFKPLQSSAPSTTSTIQTKPTFPSYSAPPISSTGSSSTTTTATTSAPTESRTKSTISTSATSKIIHPDEELSLEEIRSREPKYAAKPVVLPPTSSVAPMSASQQTQPSPQPPASSMPPTSSMGMPPTSSMVMPPTSSMGMPPRMSAGGMNMGHMPPGMLEHTRPPPMPMMGGMNPQHQPPMGNMPPMPMMFPMPPRVDRPPFGMPPQMMDRMPFRGPHPMQGGRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C3P3</accession>
    <name>A0A9Q1C3P3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Arylsulfatase G</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_19041</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sulfatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8038066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C3P3"/>
    <dbReference type="OrthoDB" id="103349at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004065">
        <property type="term" value="F:arylsulfatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050738">
        <property type="entry name" value="Sulfatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024607">
        <property type="entry name" value="Sulfatase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000917">
        <property type="entry name" value="Sulfatase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42693">
        <property type="entry name" value="ARYLSULFATASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42693:SF42">
        <property type="entry name" value="ARYLSULFATASE G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00884">
        <property type="entry name" value="Sulfatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14707">
        <property type="entry name" value="Sulfatase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00523">
        <property type="entry name" value="SULFATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Sulfatase N-terminal" evidence="7">
        <location>
            <begin position="19"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8038066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="517" mass="57004" checksum="F4F3D9C70FFC6A70" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C3S2</accession>
    <name>A0A9Q1C3S2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ARM REPEAT PROTEIN INTERACTING WITH ABF2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_18408</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C3S2"/>
    <dbReference type="OrthoDB" id="684045at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="CDD" id="cd14733">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18186">
        <property type="entry name" value="BTB_POZ_ZBTB_KLHL-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037189">
        <property type="entry name" value="HBS1-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24413">
        <property type="entry name" value="SPECKLE-TYPE POZ PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109732">
        <property type="entry name" value="HBS1-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="1">
        <location>
            <begin position="669"/>
            <end position="737"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8037564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="947" mass="107610" checksum="ADA3D95724A46EB4" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C562</accession>
    <name>A0A9Q1C562_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF5641 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_15849</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C562"/>
    <dbReference type="OrthoDB" id="8958038at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040676">
        <property type="entry name" value="DUF5641"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331:SF1">
        <property type="entry name" value="GAG-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18701">
        <property type="entry name" value="DUF5641"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF5641" evidence="1">
        <location>
            <begin position="24"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8038423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="84" mass="9800" checksum="1C0EEFB8D5D57B0E" modified="2023-09-13" version="1">MLLGRASQEVPQGPFRETRNPKHRVEFVQKIVNSFWERWTRDVLSLLVPRRKWDANRRNVCVNDIVMLVDSSAVRGKWTVGIPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C590</accession>
    <name>A0A9Q1C590_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BRICHOS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_15904</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C590"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8038468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="237" mass="26174" checksum="25C59F8111345A6E" modified="2023-09-13" version="1">MNKASRDTHVKVLGMVLVVAIIMTGFVGVVHLLSPSGASKIYADSQDGKSRQDADTAKQQDAVVNFDMSGKRHEEHVSVRNGGQLVTISDTEDGFSVILDYKDGIAVVKNMSSLECYFFPIEKFPEYQTSDMESLKPVVVQSNGENPDKNTGDEKSVTSLVKSGVIPWEFIKTTSDPTVAESCMDSTSYWLEGTTESHVQKRQAGDVCICWVVIEYTNGDVILYWWYCDCATGDPLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C629</accession>
    <name>A0A9Q1C629_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_16463</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C629"/>
    <dbReference type="OrthoDB" id="10507652at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8038903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="109" mass="12532" checksum="52E0BB64E33C5EAE" modified="2023-09-13" version="1">MASTKPSEEHYSDCRKVSLEEIEFLKEECERMIPLVKDSQADNLDATSISSDKYKELPGIKTTRRDGVFQTSQGRRHHSEPYPVAEDRSHSDSVFSYRYQDDSDSTRAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C649</accession>
    <name>A0A9Q1C649_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tyr recombinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_16414</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C649"/>
    <dbReference type="OrthoDB" id="5963861at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="entry name" value="Intergrase catalytic core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010998">
        <property type="entry name" value="Integrase_recombinase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35617:SF3">
        <property type="entry name" value="CORE-BINDING (CB) DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35617">
        <property type="entry name" value="PHAGE_INTEGRASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47823">
        <property type="entry name" value="lambda integrase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyr recombinase" evidence="3">
        <location>
            <begin position="188"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8038859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="378" mass="43408" checksum="80FF759EE1A7793E" modified="2023-09-13" version="1">MWGSYFFYAFPPFSLIGKSLQKVEQDQATGIFIAPLWATQTWFVKLMELLIDQPKAHSMSHYREALRQQGISEQAIEIILSAWRPSTQRQYASSFSRWFLFCREKDCSPFITTIPLILDFLTSLYNAGNGYSNINTARSMLSSFLICPHQIGKNYLIKRFMKGIFELRTPVPRYKTIWDVSIVLDYLRLLGETNTLPLKLLTYKVVTLIALISAQRCQTLGLLNLNSMEKKSDMFIFYLSDKVKQSRPGNVGLVVYTKFPGNELCAFTALERYLHLTKSLRKDSKLFISFVRPHASVSRETISRWIKYVLKESGLNTDLFKPHSTRSAATSGAFVRGVPVEDILQTAGWSNELSFVRFYQKPITDLKDNKFVESILTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C708</accession>
    <name>A0A9Q1C708_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Zinc finger MYM-type protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_17082</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C708"/>
    <dbReference type="OrthoDB" id="10063284at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025398">
        <property type="entry name" value="DUF4371"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45749">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45749:SF21">
        <property type="entry name" value="DUF4371 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14291">
        <property type="entry name" value="DUF4371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4371" evidence="1">
        <location>
            <begin position="10"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8039383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="187" mass="21645" checksum="A7250DF596553770" modified="2023-09-13" version="1">MQVKGHEMQVKKNHRFLEEVIITILFLARQGLAFRGHREGDDSDNRGNYLELLQLRARDNALLREGLNNQTYTSPVIQDKIISILGMEVRNIIVQEIQSMKPSFYTIMMDETRDISRKEQVSLCVRYVTKDMKVHERFLWFEEASSTTGEALYSLLKGKVKEYGMHMESIIVSLDSVMMVAVICLVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C749</accession>
    <name>A0A9Q1C749_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Alpha-tectorin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_17164</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C749"/>
    <dbReference type="OrthoDB" id="5273213at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="CDD" id="cd19941">
        <property type="entry name" value="TIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025615">
        <property type="entry name" value="TILa_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37860:SF1">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37860">
        <property type="entry name" value="AGAP008810-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12714">
        <property type="entry name" value="TILa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="2">
        <location>
            <begin position="192"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="256"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8039443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="430" mass="47765" checksum="B85ACEC6EB01C5D5" modified="2023-09-13" version="1">MSFLGVCRMNENRHQPKQYPLEATSCLNNQEKSGAGICRFAREFPSKVNWTSLFLKDPAGGKQKVASSGEITTVQSICPLVEVNCTICQGTCRNPNGCPSSNCTKLETCRCNDGFLLKGGECVPEDECGCFIEGEGVLPEGGKYTNENCSEQCVCEEGLLTCNNTCDTNAECRAKNGVRNCYCNDGFKGDGQTCTALPKDCYEAYQTGHRQNGVYTILPKGWPGSAFEVFCDMNRGGWTVMTNSWFRSVWCYNNDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDVDYDDDDDDDDDDDDDDDDDDDDDDDDVDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDGDDGDDGDDGDDGDDGDDGDDDDDDDDDDDDDDDDDEDDDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C7Y3</accession>
    <name>A0A9Q1C7Y3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_14194</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C7Y3"/>
    <dbReference type="OrthoDB" id="3598281at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8040012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="232" mass="26954" checksum="6F1779CCDE9A8823" modified="2023-09-13" version="1">MLEHLTELLHCASMLDVEVENLLEEWGMTELLKPFEDNGIDMEALLLLDEPSICELVPKIGLRVKFLTKWKAYVSFIYLEHDDDDCIITHEDQDEPDEDAINEIVLSFLATLRSYNSIPFSAVNEIIAQIEQDFQILYPEAAEKLYEKWPTLHQTMTVYADKNFPNWKEQLPWNCSHLDEEEQAFLSLPFVFQSRHKRKKGIGSASLTSSIRSFIDINPVSYLSVYSFSRKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C8Q3</accession>
    <name>A0A9Q1C8Q3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Receptor-type tyrosine-protein phosphatase T</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_14422</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10253954at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00055">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="CDD" id="cd00047">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12087">
        <property type="entry name" value="TM_EGFR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.300.10">
        <property type="entry name" value="Tie2 ligand-binding domain superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002049">
        <property type="entry name" value="LE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26391">
        <property type="entry name" value="INACTIVE TYROSINE-PROTEIN KINASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26391:SF18">
        <property type="entry name" value="PROTEIN KINASE RECEPTOR TIE-1, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="13" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1275"/>
            <end position="1297"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="139"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="8">
        <location>
            <begin position="249"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="12">
        <location>
            <begin position="534"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="12">
        <location>
            <begin position="641"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="12">
        <location>
            <begin position="745"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="12">
        <location>
            <begin position="854"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="12">
        <location>
            <begin position="958"/>
            <end position="1061"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="12">
        <location>
            <begin position="1066"/>
            <end position="1164"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="9">
        <location>
            <begin position="1363"/>
            <end position="1647"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="10">
        <location>
            <begin position="1567"/>
            <end position="1638"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="266"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8040194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1679" mass="189132" checksum="D3096FB4587A60B1" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C9F5</accession>
    <name>A0A9Q1C9F5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Sulfotransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_14799</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C9F5"/>
    <dbReference type="OrthoDB" id="10010208at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053259">
        <property type="entry name" value="Golvesin-related_Golgi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32301">
        <property type="entry name" value="COUNTIN RECEPTOR CNR3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32301:SF6">
        <property type="entry name" value="GOLVESIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8040491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="384" mass="44144" checksum="DF5BBE262DE6364E" modified="2023-09-13" version="1">MKNTPAASRYFLGGRDVTVFFIILATCIFVMVITLSPNEPLDSLPEMHSQSRTEIANLDSKKSDFFPLERFYPEDGNCLPNYFLHGDSVPDKRAYSDGVIGFQHLNKAGGMTTRACIRDFFTGPPYKMNYLISSPQRSELEAHMAFGSVSTDDIDIFHGDYFFGVCQKLKQGKLNRSCSYFTVLRDPYDRTISSYLYCQKANLDQTCGPFRVSDLTIEEWAILQGSYFFLQLLQKYEYCKMGDPSLNEFADILSENLPQTSYFGFPHHCWYLEKIILNKTLTLDGRRKLLDYLVSNLENIFAVIGLSDDFRSTFKLLEKVYKLPFKKCIKQSAKNLGPQSKNKTLYSSLKERLLRNPVVTDALKEDVELFRKGKEIFDKQMKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CD21</accession>
    <name>A0A9Q1CD21_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Short-chain dehydrogenase/reductase 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Retinal short-chain dehydrogenase/reductase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_13483</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the reduction of all-trans-retinal to all-trans-retinol in the presence of NADPH.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8042429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CD21"/>
    <dbReference type="OrthoDB" id="10253736at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052650">
        <property type="term" value="F:all-trans-retinol dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05339">
        <property type="entry name" value="17beta-HSDXI-like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000131">
        <property type="entry name" value="Short-chain dehydrogenase/reductase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057326">
        <property type="entry name" value="KR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24322">
        <property type="entry name" value="PKSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24322:SF746">
        <property type="entry name" value="SHORT CHAIN DEHYDROGENASE_REDUCTASE FAMILY 16C MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00822">
        <property type="entry name" value="PKS_KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Ketoreductase" evidence="13">
        <location>
            <begin position="41"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00059620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00068717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00082544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ8042429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="316" mass="35425" checksum="0643073A5920336C" modified="2023-09-13" version="1">MLGIFIKLPLVLLRSLFETLFAVVKRLVPLWLWRKKSVEGDIVLITGAGSGIGRQIALNFAQERAVLVLWDVDKFGNEETAKLVGQLNAEVYTYTCDVTKRENVYKLARKVQQEVGPVSILVNNAGIVSGKYFLDLKDESIIKTMEVNTLAHFWTIKAFLPDMVRRNHGHIVTISSVVGDGGVAGLSDYSASKCAVKGMHDALLREMHVSKSDVKCTVICPYGVSTGMFDGAQTRFEFLTPFITPEYAGKKIVQGILTERDVLYVPQDWQILVYLKHILPVNAQLVLEDFINANEAMMSFVGRRPAKKQRKNHHME</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CE06</accession>
    <name>A0A9Q1CE06_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Mediator of RNA polymerase II transcription subunit 19</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Mediator complex subunit 19</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">MED19</name>
        <name evidence="8" type="ORF">HOLleu_10775</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the Mediator complex subunit 19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CE06"/>
    <dbReference type="OrthoDB" id="10044050at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019403">
        <property type="entry name" value="Mediator_Med19_met"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22536">
        <property type="entry name" value="LUNG CANCER METASTASIS-RELATED LCMR1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22536:SF1">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10278">
        <property type="entry name" value="Med19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 6" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="145"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="29"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="153"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="188"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU364151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8043601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="227" mass="25082" checksum="9E71ABCC99839BC2" modified="2023-09-13" version="1">MKRSRSPGEMLPNLDGIPPKIPIPIRQKSPATPRTYHTQSNQDNEPFYLLKEISAEKTQVTGEQNLINHFGLQHSYDKFSSKKVKEQLSAFLPGLPGRIDTPGIQDNSSLQSVIEKPPIGGKPLIPLSGPALSGFKLHPGPVADHLKWLHSQPPKKPKRKKQRRDFGPPEADPNVGGVSEAGGDDSKKKKNREKKRGKKDKKKKKNKSSPKHTTMEQPHSAGGPVRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CEJ4</accession>
    <name>A0A9Q1CEJ4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Myosin light chain kinase, smooth muscle</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_10215</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CEJ4"/>
    <dbReference type="OrthoDB" id="2575524at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="15"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="43"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8043220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="111" mass="11772" checksum="778ED74AE324627A" modified="2023-09-13" version="1">MFITRIEPHTHTHTDNNPNTPQTNLPPATLKTNPHKDHTPKDNTTPKGNTTPEGNTTPNGNTKPEGNTTPEGNTKPEGNTTPEGNTKPEGNTTPEGNTTPEGNTTPEGNST</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CEY2</accession>
    <name>A0A9Q1CEY2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Dehydrogenase/reductase SDR family member 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_11466</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8044096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CEY2"/>
    <dbReference type="OrthoDB" id="1933717at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000047">
        <property type="entry name" value="NADP-dependent L-serine/L-allo-threonine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43115">
        <property type="entry name" value="DEHYDROGENASE/REDUCTASE SDR FAMILY MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43115:SF4">
        <property type="entry name" value="DEHYDROGENASE_REDUCTASE SDR FAMILY MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8044096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="269" mass="29185" checksum="1BD3A92977EB587B" modified="2023-09-13" version="1">MKTSARNIDGTVPKSCVIRMHRWIGRVAMVTGAANGVGAATVKLLVESGMKVAAVNCTTDFEKCEALATDLNKSKSERCLLPVECDVSKEDQVMQMFKKIKSEFGRLDVCVNSAGVGPPKGQLIEGSTEAWNQILDVNILGLSLCTREAIKLMQERGNEEGHVIHIGSYSGHRIPGMHVMHMYSGSKHAVRALTEGLRRELRSLNSNIRISSISPGAIDTPFGKGAIPDLGTRAALSAEDVAYQVLHCLQQPQHVQIQDIVILPTYQPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CF83</accession>
    <name>A0A9Q1CF83_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Aldose 1-epimerase</fullName>
            <ecNumber evidence="13">5.1.3.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HOLleu_07053</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Nanhai2018</strain>
            <tissue evidence="17">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Mutarotase that catalyzes the interconversion of beta-D-galactose and alpha-D-galactose during galactose metabolism. Beta-D-galactose is metabolized in the liver into glucose 1-phosphate, the primary metabolic fuel, by the action of four enzymes that constitute the Leloir pathway: GALM, GALK1 (galactokinase), GALT (galactose-1-phosphate uridylyltransferase) and GALE (UDP-galactose-4'-epimerase). Involved in the maintenance of the equilibrium between the beta- and alpha-anomers of galactose, therefore ensuring a sufficient supply of the alpha-anomer for GALK1. Also active on D-glucose although shows a preference for galactose over glucose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>alpha-D-galactose = beta-D-galactose</text>
            <dbReference type="Rhea" id="RHEA:28675"/>
            <dbReference type="ChEBI" id="CHEBI:27667"/>
            <dbReference type="ChEBI" id="CHEBI:28061"/>
            <dbReference type="EC" id="5.1.3.3"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="2">
            <dbReference type="Rhea" id="RHEA:28677"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>alpha-D-glucose = beta-D-glucose</text>
            <dbReference type="Rhea" id="RHEA:10264"/>
            <dbReference type="ChEBI" id="CHEBI:15903"/>
            <dbReference type="ChEBI" id="CHEBI:17925"/>
            <dbReference type="EC" id="5.1.3.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Carbohydrate metabolism; galactose metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5 13">Carbohydrate metabolism; hexose metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 13">Belongs to the aldose epimerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.3" evidence="13"/>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CF83"/>
    <dbReference type="OrthoDB" id="274691at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004034">
        <property type="term" value="F:aldose 1-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033499">
        <property type="term" value="P:galactose catabolic process via UDP-galactose, Leloir pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09019">
        <property type="entry name" value="galactose_mutarotase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.98.10:FF:000003">
        <property type="entry name" value="Aldose 1-epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018052">
        <property type="entry name" value="Ald1_epimerase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015443">
        <property type="entry name" value="Aldose_1-epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008183">
        <property type="entry name" value="Aldose_1/G6P_1-epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047215">
        <property type="entry name" value="Galactose_mutarotase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014718">
        <property type="entry name" value="GH-type_carb-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008277">
        <property type="entry name" value="PRK11055.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10091">
        <property type="entry name" value="ALDOSE-1-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10091:SF0">
        <property type="entry name" value="GALACTOSE MUTAROTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01263">
        <property type="entry name" value="Aldose_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005096">
        <property type="entry name" value="GALM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00545">
        <property type="entry name" value="ALDOSE_1_EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 13" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 13" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton donor" evidence="14">
        <location>
            <position position="174"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="14">
        <location>
            <position position="306"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="174"/>
            <end position="176"/>
        </location>
        <ligand>
            <name>beta-D-galactose</name>
            <dbReference type="ChEBI" id="CHEBI:27667"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>beta-D-galactose</name>
            <dbReference type="ChEBI" id="CHEBI:27667"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00006206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR005096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR005096-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR005096-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR005096-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ8044328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="342" mass="36730" checksum="228644CCCFB7679F" modified="2023-09-13" version="1">MITQTEFGTTSKGHSVSLFTLSNGTVSVKITNFGGIIVAIEVPDKHKQVADVVLGFDNVKGYEAKHPYFGATVGRVSGRIGQGKFTLNGEDYQLAINNGPNHLHGGLEGFDKKIFQATIENDSLKLVYVSADGEEGYPGEVTVTVVYSLGEDNGLVIDYHATSTKSTPISLTNHSYFNLAGHEAGSITDHVIQIPSDRFAESDVETLLATGTLLPVADSVFDLRQPVLIGDRIHDIPGGGFDVTYSNPNPSSDFVCARVEHPGSGRVLEVKTNQPGVHLYTGNFLDGSLTGKNGVHYPKHSALCLEMQNFPNAVNLPSFPSCILNPGEVYSSTTIYKFSLLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CFW8</accession>
    <name>A0A9Q1CFW8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_11624</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8044226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CFW8"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8044226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="56" mass="6543" checksum="ED320FF430DA7590" modified="2023-09-13" version="1">MYRDYPVNQRLPSESTRDCPFLQIYGCDCLVQIYILNCLVTSSAYLVEQLLPVLRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CFZ7</accession>
    <name>A0A9Q1CFZ7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_11688</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8044271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CFZ7"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040311262" evidence="1">
        <location>
            <begin position="22"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8044271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="303" mass="34435" checksum="8787C3709251A9AE" modified="2023-09-13" version="1">MAHKFLILISVVILWLQGVNSLEQKHLTDLREFRIQGLASKVEVPRSVVEDIFNNITTDSDWDLSLSKPESNNIFYDTHGTLFLGHAVIHDHTLSGLSCEMKERVAASYDTVNVALSFVSGGQHGIILLDLFTDSDKDVFFPYSTNWNHVHYIDQIEEAENGTITLRQGNDVMVLNTHHNYTCSKSQHYDVREEFNEITGESFTSTDYPLAFDWGCDVFGDEVDFCDILKQNEKDLKKAGSNVCTEMTITYQSKRCSATLAIQQMTGFFRNELPYFSAPSKRFEAEWVDREHGAGIVFPCFSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CHC7</accession>
    <name>A0A9Q1CHC7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">WSC domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_08358</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WSCD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CHC7"/>
    <dbReference type="OrthoDB" id="5985073at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051589">
        <property type="entry name" value="Sialate-O-sulfotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45964:SF9">
        <property type="entry name" value="SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45964">
        <property type="entry name" value="WSCD FAMILY MEMBER CG9164"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sulfotransferase" evidence="3">
        <location>
            <begin position="228"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="54"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="59"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8045362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="401" mass="45996" checksum="E4864D16A4A684A2" modified="2023-09-13" version="1">MLISRKFIKWKVLLCLGVTGVLVVLLHNKSHRHSDLDTFSDVAENFNLRRNGGSLKNSQKLEGDAQHFEEKSERDTNLSVRTNYSELSNRTKSELLAKVPLPLNSVTKRPILVESVKQNTSNRSVVDNIEKHAEVIQESGSQNLTDVKPPPRKAEYSDCATAKVMPPYTYPEYTAVVSAHGSGNTWARYLIERATGVSTGSYYHDPKLYNGGFLGEMSNPLHRNTIAVKTHHPFQRKDIVFQNAIVIVRDPFDVVLAERNRLVSQNGHTAVVSESYFKGPEWEKFARGHSRYWEHSCRECITIPNYLIVYYSDLKTNLERELRRMLKFLKVEPDEKRIACTLAFPEGKFRRPKKERTFDPFTKELIEEINGHIRNVSELFRINNIAPLPFQEKPLLGVFPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CJL4</accession>
    <name>A0A9Q1CJL4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Steroid 21-hydroxylase</fullName>
            <ecNumber evidence="14">1.14.14.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">21-OHase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Cytochrome P-450c21</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Cytochrome P450 21</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Cytochrome P450 XXI</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Cytochrome P450-C21</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">HOLleu_08854</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="24">Nanhai2018</strain>
            <tissue evidence="24">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="21">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 22">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.14.16" evidence="14"/>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CJL4"/>
    <dbReference type="OrthoDB" id="639466at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004508">
        <property type="term" value="F:steroid 17-alpha-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004509">
        <property type="term" value="F:steroid 21-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042446">
        <property type="term" value="P:hormone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042448">
        <property type="term" value="P:progesterone metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11027">
        <property type="entry name" value="CYP17A1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000049">
        <property type="entry name" value="steroid 21-hydroxylase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24289">
        <property type="entry name" value="STEROID 17-ALPHA-HYDROXYLASE/17,20 LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24289:SF20">
        <property type="entry name" value="STEROID 17-ALPHA-HYDROXYLASE_17,20 LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 21 22" id="KW-0349">Heme</keyword>
    <keyword evidence="10 21 22" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="24" id="KW-0456">Lyase</keyword>
    <keyword evidence="13 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 21 22" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0492">Microsome</keyword>
    <keyword evidence="11 22" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="9 22" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="23" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="21">
        <location>
            <position position="466"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00044040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00044116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00044217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00044265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00044282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00044304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00044342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="KAJ8045774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="523" mass="58832" checksum="3D50BB29AB4A30EA" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CJT2</accession>
    <name>A0A9Q1CJT2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_04867</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CJT2"/>
    <dbReference type="OrthoDB" id="10001532at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="27"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8046251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="96" mass="11122" checksum="F87E53ADEA897B65" modified="2023-09-13" version="1">MGIYNEATQEAVAERFREESADRPPSQKKVPTKKPSSSSKKTEKKTRKTYADKCDEKFQGRISLLERPPWKPSSTHPHNLYPQLHIHLQDPKTYSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CK96</accession>
    <name>A0A9Q1CK96_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Mixed lineage kinase domain-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_05122</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CK96"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27001">
        <property type="entry name" value="OS01G0253100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27001:SF931">
        <property type="entry name" value="OS11G0664100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="171"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8046450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="446" mass="52033" checksum="8ADFAEEFFD7269F8" modified="2023-09-13" version="1">MDMMKVGARGDFAICSGRCYPTPVSHTPSAARVLHFDRTIKENMHSLLIDINEVFLRTKMFFEKLEKYESDTGIVMLVKRVVKYYEIEHKFKDFNRMLVDFEDDLQVKQKDIGFEEAIDTHLVVHTLRQEQQNRVADFENFKNFVMERFNEEKEHREFVEEHMRKLQFYERRNEKPLSEGNYWTIHTAVLDKEKVTVKRIRDVNDHNVQASFQKETVNLMRFSRGSIVRVIGMCDEEHEKFLVLEYMEKGDLSTLLHNSGEKISLSNKVTLATTAARAFYVMSHKLVHTSLRTEKFLVDKYYTAKLSGMRYAKTFSSARRYGSKKPVVDLTCYLAPELYQPRELSPEVDVYGFGIVLWEIFTQTKPFSTLAKEIGKEPTHAEVKKFVVHDKGEEYDVGSGPIETMVGEIIRDCRALDPKKRPSVSEILNRLEKVGNELEDEEDGCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CRP6</accession>
    <name>A0A9Q1CRP6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NLR family CARD domain-containing protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_02374</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="427518at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312">
        <property type="entry name" value="NACHT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312:SF2">
        <property type="entry name" value="NUCLEOTIDE-BINDING OLIGOMERIZATION DOMAIN-CONTAINING PROTEIN 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="697"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1005"/>
            <end position="1023"/>
        </location>
    </feature>
    <feature type="domain" description="NACHT" evidence="2">
        <location>
            <begin position="831"/>
            <end position="953"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8049580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1861" mass="210521" checksum="B5C18E945DAFD38D" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CT27</accession>
    <name>A0A9Q1CT27_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_03823</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CT27"/>
    <dbReference type="OrthoDB" id="7430131at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34239">
        <property type="entry name" value="APPLE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34239:SF2">
        <property type="entry name" value="TRANSPOSABLE ELEMENT P TRANSPOSASE_THAP9 CONSERVED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8050571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="102" mass="11906" checksum="DEF2B08E4462571D" modified="2023-09-13" version="1">MDLNQFRWDLIRPDVKPEYQNLCNNSNPVRTLLFGDDVLTQLKELSDASRVSRRLHRRRKPAGSRPFRGRGRAIYRSGQRPFLGRGKGPQRQSPNMARPFTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CTV2</accession>
    <name>A0A9Q1CTV2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">RNA-directed DNA polymerase from mobile element jockey</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_03639</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CTV2"/>
    <dbReference type="OrthoDB" id="10065856at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33332">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8050434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="288" mass="33878" checksum="0BD3515C24B525A6" modified="2023-09-13" version="1">MLVYINDLPDNISSEVRLFADDCVIYRQIKNNLDQVQLQKDLNTLSEWQNKWQMHFNTKKCFSMRITHSRNPKLFIYTLDKDILESTASHTYLGVDISNNLTWNRHINHITSSANRSLAFIRRNLYSCPRSVKANAYLTLVRPLLEYSSSVWDPYTVSLSNKVEQIQRRAARFVCNDYSPHSSVTKMLKDLGWDSLKVRRTVNRLAIFHKARLGLLALPMNNLQPVRRPSRHHHSNSFLHIPTNKDCYKYSFFPRTVRDWNLLPQNITDLEDPNQFKSAALRILRRDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H1T9</accession>
    <name>A0A9Q1H1T9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">P2X purinoceptor 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_26784</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H1T9"/>
    <dbReference type="OrthoDB" id="9898867at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046815">
        <property type="entry name" value="P2RX7_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36981:SF12">
        <property type="entry name" value="P2X PURINOCEPTOR 7-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36981">
        <property type="entry name" value="ZGC:195170"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20478">
        <property type="entry name" value="P2RX7_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="P2X purinoreceptor 7 intracellular" evidence="2">
        <location>
            <begin position="26"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8030374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="189" mass="21430" checksum="34E937AA452DF331" modified="2023-09-13" version="1">MADFSEQEGPQGYDFEPTMSEEQYRKKMAAAQHEETDSVGGEPELELEDERVGNTAWCQCGLCEAMETRVESICCTEIGNLEEKVMGTCVTLHRSFDSLCLNEDVLSATWNLLQHTKGNPPSENLQNRQYRYVAYKMFTQWGHGRMGKGNRTPLPSCAVYQIRLRFPEPDADYTGFSYADEVLADAMPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H2I8</accession>
    <name>A0A9Q1H2I8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_26495</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H2I8"/>
    <dbReference type="OrthoDB" id="2433005at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8030165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="462" mass="52423" checksum="D04027374F89289C" modified="2023-09-13" version="1">MDFKWMFVDEAFEDSSQLSHDFDYSWLFDDDQNHEPETKRPRGRPSLSSTHPDAVERAASFIQQNGFQADARRRGVTSRSSGVSVQDIADHVRNAVPALKETGVSKSTVRRWMKAPHEGRFASKRYEGLIDAKIPSKRNDFREENRDDHFHRAVVKYMREMCSHFQSECVVYSCDNMNKLKVGTLAVSRYHQLNRIYPSEDRPNYSDHDFPTPGYLIIPSGYMKLEPKVKKGTITKDSIGRDHIEYPVTGPLNIVLRAQPFAKATIETHMNDLKPLLKSDASRGQSFAVLIADGGPDWNITSPLTQIACMRLWKDCGLDGLVLTSFSAGNSAMNPIEHAWAPISKLLTGVTLPDKVPGESLPPCKQRNLSEDERKAKEEIVFNEAMGRVCQYLHGKQFDGHDINCSYVKCNAPQSGYKDHEELRQALVGPVGNLENHRLHNIRNDYTFAVKHMDRRLASLTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H2T7</accession>
    <name>A0A9Q1H2T7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Exonuclease mut-7-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_24553</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H2T7"/>
    <dbReference type="OrthoDB" id="18193at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002562">
        <property type="entry name" value="3'-5'_exonuclease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052408">
        <property type="entry name" value="Exonuclease_MUT-7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002782">
        <property type="entry name" value="Mut7-C_RNAse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47765">
        <property type="entry name" value="3'-5' EXONUCLEASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47765:SF2">
        <property type="entry name" value="EXONUCLEASE MUT-7 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01612">
        <property type="entry name" value="DNA_pol_A_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01927">
        <property type="entry name" value="Mut7-C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00474">
        <property type="entry name" value="35EXOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3'-5' exonuclease" evidence="2">
        <location>
            <begin position="399"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="608"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="789"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="622"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="790"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="818"/>
            <end position="828"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8031379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="991" mass="113316" checksum="E63411826738D0AC" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H2X2</accession>
    <name>A0A9Q1H2X2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_27649</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8031048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H2X2"/>
    <dbReference type="OrthoDB" id="8038710at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8031048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="79" mass="9482" checksum="AA434ACB7ADAFE65" modified="2023-09-13" version="1">MRCLRKIQGITWEDNLVTNCEVLSKAKLSTIFVMFSVRRLRWLGHVHRMEKVRIAKDLLYGQLERGSRSRGRPHLRYRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H2Y0</accession>
    <name>A0A9Q1H2Y0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">von Willebrand factor A domain-containing protein 8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_27041</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Exhibits ATPase activity in vitro.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="5186at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000587">
        <property type="entry name" value="von Willebrand factor A domain containing 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000663">
        <property type="entry name" value="von Willebrand factor A domain containing 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011704">
        <property type="entry name" value="ATPase_dyneun-rel_AAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039891">
        <property type="entry name" value="VWA8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21610">
        <property type="entry name" value="VON WILLEBRAND FACTOR A DOMAIN-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21610:SF9">
        <property type="entry name" value="VON WILLEBRAND FACTOR A DOMAIN-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07728">
        <property type="entry name" value="AAA_5"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="VWFA" evidence="9">
        <location>
            <begin position="1755"/>
            <end position="1960"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1423"/>
            <end position="1442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1423"/>
            <end position="1432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00055988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00070377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8030585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1975" mass="221199" checksum="876232C77383277D" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H3M6</accession>
    <name>A0A9Q1H3M6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_24964</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H3M6"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040266131" description="Ig-like domain-containing protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="28"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8031693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="146" mass="15933" checksum="33631FBAD9E028CC" modified="2023-09-13" version="1">MGKSSLLCLLAMTVVSFSVYGSCSDCSPGFDHNVEGILGRNVNLTCNMKYQCDSFRWDKPEQRGVISLSGESVQVGPSKSYVPISGIGFDDDGSYRCFCYTNGVNIGMCEVYLKTICQATVTLSGRMSVSKVKLLKRFLSVTSTFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H4R1</accession>
    <name>A0A9Q1H4R1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PB1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_22620</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H4R1"/>
    <dbReference type="OrthoDB" id="6512834at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8035397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="145" mass="15986" checksum="45C7231C07A10ED6" modified="2023-09-13" version="1">MDPCKLRVVITDSNIGKLYLQERPTSVEKLKDIIINKFDINYDFSLQYADSDFGGDMFSLTDINDLPRDIATIKVVAASHIPLDLSNVNYSDLSSASTDSVADSSHDTISISSSDDLQSLETKLQGSWPDVSQIPKLGPELELVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H6B1</accession>
    <name>A0A9Q1H6B1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_17933</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H6B1"/>
    <dbReference type="OrthoDB" id="2142683at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050111">
        <property type="entry name" value="C-type_lectin/snaclec_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22803">
        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040477700" description="C-type lectin domain-containing protein" evidence="2">
        <location>
            <begin position="32"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="3">
        <location>
            <begin position="42"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8037182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="167" mass="19418" checksum="828C7C274F61917B" modified="2023-09-13" version="1">MTGVSPPFHFDLHLIRLSIFLISLSTELTNSELRCHEQFIYFQNNCYYFSSQRLRFSAAKSVCNNYSDVSNPCHLTSIHSDCENKFVYQRATQLWGTATYWLGALKTTTGGNFSSWLDGSQMNFTQLKAYNKHGACLQQWTKENEWDDTFCSLKKYYVCKQTALCKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H6H0</accession>
    <name>A0A9Q1H6H0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_20942</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H6H0"/>
    <dbReference type="OrthoDB" id="10068393at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46601:SF1">
        <property type="entry name" value="ADF-H DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46601">
        <property type="entry name" value="ULP_PROTEASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8034201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="578" mass="66279" checksum="49E6224194C7159A" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H6I3</accession>
    <name>A0A9Q1H6I3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_21002</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
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    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome 10"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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            <begin position="41"/>
            <end position="62"/>
        </location>
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        <location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="42"/>
            <end position="56"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8034245.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
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    </evidence>
    <sequence length="116" mass="13367" checksum="9A96BC22A8D68911" modified="2023-09-13" version="1">MIKDLKWVDSLKTRRLRARLVVIYKETHGLLPSNVVDHLIDPSNPNRRTSSRTSGRYLHHHPSTNKLCYQKSLKKSLNHGHVLLRAQKRTGPDGVKHVSCRQPKYPILTPPSPQKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H720</accession>
    <name>A0A9Q1H720_HOLLE</name>
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        <submittedName>
            <fullName evidence="1">Zinc finger MYM-type protein 6</fullName>
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    </protein>
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        <name evidence="1" type="ORF">HOLleu_22292</name>
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    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
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        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome 10"/>
    </dbReference>
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        <property type="entry name" value="EPM2A-INTERACTING PROTEIN 1"/>
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        <property type="entry name" value="SCAN BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8035158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="194" mass="21867" checksum="FD9A1046EB51B4A8" modified="2023-09-13" version="1">MSAPKKKKKCRRYSFEYLTYGFIQSPTNKYLPMCFLCQTTMSNETMKLSRLSEHLGKKHSDKTGADVSYFQSLKENFENRSTITTMLKASQQRMDKGLEASYELLLLIAMSGKPHSIGEQLIKPAVGEVLKTVMGKDPNPELSSTALSNDSVARRIDNMSTDVDDKLCSELSNTHFTIQLDESTFRDSKALLLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-10-08" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H7U5</accession>
    <name>A0A9Q1H7U5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Protein prune-like 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_19341</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
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        </subcellularLocation>
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    <comment type="similarity">
        <text evidence="3">Belongs to the PPase class C family. Prune subfamily.</text>
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    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-KW"/>
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            <begin position="3621"/>
            <end position="3633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="3824"/>
            <end position="3833"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="3854"/>
            <end position="3878"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="3932"/>
            <end position="3946"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8035611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="4408" mass="491187" checksum="E1F9504C1A40B9BC" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H870</accession>
    <name>A0A9Q1H870_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Rieske domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_20018</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H870"/>
    <dbReference type="OrthoDB" id="426882at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00657">
        <property type="entry name" value="Ferritin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03467">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="entry name" value="Rieske [2Fe-2S] iron-sulphur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007402">
        <property type="entry name" value="DUF455"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054716">
        <property type="entry name" value="Sol_Rieske_ferrdox_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42782:SF2">
        <property type="entry name" value="3-OXOACYL-[ACYL-CARRIER-PROTEIN] SYNTHASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42782">
        <property type="entry name" value="SI:CH73-314G15.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04305">
        <property type="entry name" value="DUF455"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22543">
        <property type="entry name" value="Rieske_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="ISP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rieske" evidence="5">
        <location>
            <begin position="22"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8036135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="438" mass="48930" checksum="74F1B1949ACA7541" modified="2023-09-13" version="1">MDEDFPQDLVKALKEVVDSNRWQNIGAAEKFKTKKCTRLLSEDGEDMVIVFVAPDKFYAMDASCPHAGGPMNLGDIEDIGGHMSIICPWHGYSFRLDDGSSPTGLQQQMYTVKVLEGNIYVNTSSELLVPSSNTTENCEDSKEDISSAVVPSNADTLCDWAVRILMTADPHEKVQLTHKVKERWDSGDIKEIGSATPPSQPHRQDDLQVLAPGKIKRGKAGTKASRIAVLHSLANIEQWAIDLSWDVIARFSDISIGQSGKLPREFFTDFVKVASDEAKHFNLLQGRLKELGSHFGALPVHNGLWHSAGETKDSLLARLAIVHMVHEARGLDVHPITQARFARQNDTESVSLLEVIYKDEITHVAAGMKWFTYICKHSDPEMDCIPKFHELVRQKFRGHLKPPFNEEGRQTAGMTKEWYVPLMKPSEEDTIARKGTNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HA11</accession>
    <name>A0A9Q1HA11_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DNA-3-methyladenine glycosylase</fullName>
            <ecNumber evidence="4">3.2.2.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">3-alkyladenine DNA glycosylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">3-methyladenine DNA glycosidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">ADPG</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">N-methylpurine-DNA glycosylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HOLleu_18917</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Nanhai2018</strain>
            <tissue evidence="15">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of alkylated DNA, releasing 3-methyladenine, 3-methylguanine, 7-methylguanine and 7-methyladenine.</text>
            <dbReference type="EC" id="3.2.2.21"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">Binds MBD1. Binds SSBP1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DNA glycosylase MPG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.21" evidence="4"/>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HA11"/>
    <dbReference type="OrthoDB" id="6353017at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003905">
        <property type="term" value="F:alkylbase DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00540">
        <property type="entry name" value="AAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.300.10:FF:000001">
        <property type="entry name" value="Putative 3-methyladenine DNA glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.300.10">
        <property type="entry name" value="Methylpurine-DNA glycosylase (MPG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00527">
        <property type="entry name" value="3MGH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011034">
        <property type="entry name" value="Formyl_transferase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003180">
        <property type="entry name" value="MPG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036995">
        <property type="entry name" value="MPG_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00567">
        <property type="entry name" value="3mg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10429">
        <property type="entry name" value="DNA-3-METHYLADENINE GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10429:SF0">
        <property type="entry name" value="DNA-3-METHYLADENINE GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02245">
        <property type="entry name" value="Pur_DNA_glyco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50486">
        <property type="entry name" value="FMT C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="7"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00066187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00068926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00076879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00078171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00082988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ8037963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="256" mass="28773" checksum="A5D8A3C1934A2668" modified="2023-09-13" version="1">MKRRNLKTASKLSAKLSNDANPAKKQKMSKSPHVSGNTVSPYFQKTEQKRLGSAFYHQECIQLSKSLLGKVLVRKLNGKRLSGRIVETEAYLGIKDSACHSYKGHQSDNNKAMWMDPGYVYVYLIYGMYHCMNISGKGDGNAVLLRGLEPVDGEEVMQKLRSKRRKSSTELKRKALCSGPGKLTEALQITKALFDKESLVTNSEMWVEDAPVISDDDIVSCPRIGIDYATKEWKEKPLRFYIRGNACVSKRDKGAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HAF1</accession>
    <name>A0A9Q1HAF1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_15797</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HAF1"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8038383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="90" mass="10626" checksum="22E5F86D28251F9B" modified="2023-09-13" version="1">MKWKSSEQDEGNWYQEQAGEKRVKAEECGLGTYMPLQVRLNSDHVLSPYVREIDYLLSLAPRKENAEALRKPVWIRRRPVEHFHIFLAGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HC44</accession>
    <name>A0A9Q1HC44_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CST complex subunit STN1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Suppressor of cdc thirteen homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_14944</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HC44"/>
    <dbReference type="OrthoDB" id="77828at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015253">
        <property type="entry name" value="CST_STN1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040260">
        <property type="entry name" value="RFA2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13989:SF33">
        <property type="entry name" value="CST COMPLEX SUBUNIT STN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13989">
        <property type="entry name" value="REPLICATION PROTEIN A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09170">
        <property type="entry name" value="STN1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0779">Telomere</keyword>
    <feature type="domain" description="Stn1 C-terminal" evidence="9">
        <location>
            <begin position="213"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF09170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8040605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="328" mass="38358" checksum="77A72B023E3204AE" modified="2023-09-13" version="1">MYGNYQMLDCFSVLNVPTLIPINTNCNIYECDLNMCAIKSASTYPDCQMIPEMQWKIDPMYSSCCNLFIKDILELPLYTGSQENTFMYKNHPIRLVSLMGIVVKVEEKSQFFIYGVSVTSAQSLVDRMERWHLEQLTCLQVGDLIHLRGRLRMYRNAMQISVFSYGKVIDPTHSFEVTHMDSQITKYQRVYDVPFCTNSHLDKAMESRKRPKKSFKEHIHKSKNKNLAPQIVAVLKEAIKNLLHKGLIFKSKRMKGLHYLTLANKQLKKEVCHILKKDCQKKHYLNGCHFLHIYDTVINCDDFQCLTHVALKFAFKLFRARRSDSQFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HDG3</accession>
    <name>A0A9Q1HDG3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ras-related protein Rab-22A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_12937</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HDG3"/>
    <dbReference type="OrthoDB" id="63533at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01860">
        <property type="entry name" value="Rab5_related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000808">
        <property type="entry name" value="Small GTP-binding protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47978">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8041985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="227" mass="25306" checksum="27C5E1527AC17A15" modified="2023-09-13" version="1">MDINRIHCENGDKCMNGLHETSAGRISKQPHRMGSLREVKVCLLGDSGVGKSSIVQRFVTDTYHDNISPTIGASFMGKTLQVGNESFKFQIWDTAGQEKYRGLAPMYYRNAGAAIIVYDITNQSTFDLIKVWVRELHQHGPENICIAIAGNKCDLEDLREVTERDGAAYASEINAVFGETSAKTAKNISEIFIEISKNLPPLEFEKYKQGVIQVDSPKKNKRKGCCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HEA1</accession>
    <name>A0A9Q1HEA1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Crt-like 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_05046</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CRT-like transporter family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HEA1"/>
    <dbReference type="OrthoDB" id="6335830at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013936">
        <property type="entry name" value="CRT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31326">
        <property type="entry name" value="PROTEIN CLT2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31326:SF1">
        <property type="entry name" value="PROTEIN CLT2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08627">
        <property type="entry name" value="CRT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="42"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="78"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="118"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="142"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="171"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="213"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="245"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="303"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="330"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="362"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8046392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="406" mass="45554" checksum="65ED36F7E315BBBC" modified="2023-09-13" version="1">MGSTYKDDHHQPLLDSTSPIPVQESHQVFHLRGDRILRINKLTWNVFLTIITVLTSVLMNVTLPLYSNSMTKDGGSEYPVLLLSSMWFPFFFFGLVFLTKFLSPSMSLRSSVSNRIMILVGFLNAINGVLVVYASSTSRTPAALQAILSTSVIPFTVISRYILLRKGVSKARLVCTGIVLIGLFISLEPTIFNIDGNGGGGGGGEHLSNFEKFIWPVIFMFGFLPVGISNAVLERELKKGETESLLFLAWAQLYNFVFIAALFWTDFIPRFGASSSFHDFWENLTYGLKCQYGADQSCHSAVGASWIFILGYCSANLMIFLLVKYAEGAIYLVVVQAMVTPLGALFWTFFVPTPYFHWDPHFLQLSTFFIMLGILIMVPAVAMYNYFGIKEEKDLKRQLDLQSEDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HFS7</accession>
    <name>A0A9Q1HFS7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_07682</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HFS7"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8044819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="94" mass="10151" checksum="1685E12FF9B42D76" modified="2023-09-13" version="1">MVFGLGVAAATAALSYKFTRSFFGEQGQGTDSISPRREDRGCCCAETRVLPSSELLDDKGYNLVEDSKKAVCTCSCKPPECWTVSKTNKNESTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HFY2</accession>
    <name>A0A9Q1HFY2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">GTPase IMAP family member 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_00593</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. AIG1/Toc34/Toc159-like paraseptin GTPase family. IAN subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HFY2"/>
    <dbReference type="OrthoDB" id="8954335at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006703">
        <property type="entry name" value="G_AIG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045058">
        <property type="entry name" value="GIMA/IAN/Toc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903:SF184">
        <property type="entry name" value="GTP-BINDING PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903">
        <property type="entry name" value="GTPASE, IMAP FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04548">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51720">
        <property type="entry name" value="G_AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIG1-type G" evidence="4">
        <location>
            <begin position="53"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8048327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="254" mass="28151" checksum="D00068FFC9648876" modified="2023-09-13" version="1">MGFMKGAYSHSSGHCGRLKNIDPGKSQDVLASTTVALDKLIPGRFKHQPSNSSKNFTVVLVGKTGSGKSSFGNLLVGDSVFEVNSNAESVTTERQDASFKLGNSTVTVWDTPGFYDPKKNHVLALKELRRIYKGVREGIHTIALVARCESSVDAEKVYKKIKNKHPPDVLKYIILVLTHIKHQSVNTLLEDRFQNLAKEVKSRAIGVREESINPNNIEEQKALRESFLNIVQAVYEDNSNSPCTNLGTSFCRLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HGI8</accession>
    <name>A0A9Q1HGI8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Papilin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_08704</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HGI8"/>
    <dbReference type="OrthoDB" id="5950222at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00109">
        <property type="entry name" value="Kunitz-type"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd22630">
        <property type="entry name" value="Kunitz_collagen_alpha6_VI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.830:FF:000001">
        <property type="entry name" value="A disintegrin and metalloproteinase with thrombospondin motifs 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000005">
        <property type="entry name" value="ADAM metallopeptidase with thrombospondin type 1 motif 9"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.410.10:FF:000020">
        <property type="entry name" value="Collagen, type VI, alpha 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.410.10:FF:000005">
        <property type="entry name" value="Pancreatic trypsin inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.410.10:FF:000011">
        <property type="entry name" value="Tissue factor pathway inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.830">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.10">
        <property type="entry name" value="Pancreatic trypsin inhibitor Kunitz domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013273">
        <property type="entry name" value="ADAMTS/ADAMTS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050439">
        <property type="entry name" value="ADAMTS_ADAMTS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045371">
        <property type="entry name" value="ADAMTS_CR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010294">
        <property type="entry name" value="ADAMTS_spacer1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002223">
        <property type="entry name" value="Kunitz_BPTI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036880">
        <property type="entry name" value="Kunitz_BPTI_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020901">
        <property type="entry name" value="Prtase_inh_Kunz-CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723">
        <property type="entry name" value="ADAMTS A DISINTEGRIN AND METALLOPROTEASE WITH THROMBOSPONDIN MOTIFS PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723:SF281">
        <property type="entry name" value="PAPILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19236">
        <property type="entry name" value="ADAMTS_CR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05986">
        <property type="entry name" value="ADAMTS_spacer1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00014">
        <property type="entry name" value="Kunitz_BPTI"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19030">
        <property type="entry name" value="TSP1_ADAMTS"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00090">
        <property type="entry name" value="TSP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01857">
        <property type="entry name" value="ADAMTSFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00759">
        <property type="entry name" value="BASICPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00131">
        <property type="entry name" value="KU"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57362">
        <property type="entry name" value="BPTI-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00280">
        <property type="entry name" value="BPTI_KUNITZ_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50279">
        <property type="entry name" value="BPTI_KUNITZ_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8 9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0722">Serine protease inhibitor</keyword>
    <keyword evidence="5 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040138512" evidence="10">
        <location>
            <begin position="25"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="11">
        <location>
            <begin position="711"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="11">
        <location>
            <begin position="774"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="11">
        <location>
            <begin position="832"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="11">
        <location>
            <begin position="901"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="46"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="50"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="61"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR613273-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8045659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="957" mass="106144" checksum="169D8BA19221BF4F" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HGJ9</accession>
    <name>A0A9Q1HGJ9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">glutamate carboxypeptidase II</fullName>
            <ecNumber evidence="18">3.4.17.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">HOLleu_08759</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="23">Nanhai2018</strain>
            <tissue evidence="23">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>Release of an unsubstituted, C-terminal glutamyl residue, typically from Ac-Asp-Glu or folylpoly-gamma-glutamates.</text>
            <dbReference type="EC" id="3.4.17.21"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M28 family. M28B subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.17.21" evidence="18"/>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HGJ9"/>
    <dbReference type="OrthoDB" id="5841748at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016805">
        <property type="term" value="F:dipeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08022">
        <property type="entry name" value="M28_PSMA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02121">
        <property type="entry name" value="PA_GCPII_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000089">
        <property type="entry name" value="N-acetylated alpha-linked acidic dipeptidase like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.930.40:FF:000001">
        <property type="entry name" value="N-acetylated-alpha-linked acidic dipeptidase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.30:FF:000002">
        <property type="entry name" value="N-acetylated-alpha-linked acidic dipeptidase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.40">
        <property type="entry name" value="Transferrin receptor-like, dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039373">
        <property type="entry name" value="Peptidase_M28B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007365">
        <property type="entry name" value="TFR-like_dimer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036757">
        <property type="entry name" value="TFR-like_dimer_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10404:SF77">
        <property type="entry name" value="GLUTAMATE CARBOXYPEPTIDASE 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10404">
        <property type="entry name" value="N-ACETYLATED-ALPHA-LINKED ACIDIC DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04253">
        <property type="entry name" value="TFR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47672">
        <property type="entry name" value="Transferrin receptor-like dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0224">Dipeptidase</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="7 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="66"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="PA" evidence="20">
        <location>
            <begin position="228"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M28" evidence="22">
        <location>
            <begin position="408"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="domain" description="Transferrin receptor-like dimerisation" evidence="21">
        <location>
            <begin position="674"/>
            <end position="794"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00052003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00066561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF04253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KAJ8045704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="804" mass="90106" checksum="A09BA0DF2DD4AC6D" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HHE9</accession>
    <name>A0A9Q1HHE9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">DNA transposase THAP9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_03066</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HHE9"/>
    <dbReference type="OrthoDB" id="10070386at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021896">
        <property type="entry name" value="THAP9-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006612">
        <property type="entry name" value="THAP_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048366">
        <property type="entry name" value="TNP-like_GBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048367">
        <property type="entry name" value="TNP-like_RNaseH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048365">
        <property type="entry name" value="TNP-like_RNaseH_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47577:SF2">
        <property type="entry name" value="THAP DOMAIN CONTAINING 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47577">
        <property type="entry name" value="THAP DOMAIN-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05485">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21788">
        <property type="entry name" value="TNP-like_GBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21789">
        <property type="entry name" value="TNP-like_RNaseH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21787">
        <property type="entry name" value="TNP-like_RNaseH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12017">
        <property type="entry name" value="Tnp_P_element"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00980">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50950">
        <property type="entry name" value="ZF_THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="THAP-type" evidence="7">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="96"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8050037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="618" mass="69730" checksum="89D8FAC31AFD8296" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HI76</accession>
    <name>A0A9Q1HI76_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HOLleu_05403</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Nanhai2018</strain>
            <tissue evidence="17">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12 19">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 19">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
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    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1390">
        <property type="entry name" value="ROC domain from the Parkinson's disease-associated leucine-rich repeat kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
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        <property type="entry name" value="ANK_LRRK2"/>
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        <property type="entry name" value="Ankyrin_rpt"/>
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    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
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    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
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    <dbReference type="InterPro" id="IPR056597">
        <property type="entry name" value="ARM_LRRK2"/>
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    <dbReference type="InterPro" id="IPR056602">
        <property type="entry name" value="Beta-prop_LRRK2"/>
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        <property type="entry name" value="COR-A"/>
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        <property type="entry name" value="COR-B"/>
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    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
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    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
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    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011044">
        <property type="entry name" value="Quino_amine_DH_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020859">
        <property type="entry name" value="ROC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051420">
        <property type="entry name" value="Ser_Thr_Kinases_DiverseReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48005">
        <property type="entry name" value="LEUCINE RICH REPEAT KINASE 2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE DDB_G0278509-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23745">
        <property type="entry name" value="ANK_LRRK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23744">
        <property type="entry name" value="ARM_LRRK2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23748">
        <property type="entry name" value="Beta-prop_LRRK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16095">
        <property type="entry name" value="COR-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25497">
        <property type="entry name" value="COR-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08477">
        <property type="entry name" value="Roc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50969">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
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    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS51424">
        <property type="entry name" value="ROC"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="9 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Roc" evidence="16">
        <location>
            <begin position="1406"/>
            <end position="1593"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="1963"/>
            <end position="2225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="761"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="813"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="921"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="827"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="937"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="1990"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ8046650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="2624" mass="293972" checksum="188B2C6F8448FDB5" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HJU9</accession>
    <name>A0A9Q1HJU9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_05550</name>
        <name evidence="7" type="ORF">HOLleu_06740</name>
        <name evidence="5" type="ORF">HOLleu_20590</name>
        <name evidence="3" type="ORF">HOLleu_33506</name>
        <name evidence="4" type="ORF">HOLleu_33859</name>
        <name evidence="2" type="ORF">HOLleu_37808</name>
        <name evidence="1" type="ORF">HOLleu_43138</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000215">
        <property type="protein sequence ID" value="KAJ8018724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8026112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HJU9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8018724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8022810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8025841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8026112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8036573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8046765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8047683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="69" mass="7940" checksum="045CEC4CAA590E94" modified="2023-09-13" version="1">MESSREFNFNAAVLELSRKIVIIHHSSPSLVIIRSWNQFLRYSTLQVYYRTIVNVHALPALGPDQANIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HLS1</accession>
    <name>A0A9Q1HLS1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_03313</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HLS1"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8050206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="66" mass="7410" checksum="6134378F5AE5956E" modified="2023-09-13" version="1">MPCEKAFKLVKKMCKIAAVTVVRFVTLCTVHVCGLNNRKLRLSLPHKLLCPEVGIAQLLSWSFRLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YA80</accession>
    <name>A0A9Q0YA80_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_44685</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010001117">
        <property type="protein sequence ID" value="KAJ8017711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YA80"/>
    <dbReference type="OrthoDB" id="6151961at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37162:SF10">
        <property type="entry name" value="DUF4371 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37162">
        <property type="entry name" value="HAT FAMILY DIMERISATION DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8017711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="71" mass="7987" checksum="DA118A8835578BEC" modified="2023-09-13" version="1">MMGPQRGVASFVKREHENVFILGCPCHLIHLAAEKGANQLPFSPVSLLIPIFYYLAKSSKRNMDCRKINSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YAW0</accession>
    <name>A0A9Q0YAW0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CUB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_44115</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000648">
        <property type="protein sequence ID" value="KAJ8018086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YAW0"/>
    <dbReference type="OrthoDB" id="10520554at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8018086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="172" mass="18703" checksum="EB2C84E66CC66AD6" modified="2023-09-13" version="1">MSSFVLLFSATDFTVIPTPTSDADFTEACSETGTTVRLQYASESSNAILGSPTYFTGSGVLMSCVWTIIGPTGSYLAIIFRNFQLELAVDGFEIRYLDQRFAFGHTMYSSNVMNLPVTRAGTTLPLIGTPIILDSSRVRLTYDAQTSDLVSYPGPIFEVHVLGKGNVILFSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YCK5</accession>
    <name>A0A9Q0YCK5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Leucine-rich repeat-containing protein 51</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_39901</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YCK5"/>
    <dbReference type="OrthoDB" id="676979at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46545">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46545:SF1">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14580">
        <property type="entry name" value="LRR_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8020333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="229" mass="26764" checksum="158355C786943E8F" modified="2023-09-13" version="1">MTSFYHQLPRFPEDDAIKDGRRQRTKRVAVNFDSTFPYPAPPIDLSFRNLSSFEGKFPDLEEKPRHHPTKILCKTHKGKYESTALKFSNNLFENWIGFEQFVGKILHNPSALYWLDLSFNFLYTIDREILKFSQLKILYLHGNAIHSISEVDKLSNLDYLQSLTLHGNAVEEEKGYRLYVLKKLPKLRALDFSRVTRSETVSADLFSVSLMAANNRRRKEKRKTRSADM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YCQ3</accession>
    <name>A0A9Q0YCQ3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Conserved oligomeric Golgi complex subunit 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_44207</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000708">
        <property type="protein sequence ID" value="KAJ8018029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YCQ3"/>
    <dbReference type="OrthoDB" id="272987at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017119">
        <property type="term" value="C:Golgi transport complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010490">
        <property type="entry name" value="COG6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048369">
        <property type="entry name" value="COG6_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21506">
        <property type="entry name" value="COMPONENT OF OLIGOMERIC GOLGI COMPLEX 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21506:SF0">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20653">
        <property type="entry name" value="COG6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01087">
        <property type="entry name" value="COG6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Conserved Oligomeric Golgi complex subunit 6 C-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF20653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8018029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="280" mass="31023" checksum="117454B6EB003A28" modified="2023-09-13" version="1">MLAWLHQASASEKEHLISLLRMATAQPRDTEIQEVLGHITEGICRPFKVRVEQVIVAEPGPMVLYKLANLLKFYKYTIGQLLNKEAALTSTLSEMEEFAQKVFFNALNVLASKLVEKVELPPADLGPTATLSQTLSLLQEILASHDSSVVSMDLREQDFARILTSVLDPLMQMCSLSASRLNPADMATYMINCLHAMQTTLSLYEYTDQRLEMLQAQTDAHVDTLVNEQASFILSRTALLQIYSIALQHKPEEGPLASLPGLNTSAVKDGMVSQITFDGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YDA4</accession>
    <name>A0A9Q0YDA4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_42054</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000025">
        <property type="protein sequence ID" value="KAJ8019391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YDA4"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8019391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="153" mass="18047" checksum="EA139CDF91C00CDB" modified="2023-09-13" version="1">MELSEELCQQGWTLQWSCTFDWPYYFNTRTGQSQWHHLGQTIRIDQEQSRKRRHPRDNVQSRKHGGADPEASVEDDQPEQGTISPEICYTIEAPRIRRLKRFPTSINEYRVTFNESLLQRLVQQHKGPLSVLYNVVETLMNQIMDGFAPQDRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YF62</accession>
    <name>A0A9Q0YF62_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">SRCR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_39797</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YF62"/>
    <dbReference type="OrthoDB" id="6134459at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053231">
        <property type="entry name" value="GPCR_LN-TM7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036024">
        <property type="entry name" value="Somatomedin_B-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001212">
        <property type="entry name" value="Somatomedin_B_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45902">
        <property type="entry name" value="LATROPHILIN RECEPTOR-LIKE PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45902:SF1">
        <property type="entry name" value="LATROPHILIN RECEPTOR-LIKE PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01033">
        <property type="entry name" value="Somatomedin_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00530">
        <property type="entry name" value="SRCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00201">
        <property type="entry name" value="SO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90188">
        <property type="entry name" value="Somatomedin B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00524">
        <property type="entry name" value="SMB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50958">
        <property type="entry name" value="SMB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="164"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="192"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="4">
        <location>
            <begin position="36"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="4">
        <location>
            <begin position="222"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="SMB" evidence="5">
        <location>
            <begin position="341"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="4">
        <location>
            <begin position="672"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="128"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="249"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="295"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8020250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="710" mass="80175" checksum="785510CD9AEDA486" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YFY7</accession>
    <name>A0A9Q0YFY7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_39266</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YFY7"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8021928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="91" mass="10167" checksum="9CF20331D0A0AEA9" modified="2023-09-13" version="1">MNTTAQIVCFIFILSHEVLVIGTLVIIKIRTPEPRGENIQDSFPHSNPLAPEIVTYNGKNKTDGNVGEDDDLLDLIKDVGRKYGMTVSLYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YGE1</accession>
    <name>A0A9Q0YGE1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_37093</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YGE1"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8022252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="58" mass="6540" checksum="18DD99575E477FFD" modified="2023-09-13" version="1">MLFSKLEVLGLIPSWVMGRQVFIHEQPTVSFSRTNYPEGNSFLLELLLTESSPDALLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YHL3</accession>
    <name>A0A9Q0YHL3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase</fullName>
            <ecNumber evidence="4 14">4.2.1.134</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HOLleu_39888</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Nanhai2018</strain>
            <tissue evidence="15">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the third of the four reactions of the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acids/VLCFAs per cycle. This enzyme catalyzes the dehydration of the 3-hydroxyacyl-CoA intermediate into trans-2,3-enoyl-CoA, within each cycle of fatty acid elongation. Thereby, it participates to the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>a very-long-chain (3R)-3-hydroxyacyl-CoA = a very-long-chain (2E)-enoyl-CoA + H2O</text>
            <dbReference type="Rhea" id="RHEA:45812"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:83728"/>
            <dbReference type="ChEBI" id="CHEBI:85440"/>
            <dbReference type="EC" id="4.2.1.134"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Endoplasmic reticulum membrane</location>
            <topology evidence="14">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the very long-chain fatty acids dehydratase HACD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.134" evidence="4 14"/>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YHL3"/>
    <dbReference type="OrthoDB" id="46988at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102158">
        <property type="term" value="F:very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030497">
        <property type="term" value="P:fatty acid elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007482">
        <property type="entry name" value="Tyr_Pase-like_PTPLA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11035">
        <property type="entry name" value="VERY-LONG-CHAIN (3R)-3-HYDROXYACYL-COA DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11035:SF3">
        <property type="entry name" value="VERY-LONG-CHAIN (3R)-3-HYDROXYACYL-COA DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04387">
        <property type="entry name" value="PTPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11 14" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="7 14" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5 14" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9 14" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12 14" id="KW-0456">Lyase</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="84"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="114"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="149"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="194"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00036671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ8020322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="233" mass="26603" checksum="64F70A0941BC573D" modified="2023-09-13" version="1">MGSEAEEKAKKSETSGIATAYLVLYNVVLCAGWLSIGAISVRHYVQTKSIEGLYDNIELPLKVFQTAAVLEIFHCAVGIVRSSAFLTGMQVFSRVFIVWGVTHSVLEVQDEPSVALYVICWTITEIIRYAFYTFGLLNQLPYILTWLRYTLFIVLYPCGVIGELWTTYNALPHVQRTGLYSVSLPNDYNISFDFYKILIIWMFLYIPFFPRLFSHMLHQRRKVIGGQGGKKTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YHM0</accession>
    <name>A0A9Q0YHM0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="26" type="ORF">HOLleu_40358</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="26">Nanhai2018</strain>
            <tissue evidence="26">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Myofibril</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="26">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="5"/>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="504170at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031672">
        <property type="term" value="C:A band"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031674">
        <property type="term" value="C:I band"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008092">
        <property type="term" value="F:cytoskeletal protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060298">
        <property type="term" value="P:positive regulation of sarcomere organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045989">
        <property type="term" value="P:positive regulation of striated muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd05748">
        <property type="entry name" value="Ig_Titin_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000097">
        <property type="entry name" value="Bent, isoform F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000345">
        <property type="entry name" value="Muscle M-line assembly protein unc-89"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000425">
        <property type="entry name" value="Myosin light chain kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000080">
        <property type="entry name" value="Myosin light chain kinase, smooth muscle"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000107">
        <property type="entry name" value="Myosin, light chain kinase a"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000031">
        <property type="entry name" value="Myosin-binding protein C, slow type"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000032">
        <property type="entry name" value="palladin isoform X1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001238">
        <property type="entry name" value="Sallimus, isoform P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000160">
        <property type="entry name" value="Titin a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000003">
        <property type="entry name" value="Titin isoform E"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000012">
        <property type="entry name" value="titin isoform X1"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="2.60.40.10:FF:000127">
        <property type="entry name" value="titin isoform X1"/>
        <property type="match status" value="8"/>
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        <property type="entry name" value="twitchin isoform X4"/>
        <property type="match status" value="3"/>
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    <dbReference type="FunFam" id="2.60.40.10:FF:000051">
        <property type="entry name" value="Uncharacterized protein, isoform J"/>
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    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="42"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14340:SF9">
        <property type="entry name" value="FIBRONECTIN TYPE-III DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14340">
        <property type="entry name" value="MICROFIBRIL-ASSOCIATED GLYCOPROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="24"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00014">
        <property type="entry name" value="FNTYPEIII"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="24"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="24"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="22"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0106">Calcium</keyword>
    <keyword evidence="15" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="19" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
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        <location>
            <begin position="7"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="24">
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        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="24">
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    <feature type="domain" description="Ig-like" evidence="24">
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    <feature type="domain" description="Ig-like" evidence="24">
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    <feature type="domain" description="Ig-like" evidence="24">
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            <begin position="852"/>
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    <feature type="domain" description="Ig-like" evidence="24">
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            <begin position="988"/>
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    <feature type="domain" description="Ig-like" evidence="24">
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    <feature type="domain" description="Ig-like" evidence="24">
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            <begin position="1279"/>
            <end position="1361"/>
        </location>
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    <feature type="domain" description="Ig-like" evidence="24">
        <location>
            <begin position="1369"/>
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    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="25">
        <location>
            <begin position="1466"/>
            <end position="1561"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="25">
        <location>
            <begin position="1567"/>
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        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="25">
        <location>
            <begin position="1668"/>
            <end position="1762"/>
        </location>
    </feature>
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            <begin position="1766"/>
            <end position="1855"/>
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    </feature>
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        <location>
            <begin position="1863"/>
            <end position="1957"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="25">
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            <begin position="1963"/>
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            <begin position="2158"/>
            <end position="2253"/>
        </location>
    </feature>
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        <location>
            <begin position="2456"/>
            <end position="2550"/>
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    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="25">
        <location>
            <begin position="2556"/>
            <end position="2651"/>
        </location>
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            <begin position="2754"/>
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        <location>
            <begin position="2850"/>
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    </feature>
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        <location>
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            <begin position="3046"/>
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        <location>
            <begin position="3146"/>
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        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="25">
        <location>
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        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="25">
        <location>
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        </location>
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    <feature type="domain" description="Ig-like" evidence="24">
        <location>
            <begin position="3429"/>
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        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="25">
        <location>
            <begin position="3638"/>
            <end position="3732"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="25">
        <location>
            <begin position="3738"/>
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    </feature>
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        <location>
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    </feature>
    <feature type="domain" description="Ig-like" evidence="24">
        <location>
            <begin position="3932"/>
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    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="25">
        <location>
            <begin position="4026"/>
            <end position="4119"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="4171"/>
            <end position="4420"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="24">
        <location>
            <begin position="4487"/>
            <end position="4575"/>
        </location>
    </feature>
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        <location>
            <begin position="4604"/>
            <end position="4688"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="24">
        <location>
            <begin position="4874"/>
            <end position="4950"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="102"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="694"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="961"/>
            <end position="982"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="1549"/>
            <end position="1568"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="1687"/>
            <end position="1713"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="2632"/>
            <end position="2665"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="2733"/>
            <end position="2752"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="4739"/>
            <end position="4824"/>
        </location>
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        <location>
            <begin position="696"/>
            <end position="718"/>
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        <location>
            <begin position="805"/>
            <end position="818"/>
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        <location>
            <begin position="4739"/>
            <end position="4751"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
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            <begin position="4752"/>
            <end position="4785"/>
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        <location>
            <begin position="4792"/>
            <end position="4817"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="EMBL" id="KAJ8020699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="4982" mass="552031" checksum="E46163F95C31B2BB" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YIN2</accession>
    <name>A0A9Q0YIN2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Endonuclease/reverse transcript</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_35575</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YIN2"/>
    <dbReference type="OrthoDB" id="6154697at2759"/>
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    <dbReference type="PANTHER" id="PTHR33332">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8023228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="233" mass="26989" checksum="A257B693833EF806" modified="2023-09-13" version="1">MRFNSSKCYVMHISSGRSTTHFNYKLGGQTLQTVHSYPYLGIHLQDNLKWDTQVAHATTKASRVLGLIKRNLSNCTEQLKVTAYTSLVRPHLEYATPAWDPHLQKHCSQLERVQRNAARFVTHNYSKTEGTVTKLLHDLHWTSLQNRRSASRLTLLYKINQNLIDIPKENFLKPVTLQGLRRMNTQNFQQPACRINAYKYSFFPRTIKECNMLPEGTVNASSINSFKHNLHFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-06-18" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YL88</accession>
    <name>A0A9Q0YL88_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Adhesion G-protein coupled receptor G4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_37663</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
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            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Complement Module, domain 1"/>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
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        <property type="entry name" value="GCR1-cAMP_receptor"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017983">
        <property type="entry name" value="GPCR_2_secretin-like_CS"/>
    </dbReference>
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        <property type="entry name" value="GPS"/>
    </dbReference>
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        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47767">
        <property type="entry name" value="ADHESION G PROTEIN-COUPLED RECEPTOR G7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47767:SF1">
        <property type="entry name" value="ADHESION G PROTEIN-COUPLED RECEPTOR G7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02001">
        <property type="entry name" value="GCR1CAMPR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00303">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00650">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GAIN_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0768">Sushi</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040305934" evidence="10">
        <location>
            <begin position="24"/>
            <end position="1436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1145"/>
            <end position="1169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1181"/>
            <end position="1201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1213"/>
            <end position="1235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1247"/>
            <end position="1269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1289"/>
            <end position="1315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1336"/>
            <end position="1358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="1364"/>
            <end position="1387"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="13">
        <location>
            <begin position="298"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="GAIN-B" evidence="11">
        <location>
            <begin position="962"/>
            <end position="1138"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="12">
        <location>
            <begin position="1143"/>
            <end position="1389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="28"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="28"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="41"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="300"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ8022694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1436" mass="162072" checksum="E8FAC703F7B85F05" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YM42</accession>
    <name>A0A9Q0YM42_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Proline-rich AKT1 substrate 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_36556</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YM42"/>
    <dbReference type="OrthoDB" id="9992964at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032007">
        <property type="term" value="P:negative regulation of TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048011">
        <property type="term" value="P:neurotrophin TRK receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026682">
        <property type="entry name" value="AKT1S1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21844">
        <property type="entry name" value="AKT1 SUBSTRATE 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21844:SF2">
        <property type="entry name" value="PROLINE-RICH AKT1 SUBSTRATE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15798">
        <property type="entry name" value="PRAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="232"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="392"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="242"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="271"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="291"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="313"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="325"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8023966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="415" mass="46839" checksum="BF36589331ABF54A" modified="2023-09-13" version="1">MAQLSCECLNIVLHTKGNHHPVNVSSLQLPLHAASIPFFKGKVCEVQLDLGGISKEQECLVQERKISDWVTYKCSNCETWCYASHAVKGLSRVLANGNLLDSAKQDALRASDDYSPLFKIILQEKVFKLKEHSLVTPMTGNEDAVRNSAAQLQDLLTKYLTKEKMAVDERIKKFADEQRAMFASLQRRAYRDKNIMIGRILDAEEHNLEESLTEAMLDSSGDIDVETPAKNSSYIARDTDAKSNTSNQVVPSIPTQPRPAGRKVGLVKVQTVEDRQRRADQRVPLKSSAVDNDDTLFDLEEDFDKDDQKPFFESDEDEESSTDDNSFKAESPLDRRARPQYAASVPISMPVWPKDRYPLETNLEDDDDKLPMPEPDRMVESMRALSMSVQDGTEMFGDLPRPRLNTGDFSRKKGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YMM1</accession>
    <name>A0A9Q0YMM1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_35433</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8025270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YMM1"/>
    <dbReference type="OrthoDB" id="421226at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098855">
        <property type="term" value="C:HCN channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003254">
        <property type="term" value="P:regulation of membrane depolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.630">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013621">
        <property type="entry name" value="Ion_trans_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051413">
        <property type="entry name" value="K/Na_HCN_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689:SF5">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08412">
        <property type="entry name" value="Ion_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00103">
        <property type="entry name" value="CAMPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="118"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="145"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="259"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="342"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="10">
        <location>
            <begin position="440"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8025270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="593" mass="68987" checksum="A5F39784D45A9739" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YMW3</accession>
    <name>A0A9Q0YMW3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 13">D-3-phosphoglycerate dehydrogenase</fullName>
            <ecNumber evidence="4 13">1.1.1.95</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HOLleu_35505</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Nanhai2018</strain>
            <tissue evidence="17">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>(2R)-3-phosphoglycerate + NAD(+) = 3-phosphooxypyruvate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12641"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18110"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="EC" id="1.1.1.95"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 13">Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 1/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.95" evidence="4 13"/>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8025327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YMW3"/>
    <dbReference type="OrthoDB" id="1621027at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004617">
        <property type="term" value="F:phosphoglycerate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006564">
        <property type="term" value="P:L-serine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12173">
        <property type="entry name" value="PGDH_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000021">
        <property type="entry name" value="D-3-phosphoglycerate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000616">
        <property type="entry name" value="D-3-phosphoglycerate dehydrogenase 2 chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.90">
        <property type="entry name" value="D-3-phosphoglycerate dehydrogenase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029009">
        <property type="entry name" value="ASB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029753">
        <property type="entry name" value="D-isomer_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029752">
        <property type="entry name" value="D-isomer_DH_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006236">
        <property type="entry name" value="PGDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045626">
        <property type="entry name" value="PGDH_ASB_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01327">
        <property type="entry name" value="PGDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42938:SF22">
        <property type="entry name" value="D-3-PHOSPHOGLYCERATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42938">
        <property type="entry name" value="FORMATE DEHYDROGENASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19304">
        <property type="entry name" value="PGDH_inter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143548">
        <property type="entry name" value="Serine metabolism enzymes domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00065">
        <property type="entry name" value="D_2_HYDROXYACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00671">
        <property type="entry name" value="D_2_HYDROXYACID_DH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="7 13" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10 13" id="KW-0520">NAD</keyword>
    <keyword evidence="9 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 13" id="KW-0718">Serine biosynthesis</keyword>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase catalytic" evidence="14">
        <location>
            <begin position="8"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding" evidence="15">
        <location>
            <begin position="111"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="D-3-phosphoglycerate dehydrogenase ASB" evidence="16">
        <location>
            <begin position="329"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF19304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ8025327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="515" mass="54178" checksum="D1DFA91F03652606" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YNN7</accession>
    <name>A0A9Q0YNN7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_33446</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YNN7"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8025795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="67" mass="7369" checksum="8A35601B980ADFEB" modified="2023-09-13" version="1">MDPFTPKSRFEKRFAGNAAGPASIDLRLIQSGNRAIFVRRTIVAGHTIGFLAHRCLDFSLCCADCRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YNZ9</accession>
    <name>A0A9Q0YNZ9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_33623</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YNZ9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8025918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="55" mass="6047" checksum="08F092BF40EC20A9" modified="2023-09-13" version="1">MNFERQRKASLCEAKALVPGCLNSSEDEGDVGYSMMVKAVLVFVVPPTRHDYTVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YPY3</accession>
    <name>A0A9Q0YPY3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Myosin-11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_33218</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YPY3"/>
    <dbReference type="OrthoDB" id="10055605at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003774">
        <property type="term" value="F:cytoskeletal motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.360:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.360">
        <property type="entry name" value="Myosin S1 fragment, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008989">
        <property type="entry name" value="Myosin_S1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004009">
        <property type="entry name" value="SH3_Myosin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02736">
        <property type="entry name" value="Myosin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51844">
        <property type="entry name" value="SH3_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0505">Motor protein</keyword>
    <keyword evidence="5 8" id="KW-0518">Myosin</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myosin N-terminal SH3-like" evidence="10">
        <location>
            <begin position="26"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Myosin motor" evidence="9">
        <location>
            <begin position="80"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8025616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="116" mass="13111" checksum="42CB46AFE2FD8C9C" modified="2023-09-13" version="1">MDSESLKYLSVDKNRVNDPTAQAEWTAKKLVWIPDEKEGFVAGSIKSEKGDECTVELADSSKKKVVNKDDIQKMNPPKFTKVEDMADLTYLNEASVLYNLKDRYFSGLIYVIGKVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YQX5</accession>
    <name>A0A9Q0YQX5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Death domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_35047</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YQX5"/>
    <dbReference type="OrthoDB" id="10051937at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000488">
        <property type="entry name" value="Death_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00531">
        <property type="entry name" value="Death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="314"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="353"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="Death" evidence="2">
        <location>
            <begin position="24"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8024974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="400" mass="46273" checksum="27AD28E7A497BCF7" modified="2023-09-13" version="1">MKFHRLADFEVRRLPGFFKLSVNLSTLLDPEQPLGNDFRLVLEYLGFYPPEISFLASRYPRPTLEALKSWRGSTAELVEAFRLSEREDAINCLRVWLGQLAGYGRNVKDLTGPENKAVGQWSNGNSYRLLPSLASHFPSIEELLEQRYQPKETKRQTSDSFVIKEEECRVRRTSEYLCNTFTSFVTNCFVIPKDATWLTSSTVYVNNFARVYCFILILVNLVDCVCSSKHCPSYWLELIISLLPVMESFYVDKHIDSYKSAMKRLVECESEVCKLQNILERSVSRQTYDDLQSAKTLYETCLNEVQPKYTDLRLCVFSTLFVESFIATVLILRSLLFDTLQTFHERLGNGFTAIAYSLLIVDCFYNAMVVLLYYGDSTPRTVLRKDRFRHRQGVRGCVVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YRD1</accession>
    <name>A0A9Q0YRD1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Fibrinogen C domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_35439</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8025274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YRD1"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00087">
        <property type="entry name" value="FReD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.215.10:FF:000001">
        <property type="entry name" value="Tenascin isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050373">
        <property type="entry name" value="Fibrinogen_C-term_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143">
        <property type="entry name" value="FIBRINOGEN/TENASCIN/ANGIOPOEITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00186">
        <property type="entry name" value="FBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040132582" evidence="3">
        <location>
            <begin position="30"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="4">
        <location>
            <begin position="713"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="119"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="195"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="311"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="433"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="522"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="655"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="702"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="131"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="147"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="167"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="247"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="319"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="433"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="528"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="702"/>
            <end position="712"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8025274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="937" mass="103502" checksum="12619CB4550C352C" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YTD1</accession>
    <name>A0A9Q0YTD1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">glutamate carboxypeptidase II</fullName>
            <ecNumber evidence="16">3.4.17.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">HOLleu_31707</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="22">Nanhai2018</strain>
            <tissue evidence="22">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>Release of an unsubstituted, C-terminal glutamyl residue, typically from Ac-Asp-Glu or folylpoly-gamma-glutamates.</text>
            <dbReference type="EC" id="3.4.17.21"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M28 family. M28B subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.17.21" evidence="16"/>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YTD1"/>
    <dbReference type="OrthoDB" id="5841748at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08022">
        <property type="entry name" value="M28_PSMA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02121">
        <property type="entry name" value="PA_GCPII_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.930.40:FF:000001">
        <property type="entry name" value="N-acetylated-alpha-linked acidic dipeptidase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000009">
        <property type="entry name" value="N-acetylated-alpha-linked acidic dipeptidase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.30:FF:000045">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.40">
        <property type="entry name" value="Transferrin receptor-like, dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039373">
        <property type="entry name" value="Peptidase_M28B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007365">
        <property type="entry name" value="TFR-like_dimer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036757">
        <property type="entry name" value="TFR-like_dimer_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10404:SF77">
        <property type="entry name" value="GLUTAMATE CARBOXYPEPTIDASE 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10404">
        <property type="entry name" value="N-ACETYLATED-ALPHA-LINKED ACIDIC DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04253">
        <property type="entry name" value="TFR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47672">
        <property type="entry name" value="Transferrin receptor-like dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 22" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="PA" evidence="19">
        <location>
            <begin position="171"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M28" evidence="21">
        <location>
            <begin position="359"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="domain" description="Transferrin receptor-like dimerisation" evidence="20">
        <location>
            <begin position="632"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="45"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00052003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00066561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF04253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KAJ8026774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="766" mass="85602" checksum="362ED497096D7CF4" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YTZ8</accession>
    <name>A0A9Q0YTZ8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Protein RRP5-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_33969</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8026191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="412781at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05693">
        <property type="entry name" value="S1_Rrp5_repeat_hs1_sc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000065">
        <property type="entry name" value="Programmed cell death 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000103">
        <property type="entry name" value="protein RRP5 homolog"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055430">
        <property type="entry name" value="HAT_Syf1_CNRKL1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045209">
        <property type="entry name" value="Rrp5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057302">
        <property type="entry name" value="Rrp5_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048059">
        <property type="entry name" value="Rrp5_S1_rpt_hs1_sc1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23270">
        <property type="entry name" value="PROGRAMMED CELL DEATH PROTEIN 11 PRE-RRNA PROCESSING PROTEIN RRP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23270:SF10">
        <property type="entry name" value="PROTEIN RRP5 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23231">
        <property type="entry name" value="HAT_Syf1_CNRKL1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23459">
        <property type="entry name" value="S1_RRP5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="S1 motif" evidence="7">
        <location>
            <begin position="120"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="7">
        <location>
            <begin position="227"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="7">
        <location>
            <begin position="490"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="7">
        <location>
            <begin position="579"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="7">
        <location>
            <begin position="668"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="7">
        <location>
            <begin position="909"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="7">
        <location>
            <begin position="1112"/>
            <end position="1197"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="7">
        <location>
            <begin position="1306"/>
            <end position="1374"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="7">
        <location>
            <begin position="1399"/>
            <end position="1460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1064"/>
            <end position="1094"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1470"/>
            <end position="1810"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="75"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1068"/>
            <end position="1084"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1085"/>
            <end position="1094"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1470"/>
            <end position="1481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="1482"/>
            <end position="1501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1502"/>
            <end position="1527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="1528"/>
            <end position="1557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="1573"/>
            <end position="1597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1598"/>
            <end position="1641"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="1657"/>
            <end position="1669"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1670"/>
            <end position="1715"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1724"/>
            <end position="1734"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1741"/>
            <end position="1758"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1790"/>
            <end position="1810"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8026191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="2100" mass="237074" checksum="89A7EF6C82445A79" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BA29</accession>
    <name>A0A9Q1BA29_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_44652</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010001078">
        <property type="protein sequence ID" value="KAJ8017733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BA29"/>
    <dbReference type="OrthoDB" id="7700504at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46601:SF1">
        <property type="entry name" value="ADF-H DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46601">
        <property type="entry name" value="ULP_PROTEASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8017733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="535" mass="61006" checksum="99B6F3C56BB8E4A2" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BBE0</accession>
    <name>A0A9Q1BBE0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Mitochondrial inner membrane protein Mpv17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_40063</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the peroxisomal membrane protein PXMP2/4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BBE0"/>
    <dbReference type="OrthoDB" id="430207at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015267">
        <property type="term" value="F:channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901858">
        <property type="term" value="P:regulation of mitochondrial DNA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007248">
        <property type="entry name" value="Mpv17_PMP22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 2, PXMP2 MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266:SF17">
        <property type="entry name" value="PROTEIN MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04117">
        <property type="entry name" value="Mpv17_PMP22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="56"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="94"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8020465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="189" mass="21302" checksum="4469F39D29BC7F3E" modified="2023-09-13" version="1">MSRLWRAYLHLLNTHPIKVQGITTGALICVGDVLAQQFVEKKGLHGHDYARTIRQGIFGVVFVGPVMISWYRVLDRLYPGTGKLVPLYKVATDQVIFAPFIITCFMSFVAFGRGKTATEVKDQLSADFIPTLLTAYKIYPALQIFNFYIAPLNLRPVVVNVVSIFWNTYLSWISNNPTIQEVAVTKDTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BBX9</accession>
    <name>A0A9Q1BBX9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_41189</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8019549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BBX9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8019549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="82" mass="9302" checksum="AA930B9C7562DE20" modified="2023-09-13" version="1">MVFEIVDFHPSISQQLLVLALAWAKTCVPISELDFSAIMHVRKSVLFSNDTPWTKRTNANSFDVTMGSYDGVRYVNWVAFTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BC39</accession>
    <name>A0A9Q1BC39_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_42564</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000092">
        <property type="protein sequence ID" value="KAJ8019082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BC39"/>
    <dbReference type="OrthoDB" id="416454at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8019082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="87" mass="10395" checksum="767C37FA58DCA1C5" modified="2023-09-13" version="1">MLQVYKFLFERNPLSSCNYLKVQCNSRVRGHCKKLVKNFARLDIRKFSFSHRVVNEWNSLPEWAVNSTSVHCFKVNIDKFFHKCGRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BCG7</accession>
    <name>A0A9Q1BCG7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_38749</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BCG7"/>
    <dbReference type="OrthoDB" id="2113965at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8021515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="51" mass="5702" checksum="FB1500CD73568731" modified="2023-09-13" version="1">MRYGERKNPIIFVGGQRSSGVTGGQTLKTLLTRYLEVGSLDKFHTLYLDAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BD53</accession>
    <name>A0A9Q1BD53_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Galactosylceramide sulfotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_40383</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the galactose-3-O-sulfotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BD53"/>
    <dbReference type="OrthoDB" id="514299at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001733">
        <property type="term" value="F:galactosylceramide sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009247">
        <property type="term" value="P:glycolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009729">
        <property type="entry name" value="Gal-3-0_sulfotransfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14647">
        <property type="entry name" value="GALACTOSE-3-O-SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14647:SF85">
        <property type="entry name" value="GALACTOSYLCERAMIDE SULFOTRANSFERASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06990">
        <property type="entry name" value="Gal-3-0_sulfotr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040116429" evidence="11">
        <location>
            <begin position="20"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="419"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="427"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8020719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="443" mass="51948" checksum="B2FA6A449F88A7E3" modified="2023-09-13" version="1">MRKHWLLAVLLLCLTSTFSGFYLLHSSRSALINKFNMAVRLAVPYSTQKATTDFTTSLSTMTTLQSQKTLFINVSSGEMCNEVSNFVFVKTHKTGSTTLRSLTCRYGYFRNLSFLLGKQGVIGHLNQLNLVWTQNESNLLPPIGVSVEDHLNHRNYNISNIHVRYNGELIRKLMYRASDLKYFTILRNPTNQWLSQFQYFGQYRKAGLSLSSLPQTLIRYLKKLKERTGGFNNQLRDLGIHSSIAYNSSWLNETIPQIDKEYDLVLIMEHFDESLILLKKLLCWSFKDILYVKKRQQPNPLKINEATRKEIQKRNVGDEALYDYFLKVFWSKVEDYGPSFQKDLNQFRRMLNETWHMCVGKTVAEKSQDNYRYLETYVTDNSSEFCWALVNTNFQMDIEIVKRQGKTSDDRWPGLLKQSERKPVVKPQRRFPLRKGYKRKPIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BDF4</accession>
    <name>A0A9Q1BDF4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_40923</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MS4A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8021139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BDF4"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007237">
        <property type="entry name" value="CD20-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030417">
        <property type="entry name" value="MS4A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23320">
        <property type="entry name" value="MEMBRANE-SPANNING 4-DOMAINS SUBFAMILY A MS4A -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23320:SF130">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 212"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04103">
        <property type="entry name" value="CD20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="30"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="74"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="98"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="248"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8021139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="344" mass="38680" checksum="76E146522A043543" modified="2023-09-13" version="1">MNPTTNVMSQDSGRSIRPQPKRLRGTSERIFTAVVQLVCGIIAVACGLALVVLKTPYNDNYYGYQITRIGKTAFGIWGGLIFIATAIPYFFGRHRKSMVGLYFGFCLASAVIAVVGLGLSITAINKIVYTTSGTLICNDEALGIQRDQKYRCCFDEYRYRYDCRPSYNHRFSWYDYHLPATYGYQRLNYNSLECESFRSYLRDSYKQCVQEIDLIKTIHHNALQRFTNFTSFVQLVLNTVKRVNMPHLAFYGIMVAVVSVELFASVFGACAALSALCNCCVGGKPTQYYPCSFNQLMVNDSLNNTVIPPQVTTQQRGPTVISTTQVPQTFNPLQVPPQTVPLGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BDV3</accession>
    <name>A0A9Q1BDV3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_38076</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8023015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BDV3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="701"/>
            <end position="731"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8023015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="804" mass="91563" checksum="5434B04307F95E41" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BGE1</accession>
    <name>A0A9Q1BGE1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Synaptotagmin-like protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_31617</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BGE1"/>
    <dbReference type="OrthoDB" id="195679at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070382">
        <property type="term" value="C:exocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042043">
        <property type="term" value="F:neurexin family protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08521">
        <property type="entry name" value="C2A_SLP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04020">
        <property type="entry name" value="C2B_SLP_1-2-3-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000006">
        <property type="entry name" value="Synaptotagmin-like 5, isoform CRA_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043567">
        <property type="entry name" value="SYTL1-5_C2B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45716">
        <property type="entry name" value="BITESIZE, ISOFORM I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45716:SF2">
        <property type="entry name" value="BITESIZE, ISOFORM I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="C2" evidence="5">
        <location>
            <begin position="295"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="5">
        <location>
            <begin position="447"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="36"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="226"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="43"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="226"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8026706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="615" mass="69124" checksum="D535AC0D610E6A2A" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BH30</accession>
    <name>A0A9Q1BH30_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 13">NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HOLleu_34973</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Nanhai2018</strain>
            <tissue evidence="15">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 13">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 13">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the complex I NDUFA10 subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BH30"/>
    <dbReference type="OrthoDB" id="17400at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006120">
        <property type="term" value="P:mitochondrial electron transport, NADH to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050566">
        <property type="entry name" value="Deoxyribonucleoside_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031314">
        <property type="entry name" value="DNK_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015828">
        <property type="entry name" value="NDUFA10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10513">
        <property type="entry name" value="DEOXYNUCLEOSIDE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10513:SF15">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 10, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01712">
        <property type="entry name" value="dNK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000543">
        <property type="entry name" value="NADH_UQ_42KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 13" id="KW-0249">Electron transport</keyword>
    <keyword evidence="9 13" id="KW-0274">FAD</keyword>
    <keyword evidence="7 13" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="12 13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="6 13" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Deoxynucleoside kinase" evidence="14">
        <location>
            <begin position="60"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR000543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ8024919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="360" mass="42375" checksum="BD057224BB01761C" modified="2023-09-13" version="1">MAFHISKIAKQTFQSGKHLCKVGQLLRNHTQVRTVMTETPLTFVFGYKFMHKFDENSKIIVLDGNLAVGKTTMAADLADKLGMKYFPECHVHYMDEKVMGSGQKCDSKFSGDVSLDRFYEDPLAKDGHTFRFQMVMYALRFLQYRDVVLHLLETGQGVVMERSVYSDFVFLEAIMDMGWIRRECYDYYNQIKGLSLYRMKHPHAVVYLDVPPEFAHQRFVERGKGYEKNVPLEYFQALDKAYKQKYLPEMKAAGVEVLHYDWKSFGDVDRIADDIGYLKCEDSVWNTVDNAILDRHYRFLANEWKVLSCMNIPRFVPEITYGGQQFEDLIEEYKLKYHKRYAPGFNEGDYKSKWEIMFKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BHM9</accession>
    <name>A0A9Q1BHM9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Reverse transcriptase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_33272</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome 17"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09274">
        <property type="entry name" value="RNase_HI_RT_Ty3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22249">
        <property type="entry name" value="UDM1_RNF168_RNF169-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Polyprotein of retroviral origin, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 17.6-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000032">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 297-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon gypsy-like Protein"/>
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    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054465">
        <property type="entry name" value="Integrase_p58-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041373">
        <property type="entry name" value="RT_RNaseH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22938">
        <property type="entry name" value="Integrase_p58_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17917">
        <property type="entry name" value="RT_RNaseH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="9">
        <location>
            <begin position="868"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase" evidence="8">
        <location>
            <begin position="1282"/>
            <end position="1459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="95"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="337"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="601"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="701"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="337"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="352"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="376"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="601"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="645"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="670"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="701"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="722"/>
            <end position="741"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8025654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1674" mass="191392" checksum="C5F58DDA8EEC75FD" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BJU0</accession>
    <name>A0A9Q1BJU0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_30040</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8027941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BJU0"/>
    <dbReference type="OrthoDB" id="5987257at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31751">
        <property type="entry name" value="SI:CH211-108C17.2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31751:SF44">
        <property type="entry name" value="SI:CH211-211K8.4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="195"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="196"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8027941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="235" mass="26537" checksum="41CE85EBE3C7D179" modified="2023-09-13" version="1">MDWTQSIINHLYWCASSTPTGDPQMMLEKFLSIENHIQNKHSGHPGELFVECAHEPIPAEAERTKAWLEPGTEPCEKLSAVLNQTHLKNDVKKMSPKEQTSSIEAFHSLILHFAPKQLVFSYEGMTCRTLLAALHYNENSGRCQARSSEGKTAFKLDFPRAKCGGYTLRKRLVKATFGYVDDLLSEVHRLLERGDQTQLDPESRISSPPPTLSSSHEGPTLEDAIRKHYTRFSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BKF8</accession>
    <name>A0A9Q1BKF8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">15-oxoprostaglandin 13-reductase</fullName>
            <ecNumber evidence="2">1.3.1.48</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">15-oxoprostaglandin 13-reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_30277</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>13,14-dihydro-15-oxo-PGF2alpha + NADP(+) = 15-oxoprostaglandin F2alpha + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50588"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:133374"/>
            <dbReference type="ChEBI" id="CHEBI:133409"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="6">
            <dbReference type="Rhea" id="RHEA:50590"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>13,14-dihydro-15-oxo-prostaglandin E1 + NADP(+) = 15-oxoprostaglandin E1 + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50584"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57401"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:133408"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="7">
            <dbReference type="Rhea" id="RHEA:50586"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>13,14-dihydro-15-oxo-prostaglandin F1alpha + NADP(+) = 15-oxoprostaglandin F1alpha + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50592"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:79072"/>
            <dbReference type="ChEBI" id="CHEBI:133411"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="5">
            <dbReference type="Rhea" id="RHEA:50594"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NADP-dependent oxidoreductase L4BD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.48" evidence="2"/>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BKF8"/>
    <dbReference type="OrthoDB" id="809632at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047522">
        <property type="term" value="F:15-oxoprostaglandin 13-reductase [NAD(P)+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006693">
        <property type="term" value="P:prostaglandin metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000121">
        <property type="entry name" value="Prostaglandin reductase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041694">
        <property type="entry name" value="ADH_N_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045010">
        <property type="entry name" value="MDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43205">
        <property type="entry name" value="PROSTAGLANDIN REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43205:SF5">
        <property type="entry name" value="PROSTAGLANDIN REDUCTASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16884">
        <property type="entry name" value="ADH_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Oxidoreductase N-terminal" evidence="9">
        <location>
            <begin position="7"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="Alcohol dehydrogenase-like C-terminal" evidence="8">
        <location>
            <begin position="173"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00033119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8028129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="353" mass="39045" checksum="DAD2273BE6C9C082" modified="2023-09-13" version="1">MAKNHCVVLDSRPGEDNVPTVDNFCLKEYPVPDIEDGQILVKTLCLSVDPYMRSRFNQHTGTDYMSPWKLDCPIDGGGVGAVVESKSRHFCKGEIVESIHWPWQEYAMFEVEGFNSLILNKVESELVKSHVSLMLGVLGVPGLTALLGISEKGHVTEGANQTFVVSAAAGACGSLAGQIARVEGCTNVVGICGTEEKCKFLTDDLKFDAAINYKDDDVDSKLKEYCPGGIDIYFDNVGGQISNSVIKQMNKDSHIILCGQISMYNTSQPYPPPLEEDIENIRQERNITRDRFLVLNYYDQFPKALAQLKKWYEEGKLKYRETISEGLEKAPEAFVSMMSGGNIGKQIVHVSGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BLF4</accession>
    <name>A0A9Q1BLF4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_31115</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BLF4"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8028780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="78" mass="9031" checksum="0E875C880D94126C" modified="2023-09-13" version="1">MKSPETIRHLRQAHWITRPQPHRELFVICANIVTDDPDGIVARLRHTINAPSTEEFARKVADNIDDKEDDTKRNNNTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BNN8</accession>
    <name>A0A9Q1BNN8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Protein lin-54-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_26432</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the lin-54 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="8300196at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005172">
        <property type="entry name" value="CRC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033467">
        <property type="entry name" value="Tesmin/TSO1-like_CXC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46704:SF9">
        <property type="entry name" value="BHLH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46704">
        <property type="entry name" value="CXC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03638">
        <property type="entry name" value="TCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01114">
        <property type="entry name" value="CXC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51634">
        <property type="entry name" value="CRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRC" evidence="5">
        <location>
            <begin position="1283"/>
            <end position="1318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="60"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="714"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="718"/>
            <end position="736"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8030123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1335" mass="149961" checksum="99F6F06DC0BB75C9" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BPJ4</accession>
    <name>A0A9Q1BPJ4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Monocarboxylate transporter 9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_26880</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BPJ4"/>
    <dbReference type="OrthoDB" id="6499973at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008028">
        <property type="term" value="F:monocarboxylic acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17352">
        <property type="entry name" value="MFS_MCT_SLC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF284">
        <property type="entry name" value="EG:103B4.3 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="72"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="145"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="176"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="203"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="368"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="402"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="430"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="455"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="490"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="525"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="4">
        <location>
            <begin position="33"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="557"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="573"/>
            <end position="584"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8030446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="614" mass="66513" checksum="12F8A31658860257" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BRW1</accession>
    <name>A0A9Q1BRW1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">G-protein coupled receptor Mth2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_24733</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BRW1"/>
    <dbReference type="OrthoDB" id="6134459at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="GPCR_2-like_7TM"/>
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        <property type="entry name" value="GPCR_2_secretin-like"/>
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    <dbReference type="InterPro" id="IPR053231">
        <property type="entry name" value="GPCR_LN-TM7"/>
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    <dbReference type="PANTHER" id="PTHR45902">
        <property type="entry name" value="LATROPHILIN RECEPTOR-LIKE PROTEIN A"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR45902:SF1">
        <property type="entry name" value="LATROPHILIN RECEPTOR-LIKE PROTEIN A"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
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    </dbReference>
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            <begin position="388"/>
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    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="496"/>
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    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="584"/>
            <end position="605"/>
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    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="611"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="6">
        <location>
            <begin position="386"/>
            <end position="633"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8031520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="664" mass="74703" checksum="BF3AE5D600BD98D5" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BS46</accession>
    <name>A0A9Q1BS46_HOLLE</name>
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        </submittedName>
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    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome 12"/>
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    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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            <begin position="1559"/>
            <end position="1581"/>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1686" mass="177672" checksum="C93F305A3643D55A" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
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    <name>A0A9Q1BUL2_HOLLE</name>
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        </submittedName>
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        <name type="common">Black long sea cucumber</name>
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        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
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            <taxon>Aspidochirotacea</taxon>
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        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="FIBRINOGEN/TENASCIN/ANGIOPOEITIN"/>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="284" mass="32738" checksum="A2264420C701108B" modified="2023-09-13" version="1">MYLILGRSCIYCVLLISIGTEICLTSQTHGQPTDEGVIPRQKRDINDANGSSYFYFQQPEYPRDCQEVYDECQNTLDGVYLIKPDGYSEPFEVFCNNTIDSGGWTVFQRRMDGGVDFYRDWVDYKTGFGFLHREFWLGNEKIAFITNQKDYEMRLDMVDSSGSTSYAYYNLFRISDEGTKYSLVEVGSYNGTIGLLEGHDYLATHRGHPFSTRDRDNDNRDTGEPNCAVHHHGAWWYTNCYSSNLNGDYQSSADTGLCLYDSTNSNQCNFRFTEMKIRPTQTNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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    <name>A0A9Q1BXB9_HOLLE</name>
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        <submittedName>
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        </submittedName>
    </protein>
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    </gene>
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        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
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            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome 10"/>
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    <dbReference type="PANTHER" id="PTHR46534:SF1">
        <property type="entry name" value="IGGFC-BINDING PROTEIN N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040148901" evidence="3">
        <location>
            <begin position="22"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="595"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="domain" description="HYR" evidence="4">
        <location>
            <begin position="424"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="domain" description="HYR" evidence="4">
        <location>
            <begin position="509"/>
            <end position="587"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8034601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="626" mass="69393" checksum="684B6F088435EF8D" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BXP5</accession>
    <name>A0A9Q1BXP5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Hemicentin-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_21609</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BXP5"/>
    <dbReference type="OrthoDB" id="5985519at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005911">
        <property type="term" value="C:cell-cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050839">
        <property type="term" value="F:cell adhesion molecule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000032">
        <property type="entry name" value="palladin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051275">
        <property type="entry name" value="Cell_adhesion_signaling"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11640:SF164">
        <property type="entry name" value="MAM DOMAIN-CONTAINING GLYCOSYLPHOSPHATIDYLINOSITOL ANCHOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11640">
        <property type="entry name" value="NEPHRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="3 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="720"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="9">
        <location>
            <begin position="123"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="9">
        <location>
            <begin position="213"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="9">
        <location>
            <begin position="320"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="9">
        <location>
            <begin position="425"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="9">
        <location>
            <begin position="514"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="10">
        <location>
            <begin position="603"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="690"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="690"/>
            <end position="699"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8034675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="802" mass="88385" checksum="1D9C79FFB35B07FD" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BYZ8</accession>
    <name>A0A9Q1BYZ8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_22128</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BYZ8"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8035046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="146" mass="15362" checksum="2458FAD8375A0A9F" modified="2023-09-13" version="1">MKVDDVWVFALALECSESTVTVEGSGDKSAGVEMKVDDVWVFALALECSESTVTVEGSGDKSAGVEMKVDDVWVFALALECSESTVTVEGSGDKSAGVEMKVDDVWVFALELDCSESTVTVEGSGDKSAGVEMKVDDVVISFSSSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BZZ5</accession>
    <name>A0A9Q1BZZ5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Molybdenum cofactor sulfurase</fullName>
            <shortName evidence="4">MCS</shortName>
            <shortName evidence="4">MOS</shortName>
            <shortName evidence="4">MoCo sulfurase</shortName>
            <ecNumber evidence="4">2.8.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Molybdenum cofactor sulfurtransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_19869</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Sulfurates the molybdenum cofactor. Sulfation of molybdenum is essential for xanthine dehydrogenase (XDH) and aldehyde oxidase (ADO) enzymes in which molybdenum cofactor is liganded by 1 oxygen and 1 sulfur atom in active form.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>Mo-molybdopterin + L-cysteine + AH2 = thio-Mo-molybdopterin + L-alanine + A + H2O</text>
            <dbReference type="Rhea" id="RHEA:42636"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:71302"/>
            <dbReference type="ChEBI" id="CHEBI:82685"/>
            <dbReference type="EC" id="2.8.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. MOCOS subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.9" evidence="4"/>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BZZ5"/>
    <dbReference type="OrthoDB" id="420046at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008265">
        <property type="term" value="F:molybdenum cofactor sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030151">
        <property type="term" value="F:molybdenum ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03050">
        <property type="entry name" value="MOCOS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005302">
        <property type="entry name" value="MoCF_Sase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028886">
        <property type="entry name" value="MoCo_sulfurase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005303">
        <property type="entry name" value="MOCOS_middle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011037">
        <property type="entry name" value="Pyrv_Knase-like_insert_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14237:SF80">
        <property type="entry name" value="MOLYBDENUM COFACTOR SULFURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14237">
        <property type="entry name" value="MOLYBDOPTERIN COFACTOR SULFURASE MOSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03473">
        <property type="entry name" value="MOSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03476">
        <property type="entry name" value="MOSC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141673">
        <property type="entry name" value="MOSC N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50800">
        <property type="entry name" value="PK beta-barrel domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51340">
        <property type="entry name" value="MOSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="2 4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MOSC" evidence="5">
        <location>
            <begin position="635"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="409"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="4">
        <location>
            <position position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8036017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="789" mass="88157" checksum="8E73337BB817CA27" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C324</accession>
    <name>A0A9Q1C324_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_18300</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C324"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8037477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="122" mass="13162" checksum="5F7CF74082948BFA" modified="2023-09-13" version="1">MPHIERKNPIIFGGDQRSPGVTGGQTLKTLLTQYLEVKRKAESSGATKKGGMRAFSTVMAFPPLSPSPPQYKLPGSPMLVCTSLGGVAMSHLQVAKISALNNKNFFVCNKLKDLPKIVTFCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C3C6</accession>
    <name>A0A9Q1C3C6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Tuberin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_18229</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalytic component of the TSC-TBC complex, a multiprotein complex that acts as a negative regulator of the canonical mTORC1 complex, an evolutionarily conserved central nutrient sensor that stimulates anabolic reactions and macromolecule biosynthesis to promote cellular biomass generation and growth. Within the TSC-TBC complex, TSC2 acts as a GTPase-activating protein (GAP) for the small GTPase RHEB, a direct activator of the protein kinase activity of mTORC1. In absence of nutrients, the TSC-TBC complex inhibits mTORC1, thereby preventing phosphorylation of ribosomal protein S6 kinase (RPS6KB1 and RPS6KB2) and EIF4EBP1 (4E-BP1) by the mTORC1 signaling. The TSC-TBC complex is inactivated in response to nutrients, relieving inhibition of mTORC1. Involved in microtubule-mediated protein transport via its ability to regulate mTORC1 signaling. Also stimulates the intrinsic GTPase activity of the Ras-related proteins RAP1A and RAB5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Lysosome membrane</location>
            <topology evidence="8">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="5797019at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033596">
        <property type="term" value="C:TSC1-TSC2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046627">
        <property type="term" value="P:negative regulation of insulin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051898">
        <property type="term" value="P:negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032007">
        <property type="term" value="P:negative regulation of TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030178">
        <property type="term" value="P:negative regulation of Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016241">
        <property type="term" value="P:regulation of macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051056">
        <property type="term" value="P:regulation of small GTPase mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11210:FF:000004">
        <property type="entry name" value="Tuberin isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11210">
        <property type="entry name" value="Rap/Ran-GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035974">
        <property type="entry name" value="Rap/Ran-GAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000331">
        <property type="entry name" value="Rap/Ran_GAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003913">
        <property type="entry name" value="Tuberin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018515">
        <property type="entry name" value="Tuberin-type_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027107">
        <property type="entry name" value="Tuberin/Ral-act_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024584">
        <property type="entry name" value="Tuberin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10063">
        <property type="entry name" value="TUBERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10063:SF0">
        <property type="entry name" value="TUBERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11864">
        <property type="entry name" value="DUF3384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02145">
        <property type="entry name" value="Rap_GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03542">
        <property type="entry name" value="Tuberin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01431">
        <property type="entry name" value="TUBERIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111347">
        <property type="entry name" value="Rap/Ran-GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50085">
        <property type="entry name" value="RAPGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="7" id="KW-0458">Lysosome</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0832">Ubl conjugation</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="861"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="886"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="domain" description="Rap-GAP" evidence="13">
        <location>
            <begin position="1598"/>
            <end position="1827"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="934"/>
            <end position="976"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1060"/>
            <end position="1148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1221"/>
            <end position="1262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1424"/>
            <end position="1447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1483"/>
            <end position="1531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="934"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="962"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1118"/>
            <end position="1136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="1222"/>
            <end position="1239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1427"/>
            <end position="1447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00054764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00070662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ8037419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1863" mass="209500" checksum="682E7ECB940391AA" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C3K8</accession>
    <name>A0A9Q1C3K8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Solute carrier family 23 member 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_18937</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C3K8"/>
    <dbReference type="OrthoDB" id="1641903at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006043">
        <property type="entry name" value="NCS2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11119">
        <property type="entry name" value="XANTHINE-URACIL / VITAMIN C PERMEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00860">
        <property type="entry name" value="Xan_ur_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="9"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="82"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="149"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="230"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="258"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="289"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8037982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="422" mass="46602" checksum="8F4A6BFB197291F5" modified="2023-09-13" version="1">MGVTGFISFMLRFVGPLSVAPVLMLLGISLTQLSWESCHSHWGVSVFTAGMIILFTQFLSKKKIPVIGFSREKGFHVVRTRFLLKYSMILATMSGWFLCWILTMAGAFSDDPSDRTYRARTDIANEVFNETPWIRFPYPGQVVWPRPSIAAFFGMFSAVIASIIESIGDYNACARICQIPSPPRHAVNRGIATEGLGGLLSCLWGTGTGTGSYSVNIVIIGITKVASRRVIQMAAIMMVVVACFQKLCSFFATMPDPVIGGTMIPTVGMLISVGISLLQKIDINSSRNLFVLGLSLFLGIMLPEYMQNNPGIIHTGLSTFDKCATILLETGIFVGFITGLVFDNVIPDLCFCCPGTLMERGLLHNQQSKETAEHRPGDPDDKQSCYNLPVGMNSIRRWRFCRFVPISPTFRGFPRLRRASSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C439</accession>
    <name>A0A9Q1C439_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Tetratricopeptide repeat protein 30</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_18695</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Required for polyglutamylation of axonemal tubulin. Plays a role in anterograde intraflagellar transport (IFT), the process by which cilia precursors are transported from the base of the cilium to the site of their incorporation at the tip.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cell projection</location>
            <location evidence="1 10">Cilium</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the TTC30/dfy-1/fleer family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C439"/>
    <dbReference type="OrthoDB" id="10249577at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005879">
        <property type="term" value="C:axonemal microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030992">
        <property type="term" value="C:intraciliary transport particle B"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120170">
        <property type="term" value="F:intraciliary transport particle B binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042073">
        <property type="term" value="P:intraciliary transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000226">
        <property type="entry name" value="Tetratricopeptide repeat protein 30A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000211">
        <property type="entry name" value="tetratricopeptide repeat protein 30B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039941">
        <property type="entry name" value="TT30"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20931">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20931:SF0">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13174">
        <property type="entry name" value="TPR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7 10" id="KW-0969">Cilium</keyword>
    <keyword evidence="4 10" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 9 10" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="42"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU367070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8037790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="657" mass="76177" checksum="B263E79B459C3394" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CCF6</accession>
    <name>A0A9Q1CCF6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">NFX1-type zinc finger-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_13114</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8042125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2423195at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031380">
        <property type="term" value="C:nuclear RNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031048">
        <property type="term" value="P:regulatory ncRNA-mediated heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17936">
        <property type="entry name" value="EEXXEc_NFX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000742">
        <property type="entry name" value="NFX1-type zinc finger-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045055">
        <property type="entry name" value="DNA2/NAM7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000967">
        <property type="entry name" value="Znf_NFX1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057373">
        <property type="entry name" value="ZNFX1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887:SF341">
        <property type="entry name" value="NFX1-TYPE ZINC FINGER-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25396">
        <property type="entry name" value="ZNFX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00438">
        <property type="entry name" value="ZnF_NFX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040121263" evidence="5">
        <location>
            <begin position="20"/>
            <end position="1322"/>
        </location>
    </feature>
    <feature type="domain" description="NF-X1-type" evidence="6">
        <location>
            <begin position="1041"/>
            <end position="1063"/>
        </location>
    </feature>
    <feature type="domain" description="NF-X1-type" evidence="6">
        <location>
            <begin position="1205"/>
            <end position="1222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8042125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1322" mass="153082" checksum="69CB914CAC96DBBE" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CCV2</accession>
    <name>A0A9Q1CCV2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_13360</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8042335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CCV2"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8042335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="60" mass="7102" checksum="351149FA80752EFC" modified="2023-09-13" version="1">MPCRSILHIYKWLLCPQTNMGSYPQGHEECLTLHVLYKSCIIWQRCCHPSSLMDKEILPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CDH2</accession>
    <name>A0A9Q1CDH2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF6570 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_10366</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CDH2"/>
    <dbReference type="OrthoDB" id="6141723at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046700">
        <property type="entry name" value="DUF6570"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20209">
        <property type="entry name" value="DUF6570"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6570" evidence="2">
        <location>
            <begin position="9"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="127"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8043331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="190" mass="21661" checksum="82F8AAF4A5318916" modified="2023-09-13" version="1">MQIRELPRRRQLNLKGNVVNVPVVSSTCSLLPRSLDETMTIPVKFKRKLSFKHSVQSENIRPKKVIDAAQWLVANSQLYKNENVVIHQHWSQTIQQMDDDWHEFIEQPGNQYDKHDNAVSPVGATLSIQTNDDNNPDADDWSEAQNEEMQPTGTLDTMLIPEFHEQSHLAYCIAPGEGNRPTGLFQDSLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CDU2</accession>
    <name>A0A9Q1CDU2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Somatomedin-B and thrombospondin type-1 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_09772</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8042893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CDU2"/>
    <dbReference type="OrthoDB" id="98591at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000134">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039942">
        <property type="entry name" value="SBSPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056801">
        <property type="entry name" value="SBSPON_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036024">
        <property type="entry name" value="Somatomedin_B-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001212">
        <property type="entry name" value="Somatomedin_B_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044004">
        <property type="entry name" value="TSP1_spondin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20920">
        <property type="entry name" value="RPE-SPONDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20920:SF5">
        <property type="entry name" value="SMB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25031">
        <property type="entry name" value="SBSPON_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19028">
        <property type="entry name" value="TSP1_spondin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90188">
        <property type="entry name" value="Somatomedin B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00524">
        <property type="entry name" value="SMB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50958">
        <property type="entry name" value="SMB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040275545" evidence="4">
        <location>
            <begin position="25"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="SMB" evidence="5">
        <location>
            <begin position="34"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8042893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="269" mass="30002" checksum="46C0D9ACCB820A09" modified="2023-09-13" version="1">MERSIGILCLVVFIIASFIHLSDASCADGGPDGGPLCCLGKDNDCTAEGYQIFARSYGTCYCDTACVNTRDCCTDFDQACPAVDCEVSPWSLWSGCSERCGVGYSERRRFVLREPQNAGAKCPKLQERRGCKGYRGCELEDIPGNNVAMILPYKYSLSRAGPQWEHPLARAPRFDTYCVYFKFRRFHPTCETPGIENWTDSLKKGSRACVECNRPAYGTSGVCNGEGINKKKTRWSAVDIQGCRGVWQRIDIIKDCTCGEDEENRFVFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CEF7</accession>
    <name>A0A9Q1CEF7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Splicing factor ESS-2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_11281</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ESS2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CEF7"/>
    <dbReference type="OrthoDB" id="19679at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019148">
        <property type="entry name" value="Nuclear_protein_DGCR14_ESS-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12940">
        <property type="entry name" value="ES-2 PROTEIN - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12940:SF0">
        <property type="entry name" value="SPLICING FACTOR ESS-2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09751">
        <property type="entry name" value="Es2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="83"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="385"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="84"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8043953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="504" mass="56356" checksum="CE5C5EF4BB495B93" modified="2023-09-13" version="1">MEVARVGEKALAALEENKELAVPPRQKRTKKVLDEEKYVEDLEKIIQRDFFPDLPKLKAQKEYLEAKQKNDLVKMRELALKFATTEQQSNTSTPGQASEVATPSTFETPDLHSQPASPHKSSQRKDGGDDDEEETFNPEPGRSSKQEKSDTSLSLDKYLSKHTSEDNASFVEIMETARQKSRQKHAWLYEAVEKQAIEQEEKLQLKGPETLAIKGPSDSKVKTWTYTPKNMLMYVPDGIEYNATEKMDEVKKEREIKHSNTRFDGTPFAVGTHKNTMANAVAQQASKQQGKIGPDGRELVVNQGPSVNGYGFVATPSPAPGVNESPLMTWGEIESTPFRIESGNTPRASPGPQFKMPAVPRRDRLGLSLVEQASKQHRAKKEAALKRVTQNLSSPSPKRFGSARSMERLHSLSPAAQRLVNKSMSRVRTDKSLRASYTPSPRSLPGDKTPILRTTPGRTPDKTPKKTPRQTPKTTPKRTESVSLTDNLLDLPRRNTRPKASDFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CFY5</accession>
    <name>A0A9Q1CFY5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Malectin-A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_11283</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the malectin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CFY5"/>
    <dbReference type="OrthoDB" id="10013439at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.430">
        <property type="entry name" value="Galactose-binding lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021720">
        <property type="entry name" value="Malectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039155">
        <property type="entry name" value="MLEC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13460:SF0">
        <property type="entry name" value="MALECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11721">
        <property type="entry name" value="Malectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040175536" evidence="12">
        <location>
            <begin position="28"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="251"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="Malectin" evidence="13">
        <location>
            <begin position="31"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="196"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="210"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF11721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ8043955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="272" mass="30356" checksum="3E5373DB499B0D4C" modified="2023-09-13" version="1">MISTDLQTWFVCVVTVLIIAHSNFSRGSPQVIFAVNCGGQEHIDVHGVRYQADFNRVGTPSDYGKNLNIQRVPLQDAILYQTERYHVDTFSYTIPIPEEGDFVLILKFSEVWFAASSEKVFDVMLNDEHTVVSDLDIYEKVGLGTAHDEIIPFSVRKGKLKVSSETSEFKGDLVVSFVKGDNDNPKINAIVVMKGSPEDVPTLPPLPWNTDDDIEGDDEDDDAEEVTEKKQIKHKSGPKTVDPYASDDSSLIFPILISIAVFIPTLFCLCRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CH32</accession>
    <name>A0A9Q1CH32_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Gamma-aminobutyric acid type B receptor subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HOLleu_07676</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Nanhai2018</strain>
            <tissue evidence="15">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CH32"/>
    <dbReference type="OrthoDB" id="2150267at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038039">
        <property type="term" value="C:G protein-coupled receptor heterodimeric complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004965">
        <property type="term" value="F:G protein-coupled GABA receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007214">
        <property type="term" value="P:gamma-aminobutyric acid signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15047">
        <property type="entry name" value="7tmC_GABA-B-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06366">
        <property type="entry name" value="PBP1_GABAb_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000379">
        <property type="entry name" value="Gamma-aminobutyric acid B receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000063">
        <property type="entry name" value="Gamma-aminobutyric acid type B receptor subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002455">
        <property type="entry name" value="GPCR3_GABA-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000337">
        <property type="entry name" value="GPCR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10519:SF20">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 156-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10519">
        <property type="entry name" value="GABA-B RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01177">
        <property type="entry name" value="GABAB1RECPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01176">
        <property type="entry name" value="GABABRECEPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00248">
        <property type="entry name" value="GPCRMGR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 15" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="399"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="443"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="466"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="528"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="558"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="586"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="13">
        <location>
            <begin position="318"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="14">
        <location>
            <begin position="466"/>
            <end position="630"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00073785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ8044815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="668" mass="75484" checksum="97F129549B701585" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CHG7</accession>
    <name>A0A9Q1CHG7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">ATP-binding cassette sub-family B member 10, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">ABC-mitochondrial erythroid protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">ATP-binding cassette transporter 10</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">HOLleu_08359</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="24">Nanhai2018</strain>
            <tissue evidence="24">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">ATP-dependent transporter located in the mitochondrial inner membrane that catalyzes the export of biliverdin from the mitochondrial matrix, and plays a crucial role in hemoglobin synthesis and antioxidative stress. Participates in the early step of the heme biosynthetic process during insertion of iron into protoporphyrin IX (PPIX). Involved in the stabilization of the iron transporter mitoferrin-1/SLC25A37. In addition may be involved in mitochondrial unfolded protein response (UPRmt) signaling pathway, although ABCB10 probably does not participate in peptide export from mitochondria.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>biliverdin IXalpha(in) + ATP + H2O = biliverdin IXalpha(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:82359"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57991"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:82360"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCB family. Mitochondrial peptide exporter (TC 3.A.1.212) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CHG7"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015421">
        <property type="term" value="F:ABC-type oligopeptide transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090374">
        <property type="term" value="P:oligopeptide export from mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18573">
        <property type="entry name" value="ABC_6TM_ABCB10_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03249">
        <property type="entry name" value="ABC_MTABC3_MDL1_MDL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000048">
        <property type="entry name" value="ATP-binding cassette sub-family B member 10, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000403">
        <property type="entry name" value="ATP-binding cassette sub-family B member 8, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394:SF1">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY B MEMBER 10, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394">
        <property type="entry name" value="ATP-DEPENDENT PERMEASE MDL1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002773">
        <property type="entry name" value="ABC_prm/ATPase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0007">Acetylation</keyword>
    <keyword evidence="8 24" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="11" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="107"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="151"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="254"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="351"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="393"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="23">
        <location>
            <begin position="110"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="22">
        <location>
            <begin position="451"/>
            <end position="690"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00052250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00055589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00072683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00075187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00083334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="KAJ8045363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="696" mass="77014" checksum="2E28762E17297027" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CI45</accession>
    <name>A0A9Q1CI45_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Thyrostimulin alpha-2 subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_08314</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycoprotein hormones subunit alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CI45"/>
    <dbReference type="OrthoDB" id="6421717at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051427">
        <property type="term" value="F:hormone receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.90.10:FF:000049">
        <property type="entry name" value="Glycoprotein hormone alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006207">
        <property type="entry name" value="Cys_knot_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004133">
        <property type="entry name" value="DAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000476">
        <property type="entry name" value="Glyco_hormone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052680">
        <property type="entry name" value="Glyco_Hormone_Alpha"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31129">
        <property type="entry name" value="GLYCOPROTEIN HORMONE ALPHA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31129:SF2">
        <property type="entry name" value="GLYCOPROTEIN HORMONE ALPHA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03045">
        <property type="entry name" value="DAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00041">
        <property type="entry name" value="CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01185">
        <property type="entry name" value="CTCK_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01225">
        <property type="entry name" value="CTCK_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50277">
        <property type="entry name" value="GLYCO_HORMONE_ALPHA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040123408" evidence="8">
        <location>
            <begin position="25"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="CTCK" evidence="9">
        <location>
            <begin position="22"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS01225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8045325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="129" mass="14215" checksum="032EA05528627FC8" modified="2023-09-13" version="1">MNVEGQVVLLVSYVLLISIPCCKANDKNPVWRQPGCHLVGFAKLIEVDDCRTVTVIVNACRGYCLSYSYPSDYERYISGNGQQSVTSTGTCCSVHRTHDVDVVLSCIDNQQRMVTLRSAAQCSCSLCEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CJ12</accession>
    <name>A0A9Q1CJ12_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">WASH complex subunit 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_04821</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CJ12"/>
    <dbReference type="OrthoDB" id="10261210at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071203">
        <property type="term" value="C:WASH complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007032">
        <property type="term" value="P:endosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028191">
        <property type="entry name" value="WASH-4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028283">
        <property type="entry name" value="WASH-7_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028282">
        <property type="entry name" value="WASH-7_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027307">
        <property type="entry name" value="WASH7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31409">
        <property type="entry name" value="WASH COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31409:SF0">
        <property type="entry name" value="WASH COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14745">
        <property type="entry name" value="WASH-4_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14746">
        <property type="entry name" value="WASH-7_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14744">
        <property type="entry name" value="WASH-7_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WASH complex subunit 4 N-terminal" evidence="3">
        <location>
            <begin position="31"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="domain" description="WASH complex subunit 7 central" evidence="2">
        <location>
            <begin position="600"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="domain" description="WASH complex subunit 7 C-terminal" evidence="4">
        <location>
            <begin position="962"/>
            <end position="1124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1127"/>
            <end position="1177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1127"/>
            <end position="1141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8046211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1177" mass="136133" checksum="75BB98CE72BC7B4D" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CK19</accession>
    <name>A0A9Q1CK19_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NIDO domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_05783</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CK19"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003886">
        <property type="entry name" value="NIDO_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06119">
        <property type="entry name" value="NIDO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040277672" description="NIDO domain-containing protein" evidence="2">
        <location>
            <begin position="18"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="NIDO" evidence="3">
        <location>
            <begin position="59"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="195"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="250"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="256"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF06119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8046937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="309" mass="34658" checksum="7EA22ABEB4DAC958" modified="2023-09-13" version="1">MWSTTIIVACLSSYVASTPLMGHKENPENIIFFPGGEDAKLIELDRSIYNMREQNGGAVVNNNGYLSQDNLTTPKELRYLLYAYLSGVIDEDYVPPQIGVFKMSATTQRFTVLKTYFRELQDSSLEEAVRVLLRKGFDVSFDPDRILLGTWNQFCPRSVNGKVQSLVKTFECILAVNKNSSYAIFISDDTDPCLSTQSIPDEDNVPTEPKDLSSDLNSLDFMTSKGVMTPSSNETWNMTPEEDVSPLIYSDYEDNVPSNSSNPKQSHYESSQRPVSSQTQVSILETPLPWGGELLFPTKVRFLVPHSQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CKJ8</accession>
    <name>A0A9Q1CKJ8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_05876</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CKJ8"/>
    <dbReference type="OrthoDB" id="6502630at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8047006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="196" mass="21796" checksum="10FDB555E1DA2B87" modified="2023-09-13" version="1">MRYGKRKNPIVCGRGQRSSGVTGGQTLKTLLTRYLKMRYGKRKNPIIFDGGQRSSGVTGGRMLKTLLTRYLKMCYGKRKNSIVFDGGQRSSGVTGGRMLKTLLTRYLKMCYGKRKNPIIFGGGQRSSGVTGGRTLKTMLTRYLKMRYGKRKNPIIFGGGERSSGVTGGQTLKSSLTRYLKKVSSDGSQTWYIDAPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CL61</accession>
    <name>A0A9Q1CL61_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Mesoderm-specific transcript protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_06234</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CL61"/>
    <dbReference type="OrthoDB" id="7130006at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046464">
        <property type="term" value="P:acylglycerol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000041">
        <property type="entry name" value="Mesoderm-specific transcript homolog protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050266">
        <property type="entry name" value="AB_hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43798:SF33">
        <property type="entry name" value="HYDROLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G14860)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43798">
        <property type="entry name" value="MONOACYLGLYCEROL LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="domain" description="AB hydrolase-1" evidence="8">
        <location>
            <begin position="62"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8047268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="325" mass="36947" checksum="6714BCCE7BCBD9E1" modified="2023-09-13" version="1">MGPVASTIGAIVISLLAVFIMYPPPELSPRVTEWKDTGKYFTYREHSIFYHDSKGKGPTSDVLIIFHGYPTSSYDFHKIWNQLTERFGRVIAPDFLGYGLSDKPVQYDYSIVDQANMVEALLHKLGVNDVHILSHDMGDTVGQELIARYNEGTQKINIKSVCLMNGGIIPETIDFRWGQKMLLNPYLALVGMRLMNRRLFANGLGEVFGPHTNPTEEEFDDQWALIRYNDGNLVQHGIIQYLPQRFANRDRWVPALYQTKLPLHLIFGPSDPVNPAATFLVKYRELVKNSGVTELHADIGHYPQLEAPDDVVRGYFDFLDSLKKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CLB5</accession>
    <name>A0A9Q1CLB5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">NIPA-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_05875</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Required for proper positioning of a substantial amount of TPR at the nuclear basket (NB) through interaction with TPR.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CLB5"/>
    <dbReference type="OrthoDB" id="614844at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013909">
        <property type="entry name" value="NuBaID_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012935">
        <property type="entry name" value="NuBaID_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15835">
        <property type="entry name" value="NUCLEAR-INTERACTING PARTNER OF ALK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15835:SF6">
        <property type="entry name" value="ZINC FINGER C3HC-TYPE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08600">
        <property type="entry name" value="NuBaID_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07967">
        <property type="entry name" value="zf-C3HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3HC-type" evidence="8">
        <location>
            <begin position="62"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="NuBaID C-terminal" evidence="9">
        <location>
            <begin position="232"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="24"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8047005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="403" mass="45700" checksum="66118C5934C720B6" modified="2023-09-13" version="1">MAASQQRVQSKPRRIKTLLSSFLRKDPEQERVTSTSKPSDGDNSDTDKCDSQSSKPSVTVTPLNKENFLQRVDTYSTSTWFAKPLELSPLQCARYGWQNIDVDTLCCVSCKEVLYVSLPQPWQKEEYSSACDKVREQLQSAHGKICPWSGSPSPDSFVTISMANHKDQIFQYQQRVTQLVGFFTSLPCVEFSVLKDQPVESSVVLKCLTDFSKGRLDTTREQITDDEWNIVVMMALFGWQKKHSHSLPIVMCECCRKQAGLWNYASRETNLETVENTEDIESDFKTDKDDGEPPHKRIKKIKQSSFNPATEHRPWCPWICVNQASADSKLQKSPLRGDTEASNHPLWQSLLEILLCKADPSKFGSNSPLTNKVQTPPNNAWKAVRRLVSFWQSSEISTKTGTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CM59</accession>
    <name>A0A9Q1CM59_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">TBC1 domain family member 22B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_06866</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">May act as a GTPase-activating protein for Rab family protein(s).</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CM59"/>
    <dbReference type="OrthoDB" id="26371at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071889">
        <property type="term" value="F:14-3-3 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.750:FF:000009">
        <property type="entry name" value="TBC1 domain family member 22A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000001">
        <property type="entry name" value="TBC1 domain family member 22B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000004">
        <property type="entry name" value="TBC1 domain family, member 22B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.750">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957:SF26">
        <property type="entry name" value="LD44506P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="5">
        <location>
            <begin position="225"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="55"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="21"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="59"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="122"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="159"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00043879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8047778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="528" mass="60305" checksum="48C84499E44FA320" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CM65</accession>
    <name>A0A9Q1CM65_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Ubiquitin-conjugating enzyme E2 N</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_05976</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CM65"/>
    <dbReference type="OrthoDB" id="7851174at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd23813">
        <property type="entry name" value="UBCc_UBE2N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000015">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="5">
        <location>
            <begin position="4"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="3">
        <location>
            <position position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8047079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="150" mass="16997" checksum="F1969401FD5A0FCC" modified="2023-09-13" version="1">MSSGLPRRIIKETQRLMTEPVSGISAVPDEQNARYFHVHIAGPKDSPFEGGNFKLELFLPEDYPMAAPKVRFVTKIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLANDVAEQWKMNESKAIETAREWTRVHAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CMH3</accession>
    <name>A0A9Q1CMH3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_06198</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CMH3"/>
    <dbReference type="OrthoDB" id="38045at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021838">
        <property type="entry name" value="DUF3431"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43734:SF4">
        <property type="entry name" value="AMINE OXIDASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43734">
        <property type="entry name" value="PHYTOENE DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11913">
        <property type="entry name" value="DUF3431"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51971">
        <property type="entry name" value="Nucleotide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8047244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="643" mass="74719" checksum="609B125763786BD5" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CMX5</accession>
    <name>A0A9Q1CMX5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_06975</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CMX5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="116"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="191"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="204"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8047856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="330" mass="37179" checksum="83E2CA5B39DC6422" modified="2023-09-13" version="1">MDADINDEYCRFTRRTVSDPCISDTPEIAPFSSSYTDDAIDESHSDFRVLYSSQRKPGKDRTTSESPKPRVLKLVLKEPKKLPPPEDEGTDIDEEYYCYHKYYKGIIPLRDYYTYRPSRPADNSPAQQTTNDGPSSSQTGTHGPQLSASLSARLTDMALLDHVIELSKTDEGTDIDKAYYLYHHYFKGTMPPSDSHTHRPPSPEDNSSSPKANTDVPTARQTGLNRPGPSASSRARPTDMALLDRVIELSQTDEGTDLDQAYYRYHKCYKGVIPLSDYYTCRLSRADRNSPLRQTTTYVPAAQTGLHDMQPQEQALLSAQVLLNDRDYSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CPJ2</accession>
    <name>A0A9Q1CPJ2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_00760</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CPJ2"/>
    <dbReference type="OrthoDB" id="10066972at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33480">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33480:SF1">
        <property type="entry name" value="TYR RECOMBINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8048440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="177" mass="20240" checksum="E9A010422058D9CD" modified="2023-09-13" version="1">MQGAFDGMRKEGILMANQEQMKEATLVYERERRTGKEDPLVLCGLCKGFYPKICFSRYKVKCKGDGCSHACSVPLSLLSQESDITQGMSSYEQEILCSFGRDNAGQLCLIDPVIRTVGKRMWDKEKNTEVRKSVMTSMRRLASLYLAFKEQHKIHGSEELTRGNARDMFDRKHFVSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CQC5</accession>
    <name>A0A9Q1CQC5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein NLRC5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_00938</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CQC5"/>
    <dbReference type="OrthoDB" id="427518at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312">
        <property type="entry name" value="NACHT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312:SF2">
        <property type="entry name" value="NUCLEOTIDE-BINDING OLIGOMERIZATION DOMAIN-CONTAINING PROTEIN 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040132156" evidence="1">
        <location>
            <begin position="18"/>
            <end position="1168"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="129"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="NACHT" evidence="3">
        <location>
            <begin position="600"/>
            <end position="723"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8048574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1168" mass="135380" checksum="3BA139D1F842C8C5" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CQV0</accession>
    <name>A0A9Q1CQV0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CCR4-NOT transcription complex subunit 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_01455</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CNOT11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CQV0"/>
    <dbReference type="OrthoDB" id="10265389at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030014">
        <property type="term" value="C:CCR4-NOT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019312">
        <property type="entry name" value="CNOT11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15975">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15975:SF0">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10155">
        <property type="entry name" value="CNOT11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0943">RNA-mediated gene silencing</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="434"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="437"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8048939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="460" mass="51649" checksum="14A024DF6F946F62" modified="2023-09-13" version="1">MALSTKDMMYLTNLLTESAVNSSSFEVLAEGFHKHFPNKSDHFKVGNALVLLLRNQDLLPHADQRLAALFFLWEISKGEPISTSPFAPVFAHVLFSENERNQIVNLIPPIGKGEKVFISQLVSSSIPPRDVLALLKKTPHQIVSLDPGSNMVDWSSLKGSLAEKHAELPAESKAALSCIIADPETENSSMNSQSNVASVIDMLMCGNRSPINSHFKPEFIRVPPPLHICEDELVWLHPGERDHFIAWDNVMGINNSAGLQEVRKLMAKAFKEPLLLAKQQQLLSELEKDQKLVYHIGLTPAKFPGLVENNPIPAIEILLRLMQSNQITEYFSVLVNMDMSLHSMEVVNRLTTAVDLPQEFVHLYISNCISTCEAMEDRSMQNRHVRLVCVFLQSLIRNKIINVKDLFIEMQAFCIEFSHIREAAGLFRLLKNMDSDQQQQQPQPTQNNAPNATPSSTSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CQZ7</accession>
    <name>A0A9Q1CQZ7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">BRD4-interacting chromatin-remodeling complex-associated protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_02652</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2556847at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016514">
        <property type="term" value="C:SWI/SNF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052438">
        <property type="entry name" value="Chromatin_remod/trans_coact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015671">
        <property type="entry name" value="GSCR1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15572">
        <property type="entry name" value="GLIOMA TUMOR SUPPRESSOR CANDIDATE REGION GENE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15572:SF0">
        <property type="entry name" value="GLUTAMINE-RICH PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15249">
        <property type="entry name" value="GLTSCR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GLTSCR protein conserved" evidence="3">
        <location>
            <begin position="1490"/>
            <end position="1590"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="492"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="614"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="933"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="996"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1212"/>
            <end position="1248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1260"/>
            <end position="1311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1337"/>
            <end position="1362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1427"/>
            <end position="1454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1634"/>
            <end position="1670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1685"/>
            <end position="1736"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1761"/>
            <end position="1813"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1930"/>
            <end position="1994"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1086"/>
            <end position="1148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="492"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="504"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="534"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="550"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="573"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="619"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="933"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="996"/>
            <end position="1010"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1212"/>
            <end position="1229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1230"/>
            <end position="1246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1294"/>
            <end position="1309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1341"/>
            <end position="1353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1685"/>
            <end position="1709"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1780"/>
            <end position="1791"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1804"/>
            <end position="1813"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF15249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8049758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1994" mass="214022" checksum="EE80B5DC595F4B89" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CS94</accession>
    <name>A0A9Q1CS94_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_02335</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CS94"/>
    <dbReference type="OrthoDB" id="445826at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8049549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="191" mass="22207" checksum="A6F7A94B0C920F32" modified="2023-09-13" version="1">MLKTATRNAEKRWQKHKTDTNFIKFRNLRNNYKRHLEMNKTSYVHDKIMSCSNNSRALYQTVARLTGSMKSNPLPESTSDARLADDFAHYFYNKIDKIRLDLEQYALFDPPHRNVTKVKSFTALDEERVSKMIMKSKPTTGHLDPIPSSLIKLHCDIFTPIILSIINASFSSCKFHTSWKKCCSQTIVEKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CT41</accession>
    <name>A0A9Q1CT41_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_03162</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CT41"/>
    <dbReference type="OrthoDB" id="10601773at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8050094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="66" mass="7643" checksum="16E50092CBFBB866" modified="2023-09-13" version="1">MLTVTRNKMQLIHPICKDIVSHKEDFKQHKIVLTGSDSFLVEIDHGVIIKRQDMRATREEADTILV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CT63</accession>
    <name>A0A9Q1CT63_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NACHT, LRR and PYD domains-containing protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_04597</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8051142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CT63"/>
    <dbReference type="OrthoDB" id="120976at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312">
        <property type="entry name" value="NACHT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312:SF2">
        <property type="entry name" value="NUCLEOTIDE-BINDING OLIGOMERIZATION DOMAIN-CONTAINING PROTEIN 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040514137" evidence="1">
        <location>
            <begin position="20"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="domain" description="NACHT" evidence="2">
        <location>
            <begin position="215"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8051142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="705" mass="79335" checksum="EA09D437E3EBF1AC" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1GVR7</accession>
    <name>A0A9Q1GVR7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Arginyl-tRNA--protein transferase 1</fullName>
            <shortName evidence="5">Arginyltransferase 1</shortName>
            <shortName evidence="5">R-transferase 1</shortName>
            <ecNumber evidence="5">2.3.2.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Arginine-tRNA--protein transferase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_32762</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in the post-translational conjugation of arginine to the N-terminal aspartate or glutamate of a protein. This arginylation is required for degradation of the protein via the ubiquitin pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>an N-terminal L-alpha-aminoacyl-[protein] + L-arginyl-tRNA(Arg) = an N-terminal L-arginyl-L-aminoacyl-[protein] + tRNA(Arg) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10208"/>
            <dbReference type="Rhea" id="RHEA-COMP:9658"/>
            <dbReference type="Rhea" id="RHEA-COMP:9673"/>
            <dbReference type="Rhea" id="RHEA-COMP:10636"/>
            <dbReference type="Rhea" id="RHEA-COMP:10638"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78513"/>
            <dbReference type="ChEBI" id="CHEBI:78597"/>
            <dbReference type="ChEBI" id="CHEBI:83562"/>
            <dbReference type="EC" id="2.3.2.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the R-transferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.8" evidence="5"/>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1GVR7"/>
    <dbReference type="OrthoDB" id="74183at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004057">
        <property type="term" value="F:arginyl-tRNA--protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017137">
        <property type="entry name" value="Arg-tRNA-P_Trfase_1_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030700">
        <property type="entry name" value="N-end_Aminoacyl_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007472">
        <property type="entry name" value="N-end_Aminoacyl_Trfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007471">
        <property type="entry name" value="N-end_Aminoacyl_Trfase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21367">
        <property type="entry name" value="ARGININE-TRNA-PROTEIN TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21367:SF1">
        <property type="entry name" value="ARGINYL-TRNA--PROTEIN TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04377">
        <property type="entry name" value="ATE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04376">
        <property type="entry name" value="ATE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037207">
        <property type="entry name" value="ATE1_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="N-end aminoacyl transferase N-terminal" evidence="7">
        <location>
            <begin position="18"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="N-end rule aminoacyl transferase C-terminal" evidence="8">
        <location>
            <begin position="299"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="101"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="150"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="233"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="101"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="115"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="170"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR037207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8027582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="525" mass="59551" checksum="D6FD586F1D155320" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1GW31</accession>
    <name>A0A9Q1GW31_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Reverse transcriptase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_29844</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8027792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09274">
        <property type="entry name" value="RNase_HI_RT_Ty3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000115">
        <property type="entry name" value="Polyprotein of retroviral origin, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.370:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 17.6-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000032">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 297-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon gypsy-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054465">
        <property type="entry name" value="Integrase_p58-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041373">
        <property type="entry name" value="RT_RNaseH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22938">
        <property type="entry name" value="Integrase_p58_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17917">
        <property type="entry name" value="RT_RNaseH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="10">
        <location>
            <begin position="858"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase" evidence="9">
        <location>
            <begin position="1272"/>
            <end position="1449"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="327"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="591"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="692"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="78"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="327"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="342"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="366"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="591"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="635"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="660"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="707"/>
            <end position="731"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8027792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1664" mass="189840" checksum="A19335F20799C68A" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1GXZ1</accession>
    <name>A0A9Q1GXZ1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SWIM-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_32518</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1GXZ1"/>
    <dbReference type="OrthoDB" id="10510990at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04434">
        <property type="entry name" value="SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="2">
        <location>
            <begin position="450"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8027388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="666" mass="76783" checksum="9D3790B96C07C884" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1GZB3</accession>
    <name>A0A9Q1GZB3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Arginine--tRNA ligase, cytoplasmic</fullName>
            <ecNumber evidence="3">6.1.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Arginyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HOLleu_30751</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Nanhai2018</strain>
            <tissue evidence="17">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>tRNA(Arg) + L-arginine + ATP = L-arginyl-tRNA(Arg) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:20301"/>
            <dbReference type="Rhea" id="RHEA-COMP:9658"/>
            <dbReference type="Rhea" id="RHEA-COMP:9673"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78513"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.19"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.19" evidence="3"/>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1GZB3"/>
    <dbReference type="OrthoDB" id="68056at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017101">
        <property type="term" value="C:aminoacyl-tRNA synthetase multienzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004814">
        <property type="term" value="F:arginine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006420">
        <property type="term" value="P:arginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00671">
        <property type="entry name" value="ArgRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.730.10:FF:000016">
        <property type="entry name" value="Arginine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.70:FF:000002">
        <property type="entry name" value="arginine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000084">
        <property type="entry name" value="arginine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.70">
        <property type="entry name" value="Arginyl tRNA synthetase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00123">
        <property type="entry name" value="Arg_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001278">
        <property type="entry name" value="Arg-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005148">
        <property type="entry name" value="Arg-tRNA-synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036695">
        <property type="entry name" value="Arg-tRNA-synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035684">
        <property type="entry name" value="ArgRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008909">
        <property type="entry name" value="DALR_anticod-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00456">
        <property type="entry name" value="argS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11956:SF5">
        <property type="entry name" value="ARGININE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11956">
        <property type="entry name" value="ARGINYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03485">
        <property type="entry name" value="Arg_tRNA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05746">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00750">
        <property type="entry name" value="tRNA-synt_1d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01038">
        <property type="entry name" value="TRNASYNTHARG"/>
    </dbReference>
    <dbReference type="SMART" id="SM01016">
        <property type="entry name" value="Arg_tRNA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00836">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55190">
        <property type="entry name" value="Arginyl-tRNA synthetase (ArgRS), N-terminal 'additional' domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="8 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 13 17" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 13" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arginyl tRNA synthetase N-terminal" evidence="16">
        <location>
            <begin position="82"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="DALR anticodon binding" evidence="15">
        <location>
            <begin position="538"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM01016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ8028499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="664" mass="75810" checksum="B3516EB9C8411F15" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H0U1</accession>
    <name>A0A9Q1H0U1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">WD repeat-containing protein 55</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_30109</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat WDR55 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8027991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H0U1"/>
    <dbReference type="OrthoDB" id="2288928at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050505">
        <property type="entry name" value="WDR55/POC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44019">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44019:SF20">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24796">
        <property type="entry name" value="WDR55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="129"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="304"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="14"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="25"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8027991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="370" mass="41473" checksum="669702BB056B99CC" modified="2023-09-13" version="1">MEANNAAFPKDSDDATAEEVSPEQNEEKDKNEEQIDKLPKAVQLDLPAMGLEFHPKELLLAASFIDGTVKLYECLEQEETEELWSSKEFKKSSRAVQFSEDGSTLISISKDKSWKVFDVETGKTSKTYLKAHDVGIYSVAMAGANFVVTGDDDGRLKLWDLRQEKCVFEARENEDYLSDLAVDDNNKIVLATSGDGTLSSFNIKRRRFDLQSENLNNDLTCIELTRRGQKVVCGCSDGSLNFFNWGEFGDISDRFPGHPESIDFSCKIRDDIICTGSGDGLIRAVNLFPHRFLGVIGDHDDLPVNSLTIDSESVILASCGHDQKIKFWDVEHFHQLGKLEKRKSKASDVNKRLTSKGTQENFFSDLLDDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H1A1</accession>
    <name>A0A9Q1H1A1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Calcyphosin-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_28178</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8028920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H1A1"/>
    <dbReference type="OrthoDB" id="10225422at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058912">
        <property type="entry name" value="HTH_animal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21301">
        <property type="entry name" value="REVERSE TRANSCRIPTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21301:SF10">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26215">
        <property type="entry name" value="HTH_animal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="25"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="452"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8028920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="548" mass="62483" checksum="7FE3887130C08212" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H1S5</accession>
    <name>A0A9Q1H1S5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Fibronectin type-III domain-containing protein 3A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_26707</name>
    </gene>
    <organism evidence="4 5">
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            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
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                <person name="Chen T."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
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        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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        <source>
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        </source>
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</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H295</accession>
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        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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            <text>a phenyl acetate + H2O = a phenol + acetate + H(+)</text>
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            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:33853"/>
            <dbReference type="ChEBI" id="CHEBI:140310"/>
            <dbReference type="EC" id="3.1.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6 8">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="6 8">Binds 2 calcium ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the paraoxonase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.2" evidence="8"/>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8031154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H2K8"/>
    <dbReference type="OrthoDB" id="423498at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
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        <property type="term" value="F:arylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002640">
        <property type="entry name" value="Arylesterase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051288">
        <property type="entry name" value="Serum_paraoxonase/arylesterase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11799">
        <property type="entry name" value="PARAOXONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11799:SF12">
        <property type="entry name" value="PARAOXONASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01731">
        <property type="entry name" value="Arylesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01785">
        <property type="entry name" value="PARAOXONASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63829">
        <property type="entry name" value="Calcium-dependent phosphotriesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0106">Calcium</keyword>
    <keyword evidence="3 7 8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="113"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="271"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="disulfide bond" description="In form B" evidence="7">
        <location>
            <begin position="40"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602640-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR602640-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR602640-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU368025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8031154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="374" mass="41734" checksum="7C6D3B043906B2CF" modified="2023-09-13" version="1">MLWKAIAILLALLVTRHFVNLALLLGYHKTSLMNHWPGPCRTVPPVENGAEDIALTKSGLAIITSGIDINYRADATDPRISEAEGKLFAFDFNHPEKNAVELKFTGDFEFVPHGIGIWESADDGTFLFVVNHRRDAETIEIFKLSGTAAKPILEHVESVQDPLFTSMNDVVATGPRSFYVSNDGYLRYTVMRAVERLFNRPWGSVVHYNGKTARKVVDSAFEYNGMSLSRDGKFLFISCPFALSIAIYERSTDSNDLSFQQDIHVGTFPDNIFVHPGTGDLWIGCSPIGHRVFAAFGNLDNWAPSQAIRVRPLGTDAKPFEKFKLYEVYYDDAHLMSSSSSAVVVGNKLLVGSIVHKLVFCDMKTETNLPRDQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H2X8</accession>
    <name>A0A9Q1H2X8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL15m</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">39S ribosomal protein L15, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_23449</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL15 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H2X8"/>
    <dbReference type="OrthoDB" id="361383at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01341">
        <property type="entry name" value="Ribosomal_uL15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030878">
        <property type="entry name" value="Ribosomal_uL15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021131">
        <property type="entry name" value="Ribosomal_uL15/eL18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036227">
        <property type="entry name" value="Ribosomal_uL15/eL18_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005749">
        <property type="entry name" value="Ribosomal_uL15_bac-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12934">
        <property type="entry name" value="50S RIBOSOMAL PROTEIN L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12934:SF11">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL15M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00828">
        <property type="entry name" value="Ribosomal_L27A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52080">
        <property type="entry name" value="Ribosomal proteins L15p and L18e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 8" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL15/eL18" evidence="7">
        <location>
            <begin position="93"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="24"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="31"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="43"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8033267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="294" mass="33821" checksum="24C524B1E6973CDE" modified="2023-09-13" version="1">MGSTSNRKALEMVRYLPRVSFENIRDNPGAIKKHRQRGRGGKRGGESGRGRSGQGKRDTRPPIGYEGGTTPFYLRIPMFPFYKNFATRRQYLPVSMRKLQHLIDMGRINPEEPIDVTSFVHSGAVKINVLDKVYGIHLTDEGVDTFETPINIEVQWSSELSIAAIERCGGVFTLGYYDPQSLDILAHPLQFFRRGRPIPKRLLPPKDLVDMYTDPAKRGYLADPEKIREERLLLAQKFGYELPDITRSSRRALLQMRKDPRQIFFGLQPGWFVNMTDKTVIKPLDEELNEYYKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H311</accession>
    <name>A0A9Q1H311_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Paraoxonase</fullName>
            <ecNumber evidence="8">3.1.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_27794</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a phenyl acetate + H2O = a phenol + acetate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17309"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:33853"/>
            <dbReference type="ChEBI" id="CHEBI:140310"/>
            <dbReference type="EC" id="3.1.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6 8">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="6 8">Binds 2 calcium ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the paraoxonase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.2" evidence="8"/>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8031153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H311"/>
    <dbReference type="OrthoDB" id="423498at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004064">
        <property type="term" value="F:arylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002640">
        <property type="entry name" value="Arylesterase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051288">
        <property type="entry name" value="Serum_paraoxonase/arylesterase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11799">
        <property type="entry name" value="PARAOXONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11799:SF12">
        <property type="entry name" value="PARAOXONASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01731">
        <property type="entry name" value="Arylesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01785">
        <property type="entry name" value="PARAOXONASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63829">
        <property type="entry name" value="Calcium-dependent phosphotriesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0106">Calcium</keyword>
    <keyword evidence="3 7 8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="112"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="269"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="disulfide bond" description="In form B" evidence="7">
        <location>
            <begin position="39"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602640-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR602640-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR602640-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU368025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8031153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="373" mass="41817" checksum="B6C4BDF090B04B59" modified="2023-09-13" version="1">MLWKTIKILVALFVARHVVKLALLLGFHKTPLNHWPGPCRTVPPIEHGAEDIALTKSGLAIITSGIDVAYRGDLSDPRISEAEGKLFAFDFNHPEKNAVELKFSGNFEFVPHGIGLWESADDGTYVFVVNHRQEAETIEIFKLSGTTEKPVLEHVESVQDPLFTSMNDVVATGPRSFYVSNDGYLRFTAMRAVERLFNRPWGSVVHYNGKIARKVVDSAFEYNGMSLSRDGNFLFISCPFTSSVTVYERSTDSNDLSFHQEIQIGALPDNIFVHPETGDLWIGSSPIGHRVLAAFENLDNWAPSQAIRVRPLGSKEEPFEKFKLYEAFYDDGYLMSTSSSVAVVGNRMLVGSVMHKLVFCEMKTETNLPRDQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H3A2</accession>
    <name>A0A9Q1H3A2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DNA-binding protein RFX2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_27501</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H3A2"/>
    <dbReference type="OrthoDB" id="10056949at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039779">
        <property type="entry name" value="RFX-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057321">
        <property type="entry name" value="RFX1-4/6/8-like_BCD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12619">
        <property type="entry name" value="RFX TRANSCRIPTION FACTOR FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12619:SF5">
        <property type="entry name" value="TRANSCRIPTION FACTOR RFX4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25340">
        <property type="entry name" value="BCD_RFX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RFX1-4/6/8-like BCD" evidence="2">
        <location>
            <begin position="1"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="285"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="285"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="296"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="307"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="343"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8030945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="438" mass="49022" checksum="86093CACA9534BE5" modified="2023-09-13" version="1">MLRRKTTLAHLAQATRGVLSSDEAIYHMKEDWEKRTEQNPASIQVAWSSYDEWGMDSNFILKKLESYCDLLKPGITVEDHIHWLEMLIKGITNEVKNEDERANAVRSLLLQWGYLSHLIIRDQTLRSAASFGSFHLLNLLYQEYMLYVTDIHQHQLQEITLWKRVFGSDSNVSSGFLTFPVVCDLEECILRDNPLHQSPNGPQSQASTLSQNHLFGNCQDDTLFGYPHGMIPIFSQSVSEFTNQSNLLLTNVAACNAPTPVPTGLLYPIVPMSYHVTTPVSNCPATFPPEQQKNPVRPKKLAKKQLKNMSVSTQGSATPRKGDKSRFIAGPQQGKLQTQQPSDKSEQRPQEHVKLVPEDGVSTRLMKVIPVQSESVGKGGDAKLKEKGEPLVIEVSIPESQDLSAMQGQVLVSEGGKSYIVDDVKNVEMDQGLQKQKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H3F5</accession>
    <name>A0A9Q1H3F5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">46 kDa FK506-binding nuclear protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_24723</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H3F5"/>
    <dbReference type="OrthoDB" id="8022339at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.340">
        <property type="entry name" value="Nucleoplasmin core domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041232">
        <property type="entry name" value="NPL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31025">
        <property type="entry name" value="SI:CH211-196P9.1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31025:SF9">
        <property type="entry name" value="SI:DKEY-286J15.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17800">
        <property type="entry name" value="NPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleoplasmin-like" evidence="2">
        <location>
            <begin position="5"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="124"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="181"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="208"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8031513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="690" mass="78129" checksum="28BE9663BB7C3A26" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H5I1</accession>
    <name>A0A9Q1H5I1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNase H type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_23375</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H5I1"/>
    <dbReference type="OrthoDB" id="5987091at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="CDD" id="cd09275">
        <property type="entry name" value="RNase_HI_RT_DIRS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052055">
        <property type="entry name" value="Hepadnavirus_pol/RT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33050">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33050:SF7">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8033210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="186" mass="20373" checksum="F7B19A4CF520B748" modified="2023-09-13" version="1">MTVRPTEDKTARSIQEFVTFRDSGNKTVRDLAQVIGLLVSLLPGILYGKLHYRNLQFYKIDSRGNFNSSVTLSCYSVEDLNWWMSNLPAAYMPISQSEPNMCISTDASSIGWGAYCATTGPKVGGGWSPSDSSLHINVLELLAILMGLTSLCSHLENKHIKIMCDNSTAVSYINAMGGTHSVKCNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H5T3</accession>
    <name>A0A9Q1H5T3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_23637</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H5T3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8033406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="83" mass="9959" checksum="2C9625A47B61AAE9" modified="2023-09-13" version="1">MSDFCHHVVRSWHLCINYLSEVLCGTGQPLKKEHFSNQGNCCRHLMYLFSMILINHYLCHVISYHIVLGLSYHIRWRRDKNIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H6W9</accession>
    <name>A0A9Q1H6W9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_21577</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H6W9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8034650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="62" mass="7125" checksum="3E03B1B6E46FBDAB" modified="2023-09-13" version="1">MSRGRSLLFLVEVKGHLRLNLQKPCKWLVITISHNSSDSHFSYLVYKICHVEGETLSILVEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H7Y4</accession>
    <name>A0A9Q1H7Y4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">DNA annealing helicase and endonuclease ZRANB3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_19655</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8035850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H7Y4"/>
    <dbReference type="OrthoDB" id="2801544at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004520">
        <property type="term" value="F:DNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031297">
        <property type="term" value="P:replication fork processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00085">
        <property type="entry name" value="HNHc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.380">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002711">
        <property type="entry name" value="HNH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003615">
        <property type="entry name" value="HNH_nuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036443">
        <property type="entry name" value="Znf_RanBP2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45766:SF3">
        <property type="entry name" value="DNA ANNEALING HELICASE AND ENDONUCLEASE ZRANB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45766">
        <property type="entry name" value="DNA ANNEALING HELICASE AND ENDONUCLEASE ZRANB3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01844">
        <property type="entry name" value="HNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00641">
        <property type="entry name" value="Zn_ribbon_RanBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00547">
        <property type="entry name" value="ZnF_RBZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90209">
        <property type="entry name" value="Ran binding protein zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01358">
        <property type="entry name" value="ZF_RANBP2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50199">
        <property type="entry name" value="ZF_RANBP2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5 11" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RanBP2-type" evidence="10">
        <location>
            <begin position="305"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="RanBP2-type" evidence="10">
        <location>
            <begin position="667"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="242"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="544"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="726"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="10"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="245"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="553"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="755"/>
            <end position="769"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8035850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1186" mass="132263" checksum="87F11E7E6BC61426" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H9W8</accession>
    <name>A0A9Q1H9W8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Protein odr-4-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_15819</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ODR-4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H9W8"/>
    <dbReference type="OrthoDB" id="21458at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029454">
        <property type="entry name" value="ODR-4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33966">
        <property type="entry name" value="PROTEIN ODR-4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33966:SF1">
        <property type="entry name" value="PROTEIN ODR-4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14778">
        <property type="entry name" value="ODR4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="445"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="48"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="49"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8038399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="469" mass="51779" checksum="DFB98337A6C1E7D0" modified="2023-09-13" version="1">MVKSILAEEFLQDYISSLCDASRQCTVGLIAGHKSSQKDYAVALLPTPSEDDDEEEEEPSEGRPKKQKKCMNTVNEQWVAEHARQVMRMLPGGLSVMGIYVAASEEELASSQSKQRQILFAIFKMFQKKQVMDVKNEDSLDWSILQISTKTKKLTCKTFNVADSKSIAKPAEWKFQSFVNSWSCVSCQLQVNLRIPFKECFRDCSHYSKFVVNGVENLLNAVRESMVLINGQLRDEKNAIETLLGKKKSGKKSAPSSASQSLDINAELLMESAVLGPSQQLESTIAGDTNIVGWIQAKGYISPRSTFGDVEKVIKEDIQRSLQVRFDLLAEELRQSSGDEEVKELDIEPPSIIATPQRVFVKLPNTPILVSDYVFQDEEPKDVSAHFLELLNISVIEEEVDFSCEQMPDESLLQQIETASSHSSDVASLDELLKPGGGGGNAMKYAGAAIAGVGAVLALGMSYLAISQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HA88</accession>
    <name>A0A9Q1HA88_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Arylsulfatase G</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_18902</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sulfatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HA88"/>
    <dbReference type="OrthoDB" id="103349at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004065">
        <property type="term" value="F:arylsulfatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050738">
        <property type="entry name" value="Sulfatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000917">
        <property type="entry name" value="Sulfatase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42693">
        <property type="entry name" value="ARYLSULFATASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42693:SF42">
        <property type="entry name" value="ARYLSULFATASE G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00884">
        <property type="entry name" value="Sulfatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14707">
        <property type="entry name" value="Sulfatase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Sulfatase N-terminal" evidence="7">
        <location>
            <begin position="62"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8037951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="347" mass="38629" checksum="3A2603E6A797093A" modified="2023-09-13" version="1">MGCTDEEGYSETDSVICPKDWFEIPGYSDLDCYLCQSDAGDNEPALPFLHGRKIVEQPLNMTYLSLRYIQAAEMFITERETTTPFILYVGLQHMHVPFFVMPEFAGKSARGDAYGDALLEMDWVIETIVKVIEDNDEAENTLIWVARGGGSTSKYTVWQGGHLMPALAYWPGTIEGGRVSDELVSMMDIYPTMASLAGIDMPGNRIYDGIDISNILLDEPFSSKNRVLYHPLYIYENGLYNGKMGAVQIDEYKAVYETGGIPECGGLFGPSTRHDPPLIFNVKEDPMESTPIDPGSSEYQSVLMKVNQALVDLEQSLLQDNTTVVDYTSNSCVMLCCNPLNVVCRCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HCW7</accession>
    <name>A0A9Q1HCW7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Transmembrane protein 59</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_15085</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM59 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HCW7"/>
    <dbReference type="OrthoDB" id="6371519at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022065">
        <property type="entry name" value="Uncharacterised_TMEM59"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28652">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 59-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28652:SF2">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 59-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12280">
        <property type="entry name" value="BSMAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <keyword evidence="3 10 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040254773" evidence="11">
        <location>
            <begin position="38"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="255"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="317"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8040708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="346" mass="39077" checksum="35320EA0A7B088CC" modified="2023-09-13" version="1">MWVRPITSSQQSKMASPARNTTGMLSILFVLFATVSATGTTTSPFEEVFEDVASCKGDCDKTYPLHTYPEPNDLKACYRGCRLFSMLDFVEEVFNENFTDTMKECRDSCGEAYVDPSQLLACELGCSNQLPYAQALHRQWAEEESKVHLLIPVLSVQSLWRGLLDTYRGLSFSYWSVYMQDENGQVYVFESSPELDVFTLNPEANRVSARNWINAETNLQAFHPQTSTDINGQTGYDDSASWLECVSRKSGLPRWLLSMTLFLSVLALIWLCFVTLMTAPEQRISTPKLSIYGDLEYLKKGPVSPYPIYIQSMVGKKHPLPSRDDDDDDDEPLPLKVDISAQQSLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HDF7</accession>
    <name>A0A9Q1HDF7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Interferon-induced very large GTPase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_11881</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class dynamin-like GTPase superfamily. Very large inducible GTPase (VLIG) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1597724at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030383">
        <property type="entry name" value="G_VLIG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057365">
        <property type="entry name" value="URGCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052986">
        <property type="entry name" value="VLIG_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14819:SF25">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_52, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14819">
        <property type="entry name" value="GTP-BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25496">
        <property type="entry name" value="URGCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25683">
        <property type="entry name" value="URGCP_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51717">
        <property type="entry name" value="G_VLIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VLIG-type G" evidence="3">
        <location>
            <begin position="720"/>
            <end position="968"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="526"/>
            <end position="553"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8041146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1679" mass="194705" checksum="762EEEA550792393" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HDW4</accession>
    <name>A0A9Q1HDW4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Zinc finger homeobox protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HOLleu_12554</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Nanhai2018</strain>
            <tissue evidence="17">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6417226at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000080">
        <property type="entry name" value="Zinc finger homeobox protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000064">
        <property type="entry name" value="Zinc finger homeobox protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000081">
        <property type="entry name" value="Zinc finger homeobox protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051968">
        <property type="entry name" value="ZnFinger_Homeobox_TR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45891">
        <property type="entry name" value="ZINC FINGER HOMEOBOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HGI5"/>
    <dbReference type="OrthoDB" id="411173at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016706">
        <property type="term" value="F:2-oxoglutarate-dependent dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015095">
        <property type="entry name" value="AlkB_hom8_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33332">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09004">
        <property type="entry name" value="ALKBH8_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8049032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="180" mass="20677" checksum="B9CEE00FD5AD24DD" modified="2023-09-13" version="1">MILSVGAPQGVSSPHGFFPLYTSDCQSISRDCSVIKYADDTVITGYLSDDIDSYATTITRFVQWCDDHLLHLNVSKTKELIIDFRCEPVTHVPIYVHGEQVEQVDKYKYLGTMLSNKLDWSSNAIIVHKKANQRLYFLRKLKNLRVDKTIMTLFYPLSKVFCCITSYFTNATEIDVKMLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HI72</accession>
    <name>A0A9Q1HI72_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_04359</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HI72"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8050967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="155" mass="17313" checksum="1AF2948B6F9360C7" modified="2023-09-13" version="1">MNETVIKSANKFIDGRLDYRKRLNYFFKDDIELLCVSFGVYNRSASVNILQQLTGKHTSNTTLVTFLCLAVVVSLFVGLICIMRRRGKNATCRNAKAAASCTTPPLKLPELDSSSKTIPAGPFQTYQRKDNFMFDIFMKKVGIIALVGNISCKSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HIS8</accession>
    <name>A0A9Q1HIS8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SCAN box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_00598</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HIS8"/>
    <dbReference type="OrthoDB" id="5975462at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF1">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8048329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="178" mass="20440" checksum="C18DC15275EF2462" modified="2023-09-13" version="1">MGKELGLTGKDLFTFAQDRDKAARNERRRQREHQKELELEVKKAAEEAKAKPSLGNDVYSILKKSPVPSFDDKVDDIECYFKRFERYAEAMQYPKDKMVALLSTVLTGKALNVYSRLSEALLKPYVLNANGFCRKFRNSKLDSDETYSQFIGKLSGYLKRWIDLSGVEKLTVNSLNLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0Y8W5</accession>
    <name>A0A9Q0Y8W5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_44391</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000843">
        <property type="protein sequence ID" value="KAJ8017917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0Y8W5"/>
    <dbReference type="OrthoDB" id="7608935at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46486">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46486:SF1">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8017917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="180" mass="20161" checksum="F452A9AC56F18076" modified="2023-09-13" version="1">MRWVTYNEGELKGILNGKRQFRMVLKREIPSFLFIGGSKAHIRYFGQPRRCGRCWQVGHDKAECPNERDEDVGQAPEPSQLGPKYSTQELEEVAHEVEQDFLASKQCEAGTVTPEADSPPSLPTEPREAAIMTVEEDLHLSSESVSVMSDDDDDEPPPKDPPSDQYRAQGLKRSSPSKSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YAD6</accession>
    <name>A0A9Q0YAD6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_42462</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000076">
        <property type="protein sequence ID" value="KAJ8019147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YAD6"/>
    <dbReference type="OrthoDB" id="8718740at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8019147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="191" mass="21090" checksum="CF74047228FCAAAB" modified="2023-09-13" version="1">MQCTVRRARPNVTLSFIARSVDGDRIITNDTMTMPEGLGYTTVVTTSNVFLYSPLLVLLVCTASSSPGLLKHNYSTVLVENSNKDISHVKAESIYIKRDTTLKLSCSNQNIGYFVWKKSNPPNYEEHEILLHSVFIGGEFTQILADDVVLGDNSSLVIYSSKVKHEGKYMCISGDGRTNGVAVYNVTMIGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YB40</accession>
    <name>A0A9Q0YB40_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transmembrane protease serine 9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_41075</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S1 family. CLIP subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8019463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YB40"/>
    <dbReference type="OrthoDB" id="5918597at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000002">
        <property type="entry name" value="Transmembrane protease serine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000068">
        <property type="entry name" value="transmembrane protease serine 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252">
        <property type="entry name" value="ACROSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252:SF7">
        <property type="entry name" value="HYALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040191479" evidence="3">
        <location>
            <begin position="19"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="4">
        <location>
            <begin position="28"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="5">
        <location>
            <begin position="161"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8019463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="547" mass="59595" checksum="1A8049D436FB6996" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YC29</accession>
    <name>A0A9Q0YC29_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_43198</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000229">
        <property type="protein sequence ID" value="KAJ8018686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YC29"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8018686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="88" mass="9147" checksum="76874CBF8F849BD1" modified="2023-09-13" version="1">MSVEPEPQPHPGAGSTHQSDQRLLSSTDHFAVANYAADDDNDSDLLGNAANINSDAKPSVKPAYRQHSDAPGPADISQSPDDGPVQPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YF50</accession>
    <name>A0A9Q0YF50_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_45011</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010001618">
        <property type="protein sequence ID" value="KAJ8017521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YF50"/>
    <dbReference type="OrthoDB" id="9993736at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001614">
        <property type="entry name" value="Myelin_PLP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01275">
        <property type="entry name" value="Myelin_PLP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8017521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="96" mass="10975" checksum="E69D6F4A6D020297" modified="2023-09-13" version="1">MYMYALLFRVATHRAAGTHLRNYWSHPSLQRCHEDIQLNVWLTVTASIIVVISLIHFLMALAANHTYTRLFHKDMPGSPKYSNNFGSPGLAESPIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YFS0</accession>
    <name>A0A9Q0YFS0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Solute carrier family 35 member F6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_38884</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CRT-like transporter family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YFS0"/>
    <dbReference type="OrthoDB" id="29773at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013936">
        <property type="entry name" value="CRT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012404">
        <property type="entry name" value="UCP036436"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13146">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13146:SF0">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER F6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08627">
        <property type="entry name" value="CRT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036436">
        <property type="entry name" value="UCP036436"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="122"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="166"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="211"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="243"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="267"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8021625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="329" mass="36816" checksum="E069685154DE77ED" modified="2023-09-13" version="1">MFIGESSCLLAFKLIVLYRMCRKLPLDIGEQKFNPIVMLPPAMCDMCATSLMYMGLTLTYPSIFQMLRGAVVIFTGLLSVAFLNRRLHAYQWWGIFLVFVGLIVVGISDVVEPDPSQSQRPITNLITGDLLIVIAQIITATQMVIEEKFLSKYDVQPLQAVGWEGVFGFTVLSLLLIPFYFITPCAIRIDIPDCRLEDAPDAFHQLKNNPIIVIATVGTILSIAFFNFAGISVTREMSATTRMVLDSLRTVVIWVVSMMVGWEHFNWIQLIGFLILLVGTFIYNDAVFMPLYRRYLGGGDLETTPLLSPEADGTINYNNSVDIDNATKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YGD3</accession>
    <name>A0A9Q0YGD3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Mucin-5B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_40628</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="160294at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19941">
        <property type="entry name" value="TIL"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="3"/>
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        </source>
    </evidence>
    <sequence length="715" mass="73505" checksum="ABB58460D5AFF998" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YHI6</accession>
    <name>A0A9Q0YHI6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Neuronal acetylcholine receptor subunit alpha-6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_37683</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YHI6"/>
    <dbReference type="OrthoDB" id="5920062at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005230">
        <property type="term" value="F:extracellular ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18989">
        <property type="entry name" value="LGIC_ECD_cation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040190611" evidence="2">
        <location>
            <begin position="20"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="268"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="293"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="321"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="363"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="3">
        <location>
            <begin position="47"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8022708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="388" mass="42581" checksum="AD3B38FFA082B82A" modified="2023-09-13" version="1">MTFFLKLLYLTLWIGCVLCDPIPSSVSPSPESDGVVDLGSEVILKLRLLSFYSTRVAPAEPVVLTYDIIPVKVVEVDVRRGMVTVDLWLQHYWQDPRLVWDTTVYSSEILFFSANEVFVPEMALQNGVMTRTLPDPPVIVYPNGSILHFPAVRAEIYCQLPSIPFTDVICPITMGPWSHHTRYTVLANFAPAIDASVVHNTGICSIGDSWSELGETTFDCCPGIEFAFINLNLSISCSDDGFNAPAAKSLNSAPKKTQTLPLDSTHKSSLPIVVAPFLVLLIFVLPQKATNRVFLGTIVFAALLFIWTTEVSPEERRFLQLSTIVAISATFASLVPCVFRSVGKPDTQIPEHGPKPSKIKNRVAIMIDMMLFAAVAIVLGICTAGTFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YIS8</accession>
    <name>A0A9Q0YIS8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Abnormal spindle-like microcephaly-associated protein-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_35645</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YIS8"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040163624" evidence="1">
        <location>
            <begin position="19"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8023278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="160" mass="17956" checksum="EEAF989215566A9D" modified="2023-09-13" version="1">MPCMVVITYVVTTLVNLAKYTPTAPSVLECPDSVSRILDLKMIYRETNPVVFTKAAMLLIAVAQEKSLQKILLGTFKINDKLHSLLNLQERKLKLKTRQMIAKSKKQGMSMTRSQSMTPSKKKTGAELSALLGSWEIYKTRFRGPIEALKCLMGAFNIKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YL45</accession>
    <name>A0A9Q0YL45_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Kelch-like protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_37060</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8024339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YL45"/>
    <dbReference type="OrthoDB" id="45365at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632:SF3">
        <property type="entry name" value="KELCH-LIKE PROTEIN 32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632">
        <property type="entry name" value="LD33804P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8024339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="94" mass="10103" checksum="F759B6A6764324A3" modified="2023-09-13" version="1">MNSTVLKGLYDEFNSVERYDPRADAWFCVAPMSICRDSVAVAVLGDQIYAAGGFNGTSYVSTVECYDPVAGEWSLSTPLNGARAGGCLVQLDLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YMG2</accession>
    <name>A0A9Q0YMG2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SCAN box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_35330</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8025195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YMG2"/>
    <dbReference type="OrthoDB" id="10063366at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF13">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCAN box" evidence="2">
        <location>
            <begin position="129"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="223"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="223"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="238"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="262"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8025195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="392" mass="44690" checksum="37B394843380F8EA" modified="2023-09-13" version="1">MEREREKEEREIQMQREKEAREHEFRLKQLELGVIKASDPKIGLDTGGFDVSKHVKFVPKFQEDNVEKFFNHFEKLGEQLKWPRDKWSILIQSNFTGKAQEVYSALSIEDSMDYDKVKKAILQAYELVPEAYRQKFRKYRKADTQTYVEFAYQKERHFDRWCASKKVSTFDTLRQLMLVEEFKRCVNDDIKTHLEENKADNLKEVANLADQYALTHKFGKVGQTQNKGQFSRPNKKYGNNSGITSNSGSHSGSISPTNSGKKQPESSRQKNDQASSNKAAFKDITCGFCHKKGHTMANCFTLAKLKETETASNALANVENSSEVVVTLRPNSNDKIKEEYLPFVSEGFISLDGNSAHPPVKIKILRDTGATQSLLLDGVLPLSDSTSTGQMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YMH7</accession>
    <name>A0A9Q0YMH7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Unconventional myosin-Id</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_35215</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8025102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YMH7"/>
    <dbReference type="OrthoDB" id="6108017at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005902">
        <property type="term" value="C:microvillus"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030048">
        <property type="term" value="P:actin filament-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01378">
        <property type="entry name" value="MYSc_Myo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.820:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.530:FF:000004">
        <property type="entry name" value="Unconventional myosin ID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010926">
        <property type="entry name" value="Myosin_TH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036072">
        <property type="entry name" value="MYSc_Myo1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140">
        <property type="entry name" value="MYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140:SF713">
        <property type="entry name" value="UNCONVENTIONAL MYOSIN ID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06017">
        <property type="entry name" value="Myosin_TH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51757">
        <property type="entry name" value="TH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7" id="KW-0505">Motor protein</keyword>
    <keyword evidence="4 7" id="KW-0518">Myosin</keyword>
    <keyword evidence="2 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myosin motor" evidence="8">
        <location>
            <begin position="9"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="domain" description="TH1" evidence="9">
        <location>
            <begin position="812"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="7">
        <location>
            <begin position="573"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="102"/>
            <end position="109"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8025102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1004" mass="115849" checksum="B700972DA29BFCF4" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YMT8</accession>
    <name>A0A9Q0YMT8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">KICSTOR complex protein ITFG2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_37041</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8024326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YMT8"/>
    <dbReference type="OrthoDB" id="9996127at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032006">
        <property type="term" value="P:regulation of TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031793">
        <property type="entry name" value="KICSTOR_ITFG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16317">
        <property type="entry name" value="INTEGRIN ALPHA REPEAT DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16317:SF1">
        <property type="entry name" value="KICSTOR COMPLEX PROTEIN ITFG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15907">
        <property type="entry name" value="Itfg2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="122"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="481"/>
            <end position="508"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8024326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="508" mass="57354" checksum="F331EC8C75FC62C2" modified="2023-09-13" version="1">MCCSAVPTVGNVKSVWPTMRTVVFVQRQELEFTGNLSPNALVLGDVDNDSAYELVVGSLDGDLLVFKGEETEPVASASGLGMITCVGIGDICHQGKNNIFTMSAEGYFYIFDIKVCDSEEETSKLDGNEESKEENQGEVKPEKKESHRLEEIHKQHLPANSKVVLLADIDLDNSAELVVGYTDRKVRAFRWQTFVCEKDGESSTYAKLVLLQTWVLPAQIGSLSLSRHSDNFPKLVVSQPGCNYAELSCDWVSRSKFKEPSEEIKPVIHHPITNKRARNANISTEIIANVSKGDEKTQISDHLGSDRFAMCTLDGTLMLIEEDQILWSLLVDHQLFAITKLDIMCDGHDEVVSCALDGETYIVDHNRQSVRFHFEESVCAFAAGLYSMDGITNPPCLVYGTFNNKIFIYWNVRLPRISSLTLLDVIRDREEFKEKLKEEGIQDNNQEGMRKIVQYYLYEKPALMKYQQKQKVADKDLTVCKEDQDKEISKLTEQFEQTKVAAENKEEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YNN9</accession>
    <name>A0A9Q0YNN9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sulfotransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_33406</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YNN9"/>
    <dbReference type="OrthoDB" id="10010208at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053259">
        <property type="entry name" value="Golvesin-related_Golgi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005331">
        <property type="entry name" value="Sulfotransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32301">
        <property type="entry name" value="COUNTIN RECEPTOR CNR3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32301:SF6">
        <property type="entry name" value="GOLVESIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03567">
        <property type="entry name" value="Sulfotransfer_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8025762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="357" mass="41254" checksum="F75559F0CFB08C15" modified="2023-09-13" version="1">MERAKSLSHIEWATTACNEGRAVVREKKMGSLEVKIFDERVLCSASKHVTSSDNPFRGGSVQDFPCLDVAGVKTCDSLPNFFLYSNGQNQAYKDSVISYMHVPKCGGTTVKQCMGIVAKRENKENPYLLHLERRFEIELTPMQTHCPYSMFMGSDSLGICRKVKDKECSYFTFVREPYDRLVSLYFECRLQNDPPGKGRDNCMNLPITEWVMKVKHALFFFQLHRRVECPTGKMDGCEFVTPESAFELANNITYVNYIAEHLDKIFAVIGLQEDYHQSLKLLERAYGMQFYNVCKGLWMNRGKYGEGDIESNERKRQMLAVTLQGNEEVRKFIFPDVVLYEKAKEIFQKEIQRLKTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YR76</accession>
    <name>A0A9Q0YR76_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">F-box only protein 47</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_36332</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YR76"/>
    <dbReference type="OrthoDB" id="9858120at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056622">
        <property type="entry name" value="ARM_FBXO47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038946">
        <property type="entry name" value="FBXO47"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34098">
        <property type="entry name" value="F-BOX ONLY PROTEIN 47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34098:SF1">
        <property type="entry name" value="F-BOX ONLY PROTEIN 47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24467">
        <property type="entry name" value="ARM_FBXO47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="34"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8023791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="437" mass="50629" checksum="2E7DDF64179F5816" modified="2023-09-13" version="1">MKVTRNPLKTKHPQQDSCGCSHPHKRCRFQAPVVGYLHMLPNELIFKIFHLTSAEDLSRVSITSVTLSQLVRSYISCNNGLRHFLTKYILLLKDRNKDDKDIRLWDLFKRLGLLLKRSTFLLPTRERLMMMGRFVTKVYAILQLEVLQNLQCFGHLYGKFTRGWSEKECRLAFLTVMEYFKIKPKLVAVLSSSPGTFPKAEYETRCLLRHVFLTGYTQVYDLQTYLDLILKPYPLVHQAKILYLLYGPADSKRGVIGWYQMSEALLDARRSMHHFSGMAIALKSLYSHPAWTEGSVNSVLKELSDCPQEWTTLNMAKLLILCGEDICFNFLVSIALNGKMREVANIFVTLSMIIKEEDLQMKWLLNLVYKVCRALKNTHDVHSLLNAIPELYKDLTVELQEVPDAENYLNFADLAESQAELLRELLHHSYKMNRLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YRB8</accession>
    <name>A0A9Q0YRB8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_32241</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YRB8"/>
    <dbReference type="OrthoDB" id="10069752at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8027175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="133" mass="15115" checksum="09E8F450420F7BFD" modified="2023-09-13" version="1">MGFSSSYDDVSRNEKNAANGAAPEMLGEETDASKTIVLFAGDNVDHNILTIDGKGTFHGMGMIAAISPRRRTNWTIPRQNISDLNIVKRTKIEIKEHQFASYVHRNIQFRKLPTLCECDKRIDLLWELSFNFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YRX4</accession>
    <name>A0A9Q0YRX4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein Fer3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_35474</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8025302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YRX4"/>
    <dbReference type="OrthoDB" id="10048995at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032502">
        <property type="term" value="P:developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11415">
        <property type="entry name" value="bHLH_TS_FERD3L_NATO3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050283">
        <property type="entry name" value="E-box_TF_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23349">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, TWIST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23349:SF63">
        <property type="entry name" value="FER3-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BHLH" evidence="1">
        <location>
            <begin position="139"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8025302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="199" mass="22620" checksum="F3C32C748699BF59" modified="2023-09-13" version="1">MGNEFVSDFANTWSNGNQAVFDGYDPRENQSTEKGYLPQEPACLISCSPRRDAEFHASLSQGNSLVNLENFQGSLRQVVESENLSCQSQAVGLFSYQCQCVATKANSPNFKNTARENNLPVPTKHCLKSKKRRRKTTPAQRKAANIRERRRMFGLNDAFDQLRKKVPTFDHENKLSRIETLRLAIRYIGFMTDELQGPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YRY0</accession>
    <name>A0A9Q0YRY0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_33337</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YRY0"/>
    <dbReference type="OrthoDB" id="5800476at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000538">
        <property type="entry name" value="Putative Casein kinase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="17"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8025709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="135" mass="15155" checksum="23432C6A2A0FE5EF" modified="2023-09-13" version="1">MSGSASGKSEFIVGGKYRLVRKIGSGSFGDIYLGINQQNGEEVAVKLESFKAKHPQLLYESKLYRILQGGTGIPHIKWFGNENDYNVLVMDLLGPSLEDLFNFCNRRFSAKTVLMLADQVGVVRIYYYHKCHCGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YTZ0</accession>
    <name>A0A9Q0YTZ0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_32351</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YTZ0"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8027252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="69" mass="7884" checksum="27660362BD79305F" modified="2023-09-13" version="1">MATPWLRSAIPKKVVTLDFTWYLLRNSTGDTLCSIRFYIVPTLVTGTMECKHCIKCPKDTTASNEQFLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BAM1</accession>
    <name>A0A9Q1BAM1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="30">Phospholipase ABHD3</fullName>
            <ecNumber evidence="3">3.1.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="31">Abhydrolase domain-containing protein 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="34" type="ORF">HOLleu_40597</name>
    </gene>
    <organism evidence="34 35">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="34" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="34">Nanhai2018</strain>
            <tissue evidence="34">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="29">Phospholipase that may play a role in phospholipids remodeling. May selectively cleave myristate (C14)-containing phosphatidylcholines through its predominant phospholipase 1 activity, cleaving preferentially acyl groups in sn1 position. In parallel, may have a minor phospholipase 2 activity acting on acyl groups in position sn2. In addition to (C14)-containing phosphatidylcholines, may also act on other medium-chain-containing and oxidatively truncated phospholipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>1,2-ditetradecanoyl-sn-glycero-3-phosphocholine + H2O = 1-tetradecanoyl-sn-glycero-3-phosphocholine + tetradecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54456"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30807"/>
            <dbReference type="ChEBI" id="CHEBI:45240"/>
            <dbReference type="ChEBI" id="CHEBI:64489"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:54457"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>1,2-ditetradecanoyl-sn-glycero-3-phosphocholine + H2O = 2-tetradecanoyl-sn-glycero-3-phosphocholine + tetradecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54404"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30807"/>
            <dbReference type="ChEBI" id="CHEBI:45240"/>
            <dbReference type="ChEBI" id="CHEBI:131738"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="28">
            <dbReference type="Rhea" id="RHEA:54405"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>1-O-hexadecyl-2-nonadioyl-sn-glycero-3-phosphocholine + H2O = nonanedioate + 1-O-hexadecyl-sn-glycero-3-phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54552"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:64496"/>
            <dbReference type="ChEBI" id="CHEBI:78208"/>
            <dbReference type="ChEBI" id="CHEBI:138269"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:54553"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>1-hexadecanoyl-2-(5-oxopentanoyl)-sn-glycero-3-phosphocholine + H2O = 5-oxopentanoate + 1-hexadecanoyl-sn-glycero-3-phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40483"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16120"/>
            <dbReference type="ChEBI" id="CHEBI:72998"/>
            <dbReference type="ChEBI" id="CHEBI:77890"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:40484"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>1-hexadecanoyl-2-(9-oxononanoyl)-sn-glycero-3-phosphocholine + H2O = 9-oxononanoate + 1-hexadecanoyl-sn-glycero-3-phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41179"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:61042"/>
            <dbReference type="ChEBI" id="CHEBI:72998"/>
            <dbReference type="ChEBI" id="CHEBI:77812"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:41180"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>1-hexadecanoyl-2-glutaroyl-sn-glycero-3-phosphocholine + H2O = glutarate + 1-hexadecanoyl-sn-glycero-3-phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41159"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30921"/>
            <dbReference type="ChEBI" id="CHEBI:72998"/>
            <dbReference type="ChEBI" id="CHEBI:77756"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:41160"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>1-hexadecanoyl-2-nonadioyl-sn-glycero-3-phosphocholine + H2O = nonanedioate + 1-hexadecanoyl-sn-glycero-3-phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41388"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:72998"/>
            <dbReference type="ChEBI" id="CHEBI:78207"/>
            <dbReference type="ChEBI" id="CHEBI:78208"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:41389"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>1-octadecanoyl-2-acetyl-sn-glycero-3-phosphocholine + H2O = 1-octadecanoyl-sn-glycero-3-phosphocholine + acetate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54408"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:73858"/>
            <dbReference type="ChEBI" id="CHEBI:75220"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:54409"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>1-octadecanoyl-2-hexanoyl-sn-glycero-3-phosphocholine + H2O = hexanoate + 1-octadecanoyl-sn-glycero-3-phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54464"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17120"/>
            <dbReference type="ChEBI" id="CHEBI:73858"/>
            <dbReference type="ChEBI" id="CHEBI:138212"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:54465"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>1-octadecanoyl-2-nonanoyl-sn-glycero-3-phosphocholine + H2O = nonanoate + 1-octadecanoyl-sn-glycero-3-phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54472"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:32361"/>
            <dbReference type="ChEBI" id="CHEBI:73858"/>
            <dbReference type="ChEBI" id="CHEBI:138214"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:54473"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>1-octadecanoyl-2-octanoyl-sn-glycero-3-phosphocholine + H2O = 1-octadecanoyl-sn-glycero-3-phosphocholine + octanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54468"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:25646"/>
            <dbReference type="ChEBI" id="CHEBI:73858"/>
            <dbReference type="ChEBI" id="CHEBI:138213"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:54469"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>1-octadecanoyl-2-pentanoyl-sn-glycero-3-phosphocholine + H2O = pentanoate + 1-octadecanoyl-sn-glycero-3-phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54460"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:31011"/>
            <dbReference type="ChEBI" id="CHEBI:73858"/>
            <dbReference type="ChEBI" id="CHEBI:138211"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:54461"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>1-tetradecanoyl-2-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycero-3-phosphocholine + H2O = 2-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycero-3-phosphocholine + tetradecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54400"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30807"/>
            <dbReference type="ChEBI" id="CHEBI:76085"/>
            <dbReference type="ChEBI" id="CHEBI:86162"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:54401"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>1-tetradecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphocholine + H2O = 2-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-sn-glycero-3-phosphocholine + tetradecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54396"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30807"/>
            <dbReference type="ChEBI" id="CHEBI:76079"/>
            <dbReference type="ChEBI" id="CHEBI:86102"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:54397"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>1-tetradecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine + H2O = 1-tetradecanoyl-sn-glycero-3-phosphocholine + (9Z,12Z)-octadecadienoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54392"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30245"/>
            <dbReference type="ChEBI" id="CHEBI:64489"/>
            <dbReference type="ChEBI" id="CHEBI:86094"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:54393"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>1-tetradecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine + H2O = 2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine + tetradecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54388"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30807"/>
            <dbReference type="ChEBI" id="CHEBI:76084"/>
            <dbReference type="ChEBI" id="CHEBI:86094"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:54389"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1-acyl-sn-glycero-3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15801"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.4"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:15802"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AB hydrolase superfamily. AB hydrolase 4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="34">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.4" evidence="3"/>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BAM1"/>
    <dbReference type="OrthoDB" id="247542at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008126">
        <property type="term" value="F:acetylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006650">
        <property type="term" value="P:glycerophospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051792">
        <property type="term" value="P:medium-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051793">
        <property type="term" value="P:medium-chain fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000079">
        <property type="entry name" value="Abhydrolase domain-containing 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050960">
        <property type="entry name" value="AB_hydrolase_4_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012020">
        <property type="entry name" value="ABHD4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10794">
        <property type="entry name" value="ABHYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10794:SF63">
        <property type="entry name" value="ALPHA_BETA HYDROLASE 1, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005211">
        <property type="entry name" value="Ab_hydro_YheT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="35" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0719">Serine esterase</keyword>
    <keyword evidence="7" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="33">
        <location>
            <begin position="121"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="32">
        <location>
            <position position="211"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="32">
        <location>
            <position position="337"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="32">
        <location>
            <position position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00050145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00050182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00050195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00050276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00050674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00051164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00051705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00052087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00052144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00052588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00052747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00052808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00052894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00059841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00071303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00082158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="PIRSR" id="PIRSR005211-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="33">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="EMBL" id="KAJ8020886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="35">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="401" mass="44913" checksum="3EFE394F6D30622B" modified="2023-09-13" version="1">MAATPSSDTFQEWIPTLALLSVGSLYAAYYLKRVASKPIIACGNPKLQEFLQKHVPSIFQVYWPPFWCFQGHAHTILAVGFRIFISKTYRREVTETPDGGEIFLDWMDADDTSTINKARRPTVIIMPGLTGSSKESYIMHYADDMHRLGFRSVVFNQRGYGGSKLRVRGFMCSISQTPRGFCAGNTEDIHFVVSHVKKLYPDAPVAVIGLSIGGIILLNYLAEYGDKAPLVGALPVSAAWDLMKSNRSLEENLNWLLFNSYLTNNLRNYLKSHAHMIEDKYDVEHVLQSRTIYQFDDRVTAKMFGYRNADHYYTEASPYGKIDKIKIPTLCLNAADDPFAPLKTLPLTSASKSSHVAIAVPQYGGHLGFMQGTVPVGKTLISKVLSEFMSGVFNHPDDFLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BB82</accession>
    <name>A0A9Q1BB82_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_39822</name>
        <name evidence="1" type="ORF">HOLleu_43778</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000451">
        <property type="protein sequence ID" value="KAJ8018306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BB82"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8018306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8020270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="77" mass="8795" checksum="E2A7F44634BE52B3" modified="2023-09-13" version="1">MWFTFSIVTNALMEIMSARPKLHSVSGLITINRQFAVNLNTTLFHFTLISQIMDLKTYTVVCYVEASNLIPNVNRTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BC43</accession>
    <name>A0A9Q1BC43_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">40S ribosomal protein S14</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_37111</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uS11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BC43"/>
    <dbReference type="SMR" id="A0A9Q1BC43"/>
    <dbReference type="OrthoDB" id="1677536at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.80:FF:000002">
        <property type="entry name" value="40S ribosomal protein S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.80">
        <property type="entry name" value="Ribosomal protein S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01310">
        <property type="entry name" value="Ribosomal_uS11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001971">
        <property type="entry name" value="Ribosomal_uS11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018102">
        <property type="entry name" value="Ribosomal_uS11_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036967">
        <property type="entry name" value="Ribosomal_uS11_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007176">
        <property type="entry name" value="PRK09607.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11759">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S14/30S RIBOSOMAL PROTEIN S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00411">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002131">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00054">
        <property type="entry name" value="RIBOSOMAL_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8022266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="151" mass="16054" checksum="C8D26C75A353C636" modified="2023-09-13" version="1">MAPRKGKAVKEQAQVSLGPQVPEGENVFGVAHIFASFNDTFVHVTDLSGKETICRVTGGMKVKADRDEASPYAAMLAAQDVAAKCKEIGITALHIKLRATGGNKTKTPGPGAQSALRALARSGMKIGRIEDVTPIPSDSTRRKCGRRGRRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BC59</accession>
    <name>A0A9Q1BC59_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_38407</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BC59"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040334024" description="Transmembrane protein" evidence="1">
        <location>
            <begin position="24"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8021260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="81" mass="9037" checksum="80B52EE1E0BDDC71" modified="2023-09-13" version="1">MAANISPKEFVLFLFLLAHVCFSTIYFNGSPNVAKNAHFSLPKMQRKFHGDIGKIEEGLDPGDGYFSRIHGQCLTTLKHPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BD57</accession>
    <name>A0A9Q1BD57_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_40405</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BD57"/>
    <dbReference type="OrthoDB" id="10065844at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8020734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="113" mass="12841" checksum="073FEB2A6BD9F2AE" modified="2023-09-13" version="1">MPEVELEIFKGDLIKYHQFMKSFDSNVDCVCDESNLKLTRLMQYTAGLAKDAIRGCSLIGGDKGYRQDRSILETRFGNSHLVTEHIIHELRQRRQVWSPVDIQGLADGASNTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BD97</accession>
    <name>A0A9Q1BD97_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_35919</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BD97"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8023466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="202" mass="23627" checksum="B5CAAA2BFADCE92A" modified="2023-09-13" version="1">MANPSYIMLTLVVVIVLQFIVFVVAEGMQDESPLAKRRRLSIQRLRNSGRLRRIFKKSSDLPETEGYVEDDWVDPAEFLEFLDELNALERLKENSFESDKRGGERRKNGWLTGMFGKRSSPSIRQARNIPQAYLSGDYFGKRFTDSDENWKRAYNIDMPEKKRLLEDSGRQTTEIRRAYFRCLGGVDWPHSKEMSIQFAFNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BDC7</accession>
    <name>A0A9Q1BDC7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_40076</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BDC7"/>
    <dbReference type="OrthoDB" id="10050556at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="154"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="55"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8020477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="185" mass="20869" checksum="6218135E7BE4F113" modified="2023-09-13" version="1">MDIDSALNELGMLSAITDSRKDYQTEQEKSKESSCFHVVTANPQFVVNIGAVQHKTSSSKKSSQKVGESGDHTDSAKTDSSHLERAANDHRSYKVSGRLHHRYFFRPYLKNGSPRRHLGIVKEIQNRSRDGHFHIRLPPPAEATLTQAMASMNIERESTSSRPRNSLRNPPLDIRNRTGLRAHAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BDL5</accession>
    <name>A0A9Q1BDL5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_36355</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BDL5"/>
    <dbReference type="OrthoDB" id="10423313at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8023811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="221" mass="25423" checksum="D4AB013A0ABB09CE" modified="2023-09-13" version="1">MYFHFGRVTAVDFEEYLDGSPDPCLEVSGLESAVTIPFLTARPGGQPMYSLALAMFYDKDRRDFPKTNLIYPYIPVDDIRLDEEEAYVKDGEEEFRVSLLNQVPCQPPPSLVRYRLQDFINMNVQFGVPEPDFSFCDRHPMENRFCKNARKIGCTVHRHVPNICQIWLSDDQRACQTSVEIENITQKFRAHFLQDDGPVTIVASETYFGNYSVGLKMPCVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BDU5</accession>
    <name>A0A9Q1BDU5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Carbohydrate sulfotransferase 15</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_39027</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BDU5"/>
    <dbReference type="OrthoDB" id="8068875at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050659">
        <property type="term" value="F:N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019319">
        <property type="term" value="P:hexose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052654">
        <property type="entry name" value="CS_Sulfotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15723">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15723:SF0">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040225899" evidence="2">
        <location>
            <begin position="20"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="domain" description="Sulfotransferase" evidence="3">
        <location>
            <begin position="235"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="112"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="130"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="148"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8021738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="516" mass="60943" checksum="3F16536D2A0A5302" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BGH0</accession>
    <name>A0A9Q1BGH0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Cilia- and flagella-associated protein 43</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_33273</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="535167at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003341">
        <property type="term" value="P:cilium movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14885:SF1">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14885">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 43-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25828">
        <property type="entry name" value="CC_Cfap43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="12" id="KW-0969">Cilium</keyword>
    <keyword evidence="6 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="12" id="KW-0282">Flagellum</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="208"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="699"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1075"/>
            <end position="1107"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="779"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="847"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="911"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="1260"/>
            <end position="1287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="707"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="717"/>
            <end position="726"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8025655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1440" mass="163931" checksum="DA44CA2DA6D0EDD0" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BGZ4</accession>
    <name>A0A9Q1BGZ4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Tetraspanin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_36985</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8024287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BGZ4"/>
    <dbReference type="OrthoDB" id="438211at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03155">
        <property type="entry name" value="CD151_like_LEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1450.10">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF544">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00259">
        <property type="entry name" value="TMFOUR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="88"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="127"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="160"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="288"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="11"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8024287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="324" mass="36483" checksum="2A31CABE4BF87D47" modified="2023-09-13" version="1">MASFPVGDEGNESKDVLLQEFEKSGKAHENNQHTVPPEVDDTTPGDPHQPYHHQPSHPQHPRYTGRTESEMGSKYDDEPCCGISLLKYVLFIFNFFLLLAGAGVLAIGVWTLTSKTDYTELLCNNVYYFSVIVLIAAGALIMILAATGCYGAVQEIKGCLLIYFTILLLLCILEIGLSIFLYVFRAQLQVELENCLNHTLTRQYGKEGEEAFTENFDSLHRSFKCCGSFDYQDWKTSYWNASGLAGNRTTPDSCCKSESDYCSPRDHPSNIYRHGCVESLRVYLGDQIVIVMVVFLGIGLVEMIGLIFTMCLYCHLRYESEDMM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BI32</accession>
    <name>A0A9Q1BI32_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_34047</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8026254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BI32"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040423019" evidence="2">
        <location>
            <begin position="26"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="214"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="222"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="251"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8026254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="268" mass="31408" checksum="002A5BB3E172971B" modified="2023-09-13" version="1">MMHSMLKVALVVFSVALTFFNQCSTGGTALLDFSLEDEINEEMEAVRQMQEIIPEIECSQFIKGLQHLKKQTKCCKNTKRSQKEDSTCIGKNKFDICLYGIDVAVRYVPFFVEESKKYPDDTVIQNLSSNLQNFNMTFRDRLGRCIRPIENDCKSYELSNATDTECDTKSLVLEGMKDWTVKVIDLYDELNSTVNESSQSKLESRCKKEIKREIKRKRRELKKKERDQRQEKKNKRKRERDEKRRLKGQRQVDQQNDPMMTSSPPDCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BIR1</accession>
    <name>A0A9Q1BIR1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Endonuclease-reverse transcriptase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_32474</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BIR1"/>
    <dbReference type="OrthoDB" id="1001388at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1820">
        <property type="entry name" value="L1 transposable element, RRM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004244">
        <property type="entry name" value="Transposase_22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11505">
        <property type="entry name" value="L1 TRANSPOSABLE ELEMENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8027348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="493" mass="57011" checksum="678DF2065C7587E7" modified="2023-09-13" version="1">MHDDLRSLEINFEKAIEFFTKRVEDLESREKLNSDRIKALEDEVQKMKQVDLEQADRINVQERFSRRSNTRIVGFPCTENESCEGIVKSVMEKVGVSNVRIERAHRDGRLNPTRPRHILAKLSFYQDKITALKHQRRALENEPFFITDDLTKRDLQEKRKWASQVTQLYNQGTKLRFSAGKWRDSSGGDFNLVMNLDLDKTGGLPRTNFRARSKLIEIMNRHDLIDIWRERNLQSKSFTWHSNIDDSIHCRLDFFIISNHMKNLVANTLITSLFGSDHSSVSVTLRIGTIRGKGMWKLNTSLLGDPVYIDLIKRTIADTLNSDKGENVCLLWEACKVNIRSVSISYSKSLTITRRRDEQNLLDQISKLEQQNANFPSVFCHNKLTEARAALEALYDYKLKGTVVRSRARWSEEGEKNTKYFLNLEKRNRSMNSINELILSSGISISAHSDIMGEVKRFYSELYTKQNGISSIDFCSNHSVPHNKVSPEQQLVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BIW9</accession>
    <name>A0A9Q1BIW9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphatidylethanolamine-binding protein-like F40A3.3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_32570</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the phosphatidylethanolamine-binding protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BIW9"/>
    <dbReference type="OrthoDB" id="2506647at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="CDD" id="cd00866">
        <property type="entry name" value="PEBP_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.280.10">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008914">
        <property type="entry name" value="PEBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036610">
        <property type="entry name" value="PEBP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035810">
        <property type="entry name" value="PEBP_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001858">
        <property type="entry name" value="Phosphatidylethanolamine-bd_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11362:SF147">
        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11362">
        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01161">
        <property type="entry name" value="PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49777">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01220">
        <property type="entry name" value="PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8027426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="177" mass="19331" checksum="2CA4D17479F77234" modified="2023-09-13" version="1">MEQHEVVPDVLSATPPGVAEVTWPSGVKAELGNVVTPTQMQDIPQVSWDTESGALYTVIMTDPDAPSRSKPKYREWHHWLVHDVPGLDLSKGKTHAAYIGAGPPKGSGLHRYVILIYKQPGPLTIEEPAKPRSTAGRASWKANDFAAKYNLGSPVAANFVQAEWDDYVPKLYESLGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BJX0</accession>
    <name>A0A9Q1BJX0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Vacuolar protein sorting-associated protein 37A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_30145</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies. May be involved in cell growth and differentiation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Late endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VPS37 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BJX0"/>
    <dbReference type="OrthoDB" id="10260857at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000813">
        <property type="term" value="C:ESCRT I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043162">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11685">
        <property type="entry name" value="UEV_TSG101-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.660">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037202">
        <property type="entry name" value="ESCRT_assembly_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029012">
        <property type="entry name" value="Helix_hairpin_bin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009851">
        <property type="entry name" value="Mod_r"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13678">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13678:SF2">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 37A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07200">
        <property type="entry name" value="Mod_r"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140111">
        <property type="entry name" value="Endosomal sorting complex assembly domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51314">
        <property type="entry name" value="VPS37_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="5 7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="VPS37 C-terminal" evidence="9">
        <location>
            <begin position="329"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="131"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="153"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8028023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="415" mass="46963" checksum="C148D3F28DB2BF44" modified="2023-09-13" version="1">MASWFSYLAGDQKSSSTLPPVTALQEQRAKQISSLQNLNPNVVELKAQTEYRIPITHQGHNISLHIFLPPQFPQDKPVVSVTPPVRHPWVSPTMEVVGCPGLVSFGVHTDLGRVVQDIVREFNRNPPMVFPPASNQQPQMNNYPPYPPDSATSQVMPQPSSSFQPMPTLSGPPQNTYAPPFNTAGPCLGVTPSSNSISHIDFPQSVEAPEEASHTKSTTETDHAIFVNSHSPPPFLQGPSNSYQLPDIEDSFSVLGDMSSEQLQLILDDERKFMELFESMPGLKKFYEQRTELYRICQELAKKNLSYKPELEKRKERVRELEQEQVDLNETFHQQCLKQQALNKQCDPQLLLDNIRVAAADAECSSDSLAEDFLEGKLPLDDFLKQYSEKRTKFHLRKVKEEKLMQMLSDRSLRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BJX1</accession>
    <name>A0A9Q1BJX1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_30119</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BJX1"/>
    <dbReference type="OrthoDB" id="10040781at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8028000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="296" mass="34482" checksum="CA71421B80094D9D" modified="2023-09-13" version="1">MQHLKPSDAALEARDKLNQTTNRTLNTHSFDRAWQRVYGAKEYRPSWVGGLSQPRPRPAPSYRQKPILTVNDEPVVENLDEITANLPREEKKKVKEFYDLQVQGIPTEHRCKRAHEPLLISGKVVPREHELGRWREMITTDEVDEVTGEPVYNKVVKATNVPNWMKSNSYWKASSKGVIMPSERIDLLSPTPSPRPRENQVIHTARYDLVGKYENKIEPESLKLQGDRELPSKRELKTWGDQLEIPQRLEVDECITEMECSNISDRGRVSEKRRKFDSVLKEADVPRFFSHNNYWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BK56</accession>
    <name>A0A9Q1BK56_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_30255</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BK56"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8028112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="78" mass="8484" checksum="E14D08A77F6DF894" modified="2023-09-13" version="1">MESRALEYLTLLMGISLGASTWALALPSLNQTVIEGNVFSGIHRYCLQDVYACFRGGAADLRVTFSVELVRISACIEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BKB5</accession>
    <name>A0A9Q1BKB5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Copper transport protein ATOX1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Metal transport protein ATX1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_30434</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Binds and deliver cytosolic copper to the copper ATPase proteins. May be important in cellular antioxidant defense.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homodimer. Interacts with ATP7B. Interacts with ATP7A. Interacts (via dimer form) with SLC31A1 (via C-terminal domain); this interaction improves ATOX1 stability and controls intracellular Cu(I) levels.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the ATX1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BKB5"/>
    <dbReference type="OrthoDB" id="689350at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016531">
        <property type="term" value="F:copper chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006825">
        <property type="term" value="P:copper ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000008">
        <property type="entry name" value="Copper transport protein ATOX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051881">
        <property type="entry name" value="Copper_transport_ATOX1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017969">
        <property type="entry name" value="Heavy-metal-associated_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46365">
        <property type="entry name" value="COPPER TRANSPORT PROTEIN ATOX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46365:SF1">
        <property type="entry name" value="COPPER TRANSPORT PROTEIN ATOX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01047">
        <property type="entry name" value="HMA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0186">Copper</keyword>
    <keyword evidence="3" id="KW-0187">Copper transport</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="HMA" evidence="12">
        <location>
            <begin position="3"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00040962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00043201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00046351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8028248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="74" mass="8102" checksum="5BAA1257A2815C3F" modified="2023-09-13" version="1">MASKTYEYNVEMTCEGCSGAVDRILKRASDKVSDFEIDLPGKKVFVTTTVSSDEVLEMLKKSGKATKFVGEKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BKN9</accession>
    <name>A0A9Q1BKN9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Membrane-associated progesterone receptor component 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_30670</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cytochrome b5 family. MAPR subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BKN9"/>
    <dbReference type="OrthoDB" id="547796at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050577">
        <property type="entry name" value="MAPR/NEUFC/NENF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10281:SF106">
        <property type="entry name" value="IP06960P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10281">
        <property type="entry name" value="MEMBRANE-ASSOCIATED PROGESTERONE RECEPTOR COMPONENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="3">
        <location>
            <begin position="68"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="42"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8028443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="106" mass="12227" checksum="57AEA2D405B1E02E" modified="2023-09-13" version="1">MGRFHKFTCLRTYSRLLKMLQCTDRLQSAAIIPEDGKLADEDFLTGGRRKPEPPKQPQLPRMKRRDFTLQDLKKYNGVDDERILIAVNGQVFDVTRGKNFYGPDGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BMY6</accession>
    <name>A0A9Q1BMY6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Microtubule-associated protein 1B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_29182</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="5371837at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005875">
        <property type="term" value="C:microtubule associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007409">
        <property type="term" value="P:axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016358">
        <property type="term" value="P:dendrite development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031114">
        <property type="term" value="P:regulation of microtubule depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026074">
        <property type="entry name" value="MAP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057480">
        <property type="entry name" value="MAP1A/B/S-like_MBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056617">
        <property type="entry name" value="MAP1B/S_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13843:SF12">
        <property type="entry name" value="ATPASE F1_V1_A1 COMPLEX ALPHA_BETA SUBUNIT NUCLEOTIDE-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13843">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23415">
        <property type="entry name" value="MAPB1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25281">
        <property type="entry name" value="MBL_MAP1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Microtubule-associated protein 1B/S N-terminal" evidence="2">
        <location>
            <begin position="28"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="Microtubule-associated protein 1A/B/S-like MBL-like" evidence="3">
        <location>
            <begin position="222"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="507"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="718"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1068"/>
            <end position="1392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1407"/>
            <end position="1717"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="541"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="773"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="829"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="887"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="929"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1074"/>
            <end position="1088"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1105"/>
            <end position="1115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1127"/>
            <end position="1136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1144"/>
            <end position="1156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1170"/>
            <end position="1181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1204"/>
            <end position="1219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1237"/>
            <end position="1287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1310"/>
            <end position="1321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1323"/>
            <end position="1338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1348"/>
            <end position="1367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1482"/>
            <end position="1491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1492"/>
            <end position="1502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1505"/>
            <end position="1555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1560"/>
            <end position="1569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1622"/>
            <end position="1633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1675"/>
            <end position="1685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1692"/>
            <end position="1717"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8029718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1843" mass="201267" checksum="900DDC85764CE301" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BN21</accession>
    <name>A0A9Q1BN21_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_28989</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BN21"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8029559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="57" mass="6297" checksum="B248F1455C78894D" modified="2023-09-13" version="1">MCVCDALACKHDISRRVTVTELILGVWVGHIEKMIPIVFGGGQRSFGVRRGQNVKTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BNA1</accession>
    <name>A0A9Q1BNA1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_29000</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BNA1"/>
    <dbReference type="OrthoDB" id="6434825at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331:SF2">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040387589" evidence="1">
        <location>
            <begin position="23"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8029569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="228" mass="25810" checksum="4FE79803C127F8B5" modified="2023-09-13" version="1">MGALLAARTLVFVLKALKVSLPYKCYTDSTVVLSWIRNNSRKWKAFVANRVSEIHELVDPACWDHCPSESNPADLLTRGASVEKWLAAKFWFEGPEWLQVSEPNLSPVNLISVNQSGEEANSCPPYTLRNQENISFENPFNIERWGTFSKAVRIVAYVLSGKTVSLNSKIGKLTPFLGEDGLIRVKGRLQMSNLPYRERHPIILPKCHLIFLLVHFMHILLKHAGVEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BTC2</accession>
    <name>A0A9Q1BTC2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">VWF/SSPO/Zonadhesin-like cysteine-rich domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_25774</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BTC2"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014853">
        <property type="entry name" value="VWF/SSPO/ZAN-like_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08742">
        <property type="entry name" value="C8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VWF/SSPO/Zonadhesin-like cysteine-rich" evidence="1">
        <location>
            <begin position="87"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8032285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="159" mass="17682" checksum="C82119B136676E31" modified="2023-09-13" version="1">MLLHMGECIPDESFCYCIAEEKYALKDPNQYGDSYVTGNEMCNPKKGDGCHDGSAMKKSAESLCNGIINPFETLGLNCNDFFNNEELGEFFSECVTDMCATMLKTDFLCASYKTYADACRLKGGDPGAWWEFVPECGKSWELNSGNIYDIYIVAASLIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BZ52</accession>
    <name>A0A9Q1BZ52_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Estrogen-related receptor gamma</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_22438</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the nuclear hormone receptor family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BZ52"/>
    <dbReference type="OrthoDB" id="5799427at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06961">
        <property type="entry name" value="NR_DBD_TR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000006">
        <property type="entry name" value="Nuclear receptor subfamily 5 group A member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035500">
        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050200">
        <property type="entry name" value="Nuclear_hormone_rcpt_NR3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001723">
        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001628">
        <property type="entry name" value="Znf_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48092">
        <property type="entry name" value="KNIRPS-RELATED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00105">
        <property type="entry name" value="zf-C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01282">
        <property type="entry name" value="COUPTNFACTOR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00398">
        <property type="entry name" value="STRDHORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00047">
        <property type="entry name" value="STROIDFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00430">
        <property type="entry name" value="HOLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00399">
        <property type="entry name" value="ZnF_C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48508">
        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51843">
        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00031">
        <property type="entry name" value="NUCLEAR_REC_DBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51030">
        <property type="entry name" value="NUCLEAR_REC_DBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8 10 14" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Nuclear receptor" evidence="12">
        <location>
            <begin position="41"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="NR LBD" evidence="13">
        <location>
            <begin position="145"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="116"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ8035270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="386" mass="43981" checksum="6A39AC03E2E6A184" modified="2023-09-13" version="1">MTSEQALSQRDTVDLSVREHTDSTYTSNINKSQNEGSIGNVRLCLVCSDTATGCHYSVMTCEGCKGFFKRTVQKQLNYTCREGSRGCIIGKLSRNICQYCRFQKCLNVGMKTEAVREDRMPGGRPKAKRAKVKRDEPGKDLPLSEAEVGTSQLTEIPTEFDWQSVLQPVLDARPDLVPITDGLDADKCTLNEMMKVGYMELNMIIQWAKNIPDFTSISLEDRICLIKASFMDLSVLRVSYRSMDLPVGMLKYSSTLIFSEDRAIELGWQPELIKGNIEYVKRLKDLNLDPQEFAVMSVLVLFFPDAAGLKDKEKLTQLQSHYLECLRLYQLHRYKENGLKRYGKFLLRLPSLRIVSAKAMENFFSLRLDGKIEITDLVEEILEMTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BZP5</accession>
    <name>A0A9Q1BZP5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_22630</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BZP5"/>
    <dbReference type="OrthoDB" id="8963689at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="78"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8035406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="153" mass="17706" checksum="473C07DEE6248612" modified="2023-09-13" version="1">MPFNLDKFVASPSVEELDSLKKSEIVKVAKHYGIEFQPLMRKDEIKRYVLEYLVDESILPSTVLETAITVPTDNTFELKRLEIEMNKEVRLKELEKEEREMQMQREEKAREHEFRLKQLELGVIKGSDPKIGLDTGGFDVSKHVKFVPKISGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C127</accession>
    <name>A0A9Q1C127_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_17940</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C127"/>
    <dbReference type="OrthoDB" id="10078694at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8037188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="90" mass="10005" checksum="7867F582B8097849" modified="2023-09-13" version="1">MADDFIVGIVECAKCKDPLSFRVTRDVNDHPNVNVWMAEVAVKSLRQNKIRKTGVQVMCDHCGNVLGKEVHESGKPALELRPGSLVFDKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C1W3</accession>
    <name>A0A9Q1C1W3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Heparan sulfate glucosamine 3-O-sulfotransferase 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_17817</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C1W3"/>
    <dbReference type="OrthoDB" id="10062788at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008467">
        <property type="term" value="F:[heparan sulfate]-glucosamine 3-sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037359">
        <property type="entry name" value="NST/OST"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10605:SF72">
        <property type="entry name" value="HEPARAN SULFATE 3-O SULFOTRANSFERASE-B, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10605">
        <property type="entry name" value="HEPARAN SULFATE SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="domain" description="Sulfotransferase" evidence="8">
        <location>
            <begin position="187"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="61"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="62"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="91"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="active site" description="For sulfotransferase activity" evidence="3">
        <location>
            <position position="196"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="279"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="287"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="403"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR637359-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR637359-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR637359-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8037082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="459" mass="53148" checksum="1250EAD3480E9CEA" modified="2023-09-13" version="1">MNHQKLLLIIIFFSIILIFILGSYLTSHSAVTIFNKISPSFQLKARTEIWPSIQNFSWSIPKADDNEAHDTKTKPEAANNRQATGLAGSRQDSKYKKSQKTEVSDAQKFVQKSHGAENPSKKKDAAAKEVIFRIFANMDEELRDQYLIQVKKFESEHSTQINQLPVSMQRNFLEKFLKAAGYYQKLPDVINIGVKKSGTNALGFFIAHHPLISHSLGNEVHFFDWNYQKGLKFYRSKMGFTSKFQLSFEKTPRYFVTDSAPANILKDLGNSTKFVLCFRDPIDRLVSDFRHETELRLRKEWIKSGKKKMNSSPEKEGKKLEKLLIDKKGNINVSSDFIRTSSYSIHLKNWLKYFPLDNFIFLDHDRLLQDTFSVMKEVESFLDLKEFFRKDMFYFDPTRGGPCMHTDGKPCPSKSTPGFMPKAKLSKAVQAKLRVYFRPLLQELLNITGKRFPWSHSYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C3R0</accession>
    <name>A0A9Q1C3R0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_18474</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C3R0"/>
    <dbReference type="OrthoDB" id="8930602at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025398">
        <property type="entry name" value="DUF4371"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008906">
        <property type="entry name" value="HATC_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46880:SF5">
        <property type="entry name" value="DUF4371 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46880">
        <property type="entry name" value="RAS-ASSOCIATING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05699">
        <property type="entry name" value="Dimer_Tnp_hAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14291">
        <property type="entry name" value="DUF4371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4371" evidence="2">
        <location>
            <begin position="67"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="HAT C-terminal dimerisation" evidence="1">
        <location>
            <begin position="392"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8037614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="473" mass="54187" checksum="07593CFEE4679456" modified="2023-09-13" version="1">MKSAAHQQAMVAEDPSQQSLTEVKMKLNEQEKKSYIAAMKVVYWLATEEIANQKYPRLLNLLRVQGVKDVIQLKRGKNSTKESPTSFYLFLKSINEVILGRMLKEVQNSPFIGIGLDESTDRSNEKHVVFIIRYVGVDQVKTTFLKVACVDDGKALTLYQTMCKVLQEYKIPIKKITGLGIDGASVMLSDRNGVCGLLHRDNPFAVALHCVCHRLHLVLSSVTETLEEMKTDQGNGHFLKAFLETMHGDTNYYGIPISVKPGRGANNVSEREQFERDRVDFIDGIVANIKSRFPTVDLLEAMQIFDPSCYPPNNDQLNSWGVTYLETLLAHFGERHDTTNQVFDPLVDEMARGEFLVFKRLVAANKGDQEENNDFFQPQQLFKKIFGRQYPQNRKIYPEMFKLMSIAMCVMPSNAEAERAFSLQNRIKNKLRTRITTDHLDQLMRVAYCQLDLDSFDFQSALDHYSKSPHRWY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C4J9</accession>
    <name>A0A9Q1C4J9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PKD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_18696</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C4J9"/>
    <dbReference type="OrthoDB" id="8510435at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00146">
        <property type="entry name" value="PKD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045219">
        <property type="entry name" value="PKAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022409">
        <property type="entry name" value="PKD/Chitinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000601">
        <property type="entry name" value="PKD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035986">
        <property type="entry name" value="PKD_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11861">
        <property type="entry name" value="MELANOCYTE PROTEIN PMEL 17-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11861:SF8">
        <property type="entry name" value="PKD DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00801">
        <property type="entry name" value="PKD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00089">
        <property type="entry name" value="PKD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49299">
        <property type="entry name" value="PKD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50093">
        <property type="entry name" value="PKD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040224924" description="PKD domain-containing protein" evidence="4">
        <location>
            <begin position="23"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="343"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="PKD" evidence="5">
        <location>
            <begin position="180"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8037791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="432" mass="47997" checksum="355CCE9E3BF0DF75" modified="2023-09-13" version="1">MSIRLDVVFLVFLVGVVQRSVGDSLNPTEDLPYNVTVVADGPTLLGATTTFVATLDTGTDFQDTTYKYTWSEVFGYLIPRVKSTYSEENCTFSRPYTRPEDVRDHIMSVKVYRKSILWVMVAQGSVTFQVTAHIPAHVILSQNETVCNGPVCDVATNQSAQLTVSIHDPSSYLQNTISLFDWDFGDENQNPKTTSMSLSHVYSEIGCYNVSVNITAVQPSGSVKTGNAIVTLLLKDAIHNLTINGLEHVTVGDKKTINITCSGSPPISFCWSIFQANMSSSLPVMRSCIQTYLQEECQTEIDYSFNTSKEYILRVRATNDVSFQALTAEIRPREPPVLTSDGIAGLVVGICFGLTLTCISIGIISHQIHKERMRHVEVADFNFEASSRYDSIREHARRLNDAKKKPTEKTPLLNKNPTLDVRWVKGSETVEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C759</accession>
    <name>A0A9Q1C759_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Mitogen-activated protein kinase kinase kinase 8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_14159</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8039986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11584:SF369">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 19-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11584">
        <property type="entry name" value="SERINE/THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="185"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="91"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8039986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="311" mass="35190" checksum="F93832D279118E03" modified="2023-09-13" version="1">MWFILETSSLPQLLIFVASCFVLVAFLPCFIFRRLLSGTTCHNQNMPEKTPDLMEATSVRSLEGDFIREPLHTNPFEDVQALKQSNADENGNIQKQQKTLSGTESVSQGCITSSNRESVEASSCTEDSPKNRTEALTREVRQNCVIYSNNVSDVKSFSEPGYNDGVLMNQNLTVWNGQYRFNKEFLQDKMIGEGDYGIVIACHDWKTKGKIATKMKENLRPFEVSLLAEVKSMPFIIGFLGATTEGNILYLHMELFQGACHLEYFAQEGWKHLKSQVIVVQICLQLLQALDYLSRRQIIHRDLTGKYAAGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C7E2</accession>
    <name>A0A9Q1C7E2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Fibulin-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HOLleu_17101</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Nanhai2018</strain>
            <tissue evidence="16">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C7E2"/>
    <dbReference type="OrthoDB" id="9946071at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000038">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051505">
        <property type="entry name" value="C-type_lectin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14789">
        <property type="entry name" value="CHONDROLECTIN VARIANT CHODLFDELTAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2 11" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="6" id="KW-0430">Lectin</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="741"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="15">
        <location>
            <begin position="310"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="14">
        <location>
            <begin position="684"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="169"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="219"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="536"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="243"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="280"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="536"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="693"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="712"/>
            <end position="721"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ8039394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="876" mass="96287" checksum="00565C7AEC4BB2D1" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C9R5</accession>
    <name>A0A9Q1C9R5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DDB1- and CUL4-associated factor 15</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_15176</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C9R5"/>
    <dbReference type="OrthoDB" id="6354267at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080008">
        <property type="term" value="C:Cul4-RING E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20913">
        <property type="entry name" value="DCAF15-CTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20917">
        <property type="entry name" value="DCAF15-NTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038914">
        <property type="entry name" value="DCAF15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047319">
        <property type="entry name" value="DCAF15_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032734">
        <property type="entry name" value="DCAF15_WD40"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28541">
        <property type="entry name" value="DDB1- AND CUL4-ASSOCIATED FACTOR 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28541:SF1">
        <property type="entry name" value="DDB1- AND CUL4-ASSOCIATED FACTOR 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14939">
        <property type="entry name" value="DCAF15_WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDB1- and CUL4-associated factor 15 WD40 repeat-containing" evidence="2">
        <location>
            <begin position="48"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="295"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="466"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="519"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="295"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="309"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="466"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8040778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="859" mass="96097" checksum="7286F38AD603AF21" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CA46</accession>
    <name>A0A9Q1CA46_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Low-density lipoprotein receptor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_15314</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CA46"/>
    <dbReference type="OrthoDB" id="72419at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000241">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050778">
        <property type="entry name" value="Cueball_EGF_LRP_Nidogen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000033">
        <property type="entry name" value="LDLR_classB_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46513:SF13">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46513">
        <property type="entry name" value="VITELLOGENIN RECEPTOR-LIKE PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00058">
        <property type="entry name" value="Ldl_recept_b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00135">
        <property type="entry name" value="LY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63825">
        <property type="entry name" value="YWTD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51120">
        <property type="entry name" value="LDLRB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="repeat" description="LDL-receptor class B" evidence="6">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="6">
        <location>
            <begin position="46"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8040881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="152" mass="17732" checksum="4545FDA0B0743066" modified="2023-09-13" version="1">MYWTDWGTSPKIERAGMNGAYRETIIDKSKVNIHWPNGLTIDYHSDMLYWVDARYHLLATCDFNGERYRTILQDETALMHPFHISVFQDEVYWTDWDAYSVRRVNKFTGEGVTDVVGSLVRGTPNGLHIWHPSKQPEGECVILQASFFWNIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CCJ2</accession>
    <name>A0A9Q1CCJ2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">RNA-directed DNA polymerase from mobile element jockey</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_13169</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8042174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CCJ2"/>
    <dbReference type="OrthoDB" id="10056483at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020847">
        <property type="entry name" value="AP_endonuclease_F1_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33395:SF22">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33395">
        <property type="entry name" value="TRANSCRIPTASE, PUTATIVE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00726">
        <property type="entry name" value="AP_NUCLEASE_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="536"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8042174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="700" mass="79165" checksum="15B0F2643BA7C065" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CD56</accession>
    <name>A0A9Q1CD56_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Reverse transcriptase RNase H-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_09603</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8042756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CD56"/>
    <dbReference type="OrthoDB" id="427924at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09274">
        <property type="entry name" value="RNase_HI_RT_Ty3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon gypsy-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041373">
        <property type="entry name" value="RT_RNaseH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17917">
        <property type="entry name" value="RT_RNaseH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Reverse transcriptase RNase H-like" evidence="7">
        <location>
            <begin position="7"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase zinc-binding" evidence="8">
        <location>
            <begin position="214"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF17917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8042756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="265" mass="30324" checksum="99666B3B0D5D4865" modified="2023-09-13" version="1">MRIGVLTYLESTHSTVFTDHQALVWMLHTKSLKGRLIRWALHLQEFDYDIHYRPGVQNTIPDTLSRSPQIAAIEYDDNVCFHPSCRGDPSSRINWVQCDNCDNRFHCLCLGLTKKEADELDVCEQCSATARGTCVVNDKEPSSSKIDNESNVLPDINQFIVAQKKDKNLLQIISKLESTDQGSSSIHLKYVMEGGLLKRTDNVNKKDKIGKVVVPQNLVHEVITHFHCTPTCPHLGTRKTVSKICEKFWWDKVRREVEVFIKRCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CEY9</accession>
    <name>A0A9Q1CEY9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_10600</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CEY9"/>
    <dbReference type="OrthoDB" id="6141723at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051055">
        <property type="entry name" value="PIF1_helicase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47642">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47642:SF5">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8043493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="100" mass="11439" checksum="0BBB6CE52B6E1273" modified="2023-09-13" version="1">MDIQFVLDAYSCIMYILSYMTKAEHEMGSLLKQAQQEARDGNQDAVAELRRLGSIYLNHREVSIMEAVYRVTGMPLKQSSRQVLFLPTDPDSWKISLPLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CG93</accession>
    <name>A0A9Q1CG93_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_11240</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CG93"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040492680" evidence="2">
        <location>
            <begin position="20"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="131"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="132"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8043924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="558" mass="64647" checksum="EA7F1BDD8CA51E1C" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CGC3</accession>
    <name>A0A9Q1CGC3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Ovomucoid</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_07677</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CGC3"/>
    <dbReference type="OrthoDB" id="126772at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030414">
        <property type="term" value="F:peptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036058">
        <property type="entry name" value="Kazal_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21312:SF28">
        <property type="entry name" value="OVOINHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21312">
        <property type="entry name" value="SERINE PROTEASE INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00050">
        <property type="entry name" value="Kazal_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00280">
        <property type="entry name" value="KAZAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100895">
        <property type="entry name" value="Kazal-type serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51465">
        <property type="entry name" value="KAZAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Kazal-like" evidence="5">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8044816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="109" mass="12241" checksum="DA5867C0C4673645" modified="2023-09-13" version="1">MCWLECPKISRPVCGSNGRTYSNECMLIQDRECNGFRVKKEHDGPCERVFPMSISEYSKQQVRKRRLCNGKGLTVCSKMGGLCVKTDKGNELICQISNADVSFSQNIAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CGG8</accession>
    <name>A0A9Q1CGG8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Mitochondrial sodium/calcium exchanger protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_11393</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8044044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CGG8"/>
    <dbReference type="OrthoDB" id="407410at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005432">
        <property type="term" value="F:calcium:sodium antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.30">
        <property type="entry name" value="NCX, central ion-binding region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051359">
        <property type="entry name" value="CaCA_antiporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044880">
        <property type="entry name" value="NCX_ion-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12266:SF0">
        <property type="entry name" value="MITOCHONDRIAL SODIUM_CALCIUM EXCHANGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12266">
        <property type="entry name" value="NA+/CA2+ K+ INDEPENDENT EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0050">Antiport</keyword>
    <keyword evidence="4" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="4" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="29"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="118"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="157"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="215"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="360"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="419"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="451"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="480"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="518"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="555"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="588"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="9">
        <location>
            <begin position="125"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="9">
        <location>
            <begin position="453"/>
            <end position="602"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8044044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="611" mass="67671" checksum="038E51CA2CD6D4F3" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CGQ6</accession>
    <name>A0A9Q1CGQ6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_07019</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CGQ6"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8044304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="60" mass="6886" checksum="47403A5EF04B46CA" modified="2023-09-13" version="1">MSMACACRHYINLKMKLVRSALASLEMNRVLGACQDLWWTVWATAVMEQYLLVLGQLLIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CHK5</accession>
    <name>A0A9Q1CHK5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Retinol-binding protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_07418</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CHK5"/>
    <dbReference type="OrthoDB" id="1872155at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00742">
        <property type="entry name" value="FABP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012674">
        <property type="entry name" value="Calycin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000463">
        <property type="entry name" value="Fatty_acid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031259">
        <property type="entry name" value="ILBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000566">
        <property type="entry name" value="Lipocln_cytosolic_FA-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11955">
        <property type="entry name" value="FATTY ACID BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00061">
        <property type="entry name" value="Lipocalin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00178">
        <property type="entry name" value="FATTYACIDBP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50814">
        <property type="entry name" value="Lipocalins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lipocalin/cytosolic fatty-acid binding" evidence="2">
        <location>
            <begin position="10"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8044629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="141" mass="16249" checksum="652206D89335ADA4" modified="2023-09-13" version="1">MASNNQANFSGTWHLVKSHNLESFLKECGVGFMRRNLAKAVSPVVYITQDGDNFVIKTDTVHRSVTLDFSVDVKFKSFLPWEAEEVLMVATWEGDKLVIKTVERENGPVFSRYIEEDHLVLEQEMNSVTCKRYFKRKEVAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CJ84</accession>
    <name>A0A9Q1CJ84_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Structural maintenance of chromosomes protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HOLleu_05005</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Nanhai2018</strain>
            <tissue evidence="16">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SMC family. SMC1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CJ84"/>
    <dbReference type="OrthoDB" id="413649at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008278">
        <property type="term" value="C:cohesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007062">
        <property type="term" value="P:sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03275">
        <property type="entry name" value="ABC_SMC1_euk"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1060.20:FF:000001">
        <property type="entry name" value="Structural maintenance of chromosomes 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000564">
        <property type="entry name" value="Structural maintenance of chromosomes 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1620:FF:000001">
        <property type="entry name" value="Structural maintenance of chromosomes 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000562">
        <property type="entry name" value="Structural maintenance of chromosomes protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1060.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003395">
        <property type="entry name" value="RecF/RecN/SMC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024704">
        <property type="entry name" value="SMC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028468">
        <property type="entry name" value="Smc1_ABC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010935">
        <property type="entry name" value="SMC_hinge"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036277">
        <property type="entry name" value="SMC_hinge_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18937:SF12">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18937">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES SMC FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06470">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02463">
        <property type="entry name" value="SMC_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005719">
        <property type="entry name" value="SMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00968">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75553">
        <property type="entry name" value="Smc hinge domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="9 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0498">Mitosis</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SMC hinge" evidence="15">
        <location>
            <begin position="512"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="300"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="349"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="395"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="679"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="941"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="161"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="796"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="1043"/>
            <end position="1073"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="300"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="324"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR005719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ8046357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1245" mass="143095" checksum="B388F96EABD7A1DB" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CJJ4</accession>
    <name>A0A9Q1CJJ4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_08678</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CJJ4"/>
    <dbReference type="OrthoDB" id="10072198at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46670">
        <property type="entry name" value="ENDO/EXONUCLEASE/PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46670:SF3">
        <property type="entry name" value="ENDONUCLEASE_EXONUCLEASE_PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="2">
        <location>
            <begin position="281"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="606"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="619"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="656"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="683"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="726"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="744"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="775"/>
            <end position="786"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8045634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="786" mass="88274" checksum="D53D5EFEBD2055E5" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CJK4</accession>
    <name>A0A9Q1CJK4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Fasciculation and elongation protein zeta-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_08832</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the zygin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CJK4"/>
    <dbReference type="OrthoDB" id="7959977at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011680">
        <property type="entry name" value="FEZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12394:SF12">
        <property type="entry name" value="LD08195P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12394">
        <property type="entry name" value="ZYGIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07763">
        <property type="entry name" value="FEZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="251"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="19"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8045759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="417" mass="47065" checksum="F285092297B9A854" modified="2023-09-13" version="1">MPGEMKTTNMAAPIAVFEENSDPNPDEWPSEFIDYRSPTTMDDMLNSNGESFPVLSPKEEISPDNFCEMKTSSSIEDLVNNFEEKLAVCFRNYDFDTERFAPVQVLTKEELMRNSKLWKEMTEHMGQVMPLDWETSKTRELHLPSLNLSQRRVEDHSLDLPEDDDLAQSLDMHSMVLSGSTTTPVDEEDGEEYPVVTAEQVIEELDEMFQCETSPDEPPEEDRLSSMSRELQALRERSLATGNYEEGNVGQALKEMDLAQLNELLEELDNTFREFSEILVQQLALREEMEDEKEMKNMFIASLLSVQNKIKSKNGKGKGKSEGSSPGGLAGLGLISRIKGKNGLVEPGYYLTTRIPYNKGDGPPDLDTLRMLTKILRAIDVDSKEVPDLLTNYILKVLFPSGTEEGQTLPAPDLTQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CK01</accession>
    <name>A0A9Q1CK01_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA replication licensing factor MCM2</fullName>
            <ecNumber evidence="4">3.6.4.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">DNA replication licensing factor mcm2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">HOLleu_05744</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="22">Nanhai2018</strain>
            <tissue evidence="22">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MCM family.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="4"/>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CK01"/>
    <dbReference type="OrthoDB" id="844at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042555">
        <property type="term" value="C:MCM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000228">
        <property type="term" value="C:nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017116">
        <property type="term" value="F:single-stranded DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902975">
        <property type="term" value="P:mitotic DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17753">
        <property type="entry name" value="MCM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.10:FF:000002">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1640.10:FF:000005">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000138">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1640.10">
        <property type="entry name" value="mini-chromosome maintenance (MCM) complex, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008045">
        <property type="entry name" value="MCM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027925">
        <property type="entry name" value="MCM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059098">
        <property type="entry name" value="WHD_MCM2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630:SF44">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12619">
        <property type="entry name" value="MCM2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14551">
        <property type="entry name" value="MCM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23669">
        <property type="entry name" value="WHD_MCM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01658">
        <property type="entry name" value="MCMPROTEIN2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="17" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0235">DNA replication</keyword>
    <keyword evidence="15" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="12" id="KW-0347">Helicase</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <keyword evidence="10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="21">
        <location>
            <begin position="463"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="20">
        <location>
            <begin position="52"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="20">
        <location>
            <begin position="85"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="96"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00074927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KAJ8046907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="889" mass="100564" checksum="5829BBF7550EB1A0" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CK89</accession>
    <name>A0A9Q1CK89_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_05712</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CK89"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="133"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="149"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8046882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="281" mass="30859" checksum="E975C167A840020B" modified="2023-09-13" version="1">MLLQRDELFAILATMLHTFFHIVPVTVSKAVTHIPLPPASASSFARENCLPSLNEGLIQVNLTRTVNGDEFTRYCGLNCDQLCHSAGAVRLLCDIKEIPQCRCKCLNNETILSDSSTCKASLQGCQPPILHIHVPPIGDDSSSNTSSSRIDEREETQGDLSKRPTPLECRLLGVHSAGTDGRELFNISQSFNVTRIDEKRIEVTLTKVTHELRRLQGQLLKLNVNCQASLFCIAVKLSGSIKHSINFHSPHLPSAMTYDDDITTQEIGTMTPAAKGSFFSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CLE9</accession>
    <name>A0A9Q1CLE9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">EF-hand calcium-binding domain-containing protein 12</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_06391</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CLE9"/>
    <dbReference type="OrthoDB" id="10005811at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042847">
        <property type="entry name" value="EFC12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47225">
        <property type="entry name" value="EF-HAND CALCIUM-BINDING DOMAIN-CONTAINING PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47225:SF1">
        <property type="entry name" value="EF-HAND CALCIUM-BINDING DOMAIN-CONTAINING PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="208"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="244"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="286"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="356"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="362"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8047403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="628" mass="73322" checksum="E9EA86B06D31F277" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CM94</accession>
    <name>A0A9Q1CM94_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_06030</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CM94"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008191">
        <property type="term" value="F:metalloendopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002020">
        <property type="term" value="F:protease binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051045">
        <property type="term" value="P:negative regulation of membrane protein ectodomain proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001820">
        <property type="entry name" value="TIMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008993">
        <property type="entry name" value="TIMP-like_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11844">
        <property type="entry name" value="METALLOPROTEASE INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11844:SF33">
        <property type="entry name" value="TISSUE INHIBITOR OF METALLOPROTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00965">
        <property type="entry name" value="TIMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50242">
        <property type="entry name" value="TIMP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040279198" evidence="5">
        <location>
            <begin position="27"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared with metalloproteinase partner</note>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="27"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="29"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="41"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR601820-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR601820-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8047124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="186" mass="20428" checksum="5BAB235D71546FCF" modified="2023-09-13" version="1">MESLQKTCIFFFLNLVTFLLPSFVESCTCPPSTQSFEMNFCLADYSYKGTIVNVEVVSDEGTVSLYYDVIVDEVYHGMEVVDAGSRIEVTTGLSGSECGMPGLAKGIYLFQGKGSPKEVSFCDFVKPLAEVSSAVQDGLTSGDYSRICAYFYDNICLVTARPEVTMEETTRASRLVDMEFTTADSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CN19</accession>
    <name>A0A9Q1CN19_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transcriptional activator protein Pur-alpha</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_00052</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PUR DNA-binding protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8047931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CN19"/>
    <dbReference type="OrthoDB" id="523901at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032422">
        <property type="term" value="F:purine-rich negative regulatory element binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2450.30:FF:000001">
        <property type="entry name" value="Purine-rich element binding protein A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2450.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006628">
        <property type="entry name" value="PUR-bd_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12611">
        <property type="entry name" value="PUR-TRANSCRIPTIONAL ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12611:SF0">
        <property type="entry name" value="PURINE-RICH BINDING PROTEIN-ALPHA, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04845">
        <property type="entry name" value="PurA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00712">
        <property type="entry name" value="PUR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="262"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="27"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="279"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8047931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="290" mass="33123" checksum="A82FDB40497BF0FB" modified="2023-09-13" version="1">MSDRDSTEDQPQGQGADLGMDDSGSEPEMRSQEDEGEMRGDVQELATKTLHIQSKKYYLDVKQNRRGRFLKIAEVPGRGPKKRLTLRMPAAAEFRDYLTDFSEHYAQLGPANPDNIPEDGRLKSEQIISDNRRYYLDLKENTRGRFLKVSQTGDRGPRIQIAVPAQGLVEFKDALTELLEEFGTDDPSVEENQTPLPKSQQFWVENKHFFFDIGSNYRGVYMRISEVQPRSQYRTSITIPEKSWARFRDTFTDYCSKMEELKKSHPNGGAAEGHSRAEQEEEAVGEGEAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CP21</accession>
    <name>A0A9Q1CP21_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_00198</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CP21"/>
    <dbReference type="OrthoDB" id="5814287at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31751:SF42">
        <property type="entry name" value="PROTEIN CBG10204"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31751">
        <property type="entry name" value="SI:CH211-108C17.2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="220"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8048039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="245" mass="28501" checksum="39BEA88F2B62FFD1" modified="2023-09-13" version="1">MEKLGFQNCMKSFDESGLTSDVLATDRHVRIRKVLRAEFPDVDHEFDIWHLTKSVSKKLTTKTTFKECEELGPWINSVKNHLWRCAQNCNGNYEVLLEMWTSIVHHVNNVYHWNSAQYFYRCAHDQLSENDARSEKWIAPGSKAHKARSEVVFDKRLTKDLHVCYMCYTGSLEVFHCILLKYCPKRLHFSYPAMQARLQLAVLDHNNVGREQVVVTKENTKLDPECQNDGGIRIPEQPSNGCLDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CP81</accession>
    <name>A0A9Q1CP81_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phospholipid scramblase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_00883</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">May mediate accelerated ATP-independent bidirectional transbilayer migration of phospholipids upon binding calcium ions that results in a loss of phospholipid asymmetry in the plasma membrane.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the phospholipid scramblase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CP81"/>
    <dbReference type="OrthoDB" id="191150at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017128">
        <property type="term" value="F:phospholipid scramblase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005552">
        <property type="entry name" value="Scramblase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23248:SF63">
        <property type="entry name" value="PHOSPHOLIPID SCRAMBLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23248">
        <property type="entry name" value="PHOSPHOLIPID SCRAMBLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03803">
        <property type="entry name" value="Scramblase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0564">Palmitate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="22"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU363116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8048531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="119" mass="13356" checksum="2B280A8136963593" modified="2023-09-13" version="1">MDEKQQEQQTQYGMAGNVVTVPPAPGPPPGQALPGQVQIQWMAPPPQSILGCPPGMEYLTQLDQLLVHQQVELFEAFTGIDTKNRYQIKNALGQQVNSFDKVIIFSTRLVLHFCKECYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CTU8</accession>
    <name>A0A9Q1CTU8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_04282</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CTU8"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8050903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="437" mass="48510" checksum="75EDA2BE06915EAA" modified="2023-09-13" version="1">MGRGFSRKSRKHTFYPRLSSSKVLVTILPKSRAPVLVGPTSVHALPGGDQKLFSKRNFGNLLMGRGFSRRSRKYAFYPRLSSVKVLVTILLKSRAPVLVGLTSVHAPPGSDEKLFSKRNFGNLLMGRGFSRKSRKHTLYPRLSSAKVLVTILLKSRAPVLVGLTSVHGLPGSDQIFFSKRNFGNLLMGRGFSRKSRKHTLYPRLSRVKALVTILLKSRAPVLVGLTSVHAPPGSDQILFSKRNFGNLLMGRGFSRKSRKRTFYPRLSRVKALVTILLESIADVLVGLRSVHALPGSDQKLFSTRNFGNLLMGRGFSRKSRKHTPYPRLPFGYKGTGDQFTEEQALPVHVGPKSDECLPGGDRPKEFGKSFQGMRAVEKIEENKKSHFLLVTNFLIDRVPAVIGLKSLHHSRHPSVIVCFCPPFISPVPNTSVNLSAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H0U5</accession>
    <name>A0A9Q1H0U5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Vacuolar protein sorting-associated protein 45</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_28396</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the STXBP/unc-18/SEC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H0U5"/>
    <dbReference type="OrthoDB" id="10266265at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.830.10:FF:000002">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2060:FF:000003">
        <property type="entry name" value="vacuolar protein sorting-associated protein 45 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.830.10">
        <property type="entry name" value="Syntaxin Binding Protein 1, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043154">
        <property type="entry name" value="Sec-1-like_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043127">
        <property type="entry name" value="Sec-1-like_dom3a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001619">
        <property type="entry name" value="Sec1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027482">
        <property type="entry name" value="Sec1-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036045">
        <property type="entry name" value="Sec1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11679">
        <property type="entry name" value="VESICLE PROTEIN SORTING-ASSOCIATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00995">
        <property type="entry name" value="Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005715">
        <property type="entry name" value="VPS45_Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56815">
        <property type="entry name" value="Sec1/munc18-like (SM) proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00073001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8029083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="550" mass="62454" checksum="81C28BFB14AD0F5A" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H1E4</accession>
    <name>A0A9Q1H1E4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">FAST kinase domain-containing protein 5, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_28356</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H1E4"/>
    <dbReference type="OrthoDB" id="10064757at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035770">
        <property type="term" value="C:ribonucleoprotein granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000963">
        <property type="term" value="P:mitochondrial RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044528">
        <property type="term" value="P:regulation of mitochondrial mRNA stability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050870">
        <property type="entry name" value="FAST_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010622">
        <property type="entry name" value="FAST_Leu-rich"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21228">
        <property type="entry name" value="FAST LEU-RICH DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21228:SF40">
        <property type="entry name" value="LD45607P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06743">
        <property type="entry name" value="FAST_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FAST kinase leucine-rich" evidence="1">
        <location>
            <begin position="417"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8029055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="520" mass="60874" checksum="349CA13E7A2A2508" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H1H3</accession>
    <name>A0A9Q1H1H3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_29158</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H1H3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8029698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="136" mass="15487" checksum="A01795D275D3C730" modified="2023-09-13" version="1">MGTRVDPGLRPEELTPGIRFSHNRDQMNTWGPKQMKDQPAATGGYSRIGWYESGSNGQHNNPFFGYRDNEQMRYNHGRVSNIPHRPDLGSPWEDKVRAGYTESRPRYERLAGGEYNHPLEGYTEKGGMQHKSHGGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H2U4</accession>
    <name>A0A9Q1H2U4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Zinc finger HIT domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_27544</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H2U4"/>
    <dbReference type="OrthoDB" id="74807at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21437">
        <property type="entry name" value="zf-HIT_ZNHIT1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039723">
        <property type="entry name" value="Vps71/ZNHIT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007529">
        <property type="entry name" value="Znf_HIT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13093">
        <property type="entry name" value="ZINC FINGER HIT DOMAIN CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04438">
        <property type="entry name" value="zf-HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144232">
        <property type="entry name" value="HIT/MYND zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51083">
        <property type="entry name" value="ZF_HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="HIT-type" evidence="6">
        <location>
            <begin position="124"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="38"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="14"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8030973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="161" mass="18551" checksum="573C73992BC240D2" modified="2023-09-13" version="1">MVMAEAKTKQGSSRLKDANSRKILDQYSRAKRLRKQLEALEQDNFQEDPHIHLNTPNKKLPQFDADEGAPTKKKRKTTRGDHFKQRFRKTFASLVEEEQMSLKEPPNYMTACIPQSKFPARKFCSVCGNPSNYTCVTCGSRYCCVRCLGTHQDTRCLKWTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H2U5</accession>
    <name>A0A9Q1H2U5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Malignant fibrous histiocytoma-amplified sequence 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_23333</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H2U5"/>
    <dbReference type="OrthoDB" id="1394818at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF36">
        <property type="entry name" value="CASPASE FAMILY P20 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="4">
        <location>
            <begin position="70"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="252"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8033177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="300" mass="34214" checksum="8E2854EE8AC9B573" modified="2023-09-13" version="1">MNSSNSFKPRTMIHLRVFKDPTFQYKCLKLSGRELADIPPELFELYFLEFLVLSPERESGLTFRLLTVPKEIGELQQLRILCLDTNELKKLPNEIGRLKNLERLVLSNNLLTGLPESFSDLQNLKHLHLANNYFKSIPICVYSMLNLVFLDASDNKISRISQDIGALVCLESLLLLYNLLETLPDAITKCIHLKNLWLGNNRLKSLPKKFGDLTKVDWEFHPNSANIDGNPLQHPPVEVCRQGPDAIRDYFKQNETPPMDYEDGSSPEDHGFGDSVMSSSLHLSSDWLVVPPIDIDTPRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H2Y3</accession>
    <name>A0A9Q1H2Y3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_23468</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H2Y3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040193231" evidence="3">
        <location>
            <begin position="19"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="130"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="166"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="166"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="199"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8033282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="235" mass="26992" checksum="0E3B66D31D92ACD3" modified="2023-09-13" version="1">MLILHFLVWIQMSPLWRSKIIQVSYLVRRGGDSEGDQGDMPLSVEQEEVKDEKDELICQNNSSSTTYLDKGSVKKLNQSLSNLKSGKIQVQGINLTISYRLKIVSDHFRRRRFTICLTNVEFKQQTNLPIWLLSVIISIVILVCLAGVGVVIRQRKLQVHQKRAKVDVEKEDTQSRQTKEHSTSVKEDTCHPSFGQDLLNDKDEDDHPYCEIDIKQPPTNNLIYNSAYESVSSFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H486</accession>
    <name>A0A9Q1H486_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Intraflagellar transport protein 46 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_21793</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium basal body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IFT46 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H486"/>
    <dbReference type="OrthoDB" id="2119217at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030992">
        <property type="term" value="C:intraciliary transport particle B"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005815">
        <property type="term" value="C:microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042073">
        <property type="term" value="P:intraciliary transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022088">
        <property type="entry name" value="Intraflagellar_transp_cmplxB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13376">
        <property type="entry name" value="INTRAFLAGELLAR TRANSPORT PROTEIN 46 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13376:SF0">
        <property type="entry name" value="INTRAFLAGELLAR TRANSPORT PROTEIN 46 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12317">
        <property type="entry name" value="IFT46_B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0969">Cilium</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="95"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="374"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="10"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="22"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="58"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="95"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="105"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8034797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="398" mass="44322" checksum="6984FE097D3FC97F" modified="2023-09-13" version="1">MADDSEGETPKTQLVSNQPYDESLDVPDAEEVASVYTPSPRLPPNQGSDCKQGGGQSGSLAQDSMTDPSDDDLETGVMENKNFVLFVKPIMQPLQQVKQRQNAESVEDDDEDDDEDDDDDDDGSDSSDDDDDDDNQGRGLEGAYDPAEYENLPVSSEIKELFQYITRYTPQTIELEHKLKPFIPDYIPAVGDIDAFIKINRPDGQTDTLGLTVLDEPCAKQSDPTVLDLQLRAISKQTTSKAMQVRSIENASKQPKLINNWVESISELHRSKPPPTVHYTKTMPEIDTLMQEWPPEFEDLLNKIGVPTADMDCTLDQYVEMICAILDIPIYQSKIQALHVLFTLFSEFKNSQYFKTLAEENKLDNKLKVGQAGNEEKVGEEGDDFAAGGGDFNDDLGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H6K3</accession>
    <name>A0A9Q1H6K3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SCAN box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_21082</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H6K3"/>
    <dbReference type="OrthoDB" id="6077919at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF1">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50804">
        <property type="entry name" value="SCAN_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCAN box" evidence="2">
        <location>
            <begin position="169"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="254"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="261"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8034305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="273" mass="31499" checksum="5840117379DA82AB" modified="2023-09-13" version="1">MADTDGPSEGQQQDSSSGTWEVHQSSEDALPQGIPPDLPKEMWVQYLCECKRIEKEAELNLKRLELERDHIAQSNTRPMGGLDHYQGARHSRVPRLKATDDIDIYFKTFENLAKIYGWPEAEWATRLAPELTGKARAAYASLSLEDCCDYSKLKKVVLAKYEISAESYRVAFRSKYKRRDQGLREWISDLGHQFDRWLEYAEVKKDDAKGIRELMVIDQALSKLPSEMAIYLKDRNPRTSEDLTKMADQFVANRGGPNYWKRKEADQAAKKQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H6K4</accession>
    <name>A0A9Q1H6K4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phorbol-ester/DAG-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_18284</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H6K4"/>
    <dbReference type="OrthoDB" id="6750291at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15517">
        <property type="entry name" value="PHD_TCF19_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050863">
        <property type="entry name" value="CenT-Element_Derived"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004875">
        <property type="entry name" value="DDE_SF_endonuclease_dom"/>
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    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007889">
        <property type="entry name" value="HTH_Psq"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19303:SF74">
        <property type="entry name" value="POGO TRANSPOSABLE ELEMENT WITH KRAB DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19303">
        <property type="entry name" value="TRANSPOSON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03184">
        <property type="entry name" value="DDE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05225">
        <property type="entry name" value="HTH_psq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="4">
        <location>
            <begin position="276"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8037467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="335" mass="37778" checksum="6A629764F6D23A36" modified="2023-09-13" version="1">MGRLRAWVSILGDAVPLQRKTTRASWSPDIMRHAVEVVRRGEMGVKRAAKLYSVPVSTLRDKVRSNSNEFTPKLGRNPTLGREFEADLVKYLVKMEARGFGLTRADIRRLTFQFSERNGIAHTFNNNMAMPRKKGETVTVVGCCSASGLYIPPYVVMKGKRMKDEYSDGLPPGSKLSLSDTGYINSDLFLDWLKHFRSFACPGKVLLLVDGHASHAKSVAVLDYARDKDIIMFCLPPHTTKYLQPLDRSVYKPLKVFYQHATHKFVRSQPGSTISKYRFGSLFSEAWGKTATYCGLCSGFFYNGDGEEWVECSECSVWFHVDCMDMVGGRCDDCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H783</accession>
    <name>A0A9Q1H783_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Influenza virus NS1A-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_15565</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H783"/>
    <dbReference type="OrthoDB" id="45365at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="CDD" id="cd18306">
        <property type="entry name" value="BTB_POZ_NS1BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017096">
        <property type="entry name" value="BTB-kelch_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011043">
        <property type="entry name" value="Gal_Oxase/kelch_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632:SF26">
        <property type="entry name" value="BTB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632">
        <property type="entry name" value="LD33804P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037037">
        <property type="entry name" value="Kelch-like_protein_gigaxonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50965">
        <property type="entry name" value="Galactose oxidase, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BTB" evidence="4">
        <location>
            <begin position="48"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="666"/>
            <end position="695"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8038217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="695" mass="77887" checksum="ED5A2A6A59D83035" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H809</accession>
    <name>A0A9Q1H809_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Zinc finger protein Gfi-1b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_19574</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the histone deacetylase HD2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8035791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H809"/>
    <dbReference type="OrthoDB" id="654211at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000184">
        <property type="entry name" value="Zinc finger protein 333"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.340">
        <property type="entry name" value="Nucleoplasmin core domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041232">
        <property type="entry name" value="NPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050331">
        <property type="entry name" value="Zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515:SF49">
        <property type="entry name" value="GASTRULA ZINC FINGER PROTEIN XLCGF49.1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17800">
        <property type="entry name" value="NPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="414"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="447"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="475"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="503"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="164"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="186"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="235"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8035791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="537" mass="61063" checksum="BE8A526BA34D2AD2" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HBW4</accession>
    <name>A0A9Q1HBW4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">HOLleu_13886</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="22">Nanhai2018</strain>
            <tissue evidence="22">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein-tyrosine phosphatase family. Receptor class 2A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="3"/>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8039778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10253954at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14553">
        <property type="entry name" value="R-PTPc-LAR-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000028">
        <property type="entry name" value="Neuronal cell adhesion molecule"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000032">
        <property type="entry name" value="palladin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000551">
        <property type="entry name" value="Protogenin A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000010">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase delta isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000027">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase delta isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000036">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase delta isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000068">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase delta isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000023">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase delta isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000002">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase delta isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000001">
        <property type="entry name" value="Receptor-type tyrosine-protein phosphatase F isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050713">
        <property type="entry name" value="RTP_Phos/Ushers"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46957">
        <property type="entry name" value="CYTOKINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46957:SF6">
        <property type="entry name" value="PROTEIN-TYROSINE-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00014">
        <property type="entry name" value="FNTYPEIII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="10 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1370"/>
            <end position="1395"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="5"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="106"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="208"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="21">
        <location>
            <begin position="298"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="21">
        <location>
            <begin position="393"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="21">
        <location>
            <begin position="493"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="21">
        <location>
            <begin position="588"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="21">
        <location>
            <begin position="695"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="21">
        <location>
            <begin position="805"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="21">
        <location>
            <begin position="900"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="21">
        <location>
            <begin position="998"/>
            <end position="1095"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="21">
        <location>
            <begin position="1096"/>
            <end position="1197"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="18">
        <location>
            <begin position="1460"/>
            <end position="1717"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="19">
        <location>
            <begin position="1637"/>
            <end position="1708"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="18">
        <location>
            <begin position="1749"/>
            <end position="2008"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="19">
        <location>
            <begin position="1923"/>
            <end position="1999"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="374"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="377"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KAJ8039778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="2016" mass="224743" checksum="4BB65672E2F34273" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HC59</accession>
    <name>A0A9Q1HC59_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sulfotransferase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_11168</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HC59"/>
    <dbReference type="OrthoDB" id="416710at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48312">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48312:SF1">
        <property type="entry name" value="SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8043872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="320" mass="37713" checksum="ED0182B1B3E4CA72" modified="2023-09-13" version="1">MTSSSQKPKLIFLWIVPRTCSTALSKCMTFVDDTEVWAEPYMACHFNETFYNPNFRKGDTVADRMRETLKKNEASETMRAIRKEMATKIEKSPNLIDQKDISYQWLKEQLEKSDSEKKFIFVKDQSLAINNHFEYLPDVPTHHTFMIRHPREIYPSYKSMTWKRLDFDGTKTWDEWHAADDSPFLPVRDLFQIHLKIWKHFRETSDNEPIIIDGYDLASKPDVILPKYFERLGIPFKKSYLKWDSSENIIEKKWKGSADMVLMEPKTVVYARAVKSSAFEPPKVPRGTPSNPEWTITPELEEMIDGALPFYEEMFQARLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HC75</accession>
    <name>A0A9Q1HC75_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_11257</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HC75"/>
    <dbReference type="OrthoDB" id="6125049at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000003">
        <property type="entry name" value="Protein CBG25708"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF11">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="1">
        <location>
            <begin position="310"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8043932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="476" mass="52848" checksum="EA0E63ECDC324DCD" modified="2023-09-13" version="1">MVKQVSEGEIDYEFTTTASKLNSLPSCQVEIAGVKIKMGIDSQASVNVLDKLAFQKLKDKAGAPENCLRPCSTALFAYNSKKPLPTIGEIRARVTTDTGKTAKTCFCIIDGIGGNLLCYQTSVDLGILHIVNSVESRVFAQYPELFKGIGKINTDPIKLHTDTDVEPKAQKLRPIPFKYVCSNAIPKAMKFELVQSESKRDSEIQAIKIALTTGNWSDESVRQCRNLKNEFSVFKGVVLRDTHIVLPQSLREHAVDLAHASHQGIVKTKQLLREKVWFPGIDNSIERKVKGCLTCQIATPTPTPPEPLQMTELPNASWESVSMDFKGPFMGGEYIMVVADDYSRYPEAVVVTSLQAKTVIPELDKIFSRHRIPKVVRTNNGPPMNSEDFSKFANYLGFTQRKCTPLWPHANGEAERFIRTLVKSIRASVLREIESKNCANFCANTELHLTALRVCLCSSLCMAGKCLSRSQELNAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HCQ3</accession>
    <name>A0A9Q1HCQ3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_14865</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HCQ3"/>
    <dbReference type="OrthoDB" id="10059413at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1820">
        <property type="entry name" value="L1 transposable element, RRM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004244">
        <property type="entry name" value="Transposase_22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11505">
        <property type="entry name" value="L1 TRANSPOSABLE ELEMENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="60"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="24"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8040543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="257" mass="29702" checksum="5416159AC4B18C05" modified="2023-09-13" version="1">MADARPRGRPPGSKNKKKDELPLSQASVTPFQLRPSRSLTQLPALEYESSEGDVAVSTGLQSLDLAIQNITTELQNIRSEFAKSIEEHAKLISSLQEKNVTLAKKVDELEKRQKDQEQLLNKQERHSRRNNFRIVGIKQEKDEDCLKIASGILCKMGFPDPKLERAHRDGRAVEGRDRHILVKLSFFQDKVKVMKNARRLLEGENFYITDDLTKKDLSEKRRWRDQVSDLYRQGTRLHFSGGYWRDSTGKPFNFHQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HCQ7</accession>
    <name>A0A9Q1HCQ7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable RNA polymerase II nuclear localization protein SLC7A6OS</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_12079</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Directs RNA polymerase II nuclear import.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the IWR1/SLC7A6OS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HCQ7"/>
    <dbReference type="OrthoDB" id="6255506at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032502">
        <property type="term" value="P:developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040218">
        <property type="entry name" value="SLC7A6OS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013883">
        <property type="entry name" value="TF_Iwr1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31196">
        <property type="entry name" value="RNA POLYMERASE II NUCLEAR LOCALIZATION PROTEIN SLC7A6OS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31196:SF2">
        <property type="entry name" value="RNA POLYMERASE II NUCLEAR LOCALIZATION PROTEIN SLC7A6OS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08574">
        <property type="entry name" value="Iwr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Transcription factor Iwr1" evidence="11">
        <location>
            <begin position="232"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="28"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="81"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="140"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="181"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="277"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="41"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="100"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="115"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="146"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="189"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="286"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8041294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="348" mass="39051" checksum="F2C54FF425159D7F" modified="2023-09-13" version="1">MMSTVNVIRVRRKRTQDPLENLVLPLKRTKVNKPTGGTGSQGSAASATGQDDNKAVFTFAGTVERKNQPISVHVQSALRRASIGLQPKKPVRQSQQSVKAKTESNRTGAREKARSSHKAASKNRRLKVVESARGLKLHELDKDAQRFQSPSRRPLSNGSGALRSGLAGNDPERLFTLVDVEHEEEVKKSTPTKSKTPASKSKKSGSDDITCNSVKMTREKVVIGEDVIQESEYVYDLYYTDCSEFDFGDTGRTIGLRAEKEDLVYEYFADNQDLVYDDEDDENDESNWRNDYPDEEEHRPDHDMFDELVDDYNYASSDEDDLVAQYSKFKLGSKGGDDSDSDLDDYDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HDE0</accession>
    <name>A0A9Q1HDE0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_13029</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8042059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HDE0"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8042059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="52" mass="5987" checksum="8E320AE58DFE2D26" modified="2023-09-13" version="1">MRCGERKNPIVFGGGQRSSGVTGGQNLKTLFTRYLKLESLDRFYAWYVDALW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HFJ5</accession>
    <name>A0A9Q1HFJ5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Tripartite motif-containing protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_07330</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HFJ5"/>
    <dbReference type="OrthoDB" id="6105938at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.80">
        <property type="entry name" value="Phosphotransferase system, lactose/cellobiose-type IIA subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017868">
        <property type="entry name" value="Filamin/ABP280_repeat-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001258">
        <property type="entry name" value="NHL_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047153">
        <property type="entry name" value="TRIM45/56/19-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015063">
        <property type="entry name" value="USP8_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25462">
        <property type="entry name" value="BONUS, ISOFORM C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25462:SF296">
        <property type="entry name" value="MEIOTIC P26, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00630">
        <property type="entry name" value="Filamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08969">
        <property type="entry name" value="USP8_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101898">
        <property type="entry name" value="NHL repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140856">
        <property type="entry name" value="USP8 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50194">
        <property type="entry name" value="FILAMIN_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51125">
        <property type="entry name" value="NHL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="9">
        <location>
            <begin position="319"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="5">
        <location>
            <begin position="470"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="repeat" description="NHL" evidence="7">
        <location>
            <begin position="616"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="219"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="280"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="18"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="36"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="54"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="219"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="243"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="280"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8044549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="805" mass="87287" checksum="7A9903E0CABA8946" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HH66</accession>
    <name>A0A9Q1HH66_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_05088</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HH66"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040472066" evidence="1">
        <location>
            <begin position="25"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8046424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="224" mass="25385" checksum="EFBEBC4EDDBD9EDE" modified="2023-09-13" version="1">MQVLEMTSLLKELIILILVSRIIPVVLDRGSESEESEDRAKGQGRNIRGRSRKFFQIHGDELVFQPQHQHGCAKFLRKVQKIIGQSSTIHIRDVGTFNLSNVDCSQQRCEIKPRRQLCGSNKKDKTSLRIFKNACHQCAEGCKQVKKGSNPFERVKNAFIVNGNCYNYPPVLVREEPDTVFCSRPNSDHSCSLRCSDLQLPLSGPQDLPHLPNFRSSCAEGHNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HHC4</accession>
    <name>A0A9Q1HHC4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Leucine-rich repeat-containing G-protein coupled receptor 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_02919</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HHC4"/>
    <dbReference type="OrthoDB" id="676979at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366">
        <property type="entry name" value="IG(IMMUNOGLOBULIN) AND LRR(LEUCINE RICH REPEAT) DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366:SF96">
        <property type="entry name" value="LEUCINE RICH REPEAT CONTAINING 53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0675">Receptor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040228998" evidence="4">
        <location>
            <begin position="24"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="456"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8049947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="483" mass="54732" checksum="5A8FE23BEDBE9D08" modified="2023-09-13" version="1">MSALQKLLILILTFDSITNPCSIYFDTLNCDGRKSNVSFSSIFEVVHDRIEVTTLVIAYTNLSNLQNGNISLPYFPGLSRLRLYSNGLNVFCKYVISNNTAIKELSLQNNKLTAVPVTALEVLANLTYLDLSRNEITVIERFAFRFSTSLQYLNVASNRVHIIQRHSLSSLVNLELLSFANNSIQCRYFFISEFSSLRNLKTLNLAHNNINLTMKANASFSFPAHVLYLSYNALHFLDDFALNDSTNLRVLFADNNNISGISPLLFGKDSINLTTLVLDHNELMTIPVFLIQRLPNLQTLSLSWNRVTVVPNKAFQQNTHLLSIRLSWNQIHIVQEMSLQRLISLKQLDLRFNNLTSLPPRLFDSVGDFSLLIGGNNFTCDCEIYFLQKWIKKTRFFVDDIVCLISEFENFTSLISFEFSDTCPEATSSVTVLTDFPTNVTPSPPTSKVGFNFLKYWKGVAAVSMFIAVLFSVVALKLIPKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HJ90</accession>
    <name>A0A9Q1HJ90_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_01461</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HJ90"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8048944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="77" mass="9057" checksum="4316F01BF89F02E7" modified="2023-09-13" version="1">MANQWNTRLSVKCNCYGTIVIDIQHKYVRVLSLATLSALFIHLFFIVLGELFVRYLILFCFCLSFFFFFPFSDTIEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HJ99</accession>
    <name>A0A9Q1HJ99_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_06888</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HJ99"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8047790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="113" mass="12891" checksum="2598584DE9946D8E" modified="2023-09-13" version="1">MSSNSKMESLIPEVPSLPVYKDDSDLESSPKARQESCKGEHLKNDTRIRLEDHSYKRVKHHLAPLTKIQSTVISLTNEIKNWTAIWPKEESRLISEFQDPSPSYVVTSPALRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HK18</accession>
    <name>A0A9Q1HK18_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transcriptional coactivator p15 (PC4) C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_02996</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transcriptional coactivator PC4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HK18"/>
    <dbReference type="OrthoDB" id="2505440at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060261">
        <property type="term" value="P:positive regulation of transcription initiation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.31.10">
        <property type="entry name" value="Transcriptional Coactivator Pc4, Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003173">
        <property type="entry name" value="PC4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009044">
        <property type="entry name" value="ssDNA-bd_transcriptional_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045125">
        <property type="entry name" value="Sub1/Tcp4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13215">
        <property type="entry name" value="RNA POLYMERASE II TRANSCRIPTIONAL COACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02229">
        <property type="entry name" value="PC4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54447">
        <property type="entry name" value="ssDNA-binding transcriptional regulator domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcriptional coactivator p15 (PC4) C-terminal" evidence="8">
        <location>
            <begin position="113"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="Transcriptional coactivator p15 (PC4) C-terminal" evidence="8">
        <location>
            <begin position="214"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="32"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8049994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="280" mass="32301" checksum="5147B7347088AF81" modified="2023-09-13" version="1">MSTVIKTKIRELRRLNAVPLDLGVRKRIKLEEEETDSAVPLTHVDEIPQPSWPNHEGSSEDAVPPTPSDENAQPSWPNREGSSEASSMMNVSPLEENRETGTDTVSKPQVLFTLEENRKVSVKTFRNREYVCVREFYQVPESKYWHAGAKGLNLNVLEWDRLKLVASNMNYIISSSKDLFLSDEMRRFSLQPDYSDDDEFTGYCRKVKMMYFCTFKGTPYVSLRVYTQYSSGGQLYPTGRGIHLTLFQWEKLQELFQSIDNALEKLNDASMEERDSDKEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HKL7</accession>
    <name>A0A9Q1HKL7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein NLRC5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_00891</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HKL7"/>
    <dbReference type="OrthoDB" id="120976at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312">
        <property type="entry name" value="NACHT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312:SF2">
        <property type="entry name" value="NUCLEOTIDE-BINDING OLIGOMERIZATION DOMAIN-CONTAINING PROTEIN 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040333815" evidence="2">
        <location>
            <begin position="23"/>
            <end position="1145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="473"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="domain" description="NACHT" evidence="3">
        <location>
            <begin position="581"/>
            <end position="731"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8048538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1145" mass="129743" checksum="B91E0DB3488993BA" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HL95</accession>
    <name>A0A9Q1HL95_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_02149</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HL95"/>
    <dbReference type="OrthoDB" id="9909555at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21301">
        <property type="entry name" value="REVERSE TRANSCRIPTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21301:SF10">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8049408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="226" mass="25882" checksum="0B7BA859FFCB5DBE" modified="2023-09-13" version="1">MMDLTNKKVYRNNEYDKTTETVEHVNRIVATMLKEGDIDSSTAEYLTSREVKTSRYYTPPKSHKDKDEDGNLKTRPDISGNVSPIERLSEFVDFHINPEMRKLPSFVKDSKDILLKINSINNKGPLTIHWAMFTIDVKAMYPSMPQRLGLEGVRRALEARDTRVPSTNNLIECLKNCLNENHFEFKGKFFTQINGTSIGPKMPRLCMFGYGIGGRKTMGDMSVKTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HLV2</accession>
    <name>A0A9Q1HLV2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_04404</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8051000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HLV2"/>
    <dbReference type="OrthoDB" id="306876at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8051000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="111" mass="12876" checksum="9B4597ABC0E026AF" modified="2023-09-13" version="1">MFCNSTTAVSISLVITLILQRWRWPTVHEMVYVMLAGVSYASGHKTMFYALKVETATVVYCHTHLRYHLNFCVIGNISPFAAILDIVHWRYFDFYGLHWDHCKEQTTTVTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0Y8T4</accession>
    <name>A0A9Q0Y8T4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">GA-binding protein alpha chain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_44539</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000976">
        <property type="protein sequence ID" value="KAJ8017813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0Y8T4"/>
    <dbReference type="OrthoDB" id="10067219at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024668">
        <property type="entry name" value="GABP_asu_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11620">
        <property type="entry name" value="GABP-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GA-binding protein alpha subunit N-terminal" evidence="2">
        <location>
            <begin position="42"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8017813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="118" mass="12897" checksum="A97F73F9B98FEC4E" modified="2023-09-13" version="1">MEESKATSDLESQNTKQEEEIEDESEMESVSVVVGTPATQNVVTQMMDISETLGTLRTLLSQRLDCSLADHEIHLQNTTVLDADKSLLEQGVKAEGIVQFSVQVISSPGMLLLCSENI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YAB7</accession>
    <name>A0A9Q0YAB7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Fibronectin type-III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_44956</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010001506">
        <property type="protein sequence ID" value="KAJ8017550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YAB7"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26391">
        <property type="entry name" value="INACTIVE TYROSINE-PROTEIN KINASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26391:SF18">
        <property type="entry name" value="PROTEIN KINASE RECEPTOR TIE-1, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="2">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="166"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8017550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="191" mass="21735" checksum="A5669B12C051D4FA" modified="2023-09-13" version="1">MLTSPTPVSSSSTTITIRWSAWNETHDRGTPPVVSYNLYHKLSNNSDWKEAIPYDSVDGQDEYYATVKNLSPDTLYDFSVAAVREGYGGEGPKSPPLELQRTKSFEDLEEYLSIEEDYVKRPQNHENAGFRAPHVYDYAEEVHINRAGTSHQDVKLQQQYVKYENVKEKPTDSSGHVIPTYSTSLEDPLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YAK1</accession>
    <name>A0A9Q0YAK1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_44659</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010001084">
        <property type="protein sequence ID" value="KAJ8017730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YAK1"/>
    <dbReference type="OrthoDB" id="6279146at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38681:SF1">
        <property type="entry name" value="RETROVIRUS-RELATED POL POLYPROTEIN FROM TRANSPOSON 412-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38681">
        <property type="entry name" value="RETROVIRUS-RELATED POL POLYPROTEIN FROM TRANSPOSON 412-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8017730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="94" mass="10693" checksum="87B208F419DE42C2" modified="2023-09-13" version="1">MFWIRTDAVRKPLQPHYQGPYKVIDRQEKFYTLDINGSKNTVRIDRLKPANVQGMSTQPEDTPIPQPKPTPTSPATTSAAILLRPQKPQEPNLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YAW3</accession>
    <name>A0A9Q0YAW3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NLR family CARD domain-containing protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_42081</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000028">
        <property type="protein sequence ID" value="KAJ8019373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YAW3"/>
    <dbReference type="OrthoDB" id="120976at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312">
        <property type="entry name" value="NACHT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312:SF2">
        <property type="entry name" value="NUCLEOTIDE-BINDING OLIGOMERIZATION DOMAIN-CONTAINING PROTEIN 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="445"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="domain" description="NACHT" evidence="2">
        <location>
            <begin position="569"/>
            <end position="690"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8019373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1143" mass="131171" checksum="F715DD1996A78C0E" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YB11</accession>
    <name>A0A9Q0YB11_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_43089</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000205">
        <property type="protein sequence ID" value="KAJ8018745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YB11"/>
    <dbReference type="OrthoDB" id="8912020at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002218">
        <property type="term" value="P:activation of innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042509">
        <property type="entry name" value="ZCCHC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22639">
        <property type="entry name" value="GAG-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22639:SF3">
        <property type="entry name" value="ZINC FINGER CCHC DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="209"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="276"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="293"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="328"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="359"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8018745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="375" mass="41303" checksum="2E7231FC8489325B" modified="2023-09-13" version="1">MGSEKPGGSGTNSISMKEKSVRVFDDGFTSKELLEAVVEDIDVDGIVGCVKVGGQWVITLKDRQDADLLMETGLQIAGRPFAVQGVTKNIITVSLFGVPSYVTDDELNIKLREYGCIMKSAWTHKTFPEFPTIENGTRFVRLELPNDKKSLPYAIIINGVHLRLKHNGQVLVCNLCLADDHIMKNCPSYKCRECNNQGHSESRCPTVLCYACKKRGHKSFACPENDRQRNLVVEMDTDNARPDVNTEMNTGHSESPVTSIVECAHSVGLSNTVVKETLSSPPKNKDENPSKPPDSSTTSLSNSKDTSEDHPTKRSLSCDESEEDKESSQSSQSEASQSSKQLFRPKKHITPNLLMARNFTPNKQANDGSSSANTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YBJ7</accession>
    <name>A0A9Q0YBJ7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_43477</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000322">
        <property type="protein sequence ID" value="KAJ8018504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YBJ7"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8018504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="57" mass="6513" checksum="C0C51152BCFBC312" modified="2023-09-13" version="1">MKKCGENLYKWPGADDISWEARENLVCKPDFPEPANKRGQFKFSNLSFTNVADNMAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YBM0</accession>
    <name>A0A9Q0YBM0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Zinc finger protein Gfi-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_41253</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8019603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YBM0"/>
    <dbReference type="OrthoDB" id="10018191at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000100">
        <property type="entry name" value="Zinc finger 45-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000193">
        <property type="entry name" value="Zinc finger protein 300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008598">
        <property type="entry name" value="Di19_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394:SF29">
        <property type="entry name" value="MYONEURIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05605">
        <property type="entry name" value="zf-Di19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="978"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="1006"/>
            <end position="1033"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="1034"/>
            <end position="1057"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="256"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="391"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="547"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="773"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="824"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="15"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="267"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="291"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="330"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="391"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="592"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="632"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="828"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="842"/>
            <end position="858"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8019603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1083" mass="121572" checksum="B5AD27A871D6F8FE" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YBX1</accession>
    <name>A0A9Q0YBX1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_43273</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000254">
        <property type="protein sequence ID" value="KAJ8018639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YBX1"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8018639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="87" mass="10185" checksum="96C84E21E9B6193A" modified="2023-09-13" version="1">MCYKTTRQEHRIESIDERMESSREFNFNAAFLELSRILRLLSSIIASLVVIRSWNQFLRYSTLQVYYCTIVNVHALPALGTDQANIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YD59</accession>
    <name>A0A9Q0YD59_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Fucosyltransferase</fullName>
            <ecNumber evidence="11">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_41943</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Endomembrane system</location>
            <topology evidence="10">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Golgi apparatus</location>
            <location evidence="11">Golgi stack membrane</location>
            <topology evidence="11">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the glycosyltransferase 10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="11"/>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8020095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YD59"/>
    <dbReference type="OrthoDB" id="427096at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046920">
        <property type="term" value="F:alpha-(1-&gt;3)-fucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11660:FF:000002">
        <property type="entry name" value="Alpha-(1,3)-fucosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11660">
        <property type="entry name" value="Glycosyl transferase family 10, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055270">
        <property type="entry name" value="Glyco_tran_10_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031481">
        <property type="entry name" value="Glyco_tran_10_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001503">
        <property type="entry name" value="Glyco_trans_10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038577">
        <property type="entry name" value="GT10-like_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11929">
        <property type="entry name" value="ALPHA- 1,3 -FUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11929:SF145">
        <property type="entry name" value="ALPHA-(1,3)-FUCOSYLTRANSFERASE FUT-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17039">
        <property type="entry name" value="Glyco_tran_10_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00852">
        <property type="entry name" value="Glyco_transf_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="Fucosyltransferase N-terminal" evidence="13">
        <location>
            <begin position="96"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="Fucosyltransferase C-terminal" evidence="12">
        <location>
            <begin position="211"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00060399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF17039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ8020095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="419" mass="49225" checksum="09B069A447D1B060" modified="2023-09-13" version="1">MQLIRRNNLIRLILALGIVIVVKCLLMQPYTTEENRVKRFVGRLRMDIKLIGIEGKTRNCYKTFLIYGMKPYWKEFLTYKFISKYKYLIDFPRDISCPHLNCHVRLKFSNQAKDLAGVDVVVFTNTNEWLSPDMWEWTINNRTDGQKWIFISIESPLYASGLRPPDDYADLAYDWFASYKLDSDIYQPYGFYDVYEQHARPQHIDLQKFIAHKTHSVVWMGKNCDIFPWNREAFIESLSNLVNVDKFGACGDKELPWHNEEAVREVLSKYKFYLALENSCCVDYITEKFWRSLDFGLVPVVVGASYEQYLKVAPPNSFIHVDQFESIEDLAIYLVSVSNDDEKYLEFHKWRNLGGVFSYNQDEKFISPLMDSTQCAILEKVLTTSEEENKKLDFLGSKWRGSCGFCGYKWIQSFGHKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YEH0</accession>
    <name>A0A9Q0YEH0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NLR family CARD domain-containing protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_42824</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000142">
        <property type="protein sequence ID" value="KAJ8018914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="427518at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312">
        <property type="entry name" value="NACHT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312:SF2">
        <property type="entry name" value="NUCLEOTIDE-BINDING OLIGOMERIZATION DOMAIN-CONTAINING PROTEIN 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="615"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="domain" description="NACHT" evidence="2">
        <location>
            <begin position="744"/>
            <end position="867"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8018914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1303" mass="148305" checksum="5D0689A4724CB0FE" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YEW2</accession>
    <name>A0A9Q0YEW2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_39766</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YEW2"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8020231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="67" mass="7963" checksum="DF50954A7B5D5DBC" modified="2023-09-13" version="1">MERNYRRKQLYSMRSRLKIWVSKKLSYWVTGSCQDDILGHTELFSPTKFEKFPVKGKVAWKPSPGIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YFP9</accession>
    <name>A0A9Q0YFP9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Galactosylceramide sulfotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_40372</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the galactose-3-O-sulfotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YFP9"/>
    <dbReference type="OrthoDB" id="514299at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001733">
        <property type="term" value="F:galactosylceramide sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009247">
        <property type="term" value="P:glycolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009729">
        <property type="entry name" value="Gal-3-0_sulfotransfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14647">
        <property type="entry name" value="GALACTOSE-3-O-SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14647:SF85">
        <property type="entry name" value="GALACTOSYLCERAMIDE SULFOTRANSFERASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06990">
        <property type="entry name" value="Gal-3-0_sulfotr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040391173" evidence="10">
        <location>
            <begin position="22"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8020711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="425" mass="49799" checksum="869A85C70F78705B" modified="2023-09-13" version="1">MLHKIMKNSFLVLLLICTVFAILGFYGERRFKMKRSLPHYYRITSGKNTGTMRYDEGRIVSTAYHGNQKKVKGILQPPINISIGIECHETSNFVFIKTHKTGSTTLRSLTCRFGYFRNLSFLLGKGGVIGHLNNQAVKWSPNSSNLLPPIGVSPSDHANHRNYNISNIHWLFRRNIVRKLMYPASDLKYFTIIREPTEQWLSQFQYFKDYKRLGLKLETLSNTLLPFLLNLTEQPRKPSFNRQLRDLGVPSSVHKNATKLNEALSNIDKEFDLVLITEYFDESLILLKKLLCWRFEDILYVKKRQQPNPLTVDETTRREIRKYNSGDVTLYHYFLAVFWRKVKEYGPAFEEDLRTFRKLLNDTWNICIGNSVTEVSPDRYLYVETYLAQNSSEFCWALVNTKFQMDIEIVKRQGHTSDKRWAAFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YH09</accession>
    <name>A0A9Q0YH09_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_37353</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YH09"/>
    <dbReference type="OrthoDB" id="5577209at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="107"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8022453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="168" mass="20327" checksum="B2C94B2C2A808015" modified="2023-09-13" version="1">MESSREFNFNAAFLELSRNKFVASPSVQELDSLKKSEIVKVAKYYGIEFQPLMRKDEIKRYVLEYLVDESILPITVLETAITVPTDNTFELKRLEIEMNKEIRLKEMEREREKEEREMQMQKEKEEREMQMQKEKEEREMQMQREKEAREHEFRLKQLELGVIKASDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YJ17</accession>
    <name>A0A9Q0YJ17_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_35826</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YJ17"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8023393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="52" mass="5772" checksum="D0C9CCF8CEA7BE4D" modified="2023-09-13" version="1">MRYGMRKNPIIFGGGERSSGVAGGQTLKTLLTRYLKKGNSDSSQTWYIDAPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YJW0</accession>
    <name>A0A9Q0YJW0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">FAD synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_36215</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YJW0"/>
    <dbReference type="OrthoDB" id="270728at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003919">
        <property type="term" value="F:FMN adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006747">
        <property type="term" value="P:FAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="entry name" value="MoaB/Mog-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056596">
        <property type="entry name" value="FLAD1_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23293:SF9">
        <property type="entry name" value="FAD SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23293">
        <property type="entry name" value="FAD SYNTHETASE-RELATED FMN ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24102">
        <property type="entry name" value="FLAD1_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FAD synthase middle" evidence="5">
        <location>
            <begin position="63"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF24102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8023709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="298" mass="33724" checksum="1BCAD8B6DFC8D17D" modified="2023-09-13" version="1">MKMAHVPESASLTYPINEKTGEKFPYPLVSVRNVYIFPGIPKLLERAFSSLKNTLFGGSEVSFHLQEVYLNQKELDVADIINEFVKKYEGIITVGSYPEWFNSYFSVKVTLETHSKDELNRGYADLERMLPEGSIVEYEKDPIGKAADAVYALAAEEGTFPSKVKHSLAVIEEALDRYTLTDICAGFNGGKDCTALLHLFYAAVKKKYPEYADKLQILYIESIHGTFPDVEKFFKISVERYNLEQLTVKGRIKEGLHELKKTHPQIKAVLMGTRKSDPYSGKTIIVLKINHTIDLLVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YN65</accession>
    <name>A0A9Q0YN65_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Zinc finger BED domain-containing protein 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_33067</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YN65"/>
    <dbReference type="OrthoDB" id="6144063at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45913">
        <property type="entry name" value="EPM2A-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45913:SF19">
        <property type="entry name" value="LOW QUALITY PROTEIN: ZINC FINGER BED DOMAIN-CONTAINING PROTEIN 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8025492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="170" mass="19420" checksum="BD351C78EAC681E9" modified="2023-09-13" version="1">MQVKNSSVFQTCDKIEGFKLKVGLWVNAIDQGNLQMFPTLSEVLISLKCQAPGGIMRKHLQKLSEQLKSYFDYDLRERHKWIRNPFIIGPNDPNKLNIDQVASLMELKCDGSLKTLFREHTLQELWLAIKGEYTVLADKAVAFLLPFCTTWMCESGFQNEGSKQAFNGES</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YP09</accession>
    <name>A0A9Q0YP09_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_33637</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YP09"/>
    <dbReference type="OrthoDB" id="3247158at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027871">
        <property type="entry name" value="DUF4603"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17611">
        <property type="entry name" value="DNA SEGMENT, CHR 5, ERATO DOI 579, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17611:SF3">
        <property type="entry name" value="DNA SEGMENT, CHR 5, ERATO DOI 579, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15376">
        <property type="entry name" value="DUF4603"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="122"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="317"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="474"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="542"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="752"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="949"/>
            <end position="982"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1057"/>
            <end position="1078"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="199"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="222"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="774"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="950"/>
            <end position="964"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8025930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1084" mass="119724" checksum="F4A6C5346749B37C" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YPY5</accession>
    <name>A0A9Q0YPY5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sulfotransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_34131</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8026319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YPY5"/>
    <dbReference type="OrthoDB" id="25921at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48312">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48312:SF1">
        <property type="entry name" value="SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19798">
        <property type="entry name" value="Sulfotransfer_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8026319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="179" mass="21188" checksum="D3FB5BF193A88B56" modified="2023-09-13" version="1">MIRNPLYMYPSFKNTITVDPDFNQVPFESKFTANANIIDDYPFFPVKYYYHFMYRLWKAVGSLQDCNPIVIDADELSRRPDILLRKYLKAVGFPWKEQLLTWDTGKEITRGWKTSLQRMDSTLKTWEVWYQRALESSTFMPNTKPLPSIESLTPDLQACVSTAMPYYEAMFERRIKVHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YQL9</accession>
    <name>A0A9Q0YQL9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_33338</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YQL9"/>
    <dbReference type="OrthoDB" id="5800476at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001123">
        <property type="entry name" value="CK1/CK1/CK1-D protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="190"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="191"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="207"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8025710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="216" mass="25289" checksum="9275DB4D242FE472" modified="2023-09-13" version="1">MIGRIEYVHKKNFIHRDIKPDNFLMGIGRHCNQLFLIDFGLAKKYKDTRTKQHIPYREDKNLTGTARYASINAHLGIEQSRRDDLESLGYVLMYFNRTSLPWQGLKAPTKKAKYEKISERKMSTPVEVLCKGFSAEFAMYLNYARGLRFEEEPDYLYLRQLYRILFRTLNHQYDFTFDWTVQKQKAAAAQTGLSSSASKNPNSHLTKNSESKGKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YQU5</accession>
    <name>A0A9Q0YQU5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_31745</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YQU5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="2">
        <location>
            <begin position="1"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="150"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="281"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="169"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="201"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="219"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8026803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="395" mass="44742" checksum="D8F82C8AB7C2A923" modified="2023-09-13" version="1">MKGKQGNLSVQQTKGRKTKWTKKYALLKADTKELILSDKRIKSDVISDANEKTIRRIVLHTDVTCIPVETEGKRAKRQFLLRIMSDTEDKKSAEEEEEVTLSVTTTEERDEWVKEINDVIDNENDVTEKEEITVEDLDAVKVETDALNHLNKTRPKFQGRRRPQKRPSRMLEEIDENAEKKQKTDNQTDSPVKSACTDATDGASSSGLGLDSEAEVTSKGPSSSCSHDNVNDDENQSRRFKRKGKKSPTNQSPKHTEVRRRPKNDRSTSLLKRLSNRLSAVFGHGSSSTSPIDENEKCEELEASPGEVDRRKSFDSIDISEDMDEDVEGNLSKITDNAEKLLSIAKALQEDLRKGAKNSDLVQMDLKYIRRAMKHLEKSLENISLIEKKQVTLEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YRJ4</accession>
    <name>A0A9Q0YRJ4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Leucine-rich repeat-containing G-protein coupled receptor 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_32348</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YRJ4"/>
    <dbReference type="OrthoDB" id="27267at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050328">
        <property type="entry name" value="Dev_Immune_Receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24373:SF398">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING G-PROTEIN COUPLED RECEPTOR 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24373">
        <property type="entry name" value="SLIT RELATED LEUCINE-RICH REPEAT NEURONAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0675">Receptor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="396"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8027250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="438" mass="49660" checksum="42C76B7C8A8FB147" modified="2023-09-13" version="1">MDFSWPVIHNWDYLVWYITKVFLTLICAINLTDSVPTGHNVSPKAGSPEQTCGNVCRYDEYFRRAQCNERGLTYIPSSTGCEMTTILELQNNNIQNIPPERIEEYYHVKTMGLSWNQISVLYPGTFSRLSRLRNMICSHNNLEIVPNGVFNGTEESLSRIYLNNNSIIIIGNRAFQGLAQVTFINLNDNLIKALPVRVFRDVINIKDLFLDNNELKYLNKDQFKGLKEMSTLSLQGNQIKTLPSGLFSGLESLNEVSISNNQLITILAPQILGLRLGVNVLNLTNNHMSHTAVIFPFLINATWTLYLGGNPFLCDCDFQMLQERLNTTSTQQTIDTSSENPLSCEFQDGTKQNITDSLSNLCHDTTTKEREIDNWFNRSGSTNQHGQLTSSETNPGWLPIFIFTELTLFFILWLGNTFYKPCKKAGNVIKAKIAKVQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YSF6</accession>
    <name>A0A9Q0YSF6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Metabotropic glutamate receptor 8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_32106</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YSF6"/>
    <dbReference type="OrthoDB" id="425344at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13953">
        <property type="entry name" value="7tm_classC_mGluR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.30">
        <property type="entry name" value="GPCR, family 3, nine cysteines domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011500">
        <property type="entry name" value="GPCR_3_9-Cys_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038550">
        <property type="entry name" value="GPCR_3_9-Cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050726">
        <property type="entry name" value="mGluR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24060">
        <property type="entry name" value="METABOTROPIC GLUTAMATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07562">
        <property type="entry name" value="NCD3G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="287"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="326"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="358"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="399"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="7">
        <location>
            <begin position="288"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8027071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="497" mass="55533" checksum="440207017DD60179" modified="2023-09-13" version="1">MPLPLSTALTTIVSSFTGCFTVLPLSGTLTSFDDYFRSLSESNSHNPWLQDFSQAYGNCNGDVRTKSVSVDCQPFIDLDASDSPTHETLVIDSVNALAYALDNMHKDHCPDSPMDLCDDMQIVNGEEFKQYLLNVKVNSVANGEVSFLPNGDSQGKYSVRNLQIVDGTYQFVDVGKWVFSDTGKCLAIDENIPWYVGDFRERDTETGAPKSICSDPCDIGERRTPVPENSCCWKCTRCDVDEIVTENGTRCESCVNVAEDKYDWPDKSSNYTICIPIDAKDSLRENILGIVVVVFSSIGLVLTAIILSVYIKNREEPLIKASSRELCYVIYTGICLAYIVALANGLPPRSVTCVFRRIGPVLATSLIYIPLCTKTIRLYRIFQSGRKSARRPKFISPGFQMTLTFSITAIPVSLLIFVPHFHLSLFKPELSANVEYNMDRGDTPGRGGFHAFREPPSSGTDMRRQHPIIRWDHALGRYSGLCMLRLRIHDSQITRQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1B9S7</accession>
    <name>A0A9Q1B9S7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">V(D)J recombination activating protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_43231</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000241">
        <property type="protein sequence ID" value="KAJ8018665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1B9S7"/>
    <dbReference type="OrthoDB" id="10069097at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009689">
        <property type="entry name" value="DUF1280"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31424">
        <property type="entry name" value="PROTEIN CBG23806"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31424:SF3">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06918">
        <property type="entry name" value="DUF1280"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="155"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8018665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="705" mass="79475" checksum="C80A026F92DF707D" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BD77</accession>
    <name>A0A9Q1BD77_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_40959</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8021168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BD77"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8021168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="187" mass="20310" checksum="5F526FE2942BBF68" modified="2023-09-13" version="1">MAIVARSMSCWFCDHMKFSSITLKTSCSSYFYRCQLGFTGTQCESSTFTAPPPITVASGRGLSQAAIIGIIAGALALLLLLLLLACCCCFMLFAGQPKPGPVVGPISPPVPYEEPVAIAPPRIPRAIAYSAPPPYIQEEPFVEMLVPEPVTPSSLVSYHGPAYRSGPIIEEIDSYHPYKRPYLDPYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BDN1</accession>
    <name>A0A9Q1BDN1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="22">Activated CDC42 kinase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">HOLleu_38557</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="22">Nanhai2018</strain>
            <tissue evidence="22">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Clathrin-coated vesicle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the protein kinase superfamily. Tyr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BDN1"/>
    <dbReference type="OrthoDB" id="635774at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05040">
        <property type="entry name" value="PTKc_Ack_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14328">
        <property type="entry name" value="UBA_TNK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000080">
        <property type="entry name" value="Putative activated CDC42 kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000107">
        <property type="entry name" value="Putative activated CDC42 kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.680.10">
        <property type="entry name" value="Cdc42-like binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015116">
        <property type="entry name" value="Cdc42-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037085">
        <property type="entry name" value="Cdc42-bd-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050198">
        <property type="entry name" value="Non-receptor_tyrosine_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24418">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09027">
        <property type="entry name" value="GTPase_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="9 22" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3 16" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="12" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="Protein kinase" evidence="20">
        <location>
            <begin position="64"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="19">
        <location>
            <begin position="336"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="21">
        <location>
            <begin position="1195"/>
            <end position="1240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="463"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="695"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="865"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="899"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="938"/>
            <end position="972"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1064"/>
            <end position="1094"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1119"/>
            <end position="1166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="470"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="938"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="1064"/>
            <end position="1093"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00060742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KAJ8021379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1247" mass="138005" checksum="3522436F81445832" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BDS0</accession>
    <name>A0A9Q1BDS0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Splicing factor 3B subunit 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_38952</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SF3B4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BDS0"/>
    <dbReference type="OrthoDB" id="10259687at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048026">
        <property type="term" value="P:positive regulation of mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12334">
        <property type="entry name" value="RRM1_SF3B4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12335">
        <property type="entry name" value="RRM2_SF3B4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000059">
        <property type="entry name" value="splicing factor 3B subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000141">
        <property type="entry name" value="Splicing factor 3b subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034158">
        <property type="entry name" value="SF3B4_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034159">
        <property type="entry name" value="SF3B4_RRM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052084">
        <property type="entry name" value="SF3B4_spliceosome_assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48030">
        <property type="entry name" value="SPLICING FACTOR 3B SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48030:SF3">
        <property type="entry name" value="SPLICING FACTOR 3B SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="13"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="100"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="222"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="222"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="252"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="356"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="380"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="402"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00070533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8021678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="434" mass="45753" checksum="88B419668A9E060B" modified="2023-09-13" version="1">MAAGPPQERNQDATVYVGGLDEKVTEAILWELFLQAGPVVNTHMPKDRVTQMHQGYGFIEFMGEEDADYAIKVMNMIKIYGKPIRVNKASAHQKNLDVGANIFIGNLDPEIDEKLLFDTFSAFGVILQTPKIMRDPDTGNSKGFAFINFASFEAADAAIEAMNGQYLCNRAITISFAFKKDSKGERHGSAAERLLAKQNPLAQADRPHQLFADAPPTSQSIAANQAAVAAAVAQPPPPPPTAGLTSSTGLSVPPPSTTPLPPFPPTSMPPPPMGMPPGMPPPPVPPSGMPPMQMPPAMPSAGGPPPMPPRPPMPPPPGAPFPPPPSGQMPPGFPPGMGPPPGMPPPPPMSQQGQRGPPPFPPPFGGPRDQQGSGDAGQGGPPPFMPPRPPPIGQGNYDRPPRPPPFPRGPPRGPPPRGPPPLMPPRMRPPPPRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BGE8</accession>
    <name>A0A9Q1BGE8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_36943</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8024259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BGE8"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="101"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8024259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="125" mass="13500" checksum="212286480D20AB51" modified="2023-09-13" version="1">MECCDRDYCNGGCTKNEGKSQEELSKGNEEVNEGSNEESNGRRNGDDSGRSNEEHDERNNGKSNGRRNENGKGNKEGNGKASAGSRESDGPVNNNNRDQQIVGGGALHVPTVWAMLFGFLLLVVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BHZ2</accession>
    <name>A0A9Q1BHZ2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_33249</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BHZ2"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8025637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="274" mass="31378" checksum="C73E796B8CADC3F7" modified="2023-09-13" version="1">MEDNQILPEVPDDLYNAGWRVHYNMEDDKQYFVNEQTKMRSDTLPTVVKSPSTNLTLFENANIDLTEGFVPSFYTEAKESERNLATTSGQTPNEEKPIEKGEAPGKLYQKLMEESTTRFPLGVCSGEIKISDTAREMVAYIFASMIDRVAYGYLKQNCEGCDMGWPSQMDHRCLNFGSNVSVADMYFNDMQHLVKDEWVVNVVRDYLLKSVESHTVRAVMQQLRTSWSQSPELVVEAMETSRVNAIIKGHLEDEFIPRWMGTVDYSSVELNYTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BI85</accession>
    <name>A0A9Q1BI85_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Neuronal acetylcholine receptor subunit alpha-6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_31461</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the ligand-gated ion channel (TC 1.A.9) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BI85"/>
    <dbReference type="OrthoDB" id="6097796at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005230">
        <property type="term" value="F:extracellular ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18989">
        <property type="entry name" value="LGIC_ECD_cation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19051">
        <property type="entry name" value="LGIC_TM_cation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="155"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="185"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="222"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="328"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="6">
        <location>
            <begin position="2"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="7">
        <location>
            <begin position="160"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8026584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="359" mass="40377" checksum="3C94DEED9202F071" modified="2023-09-13" version="1">MRLSWDPSEFDGVGDIVLDVDEIWTPKIYLSNSVSKDSLHVISRERGSVILSSQGHVQLGTPIVVSTHCPMQVRYFPFDVQVCQFNFMPVNQLFDQMALSAQQSQNPSALVSLQWNLLNCTVTSYVYHFHDPNTNKSASFTGVLICLYLQRNPNYYISNIIVPSTFMCCLAFATFLATPESGERISLGVSMVLGLTVFQLLIADTLPISSKQSPILTNYLAVNFILACLVVPLSLANINIACMDKKLKILKYGWVEKVFVEWLPCCLCVSTIDERETESNQADPLKVTNDQGQLETKDAINDKRLKEYSVDQISPTTWEHYSTSTTKVTNIVFTFFLLCLFLGYSLDKSNHISAKEPKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BJ30</accession>
    <name>A0A9Q1BJ30_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_32701</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BJ30"/>
    <dbReference type="OrthoDB" id="425619at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8027534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="102" mass="12048" checksum="76620B646467AE3B" modified="2023-09-13" version="1">MPFNLDKFVTSPSVEELDSLKKSEIVKVAKHYGIEFQPLMRKDEIKRYVLEYLVDEGVLPSTVLETAITVPTDNTFELKRLELEMNKEIRLKEMEREMQKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BJM8</accession>
    <name>A0A9Q1BJM8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_29937</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8027863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BJM8"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8027863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="51" mass="5902" checksum="6D66848EE9FEA098" modified="2023-09-13" version="1">MTLTIVSINGVVLSVVVSRNIIFLALKCYRYISLILFLCLNYLNNVNFSFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BKE8</accession>
    <name>A0A9Q1BKE8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_30260</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BKE8"/>
    <dbReference type="OrthoDB" id="416454at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8028114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="52" mass="5986" checksum="B9D9F00AA955A27F" modified="2023-09-13" version="1">MEIINWLLSLGEFPEQLKHALVQPLLKKHSLDPEILRNCRPVSNLSFISTTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BL22</accession>
    <name>A0A9Q1BL22_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_30692</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BL22"/>
    <dbReference type="OrthoDB" id="6502630at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8028459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="135" mass="15299" checksum="E3ED82BCD03447DC" modified="2023-09-13" version="1">MRYGKRKNPIIFSGGQRSSGVNGGQTLKTLLTRYLKKGSSDSSQTWYLPYGKRKNRIIFEGGQRSSGVTRGQTLKTLLTQYLEMRYGERKNPIIFGGGQRSSGVTRGQTLKTLLTRYLEAESLDKFHTVYVDAFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BLP4</accession>
    <name>A0A9Q1BLP4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DmX-like protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_28242</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8028972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="342131at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043291">
        <property type="term" value="C:RAVE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052208">
        <property type="entry name" value="DmX-like/RAVE_component"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022033">
        <property type="entry name" value="Rav1p_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13950:SF9">
        <property type="entry name" value="RABCONNECTIN-3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13950">
        <property type="entry name" value="RABCONNECTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12234">
        <property type="entry name" value="Rav1p_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RAVE complex protein Rav1 C-terminal" evidence="2">
        <location>
            <begin position="1170"/>
            <end position="1405"/>
        </location>
    </feature>
    <feature type="domain" description="RAVE complex protein Rav1 C-terminal" evidence="2">
        <location>
            <begin position="1601"/>
            <end position="1911"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="274"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="883"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2007"/>
            <end position="2084"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="275"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="301"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="887"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="2045"/>
            <end position="2056"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="2057"/>
            <end position="2074"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="2075"/>
            <end position="2084"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8028972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="2403" mass="266336" checksum="67ECA634DE4AA818" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BMH4</accession>
    <name>A0A9Q1BMH4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Neurotrypsin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_28629</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BMH4"/>
    <dbReference type="OrthoDB" id="536948at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19331:SF465">
        <property type="entry name" value="EGG PEPTIDE SPERACT RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19331">
        <property type="entry name" value="SCAVENGER RECEPTOR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00530">
        <property type="entry name" value="SRCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00258">
        <property type="entry name" value="SPERACTRCPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 7" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040514569" evidence="7">
        <location>
            <begin position="26"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="149"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="8">
        <location>
            <begin position="31"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="97"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8029274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="240" mass="26174" checksum="586DEA1F3A1E22F2" modified="2023-09-13" version="1">MDVLGIAFVRTLVCLLFIYVGVCESQEDRSVRIVSGPREGRVEIYFDGQWSTVSQDGWTETDAGVVCNQLGFKGPAIKTTEGPATLAFDVPKLSVNCPKGAKSLSECDIEKTAGSALDASASYLDGQTYWDHSQSAGVKCETESNVVQIVINVVGIVVVIAIICVFATLFYRFKKNQVPKESKPEEAKQETVAMYATQLPKPFADNPDDRTAQYCSINPVAVELENSYLELNQGDSPQQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BQ10</accession>
    <name>A0A9Q1BQ10_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_02799</name>
        <name evidence="1" type="ORF">HOLleu_27069</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BQ10"/>
    <dbReference type="OrthoDB" id="5986074at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8030608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8049872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="293" mass="32464" checksum="03FDEFD885E1A1B4" modified="2023-09-13" version="1">MLPLVKLMTVMEQGQGLFDRFILTAPMCLRPTPEETSAAIQTLKGMDISDVGIVYETIFDLHLQEQMYTLDNEAQKLYTDLNKAFIDDINEALLNGTVPPRSKKVDIVGRIALSVHVFDAVARALLSGENCDIAPSINHHTLKSALALVEFSDCQKTILIDALDEILLPTRDDVKAQPSLEEVQEAILTSPGPVTTARSFKQGAPRHYRSTTSAEFSKAIEGMLDYGTVLGIRVPRRASKVQVFCKKSPDVLQERWPSDAPCSFQSYSGAFKKNLPTAISDYMKIELNKKGFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BQ26</accession>
    <name>A0A9Q1BQ26_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Integrase core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_27528</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BQ26"/>
    <dbReference type="OrthoDB" id="5948209at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058913">
        <property type="entry name" value="Integrase_dom_put"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46791:SF13">
        <property type="entry name" value="CLR5 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46791">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24764">
        <property type="entry name" value="rva_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase core" evidence="1">
        <location>
            <begin position="122"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF24764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8030963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="392" mass="46381" checksum="3144AC1AFAF07D8D" modified="2023-09-13" version="1">MDDYRMARIRLFFRLGLTNDEILIALSELDNIELSKRHLKRLMNSEGLFRRKHHSNYLADVALYVISELERFGQLHGYRWFHHKLRNNGYNIDRETVRRLLLLFDPDGVELRRRRRLRRRNYNGIGPNFVWHIDSYDKLKHFGICINGCIDGFSRYILWLEAYKSSSDPRLIAGYYMNTVDRTGGCPTVVRADLGTENSSVRDMQIFLRRNGRDRHAAERSFLYGRSTSNQRIEFWWSILRKQCADFWINLFGVLRDIEAIFDGGDLDKALIQFCFMSIIQEELDNTVLQWSTHRISPSRLHPGPFGRPVMMYISPRSYGTRDYLHNVERDELEVCLEECRFKDGFPCCQEVFDVCCVIMNEQNLLPPSSPSEAVELYQILRQALRNMLRIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BQB3</accession>
    <name>A0A9Q1BQB3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_27137</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BQB3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8030666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="62" mass="7384" checksum="DF3FA874AC022EFE" modified="2023-09-13" version="1">MDRKRLDSKSTKDSWDVPPLQQRLTRFPTGMMNDMEKKRKEEVDKHKGEPTLSVERLEPDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BRN7</accession>
    <name>A0A9Q1BRN7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">U2 snRNP-associated SURP motif-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_28063</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8031356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BRN7"/>
    <dbReference type="OrthoDB" id="377209at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21370">
        <property type="entry name" value="cwf21_SR140"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.790">
        <property type="entry name" value="Surp module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006569">
        <property type="entry name" value="CID_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013170">
        <property type="entry name" value="mRNA_splic_Cwf21_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051485">
        <property type="entry name" value="SR-CTD_assoc_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047488">
        <property type="entry name" value="SR140_cwf21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000061">
        <property type="entry name" value="Surp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035967">
        <property type="entry name" value="SWAP/Surp_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23140">
        <property type="entry name" value="RNA PROCESSING PROTEIN LD23810P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23140:SF0">
        <property type="entry name" value="U2 SNRNP-ASSOCIATED SURP MOTIF-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04818">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01805">
        <property type="entry name" value="Surp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01115">
        <property type="entry name" value="cwf21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00582">
        <property type="entry name" value="RPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00648">
        <property type="entry name" value="SWAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109905">
        <property type="entry name" value="Surp module (SWAP domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51391">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50128">
        <property type="entry name" value="SURP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="SURP motif" evidence="3">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="CID" evidence="4">
        <location>
            <begin position="114"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="81"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="369"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="103"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="387"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="422"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="448"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="471"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="532"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="585"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="620"/>
            <end position="639"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8031356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="639" mass="73694" checksum="401ED826F87257EF" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BSX0</accession>
    <name>A0A9Q1BSX0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 13">Coatomer subunit beta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11 13">Beta-coat protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HOLleu_25351</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Nanhai2018</strain>
            <tissue evidence="17">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. Required for limiting lipid storage in lipid droplets.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 13">Golgi apparatus membrane</location>
            <topology evidence="1 13">Peripheral membrane protein</topology>
            <orientation evidence="1 13">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Cytoplasmic vesicle</location>
            <location evidence="13">COPI-coated vesicle membrane</location>
            <topology evidence="13">Peripheral membrane protein</topology>
            <orientation evidence="13">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BSX0"/>
    <dbReference type="OrthoDB" id="10261439at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030126">
        <property type="term" value="C:COPI vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000311">
        <property type="entry name" value="Coatomer subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011710">
        <property type="entry name" value="Coatomer_bsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016460">
        <property type="entry name" value="COPB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029446">
        <property type="entry name" value="COPB1_appendage_platform_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10635">
        <property type="entry name" value="COATOMER SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10635:SF0">
        <property type="entry name" value="COATOMER SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07718">
        <property type="entry name" value="Coatamer_beta_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14806">
        <property type="entry name" value="Coatomer_b_Cpla"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005727">
        <property type="entry name" value="Coatomer_beta_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 13" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6 13" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8 13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 13" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 13" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Clathrin/coatomer adaptor adaptin-like N-terminal" evidence="14">
        <location>
            <begin position="23"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="domain" description="Coatomer beta subunit C-terminal" evidence="15">
        <location>
            <begin position="668"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="domain" description="Coatomer beta subunit appendage platform" evidence="16">
        <location>
            <begin position="815"/>
            <end position="946"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00058599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR005727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF07718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF14806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ8031969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="957" mass="107192" checksum="C520A42996CB8EA7" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BV67</accession>
    <name>A0A9Q1BV67_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Tenascin-R</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_23325</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BV67"/>
    <dbReference type="OrthoDB" id="6145874at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050373">
        <property type="entry name" value="Fibrinogen_C-term_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143">
        <property type="entry name" value="FIBRINOGEN/TENASCIN/ANGIOPOEITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8033171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="76" mass="8739" checksum="08A7B5A01232FEA5" modified="2023-09-13" version="1">MAYHNTFQFSTRDRDNDNHPTVDCAGNAQGAWWFKQCWKIHLNGPYGSPEPPGVCYIIDTVRHCNVPSTEMSIRRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BYD6</accession>
    <name>A0A9Q1BYD6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_22297</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BYD6"/>
    <dbReference type="OrthoDB" id="2286242at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8035162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="117" mass="13323" checksum="CA2DA9D5C23E84B7" modified="2023-09-13" version="1">MFRGYGVMTDVKCKIQLKEDASPVCIMTPRKVSQPLLPKIKVSLDKMEKLNVISLVKEPTEWCPGIVCVRKPDDTVRICVNLTELNKSVKREVYPMENVDANLAQLGTQYHVYKTIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BZZ0</accession>
    <name>A0A9Q1BZZ0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Protein ALP1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_19623</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HARBI1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8035827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BZZ0"/>
    <dbReference type="OrthoDB" id="1681765at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045249">
        <property type="entry name" value="HARBI1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027806">
        <property type="entry name" value="HARBI1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930:SF269">
        <property type="entry name" value="NUCLEASE HARBI1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13359">
        <property type="entry name" value="DDE_Tnp_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE Tnp4" evidence="8">
        <location>
            <begin position="100"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8035827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="339" mass="38367" checksum="639EE23F9BD1F6E8" modified="2023-09-13" version="1">MVRPIIQKKDTIMREAISANERLSITLRFLATGNSFEDLKFITRTSPQAIGKIVFETCEVITQCLQRQQAIKIPRTPEEWREISNAFATTWNFPHCVGAIDGKHVAIRKPPESGSFYFNYKKFFSIVLMAVVGADYEFIMVDAGVNGRVSDGGVIAVTEFGRLLDDGSLGLPEPAPLHQNDVIAMPYVFVGDDAFAMSENLLKPYGGDRLESDQRIYNYRLSRARRVTENAFGILTARFGVFQKAMQLSPEKATLITMTCCYLHNFLRKKSRCYIGPGAVDWEDANHEMHAGEWRASQRQLEGLRATMNRNAGNTARLVRDAFRHYFCTQGQVPWQDSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C1P7</accession>
    <name>A0A9Q1C1P7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Paired amphipathic helix protein Sin3a</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Histone deacetylase complex subunit Sin3a</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Transcriptional corepressor Sin3a</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">HOLleu_20652</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">Nanhai2018</strain>
            <tissue evidence="21">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Acts as a transcriptional repressor. Corepressor for REST. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Also interacts with MXD1-MAX heterodimers to repress transcription by tethering SIN3A to DNA. Acts cooperatively with OGT to repress transcription in parallel with histone deacetylation. Involved in the control of the circadian rhythms. Required for the transcriptional repression of circadian target genes, such as PER1, mediated by the large PER complex through histone deacetylation. Cooperates with FOXK1 to regulate cell cycle progression probably by repressing cell cycle inhibitor genes expression. Required for cortical neuron differentiation and callosal axon elongation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Interacts with ARID4B, BRMS1L, HCFC1, HDAC1, HDAC2, MXI1, SAP30L, SAP130, SFPQ and TOPORS. Interacts with OGT (via TPRs 1-6); the interaction mediates transcriptional repression in parallel with histone deacetylase. Interacts with BAZ2A, MXD1, MXD3, MXD4, MBD2, DACH1, NCOR1, NR4A2, REST, RLIM, SAP30, SETDB1, SMYD2, and SUDS3. Interacts with PHF12 in a complex composed of HDAC1, PHF12 and SAP30. Interacts with TET1; the interaction recruits SIN3A to gene promoters. The large PER complex involved in the histone deacetylation is composed of at least HDAC1, PER2, SFPQ and SIN3A. Interacts with KLF11. Interacts with PPHLN1. Found in a complex with YY1, GON4L and HDAC1. Interacts (via PAH2) with FOXK1. Interacts with FOXK2. Found in a complex composed of at least SINHCAF, SIN3A, HDAC1, SAP30, RBBP4, OGT and TET1. Interacts with SINHCAF. Interacts with SPHK2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10265969at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070822">
        <property type="term" value="C:Sin3-type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061629">
        <property type="term" value="F:RNA polymerase II-specific DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048511">
        <property type="term" value="P:rhythmic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1160.11:FF:000001">
        <property type="entry name" value="Paired amphipathic helix protein Sin3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1160.11:FF:000002">
        <property type="entry name" value="Paired amphipathic helix protein SIN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1160.11:FF:000004">
        <property type="entry name" value="Paired amphipathic helix protein Sin3a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1160.11">
        <property type="entry name" value="Paired amphipathic helix"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013194">
        <property type="entry name" value="HDAC_interact_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003822">
        <property type="entry name" value="PAH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036600">
        <property type="entry name" value="PAH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039774">
        <property type="entry name" value="Sin3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031693">
        <property type="entry name" value="Sin3_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12346:SF0">
        <property type="entry name" value="SIN3A, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12346">
        <property type="entry name" value="SIN3B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02671">
        <property type="entry name" value="PAH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08295">
        <property type="entry name" value="Sin3_corepress"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16879">
        <property type="entry name" value="Sin3a_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00761">
        <property type="entry name" value="HDAC_interact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47762">
        <property type="entry name" value="PAH2 domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51477">
        <property type="entry name" value="PAH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="10" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="12 18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Histone deacetylase interacting" evidence="20">
        <location>
            <begin position="582"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="51"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="285"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="442"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="860"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1162"/>
            <end position="1243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="17"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="79"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="120"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="19">
        <location>
            <begin position="306"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="320"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="454"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="860"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="875"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="1175"/>
            <end position="1237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00056268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00061761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00068512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00075105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00081271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM00761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAJ8036623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1361" mass="153907" checksum="63AE19BEAFE4E662" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C2C9</accession>
    <name>A0A9Q1C2C9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TGF-beta-activated kinase 1 and MAP3K7-binding protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_18811</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C2C9"/>
    <dbReference type="OrthoDB" id="10049211at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832:SF533">
        <property type="entry name" value="TGF-BETA-ACTIVATED KINASE 1 AND MAP3K7-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="2">
        <location>
            <begin position="23"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="394"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="394"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="411"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8037878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="481" mass="53115" checksum="AB1919BF28A2A306" modified="2023-09-13" version="1">MADDEDREEQVWTDALPVCQHSGIGSLQNQGYKADGTRVTYRDSEDLFFHFRTDDANTYLYAIFDGYDGKEVAAFAHQRLPAELLLGQIEGVTDDDQVKQILSQAILIVERSYFETIDGLLAQKTMLSMNLPEGFNMRQTFQHQEEIAQLKEIEEKLKGGTALIVALLHRKKLYVANVGDSRALLCTKEENGALTVTKVSVDHNIANDDEVLRLEQLGLGTNVFQGEPLIGDSPATRTIGNYRVKGGYRDNPDLRCASKEPVIADPDIVGGIKMDGKVGFLVMFTHGFWQALEAATGTTNIDTDIVNMVAKEFSQQGTLSSVAQAVVDRVVRMHHDAFMSGGQQKELKRREDITVIVRNFDFPLGHTRHIYIPPRHVNVAQEYPVITPIYSPSSGSVNGGSSPSTPLPFDQTPTPNTTPKISRLESSGGVSTNSSEDSDQRPLSGSLGEAEEEEKMIDAYVNFNDLNIALLNTDTAEVYIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C2D7</accession>
    <name>A0A9Q1C2D7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Small G protein signaling modulator 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">RUN and TBC1 domain-containing protein 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_17857</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small G protein signaling modulator family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C2D7"/>
    <dbReference type="OrthoDB" id="44736at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17688">
        <property type="entry name" value="RUN_SGSM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11813">
        <property type="entry name" value="SH3_SGSM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000012">
        <property type="entry name" value="Small G protein signaling modulator 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000013">
        <property type="entry name" value="Small G protein signaling modulator 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.900:FF:000016">
        <property type="entry name" value="Small G protein signaling modulator 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000115">
        <property type="entry name" value="Small G protein signaling modulator 3 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004012">
        <property type="entry name" value="Run_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037213">
        <property type="entry name" value="Run_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035833">
        <property type="entry name" value="SGSM3_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF13">
        <property type="entry name" value="RUN AND TBC1 DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02759">
        <property type="entry name" value="RUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00593">
        <property type="entry name" value="RUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140741">
        <property type="entry name" value="RUN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50826">
        <property type="entry name" value="RUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="8">
        <location>
            <begin position="153"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="7">
        <location>
            <begin position="540"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="domain" description="RUN" evidence="9">
        <location>
            <begin position="615"/>
            <end position="778"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00030864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00073333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8037117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="807" mass="92257" checksum="9DA4E263DA136104" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C3X9</accession>
    <name>A0A9Q1C3X9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">28 kDa heat- and acid-stable phosphoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_19179</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8038177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C3X9"/>
    <dbReference type="OrthoDB" id="21120at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019380">
        <property type="entry name" value="Casein_kinase_sb_PP28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039876">
        <property type="entry name" value="HAP28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22055">
        <property type="entry name" value="28 KDA HEAT- AND ACID-STABLE PHOSPHOPROTEIN PDGF-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10252">
        <property type="entry name" value="PP28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Casein kinase substrate phosphoprotein PP28" evidence="2">
        <location>
            <begin position="91"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="157"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8038177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="181" mass="21101" checksum="DF7D6CAB34DAB072" modified="2023-09-13" version="1">MSRRGGKKSHKGGRKQYTNPDEIDAQMKEEELRRQRREEQGDEGEEDISTKMRQPPRDFPSSSSEESESDSDDEDYKDKKKGVSHLIEIENPNRVKNKMKKAKDVDLNQPAAQQLSRREREEIEKQQERSKYRALHAQGKTQEARADLARLAIIKKQRDDAAKKREAEQKAKEAAKENKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C4M2</accession>
    <name>A0A9Q1C4M2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Arylsulfatase G</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_19039</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sulfatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8038064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C4M2"/>
    <dbReference type="OrthoDB" id="103349at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004065">
        <property type="term" value="F:arylsulfatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050738">
        <property type="entry name" value="Sulfatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024607">
        <property type="entry name" value="Sulfatase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000917">
        <property type="entry name" value="Sulfatase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42693">
        <property type="entry name" value="ARYLSULFATASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42693:SF42">
        <property type="entry name" value="ARYLSULFATASE G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00884">
        <property type="entry name" value="Sulfatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14707">
        <property type="entry name" value="Sulfatase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00523">
        <property type="entry name" value="SULFATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Sulfatase N-terminal" evidence="7">
        <location>
            <begin position="19"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8038064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="517" mass="56586" checksum="C11BB90A5F216AFD" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-10-08" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C6C4</accession>
    <name>A0A9Q1C6C4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Helicase with zinc finger domain 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_16585</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA2/NAM7 helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2285229at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:RNA nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001313">
        <property type="entry name" value="Helicase with zinc finger domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050534">
        <property type="entry name" value="Coronavir_polyprotein_1ab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001900">
        <property type="entry name" value="RNase_II/R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43788">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43788:SF16">
        <property type="entry name" value="HELICASE WITH ZINC FINGER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00773">
        <property type="entry name" value="RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00955">
        <property type="entry name" value="RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 8" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="430"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1584"/>
            <end position="1623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="1609"/>
            <end position="1620"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8039005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="3255" mass="372780" checksum="99A49B79DDC9F810" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C6Q0</accession>
    <name>A0A9Q1C6Q0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_16822</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C6Q0"/>
    <dbReference type="OrthoDB" id="8056871at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8039185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="126" mass="14554" checksum="E28DE3AEE8C3A8C3" modified="2023-09-13" version="1">MDDKLKHPPSKSEDTVPKSGTNDPTRTNLIEHDVDVGDATPIKQHPYRVNPRKRQHLRDEVRYMLDHDIIEPSNSEWSSPCILVPKPDVIRSWNQFLRYSTLQVYYCTIVDVHALPALGPDQNNIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C727</accession>
    <name>A0A9Q1C727_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Copper transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_16979</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the copper transporter (Ctr) (TC 1.A.56) family. SLC31A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C727"/>
    <dbReference type="OrthoDB" id="161814at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005375">
        <property type="term" value="F:copper ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007274">
        <property type="entry name" value="Cop_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483:SF115">
        <property type="entry name" value="COPPER TRANSPORT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 31 COPPER TRANSPORTERS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04145">
        <property type="entry name" value="Ctr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0186">Copper</keyword>
    <keyword evidence="4" id="KW-0187">Copper transport</keyword>
    <keyword evidence="4" id="KW-0406">Ion transport</keyword>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="27"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="91"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="116"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8039309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="148" mass="16828" checksum="58256E3D3F9D8309" modified="2023-09-13" version="1">MSYVYFHLNYTATILVEQWSTTGTWDLFGSCLAVFVLAILYEGLKVFREVMLRSALINVRYNTSGISKNKDTVLVETKGLARTHFCSCSHFLQTFLHIIQTAIGYCLMLVVMTFNLWLTLSIVFGAGAGYFVFGWRKSIVVDINESLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C782</accession>
    <name>A0A9Q1C782_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Valine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Valyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HOLleu_17106</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Nanhai2018</strain>
            <tissue evidence="15">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>tRNA(Val) + L-valine + ATP = L-valyl-tRNA(Val) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:10704"/>
            <dbReference type="Rhea" id="RHEA-COMP:9672"/>
            <dbReference type="Rhea" id="RHEA-COMP:9708"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57762"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78537"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.9"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.9" evidence="2"/>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C782"/>
    <dbReference type="OrthoDB" id="629407at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004832">
        <property type="term" value="F:valine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006438">
        <property type="term" value="P:valyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07962">
        <property type="entry name" value="Anticodon_Ia_Val"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00817">
        <property type="entry name" value="ValRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000119">
        <property type="entry name" value="Putative valine--tRNA ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.380:FF:000002">
        <property type="entry name" value="Valine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.730.10:FF:000015">
        <property type="entry name" value="Valine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000020">
        <property type="entry name" value="Valine--tRNA ligase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.740.10:FF:000005">
        <property type="entry name" value="Valine--tRNA ligase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.380">
        <property type="entry name" value="Valyl-tRNA synthetase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02004">
        <property type="entry name" value="Val_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033705">
        <property type="entry name" value="Anticodon_Ia_Val"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010978">
        <property type="entry name" value="tRNA-bd_arm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037118">
        <property type="entry name" value="Val-tRNA_synth_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002303">
        <property type="entry name" value="Valyl-tRNA_ligase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004349">
        <property type="entry name" value="PRK05729.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00422">
        <property type="entry name" value="valS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11946:SF109">
        <property type="entry name" value="VALINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11946">
        <property type="entry name" value="VALYL-TRNA SYNTHETASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00986">
        <property type="entry name" value="TRNASYNTHVAL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46589">
        <property type="entry name" value="tRNA-binding arm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="ValRS/IleRS/LeuRS editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 11 15" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="13">
        <location>
            <begin position="114"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="domain" description="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding" evidence="14">
        <location>
            <begin position="790"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1010"/>
            <end position="1047"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="40"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="87"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1010"/>
            <end position="1028"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ8039399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1072" mass="122102" checksum="02013AF02D075322" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C794</accession>
    <name>A0A9Q1C794_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Protein NYNRIN</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_13825</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8039730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C794"/>
    <dbReference type="OrthoDB" id="418169at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon gypsy-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF1">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="268"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase catalytic" evidence="4">
        <location>
            <begin position="628"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="19"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="233"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="283"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="437"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="22"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="245"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="451"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="470"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8039730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1132" mass="126687" checksum="B0BD6C524C28729C" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C7K0</accession>
    <name>A0A9Q1C7K0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_13692</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8039635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C7K0"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8039635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="67" mass="7397" checksum="987D288FF73D56FA" modified="2023-09-13" version="1">MSKSTEPPVLSGVAVTVAVAVYECELFCYFVLHCDCASGGKVRFTGIFGSVVYGCTKKWAYFHFFLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C7K4</accession>
    <name>A0A9Q1C7K4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nucleoredoxin-like protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_14375</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C7K4"/>
    <dbReference type="OrthoDB" id="409136at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045494">
        <property type="term" value="P:photoreceptor cell maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007600">
        <property type="term" value="P:sensory perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029519">
        <property type="entry name" value="RdCVF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012336">
        <property type="entry name" value="Thioredoxin-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46762">
        <property type="entry name" value="NUCLEOREDOXIN-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46762:SF1">
        <property type="entry name" value="NUCLEOREDOXIN-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13905">
        <property type="entry name" value="Thioredoxin_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="1">
        <location>
            <begin position="1"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8040157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="153" mass="17561" checksum="3267E73D67350CD7" modified="2023-09-13" version="1">MNYFEGKTLFRMNDDEESDLVNHNEVLGGKILALYFSASWSPPCCEFSPPLIAVYQELIERKMPFEVVYLSCDKNKGEMISYLQELHVPWLYLPFGDKLISDLKEKFNIIAVPKLIVLSPSGEVITDMGRREVQDRGVVCFNSWKQASGMTDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C9I4</accession>
    <name>A0A9Q1C9I4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Growth/differentiation factor 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_15256</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the TGF-beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C9I4"/>
    <dbReference type="OrthoDB" id="5948587at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001839">
        <property type="entry name" value="TGF-b_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015615">
        <property type="entry name" value="TGF-beta-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017948">
        <property type="entry name" value="TGFb_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848">
        <property type="entry name" value="TGF-BETA FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00019">
        <property type="entry name" value="TGF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00204">
        <property type="entry name" value="TGFB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00250">
        <property type="entry name" value="TGF_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51362">
        <property type="entry name" value="TGF_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 6" id="KW-0339">Growth factor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040464392" evidence="7">
        <location>
            <begin position="26"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="domain" description="TGF-beta family profile" evidence="8">
        <location>
            <begin position="265"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8040840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="383" mass="44143" checksum="C0F326090CA38086" modified="2023-09-13" version="1">MNSLRKNLAAFNAFIILVSPFFIRCHPCGVCSQENLGQALSSSLSREHRNQAIKNFFMKKMNRSNTESSSLSISSEILENAKIQYERLMKQKNPVVATIIHEIPGIESPPYHIPADRWRFRLQNHTYRYFFHPRRGGSFYPKLEKLVTAKLILQVPAVLRNLTDLDFPSKLRIDVYQIMEFTDSDQRPRRRLVDSHVVPFGTDRAVHFHFHEVVSYWQEHPHRNYGIEVLIDSSQFSRRSPDANIFEDNGGMSALLRLENGFETRRKRNIEGGCRSDEGLCCKFPHRVDFEEIGLHWIVYPKSFFTHQCVGRCPESSIMSLAVNDVAVIRSSLHMLNESIHPAPCCVPTKLSNLTIMYMTDGDSPVLNLYEYPDFIVEECGCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C9R2</accession>
    <name>A0A9Q1C9R2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Reverse transcriptase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HOLleu_15077</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Nanhai2018</strain>
            <tissue evidence="16">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C9R2"/>
    <dbReference type="OrthoDB" id="115435at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00303">
        <property type="entry name" value="retropepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09274">
        <property type="entry name" value="RNase_HI_RT_Ty3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000020">
        <property type="entry name" value="Transposon Tf2-6 polyprotein-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.10.10:FF:000004">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048270">
        <property type="entry name" value="PNMA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041577">
        <property type="entry name" value="RT_RNaseH_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14893">
        <property type="entry name" value="PNMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17919">
        <property type="entry name" value="RT_RNaseH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="9" id="KW-0229">DNA integration</keyword>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="3" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <keyword evidence="12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="14">
        <location>
            <begin position="341"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase" evidence="15">
        <location>
            <begin position="704"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="239"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="285"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="942"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1112"/>
            <end position="1146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ8040704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1146" mass="127984" checksum="179FE6FD775FDBDC" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CAH9</accession>
    <name>A0A9Q1CAH9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_11869</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CAH9"/>
    <dbReference type="OrthoDB" id="6077919at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="2"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8041136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="269" mass="30617" checksum="9A3A5954696038D5" modified="2023-09-13" version="1">MYECRACNLTFRRSSNLRRHQRTSCPSFGHQRTEVCSGCGGQFYRRDVRQRHEGRCRGTGREHDQTERVRTRALQSEAPVGQHQTESAPDAILEVFVDPTEDLALEMQNRRQASTQTSAENSPPGNLQLRRGEQVLVRGRSYTLLTDCFLCVAPDDITLTEQATQTTETSGLDFNERWSLRTTLVGFDTEGNPLSRSEVRHEYSCPVRTLRSYGGPYNQFFVPPEYAPHPPFGGIIPPGASLLPRPPMANQRGYEEMFRRETERHARGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CAS8</accession>
    <name>A0A9Q1CAS8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">HAUS augmin-like complex subunit 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_12774</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CAS8"/>
    <dbReference type="OrthoDB" id="661220at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070652">
        <property type="term" value="C:HAUS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051011">
        <property type="term" value="F:microtubule minus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007098">
        <property type="term" value="P:centrosome cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051225">
        <property type="term" value="P:spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029327">
        <property type="entry name" value="HAUS4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026214">
        <property type="entry name" value="HAUS4_met"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16219">
        <property type="entry name" value="AUGMIN SUBUNIT 4 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16219:SF1">
        <property type="entry name" value="HAUS AUGMIN-LIKE COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14735">
        <property type="entry name" value="HAUS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02090">
        <property type="entry name" value="HAUSAUGMINL4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="294"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8041852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="371" mass="42891" checksum="6F39F6AD7E148692" modified="2023-09-13" version="1">MEPRSADKADGAIQVNMSLPTHLMGIDAENYPGLTDLLKSLSKHITKDGLSKSLKHDVEQAKENLEKEKHSYLQQHILHYELHEMMVDQEFAQQDLAPDSQTEQYHNALRECVSTAEACDYLHKRPYGQGDKTTSLLGLTEEDLLKSNPHMKKLPVLQQHYIPELEKRLKHKCEEVASCHFTPSDTEGSEQLAFAKATRLPDILKQECARLEEEKEKSRLDKALISKQFWQLYYALTEALAILEELITKHRLTLQAEKDSVTTDWLSARCHAMHLKIRVFRTKLLCDMYTPDAVMALQQIREHLSQTIQEKESELSRIDQALQAYRSIGMGFEELVQEYEQLQKAVTSKKWALKELKQSMTEEETQDWRVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CB69</accession>
    <name>A0A9Q1CB69_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Macrophage mannose receptor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_12371</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CB69"/>
    <dbReference type="OrthoDB" id="6369810at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050111">
        <property type="entry name" value="C-type_lectin/snaclec_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22803">
        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0675">Receptor</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C-type lectin" evidence="2">
        <location>
            <begin position="74"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="2">
        <location>
            <begin position="198"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="2">
        <location>
            <begin position="325"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="2">
        <location>
            <begin position="456"/>
            <end position="570"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8041528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="574" mass="65642" checksum="E676A540908AE859" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CEN9</accession>
    <name>A0A9Q1CEN9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_10320</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CEN9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040278571" evidence="1">
        <location>
            <begin position="19"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8043295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="196" mass="22678" checksum="84615B8512E440AC" modified="2023-09-13" version="1">MKMMKFFLVLLSCGLILGQRSPLRTIARAPLLQGRHSALTCRVSNEEFNNVQSISWEDLNTETLLKNDDFIESSVSEDPAGTREEMTVNATADFVRGRFHPFNFYYHQNPCYTTATYERLTSAINVGGRRVTVLFPDNFQQIKCRNVSCHKCPGKCVQKYKFEMALVRDRCRVRWDRVKVPSGCCCHHYLPYFFLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CGP9</accession>
    <name>A0A9Q1CGP9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">G2/M phase-specific E3 ubiquitin-protein ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_07918</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CGP9"/>
    <dbReference type="OrthoDB" id="512616at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15669">
        <property type="entry name" value="ePHD_PHF7_G2E3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034732">
        <property type="entry name" value="EPHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051188">
        <property type="entry name" value="PHD-type_Zinc_Finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059102">
        <property type="entry name" value="PHD_PHF7/G2E3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042013">
        <property type="entry name" value="PHF7/G2E3_ePHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12420:SF42">
        <property type="entry name" value="G2_M PHASE-SPECIFIC E3 UBIQUITIN-PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12420">
        <property type="entry name" value="PHD FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26054">
        <property type="entry name" value="PHD_G2E3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13771">
        <property type="entry name" value="zf-HC5HC2H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51805">
        <property type="entry name" value="EPHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="12">
        <location>
            <begin position="52"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="11">
        <location>
            <begin position="191"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="27"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8045003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="340" mass="38570" checksum="8C4E8B7E8D8086E9" modified="2023-09-13" version="1">MESEGPSKSLEEGNVEPDSSFEKINEKGNSSPSSGNNGISEESKTHQDKQIENVCIFCQLNEDNEFKYGKLYRLEDADNGRMITAHHYCLFFASGLCQKGSDNEGIQGFLANDIQKEVSRASRLKCSFCMKKGASVGCCIKKCRQCYHYPCGRDNGSLFRFYDDFVSFCSTHRPLQEFSTAEKMNLSLVKCPVCLEEVPNEPGFDVLKTPCCHNTFLHRDCIQQQALASGYYFFRCPICNNQKEFRDEMRSFGIHVPEQDAAWEREEGAFQELYQRHNKCDMEDCVCPKGNEHATTQGKWQIVLCDFCGSVGCHLECGRLERPSQDWACQSCRGMLSTGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CGZ3</accession>
    <name>A0A9Q1CGZ3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_07339</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CGZ3"/>
    <dbReference type="OrthoDB" id="10468622at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8044558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="247" mass="28136" checksum="239CB04D083D4E45" modified="2023-09-13" version="1">MDREHARQMRIMEADVQEVKNELRSLELQKKPAGIAKRVSNQNFNQPVLEVEKRVKISNDESSSSTPLLDQRHKMSITETIYYLSFQARQRFMDQAGVRARHDDEKQGTTKSLGETGIADKSGSVKSTANNPPVRTSAALWGILRDDEVNRTKSNRDALPSWKRGGMEERKVHTPCGRCNECIQKMRSRRDHVMQQLYGQGRHIAERMERRRLVSGSAGWSALKSVVIINNALKKTHQKEMVSLPGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CHG2</accession>
    <name>A0A9Q1CHG2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Testis-specific serine/threonine-protein kinase 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HOLleu_08433</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Nanhai2018</strain>
            <tissue evidence="18">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CHG2"/>
    <dbReference type="OrthoDB" id="504170at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050321">
        <property type="term" value="F:tau-protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF102">
        <property type="entry name" value="TESTIS-SPECIFIC SERINE_THREONINE-PROTEIN KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="9" id="KW-0221">Differentiation</keyword>
    <keyword evidence="8 18" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 14 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 15" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="13" id="KW-0744">Spermatogenesis</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="12" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="47"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ8045422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="311" mass="35088" checksum="40C2D811452D92DC" modified="2023-09-13" version="1">MAQANPSTPTNVPTPPGVRYHRTSTDRNSGQCDRHSCDKEVLCAKGYVIRESLGHGSYSKVKLAVLKSNVGARFAIKIIDRRRAPQDYQKNFLPRELATVRTLRHPYIIETYDVFEQNNKVYMILEYAQEGDVLDYVRFRSQGFVGEALAKRWVAQTAHALMYMHARSIAHRDVKCENLLLSTGKNIKLSDFGFVRHVSGNELSHTYCGSAAYAAPEIIRSTPYNPFLTDVWALGVVLFIISTGLMPFGEDVKDVSRILQIQLAGIQFPTAPRHRVSTDCQLLIRGMLAVNARQRFTLPQILSDKWLSKTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-06-18" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CI70</accession>
    <name>A0A9Q1CI70_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">Chromatin-remodeling ATPase INO80</fullName>
            <ecNumber evidence="11">3.6.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HOLleu_08857</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Nanhai2018</strain>
            <tissue evidence="16">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">ATPase component of the INO80 complex which remodels chromatin by shifting nucleosomes and is involved in DNA repair.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the INO80 chromatin-remodeling complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">The DBINO region is involved in binding to DNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.-" evidence="11"/>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="448448at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000006">
        <property type="entry name" value="Putative DNA helicase INO80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020838">
        <property type="entry name" value="DBINO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050520">
        <property type="entry name" value="INO80/SWR1_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45685:SF2">
        <property type="entry name" value="CHROMATIN-REMODELING ATPASE INO80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45685">
        <property type="entry name" value="HELICASE SRCAP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13892">
        <property type="entry name" value="DBINO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51413">
        <property type="entry name" value="DBINO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 11" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9 11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DBINO" evidence="15">
        <location>
            <begin position="322"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="13">
        <location>
            <begin position="559"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="14">
        <location>
            <begin position="1185"/>
            <end position="1341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="212"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1360"/>
            <end position="1515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1544"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="213"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="242"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="269"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="282"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1360"/>
            <end position="1374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1384"/>
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        </location>
    </feature>
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        <location>
            <begin position="1423"/>
            <end position="1438"/>
        </location>
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            <begin position="1497"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1545"/>
            <end position="1565"/>
        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1574"/>
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        </location>
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            <dbReference type="ARBA" id="ARBA00004123"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00019805"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU368001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51413"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAJ8045776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1599" mass="182930" checksum="D59F082E7D75E755" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CJ74</accession>
    <name>A0A9Q1CJ74_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_04982</name>
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    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
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            <taxon>Metazoa</taxon>
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            <taxon>Holothuroidea</taxon>
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            <taxon>Aspidochirotida</taxon>
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            <taxon>Holothuria</taxon>
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        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
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        <property type="match status" value="1"/>
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        <location>
            <begin position="1"/>
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            <begin position="1"/>
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            <begin position="18"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAJ8046342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="126" mass="14498" checksum="601AD60ADBDAE6CD" modified="2023-09-13" version="1">MDDKLKHPPSKSEDTVPKSGTNDPSRTNLIEHDVDVGDAAPIKQHPYRVNPRKRQHLRDEVRYMLDHDTIEPSNSEWSSPCILVPKPDVIRSWNQFLRYSTLQVYYCTIVDVHALPALGPDQNNIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CJA3</accession>
    <name>A0A9Q1CJA3_HOLLE</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ORF">HOLleu_08519</name>
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    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
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            <taxon>Eukaryota</taxon>
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            <taxon>Holothuriidae</taxon>
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        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="KAJ8045499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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            <begin position="20"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAJ8045499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="122" mass="13915" checksum="1A480A1EA36A808B" modified="2023-09-13" version="1">MLQAEQGRQGVPPGHMSDMPPVPPPPPMPPQQFLAQEERIEREKQRIFQQQQQLIQKRQEEQQRLEAEKMQKALQQQQMLAAEKMMEQGMGGPPPPPPGAPPGPPPPKLDEMMQEKVMQQQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CJA4</accession>
    <name>A0A9Q1CJA4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Cytochrome P450 2U1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_04991</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CJA4"/>
    <dbReference type="OrthoDB" id="1844152at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000004">
        <property type="entry name" value="cytochrome P450 2D15 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050182">
        <property type="entry name" value="Cytochrome_P450_fam2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300">
        <property type="entry name" value="CYTOCHROME P450 508A4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300:SF417">
        <property type="entry name" value="CYTOCHROME P450 508B1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0349">Heme</keyword>
    <keyword evidence="5 7 8" id="KW-0408">Iron</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="7">
        <location>
            <position position="443"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8046346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="498" mass="57084" checksum="02A9BAC992EE6E2D" modified="2023-09-13" version="1">MVLAALLSLVLSNLQVTLLFLIVFLVCFWWIRIPKNLPPGPFPLPIIGNSLKLFGKSNYELFCHLAEEYGSIYTFYLGNIRGIIVSDLNIIKEAAVKQADTFSNRFLPPILEWTVQDHGSVFFQNGPVWKERRKFFMQALRNFGVGKKSLEYKINEEACYMIKLFEEQEEKPFDPSQFTNYVIANVICHITFGKRFNYDDTDFQKIIQLLQTFNRSVSLGGVLQVFPWLVRTPFYRHVADVMDALKDFVTVYLNDHKETLNEDNHRDIIDMYLSEIKRQQQSGEEVIFKPEEAWRSIGELFSGGADSTTNTLLFAILLSVVNPDVQDKVVQEEIDRVIGSSRQPSYEDRLRMPYCEAVLMEVQRFKPVAPLAPPHGVFKDAFLAGYYIPKGSQVFFNLCAVLHDPKVWKNPKSFDPSNFLSEDGKSVIKPENFIPFGIGRRICIGEHLAKMELFLFYTNLMQRFKFSLPEGDPTPSLVPVVGILGKPVPFRICATPRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CJA6</accession>
    <name>A0A9Q1CJA6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Steroid 17-alpha-hydroxylase/17,20 lyase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_08853</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CJA6"/>
    <dbReference type="OrthoDB" id="639466at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004508">
        <property type="term" value="F:steroid 17-alpha-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042446">
        <property type="term" value="P:hormone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042448">
        <property type="term" value="P:progesterone metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24289">
        <property type="entry name" value="STEROID 17-ALPHA-HYDROXYLASE/17,20 LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24289:SF20">
        <property type="entry name" value="STEROID 17-ALPHA-HYDROXYLASE_17,20 LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0349">Heme</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8045773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="379" mass="42298" checksum="4D446841DBC86EB1" modified="2023-09-13" version="1">MLGLAESSGVVANSTSLVLVSAATALVGLWVWSQQKPRKDFPPGPKGWPLIGNILEFMSDSPPHIIFLEHAKKYGDIFSIRIGQRWTVVLNGVTVIKEALLKKGIEFANRPSTYSTDIITEGGKDISFGQFTPTWKLHRKLVYSAFRTLATGNNERFENLVYSILPGLTKHLESKGSEPFNPKPTLASSIYNILATMCFGKQFEFDGPEVKFWMRLSQEGIEVGGNGLAADYIPFMKYIPTPAVAKVKSLLKESSDAINRELEEHHASYDGGEPKDLIEMLLQVRQEMEDEGSEDMTMITETHIRQTVANIFSAGTDTSIFTMHWSVGLLVQFPEVQAKVAAELDKVVGRDRLPSLNDRNNLPYTAATLYEVMRWLHNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CJE9</accession>
    <name>A0A9Q1CJE9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Metalloproteinase inhibitor 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_05064</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protease inhibitor I35 (TIMP) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CJE9"/>
    <dbReference type="OrthoDB" id="6041373at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008191">
        <property type="term" value="F:metalloendopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002020">
        <property type="term" value="F:protease binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051045">
        <property type="term" value="P:negative regulation of membrane protein ectodomain proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.370.10">
        <property type="entry name" value="Tissue inhibitor of metalloproteinase-1. Chain B, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001134">
        <property type="entry name" value="Netrin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001820">
        <property type="entry name" value="TIMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008993">
        <property type="entry name" value="TIMP-like_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027465">
        <property type="entry name" value="TIMP_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11844">
        <property type="entry name" value="METALLOPROTEASE INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11844:SF33">
        <property type="entry name" value="TISSUE INHIBITOR OF METALLOPROTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00965">
        <property type="entry name" value="TIMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00206">
        <property type="entry name" value="NTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50242">
        <property type="entry name" value="TIMP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50189">
        <property type="entry name" value="NTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0481">Metalloenzyme inhibitor</keyword>
    <keyword evidence="4" id="KW-0483">Metalloprotease inhibitor</keyword>
    <keyword evidence="5" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040502047" evidence="10">
        <location>
            <begin position="21"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="NTR" evidence="11">
        <location>
            <begin position="21"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared with metalloproteinase partner</note>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="21"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="24"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="34"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="144"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="178"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR601820-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR601820-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8046406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="274" mass="31969" checksum="3EB342CB5B4B2D5D" modified="2023-09-13" version="1">MKTLLLFFLVGAVVVSQIEACGQCSTNHPQQHYCISDFAVRVEILQSKLDSTHEMKVIRARVIDNLKLSRNKKGKIIDFYTSSSFCGTVFYTGREYVITGSKETWRDGTKYLLHDSCDFGKEWNDLSPQQQKGFTRKYARYCECKIEPSRDATEIKVEDLSFENTYRGARTYWTPDNCYYNPHKSREFDDVEDCEEEYSYCAPGRGGECSWQLTPDYEECFTHRDDYVMVASSVSDGVYPELCIVLPRRRRKACRKRAREYAAQLEEIEILPFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CLV8</accession>
    <name>A0A9Q1CLV8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Histidine ammonia-lyase</fullName>
            <ecNumber evidence="4">4.3.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_05752</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-histidine = trans-urocanate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:21232"/>
            <dbReference type="ChEBI" id="CHEBI:17771"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
            <dbReference type="EC" id="4.3.1.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid degradation; L-histidine degradation into L-glutamate; N-formimidoyl-L-glutamate from L-histidine: step 1/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the PAL/histidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.3.1.3" evidence="4"/>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CLV8"/>
    <dbReference type="OrthoDB" id="10051290at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004397">
        <property type="term" value="F:histidine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006548">
        <property type="term" value="P:L-histidine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00332">
        <property type="entry name" value="PAL-HAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.275.10:FF:000008">
        <property type="entry name" value="Histidine ammonia-lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.200.10:FF:000003">
        <property type="entry name" value="Histidine ammonia-lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.200.10">
        <property type="entry name" value="Fumarase/aspartase (Central domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="entry name" value="Fumarase/aspartase (N-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001106">
        <property type="entry name" value="Aromatic_Lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005921">
        <property type="entry name" value="HutH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01225">
        <property type="entry name" value="hutH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006871">
        <property type="entry name" value="PRK09367.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10362">
        <property type="entry name" value="HISTIDINE AMMONIA-LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00221">
        <property type="entry name" value="Lyase_aromatic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="L-aspartase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0369">Histidine metabolism</keyword>
    <keyword evidence="8 10" id="KW-0456">Lyase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="638"/>
            <end position="658"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8046914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="658" mass="71950" checksum="04C8D28201430B34" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CMR6</accession>
    <name>A0A9Q1CMR6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phospholipid scramblase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_00861</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">May mediate accelerated ATP-independent bidirectional transbilayer migration of phospholipids upon binding calcium ions that results in a loss of phospholipid asymmetry in the plasma membrane.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the phospholipid scramblase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CMR6"/>
    <dbReference type="OrthoDB" id="191150at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017128">
        <property type="term" value="F:phospholipid scramblase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005552">
        <property type="entry name" value="Scramblase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025659">
        <property type="entry name" value="Tubby-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23248:SF63">
        <property type="entry name" value="PHOSPHOLIPID SCRAMBLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23248">
        <property type="entry name" value="PHOSPHOLIPID SCRAMBLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03803">
        <property type="entry name" value="Scramblase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54518">
        <property type="entry name" value="Tubby C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0564">Palmitate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="19"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU363116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8048512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="276" mass="30610" checksum="35077CFC40FB6628" modified="2023-09-13" version="1">MDPTTDTKYGLQSEADPAYVPPAPAPPPGQALPGQQPVVQWMPQPQSSLGCPPGLEYLTQLDQILVHQQVELLEAFSSWESKNKYQIKNVLGQQVYFAAEESEDCSRQCCGSDRSFVMHIVDNMNQEVIRAVRPFVGCCLFCWCADCSDDCAYAISVESPPGQTVGYVRQRASGWSPHYEIRDANSEVILKIRGPCCPYQGVGCTEDVDFSVLSADESHIVGKISKQWAGLAKEWYTNADNFGISFPKDLDVKAKATLLGALFLIDFMYFEDNNNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CPM9</accession>
    <name>A0A9Q1CPM9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tc1-like transposase DDE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_01659</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CPM9"/>
    <dbReference type="OrthoDB" id="8190933at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038717">
        <property type="entry name" value="Tc1-like_DDE_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13358">
        <property type="entry name" value="DDE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tc1-like transposase DDE" evidence="1">
        <location>
            <begin position="16"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8049083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="159" mass="17990" checksum="EDD604750D99CBA2" modified="2023-09-13" version="1">MAFRCTSKKSKVGSGGKVLRLYVAIAYGKGVVLAKPYKKLTGTKFAKFVQKEFPSLFERCGKTGSDRLFLQDGDPSQNSHRVARALRRINANVFKIPPSNPDLNPIENIFKLFGDKLNKDAIERNIKKLSNNFKQVLKKLYYRYPLTSPSELYAPGRSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CQM6</accession>
    <name>A0A9Q1CQM6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Nuclear pore complex protein Nup160</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_01694</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="67716at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059141">
        <property type="entry name" value="Beta-prop_Nup120_160"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021717">
        <property type="entry name" value="Nucleoporin_Nup160"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056547">
        <property type="entry name" value="NUP160_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056536">
        <property type="entry name" value="TPR_NUP160_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056535">
        <property type="entry name" value="TPR_NUP160_M"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21286">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21286:SF0">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11715">
        <property type="entry name" value="Beta-prop_Nup120_160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23345">
        <property type="entry name" value="NUP160_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23354">
        <property type="entry name" value="TPR_NUP160_120_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23347">
        <property type="entry name" value="TPR_Nup160_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Nucleoporin Nup120/160 beta-propeller" evidence="4">
        <location>
            <begin position="66"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="domain" description="NUP160 helical" evidence="5">
        <location>
            <begin position="568"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="domain" description="NUP160 middle TPR" evidence="7">
        <location>
            <begin position="837"/>
            <end position="1100"/>
        </location>
    </feature>
    <feature type="domain" description="NUP160 C-terminal TPR" evidence="6">
        <location>
            <begin position="1151"/>
            <end position="1407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF11715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8049103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1417" mass="158286" checksum="FE56724276952199" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CQP2</accession>
    <name>A0A9Q1CQP2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_02003</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CQP2"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8049311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="232" mass="26414" checksum="F10BF2061B4C07EB" modified="2023-09-13" version="1">MGNTQTSYLFALAAPNAADEVKHQSSNPPYSISTGADYVSTLSMLASLDKFKIIPVGYKSFDIQNACKQTLLSARSVKRRNTKGHAVRKAYDLTIAGAIVLVALEYENLQFQPNTSHLGYIHFHYKGIYDINVEIYDNTGNKIITTSTENRIHSMIYLDGQCFGSFQKRNSESGTKGQVLQHLDVRFKACILFAAVLACRYCHYYQDHHHHQLNNGHYNHAQHYHIHHIHRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CQV8</accession>
    <name>A0A9Q1CQV8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptidase A2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_02580</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CQV8"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF13">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="120"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="385"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="78"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="120"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="136"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="160"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="385"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="409"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="429"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="454"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8049713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="515" mass="56746" checksum="9AB0E7A762D5322D" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CRQ0</accession>
    <name>A0A9Q1CRQ0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_03249</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CRQ0"/>
    <dbReference type="OrthoDB" id="10066002at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31025">
        <property type="entry name" value="SI:CH211-196P9.1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31025:SF9">
        <property type="entry name" value="SI:DKEY-286J15.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="14"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8050156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="220" mass="25136" checksum="047A288F3EC68B3C" modified="2023-09-13" version="1">MAWFNPGSGKHDASGFLENRIRNVRRRDPAKSVETTPTTSKSSKMIDTINIDLSADDLDEANIAAMVTWLKYHNAPADQVKEMMGKTCKSRAAWIRKEKEKSVRELILEYPRLIDTDGMIDQDFRILFPDASNALFLKWTQNVQKLFDYGERQVNWKNLLKFSGKLDTDEQKSNLVLQMLPLLFPPGMKRRDGRKGPNVHATVAEASRAFIQVEKVKPVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CSA4</accession>
    <name>A0A9Q1CSA4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_03953</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CSA4"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040419956" evidence="1">
        <location>
            <begin position="27"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8050662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="55" mass="6548" checksum="E2CBE89915CF013E" modified="2023-09-13" version="1">MSYLRLTDKIFLILLSCLFNFKGTSTQEDHLISFTNNRNIQRTVKRFVISVRRVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CT39</accession>
    <name>A0A9Q1CT39_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_03852</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CT39"/>
    <dbReference type="OrthoDB" id="5982876at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8050590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="130" mass="14931" checksum="7DAD77CEB4ACAB8E" modified="2023-09-13" version="1">MKATGLLGEELSPETTLCSEHFEKDCFAIIRTGRRCLLKNSIRTKNLGQKRKKYDSVIPQTLSIKMFRIGQRVQAVDEIGRWEVGRVISIDNNGRPTVKFVGWEEDYNVQPLENEVRIPVDFYSHSAGKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CUQ5</accession>
    <name>A0A9Q1CUQ5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_04697</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8051214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CUQ5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="1">
        <location>
            <begin position="233"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8051214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="351" mass="40288" checksum="BD850DE217657C57" modified="2023-09-13" version="1">MSCFSSFSRTVKPAVPFPIIRSCGEVSNKCYAKRDDDDAFQCSIRGARPSIILEFMARTVEGDKDILEERRTIFENGEYGALATTREVFRYSSCLVLLVCKADAAPGLLEHNESLILVQNVPMEILGTKILYRYVERNRKIVLSCAKGDAAFVVWKKRSSFEYNVYEDLLYYVLIEEKFMEIFAENINVTRRGSLVIPRVEVKHEGMYVCVSGDGVSDSTIAYKLDVIVYPNPAYPVITGCSHRDYCELRRKYQGNLTCEVKGIRPQIKLEWKIPSQQENDKITFPTHQLTVEDNGETFDVTLISTYDIQDQSLDRMTVECNVLEPSHTKLKLSRKFDLIFMRGKQRLITR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1GZ43</accession>
    <name>A0A9Q1GZ43_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">3'-5' RNA helicase YTHDC2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_29064</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DEAD box helicase family. DEAH subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6103986at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.50:FF:000002">
        <property type="entry name" value="Immunoglobulin mu DNA-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1080:FF:000008">
        <property type="entry name" value="probable ATP-dependent RNA helicase YTHDC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000284">
        <property type="entry name" value="probable ATP-dependent RNA helicase YTHDC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.50">
        <property type="entry name" value="R3H-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001374">
        <property type="entry name" value="R3H_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036867">
        <property type="entry name" value="R3H_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059023">
        <property type="entry name" value="RNA_hel_CTD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF213">
        <property type="entry name" value="3'-5' RNA HELICASE YTHDC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01424">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26026">
        <property type="entry name" value="RNA_hel_CTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00393">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82708">
        <property type="entry name" value="R3H domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51061">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 14" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="R3H" evidence="11">
        <location>
            <begin position="12"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="12">
        <location>
            <begin position="186"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="9">
        <location>
            <begin position="493"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="13">
        <location>
            <begin position="597"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="95"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1172"/>
            <end position="1234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1266"/>
            <end position="1350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="95"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="108"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1174"/>
            <end position="1183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1281"/>
            <end position="1291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1326"/>
            <end position="1336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ8029622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1433" mass="159984" checksum="5BA72145B7CD45F3" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1GZL1</accession>
    <name>A0A9Q1GZL1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_29753</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8027720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1GZL1"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040417395" evidence="2">
        <location>
            <begin position="17"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8027720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="117" mass="13096" checksum="166DD4872F0A9BC0" modified="2023-09-13" version="1">MSATLVFLCLIAKISAARVETPTGNSSPLESKRAVADAREAPQLMTLQKKFEELEKKQESLMEEIRLLRETRQRTTQQKRMRRGASSGQGDMAPLPTNGKLLNIYMRLIGLSGTDNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H087</accession>
    <name>A0A9Q1H087_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_26686</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H087"/>
    <dbReference type="OrthoDB" id="6759844at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="14"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8030312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="107" mass="12444" checksum="8E37A94FCB5ACC6A" modified="2023-09-13" version="1">MFSSLFKKGYHRNNKKSSNVRYVERSNGESEITDAEDRQDSDNEYNYSEWAAPIDKPDGCYRIFGDYKVKVNVVLKTKENPLPTQQELLMKLNGGQKFTKLDTRISK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H0B5</accession>
    <name>A0A9Q1H0B5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Deleted in malignant brain tumors 1 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_32793</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H0B5"/>
    <dbReference type="OrthoDB" id="536948at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000001">
        <property type="entry name" value="Lysyl oxidase 4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000006">
        <property type="entry name" value="neurotrypsin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19331:SF465">
        <property type="entry name" value="EGG PEPTIDE SPERACT RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19331">
        <property type="entry name" value="SCAVENGER RECEPTOR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00530">
        <property type="entry name" value="SRCR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00258">
        <property type="entry name" value="SPERACTRCPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00420">
        <property type="entry name" value="SRCR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="SRCR" evidence="6">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="6">
        <location>
            <begin position="83"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="6">
        <location>
            <begin position="195"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="39"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="151"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="263"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8027601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="305" mass="33253" checksum="A92F2F641B58877C" modified="2023-09-13" version="1">MDDARVVCRQLGFRDAITAHGNAFFGEGEGPIHISNVNCDGTESFLKECYSSLSDNCEHEADASVTCLPFGKLLPHQSEEGFVRLVGGNLTDGRVEIFFKGEWGTICDDTWDISEATVVCRQLGFTGASSAPRKAAFGEGKGPIHLDTLECSGNELSLLDCEYKQKSNCMHNEDAGVECFNSTGVYFLTLDEGSVRLVGGHDMAGRVEIYKDNQWGTVCDDDWDLSDATIVCRQLGFKDAISAPRKAAFGEGVGPIHLDSVKCSGNEESLLDCEYKRDADCTHSEDASATCRNVSYGKYFEIRPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H171</accession>
    <name>A0A9Q1H171_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_29234</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H171"/>
    <dbReference type="OrthoDB" id="10445442at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040285614" evidence="2">
        <location>
            <begin position="35"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="139"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="139"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8029759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="301" mass="33764" checksum="40CB69B31C177575" modified="2023-09-13" version="1">MERLLRAPSRAATELCRYLLVSSLLLMRLCVITAEECDEIAKTGGMSVVQAFFSSKIEMHQQCEDLRRNLTQDCSESCNVAIEQFLENNGGELGRVFFSCECSEDSAMYTPEECEIDREKLVICPALYFMLQTDDLLTSTEAQEDTSPSKAPTTKEMPSTKPIIPPGKSCRKARHDCQKTLPCSKAFFTVKDFCLDDFTDGAQCSIDCRKAIDELDMFPAAEGLWGCGCDPDSDETTWCHMYGRQGLQKTCEGVKYKELNAAKTQENKDFKSDDPENSSKHHVISIFNCLLIPVLILIVVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H2B2</accession>
    <name>A0A9Q1H2B2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_26154</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H2B2"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8032592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="76" mass="8395" checksum="3D4628FEF543C206" modified="2023-09-13" version="1">MENLMCTEGAPRARSASPYAWGPGARVRAPGGSRGRSPRKLLQYCILRAQKSAPVLMSVRFYTFSCCMEFDVYGSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H6J7</accession>
    <name>A0A9Q1H6J7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_21550</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H6J7"/>
    <dbReference type="OrthoDB" id="6022531at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008528">
        <property type="term" value="F:G protein-coupled peptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009755">
        <property type="term" value="P:hormone-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24372:SF77">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24372">
        <property type="entry name" value="GLYCOPROTEIN HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="62"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="104"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="146"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="189"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="270"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="315"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="347"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="9">
        <location>
            <begin position="74"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8034633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="391" mass="43832" checksum="80BE508841A4B80E" modified="2023-09-13" version="1">MYKNLHFLFQKLFIDWFPFPLRLTDNYGICCLFANNNGTICTTPPSPFNTCGSMFPNHVLRATLWLVCLFAMLANLVVILSRIKAKIPAMRNMFATEVNTKQNAFLVSLAIADFFMGVYLLSIGIADAKFGEEYFLSAYGWRNGTWCKIIGSIGFVSNVASVLSLTFVSIERFVTVSFTFSGIRFGTKVTIGICVLIWIVSSVMAVTPIILSVFVQGLFGLSDVCLGLPFVTVPEDTETHVTVTYDNYGKFDSVIKNDPNDLQWLYSQIVYVYFTSTCVLIITVCYVAMFISIVRSRIGSGRRGNKDEIKTAIKMSIIVGTDMICWLPVIVFGILSKAGIEITIDMYAWIAIFVMPINSAFNPFIYTIPTIKKRKKNEPSFIADQRNFPAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H750</accession>
    <name>A0A9Q1H750_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">GATA zinc finger domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_19379</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8035639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H750"/>
    <dbReference type="OrthoDB" id="9994231at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039050">
        <property type="entry name" value="GATAD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13340">
        <property type="entry name" value="GATA ZINC FINGER DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13340:SF2">
        <property type="entry name" value="GATA ZINC FINGER DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="55"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8035639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="243" mass="27053" checksum="CF6F4A15F8242ED9" modified="2023-09-13" version="1">MLACSKMPYVPLRYVPLCYQCKTTVATVWRKIQGKTACNECYLKYKIKELAAAQGNGKGKDSSRISGRNSKKRKGKYNVGSVKGQATKGKNRRVIFKRNPTRSALSNATVVTGGSVFHEGIHYQVGDIVSLMDKDGALYFAQIRGLLQDLFCDKSAAITWLLPTQATSTDGFDPSSYILGPEEDVPRPLDEMHFVCHAPDDYFKAKYSPYAVMPNTEDKSFIWTSLTKADPPTVKEVFGSKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H7D5</accession>
    <name>A0A9Q1H7D5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Kielin/chordin-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_22256</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10045365at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19941">
        <property type="entry name" value="TIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.200.20">
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036084">
        <property type="entry name" value="Ser_inhib-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051216">
        <property type="entry name" value="Teneurin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002919">
        <property type="entry name" value="TIL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014853">
        <property type="entry name" value="VWF/SSPO/ZAN-like_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001007">
        <property type="entry name" value="VWF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001846">
        <property type="entry name" value="VWF_type-D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11219">
        <property type="entry name" value="TENEURIN AND N-ACETYLGLUCOSAMINE-1-PHOSPHODIESTER ALPHA-N-ACETYLGLUCOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11219:SF69">
        <property type="entry name" value="TENEURIN-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23106">
        <property type="entry name" value="EGF_Teneurin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01826">
        <property type="entry name" value="TIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00093">
        <property type="entry name" value="VWC"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23334">
        <property type="entry name" value="VWC2L_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00094">
        <property type="entry name" value="VWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00832">
        <property type="entry name" value="C8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="28"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00214">
        <property type="entry name" value="VWC"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SMART" id="SM00215">
        <property type="entry name" value="VWC_out"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00216">
        <property type="entry name" value="VWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57603">
        <property type="entry name" value="FnI-like domain"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57567">
        <property type="entry name" value="Serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="21"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01208">
        <property type="entry name" value="VWFC_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50184">
        <property type="entry name" value="VWFC_2"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51233">
        <property type="entry name" value="VWFD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1 4" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="106"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="157"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="348"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="428"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="464"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="591"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="682"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="735"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="814"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="867"/>
            <end position="947"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="1077"/>
            <end position="1108"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="1114"/>
            <end position="1207"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="1264"/>
            <end position="1342"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="1413"/>
            <end position="1507"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="1513"/>
            <end position="1544"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="1546"/>
            <end position="1577"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="1583"/>
            <end position="1677"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="1731"/>
            <end position="1808"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="1812"/>
            <end position="1843"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="1894"/>
            <end position="1968"/>
        </location>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="3605" mass="394931" checksum="791A643ED0BAD83E" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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    <name>A0A9Q1H8X3_HOLLE</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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    </gene>
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        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
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        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
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        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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            <begin position="1"/>
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        </location>
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAJ8036646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="159" mass="17335" checksum="036A48923156C8DB" modified="2023-09-13" version="1">MQSPTVGLQEAIATSDSEQDESTTPLADNQQTVEVVKLGGFATRFQAAPPTGPPLSEELATGIRGLISEKLDEAAIKEIMDKHVPPVNLDILEVPKVNPLIWENIPAKTKSKDLRLQKLQRPLVKGIVAVARCFPDNPTVEQQEALALLAQTNTEINMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
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        </submittedName>
    </protein>
    <gene>
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    <organism evidence="4 5">
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            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="term" value="C:nucleus"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8040527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="186" mass="20704" checksum="39BEC23E6D22C002" modified="2023-09-13" version="1">MSDSDDDLAEMRRLRESQKYKGGTTARDDSSHLHQERQGGEMSKEVKGTVPSDNETLTMAEMMGFSGFGKKAKSFDFTEMFEQAKRGAQERIQVSRDDSTKESEVKETEDKDDDSEEDDDDMIGPPLPPGFNLPSSSDETKKSNQKKDGDEDSDLDDDEDDNDIDLKSKIPASHEISLNHGAKSVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HBB6</accession>
    <name>A0A9Q1HBB6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phospholipase A2</fullName>
            <ecNumber evidence="8">3.1.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_16617</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1-acyl-sn-glycero-3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15801"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="5">Binds 1 Ca(2+) ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the phospholipase A2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.4" evidence="8"/>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HBB6"/>
    <dbReference type="OrthoDB" id="5841574at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047498">
        <property type="term" value="F:calcium-dependent phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050482">
        <property type="term" value="P:arachidonate secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00125">
        <property type="entry name" value="PLA2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.90.10">
        <property type="entry name" value="Phospholipase A2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001211">
        <property type="entry name" value="PLA2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016090">
        <property type="entry name" value="PLA2-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033112">
        <property type="entry name" value="PLA2_Asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033113">
        <property type="entry name" value="PLA2_histidine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036444">
        <property type="entry name" value="PLipase_A2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11716:SF51">
        <property type="entry name" value="PHOSPHOLIPASE A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11716">
        <property type="entry name" value="PHOSPHOLIPASE A2 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00068">
        <property type="entry name" value="Phospholip_A2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00389">
        <property type="entry name" value="PHPHLIPASEA2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00085">
        <property type="entry name" value="PA2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48619">
        <property type="entry name" value="Phospholipase A2, PLA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00119">
        <property type="entry name" value="PA2_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00118">
        <property type="entry name" value="PA2_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0106">Calcium</keyword>
    <keyword evidence="3 6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040544777" description="Phospholipase A2" evidence="8">
        <location>
            <begin position="17"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipase A2-like central" evidence="9">
        <location>
            <begin position="23"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="69"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="133"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="50"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="65"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="72"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="82"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="118"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR601211-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR601211-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR601211-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8039031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="158" mass="17912" checksum="5A9AC3CF411520F9" modified="2023-09-13" version="1">MKGLLVLFVLVGSGLAASRWKRDLLQFNEMVTCLTGRNAAWYYNGYGCWCGKGGNGNPVDETDQCCKDHDDCYKDLKSLGTCSTSWDLYTESYKYHYCTKNGDKKTECSRCTKRTVTCDSTNNACEMGLCECDKAAVECFQRAQYNGQYRVHVNLWNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HCU3</accession>
    <name>A0A9Q1HCU3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NLR family CARD domain-containing protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_12135</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HCU3"/>
    <dbReference type="OrthoDB" id="427518at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312">
        <property type="entry name" value="NACHT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312:SF2">
        <property type="entry name" value="NUCLEOTIDE-BINDING OLIGOMERIZATION DOMAIN-CONTAINING PROTEIN 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NACHT" evidence="1">
        <location>
            <begin position="411"/>
            <end position="534"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8041340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="848" mass="98276" checksum="AE5674EF7CF94C52" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HD96</accession>
    <name>A0A9Q1HD96_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Anamorsin homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Fe-S cluster assembly protein DRE2 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_11775</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery. Required for the maturation of extramitochondrial Fe-S proteins. Part of an electron transfer chain functioning in an early step of cytosolic Fe-S biogenesis, facilitating the de novo assembly of a [4Fe-4S] cluster on the cytosolic Fe-S scaffold complex. Electrons are transferred from NADPH via a FAD- and FMN-containing diflavin oxidoreductase. Together with the diflavin oxidoreductase, also required for the assembly of the diferric tyrosyl radical cofactor of ribonucleotide reductase (RNR), probably by providing electrons for reduction during radical cofactor maturation in the catalytic small subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="10">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="10">The C-terminal domain binds 2 Fe-S clusters but is otherwise mostly in an intrinsically disordered conformation.</text>
    </comment>
    <comment type="domain">
        <text evidence="10">The N-terminal domain has structural similarity with S-adenosyl-L-methionine-dependent methyltransferases, but does not bind S-adenosyl-L-methionine. It is required for correct assembly of the 2 Fe-S clusters.</text>
    </comment>
    <comment type="domain">
        <text evidence="10">The twin Cx2C motifs are involved in the recognition by the mitochondrial MIA40-ERV1 disulfide relay system. The formation of 2 disulfide bonds in the Cx2C motifs through dithiol/disulfide exchange reactions effectively traps the protein in the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the anamorsin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HD96"/>
    <dbReference type="OrthoDB" id="311633at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000085">
        <property type="entry name" value="Anamorsin homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03115">
        <property type="entry name" value="Anamorsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007785">
        <property type="entry name" value="Anamorsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049011">
        <property type="entry name" value="Anamorsin_N_metazoan"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046408">
        <property type="entry name" value="CIAPIN1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13273">
        <property type="entry name" value="ANAMORSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13273:SF14">
        <property type="entry name" value="ANAMORSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20922">
        <property type="entry name" value="Anamorsin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05093">
        <property type="entry name" value="CIAPIN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="3 10" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 10" id="KW-0408">Iron</keyword>
    <keyword evidence="8 10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Anamorsin N-terminal" evidence="12">
        <location>
            <begin position="8"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="Anamorsin C-terminal" evidence="11">
        <location>
            <begin position="264"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Fe-S binding site B" evidence="10">
        <location>
            <begin position="269"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Cx2C motif 1" evidence="10">
        <location>
            <begin position="269"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Cx2C motif 2" evidence="10">
        <location>
            <begin position="280"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="244"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="246"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="269"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="272"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="280"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF05093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF20922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8041068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="308" mass="33517" checksum="77A6336D19CD67A3" modified="2023-09-13" version="1">MTSAVESGDTVLLVWCEGHSPERIKELVERLQEKVGSNGQVRVENLQRLTQSAHPDSTFDRAVLGALSPVQTIHSDEVLGELVRVLRPNGRLHIKEPSSSAENGSSLRTKERLISSLKLSGFVDISEASIHKPNEEEGEAISKLYNIDKEKIQMVEVTCKKPNYEVGSISTLSLTLPKQRKLDIKPVSSDISKVWTLSSADMGDEDIDFIDEDDLLEDEDLLKPSADSLKADCGPGKKKACKNCTCGLAEELENGVQKSTKPKPATSACGNCYLGDAFRCASCPYLGMPAFKPGEKVALSSRQLNADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HDL8</accession>
    <name>A0A9Q1HDL8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_12116</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HDL8"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8041326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="102" mass="12270" checksum="5DDBE59C1064B4FA" modified="2023-09-13" version="1">MALFGIEHISSKPQCIFAFWFSYKSWFQRYEIFLYHMQYFRLLIHTVYSTQSCRHLFLRVRHVFVSCFLQFLCGRGMDNAPPHPLCLTLKCNHQVLVFCVEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HE49</accession>
    <name>A0A9Q1HE49_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_04840</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HE49"/>
    <dbReference type="OrthoDB" id="9996999at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053134">
        <property type="entry name" value="RNA-dir_DNA_polymerase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24559:SF444">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24559">
        <property type="entry name" value="TRANSPOSON TY3-I GAG-POL POLYPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8046227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="84" mass="9642" checksum="0B90CE802223C8BF" modified="2023-09-13" version="1">MLDNDLISPSQSPWASPILLVPKEDGSKRLCIDCRKVKVTMTDSHPIPRLDDCIDRIGNSDFVSKYHLLKGYWQVPLTQRARDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HF22</accession>
    <name>A0A9Q1HF22_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cilia- and flagella-associated protein 251</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_11274</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HF22"/>
    <dbReference type="OrthoDB" id="4899631at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000427">
        <property type="entry name" value="WD repeat domain 66"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050630">
        <property type="entry name" value="WD_repeat_EMAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13720:SF13">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 251"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13720">
        <property type="entry name" value="WD-40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="27"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="60"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="146"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="171"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="182"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="226"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00040994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8043949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1153" mass="127706" checksum="312BF0FE0D84FCBC" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HGB5</accession>
    <name>A0A9Q1HGB5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">G patch domain-containing protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_08430</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HGB5"/>
    <dbReference type="OrthoDB" id="6095487at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000467">
        <property type="entry name" value="G_patch_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051189">
        <property type="entry name" value="Splicing_assoc_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14195">
        <property type="entry name" value="G PATCH DOMAIN CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01585">
        <property type="entry name" value="G-patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00443">
        <property type="entry name" value="G_patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50174">
        <property type="entry name" value="G_PATCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G-patch" evidence="2">
        <location>
            <begin position="540"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="109"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="165"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="186"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="231"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="273"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8045419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="587" mass="65209" checksum="835267E57858892B" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HHC2</accession>
    <name>A0A9Q1HHC2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">F-box/WD repeat-containing protein 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_07916</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HHC2"/>
    <dbReference type="OrthoDB" id="19711at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050995">
        <property type="entry name" value="WD-F-box_domain-protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14604:SF4">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14604">
        <property type="entry name" value="WD40 REPEAT PF20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63829">
        <property type="entry name" value="Calcium-dependent phosphotriesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="F-box" evidence="4">
        <location>
            <begin position="52"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="176"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="216"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="256"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="296"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="336"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="376"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8045001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="489" mass="54465" checksum="9D880129D0E0C494" modified="2023-09-13" version="1">MRNIDEVLSILQENFDELSDEDQVAVLQKLILKCSLPQLKTLYLTLQPLIAVDFSHYLPQELISKIFGYLSAEDLSNVACCSKQWRDIANCDVLWHRLCENKGWLHYGTDHDLSSERPFSPSESSSVISSPTFVEENLIHFPSVLSQTCRWKETYIRASHLDKNWETGCYKVAPRLRGHKGKVTCLDSDGQVIVSGSMDSTVRVWDIFTGTCLHILEGHTDSVTCLQLLGSEIITGCADSVIRVYNVSSARCLKKLTGHEEGILRLKCQGGYLVTSAGDKTLRVWSWPSGKCQHTLRGHLDDIETFSLHNGLALSTSWDATCKVWDLAKGICMQTLKGHSEVVFCCQFDMVKAVTGGGDSLVKIWSLTSGTCTHTLEGHKDDVYCLQYDDDVIASGSADSSVRLWNHSGECLREMLEHIGVVRCLSLSNGRLVSGGDRKKIVVWDGKTGDLLNVVHRNPNLLHLMWVSDTKLITASPESPGVITLLSYW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HLE0</accession>
    <name>A0A9Q1HLE0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Interferon-induced transmembrane protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_02527</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CD225/Dispanin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HLE0"/>
    <dbReference type="OrthoDB" id="5989802at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051423">
        <property type="entry name" value="CD225/Dispanin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007593">
        <property type="entry name" value="CD225/Dispanin_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14948">
        <property type="entry name" value="NG5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14948:SF44">
        <property type="entry name" value="PROLINE-RICH TRANSMEMBRANE PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04505">
        <property type="entry name" value="CD225"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="49"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="97"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8049681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="126" mass="13745" checksum="F067C2ABE57CA3D2" modified="2023-09-13" version="1">MADTSWQFENDKQQILPPAYSQGSYDANPPIQTQTVIHINNAVPAPPDYFVFSLLVTIFCCLPFGIVGLVKSSEVRTRAAMGDSDGALQSSLSAKKWAYAGLITGLAIFGAVILFYVLLFVIVYSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0Y9H0</accession>
    <name>A0A9Q0Y9H0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_43522</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000343">
        <property type="protein sequence ID" value="KAJ8018472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0Y9H0"/>
    <dbReference type="OrthoDB" id="10029313at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21301">
        <property type="entry name" value="REVERSE TRANSCRIPTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21301:SF10">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8018472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="265" mass="30894" checksum="5A691446477891E0" modified="2023-09-13" version="1">MGPHREHIRGPPPLRHPLLHKRDWTPNVNRNRYIDCFTDSVEQHLRDFLNEVNHLSGSKIDNLSSQERQALRELRSKEYIVIKPADKGAAIVLQNREDFISEAHRQLADNSFYSPQHKIYSLLEQVVLDSEKPINDGNVIHLARKYCITPPGRPIVSGINTPTEYLSAYIDSFLQPLLKSIPSYIQDTTHFLRCLREIPYIQEGSFLVSLDVSSLYTNIPHEEGTKACREFLLKHHSPSRTNELCFLIEFILKNNYFQFLDRFYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YAC5</accession>
    <name>A0A9Q0YAC5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_42640</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the IFIT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000105">
        <property type="protein sequence ID" value="KAJ8019028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YAC5"/>
    <dbReference type="OrthoDB" id="10043504at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10271">
        <property type="entry name" value="INTERFERON-INDUCED PROTEIN WITH TETRATRICOPEPTIDE REPEATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10271:SF0">
        <property type="entry name" value="INTERFERON-INDUCED PROTEIN WITH TETRATRICOPEPTIDE REPEATS 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8019028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="892" mass="104436" checksum="062B83AB0A385E8B" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YAE5</accession>
    <name>A0A9Q0YAE5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_44858</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010001348">
        <property type="protein sequence ID" value="KAJ8017610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YAE5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="34"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8017610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="132" mass="15335" checksum="EEB27C32150704C6" modified="2023-09-13" version="1">MQLNNKEVEIEKKHIEERHEQTGRTERTRCKKAAFAFVKISNMLFASSFFSSLFVTLSLSLSTGMSVINSIPEDVFIDEMSTEDKQLYRQRDFIPSRNSNDLLWCGIILVKEYRGGKYAYFILPVALKQQNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YBN2</accession>
    <name>A0A9Q0YBN2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase A2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_43376</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000288">
        <property type="protein sequence ID" value="KAJ8018564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YBN2"/>
    <dbReference type="OrthoDB" id="8962223at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8018564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="219" mass="23381" checksum="421382B50025311F" modified="2023-09-13" version="1">MANCFTLAKLKETETASNALAIAENSSEVVVTLRPNSNDKIKEEYLPFVSEGFISLDGNSAHPPVKIKILRDTGATQSLLLDGVLPLSDSTSTGANVLIQGVECGFISVPLHKIDLKSDLVSGSVIVGVRPTLPVKGVSLLLGNDLAGGKVVANAILTDKPCDYNNTEQLEKEFPNLFPACAVTRAMSQKLAVDDNLNIHPPKVRIQFPKVEQVGNHVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YCJ5</accession>
    <name>A0A9Q0YCJ5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_42865</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000150">
        <property type="protein sequence ID" value="KAJ8018900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YCJ5"/>
    <dbReference type="OrthoDB" id="3263820at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46579">
        <property type="entry name" value="F5/8 TYPE C DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46579:SF1">
        <property type="entry name" value="F5_8 TYPE C DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8018900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="274" mass="31567" checksum="521A272C8FED1F61" modified="2023-09-13" version="1">MKVLLQKPVVYCKLGYRSVRNKESESSIEDIYDGEIHKELRQEGKPLSNDDNLSYTFNSDGVPVFTSSKYSLWPIFLMIYEPAPKMRRYNLILAGVWCGKTKPNMVFLQKFVEAQVLATDVVRWKRNGDEVVSRCFAVVSMHQQGLRCRIQCNTVQFNGYYGCGLRYHPDKLCGGVVKYPIDVVEYNDREEHEMLTDMQTALEENRSVRGIKGPTPLIAMSNFPVVWGFPPDFMHCCLLGVTRQLAELWFSTPGEDFHRKSKVYGIDRQATMHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YCP0</accession>
    <name>A0A9Q0YCP0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">2',5'-phosphodiesterase 12</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Mitochondrial deadenylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HOLleu_41576</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Nanhai2018</strain>
            <tissue evidence="16">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8019825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YCP0"/>
    <dbReference type="OrthoDB" id="412787at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004535">
        <property type="term" value="F:poly(A)-specific ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000288">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.10.10:FF:000018">
        <property type="entry name" value="2',5'-phosphodiesterase 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050410">
        <property type="entry name" value="CCR4/nocturin_mRNA_transcr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048821">
        <property type="entry name" value="PDE12-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121:SF37">
        <property type="entry name" value="2',5'-PHOSPHODIESTERASE 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121">
        <property type="entry name" value="CARBON CATABOLITE REPRESSOR PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21171">
        <property type="entry name" value="PDE12-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="2',5'-phosphodiesterase 12-like N-terminal" evidence="15">
        <location>
            <begin position="194"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="14">
        <location>
            <begin position="360"/>
            <end position="641"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00072755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00083541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF21171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ8019825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="651" mass="73489" checksum="AB3CD5DAB403E64C" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YDE2</accession>
    <name>A0A9Q0YDE2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Solute carrier organic anion transporter family member</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_40366</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the organo anion transporter (TC 2.A.60) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YDE2"/>
    <dbReference type="OrthoDB" id="5062115at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015347">
        <property type="term" value="F:sodium-independent organic anion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:monoatomic ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043252">
        <property type="term" value="P:sodium-independent organic anion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036058">
        <property type="entry name" value="Kazal_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004156">
        <property type="entry name" value="OATP"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00805">
        <property type="entry name" value="oat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11388">
        <property type="entry name" value="ORGANIC ANION TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11388:SF100">
        <property type="entry name" value="SOLUTE CARRIER ORGANIC ANION TRANSPORTER FAMILY MEMBER 4A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03137">
        <property type="entry name" value="OATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100895">
        <property type="entry name" value="Kazal-type serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51465">
        <property type="entry name" value="KAZAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="114"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="156"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="180"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="236"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="277"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="320"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="391"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="428"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="463"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="589"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="633"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="681"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="105"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="domain" description="Kazal-like" evidence="11">
        <location>
            <begin position="509"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="55"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="15"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="57"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8020707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="748" mass="82870" checksum="59AD2AEF6B32C363" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YHI9</accession>
    <name>A0A9Q0YHI9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Growth hormone secretagogue receptor type 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_37569</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YHI9"/>
    <dbReference type="OrthoDB" id="5864054at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00637">
        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24243">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24243:SF224">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 19-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="60"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="100"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="164"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="211"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="251"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="10">
        <location>
            <begin position="40"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8022615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="320" mass="35097" checksum="241B6D84A8C4361A" modified="2023-09-13" version="1">MENDTAIPEPEAEPEPHLGTCFQVTTTTIAALVITVGTIGNILVVLVVARMSIGRTVTDIFLASLAVADLLVCLACTPLLIVGVLRLNGHTGIHFKVEQILFYFSSVASILNLTAISIDRYDAVINATKRRLTTTIAYQLLVIIWATSALVALIIFLIPPKTEFGVLALVFVAPFITMVCIYRATLKKTRQVAKVATAGSKKSLSYRMDKTVRMVIIILVVFVVSWVPSLISRLLKYVLAHKASATSLSRMEVAACLIAYTGSALNFLVYAVMSRKFKMELYKLLGLSKYINRRVQSDNHSSMDIGKTQVVPAKSEDRTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YJ16</accession>
    <name>A0A9Q0YJ16_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transposable element P transposase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_35776</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YJ16"/>
    <dbReference type="OrthoDB" id="2441813at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048366">
        <property type="entry name" value="TNP-like_GBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048367">
        <property type="entry name" value="TNP-like_RNaseH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048365">
        <property type="entry name" value="TNP-like_RNaseH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012934">
        <property type="entry name" value="Znf_AD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21788">
        <property type="entry name" value="TNP-like_GBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21789">
        <property type="entry name" value="TNP-like_RNaseH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21787">
        <property type="entry name" value="TNP-like_RNaseH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51915">
        <property type="entry name" value="ZAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZAD" evidence="3">
        <location>
            <begin position="10"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="839"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="847"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8023362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="865" mass="98127" checksum="0C4601D002F8D1B1" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YJA1</accession>
    <name>A0A9Q0YJA1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Aminopeptidase Ey</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HOLleu_37220</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Nanhai2018</strain>
            <tissue evidence="18">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YJA1"/>
    <dbReference type="OrthoDB" id="510539at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.50.20:FF:000001">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1910:FF:000006">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024571">
        <property type="entry name" value="ERAP1-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050344">
        <property type="entry name" value="Peptidase_M1_aminopeptidases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533:SF276">
        <property type="entry name" value="GLUTAMYL AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533">
        <property type="entry name" value="PROTEASE M1 ZINC METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11838">
        <property type="entry name" value="ERAP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 18" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M1 membrane alanine aminopeptidase" evidence="16">
        <location>
            <begin position="5"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="ERAP1-like C-terminal" evidence="17">
        <location>
            <begin position="190"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF11838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ8022350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="528" mass="60910" checksum="A84C9889CBCB802B" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YKH9</accession>
    <name>A0A9Q0YKH9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_36869</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8024208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YKH9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8024208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="71" mass="7866" checksum="9CCE79A669B8C18A" modified="2023-09-13" version="1">MINGEMFNSTPPVTKLNLQENRTFDSVSTLQFSPTGEEVNVTCNISLDGTDIKTSASATFLTYIYQVFSFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YLB8</accession>
    <name>A0A9Q0YLB8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein-histidine N-methyltransferase</fullName>
            <ecNumber evidence="3">2.1.1.85</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_34389</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the methyltransferase superfamily. METTL18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.85" evidence="3"/>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YLB8"/>
    <dbReference type="OrthoDB" id="1723750at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018064">
        <property type="term" value="F:protein-L-histidine N-tele-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019410">
        <property type="entry name" value="Methyltransf_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614">
        <property type="entry name" value="HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614:SF39">
        <property type="entry name" value="HISTIDINE PROTEIN METHYLTRANSFERASE 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10294">
        <property type="entry name" value="Methyltransf_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8024474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="291" mass="32862" checksum="61CA20511E2116E9" modified="2023-09-13" version="1">MDFQFNFSLGDFNRTDLETHDCREESSDQRSLPASEEILPEPQLCPEFLESEYEVLKLGKNIAINCLRSRSVEGRLGGLNDDNCIQKTMANHSDLIPNVYEGGLKVWECSLDLVQYLKEARVPLEGLHILDLGCGAGLPGIFALQQNAAQVCFQDFNKEVLNYLTIPNVRLNIGDRDISETCQFISGDWGQIGDDLIASNRFDVILTSETIYNTDSVPSLYTLMKRTLKPEGMILVAAKTHYFGVGGGTRLFEEYIQKQLDFRIEVCKKITEGVQREILQMKYKEKKVSDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YNB4</accession>
    <name>A0A9Q0YNB4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_34380</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YNB4"/>
    <dbReference type="OrthoDB" id="2142683at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050111">
        <property type="entry name" value="C-type_lectin/snaclec_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22803">
        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C-type lectin" evidence="1">
        <location>
            <begin position="99"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8024465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="193" mass="21224" checksum="B26B20C54666A275" modified="2023-09-13" version="1">MRVFERGLTSKRKTTTIEETTTVEETTTVEEKTIAEETTIAEETTTVEKTTTVEGTTAVEETIAVEETTTVEETTTVKETTIKPTTPNNSVAEVCQTIWGSSCYLYVSTSAPSWDAAAAQCLTYHDNAHLVFIGSASENDFVVQLVRSFKVPLIGLRDVGGEWVWSSQGTTVDVTVTYSKWKSGYNNREPGED</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YNU1</accession>
    <name>A0A9Q0YNU1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Prolow-density lipoprotein receptor-related protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_33503</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YNU1"/>
    <dbReference type="OrthoDB" id="10017719at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050685">
        <property type="entry name" value="LDLR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24270">
        <property type="entry name" value="LOW-DENSITY LIPOPROTEIN RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00261">
        <property type="entry name" value="LDLRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040282933" evidence="9">
        <location>
            <begin position="22"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="102"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8025838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="155" mass="17613" checksum="71A16DDCEB6D8133" modified="2023-09-13" version="1">MKKTSLLGLLVGLLCLSVGSAKKGSPKFYRKCSNFRQGENKFECKTNKGMCVSKVYFCDSTVDCLDNSDEDPVVCEKEHKEYCDTGFEGKYINSTPLEKWQCDDKMCIEKCRRCDGDEDCPDGSDEDPERCADKTLKAELKAVDYQCQRRVHSEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YP12</accession>
    <name>A0A9Q0YP12_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">FGFR1 oncogene partner 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_36247</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SIKE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YP12"/>
    <dbReference type="OrthoDB" id="21214at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008555">
        <property type="entry name" value="SIKE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12186:SF2">
        <property type="entry name" value="FGFR1 ONCOGENE PARTNER 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12186">
        <property type="entry name" value="SIKE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05769">
        <property type="entry name" value="SIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="185"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="226"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="351"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="36"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="195"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="226"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="260"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="285"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="359"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="376"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8023727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="407" mass="45162" checksum="ADC4EE96F6AAB944" modified="2023-09-13" version="1">MSISIEQVLADAKRLVERLRVHDNAADSLIQQTTGLNKRIETMTEYQEEIEEMNNIARHRPRTALILGIQRENRQIRQLQEENAELVASLKQHQAALDLIMSSYREQITRIRNAHKSEQDLLQKHSSDTNVTSEYISKIIEMAAVMQKAADCDETYCFQMEETISKLKYENSGLRELLKITKSVTESAGRQPLPKSDKTSEGEGKSQALNETVILSSPKLGVWEELKENSASEETERQQERHASPDSPHVIRKPIATTCKKNEQKVKGKDSSGAISGKRNENKNNNHSANVKSRPGNLNQNKSVSGPNKASVGERLPDGVATSTSLTNCNRETKIATKGAISDPELALESEHLSDDFDTSSLSSQSSISTVKEVDSLSISSEISDSSGDLSEKMIDFSEINVDSTHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YQ02</accession>
    <name>A0A9Q0YQ02_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">E3 ubiquitin-protein ligase E3D</fullName>
            <ecNumber evidence="2">2.3.2.26</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">HECT-type E3 ubiquitin transferase E3D</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">UbcH10-binding protein with a HECT-like domain</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Ubiquitin-conjugating enzyme E2C-binding protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_31237</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome. Independently of its E3 ubiquitin-protein ligase activity, acts as an inhibitor of CPSF3 endonuclease activity by blocking CPSF3 active site.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Interacts with UBE2C/UbcH10 (E2 ubiquitin-conjugating enzyme). In vitro, interacts with cyclin-B.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="2"/>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YQ02"/>
    <dbReference type="OrthoDB" id="66510at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030332">
        <property type="term" value="F:cyclin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031624">
        <property type="term" value="F:ubiquitin conjugating enzyme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051865">
        <property type="term" value="P:protein autoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006513">
        <property type="term" value="P:protein monoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019193">
        <property type="entry name" value="UBQ-conj_enz_E2-bd_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31531:SF2">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE E3D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31531">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE E3D FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09814">
        <property type="entry name" value="HECT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00029737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00032234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00032298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00053831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00064185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8026425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="406" mass="46330" checksum="B8F7BBCB2480459E" modified="2023-09-13" version="1">MYGEVRKSLETAQLVFDVEHHVKEAPNSEGKCSNDFQSTGKICCQDLKCKFAITPSEVSIFNSVKDETTIFHLPPCLQLVPQSCSSANLVNQTELHLRIKVLDLKSVEIVNYEDEWTLTDGQQACIACQRCGNEITRKSLKKVIPMPSSDWEGVMEELWCCRSKDVAGVPPVLESIPEDDECLSGYFEIILSPTQLIWETVTIDDPQESAIPKKSSKVYKTVHCSRCRSCLGKALLADKKNEITAVHLWKCWTILHQNEITNIRPSSNLEYLVTSYIKDVCKQQVCFKFIVESEDHKAQILLWLVSPDSLLLWSDEPEMLKDERNRSARNMNIQCISIMKVLYQTCWDDKGQKMNDEWSHDMMVHSFILPLEACYQLAEILTQSTHCTPKSMRSTNSLMIGYLKTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YQ37</accession>
    <name>A0A9Q0YQ37_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NADPH oxidase 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_31216</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YQ37"/>
    <dbReference type="OrthoDB" id="167398at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043020">
        <property type="term" value="C:NADPH oxidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016175">
        <property type="term" value="F:superoxide-generating NAD(P)H oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042554">
        <property type="term" value="P:superoxide anion generation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050369">
        <property type="entry name" value="RBOH/FRE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11972">
        <property type="entry name" value="NADPH OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11972:SF153">
        <property type="entry name" value="SUPEROXIDE-GENERATING NADPH OXIDASE HEAVY CHAIN SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="34"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8026409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="112" mass="12880" checksum="4B46EEE6A72CD9EA" modified="2023-09-13" version="1">MGRSSEKPVSVRRTVPHTSCTYYQRMRNGHHIEVVFLVFWLFVNVAVFYATYQYYKHESKFFYLRDILGIGLCLSRGSASVLNLNSAALLLPMCRTILVKLRGSRVVSCNVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YSJ2</accession>
    <name>A0A9Q0YSJ2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">E3 ubiquitin-protein ligase XIAP</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_32200</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IAP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YSJ2"/>
    <dbReference type="OrthoDB" id="774873at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00022">
        <property type="entry name" value="BIR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1170.10:FF:000002">
        <property type="entry name" value="Baculoviral IAP repeat containing 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1170.10">
        <property type="entry name" value="Inhibitor Of Apoptosis Protein (2mihbC-IAP-1), Chain A"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001370">
        <property type="entry name" value="BIR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050784">
        <property type="entry name" value="IAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10044:SF139">
        <property type="entry name" value="DEATH-ASSOCIATED INHIBITOR OF APOPTOSIS 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10044">
        <property type="entry name" value="INHIBITOR OF APOPTOSIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00653">
        <property type="entry name" value="BIR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00238">
        <property type="entry name" value="BIR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57924">
        <property type="entry name" value="Inhibitor of apoptosis (IAP) repeat"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50143">
        <property type="entry name" value="BIR_REPEAT_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="7">
        <location>
            <begin position="738"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="671"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="689"/>
            <end position="701"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8027146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="785" mass="90585" checksum="858993ABD9F8D36F" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YSM3</accession>
    <name>A0A9Q0YSM3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">LIM domain-binding protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_33490</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YSM3"/>
    <dbReference type="OrthoDB" id="774557at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029005">
        <property type="entry name" value="LIM-bd/SEUSS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10378">
        <property type="entry name" value="LIM DOMAIN-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01803">
        <property type="entry name" value="LIM_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8025827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="88" mass="10338" checksum="D5D395E63D23AE47" modified="2023-09-13" version="1">MNQPDYRIYELNKRLQHRAEESDNLWWDAFATEFFEDDAILTLAFCLEDGPKRYSKIVFFSFCVTVHGAGFPATLESKENMKKMSFSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BA09</accession>
    <name>A0A9Q1BA09_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_39685</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BA09"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="8"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8020166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="95" mass="10818" checksum="61EC0A3C74B19EDA" modified="2023-09-13" version="1">MMQFQFTLFSAQRQKRGRPKQSKGQNEVTSWQQNKPLGNKYNCGRCGRKHGLKECPAIGTILVKRQVKRGTRSNFTHLGDELSPLDPPQEDVPHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BAB2</accession>
    <name>A0A9Q1BAB2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_44224</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000722">
        <property type="protein sequence ID" value="KAJ8018018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BAB2"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8018018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="52" mass="5795" checksum="B5A051510AA77875" modified="2023-09-13" version="1">MNVILSMQMHYGKRKNPIVFGGGQRSFGVTGVKLKTLLTRYLDVGSMDKFCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BBE1</accession>
    <name>A0A9Q1BBE1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_43734</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000434">
        <property type="protein sequence ID" value="KAJ8018332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BBE1"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8018332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="64" mass="7682" checksum="2C70D79F1E18FD61" modified="2023-09-13" version="1">MRYGMRKNPIIFGGGERSSGVTGGQTLKTLLTRYLKKVGRLDEFHTLYVDALWYEEEPYYFWWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BC38</accession>
    <name>A0A9Q1BC38_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_37092</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BC38"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8022251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="79" mass="9212" checksum="E345C59C65262FDE" modified="2023-09-13" version="1">MLEQNEILNFQLTTHIHIHGFNGSRKTACNDRTVRVECHPLSVPRRQNLHLINRWTSRPVTSTSDRSQAARWAPRNIKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BCL2</accession>
    <name>A0A9Q1BCL2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptidase A2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_38894</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BCL2"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF13">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="177"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="422"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="78"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="178"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="196"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="430"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="465"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="490"/>
            <end position="504"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8021635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="585" mass="65645" checksum="B61127FC4D549599" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BDQ4</accession>
    <name>A0A9Q1BDQ4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">ATP-dependent DNA helicase PIF1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_38641</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BDQ4"/>
    <dbReference type="OrthoDB" id="10069494at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051055">
        <property type="entry name" value="PIF1_helicase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47642">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0347">Helicase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8021439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="139" mass="15575" checksum="DE6E21E5059E8D4A" modified="2023-09-13" version="1">MIPTLELSTEKNTPTTQFTPCNHSLAATQRSLLHQNTSCDRPQYPLKLAWALTIHKVQGMTMTKATVSLKCIFEPGMKHFVFSCARSVQCLNIYGFQPINCDKRVQDALTKTVDCDMLSSNKLLHPPTSVNFSIVHHNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BF95</accession>
    <name>A0A9Q1BF95_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SCAN box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_35699</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BF95"/>
    <dbReference type="OrthoDB" id="10051775at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF1">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8023313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="226" mass="26257" checksum="780CB93568FA2F2D" modified="2023-09-13" version="1">MERYQNYVKIGQELGLTGKDLLDFANEKDQKDRESENAAREERRKEREHEITLKEQDVKIAEKKASEQTDASGSQNTKSVQKPRLPKLQSFSEVTDDLDAYIQRFERFAKAAGWPDSEWAISLGALLTGRALETYSRLPLSDVNDYKKVKAALLIRYNLTEEGFREKFRNAQLHSKESYTQLGERLRGYVNRWVESAGKKKTYDDLLDIIVREQVLSVRICLYLLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BFA8</accession>
    <name>A0A9Q1BFA8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Fucosyltransferase</fullName>
            <ecNumber evidence="5">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_34385</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Golgi apparatus</location>
            <location evidence="5">Golgi stack membrane</location>
            <topology evidence="5">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the glycosyltransferase 10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="5"/>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BFA8"/>
    <dbReference type="OrthoDB" id="427096at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046920">
        <property type="term" value="F:alpha-(1-&gt;3)-fucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11660:FF:000004">
        <property type="entry name" value="Glycoprotein 3-alpha-L-fucosyltransferase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11660">
        <property type="entry name" value="Glycosyl transferase family 10, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055270">
        <property type="entry name" value="Glyco_tran_10_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001503">
        <property type="entry name" value="Glyco_trans_10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038577">
        <property type="entry name" value="GT10-like_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11929">
        <property type="entry name" value="ALPHA- 1,3 -FUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11929:SF145">
        <property type="entry name" value="ALPHA-(1,3)-FUCOSYLTRANSFERASE FUT-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00852">
        <property type="entry name" value="Glyco_transf_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="Fucosyltransferase C-terminal" evidence="6">
        <location>
            <begin position="262"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8024470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="482" mass="56137" checksum="BDBCF0FC39A92B16" modified="2023-09-13" version="1">MWAATPFAKLTVSYMGRIARLLILFLLLVIVVTFHFFVPIVNSNFRESSAPSGSTIAHSVSRTPKKNVSFVRDKTPRTQKSSAKYSQYLGERNDVWGRTLPADVQMAPRKTIVNVGFFEGYRKTWNYPTLFQPPLSPRICECFKENDVKYYANVTLETNTSRTGMYGILFFPQDVSVKQLEPHVWDAILMTSPHYQRRIYATFEGAAKLKFLALDPPYDKKVFHWSLTHHSKSEFTMPYGYYSPFSKQLSTLGPKKNWAGGRTGLVAWMSTWSVTTWKRDGFVNKLQKHISVDMFGERNGRKRCPRNSTECEQKIRKYKFYLALENSCCSEYISEKFWRTLLWDIVPIVVGAPRESYERLAPPHSFIYADDFVSIQDLAQYIFKLHKNDTLYNQYFEWKEKGEVFINYPDLHNTAVTSREPPKTFFYSCNSVCKVSRKYLDEKPDTVRNNGEKLNNSSFFDPKVSWWCGSCTKCGKHDWIRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BG17</accession>
    <name>A0A9Q1BG17_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Ubiquitin-NEDD8-like protein RUB2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_35633</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BG17"/>
    <dbReference type="OrthoDB" id="654191at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16449">
        <property type="entry name" value="RING-HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047153">
        <property type="entry name" value="TRIM45/56/19-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25462">
        <property type="entry name" value="BONUS, ISOFORM C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25462:SF296">
        <property type="entry name" value="MEIOTIC P26, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="19"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="5">
        <location>
            <begin position="101"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8023267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="188" mass="21360" checksum="D886903DEC562917" modified="2023-09-13" version="1">MAEELNLRDVENIQREADCSICLNKCSERLLPNCGHSFCEECLHKINENGKISCPECRKVSTLPDGKVQNLMRNFVAMRIRDQTTTIIEEKGRATGESAKMKLVVNLLNGKKMEVQVNGPDVTVNELKREIAEKSNIGEDHQRLLYLGKELENEKKLGHYKIGPYSTIHMVQRMLGGRLILFNILSYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BGT5</accession>
    <name>A0A9Q1BGT5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Mitotic checkpoint protein BUB3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HOLleu_33684</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Nanhai2018</strain>
            <tissue evidence="17">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Centromere</location>
            <location evidence="2">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the WD repeat BUB3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BGT5"/>
    <dbReference type="OrthoDB" id="10262475at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000047">
        <property type="entry name" value="Mitotic checkpoint protein bub3, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10971">
        <property type="entry name" value="MRNA EXPORT FACTOR AND BUB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="13" id="KW-0137">Centromere</keyword>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="9" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="11" id="KW-0469">Meiosis</keyword>
    <keyword evidence="7" id="KW-0498">Mitosis</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 16" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="16">
        <location>
            <begin position="93"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="16">
        <location>
            <begin position="238"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00037960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00040107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ8025970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="327" mass="36996" checksum="D6CFE5E7C1706A0E" modified="2023-09-13" version="1">MPDATSEFKLNDCPEDGISAVKFGPNSSQFLLVSSWDETVRLYDVQANTMRAKYKHDRAVLDCCFHDQTHVFSGGLDNTLKMYDINKNAEETVGKHDHAIRCVEFCPDVNVLVTGSWDQTVKLWDARISHCAGSFSQPDKVYTLALCGDRLVVGTANRKVLVWDLRNMGYVQQRRESSLKYQTRCIRCFPNKQGYVLSSIEGRVAVEYLDPSPEVQKKKYAFKCHRLKVNGEENIYPVLSIAFHNVHNTFATGGSDGYVNIWDGFNKKRLCQFHRYPSSISSLAFSNDGSTLAIASSYMYEQGADAEHPEDAIFIRNVSDQETKPKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BIM6</accession>
    <name>A0A9Q1BIM6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">(S)-2-hydroxy-acid oxidase</fullName>
            <ecNumber evidence="2">1.1.3.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_32397</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>2-hydroxyoctanoate + O2 = 2-oxooctanoate + H2O2</text>
            <dbReference type="Rhea" id="RHEA:67940"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:133514"/>
            <dbReference type="ChEBI" id="CHEBI:176689"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:67941"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a (2S)-2-hydroxycarboxylate + O2 = a 2-oxocarboxylate + H2O2</text>
            <dbReference type="Rhea" id="RHEA:16789"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="ChEBI" id="CHEBI:58123"/>
            <dbReference type="EC" id="1.1.3.15"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:16790"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.3.15" evidence="2"/>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BIM6"/>
    <dbReference type="OrthoDB" id="25826at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003973">
        <property type="term" value="F:(S)-2-hydroxy-acid oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001561">
        <property type="term" value="P:fatty acid alpha-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02809">
        <property type="entry name" value="alpha_hydroxyacid_oxid_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000056">
        <property type="entry name" value="hydroxyacid oxidase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012133">
        <property type="entry name" value="Alpha-hydoxy_acid_DH_FMN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000262">
        <property type="entry name" value="FMN-dep_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037396">
        <property type="entry name" value="FMN_HAD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578:SF149">
        <property type="entry name" value="2-HYDROXYACID OXIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578">
        <property type="entry name" value="S -2-HYDROXY-ACID OXIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01070">
        <property type="entry name" value="FMN_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000138">
        <property type="entry name" value="Al-hdrx_acd_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51349">
        <property type="entry name" value="FMN_HYDROXY_ACID_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0288">FMN</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FMN hydroxy acid dehydrogenase" evidence="9">
        <location>
            <begin position="1"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="278"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="80"/>
            <end position="82"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="276"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="278"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="281"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="310"/>
            <end position="314"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="333"/>
            <end position="334"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000138-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000138-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8027288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="389" mass="42596" checksum="21B9336212F1ECE9" modified="2023-09-13" version="1">MSPVLCLDDFRELARKKKSDTPTLFSYYETGAGDEQTLEDSIAAFKRFRLIPRLLRLQPNLDLKTTVQGHTIRVPFGIAPTAIQVTAHPDGEKATAKAAGKFGTIMIVSSISQTPIEEIVEAAPDTHLWMQTYIFKNRRNTIDLVKRAERLGFKGIVVTVDSPVIGEFKRAGKQYEDYSEEYDKKYSKEEGTVPFEVKGVSLANFRAAPEEIAAARASGDEGLFAYAREKIQSPVNWTDVTRLATMTRLPIIVKGVITAEGAREAVAAGVQGIIVSAHGGRQLDGIQAPIEALPAIIDAVRGFDVEVYMDGGVRSGRDIFKALAIGAKAVFIGRPIIWGLIVNGAQGVLDVLEMLENELKNTMALCGCNKVSEVTRSFVQHESQLICRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BKL0</accession>
    <name>A0A9Q1BKL0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">phosphatidylinositol-3,5-bisphosphate 3-phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.95</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_30559</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein-tyrosine phosphatase family. Non-receptor class myotubularin subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.95" evidence="4"/>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BKL0"/>
    <dbReference type="OrthoDB" id="271628at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052629">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate 3-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004438">
        <property type="term" value="F:phosphatidylinositol-3-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14535">
        <property type="entry name" value="PTP-MTM1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000038">
        <property type="entry name" value="Myotubularin 1, isoform CRA_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004182">
        <property type="entry name" value="GRAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030564">
        <property type="entry name" value="Myotubularin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010569">
        <property type="entry name" value="Myotubularin-like_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10807">
        <property type="entry name" value="MYOTUBULARIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10807:SF128">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-3,5-BISPHOSPHATE 3-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02893">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06602">
        <property type="entry name" value="Myotub-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00568">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51339">
        <property type="entry name" value="PPASE_MYOTUBULARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myotubularin phosphatase" evidence="12">
        <location>
            <begin position="215"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="633"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="23"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="37"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate" evidence="9">
        <location>
            <position position="427"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="365"/>
            <end position="366"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="427"/>
            <end position="433"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR630564-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR630564-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8028352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="652" mass="74960" checksum="C6E501F5746C1840" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BKT7</accession>
    <name>A0A9Q1BKT7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF4190 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_30743</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BKT7"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8028492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="91" mass="9843" checksum="D91620F1AE49B3AD" modified="2023-09-13" version="1">MKVPWDDDSEIKTIRNKAITSVALSTALSVCLYPLFCSLPAFILGILALCTLRKDTSSAKVYANVSLGLMIAAAIFITVLFLAYVIIGLIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BKT9</accession>
    <name>A0A9Q1BKT9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">cysteine--tRNA ligase</fullName>
            <ecNumber evidence="3">6.1.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Cysteinyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">HOLleu_30708</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="22">Nanhai2018</strain>
            <tissue evidence="22">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">In addition to its role as an aminoacyl-tRNA synthetase, has also cysteine persulfide synthase activity. Produces reactive persulfide species such as cysteine persulfide (CysSSH) from substrate cysteine and mediate direct incorporation of CysSSH into proteins during translations, resulting in protein persulfides and polysulfides. CysSSHs behave as potent antioxidants and cellular protectants.</text>
    </comment>
    <comment type="function">
        <text evidence="12">Mitochondrial cysteine-specific aminoacyl-tRNA synthetase that catalyzes the ATP-dependent ligation of cysteine to tRNA(Cys).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>2 L-cysteine = S-sulfanyl-L-cysteine + L-alanine</text>
            <dbReference type="Rhea" id="RHEA:78543"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:58591"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:78544"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>S-disulfanyl-L-cysteine + tRNA(Cys) + ATP = (S)-disulfanyl-L-cysteinyl-tRNA(Cys) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:78651"/>
            <dbReference type="Rhea" id="RHEA-COMP:9661"/>
            <dbReference type="Rhea" id="RHEA-COMP:19120"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:229465"/>
            <dbReference type="ChEBI" id="CHEBI:229521"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:78652"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>S-sulfanyl-L-cysteine + L-cysteine = S-disulfanyl-L-cysteine + L-alanine</text>
            <dbReference type="Rhea" id="RHEA:78627"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:58591"/>
            <dbReference type="ChEBI" id="CHEBI:229465"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:78628"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>S-sulfanyl-L-cysteine + tRNA(Cys) + ATP = (S)-sulfanyl-L-cysteinyl-tRNA(Cys) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:78647"/>
            <dbReference type="Rhea" id="RHEA-COMP:9661"/>
            <dbReference type="Rhea" id="RHEA-COMP:19119"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58591"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:229520"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:78648"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>tRNA(Cys) + L-cysteine + ATP = L-cysteinyl-tRNA(Cys) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:17773"/>
            <dbReference type="Rhea" id="RHEA-COMP:9661"/>
            <dbReference type="Rhea" id="RHEA-COMP:9679"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78517"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.16"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="18">
            <dbReference type="Rhea" id="RHEA:17775"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.16" evidence="3"/>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BKT9"/>
    <dbReference type="OrthoDB" id="438179at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004817">
        <property type="term" value="F:cysteine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006423">
        <property type="term" value="P:cysteinyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00672">
        <property type="entry name" value="CysRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000027">
        <property type="entry name" value="Cysteine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1910">
        <property type="entry name" value="Cysteine-tRNA ligase, C-terminal anti-codon recognition domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00041">
        <property type="entry name" value="Cys_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015803">
        <property type="entry name" value="Cys-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015273">
        <property type="entry name" value="Cys-tRNA-synt_Ia_DALR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024909">
        <property type="entry name" value="Cys-tRNA/MSH_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032678">
        <property type="entry name" value="tRNA-synt_1_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00435">
        <property type="entry name" value="cysS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10890:SF27">
        <property type="entry name" value="CYSTEINE--TRNA LIGASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10890">
        <property type="entry name" value="CYSTEINYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09190">
        <property type="entry name" value="DALR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01406">
        <property type="entry name" value="tRNA-synt_1e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00983">
        <property type="entry name" value="TRNASYNTHCYS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="19" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 22" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="tRNA synthetases class I catalytic" evidence="20">
        <location>
            <begin position="69"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="Cysteinyl-tRNA synthetase class Ia DALR" evidence="21">
        <location>
            <begin position="408"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="507"/>
            <end position="541"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00043868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF01406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF09190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KAJ8028472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="566" mass="64008" checksum="21684EBCEEFC1466" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BMR2</accession>
    <name>A0A9Q1BMR2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">RNA polymerase II elongation factor ELL</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_28662</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the ELL/occludin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BMR2"/>
    <dbReference type="OrthoDB" id="6284217at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008023">
        <property type="term" value="C:transcription elongation factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000987">
        <property type="term" value="F:cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042795">
        <property type="term" value="P:snRNA transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2670">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042065">
        <property type="entry name" value="E3_ELL-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031176">
        <property type="entry name" value="ELL/occludin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019464">
        <property type="entry name" value="ELL_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010844">
        <property type="entry name" value="Occludin_ELL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23288">
        <property type="entry name" value="OCCLUDIN AND RNA POLYMERASE II ELONGATION FACTOR ELL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23288:SF17">
        <property type="entry name" value="RNA POLYMERASE II ELONGATION FACTOR ELL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10390">
        <property type="entry name" value="ELL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07303">
        <property type="entry name" value="Occludin_ELL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144292">
        <property type="entry name" value="occludin/ELL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51980">
        <property type="entry name" value="OCEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="OCEL" evidence="8">
        <location>
            <begin position="522"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="140"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="334"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="373"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="145"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="236"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="334"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="379"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="389"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="441"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="476"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="499"/>
            <end position="508"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8029299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="632" mass="71463" checksum="28A8255C4AFF212D" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BNZ3</accession>
    <name>A0A9Q1BNZ3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">WW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_29644</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8030068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BNZ3"/>
    <dbReference type="OrthoDB" id="6064907at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WW" evidence="2">
        <location>
            <begin position="67"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="88"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8030068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="320" mass="37035" checksum="D51F03B8C1D19B17" modified="2023-09-13" version="1">MEYFFIILAEDIAEFSICGILSLVNYIKEIITPCIWEILKRQSKFRDLVKGCIQKTMELSEELCQQGWTLQWSCTFDWPYYFNTRTGQSQWHHPGQTIRIDQEQSRKRRHSGDDDVQSRQHGGADPEASVEDDQSEQGTISPEICYTIEAPRTRRLKRFRTSTNEYRVTFNEYLLQRLVQQHKGPLSALYNVMETLMNQIMDGFAPQDRVGVELSAPSLSHAVWVPLMRRDQITTERMFGHIEKVIQSNADFKLNGDVTFNVLHVDMANGKGPTHTNLRHWLIKKKKSVITINNTDDLCLARALVTAKARLDKANDRTIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BQD1</accession>
    <name>A0A9Q1BQD1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_27154</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BQD1"/>
    <dbReference type="OrthoDB" id="6778718at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47018">
        <property type="entry name" value="CXC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47018:SF3">
        <property type="entry name" value="MYCBP-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8030680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="206" mass="23700" checksum="4B7A09C3C2BEAD42" modified="2023-09-13" version="1">MFIAATRKGDWKLYLATTEDLLPYFHAHDQYNYGRWGPLYVADMLELQTTDPELWKFFEEGNFIITKHEIPFTGIDPDHAIEQEHRKIKAKGGFVELTGNETALAKYAIIAPSLARIVQEFKEYAGIQGRQPCTLHHETVGEKSTQMIKYAADVVDVITKQGNPFMQSDMFNLVTYAVTPAKVAKTLRIETIWEEKHLRNLYHPLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BQX3</accession>
    <name>A0A9Q1BQX3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_27766</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CFAP97 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8031135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BQX3"/>
    <dbReference type="OrthoDB" id="2163395at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038792">
        <property type="entry name" value="CFAP97D1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029488">
        <property type="entry name" value="Hmw/CFAP97"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33768">
        <property type="entry name" value="MIP11318P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33768:SF3">
        <property type="entry name" value="MIP11318P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13879">
        <property type="entry name" value="Hmw_CFAP97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="189"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="196"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="214"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="241"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8031135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="253" mass="31246" checksum="1B953F79952ADB7D" modified="2023-09-13" version="1">MCIHCVQTQSFTYLATLNRGVSTGRFLPKTEFQSNYFGKSYIVFNYITIKMHKAYQSILPCQSRILKERWDRKYYDEHRDKLEEERLATIERDNRILLEKMSEIMRTKGRVDNHNDYISKSLNREKRQRELLRVTRENQEILKRILAREPEYNHLQWEKDWEENEQFMDNVSRYPRDWWELQQKEEEMRKQELKERRKKQRKADRKEKKKSKERTRSREEDSPTEKERRPSSSEEEKRQEEEEETKPEEEAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BVM5</accession>
    <name>A0A9Q1BVM5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Fibrinogen C domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_23741</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BVM5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd19941">
        <property type="entry name" value="TIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037579">
        <property type="entry name" value="FIB_ANG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040941">
        <property type="entry name" value="GGGWT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47221">
        <property type="entry name" value="FIBRINOGEN ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47221:SF6">
        <property type="entry name" value="FIBRINOGEN ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00186">
        <property type="entry name" value="FBG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040224483" evidence="7">
        <location>
            <begin position="24"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="8">
        <location>
            <begin position="40"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="8">
        <location>
            <begin position="289"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8033488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="406" mass="46208" checksum="794CB772426491F8" modified="2023-09-13" version="1">MHCCLLRVTQFVVLCWTGRILRGQQMTDSNNDQGTSYFYYQQPEHPRDCAEIQNQCSSGISSGVYLIRPDGFPDPFEIYCNNTNSEGWTVILRRDNGFVGFDRNWNEYKDGFGFLSQDFWIGHEKLSFVTNQKNYKLRIDITNSNGLSCYLNYEVFRTSDEFGNFKVTSLEQYSGNADECISFCSSNMIPGDCNCQKTCSNPSVCVNTCSTDDTCVCPNGFYLKGEECVPLSQCTSGCFVDGEIVPEGGSYVNADCSRRGVCNNGQLTWNDAYSCNSNEVCEVRNNVRQCYSDDVMDCLDIYNAGFSDSDVYTISPANWSGSPFQVYCNMTDGGGWTVFQRRVDGTVNFYRNWTSYKEGFGTPYHENWLGNDKLYHLTNQKRYTLRIDFVNLDGSPYYAKSFLPFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BWA0</accession>
    <name>A0A9Q1BWA0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_24195</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BWA0"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="122"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8033833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="273" mass="31673" checksum="344FD9AE7EFE8688" modified="2023-09-13" version="1">MDGLLQKFREAGEESNKTVTEVTQWKEMKDILTEKAYGANPPLDNASFSSTFQSAESFAFITRDLLKILAVIVLQVYSNRRDIGRAMHDFSVAVLQRLLWPFTLLCLLWLAYFFEYDTLKKILITLCELYYIYLILFKNIRERQVTEICIILTSIALRFAKFINSSDFVGRLVVCASIIWAKYNGIGHIDFRLSGLRRLLSYFIQNPLFATCIEVMFPEACQCINLMWLTAMIAWLSINELTFMSEWNQFRSRSPNVLSKLAILIIHVLIMVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BXZ1</accession>
    <name>A0A9Q1BXZ1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Integrase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_21827</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
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    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome 10"/>
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        <property type="term" value="F:nucleic acid binding"/>
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        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
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        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF8">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase zinc-binding" evidence="1">
        <location>
            <begin position="2"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF17921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8034825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="132" mass="15460" checksum="EFEF647605349FA4" modified="2023-09-13" version="1">MINRLHASHMGIAVCLRRACECIYWLGMNSQLRDFIEQCDTCTSLDSAQSKELLQPHEVKALPWNKVQTDIFHFNDKNYLITVDYFSGFWEIDYLPDLNASTVIHKLKAQFARHGIPRTLLYVRQWPSVFLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BYA7</accession>
    <name>A0A9Q1BYA7_HOLLE</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ORF">HOLleu_22008</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
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            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BYA7"/>
    <dbReference type="OrthoDB" id="10395049at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8034957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="152" mass="16812" checksum="A368C6F85D8F8F13" modified="2023-09-13" version="1">MDSEHLDFSEDLPFESEDAGSSQQKADEFRPAGITPEMSEYIKKIVSEVTKDRPEKAGVLEQLEAKRARLGTFDDEVSSIFNACFAGSETETPEDDTLLDSVVEPFLAGDKGPALLPKTAEFIGKMMTEKLSGDKLNEKVQSYKQPENCDLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-04-02" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BYP5</accession>
    <name>A0A9Q1BYP5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Jhy protein-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_21938</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10057281at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="JHY PROTEIN HOMOLOG"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR14726:SF1">
        <property type="entry name" value="JHY PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15261">
        <property type="entry name" value="JHY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
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        </location>
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        </location>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1592"/>
            <end position="1658"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1668"/>
            <end position="1695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="28"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
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        </location>
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        </location>
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        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
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        <location>
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        </location>
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        <location>
            <begin position="542"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="557"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="573"/>
            <end position="587"/>
        </location>
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        </location>
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        <location>
            <begin position="804"/>
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        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
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        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1054"/>
            <end position="1067"/>
        </location>
    </feature>
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        <location>
            <begin position="1272"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1338"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1610"/>
            <end position="1630"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8034911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1698" mass="190011" checksum="1E21DFF06BD5D813" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BYY9</accession>
    <name>A0A9Q1BYY9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="8" type="ORF">HOLleu_22501</name>
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    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
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            <taxon>Eleutherozoa</taxon>
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            <taxon>Aspidochirotida</taxon>
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    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BYY9"/>
    <dbReference type="OrthoDB" id="6051552at2759"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005533">
        <property type="entry name" value="AMOP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051495">
        <property type="entry name" value="Epithelial_Barrier/Signaling"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003886">
        <property type="entry name" value="NIDO_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13802">
        <property type="entry name" value="MUCIN 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13802:SF59">
        <property type="entry name" value="SUSHI DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03782">
        <property type="entry name" value="AMOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06119">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00723">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00539">
        <property type="entry name" value="NIDO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50856">
        <property type="entry name" value="AMOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51220">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040321307" evidence="5">
        <location>
            <begin position="23"/>
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        </location>
    </feature>
    <feature type="domain" description="NIDO" evidence="7">
        <location>
            <begin position="107"/>
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        </location>
    </feature>
    <feature type="domain" description="AMOP" evidence="6">
        <location>
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            <end position="641"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8035317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="656" mass="76262" checksum="4A7E6D4B85054EF5" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C1R8</accession>
    <name>A0A9Q1C1R8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tyr recombinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_20692</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C1R8"/>
    <dbReference type="OrthoDB" id="6082565at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="entry name" value="Intergrase catalytic core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35617:SF3">
        <property type="entry name" value="CORE-BINDING (CB) DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35617">
        <property type="entry name" value="PHAGE_INTEGRASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8036653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="234" mass="26583" checksum="7BED668069671328" modified="2023-09-13" version="1">MLAAILSAQRQQTLHKLNIEERVITKNKEVFQIKELLKTSKPGHTGLTVEFHAYPPDRRLCAYTYLLEYLRKTKICRGKEAKLFVSYRQPHGKVSVDTIARWLKTVMHRAGVDVRQFKPHSTRTAAVSKANAANVPVSDIITGWMEKRANISKILQEATTGSFANIYERINEGLAFRDSLVYSASVQTVFIYFSFLFISSTFEGQYTCVMLYTASFVLHILQGKGTTLNSSFCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C3D4</accession>
    <name>A0A9Q1C3D4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Mitotic spindle assembly checkpoint protein MAD1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_18259</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MAD1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C3D4"/>
    <dbReference type="OrthoDB" id="331602at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051315">
        <property type="term" value="P:attachment of mitotic spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007094">
        <property type="term" value="P:mitotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.457.60:FF:000002">
        <property type="entry name" value="Mitotic spindle assembly checkpoint protein MAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.457.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008672">
        <property type="entry name" value="Mad1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23168:SF0">
        <property type="entry name" value="MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23168">
        <property type="entry name" value="MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD1 MITOTIC ARREST DEFICIENT-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05557">
        <property type="entry name" value="MAD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75704">
        <property type="entry name" value="Mitotic arrest deficient-like 1, Mad1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0498">Mitosis</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="115"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="249"/>
            <end position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8037445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="596" mass="69930" checksum="092D90F1B080134E" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C3M8</accession>
    <name>A0A9Q1C3M8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_18414</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C3M8"/>
    <dbReference type="OrthoDB" id="10042460at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="164"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="197"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="263"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="296"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="361"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="418"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="475"/>
            <end position="495"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8037569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="505" mass="58651" checksum="787891EB25B986B4" modified="2023-09-13" version="1">MAGEEDKTFPSQQFCNEVSLPMLVNKILKRQNGSLTEGTLTTPGEKWPHFIWLIMKISAIFYHDKIVTDRKCLSCETDDLERLRHGGTRSNCGRNTEVSHAEECLDVTLCYEYNTIDIAEKSVRSISSHQKRHCKACETCWWDRYGRIVRYTEDSIGLKQWNHLGSWFVSMLTLATVVSLLLYIANIAIQKLFARKGYLLSFMTYTAFLCPLTVFPIMNLCSKVRSAFSGQRIGIWATTMNARYLVQRLQFLDLSKQGLPAKFFLIICLLWPIYCSSYRAIILIALKCQHDVDAQLLCLTVFITEIIWGCFMYLLYLVRTSFQIQSCLVLDFIKKYEGHAELCQSVIRRMVVDFKCFRRCVALYLAVMIPICALGASTSLTWQYTLPARFPSGRYFCGHRDAGDRVFHHTNVMIWSEVTMFFVLGPFAFGGFDVTYIWDNFRIHTIFMQDKKFKKFWNEMIRVLEDYEIHAIPPISFPWVLSIISLYMALHFDILTPAHLARESY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C465</accession>
    <name>A0A9Q1C465_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">3-mercaptopyruvate sulfurtransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_18758</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C465"/>
    <dbReference type="OrthoDB" id="270167at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004792">
        <property type="term" value="F:thiosulfate-cyanide sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01448">
        <property type="entry name" value="TST_Repeat_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01449">
        <property type="entry name" value="TST_Repeat_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.250.10:FF:000015">
        <property type="entry name" value="Sulfurtransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045078">
        <property type="entry name" value="TST/MPST-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11364:SF27">
        <property type="entry name" value="SULFURTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11364">
        <property type="entry name" value="THIOSULFATE SULFERTANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Rhodanese" evidence="3">
        <location>
            <begin position="42"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodanese" evidence="3">
        <location>
            <begin position="173"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8037839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="294" mass="33758" checksum="B735C917C344E331" modified="2023-09-13" version="1">METQSPGPLITTHSLAKLLERPTTNMTLKILDASWHNPIKNRDARAEYKQKHIAGAAFFDTNECCDKSSPYTVMLPSAKQFEEYVGQLGIDNSSHVVVYDTYQGLRMAPRAWWMFRIFGHERISVLEGGLTKWISDGYPITNKTYEITEKNFTATYNPSLKVDFEEIYATMKSGSTHHVIDSRPVDWFNGMDYPEDEIEWKSWHIPGSVNIPWYDFVDDDGCSMKSKDEIIKMFKQNNISLHTDPIKSTCRRGIYACAVALAAFVAGRKDIPVYDGSWEEFSQRAKKEEYMTDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C4C0</accession>
    <name>A0A9Q1C4C0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_18856</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C4C0"/>
    <dbReference type="OrthoDB" id="10681873at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="83"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="114"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="146"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="183"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="384"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="433"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="442"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8037914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="458" mass="49696" checksum="24E2D8BFE5B1057C" modified="2023-09-13" version="1">MGSGARHGGRRRAAHHRRARQNRRRRHRRNHRAHAHHNNGQPFQNHIIFGAVHRRRTNDTSQTSQGVNVTYYSSSNSSVSSSALAYFPMCRVLSVFLWFAGIMMMSLGGFRTPIGFIGVGCLVVGILMLILTSLFMSGSCAQHCQSNADPEQPVPANAPEGNDQAARPVRPVVAISPPDAAAFNDSAPNYPSPDPASDKPPSYEEVMAGQYPPTISATVLPPIPEDVNTEPVLSQGMTASAPTMPPYPVNYAQAQPYGYPPVQPYPPVDVQSYQRTETERTQDPTPSYPPPITSIQVDPHMAVDTSFQVHTTQSTENGDSSQISSSFVPQPESRFEEVEPVMPLVPPQPQVAPPISSSSLVETSLSSETLPVVQVDTLQTLPLEPSSQNQTSMHLSDSSTTANNSVEHSSHGNTEVITVINEVEGTANIEKTDLQSEHVNGLPQSMDASVRTESYSEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C4G6</accession>
    <name>A0A9Q1C4G6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Nucleobase-ascorbate transporter 12</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_18941</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C4G6"/>
    <dbReference type="OrthoDB" id="1641903at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006043">
        <property type="entry name" value="NCS2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11119">
        <property type="entry name" value="XANTHINE-URACIL / VITAMIN C PERMEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00860">
        <property type="entry name" value="Xan_ur_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="54"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8037985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="112" mass="12193" checksum="31C8783E72E11CE2" modified="2023-09-13" version="1">MSKVIAKKCNSSLRFVTLVIFNVFLQPFISLIGSLILLPVLISEILCFRDEIVVLSKIISASILFQGVITLLQSTFGIRLPITQGCSFSFIPPAIALMSFYGGCSDEEGKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C4L3</accession>
    <name>A0A9Q1C4L3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Arylsulfatase G</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_19032</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sulfatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8038060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C4L3"/>
    <dbReference type="OrthoDB" id="103349at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004065">
        <property type="term" value="F:arylsulfatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050738">
        <property type="entry name" value="Sulfatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000917">
        <property type="entry name" value="Sulfatase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42693">
        <property type="entry name" value="ARYLSULFATASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42693:SF42">
        <property type="entry name" value="ARYLSULFATASE G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00884">
        <property type="entry name" value="Sulfatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14707">
        <property type="entry name" value="Sulfatase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Sulfatase N-terminal" evidence="7">
        <location>
            <begin position="2"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8038060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="401" mass="43949" checksum="6CFAB9DB191AB8F9" modified="2023-09-13" version="1">MVGKWGLGITPDYHPNSRGFDFYYGLPDSNNHGCSLEAGGTTDPNVCPRSWSDLPGYNGLHCYPCGSDIASRDPAVPLFHNRIILEQPVNQTSLDFRYAEMAAKFIKHGSKPFLLYTGLTHMHAPLFHMPEFEGQSQSNSLYGDTLLEMDSTIRTIVEAIKDSGEEENTLVWVVSDNGPDEGQCQFGGSPGPFLGVWQKTEGGGGSASKGTVFEAGHRVPSLVYWPGTIKGGQVSDSLASTMDIYPTMAALANIHLPDKRVYDGVDISELLLGKSIPKRVLYHPKVYHADGKPRCYLDAVRVGDYKAIFFTEGGRVDCEGDQGIIQSHSPPLIFNLEEDPMESSPIDPDSTLFRSVLQEVNQAMADIERSIQMDSTPIEEGEFNPCSDPCCNALNVVCRCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C4N1</accession>
    <name>A0A9Q1C4N1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">enoyl-CoA hydratase</fullName>
            <ecNumber evidence="2">4.2.1.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_19068</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.17" evidence="2"/>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8038090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C4N1"/>
    <dbReference type="OrthoDB" id="2018133at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004300">
        <property type="term" value="F:enoyl-CoA hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.12.10:FF:000001">
        <property type="entry name" value="Probable enoyl-CoA hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.10">
        <property type="entry name" value="Lyase 2-enoyl-coa Hydratase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014748">
        <property type="entry name" value="Enoyl-CoA_hydra_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941:SF54">
        <property type="entry name" value="ENOYL-COA HYDRATASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941">
        <property type="entry name" value="ENOYL-COA HYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8038090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="157" mass="17453" checksum="2392226CE04B7206" modified="2023-09-13" version="1">MKLVTSHFLSPSHRLHHARAESVLQTADVLAAVLRDSQCAGGTQRLPKAIGKSKAMEMVLTGDRLSAQDAQTAGLVSKVYPKEEVMDKAIELAEKISRNSRLINTMAKEAVNKSFEHSLSEGLHLEKRLFHASFATHDRKEGMDAFVEKRKANFKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C4Q1</accession>
    <name>A0A9Q1C4Q1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Galactosylceramide sulfotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_19092</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the galactose-3-O-sulfotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8038109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C4Q1"/>
    <dbReference type="OrthoDB" id="514299at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001733">
        <property type="term" value="F:galactosylceramide sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009247">
        <property type="term" value="P:glycolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009729">
        <property type="entry name" value="Gal-3-0_sulfotransfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14647">
        <property type="entry name" value="GALACTOSE-3-O-SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14647:SF85">
        <property type="entry name" value="GALACTOSYLCERAMIDE SULFOTRANSFERASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06990">
        <property type="entry name" value="Gal-3-0_sulfotr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8038109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="380" mass="44333" checksum="1E9AF0BCB3C5864D" modified="2023-09-13" version="1">MKTEKSLTYVVLLCFSVVGFMYLANIDEQDDIHIFMKSWEEKSSSNDSFGFSSSHETISDIKEKCSPISNIMYVKTHKTGSTTIQSILYRYGFSRNLSFVFRRQNKKNGHIRYTAVTKDSPRKMFLPILGNGSCSFTGYNISAVHVVHNRPVMETFMNPGTKYITIIRNPASQLISAFLFFHIYKGIKGNSTEEKLFKFMNGTKWRRGHYGRNSQFYDLGLSKSHFDNDTKIKERIQQLSEQMDLVLISDYFEESLVLLKQELCWSFEDLAFTVQNNRSRQKPPISPSLLEKMRKFNKADFLLYEHFNRTLWDKIAKQGHAFWEDLRKFKQVQEEVRLRCPSSTSVQKLKQANESSTFCDQYAGGLTLFKTLFKRQTRVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C4U9</accession>
    <name>A0A9Q1C4U9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">G-protein coupled receptor 52</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_19188</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8038184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C4U9"/>
    <dbReference type="OrthoDB" id="6102451at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00637">
        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050569">
        <property type="entry name" value="TAAR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24249:SF424">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24249">
        <property type="entry name" value="HISTAMINE RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="139"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="162"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="210"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="252"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="39"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8038184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="303" mass="34254" checksum="ED25D5108BB461BF" modified="2023-09-13" version="1">MGDSFTNTGMNDTDFTRSSVTVVTETLVITIISIMILVCNIGNIIILRTIRFWHKSTVYLLLNLALSDICVAIFTITAIPSAASYSHENVSDLMCKVQAFLGGTSGTVSLVTLAYISVDRYIIIVHPLRHQSLMSKRTITAMIALSWILPSVLYVWVFWDDLLANVTIFYCYGHILMIARKHSKKIFLQRTTVNVNGITAKRSYKAAGTFIAVFGMFNLCWVPYVTLVIYRALQPLSSEGDKNKVPHAVNFILPWMAMSNSFVNIFIYFGLNAQYRRAVKGLLYCQRWVYNVNDGTVYTETQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C6V4</accession>
    <name>A0A9Q1C6V4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Blue-sensitive opsin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HOLleu_13855</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Nanhai2018</strain>
            <tissue evidence="17">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8039752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C6V4"/>
    <dbReference type="OrthoDB" id="5957871at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060170">
        <property type="term" value="C:ciliary membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00637">
        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22752">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22752:SF10">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0969">Cilium</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="26"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="67"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="109"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="146"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="192"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="327"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="363"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="16">
        <location>
            <begin position="46"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ8039752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="409" mass="45989" checksum="F0E961BBB7B71E43" modified="2023-09-13" version="1">MALCKEQREWSANYSLKEHVDYHRHLILFGIEVLFLFFVTVLGLVANVSVGVAILRVPSLRRNLNNILVINLIVMDLCSVFGSMPLSFYDLFNEGYLLCFPLLCRIHGIFASLSTFGNFSAVVLIAVFRCIIIVGGQKITMKKQHVMIMLAVGWSCALAMVLPPLIGKDSKSVYTHGTHHCSPSWKSSCGYYSVGIALTYCITIPTMLICYGLIVLTIKRSADHIAEFKRKEHMDVQLTGISQMENNQNDCRIKYNQEEMSSHQDTFQEDSFSLNPCSNAQSSDQISTVDVNPQLRQHPLKSNGGGRVKRKPSRLKLPHYQRYDKKVALSGIALILTTTVCWTPYFIVHACKAQKASHGLEVFTMWLAYLNAALDPIVYTLLNRKIHKAVVQQFRFIAASAVSACSHFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C7J7</accession>
    <name>A0A9Q1C7J7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Plancitoxin-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_17222</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNase II family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C7J7"/>
    <dbReference type="OrthoDB" id="10261598at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004531">
        <property type="term" value="F:deoxyribonuclease II activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006309">
        <property type="term" value="P:apoptotic DNA fragmentation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09120">
        <property type="entry name" value="PLDc_DNaseII_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09121">
        <property type="entry name" value="PLDc_DNaseII_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004947">
        <property type="entry name" value="DNase_II"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10858">
        <property type="entry name" value="DEOXYRIBONUCLEASE II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10858:SF23">
        <property type="entry name" value="DEOXYRIBONUCLEASE II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03265">
        <property type="entry name" value="DNase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8039484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="354" mass="40365" checksum="7CE2207D754FEC0E" modified="2023-09-13" version="1">MSRATIVTSLFVLTVVSEVVYIESVTCRDMNGMTVDWFYVYKLPKITTSGNVLIKTGVAFYYLDYNRQTFQLSTVSIENLSQPVAQTLQQIYTSPAGTVDIYVAHIMYNDQPPSGRRTSRGHTKGVVSYDGKDGFWMVHSVPKFPGKSSPYQWPGNANTYGQSILCVSFKSPEMENIANQMLYNNPYVYSSNIPSKLKSIAPTFQKILKGEHVKSSPWFRKTRLQSRGGEDFTHYAKYRSFGKDLYVDLVAKDLSSTFYVETWRRRNVLPSDCTHRYHVYNILDIKFSPTVQFSYTKDHSKWAISESADQVICIGDINREKSQFKRGGGTLCANLPQVWNQYYGLMLCYEACVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CAK2</accession>
    <name>A0A9Q1CAK2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 9">Adenosylhomocysteinase</fullName>
            <ecNumber evidence="6 9">3.13.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_12236</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine</text>
            <dbReference type="Rhea" id="RHEA:21708"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16335"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
            <dbReference type="EC" id="3.13.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8 9">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
        <text evidence="8 9">Binds 1 NAD(+) per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Amino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the adenosylhomocysteinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.13.2.1" evidence="6 9"/>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CAK2"/>
    <dbReference type="OrthoDB" id="10007170at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004013">
        <property type="term" value="F:adenosylhomocysteinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033353">
        <property type="term" value="P:S-adenosylmethionine cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00401">
        <property type="entry name" value="SAHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1480:FF:000004">
        <property type="entry name" value="Adenosylhomocysteinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000004">
        <property type="entry name" value="Adenosylhomocysteinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1480">
        <property type="entry name" value="Adenosylhomocysteinase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00563">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042172">
        <property type="entry name" value="Adenosylhomocyst_ase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000043">
        <property type="entry name" value="Adenosylhomocysteinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015878">
        <property type="entry name" value="Ado_hCys_hydrolase_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020082">
        <property type="entry name" value="S-Ado-L-homoCys_hydrolase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00936">
        <property type="entry name" value="ahcY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004005">
        <property type="entry name" value="PRK05476.2-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23420">
        <property type="entry name" value="ADENOSYLHOMOCYSTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23420:SF0">
        <property type="entry name" value="ADENOSYLHOMOCYSTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05221">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00670">
        <property type="entry name" value="AdoHcyase_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001109">
        <property type="entry name" value="Ad_hcy_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00996">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00997">
        <property type="entry name" value="AdoHcyase_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00738">
        <property type="entry name" value="ADOHCYASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00739">
        <property type="entry name" value="ADOHCYASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 8 9" id="KW-0520">NAD</keyword>
    <keyword evidence="3 9" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S-adenosyl-L-homocysteine hydrolase NAD binding" evidence="11">
        <location>
            <begin position="193"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="159"/>
            <end position="161"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="224"/>
            <end position="229"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="250"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="301"/>
            <end position="303"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="348"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="355"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001109-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001109-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8041425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="434" mass="47915" checksum="D71DFA77D748A0B1" modified="2023-09-13" version="1">MAATKPKYKVADISLAEFGRKEIELAEFEMPGLMMMRKKYGPTKPLTGARIAGCLHMTIQTAVLIETLQELGAEVQWSSCNIFSTQDHAAAAMAKKGVPVYAWKGETDEEYLWCIEQTVIFPDGKPLNMILDDGGDLTNLIHTKHHDLLDGIYGVSEETTTGVHNLYKMIKTGDLKMPAINVNDSVTKSKFDNLYGCRESLVDGIKRATDIMLAGKVCVVAGYGDVGKGSAQSLRGFGARVIITEIDPINALQAAMEGFEVTTMDEASKKGQIFVSATGCDDIICEQHFMSMPDNAIVCNIGHFDTEIDMKWLQANAVEKVNIKPQVDRYKLSNGNHIIILAEGRLVNLGCATGHPSFVMSNSFTNQVLAQIELWTKKDEYKVGVHMLPKKLDEEVAAAHLEHLGVKLTKLTEKQVKYLGLPAEGPFKPEHYRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CE35</accession>
    <name>A0A9Q1CE35_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_10266</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CE35"/>
    <dbReference type="OrthoDB" id="10060618at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8043255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="222" mass="25723" checksum="3F4EBABB9BCD267B" modified="2023-09-13" version="1">MQQAILAVLYHSVKGQQDTERHRYCPDGTTESPSWCKFKREGSMDDKPHHLDPVFLNILRPHFERLSSPDLLERCLGGFTQNQNESFNALIWKRCPKHTYNGPHRVQTAMNLAVIQWNSGSETGRRLIFDKLGLSYGMYTREASLKKDNRRVLQSLHRETEQQKRKRVAIAQRNAQLETQKRQEEGKTYGRALFSGLTVDFDEWDSDDDMPLTALQAKNSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CE90</accession>
    <name>A0A9Q1CE90_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_10575</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CE90"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8043477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="88" mass="9688" checksum="A643E87262048416" modified="2023-09-13" version="1">MHYGKRKNPIIFGGGERSSGVTGGQILKTLLTQYLEMRYGKRKNPIIFGGGERSSGVTGGQTLKTLLTRYLKKGSSDSSQTWFIDAPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CEC4</accession>
    <name>A0A9Q1CEC4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_10463</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CEC4"/>
    <dbReference type="OrthoDB" id="6782329at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47510:SF3">
        <property type="entry name" value="ENDO_EXONUCLEASE_PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47510">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="1">
        <location>
            <begin position="29"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8043401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="501" mass="57514" checksum="4EA14B6C70FE3563" modified="2023-09-13" version="1">MLSNEGFNVFHMNSRSLNKNFNSIVDYLSIIKHNFAVIAFSETWLNNNVSPLININNYSLIEHHRTHRKGGGICLFVRRDFTYSERVDLSFFNDNVESLFIEMTAPYCNVNVIVGVIYRPPNGDISQFNDYLYDTLYKISSENKYCYILGDFNIDTLSEDSSNFLHTLYTCGFSPVITKPTRVSNSSATLIDNILSNVSFIDDTSHELRAGVLVTDISDHFPIFLNTSFKCKNSVKRRDVYIQNFSEDNIRMFVLRISAINWEHVLSKNDTNTAFDTFYDMFRDIHDACFPMIRIKGKGKGEKSSPWITSGIISSIKKKNRLYKQFLRNPSVANKLIYTRFRNKLNHVIRIAKKRYYFDKFQENRNDMKGTWKIVNELLNKNNNVNLPDTFKIGDSTVSDKQEIANSFNDYFVNLGPSLAAKIHSATLPSVYLSDPNLSSLYLFPATVDEVEKITTDSLDPRKAAGVDNLRPNIVKKGIPYILRPLCHIFNLSLSTGTFSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CFS1</accession>
    <name>A0A9Q1CFS1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Zinc finger CCCH domain-containing protein 14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_07296</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ZC3H14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CFS1"/>
    <dbReference type="OrthoDB" id="5589010at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008143">
        <property type="term" value="F:poly(A) binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043488">
        <property type="term" value="P:regulation of mRNA stability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.30:FF:000001">
        <property type="entry name" value="Zinc finger CCCH domain-containing protein 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.30">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1390.10">
        <property type="entry name" value="PWI domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040366">
        <property type="entry name" value="Nab2/ZC3H14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14738">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14738:SF29">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14608">
        <property type="entry name" value="zf-CCCH_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 9" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="11">
        <location>
            <begin position="694"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="9">
        <location>
            <begin position="694"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="77"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="289"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="425"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="511"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="90"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="116"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="158"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="192"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="211"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="250"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="437"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="523"/>
            <end position="534"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8044522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="865" mass="96574" checksum="B5931A4DB0DBA0B5" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CHT0</accession>
    <name>A0A9Q1CHT0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">D-aminoacyl-tRNA deacylase</fullName>
            <ecNumber evidence="3 7">3.1.1.96</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_08676</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a D-aminoacyl-tRNA + H2O = a tRNA + a D-alpha-amino acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13953"/>
            <dbReference type="Rhea" id="RHEA-COMP:10123"/>
            <dbReference type="Rhea" id="RHEA-COMP:10124"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:59871"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:79333"/>
            <dbReference type="EC" id="3.1.1.96"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>glycyl-tRNA(Ala) + H2O = tRNA(Ala) + glycine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53744"/>
            <dbReference type="Rhea" id="RHEA-COMP:9657"/>
            <dbReference type="Rhea" id="RHEA-COMP:13640"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78522"/>
            <dbReference type="EC" id="3.1.1.96"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the DTD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.96" evidence="3 7"/>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CHT0"/>
    <dbReference type="OrthoDB" id="275783at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051500">
        <property type="term" value="F:D-tyrosyl-tRNA(Tyr) deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00563">
        <property type="entry name" value="Dtyr_deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.80.10:FF:000001">
        <property type="entry name" value="D-aminoacyl-tRNA deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.80.10">
        <property type="entry name" value="D-tyrosyl-tRNA(Tyr) deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1160.11">
        <property type="entry name" value="Paired amphipathic helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00518">
        <property type="entry name" value="Deacylase_Dtd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003732">
        <property type="entry name" value="Daa-tRNA_deacyls_DTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023509">
        <property type="entry name" value="DTD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003822">
        <property type="entry name" value="PAH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036600">
        <property type="entry name" value="PAH_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00256">
        <property type="entry name" value="D-aminoacyl-tRNA deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10472:SF5">
        <property type="entry name" value="D-AMINOACYL-TRNA DEACYLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10472">
        <property type="entry name" value="D-TYROSYL-TRNA TYR DEACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02671">
        <property type="entry name" value="PAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02580">
        <property type="entry name" value="Tyr_Deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69500">
        <property type="entry name" value="DTD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47762">
        <property type="entry name" value="PAH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0820">tRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8045632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="318" mass="35689" checksum="0BB3118CC975D355" modified="2023-09-13" version="1">MRVIVQRVTKASVMVGDELISSIGRGLCVLVGICKDDTQKEREYLARKVQNLRVFDDESGKRWCKSVKDKNFEILCVSQFTLYAVMKGNKPDFHNAMPGDASKQYYEEFLSLLKKGYNAEKVKDGKFAAYMQVHIQNDGPVTIQLESIPDIHFQIPENRVGPRAASGSKTPVAVVRGQPQDRTIEKASGYIAIFTSAFNGDDTKQKQFLKILQDYSEGSIHHIPELILSAVDILHGEKELLCGFSGFLPQGFSIFLENNDLQQVRIRCPAVEDMSVEELKQHVEELKSNEASASQSSQNKVTRAMEELILQLSRLLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CHZ3</accession>
    <name>A0A9Q1CHZ3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_08254</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CHZ3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8045276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="75" mass="8147" checksum="2FDD6EE0EA2F7A02" modified="2023-09-13" version="1">MTVKSLTDCNKMVHADSLCQKATTPSDTTSPWSRQAFCSTHCYSSSRGGGSHRDYYAGPANTYTVTEAHINNISV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CJU2</accession>
    <name>A0A9Q1CJU2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_09177</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8046025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CJU2"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF15">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 10" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Helicase C-terminal" evidence="9">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="65"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="69"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="88"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="124"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="147"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8046025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="163" mass="18278" checksum="BC7115377897DB03" modified="2023-09-13" version="1">MTFPTISDIAYIHRIGRTGRAGRSGRAVTLFTEDDAVCLRSIANVMKNAGCDIPDYMLKLKKMTKKEKAKLEKKPIEREPIRTGPYRGKNEKLSSKSAEPDQENKKEQSKTGLSQQAKPGTKNKSSKKFKMRKQKRPGPLKGGRMGGKPKMRGKKKMKKKKGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CL24</accession>
    <name>A0A9Q1CL24_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_05409</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CL24"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8046655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="88" mass="9839" checksum="8FDEC0BA6B1EEE22" modified="2023-09-13" version="1">MRYGKRKNPFTFGGGQRSSGVTGGQTLKSLLRRYLKKMCYGERKNPIIFVGGQRSSGVTGGQTLKTLLTRYLEVGSLDKFHTLYFDAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CL40</accession>
    <name>A0A9Q1CL40_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Calx-beta domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_05235</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CL40"/>
    <dbReference type="OrthoDB" id="5916841at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044291">
        <property type="term" value="C:cell-cell contact zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000768">
        <property type="term" value="P:syncytium formation by plasma membrane fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.98.60">
        <property type="entry name" value="Cell-cell fusogen EFF/AFF, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029213">
        <property type="entry name" value="Fusogen_EFF/AFF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37415:SF1">
        <property type="entry name" value="CELL FUSION PROTEIN AFF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37415">
        <property type="entry name" value="EFF-1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14884">
        <property type="entry name" value="EFF-AFF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040240979" description="Calx-beta domain-containing protein" evidence="2">
        <location>
            <begin position="32"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="515"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="515"/>
            <end position="531"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8046539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="566" mass="62154" checksum="C5C72EF7E3CBDD23" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CL55</accession>
    <name>A0A9Q1CL55_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Fibrinogen-like protein A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_05465</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CL55"/>
    <dbReference type="OrthoDB" id="9990035at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00087">
        <property type="entry name" value="FReD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037579">
        <property type="entry name" value="FIB_ANG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47221">
        <property type="entry name" value="FIBRINOGEN ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47221:SF6">
        <property type="entry name" value="FIBRINOGEN ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00186">
        <property type="entry name" value="FBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040227366" evidence="7">
        <location>
            <begin position="21"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="8">
        <location>
            <begin position="53"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8046700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="279" mass="32140" checksum="E54B915EDA290F75" modified="2023-09-13" version="1">MEFVYLLVMILSLKQSQVSSSDSKQFQLREVLLDPKQVDHDKGRIISDACLPMRPPPHPRDCSELQSSCPGQSLVSGEYTIQPDHYPSPFLVYCDFDSEGEAWTVIQRRMDGSVNFQRNWHDYREGFGFLKTEFWLGNEKLSFLTAQNYYELRIELHNQLHEPYYAAYNSFRVGDESMKYRLILGSHYTGNATNSLRLHNMHFFTTFDADHDVKPDRNCASETSHGGGWWFHQCDECNLNGAYGVQSDFNPGGIEWNNLPGGGWGLTRTEMKIRRVVSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CMX6</accession>
    <name>A0A9Q1CMX6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Integrase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_00422</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CMX6"/>
    <dbReference type="OrthoDB" id="10066026at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331:SF5">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8048206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="237" mass="27377" checksum="D7AAF4582AA2F440" modified="2023-09-13" version="1">MVLLSKAKSFDCLEADRLKSFSTWFKVKRAIALCLLWKEILRARVTKNPLPVMDISIERLSEAEVEISKMVKRHHFEEEFESLKAIKSGDKFKPMKKGSSFHKLDPFLDQKGIIRVGGRLGRFSIDRNVKHPVIVPKKCHVAELLVRHFHESVAHQGRGITSNEIRANGYWIIGCSVLVGKVINKCDTCRKLRNVSSQPKMSDLSTDRVETCSPFTYHKERAQRVKEIWCFVYMYVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CNL0</accession>
    <name>A0A9Q1CNL0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">THAP domain-containing protein 8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_00889</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CNL0"/>
    <dbReference type="OrthoDB" id="4327074at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006612">
        <property type="entry name" value="THAP_Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31751:SF42">
        <property type="entry name" value="PROTEIN CBG10204"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31751">
        <property type="entry name" value="SI:CH211-108C17.2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05485">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00692">
        <property type="entry name" value="DM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00980">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50950">
        <property type="entry name" value="ZF_THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="THAP-type" evidence="7">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="96"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="187"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="324"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="99"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="187"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="200"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="248"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="324"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="357"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8048536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="898" mass="101681" checksum="9ECCC23C87CF16F3" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CPH9</accession>
    <name>A0A9Q1CPH9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_01089</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CPH9"/>
    <dbReference type="OrthoDB" id="7694994at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="181"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="189"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8048681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="239" mass="26057" checksum="D9375F31A34A0162" modified="2023-09-13" version="1">MHSVCDCQQHQNAKLLVSVKPGIKDYRDLMAKMVCNLENRYCVMLSCENCPGKAALHDHLLQFFAEHEIDMDDTVGTIGLDQVATPDSTPVSSVTGESEPRFSSPNFHRFGWCPQRIQVGRGVNSGQGKIEPSTTHEATPGREATLGLEATPGHEFVSPSPPQVRREVLDLANLFDEEPNTSVKVPLPVGVSKSSSETSSPSVSDRFRQSLTSSSSTPSLSTGSLYSPSEQEIGFRKHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CPL3</accession>
    <name>A0A9Q1CPL3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_02419</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CPL3"/>
    <dbReference type="OrthoDB" id="6287939at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="29"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="192"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="147"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="54"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="201"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8049607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="427" mass="49442" checksum="E344F4C74261DD38" modified="2023-09-13" version="1">MAGGYCLICPGLMTKYCISTMDNLLPEIPSSPIFKDSSSDDEAKIQPGQKSPKKQKEPDHIRLDKDTYKKVKEHSAVLIQLQSTLISLTTEIKRLDNQLTTGTVPRHLQVQRNLPKLPGSLHCTEELRDSWNSLLLKAGKRLGQAWRQELSRQAKRQKERYQEKEERALADLDNEIQSVEAKRLLNHLLKKSSQKTETKIAHRHKKSERRHHPYRRKVTATRSQLNAHNLNEKVEESLKYVKNLSERQLSNLEIIALSKGLKFITTPEKPNRIDILKSMKDLTRKMRIRYVMQNKMRGSFTKFRLPSTWSPQVTYSRDLEDYLEATKEELAAIPISNAKSNISKAERIALRQISKDRTIVLKPFDKGRGIAILNRTDYKADLERQLKSHHYQELEGDITSDTKIMVWSTLRNICFPTRSLTRILSIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CQ15</accession>
    <name>A0A9Q1CQ15_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HOLleu_01975</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Nanhai2018</strain>
            <tissue evidence="15">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the histone deacetylase HD2 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CQ15"/>
    <dbReference type="OrthoDB" id="1933825at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002737">
        <property type="entry name" value="AGAP008430-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000557">
        <property type="entry name" value="zinc finger and SCAN domain-containing protein 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000352">
        <property type="entry name" value="zinc finger protein 3 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000110">
        <property type="entry name" value="Zinc finger protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.340">
        <property type="entry name" value="Nucleoplasmin core domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050752">
        <property type="entry name" value="C2H2-ZF_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041232">
        <property type="entry name" value="NPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24384:SF189">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24384">
        <property type="entry name" value="FINGER PUTATIVE TRANSCRIPTION FACTOR FAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17800">
        <property type="entry name" value="NPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="15"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="502"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="530"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="558"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="586"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="614"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="642"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="670"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="696"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="724"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="752"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="780"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="808"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="864"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="892"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="920"/>
            <end position="947"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="130"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="240"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="362"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="409"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="462"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="248"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="309"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="425"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ8049293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="948" mass="107738" checksum="72E860C5363F8C00" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CQS6</accession>
    <name>A0A9Q1CQS6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein phosphatase inhibitor 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_01800</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein phosphatase inhibitor 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CQS6"/>
    <dbReference type="OrthoDB" id="551302at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004864">
        <property type="term" value="F:protein phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PPI-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12398:SF20">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY INHIBITOR SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN PHOSPHATASE INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04979">
        <property type="entry name" value="IPP-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="22"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="133"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="26"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="69"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="86"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="135"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="150"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8049178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="164" mass="19171" checksum="C3CD35A380E34BA7" modified="2023-09-13" version="1">MAPKTSTFIDGIKWDEMNILATYHPPGKDYGHMKIDEPDTPYNKYHDPERDDDDPHSDTEGVAELSPDDVSKRLEKDTLQKRWWEQDEVNEDSSEEENEDLTEEEIAKRKAFESKRKQHYNEAMFMKRAKQLIAEEFDEDEDEDEKNDVPASTVTEESVQIGQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CRH7</accession>
    <name>A0A9Q1CRH7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_03078</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="3263820at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46579">
        <property type="entry name" value="F5/8 TYPE C DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46579:SF1">
        <property type="entry name" value="F5_8 TYPE C DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8050043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="469" mass="53369" checksum="C23BC7D77E3250B0" modified="2023-09-13" version="1">MVQFNSFYGCGQCEQKAITVAKGNGTVRVFPFANPPARIRSDGDIAQQARQAVETGNSEKGVKGPSAVASIPFFSVAKGFVTDYMHCVLLGVVRSMLNLWVDSSYHQSPWYIGTRVRDIDQRLLSVQPPNIITRTPRSLTTRKYWKASEYRTWLLWYAIPVLQGILQTPYLQHFMLLCISIYLLVSRSVSKRNVLLAKHLLDSYVEKVQTLYGTEHCSFNVHQLIHLPLRVQRWGPLWVYSAFPFEDTNQILKQMIHSTQAAHLQVVGALAVTRKLKNFTQQVDGDEEAIHLAAKVSGKRTSRNQTRLDADCSFVDNGSKEVLEGPASEAAFRFIGTPMDHEVFVHKRAVVGSWMVHSRRYEQKGDNQQLKRNNSTVYFMDGPQLKIGKVEYFFKDGDQGYAIINRLNSTSQKFVHQESKSKMEQLTCVRQTNDLIVVKLSSIVETGIWMEIADKIIIGRLPNIYEYNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CS04</accession>
    <name>A0A9Q1CS04_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SWIM-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_02982</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CS04"/>
    <dbReference type="OrthoDB" id="6155932at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47526">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47526:SF4">
        <property type="entry name" value="SWIM-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="2">
        <location>
            <begin position="132"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8049986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="306" mass="34389" checksum="EFF2B31D4BF01754" modified="2023-09-13" version="1">MQVEHTSVTLTCSQYTSHLSSEELESYQEKINKLGISDPYTSPQILYSPIAAASVDTVPDLEYPDKVNYLIFNPSPYTGKVLKAYKSTDAYKYFQSGWVQDLFVWQLEGHNLYVIKAKVRHSQRLNDPLLHPWVAINKDGTVISAHCNCMAGLGEACSHIAATLFAVSAANSTLKNQSCTSLKCTWSQPSNESKQAAPFERGKDITFTYTSRKRKESQSKAAVSNPIKNYRLDFYKALHESEASEKVPCKSAILALIPEYSDEYIPKAVKFKIPPSLAELYETEFTTYAPTTGRERQSCYAVHEHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CSB2</accession>
    <name>A0A9Q1CSB2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_04632</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8051167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CSB2"/>
    <dbReference type="OrthoDB" id="427518at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071222">
        <property type="term" value="P:cellular response to lipopolysaccharide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051713">
        <property type="entry name" value="T-cell_Activation_Regulation"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25466:SF9">
        <property type="entry name" value="FIBRONECTIN TYPE-III DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25466">
        <property type="entry name" value="T-LYMPHOCYTE ACTIVATION ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="479"/>
            <end position="502"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8051167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="738" mass="83360" checksum="EA42F8DF71D047C4" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CSH8</accession>
    <name>A0A9Q1CSH8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_03394</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CSH8"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="99"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="141"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8050260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="154" mass="17442" checksum="30751F19CEBE6E6D" modified="2023-09-13" version="1">MPVRRRKMCLFVIRYVNAYTRLSFNWYCFEQDYWKTKLSDKLRNERKHLGGIHTNPKKLKVAESTEGGVQKMQGVSRCIRTNLPPVTPKTKPHKDHTPKSTTHPKTTPRPTATPSPKATPRPKASPRPTATLSPKATPRPKATPSPKATPRPKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CSQ3</accession>
    <name>A0A9Q1CSQ3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">One cut domain family member 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_02653</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CUT homeobox family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CSQ3"/>
    <dbReference type="OrthoDB" id="10257567at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.260.40:FF:000005">
        <property type="entry name" value="One cut domain family member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003350">
        <property type="entry name" value="CUT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051649">
        <property type="entry name" value="CUT_Homeobox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14057:SF47">
        <property type="entry name" value="HOMEOBOX PROTEIN ONECUT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14057">
        <property type="entry name" value="TRANSCRIPTION FACTOR ONECUT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02376">
        <property type="entry name" value="CUT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01109">
        <property type="entry name" value="CUT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51042">
        <property type="entry name" value="CUT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0371">Homeobox</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="CUT" evidence="9">
        <location>
            <begin position="250"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="234"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8049759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="383" mass="42210" checksum="E234E56BBF91B239" modified="2023-09-13" version="1">MLSSELATLDTGSILGETAAALQDSSETVQQPAFPGDLLTSELTSFVRHSRAGSLSVAGISSMLEPDYRSISADYGRASLGMNGFQASGGASYTTLTPLQPLPPISTVSDKFRHDPNVGSGFPFMNGINGSNLGMSSPYEPYSTMNMGVGVGGGMGTNAVLNGYGQSVGMGGYPSPHHGTCSPADSRYTPTSYMNGYDHFSAQTGSPHHHHMRSHTQRPGLANPMVINGFSSHPTSISPSTPMRDRSSNKEMVSNGEEVNTKEVASRVTSELKRYSIPQAVFAQRVLCRSQGTLSDLLRNPKPWSKLKSGRETFRRMWKWLQEPEFQRMSTLRLAHVKRKKMMLKRPHRTIMSRNHVLYSLIYNEEHFTQFSKRTRGHQRKCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CT60</accession>
    <name>A0A9Q1CT60_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Vacuolar protein sorting-associated protein 26B-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_03560</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the VPS26 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CT60"/>
    <dbReference type="OrthoDB" id="3821113at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.640:FF:000001">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 26A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.640:FF:000002">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 26A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028934">
        <property type="entry name" value="Vps26-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12233">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING 26 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03643">
        <property type="entry name" value="Vps26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8050375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="318" mass="37132" checksum="1210677979CAD110" modified="2023-09-13" version="1">MSFLGFGQGADIDIILDDAATRKQAEIKTEDGRKEKLYLFYDGESITGKVNVTLKGKKLEHQGIRIEFVGQIELYYDRGNHHEFTSLIKELARPGELTQSTSFPFEFMNVEKPYESYTGANVRLRYFLRVIITKRLTDTTREMDILVHTLSQYPETNSSIKMEVGIEDCLHIEFEYNKSKYHLKDVIVGKIYFLLVRIKIKHMELAIIKRETTGSGPNTFNENETIAKYEIMDGAPVRGESIPIRLFLSGYELTPTMRDVNKKFSVRYYLNLVLVDEEERRYFKQQEITVWRKPDRAKKPVIFQQLETRKQPETEEGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CTQ9</accession>
    <name>A0A9Q1CTQ9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_03686</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CTQ9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8050469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="66" mass="7837" checksum="A08D1E533A8B3924" modified="2023-09-13" version="1">MHSYSAKRYDIPYYFLTTSTEQGNCKNLWPMYKNIAHSYFQPDTVIVHLHYTHKNQQASFDPCTCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CTW0</accession>
    <name>A0A9Q1CTW0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Immunoglobulin subtype domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_04150</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CTW0"/>
    <dbReference type="OrthoDB" id="6353782at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040374543" description="Immunoglobulin subtype domain-containing protein" evidence="2">
        <location>
            <begin position="19"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="201"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8050810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="258" mass="28517" checksum="791EF75990EE4491" modified="2023-09-13" version="1">MNLHITIWLLTLTLISLSSFTQEAGNANPCSPPKTVSLTIGQEGIIECICDDSISLYWYFGNDTTAVKPFIYIDNSVKYGPGYSSGEYDIDVNGSLVIRNVTSIHGGTYAVLIIEPGNSVPRAEIFTVDVIGPEETDVMETYKNDQERALDNPEKGATITLDEPGKTSTSPVNDNYEQFVEVADITSLQEEIDQMKRIDRAMLALLVIVLIGIVLVIWRSYTVPLSKNSNCKEERVRCHQDLKQFLVACPGSCPCGKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H0W9</accession>
    <name>A0A9Q1H0W9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HOLleu_27519</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Nanhai2018</strain>
            <tissue evidence="16">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the DEAD box helicase family. eIF4A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H0W9"/>
    <dbReference type="OrthoDB" id="10265785at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18046">
        <property type="entry name" value="DEADc_EIF4AII_EIF4AI_DDX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000089">
        <property type="entry name" value="Eukaryotic initiation factor 4A-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000031">
        <property type="entry name" value="Eukaryotic initiation factor 4A-III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044728">
        <property type="entry name" value="EIF4A_DEADc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 12" id="KW-0347">Helicase</keyword>
    <keyword evidence="4 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 16" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="15">
        <location>
            <begin position="11"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="13">
        <location>
            <begin position="42"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="14">
        <location>
            <begin position="223"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="11">
        <location>
            <begin position="11"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ8030957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="384" mass="43729" checksum="C51D89B5C8E97557" modified="2023-09-13" version="1">MIETNYEEVFESFDDMNLTENLLRGIYAYGFEKPSAIQQRAIVPCCKGKDVIAQAQSGTGKTATFSIAVLQQIELSMSDCQALVLAPTRELAQQIQKVMIALGDYMNASCHACIGGTLVREDIKKLSNGQHVVVGTPGRVFDMMTRRALDTSKIKIFVLDEADEMLSRGFKDQIYDVFKNLSSTIQVILLSATMPPDVLEVTKRFMRDPIRILVKKEELTLEGIKQFYIQVEKEEWKLDTLCDLYETLTITQAVIFLNTRRKVDWLNGKMTERDFTVSSMHGDMDQKERDVIMKEFRTGSSRVLITTDLLARGIDVQQVSLVINYDLPTNRENYIHRIGRGGRFGRKGVAINFITDEDVRSLRDIETFYNTQIEEMPMNVADLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H333</accession>
    <name>A0A9Q1H333_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Bcl-2-related ovarian killer protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_27871</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Bcl-2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8031213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H333"/>
    <dbReference type="OrthoDB" id="5947850at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051400">
        <property type="term" value="F:BH domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097192">
        <property type="term" value="P:extrinsic apoptotic signaling pathway in absence of ligand"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008630">
        <property type="term" value="P:intrinsic apoptotic signaling pathway in response to DNA damage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001836">
        <property type="term" value="P:release of cytochrome c from mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06845">
        <property type="entry name" value="Bcl-2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.437.10">
        <property type="entry name" value="Blc2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036834">
        <property type="entry name" value="Bcl-2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046371">
        <property type="entry name" value="Bcl-2_BH1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026298">
        <property type="entry name" value="Bcl-2_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002475">
        <property type="entry name" value="Bcl2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11256">
        <property type="entry name" value="BCL-2 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11256:SF48">
        <property type="entry name" value="BCL-2-RELATED OVARIAN KILLER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00452">
        <property type="entry name" value="Bcl-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01862">
        <property type="entry name" value="BCL2FAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00337">
        <property type="entry name" value="BCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56854">
        <property type="entry name" value="Bcl-2 inhibitors of programmed cell death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50062">
        <property type="entry name" value="BCL2_FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="275"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="Bcl-2 Bcl-2 homology region 1-3" evidence="8">
        <location>
            <begin position="113"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8031213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="309" mass="36246" checksum="E3CD14D9F8F32416" modified="2023-09-13" version="1">MKKIREEPAFIKILRKLEYERHCLSRRAYIRTLVRQLRRAIIHTFFEKVKIIEYKVYRSLRLPQVQSEDEIVEMSYSLFRDYVQYRLPNSLKPNINPKFRKKAKTNNTIFYELQVIGNELDRQYSQLYKDIALQLNVSISSKRAVEEVFHDIAKEIFKDEITWVRIVSLYVVSSSLVIECLLQGNGIYMQYILNCFKEFVRTDLATWIVQHGGWSKGNPQGTLTPGYASDNSSLQKQERAKESGGHNCSKCITVTQYINWGDLVRTFHGHWLTDYPVLLMAVTSFCFIILYVIVALFESAVLTSRRQET</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H441</accession>
    <name>A0A9Q1H441_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Estradiol 17-beta-dehydrogenase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_26258</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H441"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040143314" evidence="1">
        <location>
            <begin position="24"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8032669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="122" mass="13761" checksum="73B0D478DFAE28FC" modified="2023-09-13" version="1">MLVFMKLVFLTRVTIICPGPVDTKFGPSMAMFDQNESDEETMAIRKQYLKNLQQIISGNIQTSQEVAKVIKEAIEADKPNFRYYTSEKDLKDARAKLADTTGNAAIQFINNTFFEGVKLSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H488</accession>
    <name>A0A9Q1H488_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_25799</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H488"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8032308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="68" mass="7929" checksum="D780785701233EB0" modified="2023-09-13" version="1">MESSREFNFNAAFLELSRSENCLRISPSLVIIRSWNQFLRYSTLQFYYCTIVDVHALPALGPDQNNIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H727</accession>
    <name>A0A9Q1H727_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Signal peptide peptidase-like 2B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_19302</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase A22B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8035579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H727"/>
    <dbReference type="OrthoDB" id="29661at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098554">
        <property type="term" value="C:cytoplasmic side of endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030660">
        <property type="term" value="C:Golgi-associated vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098553">
        <property type="term" value="C:lumenal side of endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042500">
        <property type="term" value="F:aspartic endopeptidase activity, intramembrane cleaving"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033619">
        <property type="term" value="P:membrane protein proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007369">
        <property type="entry name" value="Peptidase_A22B_SPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006639">
        <property type="entry name" value="Preselin/SPP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12174:SF103">
        <property type="entry name" value="INTRAMEMBRANE PROTEASE (IMPAS) FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12174">
        <property type="entry name" value="SIGNAL PEPTIDE PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04258">
        <property type="entry name" value="Peptidase_A22B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00730">
        <property type="entry name" value="PSN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040387623" evidence="8">
        <location>
            <begin position="17"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="170"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="230"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="256"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="297"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="324"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="418"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="447"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8035579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="493" mass="54446" checksum="28DB24F8555EE36A" modified="2023-09-13" version="1">MEWIKVLVFITSLVSALSADQGILLVRYGHPEHPNIEPFCIQYESTEGTLSPTYEEKDFHPLVDLTPDPLCEIPTDTSPFYNSIVVALGSPNCTVTERGAIAEEADAVGVVVVSVSNDLSYGNTTTNITEVVMSEDDHLELLNIQNQHKDSDIEAVLYAPELPPFDPNIFVIWTLAVVVVAIGGYWSGSTSFIVYKKKLAKRARRSHGEDGSDAEEDEDEEEESLTMSTAIILVWVVLIVAFLLLLFFFYNVCVYIVIFMFCLGAWNALFICLIPFVRKVVKWSARTPVIPLLKTQFYYVSIVFACICLVPVVCWFVYRHEDFAWILQDILGAAFCISVLKIIHMPNFKGESVMIKVATGGEGATEKLPILFMFPSFSSSPYRSCKGYSLLGFGDVLIPGLLVGFCHGFDLKVRSMKVYFVASVCAYGVGLLCTFLALILMETGQPALVYIVPITLVTVTVIGLLRRELKALWNGAPLKDHAPLGGAINAAAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H7Q2</accession>
    <name>A0A9Q1H7Q2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Reverse transcriptase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_22719</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09274">
        <property type="entry name" value="RNase_HI_RT_Ty3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000115">
        <property type="entry name" value="Polyprotein of retroviral origin, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000032">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 297-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon gypsy-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054465">
        <property type="entry name" value="Integrase_p58-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041373">
        <property type="entry name" value="RT_RNaseH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22938">
        <property type="entry name" value="Integrase_p58_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17917">
        <property type="entry name" value="RT_RNaseH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="9">
        <location>
            <begin position="872"/>
            <end position="1030"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase" evidence="8">
        <location>
            <begin position="1286"/>
            <end position="1463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="94"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="341"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="605"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="705"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="341"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="356"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="380"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="605"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="649"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="674"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="721"/>
            <end position="735"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8035476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1678" mass="191914" checksum="9B0F064C8DDB237E" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H8N5</accession>
    <name>A0A9Q1H8N5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_20719</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H8N5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8036675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="70" mass="7632" checksum="21C430A5B00B4917" modified="2023-09-13" version="1">MNFVWVISGHPADVVIEVTYEEAVTATTVNGFKNVIDPILRKRQGLYISQKRLSAPVFTTPSAVTAGGLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HAP1</accession>
    <name>A0A9Q1HAP1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Helix-turn-helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_13192</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8042192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HAP1"/>
    <dbReference type="OrthoDB" id="5982932at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058912">
        <property type="entry name" value="HTH_animal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21301">
        <property type="entry name" value="REVERSE TRANSCRIPTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21301:SF10">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26215">
        <property type="entry name" value="HTH_animal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helix-turn-helix" evidence="1">
        <location>
            <begin position="99"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF26215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8042192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="199" mass="23148" checksum="EB63F5D0D8721070" modified="2023-09-13" version="1">MGSYDGAECCELVVVYLLSQLKPYYGNSIGLYRDDGLAVFNEPPRTIEQIKKNICEIFKSNGLRITIEANKRIVNFLDVTLDLQCGTYKPYLKPDNTPLYVNAKSNHPPSVIRTIPRGINHRLSNISSNENEFKKSTQQYQEALKESGHNYELQYKSKEETKRKHRARKRNITWFNPPFDLRVKTRRTAVLENCNRKFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HB13</accession>
    <name>A0A9Q1HB13_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_09407</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8042612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HB13"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8042612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="117" mass="12818" checksum="3E6AA533A9C37E97" modified="2023-09-13" version="1">MAGISNLILWLVSNNFFLLNPLSTSALPWLSVSAILANLITAENFLSPVGLVIFPTLLQYTSILSYFTTWSRLWAVSLSSVAAPSSSSVFLAVQQYQSSGIRHVFQPLIRKLVNLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HBF2</accession>
    <name>A0A9Q1HBF2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_10014</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HBF2"/>
    <dbReference type="OrthoDB" id="424543at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8043077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="52" mass="6145" checksum="271D21B4E13E0E94" modified="2023-09-13" version="1">MRRMKWVGYLIRLPAESPARQALQESLPPTRRSRGRPITTWCSRVNNDLKNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HBU4</accession>
    <name>A0A9Q1HBU4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SCAN box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_10630</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HBU4"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF13">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCAN box" evidence="2">
        <location>
            <begin position="232"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="326"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="326"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="341"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="365"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8043512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="465" mass="53806" checksum="5CCC77A30E38D432" modified="2023-09-13" version="1">MPFNLDKFVASPSVEELDSLKKSEIIKVAKHYGIEFQPLMRKDEIKRYVLEYLVDESVLPSTVLETAITVPTDNTFELKRLEIEMNKEIRLKEMEREREREEREMQREKEEREERERRKEKAREHEFRLKQLELGVIKGSDPKIGLDTGGLFDVSKHVKFVPKFQEDNVEKFFNHFEKLGEQLKWPRDKWSILIQSNFTGKAQEVYSALSIEDSMDYDKVKKAILQAYELVPEAYRQKFRKYREADTQTYVEFAYQKERHFDRWCASKKVSTFDTLRQLMLVEEFKRCVNDDIKTHLEENKADNLKEVANLADQYALTHKFSKVGQTQNKGQFSRPNKKYGNSSGTTGNSGFQSGSISPTNSGKKQPDSSRQKNDQVSSNKAAFKDISCGFCHKKGHTMANCFTLAKLKETETASNALANVENSSEVVVTLRPNSNDKIKEEYLPFVSEGFISLDGNSAHPPVKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HCW2</accession>
    <name>A0A9Q1HCW2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SGNH hydrolase-type esterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_15062</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HCW2"/>
    <dbReference type="OrthoDB" id="10172666at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="CDD" id="cd00229">
        <property type="entry name" value="SGNH_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12690">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="301"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="310"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8040693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="547" mass="60503" checksum="57243117AA92EBC4" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HDW5</accession>
    <name>A0A9Q1HDW5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcriptional coactivator p15 (PC4) C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_12463</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HDW5"/>
    <dbReference type="OrthoDB" id="2505440at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.31.10">
        <property type="entry name" value="Transcriptional Coactivator Pc4, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003173">
        <property type="entry name" value="PC4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009044">
        <property type="entry name" value="ssDNA-bd_transcriptional_reg"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02229">
        <property type="entry name" value="PC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcriptional coactivator p15 (PC4) C-terminal" evidence="1">
        <location>
            <begin position="119"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8041596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="308" mass="35389" checksum="EE92B3883B3E2A96" modified="2023-09-13" version="1">MEDNQIFPEVPDDLYNAGWRVHYNMEDDKQYFVNEQTKMRSDTLPTVVKSPSTNLTLFENANIDLTESFVPSFYSEAKEFERNLATTSGQSPSEEKPFEKGAALGKPYQKLMEDSTTRFPLGGKRHVVVKQYRGVPYVNIREYFGGETKDRLIAVCNGHGEIKISDTAREMVTYIFASMMDRVAYGYLKQNCEGCDMGWPSQLDHRCLNFGSNVPVTDMYFNDLQHLVKDEWIIDAVRDYLLKSVEIDSIRAVMQQLRKSWSQSPELSVEAMENSKVNGIIKGHLEDEFIPRWMGAIDYSSVELNYTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HFQ1</accession>
    <name>A0A9Q1HFQ1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_00240</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HFQ1"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8048072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="57" mass="6274" checksum="68D66982D7AE9E54" modified="2023-09-13" version="1">MASVVKPGKISCGRKTTPPFHAGEGHQLAIVSGTFFSLEFLAGEAGRKTFVWKRWRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HH32</accession>
    <name>A0A9Q1HH32_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_02505</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HH32"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8049662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="127" mass="14107" checksum="B136B8E953488579" modified="2023-09-13" version="1">MSLTVPIGSIHAATLHNSRLAPHPCSYSISGTWANTTVAYFTPNPPKSDRPTNVYLNFTAVDDLWFGNIRGNVTRMEDKWVYSFDTSLCGSNGICPIRKGENRVYNETLPSPSTFIRPVSLPEKCLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HHS6</accession>
    <name>A0A9Q1HHS6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GIY-YIG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_03711</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HHS6"/>
    <dbReference type="OrthoDB" id="6128308at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10442">
        <property type="entry name" value="GIY-YIG_PLEs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1440.10">
        <property type="entry name" value="GIY-YIG endonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000305">
        <property type="entry name" value="GIY-YIG_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035901">
        <property type="entry name" value="GIY-YIG_endonuc_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058912">
        <property type="entry name" value="HTH_animal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21301">
        <property type="entry name" value="REVERSE TRANSCRIPTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21301:SF10">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01541">
        <property type="entry name" value="GIY-YIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26215">
        <property type="entry name" value="HTH_animal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82771">
        <property type="entry name" value="GIY-YIG endonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50164">
        <property type="entry name" value="GIY_YIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GIY-YIG" evidence="1">
        <location>
            <begin position="297"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8050487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="399" mass="46326" checksum="245776A72FF5817B" modified="2023-09-13" version="1">MGTPVAPSYANIFMGTIEQDILNLTSHILPNTWKRFIHDIHFLWTSTSRNLSLFQTLINTRHPTIKYTFNGSQDNVQFLDIHITQNNTQLETKIYSKPTDSHAYLLPTSCHPKHTFTSIPYSQALRVIRNCPNTDTTTYHLNELQTHFNNRGYDPSTVSQQINRAKQIPRNELLTCKPRKKNKRIPLVVTYSPIFANLPRKLHKCSRHLQNSPELKSAFKEPPMVAFRRPRSLRDLLVRARKPTPLRHDISSSEPPGFFPCKSRKCMLHSYIHATNKFKSTQTNQEFPVTTHINCKSSWVIYLMTCTHCGKQYVGKTETTLYTRFSSTRSEINNFNENTQPKRLPYTIHFNSAHHTIDNVRITGTEIVTKQSRAAILHRESFWINKLRTLHPHGINVDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HHW6</accession>
    <name>A0A9Q1HHW6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_03895</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HHW6"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8050622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="109" mass="12050" checksum="5903A0EEB4486E1E" modified="2023-09-13" version="1">MPFAYFVRYWGGLSFRWFFPTLFSNSVPHSWRPTKVYCQMRETLRIMSTVGDVLQSSAGTSTGPFLMTWLVRSNPTTMRQGRKCGVSSTAGHSITAGVTCCGGSHTTPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HI49</accession>
    <name>A0A9Q1HI49_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">UDP-glucuronosyltransferase 2A3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_04267</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HI49"/>
    <dbReference type="OrthoDB" id="5835829at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008194">
        <property type="term" value="F:UDP-glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000050">
        <property type="entry name" value="UDP-glucuronosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050271">
        <property type="entry name" value="UDP-glycosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043">
        <property type="entry name" value="EG:EG0003.4 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043:SF145">
        <property type="entry name" value="FI06409P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="455"/>
            <end position="474"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8050892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="493" mass="56117" checksum="CE3E49ABF80AED0D" modified="2023-09-13" version="1">MGEGSHFSSAAALGEELVSRGHHVTFLVGSEFKHLPDNPTSSLMFEFFTVNTQVPMASFYEQMTQAKFDGVDLFWNNSFVSEITEAQSKECSFVFSSNFLTNLKSRQFDLLVFDPWWSCCVALGDILKIKKVVLNPASLSPVFLRAFGLYVNPALVREHSTGFPERMNFYQRFQNVLLSYSIPVIDHKPFNDALTNSVALGFNLMTSYLDGLIDADLFISSSDPLLYPDMYESAGHISAAGLTAKKAHRLNQDLEMFMQSSNDQSVILYSLGTYLTHLPDEFVRIFRQVFSMFRHNVIWQWKGEELPSNITQNIQMMKWIPRNDLLGHNKTRLLICHGGNNGLHEAIYHAVPLLVIPLGGDQYDVAQKVVEKGIGLRLYVTSVTVDSLYSAINTLLSDTRYQTNINKLSTLFRDRPQSPRQKAAYWIEHILKYGSGHLHSASRDLNIWQKNLIDVFAFILLLTCLNIAVSIFIFKHILKVSRRLFWAEKTKVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HJ46</accession>
    <name>A0A9Q1HJ46_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_05569</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HJ46"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8046783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="95" mass="10721" checksum="99D24F535896D29A" modified="2023-09-13" version="1">MLFRFASPKAADTRLYKKMSRTSLAWIVVLPGREISFRMNSEKGRVSAPWSKSDGILFKLATLKCIRTKSFDTVDSLELVASVYNHCVHLNVVKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HJZ2</accession>
    <name>A0A9Q1HJZ2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase aspartic putative domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_00027</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8047911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HJZ2"/>
    <dbReference type="OrthoDB" id="10068969at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005312">
        <property type="entry name" value="DUF1759"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331:SF6">
        <property type="entry name" value="DOUBLECORTIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03564">
        <property type="entry name" value="DUF1759"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8047911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="875" mass="99428" checksum="82B783F0938E988E" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YA57</accession>
    <name>A0A9Q0YA57_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_42932</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000166">
        <property type="protein sequence ID" value="KAJ8018853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YA57"/>
    <dbReference type="OrthoDB" id="10547038at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040254247" description="Ig-like domain-containing protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8018853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="473" mass="53720" checksum="D00A85D883CA7780" modified="2023-09-13" version="1">MASSYAFILVCSLLVVNKGAGTKGECSPIQYLVLGTRGIIHCSFEEEFFGVLWYNTTDVLYHDAILTFREGEKSGTGYLAGEYDVFANGSLVVYNVSKWHESKFTMLFLKTTSELPVRYNFNVLVNANTFIPFPRVNECSDIGEVCLQRWNQQSETSCFIRDARLMIPLTWMVRTIHGDRNISSVLSVTNQTSFYTSKVSTTNPFAFSARLALLVCKADDPMGLLKRNQSLVLVSKQKLDPKSENVITKYFKIGSQIRLECSANQFLFLLWQVKQTSQDKEILIIYTVLKEDTFSRRYDDDYNLESNGSLAVYDVTVKHGGLYSCISGDGITDNVIMYNIVVFVLPVPPYPVIQGCNHEQYCVLDVEREGSLTCTLTGIRPRVQLDFKAFNHQSSQRIEFFDKHLLTNDNDGTFDVFLTSQYRVTETEINKVTIDCRVIGTEIDHLQLATKFDILFMSGSKQRSPHLLNNFGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YAZ5</accession>
    <name>A0A9Q0YAZ5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_44073</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000621">
        <property type="protein sequence ID" value="KAJ8018114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YAZ5"/>
    <dbReference type="OrthoDB" id="9980069at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1820">
        <property type="entry name" value="L1 transposable element, RRM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35555">
        <property type="entry name" value="ENDONUCLEASE-REVERSE TRANSCRIPTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35555:SF3">
        <property type="entry name" value="ENDONUCLEASE-REVERSE TRANSCRIPTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="81"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="21"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8018114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="636" mass="73612" checksum="13FD156DE4809C05" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YB88</accession>
    <name>A0A9Q0YB88_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Solute carrier family 22 member 6-A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_42891</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000157">
        <property type="protein sequence ID" value="KAJ8018880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YB88"/>
    <dbReference type="OrthoDB" id="2544694at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8018880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="80" mass="8615" checksum="391EA411D6906C44" modified="2023-09-13" version="1">MGLFSTSSRIGGIVAPLILILGEVWPSLPILIFGSFSIAAGCLCLLLPETKGKPLPSTARETKSLYRFVLRNSTSPLLHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YD32</accession>
    <name>A0A9Q0YD32_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Insulin receptor substrate 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Protein chico</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_40177</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit. May mediate the control of various cellular processes by insulin-like peptides. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains. Involved in control of cell proliferation, cell size, and body and organ growth throughout development. Also has a role in a signaling pathway controlling the physiological response required to endure periods of low nutrient conditions. Insulin/insulin-like growth factor (IGF) signaling pathway has a role in regulating aging and is necessary in the ovary for vitellogenic maturation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Bindings to phosphatidylinositol 3-kinase and SHP2.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YD32"/>
    <dbReference type="OrthoDB" id="946068at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005158">
        <property type="term" value="F:insulin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043548">
        <property type="term" value="F:phosphatidylinositol 3-kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008286">
        <property type="term" value="P:insulin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048477">
        <property type="term" value="P:oogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01257">
        <property type="entry name" value="PH_IRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01204">
        <property type="entry name" value="PTB_IRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039011">
        <property type="entry name" value="IRS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002404">
        <property type="entry name" value="IRS_PTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10614">
        <property type="entry name" value="INSULIN RECEPTOR SUBSTRATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10614:SF13">
        <property type="entry name" value="INSULIN RECEPTOR SUBSTRATE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02174">
        <property type="entry name" value="IRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00628">
        <property type="entry name" value="INSULINRSI"/>
    </dbReference>
    <dbReference type="SMART" id="SM01244">
        <property type="entry name" value="IRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00310">
        <property type="entry name" value="PTBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51064">
        <property type="entry name" value="IRS_PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0221">Differentiation</keyword>
    <keyword evidence="4" id="KW-0341">Growth regulation</keyword>
    <keyword evidence="7" id="KW-0896">Oogenesis</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PH" evidence="11">
        <location>
            <begin position="17"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="IRS-type PTB" evidence="12">
        <location>
            <begin position="129"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="227"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="331"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="389"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="454"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="671"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="716"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="898"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="257"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="286"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="497"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="507"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="533"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="603"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="725"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="743"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="787"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="830"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="841"/>
            <end position="858"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00046145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8020557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="956" mass="104075" checksum="BE91295497DC1AFD" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YDK7</accession>
    <name>A0A9Q0YDK7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_40599</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YDK7"/>
    <dbReference type="OrthoDB" id="775972at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon gypsy-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041373">
        <property type="entry name" value="RT_RNaseH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17917">
        <property type="entry name" value="RT_RNaseH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="10">
        <location>
            <begin position="447"/>
            <end position="576"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8020888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="576" mass="66594" checksum="E9D9D6B5F567BDBB" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YE24</accession>
    <name>A0A9Q0YE24_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transcription initiation factor TFIID subunit 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_41316</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat TAF5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8019650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YE24"/>
    <dbReference type="OrthoDB" id="10266330at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08044">
        <property type="entry name" value="TAF5_NTD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000243">
        <property type="entry name" value="Transcription initiation factor TFIID subunit 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.500">
        <property type="entry name" value="TFIID subunit TAF5, NTD2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007582">
        <property type="entry name" value="TFIID_NTD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037264">
        <property type="entry name" value="TFIID_NTD2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19879:SF1">
        <property type="entry name" value="CANNONBALL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19879">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04494">
        <property type="entry name" value="TFIID_NTD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160897">
        <property type="entry name" value="Taf5 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3 9" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="TFIID subunit TAF5 NTD2" evidence="12">
        <location>
            <begin position="78"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="333"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="406"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="448"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="490"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="532"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="574"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="257"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="24"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="55"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF04494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8019650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="666" mass="74973" checksum="79C6965465A16B54" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YF83</accession>
    <name>A0A9Q0YF83_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Sodium-coupled monocarboxylate transporter 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_39853</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YF83"/>
    <dbReference type="OrthoDB" id="6132759at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11492">
        <property type="entry name" value="SLC5sbd_NIS-SMVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="entry name" value="Sodium/glucose cotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051163">
        <property type="entry name" value="Sodium:Solute_Symporter_SSF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00813">
        <property type="entry name" value="sss"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42985:SF40">
        <property type="entry name" value="LD47995P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42985">
        <property type="entry name" value="SODIUM-COUPLED MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="10" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="80"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="123"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="154"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="188"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="232"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="272"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="332"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="379"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="406"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="435"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="512"/>
            <end position="534"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8020295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="578" mass="62731" checksum="196970E0A0EA99AD" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YFK6</accession>
    <name>A0A9Q0YFK6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Mitochondrial nucleoid factor 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Mitochondrial protein M19</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_40094</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
            <location evidence="1">Mitochondrion nucleoid</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YFK6"/>
    <dbReference type="OrthoDB" id="6266314at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042645">
        <property type="term" value="C:mitochondrial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034551">
        <property type="term" value="P:mitochondrial respiratory chain complex III assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037698">
        <property type="entry name" value="UQCC2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34260">
        <property type="entry name" value="UBIQUINOL-CYTOCHROME-C REDUCTASE COMPLEX ASSEMBLY FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34260:SF1">
        <property type="entry name" value="UBIQUINOL-CYTOCHROME-C REDUCTASE COMPLEX ASSEMBLY FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-1135">Mitochondrion nucleoid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="61"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="83"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="105"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="115"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="145"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="195"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="209"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="233"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="260"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00031206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00032983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8020490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="391" mass="42238" checksum="B859A09FDEE4B376" modified="2023-09-13" version="1">MSEARCALCSQFRPGKGWDPHSVCPACRSCTRQDPCELCMAFSPSQWKEIDSWRRARREKLKSRLEAIPDPDPSAPGTSGTQKKQKGTGKSSKKRKRREESAQGRRGGLPGAGPSGSAAAASPGTTPSAHSEGSSPAGPVDRRTPVNTGPTGSGTTVPRTDTQAPSGLSPDPEPSTSLPLDSAGSPVPSEDRTQSEVQGATGSQSLAPTATAAGTETGSGSADPGPVIQDTQTSRDRSRSREGSSHRSRRESPARSDSRRRSRDHRSRSPSGGSRRRRRSPESSDSETDSDAGYRRDRPSRSRRTSPGGVPDLSDPAWINEKDCDRQYEALQKINTDVYRKKFPRIRETSATGSTAEECSMVMATATMEELEYLDRSFFQKAKDYFTSSKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YGV1</accession>
    <name>A0A9Q0YGV1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein/nucleic acid deglycase DJ-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_38322</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YGV1"/>
    <dbReference type="OrthoDB" id="543156at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016684">
        <property type="term" value="F:oxidoreductase activity, acting on peroxide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046295">
        <property type="term" value="P:glycolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903189">
        <property type="term" value="P:glyoxal metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010646">
        <property type="term" value="P:regulation of cell communication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0023051">
        <property type="term" value="P:regulation of signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03135">
        <property type="entry name" value="GATase1_DJ-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000022">
        <property type="entry name" value="protein deglycase DJ-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006287">
        <property type="entry name" value="DJ-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002818">
        <property type="entry name" value="DJ-1/PfpI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050325">
        <property type="entry name" value="Prot/Nucl_acid_deglycase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01383">
        <property type="entry name" value="not_thiJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48094:SF12">
        <property type="entry name" value="PARKINSON DISEASE PROTEIN 7 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48094">
        <property type="entry name" value="PROTEIN/NUCLEIC ACID DEGLYCASE DJ-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01965">
        <property type="entry name" value="DJ-1_PfpI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DJ-1/PfpI" evidence="3">
        <location>
            <begin position="41"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8021195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="226" mass="24126" checksum="5C3202DD6E9018AD" modified="2023-09-13" version="1">MLFYKTLHLKFGRGLVEPLFRSAPFYVRTFACANFEPRDKMSALLILAAGAEEMETVIPTDVLRRAEINVTVAGLAGADPVTCSRSVVIKPDKSLEDAMKAGPYDVIILPGGGPGAKALSESSKVKEVLEAQVGRNGHIAAICAAPMALLTHGIGKGQKLTSHPSIKDMLVQSGHYTYSEDRVVVDGKLITSRGPGTAFEFALALVEAIKGKDVVNKIVPPMLLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YHE9</accession>
    <name>A0A9Q0YHE9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_38704</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YHE9"/>
    <dbReference type="OrthoDB" id="2160826at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8021480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="141" mass="16325" checksum="ABF0F5F6FD857B87" modified="2023-09-13" version="1">MWFSGGVSELSSSVFGRLEALGIVVNPRDRFHPYRITYDIETYLDKEDVMPSKSAKLNYIGQHKLLSISVCSNVPGYDRPQYFTSEGDDLQLVDIFVTYMTEISQAAFDREWSRFERSPEFKRVGERLRTAGKPFRRVRIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YHG7</accession>
    <name>A0A9Q0YHG7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Tachykinin-like peptides receptor 86C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_37645</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YHG7"/>
    <dbReference type="OrthoDB" id="5981855at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004995">
        <property type="term" value="F:tachykinin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001681">
        <property type="entry name" value="Neurokn_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46925">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR TKR-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46925:SF2">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR TKR-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="56"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="1"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8022678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="150" mass="16792" checksum="526C0812910D4F7D" modified="2023-09-13" version="1">MIIWIVMSNSRTRSVTNFFLMNLAVADVLKATLDMPVLYVFILTQNWTWGSVTCKVVRFVGNMTAAASILSLIAVTIDRYRAIVYPFLPRLGKRMVSFGIYMIWSMAAIWACPNLIFGQVRSGGTNSTWTCILVWPDNAGDGVYGPYDFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YI76</accession>
    <name>A0A9Q0YI76_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Fibrinogen-like protein A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_38084</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8023023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YI76"/>
    <dbReference type="OrthoDB" id="6145874at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00087">
        <property type="entry name" value="FReD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050373">
        <property type="entry name" value="Fibrinogen_C-term_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040941">
        <property type="entry name" value="GGGWT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143:SF327">
        <property type="entry name" value="FI21813P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143">
        <property type="entry name" value="FIBRINOGEN/TENASCIN/ANGIOPOEITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00186">
        <property type="entry name" value="FBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040233123" evidence="1">
        <location>
            <begin position="24"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="2">
        <location>
            <begin position="47"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8023023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="276" mass="32230" checksum="389B885C7B0401CD" modified="2023-09-13" version="1">MKFAVVLLFIFGCSLIDITSTTGHQTQEDVPRTLQEKTVDIHSCCKHHQSQYPRDCKEIYDKCRQSAPDGIYLIQPDDAPEPFKVFCNNNVDGGGWTVLQRRLDGSIDFFRNWDEYKRGFGFLGREFWLGNDKISYLTNQKDYELRIDLNNKNGNAYYAKYDLFRISDEIGNYKLVGLGQCHSGSTAGCNGLESSRNHFFSTRDRENDLTNNRHCAEYTTGAWWYDPSSTHRCGDSMLNNQYNPDGRLQSIRWWNLPENNYFIKYTEMKIRPLQRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YIC1</accession>
    <name>A0A9Q0YIC1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">methenyltetrahydrofolate cyclohydrolase</fullName>
            <ecNumber evidence="2">3.5.4.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_38163</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(6R)-5,10-methenyltetrahydrofolate + H2O = (6R)-10-formyltetrahydrofolate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57455"/>
            <dbReference type="ChEBI" id="CHEBI:195366"/>
            <dbReference type="EC" id="3.5.4.9"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.9" evidence="2"/>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8023083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YIC1"/>
    <dbReference type="OrthoDB" id="5126881at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004477">
        <property type="term" value="F:methenyltetrahydrofolate cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004487">
        <property type="term" value="F:methylenetetrahydrofolate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004488">
        <property type="term" value="F:methylenetetrahydrofolate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01080">
        <property type="entry name" value="NAD_bind_m-THF_DH_Cyclohyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10860:FF:000005">
        <property type="entry name" value="C-1-tetrahydrofolate synthase, cytoplasmic, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01576">
        <property type="entry name" value="THF_DHG_CYH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000672">
        <property type="entry name" value="THF_DH/CycHdrlase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020630">
        <property type="entry name" value="THF_DH/CycHdrlase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020631">
        <property type="entry name" value="THF_DH/CycHdrlase_NAD-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48099:SF11">
        <property type="entry name" value="BIFUNCTIONAL METHYLENETETRAHYDROFOLATE DEHYDROGENASE_CYCLOHYDROLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48099">
        <property type="entry name" value="C-1-TETRAHYDROFOLATE SYNTHASE, CYTOPLASMIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00763">
        <property type="entry name" value="THF_DHG_CYH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02882">
        <property type="entry name" value="THF_DHG_CYH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00085">
        <property type="entry name" value="THFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="3" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tetrahydrofolate dehydrogenase/cyclohydrolase catalytic" evidence="8">
        <location>
            <begin position="37"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="Tetrahydrofolate dehydrogenase/cyclohydrolase NAD(P)-binding" evidence="9">
        <location>
            <begin position="191"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00036357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8023083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="307" mass="34129" checksum="F7E4FA6415E387BC" modified="2023-09-13" version="1">MFRYLDVLQRSIRLLSRRLNKGQKFSRRSSLFGARIIDGNKFAKDVRQEVKREIQEWIKEGHTVPQLTAVLIGNDPTSSTSIKDKMEAARDVGITSDVIRIPDSTTEMELLDIINSLNETPSVNGILLQHPLPRHVDERVICDTVHPDKDVDGFHMSNIGRLCLDKPNLIPATAYAVWELLHRSGSRMGVDATVTTCHQYTPANNLSEFTKAADILVVAVGSPCLVTADMIKDGAVVIDVGINRIWDSSQKHYKIVGDVDFEDVVKKASFISPVPGGVDPMTVAVLMKNTLQAAQIQVKKHRQDNVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YLN0</accession>
    <name>A0A9Q0YLN0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Proteasomal ubiquitin receptor ADRM1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_40920</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ADRM1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8021136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YLN0"/>
    <dbReference type="OrthoDB" id="340431at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061133">
        <property type="term" value="F:endopeptidase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070628">
        <property type="term" value="F:proteasome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13314">
        <property type="entry name" value="PH_Rpn13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.2020.20:FF:000001">
        <property type="entry name" value="Proteasomal ubiquitin receptor ADRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.70:FF:000001">
        <property type="entry name" value="Proteasomal ubiquitin receptor ADRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.2020.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.70">
        <property type="entry name" value="Proteasomal ubiquitin receptor Rpn13/ADRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044867">
        <property type="entry name" value="DEUBAD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006773">
        <property type="entry name" value="Rpn13/ADRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044868">
        <property type="entry name" value="Rpn13/ADRM1_Pru"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038633">
        <property type="entry name" value="Rpn13/ADRM1_Pru_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032368">
        <property type="entry name" value="RPN13_DEUBAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038108">
        <property type="entry name" value="RPN13_DEUBAD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12225">
        <property type="entry name" value="ADHESION REGULATING MOLECULE 1 110 KDA CELL MEMBRANE GLYCOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12225:SF0">
        <property type="entry name" value="PROTEASOMAL UBIQUITIN RECEPTOR ADRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04683">
        <property type="entry name" value="Rpn13_ADRM1_Pru"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16550">
        <property type="entry name" value="RPN13_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51916">
        <property type="entry name" value="DEUBAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51917">
        <property type="entry name" value="PRU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0647">Proteasome</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pru" evidence="9">
        <location>
            <begin position="15"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="DEUBAD" evidence="8">
        <location>
            <begin position="266"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="194"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="363"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="208"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="372"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8021136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="408" mass="43237" checksum="C93D214992E40163" modified="2023-09-13" version="1">MSGALFGHSGTPGRTPSKHLVEFRAGKMYLKGTTVTPDKRKGLVYIYQADDSLIHFCWKDRTSGVVEDDLIIFPDDCEFKRIQQCTTGRVYLLKFKSNARKFFFWLQEPKTDKDEENCKKVNETLNNPPAPGSNLGGGRGGLPADLASLGAGLGDGGLQSMLGNVDQQQLMQLLGSGGLSGLGGLGLAGLPGLAPQASSPQSATPAVTTSAGTRTSTSTSNTTTPTPAPTTAQTPAPTAAQAAAASSSIQLSDLQSILRNMNIPADQAPQARVDFAQVLTSDVMAPLLENPEFQQQLLQYAPEGENLPRDPQRLRETLQSPQFQQAMNMFTAAFQSGQLGPLIGQFELGAEATEAANKGDLEAFGKALQAKEGNKDDSQGEGKSDKEEDKSGKGDGKPGDEDDSMQVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YML8</accession>
    <name>A0A9Q0YML8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 4">Neutral ceramidase</fullName>
            <ecNumber evidence="4">3.5.1.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_38012</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>an N-acylsphing-4-enine + H2O = sphing-4-enine + a fatty acid</text>
            <dbReference type="Rhea" id="RHEA:20856"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:52639"/>
            <dbReference type="ChEBI" id="CHEBI:57756"/>
            <dbReference type="EC" id="3.5.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="3">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the neutral ceramidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.23" evidence="4"/>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YML8"/>
    <dbReference type="OrthoDB" id="191371at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017040">
        <property type="term" value="F:N-acylsphingosine amidohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046514">
        <property type="term" value="P:ceramide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042759">
        <property type="term" value="P:long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046512">
        <property type="term" value="P:sphingosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006823">
        <property type="entry name" value="Ceramidase_alk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031329">
        <property type="entry name" value="NEUT/ALK_ceramidase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12670">
        <property type="entry name" value="CERAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12670:SF1">
        <property type="entry name" value="NEUTRAL CERAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04734">
        <property type="entry name" value="Ceramidase_alk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040450492" description="Neutral ceramidase" evidence="5">
        <location>
            <begin position="19"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="domain" description="Neutral/alkaline non-lysosomal ceramidase N-terminal" evidence="6">
        <location>
            <begin position="22"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="2">
        <location>
            <position position="275"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00019235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR606823-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR606823-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU366019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8022962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="413" mass="44833" checksum="2A9E1E490791BD5F" modified="2023-09-13" version="1">MKPVLCVLLAFTLAVAAAQQTYYVGIGIEDVTGPAADVNMMGYAHPGQTSRGIHTRQYSRAFIFCDDADGNNCEVFVVVDFAMGATAINLGVLKRLETLYGNRYTEKNVVICGTHTHSAPAGFLQYFVFDVTSQGFIHDSYDNFVEGIFQSIRMAHESIAPSKVYYNSGELVGANINRSPYAYEQNPLNERNDYGSDVDTTMTVLKITGTDDTDRGLISFFPVHPVSMNNTNRLLSSDNKGYASYLIEMEMNPGTFHGQGEFVAAFANTNQGDVSPNTKGAFCLDTGEECDRITSTCNGRTSLCAGVGPGKDMFESTKIIGEKQATKAKELYDDATERINGPVGFVHQHVDMTNYEVDLGGGQTGTTCKAAFGYSFAAGATDGAGFFPFTQAIVIYHGFCGLCLKPFRVIKKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YN92</accession>
    <name>A0A9Q0YN92_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_34469</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YN92"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8024536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="66" mass="7584" checksum="631144B77855D1AE" modified="2023-09-13" version="1">MGSSRRLEQHEMRLCDIQRFVCLKPVTHTRNLSVYYGNIKEITQECQQPLKTGGITLTRLTSSTKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YQ32</accession>
    <name>A0A9Q0YQ32_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase/retrotransposon-derived protein RNase H-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_34209</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8026383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YQ32"/>
    <dbReference type="OrthoDB" id="7995463at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="CDD" id="cd09274">
        <property type="entry name" value="RNase_HI_RT_Ty3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.370:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 17.6-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041577">
        <property type="entry name" value="RT_RNaseH_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF8">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17919">
        <property type="entry name" value="RT_RNaseH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase/retrotransposon-derived protein RNase H-like" evidence="1">
        <location>
            <begin position="23"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF17919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8026383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="215" mass="24819" checksum="D74D1BC0653E8C26" modified="2023-09-13" version="1">MLNLSEITGPLRELLSKSVEWHWTEVHENCFNKLKKVLCEALVLAYYDENKDLTLSVDASSKGLGATLLQEGRPIAYALKALTNAETNYSQIEKELLAIVWGYKQFHDYVAFRKVLVESDHRPLESIMKKPLYGALLRLQRMLMQLRHYDVDVRYKKGLELYMADALSRAYLPYFGMPIECPLEVSVVKELTPISSEKFEEFKRETKRSTSCSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YQC1</accession>
    <name>A0A9Q0YQC1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Solute carrier family 35 member G1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_31620</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YQC1"/>
    <dbReference type="OrthoDB" id="5968806at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22911">
        <property type="entry name" value="ACYL-MALONYL CONDENSING ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22911:SF6">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER G1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="43"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="72"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="101"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="127"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="164"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="194"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="226"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="254"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="281"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="6">
        <location>
            <begin position="12"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8026708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="340" mass="37000" checksum="CC421621A8875D79" modified="2023-09-13" version="1">MKELREFFYTRRGIFYTIISGIGYGSLGIFAQLTPSVHTLESSTVRGLFLAFLSLIVIFASRSKPICSKNEFLWILASGSLSTGVQITLFMAYSLAPAGDVNAIVYSSPLFSGILARIFLKEPLACYDALLCTVSIAGVFLISKPEFVFGTADDLGETSNSFKGSLWAIGCLILYPIQPVIARKLRQDSTQASILTFTFGILALSTSAILNSFIGGWTIPLDAKEMFFLIGVGISGFVGAHFLNVALETEKAIFATLSVTVLTIFITYVLQFLIFHVYPDVISGTGIILIGGATIVALVVKWREAKETVVQQNNDPDTKKKDATTQTDLTFDNPAVDYQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YR94</accession>
    <name>A0A9Q0YR94_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cleavage stimulation factor subunit 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_32160</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YR94"/>
    <dbReference type="OrthoDB" id="272703at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.70:FF:000001">
        <property type="entry name" value="Cleavage stimulation factor subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.630:FF:000001">
        <property type="entry name" value="Cleavage stimulation factor subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.70">
        <property type="entry name" value="Transcription termination and cleavage factor, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025742">
        <property type="entry name" value="CSTF2_hinge"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026896">
        <property type="entry name" value="CSTF_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038192">
        <property type="entry name" value="CSTF_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45735">
        <property type="entry name" value="CLEAVAGE STIMULATION FACTOR SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45735:SF2">
        <property type="entry name" value="CLEAVAGE STIMULATION FACTOR SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14327">
        <property type="entry name" value="CSTF2_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14304">
        <property type="entry name" value="CSTF_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Cleavage stimulation factor subunit 2 hinge" evidence="6">
        <location>
            <begin position="40"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription termination and cleavage factor C-terminal" evidence="5">
        <location>
            <begin position="392"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="123"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="226"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="131"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="145"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="240"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="322"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="367"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF14304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF14327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8027112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="437" mass="46685" checksum="3693EE0AAA7A9EC4" modified="2023-09-13" version="1">MRNLNGYDINGRTLRVDYATTEKNKEEMKALQTSQAPTESPYGLPVEPHEAPEAISKAVASLPPEQMFELMKQMKIVIQNNPVEARNMLLQNPQLAYALLQAQVVMRIVSPEVAMTLLHKQNDAPQPIMPPEQQQAGPSQNQPQPVGPPQGQPQPIVPPQNQHPPMGHPQPNHGSRGPNLPGMQGPEPGMAPQRMAGTGLPVGMQGRSATQQAGMPVMMQQQASLNEPRYQDGRGPPGGPGLMGQGPRQGPGGHGMMMMQHHGEQGVGRGGIPMDQGGPMDQDPIYKDKGRRGGSQNPMGMPPRQGAVDPRGGMDPRGPPIEGHDPRRQGYDRHPEGLSHGQMPPGLGNLPQRLQGADPRGLTRGLPMGAPGGAAPAPNQGGPGSQAQPTPQDQEKAALIMQVLGLSNEQINSLPSDQRQSILLLKEQLARSQGQIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YRH9</accession>
    <name>A0A9Q0YRH9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ATP-dependent RNA helicase DHX34</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_34133</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8026321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YRH9"/>
    <dbReference type="OrthoDB" id="10253254at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17979">
        <property type="entry name" value="DEXHc_DHX34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1080:FF:000005">
        <property type="entry name" value="ATP-dependent helicase HrpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000540">
        <property type="entry name" value="probable ATP-dependent RNA helicase DHX34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000725">
        <property type="entry name" value="probable ATP-dependent RNA helicase DHX34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056382">
        <property type="entry name" value="DHX34_Znf-C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF221">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DHX34-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24485">
        <property type="entry name" value="zf-C2H2_DHX34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 8" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="6">
        <location>
            <begin position="309"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="7">
        <location>
            <begin position="503"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="33"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8026321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1279" mass="147093" checksum="72F0CCB1D60A5D7B" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YSE2</accession>
    <name>A0A9Q0YSE2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_31630</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YSE2"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8026715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="103" mass="10779" checksum="86709FBAE6DF31B4" modified="2023-09-13" version="1">MGNERAARNGKLDVGLEKQSDQGMAWGMEEMVIVKQYKLETREGITIEETFAVVSFVGVVVSSVVAVASFVDVVVSSVVAVASFVGVVVSSVASVDVALRIGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YUB8</accession>
    <name>A0A9Q0YUB8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein TFG</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_31709</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YUB8"/>
    <dbReference type="OrthoDB" id="1594986at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048208">
        <property type="term" value="P:COPII vesicle coating"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06401">
        <property type="entry name" value="PB1_TFG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000045">
        <property type="entry name" value="TFG isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053793">
        <property type="entry name" value="PB1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000270">
        <property type="entry name" value="PB1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034857">
        <property type="entry name" value="PB1_TFG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033512">
        <property type="entry name" value="TFG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15335">
        <property type="entry name" value="PROTEIN TFG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15335:SF7">
        <property type="entry name" value="PROTEIN TFG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00564">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00666">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51745">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PB1" evidence="2">
        <location>
            <begin position="23"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="150"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="196"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="225"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="247"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="305"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8026776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="374" mass="41007" checksum="8F498CEE8D62809B" modified="2023-09-13" version="1">MDGTPGMQQTDRSHFPQIDLSNKLIIKAQLGDDIRRIPIHNEDITYDELVLMMQRVFRGKLNSNDEVVIKYKDEDGDLITIFDSSDLSFAIQCSRILKLTLFVNGQPQPLESDQVKHLRRELQAIRNQVNSLLDNLEPPQIQQTDVVPVENVTSSQEPKHDGPQVAMFDPMKPQEDSSVMSSFSGHVAEEPERAASPSDSISSHGSSKHQQRAPAQYASPHHQPQDPSQQQSQVSSSQPSALPPSAPSQQQAAYGAGQPPQPPYGYQQPGIPQAQQSQQGVPQQQQQQQQYPPSSNYGGYGAYNQASQAGTYGQQQQPAPQQGYGGYAQQPQQQQYGAQPAPAGMPGQAGNPYARNTPYGRPGVAYQQPGPGYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1B988</accession>
    <name>A0A9Q1B988_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NACHT, LRR and PYD domains-containing protein 14</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_42705</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000122">
        <property type="protein sequence ID" value="KAJ8018981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1B988"/>
    <dbReference type="OrthoDB" id="427518at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312">
        <property type="entry name" value="NACHT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312:SF2">
        <property type="entry name" value="NUCLEOTIDE-BINDING OLIGOMERIZATION DOMAIN-CONTAINING PROTEIN 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NACHT" evidence="1">
        <location>
            <begin position="4"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8018981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="476" mass="55573" checksum="2E22E8AD86F28D61" modified="2023-09-13" version="1">MRRIIEGEPGYGKSTLTLQLSYDWCNKIQLSYLKDIDVLILLRLRQLGGVTSVYRAIKQFLLPRDSKLTESDIADIISECSSLLVILDGYDEYPQQDVHVDSDVISIIMRNMFQQFEVILTTRLSYLPKKYPAMTKRLKLTGFDDNARDQYIKKAVVGDDVTAAEKIIQRLKENPVLGDLCQVPLFFVMFAHMSHESKQFQKFNSVTSFFRYMISCFHSHMKNKMEDENVKKYDLFETEHTGLDKLAFEGLSRKDQQIVWSKHYMCERLGRDFYDQYVRIGILVEEEVLDISDDSNAIISEHIQYKTEVRFYHKLFCEWYAAHHLSEYASRDDVTFDPWEESEESEYFDSDSVNDDNDDEPGYRYNVESQTDHYLKYLDPFDLQYVYRFTCGLNRKAADKIIEYLKTREEEGKFAILCILERSGKVEDVLEDVEDLCSELIQINSGDTLLLQRSIIQLLEIATANKVSISGHSGRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1B9X5</accession>
    <name>A0A9Q1B9X5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Paraneoplastic antigen Ma1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_42940</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000168">
        <property type="protein sequence ID" value="KAJ8018845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1B9X5"/>
    <dbReference type="OrthoDB" id="115435at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026523">
        <property type="entry name" value="PNMA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048270">
        <property type="entry name" value="PNMA_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23095:SF46">
        <property type="entry name" value="GAG PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23095">
        <property type="entry name" value="PARANEOPLASTIC ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14893">
        <property type="entry name" value="PNMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Paraneoplastic antigen Ma-like C-terminal" evidence="2">
        <location>
            <begin position="160"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="393"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="445"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="451"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="478"/>
            <end position="508"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8018845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="578" mass="64856" checksum="DE84C080349BFFF0" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BBA5</accession>
    <name>A0A9Q1BBA5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_43870</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000512">
        <property type="protein sequence ID" value="KAJ8018242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BBA5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8018242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="108" mass="11890" checksum="7DE540F9AF5F22EA" modified="2023-09-13" version="1">MTKIFSYDLNRAIHGLGLGFKFLGSTKILQRASQNSEEKHFVETLRVCWYQFGGCFGQNFHGMLSTHGNQLLGGGGTAPSQFRKESCSVCPSLLSRALIKMVGMCLVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BDB8</accession>
    <name>A0A9Q1BDB8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_35989</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BDB8"/>
    <dbReference type="OrthoDB" id="5667at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8023526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="205" mass="22466" checksum="CF8AF94AC3CF3566" modified="2023-09-13" version="1">MSLGFSQNVAVFLSTAGGVGGFVGKTLVVVVFHLDKMNIISAGLIPSVICCIGLTGYILTRDYFLLLICSSFCGFSLAFGDSALSAMLPGYLCKEHLKQGTSLSFLFGGICMQLGGILSGKRWSISDTIGVPSNLKDNEKSPQIYFKISPTFYIFHLISLKNIFYDVSKQTFVITCKKVVILHKLNISVCKLYQFVCYLNPYSLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BDE5</accession>
    <name>A0A9Q1BDE5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Transmembrane protein 47</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_38483</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TMEM47 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BDE5"/>
    <dbReference type="OrthoDB" id="8655982at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005911">
        <property type="term" value="C:cell-cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015664">
        <property type="entry name" value="P53_induced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14399:SF5">
        <property type="entry name" value="CELL JUNCTION PROTEIN VAB-9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14399">
        <property type="entry name" value="P53-INDUCED PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13903">
        <property type="entry name" value="Claudin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0965">Cell junction</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="96"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="124"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="167"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8021318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="204" mass="22643" checksum="AFB857A45B27E0D6" modified="2023-09-13" version="1">MDLSETTETTVTTRVIRPFKIIATILVVIAIILMIPVLVSYVWIQAQTYHQGLWIECYSGLRIVPTNGPGEPTPSTSSEIKCYGAQPSGWLKAAEAFTIISLVLSVFGLLCALLALWKGKQYAIYFTIGGVLLIFSALCILIVLIIFPVKFMVEEVVPGRGVWELGWAWGLALGILVIQLVSGLLYIFAPDREEIYYSEKTYFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BDZ9</accession>
    <name>A0A9Q1BDZ9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_36990</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8024291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BDZ9"/>
    <dbReference type="OrthoDB" id="6119297at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="125"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8024291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="159" mass="17458" checksum="4DFBF7F2712EDD28" modified="2023-09-13" version="1">MIGACQEGEEIEISQCCIQSSHIVGLLSSAVESVPVKEGHISNSMVKVVRDTGCNTVIAREKFVDESCLTGEVRTLKLLNGTVRKCPCAMIEIDTPFFQGVVHALVVQDPLFDLFIGNIEGARDPNDPDPNWNVSDFSDEKPFRGAVETRSQKLKRGNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BFX5</accession>
    <name>A0A9Q1BFX5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Cytochrome b561</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_35193</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8025085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BFX5"/>
    <dbReference type="OrthoDB" id="907479at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1770:FF:000001">
        <property type="entry name" value="Cytochrome b reductase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043205">
        <property type="entry name" value="CYB561/CYBRD1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006593">
        <property type="entry name" value="Cyt_b561/ferric_Rdtase_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10106">
        <property type="entry name" value="CYTOCHROME B561-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10106:SF0">
        <property type="entry name" value="LD36721P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03188">
        <property type="entry name" value="Cytochrom_B561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00665">
        <property type="entry name" value="B561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50939">
        <property type="entry name" value="CYTOCHROME_B561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="4" id="KW-0349">Heme</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="47"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="115"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="152"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="192"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b561" evidence="12">
        <location>
            <begin position="11"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8025085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="250" mass="28335" checksum="77769A5E935283C6" modified="2023-09-13" version="1">MPRFFYPLLALSQLLAVILLVLTGYWTGHMLGGFAWDGSLQQFNLHPLLMILGLVFLYGDAILMFRIFRKENKTVVKVIHMTMHVCSMICIVIALKAVFSYHRDQHLATFFSAHSWIGFLTVLMFFMQWLFGFVSFFLPWLNSDKRIDLLPFHRFFGIAIFAMSCATAVVGVNEKLLLALSSDYSKMGPIATVGNLVALVLVAFALVVGFLVTYAPYQRESTPDIEKLPLTQAASMRDFQATDDDRVLEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BGI8</accession>
    <name>A0A9Q1BGI8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_36333</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BGI8"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8023792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="79" mass="9452" checksum="33E61499F2DFE2F5" modified="2023-09-13" version="1">MPSRDLSLRFHSFIHSFIHSFIHSFIHSFIHSFIHSFIHSFIHSFIHSSSFIHSFVHSFIHLHSVIHSFIHLLVHSFIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BGJ9</accession>
    <name>A0A9Q1BGJ9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">G-protein coupled receptor moody</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_34907</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BGJ9"/>
    <dbReference type="OrthoDB" id="10055255at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00637">
        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24228">
        <property type="entry name" value="B2 BRADYKININ RECEPTOR/ANGIOTENSIN II RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24228:SF72">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="20"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="91"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="140"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="190"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="38"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8024873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="243" mass="27415" checksum="28595894498DD305" modified="2023-09-13" version="1">MEGTSLANTLEGSEHIPQIIRIIDATVLSVIAIIGVLGNTLVFVAYTLSYKIRTKTNIFVVNLATADFLTCLFLPFVSWSLLIEVERVDGWLDLLCGLAMGLIQVFAACSMVTIFSIGVNRYILITRSRHTYNKLYRIKLITLWIFFSWIYSILVITVPLMFNIGQLGFDIKIHACGTKTDHKLAHYYDLILTITLAPLPGTVTACYAGIYLYVRKHNKRILETYGLTRKTNGCHLSSELNKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BGW4</accession>
    <name>A0A9Q1BGW4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Post-GPI attachment to proteins factor 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_35465</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in the lipid remodeling steps of GPI-anchor maturation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Golgi apparatus membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the PGAP3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8025293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BGW4"/>
    <dbReference type="OrthoDB" id="419770at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007217">
        <property type="entry name" value="Per1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13148">
        <property type="entry name" value="PER1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13148:SF0">
        <property type="entry name" value="POST-GPI ATTACHMENT TO PROTEINS FACTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04080">
        <property type="entry name" value="Per1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="3 8" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0732">Signal</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040543910" description="Post-GPI attachment to proteins factor 3" evidence="8">
        <location>
            <begin position="19"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="105"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="134"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="168"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="194"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="231"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="260"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU365066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8025293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="326" mass="37778" checksum="3C8FABD8471A0386" modified="2023-09-13" version="1">MAHLHMFLILCTIPVAIASIGDGNQVFQEFLKGCRSKYCSTDSLVKKFEQNRPVSLSLLRWDCDSECKYSAMWQTVERLQAKNLKIQQFYGKWPFIRIFGIQEPASAFFSLLNGFGHVYMILKLRKQVPRSAPMFYAWHVYSAISIHAWFWSTVFHARDTTLTEKMDYFSAFSLNLSGILMISIRSLAIQEKGTNLWVVAAAFIVLGTIFWRHVSFLNNAKRFDYGYNMKVNLALGLLNSIWWVAWCLPNIYRKPYLKKAIICIVAADLLLLLELGDFPPLFWTFDAHSLWHAGTFPLIFVWYSHCIADCLRLHQPQFLPKYGNVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BGZ7</accession>
    <name>A0A9Q1BGZ7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Polypeptide N-acetylgalactosaminyltransferase</fullName>
            <ecNumber evidence="16">2.4.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Protein-UDP acetylgalactosaminyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">HOLleu_34793</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">Nanhai2018</strain>
            <tissue evidence="19">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 16">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 16">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 16">Golgi apparatus membrane</location>
            <topology evidence="2 16">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 16">Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="16"/>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BGZ7"/>
    <dbReference type="OrthoDB" id="5988548at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
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        <property type="term" value="C:Golgi membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0004653">
        <property type="term" value="F:polypeptide N-acetylgalactosaminyltransferase activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="pp-GalNAc-T"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Polypeptide N-acetylgalactosaminyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045885">
        <property type="entry name" value="GalNAc-T"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675:SF133">
        <property type="entry name" value="GLYCOSYLTRANSFERASE 2-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675">
        <property type="entry name" value="N-ACETYLGALACTOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00652">
        <property type="entry name" value="Ricin_B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00458">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="Ricin B-like lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 16" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="12 16" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 16" id="KW-0430">Lectin</keyword>
    <keyword evidence="15 16" id="KW-0464">Manganese</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6 16" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Ricin B lectin" evidence="18">
        <location>
            <begin position="545"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="37"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="73"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="94"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU361242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAJ8024784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="687" mass="78820" checksum="4F6CAE3DE18BA45F" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BHB7</accession>
    <name>A0A9Q1BHB7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">WAS/WASL-interacting protein family member 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_34735</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BHB7"/>
    <dbReference type="OrthoDB" id="6157464at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003124">
        <property type="entry name" value="WH2_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02205">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00246">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51082">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WH2" evidence="2">
        <location>
            <begin position="28"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="121"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="137"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="161"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="253"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="291"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="314"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="334"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8024737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="444" mass="44711" checksum="F8A0EE7966F3B646" modified="2023-09-13" version="1">MPPPPPPPPGPPPPPSGKKKGGPTSGAGRGALLASIQKGARLKKTETNDRSAPVVGNVKPSPGGGGGGGGGGGGGGGFGGSPAGGLGGLFAGGMPRLKPTGSSRPGNASSSVRPPGPRPPVSNSGPSRPPVSSSGPPRLPASNPGPPRMPTNSFPRSSSGPPRPGQNSAPPPPPPSRNQRGPRSAVPPPPPPNQTSRLSNGNLPPPPPPAGNRVNHSSAQFPPPPPPDNRPNRSSGAFPPPPPGIGRQRDSNLPPPPPPNRNSGVQPPPPPPPNRPVPGGGGGGRRSNANVPPPPPPPMRDPPPISNGPSGNGAPPPPPPMRNPPSSASKNSSRPPPPIRDPPRANPPPPPPSRNPGRPTPPPPPGRNNPPPLPPDRTSRISLVNGDIPGAAFENRFKFSNPNQFPKPPAFKKTAKSYPTKVHAHRQRRGGTQSRGAPPSPPRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BHZ9</accession>
    <name>A0A9Q1BHZ9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">coproporphyrinogen oxidase</fullName>
            <ecNumber evidence="4">1.3.3.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_31899</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; protoporphyrinogen-IX from coproporphyrinogen-III (O2 route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the aerobic coproporphyrinogen-III oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.3.3" evidence="4"/>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BHZ9"/>
    <dbReference type="OrthoDB" id="15318at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004109">
        <property type="term" value="F:coproporphyrinogen oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1500.10:FF:000002">
        <property type="entry name" value="oxygen-dependent coproporphyrinogen-III oxidase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1500.10">
        <property type="entry name" value="Coproporphyrinogen III oxidase, aerobic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001260">
        <property type="entry name" value="Coprogen_oxidase_aer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036406">
        <property type="entry name" value="Coprogen_oxidase_aer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018375">
        <property type="entry name" value="Coprogen_oxidase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003727">
        <property type="entry name" value="PRK05330.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10755">
        <property type="entry name" value="COPROPORPHYRINOGEN III OXIDASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10755:SF0">
        <property type="entry name" value="OXYGEN-DEPENDENT COPROPORPHYRINOGEN-III OXIDASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01218">
        <property type="entry name" value="Coprogen_oxidas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000166">
        <property type="entry name" value="Coproporphyri_ox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00073">
        <property type="entry name" value="COPRGNOXDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102886">
        <property type="entry name" value="Coproporphyrinogen III oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01021">
        <property type="entry name" value="COPROGEN_OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8026928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="376" mass="43135" checksum="329DD7E1E8C2D23D" modified="2023-09-13" version="1">MASWIIGNMSRYSGLVCVGVGTFVIGAGTIHRQRSQCVLQAKTIFPKNNVDLSKYMARPVTSVEKLQETSSDMKSRMEVFILEKQKEICDALQDIEGDIKFKVDRWDRAKGGGGITCILEDGQVFERAGVNISVVHGTLPAKTAQQMRARGRPLDATKPLPFFAAGISSVIHPRNPMIPTLHFNYRYFEVQEEGKTVWWFGGGTDLTPYYLDREDVHHFHKTLKNACDKHDSSYYENFKKWCDNYFVVQHRGERRGVGGIFFDDVDTPDQESVFSFVKECANAVLPCYLPIINKHKNDGYGYHEREWQLLRRGRYVEFNLVYDRGTKFGLLTPNARIESILMSLPKTARWQYMHEPEDGSKEALLMEVLKNPEDWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BIP2</accession>
    <name>A0A9Q1BIP2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_32432</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BIP2"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8027318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="80" mass="8901" checksum="1D6F7D0E92237679" modified="2023-09-13" version="1">MLIILCYCSLIPKEKTPTLLRINATQRNTYYRGPRAVLNSRISQTILDKTLGTLCHNILESPFPGLHGAPAPPSKVAQRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BJQ6</accession>
    <name>A0A9Q1BJQ6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Short coiled-coil protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_30002</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Positive regulator of amino acid starvation-induced autophagy.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Golgi apparatus</location>
            <location evidence="4">trans-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SCOC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8027910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BJQ6"/>
    <dbReference type="OrthoDB" id="2163284at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000038">
        <property type="entry name" value="Short coiled-coil protein a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019357">
        <property type="entry name" value="SCOC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21614:SF0">
        <property type="entry name" value="GEO08385P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21614">
        <property type="entry name" value="SHORT COILED COIL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10224">
        <property type="entry name" value="DUF2205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="89"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="44"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="109"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8027910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="120" mass="13811" checksum="A434FAC0334D0F42" modified="2023-09-13" version="1">MENKSEVIPSDLPNNLAEMSISNTDMSPEDGEDAKEKTRLICQVLELQNTLDDLSMRVDKVKEENLKLKSENQVLGQYIENLMSASSVFQSTSPKNKKKRVSKKSVSPEHQNWQGWWWQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BJR1</accession>
    <name>A0A9Q1BJR1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Arfaptin-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_29958</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">trans-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8027879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BJR1"/>
    <dbReference type="OrthoDB" id="9994780at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032588">
        <property type="term" value="C:trans-Golgi network membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070273">
        <property type="term" value="F:phosphatidylinositol-4-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019904">
        <property type="term" value="F:protein domain specific binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034315">
        <property type="term" value="P:regulation of Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07660">
        <property type="entry name" value="BAR_Arfaptin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000003">
        <property type="entry name" value="arfaptin-2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010504">
        <property type="entry name" value="AH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030798">
        <property type="entry name" value="Arfaptin_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12141:SF5">
        <property type="entry name" value="ARFAPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12141">
        <property type="entry name" value="ARFAPTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06456">
        <property type="entry name" value="Arfaptin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01015">
        <property type="entry name" value="Arfaptin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50870">
        <property type="entry name" value="AH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AH" evidence="7">
        <location>
            <begin position="186"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="28"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="45"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="93"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="110"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8027879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="407" mass="44552" checksum="CE2EA5F1C49D696C" modified="2023-09-13" version="1">MAGEGYPNPVAEENGSIQAEDMDDTFEQDLRNMIRDGPRVGPGGSVVPPSSLHVPGQFTGIDLSSPQKTNITSPNITENPTVGSRLANPSGGADQQETTPPETSRIGMMGSSSNSGSAATTPSSQTGGYNPGGQDNFGNQGGGSYGGQSTSAMAKAKLDSLKVWSVSTYKCTRQMVQERLGKASRTVDLQLEAQIEVLRDTQRKYSNILKLAKTLTSQFYLVVQTQRALGDAFGELAAKSPELQEEFSYNSDTQKSLCSNGEILLSALNFFTSSVNTLCNKTMEDSLITVKQYESARIEYDAYRADLESLQLGPKDATTQAKLHESQLKYDKQKQKFDKLRQDVSVKLKFLDENKVKVMHKQLLLFHNAIAAYFSGNQKALEATLKQFNIRPKVPGEDRPSFLEQHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BKJ4</accession>
    <name>A0A9Q1BKJ4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_30342</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BKJ4"/>
    <dbReference type="OrthoDB" id="414730at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33776">
        <property type="entry name" value="ENDO/EXONUCLEASE/PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33776:SF4">
        <property type="entry name" value="ENDONUCLEASE_EXONUCLEASE_PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8028175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="161" mass="17956" checksum="5606BB25B71FEA1E" modified="2023-09-13" version="1">MSIFNDTIESLFIEISDPSVKDKVIIGIIYRTPDGAVNDFISHFNDVLNTIRSQNCHAYIMGDFNIGMLNSVSSTSFLNTLMSHGFEPTTNKPKMVSGTSATLIDNIFTNINGSMMSGIFVTELSDHFPVFLKIIKEECYQNGSITFRNYTSVTKTSFPQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BLC1</accession>
    <name>A0A9Q1BLC1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Vascular endothelial growth factor A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_31060</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the PDGF/VEGF growth factor family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BLC1"/>
    <dbReference type="OrthoDB" id="8878063at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070851">
        <property type="term" value="F:growth factor receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051781">
        <property type="term" value="P:positive regulation of cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008284">
        <property type="term" value="P:positive regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000072">
        <property type="entry name" value="PDGF/VEGF_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11633:SF1">
        <property type="entry name" value="LD28763P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11633">
        <property type="entry name" value="PLATELET-DERIVED GROWTH FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00341">
        <property type="entry name" value="PDGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00141">
        <property type="entry name" value="PDGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50278">
        <property type="entry name" value="PDGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0339">Growth factor</keyword>
    <keyword evidence="3" id="KW-0497">Mitogen</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Platelet-derived growth factor (PDGF) family profile" evidence="5">
        <location>
            <begin position="109"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8028735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="309" mass="34855" checksum="75E6E02ED1084E28" modified="2023-09-13" version="1">MKFAQGEIEKVFIVTLVISWTSNFGETKIPSSILYEMKDIHSLPELLRTLPINAEDGIHMISTTDVGTPREPQGSVHVYIRNQVIPQEQFPGGPISNNSDQVNGIPINITAREVDVPALPACQPRPTIVKTDTLISTNDDILFWPHCLSVNKCDGCCNTDLFECVSTRTRELPTQLIRMEYDSLTNRLAWAGVTTVGVEEDVGCACKCKVRPHQCRVDREVFNNCQCHCVAQLECPRNFRWDPVTCDCVCDVPESHRLCRSKRETFSSEECSCTCDPEVRERCSRKGLRFNTNKCKCISRSRFEVSDSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BLG5</accession>
    <name>A0A9Q1BLG5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Platelet glycoprotein 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="31">Glycoprotein IIIb</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="29">PAS IV</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="30">PAS-4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="28">Platelet glycoprotein IV</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="32" type="ORF">HOLleu_31185</name>
    </gene>
    <organism evidence="32 33">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="32" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="32">Nanhai2018</strain>
            <tissue evidence="32">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>(9Z)-octadecenoate(out) = (9Z)-octadecenoate(in)</text>
            <dbReference type="Rhea" id="RHEA:33655"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="2">
            <dbReference type="Rhea" id="RHEA:33656"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(9Z,12Z)-octadecadienoate(out) = (9Z,12Z)-octadecadienoate(in)</text>
            <dbReference type="Rhea" id="RHEA:45264"/>
            <dbReference type="ChEBI" id="CHEBI:30245"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:45265"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>butanoate(out) = butanoate(in)</text>
            <dbReference type="Rhea" id="RHEA:45248"/>
            <dbReference type="ChEBI" id="CHEBI:17968"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:45249"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>hexadecanoate(out) = hexadecanoate(in)</text>
            <dbReference type="Rhea" id="RHEA:45256"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="3">
            <dbReference type="Rhea" id="RHEA:45257"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>tetracosanoate(out) = tetracosanoate(in)</text>
            <dbReference type="Rhea" id="RHEA:45260"/>
            <dbReference type="ChEBI" id="CHEBI:31014"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:45261"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>tetradecanoate(out) = tetradecanoate(in)</text>
            <dbReference type="Rhea" id="RHEA:45252"/>
            <dbReference type="ChEBI" id="CHEBI:30807"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="4">
            <dbReference type="Rhea" id="RHEA:45253"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Apical cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Cell membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Membrane raft</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the CD36 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="32">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BLG5"/>
    <dbReference type="OrthoDB" id="514335at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005044">
        <property type="term" value="F:scavenger receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005428">
        <property type="entry name" value="CD36/SCARB1/SNMP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002159">
        <property type="entry name" value="CD36_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11923:SF12">
        <property type="entry name" value="PLATELET GLYCOPROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11923">
        <property type="entry name" value="SCAVENGER RECEPTOR CLASS B TYPE-1 SR-B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01130">
        <property type="entry name" value="CD36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01610">
        <property type="entry name" value="CD36ANTIGEN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01609">
        <property type="entry name" value="CD36FAMILY"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="17" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="23" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="25" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="19" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="14" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="20" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="26" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="21" id="KW-0472">Membrane</keyword>
    <keyword evidence="22" id="KW-0564">Palmitate</keyword>
    <keyword evidence="24" id="KW-0675">Receptor</keyword>
    <keyword evidence="33" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="12" id="KW-0813">Transport</keyword>
    <keyword evidence="16" id="KW-0832">Ubl conjugation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00000934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00000996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00001892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00004285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00010532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00020772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00023949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00029966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00031821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00032188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00032780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="EMBL" id="KAJ8028832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="93" mass="10245" checksum="9415E8A66A7A527B" modified="2023-09-13" version="1">MASRGCLIFLAILGALLVILGGILIPIGNNAIYNGVKTGTVLTPDSEAYDAWVEPPVPVYISYWVWHLKNPDQFLAGQKPVLEQKGPYTYRLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BLK3</accession>
    <name>A0A9Q1BLK3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Glycosylated lysosomal membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_28215</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Required to protect lysosomal transporter MFSD1 from lysosomal proteolysis and for MFSD1 lysosomal localization.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Interacts (via lumenal domain) with lysosomal protein MFSD1; the interaction starts while both proteins are still in the endoplasmic reticulum and is required for stabilization of MFSD1 in lysosomes but has no direct effect on its targeting to lysosomes or transporter activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Lysosome membrane</location>
            <topology evidence="9">Single-pass type I membrane protein</topology>
            <orientation evidence="9">Lumenal side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GLMP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8028953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BLK3"/>
    <dbReference type="OrthoDB" id="6264340at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029382">
        <property type="entry name" value="NCU-G1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31981">
        <property type="entry name" value="GLYCOSYLATED LYSOSOMAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31981:SF1">
        <property type="entry name" value="GLYCOSYLATED LYSOSOMAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15065">
        <property type="entry name" value="NCU-G1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0458">Lysosome</keyword>
    <keyword evidence="5 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0732">Signal</keyword>
    <keyword evidence="2 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040315253" evidence="12">
        <location>
            <begin position="29"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="374"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8028953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="419" mass="46390" checksum="EACB0CB86036972F" modified="2023-09-13" version="1">MSGSRRILGQGLVLGTVVLLHFGQLVKGTEDLTRNVSSTFNEGCNIPFCKNSNNGSFVNVFHISARGQNDTIHFVWSSYGALTGLVAQTKNTAVFRIDWEKVKNRSLNAISFEPKGAVLFSSGVIFSRLIEFDDVSDTAKLTPASKIKVKSFEDLQWNNFNMTVSKTKHSGVFNSSSLAKGGNGFISLMVQGFQDNGRGKVLPQLLHTSNSSYFEFALKNISTHFNNSRFALELVFVHQDKKNKTSFSISRSIADQYTPAVFYVDNVKFTPSSKDNLGGYVSWKPVTYTKEERHLGNGKLVHQYGLKTNVTQLWDHLPLYSIAHAFWGDLKDLDITSMNISFGIDQDGFYKETNYTVWCGLVGYGEAPLDGLSLLIIIVISASIGIPVVLIVFGGSFMSFRNLLRKKKGEALLVDPGIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BMD4</accession>
    <name>A0A9Q1BMD4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_28553</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BMD4"/>
    <dbReference type="OrthoDB" id="10671371at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053282">
        <property type="entry name" value="RGS_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47079">
        <property type="entry name" value="REGULATOR OF G-PROTEIN SIGNALING PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47079:SF1">
        <property type="entry name" value="REGULATOR OF G-PROTEIN SIGNALING PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="24"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="58"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="165"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8029214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="390" mass="43725" checksum="B91A7AAB5D79601C" modified="2023-09-13" version="1">MKREVDDADAQAPRCPSATPFQRSSISTPSSLTVSKSSLSNSSQSIQTPSRRRVSMTSASHHGSSSSSIGSSSRVAAGTTTKKVTKPPRRSRAMSISFRLADFSFMMEGDDEEGGEKDKKDSIPALPQLDIQALQDVRSKAVEKRKQEERKRLAEMRKKKKKSKKEAALASDEKQDKDKKKVKKSKDTPGKKGKSQKEKESHIAKLTKGNLEKFDLQNSYQTRYPLRRGSLVGTETERRRRLSTSFSIDSRNTLSSDLYHTETLSASVEQEVEKLPLVDQAPTESLTFDHMDSDYTSSKASMSHSSYRQISGAILADRVAGRPWETFLKKQGKFYDLHHLQFWQTAQELLTLQDTKQGNETLSSARQLVIQRITALYLLPSGNLSLWSIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BNT1</accession>
    <name>A0A9Q1BNT1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_26533</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BNT1"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040508847" description="Ig-like domain-containing protein" evidence="3">
        <location>
            <begin position="28"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="275"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="30"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="133"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="376"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="657"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="657"/>
            <end position="675"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8030198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="691" mass="75829" checksum="2C5A46DF5CA612AB" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BSG7</accession>
    <name>A0A9Q1BSG7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Synaptotagmin-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_25045</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BSG7"/>
    <dbReference type="OrthoDB" id="67700at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070382">
        <property type="term" value="C:exocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017156">
        <property type="term" value="P:calcium-ion regulated exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00276">
        <property type="entry name" value="C2B_Synaptotagmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10024">
        <property type="entry name" value="SYNAPTOTAGMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="2">
        <location>
            <begin position="142"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="2">
        <location>
            <begin position="274"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8031744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="421" mass="47056" checksum="5E6E598AB39E7470" modified="2023-09-13" version="1">MTLRADPSVIILMIVTLTLGSMLGAILLLCVLYRIMRYYRPHIKTKKSEKEKTPSQTSAMPLMSVAGESWEKISLDSGTVTSKLISGDYGSIQTGLSTVPDSPASLDTFQFDSSITDGSELVSPGEEIRFLNLPQVDQKAGEIGTIEFSTKFTNAKGLTITVHSVEKLPKKEIGGTTDPYVKVQLVKDDTRKSPEFEFQTPTKKDTESPIFEQSFTARIAYPNIHDYFLKLIVCDYSKYFDVDAIGEVKLPLDDIEPDFTIRSTLELTPSMSDSKGDVLVSLSYLPTSKRLLVTVLQARGLAMPEDKIENVETYARIVLIVGHRKIRRKKTMASKTGKNVSFDERFYFDIPMEKLEKIKLMIIVTNGRDSGYDSESGLGGPEVEIGKVLIGHSCSTKAHAHWKEMLLFPRIQIAQWYTLRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BUX8</accession>
    <name>A0A9Q1BUX8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Carnitine O-acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_23174</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BUX8"/>
    <dbReference type="OrthoDB" id="240216at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.70">
        <property type="entry name" value="Choline/Carnitine o-acyltransferase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000542">
        <property type="entry name" value="Carn_acyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039551">
        <property type="entry name" value="Cho/carn_acyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042231">
        <property type="entry name" value="Cho/carn_acyl_trans_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22589:SF103">
        <property type="entry name" value="CARNITINE O-ACETYL-TRANSFERASE, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22589">
        <property type="entry name" value="CARNITINE O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00755">
        <property type="entry name" value="Carn_acyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Choline/carnitine acyltransferase" evidence="2">
        <location>
            <begin position="2"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8033049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="86" mass="9989" checksum="30A12C4223CD6890" modified="2023-09-13" version="1">MVEEFCKPGGTGERLQEALQRRADLKENWLSDWWRIHYLQVRDPLVLTSSPCFLFHRQEFSGVTGQIEYAAKLIFSTAQFASLLAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BVK6</accession>
    <name>A0A9Q1BVK6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Syntenin-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_23854</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BVK6"/>
    <dbReference type="OrthoDB" id="10059177at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06721">
        <property type="entry name" value="PDZ1_syntenin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06794">
        <property type="entry name" value="PDZ2_syntenin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000043">
        <property type="entry name" value="Syntenin-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051230">
        <property type="entry name" value="APP-Binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12345:SF3">
        <property type="entry name" value="PDZ DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12345">
        <property type="entry name" value="SYNTENIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PDZ" evidence="2">
        <location>
            <begin position="109"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="2">
        <location>
            <begin position="193"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8033565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="293" mass="31399" checksum="BB2701A8F6FCEDE2" modified="2023-09-13" version="1">MSSLYPSLEDMKVDQMAQAQASFNQQAHQAITASQAQPGSYSSLYPSLSDWMGLDLNSTDIALTNAQQPADQSQAVVTRQSGTVSNYVAPISGENLGIKRAEIKQGVREVILCKGSDGKVGVRFQAISKGVFVSFVGSGTPAALGGLRFGDQILQINGENTAGWSKSKAMEVMKKADPKKIVLAIRDRPFERTITLQKDSAGYVGFVYKNGKITAIGKDTSAARNGLLIDHALLEVNGQNVIGVKDKKIGEVIQNVGKTVTVTVMPNFVFDHMMKSMSSSLVKGSMDHSVPEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BVS1</accession>
    <name>A0A9Q1BVS1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA-directed DNA polymerase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_20961</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BVS1"/>
    <dbReference type="OrthoDB" id="6153129at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33206:SF1">
        <property type="entry name" value="DNA-DIRECTED DNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33206">
        <property type="entry name" value="PROTEIN CBG10425"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8034218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="175" mass="20461" checksum="009E1CB65C5A36D6" modified="2023-09-13" version="1">MLEFYYDFMVKFVSTADFEYCEMDTDSAYLAISGDSLDDVIKPDMRTAFDLEKHLWFPRTDTDEHKQYDKRTPGLFKLECEGNGIVALNSECYYCFGSEKEKVSCKGVYNRSTHLTKDNYLKVLRTCSSVEAQNMGFRLWQNQMQTYIQEKTALSYLYIKRKVNEDGVSTRPLDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BWB2</accession>
    <name>A0A9Q1BWB2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_24348</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BWB2"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="78"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8033955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="135" mass="16054" checksum="A8DC2BFEB0999ED3" modified="2023-09-13" version="1">MPFNLDKFVASPSVEELDSLKKSEIVKVAKHYGIEFQPLMRKDEIKRYVLEYLVDEGVLPSTVLETAITVPTDNTFELKRLEIEMNKEKRKKRKEKCKCKRKKRKEKCKCKRKKRKEKCKCKCKGKKRQENMNLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BWE3</accession>
    <name>A0A9Q1BWE3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Alpha-N-acetylgalactosamine-specific lectin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_24216</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BWE3"/>
    <dbReference type="OrthoDB" id="418245at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050111">
        <property type="entry name" value="C-type_lectin/snaclec_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22803">
        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C-type lectin" evidence="1">
        <location>
            <begin position="84"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8033849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="226" mass="25702" checksum="D9B6FCC2FC8E45A0" modified="2023-09-13" version="1">MTTQIRKRSGRKLGTRNLQRSSLFWLGLSSNSEGSFQEESVFAKSYSMAFSVFKALLLVSCFAHLSSGSTDSCFLGCPSFWTLFNGYCYRFFGQRVSWQEANYNCQTQGGWYTFANLVSIHSKKENEFVSNMFKSYTDSIDPTGPGHEIWIGLTDQEEEGTFVWTDGTAADFTAWKSDEPNGGSREQAVVMGHPNTVYFAEWYDVVLETRRCRYICKMRCLGCCKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BX93</accession>
    <name>A0A9Q1BX93_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Tyrosine kinase receptor Cad96Ca</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_22104</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BX93"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004714">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048468">
        <property type="term" value="P:cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050793">
        <property type="term" value="P:regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00192">
        <property type="entry name" value="PTKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001512">
        <property type="entry name" value="Receptor tyrosine-protein kinase erbB-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050122">
        <property type="entry name" value="RTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416:SF621">
        <property type="entry name" value="TYROSINE KINASE RECEPTOR CAD96CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="1"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8035028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="169" mass="19293" checksum="16ECAED9E0B95EE1" modified="2023-09-13" version="1">MEYLENGSLLNYLTSFSERPTRLSVMPTENDGTMITSKELMRFGLEIAKGMEYLASKNLVHRDLAARNILLGDNLQCKVSDLGLARILKYSNEYEMRSKSRVPVRWMAPESILRNKYSTTSDVWSFGVVLWEIATLGSHPYPGLGCKQIIDDVKKGMRLLQPTHCRDEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BZK5</accession>
    <name>A0A9Q1BZK5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">E3 ubiquitin-protein ligase TRIM56</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_22543</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BZK5"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011044">
        <property type="entry name" value="Quino_amine_DH_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047153">
        <property type="entry name" value="TRIM45/56/19-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25462">
        <property type="entry name" value="BONUS, ISOFORM C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25462:SF291">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50969">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="18"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="7">
        <location>
            <begin position="94"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="304"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8035346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="713" mass="82815" checksum="7763C465A8CBA23A" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C2B1</accession>
    <name>A0A9Q1C2B1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">WD repeat-containing protein 13</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_17548</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8036890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C2B1"/>
    <dbReference type="OrthoDB" id="1932312at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990841">
        <property type="term" value="F:promoter-specific chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051350">
        <property type="entry name" value="WD_repeat-ST_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22838">
        <property type="entry name" value="WD REPEAT PROTEIN 26-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22838:SF4">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="200"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="436"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8036890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="484" mass="53144" checksum="1C7B4F16FF96CE4F" modified="2023-09-13" version="1">MASSWQQILAIDARYNAYRTPNSPQFRTLYVKRRSQLLRENAKAGFDSQIRKQYIRQRMQLLADKYGATITLSDQSSLRSRSTSIRGSTTNLSALEDPYGVRSQSRLSSNQLEVPGDGLLPKGLTPTKNAEASRALVGDNTIGENYAFAGMHHIFDQHSKAVTAVKFANDDKSRVACSSLDGTLSVCQVLPSPATVICILKGHSDGVTDFCWSLSNDLLLSSSLDGTVRLWVVSSGKCARIIEDPNKVPVHCCTFQPLNNNLVISGNSRGHVQVLNVSTGKTARGGLGKLPGRVLCLTCNDTGNLLWAGDDKSNLQSFIFDITSGKLSKGKRIVVSEGHPITCLSFRAWISREASDPSLLINCAYNSLCLYRIVSSDGEPRLRKTFSIKHRNENIRSSFCPLMSFREGACVVTGSEDMSVYFFDIERTTKPCVNKLQGHSAPVLAVSFNYDESLLASSDSDGLVIVWKRQQNAEENLKDIQGNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C2E1</accession>
    <name>A0A9Q1C2E1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Lengsin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Glutamate-ammonia ligase domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_17505</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">May act as a component of the cytoskeleton or as a chaperone for the reorganization of intermediate filament proteins during terminal differentiation in the lens. Does not seem to have enzymatic activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Dodecamer. Interacts with BFSP2 and VIM.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the glutamine synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8036859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C2E1"/>
    <dbReference type="OrthoDB" id="77835at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004356">
        <property type="term" value="F:glutamine synthetase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006542">
        <property type="term" value="P:glutamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.70">
        <property type="entry name" value="Glutamine synthetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.590.10">
        <property type="entry name" value="Glutamine synthetase/guanido kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008147">
        <property type="entry name" value="Gln_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036651">
        <property type="entry name" value="Gln_synt_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008146">
        <property type="entry name" value="Gln_synth_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43407">
        <property type="entry name" value="GLUTAMINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43407:SF1">
        <property type="entry name" value="LENGSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00120">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01230">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54368">
        <property type="entry name" value="Glutamine synthetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51986">
        <property type="entry name" value="GS_BETA_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51987">
        <property type="entry name" value="GS_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GS beta-grasp" evidence="8">
        <location>
            <begin position="21"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="GS catalytic" evidence="9">
        <location>
            <begin position="122"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00037583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00039404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00042675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8036859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="457" mass="51977" checksum="FA0EA70CCBACF64A" modified="2023-09-13" version="1">MCAQPISNEALSEVRNRIERDKIEYIFLECSDFHGISRGKVLPVDRFLENSEDGFSIPAMQLIRLPKGDIPPMSNLPLSEEFSDFTFFPDFSTFKVIPWRKGVASVLVDPYTVSGEPVGFFTRHVARHQIERLKDYGLSILSALEYEFSLLDSKTLLPIHSDSTGHWIKNFFDDWDHLTSIHKNLSSMDLVVEQIDTEGEEGVYEFPIKPSLGLKAGDDAYWLKKTVRELSQQKGYLASFSSKPFIHSSGVGGHFNHSLLDLEGKERLFYDVENPHKMSKIMQHWLAGLIRHAPAMLIFGCPTASCQRRFASPDRATPQYPDWGIDNRTCSFRVKNLTPEKTYIENRIFGSACNPYLTLAVNIAAGLDGILNEIPLQRMVKGNAHSVAHVLPGAHKFSGRLSDALWELEQDEVLRQALGEDFIRPYINAKKHECDRETEAYMKGDASFYDREYLAYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C2P4</accession>
    <name>A0A9Q1C2P4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_18062</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C2P4"/>
    <dbReference type="OrthoDB" id="10042460at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="143"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="177"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="239"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="274"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="343"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="377"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="445"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8037282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="487" mass="55711" checksum="FA9034CF389D2968" modified="2023-09-13" version="1">MDHASVVVNGFSEDSIDESKSRHKWPFSFWALLKVLCLFHHRTVVTERKCFLCHVKNISQNNHALENITSAKKYGIIDALKLAYESDGKKIGCGETESLLAKTCNDNECEVCKSEWWNLSRTPYIYTEEEIGVDRWNHRGSSVISIVVLVLLLGLWIWDMSYPFIKLWSSPGHTMELLTEIGLFLFVVSNVVVVLCSKLRSIFMEGCPFLFWASALNVRYIIKRAQFLDLHNKGLPGKLLLAICICWPMFNSCFRAAIFLITTDYNDSVAFFHAVRTSIVCTLLFQCWGCFVYMLYLIRVSFQRQFTLVLSYFVEYEGCIDACRGILLNVVTDFQCFKQLSNLYVVIMLPVAFIGMAANLSWSYLLDQACRENEEVMIVLNCVLFLAWSEMIMGIFLVSYAQGGVDVMYLWDNFFDDVLKVHSDRHEAFWRNLIRSMALISRKEGRLLVASVVFSVIGVYMALSRGNQDISFLVKACNGTNLTSTIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C4Q4</accession>
    <name>A0A9Q1C4Q4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_16136</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C4Q4"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.130.10">
        <property type="entry name" value="Ribonuclease A-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036816">
        <property type="entry name" value="RNaseA-like_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040125114" evidence="1">
        <location>
            <begin position="25"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8038651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="154" mass="18480" checksum="C5CD783C7C9AE060" modified="2023-09-13" version="1">MKNFAVICFLVAATAILFVQRISSDDGFWYGEELHDVEKRQTWNVFCDKHVVEDSSNREDDRFWNRIARQRLRDYGVRLSNTFIRVQNKDLILNSFHRRRECSKGYTKSDDKFTLVEMTWNTDLERYSVNYPGQWHICVKLDANRQPIHFHGKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C5W9</accession>
    <name>A0A9Q1C5W9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Hamartin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_16855</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6022054at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033596">
        <property type="term" value="C:TSC1-TSC2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008285">
        <property type="term" value="P:negative regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032007">
        <property type="term" value="P:negative regulation of TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007483">
        <property type="entry name" value="Hamartin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15154">
        <property type="entry name" value="HAMARTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15154:SF2">
        <property type="entry name" value="HAMARTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04388">
        <property type="entry name" value="Hamartin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="287"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="379"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="483"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="567"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="652"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="701"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1139"/>
            <end position="1165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1177"/>
            <end position="1372"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="890"/>
            <end position="998"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="291"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="379"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="394"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="405"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="499"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="569"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1142"/>
            <end position="1158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1184"/>
            <end position="1193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1194"/>
            <end position="1217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1247"/>
            <end position="1265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1361"/>
            <end position="1372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8039212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1372" mass="152156" checksum="96A849BF946C8F1F" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C6U1</accession>
    <name>A0A9Q1C6U1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_16846</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C6U1"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8039204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="101" mass="12174" checksum="33D60C01BD1B311F" modified="2023-09-13" version="1">MPLPPPDYDGVEVSAELVLDDFGIIEHVTVIVDEAWSIDSQDLKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C7N4</accession>
    <name>A0A9Q1C7N4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_14492</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10 15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C7N4"/>
    <dbReference type="OrthoDB" id="193931at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120025">
        <property type="term" value="C:plasma membrane bounded cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004679">
        <property type="term" value="F:AMP-activated protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043050">
        <property type="term" value="P:nematode pharyngeal pumping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd12122">
        <property type="entry name" value="AMPKA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14079">
        <property type="entry name" value="STKc_AMPK_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14336">
        <property type="entry name" value="UBA_AID_AMPKalpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000079">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000055">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.80">
        <property type="entry name" value="Kinase associated domain 1, KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032270">
        <property type="entry name" value="AMPK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028375">
        <property type="entry name" value="KA1/Ssp2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049020">
        <property type="entry name" value="PRKAA1/2_AID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF110">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16579">
        <property type="entry name" value="AdenylateSensor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21147">
        <property type="entry name" value="AMPK_alpha_AID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103243">
        <property type="entry name" value="KA1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="1"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="455"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="468"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8040256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="510" mass="58420" checksum="7071EAC4289D223F" modified="2023-09-13" version="1">MCPCRLPVASHMVTNHRVAVKIMNRQKIKNLDVVSKIKREIQNLKLFRHPHIIKLYQVISTPSDIFMVMEFVSGGELFDYIVKHGKIKEFEARRFFQQIISGVDYCHRHMIVHRDLKPENLLLDEQLNVKIADFGLSNIMTDGEFLRTSCGSPNYAAPEVISGKLYAGPEVDIWSCGVILYALLCGTLPFDDEHVPTLFRKIKGGAFSIPAHLEKSPVKSLIKHMLQVDPMKRASVRDIRDHEWFKMDLPDYLFPQNNFDDSIVDVEAIREVCEKFSVTDTEVRDALMAGDPHHQLRIAYYLIIDNKRMMSEADKSQMQDFYLASSPPPRDSYLEENLPPIVKVHPERVNVLALNSLDNVHPMAQDIQRRMATPVKRSKWHLGIRSQSRPHDIMSEVYRAMKTLNYEWKVINAFHVHVRRSNPVTGSYSKMSLQLYQVDNKSYLLDFKSLSNTRDGSVGDDKMDIDEPVSPRSPSSPSTPLSPTQPKFGKGCQTMEFFEMCASLIGTLAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C7W9</accession>
    <name>A0A9Q1C7W9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Growth arrest and DNA damage-inducible protein GADD45 alpha</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_14646</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C7W9"/>
    <dbReference type="OrthoDB" id="5976967at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024824">
        <property type="entry name" value="GADD45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10411:SF8">
        <property type="entry name" value="FI09246P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10411">
        <property type="entry name" value="GROWTH ARREST AND DNA DAMAGE-INDUCIBLE PROTEIN GADD45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8040376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="173" mass="19492" checksum="E6670D5C898436B4" modified="2023-09-13" version="1">MTLGEGEYSESMNVAYKLEAGSVLRDVLFQAKLEKRITCGVYQAASKLSMSPKSVMMCIQADDSTRDDVLHMHFALMKAFCWENTIPLLKVSNQGALYELLKDMEVPPDCTSEDEEEAEDGFAERENVAVTFEDTVCVLIEVQKEKTELENVLLHHHHSMTQFTPSPTIRLAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C8U3</accession>
    <name>A0A9Q1C8U3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">F-box/LRR-repeat protein 12</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_14777</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C8U3"/>
    <dbReference type="OrthoDB" id="3219396at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031398">
        <property type="term" value="P:positive regulation of protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057207">
        <property type="entry name" value="FBXL15_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20933:SF4">
        <property type="entry name" value="F-BOX INVOLVED IN POLYQ PATHOGENESIS, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20933">
        <property type="entry name" value="F-BOX ONLY PROTEIN 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25372">
        <property type="entry name" value="DUF7885"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="F-box" evidence="3">
        <location>
            <begin position="48"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8040477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="352" mass="40319" checksum="82D2F14C05FFAA36" modified="2023-09-13" version="1">MSSKAKQENDYESPAATSNEIVTRPRKVRRQAKCVNTSDRKETDFAEVSRILQLPEMVLLHVFSFLPVEDKCRSSRVCKLFRRLMKDPQLWKSVDLIPCTIRLQALTVFTRRYLSKSLLELSLKGLLWTTAKTSLLSNSFLSELRERCPNLRKFVVLNANLQDIMSSLFPKNVEVLRINYCQVREGWFHSAVEVGNFLNIKCLNITGCGRFTNKDIASLKQMKKLEELNILGCYRLTDQALETIAKDHINLKKLLIGDSDYTNKGILMISQSLKSLRSFTLQNCEFVTGQCLNYILRMTTLEELDISYNPQFNDTHVSMLRKGLPQLKVLRVEEAFAPVQSAGDVNHDDGVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CBD6</accession>
    <name>A0A9Q1CBD6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Noelin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_12451</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CBD6"/>
    <dbReference type="OrthoDB" id="8626508at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003112">
        <property type="entry name" value="Olfac-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050605">
        <property type="entry name" value="Olfactomedin-like_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23192:SF87">
        <property type="entry name" value="AMASSIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23192">
        <property type="entry name" value="OLFACTOMEDIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02191">
        <property type="entry name" value="OLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00284">
        <property type="entry name" value="OLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51132">
        <property type="entry name" value="OLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040173012" evidence="5">
        <location>
            <begin position="17"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="domain" description="Olfactomedin-like" evidence="6">
        <location>
            <begin position="240"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="134"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8041589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="503" mass="56751" checksum="2E78DAD5D86607FE" modified="2023-09-13" version="1">MFVSVTSKLFLAIVMSSIFEPHGNMAFGATVLTEDTESGFAVNSSTNGNPDECVCNVHVPTTIQCTRVTNSATTALIAHLQEQISELTAQVSNHVVLYRAQTERLDSLSATVLTTLDTLGRIEAGTLIVSRPEIVQIREELNDIEAVLLTLRQNNASLAAITQIQAEILNVTTLLDELEIQENADTSDLLRQIEGLKARLQECRDSEVTTALYDLWDQEGKQVNSRHLEPIFIEFSCLDTCSELVAVSLPYTVSGLRPTYGSWFRDPLENRRRVFSYEMPNGRYAQTLNAYRSLKDFHDNRDVTTVSFRPDYSQGTGLVAYNGSLYYNIWNSGNLGRFDVRVNRKLVTKSLPGASYSSEYPYRSGAYSDIDILVDESGLWAVYSSSAANGFILISKLDPDTLDVVQTWQTNYPKNAAGNCFIVCEVLYCTNGFDSHINRINYKYDTKTEAEDFLEIPFDNKFLKTYSLNYNPYDQKLYGWDNGHQMIYDLVFRSHTEEEETQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CCY1</accession>
    <name>A0A9Q1CCY1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HTH psq-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_09918</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CCY1"/>
    <dbReference type="OrthoDB" id="8191755at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007889">
        <property type="entry name" value="HTH_Psq"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05225">
        <property type="entry name" value="HTH_psq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50960">
        <property type="entry name" value="HTH_PSQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH psq-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8043007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="188" mass="21649" checksum="1A2AEEB0B862020E" modified="2023-09-13" version="1">MVRTYKKKTARMKYDTKELNAALERIRDGEMSYGEASKRFGIPKSTLYDQMKTQTGPRMGRQTVLPKSLEDELCQDTLTMSDMFYGLTADALRSLAYELADKNNLKHPFDTEKRKADKDWVYNYLQRYPQLSLRNPGPNSMARAIGFRKTEVDLFYKNLGDVLAKHKLAPSRIYNCDEAGLSSVQKPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-04-02" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CFX0</accession>
    <name>A0A9Q1CFX0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Zinc finger FYVE domain-containing protein 26</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_07262</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1936617at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000281">
        <property type="term" value="P:mitotic cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032465">
        <property type="term" value="P:regulation of cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006869">
        <property type="entry name" value="DUF547"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028730">
        <property type="entry name" value="ZFYVE26"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46591">
        <property type="entry name" value="ZINC FINGER FYVE DOMAIN-CONTAINING PROTEIN 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46591:SF1">
        <property type="entry name" value="ZINC FINGER FYVE DOMAIN-CONTAINING PROTEIN 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04784">
        <property type="entry name" value="DUF547"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF547" evidence="3">
        <location>
            <begin position="1493"/>
            <end position="1663"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="505"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="880"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1023"/>
            <end position="1042"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1211"/>
            <end position="1238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="888"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="900"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="939"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1033"/>
            <end position="1042"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8044497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1689" mass="189220" checksum="65BA7719DAE26BD6" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CGJ7</accession>
    <name>A0A9Q1CGJ7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_07824</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CGJ7"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8044927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="115" mass="12902" checksum="469D812FE9A04B10" modified="2023-09-13" version="1">MARIKRKNRIVFGGGQRSSGVTWGQNLKALFTRYLKLESFDRFHAWYMCCGERKNPIVFGGGQTSSGVTGGQNLKTLFTRYLKMRCGERKNPILFGGGQTSSEVTRGQNLKALFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CH35</accession>
    <name>A0A9Q1CH35_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ABC-type xenobiotic transporter</fullName>
            <ecNumber evidence="3">7.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HOLleu_07106</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Nanhai2018</strain>
            <tissue evidence="17">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O + xenobioticSide 1 = ADP + phosphate + xenobioticSide 2.</text>
            <dbReference type="EC" id="7.6.2.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.2" evidence="3"/>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008559">
        <property type="term" value="F:ABC-type xenobiotic transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18598">
        <property type="entry name" value="ABC_6TM_MRP7_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18605">
        <property type="entry name" value="ABC_6TM_MRP7_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000113">
        <property type="entry name" value="ABC transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000037">
        <property type="entry name" value="ATP-binding cassette subfamily C member 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000997">
        <property type="entry name" value="Multidrug resistance-associated protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000163">
        <property type="entry name" value="Multidrug resistance-associated protein member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF330">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C MEMBER 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
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        <location>
            <begin position="30"/>
            <end position="49"/>
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    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="70"/>
            <end position="90"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="110"/>
            <end position="130"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="142"/>
            <end position="167"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="179"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="374"/>
            <end position="394"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="406"/>
            <end position="428"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="483"/>
            <end position="501"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="507"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="586"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="988"/>
            <end position="1008"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1061"/>
            <end position="1082"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1148"/>
            <end position="1178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1249"/>
            <end position="1270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1276"/>
            <end position="1300"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="16">
        <location>
            <begin position="368"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="15">
        <location>
            <begin position="712"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="16">
        <location>
            <begin position="1000"/>
            <end position="1308"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="15">
        <location>
            <begin position="1344"/>
            <end position="1577"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="700"/>
            <end position="722"/>
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    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="942"/>
            <end position="964"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ8044374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1579" mass="177513" checksum="63D2F57EB06E18E5" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CIH4</accession>
    <name>A0A9Q1CIH4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Lysosome-associated membrane glycoprotein 5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Lysosome-associated membrane protein 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">HOLleu_08563</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="24">Nanhai2018</strain>
            <tissue evidence="24">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Plays a role in short-term synaptic plasticity in a subset of GABAergic neurons in the brain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell projection</location>
            <location evidence="4">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="17">Cell projection</location>
            <location evidence="17">Growth cone membrane</location>
            <topology evidence="17">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Cytoplasmic vesicle</location>
            <location evidence="15">Secretory vesicle</location>
            <location evidence="15">Synaptic vesicle membrane</location>
            <topology evidence="15">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Early endosome membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum-Golgi intermediate compartment membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="20">Membrane</location>
            <topology evidence="20">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Recycling endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 20">Belongs to the LAMP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CIH4"/>
    <dbReference type="OrthoDB" id="6248302at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072594">
        <property type="term" value="P:establishment of protein localization to organelle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048528">
        <property type="entry name" value="Lamp2-like_luminal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002000">
        <property type="entry name" value="Lysosome-assoc_membr_glycop"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11506">
        <property type="entry name" value="LYSOSOME-ASSOCIATED MEMBRANE GLYCOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11506:SF35">
        <property type="entry name" value="LYSOSOME-ASSOCIATED MEMBRANE GLYCOPROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01299">
        <property type="entry name" value="Lamp2-like_luminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51407">
        <property type="entry name" value="LAMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="14" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="8" id="KW-0967">Endosome</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 20 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0770">Synapse</keyword>
    <keyword evidence="6 20 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 22" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="21"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="310"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="Lysosome-associated membrane glycoprotein 2-like luminal" evidence="23">
        <location>
            <begin position="127"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="42"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="42"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="85"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00029428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00053950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00060492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00074379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00076257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF01299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="KAJ8045535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="348" mass="38807" checksum="F5218D1B9F6CE6F6" modified="2023-09-13" version="1">MLKRCERLQNCLNKMARLDCIYAQIWLVLFYLTVAISTDPSSNPVQYDSASSPPLKPPSQSNATSSGPSQLSEKDSTTHPYDQPVETSTVGLSDTSFETSKIKPTTIEETSEKKLIKLEAASPIGQRGEFSLTNEEGEICLKALFEIIFKIYYKSKTADENQVSIPLSAKANADGQCGSDKEDAILILQWDEDRYTLKFTFKKNPKERRWVCERIQLTYDKQNNPDFAGAVNPGICVVHTDEGDTFFGTKLGMSRSCKTEDDKTLISDDKKNKVVMEITRLHLQPFDIVNGTFGEESPCPSDLKKKNNTASLVVGLLLAMFILIVISGYAVGRKMGYITERPGYSTME</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CK57</accession>
    <name>A0A9Q1CK57_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Coiled-coil domain-containing protein 9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_09073</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CK57"/>
    <dbReference type="OrthoDB" id="10058133at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029336">
        <property type="entry name" value="DUF4594"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15635">
        <property type="entry name" value="COILED-COIL DOMAIN CONTAINING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15635:SF12">
        <property type="entry name" value="HABP4_PAI-RBP1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15266">
        <property type="entry name" value="DUF4594"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="4">
        <location>
            <begin position="166"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="63"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="241"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="318"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="386"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="75"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="117"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="142"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="253"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="476"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="506"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="522"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8045949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="533" mass="60024" checksum="C34444A8CD348495" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CKA6</accession>
    <name>A0A9Q1CKA6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Striatin-3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_09176</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat striatin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8046024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CKA6"/>
    <dbReference type="OrthoDB" id="727118at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.300:FF:000001">
        <property type="entry name" value="striatin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013258">
        <property type="entry name" value="Striatin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051488">
        <property type="entry name" value="WD_repeat_striatin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15653:SF0">
        <property type="entry name" value="CONNECTOR OF KINASE TO AP-1, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15653">
        <property type="entry name" value="STRIATIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08232">
        <property type="entry name" value="Striatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Striatin N-terminal" evidence="9">
        <location>
            <begin position="42"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8046024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="107" mass="12579" checksum="D9AF209FB41D9E45" modified="2023-09-13" version="1">MIMDNVGGPAQNHVNQINSGGSSNFNSVQNKSEDGHQRPNYTIPGILHFIQHEWARFEMERAQWEVERAEFQARIAFLQGERKGQENLKRDLVRRIKMLEYALRQER</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CKX9</accession>
    <name>A0A9Q1CKX9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">N-acetylglucosamine-6-sulfatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_05327</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="PTM">
        <text evidence="6">The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sulfatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CKX9"/>
    <dbReference type="OrthoDB" id="96314at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005539">
        <property type="term" value="F:glycosaminoglycan binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008449">
        <property type="term" value="F:N-acetylglucosamine-6-sulfatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030203">
        <property type="term" value="P:glycosaminoglycan metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16147">
        <property type="entry name" value="G6S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012251">
        <property type="entry name" value="GlcNAc_6-SO4ase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024607">
        <property type="entry name" value="Sulfatase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000917">
        <property type="entry name" value="Sulfatase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43108">
        <property type="entry name" value="N-ACETYLGLUCOSAMINE-6-SULFATASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43108:SF8">
        <property type="entry name" value="SD21168P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00884">
        <property type="entry name" value="Sulfatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036666">
        <property type="entry name" value="G6S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00523">
        <property type="entry name" value="SULFATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00149">
        <property type="entry name" value="SULFATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040231909" evidence="7">
        <location>
            <begin position="24"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="domain" description="Sulfatase N-terminal" evidence="8">
        <location>
            <begin position="30"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="modified residue" description="3-oxoalanine (Cys)" evidence="6">
        <location>
            <position position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR036666-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8046595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="504" mass="57122" checksum="CD05A688B6054049" modified="2023-09-13" version="1">MAKLVFHAGYLLVFFATFLKVSAAVAASKPNIIFILTDDQDVQLQGMTPMTKTLDLLGKNGMTFQNMFTVSPLCCPSRSSILTGNYVHNHEAYNNSISGNCSSTTWQQGPEQNTFATQLKSQGYKTFFAGKYLNQYGDPKVGGPEHIPPGWDEWNGLIYNSVYYNYNLSVNGKLEVHKKDYHKDYLTDLIHNRSIDYLNCVSKKESPFFMMLSTPACHRPFDSAPQYMKNFTTKKAPRDKAFNTQAKNKHWLIQQAPHPMSDESLAYVDDAFQKRWRTLLSVDDMVEDIVNTLDKLKLLDSTYIFFASDNGFHLGQFSLPNDKRQMYDFDIRVPLVVRGPGIKANSKSTQPVMTIDLMPTFVHLASGTAPSNVDGQSLVPLLTPKQFTSVTWRKDMLIEHQGEYLVHGTENCTHTEYLNECWPFCICEDSYNNTYSCLRTISGKDNYVLCQFSDAANFQEFYNMTADPYQLTNDVSSLDPAYLDDLSNRLIALSVCKGSTCHKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CMG6</accession>
    <name>A0A9Q1CMG6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Octopamine receptor beta-3R</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_06004</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CMG6"/>
    <dbReference type="OrthoDB" id="10026056at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248">
        <property type="entry name" value="ADRENERGIC RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="307"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="342"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="298"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="23"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="23"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="64"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8047104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="393" mass="45148" checksum="1C71827C067F8D05" modified="2023-09-13" version="1">MFPRRCKRRYDACGVELKTYDSIEEENDDLQDSEDSNDDRCDPLDEYEENEDSMYKNYSRRKSICNSQNSRRSNSVSDISGNGEKSSIAQFQRRCCQGCHEPTMESVVNGNTCSGYEPKPLKMPNVEQIKKFGGVVCPKCSQLFTRIGFIKESDLRDDSIKDNSPPSLDSKIFLSDDDHKTFQENIYSNDRFDSKHSLTNHSKEIDQLLYQKFVPKLNGETLFSSDLLVKATDDVLPYKPRQKDHSQSFSHVPLRPNGEVGPKLSNSMGDVRSRVRQSFQNSFSAISEKGNRAFNILRQQEKAAKQLSLTLSCLFLCWTPYFVQVILYSICPQTANEKFLAVSVYLGYFHSLCNPILFVIFNLKFQRAFKKAMCCFPRDDKRKPYIPKQPSAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CNF4</accession>
    <name>A0A9Q1CNF4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_06675</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CNF4"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8047629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="104" mass="11960" checksum="2EAE9A9418A38569" modified="2023-09-13" version="1">MEMHQSSAFLLKSPIGQTYSIVHAFYVLMHLMGESIALPRRIILFICGEAVETDFSLNLYLRLCTTRCSSFNLSLQYSTCLTLQNQEDVIIFGERLFNENMDLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CNG2</accession>
    <name>A0A9Q1CNG2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Sterile alpha and TIR motif-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_07009</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CNG2"/>
    <dbReference type="OrthoDB" id="6158441at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052281">
        <property type="entry name" value="GAREM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14454">
        <property type="entry name" value="GRB2-ASSOCIATED AND REGULATOR OF MAPK PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14454:SF11">
        <property type="entry name" value="SERRANO, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAM" evidence="2">
        <location>
            <begin position="381"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="335"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="452"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="335"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="347"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="452"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8047885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="489" mass="55969" checksum="B1405295673B5F3C" modified="2023-09-13" version="1">MDRQVPLVPAKRSLTKGQFKTTDYRDEYSSAPLTGESHIYEDLRHALAGTTPFQRVCFQLNLDHTDADSKRDTAVYRLVSSFKLPFIAKVKRKFCGLGSDVVRLQKDDVLLFHFIGRIRRVLTIDDSGRKVMLPIQAKQVYDILPSVQQYDDKVFQGTKAVIEARPLPNRLRIVEAHCSMFPEESQEVGDVLEISHIERRGVLPPKTGVNYRRIRTPSDKRVYLLKEYIDDIVVIATYFNSQQVLAIPVTCQIDVEKETNLTTETLLHGLLPAINTDDATDQDRPLPNIPPGKDMKELLDKKEKEIQTLLQENKKNYEKLKEELVHLKDEYSQQNFSQDGEYSKGTNEQDIKQENEETDKTVVGGSAIEEKLSRQVKLLSASVEEVGSFLKDLRLDSYVPRFQEELVDGELLVRLDEDILKEDIGMKKIQARRLMLEVKKRLRNDEESDRVFEAVEHQDGTREVRGSIEKGPSIQENEGPGSRASTKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CQ75</accession>
    <name>A0A9Q1CQ75_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">LINE-1 retrotransposable element ORF2 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_00840</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CQ75"/>
    <dbReference type="OrthoDB" id="8961218at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09076">
        <property type="entry name" value="L1-EN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8048499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="214" mass="24884" checksum="CFE4375C9743911C" modified="2023-09-13" version="1">MWCTLRAEWGYELYFSGDRTNARGAAILLNNNFPFKMKRVDYNADCNYVAISVEIGRQDFTILSIYGPNRDSPNFYHHLGDILGELNDTFVILGGDWNCVLDSSLDCDGYLHDNNPIARSAILNMLNDYDLSDFYRVIHANRKVYTWRKLNPLKQARLDYFLVSKELLSFLDFVDTGLGYRTDHSLVQIKFVLNNQSPGRSYWKFNTSLLRDVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CRG0</accession>
    <name>A0A9Q1CRG0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Hillarin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_02537</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CRG0"/>
    <dbReference type="OrthoDB" id="6129702at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056564">
        <property type="entry name" value="Ig-like_KY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053041">
        <property type="entry name" value="Transglut-like_Superfamily_Mod"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47020">
        <property type="entry name" value="HILLARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47020:SF1">
        <property type="entry name" value="HILLARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23265">
        <property type="entry name" value="Ig-like_KY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KY-like immunoglobulin-like" evidence="2">
        <location>
            <begin position="442"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="101"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8049690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="913" mass="104661" checksum="38C89FC7F7B67634" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CSE8</accession>
    <name>A0A9Q1CSE8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_03306</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CSE8"/>
    <dbReference type="OrthoDB" id="5376140at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33480">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33480:SF1">
        <property type="entry name" value="TYR RECOMBINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8050200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="280" mass="31694" checksum="73081C094FC73847" modified="2023-09-13" version="1">MQRGTQVFVTTEVQKLPLQPTPKQHPNQTTTNHPKSKSHPPTNWPQRPPRPKAKAHAQRLPYARGQHHARRQRHAQRHTTPNGNTNAQRQQHVKSTGQIQVHLAQGCPGGSCGKGNKRVFFLLLRFRNCHCSPLQNNTLAKQPQSIPNIVPPVNHTPKGYHTPKDDTTPEGNKTPWAFNTTLNGARFLSGYNEETQEHARYSNALRLGFAVKDLAIAVRARYIKEGSRGEAAEVDEFLKLKDSEWRAKITSSVLRQRGEQSWKKPKILPLTSDCVKFNNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H1L3</accession>
    <name>A0A9Q1H1L3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Insulin-like growth factor-binding protein complex acid labile subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_25038</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H1L3"/>
    <dbReference type="OrthoDB" id="676979at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001164">
        <property type="entry name" value="GH01279p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050541">
        <property type="entry name" value="LRR_TM_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369">
        <property type="entry name" value="ANTIGEN BSP, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369:SF210">
        <property type="entry name" value="CHAOPTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="379"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8031737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="438" mass="49519" checksum="011F1194C2C8026C" modified="2023-09-13" version="1">MVLNVQVTWTGPTQPLQVNKTQTTPMCSDICDYYAYFKRAKCDKRNLYQVPQPSGCEDAVILEMHENHITHVDQEILSGYRKVNTLDISSNQIFNLQPGHFTNMSQLKNLLVSHNHLAEIPNGAFSGTESHLQRVYLNGNRISEINEGAFLGLRQVINMYLSYNLLKSLPSTIFHELTKLQYLSLSRNKLNWIEPSTFHGLENLQQLFLDSNKLTSLPEELFTGLNSLRTVNLSHNNLASIPPPEYLGLGPLQVLNIEANNMTKSHSLLPYLDIAQRLYIEENHFIYDCDFVKVCLWYFNQSETAPSQSELRIPLQCEEYVTEEDAPRRCESTSTESYPTENVSMSSRATSLTTLNVPGNKIPGLDTEFTSCSSKIPGMLFYCAVIGTLVLILLILKPVCKVLLKIKLCLKGHMHWTVTRTVKARGAAISGDLREDTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H3D5</accession>
    <name>A0A9Q1H3D5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_24665</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H3D5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040123813" description="Ig-like domain-containing protein" evidence="1">
        <location>
            <begin position="30"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="48"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8031468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="267" mass="30049" checksum="184F50B6DBBB1D59" modified="2023-09-13" version="1">MEERKDLDMSRLTVLFLLISFYCITCFKAENPCQGFYGATSHVHGVIGENISLTCHVSANCPRGYWKYDDNINPTWLVPKQYSGDTYFFNTSQGLTTLHIVNINDNVEGFYTCLCDYHNRSGIAHACYDLSVHVVQFPISVQINGEKHVFDICSKRSKAEMVDVEVNDNITIKCDDEAERRTNCLNMTKHISFIVKPSYHLCKFSCRPKGNSAKRELKWISLNVKNQNNSTTEVTSITVTSTTAAGKLVFTYNDNGCTSTSVLGSVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H3K1</accession>
    <name>A0A9Q1H3K1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Zinc finger protein 26</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">HOLleu_27894</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">Nanhai2018</strain>
            <tissue evidence="20">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TMEM255 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8031230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H3K1"/>
    <dbReference type="OrthoDB" id="427030at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002737">
        <property type="entry name" value="AGAP008430-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001485">
        <property type="entry name" value="Krueppel-related zinc finger protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000870">
        <property type="entry name" value="zinc finger protein 197 isoform X1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000264">
        <property type="entry name" value="Zinc finger protein 236"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000733">
        <property type="entry name" value="Zinc finger protein 236 variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001437">
        <property type="entry name" value="Zinc finger protein 594"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050826">
        <property type="entry name" value="Krueppel_C2H2_ZnFinger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028014">
        <property type="entry name" value="TMEM255"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24377">
        <property type="entry name" value="IP01015P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14967">
        <property type="entry name" value="FAM70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
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    <keyword evidence="12" id="KW-0238">DNA-binding</keyword>
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    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
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    <keyword evidence="11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 16" id="KW-0863">Zinc-finger</keyword>
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            <begin position="32"/>
            <end position="55"/>
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    <feature type="transmembrane region" description="Helical" evidence="18">
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            <end position="86"/>
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    <feature type="transmembrane region" description="Helical" evidence="18">
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            <end position="114"/>
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    <feature type="transmembrane region" description="Helical" evidence="18">
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            <begin position="200"/>
            <end position="224"/>
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            <end position="544"/>
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        </location>
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    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
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            <end position="698"/>
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    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="699"/>
            <end position="726"/>
        </location>
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            <end position="754"/>
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            <begin position="755"/>
            <end position="782"/>
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    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
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            <end position="810"/>
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        <location>
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            <end position="838"/>
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        <location>
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            <end position="866"/>
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        <location>
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            <begin position="254"/>
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            <begin position="355"/>
            <end position="367"/>
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            <begin position="368"/>
            <end position="377"/>
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        <location>
            <begin position="600"/>
            <end position="612"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="618"/>
            <end position="634"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
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    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
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    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAJ8031230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1143" mass="128008" checksum="864BD8D772C99A9E" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H8M4</accession>
    <name>A0A9Q1H8M4_HOLLE</name>
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        <recommendedName>
            <fullName evidence="6">Carboxylic ester hydrolase</fullName>
            <ecNumber evidence="6">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_20312</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the 'GDXG' lipolytic enzyme family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the type-B carboxylesterase/lipase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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    <dbReference type="EC" id="3.1.1.-" evidence="6"/>
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    </dbReference>
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        <property type="entry name" value="AB_hydrolase_fold"/>
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        <property type="entry name" value="Carboxylesterase_B_AS"/>
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        <property type="entry name" value="Carboxylesterase_B_CS"/>
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    </dbReference>
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        <property type="entry name" value="CARBOXYLIC ESTER HYDROLASE"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="CHOLNESTRASE"/>
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        <property type="entry name" value="CARBOXYLESTERASE_B_1"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0719">Serine esterase</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
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    </feature>
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            <begin position="33"/>
            <end position="561"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8036361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="615" mass="69773" checksum="873DD0111736D218" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H959</accession>
    <name>A0A9Q1H959_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SCAN box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_17561</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8036901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H959"/>
    <dbReference type="OrthoDB" id="10063366at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF13">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCAN box" evidence="2">
        <location>
            <begin position="175"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="269"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="269"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="284"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8036901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="428" mass="49416" checksum="F9481B606C8D8359" modified="2023-09-13" version="1">MRKDEIKRYVLEYLVDESILSSTVLETEMEREREREEREREERERERKMQKEKEEREMQMQREEKAREHEFRLKQLELGVIKGSDPKTGLDTGVFDVSKHVKFVPKFQEDNVEKFFNHFEKLGEQLKWPRDKWSILIQSNFTGKAQEVYSALSIEDSMDYDKVKRAILQAYELVPEAYRQKFRKYRKADTQTYVEFAYQKERHFDRWCASKKVSTFDTLRQLMLVEEFKRCVNDDIKTHLEENKADKLSEVANLADQYALTHKFGKVGQTQNKGQFNRPNKKYGNSSGTTGNSGSQSGSISPTNSGKKQPDSSRQKNNQVSSNKAAFKDISCGFCHKKGHTMANCFTLAKLKETETASNALANVGNSSEVVVTLRPNSNDKIKEEYLPFVSEGFISLDGNSAHPPVKIKILRDTGATQSLCSVVFSSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H9Q6</accession>
    <name>A0A9Q1H9Q6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Selenoprotein F</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_15567</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H9Q6"/>
    <dbReference type="OrthoDB" id="1910009at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039992">
        <property type="entry name" value="Sep15_SelM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13077">
        <property type="entry name" value="SELENOPROTEIN F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13077:SF6">
        <property type="entry name" value="SELENOPROTEIN F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040177387" evidence="1">
        <location>
            <begin position="28"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8038219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="90" mass="9895" checksum="9F47A973B9BC6F7E" modified="2023-09-13" version="1">MLPDLMGLVTMVTSVWIIMLHVSDTFAQLEVLPAGVCVERGFSSNLLCSSCDKLTEFGLTTLVEDCQRCCLKDADTERKTYAQAVLEVCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H9T6</accession>
    <name>A0A9Q1H9T6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Neurogenic locus notch-like protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_18631</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10006967at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019904">
        <property type="term" value="F:protein domain specific binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048646">
        <property type="term" value="P:anatomical structure formation involved in morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009952">
        <property type="term" value="P:anterior/posterior pattern specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030097">
        <property type="term" value="P:hemopoiesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051241">
        <property type="term" value="P:negative regulation of multicellular organismal process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051240">
        <property type="term" value="P:positive regulation of multicellular organismal process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035282">
        <property type="term" value="P:segmentation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048863">
        <property type="term" value="P:stem cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003008">
        <property type="term" value="P:system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000056">
        <property type="entry name" value="C-type LECtin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000424">
        <property type="entry name" value="Delta and Notch-like epidermal growth factor-related receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000012">
        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000004">
        <property type="entry name" value="Neurogenic locus notch 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000143">
        <property type="entry name" value="Protein crumbs 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000122">
        <property type="entry name" value="Protein crumbs homolog 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000472">
        <property type="entry name" value="Uncharacterized protein, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000006">
        <property type="entry name" value="Versican core protein-like isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="41"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12916">
        <property type="entry name" value="CYTOCHROME C OXIDASE POLYPEPTIDE VIC-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12916:SF4">
        <property type="entry name" value="UNINFLATABLE, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="50"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="23"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="49"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="47"/>
    </dbReference>
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        <property type="match status" value="4"/>
    </dbReference>
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    <keyword evidence="1 6" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
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            <end position="53"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="8">
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    </feature>
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        </location>
    </feature>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
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</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HA29</accession>
    <name>A0A9Q1HA29_HOLLE</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="1" type="ORF">HOLleu_18988</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="KAJ8038023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HA29"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8038023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="119" mass="13947" checksum="F0A656FB1CD60145" modified="2023-09-13" version="1">MQAKSATSKVHGLKWRGRKVSFCTTVTVTYYFAGSNVGKRKNLARYVLLLESFLSQSITQSANRGFSNGNRENFCKILTSIFLQNHMELEYFTVFYMKPVEYLQFCIRYRDSLHICRDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HBS5</accession>
    <name>A0A9Q1HBS5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Endonuclease/reverse transcript</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_14512</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8040274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="199" mass="23227" checksum="999FFB9820496398" modified="2023-09-13" version="1">MLMIVVCSRMTSHNLGLWAKTWGMRFQPSKCNIITFARKKPDVKLAAYKGLLRPVLEYACCVWDPHQSYLQDKLESIQRRSARFISSEFSREPGSMTVILKDLDLPTLAERRKENRLILFSKGFFGKANIPMDRLRHPTRTTRGMHNLHFCQLYSRSNCYKFSFFPKTLKDWNNLPADLIDGLDGHLDPVHYLTNFIRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HCW0</accession>
    <name>A0A9Q1HCW0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ras-related protein Rab-21</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_12298</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HCW0"/>
    <dbReference type="OrthoDB" id="28034at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001329">
        <property type="entry name" value="Small GTP-binding protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47978">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8041474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="223" mass="25543" checksum="5E4A7EB506AC9B9C" modified="2023-09-13" version="1">MQYPDSKPVQFKLILLGESGVGKTSLFSRYQAKEFPRNGHSATTVSCHCSRDFPVGARSRPVEVTLWDTANMERLHSLTDNYFREAEGAILVYDVADHMSLHAIPDWRMNCLKISPSAQLFIVGNKIDLQPRQFSMERTTVEHADLMAREFSEIEAIYRVSAKEDLKVEEMFHDIAQRMWEKYESKKLANGDTLTSEVSTIRLGQQGDDRVAPPRGKRRFQCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HDH6</accession>
    <name>A0A9Q1HDH6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_12078</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HDH6"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8041293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="138" mass="15481" checksum="047ECB6EE6CEAB46" modified="2023-09-13" version="1">MTYARPSSSQPLVKTSHHRQYFCDVGRLVQFCLGDTTGNICCRTVPTDALQRKMVQFVGHVMCPPVLSINDIPACPTSSLPNLHPTSPLPLAYILFQILFSIGSTSFWTVSVEPRYSMLVLNIQCYEELCKVGDRREV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HE55</accession>
    <name>A0A9Q1HE55_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">15-hydroxyprostaglandin dehydrogenase [NAD(+)]</fullName>
            <ecNumber evidence="3">1.1.1.141</ecNumber>
            <ecNumber evidence="4">1.1.1.232</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Eicosanoid/docosanoid dehydrogenase [NAD(+)]</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Prostaglandin dehydrogenase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">HOLleu_09816</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="22">Nanhai2018</strain>
            <tissue evidence="22">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the NAD-dependent dehydrogenation (oxidation) of a broad array of hydroxylated polyunsaturated fatty acids (mainly eicosanoids and docosanoids, including prostaglandins, lipoxins and resolvins), yielding their corresponding keto (oxo) metabolites. Decreases the levels of the pro-proliferative prostaglandins such as prostaglandin E2 (whose activity is increased in cancer because of an increase in the expression of cyclooxygenase 2) and generates oxo-fatty acid products that can profoundly influence cell function by abrogating pro-inflammatory cytokine expression. Converts resolvins E1, D1 and D2 to their oxo products, which represents a mode of resolvin inactivation. Resolvin E1 plays important roles during the resolution phase of acute inflammation, while resolvins D1 and D2 have a unique role in obesity-induced adipose inflammation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>(11R)-hydroxy-(5Z,8Z,12E,14Z)-eicosatetraenoate + NAD(+) = 11-oxo-(5Z,8Z,12E,14Z)-eicosatetraenoate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48640"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:78836"/>
            <dbReference type="ChEBI" id="CHEBI:90697"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:48641"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>(15S)-hydroxy-(5Z,8Z,11Z,13E)-eicosatetraenoate + NAD(+) = 15-oxo-(5Z,8Z,11Z,13E)-eicosatetraenoate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23260"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57409"/>
            <dbReference type="ChEBI" id="CHEBI:57410"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.232"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:23261"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>14-hydroxy-(4Z,7Z,10Z,12E,16Z,19Z)-docosahexaenoate + NAD(+) = 14-oxo-(4Z,7Z,10Z,12E,16Z,19Z)-docosahexaenoate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48952"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:90866"/>
            <dbReference type="ChEBI" id="CHEBI:90867"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:48953"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>15-oxo-(5S,6R)-dihydroxy-(7E,9E,11Z)-eicosatrienoate + NADH + H(+) = (5S,6R,15S)-trihydroxy-(7E,9E,11Z)-eicosatrienoate + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:41596"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:78325"/>
            <dbReference type="ChEBI" id="CHEBI:78329"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:41597"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>lipoxin A4 + NAD(+) = 15-oxo-(5S,6R)-dihydroxy-(7E,9E,11Z,13E)-eicosatetraenoate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41572"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:67026"/>
            <dbReference type="ChEBI" id="CHEBI:78311"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:41573"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>prostaglandin A1 + NAD(+) = 15-oxo-prostaglandin A1 + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41263"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57398"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:85072"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:41264"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>prostaglandin E1 + NAD(+) = 15-oxoprostaglandin E1 + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16477"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57397"/>
            <dbReference type="ChEBI" id="CHEBI:57401"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:16478"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>prostaglandin E2 + NAD(+) = 15-oxoprostaglandin E2 + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11876"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57400"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:606564"/>
            <dbReference type="EC" id="1.1.1.141"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:11877"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>resolvin D1 + NAD(+) = 17-oxoresolvin D1 + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50128"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132079"/>
            <dbReference type="ChEBI" id="CHEBI:132081"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:50129"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>resolvin D1 + NAD(+) = 8-oxoresolvin D1 + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50124"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132079"/>
            <dbReference type="ChEBI" id="CHEBI:132080"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:50125"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>resolvin D2 + NAD(+) = 16-oxoresolvin D2 + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53588"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:133367"/>
            <dbReference type="ChEBI" id="CHEBI:137498"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:53589"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>resolvin D2 + NAD(+) = 7-oxoresolvin D2 + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53584"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:133367"/>
            <dbReference type="ChEBI" id="CHEBI:137497"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:53585"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>resolvin E1 + NAD(+) = 18-oxo-resolvin E1 + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:49244"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:91000"/>
            <dbReference type="ChEBI" id="CHEBI:91001"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:49245"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.141" evidence="3"/>
    <dbReference type="EC" id="1.1.1.232" evidence="4"/>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8042929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HE55"/>
    <dbReference type="OrthoDB" id="417891at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047034">
        <property type="term" value="F:15-hydroxyicosatetraenoate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016404">
        <property type="term" value="F:15-hydroxyprostaglandin dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44229">
        <property type="entry name" value="15-HYDROXYPROSTAGLANDIN DEHYDROGENASE [NAD(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44229:SF4">
        <property type="entry name" value="15-HYDROXYPROSTAGLANDIN DEHYDROGENASE [NAD(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00039060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00040276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00041812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00042026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00045705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00049151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00049188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KAJ8042929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="193" mass="21164" checksum="B9A6B5432481ACEF" modified="2023-09-13" version="1">MKIEGTVAIVTGAAQGIGKAVTDRLLKHGARMLTRDGKAAEEEFTQKYGPDRVGFIWCDVAYLETLTDAFDETYKKHKRLDIVVNNAGIQDESQWGTLVMVNYEGDTAFTNGGMRVADVCPGYVDTALGNSGNLLLPPDNQPKPVPMESVVDAYIQIVEDDSMNGVVITATPTGNNEYHFKSNEEIDKELNLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HE80</accession>
    <name>A0A9Q1HE80_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_09935</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HE80"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8043019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="52" mass="6048" checksum="F163501BFB8E52FF" modified="2023-09-13" version="1">MHYSKRKNLIVFGGGQRSFGLTRGQTLKTLFTRYLKVGSLDSFYTWCVDALW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HF71</accession>
    <name>A0A9Q1HF71_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">C-factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_10900</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HF71"/>
    <dbReference type="OrthoDB" id="5296at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05325">
        <property type="entry name" value="carb_red_sniffer_like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051468">
        <property type="entry name" value="Fungal_SecMetab_SDRs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43544:SF7">
        <property type="entry name" value="NADB-LER2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43544">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE/REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8043695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="250" mass="26832" checksum="0750CB3F9C68C0C0" modified="2023-09-13" version="1">MSSILITGANRGIGLEFVRQLASNKEGKWKYIFAACRNPDKAEDYSVTLFCHFFYFLMFSDVTSDATIETAVKQVSSVVGDNGLNVVINNAGIIGGRERNSLDKLSRKGLLEVFDVNAAGALMVVQKFLPVLKQGAAASKTSGFSFYRGGIYNITSGVGSIADNTSGGTYAYRASKVALNMMTKSLSVDLKADNILAIALVPGWVQTDMGGSKAKLTVEDCVSMLLKVMDNAGEKENGEAVNYRGEIFPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HFM0</accession>
    <name>A0A9Q1HFM0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PID domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_10315</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HFM0"/>
    <dbReference type="OrthoDB" id="10038482at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051133">
        <property type="entry name" value="Adapter_Engulfment-Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11232">
        <property type="entry name" value="PHOSPHOTYROSINE INTERACTION DOMAIN-CONTAINING FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11232:SF74">
        <property type="entry name" value="PTB DOMAIN-CONTAINING ADAPTER PROTEIN CED-6-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00462">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PID" evidence="2">
        <location>
            <begin position="18"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="185"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="185"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8043291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="303" mass="35110" checksum="B1E4635F2FD67DBE" modified="2023-09-13" version="1">MAKILRKKKKSIKVEMNEELPFFQVLYMGRMETEARLDRDCVRNIVQQLVDKTRNEPLKPVRITITTRGLWVQEGPQFNPPGRETFIPIHNISYGAADQKYTKVFTFVTNFQEGGEAQKKGPTPFYSYVFRCETQAMARGMVVYLLRAFKAAYVTWHKDIKRIEFQKKLTDGPAGLREVDRACSDSNVRSEEGSDQHTVDSGCSTDDEVEPDGDTSKMVTLSNYLERWVRQQGTKRMNKATDTDELNYILNKEERDASFRTRADKYSRPDLLNCDVDLQREMSDPDVQNVFHRMMKMNITGTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HFZ9</accession>
    <name>A0A9Q1HFZ9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_07985</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HFZ9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="27"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8045059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="181" mass="20327" checksum="A1A680A430A47E68" modified="2023-09-13" version="1">MRETSPISTSTEQTTSDSKDDDNNTTAFILIATGVVMMIVILFSFVVFIVKRRKLGNLEIWNTNEQNMIGNDCQDPKRYTSVTVRPYLEYCAIMPPPLKDDTTRAEQEKTSNRTDLMEDQQDRQEYLMNGGAVSNSPIGISSGGCSTVESRPKHIKIKPYLEYCAIVPPPHKNPNLYSGDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HGG0</accession>
    <name>A0A9Q1HGG0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Presenilin</fullName>
            <ecNumber evidence="8">3.4.23.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_07879</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Probable subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endoplasmic reticulum membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Golgi apparatus membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="8">The PAL motif is required for normal active site conformation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the peptidase A22A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.23.-" evidence="8"/>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HGG0"/>
    <dbReference type="OrthoDB" id="20287at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070765">
        <property type="term" value="C:gamma-secretase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042500">
        <property type="term" value="F:aspartic endopeptidase activity, intramembrane cleaving"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034205">
        <property type="term" value="P:amyloid-beta formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055074">
        <property type="term" value="P:calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006509">
        <property type="term" value="P:membrane protein ectodomain proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.100:FF:000001">
        <property type="entry name" value="Presenilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.100">
        <property type="entry name" value="Presenilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001108">
        <property type="entry name" value="Peptidase_A22A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006639">
        <property type="entry name" value="Preselin/SPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042524">
        <property type="entry name" value="Presenilin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10202">
        <property type="entry name" value="PRESENILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10202:SF13">
        <property type="entry name" value="PRESENILIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01080">
        <property type="entry name" value="Presenilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01072">
        <property type="entry name" value="PRESENILIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00730">
        <property type="entry name" value="PSN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8" id="KW-0914">Notch signaling pathway</keyword>
    <keyword evidence="8" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="135"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="164"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="198"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="225"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="247"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="423"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="450"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="31"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="307"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="352"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="34"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="44"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="313"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="352"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8044970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="483" mass="54377" checksum="DA48B17E8FC947D8" modified="2023-09-13" version="1">MVRQSGANEYEDDECAVLLVDSEQEVKYFRNGSKMFSQSSTNRESSENDQRAEARRSEASRNAARSNTQDQEEEDEMLKYGAKHVIMLFVPVSLCMLLVVATISKVSYYQSSSVYLIYTPFHEETDQAGTKLWQALANAAILIAIVLLMTIFLVVLYKYKCYKFIHGWLIVASLMLLFVFSFIYLQELLVAFNVPMDYFTVCFIMWNFGVVGMVAVHWKGSLRVQQAYLIIISAMAALIFIKYLPEWTLWMILGAIALYDLFAVLTPHGPLRILVETAQDRNETLFPSLIYSSTMVWLINMADMDPESRNRKGSQNSSQQPLENNEQTAADEEAELDNGGFDSNFVQRQNEFTDSRSQSVNSEDARAAVSALRQGNPSPAARQTPPVDDEDDEERGVKLGLGDFIFYSVLVGKASYYGDWTTTLACFVAILVGLCLTLILLAIFKKALPALPISIAFGLTFYFTTANIVFPFTDAYASQQVFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HI96</accession>
    <name>A0A9Q1HI96_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Calcium-activated chloride channel regulator 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_06741</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HI96"/>
    <dbReference type="OrthoDB" id="6505736at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013642">
        <property type="entry name" value="CLCA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08434">
        <property type="entry name" value="CLCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040373652" evidence="3">
        <location>
            <begin position="21"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="770"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="domain" description="Calcium-activated chloride channel N-terminal" evidence="4">
        <location>
            <begin position="44"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="809"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="812"/>
            <end position="829"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8047684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="829" mass="92921" checksum="ECE7C6273412973D" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HIH4</accession>
    <name>A0A9Q1HIH4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Heparan sulfate glucosamine 3-O-sulfotransferase 3B1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_05781</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HIH4"/>
    <dbReference type="OrthoDB" id="411451at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008467">
        <property type="term" value="F:[heparan sulfate]-glucosamine 3-sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037359">
        <property type="entry name" value="NST/OST"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10605:SF65">
        <property type="entry name" value="GH20068P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10605">
        <property type="entry name" value="HEPARAN SULFATE SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sulfotransferase" evidence="6">
        <location>
            <begin position="49"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="active site" description="For sulfotransferase activity" evidence="3">
        <location>
            <position position="58"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="262"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="279"/>
            <end position="283"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="263"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR637359-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR637359-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR637359-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8046935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="320" mass="37211" checksum="9B5C19311A1EDDF2" modified="2023-09-13" version="1">MNENIIPRTFRDRVSRQGCYIYLGGAIEPKIRLAPSSIIETLKCERRLPSVFLIGVKKSGTTTLAKYLDLHKQIVLANEVRIPYNESTMEGTVHRYYLMMPLATPRQVVVANYPGYYWLNRVWLFRTLPYLPDVPKILVILRNPAERAISDYRHMELTGKGLTKEKKAHLFQGDVIKSNFEETVLLPNGSINTSLAFIQKGLYVTHLSEFARLIPKEHILILNGNEFAKDPLPILRKAESFLSLETFYQDNHFIYDKKKKFYCAKVSFRPDLTCMGSYKGRKHPTVSREVLKKLEAFYQPYNRRLKDEFGLSSADFEWIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HIV1</accession>
    <name>A0A9Q1HIV1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_06354</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HIV1"/>
    <dbReference type="OrthoDB" id="6361178at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8047370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="196" mass="21876" checksum="08F963682B6388E1" modified="2023-09-13" version="1">MPHIERKNPIVFGGGQRSPGVTRGQNLKTLLTQYLEMPHIERKNPIVFGGGQRSPGVTRGQNLKTLLTQYLEMRYGKRKNPIIFGGGQRSSGVTGGQTLKTSLTQYLKMPHIERKNPIVFGGGQRSPGVTRGQNLKTLLTQYLEMRYGKKKNPIIFGGGQRSFGVSGGQTLKTLLTRYLKKESSYSSQTWYIDAPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HJB8</accession>
    <name>A0A9Q1HJB8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein TASOR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_06060</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="5960959at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045814">
        <property type="term" value="P:negative regulation of gene expression, epigenetic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.228.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046432">
        <property type="entry name" value="TASOR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022188">
        <property type="entry name" value="TASOR_DUF3715"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16207">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16207:SF11">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12509">
        <property type="entry name" value="DUF3715"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TASOR pseudo-PARP" evidence="3">
        <location>
            <begin position="172"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="338"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="531"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="571"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="661"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="794"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="880"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="919"/>
            <end position="995"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1031"/>
            <end position="1058"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1165"/>
            <end position="1284"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="763"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="347"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="590"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="620"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="677"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="849"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="1033"/>
            <end position="1042"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1178"/>
            <end position="1208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1209"/>
            <end position="1219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1244"/>
            <end position="1255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8047146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1284" mass="142316" checksum="FB55539AB741CBA8" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HK08</accession>
    <name>A0A9Q1HK08_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_00995</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HK08"/>
    <dbReference type="OrthoDB" id="10458864at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8048615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="194" mass="21985" checksum="03C713B6450DBE62" modified="2023-09-13" version="1">MILLGYNYFKSEVDSHYQCRMSPPVNDPNEVTVESLRTGWTRNTVEENPHPPDAYYDTSVSHPPYRVDMINNGNNDAFGVFGCNVTKDGKMETIISITRMRSDADIVPSNGLFTQTVSTGDTNVPIRMMRTTEVPLESLRWRNNTFLWGDPHQGVDNFIIDEPVELYHAGIYECHIVGDRHSAKHGLNLLIVRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HLM0</accession>
    <name>A0A9Q1HLM0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CWF19-like protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_03984</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CWF19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HLM0"/>
    <dbReference type="OrthoDB" id="444325at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061632">
        <property type="term" value="F:RNA lariat debranching enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07380">
        <property type="entry name" value="MPP_CWF19_N"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CWF19-like cell cycle control factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040194">
        <property type="entry name" value="Cwf19-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006768">
        <property type="entry name" value="Cwf19-like_C_dom-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006767">
        <property type="entry name" value="Cwf19-like_C_dom-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12072">
        <property type="entry name" value="CWF19, CELL CYCLE CONTROL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12072:SF4">
        <property type="entry name" value="CWF19-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04677">
        <property type="entry name" value="CwfJ_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04676">
        <property type="entry name" value="CwfJ_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cwf19-like C-terminal" evidence="5">
        <location>
            <begin position="274"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="Cwf19-like protein C-terminal" evidence="4">
        <location>
            <begin position="399"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="251"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="258"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00041007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8050685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="490" mass="55548" checksum="D86D0AEA0605B289" modified="2023-09-13" version="1">MLLCVGDFFSDDPTDEEVWSKYQSGEENVPLPTYILGPNRPAHIKFYKDIKGCELCENITYLGKKGVFTGTSGLKIAYMSGIEGATQSDHEFTKTDVDALGLPLVSNSSFKGVDILLTSQWPRGMTKYAVPPEGMDVMTTGSPLISDLALVLRPRYHFAGREGLHYERLPYRNHKVLAESAKQVTRFVGLAKVGNAEKKKSLYAFNITPMCNMTPDDLTKQPVDVTECPYSYSERPVEKQQVDMSNQYFYSTNQQQDRGQKRSRDQSSQDRRPKRPSQPAGACWFCLSSPEVEKHMVVSIGTHTYVALAKGGLVPDHVLILPIGHHQSTVELSEEILKELEQYKSSLRKYFKSIGKSCVIFERNFKTSHLQLQVVPVESVVTEDILEEFQRLADEHNIELNQIPSLSDLKQIISVGAPYFYVELDSGEKLLHRVKKFFPLQFGREVMAGRALLDLPDRVDWKNCKTSTEEEKQMAEDFRSKFAKFDPNTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HLZ9</accession>
    <name>A0A9Q1HLZ9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_03775</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HLZ9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8050536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="210" mass="23714" checksum="AF233AB719021FCF" modified="2023-09-13" version="1">MISIKVEPADTPEWEEDTHVRTINPEKQTVVSAKDTGDSIVVKIEQDSCLEEGKEDVVEKEETKEQCQEQGDSVVVKIEEDSCLEEGKEDGVEKEETREQFQEQGRSTGNDDRILQVTVRMDADGSLVEEEEYINAALVRKTNEDLGSNCVYCIHLNFGHELWNDGRILQVTVRMDADGSLVEEEEYINAALVRNKHADLGSKGTYYINT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0Y9Q4</accession>
    <name>A0A9Q0Y9Q4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">LINE-1 reverse transcriptase-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_45181</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010002035">
        <property type="protein sequence ID" value="KAJ8017426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0Y9Q4"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8017426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="298" mass="34473" checksum="6ABB0961ACD32770" modified="2023-09-13" version="1">MYTWHSNIDRAIHCRLDFFVVSRHLRTSVLNSSIISLIGSDHSGVSLELQIGVARGKGTWKLNTALLDDPIYIETIKSAIHSTVNSCRDKDPSFVWEACKLSIRSASIAYSKYLAIQRDCYERFLIDRISHLEQLQANSPSEQCKDQLTDARSALNIIYDFKLKGIIIRSRARWTEDGEKSTQYFLNLEKRNRTLNTINELVLENNEVIKNPTDILQELEQFYKKLYTKRSDVMSEIFFMNTTLNHFKLSSEQQRSCEGKISLEDCYISLHSMLLKSHLVQMVCQLNFIRFSGRKLGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YC28</accession>
    <name>A0A9Q0YC28_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_44809</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010001267">
        <property type="protein sequence ID" value="KAJ8017639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YC28"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8017639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="57" mass="6483" checksum="718DAFB6E9C8D16A" modified="2023-09-13" version="1">MRLQKLHDVIVQPEGENGDVPVDRAKNAEAFAELVQFLDDRSLSLVMRDAKDNGRKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YC38</accession>
    <name>A0A9Q0YC38_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Reverse transcriptase RNase H-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_43163</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000221">
        <property type="protein sequence ID" value="KAJ8018701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YC38"/>
    <dbReference type="OrthoDB" id="5989411at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09274">
        <property type="entry name" value="RNase_HI_RT_Ty3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041373">
        <property type="entry name" value="RT_RNaseH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17917">
        <property type="entry name" value="RT_RNaseH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Reverse transcriptase RNase H-like" evidence="7">
        <location>
            <begin position="24"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF17917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8018701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="245" mass="28211" checksum="A64B5097AAF13D4A" modified="2023-09-13" version="1">MPRRTGWGQLFLIVFPKAKNRSLFASRKLTKAEQNYSQLEREALAIVYGIRTFHQYLCGKKFTLFTDHKPLKYLLGPNKGIPVLAASRLQRWAILLSGYEFDIKYRTSKQNANVDCLSRLPIGDVNASKTTKEATNMNKVQIKSLPVSAKEIGNATNNDKILARVKHFVLYGWPNEQTLSPELIPYYRKKDELSVESNCLLWGLRVIIPYKYQDYVLKELHTSHPGMAKMKSLARMQVLWPYQGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YCK9</accession>
    <name>A0A9Q0YCK9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Fibrinogen-like protein A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_41509</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8019780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YCK9"/>
    <dbReference type="OrthoDB" id="6275059at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005577">
        <property type="term" value="C:fibrinogen complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005201">
        <property type="term" value="F:extracellular matrix structural constituent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034116">
        <property type="term" value="P:positive regulation of heterotypic cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037579">
        <property type="entry name" value="FIB_ANG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040941">
        <property type="entry name" value="GGGWT_bact"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47221">
        <property type="entry name" value="FIBRINOGEN ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47221:SF5">
        <property type="entry name" value="FIBRINOGEN C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00186">
        <property type="entry name" value="FBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040220709" evidence="5">
        <location>
            <begin position="30"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="6">
        <location>
            <begin position="42"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="6">
        <location>
            <begin position="201"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8019780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="265" mass="30367" checksum="37E380D11726C539" modified="2023-09-13" version="1">MSLLHRLFQYYFVLCTTCIFWNSAQITQGDTTINQGSSYFFYQRPEYPKDCKDVLNECSSSISSGVYLIKPDGYSEPFEVFCNHDLDSGGWMVVQRRIDGSVSFNRNWEEYRKGFGFLSTEFWLGNEKLSYIINQEVYELRIDMMLSNGSSFYIKYNNFCITDEWGSFSLTSVGEFSGNESSVITLCPPNTVAQNSTCEPVQETQIYTDCYHAYQTGRRDDGVYTIRPSGWTGEPFKVPCNMSIDGGGWTVTIILYQTQWGGRCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YCR7</accession>
    <name>A0A9Q0YCR7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Chondroadherin-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_40008</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YCR7"/>
    <dbReference type="OrthoDB" id="6363818at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
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    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050541">
        <property type="entry name" value="LRR_TM_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369">
        <property type="entry name" value="ANTIGEN BSP, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369:SF210">
        <property type="entry name" value="CHAOPTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040184552" evidence="6">
        <location>
            <begin position="21"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="409"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="domain" description="LRRCT" evidence="7">
        <location>
            <begin position="286"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="337"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="473"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="518"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="573"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="618"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="337"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="522"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="535"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="594"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="618"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="654"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="687"/>
            <end position="706"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8020423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="723" mass="81406" checksum="BDB0FAD9FE359C82" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YCZ5</accession>
    <name>A0A9Q0YCZ5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Oxidation resistance protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_40102</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the OXR1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="A0A9Q0YCZ5"/>
    <dbReference type="OrthoDB" id="26679at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00118">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.350.10">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018392">
        <property type="entry name" value="LysM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036779">
        <property type="entry name" value="LysM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354:SF62">
        <property type="entry name" value="MUSTARD, ISOFORM V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354">
        <property type="entry name" value="NUCLEOLAR PROTEIN 7/ESTROGEN RECEPTOR COACTIVATOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01476">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00257">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54106">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51782">
        <property type="entry name" value="LYSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51886">
        <property type="entry name" value="TLDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="75"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="TLDc" evidence="7">
        <location>
            <begin position="861"/>
            <end position="1027"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="130"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="317"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="663"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="130"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="184"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="196"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="321"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="362"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="390"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="401"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="464"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="513"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="556"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="663"/>
            <end position="676"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00040604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8020497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1028" mass="115804" checksum="EB6381BC87C9B700" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YDC4</accession>
    <name>A0A9Q0YDC4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Leukocyte elastase inhibitor C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_40390</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the serpin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YDC4"/>
    <dbReference type="OrthoDB" id="671595at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00172">
        <property type="entry name" value="serpin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.39.10">
        <property type="entry name" value="Alpha-1-antitrypsin, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.497.10">
        <property type="entry name" value="Antithrombin, subunit I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023795">
        <property type="entry name" value="Serpin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023796">
        <property type="entry name" value="Serpin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000215">
        <property type="entry name" value="Serpin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036186">
        <property type="entry name" value="Serpin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042178">
        <property type="entry name" value="Serpin_sf_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042185">
        <property type="entry name" value="Serpin_sf_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461:SF211">
        <property type="entry name" value="GH10112P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461">
        <property type="entry name" value="SERINE PROTEASE INHIBITOR, SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00079">
        <property type="entry name" value="Serpin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00093">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56574">
        <property type="entry name" value="Serpins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00284">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serpin" evidence="3">
        <location>
            <begin position="22"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8020723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="405" mass="45920" checksum="3B421E5E8BE1B5A7" modified="2023-09-13" version="1">MGADVSTAEAESLTPVINEFAFHFFKSVGSSQDGNIVFSPAIIATGLALLHSGSKKETASQISRVVNFEQFEKSDLDTAMVALRAFFFEAGHTYQFFGASRIYSPKNIKISKKFRKSALDSYKTVVEGHSLEDPKSSAQEMNHWVSEAMAHTVTKDVVAENQLDHSDKLILLNALYFKGSFAVPFHRAMTKVGKFFMSETDNYNVAMMNVKNSFPYFHDEEMKCQVVEMPYAELHYRVILILPDERTGLSELESRLTVKRLQKWCRPREMEVVDIHVPRFDISQTMIANETLHKMGIELLFDPEKADLSNIGNHLCLSKFVHYARADVKEETAESEQPPGDVHGFVVSFKTFKADHPFLFLIQDKRKGAIAFMGRQTAPPKIDPEELKEIYKRAKSKLRAKATEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YDC8</accession>
    <name>A0A9Q0YDC8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">peptidyl-tRNA hydrolase</fullName>
            <ecNumber evidence="1">3.1.1.29</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_39765</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>an N-acyl-L-alpha-aminoacyl-tRNA + H2O = an N-acyl-L-amino acid + a tRNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54448"/>
            <dbReference type="Rhea" id="RHEA-COMP:10123"/>
            <dbReference type="Rhea" id="RHEA-COMP:13883"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:59874"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:138191"/>
            <dbReference type="EC" id="3.1.1.29"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.29" evidence="1"/>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YDC8"/>
    <dbReference type="OrthoDB" id="201213at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004045">
        <property type="term" value="F:peptidyl-tRNA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd02429">
        <property type="entry name" value="PTH2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1490.10">
        <property type="entry name" value="Bit1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023476">
        <property type="entry name" value="Pep_tRNA_hydro_II_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002833">
        <property type="entry name" value="PTH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042237">
        <property type="entry name" value="PTRHD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46194">
        <property type="entry name" value="PEPTIDYL-TRNA HYDROLASE PTRHD1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46194:SF1">
        <property type="entry name" value="PEPTIDYL-TRNA HYDROLASE PTRHD1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01981">
        <property type="entry name" value="PTH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102462">
        <property type="entry name" value="Peptidyl-tRNA hydrolase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00048707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8020230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="119" mass="13652" checksum="5FF32A1B2B4EB900" modified="2023-09-13" version="1">MASSMVQYVVVRGDLLHSLKWPTGAIIAQACHACTAVLHLYRDDENVVQYTSDLDNMHKVVLEAKNEGDLRTLSDKLSEGSIHHKLWIEQPEDYATCLAAKPYPKEEIQKYFKKFKLFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YE36</accession>
    <name>A0A9Q0YE36_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_40300</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YE36"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8020650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="63" mass="7240" checksum="6796820F3D723FB9" modified="2023-09-13" version="1">MGMIKYKLRSERSLSIGRFEASHIAHQAQHWFCSQGTPLLRGRFFSAHRGGFALIWESCETLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YFE3</accession>
    <name>A0A9Q0YFE3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Integrase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_42502</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000081">
        <property type="protein sequence ID" value="KAJ8019122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YFE3"/>
    <dbReference type="OrthoDB" id="10049357at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331:SF6">
        <property type="entry name" value="DOUBLECORTIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase zinc-binding" evidence="1">
        <location>
            <begin position="36"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF17921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8019122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="112" mass="12961" checksum="49C8B82A5B62F61B" modified="2023-09-13" version="1">MKKKIVYVGGRADNAHFTYERRHPVLLPKDHHISFLVVQHVHEQGHYGVATTIAKVRSRYWIVGATKLAKTVKFRCVTCRRFRQKTETQLIANLPLERMAPFTPPVLDKNTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YFF3</accession>
    <name>A0A9Q0YFF3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_44804</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010001260">
        <property type="protein sequence ID" value="KAJ8017641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YFF3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8017641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="177" mass="19460" checksum="07FD4A3601AAB747" modified="2023-09-13" version="1">MPHIEKKNPIVFGGGQRSPGVTGGQTLKTLLTQYLEMSHIKRKNPIVFGGGQRSPGVTRGQTLKTLLTQYLEMHYGMRKNPIIFGGGERSSGVTGGQTLKTSLTRYLKMPHTKRMKPIVFGGDRRSPGVTGGQTLKTLLTQYLEMRFGKRKNRIIFDGGERSSGVTGGQTLKILLTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YG45</accession>
    <name>A0A9Q0YG45_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ethanolamine kinase</fullName>
            <ecNumber evidence="5">2.7.1.82</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_38525</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="3">Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from ethanolamine: step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the choline/ethanolamine kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.82" evidence="5"/>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YG45"/>
    <dbReference type="OrthoDB" id="10267235at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004305">
        <property type="term" value="F:ethanolamine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006646">
        <property type="term" value="P:phosphatidylethanolamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05157">
        <property type="entry name" value="ETNK_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22603">
        <property type="entry name" value="CHOLINE/ETHANOALAMINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22603:SF66">
        <property type="entry name" value="ETHANOLAMINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01633">
        <property type="entry name" value="Choline_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="1" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="1" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="2" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00037883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8021354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="328" mass="38182" checksum="6C417803F60E72EB" modified="2023-09-13" version="1">MNGLDVHLDLFGSHYPYAKKVFTDGASNKLIGAYLGDNKRNMILVRIFGNKTELIINREQELKAFQVLYEAGFGPQLYATFKNGISYGFMPGSIVSTETVRSEKLYPLITREMARLHTLKPKDGFTFEPCLFVTLKKWLKILPKEFEDKEKQERYKRDAPGHETLEKEMNELEQLLTPLGSPVVFTHNDLRLANIIYDDKENKISFIDYEYASYNYQGFDIGNHFDDFACVEGVDNPDKELLLKWLREYLKYWHEFNNISDPVTDQEIEKLYVIANKFSLASHLLWATWGLIQAHNSTIDVDFLGFAITRMNEYFKNKDKFFALPMPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YHA6</accession>
    <name>A0A9Q0YHA6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Metalloendopeptidase</fullName>
            <ecNumber evidence="13">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HOLleu_38675</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Nanhai2018</strain>
            <tissue evidence="16">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Metalloprotease.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12 13">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="12 13">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="13"/>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YHA6"/>
    <dbReference type="OrthoDB" id="291007at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04280">
        <property type="entry name" value="ZnMc_astacin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000015">
        <property type="entry name" value="Meprin A subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034035">
        <property type="entry name" value="Astacin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001506">
        <property type="entry name" value="Peptidase_M12A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003582">
        <property type="entry name" value="ShKT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10127">
        <property type="entry name" value="DISCOIDIN, CUB, EGF, LAMININ , AND ZINC METALLOPROTEASE DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10127:SF780">
        <property type="entry name" value="METALLOENDOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01400">
        <property type="entry name" value="Astacin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01549">
        <property type="entry name" value="ShK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00480">
        <property type="entry name" value="ASTACIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00254">
        <property type="entry name" value="ShKT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51864">
        <property type="entry name" value="ASTACIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51670">
        <property type="entry name" value="SHKT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 12 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 12 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 12 13" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2 12 13" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0732">Signal</keyword>
    <keyword evidence="6 12 13" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040530607" description="Metalloendopeptidase" evidence="13">
        <location>
            <begin position="22"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M12A" evidence="15">
        <location>
            <begin position="106"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="ShKT" evidence="14">
        <location>
            <begin position="314"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="domain" description="ShKT" evidence="14">
        <location>
            <begin position="355"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="198"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="314"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="355"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ8021461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="389" mass="44634" checksum="3C73BC703CC0F53D" modified="2023-09-13" version="1">MKFLVSQLSVFIAVWIGFSSAKPLTRGTDNANLDVVDVQLIEETPSFEMNLKTPTAYELLTFGTDQEEGSMEYILQENQNIQSGVEHYLIEGDIKVDYNVSQVSPNAVRNRNVRWPGGVVPYFIDRSFDSGARQRILAAIERYHQTTCIRFVPWNGQRDYVHIVPRQGCFSAVGRRGGRQELSVGGACNFHRGTIMHELMHAVGFQHEQTRTDRDQFVTVYYQNIQPGLEYNFIRYNQDTIDHLQTRYDYYSIMHYPMYAFSRNGRPTIAPRQSGVSIGNRNDFSTTDILKINRYYECEDTTDNGGEETNPDDCEETHPNCSVWAQRGECSRNPAWMLPNCPVSCRQCRPSTPGCSDDNDFCSQWARNGECQRNPRYMLTSCRQSCNVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YIT0</accession>
    <name>A0A9Q0YIT0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_35658</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YIT0"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8023288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="105" mass="12335" checksum="95EDE418C8F82AB5" modified="2023-09-13" version="1">MPPLLNTLPTHMVYRVPPHGALGVPHDVPSAPHTVQRVPHMVPRVLLTDAINRRYLILPTYLCIFLLRILRIHHLHHRLLLHLRNETCWGLHTFHDVIYQMTSRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YJ25</accession>
    <name>A0A9Q0YJ25_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 17">Alkaline phosphatase</fullName>
            <ecNumber evidence="3 17">3.1.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">HOLleu_38338</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">Nanhai2018</strain>
            <tissue evidence="19">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a phosphate monoester + H2O = an alcohol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15017"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:67140"/>
            <dbReference type="EC" id="3.1.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="15">Binds 1 Mg(2+) ion.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="15">Binds 2 Zn(2+) ions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the alkaline phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.1" evidence="3 17"/>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YJ25"/>
    <dbReference type="OrthoDB" id="5818554at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004035">
        <property type="term" value="F:alkaline phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16012">
        <property type="entry name" value="ALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.720.10:FF:000008">
        <property type="entry name" value="Alkaline phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001952">
        <property type="entry name" value="Alkaline_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018299">
        <property type="entry name" value="Alkaline_phosphatase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11596">
        <property type="entry name" value="ALKALINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11596:SF5">
        <property type="entry name" value="ALKALINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00245">
        <property type="entry name" value="Alk_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00113">
        <property type="entry name" value="ALKPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00098">
        <property type="entry name" value="alkPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00123">
        <property type="entry name" value="ALKALINE_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8 17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10 15 17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="18" id="KW-0732">Signal</keyword>
    <keyword evidence="9 15 17" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040420339" description="Alkaline phosphatase" evidence="18">
        <location>
            <begin position="32"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphoserine intermediate" evidence="14">
        <location>
            <position position="135"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="358"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="363"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="367"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="404"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="405"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="489"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR601952-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR601952-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU003946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAJ8021207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="556" mass="61129" checksum="CB5F6423BCD0DFBE" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YJK5</accession>
    <name>A0A9Q0YJK5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Kazal-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_36309</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YJK5"/>
    <dbReference type="OrthoDB" id="88467at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00104">
        <property type="entry name" value="KAZAL_FS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.30">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036058">
        <property type="entry name" value="Kazal_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050653">
        <property type="entry name" value="Prot_Inhib_GrowthFact_Antg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10913:SF45">
        <property type="entry name" value="FOLLISTATIN, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10913">
        <property type="entry name" value="FOLLISTATIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00050">
        <property type="entry name" value="Kazal_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07648">
        <property type="entry name" value="Kazal_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00280">
        <property type="entry name" value="KAZAL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100895">
        <property type="entry name" value="Kazal-type serine protease inhibitors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51465">
        <property type="entry name" value="KAZAL_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0722">Serine protease inhibitor</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040265825" description="Kazal-like domain-containing protein" evidence="4">
        <location>
            <begin position="19"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="domain" description="Kazal-like" evidence="5">
        <location>
            <begin position="20"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="Kazal-like" evidence="5">
        <location>
            <begin position="221"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8023773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="719" mass="78971" checksum="330BE7F2D5C4579A" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YJN6</accession>
    <name>A0A9Q0YJN6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">GTPase IMAP family member 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_36359</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. AIG1/Toc34/Toc159-like paraseptin GTPase family. IAN subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YJN6"/>
    <dbReference type="OrthoDB" id="5985928at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000840">
        <property type="entry name" value="Immune-associated nucleotide-binding protein 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006703">
        <property type="entry name" value="G_AIG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045058">
        <property type="entry name" value="GIMA/IAN/Toc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903:SF184">
        <property type="entry name" value="GTP-BINDING PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903">
        <property type="entry name" value="GTPASE, IMAP FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04548">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51720">
        <property type="entry name" value="G_AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIG1-type G" evidence="5">
        <location>
            <begin position="53"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="309"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8023815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="402" mass="46050" checksum="9AFDD3DD37D15758" modified="2023-09-13" version="1">MDRDYITDENWEKFRNSSETLSLLASVGKQSFEMMQENYGLKQNRNYVHPSSTDDLRIVVIGKTGVGKSATANTILGEEVFEDSEGSVSETSSSRCVSRNLNDRTVSVIDTPGLQDTSRPQKEVLREIARVMKLFHEGVHVFIYVLNMASPRFTDEDKRSLAAIEQMKFGDNMKAHRMLVFTHADSTLADRRTLQAFCEEQQRKSPAITTFFEELDGNIVAVNNKYKFPAEKKRNQNVILGLADKIKHTNNNAVYTNDMFEKAAEEVRKLMKKFVDEGFNHRIMDGVDIAIAENPDIVDSDQKLVVEVRQKLHEEIKRQQDKIYELRKARAEEEKKRIEEEVKQNIKRELAEAEEQLKSTSEVLESDMIEEQAGKIGKSHLGVLKTLQEYMTSLDVWKGIGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YJS1</accession>
    <name>A0A9Q0YJS1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">DNA damage-binding protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_36301</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DDB1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YJS1"/>
    <dbReference type="OrthoDB" id="433457at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000070">
        <property type="entry name" value="DNA damage-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:002576">
        <property type="entry name" value="DNA damage-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.910:FF:000003">
        <property type="entry name" value="DNA damage-binding protein 1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018846">
        <property type="entry name" value="Beta-prop_RSE1/DDB1/CPSF1_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058543">
        <property type="entry name" value="Beta-prop_RSE1/DDB1/CPSF1_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050358">
        <property type="entry name" value="RSE1/DDB1/CFT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004871">
        <property type="entry name" value="RSE1/DDB1/CPSF1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10644">
        <property type="entry name" value="DNA REPAIR/RNA PROCESSING CPSF FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10433">
        <property type="entry name" value="Beta-prop_RSE1_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23726">
        <property type="entry name" value="Beta-prop_RSE1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03178">
        <property type="entry name" value="CPSF_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101908">
        <property type="entry name" value="Putative isomerase YbhE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RSE1/DDB1/CPSF1 first beta-propeller" evidence="5">
        <location>
            <begin position="15"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="domain" description="RSE1/DDB1/CPSF1 second beta-propeller" evidence="6">
        <location>
            <begin position="397"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="domain" description="RSE1/DDB1/CPSF1 C-terminal" evidence="4">
        <location>
            <begin position="791"/>
            <end position="1100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF10433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8023767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1141" mass="126082" checksum="09921B51A1EBBA74" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YKS8</accession>
    <name>A0A9Q0YKS8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Zinc finger MYM-type protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_38225</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8023130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YKS8"/>
    <dbReference type="OrthoDB" id="6608798at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45749">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45749:SF21">
        <property type="entry name" value="DUF4371 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="148"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8023130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="170" mass="19323" checksum="314849461E39D250" modified="2023-09-13" version="1">MYSVMMDETTYTSNLEQVTISVRFIFEGPIEEWFLDITAIADNTGEALARHLIARMESDSLSLNQALSQSYDGAGNMSGQYRGVQARVWEQYPHVTYVYCYAHNLNHLLVNICSQVPVARDFFALVEELYVIIEGSSKRHFFISAQKRHRPNETPRQLAGLSDSPGIVKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YLK6</accession>
    <name>A0A9Q0YLK6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_34555</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="3"/>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YLK6"/>
    <dbReference type="OrthoDB" id="5854685at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13189">
        <property type="entry name" value="FERM_C_PTPN4_PTPN3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14541">
        <property type="entry name" value="PTPc-N3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000002">
        <property type="entry name" value="Band 4.1-like protein 5 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000023">
        <property type="entry name" value="Tyrosine-protein phosphatase non-receptor type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000003">
        <property type="entry name" value="Tyrosine-protein phosphatase non-receptor type 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014847">
        <property type="entry name" value="FA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019747">
        <property type="entry name" value="FERM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018979">
        <property type="entry name" value="FERM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018980">
        <property type="entry name" value="FERM_PH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041783">
        <property type="entry name" value="PTPN3/4_FERM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45706">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45706:SF4">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08736">
        <property type="entry name" value="FA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09380">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00373">
        <property type="entry name" value="FERM_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09379">
        <property type="entry name" value="FERM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00935">
        <property type="entry name" value="BAND41"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01195">
        <property type="entry name" value="FA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01196">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00660">
        <property type="entry name" value="FERM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00661">
        <property type="entry name" value="FERM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FERM" evidence="11">
        <location>
            <begin position="28"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="12">
        <location>
            <begin position="469"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="9">
        <location>
            <begin position="603"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="10">
        <location>
            <begin position="779"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="377"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="377"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8024609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="877" mass="101028" checksum="895857D647EB4090" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YNG4</accession>
    <name>A0A9Q0YNG4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_35871</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YNG4"/>
    <dbReference type="OrthoDB" id="5986179at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1310.20">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8023427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="99" mass="11466" checksum="A44EC4D556FABDCD" modified="2023-09-13" version="1">MASELGARQVRMVYLITYRKADCNVCNSREDFASKILSAFRSSGIKVMHWACSRENHQDGGHHYHMSVKLDQGRRWLRVKQTLEAEHSMKVNFSSTHVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YRC8</accession>
    <name>A0A9Q0YRC8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Securin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_33862</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8026115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YRC8"/>
    <dbReference type="OrthoDB" id="10015025at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8026115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="234" mass="25866" checksum="C386147935D6D512" modified="2023-09-13" version="1">MASLIQPYVDKENATSMGGGVRKGRLQSFGQRHAVGKTFGSENVQTPRQVLGNISNDPNAVRSTAKSIGQKANIQQSIKKPALMKNPQGLSMRKSSSNTPGQPLLQKKKMTKIAPPAPIFKVPPSLLKDNLPPIEQMHIFEDKEIPDCIPAEHCLSAHLGKFLPIGRPPVRKMTLLNEPSPDFSNLTLENTVRPIFEDDAVQLPPMKDFDACLNLPPVETQDFMNINNIKLWDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YRE3</accession>
    <name>A0A9Q0YRE3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">tRNA-dihydrouridine(47) synthase [NAD(P)(+)]</fullName>
            <ecNumber evidence="2">1.3.1.89</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_35476</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>5,6-dihydrouridine(47) in tRNA + NAD(+) = uridine(47) in tRNA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53364"/>
            <dbReference type="Rhea" id="RHEA-COMP:13539"/>
            <dbReference type="Rhea" id="RHEA-COMP:13540"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.89"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="3">
            <dbReference type="Rhea" id="RHEA:53366"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>5,6-dihydrouridine(47) in tRNA + NADP(+) = uridine(47) in tRNA + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53360"/>
            <dbReference type="Rhea" id="RHEA-COMP:13539"/>
            <dbReference type="Rhea" id="RHEA-COMP:13540"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.89"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="6">
            <dbReference type="Rhea" id="RHEA:53362"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a 5,6-dihydrouridine in mRNA + NAD(+) = a uridine in mRNA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:69851"/>
            <dbReference type="Rhea" id="RHEA-COMP:14658"/>
            <dbReference type="Rhea" id="RHEA-COMP:17789"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="4">
            <dbReference type="Rhea" id="RHEA:69853"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 5,6-dihydrouridine in mRNA + NADP(+) = a uridine in mRNA + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:69855"/>
            <dbReference type="Rhea" id="RHEA-COMP:14658"/>
            <dbReference type="Rhea" id="RHEA-COMP:17789"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="5">
            <dbReference type="Rhea" id="RHEA:69857"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Dus family. Dus3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.89" evidence="2"/>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8025304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YRE3"/>
    <dbReference type="OrthoDB" id="259935at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102265">
        <property type="term" value="F:tRNA-dihydrouridine47 synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035587">
        <property type="entry name" value="DUS-like_FMN-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45846">
        <property type="entry name" value="TRNA-DIHYDROURIDINE(47) SYNTHASE [NAD(P)(+)]-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45846:SF1">
        <property type="entry name" value="TRNA-DIHYDROURIDINE(47) SYNTHASE [NAD(P)(+)]-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01207">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUS-like FMN-binding" evidence="7">
        <location>
            <begin position="44"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00048266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8025304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="148" mass="16941" checksum="51389C767A43CA61" modified="2023-09-13" version="1">MALLIEDLFDLLTVGFLDFIDRAMASCEVYVHTSYNLYFQRQKLGVGRNGDILSFEDANEHFSNSQSAGLMIARGALIKPWIFTEIKEQRHWDISASERLDILRDFTNYGLENWGSDTQVGVHIILGILHELWSREVSSDMEVGVHFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YS48</accession>
    <name>A0A9Q0YS48_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_31350</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YS48"/>
    <dbReference type="OrthoDB" id="419598at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8026505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="56" mass="6136" checksum="84F825C22B4250EA" modified="2023-09-13" version="1">MSCLGSSAVRNIVTIYSESVRVITKAMKKAGVSRFVALTSWCTSGRLITKSFFQCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YT85</accession>
    <name>A0A9Q0YT85_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Polycomb group RING finger protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_34173</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8026352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YT85"/>
    <dbReference type="OrthoDB" id="1305878at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035102">
        <property type="term" value="C:PRC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990841">
        <property type="term" value="F:promoter-specific chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17081">
        <property type="entry name" value="RAWUL_PCGF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16733">
        <property type="entry name" value="RING-HC_PCGF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000099">
        <property type="entry name" value="Polycomb group RING finger protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000122">
        <property type="entry name" value="polycomb group RING finger protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032443">
        <property type="entry name" value="RAWUL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10825:SF29">
        <property type="entry name" value="POLYCOMB GROUP RING FINGER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10825">
        <property type="entry name" value="RING FINGER DOMAIN-CONTAINING, POLYCOMB GROUP COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16207">
        <property type="entry name" value="RAWUL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="11">
        <location>
            <begin position="27"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00040075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8026352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="246" mass="28912" checksum="3C80C0D37A538B66" modified="2023-09-13" version="1">MYHFDDDDLDDEPLRINIRELNQHVVCILCAGYFIDATTIIECLHTFCKSCIVKYLQTSKHCPMCNIKIHETQPLLNLRPDRTMQDIIFKLIPGLFDSEEQRKKEFYKSRGLDKVQSKVQQKSFSGKLDPTGGHFYRNDEQLSLCLELMESNEKEEIQAESILAKKFIRCSVRTCVYHLQKLLIKKLHIPENMTVHVFCDGERLHEDEPMKLIWLTHWFEKAAPMVLHYQPVPREKTVKTKDSKVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1B9P3</accession>
    <name>A0A9Q1B9P3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_41501</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8019776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1B9P3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030417">
        <property type="entry name" value="MS4A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23320">
        <property type="entry name" value="MEMBRANE-SPANNING 4-DOMAINS SUBFAMILY A MS4A -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23320:SF130">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 212"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="87"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="116"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="256"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="260"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8019776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="284" mass="31454" checksum="46CC710ACF4F3AD8" modified="2023-09-13" version="1">MNTIQSSYKSTGSTIQLSLRNHVENNTKYTNKAIVSGQKWCAIAQLIIGIVSTVSGIITISTSRGNINKEYPAIFPQKCRAIDNSSYGVWSGMIYITTGVLGAYRRHNIKKIPCYIGFSVFSCVVSVSGIVIMMYYFAFCSGCMENDGLKAKVFFRFTSYAVLVLAHSNGMTFSIIGASLSCCGMECCNQSTTMEEFMSFLQDNTKADTLRMCPKPPSSPATTSAYNVTDITDVEQIYFEADNPINKAPLSYISTEEETEQKLRSKNTEKPSKRYHGVKLLPTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BD74</accession>
    <name>A0A9Q1BD74_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Sulfotransferase family cytosolic 1B member 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_35846</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BD74"/>
    <dbReference type="OrthoDB" id="205623at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sulfotransferase" evidence="1">
        <location>
            <begin position="34"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8023406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="102" mass="11684" checksum="8E802C93FE7A57F7" modified="2023-09-13" version="1">MRAAGPGLRRMKNKPSPRVLITHVPYALLPKGLYEKMCKNPLEAVKQIAAFLDKDLDNEALSRVVQHSSIEAMRKAYDQDHASSEKQEIEKNDFRFFIDRGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BE92</accession>
    <name>A0A9Q1BE92_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_35864</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BE92"/>
    <dbReference type="OrthoDB" id="10071849at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030417">
        <property type="entry name" value="MS4A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23320">
        <property type="entry name" value="MEMBRANE-SPANNING 4-DOMAINS SUBFAMILY A MS4A -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23320:SF130">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 212"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="138"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="198"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="233"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="262"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="303"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8023420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="358" mass="39434" checksum="BA5E0F4D83B3F737" modified="2023-09-13" version="1">MDTNQQGVAWVKDTERVITGDTSSDTHLRGSKARLISAIIHIVCGFVEFALGIAILLVPFTARFYPTKYVGWGIWNGAFACVTGIYGIYSRRNKCLITTYIVLAIIGAVFSCASLIWASLTATKIKIYASRRDYFEQYFMYITLCVAFGVQMVTSVIGVGFSCFDVCSANLNGSQNQEKIKINPDATSSKLPGASVRLICGIFQILFGVVQVALGITLLFLPLYYFDRADSKGWGIWNGVVAFATGLMGISSGRGKCRIKIYLVSAFVATLLSATCFISHWVILSFLTRPDESRHGHAYVRHVVIYLILCVSFGLQMLVSMIGFGHSCGAACSKDDGFDIKNAVYRYDRQDKSSQQLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BEE2</accession>
    <name>A0A9Q1BEE2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Complex I-SGDH</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">NADH-ubiquinone oxidoreductase SGDH subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HOLleu_38817</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Nanhai2018</strain>
            <tissue evidence="18">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Complex I is composed of 45 different subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complex I NDUFB5 subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BEE2"/>
    <dbReference type="OrthoDB" id="9995605at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019173">
        <property type="entry name" value="NADH_UbQ_OxRdtase_B5_su"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13178:SF0">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 BETA SUBCOMPLEX SUBUNIT 5, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13178">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE SGDH SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09781">
        <property type="entry name" value="NDUF_B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0249">Electron transport</keyword>
    <keyword evidence="14 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="8 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="71"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00032395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00032550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ8021572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="180" mass="20586" checksum="5C3825372E64F633" modified="2023-09-13" version="1">MAAHILRLLPQRGCIALRISSAGKTDVTTLLSKCNNLKAGAQLVRYAGGHGPQKMYIQPGTFQDWRAMRAFGFYLLLTGIPVAVIVTYINVFIGQATYAEIPEGYVPENYEYYRHPISRFIARHITESPQQSYEKMAHFIAIEGEKMKERKMEKEVRKAMRQRGDGPWFYTEIQGPPKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BEF1</accession>
    <name>A0A9Q1BEF1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">UPF0565 protein C2orf69-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_34739</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BEF1"/>
    <dbReference type="OrthoDB" id="419333at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018881">
        <property type="entry name" value="C2orf69_mit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31296:SF1">
        <property type="entry name" value="MITOCHONDRIAL PROTEIN C2ORF69"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31296">
        <property type="entry name" value="UPF0565 PROTEIN C2ORF69"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10561">
        <property type="entry name" value="C2orf69"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8024741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="344" mass="39461" checksum="DB7B97A7223C8B18" modified="2023-09-13" version="1">MYRATQREGSWLKSLLRISNGFLPHMAKLIRNTAPSANKECSIEMPNLIMLDKVEGHPGRRNTMLVTVPNVRITNGLHNIEQHVLFFPGDVQNMYEKMCDSFCGRDWKDWSYEAVSAILCKKFPLSIIWLVHPSRIHDQMFSCYDNFLECSIIGAPESHSRFEAVEHIDALLKQGCELLVMQHLDLLNEKDGNQEVSSKQPLKQALLKLPVSLIGFSKGCVVLNQLVYELEMWMTSSYTDKGTTQPNFLEKVKSFYWLDGGHTELTTKVWITEKKVLTPLANLGSEIQVHVTPWQIHDSSRPHIGEQERIFVDTLKALGANVTEHIHFPDEKKFLNHFKLLAEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BEF5</accession>
    <name>A0A9Q1BEF5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_38852</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BEF5"/>
    <dbReference type="OrthoDB" id="10677134at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040156227" description="Ig-like domain-containing protein" evidence="3">
        <location>
            <begin position="26"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="345"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="399"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="406"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8021602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="458" mass="50691" checksum="56CD62D4CE210FA7" modified="2023-09-13" version="1">MQSVLAVYVCMYATLCLLLGRVSYSEPFVSLTFYGPRILKINVKQYVLLGESDVTVGCSIEDLPATETNGTIHLYQNNQPVEDVVMSDGNLQLIWNGRPTFSFGSYSSFACEVYLTSDTFTSNIRGSEVVLQQYLEGSDTFMCLPTGAGFLSNEVITIVCLSERGASWSRETEDVLLGTDDNREIGNNFHVTTNNILLKTDFMNAGCRRDGPKGGMCSLELQPTVLNIDIIPDSFADNISFDRIVFTCLSNVPTDRISWRIDSANGNILDFDHVKGSRVNLSITHSFYASQLTVETQVENVISVSCKTSRSGHVAVATVNNNAIRTIIPSSTSVPTQNVSSTQTFHFIIIIVILVVALFFVILFLIFQKLRQSQEKVMGNQVVPRPTADTLPSDNPYYYATSYDAHNPETKNDANKDGNKLEKRSPRPQKRSETVDHLTTDVLGEGAVITLNEAYQIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BF30</accession>
    <name>A0A9Q1BF30_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_37879</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BF30"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8022864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="61" mass="6756" checksum="F2A84C3A1C01EB39" modified="2023-09-13" version="1">MESSREFNFNAAFLHRDHRLALSLFARGTSSYATAGLQVYYCTIVNVHALPALGPDQANIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BFC7</accession>
    <name>A0A9Q1BFC7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Retinol dehydrogenase 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_38230</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8023134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BFC7"/>
    <dbReference type="OrthoDB" id="5296at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008202">
        <property type="term" value="P:steroid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43313:SF50">
        <property type="entry name" value="GH26015P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43313">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE/REDUCTASE FAMILY 9C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="326"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8023134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="636" mass="71618" checksum="3ADADC6747E7595F" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BGQ8</accession>
    <name>A0A9Q1BGQ8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">YqaJ viral recombinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_36583</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BGQ8"/>
    <dbReference type="OrthoDB" id="6158042at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd22343">
        <property type="entry name" value="PDDEXK_lambda_exonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011604">
        <property type="entry name" value="PDDEXK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019080">
        <property type="entry name" value="YqaJ_viral_recombinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47526">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47526:SF3">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09588">
        <property type="entry name" value="YqaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YqaJ viral recombinase" evidence="1">
        <location>
            <begin position="9"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8023987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="192" mass="22409" checksum="0A532F1474643FA3" modified="2023-09-13" version="1">MGYYGSPNAPSLHWVRCMEDVARKHYLAHHRLNTKHRSVSWQPTGLWVSIQCPFIAASPDGIISCQQCGKGLLEIKNPYKYKNSTIHEFTSKVDTCLIHCNGVTHLDRKHDYYYQIQFQLWVTGFQWCDFVLRTNSQMDNIFVERLVQDNNFISNILPKLNYFFYYGIIPELKSRSVQGLVILNAVLKQCNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BGX5</accession>
    <name>A0A9Q1BGX5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Plexin domain-containing protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_34718</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BGX5"/>
    <dbReference type="OrthoDB" id="6285106at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031152">
        <property type="entry name" value="PLXDC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13055:SF12">
        <property type="entry name" value="LD40707P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13055">
        <property type="entry name" value="TUMOR ENDOTHELIAL MARKER 7 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="314"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="376"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="388"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8024724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="409" mass="45251" checksum="DD480124B0790731" modified="2023-09-13" version="1">MAESHCVQVDVCHDASHIQDDHNYYNSSYFPSGADYYIDLETEEPYFTTVTHGGLSSAHLKAAPVKLPFTFPFYGHNITTAYIATGGFLYVGSFVHEFLTVTQYIAPLMGNFDSSINDTHSIIRYANNGTAFTVEWKNNHINHMEDAGGFTFQTTLMSDGRIIFAYKNIPIPVDNIRGGNGHSVTVGLSDGYYIDEPLYEGTTLYKRTIYTYHVVSMDTRKVKDNAAFVFYPLTTCNQLLTCDTCTHDAATINFDCSWCSHIDRCSSGFDRHREEWIENECDLIAVYSDCPQSSSQADPIQVVSIAPRKESGDLLGIIIGLVVITLVIVLLGSVTCWVAYACRNPTSPSGLFIIEAQSFAKGKFSKDTSNEDRYIVSNGNEESETKTDVISANGHSTPVVNTTQSTSEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BH05</accession>
    <name>A0A9Q1BH05_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Muscarinic acetylcholine receptor gar-3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_33973</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8026195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BH05"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016907">
        <property type="term" value="F:G protein-coupled acetylcholine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004993">
        <property type="term" value="F:G protein-coupled serotonin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007197">
        <property type="term" value="P:adenylate cyclase-inhibiting G protein-coupled acetylcholine receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007187">
        <property type="term" value="P:G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00637">
        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24247">
        <property type="entry name" value="5-HYDROXYTRYPTAMINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24247:SF265">
        <property type="entry name" value="MUSCARINIC ACETYLCHOLINE RECEPTOR DM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="22"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="63"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="357"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="389"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="10">
        <location>
            <begin position="1"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8026195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="438" mass="49714" checksum="6F7C25E2A8CE173F" modified="2023-09-13" version="1">MMAELLEYAKPSWRGSFELCHLTFWSSYILLYSSVYMVVFISRDRYLLVKDVIRHRLTQTPKTALKLIAGSYVIIVGFTTSIFVWLVLSDNLKQFCASQYLKHRKFQTFMVFVDIVIPVTLLLGYNVLMLKGLKGRRSELRSMMVTPCNHKQSSSNHGHSKSKMKLSITPSIPESINYATESLATLEGSSQRTHDKELVDATGHVRHNIDVSFLSAENNWIQLPILNLLIVNIPLVPASAKIRANRRNINPCFKEGKFVQFRENESMDNSNKFSSLGAQSSHPVSDIIISESDGCAHDEWKNIAHSFEEIKADVISETVSISAEKTKAAKGRAENFTCAECKAMQRKQRELDGCKKAVKTVTTLVCVFLICWLPYYIAAFLKVYDVVNVSYTVSTLMFVILCSNSAINPCLYVVTHNGFRHVILQLFPLTVLRRCRRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BH17</accession>
    <name>A0A9Q1BH17_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Aryl hydrocarbon receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_33097</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BH17"/>
    <dbReference type="OrthoDB" id="6099906at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071456">
        <property type="term" value="P:cellular response to hypoxia"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23043">
        <property type="entry name" value="HYPOXIA-INDUCIBLE FACTOR 1 ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23043:SF17">
        <property type="entry name" value="PROTEIN SIMILAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14598">
        <property type="entry name" value="PAS_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="PAS" evidence="7">
        <location>
            <begin position="38"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="7">
        <location>
            <begin position="199"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="314"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="367"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="314"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="368"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8025518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="694" mass="78989" checksum="405E6D89226383D5" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BH18</accession>
    <name>A0A9Q1BH18_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_34033</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8026240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BH18"/>
    <dbReference type="OrthoDB" id="418245at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8026240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="91" mass="9973" checksum="BFA8602DEE82613E" modified="2023-09-13" version="1">MVALERGGVLPYMPFTCYTTLSPARDGATMCRKDWTQYGNECFYYNTNSLSVSDAQAYCETQGGNLASIISSGARDTIRNMISTTAHIGKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BJE9</accession>
    <name>A0A9Q1BJE9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_32799</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BJE9"/>
    <dbReference type="OrthoDB" id="6155329at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005312">
        <property type="entry name" value="DUF1759"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22954:SF3">
        <property type="entry name" value="PROTEIN CBG08539"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22954">
        <property type="entry name" value="RETROVIRAL PROTEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03564">
        <property type="entry name" value="DUF1759"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="11"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8027605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="172" mass="19440" checksum="AF21941D97D29592" modified="2023-09-13" version="1">MDIVKVEGVTVEEINLRVEEFDKRLKALDEIQEQIQELITEEDDLLKEIEQASTVRNKAFFCKSEAVRVIGQIKASSTGPSVSTGKSLSSTRLPKLELPKFSGDITTWQTFWDKFVAIVDISDIPVINKFTYLQSLLEGDALLSIQGLSLTDSHYRLACYILKESNGRKERI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BKE4</accession>
    <name>A0A9Q1BKE4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Transient-receptor-potential-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_30343</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BKE4"/>
    <dbReference type="OrthoDB" id="2373987at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034703">
        <property type="term" value="C:cation channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070679">
        <property type="term" value="F:inositol 1,4,5 trisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015279">
        <property type="term" value="F:store-operated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051480">
        <property type="term" value="P:regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007338">
        <property type="term" value="P:single fertilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013555">
        <property type="entry name" value="TRP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002153">
        <property type="entry name" value="TRPC_channel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00870">
        <property type="entry name" value="trp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10117">
        <property type="entry name" value="TRANSIENT RECEPTOR POTENTIAL CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10117:SF80">
        <property type="entry name" value="TRANSIENT-RECEPTOR-POTENTIAL-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TRP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01097">
        <property type="entry name" value="TRNSRECEPTRP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01420">
        <property type="entry name" value="TRP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 10" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="9" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="371"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="410"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="439"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="477"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="521"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="555"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="617"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="647"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="10">
        <location>
            <begin position="170"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="Transient receptor ion channel" evidence="13">
        <location>
            <begin position="205"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="848"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="986"/>
            <end position="1007"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1024"/>
            <end position="1053"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="991"/>
            <end position="1000"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1038"/>
            <end position="1053"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ8028176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1053" mass="119892" checksum="CFD7867C2EBD3397" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BNL5</accession>
    <name>A0A9Q1BNL5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PWWP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_29385</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PWWP_dom"/>
    </dbReference>
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        <property type="entry name" value="MUM1 LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="332"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="domain" description="PWWP" evidence="3">
        <location>
            <begin position="509"/>
            <end position="631"/>
        </location>
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        <location>
            <begin position="26"/>
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        </location>
    </feature>
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        <location>
            <begin position="187"/>
            <end position="250"/>
        </location>
    </feature>
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        <location>
            <begin position="265"/>
            <end position="310"/>
        </location>
    </feature>
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        <location>
            <begin position="443"/>
            <end position="482"/>
        </location>
    </feature>
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        <location>
            <begin position="38"/>
            <end position="51"/>
        </location>
    </feature>
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        <location>
            <begin position="58"/>
            <end position="68"/>
        </location>
    </feature>
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        <location>
            <begin position="69"/>
            <end position="82"/>
        </location>
    </feature>
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        <location>
            <begin position="187"/>
            <end position="202"/>
        </location>
    </feature>
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        <location>
            <begin position="218"/>
            <end position="233"/>
        </location>
    </feature>
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        <location>
            <begin position="234"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="265"/>
            <end position="283"/>
        </location>
    </feature>
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        <location>
            <begin position="299"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="453"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF20886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8029873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="644" mass="73314" checksum="A9F832A3095D9EA3" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BPM2</accession>
    <name>A0A9Q1BPM2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_26934</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BPM2"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8030491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="331" mass="37683" checksum="4FA9A2CA8F3EFCE0" modified="2023-09-13" version="1">MSLPAMVLRQVQTVSNKQLSISAMSSRIIAQSILWFFLLFYYDAYGTITENVVESIFWDGIGAYIPVDRQIIQKKLDTWYDSDEKICKVAPKLYLPETRVFPNDLNESQHVVFIAPAYSKLNDFQEMHDPCLSLDGIELTVTIPLLTDDPKGHPKYALALGQFYKQDRGDLAKPNLVYPYIPVEEITMGNNTFIMKNGSDVLRLTFQKFTDPCEPPSPVVLHEFDEFAKQEFHFGRPEPHFSICDGLTINNNYCSTAERIRCLVHRSSPNICQTSWNVLDRKVCKSSLDVTNITSGFRAFMYLPDEPLVTIASQTVRAHFSIGLKFPCIDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BPV0</accession>
    <name>A0A9Q1BPV0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Protein FORGETTER 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_27375</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SBNO family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="421838at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000342">
        <property type="entry name" value="Protein strawberry notch homolog 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057332">
        <property type="entry name" value="SBNO_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026937">
        <property type="entry name" value="SBNO_Helicase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026741">
        <property type="entry name" value="SNO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039187">
        <property type="entry name" value="SNO_AAA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12706:SF33">
        <property type="entry name" value="PROTEIN WITH HELICASE_C DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12706">
        <property type="entry name" value="STRAWBERRY NOTCH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13872">
        <property type="entry name" value="AAA_34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13871">
        <property type="entry name" value="Helicase_C_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25373">
        <property type="entry name" value="SBNO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Strawberry notch AAA" evidence="6">
        <location>
            <begin position="139"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="domain" description="Strawberry notch helicase C" evidence="5">
        <location>
            <begin position="593"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="domain" description="SBNO alpha/beta" evidence="7">
        <location>
            <begin position="914"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="11"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="617"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1159"/>
            <end position="1277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="11"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="624"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1177"/>
            <end position="1190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="1191"/>
            <end position="1201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1205"/>
            <end position="1223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1224"/>
            <end position="1238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1247"/>
            <end position="1257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF25373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8030843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1339" mass="148974" checksum="14B095C1CE652D31" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BRB5</accession>
    <name>A0A9Q1BRB5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_24547</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BRB5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8031376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="117" mass="13301" checksum="772D607FA0CE61A0" modified="2023-09-13" version="1">MLASCSSKSHSIIDFSTEKAINFTDIILLEKSQKAVKTWIFLEQLTFLQLVTHSSCSIYQKAAVLCKFVQRCFFGINPQTGSKSVKEKGGKKNHIQPGVLRLVNKIKDFEFKTEWDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BRF9</accession>
    <name>A0A9Q1BRF9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_27903</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8031236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BRF9"/>
    <dbReference type="OrthoDB" id="8962223at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000032">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 297-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon gypsy-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF13">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="2">
        <location>
            <begin position="857"/>
            <end position="1015"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="325"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="591"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="690"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="325"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="340"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="364"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="591"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="634"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="659"/>
            <end position="673"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8031236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1129" mass="128693" checksum="DB921D282E912FB8" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BRQ3</accession>
    <name>A0A9Q1BRQ3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Aggrecan core protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_24616</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BRQ3"/>
    <dbReference type="OrthoDB" id="418245at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050111">
        <property type="entry name" value="C-type_lectin/snaclec_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22803">
        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040305947" evidence="1">
        <location>
            <begin position="23"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="2">
        <location>
            <begin position="34"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8031430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="177" mass="20363" checksum="7981066755269D88" modified="2023-09-13" version="1">MKIQLLALATTVALFSTQFANGYQVVCSEGWTQWKGGCYRFYNQPRSSWEEAQAFCNNQQLSCNICPEETAHLVTIESEEENDFLFNWWKSLRDPLPASTPQSFWIDGNDKQSEGTFVLTNGKTLDYFNWIIEQPNNWNNQDCLAMAKAPNLNSDVGKWNDAGCSVDYPFMCEFRCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BRU3</accession>
    <name>A0A9Q1BRU3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_24696</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BRU3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040376976" description="Ig-like domain-containing protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="38"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8031490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="257" mass="28472" checksum="950646332D77021C" modified="2023-09-13" version="1">MDNATLWTTYTIIIISCFVQGHSTACSDYSSEVTGVLGENVSVSCTFQRYCEHLFWRNESSQLYIAHIGSTQTTAVKSYVDVRGDTSVLYIRQSTFDDAGFYKCFCFVSGIPTGDDICYDLQLICQIKVNVNGELMIFKGSLPSRFSLHVVNVTENDKVIIDCPDGTHLQRKCTDGTDQQSFQFTVTSSYHNCQLSCITPTSSRELASRCHDISMKLQVSSNGMSTAHPGFLLTSGESKRITVLAMSFLVLTCTILV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BRX7</accession>
    <name>A0A9Q1BRX7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_24957</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BRX7"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8031687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="62" mass="6992" checksum="8ADC86E0B7E06773" modified="2023-09-13" version="1">MESSREFNFNAAFLELSRTLSLFARGTSSYATARCRSTTVPSSTCMHYQPLDQTRQILIKGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BUB7</accession>
    <name>A0A9Q1BUB7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Kremen protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_23008</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8032917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BUB7"/>
    <dbReference type="OrthoDB" id="5985073at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051836">
        <property type="entry name" value="Kremen_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002889">
        <property type="entry name" value="WSC_carb-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24269">
        <property type="entry name" value="KREMEN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24269:SF16">
        <property type="entry name" value="PROTEIN SLG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01822">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00321">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51212">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="306"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="WSC" evidence="11">
        <location>
            <begin position="45"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="10">
        <location>
            <begin position="141"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8032917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="423" mass="47062" checksum="2267CD6610F71D48" modified="2023-09-13" version="1">MQVRGDILADSITLTANCRESDSTKECVFVTTSKCASQIHISCQFPGFLGCFNHQNSSSALFKVLSSFTEFTIQKCIEECREEGYDFAGVTQGATCLCGDTIASRQLDNYLCQAFPCRDDTYQSCGGGSEISIYGTSFGKCGGHIGYPSNRGNTLQYLVSPNYPESSEEVDCRWTILFQKRTAEVQVIFIDLLLRQASFLKFSYLLSNGYVGEQYLMNTDDRPVEFVQNFEEVPIVSMTVQLSSPSNESLACFAMYYKANLLATIKMPSASSEQLNTSSYITSSITSVKVSATEEMPPTKLNLNFVYGWLILGLFVITTWASISLKIPCKTHKREDGESSHSVRNHARRSKQVSLSSIIDDDVNSVHLNRIVSDDTSVSHGNIVELAPEQQKGHYYIENESGKKIICKSQEPTEVTTDDKRED</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BWI0</accession>
    <name>A0A9Q1BWI0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_24194</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BWI0"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8033831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="102" mass="11249" checksum="FC7E28B686CF6151" modified="2023-09-13" version="1">MASGNRVTVNTTTSGCVTNEELNIHIQYGFYDRHYLESVDSSDADKLPDLEEALETKTNDVQVYFSTGLCNESVETAARNDATTVPVKMKMILGSLMGYLSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BWU4</accession>
    <name>A0A9Q1BWU4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_24456</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8034039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BWU4"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="163"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="194"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8034039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="225" mass="24762" checksum="E3DEB89775C74E4C" modified="2023-09-13" version="1">MALTTHSSLGHNQQPSMSFKVGCYHCFPFFVLDVTKETENEEVPLIEEKEDLKLRVHAEARDEPDGAPVETKVVEEVKEEKPEEPSPEETAPPAETAPPEERAPPEETVPLEEALPSEEIAPAVLESAPPKEPETTPEEQTGEAEDTSEAVKDDQVGEVAENTEEKPEETVGEGDGHKEEETTENTDDMKEEKSEEENPQTEEPEVAKPEDEQENVPTEAAPEGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BX24</accession>
    <name>A0A9Q1BX24_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_20963</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BX24"/>
    <dbReference type="OrthoDB" id="5803930at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="129"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="144"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="165"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8034220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="247" mass="28165" checksum="984B6C32F79FA859" modified="2023-09-13" version="1">MDYLSSEEEELMMAALDRVERLERAKKQQAAFREKWAARQRPERSEEGGMAGEPSQSNQFSPTSEKLEEAKLRDEGVARKRRNPENEFDVSCEDEEEQNAARESHPNPGKGSNLKHHMRPGGSCAAKRQRLEEVPDRSGEPASGEQSGSSTVTTGPPYESSTESENSRIESVEANRSVVEDRGLEIGGGEDNRENDVLKEDPIAITEEVRREMRDMMCDMYEKNWGAIRTHHRRHNRVQDVYNFRLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BY57</accession>
    <name>A0A9Q1BY57_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Craniofacial development protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_21984</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BY57"/>
    <dbReference type="OrthoDB" id="410381at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027124">
        <property type="entry name" value="Swc5/CFDP1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23227">
        <property type="entry name" value="BUCENTAUR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23227:SF84">
        <property type="entry name" value="ENDONUCLEASE_EXONUCLEASE_PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8034945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="376" mass="42748" checksum="F6A1B67B53986FFA" modified="2023-09-13" version="1">MVVVILFFWSGRDSSERKETGVGFAVRNNLVRKLLMLPEGLNDRLLSSKKNTTVISVYAPTMTNPMDVKGRFYEVLDLLIASVPQTDKLVLFGDFNARVGSYYQTWSGVIGMHGVGKCNSNGLLLLKTCATHNLSITNTMFRLPTRYTNSWMHPRSRNWHLIDYVVIRKDDRNDVRVTKAMCGGDCWTDHLLIIFKLNLHIKPRRRPQGQRTPRRLDISKEKVEEVAKNLSSALEQRIWNQQMSTTTSKNNGQWSEISLTQQLLKILDQINKALNTVYGPKSSESSPVLNADGTKLLTDRKQVLERWAEHFNSVLNRPSSISNDAIDRFPQVKTDYTPDDLPTEHEVEKAIHQLSCGKAPGSDSIPAEVFKVFKVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BZQ9</accession>
    <name>A0A9Q1BZQ9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Sulfotransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_19546</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WSCD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8035770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BZQ9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051589">
        <property type="entry name" value="Sialate-O-sulfotransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45964">
        <property type="entry name" value="WSCD FAMILY MEMBER CG9164"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45964:SF5">
        <property type="entry name" value="WSCD FAMILY MEMBER CG9164"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8035770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="280" mass="32354" checksum="BFBB0BF68E68664E" modified="2023-09-13" version="1">MGHIFKLIHVAFACVYCFLLLEIVRTLYLSFETRAGYNKRSYPRIPLEDYARKHISRETNFTVEFSGIINLSENRKVNVEDGAKNLTVSNSNEANVHAISARDKCADSNLETISPVPEPPPFTALASYPRSGNAYTGLLIQISTQFHTSSVYWRNERKKPIAQKAWVEKSVQIVEGWKRFNMDWLLKGKRVLVVSFERLCDNTLEELEKIVKFLNQPVRLHQIQCAVTLQPCKCRDRVDFEPYYVDTVREYIAELNETLIRDYSTSLPTYGTKCPFDKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C0B9</accession>
    <name>A0A9Q1C0B9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">exodeoxyribonuclease III</fullName>
            <ecNumber evidence="3">3.1.11.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_19619</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.</text>
            <dbReference type="EC" id="3.1.11.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="7">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="7">Probably binds two magnesium or manganese ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA repair enzymes AP/ExoA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.11.2" evidence="3"/>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8035824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C0B9"/>
    <dbReference type="OrthoDB" id="8961218at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003906">
        <property type="term" value="F:DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008311">
        <property type="term" value="F:double-stranded DNA 3'-5' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09076">
        <property type="entry name" value="L1-EN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004808">
        <property type="entry name" value="AP_endonuc_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748">
        <property type="entry name" value="AP ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748:SF26">
        <property type="entry name" value="ENDONUCLEASE_EXONUCLEASE_PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0464">Manganese</keyword>
    <keyword evidence="4 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="8">
        <location>
            <begin position="6"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR604808-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8035824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="173" mass="19579" checksum="4FA96187D184A675" modified="2023-09-13" version="1">MAIKIGSYNARGLRQPSKRRQQFAYLHRHHFDIVFIQETHSTSSDDRFWCNEWGGNILFSHGTSSSRGVAIFFRPGTSFDILKIHCDNSGRFVVADVGYGGSIFTLLSVYGPNLDSPNFYSNVSTCLSNFQCDNMICGGDFNFVFNLDLDKEGGARRTNFNARKDCFTLMEKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C0P9</accession>
    <name>A0A9Q1C0P9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Fibrillin-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_20442</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="4405280at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
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        <property type="match status" value="2"/>
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        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="10"/>
    </dbReference>
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        <property type="match status" value="22"/>
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    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
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    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040406155" evidence="11">
        <location>
            <begin position="21"/>
            <end position="1659"/>
        </location>
    </feature>
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        <location>
            <begin position="1522"/>
            <end position="1549"/>
        </location>
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        <location>
            <begin position="31"/>
            <end position="151"/>
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            <begin position="154"/>
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    <feature type="domain" description="EGF-like" evidence="12">
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            <begin position="195"/>
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            <begin position="275"/>
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        <location>
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        <location>
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        <location>
            <begin position="600"/>
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            <begin position="1630"/>
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            <dbReference type="ARBA" id="ARBA00023180"/>
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            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
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        </source>
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        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
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        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAJ8036461.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1659" mass="178818" checksum="39E226CF0D67FA9E" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C0T6</accession>
    <name>A0A9Q1C0T6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_19952</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C0T6"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8036084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="63" mass="7494" checksum="528DC98405F27F89" modified="2023-09-13" version="1">MESQEEETKLTENQIDEIRKFLEKLFEEKQKGLRNKSGLNQYQFRTSTYGGLHSQQHHLHSTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C0V9</accession>
    <name>A0A9Q1C0V9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Ras-related protein Rab-8A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_20242</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endomembrane system</location>
            <topology evidence="7">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01867">
        <property type="entry name" value="Rab8_Rab10_Rab13_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ras-related protein Rab-8A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050305">
        <property type="entry name" value="Small_GTPase_Rab"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47980">
        <property type="entry name" value="LD44762P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0636">Prenylation</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8036307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="208" mass="23636" checksum="9660179E7102FCA4" modified="2023-09-13" version="1">MMAKTYDFLFKLLLIGDSGVGKTCVLFRFSEDAFNATFISTIGIDFKIRTIELDGKKIKLQIWDTAGQERFRTITTAYYRGAMGIMLVYDITNLKSFDNIRNWIRNIEEHASADVEKMILGNKCDMDDKRQVSKEKGEKLAIEYGIKFMETSAKASINVEEAFVTLARDIKAKMDRKLIASGDQNSGQVKVNQGNAQKRSVWKMCSIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C297</accession>
    <name>A0A9Q1C297_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">U7 snRNA-associated Sm-like protein LSm11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_17780</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C297"/>
    <dbReference type="OrthoDB" id="10002367at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005683">
        <property type="term" value="C:U7 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071209">
        <property type="term" value="F:U7 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006398">
        <property type="term" value="P:mRNA 3'-end processing by stem-loop binding and cleavage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039267">
        <property type="entry name" value="Lsm11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21415">
        <property type="entry name" value="U7 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21415:SF1">
        <property type="entry name" value="U7 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8037057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="367" mass="41564" checksum="459444F8DB284F7D" modified="2023-09-13" version="1">MAAPWKSGAPSAESDYCEETDVFSANFDPLRAIYDENIRLPFPEAEPFTNISKFVDHLNRQTDTGQCSRLPVDEPKRSDLTAARGRDKNDSVQVQISTEEKRQTRRVKTVLNVMEDADGPLSLLAWSKENSKKVKVCTRTFKGLRGICIGYIEAFDKFLNLALVDVDEIYRKPPLGQTIYHQEILTFSKIMEHFETRKKLGVTKKPEKVEKKKRNRKERKLRLEEKLKAEGVFIENYHPPPVSGDPVTDVDREGNAVTEASSGKVLTNDAERIARLQRLTESMLSSSDETMMESTSDKTAIQMESCVIPGESLVAPEESGVMKGSTDQSSKKKGKDVIKHEDFLWRHVRQLFVRGDSIVLIAVDKNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C3L2</accession>
    <name>A0A9Q1C3L2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_18156</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C3L2"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8037356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="160" mass="18042" checksum="410B2406750D770A" modified="2023-09-13" version="1">MAVSMATGGYPTSNSLDHSVFDVFFTNEDCKGLRRNLINSTDDEINKFVTPLITRVRALYLQPLVALVGILGNLAFMFIVWKLIVVCNCGATTRNENDSETMLPPLTTDCRHCTLATLRNHWYDCRLLLIPIPHCEVTLKPKFSWVVVSTQFSLMWDEGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C525</accession>
    <name>A0A9Q1C525_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_15702</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C525"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8038315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="87" mass="9644" checksum="A4C4F71D63CE464F" modified="2023-09-13" version="1">MTWKTARASPDRGNHPLVANDTNEDTTNEKDACAGCDELKDEVQTVTPLSTGICSKWQIYRYSTNDDITVENNESVTTEELSGESNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C6I6</accession>
    <name>A0A9Q1C6I6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phospholipase A2</fullName>
            <ecNumber evidence="8">3.1.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_16644</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1-acyl-sn-glycero-3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15801"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="5">Binds 1 Ca(2+) ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the phospholipase A2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.4" evidence="8"/>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C6I6"/>
    <dbReference type="OrthoDB" id="5841574at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047498">
        <property type="term" value="F:calcium-dependent phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050482">
        <property type="term" value="P:arachidonate secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00125">
        <property type="entry name" value="PLA2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.90.10:FF:000015">
        <property type="entry name" value="Phospholipase A(2)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.90.10">
        <property type="entry name" value="Phospholipase A2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001211">
        <property type="entry name" value="PLA2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016090">
        <property type="entry name" value="PLA2-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033112">
        <property type="entry name" value="PLA2_Asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033113">
        <property type="entry name" value="PLA2_histidine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036444">
        <property type="entry name" value="PLipase_A2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11716:SF51">
        <property type="entry name" value="PHOSPHOLIPASE A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11716">
        <property type="entry name" value="PHOSPHOLIPASE A2 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00068">
        <property type="entry name" value="Phospholip_A2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00389">
        <property type="entry name" value="PHPHLIPASEA2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00085">
        <property type="entry name" value="PA2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48619">
        <property type="entry name" value="Phospholipase A2, PLA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00119">
        <property type="entry name" value="PA2_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00118">
        <property type="entry name" value="PA2_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0106">Calcium</keyword>
    <keyword evidence="3 6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040529319" description="Phospholipase A2" evidence="8">
        <location>
            <begin position="17"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipase A2-like central" evidence="9">
        <location>
            <begin position="24"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="70"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="123"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="51"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="66"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="73"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="83"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="109"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR601211-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR601211-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR601211-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8039054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="147" mass="16692" checksum="CECA204F3ADB3A97" modified="2023-09-13" version="1">MRSALVFSLLFSLAFASSLRAKRSTLQFREMIHCLTDRRAYLDYDSYGCWCGLRGKGNPVDATDRCCKTHDGCYDDLKKSGVCRGWDFFLKGYSYSKRNCDTSTASVTCGGGNDCEVGLCRCDRAAAICFRRSAFNPMYRKWNLRKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C6K3</accession>
    <name>A0A9Q1C6K3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_17354</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C6K3"/>
    <dbReference type="OrthoDB" id="10070632at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="64"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8039587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="122" mass="13562" checksum="30FA455E3C258451" modified="2023-09-13" version="1">MSHKKDVMQKLVGRQSVHGSTEERRKNKQRQALYQIRQDISEARFETNEAITQIHKGVNKLEQSSSNTGSFDSSGEKEYGSSETLRAMQDLLQKKQEPSSSTKGETSGATSQVENHTSSAST</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C6Y2</accession>
    <name>A0A9Q1C6Y2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HOLleu_14752</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Nanhai2018</strain>
            <tissue evidence="15">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 17">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6058203at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00055">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00047">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000102">
        <property type="entry name" value="Receptor-type tyrosine-protein phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.300.10:FF:000003">
        <property type="entry name" value="tyrosine-protein kinase receptor Tie-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.300.10">
        <property type="entry name" value="Tie2 ligand-binding domain superfamily"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002049">
        <property type="entry name" value="LE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050348">
        <property type="entry name" value="Protein-Tyr_Phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134:SF560">
        <property type="entry name" value="PROTEIN-TYROSINE-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134">
        <property type="entry name" value="RECEPTOR-TYPE TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="15" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040146380" description="protein-tyrosine-phosphatase" evidence="10">
        <location>
            <begin position="29"/>
            <end position="1341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="676"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="11">
        <location>
            <begin position="261"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="14">
        <location>
            <begin position="546"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="12">
        <location>
            <begin position="791"/>
            <end position="1046"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="13">
        <location>
            <begin position="963"/>
            <end position="1037"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="12">
        <location>
            <begin position="1071"/>
            <end position="1330"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="13">
        <location>
            <begin position="1248"/>
            <end position="1321"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="287"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ8040458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1341" mass="152161" checksum="73A28B7F63928B8D" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C8Z8</accession>
    <name>A0A9Q1C8Z8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Bile salt sulfotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_14485</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sulfotransferase 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C8Z8"/>
    <dbReference type="OrthoDB" id="205623at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11783">
        <property type="entry name" value="SULFOTRANSFERASE SULT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sulfotransferase" evidence="3">
        <location>
            <begin position="16"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8040249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="136" mass="16134" checksum="A37116D130335A8B" modified="2023-09-13" version="1">MVTVLEFTWAPTPEKIKQTTPGYKIIEKLPSPRVIITHIPEPLCPPQWFTKRAKIIYFARNPKDVMVSSYNFMKPILNPKLRSWEAFFKYFCGDYGKEILTLNIWILNLETYVHYNEKLLLIFLFISNTFVICNVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CAB0</accession>
    <name>A0A9Q1CAB0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_15331</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CAB0"/>
    <dbReference type="OrthoDB" id="425681at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="121"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8040894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="139" mass="15751" checksum="9484EE7F4AE326E6" modified="2023-09-13" version="1">MGKKVHQQRGPHQGEAIHHLCYAHSTPPTLAWSCPSNGEGLYIQRTFCMDSRNVVLVPEERYRDSCKRNLQFAYININSWEDIASQRSIWLLAVKPGVNRAEKDRSENGPPNNRRGRHQSYHPTIFSSVTPAQKAATPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CCW0</accession>
    <name>A0A9Q1CCW0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Coiled-coil domain-containing protein 157</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_09398</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8042606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CCW0"/>
    <dbReference type="OrthoDB" id="10051906at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029681">
        <property type="entry name" value="CCDC157"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43696">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 157"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43696:SF9">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 157"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="640"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="675"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="747"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="145"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="389"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="584"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="645"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="675"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="691"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="709"/>
            <end position="720"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8042606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="818" mass="92527" checksum="4D321F84177B0EBB" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CEF9</accession>
    <name>A0A9Q1CEF9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase A2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_11069</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CEF9"/>
    <dbReference type="OrthoDB" id="10068942at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001995">
        <property type="entry name" value="Peptidase_A2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33198">
        <property type="entry name" value="ANK_REP_REGION DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33198:SF20">
        <property type="entry name" value="RETROTRANSPOSON GAG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50175">
        <property type="entry name" value="ASP_PROT_RETROV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase A2" evidence="2">
        <location>
            <begin position="306"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8043802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="388" mass="43149" checksum="ED48D3B1106E4D2C" modified="2023-09-13" version="1">MGYLSQPPPFLQSAGVPTVPWKQWISVFETYLLASGGEEYSAERKRAMLLHCIGVEGQRIFQTLPKLPDSVKLDTYQGAVKVLEDYFIPSVNVVAERYRFRQRAQKTGETIDCYVASLRELSKTCDFGPMTDEMIRDQIIEKTATPRVRERLLMEKDLKLDGAIETAKCIENAMKEAKAIGNSLTKETLEQDVYRLTSRGNKPNVTKTGTKTKRVCYRCGSTQHIASDPNCKARDKKCKNCKKIGHFAVMCRSKPIAQTKNGPPAYHIPSNPGDGNYEVLANDSNGRKGSKSKRIRCVVMINGVPIQLDVDTGSGVSILSEEVFSKSFLSEALVKVPEGIKLSSYTKHKIEVIGCFEATVSYLDRVANTIFFVVKSGSNLLGRDLIQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CEV6</accession>
    <name>A0A9Q1CEV6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Copia protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_11403</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8044051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CEV6"/>
    <dbReference type="OrthoDB" id="439192at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="CDD" id="cd09272">
        <property type="entry name" value="RNase_HI_RT_Ty1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11439:SF515">
        <property type="entry name" value="GAG-POL POLYPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11439">
        <property type="entry name" value="GAG-POL-RELATED RETROTRANSPOSON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8044051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="182" mass="20425" checksum="CB4B09D9F5D26601" modified="2023-09-13" version="1">MAKHVLRYVKGTLDQSLIFKRSDNPLQLISFCDGDWGNSADRKSVTGYIFQLASDGPVISWKSRKQQTVALSTCKAEYMSMAAARSQICMVNDILFDQNDKLFDQVTLFCNNKGAIALAKNPVLHQRSKHIDIKYHFIGSEVQKGVVKNVYVPSEQNVADILNKPLAKSKLQKFVSHLMGCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CFC4</accession>
    <name>A0A9Q1CFC4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF4430 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_10892</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CFC4"/>
    <dbReference type="OrthoDB" id="6343110at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.130.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051588">
        <property type="entry name" value="Cobalamin_Transport"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10559:SF18">
        <property type="entry name" value="TRANSCOBALAMIN II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10559">
        <property type="entry name" value="TRANSCOBALAMIN-1/GASTRIC INTRINSIC FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040148424" description="DUF4430 domain-containing protein" evidence="1">
        <location>
            <begin position="24"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8043688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="154" mass="17657" checksum="F4E1AF829D3BCA72" modified="2023-09-13" version="1">MDVAKIFLTTLMSYVYFFRSANGAFCGDPVAEYPEITPETELITCHLSVTSLADGPFPGVDHMSVKAPVNASVTQIMLLAEKHSGGDFWFESDYHENFGTYVHTINGLSNDDPYYWFLYDVMDDGQTNCGTDFTRARDNDHFHWLYEEYTYGYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CFJ7</accession>
    <name>A0A9Q1CFJ7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Golgi SNAP receptor complex member 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_11035</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GOSR1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CFJ7"/>
    <dbReference type="OrthoDB" id="422156at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005801">
        <property type="term" value="C:cis-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005797">
        <property type="term" value="C:Golgi medial cisterna"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048219">
        <property type="term" value="P:inter-Golgi cisterna vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15864">
        <property type="entry name" value="SNARE_GS28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023601">
        <property type="entry name" value="Golgi_SNAP_su1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21094:SF2">
        <property type="entry name" value="GOLGI SNAP RECEPTOR COMPLEX MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21094">
        <property type="entry name" value="GOS-28 SNARE- RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12352">
        <property type="entry name" value="V-SNARE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF027109">
        <property type="entry name" value="Golgi_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="165"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="9"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8043778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="208" mass="24099" checksum="E33C713F1AC85A9F" modified="2023-09-13" version="1">MFDTMAMEIEQLLGKLTEINDKMSEYTNTLSMTSPSSALLHTLQRHRDILQDYNQEFQKTKANITACREREELLGSVRRDIDAYKSSSGLNRRSEMYLKENEHIRNADRIADETIDVALATKENLREQRNTMKKISTGMNNLANRFPVLNSLIQRINIRKRRDTLIIGGVIAVCIILLLLYAFRKSRQRIQASRTFKQLQGTLKDFGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CHU1</accession>
    <name>A0A9Q1CHU1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">protein kinase C</fullName>
            <ecNumber evidence="5">2.7.11.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">HOLleu_07608</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="25">Nanhai2018</strain>
            <tissue evidence="25">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="25">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.13" evidence="5"/>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CHU1"/>
    <dbReference type="OrthoDB" id="10252171at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004697">
        <property type="term" value="F:diacylglycerol-dependent serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20795">
        <property type="entry name" value="C1_PKD_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01239">
        <property type="entry name" value="PH_PKD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14082">
        <property type="entry name" value="STKc_PKD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000151">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000021">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020454">
        <property type="entry name" value="DAG/PE-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057764">
        <property type="entry name" value="Ubiquitin_PRKD1-3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22968">
        <property type="entry name" value="PROTEIN KINASE C, MU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22968:SF24">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25525">
        <property type="entry name" value="Ubiquitin_PRKD1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00008">
        <property type="entry name" value="DAGPEDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14 25" id="KW-0418">Kinase</keyword>
    <keyword evidence="17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="18" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="15" id="KW-0862">Zinc</keyword>
    <keyword evidence="13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="24">
        <location>
            <begin position="130"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="24">
        <location>
            <begin position="246"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="22">
        <location>
            <begin position="369"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="535"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="302"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="489"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="824"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="21">
        <location>
            <begin position="314"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="490"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="512"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="838"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="564"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="KAJ8044764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="853" mass="96258" checksum="1562B8CC2A176703" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CHY9</accession>
    <name>A0A9Q1CHY9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_07854</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CHY9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8044949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="88" mass="9561" checksum="84609DF738C71D8D" modified="2023-09-13" version="1">MGVYLVATGDYFVVTHLPTGDLLPTGDVPGWFSCCNLFSAPFQLPSKSLSNIGCCPQYTYLVQTLCKTLHLNLPVVCQGSNRDVPSYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CKH4</accession>
    <name>A0A9Q1CKH4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">thioredoxin-disulfide reductase (NADPH)</fullName>
            <ecNumber evidence="4">1.8.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HOLleu_05020</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Nanhai2018</strain>
            <tissue evidence="18">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Has a glutathione-disulfide oxidoreductase activity in the presence of NADPH and glutathione reductase. Reduces low molecular weight disulfides and proteins.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.9" evidence="4"/>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CKH4"/>
    <dbReference type="OrthoDB" id="5956163at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004362">
        <property type="term" value="F:glutathione-disulfide reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004791">
        <property type="term" value="F:thioredoxin-disulfide reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03419">
        <property type="entry name" value="GRX_GRXh_1_2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000093">
        <property type="entry name" value="Glutaredoxin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000190">
        <property type="entry name" value="Thioredoxin reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.390.30:FF:000004">
        <property type="entry name" value="Thioredoxin reductase 1, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011767">
        <property type="entry name" value="GLR_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002109">
        <property type="entry name" value="Glutaredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011899">
        <property type="entry name" value="Glutaredoxin_euk/vir"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046952">
        <property type="entry name" value="GSHR/TRXR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012999">
        <property type="entry name" value="Pyr_OxRdtase_I_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006338">
        <property type="entry name" value="Thioredoxin/glutathione_Rdtase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02180">
        <property type="entry name" value="GRX_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01438">
        <property type="entry name" value="TGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42737">
        <property type="entry name" value="GLUTATHIONE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42737:SF8">
        <property type="entry name" value="THIOREDOXIN-DISULFIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="FAD/NAD-linked reductases, dimerisation (C-terminal) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00195">
        <property type="entry name" value="GLUTAREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00076">
        <property type="entry name" value="PYRIDINE_REDOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7 14" id="KW-0274">FAD</keyword>
    <keyword evidence="6 14" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0521">NADP</keyword>
    <keyword evidence="11 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13 14" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0712">Selenocysteine</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Glutaredoxin" evidence="15">
        <location>
            <begin position="20"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="17">
        <location>
            <begin position="111"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="domain" description="Pyridine nucleotide-disulphide oxidoreductase dimerisation" evidence="16">
        <location>
            <begin position="467"/>
            <end position="579"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ8046370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="594" mass="65343" checksum="E5BF9FA96AB7F534" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CKM5</accession>
    <name>A0A9Q1CKM5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Homeobox protein aristaless</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_05433</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CKM5"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000679">
        <property type="entry name" value="Homeobox protein aristaless"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050649">
        <property type="entry name" value="Paired_Homeobox_TFs"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24329:SF543">
        <property type="entry name" value="FI01017P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24329">
        <property type="entry name" value="HOMEOBOX PROTEIN ARISTALESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 5 6" id="KW-0371">Homeobox</keyword>
    <keyword evidence="4 5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="8">
        <location>
            <begin position="117"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="5">
        <location>
            <begin position="119"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="69"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="178"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="69"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="79"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="178"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="191"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="248"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8046671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="367" mass="40688" checksum="8136502671F80742" modified="2023-09-13" version="1">MPANCVTVPSSYGFYRPLYFAGPRNSELSPTSSLSSTDGRSSVDANMTYYKQTLWQGNSSRLTGQLDESVSQPSLGVTPSSSLAPSSELSPYKPPHPTPQDTRDATVSEMETYPPRNRRRRLRTIYTAHQIRGLEQCFNQNKYPDIGTREALAMAIGMSEARVQVWFQNRRARLRRQEKSLKRKEMDQQEENNGQNKRVCQSDDVSGSDEASHIPFTAESTEAVETSPRDDEDQEEIDVVSISKESDSPVNMPTSRSPSSDPTITSNYSTSPLIKVPAIVPTSPPQVCTKPCCCPPVVIPPYTYFPQHMASSLALSYHRGLLANRATVPLPELSTTAPWIYNFQSRYTTAPFPFSIAGSGFTSVPLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CPS1</accession>
    <name>A0A9Q1CPS1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_01302</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CPS1"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8048831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="105" mass="12245" checksum="983AFBB267F5FEF4" modified="2023-09-13" version="1">MLVAALERVERQQRAQRLRQEFLQRRCLSYYLKSVESACYDHTPKALFRKHQSAIGGGVLWIRLQNNFYKTFKTIPRKPPPYIAIFTVLGLWLGGVMALRRMCSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CTA9</accession>
    <name>A0A9Q1CTA9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Immunoglobulin V-set domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_03997</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CTA9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040436216" description="Immunoglobulin V-set domain-containing protein" evidence="3">
        <location>
            <begin position="25"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="160"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="203"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="215"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8050695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="274" mass="30385" checksum="42BEADB0ACDA49C6" modified="2023-09-13" version="1">MENARLHFVAFLTTFSLLNGYCLSAGVFCNSPQNGTIGAVGILQCSFESVFHTISWSYVGEDTHPLVRYDGGVLQGSGHNTDSYSLRIDGALLIIDAASRTGSKEYKVETIDDDGEYSSQTVEYQFKGESTIMHHTNAKSQTTHLVTDTPSFECPPRLNWYVLLGAFVGATVGAFVTGMIAYIKHRKHWCRYNVITLPSEFPRGGWKVGGRRKRADREIPVREIPPEQSIQRAKNMGASTKTKPDNEENNEETTPLNDNPSSKRKPATEGNIEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CU27</accession>
    <name>A0A9Q1CU27_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BRCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_03862</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="KAJ8050599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome 1"/>
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        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1347"/>
            <end position="1441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="370"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="493"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="384"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="540"/>
            <end position="558"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8050599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1528" mass="173155" checksum="1C7C81EA15460D45" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H0G2</accession>
    <name>A0A9Q1H0G2_HOLLE</name>
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        <submittedName>
            <fullName evidence="9">Dual specificity protein kinase Ttk</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_28473</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="20524at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:nucleus"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="F:protein serine/threonine kinase activity"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="PKc_Mps1"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="serine/threonine-protein kinase mph1 isoform X1"/>
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    </dbReference>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
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    </dbReference>
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        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="Mps1_cat"/>
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        <property type="entry name" value="Prot_kinase_dom"/>
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        <property type="entry name" value="Protein_kinase_ATP_BS"/>
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        <property type="entry name" value="Ser/Thr_kinase_AS"/>
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    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22974:SF21">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN KINASE TTK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22974">
        <property type="entry name" value="MIXED LINEAGE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="TPR-like"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="4 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="501"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="218"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="458"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="816"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="218"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="269"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="328"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="351"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="405"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="424"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="458"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
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    </feature>
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        <location>
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        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="530"/>
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        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8029144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="854" mass="94512" checksum="14ABC4C41230C8F6" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H3T4</accession>
    <name>A0A9Q1H3T4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="1" type="ORF">HOLleu_25883</name>
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    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
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            <taxon>Aspidochirotacea</taxon>
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            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome 12"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8032369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="51" mass="6004" checksum="6EECB8FDDE338B0B" modified="2023-09-13" version="1">MSEFSVNILRKFRSDEIGSTCRTDPVILNVGKRLWDKGRAKERKKKPEVRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H3X0</accession>
    <name>A0A9Q1H3X0_HOLLE</name>
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        <recommendedName>
            <fullName evidence="3">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="4" type="ORF">HOLleu_28075</name>
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        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
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            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Znf_CCHC_sf"/>
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    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="216"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="282"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8031366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="441" mass="50342" checksum="1ADA3A8B3EEF4F75" modified="2023-09-13" version="1">MEEEQLIPSFPDPTQEDMEFQTVTRKRRRTSNSAPGPEDQREPPRHHCKYPVILTGLPATMKNPIAIRKYIDANHPTAQVIRTTTSRTGHTIIAAQDETSQKTLLKDWKPIEGRRPTARLPKQKTARPETYEGVIIGLHPDITEEDATKEMGKTNGFDVISFRRFYRKGIRIPTWKAAVTFASNEDFQRALDKGVNIGYQHHRVEAIRREATPTQCHNCQRYGHLASNCSHRKTCLRCAGHHSLAECPNPRKQPKCANCSRSQIASYRGCSRYARALEELKKKTTKKTPQAQGNPRVPTARPDKPNPTSFCSPQIADLQKKHEDAIKKMEERHQLELEAIRLQHQATVEKIEEANTQLFHQIREDTTTQLNEMKCRIIYFLGDVLHHLIPTEEDKPPTDYQKATVTQQKAWAHLGLAIDISNIKAELKKKSSPPKAKKTSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H3Z4</accession>
    <name>A0A9Q1H3Z4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_24888</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H3Z4"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8031635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="131" mass="14500" checksum="B43A9123BDF0BEA7" modified="2023-09-13" version="1">MGLLATVAALGAIVGPYIPGITIVTAPAIPLWGPIAAVAGIMATFTGGLGLWINDKMEGSLLSTYMQRQAAVERKEFEEFQRNVIAGGFFIFLVIAVITVIFYIRKRRTKNQAQDGRETGWFRLRIPFIRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H490</accession>
    <name>A0A9Q1H490_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Tyrosine-protein kinase receptor Tie-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_25042</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H490"/>
    <dbReference type="OrthoDB" id="6406468at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.300.10">
        <property type="entry name" value="Tie2 ligand-binding domain superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038081">
        <property type="entry name" value="CalX-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052108">
        <property type="entry name" value="MEGF/SIB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24035">
        <property type="entry name" value="MULTIPLE EPIDERMAL GROWTH FACTOR-LIKE DOMAINS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24035:SF109">
        <property type="entry name" value="PROTEIN DRAPER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141072">
        <property type="entry name" value="CalX-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040421205" evidence="2">
        <location>
            <begin position="32"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="523"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="529"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="548"/>
            <end position="557"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8031741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="669" mass="74070" checksum="6FA3818EA545DB1F" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H5R3</accession>
    <name>A0A9Q1H5R3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Uridylate-specific endoribonuclease</fullName>
            <ecNumber evidence="12">4.6.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HOLleu_24220</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Nanhai2018</strain>
            <tissue evidence="15">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ribonucleotidyl-uridine-RNA = a 5'-end dephospho-uridine-RNA + a 3'-end 2',3'-cyclophospho-ribonucleotide-RNA</text>
            <dbReference type="Rhea" id="RHEA:67792"/>
            <dbReference type="Rhea" id="RHEA-COMP:10464"/>
            <dbReference type="Rhea" id="RHEA-COMP:17354"/>
            <dbReference type="Rhea" id="RHEA-COMP:17356"/>
            <dbReference type="ChEBI" id="CHEBI:83064"/>
            <dbReference type="ChEBI" id="CHEBI:173117"/>
            <dbReference type="ChEBI" id="CHEBI:173224"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3 12">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the ENDOU family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.-" evidence="12"/>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H5R3"/>
    <dbReference type="OrthoDB" id="430326at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd21159">
        <property type="entry name" value="XendoU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039787">
        <property type="entry name" value="ENDOU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037227">
        <property type="entry name" value="EndoU-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018998">
        <property type="entry name" value="EndoU_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036024">
        <property type="entry name" value="Somatomedin_B-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001212">
        <property type="entry name" value="Somatomedin_B_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12439:SF42">
        <property type="entry name" value="ENDORIBONUCLEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12439">
        <property type="entry name" value="PLACENTAL PROTEIN 11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01033">
        <property type="entry name" value="Somatomedin_B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09412">
        <property type="entry name" value="XendoU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00201">
        <property type="entry name" value="SO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142877">
        <property type="entry name" value="EndoU-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90188">
        <property type="entry name" value="Somatomedin B domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51959">
        <property type="entry name" value="ENDOU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50958">
        <property type="entry name" value="SMB_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 12" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0456">Lyase</keyword>
    <keyword evidence="10 12" id="KW-0464">Manganese</keyword>
    <keyword evidence="5 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 12" id="KW-0540">Nuclease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040540290" description="Uridylate-specific endoribonuclease" evidence="12">
        <location>
            <begin position="20"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="SMB" evidence="13">
        <location>
            <begin position="20"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="SMB" evidence="13">
        <location>
            <begin position="63"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="EndoU" evidence="14">
        <location>
            <begin position="111"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU367085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ8033853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="371" mass="41276" checksum="042420F7AB49DD75" modified="2023-09-13" version="1">MKQVIALLLCACILRSAVGNTSLCANRCLIDYDASLPCQCNDVCGNYGNCCEDYYTVGCNTASTSRCEDRCLVGYDSSLPCQCNDQCKSFGNCCSDFDAVDCAAVHSGETGSMTLAEFAEDLWANDENRVASSRYTYNRGTYISNTNDKTDVSLSNFFSYFDSSILNGATISKFYALLNNYIATEGNTESFTSTENNEITAFLDAIFSTTVMQKTVTYLINEGLVSSESDLRNKMDTMWFEPYNRQGTDDSSGFEHVFLGEWKSSTTVNGFHNWIQFYKEENEGDINYFGYVEEANPNLISMQFSWGSSSRVKKLTSIMLGASPEFEMAMFTTCFLKHKGSLCTFTLDGHTVQIQTYSQNTDQVGSAYFAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H736</accession>
    <name>A0A9Q1H736_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_22349</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H736"/>
    <dbReference type="OrthoDB" id="5984724at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331:SF1">
        <property type="entry name" value="GAG-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8035203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="95" mass="10890" checksum="0B0FCB53A672E72A" modified="2023-09-13" version="1">MADLPKKRFEDSKPPFSYVGIDVFGPFYVKLGGSEVIRYGCLFNCLSIRAIHVEKLDKLDTNTFINAFRNFISRRDHPEVVFSYKGSNLVGLKVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H755</accession>
    <name>A0A9Q1H755_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Fibrinogen-like protein A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_19396</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8035654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H755"/>
    <dbReference type="OrthoDB" id="7735550at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037579">
        <property type="entry name" value="FIB_ANG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040941">
        <property type="entry name" value="GGGWT_bact"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47221">
        <property type="entry name" value="FIBRINOGEN ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47221:SF6">
        <property type="entry name" value="FIBRINOGEN ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00186">
        <property type="entry name" value="FBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040375380" evidence="7">
        <location>
            <begin position="25"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="8">
        <location>
            <begin position="34"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="8">
        <location>
            <begin position="185"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8035654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="297" mass="33942" checksum="5410B15419C327DC" modified="2023-09-13" version="1">MRYITSLQLISILLSSLLLDDVHSSTSGSSYFFYQTPVYPRDCQEVFQQCSKNNASGVYLIKPDGYDEAFEVFCDNQVDGGGWTVFQRRNEDNTVEFTRNFSEYRKGFGFLRREFWLGNERLSFLTNQKRYQLRIDMTNSTGSYFYLTYDLFRISDEWGNYKLTSVGQYNGTADTFVGQEPVSTPGAPSTPTDCYDIFTSGFSDGVYTIQPTGWTGSPFEVYCNMSHGGGWTVLQRRVDGSLSFDRNWDAYKAGFGSAGPDENVWLGNEVLYHMTSQKNYALRIDVVKPDLGDWHMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H7Q4</accession>
    <name>A0A9Q1H7Q4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">CDC42 small effector protein 2-A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_19303</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CDC42SE/SPEC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8035580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H7Q4"/>
    <dbReference type="OrthoDB" id="5559822at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035023">
        <property type="term" value="P:regulation of Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00132">
        <property type="entry name" value="CRIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.810.10:FF:000004">
        <property type="entry name" value="CDC42 small effector protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.810.10">
        <property type="entry name" value="CRIB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000095">
        <property type="entry name" value="CRIB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036936">
        <property type="entry name" value="CRIB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039056">
        <property type="entry name" value="SPEC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13502">
        <property type="entry name" value="CDC42 SMALL EFFECTOR PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13502:SF6">
        <property type="entry name" value="CDC42 SMALL EFFECTOR PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00786">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50108">
        <property type="entry name" value="CRIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0133">Cell shape</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0564">Palmitate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRIB" evidence="12">
        <location>
            <begin position="31"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="45"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="47"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8035580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="93" mass="10237" checksum="744EB5F831E1ABE4" modified="2023-09-13" version="1">MSDMLLCFSCCCTEPAAAPIRTRRKIDRSMIGTPQNFVHTAHIGSGDMTTAQNSHISSIQTQMSSKGGYEPNGTSPVQFERKVIDVQRTDTEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H902</accession>
    <name>A0A9Q1H902_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">LRAT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_17792</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H902"/>
    <dbReference type="OrthoDB" id="421951at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1720.10">
        <property type="entry name" value="endopeptidase domain like (from Nostoc punctiforme)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007053">
        <property type="entry name" value="LRAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04970">
        <property type="entry name" value="LRAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="122"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="154"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="208"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="246"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="LRAT" evidence="3">
        <location>
            <begin position="61"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="281"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="368"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="281"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="318"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="372"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="416"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8037065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="433" mass="49180" checksum="6DA3C6ED4415A269" modified="2023-09-13" version="1">MWRGSGPLLIGTTPLLPRMFKKMRMSSMLLSGFQKKICKNTKKKFDLLGNCCYSPMYKFVYPKEIEEQNPPDLVMLRASTMMSLDIRYSLFVDNNEHFATFCKTGSHKSNQLFQYSVRMHAWIARILVAFLHIAFVVSFSEMFEWFVPHIDEDWLGFTIHCVFECLTFLMIIFITYKIDTPKQNFKRSNQSGCTLACKRAVIKSLMQSVTLIGFVTLFGVFVVRLVAPKNGKKWSVKKRVGIEMGLGFVGGVIGNMIGFIFCHFIPYPCCRTVIREEDAGTETKHVEEGKEGHPQSQSTGSTPVPKRSAIASDISETTYHDQGRLSGRKEDAKDEKVIFKKSPDDILQDDEIRETTISGSKIKLNIDGDTSYVRRETPKSSREGEEGESETDSTQFKIVEESFPSSTEDVSPETEAEIHESKQDETNLDDADM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H9F2</accession>
    <name>A0A9Q1H9F2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nucleolar protein 12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_18826</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRP17 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H9F2"/>
    <dbReference type="OrthoDB" id="10072223at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019186">
        <property type="entry name" value="Nucleolar_protein_12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14577">
        <property type="entry name" value="NUCLEOLAR PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14577:SF0">
        <property type="entry name" value="NUCLEOLAR PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09805">
        <property type="entry name" value="Nop25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="85"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="45"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="85"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="100"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="151"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="174"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="199"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8037889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="220" mass="26369" checksum="D3B1D40666EF8239" modified="2023-09-13" version="1">MRDRHRQKNPKNRKTKVHLVFDEESRKEFLTGFRRRKLDRKKFHQKRLAEQHKEAVRQMRRERKKALEESVNLNLQFPDEKLEKELSSQNQEVHDLPDHTVTVTSVTDLDLSSTPGGLGPPELEYESDESEDTIHKEDDEEDEDDFGFPLTHREMKVEKEPPEIPNEQTENIPQKRKRVFHKQKSSKKRPRKVGTPSNLHRRKRKRLATSRKHINYTKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HB36</accession>
    <name>A0A9Q1HB36_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Golgi-associated plant pathogenesis-related protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_17330</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HB36"/>
    <dbReference type="OrthoDB" id="337038at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05382">
        <property type="entry name" value="CAP_GAPR1-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.33.10:FF:000002">
        <property type="entry name" value="Golgi-associated plant pathogenesis-related protein 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018244">
        <property type="entry name" value="Allrgn_V5/Tpx1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001283">
        <property type="entry name" value="CRISP-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034113">
        <property type="entry name" value="SCP_GAPR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002413">
        <property type="entry name" value="V5_allergen-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10334">
        <property type="entry name" value="CYSTEINE-RICH SECRETORY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00838">
        <property type="entry name" value="V5ALLERGEN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00837">
        <property type="entry name" value="V5TPXLIKE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00198">
        <property type="entry name" value="SCP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="PR-1-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01009">
        <property type="entry name" value="CRISP_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCP" evidence="2">
        <location>
            <begin position="12"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="SCP" evidence="2">
        <location>
            <begin position="220"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="domain" description="SCP" evidence="2">
        <location>
            <begin position="422"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="156"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="177"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="202"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8039569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="566" mass="64033" checksum="B1776DB64D33C609" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HBE5</accession>
    <name>A0A9Q1HBE5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_16947</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HBE5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8039283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="76" mass="8947" checksum="5FA69BAAE14A1721" modified="2023-09-13" version="1">MESSREFNFNAAFLELSRSENCLRYVPITTTVCSRSYYLVVIHSWNQFLRYSTLQVYYCTIVKLHALPALGPDQKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HFZ2</accession>
    <name>A0A9Q1HFZ2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Heparan sulfate glucosamine 3-O-sulfotransferase 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_10799</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HFZ2"/>
    <dbReference type="OrthoDB" id="16988at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008467">
        <property type="term" value="F:[heparan sulfate]-glucosamine 3-sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037359">
        <property type="entry name" value="NST/OST"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10605:SF65">
        <property type="entry name" value="GH20068P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10605">
        <property type="entry name" value="HEPARAN SULFATE SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sulfotransferase" evidence="7">
        <location>
            <begin position="101"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="42"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="active site" description="For sulfotransferase activity" evidence="3">
        <location>
            <position position="109"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="330"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR637359-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR637359-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR637359-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8043621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="383" mass="43935" checksum="EABCF658E3E3655B" modified="2023-09-13" version="1">MEQNTKLKQNSTEFGGSRYILQNKQSSEEAPVQSGYISSTNHSDKKKEVKKESDNHSKAANVSKELLWGCYEYKKGSLSLARKKASENILKKMNCSRKLPEAWIIGVKKCGSQTLTGLLDLHPEIVTNLYLNDKNSMFLNETEFKDTYSPWTRPDEMGLFSMVGLQNRPDVLGHVVRNLATPATRYLLILVDPVKRALSDYVHCTKIMKKPKFVHSFVTERRLVWDGKLQNYSHENVSLFKDTKIASTFERSVFNLSGELDIESGLIKRGVYSNSVEEILKLVSRDKLLVIDGQKFVTQPWEVLSQTESFLNVSAFFSRQHFLQQGSFFCPLVKERPDADCIPGKGNKKPEVSPNVLRELKNFYRPYNRKLQILLGEKYSWMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HHY7</accession>
    <name>A0A9Q1HHY7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ankyrin repeat and BTB/POZ domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_06298</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HHY7"/>
    <dbReference type="OrthoDB" id="684045at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18497">
        <property type="entry name" value="BACK_ABTB1_BPOZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18296">
        <property type="entry name" value="BTB2_POZ_ABTB1_BPOZ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044515">
        <property type="entry name" value="ABTB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46231">
        <property type="entry name" value="ANKYRIN REPEAT AND BTB/POZ DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46231:SF1">
        <property type="entry name" value="ANKYRIN REPEAT AND BTB_POZ DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BTB" evidence="3">
        <location>
            <begin position="98"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="3">
        <location>
            <begin position="254"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8047324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="459" mass="52286" checksum="AF0023AE7BDEB68F" modified="2023-09-13" version="1">MRVCMKFFNPAKFLGTENILEMQSVYYSKLDHCLAMCHNSPGAKCVAKTFDGERCLYGALTDEIRDILKNYKVVDVGHGRKNLYQEFLRQLLESGENRDIEFVVHHESIPAHRCILHARSPYFAEMLCTKWANRSVVHIKSSVSVDRFIEIFPSGYLQVSLDCVDDCLRFAKQCQMTTLMEEINKRLEVIEKFVPQKPGTEISVLSVEPDPNSSTLQDDLDELAELAIPPPLRRLVDIDDIPFYGEVEDAPPYADICFSVEGHSFYCHQIFFKRRSDYFKALITDHFGEAGEDPSSVPVITLHDISAKIFCKVVYYLYTGMVENLNDVSAYELLCNADLFLLVGLKKQCANEIANHLTVENVINVLRVGRMFNLSKLEDQCAEFMANNLEQLINTPEFSDIIKEDAASIQQRQETDSIMIIDDIRCHISSSVQTFSAVQEAQEKLAELDELLIRLGLEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HI39</accession>
    <name>A0A9Q1HI39_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_04221</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HI39"/>
    <dbReference type="OrthoDB" id="775972at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon gypsy-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF15">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="1">
        <location>
            <begin position="54"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8050862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="209" mass="24633" checksum="3ABEF6483126C35B" modified="2023-09-13" version="1">MLSHDRAGHMGVEKTKDRILQHFYWPNMFQDVMRYCRSCEICQKMDKSGKKRKYPLLPMRIIGVLFKRIGIDIVGPLIRSRKKKMHLPTICDFPTTYPEAIPLANIRTNTVVDALIHVFARIVLPQEMLHDQRTNFMSKVMKLFCQKLGISQSATSARHQQTNVFTERFHGTLKNMLRSLSDEQMKHWDEYIPYFLFSYREVPCQTTGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HIC7</accession>
    <name>A0A9Q1HIC7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_06885</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HIC7"/>
    <dbReference type="OrthoDB" id="5949854at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47018">
        <property type="entry name" value="CXC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47018:SF2">
        <property type="entry name" value="TESMIN_TSO1-LIKE CXC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8047789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="879" mass="99467" checksum="29766916249BF606" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HIN7</accession>
    <name>A0A9Q1HIN7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_04893</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HIN7"/>
    <dbReference type="OrthoDB" id="10037236at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8046273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="88" mass="9980" checksum="3A32C50D34045D39" modified="2023-09-13" version="1">MDELCGSVKYLSYFRNASILSFTETWLTDNHTDDCVSVDGFKIIRGDRDLEAAGKRSGGGVCVYININCCHPNNAYRKDYLCNPMWKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HK17</accession>
    <name>A0A9Q1HK17_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_01042</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HK17"/>
    <dbReference type="OrthoDB" id="5376140at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33480">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33480:SF1">
        <property type="entry name" value="TYR RECOMBINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="193"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="210"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="233"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8048645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="253" mass="28859" checksum="752B812CF3B75C1B" modified="2023-09-13" version="1">MHWFLLSSKPKRNTGNINCDVLQSLTTWEQTLCNKLIRIEVKGKKGRKVPVLLTGEMTESLELLMKHRNSVCIRSDNVFVFARANGDSQMCIRGSDCLRKLAIKSCVKIPANLTSTKLRKQIATVSQIFCLRDNELDILAGFLGHDIRVHRDYYRLPEDALQTAKVARLLMLMEKGLVADFKDKTLSEIEVALDGPSYDDVDDGSGKEESEVEEDEEEEEDKEDVNGDEEEKDVERTEQQSEERREGGYQWGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-04-02" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HK94</accession>
    <name>A0A9Q1HK94_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Plant heme peroxidase family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_00334</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10575391at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005261">
        <property type="term" value="F:monoatomic cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006816">
        <property type="term" value="P:calcium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002016">
        <property type="entry name" value="Haem_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002859">
        <property type="entry name" value="PKD/REJ-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46730:SF1">
        <property type="entry name" value="PLAT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46730">
        <property type="entry name" value="POLYCYSTIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02010">
        <property type="entry name" value="REJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50873">
        <property type="entry name" value="PEROXIDASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040413953" description="Plant heme peroxidase family profile domain-containing protein" evidence="8">
        <location>
            <begin position="27"/>
            <end position="1801"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1765"/>
            <end position="1788"/>
        </location>
    </feature>
    <feature type="domain" description="Plant heme peroxidase family profile" evidence="9">
        <location>
            <begin position="921"/>
            <end position="1182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="383"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="383"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="396"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="424"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="447"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="483"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="497"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="564"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="596"/>
            <end position="614"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8048148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1801" mass="197681" checksum="3BB46BDAD63CC653" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HL45</accession>
    <name>A0A9Q1HL45_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">B box-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_01825</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HL45"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19756">
        <property type="entry name" value="Bbox2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047153">
        <property type="entry name" value="TRIM45/56/19-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25462">
        <property type="entry name" value="BONUS, ISOFORM C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25462:SF296">
        <property type="entry name" value="MEIOTIC P26, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="B box-type" evidence="3">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="220"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="228"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="263"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="277"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="300"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8049198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="655" mass="73557" checksum="FD0223002210E4F1" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HLJ6</accession>
    <name>A0A9Q1HLJ6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Gamma-glutamylcyclotransferase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_02682</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the gamma-glutamylcyclotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HLJ6"/>
    <dbReference type="OrthoDB" id="113620at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061929">
        <property type="term" value="F:gamma-glutamylaminecyclotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06661">
        <property type="entry name" value="GGCT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.490.10">
        <property type="entry name" value="Gamma-glutamyl cyclotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009288">
        <property type="entry name" value="AIG2-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039126">
        <property type="entry name" value="GGACT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013024">
        <property type="entry name" value="GGCT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036568">
        <property type="entry name" value="GGCT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12510:SF4">
        <property type="entry name" value="GAMMA-GLUTAMYLAMINECYCLOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12510">
        <property type="entry name" value="TROPONIN C-AKIN-1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06094">
        <property type="entry name" value="GGACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110857">
        <property type="entry name" value="Gamma-glutamyl cyclotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gamma-glutamylcyclotransferase AIG2-like" evidence="4">
        <location>
            <begin position="24"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR639126-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU367036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF06094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8049783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="161" mass="18432" checksum="14221DA3C18677CE" modified="2023-09-13" version="1">MKGREFEPFSTLQIFSAALVMYRIFIYGTLKANEMNSFHMTDDSHGKTVFVGKGKTSERYPLVLVAFKQFRNIPGMIYQEGVGHHIDGEIWDVDETFLKWLDNFEGYPDVYRRETLKITRADGSGEDTCLVYIKCNVQISESSNLLTSYNSAECGRPYIPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0Y9N8</accession>
    <name>A0A9Q0Y9N8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">DNA topoisomerase 2</fullName>
            <ecNumber evidence="13">5.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HOLleu_43253</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Nanhai2018</strain>
            <tissue evidence="16">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12 13">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="13">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the type II topoisomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="13"/>
    <dbReference type="EMBL" id="JAIZAY010000246">
        <property type="protein sequence ID" value="KAJ8018653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0Y9N8"/>
    <dbReference type="OrthoDB" id="276498at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003918">
        <property type="term" value="F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000712">
        <property type="term" value="P:resolution of meiotic recombination intermediates"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000819">
        <property type="term" value="P:sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03481">
        <property type="entry name" value="TopoIIA_Trans_ScTopoIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03365">
        <property type="entry name" value="TOPRIM_TopoIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.40:FF:000003">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.30:FF:000001">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000008">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.670:FF:000001">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.199.10:FF:000002">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.670">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.199.10">
        <property type="entry name" value="Topoisomerase II, domain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050634">
        <property type="entry name" value="DNA_Topoisomerase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001241">
        <property type="entry name" value="Topo_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013760">
        <property type="entry name" value="Topo_IIA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013758">
        <property type="entry name" value="Topo_IIA_A/C_ab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013759">
        <property type="entry name" value="Topo_IIA_B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013506">
        <property type="entry name" value="Topo_IIA_bsu_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002205">
        <property type="entry name" value="Topo_IIA_dom_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001154">
        <property type="entry name" value="TopoII_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018522">
        <property type="entry name" value="TopoIIA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031660">
        <property type="entry name" value="TOPRIM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034157">
        <property type="entry name" value="TOPRIM_TopoII"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10169:SF38">
        <property type="entry name" value="DNA TOPOISOMERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10169">
        <property type="entry name" value="DNA TOPOISOMERASE/GYRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00204">
        <property type="entry name" value="DNA_gyraseB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00521">
        <property type="entry name" value="DNA_topoisoIV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01751">
        <property type="entry name" value="Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16898">
        <property type="entry name" value="TOPRIM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01158">
        <property type="entry name" value="TOPISMRASEII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00418">
        <property type="entry name" value="TPI2FAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00433">
        <property type="entry name" value="TOP2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00434">
        <property type="entry name" value="TOP4c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56719">
        <property type="entry name" value="Type II DNA topoisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52040">
        <property type="entry name" value="TOPO_IIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00177">
        <property type="entry name" value="TOPOISOMERASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 12 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11 12 13" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12 13" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="Toprim" evidence="14">
        <location>
            <begin position="354"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="domain" description="Topo IIA-type catalytic" evidence="15">
        <location>
            <begin position="614"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="active site" description="O-(5'-phospho-DNA)-tyrosine intermediate" evidence="12">
        <location>
            <position position="704"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU362094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS52040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ8018653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="908" mass="103398" checksum="2B2105973DAFB1FA" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YA69</accession>
    <name>A0A9Q0YA69_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">KIF1-binding protein-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_45118</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010001880">
        <property type="protein sequence ID" value="KAJ8017460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YA69"/>
    <dbReference type="OrthoDB" id="409897at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021952">
        <property type="term" value="P:central nervous system projection neuron axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990535">
        <property type="term" value="P:neuron projection maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46321:SF1">
        <property type="entry name" value="KIF-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46321">
        <property type="entry name" value="KIF1-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8017460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="104" mass="12159" checksum="917B11DC5971F173" modified="2023-09-13" version="1">MSASLQYHLGLNYAETEEISSGEEHLVRCNEKLEDRRLDHRCVALAISTLNQLGIIWCQRSETKKSLEFLKVKNLFFIIGSIMCPLHNLQELCSRLYFQNDERK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YAI3</accession>
    <name>A0A9Q0YAI3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Pygopus-like 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_44377</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000832">
        <property type="protein sequence ID" value="KAJ8017921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YAI3"/>
    <dbReference type="OrthoDB" id="6143588at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="5">
        <location>
            <begin position="106"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8017921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="296" mass="33360" checksum="EFF46650DC914B23" modified="2023-09-13" version="1">MVFKSYLLILPGILAFTCWRILNPVHTQLEKDQLPPFLEGHWTAPLIIHNKLFTPLNPVSSFHFSIQCRHKLLKFPKTKVPAHVLLLTTLLAGDIESNPGPGRRPKFPCQICHKAAKWGQKAIECDKCQGWFHVTCLNMNEMIYNVLSEHPSYSWSCCNCGLPNFSSTFMESLLDLELSNSFSLLSHDSVEHSIHVMSQNPGVPLSASSPKIDRRNVRPKLNPSSNLRTLSAELSRRRLPTNLKKLKIINVNCQSIRAKLPSFEVMLATEDPDIVIGTESWLNDSIATGEVFPKLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YBG1</accession>
    <name>A0A9Q0YBG1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_43878</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000513">
        <property type="protein sequence ID" value="KAJ8018240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YBG1"/>
    <dbReference type="OrthoDB" id="10063525at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="33"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="133"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="49"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8018240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="167" mass="20398" checksum="CF104E9BEA7AA840" modified="2023-09-13" version="1">MRIKTTRSNEQYRKREIKANCNRMKTIRSAEEYRKKESQANLNRMKTIRSNEEYKRKESEVNLNRVKTNRSNEEYKKKESQANLNRMKTVRSNEEYKKKESQANLNRMKTIRSDEEYKRKESQANLNRIKTIRSDEEYKKKESQANLNRMKQQLELMREYKREGKSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YBW7</accession>
    <name>A0A9Q0YBW7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_41559</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8019813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10025024at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01644">
        <property type="entry name" value="RT_pepA17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005312">
        <property type="entry name" value="DUF1759"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040676">
        <property type="entry name" value="DUF5641"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008042">
        <property type="entry name" value="Retrotrans_Pao"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331:SF5">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03564">
        <property type="entry name" value="DUF1759"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18701">
        <property type="entry name" value="DUF5641"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05380">
        <property type="entry name" value="Peptidase_A17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="3">
        <location>
            <begin position="1643"/>
            <end position="1835"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="232"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="149"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8019813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1969" mass="222998" checksum="6E232CD1639D5757" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YBW9</accession>
    <name>A0A9Q0YBW9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">SOSS complex subunit B1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_41750</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8019958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YBW9"/>
    <dbReference type="OrthoDB" id="295715at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070876">
        <property type="term" value="C:SOSS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044818">
        <property type="term" value="P:mitotic G2/M transition checkpoint"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010212">
        <property type="term" value="P:response to ionizing radiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04491">
        <property type="entry name" value="SoSSB_OBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000072">
        <property type="entry name" value="SOSS complex subunit B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051231">
        <property type="entry name" value="SOSS-B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13356">
        <property type="entry name" value="OB FOLD NUCLEIC ACID BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13356:SF0">
        <property type="entry name" value="SOSS COMPLEX SUBUNIT B HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="108"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="137"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8019958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="204" mass="22214" checksum="B3ADFE5783347953" modified="2023-09-13" version="1">MLCPDFAEVMVEGSATKTKEGHTVWTVRVADKTASINLSVWDALGQALQAGDIIRLQKGYVSLYRGILTLYCGSVGKLFKMGEFCFVFSEFPNMSEANPEFMAQMKQPNFLSNNSNPPPQLPGQSLEGRAPGHPPLNGNNGARHNFPVQNQYSQPPPPLPGQDFQQGAYPTPNWNIAGKPSDGRGRGRGGGGQQDPRIKSKRGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YEY7</accession>
    <name>A0A9Q0YEY7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_40239</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YEY7"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.4.10">
        <property type="entry name" value="Hepatocyte Growth Factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00024">
        <property type="entry name" value="PAN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sushi" evidence="3">
        <location>
            <begin position="27"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="2">
        <location>
            <begin position="168"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8020604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="323" mass="35693" checksum="EDBB297A2714C562" modified="2023-09-13" version="1">MQPFLCTHYADGIHSVDELTVTCSSDDLPDVINGSFSCSNPVNVGDVCNLTCIENYYPSLLSQTTCMNVGTEVANWSNVTELLCSVNTCSFEDLPDVTSGVFDQSKCSGQNIDAVCNVSCSPGLYPDSSDATVCRSVDGKKAEWMVKNVYECKDYWSETMMACIRDHNDLEITNTDNIEVCKDLCMQEHSITCLSVDFISNRCYMSSRNRHNIELADDFVVPCYIIGAVYAERIDIASWSSEVNTCLDGLGNLWSGSLIDLDSCKLICSQTIDCCSLMFKNGLCHLSKSQKDILPASFPNDTTPSDGCNWVYTERIDKTCPIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YFD3</accession>
    <name>A0A9Q0YFD3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_42519</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000083">
        <property type="protein sequence ID" value="KAJ8019107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YFD3"/>
    <dbReference type="OrthoDB" id="161570at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd20104">
        <property type="entry name" value="MBT_PHF20L1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8019107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="68" mass="7930" checksum="0A3BDE9EDD25199E" modified="2023-09-13" version="1">MEEFKVGQKVQALNELARWESAKVLAINEEENRLLVNFAGWGPEFDEWMHTKRVRLPVLGFQSEIGKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YFQ7</accession>
    <name>A0A9Q0YFQ7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_42159</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000036">
        <property type="protein sequence ID" value="KAJ8019317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YFQ7"/>
    <dbReference type="OrthoDB" id="2160826at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33206:SF1">
        <property type="entry name" value="DNA-DIRECTED DNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33206">
        <property type="entry name" value="PROTEIN CBG10425"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="135"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8019317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="324" mass="37290" checksum="3EA055795E53A36B" modified="2023-09-13" version="1">MSNNTKTHEIFKDNLCLFRCLAHHLKTNLGICNERPTDPFAKWQAANGGRASVSTFKGVSFQELGDIEKLFKCNIYMYNLKPEEVDVSSSNSTSPKLVCELLRRSPSRYDSTMCLNYYKGHFYFITDMNYYSKSFSCSRCRRLFTKHKNLQKHIPHCKQHVTYQFPGGVFELPPTVFDKLRAVGITVDKEEQFYPYRITYDIETSLDKVDFKQCNAQKLNFIGQHKMLSISVCSNVPGITDPKCFISQGDSAQLVDNFVNYCNNISQSAFEIISNRNSMRSAFEDLHSMIKDKERENGEQDSESECQLLSRVFKPLLGSLTNYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YGD5</accession>
    <name>A0A9Q0YGD5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_39661</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8022237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YGD5"/>
    <dbReference type="OrthoDB" id="10059338at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33480">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33480:SF1">
        <property type="entry name" value="TYR RECOMBINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8022237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="279" mass="31100" checksum="45D3EEADF3CF0B1D" modified="2023-09-13" version="1">MKEDIKTHGVTSSNHGQLCQATFAQVILFNRRRPGETQYLKLTTFQNNLVRTTDAGDDIIQSLSISEKVAMDRLSLLYTRGKRDRGVPIMLADDLKESIEVLCENRKEAGVHPDNIYVSARCGSSLQPFRGCDVMKKFASDCGAKNPENLTSTKLRKHLATMSQIINLKDNELEQLANHLGHDVAVHREYYRLPQESLLLAKVSKLLFAMEAGKLHEYRGMNLDEITVSSLEEADLDEESLSDDCAALASSSERDQPSKVRVGGAPSDVPISRLTLHIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YH79</accession>
    <name>A0A9Q0YH79_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Tyramine/octopamine receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_39403</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8022029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YH79"/>
    <dbReference type="OrthoDB" id="6376512at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00637">
        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248">
        <property type="entry name" value="ADRENERGIC RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248:SF144">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="31"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="67"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="98"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="147"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="189"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="455"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="494"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="47"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="302"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="302"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="315"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="347"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="361"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8022029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="546" mass="60451" checksum="6A37B2DAFD99F241" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YHE1</accession>
    <name>A0A9Q0YHE1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mediator of RNA polymerase II transcription subunit 30</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Mediator complex subunit 30</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_40166</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Mediator complex subunit 30 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YHE1"/>
    <dbReference type="OrthoDB" id="10067025at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021019">
        <property type="entry name" value="Mediator_Med30_met"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31705">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31705:SF4">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11315">
        <property type="entry name" value="Med30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8020549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="118" mass="13475" checksum="88BB19D58D747CF2" modified="2023-09-13" version="1">MKFSVIFFFSQLPNGATVTVQAYKEKQDKLKEIVKGITTSFKSLRAVYDRCNQIAASLEHTNPNDYIPMVGDPDNWSDLISSGYKERAQHLGEEKKELIEVRSRDDDQICIDLFSPSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YHX7</accession>
    <name>A0A9Q0YHX7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">protein acetyllysine N-acetyltransferase</fullName>
            <ecNumber evidence="4">2.3.1.286</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_37331</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the sirtuin family. Class I subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.286" evidence="4"/>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YHX7"/>
    <dbReference type="OrthoDB" id="424302at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005637">
        <property type="term" value="C:nuclear inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033553">
        <property type="term" value="C:rDNA heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017136">
        <property type="term" value="F:histone deacetylase activity, NAD-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002039">
        <property type="term" value="F:p53 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01408">
        <property type="entry name" value="SIRT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1600.10:FF:000013">
        <property type="entry name" value="NAD-dependent protein deacetylase sirtuin-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1600.10">
        <property type="entry name" value="SIR2/SIRT2 'Small Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050134">
        <property type="entry name" value="NAD-dep_sirtuin_deacylases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003000">
        <property type="entry name" value="Sirtuin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026591">
        <property type="entry name" value="Sirtuin_cat_small_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026590">
        <property type="entry name" value="Ssirtuin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085:SF9">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACETYLASE SIRTUIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACYLASE SIRTUIN-5, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02146">
        <property type="entry name" value="SIR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50305">
        <property type="entry name" value="SIRTUIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Deacetylase sirtuin-type" evidence="12">
        <location>
            <begin position="234"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="512"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="579"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="645"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="757"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="850"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="39"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="126"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="138"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="512"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="533"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="579"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="655"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="692"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="785"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="850"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="361"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="369"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="372"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="393"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="396"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8022434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="892" mass="98161" checksum="A19AE9FB2F37FF2B" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YJA7</accession>
    <name>A0A9Q0YJA7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DDE Tnp4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_36048</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YJA7"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8023573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="118" mass="13302" checksum="B29BFA52B2B060C9" modified="2023-09-13" version="1">MAIRGFPHMAWAIDGIHVGLHGLICGCDHELFTLFQGFYNIYVQLVCDINYRTLDVMARQSCCTHRSRIFQESGLSHAYQNGILQSIFMGDSGYPKPWLLTPIPPTQTAAEETTTRLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YJN9</accession>
    <name>A0A9Q0YJN9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Death domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_36321</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YJN9"/>
    <dbReference type="OrthoDB" id="100767at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01670">
        <property type="entry name" value="Death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000488">
        <property type="entry name" value="Death_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00531">
        <property type="entry name" value="Death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00005">
        <property type="entry name" value="DEATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50017">
        <property type="entry name" value="DEATH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Death" evidence="1">
        <location>
            <begin position="80"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8023783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="149" mass="16950" checksum="C833FBE4F24324F6" modified="2023-09-13" version="1">MIAASMEKSSPFFLRRINIGTSYCVFQVSQASQASEIVELSLLLKPVGGVPAPTSRAEYNAMEDKMLDCFMFLKRNLVSDWKDVGRYLRVPEAELSRFSKESSIGESIFQMLLYWQRKNASEASTESLVKALKKLNRKDLIEEITEILN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YKJ1</accession>
    <name>A0A9Q0YKJ1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Sulfotransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_39229</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YKJ1"/>
    <dbReference type="OrthoDB" id="10019582at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053259">
        <property type="entry name" value="Golvesin-related_Golgi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32301">
        <property type="entry name" value="COUNTIN RECEPTOR CNR3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32301:SF6">
        <property type="entry name" value="GOLVESIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8021897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="371" mass="43315" checksum="9E15FC163837EFC2" modified="2023-09-13" version="1">MKQSISHQVGNFFMILCGVACFMMIFVFLDMEESYENTYQRIKVIERSQLQNLTAGKLITTRKRSNECSIVHPKLNSCNTLPNYYLYPERRDEFQSAVINFWHIQKAGGSSVERCLNEMQPKLNLPPAQRGGISIGQLEETYSKLSYFQNDSLKPVIQGHNTLGLCDFFHDINLGRKCSTFTLFREPYERAVSHYYFSRQVAIKGNPNKGYQRALKMPITEWVKIDGSPMWAAFYRLWRMDPTTNGRIKFQIISKNIPAQKWPSNDTIADEIIDNLDKHLSFVGLLEDMLTTYQMLEEIYNLPFVNECYGRHFNKGNYENLNATAKRELEKKAKRTLMEDKEIRKLMTFDVRLYEKAKEIFFAQMKLRAGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YLW7</accession>
    <name>A0A9Q0YLW7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Laminin-like protein epi-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_36425</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YLW7"/>
    <dbReference type="OrthoDB" id="7457896at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="612"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="649"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="828"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="651"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="702"/>
            <end position="714"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8023861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1105" mass="128578" checksum="FE7652723E069CEC" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YQ08</accession>
    <name>A0A9Q0YQ08_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ELL-associated factor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_34528</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EAF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YQ08"/>
    <dbReference type="OrthoDB" id="125903at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032783">
        <property type="term" value="C:super elongation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003711">
        <property type="term" value="F:transcription elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027093">
        <property type="entry name" value="EAF_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019194">
        <property type="entry name" value="Tscrpt_elong_fac_Eaf_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15970">
        <property type="entry name" value="ELL-ASSOCIATED FACTOR EAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15970:SF2">
        <property type="entry name" value="ELL-ASSOCIATED FACTOR EAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09816">
        <property type="entry name" value="EAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription elongation factor Eaf N-terminal" evidence="9">
        <location>
            <begin position="19"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="62"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="119"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="63"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="153"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="204"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF09816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8024584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="319" mass="35492" checksum="F7A70CC2062B413E" modified="2023-09-13" version="1">MDKVQAVCKATEMFGSEVHELKLGRSFYQNKSGAFHTIRYDFKPASIDHSKSASLEVKEGSEVNISFPNDESKGGPSSTNFKGPLKPCQKDCVLIYDHRSGEFTLEKLTSNINVKIQRLEGSSRVKPPSKPTSGKPISHPNKKSGNARMKSPQKREMEGSRIEKEAPSRDTHPQRSLPPSTQRPPTPEVFTRTLSEESSPEAPTVTQNATHKLSQDSSEDEQLSGSASSSEEDEDESSSNNSSSEEEDSVQASNPKKAKYEAPPAANKRRRESAQILNQLREYKLQTILITVMITVLEVWRTDLKTRLAQRLIFVVSCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YQY9</accession>
    <name>A0A9Q0YQY9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_31814</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YQY9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8026859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="102" mass="11525" checksum="FF90B046F9A80D9A" modified="2023-09-13" version="1">MWMCYGRKMNAFIFGGGQRSFGVTGGQTLKALLIRYRMIPYSKEMNPIVCGGVQRSSGVTRGQEAKFPKNSQEFPRIPKNSQDRHLGSPEVKTNFSRIPKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YT29</accession>
    <name>A0A9Q0YT29_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">E3 UFM1-protein ligase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_31242</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UFL1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YT29"/>
    <dbReference type="OrthoDB" id="10258297at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061666">
        <property type="term" value="F:UFM1 ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990592">
        <property type="term" value="P:protein K69-linked ufmylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032434">
        <property type="term" value="P:regulation of proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034976">
        <property type="term" value="P:response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018611">
        <property type="entry name" value="Ufl1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056761">
        <property type="entry name" value="Ufl1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056580">
        <property type="entry name" value="Ufl1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056579">
        <property type="entry name" value="Ufl1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31057">
        <property type="entry name" value="E3 UFM1-PROTEIN LIGASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31057:SF0">
        <property type="entry name" value="E3 UFM1-PROTEIN LIGASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09743">
        <property type="entry name" value="E3_UFM1_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23659">
        <property type="entry name" value="UFL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25041">
        <property type="entry name" value="UFL1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25870">
        <property type="entry name" value="WHD_UFL1_5th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0436">Ligase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="E3 UFM1-protein ligase 1-like N-terminal" evidence="6">
        <location>
            <begin position="8"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="E3 UFM1-protein ligase 1-like" evidence="7">
        <location>
            <begin position="532"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="domain" description="E3 UFM1-protein ligase-like C-terminal" evidence="8">
        <location>
            <begin position="659"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="409"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="741"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="457"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF09743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF25041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8026429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="784" mass="88145" checksum="4067C8362E570726" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-10-08" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YTV3</accession>
    <name>A0A9Q0YTV3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">G-protein coupled receptor GRL101</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HOLleu_32124</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Nanhai2018</strain>
            <tissue evidence="17">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="635273at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008528">
        <property type="term" value="F:G protein-coupled peptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016500">
        <property type="term" value="F:protein-hormone receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009755">
        <property type="term" value="P:hormone-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002131">
        <property type="entry name" value="Gphrmn_rcpt_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24372:SF77">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24372">
        <property type="entry name" value="GLYCOPROTEIN HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00373">
        <property type="entry name" value="GLYCHORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="721"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="931"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="970"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1014"/>
            <end position="1035"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1056"/>
            <end position="1075"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1104"/>
            <end position="1132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1153"/>
            <end position="1177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1189"/>
            <end position="1212"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="14">
        <location>
            <begin position="25"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="14">
        <location>
            <begin position="319"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="14">
        <location>
            <begin position="436"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="15">
        <location>
            <begin position="581"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="16">
        <location>
            <begin position="943"/>
            <end position="1209"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="500"/>
            <end position="517"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ8027087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="19">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1383" mass="158842" checksum="A61FC5121456D0F8" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BD91</accession>
    <name>A0A9Q1BD91_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_40594</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BD91"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007237">
        <property type="entry name" value="CD20-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04103">
        <property type="entry name" value="CD20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="52"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="80"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="136"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="175"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="197"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8020884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="213" mass="23160" checksum="D262341824907D98" modified="2023-09-13" version="1">MRDSTTHHPVMRSLFVTGLFQIVGGMTLFFSGLLLVIMRFDEPYRNRFENPVCGVWTGGFVVIVGIIGVLASGRPGAMRIVYLVTSILTVPSVLTTTVISAVGAADNVHYYAKHPGYNTYDSYDSFEQNEMSITKIAFHLLICFVCATELIIALLVAVFSCNAYCPGTTIKQEHNNAPPPPVTYEPAPSYAYPPPTVANNGESGTSVQAEDKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BFD3</accession>
    <name>A0A9Q1BFD3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_35916</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BFD3"/>
    <dbReference type="OrthoDB" id="775972at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="187"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="256"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="211"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="256"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="268"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="293"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8023463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="410" mass="46398" checksum="1887539831FF2874" modified="2023-09-13" version="1">MTNFRQPTQAFLPAFDATYNGEEDWDCYVERFELFLTANGLDSADCDRNPRVIAMFLHALGAKFYAIVCNLVAPEKPTAKSYTELKSTLRKHLKTTPMVVAERRKFLRRDQVPGESTTDYVVQLKKLSLHCSYGDKLDENLRDRCISGLRDEPTALKLMEKVGEQDNLSFNKAVEFALARESTAESTKAMRSAGETASAAVSASVNTVQRSQRKQPRSRKQCYRCGRNHPPEDCWFKSTECRRCSKIGHIERMCHRRSEEPKAENKKKGQYGKKSYQRKSGKKESQATSKTVNKLDEQVQSENPKEHSDSDSEYDFTMLKLHEEGKVADTGAIYVPIEIEGTVVNMEVDTGSGVSMVPLQFYERHLKHIPLLAGDKSLKSFTGQTVKASGKVYVDVRYGNYEGMDMPASY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BFE4</accession>
    <name>A0A9Q1BFE4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Ribonuclease P protein subunit p25-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_34519</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the histone-like Alba family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BFE4"/>
    <dbReference type="OrthoDB" id="424402at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000172">
        <property type="term" value="C:ribonuclease MRP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.20">
        <property type="entry name" value="Alba-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036882">
        <property type="entry name" value="Alba-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051958">
        <property type="entry name" value="Alba-like_NAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002775">
        <property type="entry name" value="DNA/RNA-bd_Alba-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13516:SF4">
        <property type="entry name" value="FI09323P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13516">
        <property type="entry name" value="RIBONUCLEASE P SUBUNIT P25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01918">
        <property type="entry name" value="Alba"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82704">
        <property type="entry name" value="AlbA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA/RNA-binding protein Alba-like" evidence="5">
        <location>
            <begin position="32"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="130"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="155"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="185"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="198"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="224"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8024575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="239" mass="27318" checksum="A10FE7E71C2159D3" modified="2023-09-13" version="1">MENYTKGETVDVTEEDPFAKLNLQGWTDRIKMNIHAGGKIRNYIGFASKEFKKEERRLITFSGSGDAVSKVISCVEIMKRNHKGGLHQITKIFYTKKEDLWEPKKEDLDRLKVTRHIPAISVLLSKDQLDTNEPGYQAPGSFEEDWGKSTAQANAKRKNYAQKRRSRLHQPRGPASSAHGSGRGDNFRGKNSESEYPKRKHNKYRRKENHSDKNKTGKGVGENGGRKEKDDDVTEKNRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BFS3</accession>
    <name>A0A9Q1BFS3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_33852</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8026106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BFS3"/>
    <dbReference type="OrthoDB" id="6502630at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8026106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="128" mass="14254" checksum="19D8A56F4C31D31B" modified="2023-09-13" version="1">MPHIKRKNPIVFGGGQRSPGVTGGQTLKILLTQYLEVGSLDKFHTLYMRYDKRKNPIFFGGGQRSSGVTGGQTLKTLLTQYLKMCYGKRKNPIIFGAGQRSSGVTGGQTLKTMLTRYLKIGSLDRFHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BHC9</accession>
    <name>A0A9Q1BHC9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">GTP-binding protein ypt5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_31542</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BHC9"/>
    <dbReference type="OrthoDB" id="6056409at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="174"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="184"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8026645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="538" mass="60695" checksum="EB1F1356B397137E" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BHW2</accession>
    <name>A0A9Q1BHW2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">RalBP1-associated Eps domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_31653</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BHW2"/>
    <dbReference type="OrthoDB" id="207120at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00052">
        <property type="entry name" value="EH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000261">
        <property type="entry name" value="EH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11216">
        <property type="entry name" value="EH DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11216:SF174">
        <property type="entry name" value="GH06923P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12763">
        <property type="entry name" value="EH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00027">
        <property type="entry name" value="EH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50031">
        <property type="entry name" value="EH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EH" evidence="2">
        <location>
            <begin position="10"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="EH" evidence="2">
        <location>
            <begin position="308"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="345"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="103"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="418"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="717"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="175"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="190"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="253"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="273"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="291"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="498"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="556"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="578"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="744"/>
            <end position="761"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8026733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="836" mass="92319" checksum="1F8945F1BEA4CED6" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BHY7</accession>
    <name>A0A9Q1BHY7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Glycoprotein endo-alpha-1,2-mannosidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_31824</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 99 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BHY7"/>
    <dbReference type="OrthoDB" id="406152at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004559">
        <property type="term" value="F:alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11574">
        <property type="entry name" value="GH99"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000177">
        <property type="entry name" value="MANEAL isoform 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026071">
        <property type="entry name" value="Glyco_Hydrolase_99"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13572">
        <property type="entry name" value="ENDO-ALPHA-1,2-MANNOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13572:SF4">
        <property type="entry name" value="RE57134P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16317">
        <property type="entry name" value="Glyco_hydro_99"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8026868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="498" mass="57743" checksum="A67E649E10D09581" modified="2023-09-13" version="1">MIPRRFRSLRCIATFIVTLFLLGCISAVVVITRVVDLEGPMSLLEPFERGKSLMERNHGENKQQRENVIKESHDDKMADDEKAERGKKFINDMKGNVQNMTKLKSPRKKKLIKSEKIPKRNVSVTSYLREDVKKDSRIWSDIQPNYNLHAFYYPWYGNPDHNGNYYHWNHEYIPHWNKNVAKKWVTGIHSPPHDVGSNFYPHLGCYSSSDDDIIGEHMKQMRFAGIGVLVLSWYPPGKADGQGIPSDGLVPKIMDIAHEHQLKVAFHSEPYNGRSESTFANDVKYIVDSYGSHPAFYKYQGSKKAKEALPVFYLYDSYLLNATFWANIFRHGSPKSLRGTRYDGIFLALFVESKHRSEVLRGGFDGFYTYFASNRFTYGSTWTSWKILRQFASRSNLLFVPSVGPGYIDTRVRPWNGINTKWRDHGAYYKGSFEAAMSLNPPIVSITSFNEWHEGTQIEKAIPYRYGNFSYLDYTPNQPDYYLRLTRQLSEDLKHPNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BI43</accession>
    <name>A0A9Q1BI43_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_34105</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8026299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BI43"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8026299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="78" mass="9279" checksum="84B0422218E4E66E" modified="2023-09-13" version="1">MDACPSKPKRRVILPFKIASCWLKSRDKSLYRGYARDVSAFCWMELIMRFQYFQPVFITCFKLLEMCGIFWCSTSCND</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BIN3</accession>
    <name>A0A9Q1BIN3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cullin-5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_32411</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7 8">Belongs to the cullin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BIN3"/>
    <dbReference type="OrthoDB" id="27073at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031461">
        <property type="term" value="C:cullin-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000142">
        <property type="entry name" value="Cullin 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000009">
        <property type="entry name" value="Cullin 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.130:FF:000004">
        <property type="entry name" value="Cullin 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1310.10">
        <property type="entry name" value="Cullin Repeats"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.130">
        <property type="entry name" value="Cullin, Chain C, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045093">
        <property type="entry name" value="Cullin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059120">
        <property type="entry name" value="Cullin-like_AB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016157">
        <property type="entry name" value="Cullin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016158">
        <property type="entry name" value="Cullin_homology"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036317">
        <property type="entry name" value="Cullin_homology_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001373">
        <property type="entry name" value="Cullin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019559">
        <property type="entry name" value="Cullin_neddylation_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016159">
        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11932">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00888">
        <property type="entry name" value="Cullin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26557">
        <property type="entry name" value="Cullin_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10557">
        <property type="entry name" value="Cullin_Nedd8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00182">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00884">
        <property type="entry name" value="Cullin_Nedd8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75632">
        <property type="entry name" value="Cullin homology domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01256">
        <property type="entry name" value="CULLIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50069">
        <property type="entry name" value="CULLIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Cullin family profile" evidence="9">
        <location>
            <begin position="74"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8027302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="450" mass="52889" checksum="20CC5B3D0391F881" modified="2023-09-13" version="1">MNLPKDSEKYVERLLELFHRFSTLVKEAFNDDPRFLTARDKAYEHVVNDTTVFRLELASNKSKSVVSKTQPESKCPELLANYCDMLLRRTPLSKKLTSEEIETKLKNVLLVLKYVQNKDVFMRYHKVHLTRRLILDTSADSEKEENMVEWLREVGMPADYVNKLARMFQDIKVSEDLNASFRELHRNNQSSISDNINIKILNAGAWSRSSERVPVSLPLELEDFIPEVEEFYKNKHSGRKLQWHHLMSNGIISFKNDIGVFDLEVTTFQMAVLFAWNQRPKDKISFENLRLATELPENELRKTLWSLIAFAKLKRQVLLCRPECKSPKEFTDSTLFSINQQFAMIKNGKPQKRGKVNMIGRLQLSTDRSTDEDKEGIVQLRILRTQEAILKIMKMRKKISNAQLQTELVEILKNMFLPQKKMIKEQIEWLIEHKYMKRDENDINLFIYLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BIQ5</accession>
    <name>A0A9Q1BIQ5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_32459</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BIQ5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040223550" evidence="1">
        <location>
            <begin position="25"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8027340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="103" mass="11435" checksum="1636FF5C56D395CB" modified="2023-09-13" version="1">MMDSKRERLVVLFTFMAFCELTAAMGLCKFGRAFPSQGKLNGTTFDNVAKVIDHLTSLTSTKNQEDFSTSTKQSLKMQNATMKDESTIKSGTVYKLSPDLLIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BJR5</accession>
    <name>A0A9Q1BJR5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Vacuolar ATPase assembly integral membrane protein vma21</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_29917</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8027846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BJR5"/>
    <dbReference type="OrthoDB" id="160405at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070072">
        <property type="term" value="P:vacuolar proton-transporting V-type ATPase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019013">
        <property type="entry name" value="Vma21"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31792">
        <property type="entry name" value="VACUOLAR ATPASE ASSEMBLY INTEGRAL MEMBRANE PROTEIN VMA21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31792:SF3">
        <property type="entry name" value="VACUOLAR ATPASE ASSEMBLY INTEGRAL MEMBRANE PROTEIN VMA21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09446">
        <property type="entry name" value="VMA21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="2" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="30"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="71"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8027846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="104" mass="11353" checksum="7C9E46CFD1248754" modified="2023-09-13" version="1">MQSASIKTTEHKNIKMPADLGYSEAREKSVALTAIFYTLLVIFVPMVSYSFSKSIIFDGLLGLDNSTSTTAAAVAAVVGVHVVLVFFIIAAYREDRRPSPPKRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BKQ4</accession>
    <name>A0A9Q1BKQ4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="17">ATP-dependent DNA helicase DDX11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HOLleu_30585</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Nanhai2018</strain>
            <tissue evidence="17">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DEAD box helicase family. DEAH subfamily. DDX11/CHL1 sub-subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BKQ4"/>
    <dbReference type="OrthoDB" id="267079at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016818">
        <property type="term" value="F:hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034085">
        <property type="term" value="P:establishment of sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18788">
        <property type="entry name" value="SF2_C_XPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001050">
        <property type="entry name" value="ATP-dependent DNA helicase DDX11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002532">
        <property type="entry name" value="DEAD/H-box helicase 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006555">
        <property type="entry name" value="ATP-dep_Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045028">
        <property type="entry name" value="DinG/Rad3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014013">
        <property type="entry name" value="Helic_SF1/SF2_ATP-bd_DinG/Rad3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006554">
        <property type="entry name" value="Helicase-like_DEXD_c2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010614">
        <property type="entry name" value="RAD3-like_helicase_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013020">
        <property type="entry name" value="Rad3/Chl1-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00604">
        <property type="entry name" value="rad3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472:SF41">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE DDX11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472">
        <property type="entry name" value="DNA REPAIR DEAD HELICASE RAD3/XP-D SUBFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06733">
        <property type="entry name" value="DEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13307">
        <property type="entry name" value="Helicase_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00488">
        <property type="entry name" value="DEXDc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00491">
        <property type="entry name" value="HELICc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51193">
        <property type="entry name" value="HELICASE_ATP_BIND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 17" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="11" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="13" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="16">
        <location>
            <begin position="14"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="82"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="145"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="274"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="87"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="161"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="175"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="196"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="278"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ8028374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="901" mass="101744" checksum="54BD438FD2D6BEB5" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BLI3</accession>
    <name>A0A9Q1BLI3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_31096</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BLI3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040484906" description="Secreted protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8028764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="116" mass="13001" checksum="E5A630581285A8FD" modified="2023-09-13" version="1">MCVCACVCVCVCVTLASVRCNSTRESWTKPKLGIWICHSKKVCPIVFGGGQKSCGVTRGQRPKTLFARYLKMTYGEKMNPLIFGGGQRSFGVTESQILKTLLMQYLKEKILDRFHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BNE8</accession>
    <name>A0A9Q1BNE8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Ammonium transporter 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_29373</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BNE8"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008519">
        <property type="term" value="F:ammonium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097272">
        <property type="term" value="P:ammonium homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3430.10">
        <property type="entry name" value="Ammonium transporter AmtB like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029020">
        <property type="entry name" value="Ammonium/urea_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024041">
        <property type="entry name" value="NH4_transpt_AmtB-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11730">
        <property type="entry name" value="AMMONIUM TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11730:SF58">
        <property type="entry name" value="AMMONIUM TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00909">
        <property type="entry name" value="Ammonium_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111352">
        <property type="entry name" value="Ammonium transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="26"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="domain" description="Ammonium transporter AmtB-like" evidence="6">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8029861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="66" mass="7331" checksum="49151376AC7E0DE6" modified="2023-09-13" version="1">MLGPRTGRFDSSEAGVVSEMGSPTNAVLGLFMLWWGWLGFNCGSTYGIANKKWVLATRYVNLRVLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BPA3</accession>
    <name>A0A9Q1BPA3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">AMMECR1-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_26699</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BPA3"/>
    <dbReference type="OrthoDB" id="24630at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.700.20:FF:000001">
        <property type="entry name" value="AMME syndrome candidate gene 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.700.20">
        <property type="entry name" value="Hypothetical protein ph0010, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023473">
        <property type="entry name" value="AMMECR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036071">
        <property type="entry name" value="AMMECR1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002733">
        <property type="entry name" value="AMMECR1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027485">
        <property type="entry name" value="AMMECR1_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00296">
        <property type="entry name" value="TIGR00296 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13016:SF0">
        <property type="entry name" value="AMME SYNDROME CANDIDATE GENE 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13016">
        <property type="entry name" value="AMMECR1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01871">
        <property type="entry name" value="AMMECR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143447">
        <property type="entry name" value="AMMECR1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51112">
        <property type="entry name" value="AMMECR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040443025" evidence="2">
        <location>
            <begin position="32"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="AMMECR1" evidence="3">
        <location>
            <begin position="92"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8030322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="292" mass="33663" checksum="6B6218D87F140892" modified="2023-09-13" version="1">MELPKHCPRWQSTPVAHACLSLFVIVSLKMATGCCGVKRQKVSVPTEKDETENKDRPSSSQNSNNRGHSNHVGNGKVVSIPRNRINGYNHVPDKTQRVVCPEMCYFCFDVLTRRLHSVEMPKTPLKFTDAPFPLFVTWKIDNERKLRGCIGTFTAMNLHDGLREYAVSSAFKDSRFPPINREELSRLHCSVSLLRHFEQAKDHQDWEVGKHGIRIEFYNEKGTRKTATYLPEVASEQRWDRIQTIDSLLRKGGYKSSITNEFRKTVSVTRYQSEKLSISYAEYISNRKNGYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BPD2</accession>
    <name>A0A9Q1BPD2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_26540</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BPD2"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="103"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8030201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="150" mass="16788" checksum="DF7C39A3074E7602" modified="2023-09-13" version="1">MYNAVEDAVWLTSERKHTRWNTDAGFVNKGSLPYPTSRHTCWRPTTREDFAVIGANIHQVERQMGKSLGPHGAMPPRWKGEDKETVGETPKAERHAPSLDSYPFLDTLLTPTPSLEELGDPENANALIQESPASPRLPSPRPPTPPRNFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BPF8</accession>
    <name>A0A9Q1BPF8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_26518</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BPF8"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8030184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="78" mass="8576" checksum="24029655B51DABA5" modified="2023-09-13" version="1">MLREEDLTLEKAINTCRAAESSKKSGKTLTGSEQACYSVSQKKWGNSAKPSRSKYRPKGTKPNNESTKPKSLGQTQRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BPU5</accession>
    <name>A0A9Q1BPU5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_27036</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BPU5"/>
    <dbReference type="OrthoDB" id="5986462at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8030580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="169" mass="19298" checksum="5E18FDFDFFBED4A4" modified="2023-09-13" version="1">MAFKKVEESIAVFSRTPPSVEELHHHMPTIERLLSSCMRRQVHVNVWMMPAKKLFTRKGRVIELIPPTSEALFHHAKRAVYQAAYVWGQALLRDPELPDPSDWGWTKRTSGLWKPLWTTLPEACTTCQELIKFDCNVDKGCHGRCKCVKAGVACTTYCKCYGDCKHSCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BS23</accession>
    <name>A0A9Q1BS23_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_24856</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BS23"/>
    <dbReference type="OrthoDB" id="10021899at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8031610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="124" mass="13562" checksum="D2D1E02DDE5FBCB3" modified="2023-09-13" version="1">MVSIEYSAHNYNQFFVLQFSVRGAGRIVKGARVGIGAAVDPDLGDAEWDVQYEEAGDVQRTITGGSFTCNNQRTCRAINNYGPYRVTNLAVKNINRDHRTFDLVFSAPGADLMDGTGKGTKNVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BSD4</accession>
    <name>A0A9Q1BSD4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Sulfotransferase 1 family member D1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_25276</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sulfotransferase 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BSD4"/>
    <dbReference type="OrthoDB" id="205623at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11783">
        <property type="entry name" value="SULFOTRANSFERASE SULT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sulfotransferase" evidence="3">
        <location>
            <begin position="38"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8031912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="302" mass="35313" checksum="369B30321F88A501" modified="2023-09-13" version="1">MSIQEDPVLRYYKGPTPFFPLIKDAAIDAVRSFTYREDDILVATYPKCGTHFVNEVIQLMIHDGDPTKLDHRHRRICLEIADTTDFSKVGEVKSSVELVDTDPSPRVLMTHLCSSYLSNETWQKRIPIVYVFRDPRDAVLSYYEFMKHFQLREGEPFLQGKSFEQYLSEYIKGKVPFGGWCEHALSYEERFKNGENILFIYYEDMKKDLSTVIKKIAKHISRPVSDDVIGKTLANSTVDAMRQNYTEANKMKRKVNIDLSKFIKTGVSGNWKKDHSEQTWKHLNEIFREMVKDTQFAKTYFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BSQ8</accession>
    <name>A0A9Q1BSQ8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Ras-related protein R-Ras2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_25352</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Ras family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BSQ8"/>
    <dbReference type="OrthoDB" id="5976022at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000080">
        <property type="entry name" value="Ras-like GTPase Ras1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020849">
        <property type="entry name" value="Small_GTPase_Ras-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24070">
        <property type="entry name" value="RAS, DI-RAS, AND RHEB FAMILY MEMBERS OF SMALL GTPASE SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0636">Prenylation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8031970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="200" mass="22427" checksum="FF322AB38795383C" modified="2023-09-13" version="1">MAGGVGAVNSIQTHKIVVVGGGGVGKSALTIQFIQSYFVADYDPTIEDSYMKQCVIDNTPCKLDILDTAGQDEFSAMREQYMRSGEGFLLVFSLTNRTSFDEIYKYHRQILRVKDRDEFPMILVGNKCDLESSRQVTTEEGNQLAQTLKIAYLEASAKNRTNVDQSFHKLVRLIRSFQEEENPPLAPDDKKEPSSCCVLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BT10</accession>
    <name>A0A9Q1BT10_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Zinc finger and SCAN domain-containing protein 5B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HOLleu_25531</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Nanhai2018</strain>
            <tissue evidence="15">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the histone deacetylase HD2 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="Nucleoplasmin core domain"/>
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    </dbReference>
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        <property type="entry name" value="Zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
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        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515:SF49">
        <property type="entry name" value="GASTRULA ZINC FINGER PROTEIN XLCGF49.1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17800">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="362"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="390"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="418"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="98"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="200"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="461"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="115"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="228"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022833"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
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            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
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        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
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            <dbReference type="EMBL" id="KAJ8032109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="483" mass="54848" checksum="658CBA205D4F850E" modified="2023-09-13" version="1">MIWGLAIEPGQMYSCTLDEEVHLSLAALESRPESDEKASKYSQVVLKTKDTQQLLCTLVHGTIFQQSLDLKLMPRENVSFSVQGSGVVYITGYSYSSPVEEGEEESGEFNTSQDLQEEAEVELQEDEERYEDEEISMDIHIKGEPVNTHQDEMYEKGEHSIPDVGRKIEQNASVSENRLMEVPAPDEATQLETTHTVAAIDNLEEPNLPQTSDSGWLNEPSYPPQVEVSERTAERMTSHDPSPAANERSFTEAPGTSRWINSRMTSYPQQGEQVYSQPARNPGGHRTMAIPRGAMSRGDQAVYSKPVPRTVKHRWAMPRTVSQEEDILQGNQQMQCRFCRSVFPNFEMKKCHEKTHTEARAYRCLYCVKSFKSHGHRQQHERIHTGVKPFKCRICTKGFAQSGDRNRHERIHSANKPYQCRHCQKFFRDYTYAVRHERTQHHSITMVASGEEGENVGLMGDSSSGSDMTMLRDHNVGEDGEGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BV88</accession>
    <name>A0A9Q1BV88_HOLLE</name>
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        <submittedName>
            <fullName evidence="4">Ficolin-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_23364</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Eleutherozoa</taxon>
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            <taxon>Holothuroidea</taxon>
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            <taxon>Aspidochirotida</taxon>
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            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="KAJ8033201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Ser_inhib-like_sf"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="FIBRINOGEN/TENASCIN/ANGIOPOEITIN"/>
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    </dbReference>
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        <property type="entry name" value="PROTEIN SCABROUS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
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        <property type="match status" value="2"/>
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        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040492676" evidence="2">
        <location>
            <begin position="22"/>
            <end position="536"/>
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    </feature>
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            <begin position="47"/>
            <end position="186"/>
        </location>
    </feature>
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        <location>
            <begin position="307"/>
            <end position="536"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAJ8033201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="536" mass="61030" checksum="3B8889927B6B2FE5" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BVT0</accession>
    <name>A0A9Q1BVT0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Class E basic helix-loop-helix protein 22</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_24012</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BVT0"/>
    <dbReference type="OrthoDB" id="10011855at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070888">
        <property type="term" value="F:E-box binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061564">
        <property type="term" value="P:axon development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007423">
        <property type="term" value="P:sensory organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050359">
        <property type="entry name" value="bHLH_transcription_factors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19290">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX PROTEIN NEUROGENIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19290:SF104">
        <property type="entry name" value="GH17679P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BHLH" evidence="2">
        <location>
            <begin position="68"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8033685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="117" mass="14034" checksum="30FD6A37EDE0B468" modified="2023-09-13" version="1">MENTTDKRQCQDIMFNRIDGTAPNLGEAILKPSTPCAGGTSAIKVRRSSYKSSHSKRVEEHQSQRQVRLNTNARERKRMHDLNDALDDLRAEERERERERERERERERERERERERE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BY96</accession>
    <name>A0A9Q1BY96_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phospholipid transfer protein C2CD2L</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_21737</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BY96"/>
    <dbReference type="OrthoDB" id="9976063at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039934">
        <property type="entry name" value="C2CD2/C2CD2L"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040885">
        <property type="entry name" value="SMP_C2CD2L"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21119">
        <property type="entry name" value="C2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21119:SF5">
        <property type="entry name" value="C2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18696">
        <property type="entry name" value="SMP_C2CD2L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="2">
        <location>
            <begin position="156"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="433"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="641"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="435"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="477"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="490"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="514"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="556"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="593"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="646"/>
            <end position="662"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8034764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="662" mass="73817" checksum="7F8A885FE48C27C5" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BYA6</accession>
    <name>A0A9Q1BYA6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Synaptogenesis protein syg-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_21731</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BYA6"/>
    <dbReference type="OrthoDB" id="6413693at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00099">
        <property type="entry name" value="IgV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013162">
        <property type="entry name" value="CD80_C2-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45889">
        <property type="entry name" value="IG-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45889:SF8">
        <property type="entry name" value="IG-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08205">
        <property type="entry name" value="C2-set_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="594"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="72"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="179"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="287"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="398"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="493"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="657"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="739"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="664"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="765"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="795"/>
            <end position="806"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8034759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="890" mass="98121" checksum="5B1FAE096480E3A8" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C036</accession>
    <name>A0A9Q1C036_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 14">Signal peptidase complex catalytic subunit SEC11</fullName>
            <ecNumber evidence="4 14">3.4.21.89</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HOLleu_20012</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Nanhai2018</strain>
            <tissue evidence="16">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Catalytic component of the signal peptidase complex (SPC) which catalyzes the cleavage of N-terminal signal sequences from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum. Specifically cleaves N-terminal signal peptides that contain a hydrophobic alpha-helix (h-region) shorter than 18-20 amino acids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.</text>
            <dbReference type="EC" id="3.4.21.89"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="14">Component of the signal peptidase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the peptidase S26B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.89" evidence="4 14"/>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C036"/>
    <dbReference type="OrthoDB" id="10257561at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005787">
        <property type="term" value="C:signal peptidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009003">
        <property type="term" value="F:signal peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06530">
        <property type="entry name" value="S26_SPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.109.10:FF:000003">
        <property type="entry name" value="Signal peptidase complex catalytic subunit SEC11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036286">
        <property type="entry name" value="LexA/Signal_pep-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019758">
        <property type="entry name" value="Pept_S26A_signal_pept_1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019756">
        <property type="entry name" value="Pept_S26A_signal_pept_1_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015927">
        <property type="entry name" value="Peptidase_S24_S26A/B/C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019533">
        <property type="entry name" value="Peptidase_S26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001733">
        <property type="entry name" value="Peptidase_S26B"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02228">
        <property type="entry name" value="sigpep_I_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10806">
        <property type="entry name" value="SIGNAL PEPTIDASE COMPLEX CATALYTIC SUBUNIT SEC11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10806:SF6">
        <property type="entry name" value="SIGNAL PEPTIDASE COMPLEX CATALYTIC SUBUNIT SEC11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00717">
        <property type="entry name" value="Peptidase_S24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00728">
        <property type="entry name" value="SIGNALPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51306">
        <property type="entry name" value="LexA/Signal peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00501">
        <property type="entry name" value="SPASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00761">
        <property type="entry name" value="SPASE_I_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 14" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 14" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="7 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="18"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S24/S26A/S26B/S26C" evidence="15">
        <location>
            <begin position="36"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU362047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ8036131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="179" mass="20506" checksum="EAF919483192FC3D" modified="2023-09-13" version="1">MASFDFLDEVRRMNKRQLFYQVLNFGMIVSSALMIWKGLMVVTASESPIVVVLSGSMEPAFFRGDLLFLTNHQDDPIRVGEIVVFKIEGRDIPIVHRVLRLHEKENGDVKFLTKGDNNMVDDRGLYAPGQLWLERKDVVGRARGYVPYIGMVTILMNDYPKFKYLVLAGLGIFVLIHRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C0W1</accession>
    <name>A0A9Q1C0W1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Collagen triple helix repeat-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_20132</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C0W1"/>
    <dbReference type="OrthoDB" id="5985978at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005581">
        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057873">
        <property type="entry name" value="CTHRC1_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25815">
        <property type="entry name" value="CTHRC1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0176">Collagen</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040514130" evidence="1">
        <location>
            <begin position="23"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="CTHRC1 C-terminal" evidence="2">
        <location>
            <begin position="44"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8036218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="92" mass="9547" checksum="8DAD06D7AE5FACA6" modified="2023-09-13" version="1">MEIFIWFLVIVLPVFIISSGKASQQCVGPRGHSGDQGDDGAVGSGVCDGLSAGSIDVTINAGPCSGYGTSDLKTGWNAVSRILIEEWPSSPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C4X9</accession>
    <name>A0A9Q1C4X9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pre-mRNA-splicing factor 38</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_15962</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Required for pre-mRNA splicing.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the PRP38 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C4X9"/>
    <dbReference type="OrthoDB" id="3881at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005037">
        <property type="entry name" value="PRP38"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23142">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR 38A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03371">
        <property type="entry name" value="PRP38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5 7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="75"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="117"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="249"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="249"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="291"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="311"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="332"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="357"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="368"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="417"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8038515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="451" mass="53159" checksum="AFB89122C8706257" modified="2023-09-13" version="1">MPPKNNTLPTWGNEASMNLNSLILTNIQSSPYFKNELIKLKTYHEVIDEIYYKVDHLEPWERGSRQTSGQIGMCGGVRGVGAGGIVSSAYCLLFKLYTLKLTRKQVMGVLNHTDSPYIRGLGFMYIRSVFQFVVVAMYVILIDNARFYHGWNLNFSHLLCPMFLIDIVSLPRYSQPPQDLFDWFEPYFDDEEEIDLKAGSGAPTTIGQMVRSMLVKQEWFSTLFPRIPVPVAKDIERRFAERKKINAGKGEDVKEEAAVAKEGSRKASPEADDPGNGRQSPDHSRKKSHSRSREEEKGSGRNHERSGSKGRDKRSRSRDKRNRSRDRERRRSRERRSRSRDRRQRSKERKRSRSPGQRRDDKDDDRRRRPSPPRDRRRSRSRERDRRRRSRSRERRKSRSRERKSPVRRATPVDFADELRRERERQKRQGKRPSRSKSRERSSRRSRSHSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C5B6</accession>
    <name>A0A9Q1C5B6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_15881</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C5B6"/>
    <dbReference type="OrthoDB" id="1933708at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon gypsy-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF13">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCAN box" evidence="2">
        <location>
            <begin position="242"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase zinc-binding" evidence="3">
        <location>
            <begin position="794"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="337"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="600"/>
            <end position="688"/>
        </location>
    </feature>
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        <location>
            <begin position="700"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="351"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="375"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="600"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="644"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="669"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="716"/>
            <end position="740"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF17921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8038450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="922" mass="104829" checksum="8888FA2CDB774E5C" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C6S2</accession>
    <name>A0A9Q1C6S2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Kinesin-associated protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_17104</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C6S2"/>
    <dbReference type="OrthoDB" id="10265679at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035869">
        <property type="term" value="C:ciliary transition zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016939">
        <property type="term" value="C:kinesin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019894">
        <property type="term" value="F:kinesin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044782">
        <property type="term" value="P:cilium organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008658">
        <property type="entry name" value="KAP3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15605:SF2">
        <property type="entry name" value="KINESIN-ASSOCIATED PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15605">
        <property type="entry name" value="KINESIN-ASSOCIATED PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05804">
        <property type="entry name" value="KAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM01297">
        <property type="entry name" value="KAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ARM" evidence="1">
        <location>
            <begin position="588"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="745"/>
            <end position="764"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8039397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="764" mass="87529" checksum="4D7194303167C119" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C6X0</accession>
    <name>A0A9Q1C6X0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Disabled-like 2-interacting protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_17285</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="5572587at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="C2_SynGAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RasGAP_DAB2IP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.506.10">
        <property type="entry name" value="GTPase Activation - p120gap, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="C2_dom"/>
    </dbReference>
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        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021887">
        <property type="entry name" value="DAB2P_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039360">
        <property type="entry name" value="Ras_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023152">
        <property type="entry name" value="RasGAP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001936">
        <property type="entry name" value="RasGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057606">
        <property type="entry name" value="SynGAP1-like_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10194:SF60">
        <property type="entry name" value="RAS GTPASE-ACTIVATING PROTEIN RASKOL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10194">
        <property type="entry name" value="RAS GTPASE-ACTIVATING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12004">
        <property type="entry name" value="DAB2P_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25321">
        <property type="entry name" value="PH_RASGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00616">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00323">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00509">
        <property type="entry name" value="RAS_GTPASE_ACTIV_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50018">
        <property type="entry name" value="RAS_GTPASE_ACTIV_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="4">
        <location>
            <begin position="178"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GAP" evidence="5">
        <location>
            <begin position="356"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="43"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="718"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="920"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1087"/>
            <end position="1132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1225"/>
            <end position="1261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="718"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="757"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="768"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="848"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="920"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1093"/>
            <end position="1108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1225"/>
            <end position="1244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1252"/>
            <end position="1261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8039535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1353" mass="151555" checksum="A149A3ACD7D96926" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C804</accession>
    <name>A0A9Q1C804_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Medium-chain specific acyl-CoA dehydrogenase, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_17338</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C804"/>
    <dbReference type="OrthoDB" id="434771at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070991">
        <property type="term" value="F:medium-chain fatty acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051793">
        <property type="term" value="P:medium-chain fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.540.10:FF:000010">
        <property type="entry name" value="Medium-chain specific acyl-CoA dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000011">
        <property type="entry name" value="Medium-chain specific acyl-CoA dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.110.10:FF:000006">
        <property type="entry name" value="very long-chain specific acyl-CoA dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050741">
        <property type="entry name" value="Acyl-CoA_dehydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48083:SF2">
        <property type="entry name" value="MEDIUM-CHAIN SPECIFIC ACYL-COA DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48083">
        <property type="entry name" value="MEDIUM-CHAIN SPECIFIC ACYL-COA DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00072">
        <property type="entry name" value="ACYL_COA_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00073">
        <property type="entry name" value="ACYL_COA_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0274">FAD</keyword>
    <keyword evidence="4 7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="10">
        <location>
            <begin position="73"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="9">
        <location>
            <begin position="187"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="8">
        <location>
            <begin position="299"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8039576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="456" mass="50227" checksum="B5189B93DF3C930A" modified="2023-09-13" version="1">MHAAPTADCRSFTIVFTVRSKIKSKMSQKFQLIRRAFQALNASQLRWKSSAASPSAQGSAAKVPEGLSFALSNEQQEYKDLAAKFVEDKVIPKAAEYDKSGEYPMDVITECWNLGLMNTHIPQEYGGLGLGILDACIVTEELAYGCTGIQTAIEANSLGQMPLILAGNDEQKKKYLGRMMEEPLMCAYGVTEPIAGSDVSGIATRAVKKGDEYILNGSKMWITNGGKANWYFVLARTDENPLVCPTKSKEFTGFIVEREWSGVEPGRKEWNMGQRASDTRGITFTDVRIPKENVLAVQGAGFKIAMGAFDKTRPPVAAGAVGLARRAMDEATKYSLERTAFGKKISEFQAVQFILAEMAINVEAARLLTQRSAWEIDLGKRNTYFASIAKAFAADIANKCATDAVQVFGGNGFNSDYPVEKLMRDAKIYQIYEGTAQIQRVIISRELLSKARERWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C821</accession>
    <name>A0A9Q1C821_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cell division cycle protein 20 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">p55CDC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HOLleu_14040</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Nanhai2018</strain>
            <tissue evidence="16">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Substrate-specific adapter of the anaphase promoting complex/cyclosome (APC/C) complex that confers substrate specificity by binding to substrates and targeting them to the APC/C complex for ubiquitination and degradation. Recognizes and binds the destruction box (D box) on protein substrates. Involved in the metaphase/anaphase transition of cell cycle. Is regulated by MAD2L1: in metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 induces presynaptic differentiation. The CDC20-APC/C complex promotes proper dilation formation and radial migration by degrading CCDC41.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of a complex with CDC20, CDC27, SPATC1 and TUBG1. Interacts with NEUROD2. Interacts with dimeric MAD2L1 in its closed conformation form. Interacts with BUB1B. The phosphorylated form interacts with APC/C. Interacts with NINL. May interact with MAD2L2. Interacts with CDK5RAP2. Interacts with SIRT2. Interacts with isoform 1 of NEK2. Interacts with HSF1 (via phosphorylated form); this interaction occurs in mitosis in a MAD2L1-dependent manner and prevents PLK1-stimulated degradation of HSF1 by blocking the recruitment of the SCF(BTRC) ubiquitin ligase complex. Interacts (via the N-terminal substrate-binding domain) with FBXO5. Interacts with CCNF. Interacts with USP22.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat CDC20/Fizzy family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8039890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C821"/>
    <dbReference type="OrthoDB" id="10263272at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010997">
        <property type="term" value="F:anaphase-promoting complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990757">
        <property type="term" value="F:ubiquitin ligase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905786">
        <property type="term" value="P:positive regulation of anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000224">
        <property type="entry name" value="cell division cycle protein 20 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033010">
        <property type="entry name" value="Cdc20/Fizzy"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056150">
        <property type="entry name" value="WD40_CDC20-Fz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19918">
        <property type="entry name" value="CELL DIVISION CYCLE 20 CDC20 FIZZY -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19918:SF8">
        <property type="entry name" value="FI02843P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24807">
        <property type="entry name" value="WD40_CDC20-Fz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4 16" id="KW-0132">Cell division</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="3 13" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="CDC20/Fizzy WD40" evidence="15">
        <location>
            <begin position="192"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="13">
        <location>
            <begin position="238"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="13">
        <location>
            <begin position="321"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="13">
        <location>
            <begin position="454"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="62"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="494"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="73"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00062231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00070718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00079207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00093365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF24807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ8039890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="513" mass="56401" checksum="0635B7E54F3FBB56" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C8F5</accession>
    <name>A0A9Q1C8F5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Sodium/myo-inositol cotransporter 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_13996</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8039861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C8F5"/>
    <dbReference type="OrthoDB" id="6132759at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005412">
        <property type="term" value="F:D-glucose:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="entry name" value="Sodium/glucose cotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018212">
        <property type="entry name" value="Na/solute_symporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00813">
        <property type="entry name" value="sss"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11819:SF196">
        <property type="entry name" value="SODIUM_GLUCOSE COTRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11819">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00456">
        <property type="entry name" value="NA_SOLUT_SYMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00457">
        <property type="entry name" value="NA_SOLUT_SYMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040361644" evidence="8">
        <location>
            <begin position="19"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="34"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="77"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="111"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="145"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="207"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="246"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="313"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="355"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="392"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="418"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="459"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="582"/>
            <end position="602"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8039861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="603" mass="65660" checksum="FCBB663DBC21E109" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C8H4</accession>
    <name>A0A9Q1C8H4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Sulfotransferase family cytosolic 1B member 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_14266</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sulfotransferase 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C8H4"/>
    <dbReference type="OrthoDB" id="205623at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11783">
        <property type="entry name" value="SULFOTRANSFERASE SULT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sulfotransferase" evidence="3">
        <location>
            <begin position="47"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8040074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="282" mass="32683" checksum="E828F5B292233906" modified="2023-09-13" version="1">MSGKAVALDKGSMAIIANTFEYDGVWYPPFVRPHILDRIRTFEVREDDLILSTFSKCGTNWMREIILLVYQDGHVDKVNRDVDPSLCGMLELGHPPDFNKSVADYVAALPSPRFYYTHLPAQHLPAQVWTKKPKMVYVTRNPKDALTSLYRFLNANPANTCEVPWRMLFEIFISKKIPFGNWFDQALAYWEHRDDGHVCFVTYEEMKQTYMQDIRSVIKRLATFLGVTVSSDGLQRIVEHSSLEGMKMTYAKIEEEVENGKMYTRSQGQIPYLQQGKERIKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C8J7</accession>
    <name>A0A9Q1C8J7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Sia-alpha-2,3-Gal-beta-1,4-GlcNAc-R:alpha 2,8-sialyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_14208</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 29 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C8J7"/>
    <dbReference type="OrthoDB" id="10264956at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003828">
        <property type="term" value="F:alpha-N-acetylneuraminate alpha-2,8-sialyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006491">
        <property type="term" value="P:N-glycan processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009311">
        <property type="term" value="P:oligosaccharide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23963">
        <property type="entry name" value="GT29_ST8SIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1480.20">
        <property type="entry name" value="Glycosyl transferase family 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001675">
        <property type="entry name" value="Glyco_trans_29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050943">
        <property type="entry name" value="Glycosyltr_29_Sialyltrsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038578">
        <property type="entry name" value="GT29-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11987">
        <property type="entry name" value="ALPHA-2,8-SIALYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11987:SF54">
        <property type="entry name" value="ST8 ALPHA-N-ACETYL-NEURAMINIDE ALPHA-2,8-SIALYLTRANSFERASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00777">
        <property type="entry name" value="Glyco_transf_29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8040024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="241" mass="27811" checksum="B5F27B98827A81CC" modified="2023-09-13" version="1">MTVHQLLSVCQFLLPITQRTVYETVPRCSVVGNSGSILGSKCGTDVDKANFVFRCNAAPIAKYAVDAGYKSNLTTFNPSILTTRYNKFSRPEGVLRFVRDMRQYHGIVWAPCFTSSSMELCLDIMKTYTLKENKFVLGHPDHFLKIQKFWESRGHKGKISSGFFFTTIALSRCREVHLYGFWPFSKMTDRALQEVPYHYFDRLNFTSFNNLRQHNMNSEFSVLLQLHMLGVLRIHVGECHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C9M7</accession>
    <name>A0A9Q1C9M7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Copine family protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_15019</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C9M7"/>
    <dbReference type="OrthoDB" id="5855668at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010734">
        <property type="entry name" value="Copine_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052079">
        <property type="entry name" value="E3_ligase/Copine_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45751">
        <property type="entry name" value="COPINE FAMILY PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45751:SF53">
        <property type="entry name" value="VWFA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07002">
        <property type="entry name" value="Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040338218" evidence="1">
        <location>
            <begin position="22"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="2">
        <location>
            <begin position="86"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8040659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="333" mass="37135" checksum="220F31D53D8F70F3" modified="2023-09-13" version="1">MALFVFVVAVLAVLVLFTLYCEESQKLPKDSDHGLISRSESRYATLSVFEKLGLSINECNTFRTFLDRFTTYDEVVGAIKKTGMVKSDLIIGIDFSASNEWQGRKCFAVKCLHDVSNSKLLNPYQRVLTIMASTMEQFDSDHLIPVFGFGDKSCRNDSVFSMAKDGKQCKGFAEVMESYALHASSVELGGPTCLAPIIEQAIEIVEEKKSYHILLVITDGQFSGDHEDVHAIIKASEYPLSIVIVGVGDGPWDVVQEYDDKLLARHFDNLQFVPYQDVVTNCLNPETALALHILMEIPDQYAAISALGYTTQGDTYMCTEKSEEFYTNGDVYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CAR5</accession>
    <name>A0A9Q1CAR5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HYR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_12817</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CAR5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003410">
        <property type="entry name" value="HYR_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24273">
        <property type="entry name" value="FI04643P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24273:SF32">
        <property type="entry name" value="HYALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02494">
        <property type="entry name" value="HYR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50825">
        <property type="entry name" value="HYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040332450" description="HYR domain-containing protein" evidence="3">
        <location>
            <begin position="22"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="199"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="HYR" evidence="4">
        <location>
            <begin position="27"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8041891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="229" mass="24403" checksum="6448331D54D17672" modified="2023-09-13" version="1">MMNILLLLYVVSLRPLVGILGASVNVTNTSILAFSNCPNDILVISDDFNEKSLVPVVWEEPSLTGGEENVQLINNISPGSRFSWGCTHVTYTGSDASGDTDCSFQICVSNTADPYLVSGNVTIQYKSCPEGNIQVDNENSDLSVEVLPEPGLDDDDAVVTDKPPDASFFFSCNKITYTGQDASGRTATCSFLVCAGKAWATPAIIVVAIFVAVASSCIACCFIVRKCCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CB67</accession>
    <name>A0A9Q1CB67_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_13089</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8042101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CB67"/>
    <dbReference type="OrthoDB" id="6776789at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8042101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="82" mass="9459" checksum="40A1439DACF3EB33" modified="2023-09-13" version="1">MVSIPKNDKVRFGLDPKDLNKVIRREHFPMRTVEDVVFQMPNAKVVFSVLHASSGFWQISWTKKVRSYEPSIPLTRGICLSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CBR4</accession>
    <name>A0A9Q1CBR4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Beta-catenin-like protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Nuclear-associated protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_12870</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. Participates in AID/AICDA-mediated somatic hypermutation (SHM) and class-switch recombination (CSR), 2 processes resulting in the production of high-affinity, mutated isotype-switched antibodies.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the PRP19-CDC5L splicing complex composed of a core complex comprising a homotetramer of PRPF19, CDC5L, PLRG1 and BCAS2, and at least three less stably associated proteins CTNNBL1, CWC15 and HSPA8. Interacts directly with CWC15 and CDC5L in the complex. Interacts with AICDA; the interaction is important for the antibody diversification activity of AICDA. Interacts with PRPF31 (via its NLS). Interacts (via its N-terminal NLS) with KPNA1 and KPNA2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CBR4"/>
    <dbReference type="OrthoDB" id="1898821at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010467">
        <property type="term" value="P:gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:001136">
        <property type="entry name" value="Beta-catenin-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039678">
        <property type="entry name" value="CTNNBL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013180">
        <property type="entry name" value="CTNNBL1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14978:SF0">
        <property type="entry name" value="BETA-CATENIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14978">
        <property type="entry name" value="BETA-CATENIN-LIKE PROTEIN 1 NUCLEAR ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08216">
        <property type="entry name" value="CTNNBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01156">
        <property type="entry name" value="DUF1716"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Beta-catenin-like protein 1 N-terminal" evidence="11">
        <location>
            <begin position="55"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="31"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="44"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00058456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00061776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00070106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00083862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8041931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="576" mass="65934" checksum="5AFDEA5CCF774E37" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CDB2</accession>
    <name>A0A9Q1CDB2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tyr recombinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_09356</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8042575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CDB2"/>
    <dbReference type="OrthoDB" id="6769862at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="entry name" value="Intergrase catalytic core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35617:SF3">
        <property type="entry name" value="CORE-BINDING (CB) DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35617">
        <property type="entry name" value="PHAGE_INTEGRASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8042575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="103" mass="11337" checksum="9A8DF14714425B08" modified="2023-09-13" version="1">MGFEVTLEAYPKDERLCVLKTLKAYIAETQSLRDEELALFISYKKPYHRVSKDTLSRWIKMVLTNAGINTSKFKAHSTSTAATSAAAAALRQVPVCDILATAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CDG9</accession>
    <name>A0A9Q1CDG9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Tyrosine-protein kinase Srms</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_09467</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8042654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CDG9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004714">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050122">
        <property type="entry name" value="RTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416:SF611">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE TRANSMEMBRANE RECEPTOR ROR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000615">
        <property type="entry name" value="TyrPK_CSF1-R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="128"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="352"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="366"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000615-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000615-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8042654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="514" mass="59058" checksum="25C740A56685A28E" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CDJ2</accession>
    <name>A0A9Q1CDJ2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_09517</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8042695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CDJ2"/>
    <dbReference type="OrthoDB" id="2686689at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017956">
        <property type="entry name" value="AT_hook_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46791:SF5">
        <property type="entry name" value="CLR5 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46791">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00929">
        <property type="entry name" value="ATHOOK"/>
    </dbReference>
    <dbReference type="SMART" id="SM00384">
        <property type="entry name" value="AT_hook"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="557"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="173"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="198"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="211"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="284"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="310"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="355"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8042695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="662" mass="74958" checksum="CDA50644AA8F3734" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CF04</accession>
    <name>A0A9Q1CF04_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Myb/SANT-like DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_10960</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CF04"/>
    <dbReference type="OrthoDB" id="6115215at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028002">
        <property type="entry name" value="Myb_DNA-bind_5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23098">
        <property type="entry name" value="AGAP001331-PA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23098:SF16">
        <property type="entry name" value="REGULATORY PROTEIN ZESTE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13873">
        <property type="entry name" value="Myb_DNA-bind_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb/SANT-like DNA-binding" evidence="2">
        <location>
            <begin position="4"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="116"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="135"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8043731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="201" mass="22120" checksum="FAA6A481F9CB2E31" modified="2023-09-13" version="1">MAKRSYNFSEDEKVALLQFVQHHKSQLLGKAGRPGHNDDKVEERKARYWQKCADVVNGIGGKSRDWKKVRKQWFALKDKAKKLKGEMEKTGGGPKPYVSVARHLCMEVMARESLEGIRGRNTEVDPSGTATPEMSQDSSQESSIDDLAAVSPPLPPSASSLIPTTFQTAETPTTSSLLALQQQVQNLQAQVEALSNQPSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CFI6</accession>
    <name>A0A9Q1CFI6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Enteropeptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_11689</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8044272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CFI6"/>
    <dbReference type="OrthoDB" id="10009301at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051659">
        <property type="entry name" value="Serine_Protease_S1-Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24254">
        <property type="entry name" value="PROTHROMBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="CUB" evidence="7">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="148"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="186"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="148"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="192"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8044272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="210" mass="24025" checksum="B701DA4E196D4C8A" modified="2023-09-13" version="1">MSKNFPSNYPDNHRREIIFWTFDSHRVLLNFDLISTETGYDYVKVGNGNTTDEQVLLIWSGGPAENKTKVLSAGNTMWFSFKTDGSQTYKGFSGVVQAVPAESKIDCDSEFDCKNGVCIPNEYACTKYSMLCTNFFDKHGLCRGRYKKRQKESKVKSQRKTNLQQRNAKAHEDLFFSNNPSRVIRPIPFSPLTKTPTQPRPETSHTLFKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CH56</accession>
    <name>A0A9Q1CH56_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_08215</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CH56"/>
    <dbReference type="OrthoDB" id="10063284at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8045247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="214" mass="24983" checksum="391AFB5035608974" modified="2023-09-13" version="1">MLSYDFLNLLGLWDKIFIHIDLVQKKLKDSTMNFEDAVMDLKALRDHFSGAREVLISESVEEGLSLCQEWEVEVERRRRRKKRMSSENSRDTGLTAKEEMERVMKGTLDRLNREMDDRFQRLHDTDSKFGFLLNVHGLCYCTVSKELHMQENCKKLGEFYSIDIDGQQLYEEILDCRMQISGRPNTRVVAKSFFLKSSQASSHLTSSQVKSQVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CJ65</accession>
    <name>A0A9Q1CJ65_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Ubiquinone biosynthesis monooxygenase COQ6, mitochondrial</fullName>
            <ecNumber evidence="12">1.14.15.45</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">2-methoxy-6-polyprenolphenol 4-hydroxylase</fullName>
            <ecNumber evidence="12">1.14.15.46</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_08451</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">FAD-dependent monooxygenase required for two non-consecutive steps during ubiquinone biosynthesis. Required for the C5-ring hydroxylation during ubiquinone biosynthesis by catalyzing the hydroxylation of 4-hydroxy-3-(all-trans-polyprenyl)benzoic acid to 3,4-dihydroxy-5-(all-trans-polyprenyl)benzoic acid. Also acts downstream of coq4, for the C1-hydroxylation during ubiquinone biosynthesis by catalyzing the hydroxylation of 2-methoxy-6-(all-trans-polyprenyl)phenol to 2-methoxy-6-(all-trans-polyprenyl)benzene-1,4-diol. The electrons required for the hydroxylation reaction are funneled indirectly to coq6 from NADPH via a ferredoxin/ferredoxin reductase system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a 2-methoxy-6-(all-trans-polyprenyl)phenol + 2 reduced [2Fe-2S]-[ferredoxin] + O2 + 2 H(+) = a 2-methoxy-6-(all-trans-polyprenyl)benzene-1,4-diol + 2 oxidized [2Fe-2S]-[ferredoxin] + H2O</text>
            <dbReference type="Rhea" id="RHEA:81183"/>
            <dbReference type="Rhea" id="RHEA-COMP:9551"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:10858"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:62731"/>
            <dbReference type="ChEBI" id="CHEBI:84166"/>
            <dbReference type="EC" id="1.14.15.46"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a 4-hydroxy-3-(all-trans-polyprenyl)benzoate + 2 reduced [2Fe-2S]-[ferredoxin] + O2 + 2 H(+) = a 3,4-dihydroxy-5-(all-trans-polyprenyl)benzoate + 2 oxidized [2Fe-2S]-[ferredoxin] + H2O</text>
            <dbReference type="Rhea" id="RHEA:81195"/>
            <dbReference type="Rhea" id="RHEA-COMP:9514"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:10930"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:64694"/>
            <dbReference type="ChEBI" id="CHEBI:78396"/>
            <dbReference type="EC" id="1.14.15.45"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="12">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of a multi-subunit COQ enzyme complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Mitochondrion inner membrane</location>
            <topology evidence="12">Peripheral membrane protein</topology>
            <orientation evidence="12">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the UbiH/COQ6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.15.45" evidence="12"/>
    <dbReference type="EC" id="1.14.15.46" evidence="12"/>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CJ65"/>
    <dbReference type="OrthoDB" id="683240at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031314">
        <property type="term" value="C:extrinsic component of mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120538">
        <property type="term" value="F:2-methoxy-6-polyprenolphenol 4-hydroxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106364">
        <property type="term" value="F:4-hydroxy-3-all-trans-polyprenylbenzoate oxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016712">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000021">
        <property type="entry name" value="Ubiquinone biosynthesis monooxygenase COQ6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.9.10:FF:000111">
        <property type="entry name" value="Ubiquinone biosynthesis monooxygenase COQ6, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000086">
        <property type="entry name" value="Ubiquinone biosynthesis monooxygenase COQ6, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03193">
        <property type="entry name" value="COQ6_monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018168">
        <property type="entry name" value="Ubi_Hdrlase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010971">
        <property type="entry name" value="UbiH/COQ6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051205">
        <property type="entry name" value="UbiH/COQ6_monooxygenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000689">
        <property type="entry name" value="UbQ_mOase_COQ6"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01989">
        <property type="entry name" value="COQ6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01988">
        <property type="entry name" value="Ubi-OHases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43876">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS MONOOXYGENASE COQ6, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43876:SF7">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS MONOOXYGENASE COQ6, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01304">
        <property type="entry name" value="UBIH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0274">FAD</keyword>
    <keyword evidence="3 12" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 12" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="9 12 14" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="8 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="14" id="KW-0830">Ubiquinone</keyword>
    <keyword evidence="4 12" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="domain" description="FAD-binding" evidence="13">
        <location>
            <begin position="64"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding" evidence="13">
        <location>
            <begin position="385"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ8045439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="501" mass="54999" checksum="DB2931033539413A" modified="2023-09-13" version="1">MAMQTICQLHNRCLKSLATLVVCPARSLTCLAKKQRESCHHHRFQSRVRRFSQDSSSKPVTPFYDVVIVGGGMAGGALACALGSEVTLADHKILLLDHGKKPPKLTPESLGSEYSNRVCALNPQSKKLLSGIGAWSTIEKLRCKPFHRMQVWDACSDSLIAFNHPDLIQELAYIVENNVTISALTEQLEHLKQGNVDVWYETGLANLKVPVQGTIDQESWVTVTLKDMSKVKTRLLVGADGANSQVRKFGGFTMTRWTYDQSAVVATLHLAEQTENHVAWQRFLPTGPIAMLPLSETHSSLVWSTTHEEAKKLLQMSDDEFVEAVNHAFWDDSHHVASLDIVTQIFEDVLKVLQPDDSSSVRQLPPSVGGIQPGSRAKFPLGLGHAMHYVKPRIALIGDAAHRVHPLAGQGINLGFGDVSCLKDVLVKCANLGGDFGSISSLTDYETQRQRHVVPIMMVIDALKRLYSTTNPAAVLARSLGLQATNALKPLKDTIIDSASR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-04-02" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CJT9</accession>
    <name>A0A9Q1CJT9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Leucine-rich repeat-containing protein 9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_09224</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8046058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1517790at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050836">
        <property type="entry name" value="SDS22/Internalin_LRR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46652">
        <property type="entry name" value="LEUCINE-RICH REPEAT AND IQ DOMAIN-CONTAINING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46652:SF3">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14580">
        <property type="entry name" value="LRR_9"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52075">
        <property type="entry name" value="Outer arm dynein light chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="17"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="323"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1424"/>
            <end position="1506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="338"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1447"/>
            <end position="1471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8046058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1506" mass="171074" checksum="11192731176BED2A" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CKH3</accession>
    <name>A0A9Q1CKH3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_05328</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CKH3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8046596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="81" mass="9237" checksum="FCCD5F2ECC1757E7" modified="2023-09-13" version="1">MSDNLKLWYHIMMAAERTQCTRLDTFLSKTVCNPQKTNMLNNGICEVILDASLPISSAEGGFQERMKIIKPIYTVPSWKIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CL91</accession>
    <name>A0A9Q1CL91_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Short-chain collagen C4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_06286</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CL91"/>
    <dbReference type="OrthoDB" id="6086925at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005581">
        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.320">
        <property type="entry name" value="6-Phosphogluconate Dehydrogenase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051077">
        <property type="entry name" value="Ca-dependent_lectin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24024">
        <property type="entry name" value="PULMONARY SURFACTANT-ASSOCIATED PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24024:SF18">
        <property type="entry name" value="SHORT-CHAIN COLLAGEN C4-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0176">Collagen</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040436214" evidence="2">
        <location>
            <begin position="22"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="103"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="118"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8047313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="338" mass="37001" checksum="15E042081C6F1B28" modified="2023-09-13" version="1">MLALRTQCLIILSIIVAFSFGDFQLEANQEDKMNPDKLNKLQDSSHDLQPKSENGHSHLQTKSKRSRRSTGDGSLIPGNFCPGCSNSYTTYFSGQCFLCPPGSPGPRGPPGQQGIPGRDGRDGRDAMNERGDVGMMDNPDLRADFAVRSNKTGGAVYVRWGRNDCPPQSELIYEGVAGGSHYTHKGSGSNYLCMPETPLYDDPIVGTSGQNRGYIYGAEYETSTFANWNHLHNSNVYCAVCWAQTRPSLMMIPARNICPGSEWTKEYSGYLVSDYYNHGGRTEYVCMDRNAEAIPRSVHNADGSLFYPVEGRCGSSGSGLPCGPYVDGYELTCAVCTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CMK5</accession>
    <name>A0A9Q1CMK5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_06517</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CMK5"/>
    <dbReference type="OrthoDB" id="10435866at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="251"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="251"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8047498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="356" mass="40133" checksum="8C12C64221F43C23" modified="2023-09-13" version="1">MREKSFEARERGAKQIVSTSLPRSLKGFPNKERSNIERVTLSTLVTGGPEKRGKAVECLSENGTKQVKQLEKSFSLPRLDLLPSSKRNRGTSYLQGGVARGRTMTSSKHNGTEELRTGLGDAPRRGRLQRGATWHDGLRAAPLDQGKEHMDWSFLKRRTTTSPRSKEHQIVFDELTILSSGSDLQDVEKIRGTFATDIRQNQRKSGAIVSKRWCAAKTKLKAASTFKTIGDTAKRRPRDSKTTKLNIDDILLEENETETDPSNENSKSEDISKVAPKSRGWIIVKKNLPKIAEMAPKDSIDTMPMQFTNIAQIVRAKQFRMMMKRRASFIENGVFQFDEAKADLYKRYDKIGLDSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CMS4</accession>
    <name>A0A9Q1CMS4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_06871</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CMS4"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040405258" evidence="1">
        <location>
            <begin position="21"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8047781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="61" mass="6733" checksum="AFFF9DB3D07EF313" modified="2023-09-13" version="1">MAVQILTTIVILLFVNSAVSLIDFSNIGNSGFTIKGKFSVLKIYRVSKSTVHTEIKFSNKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CPT4</accession>
    <name>A0A9Q1CPT4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_01325</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CPT4"/>
    <dbReference type="OrthoDB" id="5979489at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="107"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8048850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="142" mass="15516" checksum="166CC2A915200D23" modified="2023-09-13" version="1">MPHHHHYEFRSDPRDYEHYYLNQTGHGVPVFRGGRAQRGYRLGNIVGGLFRTAVPLLKKGAKALGKEALRTGMDIAGDVLDGRSLKTSVKQRGLAAGKRVARKAVSSVGAGSGGINKKRNLSSPTTRTNSAKRAKRSLDIFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CPV4</accession>
    <name>A0A9Q1CPV4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_01091</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CPV4"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="30"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="69"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8048683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="145" mass="15119" checksum="E7C66674840AF772" modified="2023-09-13" version="1">MVEEPRSPSPDEVAHQVEQDFLAAKECEVVSGNPEVGPSVPSPSAPEAPSPSAPGEAEISTVEKDLNLSSDSESDTTMSDVGGPASEPPPKDPPTGEDWFDLCEGVESDSQLKRSASGEVSDQGVHPSMPPAPNINKKARSETVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CQ30</accession>
    <name>A0A9Q1CQ30_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Tyrosine-protein kinase CSK</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_02787</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CQ30"/>
    <dbReference type="OrthoDB" id="535509at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050198">
        <property type="entry name" value="Non-receptor_tyrosine_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24418">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8049862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="230" mass="26567" checksum="5263BC54274B6D32" modified="2023-09-13" version="1">MFLYFYTLDHLYLINEYLTCTPLDEYLTEKKLDAGSNVPFPTQRVLRYIVQILQGIEFLHTSGFLHPGLLPNKILVSNEDICKFYDFCLPKDATRRMILKKTQIHCTLNNFAPESLLRNEYTCESDVWLAANVIWYLVSSGKFIFSPVCSVTPNSKTVLTDETSWPVEYEELRNELLFQCWAPDIASRPKVHQLRRSFNEILINNQKVANADHADNGRSILSTAQNIYCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CQ95</accession>
    <name>A0A9Q1CQ95_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NACHT, LRR and PYD domains-containing protein 1b allele 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_02377</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CQ95"/>
    <dbReference type="OrthoDB" id="120976at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312">
        <property type="entry name" value="NACHT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312:SF2">
        <property type="entry name" value="NUCLEOTIDE-BINDING OLIGOMERIZATION DOMAIN-CONTAINING PROTEIN 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NACHT" evidence="1">
        <location>
            <begin position="5"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8049582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="455" mass="52496" checksum="CBAD2B00917CDFEC" modified="2023-09-13" version="1">MKKDIKHILNECNKVLIILDGFDEYPDRDSDATSDVKSIIKRDMFQEFDVVLTTRTACLPKETAPQTQRIRLTGFDDNARDEYIRKAVVGNDEGAVKAIKWQLLENPVLADLCQVPLFFVMFAHMSHESKHLEKFKSVTSFFSYMIKCFHHHLQNKMKGENVRGFESFQEDHSELDKVAFEGLCQENQQLVWGKDELCERLGEDFYHQYVSLGIIVEENVLEMEPSYTSMVASNLIQEKTEVRFYHKLFCEWYAAHYVSSYAGRRNIRFMGNDGRLVKLYHRVLGKKDISDILKDLDPFDLQYVFRFACGLNPNASSKIIEYLKNTENAHHFAILCILEKEGKIDSILHSVRDLCSSTVEIRDDHTLLLKRSTIQLLDVATNNGISISRVYLYNCYSTVDLRGGNHLQLKSNLSIPVLTALNKLDIWEQGKEITSEEFTGILQYSSKCLALKQLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CS63</accession>
    <name>A0A9Q1CS63_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_03133</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CS63"/>
    <dbReference type="OrthoDB" id="419189at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46670">
        <property type="entry name" value="ENDO/EXONUCLEASE/PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46670:SF3">
        <property type="entry name" value="ENDONUCLEASE_EXONUCLEASE_PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="175"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8050076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="238" mass="26947" checksum="363A6A0778955CCD" modified="2023-09-13" version="1">MFLTETWLKSHSDPLISDFHAAVNGYAVHQQPRLQRRGGGVAILSRCHLKADNRKTHSFKSFECLEVSFRSQSVLLRTIVVYRPPESYKNKATKNDFLADFSSLLEIVLPLPGFLLIGGDFNLHFGHHESPMSRKFQDLLESRGLSQHVADPTHVKGQTLDLLITRASDQLIDDVLARSWSLIIISFISVLIYLVHRMLSQHVLMGISVSLTVRISGHGLNPRFLIFQWTPAQMTYAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CTT7</accession>
    <name>A0A9Q1CTT7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">histidine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_03601</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>tRNA(His) + L-histidine + ATP = L-histidyl-tRNA(His) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17313"/>
            <dbReference type="Rhea" id="RHEA-COMP:9665"/>
            <dbReference type="Rhea" id="RHEA-COMP:9689"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78527"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.21"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.21" evidence="2"/>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CTT7"/>
    <dbReference type="OrthoDB" id="1906957at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004821">
        <property type="term" value="F:histidine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006427">
        <property type="term" value="P:histidyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00773">
        <property type="entry name" value="HisRS-like_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00859">
        <property type="entry name" value="HisRS_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.800:FF:000008">
        <property type="entry name" value="histidine--tRNA ligase, cytoplasmic isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000021">
        <property type="entry name" value="Probable histidine--tRNA ligase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00127">
        <property type="entry name" value="His_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015807">
        <property type="entry name" value="His-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041715">
        <property type="entry name" value="HisRS-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004516">
        <property type="entry name" value="HisRS/HisZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033656">
        <property type="entry name" value="HisRS_anticodon"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00442">
        <property type="entry name" value="hisS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11476:SF7">
        <property type="entry name" value="HISTIDINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11476">
        <property type="entry name" value="HISTIDYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13393">
        <property type="entry name" value="tRNA-synt_His"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001549">
        <property type="entry name" value="His-tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="10">
        <location>
            <begin position="9"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="78"/>
            <end position="80"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="274"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="278"/>
            <end position="279"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001549-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8050404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="459" mass="51812" checksum="D2C8A36BA2FBF14E" modified="2023-09-13" version="1">MSLLKTPKGTRDFTPKQTAIREGVFKVIIDCFKRHGAETIDTPVFELKETLMGKYGEDSKLIYDLQDQGGELLSLRYDLTVPFARYIAMNKISSMKRYHIAKVYRRDNPAMTRGRYREFYQCDFDIAGPADPMITDAECIKIVYEILSDLKLGNFVIKVNHRLLLDGLFEVCGVPQEKFRAVCSSVDKLDKTFWKDVREEMINVKGLDGQTADKIGKYVQQSGAMDLADKLMKDEVLMKCKAAKEGVEGMKLLLNFCDIFGVLDKVRFDMSLARGLDYYTGVIYEAVLEGAELKKSSDKNQEQVGVGSVAGGGRYDELVGMFAPKGTKVPCVGVSIGIERIFSIMEARVEEKNEAIRTVETEVLVASAQANMLEERLKLCNELWGSGIKAEFFYKRDPKILDQYQHAEKSQIPFLAVIGDKELKEKSVTLRNTKTREEISVPRANIASTIKTRLKAIEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1GZH2</accession>
    <name>A0A9Q1GZH2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_32863</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1GZH2"/>
    <dbReference type="OrthoDB" id="6753017at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47018">
        <property type="entry name" value="CXC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47018:SF1">
        <property type="entry name" value="TESMIN_TSO1-LIKE CXC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8027660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="129" mass="14867" checksum="BD0A680FB6A7ACCC" modified="2023-09-13" version="1">MIRIGRAMIKADRTESWESHLCAVTDILPIFAAAGHFNNLKSAYLYIQEMSKLERRNPDVYKKFHDGFHVIRRTKQYWSGLSCDLVIKQTLMRSVRGTGGLTHGSKMTEEQRTLWTMSAPIVSEYKLAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H0E6</accession>
    <name>A0A9Q1H0E6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_32864</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H0E6"/>
    <dbReference type="OrthoDB" id="6763475at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8027661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="106" mass="11949" checksum="1AE704D151870C82" modified="2023-09-13" version="1">MSRLLLIRASIDTSLLFHRFLVLSRTGDLSLEDVMKYELSPFPPSLFEDNDIFRKADKPKLAQALIAHCNNTPSCETTTDAIPQTERYVPDGGSLLHKLKWKSGDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H0Z2</accession>
    <name>A0A9Q1H0Z2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_27625</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8031032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H0Z2"/>
    <dbReference type="OrthoDB" id="407509at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33395:SF22">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33395">
        <property type="entry name" value="TRANSCRIPTASE, PUTATIVE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8031032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="108" mass="12135" checksum="8BBD31687F052116" modified="2023-09-13" version="1">MSCTKATGVDDISCKILKIAKSIIVPSLTEIINLSIRTKSVPNLWKQAKVTPIFKSGDRNDVSNYRPISVLPILSKVLERAVFDSFYSYLTKFNILCENQSGFRPKHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H2D8</accession>
    <name>A0A9Q1H2D8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Carbohydrate sulfotransferase 15</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_26971</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H2D8"/>
    <dbReference type="OrthoDB" id="526228at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050659">
        <property type="term" value="F:N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019319">
        <property type="term" value="P:hexose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052654">
        <property type="entry name" value="CS_Sulfotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15723">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15723:SF0">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sulfotransferase" evidence="1">
        <location>
            <begin position="323"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8030523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="508" mass="59331" checksum="2BC3F65BA062BB18" modified="2023-09-13" version="1">MKNTNLSSLLGKKSFCIVLSISVLIFLLIAIPNDDMPVSPIQFKNFSKSRAKEGIISNSVESVEDARFSDDVSEELFKRINGNQSTTQMQMTSILETNESAQSGLSYIRILRKNPRGKLSAMEGIEADNFRRNLLEKMNVDKDVIDNYFIEKNNIDKLKYLHDWDDVPAKLKKYAPIVFKIVPSEKDPNFKTPCWNRRSFLRCLPYFYLLGMTKSGTSDLWNKTVVHPQIINTNKETHWWSHRFENKRSSLPFSGPRGESSFLFKYTTRALSGIDMPNITLGLKSKKVLGDGSPSTLWNNRHWRRIYPGVTDGPPYINADVIRTVTPNAKFLVILREPVSRLYSSYNFFHRSSKPTPEQFHEVVVNSVAIFEECLKENGLRACTYQPVISMRSFLPNTDFTGGLYHVYLEDWFRIFSREQILVLELNDWHYHCFENLSKVYKFLELDSVPDTFIQESCNQPTRVSPKKRIARKMLDKTKRFLEEKYNPYNEKLFELLKDDRFDWRYNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H5J4</accession>
    <name>A0A9Q1H5J4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_20188</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H5J4"/>
    <dbReference type="OrthoDB" id="5973111at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8036267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="134" mass="16004" checksum="E390671020832E6B" modified="2023-09-13" version="1">MNVTLCKEVDLINPFQYKERTKERGQAWDTIADNLQKLKYCVTKWSVRDRYKLLKDQVLKKNREDAKASGISTDEVSNKPELTQIIEELVEVEKERREQQTEIREKEEKKEQDGAEMRRRALESFAETSKRYFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H5R5</accession>
    <name>A0A9Q1H5R5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Vesicular inhibitory amino acid transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_23555</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid/polyamine transporter 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H5R5"/>
    <dbReference type="OrthoDB" id="6021076at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006836">
        <property type="term" value="P:neurotransmitter transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF689">
        <property type="entry name" value="VESICULAR INHIBITORY AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0532">Neurotransmitter transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="15"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="78"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="137"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="171"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="204"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="247"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="292"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="322"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="353"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="10">
        <location>
            <begin position="1"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8033346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="389" mass="43664" checksum="5E0B74DE83B312CE" modified="2023-09-13" version="1">MFLVALPFAVLHGGYWTLLAIIVAAIITCYTGIILVECLYETNPITGQRMRVRDTYVSIAREVWGARFAARIVHTAQFIELIMTCILYLVLCGDMMYNTFGFMSESAWTSVAAFFVVPCAFLRDLKAVSRLSFGNAVAHVVINVAVVGFCVTLISNWHWSATPVLINIHHFPVSIGIIVFSYTSHIFLPSLEGSMTHRKNFRKMLYWTHGLAGLFKALFAYICFLTFASATEEVITDNLPTQGFKDLVSLCLVAKALLSYPLPYFAAAELLERAFFQGRPETVWPSCYAFDGMLHTWAVTLRVFLVVFSLILAVIIPHFALLMGLIGSFTGTMLSFVWPCWFHLKIRWDDLRWYIKVLDIAIICSGLLCCCVGIVFSSASLIAAYHEDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H5T4</accession>
    <name>A0A9Q1H5T4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_24295</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H5T4"/>
    <dbReference type="OrthoDB" id="775972at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33198">
        <property type="entry name" value="ANK_REP_REGION DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33198:SF19">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8033913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="122" mass="14173" checksum="6DB2EA00320F7A96" modified="2023-09-13" version="1">MEHFFDANEIAENEKRKAVLLSSVGPKTYKLMKTLLSPATPRDKTFTELVKLVKEHECPKPSPIVQRFKFNSRKQKESETVAEFVADLRRIAEYCEYGDKLEEMLRDCIVCGVKDFAHRDAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H6N2</accession>
    <name>A0A9Q1H6N2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Probable glycerol kinase</fullName>
            <ecNumber evidence="3">2.7.1.30</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">ATP:glycerol 3-phosphotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HOLleu_21213</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Nanhai2018</strain>
            <tissue evidence="15">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>glycerol + ATP = sn-glycerol 3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21644"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.30"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the FGGY kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.30" evidence="3"/>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H6N2"/>
    <dbReference type="OrthoDB" id="5422795at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004370">
        <property type="term" value="F:glycerol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006071">
        <property type="term" value="P:glycerol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046167">
        <property type="term" value="P:glycerol-3-phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006641">
        <property type="term" value="P:triglyceride metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07792">
        <property type="entry name" value="ASKHA_NBD_FGGY_GK1-3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000177">
        <property type="entry name" value="Glycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018483">
        <property type="entry name" value="Carb_kinase_FGGY_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018485">
        <property type="entry name" value="FGGY_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018484">
        <property type="entry name" value="FGGY_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042018">
        <property type="entry name" value="GK1-3_metazoan-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10196:SF69">
        <property type="entry name" value="GLYCEROL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10196">
        <property type="entry name" value="SUGAR KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02782">
        <property type="entry name" value="FGGY_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00370">
        <property type="entry name" value="FGGY_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00933">
        <property type="entry name" value="FGGY_KINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00445">
        <property type="entry name" value="FGGY_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0319">Glycerol metabolism</keyword>
    <keyword evidence="6 12 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbohydrate kinase FGGY N-terminal" evidence="13">
        <location>
            <begin position="12"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="Carbohydrate kinase FGGY C-terminal" evidence="14">
        <location>
            <begin position="392"/>
            <end position="548"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00043149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00052101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00071571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ8034395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="603" mass="66068" checksum="CC14F660EDDC65D6" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H6Y9</accession>
    <name>A0A9Q1H6Y9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">H(+)-transporting two-sector ATPase</fullName>
            <ecNumber evidence="2">7.1.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_22161</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ATP + H2O + 4 H(+)(in) = ADP + phosphate + 5 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:57720"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.2" evidence="2"/>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H6Y9"/>
    <dbReference type="OrthoDB" id="1676488at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046034">
        <property type="term" value="P:ATP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18111">
        <property type="entry name" value="ATP-synt_V_A-type_alpha_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1140.10:FF:000002">
        <property type="entry name" value="V-type proton ATPase catalytic subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1140.10">
        <property type="entry name" value="Bovine Mitochondrial F1-atpase, Atp Synthase Beta Chain, Chain D, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055190">
        <property type="entry name" value="ATP-synt_VA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024034">
        <property type="entry name" value="ATPase_F1/V1_b/a_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022878">
        <property type="entry name" value="V-ATPase_asu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43607:SF1">
        <property type="entry name" value="H(+)-TRANSPORTING TWO-SECTOR ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43607">
        <property type="entry name" value="V-TYPE PROTON ATPASE CATALYTIC SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22919">
        <property type="entry name" value="ATP-synt_VA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47917">
        <property type="entry name" value="C-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-1278">Translocase</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="223"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="101"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8035068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="499" mass="55876" checksum="68D488474B12D807" modified="2023-09-13" version="1">MEQGHTKIISCRSDATVVDFYWYRGLTSKQSPILKLDGRGRGGTEYGGEHFQINLNGSMIIINAKVEHESYYTFVGYFNDGNFSTSTFLVNITIAPIPPCPVISGCKPCEACNLSVSRNSGSLVCSVSGSRPSVPLNWTIPSRHGISFIKYQLNEEMGKTIDTWSTSLVLEYEITKPCGVKEVLHCEAEDNLHILESNAASVEISNDLCREDGIALRTGWKSAVIWICAVLLVLILVVVISCLVIRSRGRQRDSGYPAYLGARLASFYERAGRVKCLGNPSREGSVSLVGAVSPPGGDFSDPVTSATLGIVQVFWGLDKKLAQRKHFPSINWLISYSKYMRALDDFYDKNYPEFVPLRTKVKEILQEEEDLAEIVQLVGKGSLAETDKITLEVAKLIKDDFLQQNGYTPYDRYCPFYKTVGMLQNMIAFYDMARHSVETTAQSENKVTWAIIRENMGDIMYQLSSMKFKDPVKDGEAKIKGDFAELYENMQQSFRNLED</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H785</accession>
    <name>A0A9Q1H785_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_22060</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H785"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8034995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="241" mass="25851" checksum="AB0C41F076B7D13A" modified="2023-09-13" version="1">MHPLCVSDPVAMGALSNMSLTGHGTVGESSALSRAAARACLGKQPALQSRTSAGLVGGQPGSWGNPANSLPVHFPRTAKEARVSMGAPAVCERSCGYGCTQQHVLNRTRYSRRELRSRTSFLAHAELETGVAARKFRRCNEKSRAAARACLGKQPALQSRTSAGLVGGQPGSWGNPANSLPVHFPRTAKEARPTRDRSVPPCPHKCNGTARGRSRVDWALDDYKAGIQYYLFTSSTILYFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H7L6</accession>
    <name>A0A9Q1H7L6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Rab GTPase-activating protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_16154</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H7L6"/>
    <dbReference type="OrthoDB" id="295078at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01211">
        <property type="entry name" value="PTB_Rab6GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.750:FF:000004">
        <property type="entry name" value="Putative rab gtpase-activating protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000007">
        <property type="entry name" value="Rab GTPase-activating protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000001">
        <property type="entry name" value="TBC1 domain family member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.750">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022164">
        <property type="entry name" value="Kinesin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF9">
        <property type="entry name" value="GTPASE ACTIVATING PROTEIN AND CENTROSOME-ASSOCIATED, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12473">
        <property type="entry name" value="DUF3694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00640">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00462">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01179">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PID" evidence="4">
        <location>
            <begin position="171"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="Rab-GAP TBC" evidence="5">
        <location>
            <begin position="581"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="21"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="120"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="507"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="849"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="1008"/>
            <end position="1063"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="68"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="527"/>
            <end position="536"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8038667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1086" mass="123884" checksum="9159C3A52DE2D668" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H8S8</accession>
    <name>A0A9Q1H8S8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Extracellular serine/threonine protein kinase FAM20C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_17414</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAM20 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8036778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H8S8"/>
    <dbReference type="OrthoDB" id="8583677at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024869">
        <property type="entry name" value="FAM20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009581">
        <property type="entry name" value="FAM20_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12450">
        <property type="entry name" value="DENTIN MATRIX PROTEIN 4 PROTEIN FAM20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12450:SF22">
        <property type="entry name" value="EXTRACELLULAR SERINE_THREONINE PROTEIN CG31145"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06702">
        <property type="entry name" value="Fam20C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0464">Manganese</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="domain" description="FAM20 C-terminal" evidence="10">
        <location>
            <begin position="238"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="FAM20 C-terminal" evidence="10">
        <location>
            <begin position="479"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="346"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="366"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="366"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR624869-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR624869-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR624869-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF06702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8036778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="557" mass="64692" checksum="20BB1654E1CE6E6F" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H9W1</accession>
    <name>A0A9Q1H9W1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Sorting nexin-29</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_19029</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8038055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H9W1"/>
    <dbReference type="OrthoDB" id="428895at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17689">
        <property type="entry name" value="RUN_SNX29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004012">
        <property type="entry name" value="Run_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037213">
        <property type="entry name" value="Run_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047329">
        <property type="entry name" value="RUN_SNX29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47194:SF3">
        <property type="entry name" value="SORTING NEXIN 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47194">
        <property type="entry name" value="SORTING NEXIN-29-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02759">
        <property type="entry name" value="RUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00593">
        <property type="entry name" value="RUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140741">
        <property type="entry name" value="RUN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50826">
        <property type="entry name" value="RUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RUN" evidence="4">
        <location>
            <begin position="35"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="PX" evidence="3">
        <location>
            <begin position="733"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="248"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="367"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="415"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="630"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="705"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="584"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="250"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="294"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="319"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="415"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="438"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="707"/>
            <end position="726"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8038055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="830" mass="93010" checksum="F947ADA3EC8D427B" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HAJ6</accession>
    <name>A0A9Q1HAJ6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">BarH-like 2 homeobox protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_15641</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4 5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HAJ6"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050848">
        <property type="entry name" value="Homeobox_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000047">
        <property type="entry name" value="HTH_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24333">
        <property type="entry name" value="HOMEO BOX HB9 LIKE A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24333:SF5">
        <property type="entry name" value="VENT HOMEOBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00031">
        <property type="entry name" value="HTHREPRESSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 4 5" id="KW-0371">Homeobox</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="7">
        <location>
            <begin position="154"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="4">
        <location>
            <begin position="156"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="25"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="25"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8038266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="332" mass="38208" checksum="9B3AF13994FF9306" modified="2023-09-13" version="1">MNMDGGTRDHHKFVSENVLCFPDQHDTQTDENKPWKNKNNETNNIANDLKDESNTSRFGDVFISKKRSTFHSFLIRDILADDQVDLKEDNNNGLVSPRSVEEDFSKATKSSTEIHCLPSKKTIKRSKTKDEINDIQSTKSTIKDNEYEEIGKSKKPRKTRTAFTDHQLNLLERSFEGQKYLSVQDRLELATKLGLTDTQVKTWYQNRRNEMEAPVFFRIRFPPRSNETGGSVTPPIVPALLSLYLSNTAPSIGTTFTRDLRSSLFRSSYNRPTYYRLHYRFQITIFYEFPEHNSKTTPSFARCKEGRTRFPADCANTNASVFGFVGRLLDAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HB93</accession>
    <name>A0A9Q1HB93_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MSP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_09758</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8042882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HB93"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000535">
        <property type="entry name" value="MSP_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50202">
        <property type="entry name" value="MSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MSP" evidence="1">
        <location>
            <begin position="139"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8042882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="222" mass="24981" checksum="06104C9D5235F2BA" modified="2023-09-13" version="1">MKLTDAMTVYFVINARRFNMENHLLLVFVFSCLVSLSVCCILTDSGGCSMNGLTFGGRLTFLQTEDDMDGESSPWEGKRRFIFETPKSSHPFLNPRRGSSGWGRIWVGNSKLRGCETQTFCPPTWKRSGSDGGKSQDADTIKAPSNLKENRKSGKLIFEWDGTSDKHEYSFVRNGEESNVITNKQKVILKVNRGDTYSFKVRGFSPKKKGLFTKPINGVFHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HCK9</accession>
    <name>A0A9Q1HCK9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_14731</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HCK9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="92"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="124"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8040438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="173" mass="19694" checksum="59FE9600C2D38988" modified="2023-09-13" version="1">MPAYVKWIGTIIDQTGLKYICFNHMITTYILTSTVMFIMTLINVSSPRIATIAMSSLMSLFIVTLSIKFAYHLTHSFKTYFELTERVIDRVGLKYIFFNYVLSSCLVSILILFLITFVNTTSPRLTIGAILSLLTLLVVNLTIECAHQIIFWMHISRDPATLLDQAPPNPYLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HCV0</accession>
    <name>A0A9Q1HCV0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_15024</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HCV0"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8040663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="110" mass="12278" checksum="7C1350EB39D294AC" modified="2023-09-13" version="1">MQQPFGRDPQDLADVLDAIATEDDGRNMMPPQMTDFLSLFHVNNNDPRQTGGYVPNQDSTIHSPLALPHLPAATQKIKSLYTRRPISNRLAYCNNSNSAPSTAKRVNLMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HEG0</accession>
    <name>A0A9Q1HEG0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">LINE-1 retrotransposable element ORF2 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_13126</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8042136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HEG0"/>
    <dbReference type="OrthoDB" id="8961218at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003906">
        <property type="term" value="F:DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008311">
        <property type="term" value="F:double-stranded DNA 3'-5' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09076">
        <property type="entry name" value="L1-EN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004808">
        <property type="entry name" value="AP_endonuc_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748">
        <property type="entry name" value="AP ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748:SF26">
        <property type="entry name" value="ENDONUCLEASE_EXONUCLEASE_PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8042136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="162" mass="18604" checksum="165860AC83326D4B" modified="2023-09-13" version="1">MGFTIICLQDVHWTRDSDHIYYSEWGSDLFFSPFSSNARGVAILIDKKCVAKVLQMIYGQDGNYIILDIDIKGFTFDLVNIYGPNSGSPSFYDNVFSESFGIDCDVNIFCGDWNLVQDFVLDCDSYENVNNPNAQKKLIDLKQESHLVDPWRTLYPQTHQFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HGS0</accession>
    <name>A0A9Q1HGS0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_09066</name>
        <name evidence="1" type="ORF">HOLleu_16994</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HGS0"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8039320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8045944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="196" mass="21573" checksum="76DE27156BAB3ED5" modified="2023-09-13" version="1">MPHIERKNPIVFGAGQRSPGVTGGQTLKTLLTQYLEMRYGMRKNPIIFGGGERSSGVTGGQTLKTSLTRYLKMPHIERKNPIVFGAGQRSPGVTGGQTLKTLLTQYLEMRYGMRKNPIIFGGGERSSGVTGGQTLKTLLTRYLKMRYGMRKNPIIFGGDERSSGVTGGQTLKTLLARYLKKGSSDSSQTWYIDALY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HJH6</accession>
    <name>A0A9Q1HJH6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Endoplasmic reticulum transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HOLleu_06228</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Nanhai2018</strain>
            <tissue evidence="16">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">May play a role in anterograde transport of membrane proteins from the endoplasmic reticulum to the Golgi.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Endoplasmic reticulum membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the BCAP29/BCAP31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HJH6"/>
    <dbReference type="SMR" id="A0A9Q1HJH6"/>
    <dbReference type="OrthoDB" id="435607at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070973">
        <property type="term" value="P:protein localization to endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000011">
        <property type="entry name" value="B-cell receptor-associated protein 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008417">
        <property type="entry name" value="BAP29/BAP31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040463">
        <property type="entry name" value="BAP29/BAP31_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041672">
        <property type="entry name" value="Bap31/Bap29_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12701">
        <property type="entry name" value="BCR-ASSOCIATED PROTEIN, BAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12701:SF20">
        <property type="entry name" value="ENDOPLASMIC RETICULUM TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05529">
        <property type="entry name" value="Bap31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18035">
        <property type="entry name" value="Bap31_Bap29_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 12" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="47"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="102"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="BAP29/BAP31 transmembrane" evidence="14">
        <location>
            <begin position="1"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="Bap31/Bap29 cytoplasmic coiled-coil" evidence="15">
        <location>
            <begin position="191"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="185"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="185"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU367026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF05529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF18035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ8047263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="243" mass="27599" checksum="C382DA47A349DD9E" modified="2023-09-13" version="1">MSLQWTFVASFLYMEVIALALLMLPFIKPRTWQKLFHSRAVRAVNAYGYIYFNVFMAILIVLFLDAVRDVRKYSEAAEDVDLSVPNAEAVMNMKLFRSQRNLYIAGFAVFLWMVLSRMCTLISGTATLMASNEASIKQATGASDHAKKLMEEIEELKKQSKSGSSSNLSDTVVAAVADNEVTKELKHTQQELEKTKEELERTQNDLNSLKKQAEGVSTEYDRLLKEHADLQKKVEVEDLKKDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YBI7</accession>
    <name>A0A9Q0YBI7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_42575</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000094">
        <property type="protein sequence ID" value="KAJ8019075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YBI7"/>
    <dbReference type="OrthoDB" id="10045355at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912:SF36">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912">
        <property type="entry name" value="IP13529P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="1">
        <location>
            <begin position="3"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="1">
        <location>
            <begin position="39"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8019075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="835" mass="95660" checksum="747639E08D93FC30" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YBX4</accession>
    <name>A0A9Q0YBX4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_41716</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8019927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YBX4"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8019927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="112" mass="12559" checksum="D50AE7AB51BDCA93" modified="2023-09-13" version="1">MRHCVTLYTNRKHESLTSSPGTYNSMSEKSEGTSEGTVSVKRRKRLNGISNDEIGNVQHEEISKRKRTRGVILGKEPYCEKPETQTTKKSVESNKTAGSLLQEGACFVNLQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YC00</accession>
    <name>A0A9Q0YC00_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_41820</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8020003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YC00"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040194055" evidence="3">
        <location>
            <begin position="27"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="50"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8020003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="116" mass="13386" checksum="365635D06B961431" modified="2023-09-13" version="1">MTTNCWRPEDMRWFLLVFTMTVSATGESATPILPPTTSEEPTITLHERSAILTSASVVVFCLVTMIIMIMSLLQKRRIRKKRPNVKRTRYRNSMDRSELGLRRGREDTSLPLNRAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YCM8</accession>
    <name>A0A9Q0YCM8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_42632</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000103">
        <property type="protein sequence ID" value="KAJ8019031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YCM8"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8019031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="168" mass="19546" checksum="2DD480CC058FF8D8" modified="2023-09-13" version="1">MTLCCSLEDVKEKEKAGASSPKNRPTPPVQNSPSRKTKSSTSYVSPHFKHHLRYPSAPLKQTVRSESALLKAISSEAFIKYLEDKKKKKIEEEIQKQKRKEEREAKRKEKADTARQQTARGKGKEKRAMDRFYNCDLWFHIACTDIEDPSELEEEELEELVWECEECL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YFG7</accession>
    <name>A0A9Q0YFG7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Neuralized-like protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_39067</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="49113at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="CDD" id="cd12887">
        <property type="entry name" value="SPRY_NHR_like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000001">
        <property type="entry name" value="neuralized-like protein 4 isoform X1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037962">
        <property type="entry name" value="Neuralized"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006573">
        <property type="entry name" value="NHR_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12429">
        <property type="entry name" value="NEURALIZED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12429:SF14">
        <property type="entry name" value="NEURALIZED-LIKE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07177">
        <property type="entry name" value="Neuralized"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00588">
        <property type="entry name" value="NEUZ"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51065">
        <property type="entry name" value="NHR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NHR" evidence="2">
        <location>
            <begin position="13"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="NHR" evidence="2">
        <location>
            <begin position="234"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="NHR" evidence="2">
        <location>
            <begin position="442"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="domain" description="NHR" evidence="2">
        <location>
            <begin position="630"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="domain" description="NHR" evidence="2">
        <location>
            <begin position="835"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="domain" description="NHR" evidence="2">
        <location>
            <begin position="1045"/>
            <end position="1208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1017"/>
            <end position="1036"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8021773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1465" mass="161968" checksum="17213F626DFDD0F9" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YGN5</accession>
    <name>A0A9Q0YGN5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_43713</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000426">
        <property type="protein sequence ID" value="KAJ8018346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YGN5"/>
    <dbReference type="OrthoDB" id="6515633at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8018346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="102" mass="11333" checksum="72134C9B16D673BD" modified="2023-09-13" version="1">MASFMQHHRYYMDPKWTVLLIACARAFFTTGSVKVSGVFLGEMTHKLNTTNWVVAWAFSLQNGISLMIVCKSHFNVAAVVILISSGVGEQFGGFYAGISNWH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YH00</accession>
    <name>A0A9Q0YH00_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">TNFR-Cys domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_39202</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YH00"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001368">
        <property type="entry name" value="TNFR/NGFR_Cys_rich_reg"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50050">
        <property type="entry name" value="TNFR_NGFR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040495923" description="TNFR-Cys domain-containing protein" evidence="4">
        <location>
            <begin position="26"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="166"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="TNFR-Cys" evidence="5">
        <location>
            <begin position="55"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="repeat" description="TNFR-Cys" evidence="1">
        <location>
            <begin position="55"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="106"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="204"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="207"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="231"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8021879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="243" mass="26001" checksum="EB10B29DE1EE2DFF" modified="2023-09-13" version="1">MKGTWEKDCILLMLFIIFFLLEAQANYDERCENPGTPSPTCGSGKFKDPNGECQVCPDDQFQDAVNHICGQCKNCTKCGDGEISKDHPVLCLTYKDSTCLKVNASTTPKSDGTTTSSTSEGPFSSSSSPNTTTLEEILARTTQVSANDVTTPGSEYIQEEKHPYKIPFFVVLVLLVLVVIVLGGIIWWQRKKLKNGQHLAGNQLGQNGSGAGQNGSGAGQNGSGTGQNGSETEMTNMLANGHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YHF3</accession>
    <name>A0A9Q0YHF3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_37487</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YHF3"/>
    <dbReference type="OrthoDB" id="6283332at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8022555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="128" mass="14398" checksum="6AB89DAB4B017AB7" modified="2023-09-13" version="1">MSAKQRGISNHAHRRQKGKRVCLCAVLVKVKACGSDVPEAFWVIEERRGRRKEPFAIRSLLGWTLMGPVGPGKGAQMNVHHVRYEDDLLSQQVAKFWETDFGDSVLSEKRGSPKKIAGQGELWKILSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YHG3</accession>
    <name>A0A9Q0YHG3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_43063</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000198">
        <property type="protein sequence ID" value="KAJ8018766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YHG3"/>
    <dbReference type="OrthoDB" id="6159398at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="324"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="228"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8018766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="375" mass="42717" checksum="65660DDE33A6C704" modified="2023-09-13" version="1">MLLLLYFSDAEMTITFEADPTEYEVFEGTAVLLVYELEANDSIKIVHNGIAIVRDSVLQGSPARYTLIPPTSVNTYILRLNQVERNDSGRYFSREGIAIFKQLDLKVYYSPKSDPSCISSHDSSKYFFRDMLGEKFEFNCTVEDGNPVSEAVIYQEFKAKERRVLDVNIIRGKNEGGSLIRTLTFSSRFNSSFNTSSFTCDVSQKMPPSLKEHDYKKTCVYGPLTFQPDFNALVSPRHVTIKEGESLTLTCSTNVESAEKKWTTNVTEGVHMELKESENKAHLTLSILTGFPYDVVVAVCESSFENRNSSAKGHIKIIRKNRKYFIAIMVLFFSILIGLFGCLLFKSHFKSFSQFCTFCRRREVTEPNDEELQPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YHU0</accession>
    <name>A0A9Q0YHU0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">General transcription factor II-I repeat domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_42688</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000117">
        <property type="protein sequence ID" value="KAJ8018991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YHU0"/>
    <dbReference type="OrthoDB" id="10072451at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00084">
        <property type="entry name" value="HMG-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1460.10">
        <property type="entry name" value="GTF2I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004212">
        <property type="entry name" value="GTF2I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036647">
        <property type="entry name" value="GTF2I-like_rpt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46304">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR II-I REPEAT DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46304:SF1">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR II-I REPEAT DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02946">
        <property type="entry name" value="GTF2I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117773">
        <property type="entry name" value="GTF2I-like repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51139">
        <property type="entry name" value="GTF2I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040263853" evidence="7">
        <location>
            <begin position="21"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8018991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="231" mass="26407" checksum="A5B03F60968EBA4E" modified="2023-09-13" version="1">MAITTCTMFYSVGLLFVVFTQENKMDSRTTGYNLFCKELSSEEGVKQLASPQRFTVYAARWAALTPEGRNVFKRRAAKENTLSTRQRQQRWNKCVKKMLKIMDEMQSLQPTSGYFVLVPKDGTPRYGGTPEGNSFVTEEYETVVSGFCKGLDKQTMAKQVITVHDIRQLFNRKYAEAVGQPGKKMPYKTSSELEMEGLPEGIRLKKPQCYGTAQMRKIWDAREDIKCVLKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YHV0</accession>
    <name>A0A9Q0YHV0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_37868</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YHV0"/>
    <dbReference type="OrthoDB" id="202825at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004344">
        <property type="entry name" value="TTL/TTLL_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053317">
        <property type="entry name" value="Tubulin_polyglutamylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47113">
        <property type="entry name" value="LD09343P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47113:SF1">
        <property type="entry name" value="LD09343P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03133">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51221">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8022858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="170" mass="19604" checksum="49CDF10A9870F526" modified="2023-09-13" version="1">MRCDFVLDEDLNVYLMEVNMSPNLSSAHFEGNKHLYEQVIYNSLSVSGIARNVPASLKSRPAYVKDFQVSERDIAVAMEECANEESCDSCTEETCKLCQKCLSADEKEMLKDAYMEHLNRRSTRRVYPEPMTQEDAQNYDTGEDASLEANDRLMRAWFRAKCLQDISWCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YJW4</accession>
    <name>A0A9Q0YJW4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Low-density lipoprotein receptor-related protein 1B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_37592</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YJW4"/>
    <dbReference type="OrthoDB" id="6162427at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045197">
        <property type="term" value="P:establishment or maintenance of epithelial cell apical/basal polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007157">
        <property type="term" value="P:heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051022">
        <property type="entry name" value="Notch_Cell-Fate_Det"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057909">
        <property type="entry name" value="NRG2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24049">
        <property type="entry name" value="CRUMBS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24049:SF22">
        <property type="entry name" value="DROSOPHILA CRUMBS HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25518">
        <property type="entry name" value="NRG2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1 5" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 8" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040323837" evidence="8">
        <location>
            <begin position="30"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="500"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="148"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="9">
        <location>
            <begin position="312"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="9">
        <location>
            <begin position="389"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="9">
        <location>
            <begin position="435"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="536"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="339"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="401"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="447"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8022635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="561" mass="62034" checksum="555083FE9F9B2BA6" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YKN6</accession>
    <name>A0A9Q0YKN6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_36902</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8024232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YKN6"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8024232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="96" mass="11237" checksum="D172F0EA823462C2" modified="2023-09-13" version="1">MPSLRVFTYLFSADICRLHRLQNSVARIIFAVSRRTESSQLLISLHWLTVSKRITFKRLLFVFKSFQNQTQSYLSDCFQKYVPARQLRSCSDSPAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YKU4</accession>
    <name>A0A9Q0YKU4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_40287</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YKU4"/>
    <dbReference type="OrthoDB" id="6091153at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45823">
        <property type="entry name" value="T-SNARE COILED-COIL HOMOLOGY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45823:SF1">
        <property type="entry name" value="T-SNARE COILED-COIL HOMOLOGY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="153"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="197"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8020641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="341" mass="39261" checksum="B5ECBC9146E13E9F" modified="2023-09-13" version="1">MASTKPSDEHFSDRRKVILEEIEFLKEECERMIPLVKDTQADNLDTTSISSDRYKELPGIRTTRRDEVFQTSQGRRHHSEPYTAAEDRDHSDSVFSYRYRDHSDSTGAPQRRAESERRQQIHTDSAGMIAAGSQDNRYQSEPCVLAEDADYYQGPIPSYRDRNHTDSTGAQDAALGKSKTLKPGPHRGRSSGIADLHPTDFHHHDPVRRVRWDRQEPPESHRDDVYQRQRPREPEHWSKARPNTLPEKFDGSQPWDSYISHFESVSEINGWRQCEKARYLKASLKGDALEILADLPNTPLDFETLSTALDTRFGHAHKGPLFRAQLTMRTRERGESVQQLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YLD0</accession>
    <name>A0A9Q0YLD0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">2-phosphoxylose phosphatase 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Acid phosphatase-like protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_39647</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>3-O-[beta-D-GlcA-(1-&gt;3)-beta-D-Gal-(1-&gt;3)-beta-D-Gal-(1-&gt;4)-beta-D-2-O-P-Xyl]-L-seryl-[protein] + H2O = 3-O-(beta-D-GlcA-(1-&gt;3)-beta-D-Gal-(1-&gt;3)-beta-D-Gal-(1-&gt;4)-beta-D-Xyl)-L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:56512"/>
            <dbReference type="Rhea" id="RHEA-COMP:12573"/>
            <dbReference type="Rhea" id="RHEA-COMP:14559"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:132093"/>
            <dbReference type="ChEBI" id="CHEBI:140495"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the histidine acid phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8022227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YLD0"/>
    <dbReference type="OrthoDB" id="10262962at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050650">
        <property type="term" value="P:chondroitin sulfate proteoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006024">
        <property type="term" value="P:glycosaminoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07061">
        <property type="entry name" value="HP_HAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000560">
        <property type="entry name" value="His_Pase_clade-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050645">
        <property type="entry name" value="Histidine_acid_phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11567:SF110">
        <property type="entry name" value="2-PHOSPHOXYLOSE PHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11567">
        <property type="entry name" value="ACID PHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00328">
        <property type="entry name" value="His_Phos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="156"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00036311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00040357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00041499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8022227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="618" mass="71294" checksum="35B5814A9983EB96" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YN06</accession>
    <name>A0A9Q0YN06_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_38277</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8023172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YN06"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8023172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="84" mass="9895" checksum="305FB0E75D01BA74" modified="2023-09-13" version="1">MTFVWKDPMSHKTLYSTLQQSPSRFKCEHTFSPECKRQRSKMLYPPQTHLNVFIKKTTLESDVRNAPFGNLHIIVTRKDRSIAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YPC9</accession>
    <name>A0A9Q0YPC9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Solute carrier family 35 member G2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_33855</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8026109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YPC9"/>
    <dbReference type="OrthoDB" id="306876at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22911">
        <property type="entry name" value="ACYL-MALONYL CONDENSING ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22911:SF137">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER G2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="160"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="192"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="224"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="250"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="277"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="13"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="161"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8026109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="302" mass="32965" checksum="DBCA9D27238EF312" modified="2023-09-13" version="1">MKTVRSQLLKIRGKLFAILYAVSLGSQYFLVSTIDDSLTPYDIMFFRSFTFLLAVFFISWDGCDSYTGKDMILYIAYGFLNATGRTFAYLALSFAQVGNVTAITSNLSLPSAILGFLVLKEVITRFHVTVFVINVIGVVFVSKPPIIFQAYEGLGDVREFIGALMSLVVVITISLAATASRKLSYRRASDPPLLVFVTGVVGVIMTCLIFQFSGFHLPSSFPEITKTASVCFISLLGNIFLGIALKYETVVVVTVLMSLSLTVAYALQILILHAVPGVYELAGIALILGTNFAFLSKTQNDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YQ96</accession>
    <name>A0A9Q0YQ96_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_31491</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YQ96"/>
    <dbReference type="OrthoDB" id="10035376at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008528">
        <property type="term" value="F:G protein-coupled peptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016500">
        <property type="term" value="F:protein-hormone receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009755">
        <property type="term" value="P:hormone-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002131">
        <property type="entry name" value="Gphrmn_rcpt_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24372:SF77">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24372">
        <property type="entry name" value="GLYCOPROTEIN HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00373">
        <property type="entry name" value="GLYCHORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="201"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="235"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="280"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="326"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="372"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="428"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="460"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="10">
        <location>
            <begin position="213"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="27"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="46"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8026605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="666" mass="74937" checksum="E19D3EE2112D2FFB" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YR55</accession>
    <name>A0A9Q0YR55_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_36295</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YR55"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8023761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="96" mass="11255" checksum="11AFB33CE2CCC512" modified="2023-09-13" version="1">MDRAYCTNVLCVNCLHRTYTNRLTNMAACYSLVCKSLDLLKWPCIDKLCEKLVIFSKECKLRSNSTNRMEDCDIVLPFILIYCLKCCRFIVKLGKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-06-18" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YRC1</accession>
    <name>A0A9Q0YRC1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Mucin-5AC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_34988</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6019304at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19941">
        <property type="entry name" value="TIL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050780">
        <property type="entry name" value="Mucin_vWF_Thrombospondin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036084">
        <property type="entry name" value="Ser_inhib-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002919">
        <property type="entry name" value="TIL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014853">
        <property type="entry name" value="VWF/SSPO/ZAN-like_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001007">
        <property type="entry name" value="VWF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001846">
        <property type="entry name" value="VWF_type-D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11339">
        <property type="entry name" value="EXTRACELLULAR MATRIX GLYCOPROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11339:SF386">
        <property type="entry name" value="HEMOLECTIN, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08742">
        <property type="entry name" value="C8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01826">
        <property type="entry name" value="TIL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00094">
        <property type="entry name" value="VWD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00832">
        <property type="entry name" value="C8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00215">
        <property type="entry name" value="VWC_out"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00216">
        <property type="entry name" value="VWD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57567">
        <property type="entry name" value="Serine protease inhibitors"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51233">
        <property type="entry name" value="VWFD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040495499" evidence="4">
        <location>
            <begin position="16"/>
            <end position="1328"/>
        </location>
    </feature>
    <feature type="domain" description="VWFD" evidence="5">
        <location>
            <begin position="113"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="VWFD" evidence="5">
        <location>
            <begin position="462"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="domain" description="VWFD" evidence="5">
        <location>
            <begin position="960"/>
            <end position="1133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8024927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1328" mass="149413" checksum="632F39AD177C2D64" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YS52</accession>
    <name>A0A9Q0YS52_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Kinase</fullName>
            <ecNumber evidence="4">2.7.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_34439</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the inositol phosphokinase (IPK) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.-.-" evidence="4"/>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YS52"/>
    <dbReference type="OrthoDB" id="2573163at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000828">
        <property type="term" value="F:inositol hexakisphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032958">
        <property type="term" value="P:inositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.160">
        <property type="entry name" value="Inositol polyphosphate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005522">
        <property type="entry name" value="IPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038286">
        <property type="entry name" value="IPK_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12400">
        <property type="entry name" value="INOSITOL POLYPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12400:SF21">
        <property type="entry name" value="KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03770">
        <property type="entry name" value="IPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 6" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="374"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="405"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="376"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="405"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="422"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8024511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="485" mass="54567" checksum="0F39B923DC27E576" modified="2023-09-13" version="1">MGWQGQPRPVSEEEDSEWVCLHPFNHQVGGHCGVLLFDKTTICKPLVPAEYGIYETMSPALRKFVPKLKGWINVNIRKDTEGNTYLFTLSKKRNGARGIVEECSECQRSNAHGYPETNGGGQEKLEKEAIKNGNKSSTCAFHATSMNLRKVDQNYEVADNFCETLNNPWSVRCWKRQLWTFERAGVTKSCKYILLENLASKFKHPSILDLKIGTRGYGDDVTEEKRQAHVAKAKLSTTGTLGIRIGGMQVYQVNKDNYRRRTKHYGKSIADYNTLLSTIKEFFHNGNSICKDVLMNFIQRLKTLISVIQNEEGCRFFGCSLLILYEGGTFSQTKETSQADATRSSDLCSGDTVPLSSIGVHECAEPLMQSSDIPCDDIHNSKRESENNDLPLKKLRVKNGSITHLTKDSLTSREPENDSTNHPETSSLTNPSGSSGKDLILKVIDFPHVSYQNELTSTRYEGPDEDFLFGLNNLLEMVEKIFFSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YUB1</accession>
    <name>A0A9Q0YUB1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_31669</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YUB1"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8026746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="80" mass="9439" checksum="9ED3D35D1936CF62" modified="2023-09-13" version="1">MIDCFRVKMMIAPISWTIFLYHVDNEKPLPSPKRRLVKKKLPTEAVGFFRRETCLVEIWNILEPKFIFEGAKQSTPDKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1B8F9</accession>
    <name>A0A9Q1B8F9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Fibrinogen-like protein A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_45187</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010002043">
        <property type="protein sequence ID" value="KAJ8017421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1B8F9"/>
    <dbReference type="OrthoDB" id="6145874at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00087">
        <property type="entry name" value="FReD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.215.10:FF:000001">
        <property type="entry name" value="Tenascin isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050373">
        <property type="entry name" value="Fibrinogen_C-term_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020837">
        <property type="entry name" value="Fibrinogen_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040941">
        <property type="entry name" value="GGGWT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143">
        <property type="entry name" value="FIBRINOGEN/TENASCIN/ANGIOPOEITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143:SF444">
        <property type="entry name" value="PROTEIN SCABROUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00186">
        <property type="entry name" value="FBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00514">
        <property type="entry name" value="FIBRINOGEN_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040148896" evidence="3">
        <location>
            <begin position="22"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="4">
        <location>
            <begin position="45"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="184"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8017421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="268" mass="31556" checksum="327028306F4CC604" modified="2023-09-13" version="1">MKFLLVFIFEVGLFVSDIVKSSEFRQEEITSRTRRSTESSSYFFYQQPEYPRDCEEVYDQCDDQSADGVYLIKPESSPEPFEVYCNNSIDGGGWTVFQRRLDGSVEFYRPWNEYKNGFGFLRKDFWLGNDKISYLTNQKIYELRIDMNNVNGQPFFAKYNLFRISDDRTKYRLVGLGDYNSTSTANNDSMNHHRNQSFSTYDRDNDHSSYTNCAEYRHGAWWFNDCATSQLNGLYNAGSTDFKSIEWNSLPGGHYHLKYSEMKIRPLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BAX9</accession>
    <name>A0A9Q1BAX9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_44367</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000823">
        <property type="protein sequence ID" value="KAJ8017927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BAX9"/>
    <dbReference type="OrthoDB" id="6783964at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33480">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33480:SF1">
        <property type="entry name" value="TYR RECOMBINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8017927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="165" mass="18780" checksum="0E1BD1E920308A08" modified="2023-09-13" version="1">MIVGKLWTSSKTTVKVPELQKKKKKSMYFFGRFGALTPIRATDAMRELTQEARLHSPHLLRLTKLRKYCATLSQVFSLNESNTEWLADHLVHSLKVHKEHYRLPSSVLEKAKIAKLLMAIDAGMASNFAGKSLDEITFDGKFILLEMTTLFSNILSNKIFVTCTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BDD6</accession>
    <name>A0A9Q1BDD6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_17185</name>
        <name evidence="2" type="ORF">HOLleu_28644</name>
        <name evidence="1" type="ORF">HOLleu_37505</name>
    </gene>
    <organism evidence="1 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BDD6"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8022565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8029287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8039458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="78" mass="8998" checksum="196A8389F25852C8" modified="2023-09-13" version="1">MESSREFNFNAAFLELSRSENCLRYVPIIVVIIHRLPSLVIIRSWNQFLRYSTLQVYHCAIVNVHALPALGPDQANIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BEI2</accession>
    <name>A0A9Q1BEI2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TELO2-interacting protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_38949</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BEI2"/>
    <dbReference type="OrthoDB" id="49511at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052587">
        <property type="entry name" value="TELO2-interacting_protein_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057567">
        <property type="entry name" value="TPR_TTI1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057566">
        <property type="entry name" value="TPR_TTI1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049362">
        <property type="entry name" value="TTI1_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18460">
        <property type="entry name" value="TEL2 INTERACTING PROTEIN 1 TTI1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18460:SF3">
        <property type="entry name" value="TELO2-INTERACTING PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24176">
        <property type="entry name" value="TPR_TTI1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24181">
        <property type="entry name" value="TPR_TTI1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24173">
        <property type="entry name" value="TPR_TTI1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21547">
        <property type="entry name" value="TTI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TTI1 N-terminal TPR" evidence="1">
        <location>
            <begin position="6"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="TTI1 C-terminal TPR" evidence="2">
        <location>
            <begin position="816"/>
            <end position="1088"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF24173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8021677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1134" mass="128174" checksum="B7C519C358331785" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BFI9</accession>
    <name>A0A9Q1BFI9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Set1/Ash2 histone methyltransferase complex subunit ASH2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_37744</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BFI9"/>
    <dbReference type="OrthoDB" id="10266026at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048188">
        <property type="term" value="C:Set1C/COMPASS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15583">
        <property type="entry name" value="PHD_ash2p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12872">
        <property type="entry name" value="SPRY_Ash2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000043">
        <property type="entry name" value="Protein TRAUCO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.980.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037353">
        <property type="entry name" value="ASH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049455">
        <property type="entry name" value="ASH2-like_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053835">
        <property type="entry name" value="ASH2L-like_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10598">
        <property type="entry name" value="SET1/ASH2 HISTONE METHYLTRANSFERASE COMPLEX SUBUNIT ASH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10598:SF0">
        <property type="entry name" value="SET1_ASH2 HISTONE METHYLTRANSFERASE COMPLEX SUBUNIT ASH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21198">
        <property type="entry name" value="ASH2L-like_WH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21257">
        <property type="entry name" value="PHD_ash2p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="B30.2/SPRY" evidence="7">
        <location>
            <begin position="458"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="155"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="360"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="374"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8022757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="730" mass="82155" checksum="B771419A463CD2AD" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BGQ0</accession>
    <name>A0A9Q1BGQ0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_32083</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="5985322at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01644">
        <property type="entry name" value="RT_pepA17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005312">
        <property type="entry name" value="DUF1759"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040676">
        <property type="entry name" value="DUF5641"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008042">
        <property type="entry name" value="Retrotrans_Pao"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331:SF1">
        <property type="entry name" value="GAG-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13650">
        <property type="entry name" value="Asp_protease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03564">
        <property type="entry name" value="DUF1759"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18701">
        <property type="entry name" value="DUF5641"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05380">
        <property type="entry name" value="Peptidase_A17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="8">
        <location>
            <begin position="1442"/>
            <end position="1630"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="98"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="102"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8027051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1749" mass="199708" checksum="99184B68F2AD3DF6" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BH85</accession>
    <name>A0A9Q1BH85_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Deleted in malignant brain tumors 1 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_31288</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BH85"/>
    <dbReference type="OrthoDB" id="536948at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000006">
        <property type="entry name" value="neurotrypsin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000016">
        <property type="entry name" value="Scavenger receptor cysteine-rich protein type 12"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19331:SF465">
        <property type="entry name" value="EGG PEPTIDE SPERACT RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19331">
        <property type="entry name" value="SCAVENGER RECEPTOR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00530">
        <property type="entry name" value="SRCR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00258">
        <property type="entry name" value="SPERACTRCPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="460"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="12">
        <location>
            <begin position="20"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="12">
        <location>
            <begin position="135"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="12">
        <location>
            <begin position="245"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="12">
        <location>
            <begin position="352"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="498"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="91"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="160"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="173"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="204"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="270"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="283"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="314"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="422"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8026465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="540" mass="59880" checksum="4BAF5149502DF2C5" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BJZ1</accession>
    <name>A0A9Q1BJZ1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_30180</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BJZ1"/>
    <dbReference type="OrthoDB" id="10040781at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8028053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="161" mass="18716" checksum="9A7E07C855AFD664" modified="2023-09-13" version="1">MQHLKPSDAALEARDKLNQTTNRSLNTHSFDRAWQRVYGAKEYRPSWVGGLSQPRPRPAPSYRQKPILTVNDEPIVENLDEITANLPREEKKKVKEFYDLQVQGIPTEHRCKRAHEPLLISGKVVPREHELGRWREMITTDEVDEVTGEPRSTKRSAYGYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BKA6</accession>
    <name>A0A9Q1BKA6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NudC domain-containing protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_30364</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BKA6"/>
    <dbReference type="OrthoDB" id="515366at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.740">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007052">
        <property type="entry name" value="CS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037898">
        <property type="entry name" value="NudC_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12356">
        <property type="entry name" value="NUCLEAR MOVEMENT PROTEIN NUDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12356:SF18">
        <property type="entry name" value="NUDC DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04969">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51203">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CS" evidence="1">
        <location>
            <begin position="13"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8028196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="152" mass="17426" checksum="5E9D0EBCEC13370F" modified="2023-09-13" version="1">MSNFDEKSGIVPCRTPWGQWYQTMEDVVVEVSVPEGTTSKEIKVDFKPKQLTCKVRNEEIKGELYGSVIVDDCVWSLEDRKTVRIMLVKSRRSADNCWPSLFVDQYKADPFTFDQMEKKMTLERFQTENPGFDFSGADITGSYSGGGPQWKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BMV9</accession>
    <name>A0A9Q1BMV9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_29124</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BMV9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8029673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="81" mass="8887" checksum="A1BC9AFD6E6723F4" modified="2023-09-13" version="1">MRYGKRKNSVVFGGGQRSSGVTGGQTLKILLTQYLKMCYGKRKNPIIFGGGQRSSGVNGGQTLKTMLTRYLEIGSLDRFHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BNL4</accession>
    <name>A0A9Q1BNL4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein MAK10 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_29545</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MAK10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BNL4"/>
    <dbReference type="OrthoDB" id="269405at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031417">
        <property type="term" value="C:NatC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057983">
        <property type="entry name" value="NAA35-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007244">
        <property type="entry name" value="Naa35_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057982">
        <property type="entry name" value="TPR_NAA35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21373">
        <property type="entry name" value="GLUCOSE REPRESSIBLE PROTEIN MAK10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21373:SF0">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 35, NATC AUXILIARY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04112">
        <property type="entry name" value="Mak10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25789">
        <property type="entry name" value="TPR_NAA35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAA35-like N-terminal" evidence="7">
        <location>
            <begin position="56"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="NAA35-like TPR repeats" evidence="8">
        <location>
            <begin position="357"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="463"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00030494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF25789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8029987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="747" mass="85163" checksum="6599D594CB9857A2" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BQE7</accession>
    <name>A0A9Q1BQE7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SCAN box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_27421</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BQE7"/>
    <dbReference type="OrthoDB" id="10051775at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF1">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8030880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="160" mass="18384" checksum="D2BD099B22FF84BD" modified="2023-09-13" version="1">MHVAESCDYKKLKSALLNKYALTTDGYRSKFRSAKCGVSETYVQFIDRLKVYLNRWIESIGTNKNYKDLFDLILQEQVINSIDKELAIFLKEKGLKCSSDMATFADRYTEARVTYHGGERKSGSTDKGKLNFKFTQSQNSQTKSETSGTQKYKERSCFVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BS82</accession>
    <name>A0A9Q1BS82_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_24873</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BS82"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040375996" description="Ig-like domain-containing protein" evidence="3">
        <location>
            <begin position="16"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="379"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="19"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="332"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="333"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="344"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8031624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="442" mass="48384" checksum="374AE215233CEDC5" modified="2023-09-13" version="1">MLPLLVFCFVLPCHCYRTPVTSDESCIDTIHVPSMQGQNVTITCNTSNTLDCCTRGLWRGKGKKWLIPDNCQDCGDSFSVTHNISNNINILQISNISKSDVGIYICQCDVYQNMEGCSSSNGRMNFGCYNVTIPDCQLQYEVNGVRTNLYGSSGTRSKAVVDVRENDTISVHCVVGSDKTDCSKMKKESSITVERSHNGRKFRCKCSKDNVTCNIVIVLNVTSNRTSTSIAIPVTTNDVIINNKTFDWTSTRKPKTNILTAVTNSIEASSLTSTNGLSSVSSHVLIDNSSKPILHTVDGSSVSEGFASAVSTTMNKVRSFPSTSNSANIISRSNDEFHHERTSNELSTTSKASDSSTVTPTGTQMVSISTQSYFPANDIYVIILSCFIVGLGAVVILLCLGIFLCRARRQCIQVHNDKTEEWVNPIYESSDNKIQLKAAPNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BTE6</accession>
    <name>A0A9Q1BTE6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_25966</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BTE6"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8032440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="98" mass="11227" checksum="3FAAB2679F264071" modified="2023-09-13" version="1">MTIGIKRSGQTKNKVEFTLEVKDLCHGCHVRRRKHILLTMREKTKKRYNRGLKTLVLTGVKPADYKCRKMAPPTVVSKHSSHASRTYAKPKISPSVLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BW65</accession>
    <name>A0A9Q1BW65_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Interferon-induced very large GTPase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_21261</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class dynamin-like GTPase superfamily. Very large inducible GTPase (VLIG) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1597724at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030383">
        <property type="entry name" value="G_VLIG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057365">
        <property type="entry name" value="URGCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052986">
        <property type="entry name" value="VLIG_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14819:SF25">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_52, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14819">
        <property type="entry name" value="GTP-BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25496">
        <property type="entry name" value="URGCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25683">
        <property type="entry name" value="URGCP_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Up-regulator of cell proliferation-like" evidence="2">
        <location>
            <begin position="86"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="VLIG-type G" evidence="3">
        <location>
            <begin position="400"/>
            <end position="699"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8034428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1660" mass="191689" checksum="0B3A9BC45571A2AA" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BY60</accession>
    <name>A0A9Q1BY60_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_21603</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BY60"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8034671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="68" mass="7957" checksum="42FB3C2ED7EEAFA9" modified="2023-09-13" version="1">MISQAYHRQLKGIGFTCAHTRKARENFTLVMDIFTSFCKPVSHNWKKDRGRLDLNQLVGNWDVKPHLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BZV1</accession>
    <name>A0A9Q1BZV1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_20454</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BZV1"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8036468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="124" mass="14055" checksum="3E451A427D5BCE63" modified="2023-09-13" version="1">MAHIERKNPIVFGGGQRSSGVTGGQNLKTFFTRYLKMQCGERKNPSVFGGGQRSSGVTRGQNLKTLFTRYLKMRFGQRKNPIIFGGGQMSSGVTRGQNLKTLFTRYLKLESLDRFHAWYVDALW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C053</accession>
    <name>A0A9Q1C053_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cullin-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_20048</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 4 5">Belongs to the cullin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C053"/>
    <dbReference type="OrthoDB" id="27073at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000161">
        <property type="entry name" value="Cullin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000007">
        <property type="entry name" value="Cullin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000011">
        <property type="entry name" value="Cullin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000019">
        <property type="entry name" value="Cullin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000023">
        <property type="entry name" value="cullin-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1030.10:FF:000001">
        <property type="entry name" value="Putative Cullin-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1310.10">
        <property type="entry name" value="Cullin Repeats"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1030.10">
        <property type="entry name" value="Ring Box Chain A, domain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045093">
        <property type="entry name" value="Cullin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059120">
        <property type="entry name" value="Cullin-like_AB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016158">
        <property type="entry name" value="Cullin_homology"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036317">
        <property type="entry name" value="Cullin_homology_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001373">
        <property type="entry name" value="Cullin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016159">
        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11932">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00888">
        <property type="entry name" value="Cullin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26557">
        <property type="entry name" value="Cullin_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00182">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75632">
        <property type="entry name" value="Cullin homology domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50069">
        <property type="entry name" value="CULLIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Cullin family profile" evidence="6">
        <location>
            <begin position="416"/>
            <end position="643"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8036161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="682" mass="78912" checksum="5DDF92E79D667511" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C3N0</accession>
    <name>A0A9Q1C3N0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Dehydrogenase/reductase SDR family member 7B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_18332</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Putative oxidoreductase.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C3N0"/>
    <dbReference type="OrthoDB" id="5307821at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05332">
        <property type="entry name" value="11beta-HSD1_like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004825">
        <property type="entry name" value="PRK06181.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44196">
        <property type="entry name" value="DEHYDROGENASE/REDUCTASE SDR FAMILY MEMBER 7B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44196:SF1">
        <property type="entry name" value="DEHYDROGENASE_REDUCTASE SDR FAMILY MEMBER 7B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="154"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00037096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8037504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="311" mass="33705" checksum="36B896611A6A08E6" modified="2023-09-13" version="1">MSILQHNFQGTPAVLAAAVFGGLGLLWLYRKLRRRQALASLHGKVVLITGASSGIGEACAHAFHQVGCKVILTARRLNELERVKTEIIAQSAKGKTDAPKVLQLDVSDLESIPEKMKEALAFHGRIDVLVNNAGMSYRGSVKDTKMEVHQKVMLVNYFGSVAVTTAILPHMISKKSGTIIAVSSIQGKLAVPFRTAYTASKFAMQAFFDSLRAEVAQYNIRVGVSSPSYVQTNISVNAVRGDNSQYGVTDANIAKGMSAEYLADEIVLQAATGEEDVIVGPFIHRLAPHLNVLFPGLMRMVMKLRAKKGIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C4D0</accession>
    <name>A0A9Q1C4D0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14 25">Angiotensin-converting enzyme</fullName>
            <ecNumber evidence="25">3.4.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="29" type="ORF">HOLleu_15887</name>
    </gene>
    <organism evidence="29 30">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="29">Nanhai2018</strain>
            <tissue evidence="29">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>Release of a C-terminal dipeptide, oligopeptide-|-Xaa-Yaa, when Xaa is not Pro, and Yaa is neither Asp nor Glu. Thus, conversion of angiotensin I to angiotensin II, with increase in vasoconstrictor activity, but no action on angiotensin II.</text>
            <dbReference type="EC" id="3.4.15.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="25">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="25">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 24 25">Belongs to the peptidase M2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="29">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.-.-" evidence="25"/>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10029630at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004180">
        <property type="term" value="F:carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008241">
        <property type="term" value="F:peptidyl-dipeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06461">
        <property type="entry name" value="M2_ACE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1370.30:FF:000004">
        <property type="entry name" value="Angiotensin-converting enzyme"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1370.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1370.20">
        <property type="entry name" value="Oligoendopeptidase f, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024731">
        <property type="entry name" value="NELL2-like_EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042088">
        <property type="entry name" value="OligoPept_F_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001548">
        <property type="entry name" value="Peptidase_M2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10514">
        <property type="entry name" value="ANGIOTENSIN-CONVERTING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10514:SF24">
        <property type="entry name" value="ANGIOTENSIN-CONVERTING ENZYME 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12947">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01401">
        <property type="entry name" value="Peptidase_M2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00791">
        <property type="entry name" value="PEPDIPTASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52011">
        <property type="entry name" value="PEPTIDASE_M2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 25" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="11 20 24" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2 23" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="12 16 25" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 25" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="26" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 19 25" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 25" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4 25" id="KW-0645">Protease</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 27" id="KW-0732">Signal</keyword>
    <keyword evidence="26" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="26" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9 19 25" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="27">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040475967" description="Angiotensin-converting enzyme" evidence="27">
        <location>
            <begin position="23"/>
            <end position="1339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="1320"/>
            <end position="1338"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="28">
        <location>
            <begin position="608"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="28">
        <location>
            <begin position="1273"/>
            <end position="1313"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor 1" evidence="15">
        <location>
            <position position="375"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor 2" evidence="21">
        <location>
            <position position="375"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor 1" evidence="15">
        <location>
            <position position="504"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor 2" evidence="21">
        <location>
            <position position="504"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor 2" evidence="17">
        <location>
            <position position="1040"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor 2" evidence="17">
        <location>
            <position position="1169"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>chloride</name>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="374"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="374"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="378"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="378"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="402"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="402"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="513"/>
        </location>
        <ligand>
            <name>chloride</name>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="16">
        <location>
            <position position="63"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20 24">
        <location>
            <begin position="142"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20 24">
        <location>
            <begin position="343"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="529"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="24">
        <location>
            <begin position="807"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="24">
        <location>
            <begin position="1008"/>
            <end position="1026"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00036868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00039858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR601548-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR601548-10"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR601548-11"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR601548-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR601548-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR601548-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR601548-6"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR601548-8"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="RuleBase" id="RU361144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="EMBL" id="KAJ8038456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1339" mass="153011" checksum="8CBA179CD8C80342" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C4Z0</accession>
    <name>A0A9Q1C4Z0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Helicase SKI2W</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_16239</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
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    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAJ8038482.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
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    <sequence length="4372" mass="488261" checksum="65EEF967745BCEB4" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C5N0</accession>
    <name>A0A9Q1C5N0_HOLLE</name>
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            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
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        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
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        <lineage>
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        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
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        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="KAJ8038630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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            <dbReference type="EMBL" id="KAJ8038630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
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                <person name="Chen T."/>
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            <strain evidence="2">Nanhai2018</strain>
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        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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            <dbReference type="EMBL" id="KAJ8039531.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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    <protein>
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            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
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                <person name="Chen T."/>
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        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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        </location>
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        <source>
            <dbReference type="EMBL" id="KAJ8040147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="145" mass="17827" checksum="C95F5C3EB18C2EE4" modified="2023-09-13" version="1">MPFNLDKFVASPSVEELDSLKKSEIVKVAKHYGIEFQPLMRKDEIKRYVLEYLVDESILPSTVLETAITVPTDNTFELKRLEMEMNKEIRLKEMEREREREEREREERERERKKEREEREMQMQKEKEEREMQMQREEKAKSKRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C887</accession>
    <name>A0A9Q1C887_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">UDP-glucuronosyltransferase 2B23</fullName>
        </submittedName>
    </protein>
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        <name evidence="6" type="ORF">HOLleu_14439</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="KAJ8040210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome 6"/>
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        <property type="term" value="F:UDP-glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050271">
        <property type="entry name" value="UDP-glycosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043">
        <property type="entry name" value="EG:EG0003.4 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043:SF145">
        <property type="entry name" value="FI06409P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="1541"/>
            <end position="1565"/>
        </location>
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        <location>
            <begin position="1273"/>
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        <location>
            <begin position="445"/>
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        <location>
            <begin position="772"/>
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        <location>
            <begin position="1112"/>
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        </location>
    </feature>
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        <location>
            <begin position="1194"/>
            <end position="1217"/>
        </location>
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        <location>
            <begin position="1218"/>
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        <location>
            <begin position="1294"/>
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    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAJ8040210.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1585" mass="175117" checksum="BE668BCD13F513C1" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C8T8</accession>
    <name>A0A9Q1C8T8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">S-phase kinase-associated protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_15186</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C8T8"/>
    <dbReference type="OrthoDB" id="2095648at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318">
        <property type="entry name" value="PARTNER OF PAIRED, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="110"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8040787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="440" mass="50489" checksum="22CCE8142C858ACF" modified="2023-09-13" version="1">MKSPKADATSFDNLAYAQRYVVMEKSSTRCVWDVDWKETQEIYRDIGVQELDPDKPEKQTKRILRKSQKRKQEFQTPENQQEQPTKKSLSSTKNGCKCQLFKTYPENVVTDKFETLSDEIMLCILKWLPRRQLAKCARISKRWKQLCQDESLWKRIDLSECKFSSETLGPVLLRTLFAAKLARCTIKGPLFEPKFDAENYCPEFMIEYLDLSMCNADSELVGDIVGRCPYLVKLSLESCEVTEDMLASISLQRSLEELNLCMAQNISGKMLIMAMEMSERLKSLNLAWLNLSRNVISSLVKVLPQTLERLNLSGCRESLQDADVENLSARCPNLVELDLSDGCALTGNIFVYLESIRNLQHLALSRCYRIPNPLLLMDHLKQLRSLKAVELFGLITEHDLNTLCKGLAPVRINTHPFSSISRPTMSVSKRSYVWEVNCRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CAZ6</accession>
    <name>A0A9Q1CAZ6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_12267</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CAZ6"/>
    <dbReference type="OrthoDB" id="408457at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8041451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="593" mass="67890" checksum="AFFEB9868F0D8A15" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CCL7</accession>
    <name>A0A9Q1CCL7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_10128</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CCL7"/>
    <dbReference type="OrthoDB" id="5978043at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="7">
        <location>
            <begin position="211"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="7">
        <location>
            <begin position="231"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="168"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="168"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8043158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="373" mass="41864" checksum="62CAFB1D63CAAEEF" modified="2023-09-13" version="1">MAHFGKVTEFIAGKEIWSQYVERLEHFFAANGITDKAKMQSILLCSIGPDAYKLLRNLVSPAKPGEKSYKELVAIMAKHQHPTPSEIVQRCKFNSRQRKQGESIATYVAELRAIAEFCNYGDKLEEMLRDRLVCGVNDDRIQCSLLKRSELTLEKALEIAISSETASHNATDLKKASQQSSPSTSANVEGVVKSIPKGKRTRKSTAKSTTCYRCGGRNHDSKDCWFKDSICRSCGKKGHIEKVCRSKKIKQVIKPVTDAHRSETRQSENEDYDLFMLQARKGGPLTVEVNINKIPTVMEVDTGASLSIMTEAEFRKSWPKVTVRQSEVPLKTYTGEPLAIVGMATVNVQYKNQRNKDFVSSKEMDQVYLVEIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CD75</accession>
    <name>A0A9Q1CD75_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_13585</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8042515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CD75"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040403489" evidence="2">
        <location>
            <begin position="20"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="148"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8042515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="168" mass="18181" checksum="82CA932A433BE44B" modified="2023-09-13" version="1">MKSLVCAIFLLAAVFDAKAITCYSISETDCTAYDSRLLSACQDLEAGNTRYTETCPSSSDSCYYSTFYISEPGLYRLTARIGGCEYGAINGCYSRSELSSIDPSIEVVLDDLEDTLGSNIQGLQACFCNSNLCNTGLSNNSLLLQTNYMIVGLLALLALLPVIEFTVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CD79</accession>
    <name>A0A9Q1CD79_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">IgGFc-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_10126</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6236007at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd19941">
        <property type="entry name" value="TIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022041">
        <property type="entry name" value="Methyltransf_FA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050780">
        <property type="entry name" value="Mucin_vWF_Thrombospondin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036084">
        <property type="entry name" value="Ser_inhib-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025615">
        <property type="entry name" value="TILa_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014853">
        <property type="entry name" value="VWF/SSPO/ZAN-like_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001846">
        <property type="entry name" value="VWF_type-D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11339">
        <property type="entry name" value="EXTRACELLULAR MATRIX GLYCOPROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11339:SF373">
        <property type="entry name" value="VWFD DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08742">
        <property type="entry name" value="C8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12248">
        <property type="entry name" value="Methyltransf_FA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12714">
        <property type="entry name" value="TILa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00094">
        <property type="entry name" value="VWD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00832">
        <property type="entry name" value="C8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00216">
        <property type="entry name" value="VWD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57567">
        <property type="entry name" value="Serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51233">
        <property type="entry name" value="VWFD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040427312" evidence="3">
        <location>
            <begin position="21"/>
            <end position="1303"/>
        </location>
    </feature>
    <feature type="domain" description="VWFD" evidence="5">
        <location>
            <begin position="349"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="domain" description="VWFD" evidence="5">
        <location>
            <begin position="761"/>
            <end position="948"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="4">
        <location>
            <begin position="1042"/>
            <end position="1094"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="4">
        <location>
            <begin position="1240"/>
            <end position="1299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8043157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1303" mass="146681" checksum="E5185119C673B966" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CDV0</accession>
    <name>A0A9Q1CDV0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Retinol dehydrogenase 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_09539</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8042709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CDV0"/>
    <dbReference type="OrthoDB" id="191139at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43157:SF31">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS F PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43157">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS F PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8042709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="292" mass="32097" checksum="D4C534DF3B2D7A5B" modified="2023-09-13" version="1">MEQIGKCTCSKRMDGKTIIVTGANSGIGKETARALANRGARVILACRDRTRGRETELELRQQTGNQSIIFMHLDLSSFDSIRDFANEFLTMEQNLHVLVNNAGVINPGNCHTAEGYELSFGVNHLGHFLLTSLLMDRLKESAPSRVINVSSIAYRLGVIDFDKLKESKGRVKSYARSKLANILFTRMLAAKMEGTGVTAFSLHPGSIDTGIKRNWSSWLKRMAPLITMFLKSPIEGARASIHCAVADDIEAHNGGYFEGCKAKKLTPAACDDDLALALWVKSLEMTGMNTEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CEQ5</accession>
    <name>A0A9Q1CEQ5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">B-cell CLL/lymphoma 7 protein family member B-B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_10753</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BCL7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CEQ5"/>
    <dbReference type="OrthoDB" id="5989898at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006804">
        <property type="entry name" value="BCL7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12767">
        <property type="entry name" value="BCL7 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12767:SF9">
        <property type="entry name" value="BCL7-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04714">
        <property type="entry name" value="BCL_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="49"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="55"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="87"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="130"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="192"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="206"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8043585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="231" mass="25761" checksum="CCAA3935372FFC01" modified="2023-09-13" version="1">MLVSGRSSRAETRSRAKDEIKRVMQAVDRVRKWEKKWVSVGDTSLQIFKWVPIAKSREEQKPKDEGEKLENGQEDDSKDGGNSGKDQYLKEQNKDDKSGEKQEMPKHRDIAALISEESRAGWSDEDSRGDSNFSSDNEDGSSTTVAQSEGGSGRNSTIPPEHLADSLDAKSISDETKEGDTSRRKRSLGMIESSQDSIGLSNESSMDTKGDDEPPIKQRHMSDDYKTSKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CF68</accession>
    <name>A0A9Q1CF68_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">SEC14-like protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_11091</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CF68"/>
    <dbReference type="OrthoDB" id="1434354at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.680">
        <property type="entry name" value="GOLD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036598">
        <property type="entry name" value="GOLD_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051064">
        <property type="entry name" value="SEC14/CRAL-TRIO_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23324">
        <property type="entry name" value="SEC14 RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23324:SF83">
        <property type="entry name" value="SEC14-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101576">
        <property type="entry name" value="Supernatant protein factor (SPF), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="1">
        <location>
            <begin position="23"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8043821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="326" mass="37904" checksum="C08DC5664389F528" modified="2023-09-13" version="1">MLRDHFEFRIKYDCDNIQTSWKPPEVLDKYMIGGLFGEDRDGHPVWYDPYGCLDTKGIFYSCTRQDFVKYKVLACETIQEVLREQSKKMGHRIETINVVFDLEHASWSHLWRPFTDRYSHVLTAYEANYPETLRRGIIINAPKMFTLGFQLIKPFLSEETKQKVMILGSNYKDKLQKFIHPDQLPVHYGGKAVDEYGDAKCRSKICFGGKIPSSYYLNQETNNKYDSMSSCVVPHGKYEEFLYDVTEPGSCLKWDFRTKSDNVTFGIFLEENGSEHPKDVVPLVKCESHIVPETGEVICEKIGRCKYTHLVRKKCQQRLQLSLASI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CFF6</accession>
    <name>A0A9Q1CFF6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_10962</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CFF6"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="97"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8043733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="108" mass="12226" checksum="83F39CD65D829329" modified="2023-09-13" version="1">MSRLTKAEQNVKSAKGHLDDFSEDKTTTVLNDPQRALEDDQEEIEHIQPQQVNTDSCQEGEEQGDSTSEQTSIPTQKDTIVEDPPFIRPKIGQKVQYRGEDGESHRET</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CFJ1</accession>
    <name>A0A9Q1CFJ1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">G patch domain-containing protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_11340</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8044005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CFJ1"/>
    <dbReference type="OrthoDB" id="5842926at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039536">
        <property type="term" value="P:negative regulation of RIG-I signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032480">
        <property type="term" value="P:negative regulation of type I interferon production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000467">
        <property type="entry name" value="G_patch_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040341">
        <property type="entry name" value="GPATCH3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14390">
        <property type="entry name" value="G PATCH DOMAIN CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14390:SF2">
        <property type="entry name" value="G PATCH DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01585">
        <property type="entry name" value="G-patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00443">
        <property type="entry name" value="G_patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50174">
        <property type="entry name" value="G_PATCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G-patch" evidence="2">
        <location>
            <begin position="425"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="276"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="442"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="276"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="286"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="461"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8044005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="518" mass="58898" checksum="65B0D1C5FB50DB0B" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CGZ6</accession>
    <name>A0A9Q1CGZ6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">E3 ubiquitin-protein ligase CHFR</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_07039</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CHFR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CGZ6"/>
    <dbReference type="OrthoDB" id="5330228at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070936">
        <property type="term" value="P:protein K48-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22663">
        <property type="entry name" value="FHA_RNF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16535">
        <property type="entry name" value="RING-HC_RNF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15067">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15067:SF4">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FHA" evidence="11">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="12">
        <location>
            <begin position="462"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="13">
        <location>
            <begin position="596"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="108"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="534"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="227"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="117"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="534"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="556"/>
            <end position="566"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ8044314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="612" mass="70173" checksum="E450277439958BAB" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CH45</accession>
    <name>A0A9Q1CH45_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Gamma-aminobutyric acid type B receptor subunit 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_07123</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CH45"/>
    <dbReference type="OrthoDB" id="2150267at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038039">
        <property type="term" value="C:G protein-coupled receptor heterodimeric complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004965">
        <property type="term" value="F:G protein-coupled GABA receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007214">
        <property type="term" value="P:gamma-aminobutyric acid signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002455">
        <property type="entry name" value="GPCR3_GABA-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10519:SF20">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 156-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10519">
        <property type="entry name" value="GABA-B RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Receptor ligand binding region" evidence="9">
        <location>
            <begin position="46"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8044389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="266" mass="29792" checksum="C5C7562A60E6E9D0" modified="2023-09-13" version="1">MSGLQFGILQVPVINAQNVTATTAGNIPLYISGMFSMTNGWDGSGVLPAVELALEQINERTDILEGYNLQLVWNDSQCVAGKAPRILFDHINNEPTKLIILGAACSPNTQAIAVAAHYWNLLTVIVTEIVDKEAKNQVENLKEQQVRIIFLMSFEVIARKVFCETPSEFSVLMKRRIATWPKYRYVTHNNYMAYAFDAMWAIGLMLNNTVTRLREGATKGPVNKLEDFSYNDSGRDLSKIFFEELARTNFFGASVSADLVRLISQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CH97</accession>
    <name>A0A9Q1CH97_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Synaptotagmin-14</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_07547</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CH97"/>
    <dbReference type="OrthoDB" id="5978493at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08389">
        <property type="entry name" value="C2A_Synaptotagmin-14_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08408">
        <property type="entry name" value="C2B_Synaptotagmin-14_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000062">
        <property type="entry name" value="synaptotagmin-14 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043541">
        <property type="entry name" value="SYT14/14L/16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46129">
        <property type="entry name" value="SYNAPTOTAGMIN 14, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46129:SF2">
        <property type="entry name" value="SYNAPTOTAGMIN 14, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="2">
        <location>
            <begin position="141"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="2">
        <location>
            <begin position="298"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="268"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="87"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="276"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8044723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="441" mass="48860" checksum="A4585D9B958D1959" modified="2023-09-13" version="1">MCLQILKWLHLFSHGANITPMYIRKSARMTPSVSQDPETPNGKVSHGPEDETDSKSEEFHFPTAVMMFLGILTEKQRDNPSAKSDDASAADSDQAAGGQEERGIEGEILDDSQLPTEPVESLPLNDDGSAAEPEEAAKGPSVGEVEVAFQYSGENRRMNITILQCHNLPSKDKGGASSYRLRLVLYPAKRQRAKTRIRDGDNPVFKELFRFSRIYPHELVSSSVRLRLYGAERMRKERLIGEGTVKFSALNVSKKQVIRLRLKPQSEIGGDDTPYSSMSDLSQSESSSSLQSLQHGGGLPELLAGLSYNSTTGRLAVEIIKGSHFKQLAAGRAPDSYVKVAVMSSSGFEMAKSKSSVRRGQPNPTFKETFIFQIAQFQLNEVTLMFTVFNKKGMKRKNALGWFSMGLSNTSEEELAHWNEMQSSNGQQVCRWHALLESKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CHB8</accession>
    <name>A0A9Q1CHB8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9 11">Adenylosuccinate synthetase</fullName>
            <shortName evidence="9">AMPSase</shortName>
            <shortName evidence="9">AdSS</shortName>
            <ecNumber evidence="9 11">6.3.4.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">IMP--aspartate ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_07423</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Plays an important role in the de novo pathway and in the salvage pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP.</text>
    </comment>
    <comment type="function">
        <text evidence="11">Plays an important role in the de novo pathway of purine nucleotide biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9 11">
            <text>IMP + L-aspartate + GTP = N(6)-(1,2-dicarboxyethyl)-AMP + GDP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:15753"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57567"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="6.3.4.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="9">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="9 11">Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 9">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9 11">Belongs to the adenylosuccinate synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.4" evidence="9 11"/>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CHB8"/>
    <dbReference type="OrthoDB" id="10265645at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004019">
        <property type="term" value="F:adenylosuccinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0000287">
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044208">
        <property type="term" value="P:'de novo' AMP biosynthetic process"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046040">
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        <property type="project" value="TreeGrafter"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11846:SF0">
        <property type="entry name" value="ADENYLOSUCCINATE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00709">
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        <property type="match status" value="1"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 9 11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 9 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 9 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 9 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 9 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 9 11" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="39"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="9">
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            <position position="67"/>
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    </feature>
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        <location>
            <begin position="38"/>
            <end position="44"/>
        </location>
        <ligand>
            <name>GTP</name>
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    </feature>
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        <location>
            <begin position="39"/>
            <end position="42"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
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        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
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            <begin position="64"/>
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        <ligand>
            <name>IMP</name>
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        <location>
            <begin position="66"/>
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        <ligand>
            <name>IMP</name>
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            <note>ligand shared between dimeric partners</note>
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            <name>IMP</name>
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        </source>
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        </source>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="450" mass="49847" checksum="34AED9D32FA39542" modified="2023-09-13" version="1">MDTNSNQSAVDELNNCITKRRKMETVNKATVVLGAQWGDEGKGKIVDLLATDADVVCRCAGGNNAGHTVVVGEIEYDFHLLPSGIIHKNCKSVIGNGVVVHIPGLFEEAEKAKSKGLHDWKSRLLISDRAHLVFDMHQAVDGLQEMEKGKNCIGTTKKGIGPTYSSKASRSGIRVCDLVGDFEHFSNRFRSLVNVMQRTFPSLEVDVEKQLQMYKNYAEEIRPMVRDTIHYINKTLNETDKLVLVEGANATMLDIDFGTYPYVTSSNCSIGGVCTGLGVPPHKIGNVYGVVKAYTTRVGDGAFPTEQLNGTGETLQVKGQEFGVTTKRKRRCGWLDVVLLKYTQMINGFTGLAVTKLDILDDFEEIKIGVAYRHGGKLLESFPASQNILKEVEVEYETLPGWKSDISGVRKFKDLPENAQKYVRRIETLVGVPIRWVGVGKNRESIIEIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CHX4</accession>
    <name>A0A9Q1CHX4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Gamma-aminobutyric acid type B receptor subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HOLleu_07667</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Nanhai2018</strain>
            <tissue evidence="17">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CHX4"/>
    <dbReference type="OrthoDB" id="2150267at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038039">
        <property type="term" value="C:G protein-coupled receptor heterodimeric complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004965">
        <property type="term" value="F:G protein-coupled GABA receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007214">
        <property type="term" value="P:gamma-aminobutyric acid signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15047">
        <property type="entry name" value="7tmC_GABA-B-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06366">
        <property type="entry name" value="PBP1_GABAb_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000063">
        <property type="entry name" value="Gamma-aminobutyric acid type B receptor subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002455">
        <property type="entry name" value="GPCR3_GABA-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10519:SF20">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 156-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10519">
        <property type="entry name" value="GABA-B RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01176">
        <property type="entry name" value="GABABRECEPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 17" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="713"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="756"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="912"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
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            <begin position="948"/>
            <end position="970"/>
        </location>
    </feature>
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        <location>
            <begin position="976"/>
            <end position="998"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="15">
        <location>
            <begin position="521"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="16">
        <location>
            <begin position="896"/>
            <end position="1020"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="338"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="382"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="1023"/>
            <end position="1050"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="388"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00073785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ8044809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1104" mass="124019" checksum="B22B6CE8AC39845B" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CII3</accession>
    <name>A0A9Q1CII3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HOLleu_09037</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Nanhai2018</strain>
            <tissue evidence="17">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Isomerization of 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>(3E,5Z)-octadienoyl-CoA = (2E,4E)-octadienoyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:45244"/>
            <dbReference type="ChEBI" id="CHEBI:62243"/>
            <dbReference type="ChEBI" id="CHEBI:85108"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(3E,5Z,8Z,11Z,14Z)-eicosapentaenoyl-CoA = (2E,4E,8Z,11Z,14Z)-eicosapentaenoyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:45224"/>
            <dbReference type="ChEBI" id="CHEBI:85090"/>
            <dbReference type="ChEBI" id="CHEBI:85091"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; fatty acid beta-oxidation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CII3"/>
    <dbReference type="OrthoDB" id="14970at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051750">
        <property type="term" value="F:delta(3,5)-delta(2,4)-dienoyl-CoA isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.12.10:FF:000004">
        <property type="entry name" value="Delta3,5-delta2,4-dienoyl-CoA isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000024">
        <property type="entry name" value="Delta3,5-delta2,4-dienoyl-CoA isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.10">
        <property type="entry name" value="Lyase 2-enoyl-coa Hydratase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045002">
        <property type="entry name" value="Ech1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014748">
        <property type="entry name" value="Enoyl-CoA_hydra_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43149:SF1">
        <property type="entry name" value="DELTA(3,5)-DELTA(2,4)-DIENOYL-COA ISOMERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43149">
        <property type="entry name" value="ENOYL-COA HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="8 17" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <keyword evidence="13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="16">
        <location>
            <begin position="274"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="226"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="8"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00051408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00052809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00055786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00071021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ8045918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="673" mass="75519" checksum="2FDE1903304ACE5A" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CIK9</accession>
    <name>A0A9Q1CIK9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cholesterol 24-hydroxylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_09126</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CIK9"/>
    <dbReference type="OrthoDB" id="1470350at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033781">
        <property type="term" value="F:cholesterol 24-hydroxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006707">
        <property type="term" value="P:cholesterol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039983">
        <property type="entry name" value="CYP46A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24293:SF0">
        <property type="entry name" value="CYP46A1 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24293">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 46 SUBFAMILY A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0349">Heme</keyword>
    <keyword evidence="2 3" id="KW-0408">Iron</keyword>
    <keyword evidence="2 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="2">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8045986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="217" mass="25079" checksum="61210458D502DD4E" modified="2023-09-13" version="1">MGEDLPNNILGNIMKYSNLSEDQFDMEIMVDEFLTFFVAAGIPITLKSLFIIPRVRDEIDAVVGDKDFIEYEDVMKMEYTLLVLKETLRLYSPAVGTVRRLAYDMTVGGHHIPAGSSVQMSHYIMARMEKFFKNPLTFDPERFLRDEDKPLYAYFPFSLGTRSCIGQQFALIEARVILAKLYQNYDFKLDPTQSFGILDQLTLKPKGQCMNYIYSRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CKZ2</accession>
    <name>A0A9Q1CKZ2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tyr recombinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_06085</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CKZ2"/>
    <dbReference type="OrthoDB" id="8954815at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="entry name" value="Intergrase catalytic core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35617:SF3">
        <property type="entry name" value="CORE-BINDING (CB) DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35617">
        <property type="entry name" value="PHAGE_INTEGRASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8047163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="204" mass="22945" checksum="C4F76A7F47468126" modified="2023-09-13" version="1">MTHKTLFLLAAASARRRSELHALTTKRGFIRFSHNGVYLIPDPTFLTKNETASFSPGEIYLPNISSSSSIREDRKICPVQALRWYIEKTKNIRSSERLFLIPRSPYNPASKDTLSRWIVNLITPHIDNDDPVHAHQLRAHATSKAWFNGVSLSDILKAAAWKTPSTFVSCYLTDVASNEGAFARSVLGISDQHRRPVLPPSCRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CMW1</accession>
    <name>A0A9Q1CMW1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Steroid 21-hydroxylase</fullName>
            <ecNumber evidence="14">1.14.14.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">21-OHase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Cytochrome P-450c21</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Cytochrome P450 21</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Cytochrome P450 XXI</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Cytochrome P450-C21</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">HOLleu_06714</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="24">Nanhai2018</strain>
            <tissue evidence="24">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="21">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 22">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.14.16" evidence="14"/>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CMW1"/>
    <dbReference type="OrthoDB" id="1844152at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004509">
        <property type="term" value="F:steroid 21-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008202">
        <property type="term" value="P:steroid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006805">
        <property type="term" value="P:xenobiotic metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000049">
        <property type="entry name" value="steroid 21-hydroxylase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050182">
        <property type="entry name" value="Cytochrome_P450_fam2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300:SF397">
        <property type="entry name" value="CYTOCHROME P450 2U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300">
        <property type="entry name" value="CYTOCHROME P450 508A4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 21 22" id="KW-0349">Heme</keyword>
    <keyword evidence="10 21 22" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="13 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 21 22" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0492">Microsome</keyword>
    <keyword evidence="11 22" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="9 22" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="23" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="21">
        <location>
            <position position="449"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00044040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00044116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00044217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00044265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00044282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00044304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00044342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="KAJ8047663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="504" mass="57514" checksum="EC5E6DB57AACACE5" modified="2023-09-13" version="1">MAHVGEKDNSLVNSGVWWDGFPATALLFFSVFIGLMWWTRRHKYLPPGPFFPLRLLSNTNNRKRYLIFIKYGQEYGPIYSLRFGNSLTVVLNNISLAKEALVEKAEVFSERFVTKAVQIGSRGLKGSILMGNGDDYKELRRFGAYALRSFGVGKRSLGSKINEETSHLVEAIHKEENKPFDPVHVISNAVSNIICSISFGDRFDYDNVKFQQLLEVLRTLTTDAPLLNFPVLICPYLMYFPPFSKQIHRNEVVKNFINDVVEKHHATLDANDVRDIIDMFLIEADQRKGETGPDNVYDSSSLWRTIFDLFGAGSDTTANSILWAIAIFCHYPDTQEKVYEEIKAVLGTEKLPVYEDRHRLPYTQALLLEVQRFRPVAPIVPHLCNRDATLGGYHIPKGTRVLINLWSIHHDPEEWKDPELFLPERFLDSDQKVISPDSLVPFSLGRRVCLGETLAKMELFLFLTSLVQRFRFYVPDGMELPSLDGVSGITLSPEPYEVCAEPRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CPC9</accession>
    <name>A0A9Q1CPC9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_01448</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CPC9"/>
    <dbReference type="OrthoDB" id="10064127at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="7">
        <location>
            <begin position="68"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8048933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="397" mass="44832" checksum="4E921AD478D7E363" modified="2023-09-13" version="1">MELTQKDLEDIRKIATPVYPPAPTVNKMRTAKTKVSSSNQKAQGSSTQKCWRCGGKHPPQSCPFKEQKCFKCQKLGHTKSQCDAVEAFKQRKQKQVSQTNQLVEAQPLEIMPEISDLSDDLNHLTGNEQAQVNRLGKNEPFHVSVEVENKPVEFEIDTGSPWSIVPRQIFQEIKHRCKLKESQAELTTYAGNPVHILGEADVSVLACRKKANLTLLVVEEETPLLGRDWIKSLGIKVEIPSSSHDNGQIVKTLNKNLFFQDKLESLLRKHQKLFDSSKLGKLENFQAKVYPTKDEPLFYKATPVSYAARHKIDGALKELESQGIIEPVAFSDYACPLVIVNKHNGKVRLCGNYKLTADKVLKFEQCPIPTLEDLMQDLQGGQYFSKLDLSHAYYQIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CPI5</accession>
    <name>A0A9Q1CPI5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_00363</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CPI5"/>
    <dbReference type="OrthoDB" id="419189at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33332">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8048169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="204" mass="23230" checksum="0E7EA0C50A9AD37B" modified="2023-09-13" version="1">MYADDIQLYITMKSQDKEFTVKKIEDCIHDTKGWMVANFLILNDSKTEVLHVTSKFSNAPQITHLTAGVSQVLSTQSARNLGASFDTNLNMTSHINNISRGASCALRKISKIRKYIDKPTTEKVFHASSASRLVSLTGIKEHITPVIRYLHWLPVNQRITYKILILTFEILNHMSPDYLTELITPYTPSRVLRSSLRSLTNPFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CPZ6</accession>
    <name>A0A9Q1CPZ6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_01088</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CPZ6"/>
    <dbReference type="OrthoDB" id="5814287at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31751:SF42">
        <property type="entry name" value="PROTEIN CBG10204"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31751">
        <property type="entry name" value="SI:CH211-108C17.2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8048680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="254" mass="28722" checksum="E97582769F3E9198" modified="2023-09-13" version="1">MNPATEEIINTELVQVSDTTSSVAMEKHGFRICLDKVEDSGVTVELIATDRHAGIRKLLSEEDARLKLWLKQGSPSHLALEEVVLNKTILKDLDQLVYSCHTGALEVFHNSLLKYCPKRLEFTNPHMEARMRLAVLDHNMNLNRKQAVVKKASRLSRAVGEPRYTLSYSKARKQWVAKKVYEAKTYEYVDQMMVDTLRVFEGNLVTTDVAQPERPRNIANVPRLDKQTVVTHLKARFALPASSSASTDDTGEDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CRZ5</accession>
    <name>A0A9Q1CRZ5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Molybdate-anion transporter</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Major facilitator superfamily domain-containing protein 5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Molybdate transporter 2 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_02157</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Mediates high-affinity intracellular uptake of the rare oligo-element molybdenum.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CRZ5"/>
    <dbReference type="OrthoDB" id="263957at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015098">
        <property type="term" value="F:molybdate ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:monoatomic ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17487">
        <property type="entry name" value="MFS_MFSD5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008509">
        <property type="entry name" value="MOT2/MFSD5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23516:SF1">
        <property type="entry name" value="MOLYBDATE-ANION TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23516">
        <property type="entry name" value="SAM (S-ADENOSYL METHIONINE) TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05631">
        <property type="entry name" value="MFS_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="46"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="76"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="107"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="131"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="174"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="204"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="250"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="283"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="310"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="344"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="375"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="407"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00032555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8049414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="444" mass="49182" checksum="5790AC361C4BBD92" modified="2023-09-13" version="1">MAVREFVYLALTALAVVYSLMHVICRVNRSKNINNPTFVNFQKKYFLAYFLALLADWLQGPYLYKLYSHYGFLEPQIAVLYVCGFAASVVFGVFTHTLADRYGRKKLCITFCIVYSVSCFTKLSTSYTVLIFGRILGGISTSLLFTAFEAWYVHEHVETHDFPKEWLSLTFSTASFWNGVLAISAGIVANMFAGVFALGPVSPFILAIPFLFASGAIVAISWGENYGENKRSFSKTCSEGAKNIGGSRRIVLIGAMQSLFESVMYIFIFIWTPVLDPHHPPLGLVFSSFMICIMIGTTFYNYIASFTQAASLFVNGSIVLALISTVICIGSTKPGDEHPTISYVAFLLLEFACGMYFPAMGFLRGRILPEAHTVGIMNWFRLPLNLIACIGLMVLHGDPSTSGTRHMFIICSCLLGLALFCGIRFAALTKDDENLKNPSTEEAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CSZ2</accession>
    <name>A0A9Q1CSZ2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_03746</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CSZ2"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8050515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="50" mass="5732" checksum="6C7171C3B32FD9FA" modified="2023-09-13" version="1">MAPTAFVLGAQHKQWSRANKPVSHNWKKGRGRLDLNQLVGNWDVRPHLTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CTJ3</accession>
    <name>A0A9Q1CTJ3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_04633</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8051168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CTJ3"/>
    <dbReference type="OrthoDB" id="8300196at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47018">
        <property type="entry name" value="CXC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8051168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="216" mass="24221" checksum="3B2EBB5D50BB2ED1" modified="2023-09-13" version="1">MMGGLHVEKALLKVIGDFLEGSGWTSVMTSAGVTTEGRAESLQKGTQTSRSQWAQLMFAMDHYHYARWLTLHVTDLQELPNDSVDIHRAFVKGNFVTQKSSHKFSALAHDEIHEQQNAIVKGNGRVIGITENEAALRRWMVAGSELAGIVSEFEDQFQRQKQTDVRYHEQLPSVQKSFASDLNNAISSFENWGIRSQKTVMTCMCLTPRSSCLMKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1GYM2</accession>
    <name>A0A9Q1GYM2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein eL31</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">60S ribosomal protein L31</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_29788</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8027748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1GYM2"/>
    <dbReference type="OrthoDB" id="9739313at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00463">
        <property type="entry name" value="Ribosomal_L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.440.10:FF:000001">
        <property type="entry name" value="60S ribosomal protein L31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000054">
        <property type="entry name" value="Ribosomal_eL31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020052">
        <property type="entry name" value="Ribosomal_eL31_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023621">
        <property type="entry name" value="Ribosomal_eL31_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002258">
        <property type="entry name" value="PRK01192.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10956">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10956:SF0">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01198">
        <property type="entry name" value="Ribosomal_L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01380">
        <property type="entry name" value="Ribosomal_L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54575">
        <property type="entry name" value="Ribosomal protein L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01144">
        <property type="entry name" value="RIBOSOMAL_L31E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8027748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="122" mass="14314" checksum="DA62E747D5FE424D" modified="2023-09-13" version="1">MAKKDKKKGRSALNDVVTREYTIHLHKRVFGMSFKKRAPRAIKEIRKFAQKTMGTEDVRLDTRLNKHIWAKGIRSVPHRVRVRLARKRNEDEDSPHKLYTLVTYVEVPSFKGLQTLNVDSDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H0L4</accession>
    <name>A0A9Q1H0L4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="22">Glutamate receptor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">HOLleu_28617</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="22">Nanhai2018</strain>
            <tissue evidence="22">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Postsynaptic cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H0L4"/>
    <dbReference type="OrthoDB" id="5984008at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015276">
        <property type="term" value="F:ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019594">
        <property type="entry name" value="Glu/Gly-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001508">
        <property type="entry name" value="Iono_Glu_rcpt_met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015683">
        <property type="entry name" value="Ionotropic_Glu_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001320">
        <property type="entry name" value="Iontro_rcpt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18966">
        <property type="entry name" value="IONOTROPIC GLUTAMATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00060">
        <property type="entry name" value="Lig_chan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10613">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00177">
        <property type="entry name" value="NMDARECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00918">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00079">
        <property type="entry name" value="PBPe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="8 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="9 22" id="KW-0675">Receptor</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="19" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0770">Synapse</keyword>
    <keyword evidence="4 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040255348" evidence="19">
        <location>
            <begin position="22"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="568"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="635"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="833"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor C-terminal" evidence="20">
        <location>
            <begin position="442"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor L-glutamate and glycine-binding" evidence="21">
        <location>
            <begin position="442"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="522"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="527"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="694"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="744"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="site" description="Crucial to convey clamshell closure to channel opening" evidence="16">
        <location>
            <position position="672"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with the cone snail toxin Con-ikot-ikot" evidence="16">
        <location>
            <position position="699"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="757"/>
            <end position="812"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00034100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM00079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="SMART" id="SM00918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KAJ8029264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="902" mass="103925" checksum="EE65579A5F79C483" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H1C9</accession>
    <name>A0A9Q1H1C9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_28287</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H1C9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8029010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="120" mass="13290" checksum="E338CEA8B7B03B4E" modified="2023-09-13" version="1">MGQTLKTLFPRYLKGSKENSLLLLFCRCRCGDSSLSGNTVGISYNPQPTGAMMGIYCTIKISYIADEVGCTPGLKHSCKDEDKQPCVALIVVGSEPGSLRLVFGGRQLLKEWKYKKNWHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H2G3</accession>
    <name>A0A9Q1H2G3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Adenosine receptor A2a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_27057</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H2G3"/>
    <dbReference type="OrthoDB" id="10042731at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00637">
        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050569">
        <property type="entry name" value="TAAR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24249:SF422">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24249">
        <property type="entry name" value="HISTAMINE RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="50"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="90"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="128"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="171"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="217"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="267"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="70"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8030598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="377" mass="42117" checksum="9065CE149C3403CA" modified="2023-09-13" version="1">MNTAVDALLTQFPSMKVTATYGDYGTTDTSTDGDVDIVSDLPPLHLSVSVYWLVFYIFVFGTIFLGSIIGNQLVIWSYIIEPKLRRPTNILIVSLAVADFITGLIGLPTAIFGRLFRGPYVCFAATRPFLHIASFMMCSVSVDHLVLITIDRYIAVTRPLKYPTIMTTRKCRIMIAIAWLNASFFGAIPVLTRPPNPTLWVCGRTKYEGSSFRIHRLASVAFIPVILVFLCLVYIRLYIVAMKHVREQSERIKAQTKKDDGSSARSAMIRTTVTTAIVLGAFAICWLPITAKFFVEVFVYTTEEALFTIKTAADVLSLGNSMVNPIIYGYRNALFRSTYKRILRSVCCCGFKDHEKERPNLQTQSNSTSVATISTTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H3T9</accession>
    <name>A0A9Q1H3T9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_25899</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H3T9"/>
    <dbReference type="OrthoDB" id="10261408at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="5"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8032384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="156" mass="17308" checksum="F79B46A1726D95A8" modified="2023-09-13" version="1">MSHVCHICHLPFTRGFNLRRHIERFHKQNHGDLEMRGGNSKKGIAKRPHDSSDEGSYGNNSESDTNDGASEAKEEDAMTESSETDDSDPDSDTEEADSSEEESSEEDILTDKDGEKDDAPGNVCCPIDNWKALVQMNKAMGIILKREFKKTTDSAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H3X4</accession>
    <name>A0A9Q1H3X4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Proteasome subunit alpha type</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_24556</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="5">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the peptidase T1A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H3X4"/>
    <dbReference type="OrthoDB" id="431557at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03749">
        <property type="entry name" value="proteasome_alpha_type_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000063">
        <property type="entry name" value="Proteasome subunit alpha type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035144">
        <property type="entry name" value="Proteasome_alpha1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00388">
        <property type="entry name" value="PROTEASOME_ALPHA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2 4 5" id="KW-0647">Proteasome</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome alpha-type subunits" evidence="6">
        <location>
            <begin position="17"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8031381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="266" mass="29662" checksum="5014CAA5E684C927" modified="2023-09-13" version="1">MTDTKYRWTQVKFRNQYDNDVSVWSPQGRIHQIEYAMEAVKQGSATVGLKSKTHAVLVALKRASSELSAHQKKIILIDDHVGISIAGLTSDARLLSKFMRTECLNSRYAYDMPLPVSRLVSAVGNKSQLQTQRYGGRPFGVGLLVAGYDDMGPHIFQTCPSANYFDCKCMAIGARSQSARTYLEKKMSEFPDCTLEALVMHGLRALRETLPSETELNLKNCSVAVVGKDQNFQIYEQDEVKTFMGTSAIDDDNSTKTLLIDDDAEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H451</accession>
    <name>A0A9Q1H451_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Neuronal acetylcholine receptor subunit alpha-9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_25094</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H451"/>
    <dbReference type="OrthoDB" id="6142676at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005230">
        <property type="term" value="F:extracellular ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18989">
        <property type="entry name" value="LGIC_ECD_cation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19051">
        <property type="entry name" value="LGIC_TM_cation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000028">
        <property type="entry name" value="AcetylCholine Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040395103" evidence="7">
        <location>
            <begin position="27"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="260"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="293"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="325"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="474"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="8">
        <location>
            <begin position="48"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="9">
        <location>
            <begin position="265"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="383"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8031785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="507" mass="58856" checksum="B53E9940A2683C2A" modified="2023-09-13" version="1">MADKRVSGLGWRVYPIFIIFLLGASAKYDKECDEEPRDDYDEIKTPEARLLERLLDTYGPTSPRPILDVNETMYVGLEVKPISLISFEEQSQLITLKSDMLVTWVDEQLVWDPADHDGVETVDISVEEIWTPDLTVPESTGESFEVHNNMIATVDYMGNVAWYSVAVTETFCKLLVRYFPFDVQHCNITYISWLYERKRLEINFSPFDSYEKVIETFVKNGMWRLSEINIDDSILTYCGGCDEYSYVRFTFEFERTEASFFALNVILPCILLSMMNLLVFFLPPESGEKVSFVMTNVLTLVLFQQLVATMLPASGDDTPLLSTHIVTLIFFGCIAIVWTVFVTNLFHKTTPYPKWLYSIVTSFRFLYRRTQFGKEEAKARKTLRESRDALDGDDNENELNDIQRDDDGTEQPSGHRWYHYQNAKRKIQTFSCFKCGKDNATIVEGKRRRKKPRINFDPQDVRLWQETASLLDKFMGYVMIFVTVIIYIVLLSSFASHDPHGYQLDKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H4V4</accession>
    <name>A0A9Q1H4V4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ficolin-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_23031</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8032938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H4V4"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00087">
        <property type="entry name" value="FReD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19941">
        <property type="entry name" value="TIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050373">
        <property type="entry name" value="Fibrinogen_C-term_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036084">
        <property type="entry name" value="Ser_inhib-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002919">
        <property type="entry name" value="TIL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025615">
        <property type="entry name" value="TILa_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040941">
        <property type="entry name" value="GGGWT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143">
        <property type="entry name" value="FIBRINOGEN/TENASCIN/ANGIOPOEITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01826">
        <property type="entry name" value="TIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12714">
        <property type="entry name" value="TILa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00186">
        <property type="entry name" value="FBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57567">
        <property type="entry name" value="Serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="111"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="3">
        <location>
            <begin position="147"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8032938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="383" mass="43286" checksum="592E60FD7DCDFCCE" modified="2023-09-13" version="1">MMIISTICYQQAPKVTSAFISADDLIVMCSSNMVYGACVCQATCSNPGDCYKNCVDGEETCFCPDGHFLNETNNCVLQQQCGCFVDGYGIVPNGGKYVNSNCTRECFCNDNQLTCDEHFQCSTNATCQQLDDIHECRCKDAFEGDGQTCIALPTDCMELYNAGNTADGVYTIYPKSWSGSKFDVYCDMKTDGGGWTIFQRRVNGLVNFYQNWASYKRGFGEKTGEHCLGNDKIHSLTHSKNYQLRVDIVDSGGRPYHALYDLFRIENESHKYKLVNLGSFSGTAGYNSMASNHNRNFSTYDQDNSASRYNAAEKHGGAWWYHYTRRSDTPHCHTWSEDDTYWYCSNSNLNGDYSVERTDNIFWFLLPGNDCAIHQTEMKIRPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H6D7</accession>
    <name>A0A9Q1H6D7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Protein NLRC5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_21299</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="427518at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.220.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000906">
        <property type="entry name" value="ZU5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46844">
        <property type="entry name" value="SLR5058 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46844:SF1">
        <property type="entry name" value="SLR5058 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00791">
        <property type="entry name" value="ZU5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51145">
        <property type="entry name" value="ZU5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="93"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="NACHT" evidence="3">
        <location>
            <begin position="451"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="domain" description="ZU5" evidence="4">
        <location>
            <begin position="1188"/>
            <end position="1327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="742"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="833"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="742"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="767"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="871"/>
            <end position="884"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8034453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1476" mass="167656" checksum="5C5ED8835E101FD0" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H748</accession>
    <name>A0A9Q1H748_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Centrosomal protein 20</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">FGFR1OP N-terminal-like protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">LisH domain-containing protein FOPNL</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HOLleu_19076</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Nanhai2018</strain>
            <tissue evidence="15">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in the biogenesis of cilia. Required for the recruitment of PLK1 to centrosomes and S phase progression.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium basal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Microtubule organizing center</location>
            <location evidence="3">Centrosome</location>
            <location evidence="3">Centriolar satellite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasmic granule</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CEP43 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8038097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H748"/>
    <dbReference type="OrthoDB" id="5970631at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034451">
        <property type="term" value="C:centriolar satellite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034453">
        <property type="term" value="P:microtubule anchoring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.960.40:FF:000002">
        <property type="entry name" value="LisH domain-containing protein FOPNL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.960.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018993">
        <property type="entry name" value="FOP_dimerisation-dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15431:SF19">
        <property type="entry name" value="CENTROSOMAL PROTEIN 20-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15431">
        <property type="entry name" value="FGFR1 ONCOGENE PARTNER/LISH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09398">
        <property type="entry name" value="FOP_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00667">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FGFR1 oncogene partner (FOP) N-terminal dimerisation" evidence="14">
        <location>
            <begin position="62"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="166"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="166"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00055043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00070736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00076755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00081996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF09398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ8038097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="186" mass="21858" checksum="0584F9442DA8099B" modified="2023-09-13" version="1">MYVTLPVQSICLYIRRMDYTNSLGIYSVLKETLENRGVLGQIKARMRAEVFHALDDQLESRPPLSNENMLINELIREYLEFNNYKYSLSVLMAETGQPQTPLDRSFLTNELNITEDRGTTKLPLLYGVLSHFLESKRLGFSFHQRKEHDGRTNSQKHRQRDYLDEKDLMEGMHEEEPVLFSGLKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H7X6</accession>
    <name>A0A9Q1H7X6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_20327</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H7X6"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8036374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="120" mass="13029" checksum="031FA041EC32F6CE" modified="2023-09-13" version="1">MAKIVTDAAGVAAETPGVVRDVSNWAQELQTLTSGNELSSSTELLVNLLGRIYEAFLKNKHLQEEIVKALDSKEGAMLLCGSSPFCQRPRILLLRHKNAYTSVSIYYGTSAISVATLCKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H8I4</accession>
    <name>A0A9Q1H8I4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_20176</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H8I4"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8036256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="106" mass="12411" checksum="91E12E27B191EF87" modified="2023-09-13" version="1">MFYTSVCAKLTGKCLYRNLRIKWKMPLKRFRIQLYLNLTNCIFLPILLGGTSRIFRWERGATEWSARVKCIKMQQTHGTTCENSVAACFLRIELMYTAEVRGIKLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HAF3</accession>
    <name>A0A9Q1HAF3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_19140</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8038146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HAF3"/>
    <dbReference type="OrthoDB" id="1022360at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="57"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8038146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="79" mass="8943" checksum="7A77D51E5E2743F4" modified="2023-09-13" version="1">MICNINEFAPEALYRNEYTKASDVWSMAVVIWEMLSGEKENNSFKLFAVKSPRKVAPCKSPPSQQSPKISPLKSRSSTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HCF0</accession>
    <name>A0A9Q1HCF0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_14374</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HCF0"/>
    <dbReference type="OrthoDB" id="5667at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008028">
        <property type="term" value="F:monocarboxylic acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF303">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040361671" evidence="2">
        <location>
            <begin position="23"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="128"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="156"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="180"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8040156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="243" mass="26427" checksum="D536AF67CAFD0ECC" modified="2023-09-13" version="1">MLIFSSAYCTLLLLGLITPSQNNCDGEDEQSPSEIPQAICNKKSKSNDAQEHEELLPVLEGKVSSSKSEERVYASKDKANRFISLIISHPYVLVLVLLSSSYDYVLSSWSIFLVPLGESLGLTSETAVWLSTVSGLSGFVGRLFCIAMYFWSDINLQTGFTIPYLLIACGCLLTVFFKRFLWLAVAASLGGFGVAVLGNMTSTVIPACVCDKHFQVTVILLYTASGIMTQFGGIATGKLYNLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HCI6</accession>
    <name>A0A9Q1HCI6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_07047</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HCI6"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8044322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="93" mass="10883" checksum="90E80BA37E682E86" modified="2023-09-13" version="1">MDIGVVQMCTSFNVAHSTVVWYFALQMKFTSLKNVTQPSSCTGSFQIKTALQYFDLRRVLQNNKLMFFCFVIKYMVFFFIGNIFSVSYYVSYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HCS9</accession>
    <name>A0A9Q1HCS9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Eukaryotic translation initiation factor 2 subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Eukaryotic translation initiation factor 2 subunit beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_12169</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eIF-2-beta/eIF-5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HCS9"/>
    <dbReference type="OrthoDB" id="10255414at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005850">
        <property type="term" value="C:eukaryotic translation initiation factor 2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.170:FF:000001">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045196">
        <property type="entry name" value="IF2/IF5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002735">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016189">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016190">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_Zn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23001">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23001:SF3">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01873">
        <property type="entry name" value="eIF-5_eIF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00653">
        <property type="entry name" value="eIF2B_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100966">
        <property type="entry name" value="Translation initiation factor 2 beta, aIF2beta, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75689">
        <property type="entry name" value="Zinc-binding domain of translation initiation factor 2 beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Translation initiation factor IF2/IF5" evidence="7">
        <location>
            <begin position="210"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="52"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="79"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00040874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00042452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8041369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="344" mass="38842" checksum="0666C19192ADD69E" modified="2023-09-13" version="1">MAEDEAIFDPTMKKKKKKKKTPFEFEATQEGGDAAAPEPVQNLEDGDVGKPAVEEKKEEETSVKADLDTDDLDLDNFSKMKKKKKKKARFDMDSLAETLPDGDAPNEESTQDANDGSSAPPPSDDVDLDLEGDAELFTKNMKKKKKKKRVTIEDSLENGQVENQKDEDVDAQIKAGIPWAGTERDYTYEELLTRVFDIMKAKNPDMVGDKKRFVMKPPQVVRVGTKKSSFVNFSDICRMLHRQPEHLLAFLLAELGTSGSIDGQNMLIIKGRFQQKQIENVLRRYIKEYVTCHTCRSSETILHKENRLFFLQCESCQSRCSVASIKSGFQAVTSKRAQMRAKAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HDF0</accession>
    <name>A0A9Q1HDF0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">MADF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_13071</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8042089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HDF0"/>
    <dbReference type="OrthoDB" id="5803771at2759"/>
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        <property type="component" value="Chromosome 5"/>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:transcription regulator complex"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="BESS_motif"/>
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        <property type="entry name" value="MADF-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039353">
        <property type="entry name" value="TF_Adf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12243">
        <property type="entry name" value="MADF DOMAIN TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12243:SF60">
        <property type="entry name" value="SI:CH211-15D5.12-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02944">
        <property type="entry name" value="BESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10545">
        <property type="entry name" value="MADF_DNA_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MADF"/>
        <property type="match status" value="1"/>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="43"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="BESS" evidence="4">
        <location>
            <begin position="235"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="165"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="216"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="167"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8042089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="280" mass="32619" checksum="F3A7C77CC1230074" modified="2023-09-13" version="1">MYIHISVPSMRRVQLWHIRDVLVTMSSQRELPPRKMECSADGKLIFLVSKWKELYDKNHERFKDTEWKDGIWREICEELPIVVDDVELVKKRWKNLRDTYLKRKKESLKNGTENRNPLREWKHLNSMGFLDPYLRYLYYVPKDTGVYSVSVGEDAVEVYCEMEEEQASTSDGVNTKESLPLSTGTSVSTPHTSRNETPVIPTNSILCMKRKASDTFSEETPKHLNHKMSPKQPKETPDELFVLSLVPALERMSHQQNALARMRIQQVLYDIEFGQGITNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HDG0</accession>
    <name>A0A9Q1HDG0_HOLLE</name>
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        <submittedName>
            <fullName evidence="7">NACHT domain- and WD repeat-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_13108</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8042119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6134417at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
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        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09275">
        <property type="entry name" value="RNase_HI_RT_DIRS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03714">
        <property type="entry name" value="RT_DIRS1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="entry name" value="Intergrase catalytic core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="AAA+_ATPase"/>
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        <property type="entry name" value="DNA/RNA_pol_sf"/>
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        <property type="entry name" value="DNA_brk_join_enz"/>
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        <property type="entry name" value="Integrase-like_cat_sf"/>
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        <property type="entry name" value="NPHP3-like_N"/>
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        <property type="entry name" value="NWD1/2-like_WH"/>
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    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
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        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
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        <property type="entry name" value="BETA TRANSDUCIN-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19871:SF45">
        <property type="entry name" value="NACHT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24883">
        <property type="entry name" value="NPHP3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25469">
        <property type="entry name" value="WHD_NWD1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
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        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47823">
        <property type="entry name" value="lambda integrase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="6">
        <location>
            <begin position="1150"/>
            <end position="1332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="9"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="483"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="519"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="620"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1013"/>
            <end position="1047"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1645"/>
            <end position="1672"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="2007"/>
            <end position="2031"/>
        </location>
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        <location>
            <begin position="28"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="50"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="483"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="627"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="674"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="684"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="704"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="716"/>
            <end position="725"/>
        </location>
    </feature>
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        <location>
            <begin position="729"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="755"/>
            <end position="773"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8042119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="2967" mass="330019" checksum="43D70EB39B269912" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HDL9</accession>
    <name>A0A9Q1HDL9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Cytochrome P450 2C7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_13303</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8042284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HDL9"/>
    <dbReference type="OrthoDB" id="1055148at2759"/>
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        <property type="component" value="Chromosome 5"/>
    </dbReference>
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        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000238">
        <property type="entry name" value="Cytochrome P450 2A6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050182">
        <property type="entry name" value="Cytochrome_P450_fam2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300">
        <property type="entry name" value="CYTOCHROME P450 508A4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300:SF417">
        <property type="entry name" value="CYTOCHROME P450 508B1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0349">Heme</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0492">Microsome</keyword>
    <keyword evidence="11" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8042284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="429" mass="48592" checksum="29A47DCEA096885E" modified="2023-09-13" version="1">MSMKSVRLKIAMVYSQDDSSRGLPKGPPGLPLLGNLLLFRSRAYPVDEIMTNWAKEYGNIYRLKIGPKSMYILNGYDCINEVFSNPDVDGRNTAYTFKAGLGEGAGTASSEGQAWSCQRHFTLKYFSSSTERLEKAISEEVTRILSALNSKPQTPIDITNDVFRLAFYSIVCKVAFAKNYNYTDGELKAIADIWDTVQRNRGIVSLNLSLKLPFGKSFKIFQRVCEMKSLLQKYFQERRNYLQSKFATPSCFVDKYLLQQNEVQGKEISKEFTDSNLLWSTFELFYTGTNIPNSTLSSIMVYLINNPHVQEKIQHELDTQLGDKPPSWVDRQRLPYTQAAIAESMRHSNNIFLGVPRVARQPVTIGEYHFPAGSTFMSNLHSVMMDPGAFPSPEEFHPERFLDQDGNFRKPKELVPFGMGACGPAPVYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HGD8</accession>
    <name>A0A9Q1HGD8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Zinc finger C2HC domain-containing protein 1C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_08514</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ZC2HC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HGD8"/>
    <dbReference type="OrthoDB" id="10255185at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049899">
        <property type="entry name" value="Znf_C2HC_C3H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026104">
        <property type="entry name" value="ZNF_C2HC_dom_1C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14649">
        <property type="entry name" value="ZINC FINGER C2HC DOMAIN-CONTAINING PROTEIN 1C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14649:SF1">
        <property type="entry name" value="ZINC FINGER C2HC DOMAIN-CONTAINING PROTEIN 1C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13913">
        <property type="entry name" value="zf-C2HC_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52027">
        <property type="entry name" value="ZF_C2HC_C3H"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2HC/C3H-type" evidence="8">
        <location>
            <begin position="502"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="domain" description="C2HC/C3H-type" evidence="8">
        <location>
            <begin position="610"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="152"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="305"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="365"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="587"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="625"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="157"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="223"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="319"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="399"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="409"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="433"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="631"/>
            <end position="647"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS52027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8045494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="647" mass="75678" checksum="58B426B3C2E8A5D6" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HGP2</accession>
    <name>A0A9Q1HGP2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Reverse transcriptase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_08128</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09274">
        <property type="entry name" value="RNase_HI_RT_Ty3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000115">
        <property type="entry name" value="Polyprotein of retroviral origin, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000032">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 297-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon gypsy-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054465">
        <property type="entry name" value="Integrase_p58-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041373">
        <property type="entry name" value="RT_RNaseH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22938">
        <property type="entry name" value="Integrase_p58_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17917">
        <property type="entry name" value="RT_RNaseH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="10">
        <location>
            <begin position="857"/>
            <end position="1015"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase" evidence="9">
        <location>
            <begin position="1271"/>
            <end position="1448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="325"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="590"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="690"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="78"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="325"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="340"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="364"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="590"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="634"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="659"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="706"/>
            <end position="730"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8045180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1663" mass="190185" checksum="F734D599ACB1A643" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HHW3</accession>
    <name>A0A9Q1HHW3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_08552</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HHW3"/>
    <dbReference type="OrthoDB" id="10011873at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8045526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="176" mass="20146" checksum="9CBE99A88EABA97B" modified="2023-09-13" version="1">MSRGRRVSTFTLDLNYLSSLWPAESKFLVRSGDGPPVSLDKTARKEDFDGIGGFEPCDTVRLNADRGYLLWVNPRGSVQVKFKYGRTGDDFKCFVNPEHKGVKVYEVRSFGRELIIDGKLPFEENPRYMKKSAVRPRENNFEFQSKNGELNLLLEVVQDDGFQLPPAFKLNYVLTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HI10</accession>
    <name>A0A9Q1HI10_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="16">3-beta-hydroxysteroid-Delta(8), Delta(7)-isomerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HOLleu_06387</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Nanhai2018</strain>
            <tissue evidence="16">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EBP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HI10"/>
    <dbReference type="OrthoDB" id="58557at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000247">
        <property type="term" value="F:C-8 sterol isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047750">
        <property type="term" value="F:cholestenol delta-isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004769">
        <property type="term" value="F:steroid Delta-isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006695">
        <property type="term" value="P:cholesterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007905">
        <property type="entry name" value="EBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033118">
        <property type="entry name" value="EXPERA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14207:SF0">
        <property type="entry name" value="3-BETA-HYDROXYSTEROID-DELTA(8),DELTA(7)-ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14207">
        <property type="entry name" value="STEROL ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05241">
        <property type="entry name" value="EBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51751">
        <property type="entry name" value="EXPERA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 13 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="7" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="10" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="4 13 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="30"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="64"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="120"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="162"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="186"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="EXPERA" evidence="15">
        <location>
            <begin position="64"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ8047399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="235" mass="26744" checksum="1638612CB6FB3FE8" modified="2023-09-13" version="1">MADQVKSNVVHPFTPPSLDIPGYVPNTIPILNILAIYFGLVLVVLVGTVALFSKNRNGDKLGIVDRLLMAWFAICGFTHILLEGTYVILGSSVVWRDDIFSQIWKEFGKADSRYMIRHEALISVEAITAFLEGPACFLIMWMTYRRSPGRYALEMLVSTGQLYGTILYFLEAYLMGFAHMPYGHPIYFWIYFVGFNFPWIVIPTSVIIRDYTNFSASQSLRDNSAKSKQVTKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HJE4</accession>
    <name>A0A9Q1HJE4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_00045</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8047925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HJE4"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF13">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="78"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8047925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="227" mass="26940" checksum="EF39DF67B93695D1" modified="2023-09-13" version="1">MPFNLSELVASPSVEELDSLKKSEIVKVAEHYGIEFQPFMRKDKIKRYVLEYLVDESILPSTVLETAITVPTDNTFKLKRLEMEMNKEIRLKEVEREREREEREMQMQKEKEEREMQMQMQREEKAREHEFRLKQLELGVIKGSDPKIGLDTGGFNVSKHVKFVPKFQEDNVEKFFNHFEKLGEQLKWPRDKWSIIIQSNFTGKAQEVYSALSIEDSMDYDKVKKAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HJM4</accession>
    <name>A0A9Q1HJM4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_06547</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HJM4"/>
    <dbReference type="OrthoDB" id="10035172at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="300"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8047521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="364" mass="41010" checksum="D8E9E6FC4C377269" modified="2023-09-13" version="1">MDGRAATTSTEGGDGEFQKKRKLRDAGAVLARKRLANIAKHSAYMRKWTGIINNAEKNKLQNCEKQMLKNQRITDVKITRYKEQLKQELYEMRAEKRALHIHSVLEEKDEAEQRKKIERYAARGRYLYAIRNETKAQKEGMESYSERNETVRKLLAPARKESMTAMTNCELMGSADPANYFKVGRRLSKIDLSNCVYYSSDEDDEDDTELVGAIKMSSLLTQQNMEAELSDNRPITSGSSTPSINLNKLRQELGSDLRKQLDAIFIDDPEYQMLPLTRANTASADSKVAAQLVEEGLMPPVSLEGNENDGISKVVDETSYTQSQEMERKPKLKLPPLKADKSVLPSLVARPTVAQPGLSTVVEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HMB7</accession>
    <name>A0A9Q1HMB7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EamA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_04343</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HMB7"/>
    <dbReference type="OrthoDB" id="306876at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="105"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="9"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8050953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="171" mass="19272" checksum="99B4CC30A7C2C141" modified="2023-09-13" version="1">MSLVLNRNLSKLGVNSRPIFCMFFIGMIVVCISGFVSMILCRWRCPAMRDWLYVLLAGVTYAVSYFSVFYALKVETATRVTVLITSEIVWAFLWQVIFIRMPPFWTSYIVAILILTACIGITLKNKPRPPQSRDSRVFSLRGVEQSDNTITVLTLTYAPNVVCSGVLGPSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HMD7</accession>
    <name>A0A9Q1HMD7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_04590</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8051136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HMD7"/>
    <dbReference type="OrthoDB" id="5986074at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8051136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="166" mass="18127" checksum="81EEB9EA7E6CE4B4" modified="2023-09-13" version="1">MWRARITVGGGAEENPRSSRVRSSSRISTACVRLVSVKFILDIIASALDPPKFSPTVTDIKGAVALFPGKLVTYRSFKSAGPRNMRSTSEAEFLKAVEELTAFGKVVEVRVPRATRKTAVFLRKAPGEIQEALWTTSIGQEAFARQFSKATHSKVSKNIAAKIAEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0Y8A7</accession>
    <name>A0A9Q0Y8A7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase TPTE2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_45152</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010001962">
        <property type="protein sequence ID" value="KAJ8017437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0Y8A7"/>
    <dbReference type="OrthoDB" id="16692at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016314">
        <property type="term" value="F:phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051281">
        <property type="entry name" value="Dual-spec_lipid-protein_phosph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029023">
        <property type="entry name" value="Tensin_phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12305">
        <property type="entry name" value="PHOSPHATASE WITH HOMOLOGY TO TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12305:SF60">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3,4,5-TRISPHOSPHATE 3-PHOSPHATASE TPTE2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51181">
        <property type="entry name" value="PPASE_TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatase tensin-type" evidence="3">
        <location>
            <begin position="76"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8017437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="161" mass="18717" checksum="FBBE1C4AA4BC899C" modified="2023-09-13" version="1">MFFRTPFPDIMDLCIVVITGVVTFTYVIADFSGQNSDYGKLILACRFIRVVMLARLISERRHLTKASRRMVSENKRRYRSDGFDLDLTYITDNIIASSFPSSGSQSFYRNPISEVSRFLNTKHAGHYRVCNLCSERHYDESFFNNRVARFPIDDHNVPLLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YAT2</accession>
    <name>A0A9Q0YAT2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_42080</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000028">
        <property type="protein sequence ID" value="KAJ8019372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YAT2"/>
    <dbReference type="OrthoDB" id="3268967at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00303">
        <property type="entry name" value="retropepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF1">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="315"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="29"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="283"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="351"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="524"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="29"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="60"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="72"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="283"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="353"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="540"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="557"/>
            <end position="568"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8019372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="568" mass="62324" checksum="87001A10C7A42E69" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YAX3</accession>
    <name>A0A9Q0YAX3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Zinc finger MYM-type protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_44429</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000875">
        <property type="protein sequence ID" value="KAJ8017895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YAX3"/>
    <dbReference type="OrthoDB" id="5987958at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45749">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45749:SF21">
        <property type="entry name" value="DUF4371 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8017895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="127" mass="14170" checksum="762D6343FCB8003B" modified="2023-09-13" version="1">MDGASNMSGKERGLSTRIKEVAPKAIYVHCYGHVLNLALQDTMSTLEPLRNALGVIQSLYNLLEASSKRPAFFEKVKAQQEDEHAQKLTLKSLSVTRWSCRYDAVKAVINQLSSIMKALVTLSEERC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YAY8</accession>
    <name>A0A9Q0YAY8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Ectonucleotide pyrophosphatase/phosphodiesterase family member 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_44371</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000828">
        <property type="protein sequence ID" value="KAJ8017925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YAY8"/>
    <dbReference type="OrthoDB" id="415411at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd16018">
        <property type="entry name" value="Enpp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002591">
        <property type="entry name" value="Phosphodiest/P_Trfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10151">
        <property type="entry name" value="ECTONUCLEOTIDE PYROPHOSPHATASE/PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10151:SF126">
        <property type="entry name" value="ECTONUCLEOTIDE PYROPHOSPHATASE_PHOSPHODIESTERASE FAMILY MEMBER 7-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01663">
        <property type="entry name" value="Phosphodiest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8017925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="285" mass="32339" checksum="2A2B56FA0E9C5A44" modified="2023-09-13" version="1">MTDNELNCLILIYTEWIDWPKVILFLIDGLRYDQYGYELPAFYNVEASGVRAEWMDGAFVTLSAPSMYTIATGLHPESHGVVHNLYYDPETGEKTTSYSATLNVSEWWDTGVEPIWVTAKLQGLSVGNIMYPGGMKEVKGIVPDKIIPSDSWWWWNMSLDERVDLAIQWLVEDDFDLVLLYADKPDTLCHTFGTDSPAVKASLDSIDLSVQRLFDQIEENGLSGSVNVIMVSDHGHMNFLRGKQVRLFDYINGSDIEFHMANYGPTFQILPKEGKLEKVRLSACF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YB84</accession>
    <name>A0A9Q0YB84_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Chromatin modification-related protein png2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_43790</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000461">
        <property type="protein sequence ID" value="KAJ8018294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YB84"/>
    <dbReference type="OrthoDB" id="10005682at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd22343">
        <property type="entry name" value="PDDEXK_lambda_exonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011604">
        <property type="entry name" value="PDDEXK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019080">
        <property type="entry name" value="YqaJ_viral_recombinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47526">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47526:SF3">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09588">
        <property type="entry name" value="YqaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="6">
        <location>
            <begin position="50"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="5">
        <location>
            <begin position="450"/>
            <end position="508"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8018294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="508" mass="58012" checksum="70C712A30DB27DCB" modified="2023-09-13" version="1">MKNYKSLEAHHFFRAGWVQTVFHHLTTSNNFIFKAYVKPSQRVTEKPHRPWVAVDKDGIVLSAHCDCMAGLGESCSHVAALLFKIEAAVRFGITSLACTDEPCKWNNFFVKDVECSPISGIQFNSNEALNKHKTSTERSTAKMAASSECEQTNFLFALSSCKTSQPVCLSNYHEFCSPFKDKNTYISDRLPTSFKLLFDANSVKLNEVELSEKCEAFISSLSISDDQINYLESITVNQAQDIVWHEQRIGRITASRVSDIMKTNFSNPSNSLLLNLTSHEVQNLNAPPLVRGRENEPKVFTLYEKEYLSCYHRDGSVTKVGMKLSKEYPWLGASADGLISCSCHGSCTVEIKCPYKFRENTSTEMINDCSSYLHPEGKLNRNHKFAEFIVQAKDKLIVDTIKRDDDFITQTISHLNEFWKKHIFPELVTGRLRTLLKEKEKIKYSVNLGELVCYCQKHWDQNDHSPSNQLIGCDSSNCPYKWIHLGCVRPRRKTVPKGIWYCKECRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YCN8</accession>
    <name>A0A9Q0YCN8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_42453</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000075">
        <property type="protein sequence ID" value="KAJ8019149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YCN8"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8019149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="70" mass="8234" checksum="79105B8E72E23981" modified="2023-09-13" version="1">MESSREFNFNAAFLELSRSENCLRYVPIIAATWQHFEEAAIIVIQLLCVLEVSIKERSRNCWTVYTRSFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YDV5</accession>
    <name>A0A9Q0YDV5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_40722</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020977.1"/>
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            <begin position="30"/>
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            <dbReference type="PROSITE" id="PS50038"/>
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        <source>
            <dbReference type="EMBL" id="KAJ8020977.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="327" mass="36277" checksum="12B38B45A8F70FAD" modified="2023-09-13" version="1">MGTTSLGTTAQAPNVCPNFQTSLDDGVQGCISSPLPACRSINLCETFLPNRFNLGDSLTKLQERYNEYTNKLNITCHPYAIRFLCFRFSPPCPKVTIEETSEPYYAFDIINFPHVDIPLPCKSLCENISETCIEDEPERKDLSSRCSTLPESTSEEICFGSALAQVSLCNSFTCENGGSCFTVNDRQYCKCQPGYFGTHCEVNTSSDYRVIQGHCVSVPEQCRSVLPYNATRGLNLSDSHLAYIAGWERIAPMAYEGIMDTRVILLFCGLFYPPCAANESGSTSIVCWDLCILTKLQYEFILASLGFPTLLCEKLHKEAPPGYTCLS</sequence>
</entry>
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    <accession>A0A9Q0YEY4</accession>
    <name>A0A9Q0YEY4_HOLLE</name>
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        <submittedName>
            <fullName evidence="3">GRB10-interacting GYF protein 2</fullName>
        </submittedName>
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    <gene>
        <name evidence="3" type="ORF">HOLleu_38839</name>
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    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
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    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
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    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="GYF"/>
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        <property type="entry name" value="GYF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035445">
        <property type="entry name" value="GYF-like_dom_sf"/>
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        <property type="entry name" value="FI03272P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14445">
        <property type="entry name" value="GRB10 INTERACTING GYF PROTEIN"/>
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        <property type="entry name" value="GYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00444">
        <property type="entry name" value="GYF"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GYF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50829">
        <property type="entry name" value="GYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GYF" evidence="2">
        <location>
            <begin position="589"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="1414"/>
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            <begin position="120"/>
            <end position="142"/>
        </location>
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    </feature>
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        <location>
            <begin position="175"/>
            <end position="200"/>
        </location>
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            <begin position="226"/>
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            <begin position="239"/>
            <end position="285"/>
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        <location>
            <begin position="328"/>
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        <location>
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        <location>
            <begin position="1245"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1268"/>
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        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
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        <location>
            <begin position="1414"/>
            <end position="1423"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8021592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1464" mass="165992" checksum="67CD3EC53BF098A9" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-04-02" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YFR5</accession>
    <name>A0A9Q0YFR5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Krueppel-related zinc finger protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_44527</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000967">
        <property type="protein sequence ID" value="KAJ8017821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="5988713at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.960.10">
        <property type="entry name" value="VSR Endonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33206:SF1">
        <property type="entry name" value="DNA-DIRECTED DNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33206">
        <property type="entry name" value="PROTEIN CBG10425"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="3"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="32"/>
            <end position="59"/>
        </location>
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        <location>
            <begin position="61"/>
            <end position="88"/>
        </location>
    </feature>
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        <location>
            <begin position="83"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8017821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1509" mass="176230" checksum="08EC0DC6FFB8C46D" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YHA3</accession>
    <name>A0A9Q0YHA3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Serine incorporator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_40093</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TDE1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YHA3"/>
    <dbReference type="OrthoDB" id="5963193at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005016">
        <property type="entry name" value="TDE1/TMS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10383">
        <property type="entry name" value="SERINE INCORPORATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10383:SF9">
        <property type="entry name" value="SERINE INCORPORATOR, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03348">
        <property type="entry name" value="Serinc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040308441" evidence="7">
        <location>
            <begin position="20"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="103"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="137"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="165"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="207"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="245"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="278"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="329"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="406"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="447"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8020489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="473" mass="51682" checksum="A28076500E88397F" modified="2023-09-13" version="1">MCIGCGLASLACCVTSTACSCCCAACPSCKNSTSTRIMYGILLVVGAIVCILMVTPAVQDGLAKIPFLCANDTQLISTLRDVFSLPPNAATQGVCSLVVGYKAVYRVCFGMTCFFVLLSLIMVCVRSSKDPRSGIQNGFWFFKVLILTAIIVASFWIPYGTFEEVWRYFGIIGSFLFILIQLILIIDFAHSWNESWKAKMDDSESKGWFCALMTVTVFMFLIAITGTVLYYVFYVGHGTDSGCGLHKFFISFNLIACVGITVLSILPFIQEVVPHSGILQPSVISVYTTYLVWSAMSSNPDQDCNPGLAQIVHLNATTAEPLGVSGEDWVGMFVLLFCVVYASIRTATTSNVGKLTGNGDDKADYGSGEKVLLDDDAKDVEKASGGDEDQKVWDDEEDGVSYSYSFFHFMLALASCYVMMTLTNWFNPHFTDKSALLAGTGAMWVKISSSWACLGLFVWTLVAPLILKNRDFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YIF3</accession>
    <name>A0A9Q0YIF3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Doublecortin domain-containing protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_40565</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YIF3"/>
    <dbReference type="OrthoDB" id="1738954at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005815">
        <property type="term" value="C:microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17071">
        <property type="entry name" value="DCX1_DCDC2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16113">
        <property type="entry name" value="DCX2_DCDC2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.230:FF:000004">
        <property type="entry name" value="Doublecortin domain containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.230">
        <property type="entry name" value="Doublecortin domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003533">
        <property type="entry name" value="Doublecortin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036572">
        <property type="entry name" value="Doublecortin_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23004">
        <property type="entry name" value="DOUBLECORTIN DOMAIN CONTAINING 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23004:SF11">
        <property type="entry name" value="PROTEIN RPI-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03607">
        <property type="entry name" value="DCX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00537">
        <property type="entry name" value="DCX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89837">
        <property type="entry name" value="Doublecortin (DC)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50309">
        <property type="entry name" value="DC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Doublecortin" evidence="3">
        <location>
            <begin position="13"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="Doublecortin" evidence="3">
        <location>
            <begin position="137"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="221"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="319"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="352"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="417"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="277"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="319"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="352"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="367"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="455"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="466"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8020862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="478" mass="54159" checksum="03AEED14B1C09F2E" modified="2023-09-13" version="1">MATAVSQSQSNAINIHVFRNGDSHYKGRKFVVNQRQTRNFDAFLSMVTTGVKPPYGAVRKLYTPTHGHKVDDLSRLENNKYYVAAGMERFRRVQYFSIGRPDGAPRQTSILQRSIKPVAHSRIQASAKISKPADGPKTIHVYRNGDTLYPSVRLLLTKRMLSNWDVVLQVITERVNLINGAVRKLWHMDGSRVIELSEIENDTSYVASGNDKFKKVAYGINGPLEPSSPRIKRPAPPKQKPIISRKPTKITQKVTPPKKVPKKPPQPKPEKKKKKEKTAEQKEAETVIRSKPTMVKRSREKDESDVLDFGENDKSVFKAKEGRKETKGAAEIQDSQETAIDLPIDQIAAEEVEEEVAPSQDVDPEEDTYRKEDTIPREKTFENTLANSFSEPKKDVVDDYPDNSFSEFKENTAQDLFEETPADNNIGTVENEDDQKREIEPATRSGYEVVEEETEKNADKDRTTPTEEQLTAFLTDQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YIM5</accession>
    <name>A0A9Q0YIM5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_41017</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8019426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YIM5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8019426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="89" mass="9842" checksum="2188B9E83FC79DB1" modified="2023-09-13" version="1">MVAASYAYAGMVSWYPFIDGSSLLCLLKNGVMGFVYNPSPHSILTLYPTGILNPLYLHPTSFSCTDPGYGPESTFVRKGVLISYLFFFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YIV6</accession>
    <name>A0A9Q0YIV6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">G-protein coupled receptor moody</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_39630</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8022215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YIV6"/>
    <dbReference type="OrthoDB" id="10044919at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00637">
        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24228">
        <property type="entry name" value="B2 BRADYKININ RECEPTOR/ANGIOTENSIN II RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24228:SF72">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="42"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="78"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="118"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="158"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="204"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="277"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="303"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="58"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8022215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="359" mass="40163" checksum="B8C9026E4486148A" modified="2023-09-13" version="1">MEVGSTETPSVDGPPTHSSPWSNQGVVPWSDDFVFDNPTQRIIIAIAACLITVIGIPGNIMVILAVMLSKKLQNKTNVFVVNLACADMLTCAVLPFQAVSLLNETPPLPSFLCRMVAIFVWLGVGSSVVNLALIAFIRYSLITKSRSYSDRLLTKPKLSLFICGAWFVPFLIIVLPLPFDVGYLGYSPTYKMCTGYWKDPLSEISTVIDLFIAFLCLIVIMLCYVKIYRFVRNAGRNLIQPRNSITINEDPESSRTAPLTSTEVAIFRRQVEVTKNLFIVVCTYVICVMPYGFSNIPGPKNPFVPWTILLLYMNSCLNPIIYGLKHPQFKEVFWAIITCSWRNIPEPSLVVSFFCNIST</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YIW4</accession>
    <name>A0A9Q0YIW4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Monocarboxylate transporter 12</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_40914</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8021132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YIW4"/>
    <dbReference type="OrthoDB" id="8055603at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008028">
        <property type="term" value="F:monocarboxylic acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF303">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="169"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="206"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="235"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="260"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8021132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="360" mass="39053" checksum="ADA6E7A5718D5917" modified="2023-09-13" version="1">MGLLGGFLFGLGFFCVGLFGGQLWHFFIFMGTTAVGVGLLDFASFMSFIVHFKTNYQSLNSIRKLFAYVGISLLPPLVELFRSKFGFNGSYLILGAICWNGVVCALLLKDDKTHQKRSCSHSSEKKSYDLLNGKTSGDSHKDQDTQTKDKGELIVCNLRLLSSVTSHPLYSGLILTEGLLYYNFVIWAVCLVPYGRSVGLKPSAAVFLSTAGGFGGLVGTLVAFLVCYVDKMNPISAFLVPALLCNCGVFGYIITKSSLFKYLFSSICGFSFAFADTALSGMVPRYVCSYHLRQGAAIGYIVTGTFMQLGGIISGQSLKYKKVLTPSLPPTFPDTKMASATQKSCADILNCYDSWSSTPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YJF0</accession>
    <name>A0A9Q0YJF0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_41647</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8019876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YJF0"/>
    <dbReference type="OrthoDB" id="5986179at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="13"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="41"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8019876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="112" mass="12125" checksum="85FF26AC2DBC3512" modified="2023-09-13" version="1">MLTITRPTNVVKEDNALYSPGHPDLGDCSPKSTGASRKRTGSSTSQTSTKKNIRLSAFDVSELVVQRNIKTRTQVLVLAKQQKQEGKTDFAEFIVNGGGGGGEQNVMIQPFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YKH5</accession>
    <name>A0A9Q0YKH5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL30</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">60S ribosomal protein L7</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_39983</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL30 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YKH5"/>
    <dbReference type="OrthoDB" id="28644at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01657">
        <property type="entry name" value="Ribosomal_L7_archeal_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1390.20:FF:000002">
        <property type="entry name" value="60S ribosomal protein L7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1390.20:FF:000003">
        <property type="entry name" value="60S ribosomal protein L7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1390.20">
        <property type="entry name" value="Ribosomal protein L30, ferredoxin-like fold domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036919">
        <property type="entry name" value="Ribo_uL30_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039699">
        <property type="entry name" value="Ribosomal_uL30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018038">
        <property type="entry name" value="Ribosomal_uL30_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005998">
        <property type="entry name" value="Ribosomal_uL30_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035808">
        <property type="entry name" value="Ribosomal_uL30_euk_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016082">
        <property type="entry name" value="Ribosomal_uL30_ferredoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012988">
        <property type="entry name" value="Ribosomal_uL30_N_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01310">
        <property type="entry name" value="uL30_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11524">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11524:SF16">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00327">
        <property type="entry name" value="Ribosomal_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08079">
        <property type="entry name" value="Ribosomal_L30_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55129">
        <property type="entry name" value="Ribosomal protein L30p/L7e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00634">
        <property type="entry name" value="RIBOSOMAL_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 9" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL30 N-terminal eukaryotes" evidence="8">
        <location>
            <begin position="12"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL30-like ferredoxin-like fold" evidence="7">
        <location>
            <begin position="89"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00040575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00041271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8020401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="246" mass="29267" checksum="3AF0628543142252" modified="2023-09-13" version="1">MADDNKDRKPVVPETILKRRKQRELRKARELQKKIKAKKDKYKKRKEIFYRAEKYALQYKKQEEDKVRLAREARRYGNYYVPDEPRLALVIRIRGINGVSPRVRKILQLLRLRQIQNAVFVRLNKATLNMLHLVEPYIAWGYPSLKTVRELIYKRGYGKVQGSRLPLSDNAMIEKALGKRNIICIEDLIHEIFTVGDNFKQANNFLWPFKLNPPRGGYRKKGIHWVEGGDFGNRETYINRLMRQMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YMJ8</accession>
    <name>A0A9Q0YMJ8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_35813</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MS4A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YMJ8"/>
    <dbReference type="OrthoDB" id="2019149at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007237">
        <property type="entry name" value="CD20-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030417">
        <property type="entry name" value="MS4A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23320:SF165">
        <property type="entry name" value="MARVEL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23320">
        <property type="entry name" value="MEMBRANE-SPANNING 4-DOMAINS SUBFAMILY A MS4A -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04103">
        <property type="entry name" value="CD20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="71"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="100"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="138"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8023384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="220" mass="23849" checksum="C4663513EF6A3588" modified="2023-09-13" version="1">MNAGSQQVILGNQQHPHVGPFVNPTRPDGRLWGSNVRRSMGIIQIICGGIEFVLGIALICLPTDYFDHIDYVGWGIWTGVFAIVTGFLGIFSLTKRCMVIAYMITSIITAVLCTGCFLYAVFGVLIGSYGTNGTANLALYIILCIVFFIHMVISIIGASFTCGALISSTNQPQQVVYYTPQPLHQQYGPTPPYTEAAPHSGITSQTTTPSTNEAFISQKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YP32</accession>
    <name>A0A9Q0YP32_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">DNA-directed RNA polymerase III subunit RPC4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_37593</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YP32"/>
    <dbReference type="OrthoDB" id="5836119at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007811">
        <property type="entry name" value="RPC4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13408">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13408:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05132">
        <property type="entry name" value="RNA_pol_Rpc4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 6" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="207"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="306"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="64"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="76"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="92"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="106"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="126"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="138"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="165"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="326"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8022636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="428" mass="46910" checksum="14F28751BB532818" modified="2023-09-13" version="1">MAENNKPSSSSSTAPIKLDTLTRGLVGRKHDLMNPTARLPSLRTPRDLTLKNAVKKSFKPNIPVRRERPKEDGDAGSRSSSPGPSKIPKGNGRGRGRGKRGAGARGGRDRNKELIQTHNSIFEEGPGDRLVRRSTTEGFGRSSVSSRGDSVSSGSLASSFRSLNLKREEREEEDSKRIAELLRDDFVKDGRLTVDDRYKPISLPLKTMAASSSSSSSSSLQDITDETDASGISIKQEPMSPPPMDVKVKQEPPDSDEEMPLVDEKKDVKPKIFTKVKGSEPKKSTLPLFSRRDALEGKLLFFQLPDSLPALPPTEDEDRSLRGRSQEVQGTSSQGTPEVQSQKTVKNECTLNDLPAGFLGKIRIRKSGKTELRLGNITLDVSVGTPCGFLQDVVSVKNVQDHQEIINLGPVKHRIICTPNLMSLLENR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YPG4</accession>
    <name>A0A9Q0YPG4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">receptor protein-tyrosine kinase</fullName>
            <ecNumber evidence="2">2.7.10.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">HOLleu_35244</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">Nanhai2018</strain>
            <tissue evidence="21">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="2"/>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8025125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="5979328at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004714">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000554">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050122">
        <property type="entry name" value="RTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416:SF600">
        <property type="entry name" value="PDGF- AND VEGF-RECEPTOR RELATED, ISOFORM J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12 21" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1066"/>
            <end position="1089"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="12"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="440"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="636"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="972"/>
            <end position="1057"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="1142"/>
            <end position="1586"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1245"/>
            <end position="1289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1305"/>
            <end position="1376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1610"/>
            <end position="1653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1250"/>
            <end position="1261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1262"/>
            <end position="1272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1280"/>
            <end position="1289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1331"/>
            <end position="1343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1352"/>
            <end position="1376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1616"/>
            <end position="1626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1627"/>
            <end position="1641"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="1176"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00051243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAJ8025125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1653" mass="185095" checksum="7F5BB8B7333B731B" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YPU4</accession>
    <name>A0A9Q0YPU4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tyrosine-protein kinase ephrin type A/B receptor-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_38090</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8023026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YPU4"/>
    <dbReference type="OrthoDB" id="205145at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319:SF35">
        <property type="entry name" value="OUTER MEMBRANE PROTEIN PMPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="169"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="235"/>
            <end position="256"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="1">
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            <begin position="276"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
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            <begin position="377"/>
            <end position="395"/>
        </location>
    </feature>
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            <begin position="451"/>
            <end position="475"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAJ8023026.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="503" mass="59172" checksum="F8306D5DCCC614BB" modified="2023-09-13" version="1">MGGIGCKTCNNGTFIKEGSGRSALSCKVCPGGTDHTRPAGFRACFCLENFYRTDRFDQCFPCPNNGLQCSNDHVRIKQHYYWYWESNLKSAYQHFIVNILIRNDSYDRQWSMFQGPLPKAYKCPVEENCVNDNSEVEGNCREGYRGWMCTICDDDYFELFGWCQKCHPFLAVGIIILLLLLVSFLWFVIIQPVSAPRTTRGKFISDMIISRFKIMFGFYQVVESYWFHMGTHVRFAPIQLAISRIVGVMSFTLSSILVSPKCYISWFQWNPYTELWTVYLTLALFTCIVVLTYSVVRGAIRREWIGWSVDSVRTACLKLFVFTLFASYTEICDVVFKLYNCDEFSLYEYQEIPKVRFLHSHYNISCDDAIYKRYRRFYAVPGWIFLCSFPMIMWTCLYKCSRFQRDCNVTVDRYYPEWLRFMCENYKPQCWYWEIVELGRKILLTFLPLAFGWKGIFVLAGLFASGLFLALHAYVRPMKHSWDNTLQVSLETETFKQSFTLLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YT32</accession>
    <name>A0A9Q0YT32_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Hermansky-Pudlak syndrome 1 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_34009</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8026217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="10255234at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031085">
        <property type="term" value="C:BLOC-3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043971">
        <property type="entry name" value="FUZ/MON1/HPS1_longin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043970">
        <property type="entry name" value="FUZ/MON1/HPS1_longin_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026053">
        <property type="entry name" value="HPS1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12761:SF1">
        <property type="entry name" value="BLOC-3 COMPLEX MEMBER HPS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12761">
        <property type="entry name" value="HERMANSKY-PUDLAK SYNDROME PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19037">
        <property type="entry name" value="Fuz_longin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19038">
        <property type="entry name" value="Fuz_longin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FUZ/MON1/HPS1 second Longin" evidence="2">
        <location>
            <begin position="199"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="FUZ/MON1/HPS1 third Longin" evidence="3">
        <location>
            <begin position="577"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="282"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="338"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="361"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8026217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="738" mass="83515" checksum="EA5A9539F145D1BD" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BAS7</accession>
    <name>A0A9Q1BAS7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Reverse transcriptase RNase H-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_40810</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8021051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BAS7"/>
    <dbReference type="OrthoDB" id="427924at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09274">
        <property type="entry name" value="RNase_HI_RT_Ty3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000063">
        <property type="entry name" value="Zinc knuckle domaincontaining protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041373">
        <property type="entry name" value="RT_RNaseH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF10">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17917">
        <property type="entry name" value="RT_RNaseH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="222"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase RNase H-like" evidence="8">
        <location>
            <begin position="129"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8021051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="241" mass="27610" checksum="A12A7BDEB0B56460" modified="2023-09-13" version="1">MAHHRLVVGEVLSRLKRYHIRIKPSECEWFKPSVVYLGHKVDGDGLHPTQNKADDLLNAPTPSNVSELQSYLRLLNFYGKFLPNLSTLLHPLHKLLCKNVPWVWTEACDVAFQQSKQHLLQSQLLVHYDVNKELRPACMRQLMGSGQSFRNGEERPIAFASRTLSKSEKNYAQIEREALSLIFGVKKFHKYLYGRMFTIITDHKPLTAILISKVPHTHISGCNLLILFLLVLSLFSSIIVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BDJ9</accession>
    <name>A0A9Q1BDJ9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ficolin-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_38709</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BDJ9"/>
    <dbReference type="OrthoDB" id="7250310at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00087">
        <property type="entry name" value="FReD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19941">
        <property type="entry name" value="TIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050373">
        <property type="entry name" value="Fibrinogen_C-term_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036084">
        <property type="entry name" value="Ser_inhib-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025615">
        <property type="entry name" value="TILa_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040941">
        <property type="entry name" value="GGGWT_bact"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143:SF458">
        <property type="entry name" value="FIBRINOGEN C-TERMINAL DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143">
        <property type="entry name" value="FIBRINOGEN/TENASCIN/ANGIOPOEITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12714">
        <property type="entry name" value="TILa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00186">
        <property type="entry name" value="FBG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57567">
        <property type="entry name" value="Serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="3">
        <location>
            <begin position="50"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="279"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="3">
        <location>
            <begin position="316"/>
            <end position="551"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8021483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="551" mass="62477" checksum="0CBBD2160B62A201" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BEZ1</accession>
    <name>A0A9Q1BEZ1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Calcium uptake protein 1, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HOLleu_38052</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Nanhai2018</strain>
            <tissue evidence="17">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the MICU1 family. MICU1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BEZ1"/>
    <dbReference type="OrthoDB" id="10056860at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990246">
        <property type="term" value="C:uniplex complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036444">
        <property type="term" value="P:calcium import into the mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051560">
        <property type="term" value="P:mitochondrial calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15900">
        <property type="entry name" value="EFh_MICU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039800">
        <property type="entry name" value="MICU1/2/3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12294:SF1">
        <property type="entry name" value="CALCIUM UPTAKE PROTEIN 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12294">
        <property type="entry name" value="EF HAND DOMAIN FAMILY A1,A2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13202">
        <property type="entry name" value="EF-hand_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13833">
        <property type="entry name" value="EF-hand_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="38"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="16">
        <location>
            <begin position="209"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="16">
        <location>
            <begin position="400"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="67"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="67"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ8022998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="465" mass="53209" checksum="256C32D515D951EE" modified="2023-09-13" version="1">MFRIITRRTLQVIAQHQWRNVTSSAKQTSESNAWPVKYMVALLGAGGGIVTGGMLWERSMHAAVKTVEEENSKEVTEAATDEEDSPEDGEKKKKKGPGFRDRKVIEYENRIRAYSTPDKIFRYFATLRAVLDNGESEIYMTPEDFIRSITPDEKQPDGLGLDQFKKIDPGRYKATSEHSWDEDSIFNILGDCGLISFTDYMFLLTVLSTPERHFEIAFRLFDLNGDGEVQVDEFQQVTDVIRAQTATGKRHRDRTTTGNVIGKIPHSSLTTYFFGKNLDKKLTIADFLAFHRKLRKDLLKLEFNKFEPEDGRIQERCLAKFLIVHANLPQQKRSRMVKRVKKRFAEDPKGITFEELEALQKFLSNVNDINTALSFYHVAGADIDPVTLRHVAKMVGGSDLSERVVEVIYTMFDENDDGKLSHKELISVMKHRLQRGLDKSKELGLSRILEAMWKCAKKTSTGYFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BFH7</accession>
    <name>A0A9Q1BFH7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">PH and SEC7 domain-containing protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_37709</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BFH7"/>
    <dbReference type="OrthoDB" id="2157641at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032012">
        <property type="term" value="P:regulation of ARF protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00171">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1000.11:FF:000002">
        <property type="entry name" value="Cytohesin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1000.11">
        <property type="entry name" value="Arf Nucleotide-binding Site Opener,domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041681">
        <property type="entry name" value="PH_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001605">
        <property type="entry name" value="PH_dom-spectrin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023394">
        <property type="entry name" value="Sec7_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000904">
        <property type="entry name" value="Sec7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035999">
        <property type="entry name" value="Sec7_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663">
        <property type="entry name" value="GUANYL-NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663:SF376">
        <property type="entry name" value="PH AND SEC7 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15410">
        <property type="entry name" value="PH_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01369">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00683">
        <property type="entry name" value="SPECTRINPH"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00222">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48425">
        <property type="entry name" value="Sec7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50190">
        <property type="entry name" value="SEC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SEC7" evidence="7">
        <location>
            <begin position="296"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="6">
        <location>
            <begin position="506"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="75"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="223"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="275"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="465"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="711"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="645"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="91"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="236"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="282"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="467"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="724"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="757"/>
            <end position="766"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8022727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="784" mass="86797" checksum="100344CFF5D0C0F4" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BH76</accession>
    <name>A0A9Q1BH76_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DNA repair protein XRCC2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_35192</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8025084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BH76"/>
    <dbReference type="OrthoDB" id="420422at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033063">
        <property type="term" value="C:Rad51B-Rad51C-Rad51D-XRCC2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005657">
        <property type="term" value="C:replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000400">
        <property type="term" value="F:four-way junction DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042148">
        <property type="term" value="P:DNA strand invasion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19490">
        <property type="entry name" value="XRCC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020588">
        <property type="entry name" value="RecA_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030547">
        <property type="entry name" value="XRCC2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46644">
        <property type="entry name" value="DNA REPAIR PROTEIN XRCC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46644:SF2">
        <property type="entry name" value="DNA REPAIR PROTEIN XRCC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50162">
        <property type="entry name" value="RECA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RecA family profile 1" evidence="2">
        <location>
            <begin position="16"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="256"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="268"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8025084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="350" mass="38922" checksum="FB6F8F084572AFAD" modified="2023-09-13" version="1">MNSMAGGSETGAQLFARLGSRPSLKALQNSLFGEVYPKPGDLIELSGESGTAKTEILMNITVSCILPLEWKTYKLGGIGTGVVFVDTQCQFSMLRVFSLLEKRVTRCIASSHSSKVHGKCSEQDIQTVDKIVNSKLEDDGHATDKVKEHSCPPTAEEIENFIKTCLEKLLIFRCNTSQQLLVTFHSLETLFANSLDISVLMVDNWSAFYWMDRASAGNHLSQVETIQQRMISSLEKLLRDYHITAFVAKRPLFKSSVKRDKGPPTSGPSPSLSSSNLQPDDPKPSNQDFVSKDWNRILSQKFQIQMSEQPISTISSEVNLQTLFVASRIFPKPVITKTFFVSEIGLNFLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BIM4</accession>
    <name>A0A9Q1BIM4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">(S)-2-hydroxy-acid oxidase</fullName>
            <ecNumber evidence="2">1.1.3.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_32401</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>2-hydroxyoctanoate + O2 = 2-oxooctanoate + H2O2</text>
            <dbReference type="Rhea" id="RHEA:67940"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:133514"/>
            <dbReference type="ChEBI" id="CHEBI:176689"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:67941"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a (2S)-2-hydroxycarboxylate + O2 = a 2-oxocarboxylate + H2O2</text>
            <dbReference type="Rhea" id="RHEA:16789"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="ChEBI" id="CHEBI:58123"/>
            <dbReference type="EC" id="1.1.3.15"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:16790"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.3.15" evidence="2"/>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BIM4"/>
    <dbReference type="OrthoDB" id="25826at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003973">
        <property type="term" value="F:(S)-2-hydroxy-acid oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001561">
        <property type="term" value="P:fatty acid alpha-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02809">
        <property type="entry name" value="alpha_hydroxyacid_oxid_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000056">
        <property type="entry name" value="hydroxyacid oxidase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012133">
        <property type="entry name" value="Alpha-hydoxy_acid_DH_FMN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000262">
        <property type="entry name" value="FMN-dep_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037396">
        <property type="entry name" value="FMN_HAD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578:SF149">
        <property type="entry name" value="2-HYDROXYACID OXIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578">
        <property type="entry name" value="S -2-HYDROXY-ACID OXIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01070">
        <property type="entry name" value="FMN_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000138">
        <property type="entry name" value="Al-hdrx_acd_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01240">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51349">
        <property type="entry name" value="FMN_HYDROXY_ACID_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0288">FMN</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FMN hydroxy acid dehydrogenase" evidence="9">
        <location>
            <begin position="1"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="273"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="80"/>
            <end position="82"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="249"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="271"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="273"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="276"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="305"/>
            <end position="309"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="328"/>
            <end position="329"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00013087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000138-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000138-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8027292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="384" mass="41899" checksum="7EC7F772671834D9" modified="2023-09-13" version="1">MPTEGVSRVADFEGLVRDKTPKVLADYYATGAGDEQTLEDSEKAFRRFRLRPRVLWSKENINIATKLQGQEVSFPLGISPTGLQLAAHSEGERATARAAAKAGVVMIFSSWAMVHLEEAAAAAPNATLWMQVAPFRDRRVTIDMVKRAERSGFKAIVVTVDLPATGMYKRTFRTGTQAQKQLESLNVPGLLLISVRMVNFQAVKEDVAKAKASGDKYLLEYAVLQTNSPGSWEYISWIKSLTTLPIILKGIVTAESAREAMAAGVQGIIVSAHGGRQLDGIQAPIEALPEVVDALRGSGIEVYMDGGVRSGRDIFKALAIGAKAVFIGRPVIWGLLHNGEDGVTEILHMLKNEFQNTMSLCGCSRVDDVNRTFIRHESQLTCKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BJ52</accession>
    <name>A0A9Q1BJ52_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Slit-like 2 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_32759</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BJ52"/>
    <dbReference type="OrthoDB" id="676979at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050541">
        <property type="entry name" value="LRR_TM_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369">
        <property type="entry name" value="ANTIGEN BSP, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369:SF210">
        <property type="entry name" value="CHAOPTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="353"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8027580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="400" mass="45694" checksum="A2C17643ACD6A1FA" modified="2023-09-13" version="1">MKTDPMVSTCSQICEYEVFFHRAHCDQRALSFVPRSSGCENVSLLELQGNNITAITNEQMSGYLHVRTLDISRNQISSLEPGTFHSNNHLRNVLISHNNLDILRNYTFNGTEKDLQRIYLDNNKLRMIEKGALEGLTKLKILRLERNFLTYIPEGLLFGLFVLHQVNLSFNQLNDTAFPENFGFHNLPILDVRYNHFHRCSSIVPYLYNVTDMLHVDGNPFLCDCNCKIIQKWLDETYASGERTPSAKVPVTCKSSNGTYVGVTESLALNCDDTVGINTTPSQTTLSHFHPVLSDADLITTRKDNIKQLTGHGKEANISLQWDHVDHIGGPTQAQQNKGVTLKELYRAHWTSYYTAVILTLYFLVLSGRWARRIFTSREMQRYPENMKNRSRNISPTVCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BJW5</accession>
    <name>A0A9Q1BJW5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Proton-coupled zinc antiporter SLC30A9, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Solute carrier family 30 member 9</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Zinc transporter 9</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="26" type="ORF">HOLleu_30018</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="26">Nanhai2018</strain>
            <tissue evidence="26">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>Zn(2+)(in) + 2 H(+)(out) = Zn(2+)(out) + 2 H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:72627"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="26">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8027921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BJW5"/>
    <dbReference type="OrthoDB" id="435980at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006882">
        <property type="term" value="P:intracellular zinc ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006829">
        <property type="term" value="P:zinc ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21078">
        <property type="entry name" value="NTD_ZNT9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1510.10:FF:000004">
        <property type="entry name" value="zinc transporter 9 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="entry name" value="Cation efflux protein transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.530.10">
        <property type="entry name" value="XPA C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058533">
        <property type="entry name" value="Cation_efflux_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040177">
        <property type="entry name" value="SLC30A9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037129">
        <property type="entry name" value="XPA_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01297">
        <property type="entry name" value="CDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13414">
        <property type="entry name" value="HUEL-CATION TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13414:SF9">
        <property type="entry name" value="PROTON-COUPLED ZINC ANTIPORTER SLC30A9, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="Cation efflux protein transmembrane domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="Putative DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0050">Antiport</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="13" id="KW-0406">Ion transport</keyword>
    <keyword evidence="15 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="24" id="KW-0732">Signal</keyword>
    <keyword evidence="16" id="KW-0804">Transcription</keyword>
    <keyword evidence="12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 23" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="10" id="KW-0864">Zinc transport</keyword>
    <feature type="signal peptide" evidence="24">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040393361" description="Proton-coupled zinc antiporter SLC30A9, mitochondrial" evidence="24">
        <location>
            <begin position="29"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="289"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="350"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="393"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="438"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="471"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein transmembrane" evidence="25">
        <location>
            <begin position="291"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="70"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="135"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="70"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="85"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="146"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00033405"/>
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    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00034845"/>
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    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00034922"/>
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    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00048349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF01545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="EMBL" id="KAJ8027921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="618" mass="69032" checksum="242A3525C5E87097" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BLI8</accession>
    <name>A0A9Q1BLI8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Protein dachsous</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">HOLleu_31194</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">Nanhai2018</strain>
            <tissue evidence="19">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Cadherins are calcium-dependent cell adhesion proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cell membrane</location>
            <topology evidence="1 14">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6252479at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009887">
        <property type="term" value="P:animal organ morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007163">
        <property type="term" value="P:establishment or maintenance of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048729">
        <property type="term" value="P:tissue morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="26"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000104">
        <property type="entry name" value="cadherin-23 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000140">
        <property type="entry name" value="Dachsous cadherin-related 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000020">
        <property type="entry name" value="Dachsous cadherin-related 1b"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000116">
        <property type="entry name" value="Dachsous cadherin-related 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Dachsous, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000015">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000032">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000039">
        <property type="entry name" value="FAT atypical cadherin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000061">
        <property type="entry name" value="FAT atypical cadherin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FAT atypical cadherin 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000181">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000007">
        <property type="entry name" value="Protocadherin alpha 2"/>
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        <property type="entry name" value="protocadherin Fat 4"/>
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    <dbReference type="FunFam" id="2.60.40.60:FF:000134">
        <property type="entry name" value="protocadherin Fat 4"/>
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    </dbReference>
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        <property type="entry name" value="Putative cadherin-23"/>
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        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="27"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.900.10">
        <property type="entry name" value="TCF3-CBD (Catenin binding domain)"/>
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    </dbReference>
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        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000233">
        <property type="entry name" value="Cadherin_Y-type_LIR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027397">
        <property type="entry name" value="Catenin-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24026">
        <property type="entry name" value="FAT ATYPICAL CADHERIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24026:SF126">
        <property type="entry name" value="PROTOCADHERIN FAT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01049">
        <property type="entry name" value="CADH_Y-type_LIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="25"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="27"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="27"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="27"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 13 14" id="KW-0106">Calcium</keyword>
    <keyword evidence="8 14" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 14 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="2902"/>
            <end position="2927"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="18">
        <location>
            <begin position="73"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="18">
        <location>
            <begin position="166"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="18">
        <location>
            <begin position="282"/>
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    </feature>
    <feature type="domain" description="Cadherin" evidence="18">
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    </feature>
    <feature type="domain" description="Cadherin" evidence="18">
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        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="18">
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        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="18">
        <location>
            <begin position="710"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="18">
        <location>
            <begin position="815"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="18">
        <location>
            <begin position="919"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="18">
        <location>
            <begin position="1023"/>
            <end position="1127"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="18">
        <location>
            <begin position="1128"/>
            <end position="1229"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="18">
        <location>
            <begin position="1231"/>
            <end position="1333"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="18">
        <location>
            <begin position="1333"/>
            <end position="1438"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="18">
        <location>
            <begin position="1439"/>
            <end position="1543"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="18">
        <location>
            <begin position="1543"/>
            <end position="1647"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="18">
        <location>
            <begin position="1648"/>
            <end position="1752"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="18">
        <location>
            <begin position="1753"/>
            <end position="1856"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="18">
        <location>
            <begin position="1857"/>
            <end position="1961"/>
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    </feature>
    <feature type="domain" description="Cadherin" evidence="18">
        <location>
            <begin position="1962"/>
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        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="18">
        <location>
            <begin position="2061"/>
            <end position="2169"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="18">
        <location>
            <begin position="2170"/>
            <end position="2274"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="18">
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            <begin position="2274"/>
            <end position="2374"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="18">
        <location>
            <begin position="2375"/>
            <end position="2479"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="18">
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            <end position="2585"/>
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    </feature>
    <feature type="domain" description="Cadherin" evidence="18">
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            <begin position="2586"/>
            <end position="2690"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="18">
        <location>
            <begin position="2691"/>
            <end position="2792"/>
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    </feature>
    <feature type="domain" description="Cadherin" evidence="18">
        <location>
            <begin position="2793"/>
            <end position="2902"/>
        </location>
    </feature>
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        <location>
            <begin position="3002"/>
            <end position="3072"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="3254"/>
            <end position="3315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="3008"/>
            <end position="3017"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="3038"/>
            <end position="3054"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="3055"/>
            <end position="3072"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="3254"/>
            <end position="3291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAJ8028840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="3315" mass="362088" checksum="E4FF84782EB39A32" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BLW2</accession>
    <name>A0A9Q1BLW2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_28221</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8028959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BLW2"/>
    <dbReference type="OrthoDB" id="426210at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8028959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="155" mass="18183" checksum="BB0837ACB6027AE8" modified="2023-09-13" version="1">MSFNADKSKFLHFGPKNFSNEIVLNLYKQLVRPHFDYADQAWSPFYENDKFLLEQVQRRATRLIPEVRHLPYHVRLKHLGLTILELKRIRGNMLQVYKFLSERNPLSSCNYLKVQGNSRVRGHCKKLVKSFAKLDIRKFSFSHKLSTSGIVCLSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BNX7</accession>
    <name>A0A9Q1BNX7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">F-box only protein 36</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_26620</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BNX7"/>
    <dbReference type="OrthoDB" id="3219396at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="CDD" id="cd22106">
        <property type="entry name" value="F-box_FBXO36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="128"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="212"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8030258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="237" mass="27570" checksum="3172D96A66BF6C51" modified="2023-09-13" version="1">MASLLPSNGVLFDIQGQAPSPSKDFVQFIITLKETQSVYLDQLTSAVWRIPYLGWTTKSLRITMLHNDQTIWRSWRITLRNDYKRLLPSEQKEPVEDFAHDERLQFDVGKTFGDNILEYARRLCKGQIDYLVRMPKPLQIYIASFLDLEDVAMLSQTCKAMYEICSAEALWEQIFHHHCDTVTDEMTSLALDMGWKQMFFTNKLQLQVKLRRHQQKRTSPDGGSTFITEEVASNPEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BQ84</accession>
    <name>A0A9Q1BQ84_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_27197</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BQ84"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="137"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="186"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="215"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="239"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="271"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="324"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="337"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="371"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8030713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="380" mass="39270" checksum="59F0FE4F6769F0D2" modified="2023-09-13" version="1">MAFEMSSFLHAVIHEDLIEETQKVTKDGENERDGVNIQIGPRWDKRGDGRDNIEGDGGDEDEKDGGGGGKGNKKNGGKESDGISRKDSGNKNSKESGGKSDKEDGSKESGGKSDKNGGSKESGGKSGKNGGSKESGGKSNKQSGNKESGEKSTKRSKSKENGGKSDKNDGSKESGGKSNKQSGNKESGEKSNKQSKGKESGGKSDKNGGSKESGGKSNKQSGSRESGGKSNKQSGGKESGGKSDKNGGSKESDGKSDKQSGSKESGGKSNKKSDGKSGKISESKESGGKSGSHVKHPPDNPPWNGGDSRSDSKSKGSGGHSKSKSKDRDSHGGDSSHKHKRKHCNRKMKTRGHGSSSSKGGKENLSNPPLTREEVAIPEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BQ89</accession>
    <name>A0A9Q1BQ89_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="28">ATP-binding cassette sub-family C member 5</fullName>
            <ecNumber evidence="6">7.6.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="29">Multidrug resistance-associated protein 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="34" type="ORF">HOLleu_27332</name>
    </gene>
    <organism evidence="34 35">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="34" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="34">Nanhai2018</strain>
            <tissue evidence="34">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>(2S)-2-[5-amino-1-(beta-D-ribosyl)imidazole-4-carboxamido]succinate(in) + ATP + H2O = (2S)-2-[5-amino-1-(beta-D-ribosyl)imidazole-4-carboxamido]succinate(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66752"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:167466"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:66753"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>3',5'-cyclic AMP(in) + ATP + H2O = 3',5'-cyclic AMP(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66184"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:66185"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>3',5'-cyclic GMP(in) + ATP + H2O = 3',5'-cyclic GMP(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66188"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57746"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:66189"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>ATP + H2O + xenobioticSide 1 = ADP + phosphate + xenobioticSide 2.</text>
            <dbReference type="EC" id="7.6.2.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>N-acetyl-L-aspartate(in) + ATP + H2O = N-acetyl-L-aspartate(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66744"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16953"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:66745"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>N-acetyl-L-aspartyl-L-glutamate(in) + ATP + H2O = N-acetyl-L-aspartyl-L-glutamate(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66728"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:76931"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:66729"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>N-acetyl-L-aspartyl-L-glutamyl-L-glutamate(in) + ATP + H2O = N-acetyl-L-aspartyl-L-glutamyl-L-glutamate(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66732"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:76935"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:66733"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Basolateral cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasmic granule</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Endosome membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="20">Golgi apparatus lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="34">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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    <sequence length="1426" mass="159752" checksum="5D5A04887A83F72F" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BQL2</accession>
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        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
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            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
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                <person name="Chen T."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain evidence="2">Nanhai2018</strain>
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        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="54" mass="5937" checksum="1A50C13928DCE629" modified="2023-09-13" version="1">MGSKTLDKPLRVLSFRASSFYFIMILSTVSAMQNDIPRYNTTLPGATSQVNVAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BRC8</accession>
    <name>A0A9Q1BRC8_HOLLE</name>
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        <submittedName>
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        <name evidence="7" type="ORF">HOLleu_27947</name>
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            <taxon>Holothuriidae</taxon>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain evidence="7">Nanhai2018</strain>
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        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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        <property type="protein sequence ID" value="KAJ8031269.1"/>
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        <property type="entry name" value="EGG PEPTIDE SPERACT RECEPTOR"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="SCAVENGER RECEPTOR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
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    </dbReference>
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    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
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        </location>
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        </location>
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            <begin position="222"/>
            <end position="322"/>
        </location>
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            <begin position="325"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="6">
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            <begin position="430"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="6">
        <location>
            <begin position="533"/>
            <end position="633"/>
        </location>
    </feature>
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            <end position="738"/>
        </location>
    </feature>
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            <end position="841"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="6">
        <location>
            <begin position="846"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="6">
        <location>
            <begin position="949"/>
            <end position="1049"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="6">
        <location>
            <begin position="1054"/>
            <end position="1154"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="39"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="52"/>
            <end position="113"/>
        </location>
    </feature>
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            <end position="93"/>
        </location>
    </feature>
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            <end position="206"/>
        </location>
    </feature>
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        </location>
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            <end position="727"/>
        </location>
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            <begin position="707"/>
            <end position="717"/>
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    </feature>
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        <location>
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            <end position="830"/>
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            <begin position="779"/>
            <end position="840"/>
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        <location>
            <begin position="810"/>
            <end position="820"/>
        </location>
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        <location>
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        </location>
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        <location>
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        </location>
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            <begin position="974"/>
            <end position="1038"/>
        </location>
    </feature>
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        <location>
            <begin position="987"/>
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        </location>
    </feature>
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        <location>
            <begin position="1018"/>
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    </feature>
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        <location>
            <begin position="1123"/>
            <end position="1133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8031269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1161" mass="121677" checksum="803BAA5B9F2764FA" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BSM1</accession>
    <name>A0A9Q1BSM1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_25567</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BSM1"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8032132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="189" mass="20890" checksum="AFEC90A146033760" modified="2023-09-13" version="1">MPHIERKNPIVFGGGQRSSGVTGGQTPKTLLTRYLEMRYGERKNPIVFDGGQRSSGVTGGRMLKTLLTRYLKMRYGKRKNPIVCGGGQRSSGVTGGQTLKTLLTRYLKMRYGKRKNPIIFDGGQRSSGVTGGRMLKTLLTRYLKMRYGKRKNPIVCGGGQRSSGVTGGQILKTLLTRYLKIGSLDKFHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BTK3</accession>
    <name>A0A9Q1BTK3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">G-protein coupled receptor moody</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_25910</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
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        </lineage>
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    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="OrthoDB" id="10039923at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24228">
        <property type="entry name" value="B2 BRADYKININ RECEPTOR/ANGIOTENSIN II RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24228:SF72">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="20"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="58"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="94"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="137"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="189"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="265"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="37"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8032394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="347" mass="39156" checksum="47B78CD2F938CDE1" modified="2023-09-13" version="1">MEATQDFNNERYRIPDNTDAFFLGLLSVYIFLVGVPGNTLILLAVLFSRKLQTTSNAFIVNLAIADLFACLVQPLHMVGTWANYYSSSLDSMCLVASCISHLCIACSIFTLAAIAFNRFVLVVCSSSTYQSIYRSKFLKIWIPLLWTLAVLMTLLPPLALDIGKFGFDNNTHTCRSIIDYPTADQYDSIIVFGVYPLPFFIIIVCYSGILLTVIKHDRRLKKRKTEMSVISSDDPIQRSATVLTKTITKQLSVTSMEIRVTANMFIIFCAFIVCTTPLFICTIYQCLIMPYVRTVLAINSTLNPLIYGVNHPIFRSVFTSFLKCKPVPEPSNFLIGLRRRIYALRES</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BUE2</accession>
    <name>A0A9Q1BUE2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_22812</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8032766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BUE2"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8032766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="362" mass="40994" checksum="2E166B0F58588C7C" modified="2023-09-13" version="1">MGQFISVVGPVGSSALVFFHFIDTTHGLNATVFGKPEPTDLTPGGALKDDYERKFYIAGVTILVAFVLFMVYGALHEVDEDIIDYDGRRRRKKERRRLRRQRRRMARPHPCETGIYGAKHPEYDLLISMRTQYGCKCLCTCNIHSHLSTPGSKTLGTAARKGSLSESCKVVSDENKENIHLTDNPTHSELYFNESTRLSENNNSNAKRIFQSSDPARKSYDPHEEDVEIWIKRHSYLGGERTKHICVDSDESESDGASDEDLLLYESDGWTLSHLQQKTKPDVPSQGTARLTKWRSKFGKRQESADEDINLITFSSIEESPLHAKRITTGNIKERDTSKAMTSEDVWVKRTLVDDIYGNSAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BUH6</accession>
    <name>A0A9Q1BUH6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Hemicentin-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HOLleu_22945</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Nanhai2018</strain>
            <tissue evidence="15">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8032874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="446173at2759"/>
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        <property type="component" value="Chromosome 11"/>
    </dbReference>
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        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="CDD" id="cd00057">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="CDD" id="cd22823">
        <property type="entry name" value="Gal_Rha_Lectin"/>
        <property type="match status" value="6"/>
    </dbReference>
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        <property type="entry name" value="Contactin-associated protein-like 4 isoform 1"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Unc-5 netrin receptor C"/>
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    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.740">
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
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    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
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    </dbReference>
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        <property type="entry name" value="Immunoglobulins"/>
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    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="6"/>
    </dbReference>
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        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="17"/>
    </dbReference>
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        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052065">
        <property type="entry name" value="Compl_asym_regulator"/>
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    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="CTDL_fold"/>
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        <property type="entry name" value="FA58C"/>
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    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006585">
        <property type="entry name" value="FTP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
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    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
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    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
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    <dbReference type="InterPro" id="IPR000922">
        <property type="entry name" value="Lectin_gal-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043159">
        <property type="entry name" value="Lectin_gal-bd_sf"/>
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    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025155">
        <property type="entry name" value="WxxW_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040941">
        <property type="entry name" value="GGGWT_bact"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00754">
        <property type="entry name" value="F5_F8_type_C"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22633">
        <property type="entry name" value="F5_F8_type_C_2"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13385">
        <property type="entry name" value="Laminin_G_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13330">
        <property type="entry name" value="Mucin2_WxxW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02140">
        <property type="entry name" value="SUEL_Lectin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00090">
        <property type="entry name" value="TSP_1"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00231">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00607">
        <property type="entry name" value="FTP"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="PAN_AP"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="6"/>
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    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="10"/>
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        <property type="entry name" value="Hairpin loop containing domain-like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="15"/>
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        <property type="entry name" value="FA58C_2"/>
        <property type="match status" value="2"/>
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        <property type="match status" value="8"/>
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        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PAN"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="7"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="521"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="11">
        <location>
            <begin position="723"/>
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    </feature>
    <feature type="domain" description="C-type lectin" evidence="11">
        <location>
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            <end position="1056"/>
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        <location>
            <begin position="1573"/>
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        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="14">
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            <begin position="1765"/>
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    </feature>
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        <location>
            <begin position="2483"/>
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        </location>
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    <feature type="domain" description="F5/8 type C" evidence="10">
        <location>
            <begin position="2756"/>
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    <feature type="domain" description="F5/8 type C" evidence="10">
        <location>
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    </feature>
    <feature type="domain" description="F5/8 type C" evidence="10">
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    <feature type="domain" description="F5/8 type C" evidence="10">
        <location>
            <begin position="3596"/>
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        <location>
            <begin position="3782"/>
            <end position="3901"/>
        </location>
    </feature>
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        <location>
            <begin position="3911"/>
            <end position="4004"/>
        </location>
    </feature>
    <feature type="domain" description="SUEL-type lectin" evidence="12">
        <location>
            <begin position="4012"/>
            <end position="4103"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="11">
        <location>
            <begin position="4126"/>
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    </feature>
    <feature type="domain" description="F5/8 type C" evidence="10">
        <location>
            <begin position="4244"/>
            <end position="4396"/>
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    </feature>
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            <begin position="4403"/>
            <end position="4493"/>
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            <begin position="4507"/>
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    </feature>
    <feature type="domain" description="SUEL-type lectin" evidence="12">
        <location>
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        </location>
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    <feature type="domain" description="SUEL-type lectin" evidence="12">
        <location>
            <begin position="4954"/>
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    </feature>
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        <location>
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    </feature>
    <feature type="domain" description="SUEL-type lectin" evidence="12">
        <location>
            <begin position="5287"/>
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        <location>
            <begin position="5382"/>
            <end position="5479"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
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            <begin position="213"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50022"/>
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        <source>
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        <source>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ8032874.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="5483" mass="609135" checksum="38C5292057242455" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BWH0</accession>
    <name>A0A9Q1BWH0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_24178</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BWH0"/>
    <dbReference type="OrthoDB" id="10511838at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="186"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="66"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="195"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="226"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8033816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1011" mass="115011" checksum="DF7C4E17693A97E5" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BXB8</accession>
    <name>A0A9Q1BXB8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SCAN box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_21340</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BXB8"/>
    <dbReference type="OrthoDB" id="10063366at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF13">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCAN box" evidence="3">
        <location>
            <begin position="235"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="329"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="84"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="329"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="344"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="368"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8034480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="570" mass="64537" checksum="2D9F5789290D6CF0" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BXG0</accession>
    <name>A0A9Q1BXG0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tyr recombinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_21590</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BXG0"/>
    <dbReference type="OrthoDB" id="6082565at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="entry name" value="Intergrase catalytic core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35617:SF3">
        <property type="entry name" value="CORE-BINDING (CB) DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35617">
        <property type="entry name" value="PHAGE_INTEGRASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8034661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="153" mass="17457" checksum="A5207F134D7D5ECB" modified="2023-09-13" version="1">MERTPEQFIFYVNEKLKQSRPGKMGVKLSFPKYAKDELICVYSAIDTYLGITRPFRTDNKFFISYLKPHKAVTKETISRWIKLVLGASGLNTDIFQAHSTRAASASAAFDRNLPIDLILQTAGWSNERTFALFYNKEITKSDCVSQFQAAIMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BXS6</accession>
    <name>A0A9Q1BXS6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Mitochondrial import receptor subunit TOM70</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_21523</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the Tom70 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BXS6"/>
    <dbReference type="OrthoDB" id="66418at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030943">
        <property type="term" value="F:mitochondrion targeting sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045039">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46208">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46208:SF1">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13414">
        <property type="entry name" value="TPR_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13174">
        <property type="entry name" value="TPR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="13" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 10" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="2 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="16"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="10">
        <location>
            <begin position="74"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="10">
        <location>
            <begin position="113"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="10">
        <location>
            <begin position="315"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="10">
        <location>
            <begin position="424"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="10">
        <location>
            <begin position="493"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="40"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="41"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="68"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8034613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="559" mass="62340" checksum="AF43AC311C1E14BF" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BXV8</accession>
    <name>A0A9Q1BXV8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Fibroblast growth factor receptor 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_21877</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BXV8"/>
    <dbReference type="OrthoDB" id="1915767at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005126">
        <property type="term" value="F:cytokine receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004715">
        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007259">
        <property type="term" value="P:cell surface receptor signaling pathway via JAK-STAT"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019221">
        <property type="term" value="P:cytokine-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051286">
        <property type="entry name" value="JAK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45807">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE HOPSCOTCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45807:SF7">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE HOPSCOTCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000615">
        <property type="entry name" value="TyrPK_CSF1-R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="153"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="306"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000615-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8034862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="456" mass="51967" checksum="EE54B9A860FEC5C9" modified="2023-09-13" version="1">MATIILQLSAEQKVAFLGDTANLEFFLGPAASSALITWQKGNEHLLNHDLKPVKTTKGRYSSLLDEILMTVWLEISDITFQDAGNYSCIVYKQFKGTPVENEWTLLVQGMDLHLAISSPERMNLPVVTDRLSSSSSIKQLTEVSTKEILADDLVFVRPLETGRVFKRWAGMLQKYNGSEKHVQILQPTALDTDSLGEWNCHIGGLLKLDEHRNIISTLGFCIVSSTAYCIQDSTSSESVRSYIRRNFNQLSCDTYSIFELPHRLISFAMDIIRGVKFLNCNKWLHPGLCLDKLLLADDGHCLKLYDFCKMSDAVKKMGTYKAKAENILFLAPEVILKNEYTFKSELWAIAITFWEIFSSGQNVPIDPHKTEHMKIHQKLGQPSSCPEPVYEIMKPCWSLDPATRPSLEELEEAFINLQNEYYCIQFDRHDDKFHKDETNLKGECNDYEPCLPVQRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C002</accession>
    <name>A0A9Q1C002_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_19483</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8035720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C002"/>
    <dbReference type="OrthoDB" id="10061868at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005312">
        <property type="entry name" value="DUF1759"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03564">
        <property type="entry name" value="DUF1759"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="55"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8035720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="265" mass="30066" checksum="146412E3E775661E" modified="2023-09-13" version="1">MAECEQDFVQVLLRVRRVVDRQSPLDNCTSNASVPTTPQSNPTTTVPTQPASTGTSPGPVPTVQQPTSQPSLSSAPRMERMKFPKFSGDIKDYKRFKKLFTHCTANLTETECFYQLTESMSHARERNKIKGCINIERAWQVLDDCYGDNDKVVDRLLQDLNNLRPYEHKGKTNLQEMDRFVQTLQTFETQAEGIGLSGELNSKIMLSQIKQKLPEEHHIAYYKSVRDDSTDDTLAGLVKWLHRQLLLLEKAKPFAADNPPRSPQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C0C3</accession>
    <name>A0A9Q1C0C3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Monocarboxylate transporter 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_19896</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C0C3"/>
    <dbReference type="OrthoDB" id="2213137at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008028">
        <property type="term" value="F:monocarboxylic acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF303">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="144"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="179"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="206"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="276"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="312"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="341"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="367"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="401"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="427"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8036037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="462" mass="51004" checksum="9FDC4DE32D18124B" modified="2023-09-13" version="1">MFYARCGRQLYKTVLLMSYCYQLAQTVNEQFVGRLSMSRRHLATMGYNENYQYFSAVVSRCIFEFLLGGSMKAYGLMTTFVTEKLAISHSVLGLILCLQQSVGYLTSPVISQLVTYYGIRPVALLGGLLFGLGYFGQSFFHNHVVFFIIFCTCSGFGTGCLTLSSYFHMLQVFGDGFGLATSITSQCLFLGVCLIPLIFEHLWDQFGLHGALLIFSAFCWNCIISAVLMKPTQTKLSETVTECSKEDEEANSNSIFIKLKQLFLNMKQLVNKHKTIPLLLVIVYVHFACYFSWALFLVPVAEQRGFDKSSAVLLSTAGGVGGILGRTGCSILFALNNTQPFFHFFLPLVLQTIAFVGVAYSKTMMSLLVFSGLSGFTLGFIDGSLPGFIPLNLSKAEIPVAFTIHFLVYAVGMESGSYLAGLMFDIFASYTAVFVLEAVFSVIAALSAVIWQLSFISRGVTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C2Y9</accession>
    <name>A0A9Q1C2Y9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Exocyst complex component 7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Exocyst complex component Exo70</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_17891</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the EXO70 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C2Y9"/>
    <dbReference type="OrthoDB" id="1922221at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.170">
        <property type="entry name" value="Exocyst complex component Exo70"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016159">
        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004140">
        <property type="entry name" value="Exo70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046364">
        <property type="entry name" value="Exo70_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12542:SF41">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12542">
        <property type="entry name" value="EXOCYST COMPLEX PROTEIN EXO70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03081">
        <property type="entry name" value="Exo70_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20669">
        <property type="entry name" value="Exo70_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex subunit Exo70 C-terminal" evidence="7">
        <location>
            <begin position="308"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="264"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00026169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU365026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8037144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="680" mass="78103" checksum="9A796B7818378758" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C490</accession>
    <name>A0A9Q1C490_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Major facilitator superfamily domain-containing protein 6-B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_18694</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. MFSD6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C490"/>
    <dbReference type="OrthoDB" id="515887at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024989">
        <property type="entry name" value="MFS_assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051717">
        <property type="entry name" value="MFS_MFSD6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16172">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 6-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16172:SF41">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 6-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12832">
        <property type="entry name" value="MFS_1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="119"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="164"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="204"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="246"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="277"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="310"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="245"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8037789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="384" mass="42224" checksum="7D37F2737F0525FD" modified="2023-09-13" version="1">MVDIGLNPFQVGILKCLEPVTIFTTSPIWGSIADKFNVRKGLLILCALGSALCFPLVILVPPVNLLETDEVENTSMRDMQVRNFSDVYGPSGQYLNVSENPGLVNREEWKPSIFTQASLITFTLIGILTLIDNIFVAGFLPLLDSSTMELCRRYPGTSYGGQRWLGAFAVVVFSPIAGFLFDWFENSDINLGDTYFLHSKYLPSFVLFTILMLVSVIPVCLMEVLPSKASPSISRELLVLFKDLHIVLTFVLFIVVGMCKGIVVAFLFIYLEELKASSMLMSLSLVITCLAETPCLIASGKVIEKLGYEVVFASGLFAYIVRLLGYSLLENPWMVLLIEPLHGVCYGLIWPCCTEYANHIAPEGMSSTLQSLAHAAKAGVGTIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C4P3</accession>
    <name>A0A9Q1C4P3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">N-glycosylase/DNA lyase</fullName>
            <ecNumber evidence="3">4.2.99.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HOLleu_16857</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Nanhai2018</strain>
            <tissue evidence="16">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">DNA repair enzyme that incises DNA at 8-oxoG residues. Excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine (FAPY) from damaged DNA. Has a beta-lyase activity that nicks DNA 3' to the lesion.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3-dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-phospho-2'-deoxyribonucleoside-DNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66592"/>
            <dbReference type="Rhea" id="RHEA-COMP:13180"/>
            <dbReference type="Rhea" id="RHEA-COMP:16897"/>
            <dbReference type="Rhea" id="RHEA-COMP:17067"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136412"/>
            <dbReference type="ChEBI" id="CHEBI:157695"/>
            <dbReference type="ChEBI" id="CHEBI:167181"/>
            <dbReference type="EC" id="4.2.99.18"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the type-1 OGG1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.99.18" evidence="3"/>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C4P3"/>
    <dbReference type="OrthoDB" id="238681at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034039">
        <property type="term" value="F:8-oxo-7,8-dihydroguanine DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140078">
        <property type="term" value="F:class I DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006285">
        <property type="term" value="P:base-excision repair, AP site formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.30:FF:000006">
        <property type="entry name" value="N-glycosylase/DNA lyase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1670.10:FF:000005">
        <property type="entry name" value="N-glycosylase/DNA lyase OGG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="entry name" value="Helix-hairpin-Helix base-excision DNA repair enzymes (C-terminal)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.30">
        <property type="entry name" value="Hypothetical protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012904">
        <property type="entry name" value="OGG_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052054">
        <property type="entry name" value="Oxidative_DNA_repair_enzyme"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10242">
        <property type="entry name" value="8-OXOGUANINE DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10242:SF2">
        <property type="entry name" value="N-GLYCOSYLASE_DNA LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07934">
        <property type="entry name" value="OGG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="DNA-glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55945">
        <property type="entry name" value="TATA-box binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-0234">DNA repair</keyword>
    <keyword evidence="10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 16" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HhH-GPD" evidence="15">
        <location>
            <begin position="83"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="265"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="265"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="280"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00044632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00073127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ8039213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="299" mass="34112" checksum="78490EB75151341D" modified="2023-09-13" version="1">MEKNSKKRRKKESSSVKMAPSCLAEVADPERILRDYSQLETQLAPLYEKWKSVDKNFSDRGSLFPGVRILRQDPVENLFSFICSSNNNISRISGMVEKLCTEYGTLAGILDGDKWYNFPSVSSLAGKDTESELRKLGFGYRAKFIHQTANILSEERSTDWLHTLRKLSYEEAHAELQKLPGVGAKVSDCVCLFSLDKPGAIPVDTHVWQIASRDYMPELKSKKSLTDKTYREIGEFFRELFGEYAGWAHSVLFSADLKKFQDIAEGRKSQHSTDEEVVHPNKKQRKSSKKPSKKETKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CAT1</accession>
    <name>A0A9Q1CAT1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_13292</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8042273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CAT1"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8042273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="72" mass="8416" checksum="4042362ACD186F7D" modified="2023-09-13" version="1">MAYCETKFYLQLTASKTQTAAAMWQKPKRAAPKAYYDASRANSLVTYHFNRNLAKEEVKKKYNVSLFMEKIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CAU6</accession>
    <name>A0A9Q1CAU6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GIY-YIG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_12806</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CAU6"/>
    <dbReference type="OrthoDB" id="6782675at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="CDD" id="cd10442">
        <property type="entry name" value="GIY-YIG_PLEs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035901">
        <property type="entry name" value="GIY-YIG_endonuc_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82771">
        <property type="entry name" value="GIY-YIG endonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8041882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="139" mass="15771" checksum="A6B74626455693CB" modified="2023-09-13" version="1">MLSIVGPNNNRFNIHQHFTCTSANVVYVRVCKRCNILYVGETKRRLADRVNEHLRSIKQNLPGFPVATHFNPPSTCSIRDWMVSVAISCRGSDHDRLAAENRLIMKLGTLSPHGLNVRLELLQLSNSCIVFTCLYLLFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CAV7</accession>
    <name>A0A9Q1CAV7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_12823</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CAV7"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="155"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="192"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="156"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8041897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="326" mass="35408" checksum="5F13934AB016B38F" modified="2023-09-13" version="1">MKPQNYHLLIKQKKIPQRFRKLLRCQHKQKQHMVCSASRIIEQTTTIDLPSWEETTTCDSSTHLREITTNTMSTYEETTGEQTTAIEQTTPVGNTNGDTSTHLRKVIPNTMSTLEKVTEVTEQTTAIEKFLTVGTTPGDTSTQATSIRISTYGKATEQTTSIQQSPHVGTTPDDTSTHLGEAISNTMSTYGEATEQTTSIQQSPPVGTTTGDTSTHLGEATKITMSTYGKGREQTTAIVQFLPIGTTPGDTSTRQLPALTHLGEATTNAMSTHRKSTDFRKTITIQQSVSTGTIPGDTSTHLPEASTNVMSTYGDTTGLLPLTSFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CCM3</accession>
    <name>A0A9Q1CCM3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_13540</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8042475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CCM3"/>
    <dbReference type="OrthoDB" id="5982392at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46704">
        <property type="entry name" value="CXC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46704:SF1">
        <property type="entry name" value="TELOMERE LENGTH REGULATION PROTEIN TEL2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8042475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="144" mass="16319" checksum="1A05F2926DABBBF3" modified="2023-09-13" version="1">MMHILHHRHMHRGRSSVHYLPMIDMYPGDKTCILSTLEFISNLATKHHVPPVVTFDQPLYWKAAAEIISASPENSRLKEFILILGTFYTLMNLLGATDTLMNGTGPKNILEVVYGETAVVYTMTVNLVKEQSVVIGWWTSVFTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CCR4</accession>
    <name>A0A9Q1CCR4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_09784</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8042902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CCR4"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8042902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="57" mass="6418" checksum="B54AC4199421E1F1" modified="2023-09-13" version="1">MANKSPISPSSQPLRRTAVPTKSGPTTHFSLEPIKRTKTRKRYQSQGLSRRLHTVCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CEN7</accession>
    <name>A0A9Q1CEN7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_11261</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CEN7"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040498301" evidence="1">
        <location>
            <begin position="25"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8043937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="310" mass="35158" checksum="9A77CC832B7501D9" modified="2023-09-13" version="1">MMTRFLIHILLFKVGTFCLDSASAVSVDGVATGEWDGIDFYISVKRDAIQKKLDAFHISRSNGCDDRPRLYVPDGPILPDLHNSDRHPVYIDFGHMKSHDFDGYLEENPNTCVSVSGLEVAVTIPLLAGAPDGPPVYALALAKFYEKSRGDMPKINFLYPYIPVDEIKMSDYGAVVRNGNDEMRVTFNRLIHPCKPLDSMVNEEFEEFIHSDFQFGLPQPDYSFCDRHSFESKFCANAERIKCGVQRYSPNLCQQWSSKTTKHCVTTVEIKKITSWFREYILLGDDPVNILASETHSSNFSIALKYPCQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CFQ4</accession>
    <name>A0A9Q1CFQ4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_11037</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CFQ4"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8043779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="84" mass="9462" checksum="593D81F4826ED078" modified="2023-09-13" version="1">MCRKLMAVKLLDGQGRFLRHNGRIQRSEKNYPVTMATVDSFQNCICGQLYRGCIIDIDGNLINQKFIGISEKGLPVSLTSDEQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CGE9</accession>
    <name>A0A9Q1CGE9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tRNA 4-demethylwyosine synthase (AdoMet-dependent)</fullName>
            <ecNumber evidence="3">4.1.3.44</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_11349</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Probable component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. Catalyzes the condensation of N-methylguanine with 2 carbon atoms from pyruvate to form the tricyclic 4-demethylwyosine, an intermediate in wybutosine biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">tRNA modification; wybutosine-tRNA(Phe) biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TYW1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.44" evidence="3"/>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8044014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CGE9"/>
    <dbReference type="OrthoDB" id="271553at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102521">
        <property type="term" value="F:tRNA-4-demethylwyosine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031591">
        <property type="term" value="P:wybutosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001094">
        <property type="entry name" value="Flavdoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034556">
        <property type="entry name" value="tRNA_wybutosine-synthase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13930">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT TRNA 4-DEMETHYLWYOSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13930:SF0">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT TRNA 4-DEMETHYLWYOSINE SYNTHASE TYW1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00258">
        <property type="entry name" value="Flavodoxin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00369">
        <property type="entry name" value="FLAVODOXIN"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="10">
        <location>
            <begin position="1"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="186"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="186"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8044014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="398" mass="45265" checksum="1870A7EF134E89CE" modified="2023-09-13" version="1">MFGYFMVLKQKLASDFKEKLAGARLGSKVINLKVFDPEQHILPDIVKNNTLIYIMSTYTDGQPPDSAAWFCNWLEESVNDFRVPRTLLSGVHYSVFGLGNSLYKENYNKVSRNVDIWLKTLHATPVVARGYGDANVANSKHGGIEKDFEAWTNNVLTALRKHLSGDDKKANGFTAKIKEAEAVPEMDIYEDEDDDNTSAGQKEESETPKEMVTPIIRQSLTKQGYKLIGSHSGVKLCRWTKDSFLEEASFILLLQNYYIFSQSMLRGRGGCYKHTFYGIESHRCMETTPSLACANKCVFCWRHHSNPVGTEWKWQMDEPDVIVKGAMENHYKLINQFKGVPGVKEERMQEAMKIKHCALSLVGEPIMYPEINTLVRMLHSHEISTFLVTNAQFPDAIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CHM4</accession>
    <name>A0A9Q1CHM4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_07451</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CHM4"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040457690" evidence="1">
        <location>
            <begin position="25"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8044659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="57" mass="6401" checksum="56DB7697E07F01CE" modified="2023-09-13" version="1">MSQRLLSSIIHRLALSLFARGTSSYATARYRSTTVPSSTCMHYQPLDQTRQILSKGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CI63</accession>
    <name>A0A9Q1CI63_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Protein transport protein SEC23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HOLleu_07980</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Nanhai2018</strain>
            <tissue evidence="18">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Cytoplasmic vesicle</location>
            <location evidence="12">COPII-coated vesicle membrane</location>
            <topology evidence="12">Peripheral membrane protein</topology>
            <orientation evidence="12">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Endoplasmic reticulum membrane</location>
            <topology evidence="12">Peripheral membrane protein</topology>
            <orientation evidence="12">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the SEC23/SEC24 family. SEC23 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CI63"/>
    <dbReference type="OrthoDB" id="10256289at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030127">
        <property type="term" value="C:COPII vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090110">
        <property type="term" value="P:COPII-coated vesicle cargo loading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01478">
        <property type="entry name" value="Sec23-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11287">
        <property type="entry name" value="Sec23_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.730:FF:000005">
        <property type="entry name" value="Protein transport protein SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.380:FF:000001">
        <property type="entry name" value="Protein transport protein SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1670:FF:000006">
        <property type="entry name" value="Protein transport protein SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000003">
        <property type="entry name" value="Protein transport protein SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000011">
        <property type="entry name" value="Protein transport protein SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1670">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.730">
        <property type="entry name" value="Sec23/Sec24 helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.380">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012990">
        <property type="entry name" value="Beta-sandwich_Sec23_24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007123">
        <property type="entry name" value="Gelsolin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036180">
        <property type="entry name" value="Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037364">
        <property type="entry name" value="Sec23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006900">
        <property type="entry name" value="Sec23/24_helical_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036175">
        <property type="entry name" value="Sec23/24_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006896">
        <property type="entry name" value="Sec23/24_trunk_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037550">
        <property type="entry name" value="Sec23_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006895">
        <property type="entry name" value="Znf_Sec23_Sec24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036174">
        <property type="entry name" value="Znf_Sec23_Sec24_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11141">
        <property type="entry name" value="PROTEIN TRANSPORT PROTEIN SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11141:SF0">
        <property type="entry name" value="PROTEIN TRANSPORT PROTEIN SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00626">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08033">
        <property type="entry name" value="Sec23_BS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04815">
        <property type="entry name" value="Sec23_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04811">
        <property type="entry name" value="Sec23_trunk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04810">
        <property type="entry name" value="zf-Sec23_Sec24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82754">
        <property type="entry name" value="C-terminal, gelsolin-like domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81811">
        <property type="entry name" value="Helical domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82919">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 12" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6 12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 12" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <keyword evidence="7 12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zinc finger Sec23/Sec24-type" evidence="14">
        <location>
            <begin position="59"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 trunk" evidence="15">
        <location>
            <begin position="127"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 beta-sandwich" evidence="17">
        <location>
            <begin position="404"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 helical" evidence="16">
        <location>
            <begin position="521"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="domain" description="Gelsolin-like" evidence="13">
        <location>
            <begin position="632"/>
            <end position="720"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU365030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF04810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF04811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF04815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF08033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ8045054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="767" mass="86398" checksum="B62811906082B7EC" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CI68</accession>
    <name>A0A9Q1CI68_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_08977</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CI68"/>
    <dbReference type="OrthoDB" id="10514136at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="144"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8045872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="168" mass="19935" checksum="1FE6634292D60D5D" modified="2023-09-13" version="1">MRTRNNSPLALYKCSMAIKRTKYAEVPSQEELETRVVHNWQKTQPWLEWQLPGTTRERNRSVDIVRRVDDGSFKNREEQVSDDDQEKHDKAIKAQISSIRERSRVITLMMEDCAEMNRKRKESRIHRRSEPMVLDIIDQYASQIIMLSIRIIMACAVLTLLKYCFLYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CIN9</accession>
    <name>A0A9Q1CIN9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Multifunctional methyltransferase subunit TRM112-like protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">tRNA methyltransferase 112 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_08439</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRM112 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CIN9"/>
    <dbReference type="OrthoDB" id="2187549at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070476">
        <property type="term" value="P:rRNA (guanine-N7)-methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21089">
        <property type="entry name" value="Trm112-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.10:FF:000015">
        <property type="entry name" value="Multifunctional methyltransferase subunit TRM112-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039127">
        <property type="entry name" value="Trm112"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005651">
        <property type="entry name" value="Trm112-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12773:SF0">
        <property type="entry name" value="MULTIFUNCTIONAL METHYLTRANSFERASE SUBUNIT TRM112-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12773">
        <property type="entry name" value="UPF0315 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03966">
        <property type="entry name" value="Trm112p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158997">
        <property type="entry name" value="Trm112p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="126"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00030516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8045428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="149" mass="16906" checksum="9426CB75AE092670" modified="2023-09-13" version="1">MRVTALRRMKLLTHNMLTSHVKGVKNGYPLKIEVNEMKIREVDFNPEFISRMIPRLEWKALYEAAQSLDVVGNLSPEVVKDYENDEDFLKEAHRVLMEVEVIKGFLICPESGRKFPIENGIPNMLLNEGEVGDAGPSQSGTSQDDDSMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CNP3</accession>
    <name>A0A9Q1CNP3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transposase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_01657</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CNP3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8049082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="113" mass="13015" checksum="984A5E39CA02D4F1" modified="2023-09-13" version="1">MARLVRITSEKKAYVRLLRLQQNMSFRSVASAAGISKSSVWRICNNDSLRNEVKGNRAGRPDIITERQIRGILRRVEKLRGTYGNFTVRTIMRHESLDPRAVSWRTISLSLTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CPH7</accession>
    <name>A0A9Q1CPH7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_00827</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CPH7"/>
    <dbReference type="OrthoDB" id="6118021at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19963">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19963:SF30">
        <property type="entry name" value="ENDONUCLEASE_EXONUCLEASE_PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="294"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="309"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="40"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="309"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="321"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8048488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="334" mass="38159" checksum="6C7A602496F845C0" modified="2023-09-13" version="1">MESTLPQQPDSNWRAKHHQPGGQLPGRSGMFEPRVQKAGGDGKEYQEVPHRRPQKSVRPAIYDGTTPWDNYSTQFELIAELNGWDNYTKAMYLAANLRGEAQGILVDIGAESRRDYYALVTALRARFSPTNQHEVFRIQLKNRQRQKGETLPELGQIIKRLTRQAYPTASFDVLTALATDHFMDALDDPNLRLGVYQARPTNLDEAIRAALEIEAFHMAERQRSTLGRKMARVVQGNLTDQTTKPEVVEEKTLREMILSLIQQMKDLQSKVEKPPTYPRSGKRLRPIDMTTVQCYQCKEYGHYKRNCPQKKETTLDDSGSRKPTQIPNEAMSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CPU2</accession>
    <name>A0A9Q1CPU2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_01349</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CPU2"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="1">
        <location>
            <begin position="319"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8048865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="457" mass="51404" checksum="7035575760DE97AB" modified="2023-09-13" version="1">MGRMGIVQCNFEEGFYGVVWYNLEEFSKGHSFLQLINGVKKGEGYLSGDFDVLPNGSLMILNVSLQHDSNFVVSKFRSEDELPLSFDVRVVVTVKPSTAFPVISFCGEKSNTCFVLLDEYSELVCSVKLARPAIDLQWAVRGVHGDDESVFQSSVTDIGHVFTSRVTMINVFPHSRVLHLLVCKADNHYGLLLEEESTIFIEKSDISLGALETVSRFIERDSRLELNCLDKYTKFIVWKKANHRDDTFQVLSYAALFTGNVLNVYSNDFELQKNGSLVLPRTETRHEALYGCISQSENSSKAALFNVVVFVHLLVLAYPVISGCTQYQYCFLEAPAEGSLKCAVRGVRPRVKLEWKTLAKEDSALIIFTNHEVTVNSIGDVYDVILTSTYRVEDTATDRLTLECRMTSSNISELTSAAKFDLFITNGKIKFRIQNVLEHEPFVFESCLYASCTSTDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CQY0</accession>
    <name>A0A9Q1CQY0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sulfotransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_01815</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CQY0"/>
    <dbReference type="OrthoDB" id="10010208at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053259">
        <property type="entry name" value="Golvesin-related_Golgi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005331">
        <property type="entry name" value="Sulfotransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32301">
        <property type="entry name" value="COUNTIN RECEPTOR CNR3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32301:SF6">
        <property type="entry name" value="GOLVESIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03567">
        <property type="entry name" value="Sulfotransfer_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8049190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="253" mass="29730" checksum="36BF43076BD939A7" modified="2023-09-13" version="1">MPRTAQGGSSCKRKQEILQNANYYNSSKERPVVRGHGSLGLCDFIKEKITDRKCSVFTVFRDPYDRLVSHYFFNRQVEKRKASGDQSDLTLNKSIKEWIKGSGSVVWRAFSNEWKFEKMNHGKEKCKELQHFVTREERWPSIDDHLVDIANNLDKHLSFIGLTEDLQTTYQMLEEVYGLPFVEVCSDMHVLQGSYELTNATEQRALKMSAKREIMEDPEIQRLIQFEVLMYEKAKEIFEAQKKVFLKLKESRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CR65</accession>
    <name>A0A9Q1CR65_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Zinc finger imprinted 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_03776</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CR65"/>
    <dbReference type="OrthoDB" id="6077919at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000383">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000358">
        <property type="entry name" value="zinc finger protein 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000690">
        <property type="entry name" value="Zinc finger protein 354C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000110">
        <property type="entry name" value="Zinc finger protein-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006612">
        <property type="entry name" value="THAP_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226:SF416">
        <property type="entry name" value="FI01424P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226">
        <property type="entry name" value="ZINC FINGER AND SCAN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00692">
        <property type="entry name" value="DM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00980">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50950">
        <property type="entry name" value="ZF_THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="THAP-type" evidence="12">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="414"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="442"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="470"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="498"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="526"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="554"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="593"/>
            <end position="616"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8050537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="625" mass="71068" checksum="9BAC3719A9A020FF" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CRZ8</accession>
    <name>A0A9Q1CRZ8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP-dependent DNA helicase</fullName>
            <ecNumber evidence="1">5.6.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_03467</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.3" evidence="1"/>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CRZ8"/>
    <dbReference type="OrthoDB" id="416437at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010285">
        <property type="entry name" value="DNA_helicase_pif1-like_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051055">
        <property type="entry name" value="PIF1_helicase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47642">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47642:SF5">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05970">
        <property type="entry name" value="PIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0227">DNA damage</keyword>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="1" id="KW-0234">DNA repair</keyword>
    <keyword evidence="1 3" id="KW-0347">Helicase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA helicase Pif1-like DEAD-box helicase" evidence="2">
        <location>
            <begin position="315"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8050313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="787" mass="89880" checksum="67074E8AD268CBDC" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CSH3</accession>
    <name>A0A9Q1CSH3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_03135</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CSH3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8050078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="67" mass="8084" checksum="1B031FB96574E153" modified="2023-09-13" version="1">MPTPPHPFCADITFYHGAPRHFSKPAEPTLSRHLHLYRHKDNPRQKWWNKISYEYYVAVLRLVKLVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CTE6</accession>
    <name>A0A9Q1CTE6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP-dependent DNA helicase</fullName>
            <ecNumber evidence="1">5.6.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_03887</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.3" evidence="1"/>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CTE6"/>
    <dbReference type="OrthoDB" id="416437at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010285">
        <property type="entry name" value="DNA_helicase_pif1-like_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051055">
        <property type="entry name" value="PIF1_helicase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47642">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47642:SF8">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05970">
        <property type="entry name" value="PIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0227">DNA damage</keyword>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="1" id="KW-0234">DNA repair</keyword>
    <keyword evidence="1 3" id="KW-0347">Helicase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA helicase Pif1-like DEAD-box helicase" evidence="2">
        <location>
            <begin position="2"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8050618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="527" mass="59319" checksum="CB8EFBEA5DD3087F" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CTG8</accession>
    <name>A0A9Q1CTG8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_04133</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CTG8"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="35"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8050796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="52" mass="5338" checksum="C23DD5DD64598599" modified="2023-09-13" version="1">MPNSLSGLKATEQAMASCLRVGTSQSLVTTILPRGSSGSSTNGALSSHIYLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1GZ54</accession>
    <name>A0A9Q1GZ54_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="16">PC3-like endoprotease variant A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HOLleu_32633</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Nanhai2018</strain>
            <tissue evidence="16">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S8 family. Furin subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1GZ54"/>
    <dbReference type="OrthoDB" id="300641at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04059">
        <property type="entry name" value="Peptidases_S8_Protein_convertases_Kexins_Furin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000006">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.850">
        <property type="entry name" value="Peptidase S8, pro-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034182">
        <property type="entry name" value="Kexin/furin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002884">
        <property type="entry name" value="P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032815">
        <property type="entry name" value="S8_pro-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038466">
        <property type="entry name" value="S8_pro-domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42884:SF23">
        <property type="entry name" value="FURIN-LIKE PROTEASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42884">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01483">
        <property type="entry name" value="P_proprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16470">
        <property type="entry name" value="S8_pro-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51829">
        <property type="entry name" value="P_HOMO_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 11 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 11 12" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11 12" id="KW-0720">Serine protease</keyword>
    <keyword evidence="4 14" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040444849" evidence="14">
        <location>
            <begin position="23"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="domain" description="P/Homo B" evidence="15">
        <location>
            <begin position="542"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="197"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="810"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="201"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="820"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="10 11">
        <location>
            <position position="174"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="10 11">
        <location>
            <position position="222"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="10 11">
        <location>
            <position position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ8027480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="860" mass="95946" checksum="C993F115D61D1F90" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1GZY4</accession>
    <name>A0A9Q1GZY4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Major facilitator superfamily domain-containing protein 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_30008</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. MFSD6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8027915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1GZY4"/>
    <dbReference type="OrthoDB" id="515887at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024989">
        <property type="entry name" value="MFS_assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051717">
        <property type="entry name" value="MFS_MFSD6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16172:SF42">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16172">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 6-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12832">
        <property type="entry name" value="MFS_1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="72"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="257"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="301"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="330"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="370"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="401"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="434"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="459"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="493"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="528"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="369"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="565"/>
            <end position="597"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8027915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="695" mass="77199" checksum="5C93DECFABE30623" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H0P6</accession>
    <name>A0A9Q1H0P6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nascent polypeptide-associated complex subunit alpha-like UBA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_30645</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H0P6"/>
    <dbReference type="OrthoDB" id="3169036at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8028425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="148" mass="16582" checksum="B2EFC93A6C00F060" modified="2023-09-13" version="1">MDGFALPLHRLDWTKHRLECYPVDNANKPDDEKGKADDAERYLSDTCDVSLGANVDLDDSERKKIRVPAEKLKLIHKGRKINKDNILEVVKERAVFQAIGVEALSEEGLNSESVSYIMGQMNVDRNSAITALRLKGDVIDAILYLGNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H1G3</accession>
    <name>A0A9Q1H1G3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Mitochondrial import inner membrane translocase subunit tim16-B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_30815</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TIM16/PAM16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H1G3"/>
    <dbReference type="OrthoDB" id="10262892at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005744">
        <property type="term" value="C:TIM23 mitochondrial import inner membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000006">
        <property type="entry name" value="Import inner membrane translocase subunit TIM16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005341">
        <property type="entry name" value="Tim16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12388">
        <property type="entry name" value="MITOCHONDRIA ASSOCIATED GRANULOCYTE MACROPHAGE CSF SIGNALING MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12388:SF0">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03656">
        <property type="entry name" value="Pam16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0811">Translocation</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="116"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8028546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="138" mass="14871" checksum="8ECB06505F445AEB" modified="2023-09-13" version="1">MAAKYLAQIIILGGQVVGRAFAKAVRQEIQASQQAAKQRGGGQAGAKTAAANSLAGMTLEEAQKILNLSKLDAAEIEKSYQHLFEVNDKAKGGSFYLQSKVVRAKERIDSELKMAQEAEGRKLASETQKTETKDTSQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H1K4</accession>
    <name>A0A9Q1H1K4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">GSK3B-interacting protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_24987</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GSKIP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H1K4"/>
    <dbReference type="OrthoDB" id="5804279at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019207">
        <property type="term" value="F:kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051018">
        <property type="term" value="F:protein kinase A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060828">
        <property type="term" value="P:regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2280.10">
        <property type="entry name" value="Hypothetical protein (hspc210)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037395">
        <property type="entry name" value="GSKIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007967">
        <property type="entry name" value="GSKIP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023231">
        <property type="entry name" value="GSKIP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12490">
        <property type="entry name" value="GSK3B-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12490:SF4">
        <property type="entry name" value="GSK3B-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05303">
        <property type="entry name" value="GSKIP_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103107">
        <property type="entry name" value="Hypothetical protein c14orf129, hspc210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GSKIP" evidence="2">
        <location>
            <begin position="19"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8031707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="140" mass="15591" checksum="63F1329D17DF09E7" modified="2023-09-13" version="1">MADSADSQTPVEGDIKLMKIEAEAAIREVKYAVEHAEISSELSANDDIAFFNVRTKEGETFCVELSALGYRVIVGNKFDEISSEMNSTYYETIYSLLDKHSPKYRLTFGEALAGKLEGLQKLQEKAANGADHMETEEQKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H205</accession>
    <name>A0A9Q1H205_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_28511</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H205"/>
    <dbReference type="OrthoDB" id="6783964at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33480">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33480:SF1">
        <property type="entry name" value="TYR RECOMBINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8029176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="186" mass="20372" checksum="627358F3B6793251" modified="2023-09-13" version="1">MLASLKRTNIFFGSFSALTPSTACDSLRIKTDEANLENPELIKSTKLRKYTATLSQVSNLQDNHYQWLADHLGHTCKTFKAMIKKGSLLGKEAKIAKLLLATDAGEASSCMGKTLDEISFEEIPSAFSEVNEPVASNTAQSTESKKQSKKQHQSTSGGTGIKETPKHGQRKVKAKAWCPEDNQILI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H254</accession>
    <name>A0A9Q1H254_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Polyubiquitin-C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_28729</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H254"/>
    <dbReference type="OrthoDB" id="428577at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="CDD" id="cd17039">
        <property type="entry name" value="Ubl_ubiquitin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019956">
        <property type="entry name" value="Ubiquitin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050158">
        <property type="entry name" value="Ubiquitin_ubiquitin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10666">
        <property type="entry name" value="UBIQUITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00348">
        <property type="entry name" value="UBIQUITIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="1">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="1">
        <location>
            <begin position="62"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="1">
        <location>
            <begin position="141"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="1">
        <location>
            <begin position="218"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="1">
        <location>
            <begin position="296"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8029356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="428" mass="48406" checksum="FA33C1D7CC42DAC8" modified="2023-09-13" version="1">MDTIEHLKLLLQDREAVEVDVQRLIFAGKQLEDGRTLSDYDIKNEEKIHTVLRLCGGGGGSMPIFVKTLTGKCTQLILGPSDTIKAVKSQIQGMEDIRSDEQLLLNGGKILEDGKNLSDYNIGEGSTIILVLRSRCDIDVVKIFIEGMAGKIITLHCTVNDTIEQIKEYIQKYIGDAPKERQRLFYKGKELEDEKNCPFYNIVKNSTVILQMRPQNGMQIKVSMPNITTDRPFLLEVLRDETIAANKSKIIPLLGGSQEYVFIAFEGKLIQDESKSLQDYNIHHKSTICVVYSFHYEMKVQVMKSFGPEQHEMIVKVKSNDTVKKLKWKIQGSEGIPPDQQLLIFKGQKLNDDNTLYDYGIDENSCIHACKSTGNKTTYVVNYSINGEDICFEVSGAMTIESILTTIHARNENSFYSLPYIKLGVKHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H282</accession>
    <name>A0A9Q1H282_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GIY-YIG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_26753</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H282"/>
    <dbReference type="OrthoDB" id="10025388at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="CDD" id="cd10442">
        <property type="entry name" value="GIY-YIG_PLEs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000305">
        <property type="entry name" value="GIY-YIG_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035901">
        <property type="entry name" value="GIY-YIG_endonuc_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82771">
        <property type="entry name" value="GIY-YIG endonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50164">
        <property type="entry name" value="GIY_YIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GIY-YIG" evidence="1">
        <location>
            <begin position="161"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8030358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="261" mass="29141" checksum="048D8C82DFB33994" modified="2023-09-13" version="1">MVSFGGKGRGSINSLYAFQGIREHSLWALKRIQGIDRASALAPKTDHTPTRRIPLVLSFHPSVTPIVRAIYRNVETLRHDPSTRDHFPDPPITAFRIEKNISKHLVRASQPQAVVPDTPGTFPCNRGRCNTCPVVSYDKNLSIVGPNNNRFNVHQHFTCTSANVVYVLVCKRCNILYVGETKRRLADRVTEHLRSIKQNLPGFPVATHFNPPSTCSIRDLMVSAAISCRGSDHDRLAAENRLIMKLGTLSPHGLNVRLELL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H3E7</accession>
    <name>A0A9Q1H3E7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_24236</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H3E7"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8033867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="157" mass="17510" checksum="4FF6C7CC944339D8" modified="2023-09-13" version="1">MKDRETKMLILAVLVGYFAIGTTVSAEQLCHLVSFIHDGRMVWALKSDNILECTQIAIGGHSSLELLPEKLILFESPGILLTAGVNVQAILNNNRMYTFFNYKSGRHHSLSIENGPFECTQENVSPVTAVDSTTNLSVEGELYKLSLPLIKEYEFFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H3F3</accession>
    <name>A0A9Q1H3F3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Carbohydrate sulfotransferase 15</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_27706</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8031095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H3F3"/>
    <dbReference type="OrthoDB" id="8068875at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050659">
        <property type="term" value="F:N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019319">
        <property type="term" value="P:hexose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052654">
        <property type="entry name" value="CS_Sulfotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15723">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15723:SF0">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="173"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="189"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8031095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="602" mass="70166" checksum="6C320B86A933B490" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H3J7</accession>
    <name>A0A9Q1H3J7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_24866</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H3J7"/>
    <dbReference type="OrthoDB" id="10626906at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C-type lectin" evidence="2">
        <location>
            <begin position="221"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="171"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8031618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="350" mass="38781" checksum="CAF0A71DA874A28E" modified="2023-09-13" version="1">MGNKSTGRDDCYGSASTLEGKTVKIDCSLLNGVVCIQISGDVKSSEEAKQDLYLNMDDIPRDRDIQQAQLISEFYVDSQQFDDLVLSSMMKKRKKRRAPYNGRRILTGAHRSKIQTIRTKTGKQPLQSRTFNGRIGNTRRVTSTRNNIVTVQEDQGAPADQKPLDVQVPAVGESPAQKTPDREKKPILGENRGLFKGGQKQEVPDNSRTSKKRGKQGREMCMPGWSSIAGSCIQYFDISLTWEEAKATCEGFSYKESKATLITLEEEGMRTRVLALLDRIAARTGKAIGSFWTSGTNLQEAGTDADPLAADRNCVAHMGTDGLQPELCSQRYSYVCQLKAPIISEDDLVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H3V3</accession>
    <name>A0A9Q1H3V3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_25240</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H3V3"/>
    <dbReference type="OrthoDB" id="6283219at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005312">
        <property type="entry name" value="DUF1759"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03564">
        <property type="entry name" value="DUF1759"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8031888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="148" mass="16706" checksum="AF6EFDAE5298CEEB" modified="2023-09-13" version="1">MQTLNMSINMPKPDLQVFRGNPGIYYSFINNFEARVATKLTDDRQKLTYLLQVCGGDAKDSIACCVLMDPRLGYHRAKEILAQQFGQPHVVAHSLIDKVVNRPAIKPNDYKVLAELARQIRRCEVTLSHSGYTADLDSTDTLLKIQQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H8L9</accession>
    <name>A0A9Q1H8L9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Deleted in malignant brain tumors 1 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_20295</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H8L9"/>
    <dbReference type="OrthoDB" id="536948at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000001">
        <property type="entry name" value="Lysyl oxidase 4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000016">
        <property type="entry name" value="Scavenger receptor cysteine-rich protein type 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19331:SF465">
        <property type="entry name" value="EGG PEPTIDE SPERACT RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19331">
        <property type="entry name" value="SCAVENGER RECEPTOR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00530">
        <property type="entry name" value="SRCR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00258">
        <property type="entry name" value="SPERACTRCPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00420">
        <property type="entry name" value="SRCR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="SRCR" evidence="10">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="10">
        <location>
            <begin position="44"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="10">
        <location>
            <begin position="162"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="10">
        <location>
            <begin position="272"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="6"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="69"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="82"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="113"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="230"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="340"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8036346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="405" mass="44206" checksum="CA1D9B0B958CD3E5" modified="2023-09-13" version="1">MDEVSCVGDEERLDQCSFRGWFLHDCVHQEDAGVRCSSAEDGGIRLIGGINLLEGRVEVFFGGTWGTVCDAGWDINDATVVCRALGFMSADAAVANAYYGNGTGEILLSNVGCTGSEKELNECNFEYAIEHNCSHNNDVGVRCTATEVTTSSEHRVCNNYDVRMVNGPIQSDGQVEVCYKGLWGTVCASSWRREGAFVVCRQLGYDTVGTFYSRVSTEDSSPQIVQDLFCNGNETSLSECAITFGSQENCSFFAALNCSMKVSNPECTDGEVRLAGGLHDDNGTAQICERGHWVNLCASSWEDTESLVICRQLGYTEVDSFNVFDHQGNSKGNITSSISCSGKETVISQCSFSSNEINSCVQIVNVTCEIADSETPDDSKGKFYFLLLIYFSLSLQSRFNCKGFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H944</accession>
    <name>A0A9Q1H944_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_18438</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H944"/>
    <dbReference type="OrthoDB" id="5989905at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8037589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="131" mass="15404" checksum="705FBCD3A5060728" modified="2023-09-13" version="1">MAVLKGQSNFTQNMNENFQKECKEWVRLFTKIYQSKDVTPYMHVLAYHIPEELHIHGNINIYSQQGMEETNDSVTAWFFRATNHQNTTTALQQILATQNRIEYPSFKCHRNKQFSVTCSFCSQKVTTMLML</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H995</accession>
    <name>A0A9Q1H995_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Active regulator of SIRT1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">40S ribosomal protein S19-binding protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_17709</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AROS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H995"/>
    <dbReference type="OrthoDB" id="6493910at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019899">
        <property type="term" value="F:enzyme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023262">
        <property type="entry name" value="AROS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31454">
        <property type="entry name" value="ACTIVE REGULATOR OF SIRT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31454:SF2">
        <property type="entry name" value="ACTIVE REGULATOR OF SIRT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15684">
        <property type="entry name" value="AROS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02029">
        <property type="entry name" value="ACTREGSIRT1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="17"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="50"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00032748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8037006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="172" mass="19940" checksum="15586E38DB3F5BE3" modified="2023-09-13" version="1">MSSSLVRRGLELFQEDVTESGGSSIKRRKKDLSSSNRKDLLPLISSSKTGVKKQLKRLKGNTPRNKTTVKEMKMKSAFDKLSKHKTEDHTDSNVNFLLKMDRSRDRFSSHTNKIIEHCRGKLAKDNPLPETEQEETSVFTEEDFKSFEESYVPKRKFESFLLDYQNFISGKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HCG0</accession>
    <name>A0A9Q1HCG0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">T-box transcription factor TBX3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_11631</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8044232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HCG0"/>
    <dbReference type="OrthoDB" id="7442607at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001708">
        <property type="term" value="P:cell fate specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20188">
        <property type="entry name" value="T-box_TBX2_3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.820:FF:000003">
        <property type="entry name" value="T-box transcription factor TBX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.820">
        <property type="entry name" value="Transcription factor, T-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046360">
        <property type="entry name" value="T-box_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036960">
        <property type="entry name" value="T-box_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001699">
        <property type="entry name" value="TF_T-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018186">
        <property type="entry name" value="TF_T-box_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11267:SF181">
        <property type="entry name" value="OPTOMOTOR-BLIND PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11267">
        <property type="entry name" value="T-BOX PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00907">
        <property type="entry name" value="T-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00937">
        <property type="entry name" value="TBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00425">
        <property type="entry name" value="TBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01283">
        <property type="entry name" value="TBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01264">
        <property type="entry name" value="TBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50252">
        <property type="entry name" value="TBOX_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="T-box" evidence="8">
        <location>
            <begin position="106"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="293"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="628"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="293"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="324"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="336"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="362"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="374"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="388"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="410"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="464"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="641"/>
            <end position="654"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8044232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="672" mass="73542" checksum="29DDE8D74D22E4B9" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HCI9</accession>
    <name>A0A9Q1HCI9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Phosphatidylinositol glycan anchor biosynthesis class U protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_11752</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PIGU family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HCI9"/>
    <dbReference type="OrthoDB" id="549017at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042765">
        <property type="term" value="C:GPI-anchor transamidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016255">
        <property type="term" value="P:attachment of GPI anchor to protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009600">
        <property type="entry name" value="PIG-U"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13121">
        <property type="entry name" value="GPI TRANSAMIDASE COMPONENT PIG-U"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13121:SF0">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL GLYCAN ANCHOR BIOSYNTHESIS CLASS U PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06728">
        <property type="entry name" value="PIG-U"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040184207" evidence="10">
        <location>
            <begin position="30"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="82"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="141"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="181"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="231"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="264"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="291"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="320"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="357"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8041054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="415" mass="47421" checksum="E4BACD376BB0C2AE" modified="2023-09-13" version="1">MAASIVWITLLGILSRVIILGSHFSNVLSDRVELSTPLTSWKRMTEGMFLIKNGISPYLGDNFHEPPLLLYFFTIMDNTTPALIPLVFLFLDLTTAFLLYRVAKYLLRYLLSEQIKEGPSYAKDVDVLLLKRSSLSSIPTLVLSIYLLNPFSILTCSAQSTIIITNVLVTATFLLTLKGQWWSATLSLALASYQSLYPVMLIVPCAMHVANLKMQENKESISYSSPTAVTIMLKIALSFLLWSVILITLSFLMLESWDFLQSTYGFILSVPDLKPNLGLFWYYFMEMFEHFRIFFLWVFQMNAFIYTIPLTIILWKCPSFVFFCQLVLIAIFKSYPSLGDITVYLALLPVWSHTFHYLRNGLVVSVMIVISTVLAPVLWHLWIYAGSANANFFFAFTLIYNTAQVYRHCRAVESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HCY0</accession>
    <name>A0A9Q1HCY0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_14984</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HCY0"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="129"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="22"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="129"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="147"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8040636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="158" mass="18146" checksum="1844EA169B50D6B7" modified="2023-09-13" version="1">MEEKLDIILEQLKKLQSLPSQIEAMQKELKSLKGAVTDMAESIEFIENEADRVKESVKNKAGKEQVDLLKDSLAKMEESMTRKLEDFENRSRRQNLFFGVPELGETQVENQIKKDILETLMGFPSIETERRIHRSIDPPEGRDPSMSDFSATVTEKKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HDA3</accession>
    <name>A0A9Q1HDA3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Carbohydrate sulfotransferase 15</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_12878</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HDA3"/>
    <dbReference type="OrthoDB" id="8068875at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050659">
        <property type="term" value="F:N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019319">
        <property type="term" value="P:hexose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052654">
        <property type="entry name" value="CS_Sulfotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15723">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15723:SF0">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8041939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="434" mass="51227" checksum="EF39BD7452CCED0F" modified="2023-09-13" version="1">MLRHEELCPKTEAMLFFFASSLVFVMCYKLLWNDNHLTFKTPVAHLTSEKLSLRFRPERVVLPSNTSSNPTIPSSDTNSSAVSEKIPVSVWKPYVKELFDRFPENFSSRYLNPCWEEGTTLRCVPYFYQIGSFKCGTTDLWDKIVQHPQVVTVAKEPHWWAWRRFGFKDTPIHRQLVLKIRRITGNEDDHALQWYLNLFSHEATTRIQSNPRLVIGDASISTLWGLGDYEWEQHFPDKRNPPFFLADVLHALQPTAKITVILRDPVEKLWTTYLLDQWKRHLTPKKFHFHWQMLHKESVQCESLNTPTYCAFMHGSTADLSLKNYLYQGIYYLYLKEWVNAYGLENIHVLRLEDWKATPIAELASVIKYLELDPLPMKTLKAITNRATQNVNVRAKKRNITMFTSTRTELQNFYRPWNKKLAELLKDPKYLWDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HEP6</accession>
    <name>A0A9Q1HEP6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_10732</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HEP6"/>
    <dbReference type="OrthoDB" id="5988523at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46579">
        <property type="entry name" value="F5/8 TYPE C DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46579:SF1">
        <property type="entry name" value="F5_8 TYPE C DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8043574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="176" mass="19988" checksum="52EC31777B62FD96" modified="2023-09-13" version="1">MIRELTKAEFFLQYFPCDAVARCLLQNFHQYNGSFGCGTCFHEGETVEKGMGFTRVYPVRGVVECRTAQNTLERAVDAVRLNRVIQGVKGPTILSLLPKFDLIDGFVPEYMHSVILGVVRRFAKLWLDSENHEKPFYIGKFTSTLSNLLQAIKPCSEGDRLPCCISQRKFWKATVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HF96</accession>
    <name>A0A9Q1HF96_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_09841</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8042946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HF96"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8042946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="52" mass="5973" checksum="3AEA0AE58DFE2D33" modified="2023-09-13" version="1">MRCGERKNPIVFGGGQRSSGVTGGQNLKILFTRYLKLESLDRFHAWYVDALW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HGW0</accession>
    <name>A0A9Q1HGW0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycine N-methyltransferase</fullName>
            <ecNumber evidence="3">2.1.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HOLleu_08337</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Nanhai2018</strain>
            <tissue evidence="15">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>glycine + S-adenosyl-L-methionine = sarcosine + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19937"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57433"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="2.1.1.20"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:19938"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the class I-like SAM-binding methyltransferase superfamily. Glycine N-methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.20" evidence="3"/>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HGW0"/>
    <dbReference type="OrthoDB" id="3647at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005542">
        <property type="term" value="F:folic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016594">
        <property type="term" value="F:glycine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017174">
        <property type="term" value="F:glycine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904047">
        <property type="term" value="F:S-adenosyl-L-methionine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051289">
        <property type="term" value="P:protein homotetramerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006111">
        <property type="term" value="P:regulation of gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046498">
        <property type="term" value="P:S-adenosylhomocysteine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046500">
        <property type="term" value="P:S-adenosylmethionine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901052">
        <property type="term" value="P:sarcosine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000113">
        <property type="entry name" value="Glycine N-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.46.10">
        <property type="entry name" value="Glycine N-methyltransferase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014369">
        <property type="entry name" value="Gly/Sar_N_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025714">
        <property type="entry name" value="Methyltranfer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16458">
        <property type="entry name" value="GLYCINE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16458:SF2">
        <property type="entry name" value="GLYCINE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13847">
        <property type="entry name" value="Methyltransf_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000385">
        <property type="entry name" value="Gly_N-mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51600">
        <property type="entry name" value="SAM_GNMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0290">Folate-binding</keyword>
    <keyword evidence="7 12" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12 13" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="8 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase" evidence="14">
        <location>
            <begin position="59"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="117"/>
            <end position="118"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR000385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000385-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF13847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ8045345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="306" mass="35215" checksum="C65D7B885F8063A8" modified="2023-09-13" version="1">MVDSIYRTRSLGVPAQGIPDQYADGKAAKVWEYYIGSRKSRTDVYKKWLTNLLSSKNCDTILDVACGTGVDSVMLLEEGYKVMSIDASDKMVMNAFRERWDRRKEEAFDKWEIELSNWLTVADDIPDRKFDALICLGNSFAHLPDFEGNQANHKIALKNFETLLKPGGILIIDHRNYDAILDTGRAPVKNIYYKGNCIKDIQTSTLFVNGRPSMVTLDYFIDVTAARKMEEEDNQEELPSGRKLKITEDGVYKFRLSYYPHRLNAFTELLKETFGADAKHEVYGDFQPYRENETPAYYVHVIHKPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HH45</accession>
    <name>A0A9Q1HH45_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_08638</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HH45"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8045600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="101" mass="11471" checksum="42716B9EB0B5D912" modified="2023-09-13" version="1">MEVRGSTSMLQLSWTQMFHKEDAASQMLLLGFGVEEMSHRMLNLLTLASVPKRAMFRILQVSESSMISLLMILFALVPRLCFPANKHVDSQNIRLFLSDPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HHJ6</accession>
    <name>A0A9Q1HHJ6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">ATP synthase subunit s, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HOLleu_09212</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Nanhai2018</strain>
            <tissue evidence="15">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the two pore domain potassium channel (TC 1.A.1.8) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8046050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HHJ6"/>
    <dbReference type="OrthoDB" id="5859291at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015271">
        <property type="term" value="F:outward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022841">
        <property type="term" value="F:potassium ion leak channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030322">
        <property type="term" value="P:stabilization of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003280">
        <property type="entry name" value="2pore_dom_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003092">
        <property type="entry name" value="2pore_dom_K_chnl_TASK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11003:SF334">
        <property type="entry name" value="FI03418P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11003">
        <property type="entry name" value="POTASSIUM CHANNEL, SUBFAMILY K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01333">
        <property type="entry name" value="2POREKCHANEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01095">
        <property type="entry name" value="TASKCHANNEL"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0630">Potassium</keyword>
    <keyword evidence="6" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="23"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="105"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="130"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="181"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="217"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="255"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="14">
        <location>
            <begin position="101"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="14">
        <location>
            <begin position="200"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF07885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ8046050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="519" mass="58731" checksum="14390D7D58733971" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HI42</accession>
    <name>A0A9Q1HI42_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Serine/threonine-protein phosphatase 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_08720</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HI42"/>
    <dbReference type="OrthoDB" id="445564at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051134">
        <property type="entry name" value="PPP_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45668">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45668:SF5">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0464">Manganese</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="4">
        <location>
            <begin position="2"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8045673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="221" mass="24964" checksum="19E9F48DDFC89C4B" modified="2023-09-13" version="1">MKLNSYMQNAPCMCYLNHTLPICYTCGNHESETMNQMYGFCGEVKAKYTAQMAELFTEVYNWLPLAHIINNKVLVMHGGLFSSDDVTLDDIRKVDRNRQPPDSGIMCELLWSDPQPQPGRAPSKRGVGIQFGPDITKKFLDRNNLEYIIRSHEVKSDGYEIAHDGKCITVFSAPNYCDTMGNKGALINMSSDLKPKFVTFEAVPHPNVRPMAYANSMFGFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HJJ7</accession>
    <name>A0A9Q1HJJ7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_00263</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HJJ7"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="113"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8048090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="153" mass="16942" checksum="30382072322490E0" modified="2023-09-13" version="1">MGTLASIEQLRYKLVKSSICNAPNNDYVDNSEALNKVIGLSSTEKLSLLHSDNNSSIPNATSEPFIPGNIKIAIGLNTISLITCFFWIIVGLDAMSNVHSHLQSTATVKIPPFCVRINTLNWFIVGVALFHTLFRFVPPFLRLVNLLTTKRIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HK41</accession>
    <name>A0A9Q1HK41_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transposable element P transposase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_00228</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HK41"/>
    <dbReference type="OrthoDB" id="8190203at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon gypsy-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048366">
        <property type="entry name" value="TNP-like_GBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048365">
        <property type="entry name" value="TNP-like_RNaseH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF1">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21788">
        <property type="entry name" value="TNP-like_GBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21787">
        <property type="entry name" value="TNP-like_RNaseH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="284"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="29"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="250"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="299"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="31"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="261"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="320"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8048061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1109" mass="126428" checksum="41B7B8C398AB09AD" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HK71</accession>
    <name>A0A9Q1HK71_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tudor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_03333</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HK71"/>
    <dbReference type="OrthoDB" id="6105938at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="156"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="156"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8050219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="442" mass="50043" checksum="B0AAEDCE338B897C" modified="2023-09-13" version="1">MDLEYNQKKMYVPGTKVQAVDELGRWEEGTVVRYDSSNDSYTVSFNGWSNEFDRDVTSSEIRRPGNVFDQDIASSSTLKRCRKRLRESDYRELLRQLCADDTVTFMKDGQKKTALVSTVDRFRREVTVCLDGGSEQYTIHASFLLNDLLCRPAETTEPRKRQLKKDQPESDGDKRKEGSICINSDQHQQPRCDFYWALCSSGSPIYCGDVVEVSDMDVHLAVHTITDGMKDRKVVGHPVTQEGKINYDTCVSTQAHLIKGKTEIKVSREVSNKLKKIKNNSVVAYISCSKESFNARRNERVLCIKSLALKEMKSLLQGKAPKRLFSWGLCQLDIDPLLFNIGFPGTTRAVFSYSKGNLAKLDTVFGEKWDILVEGTTCSFVTQMVVWITKDNLLNASVQVSKMGLPSHLQRDYRKQLMDILPPSLNGRTFHVPGDDLSAGRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HK85</accession>
    <name>A0A9Q1HK85_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_00293</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HK85"/>
    <dbReference type="OrthoDB" id="10054042at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="52"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="22"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="38"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8048118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="180" mass="20715" checksum="F83FF6133D04243F" modified="2023-09-13" version="1">MATRSGKGYQRTTSLPDAISKETPQPKNKTKANPCTAKFEKLSTRSSRTSEKLKLERQLAIQKVELEFKERELELKLEKLKIQKEMEKTKALLDVVEESVRLESDEDEVDQVITPNILSDMPEDAPNDNVVRFLETMPQSAAEAEDKEEKGTLRELVNSFRLPRIHVEKFKGDPLKYPVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0Y8X7</accession>
    <name>A0A9Q0Y8X7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_44333</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000802">
        <property type="protein sequence ID" value="KAJ8017948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0Y8X7"/>
    <dbReference type="OrthoDB" id="5954038at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="155"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8017948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="249" mass="28795" checksum="69FAF15E550305A1" modified="2023-09-13" version="1">MVSQQTDCQIKFKRILEHFVAANRLKDDECDAIIREYGNFLEGVKASPSAYKEFDPHKDSMRIDTFLFNKMGSNDDYFRLWQRVVCKVLLLSHGQASVERGFSFNKQLEVENLQERSFISQRHVIDHIKSVGGTLSVLVDRKLLMSAAGARQRYLAHLEDEKRKKEKETRVLKRKVADERIKELEKKKARLEDDMKAMQTSADDFAEKAENTGKLTWIAKSNSLRRSAKAKANEVQELVDEIASLKRKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YC87</accession>
    <name>A0A9Q0YC87_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tyr recombinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_42962</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000173">
        <property type="protein sequence ID" value="KAJ8018834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YC87"/>
    <dbReference type="OrthoDB" id="7699712at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="entry name" value="Intergrase catalytic core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35617:SF3">
        <property type="entry name" value="CORE-BINDING (CB) DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35617">
        <property type="entry name" value="PHAGE_INTEGRASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyr recombinase" evidence="2">
        <location>
            <begin position="30"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8018834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="159" mass="17702" checksum="026A21F23979634F" modified="2023-09-13" version="1">MGATKTSKPGQTKICILPFLKVTDICPASVFRHYLERTKSFRSSSNDEDHVFISFLKPYRKVCSATIARWIKDILRLAGVDTGVFTAHSTRGASTSAALRAGMAVEDIMKVADWTRVATFKRFYFRGTTSHTSLAAYVFQVGSLTLKTHLLYLAQCPEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YD79</accession>
    <name>A0A9Q0YD79_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ubiquitin-like modifier-activating enzyme 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_41115</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ubiquitin-activating E1 family. UBA5 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8019494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YD79"/>
    <dbReference type="OrthoDB" id="206053at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071566">
        <property type="term" value="F:UFM1 activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071569">
        <property type="term" value="P:protein ufmylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00757">
        <property type="entry name" value="ThiF_MoeB_HesA_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000066">
        <property type="entry name" value="Putative ubiquitin-like modifier-activating enzyme 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029752">
        <property type="entry name" value="D-isomer_DH_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF9">
        <property type="entry name" value="UBIQUITIN-LIKE MODIFIER-ACTIVATING ENZYME 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00065">
        <property type="entry name" value="D_2_HYDROXYACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="THIF-type NAD/FAD binding fold" evidence="10">
        <location>
            <begin position="52"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="346"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="4"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="368"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8019494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="401" mass="44345" checksum="CD2FFA523B0E92BB" modified="2023-09-13" version="1">MASIQELKAKIKQLEDELALEKRRNAEQVGTPAGSRGKIAKMSAEVVDSNPYSRLMALKRMGIVDNYEKIRDYSVAVVGVGGVGSVTAEMLTRCGIGKLILFDYDKVELANMNRLFFQPHQSGMSKVEAAQKTLSFINPDVIIETHNYNITTMANFDNFMETIRTGSINGDGPVDLVLSCVDNFEARMAINDACNELAQNWIESGVSENAVSGHIQLVKPGETACFGCAPPLVVAENIDEKTLKREGVCAASLPTTMGVVAGLLVQNTLKYLLGFGNVTEYLGYSALQDFFPTMVMKPNSQCSSRYCIKRQEEFQKKLAAQPKEEIQEEVKEEIKHDSNEWAIELVDETSPEESTEKSDENLATGIKYRYDRPDDTKTDDSEVVADTDESMDDLMAKLKGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YED3</accession>
    <name>A0A9Q0YED3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Importin-7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_42958</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000172">
        <property type="protein sequence ID" value="KAJ8018839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YED3"/>
    <dbReference type="OrthoDB" id="10071778at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997:SF18">
        <property type="entry name" value="D-IMPORTIN 7_RANBP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997">
        <property type="entry name" value="IMPORTIN-7, 8, 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8018839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="157" mass="18203" checksum="EA91D4D86431DA6F" modified="2023-09-13" version="1">MEGVIQGVLFPLMCYTDEDDALWTEDPYEYIKLKFDVFEDFISPVTAAQTVLHTSCKKRKEILPKTMAFCMQILQMQDVQPRQKDGAFHMIGTLAEILLKKKMYKDKMELMLVAHVFPEFQNPLGYMRARVCNFKGLACKLHALYCNITRLLGRVIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YGE3</accession>
    <name>A0A9Q0YGE3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_40686</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YGE3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8020951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="124" mass="14204" checksum="782E286BA771A300" modified="2023-09-13" version="1">MPIILYSIEVYFQYPKNLPQKICESLIHAFILSRLDRSNRHMYGLPDTQIFKLQRIQNPAARVLTGTKRPVFKVLHWLPVSTAFLIKLPLLLTNALKVKLCLTCVIEFIAMCQVEILGHSLIGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YJ34</accession>
    <name>A0A9Q0YJ34_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Prostaglandin reductase 1</fullName>
            <ecNumber evidence="4">1.3.1.48</ecNumber>
            <ecNumber evidence="5">1.3.1.74</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">15-oxoprostaglandin 13-reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Dithiolethione-inducible gene 1 protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Leukotriene B4 12-hydroxydehydrogenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">NAD(P)H-dependent alkenal/one oxidoreductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="36" type="ORF">HOLleu_35928</name>
    </gene>
    <organism evidence="36 37">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="36" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="36">Nanhai2018</strain>
            <tissue evidence="36">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="31">
            <text>(5S,12S)-dihydroxy-(6E,10E,12E,14Z)-eicosatetraenoate + NADP(+) = 12-oxo-(5S)-hydroxy-(6E,8E,10E,14Z)-eicosatetraenoate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:51212"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:133974"/>
            <dbReference type="ChEBI" id="CHEBI:133975"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="31">
            <dbReference type="Rhea" id="RHEA:51213"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>13,14-dihydro-15-oxo-PGF2alpha + NADP(+) = 15-oxoprostaglandin F2alpha + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50588"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:133374"/>
            <dbReference type="ChEBI" id="CHEBI:133409"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="27">
            <dbReference type="Rhea" id="RHEA:50590"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="32">
            <text>13,14-dihydro-15-oxo-prostaglandin E1 + NADP(+) = 15-oxoprostaglandin E1 + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50584"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57401"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:133408"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="32">
            <dbReference type="Rhea" id="RHEA:50586"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>13,14-dihydro-15-oxo-prostaglandin F1alpha + NADP(+) = 15-oxoprostaglandin F1alpha + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50592"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:79072"/>
            <dbReference type="ChEBI" id="CHEBI:133411"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="24">
            <dbReference type="Rhea" id="RHEA:50594"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>20-hydroxy-leukotriene B4 + NADP(+) = 12-oxo-20-hydroxy-leukotriene B4 + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:51208"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57460"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:133346"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="29">
            <dbReference type="Rhea" id="RHEA:51209"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>4-hydroxynonanal + NADP(+) = (E)-4-hydroxynon-2-enal + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:64736"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58968"/>
            <dbReference type="ChEBI" id="CHEBI:156112"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="28">
            <dbReference type="Rhea" id="RHEA:64738"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="30">
            <text>6-trans-leukotriene B4 + NADP(+) = 12-oxo-(5S)-hydroxy-(6E,8E,10E,14Z)-eicosatetraenoate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:51204"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:90723"/>
            <dbReference type="ChEBI" id="CHEBI:133974"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="30">
            <dbReference type="Rhea" id="RHEA:51205"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="33">
            <text>an n-alkanal + NADP(+) = an alk-2-enal + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13737"/>
            <dbReference type="ChEBI" id="CHEBI:12834"/>
            <dbReference type="ChEBI" id="CHEBI:13757"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.3.1.74"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="33">
            <dbReference type="Rhea" id="RHEA:13739"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>decanal + NADP(+) = (2E)-decenal + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50612"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:31457"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:133455"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="21">
            <dbReference type="Rhea" id="RHEA:50614"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>dodecanal + NADP(+) = (2E)-dodecenal + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50784"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:27836"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:133741"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="25">
            <dbReference type="Rhea" id="RHEA:50786"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="34">
            <text>hexanal + NADP(+) = (E)-hex-2-enal + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50776"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28913"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:88528"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="34">
            <dbReference type="Rhea" id="RHEA:50778"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>leukotriene B4 + NADP(+) = 12-oxo-leukotriene B4 + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50608"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57461"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:133309"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:50609"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>nonan-2-one + NADP(+) = (3E)-nonen-2-one + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50616"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:77927"/>
            <dbReference type="ChEBI" id="CHEBI:133457"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="26">
            <dbReference type="Rhea" id="RHEA:50618"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>octanal + NADP(+) = (2E)-octenal + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50780"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17935"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:61748"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="20">
            <dbReference type="Rhea" id="RHEA:50782"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>pentan-2-one + NADP(+) = (E)-pent-3-en-2-one + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50788"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16472"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:145276"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="22">
            <dbReference type="Rhea" id="RHEA:50790"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer or homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NADP-dependent oxidoreductase L4BD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="36">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.48" evidence="4"/>
    <dbReference type="EC" id="1.3.1.74" evidence="5"/>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YJ34"/>
    <dbReference type="OrthoDB" id="809632at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047522">
        <property type="term" value="F:15-oxoprostaglandin 13-reductase [NAD(P)+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032440">
        <property type="term" value="F:2-alkenal reductase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006693">
        <property type="term" value="P:prostaglandin metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08294">
        <property type="entry name" value="leukotriene_B4_DH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000121">
        <property type="entry name" value="Prostaglandin reductase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041694">
        <property type="entry name" value="ADH_N_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045010">
        <property type="entry name" value="MDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014190">
        <property type="entry name" value="PTGR1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43205">
        <property type="entry name" value="PROSTAGLANDIN REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43205:SF7">
        <property type="entry name" value="PROSTAGLANDIN REDUCTASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16884">
        <property type="entry name" value="ADH_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0007">Acetylation</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="15" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="14" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-0521">NADP</keyword>
    <keyword evidence="13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-0644">Prostaglandin metabolism</keyword>
    <keyword evidence="37" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="35">
        <location>
            <begin position="15"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00020651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00031851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00032255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00032297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00033119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00047617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00047871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00047878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00047903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00048066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00048290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00048387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00048591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00048953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00049068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00049070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="ARBA" id="ARBA00049179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="ARBA" id="ARBA00049368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="35">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="36">
        <source>
            <dbReference type="EMBL" id="KAJ8023473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="37">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="345" mass="37893" checksum="629553E1B2ECF2AF" modified="2023-09-13" version="1">MVKAKQWILKKQFSGTPKRTDVEIEEIMLPQLKDGEVLTECLVISVDPYQRLFVTHSKVSVGDVMYAEQVAKVIDSKHPDFPIGTHVTTFAGWTTHSIQTADKLTKIPDYPRDIPVSLALGILGMPGLTAYYGLLEVGKIKEGETVYVNAAAGAVGTIVGQIAKLKGCKVIGSAGSDKKVKYLKDELGFDEAFNYKTISDLPNLDKTLKRLIPQGIDVFFDNVGGGAFAVVMDNMNKKGRVALCGAISTYNDEDRYRLAIPNFTTSCGFQNVTIEGFTGWGFMELDMDNVALKEMISWVLGGKLKYPGHNFKGFENTFTAFLSLFSGDNVGKVLVDVKKCHLSQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YME6</accession>
    <name>A0A9Q0YME6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">dolichyl-P-Man:Man5GlcNAc2-PP-dolichol alpha-1,3-mannosyltransferase</fullName>
            <ecNumber evidence="3">2.4.1.258</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_38782</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>an alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate = an alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:29527"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="Rhea" id="RHEA-COMP:19516"/>
            <dbReference type="Rhea" id="RHEA-COMP:19517"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:132515"/>
            <dbReference type="ChEBI" id="CHEBI:132516"/>
            <dbReference type="EC" id="2.4.1.258"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:29528"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.258" evidence="3"/>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YME6"/>
    <dbReference type="OrthoDB" id="20028at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052925">
        <property type="term" value="F:dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007873">
        <property type="entry name" value="Glycosyltransferase_ALG3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12646:SF0">
        <property type="entry name" value="DOL-P-MAN:MAN(5)GLCNAC(2)-PP-DOL ALPHA-1,3-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12646">
        <property type="entry name" value="NOT56 - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05208">
        <property type="entry name" value="ALG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="88"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="111"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="183"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="225"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="278"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="358"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="389"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8021541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="424" mass="49713" checksum="2B8046412ABCD7C2" modified="2023-09-13" version="1">MPKKTRKSYAGEVFNKLCKMRVKDVIFDPDYLWITACVLFLAEIVVNIIIIKKVKYTEIDWEAYMSEVEGVVNGTYDYMQLKGGTGPLVYPAGFVYIFMALYYITDKGTDIRLAQYLFAVLYLATLLLVFRIFQKTKKVPPYVMFFVCCASYRIHSIYVLRLFNDPVAMFFFYLATNYFINRSWNWGCFWFSIAVSVKMNILLFAPALLLLLLTEFGFWRTIPRLAICGGVQVALAVPFLLENPAGYLLRAFELSRQFFYKWTVNWRFLSEEMFLNRYFQFSLLALHIILIILFTVHRWNRRSGGLTALLPKPTLPQREILDENYIVKTLYTSNFIGMAFSRTLHYQFYVWYFHTLPYLLWCTDLISPVRLLILGLIELCWNTYPSTSASSATLHVCHLILLVGLWLKKDVRQPEKLEQKGKQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YNH3</accession>
    <name>A0A9Q0YNH3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">SEC23-interacting protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_33332</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PA-PLA1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YNH3"/>
    <dbReference type="OrthoDB" id="69269at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004620">
        <property type="term" value="F:phospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004177">
        <property type="entry name" value="DDHD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058055">
        <property type="entry name" value="PA-PLA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004170">
        <property type="entry name" value="WWE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037197">
        <property type="entry name" value="WWE_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057825">
        <property type="entry name" value="WWE_SEC23-DDH2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23509:SF10">
        <property type="entry name" value="LD21067P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23509">
        <property type="entry name" value="PA-PL1 PHOSPHOLIPASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02862">
        <property type="entry name" value="DDHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02825">
        <property type="entry name" value="WWE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23464">
        <property type="entry name" value="WWE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01127">
        <property type="entry name" value="DDHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117839">
        <property type="entry name" value="WWE domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51043">
        <property type="entry name" value="DDHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50918">
        <property type="entry name" value="WWE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WWE" evidence="4">
        <location>
            <begin position="258"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="DDHD" evidence="5">
        <location>
            <begin position="778"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="28"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="706"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="916"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="53"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="64"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="119"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="156"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8025705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1018" mass="112940" checksum="7CA01C38E3D6BB03" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YNQ9</accession>
    <name>A0A9Q0YNQ9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein mL43</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_33465</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mL43 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YNQ9"/>
    <dbReference type="OrthoDB" id="88at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039927">
        <property type="entry name" value="Ribosomal_mL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007741">
        <property type="entry name" value="Ribosomal_mL43/mS25/NADH_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21396">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21396:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05047">
        <property type="entry name" value="L51_S25_CI-B8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00916">
        <property type="entry name" value="L51_S25_CI-B8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 8" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal protein/NADH dehydrogenase" evidence="7">
        <location>
            <begin position="36"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8025807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="195" mass="22527" checksum="82F1BC7A36C4F066" modified="2023-09-13" version="1">MGSTKKLSMSFLNSVLHNGVGRYVCQLQRLTLQFCKKHQFSRGMRDFIEENVVDFAHRNPQIVLYVTPHTKYQSPKIIAEYLNGKVNSVDVAKKEREEIAEIVDLLRTQSGQEILKTVKPWRSQSPSTQGIWHPFMHKPQELNLETFPSDKATFRGPYRPYEVLKNLSPELQEELAQLKARSLEAAQKERLEADS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YPN7</accession>
    <name>A0A9Q0YPN7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Pancreatic lipase-related protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_34041</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8026248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YPN7"/>
    <dbReference type="OrthoDB" id="6019621at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016298">
        <property type="term" value="F:lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00707">
        <property type="entry name" value="Pancreat_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002334">
        <property type="entry name" value="Allerg_PlipaseA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013818">
        <property type="entry name" value="Lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033906">
        <property type="entry name" value="Lipase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000734">
        <property type="entry name" value="TAG_lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610">
        <property type="entry name" value="LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610:SF178">
        <property type="entry name" value="LIPASE MEMBER H-A-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00151">
        <property type="entry name" value="Lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00825">
        <property type="entry name" value="DOLALLERGEN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00821">
        <property type="entry name" value="TAGLIPASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040251690" evidence="5">
        <location>
            <begin position="28"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="Lipase" evidence="6">
        <location>
            <begin position="29"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8026248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="350" mass="39114" checksum="116BFCED9E8390B0" modified="2023-09-13" version="1">MMVLCFDMCLKMLPLATVLLWTTNVGAKSVCYSGLGCFDDNLRCNSRRLPQSPSDINTKFYLYTRENRQTYQVIDWTNDASIVNSKFRGSRETKFIIHGFRDRIMTPDSDFLDMKDSFLSIGNFNVILVSWKDGADLFDLNGLLGHLLNLPIGYLQARQNARLVGRQVGELSRKLQQLTGASLSSMHLIGHSLGAHVAGFAGEHVSGKYGRITGLDPAGPGYQPYTSTAGCLLEKSDAIFVDIIHTDGILGNENAVGHQDFYPSGGKNQISCSFLDFICDHMRAVDFFTESLDPNCKWNAYPCSSWTFFKLGFCKRCGKKGCSRMGYYADRSKSTGKFMLETNKDKPYCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YQQ2</accession>
    <name>A0A9Q0YQQ2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peptidyl-prolyl cis-trans isomerase</fullName>
            <shortName evidence="1">PPIase</shortName>
            <ecNumber evidence="1">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_31663</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclophilin-type PPIase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="1"/>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YQQ2"/>
    <dbReference type="OrthoDB" id="408413at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000048">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071:SF561">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="2">
        <location>
            <begin position="124"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8026743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="292" mass="33063" checksum="B1FACECF912E10DC" modified="2023-09-13" version="1">MASRTQILVVGLLTDPTFHMAKCCAQGLAMTDEKKFCEPQIQGLLEFDWDMFCEQQKKALRGETWAFDDKAIVFVNGELLGGPKEFSKWASDTYVEYKPEALYLAMAKEAYQEHLESTGNTFVFFDISVGSENIGRLLLQLYTDKCPKTCENFRSLCTGERGIKKDEHLMTYHYKDTLLHRMVPNGWIQGGDILYGKGDGGESVYGAVFEDENFCVPHDQRGILGMANKGRHTNGSQFYITFQPAPWMDTKYVAFGRVVEGTNVLKILESQETYNERPKADCKIIDCGVFVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YR12</accession>
    <name>A0A9Q0YR12_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Protein ALP1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_35123</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HARBI1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8025034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YR12"/>
    <dbReference type="OrthoDB" id="10051515at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045249">
        <property type="entry name" value="HARBI1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027806">
        <property type="entry name" value="HARBI1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930:SF269">
        <property type="entry name" value="NUCLEASE HARBI1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13359">
        <property type="entry name" value="DDE_Tnp_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE Tnp4" evidence="8">
        <location>
            <begin position="4"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8025034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="229" mass="26067" checksum="1DEBAAC671F811D9" modified="2023-09-13" version="1">MLALYYYNYKGFHSTVLMAVADATYKFLHVDVGAEGGASDGGTWSNCSLHDVVEENRAGVPQPEPLPNDQPVPYHFVGDDAFALRTWMMKPFSHRSQVLRERIYSYRLSRARRVVENAFGILCNRFRCFLTTMHQHPGTINLITMCACVLHNLILIRYPHALSEVDGEDPDTNDLIPGGWRTDQQRQGMLPLTGHHTQKDAKDQRDYLSHYYMSPAGAVPWQERMVVAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YRX5</accession>
    <name>A0A9Q0YRX5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_31569</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YRX5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8026666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="63" mass="7392" checksum="9B01A03B2C5C2D0F" modified="2023-09-13" version="1">MLCMHRYRGRNLIFLLLFHGTALLPKLLFRIRFYCSGAQYLGIRPVRAHVHTAKCKLLSTVFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YS94</accession>
    <name>A0A9Q0YS94_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Collectin-12</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_34531</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YS94"/>
    <dbReference type="OrthoDB" id="8950604at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050111">
        <property type="entry name" value="C-type_lectin/snaclec_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22803">
        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0768">Sushi</keyword>
    <feature type="domain" description="Sushi" evidence="5">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="4">
        <location>
            <begin position="55"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8024586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="181" mass="21215" checksum="1A09010803E2A5BD" modified="2023-09-13" version="1">MSSLRIGDVCSMKCDKGFYRTLSNHTICERGSHDQEGHWSDGIFECHECIKWAEWNGSMYRFINTTQNLEESKMICKEQSYYDAHLVYIEDEDENDFITNMILQCGSSWSAWIGLSDEDEEDKWKWGGSNAEYLNWGIYDGRNEPNGGENENYVFINYYGMGKWYDVGPNRNIHAVCEREM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YSA3</accession>
    <name>A0A9Q0YSA3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Deleted in malignant brain tumors 1 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_31533</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YSA3"/>
    <dbReference type="OrthoDB" id="536948at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000001">
        <property type="entry name" value="Lysyl oxidase 4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000005">
        <property type="entry name" value="Neurotrypsin isoform A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48071:SF18">
        <property type="entry name" value="DELETED IN MALIGNANT BRAIN TUMORS 1 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48071">
        <property type="entry name" value="SRCR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00530">
        <property type="entry name" value="SRCR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00258">
        <property type="entry name" value="SPERACTRCPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00420">
        <property type="entry name" value="SRCR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040474644" evidence="7">
        <location>
            <begin position="22"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="471"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="8">
        <location>
            <begin position="29"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="8">
        <location>
            <begin position="144"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="8">
        <location>
            <begin position="259"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="8">
        <location>
            <begin position="366"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="535"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="559"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="53"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="66"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="97"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="169"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="182"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="213"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="284"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="328"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="391"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="435"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8026640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="580" mass="64433" checksum="309B222ED36A131C" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YT13</accession>
    <name>A0A9Q0YT13_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RecQ-mediated genome instability protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_32330</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. Promotes TOP3A binding to double Holliday junctions (DHJ) and hence stimulates TOP3A-mediated dissolution. Required for BLM phosphorylation during mitosis. Within the BLM complex, required for BLM and TOP3A stability.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RMI1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YT13"/>
    <dbReference type="OrthoDB" id="341511at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016604">
        <property type="term" value="C:nuclear body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031422">
        <property type="term" value="C:RecQ family helicase-topoisomerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000712">
        <property type="term" value="P:resolution of meiotic recombination intermediates"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.770:FF:000002">
        <property type="entry name" value="recQ-mediated genome instability protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.510">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.1020">
        <property type="entry name" value="RecQ-mediated genome instability protein 1, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.770">
        <property type="entry name" value="RecQ-mediated genome instability protein Rmi1, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032199">
        <property type="entry name" value="RMI1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049363">
        <property type="entry name" value="RMI1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042470">
        <property type="entry name" value="RMI1_N_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044881">
        <property type="entry name" value="RMI1_N_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013894">
        <property type="entry name" value="RMI1_OB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14790:SF15">
        <property type="entry name" value="RECQ-MEDIATED GENOME INSTABILITY PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14790">
        <property type="entry name" value="RECQ-MEDIATED GENOME INSTABILITY PROTEIN 1 RMI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16099">
        <property type="entry name" value="RMI1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08585">
        <property type="entry name" value="RMI1_N_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21000">
        <property type="entry name" value="RMI1_N_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01161">
        <property type="entry name" value="DUF1767"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RMI1 N-terminal" evidence="10">
        <location>
            <begin position="14"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="RecQ mediated genome instability protein 1 OB-fold" evidence="8">
        <location>
            <begin position="68"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="RecQ-mediated genome instability protein 1 C-terminal OB-fold" evidence="9">
        <location>
            <begin position="658"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="215"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
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    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="482"/>
            <end position="544"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="243"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="273"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="385"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="442"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="485"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="504"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="521"/>
            <end position="537"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF21000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8027240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="806" mass="89077" checksum="BC3536BF5AE3A35B" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YT71</accession>
    <name>A0A9Q0YT71_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_31455</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YT71"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="24"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8026579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="114" mass="12763" checksum="229EF86D4D5003AC" modified="2023-09-13" version="1">MPDIIPTCGKRIRHVENTVRDLPVALTVGLGAGVALCFVILLVTSVAIFARYTYHDDRKKMPPHDSNKEDNISDPSAPYLPNDDEPKHYSANTPFKPMPRIKPPPENNHSITYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YTV9</accession>
    <name>A0A9Q0YTV9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Protein jagunal-like 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_32166</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the jagunal family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YTV9"/>
    <dbReference type="OrthoDB" id="8914197at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007029">
        <property type="term" value="P:endoplasmic reticulum organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009787">
        <property type="entry name" value="Jagunal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20955">
        <property type="entry name" value="PROTEIN JAGUNAL HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20955:SF1">
        <property type="entry name" value="PROTEIN JAGUNAL HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07086">
        <property type="entry name" value="Jagunal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="72"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="104"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="140"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8027117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="178" mass="19835" checksum="C976B46B725764B2" modified="2023-09-13" version="1">MASRYGAREPGTDGSDFRYREQVASKHTTSLAVKGRLKRYFFLNIILSVVQLILLVVTALDLVEDATKLQPAPWQAMWMLSGIPSIIGLSALPKNRSGLLKIHNAGLIVFGVGGIVWGVVSNYENMVNLWEGNPTEEIFGLPKVLIVYNSAVVAFALYMSTIFNTRTLLSVWDYRKTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1B957</accession>
    <name>A0A9Q1B957_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_43058</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000197">
        <property type="protein sequence ID" value="KAJ8018771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1B957"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8018771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="94" mass="10580" checksum="6B9D7F8B6541A66A" modified="2023-09-13" version="1">MNATSSRSHAISRTVKKRRQVRKGTLLPYSKSSAGRTTGATAMNSTSSRSHPIFTIQDERKEDRLVNFISTDSEEVELGATFLLYSFFPKVCYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1B9N9</accession>
    <name>A0A9Q1B9N9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_43549</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000355">
        <property type="protein sequence ID" value="KAJ8018455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1B9N9"/>
    <dbReference type="OrthoDB" id="5950997at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319:SF35">
        <property type="entry name" value="OUTER MEMBRANE PROTEIN PMPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01411">
        <property type="entry name" value="Ephrin_rec_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="196"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="306"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="351"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="411"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="541"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="572"/>
            <end position="595"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8018455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="602" mass="69309" checksum="A258A642FCA5F63C" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BBM2</accession>
    <name>A0A9Q1BBM2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_42421</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000071">
        <property type="protein sequence ID" value="KAJ8019174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BBM2"/>
    <dbReference type="OrthoDB" id="10598211at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8019174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="144" mass="16954" checksum="EF626EE0CEC93DE8" modified="2023-09-13" version="1">MFRVWGPVDVERYLGCVPPRSFSVGPTLSTLKSRNVEVLWRWTGDTPRCTLNLQSGRWENEDDGSEPTEEVFQRAFSRLAELRTGWTNKTYRETLYCLKLRYRSVNNYTGANEVKIGLGQFEKTLRFKQRQERERVKGDREGLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BBS4</accession>
    <name>A0A9Q1BBS4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Latrophilin Cirl</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_39379</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8022011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BBS4"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.220.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057244">
        <property type="entry name" value="GAIN_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046338">
        <property type="entry name" value="GAIN_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000203">
        <property type="entry name" value="GPS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12011">
        <property type="entry name" value="ADHESION G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12011:SF347">
        <property type="entry name" value="FI21270P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01825">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00303">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50221">
        <property type="entry name" value="GAIN_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="150"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="GAIN-B" evidence="7">
        <location>
            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8022011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="214" mass="24189" checksum="A50C40AABD8B1E87" modified="2023-09-13" version="1">MSFPSVSLNTETVSNEHCVVPNYRKRSLTISDVPYISGDLVDITKAKTIGNADITHTSTVLNMISSVYSTSQLVECFTFVCANKFNSKFTCVFWDHILHEESAGWSSEGCHNEEANDSYVYICKCDHFGNMAVLVFLTNDIEVRSSNNTVLFVCIIVVIIIVATICTYLAIEFPFYSLLSNGADIFVQHARRRVFRYQENAINHDMFEPLELHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BC45</accession>
    <name>A0A9Q1BC45_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_41475</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8019759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BC45"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8019759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="68" mass="7551" checksum="5CC42FBBFF824515" modified="2023-09-13" version="1">MGSRRELTSRTSFLAHARLETGVAAREFRHWNEKSRAAARACLASSPPCRHVRLPGELEVSQAHGEIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BCH7</accession>
    <name>A0A9Q1BCH7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_40061</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BCH7"/>
    <dbReference type="OrthoDB" id="10059338at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33480">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33480:SF1">
        <property type="entry name" value="TYR RECOMBINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8020463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="385" mass="43911" checksum="775ED5B87859EF74" modified="2023-09-13" version="1">MDPQKRRHHVAQKMRESARLLVNFRSLAENDASMDDMLSPQNYNNVIKAVEMTGGVTTVDSVKHPSTILKLGNDLQKLAKVKRALSIIAKDNLRKEEANDFIQLVSTDFTDKMTAPALSTLRQRKFEKANDMPESEDVHKFSKFLSTEVKKSIKILNDAKDHGLEKRSAAYRRAQHLALAKLTFFNKRRTGETEQLSLKAYNNTGECDIKEIMDTLSPVERELCRTLKVVFIRGKRGRKVPLIIPQDCAELMDLLRDLRQSAGISSKNKYFFGRFGARTPIRATDSLRELTLEAQLNSPHLVRSTKMRKYTATLSQVFSLEQQHTEWLTEHLGHSVKVHREHYRLPSSTIEKAKIAKLLLAIDSGLTRKFYGKSLDEITFDGISL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BDG6</accession>
    <name>A0A9Q1BDG6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Sushi domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_40229</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BDG6"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sushi" evidence="2">
        <location>
            <begin position="37"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8020597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="95" mass="10629" checksum="5953E7655DB14D9E" modified="2023-09-13" version="1">MNVSLFTHRKNPRLWFLQFCHLTVSVPLCSYADLPVIASGHFYVESCSGKEVGDVCLMQCEDNYYPATDIETRCQISGSAKAAWSFPEDGFHCTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BED9</accession>
    <name>A0A9Q1BED9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Zinc finger CCHC domain-containing protein 7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">TRAMP-like complex RNA-binding factor ZCCHC7</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_37263</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BED9"/>
    <dbReference type="OrthoDB" id="7608935at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031499">
        <property type="term" value="C:TRAMP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071031">
        <property type="term" value="P:nuclear mRNA surveillance of mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071039">
        <property type="term" value="P:nuclear polyadenylation-dependent CUT catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071035">
        <property type="term" value="P:nuclear polyadenylation-dependent rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071036">
        <property type="term" value="P:nuclear polyadenylation-dependent snoRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071037">
        <property type="term" value="P:nuclear polyadenylation-dependent snRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051644">
        <property type="entry name" value="TRAMP_AT-DNA-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025836">
        <property type="entry name" value="Zn_knuckle_CX2CX4HX4C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46543">
        <property type="entry name" value="ZINC FINGER CCHC DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46543:SF1">
        <property type="entry name" value="ZINC FINGER CCHC DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14392">
        <property type="entry name" value="zf-CCHC_4"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="11">
        <location>
            <begin position="271"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="11">
        <location>
            <begin position="312"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="11">
        <location>
            <begin position="380"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="168"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="518"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="172"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="182"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="541"/>
            <end position="554"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00041190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00043023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8022383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="619" mass="71502" checksum="9CA87E054AE5ACEF" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BF71</accession>
    <name>A0A9Q1BF71_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_35595</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BF71"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="118"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8023238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="146" mass="17329" checksum="02C110B131BC69E9" modified="2023-09-13" version="1">MEKLTQDQLTRKSLFELWKSETTSEEKRSQSILKSKEDWLRNLPSQEENNTTAPDYPSQHDRTYPRPRVPTDGQYHHPRRSFRKAHYVHRNGHFDTHSQPSPTPRFYQTSITATFTPHHRAHRAQHRNTYQSPTALPGKTTREKQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BFD6</accession>
    <name>A0A9Q1BFD6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Calcineurin-like phosphoesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_33366</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BFD6"/>
    <dbReference type="OrthoDB" id="550558at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00838">
        <property type="entry name" value="MPP_superfamily"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052963">
        <property type="entry name" value="Pantetheine_PDE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36492">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36492:SF2">
        <property type="entry name" value="[ACYL-CARRIER-PROTEIN] PHOSPHODIESTERASE PPTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="1">
        <location>
            <begin position="3"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8025731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="313" mass="36774" checksum="58726A0523B75969" modified="2023-09-13" version="1">MARIFALSDLHVDNLDNMYFVERLSFEKYKDDVLIVAGDVTDNVTLLKSTFLILKKKFKEVVFVPGNHDIWIRERDSWETSDGKFHRLLRLCKALDVKTDPLKVRLRDNTDVWIVPLFSWYSTPEEDAKDSLYIQDPSITSPKDDWSKQMWMDFHHCVWPDDMDETPAKYFSDLNTERCKWNYDAPVISLSHMLPRRELILADADDHRELIKERQRRGLPPVIPNRQGATEHFNFTRFAGSQTIEKQIRQIGSVMHVYGHQHRNRDRTIEGVRYVSHCLGSKHERAKGLIAGMYKWNGPKQIYPKDAINSIEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BHV4</accession>
    <name>A0A9Q1BHV4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_31597</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BHV4"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8026688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="77" mass="8968" checksum="C482F7AAB586ABF7" modified="2023-09-13" version="1">MRAKVASVLSRQFRDMAVCRLNRSSPTFQFPMHTKREKHCVTVEVKQQNPEFAEEKRGCKGLLYGQHRTIASDYLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BI22</accession>
    <name>A0A9Q1BI22_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Coiled-coil domain-containing protein 172</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_33437</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CCDC172 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BI22"/>
    <dbReference type="OrthoDB" id="10055570at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029618">
        <property type="entry name" value="CCDC172"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22419">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22419:SF2">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="367"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="11"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="114"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="383"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8025787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="393" mass="45176" checksum="592331F0A042D068" modified="2023-09-13" version="1">MIISFTVQGKIEETQNKLKETDVELVTLKGQFAIKLQQLSEEELDLKWIKNKKKIMEEQAKELKEKVEKQSLLLKEKTKEADDEREAFMGRVSSFICNFGLCGNGKRIRDAEANQKLEELRKSEMDLLTELKELRRKQEEVDDLQLEKTTLMEEIKTYKDRVAELEQKIDSEKTKTKGISDEKQCLAKRPETGQEFQRLQQELDTCRRDSLEGLCQALQPEVDRLQRQAWQRQIQIPHRPTVSSSSSIPVTLRGRLPPLSVHQGTRALSSSKRHFTTARSLMSEMQPSGQWKSKQVGGTSWETSKMIIPPEPPLLEDSPPKADGSKTIENETVVKLSDSNMASKEEQQPVFTIEEVLISDDEDLFEDDILESPGKPSDSSRRFPQKAKRVRFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BI52</accession>
    <name>A0A9Q1BI52_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Adhesion G-protein coupled receptor D1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_34163</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8026344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BI52"/>
    <dbReference type="OrthoDB" id="1100386at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.220.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046338">
        <property type="entry name" value="GAIN_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017983">
        <property type="entry name" value="GPCR_2_secretin-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000203">
        <property type="entry name" value="GPS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12011">
        <property type="entry name" value="ADHESION G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12011:SF471">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 2 PROFILE 2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01825">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00249">
        <property type="entry name" value="GPCRSECRETIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00650">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="554"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="592"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="622"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="660"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="709"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="756"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="785"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="7">
        <location>
            <begin position="556"/>
            <end position="806"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8026344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="869" mass="98408" checksum="2CC54C2CD416DE8B" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BJ72</accession>
    <name>A0A9Q1BJ72_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Death domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_32694</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BJ72"/>
    <dbReference type="OrthoDB" id="6118651at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.220.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000488">
        <property type="entry name" value="Death_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28336">
        <property type="entry name" value="BA1-643"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28336:SF4">
        <property type="entry name" value="DEATH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00531">
        <property type="entry name" value="Death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50017">
        <property type="entry name" value="DEATH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Death" evidence="2">
        <location>
            <begin position="1048"/>
            <end position="1126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="190"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="254"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="489"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="723"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="976"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="274"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="286"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="313"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="338"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="355"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="375"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="404"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="489"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="508"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="522"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="723"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="733"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="976"/>
            <end position="995"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8027529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1136" mass="127073" checksum="CE845E0B75EB4726" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BJL9</accession>
    <name>A0A9Q1BJL9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_29920</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8027848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BJL9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8027848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="52" mass="5777" checksum="67C62F2AD508A2D6" modified="2023-09-13" version="1">MPHIERKNPIVFGGGQRSSGVTGGQTLKTLLTRYLKKGSSYSSQTWYIDAPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BKS0</accession>
    <name>A0A9Q1BKS0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Helix-turn-helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_30738</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BKS0"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058912">
        <property type="entry name" value="HTH_animal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21301">
        <property type="entry name" value="REVERSE TRANSCRIPTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21301:SF10">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26215">
        <property type="entry name" value="HTH_animal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="55"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="193"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="Helix-turn-helix" evidence="3">
        <location>
            <begin position="85"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF26215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8028488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="214" mass="24445" checksum="B8C1648B59A55792" modified="2023-09-13" version="1">MRCGQPPSCSPPSPNQPVYTSPFDNSVPQEENQPPVLVRNVQYYEPDPPGLSRTITFSLVLSSIALIFVVPMFCAIPALIFANLYESFQRHHIKSSLIYSQAIRLKRICSDPVDFEHRIHDLSMQLIDRGYPLSLIKEQIAKARLATRESLLRYNRKRESDNRIPFVALSKRYGVKQRQSYANTALNLTKTSYLTIVVFCVIGMVFRVAVGYFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BLE2</accession>
    <name>A0A9Q1BLE2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_28086</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8028848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BLE2"/>
    <dbReference type="OrthoDB" id="6426891at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8028848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="142" mass="16018" checksum="B662F5537D9B6C4E" modified="2023-09-13" version="1">MSFTLPDFQGPTGAHRTPRQARRFPEQQGATSTGPRREPTVTVASRSERNFPRAQDRLTHVQLLFTLKPSPQLGPPGSRWSICYCHQDLHPRRLHAGSRPDRFKAHTGVLPTRREHARRKANSSPDGPARPPRSAPFIFRAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BLL7</accession>
    <name>A0A9Q1BLL7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Lengsin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Glutamate-ammonia ligase domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_28180</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">May act as a component of the cytoskeleton or as a chaperone for the reorganization of intermediate filament proteins during terminal differentiation in the lens. Does not seem to have enzymatic activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Dodecamer. Interacts with BFSP2 and VIM.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the glutamine synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8028922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BLL7"/>
    <dbReference type="OrthoDB" id="77835at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004356">
        <property type="term" value="F:glutamine synthetase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006542">
        <property type="term" value="P:glutamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.590.10:FF:000009">
        <property type="entry name" value="Lengsin, lens protein with glutamine synthetase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.70:FF:000007">
        <property type="entry name" value="LOW QUALITY PROTEIN: lengsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.70">
        <property type="entry name" value="Glutamine synthetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.590.10">
        <property type="entry name" value="Glutamine synthetase/guanido kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008147">
        <property type="entry name" value="Gln_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036651">
        <property type="entry name" value="Gln_synt_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008146">
        <property type="entry name" value="Gln_synth_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43407">
        <property type="entry name" value="GLUTAMINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43407:SF1">
        <property type="entry name" value="LENGSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00120">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01230">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54368">
        <property type="entry name" value="Glutamine synthetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51986">
        <property type="entry name" value="GS_BETA_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51987">
        <property type="entry name" value="GS_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GS beta-grasp" evidence="8">
        <location>
            <begin position="21"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="GS catalytic" evidence="9">
        <location>
            <begin position="122"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00037583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00039404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00042675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8028922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="460" mass="51421" checksum="C906FA28329031C1" modified="2023-09-13" version="1">MTMTENNRDLLENCLRKITEAEIKHIRFEVSDLHGIARSKTIPARHFQSLAKSGILFPACIYGMDSVDEVIGDENIQKNNWSDMMCYPDLTTFTVLPWVKNTARVLISPSTACNGVEIAVDPRNLALKQLSALKQKNLSILYSFEYEFWVLHAATRKPITDHHNSLATLRLAKHQEFFDRITENLYGAGIDVECVHSEHAFGHVEMPISPAFGIKSADNAATFRTGVKEMAIQDNYVASFMTKPDEGQDGAGAHLNHSLWSVDGKTPKFSDPSRPYGLSDIGEHWIAGLLAHAPALSLLQAPTLNCRHRFEPGVFAPINATWGVDNRSCAIRWKERGHHGSHVEDRIGSSAGNPYISLAAMIAAGIDGIERQLPLPKYVPFDKSAQDEKNIPPGTELLPTKTEDALNALLSDQVMVTALGQEFVAFFEGLKRHEMKLFQEATQWGGSKINWEKRYYFEYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BM75</accession>
    <name>A0A9Q1BM75_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C2HC/C3H-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_28539</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BM75"/>
    <dbReference type="OrthoDB" id="265955at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="7"/>
    </dbReference>
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        <property type="entry name" value="ZC2HC1A/B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049899">
        <property type="entry name" value="Znf_C2HC_C3H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13555">
        <property type="entry name" value="C2H2 ZINC FINGER CGI-62-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13555:SF68">
        <property type="entry name" value="ZINC FINGER PROTEIN 474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13913">
        <property type="entry name" value="zf-C2HC_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52027">
        <property type="entry name" value="ZF_C2HC_C3H"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2HC/C3H-type" evidence="7">
        <location>
            <begin position="5"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="C2HC/C3H-type" evidence="7">
        <location>
            <begin position="145"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="C2HC/C3H-type" evidence="7">
        <location>
            <begin position="217"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="C2HC/C3H-type" evidence="7">
        <location>
            <begin position="289"/>
            <end position="318"/>
        </location>
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    <feature type="domain" description="C2HC/C3H-type" evidence="7">
        <location>
            <begin position="362"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="domain" description="C2HC/C3H-type" evidence="7">
        <location>
            <begin position="415"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="domain" description="C2HC/C3H-type" evidence="7">
        <location>
            <begin position="490"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="38"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="235"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="323"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="384"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="38"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="74"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="262"/>
            <end position="272"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="389"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS52027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8029201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="517" mass="57052" checksum="316056098009643B" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BNG9</accession>
    <name>A0A9Q1BNG9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Receptor-type tyrosine-protein phosphatase F</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_29165</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BNG9"/>
    <dbReference type="OrthoDB" id="5831260at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26391">
        <property type="entry name" value="INACTIVE TYROSINE-PROTEIN KINASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26391:SF18">
        <property type="entry name" value="PROTEIN KINASE RECEPTOR TIE-1, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040366754" evidence="2">
        <location>
            <begin position="18"/>
            <end position="1067"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="3">
        <location>
            <begin position="123"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="3">
        <location>
            <begin position="227"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="3">
        <location>
            <begin position="333"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="3">
        <location>
            <begin position="440"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="3">
        <location>
            <begin position="546"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="3">
        <location>
            <begin position="652"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="3">
        <location>
            <begin position="759"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="3">
        <location>
            <begin position="971"/>
            <end position="1067"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="205"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="305"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="413"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="520"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="626"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="732"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="910"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="422"/>
            <end position="441"/>
        </location>
    </feature>
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        <location>
            <begin position="743"/>
            <end position="758"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8029705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1067" mass="120898" checksum="8A408D43A9435642" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BPK9</accession>
    <name>A0A9Q1BPK9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_26622</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BPK9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF13">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="78"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8030260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="230" mass="27223" checksum="D55D2221899CC7D4" modified="2023-09-13" version="1">MPFNLDKFVASPSVEELDSLKKSEIVKVAKHYGIEFQPLMRKDEIKRYVLEYLVDESILPSTVLETAITVPTDNTFELKRLEIEMNKEIRLKEMEREREREERAMQKEKEEREHEFRLKQLELGVIKGSDPKIGLDTGGFDVSKHVKFVPKFQEDNVEKFFNHFEKLGEQLKWPRDKWSILIQSNFTGKAQEVYSALSIEDSMDYDKVKKAILQAYELVPEAYRQKFRKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BQ55</accession>
    <name>A0A9Q1BQ55_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ependymin-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_27107</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BQ55"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001299">
        <property type="entry name" value="Ependymin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10697">
        <property type="entry name" value="MAMMALIAN EPENDYMIN-RELATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10697:SF1">
        <property type="entry name" value="MAMMALIAN EPENDYMIN-RELATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00811">
        <property type="entry name" value="Ependymin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040411743" description="Ependymin-related protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8030639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="245" mass="27878" checksum="41CE0C6B360ADB9D" modified="2023-09-13" version="1">MASMLVLSLFALVLGVDFVASNDCCVADQFNVGFAGTKTKIIDSRKISFEPISDPSHNRRVRFQGEAAIDHIGHRYAANVSWYDYRQKTWGSIRFIKLINESTSYVIKDSKCFKKPACQREPPSCVPDDAYLDETMFIGENSLQLNTWRMRIGKQRPVHGDLRFATTQENCIPVFSSLIGRRKMNTTGEYARFEVDGVFFNYTEALSPSSFVIPDICHNATLTIKDKAFGLLEEWHTFQEGMVFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BQU2</accession>
    <name>A0A9Q1BQU2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Ileal sodium/bile acid cotransporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_27719</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bile acid:sodium symporter (BASS) (TC 2.A.28) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8031106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BQU2"/>
    <dbReference type="OrthoDB" id="203097at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002657">
        <property type="entry name" value="BilAc:Na_symport/Acr3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004710">
        <property type="entry name" value="Bilac:Na_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10361:SF65">
        <property type="entry name" value="ILEAL SODIUM_BILE ACID COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10361">
        <property type="entry name" value="SODIUM-BILE ACID COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01758">
        <property type="entry name" value="SBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0769">Symport</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="214"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="248"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="277"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="304"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="347"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="378"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="408"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="471"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8031106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="559" mass="61680" checksum="F2983AA16A54BFC8" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BS75</accession>
    <name>A0A9Q1BS75_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_25087</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BS75"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8031780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="65" mass="7168" checksum="39ED5E5FE6B2CEC3" modified="2023-09-13" version="1">MSTKLGGHYLFRQIGWAEGGGGGVKTVPPKSKLTLKSGIKFVSRYAFFRRPLCKCSFSISQMVIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BTG6</accession>
    <name>A0A9Q1BTG6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Histone H1.5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_26001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the histone H1/H5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BTG6"/>
    <dbReference type="OrthoDB" id="10069608at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00073">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005819">
        <property type="entry name" value="H1/H5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005818">
        <property type="entry name" value="Histone_H1/H5_H15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00538">
        <property type="entry name" value="Linker_histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00624">
        <property type="entry name" value="HISTONEH5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00526">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51504">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0158">Chromosome</keyword>
    <keyword evidence="1 2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="H15" evidence="4">
        <location>
            <begin position="16"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="101"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="101"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="127"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8032470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="197" mass="20890" checksum="1083FDC130D81D62" modified="2023-09-13" version="1">MATEVTEQKKKSSKPSGPKTIDLIKKAIVDLKEPKGSSVSAIKKQLTAMGVDVQKKNTIINKALKKGVTDGVLKQTKGTGASGTFKLDTAKALAAEKAKAKKAKAAERKRAAKEKAKVKKAEAKAKMAAKKAAAKKKSAAKKKKTPAKPKKAVKASKTVKKPAKKTAAKKKPVKKPAKKPAAKKPVKKVQKKKPAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BU88</accession>
    <name>A0A9Q1BU88_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Octopamine receptor beta-2R</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_22943</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8032872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BU88"/>
    <dbReference type="OrthoDB" id="5954629at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00637">
        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45698:SF1">
        <property type="entry name" value="TRACE AMINE-ASSOCIATED RECEPTOR 13C-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45698">
        <property type="entry name" value="TRACE AMINE-ASSOCIATED RECEPTOR 19N-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 8" id="KW-0675">Receptor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="146"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="7">
        <location>
            <begin position="38"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8032872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="181" mass="20952" checksum="D02D1B1061F21D80" modified="2023-09-13" version="1">METFDNVTAYNNATEPTLTAPVYLSGLILSIAILGIFDNGLVLIVIAKVPEFRRNMTNILVGHQSLIDFITAVLLLLTFQKFYKRNLDGRYHPVIENFFCKVWDTSFIYWSTYKVSTANLLCVTLERYFALVFPLQYRKHVTRRRIYLVCIFPWIIGPMSEAYVFFIFVVSPATGRETVLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BUJ2</accession>
    <name>A0A9Q1BUJ2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein quiver</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_22920</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8032854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BUJ2"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="185"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8032854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="204" mass="22642" checksum="89697CBFCCCF867B" modified="2023-09-13" version="1">MQRHEHSNIRLRLATIFIKFLNVVIEPRGNFQLKRNCSSIQCKEKMPSTGTFSLTICFAVLLTVRALQCYNKIEYDCSPSGPVCDAFPQTSMTVTECDSDVTMCGLINIGATAEDYKLHTSIASCLPQEDGVSLTSGCYGREDLEKIYPDKGKELNQLERVYRVTLDPVEICICDGNLCNGSIKSVSSISIIITSVVISFMLFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BUR4</accession>
    <name>A0A9Q1BUR4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_23274</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BUR4"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="61"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="147"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="169"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8033130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="188" mass="20929" checksum="802D5DEE67EF2FE5" modified="2023-09-13" version="1">MLEVLTWFGESIVLGDTVTCNSSIDTNQSSDGGFCNIAFNTNWTQSKCEADEQGSCVIPGWFFIVMAAFQGILLLILGGCLLVGWKYVTSQSKREHKPDFVPTRRQSNDYTPDDGTYLQPSFTNQGKEPPCIALSVSRTDQETYDRVPVRGGSANKRGKAKVNIEPEENVTHDSEEHYDFPSKIARHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BVB0</accession>
    <name>A0A9Q1BVB0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Retinol dehydrogenase 8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_23766</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BVB0"/>
    <dbReference type="OrthoDB" id="1274115at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43391">
        <property type="entry name" value="RETINOL DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43391:SF86">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE_REDUCTASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="77"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="108"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="8">
        <location>
            <begin position="88"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8033506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="403" mass="45131" checksum="F407F8EA28BB6902" modified="2023-09-13" version="1">MLHTKLEASLITLAYFLVITIEFNMSNKNHKVIVITGCSSGIGLETAVLLAKVKDYKWKELKHISEWNSPPLRPLPLPLPIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIVIATMRNLKERQVLEKAAKSNLNGPLVIKELDISKEDSIIKFVKGTIEKEGRIDVLINNAAVGQFGKFEDVSLEQMQNLFQTNFFGTVRITQEVIRKMKEQRSGKVIFVSSLAGVQPFMFCEFYVATKFALEGLVGCLAPLMRAFNIRRPMLFTRTPLLLVEVEGRMSITSLEPGPVKTEIGTNMIKNKQGSFSIEDETYDEETKSLLGNVFKNTEAEMQSEIQTGEEVAEVIKKCVQDDKPDIRVATSEFVKAKGEAILVDYSGNAIADTMEALLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BVD6</accession>
    <name>A0A9Q1BVD6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GIY-YIG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_24414</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8034008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BVD6"/>
    <dbReference type="OrthoDB" id="5975639at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="CDD" id="cd10442">
        <property type="entry name" value="GIY-YIG_PLEs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000305">
        <property type="entry name" value="GIY-YIG_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035901">
        <property type="entry name" value="GIY-YIG_endonuc_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058912">
        <property type="entry name" value="HTH_animal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21301">
        <property type="entry name" value="REVERSE TRANSCRIPTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21301:SF10">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26215">
        <property type="entry name" value="HTH_animal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82771">
        <property type="entry name" value="GIY-YIG endonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50164">
        <property type="entry name" value="GIY_YIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GIY-YIG" evidence="1">
        <location>
            <begin position="203"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8034008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="303" mass="34168" checksum="D750DA45F441FFB3" modified="2023-09-13" version="1">MGVEMSGDRITLKVLFDGPEVFSDSSNLHFKPTDSHNYLLYPSNHPKSCTESIPYSQLLRARRICSEDSDFSAASKSILSFFEKRQYPTKILTGALHRIQGIDRAEALSKKSNTSTNLRIPLVISHHPSVRPIIRAVYRNIETLRKDPSTRDLFPEPPITAFRVEKNISKHLVRASHPQQPTDNTPGPNNNRFNVHQHFTCTSANVVYVLVCKRCNILYVGETKRRLADRVTEHLRSIKQNLPGFPVATHFNPPSTCSIRDLMVSAAISCRGSDHDRLAAENRLIMKLGTLSPHGLNVRLELL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BWB6</accession>
    <name>A0A9Q1BWB6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Sepiapterin reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_21369</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BWB6"/>
    <dbReference type="OrthoDB" id="153074at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004757">
        <property type="term" value="F:sepiapterin reductase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006729">
        <property type="term" value="P:tetrahydrobiopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051721">
        <property type="entry name" value="Biopterin_syn/organic_redct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44085">
        <property type="entry name" value="SEPIAPTERIN REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44085:SF2">
        <property type="entry name" value="SEPIAPTERIN REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0521">NADP</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8034503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="192" mass="21599" checksum="2D628DB1EB01962D" modified="2023-09-13" version="1">MNRKIFETVGDVSRFSHAIIIHNAASLGIISKYAREMNDVSSIQQFFLLNMTAPIVMTSSFLKCTNGCRNSSLRKTVVQITSLAGSRPQKLMHLYCAAKSGRDMFFRSMAVEESKVRTLTYEPGAMDTMMFKDALKTNDTEFRANLDRISGEDFFLSPKESASVLVKLLEEDKYENADIVHCYDVLGLNVNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BX72</accession>
    <name>A0A9Q1BX72_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_21072</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BX72"/>
    <dbReference type="OrthoDB" id="6128564at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8034295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="121" mass="13839" checksum="6D79D41F83BFFB1D" modified="2023-09-13" version="1">MIPYSNERNPFIEKFTWKSNIIPGIFCRLDFFLISKHLKLNVRSDGFIPGFCSDHSFVVLELTAGTLKRGPGFWKMNNSLLNDDNYLNLINCALIRGLEIMQNAIKMGLFKVYGSYGNDPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BXF9</accession>
    <name>A0A9Q1BXF9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">SRCR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_21174</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BXF9"/>
    <dbReference type="OrthoDB" id="536948at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000007">
        <property type="entry name" value="Soluble scavenger receptor cysteine-rich domain-containing protein SSC5D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48071">
        <property type="entry name" value="SRCR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48071:SF28">
        <property type="entry name" value="SRCR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00530">
        <property type="entry name" value="SRCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00258">
        <property type="entry name" value="SPERACTRCPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00420">
        <property type="entry name" value="SRCR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="SRCR" evidence="7">
        <location>
            <begin position="13"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="38"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="51"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="82"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8034374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="133" mass="14378" checksum="80B8E264D73B4866" modified="2023-09-13" version="1">MLSRYTGDGIQGIRIADGLSNSSGRVEVFVNGAWGTVCDDSWDLPDAMVACKQLGYQAAIAVPRRAFFGNGTGPIWMDDVQCGGFEISLSKCSQPLLAKHNCHHSEDAGVVCGGKDTRVLFKETNRNYTYLGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BXR6</accession>
    <name>A0A9Q1BXR6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Serine/threonine-protein kinase/endoribonuclease IRE2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_21605</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BXR6"/>
    <dbReference type="OrthoDB" id="63989at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990604">
        <property type="term" value="C:IRE1-TRAF2-ASK1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070059">
        <property type="term" value="P:intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036498">
        <property type="term" value="P:IRE1-mediated unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045133">
        <property type="entry name" value="IRE1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13954">
        <property type="entry name" value="IRE1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13954:SF6">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="460"/>
            <end position="590"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8034672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="590" mass="67237" checksum="C8779421836ED211" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BYR0</accession>
    <name>A0A9Q1BYR0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_22635</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10055784at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01644">
        <property type="entry name" value="RT_pepA17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005312">
        <property type="entry name" value="DUF1759"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040676">
        <property type="entry name" value="DUF5641"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008042">
        <property type="entry name" value="Retrotrans_Pao"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331:SF1">
        <property type="entry name" value="GAG-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03564">
        <property type="entry name" value="DUF1759"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18701">
        <property type="entry name" value="DUF5641"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05380">
        <property type="entry name" value="Peptidase_A17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="2">
        <location>
            <begin position="1455"/>
            <end position="1641"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8035411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1758" mass="199830" checksum="102B3C698B6050D8" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BZE7</accession>
    <name>A0A9Q1BZE7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_19271</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8035557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BZE7"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="14"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8035557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="68" mass="7934" checksum="730831E93CD7581F" modified="2023-09-13" version="1">MLCILDTLWKPHECRQKKDDSQSKKYHATQVSQHRQLTIQDGQPSQDQKTAKTFHERSVPPEGENDVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C236</accession>
    <name>A0A9Q1C236_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NLR family CARD domain-containing protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_17749</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C236"/>
    <dbReference type="OrthoDB" id="427518at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312">
        <property type="entry name" value="NACHT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312:SF2">
        <property type="entry name" value="NUCLEOTIDE-BINDING OLIGOMERIZATION DOMAIN-CONTAINING PROTEIN 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NACHT" evidence="1">
        <location>
            <begin position="69"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8037030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="600" mass="69890" checksum="F4ABD1337FD76C85" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C4F6</accession>
    <name>A0A9Q1C4F6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">IgGFc-binding protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_18639</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C4F6"/>
    <dbReference type="OrthoDB" id="5945029at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035234">
        <property type="entry name" value="IgGFc-bd_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46534">
        <property type="entry name" value="IGGFC_BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46534:SF2">
        <property type="entry name" value="VWFD DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17517">
        <property type="entry name" value="IgGFc_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040176747" description="IgGFc-binding protein N-terminal domain-containing protein" evidence="1">
        <location>
            <begin position="33"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="IgGFc-binding protein N-terminal" evidence="2">
        <location>
            <begin position="142"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8037746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="216" mass="23619" checksum="DFCD54898AEBC8CA" modified="2023-09-13" version="1">MTFGGFNFCTMELLRKVPIIVAIFTFLNIAKGQPDPQCEGTNRGRSFVITFPGNYESDADSIELSVLVVALGDNTTSVTLSSMFDSFQSTLEIEPMQFSWVSIPSEFALSMKSEKSNKAIQVVADSEISVYGLNYQRTTTNAILGIPTDSLEDNYVQMGVVESIDSPSLFVVVGVKENYTQVEVNLTTSVTFDGQTYPAGSPLEFSVARNEVVQIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C7N3</accession>
    <name>A0A9Q1C7N3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">G-protein coupled receptor moody</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_13823</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8039728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C7N3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00637">
        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248">
        <property type="entry name" value="ADRENERGIC RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="31"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="65"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="107"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="144"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="189"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="326"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="368"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="10">
        <location>
            <begin position="44"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8039728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="403" mass="45244" checksum="72F40E3691132DB8" modified="2023-09-13" version="1">MACPKQVEWQRSHRYIEFEDYHRPFLFPLEFMSLAIVLVLAVLFNFATIITILRVPSLRQKLGNMLIINLCVMDLITSLGSMLFSFIDIFYEGYFLCAEAFCRIQGGLAVLGCFGNFAAILLIALHQFIGVFAADKTTITSKHIGLMIVGCWVFTLAMVIPSVTGIAATSVYTHGNHHCSPTWVQSCPYYITCVSIIYVITLPSIIICYCTLYWKVKTSREQLVIHEDKVQMDDRNLISKDQGTTSTRTKHDTSTTDQAIINDLSDSSSHLNSEVSMTVVKNSSPYSQASYKKLSAVKKSGSCQSGTSKTKSATSAKKKVTQSHEVHFRIALAGGLLCVTTVVCWTPFFVVYPCIRNEENSHTLSVSAMWLAYTNSVLDPIIYAIFDKKLRKAILNHFHFLWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C8B3</accession>
    <name>A0A9Q1C8B3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_14155</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8039984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C8B3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8039984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="183" mass="20366" checksum="21A8FA3A546D0669" modified="2023-09-13" version="1">MYGTSGIQITIQNQPILKLGKSGKYTYLGHDISLHGKASEAQLSSIAIEFHNTLRKINECPLPVSAKIQVVNDMAISKLNFYFSNITFPAKTLANMEDNIVKYIREWFHLNKSSNRDIIFLPSSKGGLGVISPSSMYVSKKLSFLLQALNSDDPQTKYSARSSFQLHMSKRKAVLHDANEGVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C8D3</accession>
    <name>A0A9Q1C8D3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Cholecystokinin receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_14284</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C8D3"/>
    <dbReference type="OrthoDB" id="2132067at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032870">
        <property type="term" value="P:cellular response to hormone stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24241">
        <property type="entry name" value="NEUROPEPTIDE RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24241:SF76">
        <property type="entry name" value="NEUROPEPTIDE SIFAMIDE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 9" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="25"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="142"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="8">
        <location>
            <begin position="1"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8040088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="262" mass="29857" checksum="DBF2FFAC0F2ED526" modified="2023-09-13" version="1">MKDERTDTMYCAVAWDSNSTAYKLYAAFNLIFLLISPTGMMILAYSSVIYALYKNKKATAGRKQSSPGRLSVTFQNGATKLQLHASDEPSSTSTLLSDVSPAREVRRDQNGNRCNAKNRCSPFRKAVSRVERDEDADKIKRIVVMLIVIVLLFVICWAPVLVLNFIIRFEIIHLDGHTEFILSTTSHLMAFSNSSVNPFAYALLSKNFRESTKQVFRQCCFRTEQRHLQHRPSISSTRFSRLSSSIPRWKGVRNDYVKEDPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C8R9</accession>
    <name>A0A9Q1C8R9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_14549</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C8R9"/>
    <dbReference type="OrthoDB" id="250548at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019351">
        <property type="entry name" value="DUF2039"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22876:SF5">
        <property type="entry name" value="CHROMOSOME 9 OPEN READING FRAME 85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22876">
        <property type="entry name" value="ZGC:101016"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10217">
        <property type="entry name" value="DUF2039"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="137"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="143"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="164"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8040298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="177" mass="20082" checksum="70B4C74F7B56EF43" modified="2023-09-13" version="1">MSLQRGNHKKRGQKYQNTTAFKNDLHDRSGKTKAMNSMVISEVCARCKDVIQWKIQYKKYKPLSQPKKCTKCLQKTVKAAYHVICSKCAKENGVCAKCGGKEDIVHKPVLSPAERASQETRLQGELKVMSERQRRTFYRTLEKDEDADDEEEEGDGDECRSDSEIEEGTDDENGNAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C8Y0</accession>
    <name>A0A9Q1C8Y0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Target of EGR1 protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_14661</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Inhibits cell growth rate and cell cycle. Induces CDKN1A expression as well as TGF-beta expression. Mediates the inhibitory growth effect of EGR1. Involved in the maturation of snRNAs and snRNA 3'-tail processing.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Interacts with U1, U2, U4, U5 and U6 snRNAs.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus speckle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CAF1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C8Y0"/>
    <dbReference type="OrthoDB" id="414075at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015030">
        <property type="term" value="C:Cajal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017069">
        <property type="term" value="F:snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034472">
        <property type="term" value="P:snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000039">
        <property type="entry name" value="Target of EGR1 protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051181">
        <property type="entry name" value="CAF1_poly(A)_ribonucleases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006941">
        <property type="entry name" value="RNase_CAF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15092">
        <property type="entry name" value="POLY A -SPECIFIC RIBONUCLEASE/TARGET OF EGR1, MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15092:SF37">
        <property type="entry name" value="TARGET OF EGR1 PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04857">
        <property type="entry name" value="CAF1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="311"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="469"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="315"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="332"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="474"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00057484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00062362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00071349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ8040388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="488" mass="55677" checksum="15554554EFFB251A" modified="2023-09-13" version="1">MTRFLSVPVVDINRDNFKQFWPSVVFACKTASFIALDAELSGLGPRKQLVAKNLPERYKAICDVAKTRAVLSLGVSCYQYKPPLPDQAVRKKANFAVQTYNITTLCNEDYMVEPAALQFLVEHGFDFNKQYSKGVTYHPGQDKDNDGTEISIRNLFGEILMSGTPVVVHNGLVDMTFLYHHFYTTLPPSYSQFVGDLSEMYQGGIFDTKYLAEFVVRMPSSYLEYVFRKCQRDNLKSEEEGGKFFTLTFQEYLEGSFVEYHPCFLPERKISDPSSEKKICQLYWAHGFCNQATCDGTHNLDIILDADLEQEEQKKRRRRRKRKRKKRGQSEMVDESEGDTEMLEETEQDISVASPEKISIEKEELSGEGRNVEGSIICKDNGIDEAIKESKREQINGKLPRQHGSHRAGFDAFMTGFAMACVISKRGHFPSNEQTEKKHLAMFGLNGEANKLFLSGKNIPMLVTKSSFAKPSQAHKEKMKRLMEQHSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C983</accession>
    <name>A0A9Q1C983_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Hippocampus abundant transcript 1 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_15051</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C983"/>
    <dbReference type="OrthoDB" id="419616at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17387">
        <property type="entry name" value="MFS_MFSD14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001958">
        <property type="entry name" value="Tet-R_TetA/multi-R_MdtG-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23504:SF1">
        <property type="entry name" value="GH21943P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23504">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01035">
        <property type="entry name" value="TCRTETA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="60"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="93"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="114"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="148"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="178"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="233"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="271"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="368"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="417"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="25"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="441"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="469"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8040687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="482" mass="53110" checksum="2C5BC07038C2756B" modified="2023-09-13" version="1">MKKRKKIPRKKAKESIELTGIGDPSIYHALIVIFLEFFAWGLLTNPMLTVLNETFPKHTFLMNGLVQGVKGTLSFLSAPLVGALSDVWGRKSFLLLTVFFTCMPIPLMKITPWWFFAMISVSGVFSVTFSIIFAYVADITDESERSSAYGLVSATFAASLIISPALGTYLGSIYGDSFVIFLATAVALCDILFVLVAVPESLSEKLRPASWGAAISWEQADPFMVLRKVGQDYTVLMLCVAVFLSYLPEAGQYSCMFLYLKMVGNFSEPEVPAYIAFVGILSVFAQTTILGILTQNFGHKHTIMVGLTCEMLEQMWYGLGSELWMLWSAGVLVAISSINYPAISALVSRNAPPDKQGVLQGMITGIRSLCNGLGPALYGFIFYFFDMDLDQYNKDEPPAKVDPGSSLQVFNLPGPPFVFGSVLVLLSQLVMFFLPRDDEHDSTKPMSPRSFQMQPVSPGPDLVVPLLEDSNKPVHSEDFNNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C9G8</accession>
    <name>A0A9Q1C9G8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cholesterol side-chain cleavage enzyme, mitochondrial</fullName>
            <ecNumber evidence="5">1.14.15.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">CYPXIA1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">Cholesterol desmolase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">Cytochrome P450 11A1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="24">Cytochrome P450(scc)</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="ORF">HOLleu_11917</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="27">Nanhai2018</strain>
            <tissue evidence="27">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 25">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism; C21-steroid hormone metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 26">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="27">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.15.6" evidence="5"/>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C9G8"/>
    <dbReference type="OrthoDB" id="3945418at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008386">
        <property type="term" value="F:cholesterol monooxygenase (side-chain-cleaving) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006700">
        <property type="term" value="P:C21-steroid hormone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071375">
        <property type="term" value="P:cellular response to peptide hormone stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034650">
        <property type="term" value="P:cortisol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006704">
        <property type="term" value="P:glucocorticoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11054">
        <property type="entry name" value="CYP24A1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000006">
        <property type="entry name" value="Cytochrome P450 302a1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050479">
        <property type="entry name" value="CYP11_CYP27_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24279:SF3">
        <property type="entry name" value="CHOLESTEROL SIDE-CHAIN CLEAVAGE ENZYME, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24279">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0153">Cholesterol metabolism</keyword>
    <keyword evidence="8 25 26" id="KW-0349">Heme</keyword>
    <keyword evidence="13 25 26" id="KW-0408">Iron</keyword>
    <keyword evidence="15" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 25 26" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14 26" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="12 26" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="19" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="20" id="KW-0755">Steroidogenesis</keyword>
    <keyword evidence="18" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="11" id="KW-0809">Transit peptide</keyword>
    <feature type="binding site" description="axial binding residue" evidence="25">
        <location>
            <position position="480"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00019844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00030343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00032666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00033274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00033394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="EMBL" id="KAJ8041177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="532" mass="60954" checksum="DC969D52E25C05AB" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CC50</accession>
    <name>A0A9Q1CC50_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Reverse transcriptase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_12927</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09274">
        <property type="entry name" value="RNase_HI_RT_Ty3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000115">
        <property type="entry name" value="Polyprotein of retroviral origin, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.370:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 17.6-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000032">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 297-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon gypsy-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054465">
        <property type="entry name" value="Integrase_p58-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041373">
        <property type="entry name" value="RT_RNaseH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22938">
        <property type="entry name" value="Integrase_p58_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17917">
        <property type="entry name" value="RT_RNaseH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="9">
        <location>
            <begin position="752"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase" evidence="8">
        <location>
            <begin position="1166"/>
            <end position="1343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="221"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="485"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="585"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="221"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="236"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="260"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="485"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="529"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="554"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="601"/>
            <end position="625"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8041975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1558" mass="177547" checksum="CC447F35D67C2C51" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CD01</accession>
    <name>A0A9Q1CD01_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Synaptic plasticity regulator PANTS</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Plasticity-associated neural transcript short</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_10352</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Synaptic cleft</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0545 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CD01"/>
    <dbReference type="OrthoDB" id="5946508at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043083">
        <property type="term" value="C:synaptic cleft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021475">
        <property type="entry name" value="Pants/Emi1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28052">
        <property type="entry name" value="UPF0545 PROTEIN C22ORF39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28052:SF1">
        <property type="entry name" value="UPF0545 PROTEIN C22ORF39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11326">
        <property type="entry name" value="PANTS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="62"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00043942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00044072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00044235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8043323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="95" mass="11223" checksum="FF0795E4D8A45D19" modified="2023-09-13" version="1">MTCWKDCKSWRNMFHEYYVHGRQPSCDQWKEDYNNCILWKTEQSGPAKEALMQSEQLRLKKVNDDSPPVWKLRTAPCPDWPKNPSSGTIPHEEPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CD04</accession>
    <name>A0A9Q1CD04_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_09506</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8042685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CD04"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47510:SF3">
        <property type="entry name" value="ENDO_EXONUCLEASE_PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47510">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8042685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="222" mass="25762" checksum="220056A88CA4814F" modified="2023-09-13" version="1">MGCRKYVDFDTYPQSNSKLDAVLARGEYHKVKPAPPISGSDHTIVHVLPVLTEKHRESQPKKKKLKPDPSKDNTDILRDALVTTDWNVFRNPRLNIDELTEVASCYINFVSDLHVPKKATPLVTLSKRIPADVDIKRLEKEKRHAIEVKDRELRNRIQRDINRRVYQKRTSVFQEVVTADSKSLWDLLRTLRSPDNDEKQPVSKEMANELKVYFGRFNDSIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CGW2</accession>
    <name>A0A9Q1CGW2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_08023</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CGW2"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8045092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="82" mass="8898" checksum="4D89B47FF15C98D7" modified="2023-09-13" version="1">MKVTKESQFQRKWSHDSTPCQLVSQLLCSPLTCISLLHNGLATASPIAVSYQQISNCTLMSSCPLFVTSQLTSASSPLDCSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CHW9</accession>
    <name>A0A9Q1CHW9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Bcl-2-like protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_07810</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Bcl-2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CHW9"/>
    <dbReference type="OrthoDB" id="6021377at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051400">
        <property type="term" value="F:BH domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097192">
        <property type="term" value="P:extrinsic apoptotic signaling pathway in absence of ligand"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008630">
        <property type="term" value="P:intrinsic apoptotic signaling pathway in response to DNA damage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001836">
        <property type="term" value="P:release of cytochrome c from mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01670">
        <property type="entry name" value="Death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.437.10">
        <property type="entry name" value="Blc2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036834">
        <property type="entry name" value="Bcl-2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046371">
        <property type="entry name" value="Bcl-2_BH1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026298">
        <property type="entry name" value="Bcl-2_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002475">
        <property type="entry name" value="Bcl2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000488">
        <property type="entry name" value="Death_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11256:SF62">
        <property type="entry name" value="BCL-2 BCL-2 HOMOLOGY REGION 1-3 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11256">
        <property type="entry name" value="BCL-2 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00452">
        <property type="entry name" value="Bcl-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00531">
        <property type="entry name" value="Death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56854">
        <property type="entry name" value="Bcl-2 inhibitors of programmed cell death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50062">
        <property type="entry name" value="BCL2_FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50017">
        <property type="entry name" value="DEATH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Death" evidence="3">
        <location>
            <begin position="81"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8044919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="280" mass="32426" checksum="D85DBAA33039E402" modified="2023-09-13" version="1">MMTGGLTTKESKDYIIDYCLYRIRHHKRNSPNNEHLMSDPWEILSQQLAAEDHDSGIIPEKDLWIHFQTISLDIGRQGEFLGRSLGLPESEISIIQEDYSRNCREKNYQILKKWKELNGREATYECLSAAFLDVGRKDLEEKYCKSTEKERKNSIGHLFNVNAKTAYAQFKDLADELFRDLEEGASWGRVAAAISHPWRIALHFATDSVEDLAPDVREWTSRYLDKHLSNWKEEHGGLDGFMDFHAKDKARECFQGTMCLLGPCCLYCWLESNDMCSKDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CJZ4</accession>
    <name>A0A9Q1CJZ4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_04981</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CJZ4"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF13">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="78"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8046341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="235" mass="27705" checksum="85B9574449161F04" modified="2023-09-13" version="1">MPFNLDKFVASPSVEELDSLKKSEIVKVAKHYGIEFQPLMGKDEIKRYVLEYLVDESILPSTVLETIITVPTDNTFELKRLEMEMNKEVRLKELEKEEREMQMQREEKAREHEFRLKQLELGVIKGSDPKIGLDTGGFDISKHVKFVPKFQENNVEKFFNHFEKLGEQLKWPRDKWSILIQSNFTGKAQEVYSALSIEDSMDYDKVKKAILQAYELVPEAYRQKFRKYRKADTQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CK73</accession>
    <name>A0A9Q1CK73_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_04832</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CK73"/>
    <dbReference type="OrthoDB" id="10541492at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050934">
        <property type="entry name" value="ITIH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10338">
        <property type="entry name" value="INTER-ALPHA-TRYPSIN INHIBITOR HEAVY CHAIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10338:SF108">
        <property type="entry name" value="INTER-ALPHA-TRYPSIN INHIBITOR HEAVY CHAIN H4-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040367597" evidence="2">
        <location>
            <begin position="26"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="345"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="398"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8046220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="486" mass="55654" checksum="81195593F5964860" modified="2023-09-13" version="1">MENFVSKGILLLFLHFNLSIGTTSTGSFDTWSWVALNPGRDRVAEWVSPSNTQEIDINKLHVFSNISELHSDVYVSMRFRNPHQLRLYLHFTLRMGDVSRVNGFKIDHMGSKFKAERVHQARAERLLERQRNDGFTSSGIIMIRTSPYLREFDVHVFLESRTSMTFDLNYKEKLQSLLSANWYEQQIRIYPYQLVEDFEMNVVIQRPRIGTLSDVDAYWDTFSASNTVQDPWGLQREPLTVRPWNGGSRINLSPVRSDMTNPRSWQSDLTVIFEWDSPEFGFDENSWTSHSDAAIHFESSDSYSSSVDIIIPGVTHEPHITDPVLTISTESRITSNMSLEEKYVSGSGWVGSGDDREDFITEKGSGVDLETDPPFIKDDTETDSFIQQSDTSSDEGDSSSSSLSSSSSEDSSIEHSSSDFDFTEDPTPQINIEKFIEKNHVHFKIYQKETSLEFKVPSSGMNSKVVSMFKRIMKKFFPKRKETSED</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CKS3</accession>
    <name>A0A9Q1CKS3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_06338</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CKS3"/>
    <dbReference type="OrthoDB" id="416119at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19446">
        <property type="entry name" value="REVERSE TRANSCRIPTASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="127"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8047357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="397" mass="45685" checksum="F0C0C04FB532F0B2" modified="2023-09-13" version="1">MFSHDLVDIWRERNPFDKNFTWSSNVTPGIHCRLDYFLISRHASPAVTDNVFSPGFLSDHSFVCLSIGTQEACRGPGFWKSNNSLLNDSKYICLIHELICNQQKENASYDPVFRWELLKFNIRKTSMKFAKERAKEMRLKEKKLIQKIADLEQQFYVNRSPVIHTQLREAQNDLLLYYEYKLKGTMIRSRARWVEDGEKNSKYFFNLEKRNKALNSILKLEKSNGIFLTERNGILEEIRNFYKTLYTSKGCNPESFFDGLPDQNIADIDLESCEGELTLDECYQALVSMKNDKAPGSDGLSVNFYKTFWNSIGQVVLESLNFGYQRGQLSTEQTRGIVTLILKGNKAATSLQNYRPITLLNTDYKIAAKVIASRLKGVIHSLIGPHQTGFLKGFGIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CLA2</accession>
    <name>A0A9Q1CLA2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Solute carrier family 35 member G1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_05869</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CLA2"/>
    <dbReference type="OrthoDB" id="306876at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22911">
        <property type="entry name" value="ACYL-MALONYL CONDENSING ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22911:SF6">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER G1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="26"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="83"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="138"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="172"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="204"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="238"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="266"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="292"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="6">
        <location>
            <begin position="24"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="6">
        <location>
            <begin position="174"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8047001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="321" mass="35164" checksum="930050E66B1CBB78" modified="2023-09-13" version="1">MPNQKHGTDPSWASHLCRSIYSQRGILFAFVGGILWSVNGLAANIITKTVGVWVIGYFCSICQLLLIPFLQWDYAERYGVAEVSIIILSGLCDTIVLFLACYAYSLTTVGNTGALLYSKPLFCVILSRLVLQVKYSVYDVVILVCNLTGIALISKPPFLFASLTDDEIITEFYGSMVAIGAAVTGAFQFIAIRKLVERDACDRFLLLLVKGIIGFFLCSMVLATNLSHGGHIKSIYDLLWLTLQCGSGLCSCLFVYAALQSQDASTVALVSTVEVLASFLLQVAFTDDSVDWMDIVGAFMITICPVWYAVKGILCEYFDSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CMW0</accession>
    <name>A0A9Q1CMW0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_06957</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CMW0"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8047845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="112" mass="13023" checksum="19953D30B128AE47" modified="2023-09-13" version="1">MPSREVHKDTSEANQVINGGDLPELATAQMGPPQADQHPKFPEQQQQPVQNGVNKRQELCSEQAQSTMRYPTRQRQPRHVFTYDQLGEPRLYQSRVNSMHYAQWNRMLPHTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CNP1</accession>
    <name>A0A9Q1CNP1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_00488</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CNP1"/>
    <dbReference type="OrthoDB" id="5954038at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37162:SF11">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37162">
        <property type="entry name" value="HAT FAMILY DIMERISATION DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8048246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="338" mass="38471" checksum="0CAAEBEF63BCDD0F" modified="2023-09-13" version="1">MGESALTSHLKGKKHADNCKASGRQSTNIRDFMKPNQGKSTAGAEAAVGSSTTRSSTLETFVTREQTLKAEILWSLKVIDSHYSYRSCAGNDKLFQEMFPDSQIAKEFTCGEKKCAYLVCFGLAPHFKQMLIDSMKEEENYIIMFDESLNAVTNSKQMDIHVRAWNRCSNQVESRYYTSVFMGHGTADDMLIHFHEGMQGLHLQKIFQVSMDGPNVNWKFYRTLCDEIKKDFACQTFNIGSCGLHILHGGFKDGASASGWQIEKLFSSLHWLFHDTPARREDFTKVTGSSVFPLQFCKHRWLENLPVAERVQSMWENILKYVETSYSKEDTLPSDNVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CP72</accession>
    <name>A0A9Q1CP72_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_02107</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CP72"/>
    <dbReference type="OrthoDB" id="10063305at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8049382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="137" mass="15911" checksum="C36180AFE5CAB125" modified="2023-09-13" version="1">MSKTVANALTMVNKPEYESTIYFITMVNKFFDCMNVTTVMEWERKRNDDLPPYSDANDIRFKWLKEGFLDKWYKDVEQPGLTAKQEACRLSRPTMAGCHLIVNTFVDVATYLLSKDGVKYILSGKFNQDPVEELFSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CTM9</accession>
    <name>A0A9Q1CTM9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_03607</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CTM9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8050409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="83" mass="9936" checksum="9F4CD75137CD32B6" modified="2023-09-13" version="1">MYKLFFRNTNLRVEPIVFNRVKELGIRKTFRGQRGRPASIKMSMNWKANWDYNNTVHRELLRSLPNEHIYNNNNNCKLGLINA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CU72</accession>
    <name>A0A9Q1CU72_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_04176</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CU72"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF13">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="78"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8050829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="220" mass="25957" checksum="4DBDED58829790EF" modified="2023-09-13" version="1">MPFNLDKFVTSPSVEELDSLKKSEIVKVAKHYGIEFQPLMRKEEIKRYVLEYLVDESILPSTGLETAITVPTDNTFELKRLEMEMNKEIRLKEMEREREREEKAREHEFRLKQLELGVIKGSDPKIGLDTGVFDVSKHVKFVPKFQEDNVEKFFNHFEKLGEQLKWPRDKWSILIQSNFTGKAQEVYSALSIEDSMDYDKVKKAILQAYELVPEAYRQKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CUP2</accession>
    <name>A0A9Q1CUP2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_04651</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8051184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CUP2"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8051184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="56" mass="6530" checksum="C5E179F6EA56083D" modified="2023-09-13" version="1">MMLKRESAKSVGMNKFTKALARNNTPRELIGSPDRWNDLSSWRDLDDAPGRVRERR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1GYY8</accession>
    <name>A0A9Q1GYY8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_30025</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8027928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1GYY8"/>
    <dbReference type="OrthoDB" id="5986462at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46704:SF9">
        <property type="entry name" value="BHLH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46704">
        <property type="entry name" value="CXC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8027928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="309" mass="33907" checksum="F4910955D9BD8279" modified="2023-09-13" version="1">MFSFLASKVADIDTNKHLITTKGTGVLCSNRQDVSALAPCTHEEADTRILLHPQDAVQQGYRKVSIRTVDTDVVVLAIASANRLNISELWIAFGVGKSFRFIAAHEIAKALGPDRRVALPMFHAFTGCDTVSCFGGRGKKTAWGTWITNGDVTPAFCALSAMPDPRATDEWMQPLERFVVLLYDHTSTEEGVNQARKQLFSKKGRAIDGLLPTQAALIQHTKRAAYQAGHCWAQMMTPAPELPSPSEWGWNKKPGGGWSITWMTLPEASEVCRELLRCGCKNGCRGRCKCQKAVLQCTALCQCGGQCYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1GZL4</accession>
    <name>A0A9Q1GZL4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Solute carrier family 25 member 43</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_30447</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1GZL4"/>
    <dbReference type="OrthoDB" id="270584at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24089">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="106"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="161"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="204"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="7">
        <location>
            <begin position="10"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="7">
        <location>
            <begin position="103"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="7">
        <location>
            <begin position="198"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8028258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="329" mass="36823" checksum="07FAEAEF60B190D3" modified="2023-09-13" version="1">MAATRRDNRLTFLQNFTCTAAAGIAARTVISPLDVTKVLMQVGTKDTHEGLLKTFTNVYKSQGIRAFWKGNFMGCLRLVPFSAIQFSAFNQLKRNVVDDNGRMNSIHAMMVGCMAGMVATVVTYPTDMVKTRLIVQSKQRRRYRGIAHAFALIMKEEGLLAFYRGMLVSLLGSMPFAAITFSSYETLDQIWGKPRYMYTPMQSLLNGCISAAVAQSLCFPFDTIRKKLQAQSPVIRDGGGVDVQFKGAIGAVRATFRRYGFAGFWRGNAANLCKIVPYSGIMFMTFEALKRSMLYENGYTVSMFDDTPKQGIDQTMQPSELVAWRKRHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H1U0</accession>
    <name>A0A9Q1H1U0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_28213</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8028951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H1U0"/>
    <dbReference type="OrthoDB" id="77878at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017171">
        <property type="term" value="F:serine hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008547">
        <property type="entry name" value="DUF829_TMEM53"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20908">
        <property type="entry name" value="LD15586P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20908:SF4">
        <property type="entry name" value="SI:DKEY-5I3.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05705">
        <property type="entry name" value="DUF829"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8028951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="174" mass="20046" checksum="9AD6C597309BDA2D" modified="2023-09-13" version="1">MSIGAYIYSIILIEALKNQEAVSYLNQRVKGNIFDSLVCGGLNQMAVGVSKLIFKNFILEKVFTTTLLSYFTLTKPYTVQEYDKITQQFIDNPFTPSALIFYSTVDPMSDPNVVEYTGQTWGKRSAHIQMKKWDDAGHVELFKKHPKEYVERLDEYIDFIRPSVRPYKPVVAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H1V3</accession>
    <name>A0A9Q1H1V3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_28954</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H1V3"/>
    <dbReference type="OrthoDB" id="88467at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040293155" evidence="1">
        <location>
            <begin position="19"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8029535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="354" mass="39151" checksum="06020D63F193637B" modified="2023-09-13" version="1">MNLLLVVFLAIVPGSVIGFRHTGKVVTVDSFMWNLQEPSKIKIDAKSPITIDFVAQIAPQSDSIKGKNLWRLGIFGSEHNDGHGERVGFKSQILKAKQASKRPAKGQPLKMKNIFSRFDLSQIGCNEVRYFCAEFAKNSDSTVDFELRSGEALDNIVSCKEMECRRPNADSTTLEGRETLPELLIPPPTARLDSLDMTLAIRGVCFDGSHNISMDAQVDPHHSTDTIRGRGLWRLGVFLSNASYGSMARSQYQPDLLNVTHKAKPLISGETLVFTDLHTALNTSGVGCREAYLQYLCIEFDRGENPIPSFELPEGPAILCSRVVCTWPGQSRELPEHSLDKALLTGYPKLLGQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H1Y8</accession>
    <name>A0A9Q1H1Y8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Gonadotropin-releasing hormone receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_29067</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H1Y8"/>
    <dbReference type="OrthoDB" id="6435638at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45695:SF22">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45695">
        <property type="entry name" value="LEUCOKININ RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="64"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="101"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="143"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="10">
        <location>
            <begin position="43"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8029625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="164" mass="18529" checksum="4524FEE3B4394228" modified="2023-09-13" version="1">MDGNISSSPAVNTTNFTDLWEHDIGHTVRGSVLIGIFVTSVFLNCFLGLILWRDRKHFSRINLVVFHLMISDLSITFFNIPVDFIWIQTKAFLAGTQVCKVIMFLRQTTLFSSSFVVIVISLDRCMAIVHPLSVRQADFRCRIMLTVAWVASIICSIPQVCSTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H2U1</accession>
    <name>A0A9Q1H2U1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_26429</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H2U1"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="124"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="20"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8030121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="272" mass="31312" checksum="80AD43766E983D2C" modified="2023-09-13" version="1">MNHEENERTKKRLGMANIDVEENRTQVQSIQQELQRIKQEYKETEKLLQQTKDLHQQTALVRNKFKAECDQYKLELSSLEKNLETAEQDLLQTKDLLQKATQEKNIIRAELEQSQLQYSITSMSLEGSPGGNRFSEAAQTRPRDARPRSDGSVSSATSRYLSESALSREFSVLLVKVSVKLTSGHCVLLATYFDLPRSKLDRIRKDSDTPGITLLDVMKERSIINMYDVSNLQQALADIDLHDINQTLVKPYQEKIDPLMYERHKVGQQDKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H3E8</accession>
    <name>A0A9Q1H3E8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Alcohol dehydrogenase [NADP(+)] A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_24705</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldo/keto reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H3E8"/>
    <dbReference type="OrthoDB" id="416253at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000006">
        <property type="entry name" value="Aldo-keto reductase family 1 member A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11732">
        <property type="entry name" value="ALDO/KETO REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00798">
        <property type="entry name" value="ALDOKETO_REDUCTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="7">
        <location>
            <begin position="17"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="4">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="6">
        <location>
            <position position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8031498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="316" mass="35713" checksum="358D933F23D1651D" modified="2023-09-13" version="1">MANLYATLSNGGKIPLIGLGTWQKSAARDVELAVKAAIDTGYRHIDCAYLYQNEKEIGDALKEIFQAEKVKREDLYITSKLWGTFHRPDKVEHAIRLCLKNLQLDYLDLYLIHSPMAAYYKSDSELLPTKEDGKPYFEDVKLADTWRAMETLVQKGLTKSIGVSNFTVSQLNEILPTAKVPCVMNQIENHPFLDQKELINFCKSKNIGVTAYCPLVSPCSPWVTDKDPKHLQDPVVMILADSKGCTAAQVLIAYNVSQCIVCVPKSVNSSRIKENFKSYDVELRDEEIQKLDALASGFKACTFDFWKDHANYPFQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H4J5</accession>
    <name>A0A9Q1H4J5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Tumor necrosis factor-inducible protein6 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_25470</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H4J5"/>
    <dbReference type="OrthoDB" id="10009301at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24251">
        <property type="entry name" value="OVOCHYMASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="15"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="146"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="5">
        <location>
            <begin position="31"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8032056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="308" mass="34518" checksum="133003DD0101B05F" modified="2023-09-13" version="1">MMYQRQHRGSLQRRISLFMVANLCLYAGLTTGNSIVVLNKPSGLFQSPGYPDLDDDMHLIWKVIAPLHHKIILTFLDFDLVEMAFCIDEIEIYEGNNLKGTYRGTQLHQKSITSTSNTISIVLMSCPTSLWNDRKGIRVSFSTGSLYAIIGAVLALLFVLMIIAVLFIRKRREARFLTSDSATHAETSGKNQENGYFTTVASERTTVKSLGHDYEEANETQKDMSGVYYSRGIVSKSWGSSRPQNKHLKFDAGSKLGVGLYENNISNTRNKTVDDQDYSHEICRSQQASLTVAYVNNLKRSIEMSGGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H6A4</accession>
    <name>A0A9Q1H6A4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Fas-binding factor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_17900</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H6A4"/>
    <dbReference type="OrthoDB" id="8195456at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097539">
        <property type="term" value="C:ciliary transition fiber"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090162">
        <property type="term" value="P:establishment of epithelial cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033561">
        <property type="entry name" value="FBF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33689">
        <property type="entry name" value="FAS-BINDING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33689:SF1">
        <property type="entry name" value="FAS-BINDING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="257"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="140"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="177"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="257"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="328"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="361"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8037152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="457" mass="49386" checksum="1DB4E3BEDD42B1B6" modified="2023-09-13" version="1">MDDMDDALFASSLRSRKSSTLKSDKSVEAAKNSGNDRELAGTRTLSPPSLVNTADSKRRTFTIKEEGKASEKSQVAGKKPLTKSFDFGEFDEDDPLAGLLSDEDEEPAPKQKGLQRTARPQPKVDVNKPVEDADVDSPRKSSPSMKRSTSLNKGGAGEKEIEVEQSITRAKSAPPPKRSETPKRSDDIDFGDDDILDTMGFDDSPPQTKNAPEKKKEDDEILVAKSKVADLFGKKPAISPPDKPPVGRTKEFVLDAKYRKQERGNPQKELDEEDFSFGSYTPSAVNTLKRPDSAPPGRRSVRFADDVDDLFGGTSTLGRPGAGGGGRQRSNSQVVGPSDGNKSTGQGDDEGDWLSLAIGKSNERKAAEKKETLGVTGNKEETVGQETEQKNETKNTVTDQRKKGILREEKTKGPKEAENPRAASAGDYLGLGEDIDLDTLLSLVLFSSSLRGGGVEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H6X0</accession>
    <name>A0A9Q1H6X0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_18719</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the IFIT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H6X0"/>
    <dbReference type="OrthoDB" id="10043504at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10271">
        <property type="entry name" value="INTERFERON-INDUCED PROTEIN WITH TETRATRICOPEPTIDE REPEATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10271:SF0">
        <property type="entry name" value="INTERFERON-INDUCED PROTEIN WITH TETRATRICOPEPTIDE REPEATS 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8037812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="472" mass="54487" checksum="C8027CBCA4B21DB2" modified="2023-09-13" version="1">MTSETSDWWKRVPCHFNWDLEDDVTVDFTSVVNNLDSKLESLKESNGGNSSCVLLLFRGYLEVSKFAGVTSNPEKANAFFDQADSEAEVLIDDEERRAYVVVSSANRLWVLEKFKIGSESEMRRKKEYLVSKLEENWQSGVGRKSGNFDAYLEAVAAFALTRLGPYKYREAESRYRKAIKLNSKQAEWFHALGELVGRQARQKTQSSGECYSDMDEEIRCYQKALDLDPENDFARCDLALVLARKDDKLQDAKDLIALTKDVACEVIIKKGRFYRRQKDFQKALAVLKKGTKLQNPRSELFFQISLVCSSLASQAGYKKNYKEKTEYLEKEMEYLDKCIQREPSHFFAKLNKARNFSKSRRNEEAEQYYRKILNECHSSPRNLIEAQFTFAEFLALSNRGRIPQEAADLYEEMVNTGAVILREIDQDGNAKVTGKDGFLEKARDRLKDFYLEREEGSRKMKELEEKLSTLGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H8A7</accession>
    <name>A0A9Q1H8A7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_20175</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H8A7"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8036255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="70" mass="7510" checksum="47138D559B06A0DE" modified="2023-09-13" version="1">MVLSAIGKIESEWLAMLTSGGKNVLLPNQLYTLCKGNLPSRQHDVSTLVSGLCAGAAVRTNWLKKSACYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HA24</accession>
    <name>A0A9Q1HA24_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Macrophage mannose receptor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_12354</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HA24"/>
    <dbReference type="OrthoDB" id="6369810at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050111">
        <property type="entry name" value="C-type_lectin/snaclec_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22803">
        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0675">Receptor</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C-type lectin" evidence="2">
        <location>
            <begin position="17"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="2">
        <location>
            <begin position="148"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="2">
        <location>
            <begin position="276"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="2">
        <location>
            <begin position="405"/>
            <end position="519"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8041517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="523" mass="60402" checksum="FAA1022EBDF26BFA" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HAX6</accession>
    <name>A0A9Q1HAX6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_17092</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HAX6"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8039389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="61" mass="6933" checksum="8EE6DB3991A628D5" modified="2023-09-13" version="1">MRSSKRKRNEDSGGPSNKKVSGKTLICLFQPGQTIGTITREMQFVRNLKAWKAILQTERPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HBX6</accession>
    <name>A0A9Q1HBX6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_14675</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HBX6"/>
    <dbReference type="OrthoDB" id="6162224at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8040395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="132" mass="15700" checksum="0CC37798C597BAF0" modified="2023-09-13" version="1">MATRKKHVSSPPRIKTKFSASPSIERSDFENFFFEKGISSLIHFFPKNFNLEKFRALTEDDLQDDYRVEDAEDRQRLMRAINLCREEEDDRTEGQLDNIGDTEESLEGEIFTKANPFGCYRRELLLLYDHRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HFG3</accession>
    <name>A0A9Q1HFG3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_10068</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HFG3"/>
    <dbReference type="OrthoDB" id="5981530at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008528">
        <property type="term" value="F:G protein-coupled peptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009755">
        <property type="term" value="P:hormone-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24372:SF77">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24372">
        <property type="entry name" value="GLYCOPROTEIN HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="51"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="97"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="136"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="220"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="262"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="298"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="9">
        <location>
            <begin position="21"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8043108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="334" mass="37029" checksum="79A4AC3E48234D98" modified="2023-09-13" version="1">MLPNTILRITLWVVCLFSLLTNLFVIASRVQSLIPVIGNMFSSAVSDKQNAFLINLAVADFLTGLYLLAIGIADAIFGKAYFLSALGWRKGLICKGIGFVGFLANIASILTLTFVSVERFFVIVFPFSKIRFRSKLTVTICVTIWAISGAMAITPIVLSEFVEKVFGFSDICLGLPFVYVTEISKYPSLNMEYNFNGVVLQTVYYNNDVQGVQWLYSQIVYVYFSATCVLVITICYIAIIISTIATKRQSGRPGNNKNELKLALRVAIIVGTDLLCWFPVILIGLLSQIGTIVSTDTYAWFAVFVMPINSAFNPFIYTIPAMQSKKRNETSFAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HFH5</accession>
    <name>A0A9Q1HFH5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NIF3-like protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_10101</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GTP cyclohydrolase I type 2/NIF3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HFH5"/>
    <dbReference type="OrthoDB" id="3345469at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1390.30:FF:000001">
        <property type="entry name" value="GTP cyclohydrolase 1 type 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1390.30">
        <property type="entry name" value="NIF3 (NGG1p interacting factor 3)-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002678">
        <property type="entry name" value="DUF34/NIF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036069">
        <property type="entry name" value="DUF34/NIF3_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00486">
        <property type="entry name" value="YbgI_SA1388"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13799">
        <property type="entry name" value="NGG1 INTERACTING FACTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13799:SF13">
        <property type="entry name" value="NIF3-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01784">
        <property type="entry name" value="DUF34_NIF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102705">
        <property type="entry name" value="NIF3 (NGG1p interacting factor 3)-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="108"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="125"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="454"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="458"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR602678-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8043138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="493" mass="54020" checksum="3E45AF825B423F8E" modified="2023-09-13" version="1">MLAVNASVLTKTLLRLEVAFSWGNCSLPLRLSTTLIHREPNSSLSHPSTWQPNVRHVRSLTCSSVVCLTSGGHRHKSRRYDFKDANSVNFREINSCCNRSVTGTRPMYSSISNLTKAGRKKSKKMSTSPDSKSPSEPEIAEENSPSVASNPELALDKIIEKLEEFAPSSLAEEWDNVGLLVEPSPPHEVHKVFLTNDLTEEILEEAIKLESGLIISYHPPIFAPLKRLTMKTEKQRIIVRAIEKGVAIYSPHTAHDSVKGGVNDWLLSAFGNQTGTAISCSGEDKKYKLEIIVSKQFKQKVQEVFNTENGGHPKCEKKADGSYHFNVICDKETLKKLSTALSAKKVERNTQITKLYPDPCSETGAGRFLELAESVTVEDCVQKVKGHLELEHVQLALGKGKTMESSIQSVAVCAGSGGKVLAGVKADLYLTGEMSHHEILAAVSMGTSVITCNHSNTERGFLKNSFKNTLEQLLGENVAVLVSEVDADPLKVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HFU9</accession>
    <name>A0A9Q1HFU9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_07780</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HFU9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8044894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="187" mass="21606" checksum="1E203AAD743F755E" modified="2023-09-13" version="1">MHPEAKIPFKKRFTSRVSVMTDSAKEELEDFGCLELVKVAVSYKFMHHTCSNMDRQITLKVTIVYFLSFFLSQKTFSMALTCCSDNPMSSQMSSQSLLKDFGKVASIYWVNYGPLKASCLSNAISSNRVYNTRTDNDDAYFLWKIPNAEKSEDNFRGDQMKTLPSVLPRRKRLRENDSIPSKRCRRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HGW9</accession>
    <name>A0A9Q1HGW9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Adipocyte plasma membrane-associated protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_08375</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the strictosidine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HGW9"/>
    <dbReference type="OrthoDB" id="5307922at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018119">
        <property type="entry name" value="Strictosidine_synth_cons-reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10426:SF88">
        <property type="entry name" value="ADIPOCYTE PLASMA MEMBRANE-ASSOCIATED PROTEIN HEMOMUCIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10426">
        <property type="entry name" value="STRICTOSIDINE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20067">
        <property type="entry name" value="SSL_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03088">
        <property type="entry name" value="Str_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63829">
        <property type="entry name" value="Calcium-dependent phosphotriesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="49"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="Strictosidine synthase conserved region" evidence="6">
        <location>
            <begin position="212"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8045375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="435" mass="49305" checksum="0CA53F0BD8C89647" modified="2023-09-13" version="1">MSEKSSIRQRKGREEVKSQQASSSHPADKPWDQRLKKKTPTCWEQCGKYLWMLLLFEFILLLVGIILVLSVESPIKPTRLQFPTARPLEGALAPNTILQNAKRLFEGELIGPESLVMLDGKIYTGTYDGKIVRIDDDKMMTTIARLGREPCGSPEYEHTCGRPLGIHVSDYGTLYVIDAYLGLFEVNVTGEYKQLVSSSRSYHGYPIKFGNDVTRIPDGDIFFTDSDYQWQRRDFPYIMMESSPNGRLLQFNPLSQTTSVTLLDLYFANGILLSPDNDFLLICESTAYRIMKYYIRGQKAGDKEIFVDNLPGIPDNIRPSSSGGYWVGIHFTHLRTGSVPILNFLAERPMLRKQLMKIMKPHFLLQFLPKYGLVIELNKDGEIINSLHDPTGEVIDSVSEVLDTGDALYLGSFKSPFLAKLDYKSKPGKDKFIKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HI48</accession>
    <name>A0A9Q1HI48_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Zinc finger CCHC domain-containing protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_08739</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HI48"/>
    <dbReference type="OrthoDB" id="10064617at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002218">
        <property type="term" value="P:activation of innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042509">
        <property type="entry name" value="ZCCHC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22639">
        <property type="entry name" value="GAG-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22639:SF3">
        <property type="entry name" value="ZINC FINGER CCHC DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="4">
        <location>
            <begin position="29"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="4">
        <location>
            <begin position="46"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="4">
        <location>
            <begin position="66"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="283"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="123"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="217"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="180"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="217"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="247"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8045688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="360" mass="40334" checksum="9BCF328C06314D1D" modified="2023-09-13" version="1">MKLNSDIPSSVSFGGRSCWVRYRGQPRTCLKCGEAGHEAKECGVIRCFNCQSLGHASKECEVPTTCTICGKSGHHYRDCPVSFANKIKPNSSWIIGSHAKTSEGGEKEAEANLGEEVVEVSSDDATHSAIASNPGPSFMADSQPPNEVVSDKQTNDSQILLFEDSEPVVIPDTQASSDVVTDKKSSESEMDISDGRPEIKTLENCIPQPQLQDNETCSNEIQSQPAMSVDKDPIFVKPRRKASSQHDTGRRPERSRSGIRFPLENPMDVIKMTQIFLEDEPWHSCYVKTCCETFSKFNDLKAHVGEHHSKLKPQEYSCARRSCHLTFSTPWEWIQHLARKHAEFVSGKEIEFFDKYFLRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HI61</accession>
    <name>A0A9Q1HI61_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ubiquitin thioesterase OTU</fullName>
            <ecNumber evidence="9">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_06598</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Hydrolase that can remove conjugated ubiquitin from proteins and may therefore play an important regulatory role at the level of protein turnover by preventing degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="9"/>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HI61"/>
    <dbReference type="OrthoDB" id="65596at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030968">
        <property type="term" value="P:endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22745">
        <property type="entry name" value="OTU_OTU1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17059">
        <property type="entry name" value="Ubl_OTU1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000096">
        <property type="entry name" value="Ubiquitin thioesterase OTU1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048857">
        <property type="entry name" value="OTU1_Ubl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003323">
        <property type="entry name" value="OTU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057766">
        <property type="entry name" value="Znf-C2H2_OTU1-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13312">
        <property type="entry name" value="HIV-INDUCED PROTEIN-7-LIKE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13312:SF0">
        <property type="entry name" value="UBIQUITIN THIOESTERASE OTU1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02338">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21403">
        <property type="entry name" value="OTU1_UBXL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24560">
        <property type="entry name" value="zf-C2H2_OTU1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50802">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5 9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="OTU" evidence="10">
        <location>
            <begin position="110"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU367104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8047564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="313" mass="34548" checksum="376D4BB6F3C8C52B" modified="2023-09-13" version="1">MTTAEHPLLLRIKSKEGNAKVDGLTSHSTLGHLLDKIQELTKIPSSSLKLLHGFPPCHVDTSNPKSKLSDFSLRTGDTIIVEESKIAGEALSKAEVSTRGGVNPGGRGILTRRVVPADNSCLFRSIALLMEGGETDGSRITELRNLIASVVCSRPAEYTDAVLGKSNTEYCLWIRNSETWGGAIEISILAEFYEVEINVVDTQSLRVDKFGENRGFKHCIYLIYDNIHYDPLVLESADENSSVETVFPVGDDWIFAQAMELAQEAKRSRQYTDLTGFTLRCLVCQLGLRGQRQAQQHAMATGHKNFAEYSNAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HK89</accession>
    <name>A0A9Q1HK89_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">MAM and LDL-receptor class A domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_00311</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HK89"/>
    <dbReference type="OrthoDB" id="6076617at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000998">
        <property type="entry name" value="MAM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051560">
        <property type="entry name" value="MAM_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23282">
        <property type="entry name" value="APICAL ENDOSOMAL GLYCOPROTEIN PRECURSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23282:SF101">
        <property type="entry name" value="MAM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00629">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50060">
        <property type="entry name" value="MAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MAM" evidence="4">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="13"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="144"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8048133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="161" mass="17849" checksum="EFFF7724F72B882F" modified="2023-09-13" version="1">MCDFETDFCDWQNPNDDEFDWQRDKGGTSSDNTVPGGDHPTNQAAFVTFSSGTGNFESFPLVSVLIDASTQACSTHNLQRTYVCSILALLVARHMPISCIICRRFMNNIVDFAAGSTRPPAPPCNSKQWLCPVEYTCIPKSKRCNDVEDCMDGSDEIDCDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HKT3</accession>
    <name>A0A9Q1HKT3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_01357</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HKT3"/>
    <dbReference type="OrthoDB" id="10063366at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF13">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8048871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="206" mass="24771" checksum="805FFBC68D36BB70" modified="2023-09-13" version="1">MQKEIEEREMQKEIEEREMQMQKETEEREMQMQMRREKEAREHEFRLKQLKLGVIKASDPKIGLDTGGFNVSKHVKFVPKFQEDNVEKFFNHFEKLGEQLKWPRDKWSILIQSNFTCKAQEVYSALSIEDSMDYDKVKKAILQAYELVPEAYRQKFRKYRKADTQSYVEFAYQKERHFDRWCASKKVSTFDALRTDAGRGIQTVCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YB32</accession>
    <name>A0A9Q0YB32_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_43883</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000514">
        <property type="protein sequence ID" value="KAJ8018236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YB32"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8018236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="131" mass="14980" checksum="C6A72D93D843387A" modified="2023-09-13" version="1">MCHQHQPYAYKQTNQTPAQMTEQDSCLGWFLEYLVVRGGNTTAARKSTQKHQRQVELLLQRLHLTGGVMRQLMAPSIPMRVVDLLQKEVMMPKTISSYSCSLKKLCRFLQFHRDVASCLRIPPTTLNTSAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YB95</accession>
    <name>A0A9Q0YB95_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_42858</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000150">
        <property type="protein sequence ID" value="KAJ8018895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YB95"/>
    <dbReference type="OrthoDB" id="10058988at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004748">
        <property type="term" value="F:ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009263">
        <property type="term" value="P:deoxyribonucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000358">
        <property type="entry name" value="RNR_small_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23409:SF21">
        <property type="entry name" value="CAPSID PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23409">
        <property type="entry name" value="RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE SMALL CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8018895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="255" mass="28147" checksum="31382304E7104693" modified="2023-09-13" version="1">MALVHDHSCECAKSELDLFTIPPTQTSIERGDWKEYRPLSSINTGGPIEFYVSGSGEEYIDLDQTQLYVRAKITKKDGSALEDDDAVGPVNLFLHSLFNQVDVALNGREISSATPTYPYRAMIETLLNYSHATKVDQLTSALFYKDTAGKMDVADPGAAAAAANMGLKKRSRFTNGSKEVELIGRLHSDIFCQEKYLLSGVDLRLKLTPSKRFFCPHVIGARSRIQSHVTASLFVRAESQNRAICAHCPRQGTGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YBH3</accession>
    <name>A0A9Q0YBH3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Chromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_42623</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000101">
        <property type="protein sequence ID" value="KAJ8019045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YBH3"/>
    <dbReference type="OrthoDB" id="124855at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8019045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="175" mass="20332" checksum="AB410EA8124CD2E3" modified="2023-09-13" version="1">MELRRMKRVDYKSVHRGASLRDKSYGKETTTWSTKRYFKLVVKDSRVVDGKQKLLVHYQGWDKKYDEWRLDSDILNTPAEAISGDALSYSAFQLTSSIKEKLVPLRKQNSEVTISIPVQKDIFTTFIENSSFESESRHGNYIYRFSNATVGEAAFAPNWWFRICNSAHDLCSHSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YG13</accession>
    <name>A0A9Q0YG13_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_39326</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YG13"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8021973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="240" mass="27181" checksum="93AD86772CA8F9FC" modified="2023-09-13" version="1">MDLDRQPKSEFAMGIEQQSTFREKDNTKWYVFAITVFAVALLAGFFGVFYILLSAPTPTFINNVSSDVEEHEGDLQVITRGVSFDMNGEDHYETMSAEGQKLTVQNYDDDFTAIFDYSRKILMTKNLTSNVCFFNCLEGMPDMGINDMDNARPFFFQQVSPGYKDPASDPNVEAMVLVQSAVIPPGYVVLTNDVIVSSQCTEQPSYWLEPKDGTMIRRDGEIYITGVIFGVYIEIHIWWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YH63</accession>
    <name>A0A9Q0YH63_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">QRFP-like peptide receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_37448</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YH63"/>
    <dbReference type="OrthoDB" id="9979846at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004983">
        <property type="term" value="F:neuropeptide Y receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14993">
        <property type="entry name" value="7tmA_CCKR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000611">
        <property type="entry name" value="NPY_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45695">
        <property type="entry name" value="LEUCOKININ RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45695:SF15">
        <property type="entry name" value="OPSIN RH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01012">
        <property type="entry name" value="NRPEPTIDEYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="52"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="91"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="132"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="171"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="222"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="281"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="318"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="71"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8022528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="383" mass="43995" checksum="3C1C2E9CD0C3813D" modified="2023-09-13" version="1">MDDELKSSVSEFSYKSKSLDELLAILNFSDKQAFIEFYKYDEIDVVHRLSPTAIGCLSAIFCLIFIVGLTGNLFLIVLVICKRNMQTVINIFNVSLAFADLLITLFCIPFTLVEQVTEDWVLGRFMCKILNYITTVSVICSVLTMTAIAVERHSAICHPLRSKVIQTPRRAIICSIVIWCFTLMLNMPLFVLIDIAFFKHPFQDITYEFCEWKNGEQEKRVSLCYVILLYFVPLLFVCFLYIRIVRKLWIRNAVAPANIIQSRVDIMRNSNIYKKKRATKMLMLVVLLYAMCWLPYHVFILMRDFKSSGLEDTEKNRFLMALFQLLGLTNSCNNPVIYGFMNDKFKTNIAALVKGKPKLKPSSTGKKTPFQKATPVGFVVTSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YH66</accession>
    <name>A0A9Q0YH66_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_38585</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YH66"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8021401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="80" mass="8907" checksum="EDD8CD10C74C5BD2" modified="2023-09-13" version="1">MVDHCPQSSHLAPDSLDVCFPSLPTMWVVLSMEALVVLTPCTSYLPPVAPTWKTQNVRNTLLCLKPPPPIKWWSLPVLPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YHU3</accession>
    <name>A0A9Q0YHU3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Nose resistant to fluoxetine protein 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_39017</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YHU3"/>
    <dbReference type="OrthoDB" id="207378at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002656">
        <property type="entry name" value="Acyl_transf_3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052728">
        <property type="entry name" value="O2_lipid_transport_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11161">
        <property type="entry name" value="O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11161:SF0">
        <property type="entry name" value="O-ACYLTRANSFERASE LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01757">
        <property type="entry name" value="Acyl_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040481480" evidence="3">
        <location>
            <begin position="23"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="221"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="368"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="408"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="450"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="516"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="545"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="595"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="630"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="663"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="698"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="737"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="domain" description="Acyltransferase 3" evidence="4">
        <location>
            <begin position="361"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="287"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="288"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8021731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="778" mass="88384" checksum="C52A4F61641EB14B" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YI65</accession>
    <name>A0A9Q0YI65_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_38063</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8023008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YI65"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8023008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="78" mass="8741" checksum="1A8B3C5309962158" modified="2023-09-13" version="1">MTLLTVYLGNLQTWDNRSTSRGYCMARSSPVDISAMVFCRPQVLAGSPNVALPVCCRSGRQAKVRTMRKDTFNRLMLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YK70</accession>
    <name>A0A9Q0YK70_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transcription factor HES-4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_39427</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8022049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YK70"/>
    <dbReference type="OrthoDB" id="6085656at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11410">
        <property type="entry name" value="bHLH_O_HES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.980">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050370">
        <property type="entry name" value="HES_HEY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003650">
        <property type="entry name" value="Orange_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10985">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, HES-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07527">
        <property type="entry name" value="Hairy_orange"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158457">
        <property type="entry name" value="Orange domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51054">
        <property type="entry name" value="ORANGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="6">
        <location>
            <begin position="7"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="Orange" evidence="7">
        <location>
            <begin position="85"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="214"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="214"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="227"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="239"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="275"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8022049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="462" mass="49394" checksum="9588028EB3FD19D0" modified="2023-09-13" version="1">MPLDKPRPKTPKRVMEKKRRARINDSLTELKNLVLQALNRNNPRHSKLEKADILEMTVKYLRGVHGRHVSGKASMTSDPGVSSQYRSGFAECMMEVKKFLDNSEAGSTLRLHLLDHLSRSCGVSHSLPSSAAGNGTCLSRSNCLSSSDVNMTHTLPVFRLPTTLTQATSHSAPSSSLAVDSVQSVQTMQINSPPTLPVMNIQLPTLPKVPAPTLSLPTPSIPLQTSPFPPPPPPLPRVQAKEKTPVREVTQDDKTSDVKTDDAAKASKLQGDTGGSSEATQPPSNAMPLVMGRTQGGDVAVVLPQSALPGGKFPTHLIPVYQPNLPNASNVVGAVTNTSPTGPFNAQSLPETTTASCTIPISSVASSPRAGTVAPVTWYTRPALLTTNIEQGQPVFAQQGTPVTITLPIQARIVQNIIPIQAAAISPITPGSSGNSLNHIPIRMANPNLQNIEENEFMWRPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YLJ0</accession>
    <name>A0A9Q0YLJ0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tetraspanin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_36146</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the tetraspanin (TM4SF) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YLJ0"/>
    <dbReference type="OrthoDB" id="10033535at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1450.10">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000301">
        <property type="entry name" value="Tetraspanin_animals"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018503">
        <property type="entry name" value="Tetraspanin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF519">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002419">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00259">
        <property type="entry name" value="TMFOUR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00421">
        <property type="entry name" value="TM4_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="51"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="86"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="215"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="147"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR002419-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8023651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="251" mass="27227" checksum="F5C9986045CD9589" modified="2023-09-13" version="1">MVAGCAPKIAKLLLILFNIAVWAVGCVLLSIGSWITYYATLFQDLFISDDLTVVSSLVIAAGVLLFIFSFMGCCGAWKEGTFLLKLYFVLLVVFLCIQATAGIIAFTFVSEIYDSMLEGMTTTINMTYGYYDSATYAVDTIQKEFNCCGALGPSDYRGCQGLQNNQSVPGSCCKTEGNGCQFGENASPGKPDPTKVDLINYNGCVEESLDYVEHYFYVLGGICIGVLVIEVTALVMICIFIRGIKKGKEVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YM16</accession>
    <name>A0A9Q0YM16_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">SH3 domain-binding protein 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_34999</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SH3BP5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YM16"/>
    <dbReference type="OrthoDB" id="446789at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004860">
        <property type="term" value="F:protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007940">
        <property type="entry name" value="SH3BP5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19423">
        <property type="entry name" value="SH3 DOMAIN-BINDING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19423:SF1">
        <property type="entry name" value="SH3 DOMAIN-BINDING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05276">
        <property type="entry name" value="SH3BP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="259"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="28"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="137"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="295"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="319"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="334"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="384"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="407"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8024937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="453" mass="51297" checksum="E0B030EDF08B67F2" modified="2023-09-13" version="1">MSANCTMTRERRSRTLSLIEDDGLDPEIKIGLERLNTTSEEINRLEKQLEDARQTYRQTLTELSSQVTEKAKQNKRSIRKAEAYHAASKVANEAHQQAQEAALKYHRAVDIFNAAKETISCAESAAFDEGGQRREFDEALQELLNHATMKLNEAEEEKTKSKIEHDQKAQVYVEAKQRVEFLARKFKSSIAKSRSFYEAKANFHVRVKENKECVEEILKKMSNARSEYSEALKFLENISDSVHEKREFKKLLDEQRRGSGVGAEATAEFDNLEISPDLPDIFGDDESNSEAGSMDSDRMLTDLQKRPLPSHPNPSMSRRVSGCSESETSSARSSMREKIDVETRLPHLRERGDGLSPVSKEDEDDCPFTAGETHLREEESEDSISEKNIDKKGDVEQSEESGFADASESEVSEIDIDDLSDDLETESPSPLKQEPVVRKTLKKLIPDEGEIWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YPK7</accession>
    <name>A0A9Q0YPK7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DNA cross-link repair 1A protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_32899</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YPK7"/>
    <dbReference type="OrthoDB" id="262529at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035312">
        <property type="term" value="F:5'-3' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16273">
        <property type="entry name" value="SNM1A-1C-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23240:SF6">
        <property type="entry name" value="DNA CROSS-LINK REPAIR 1A PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23240">
        <property type="entry name" value="DNA CROSS-LINK REPAIR PROTEIN PSO2/SNM1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="172"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="208"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="421"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="514"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="548"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="598"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="222"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="259"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="443"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="525"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="548"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="602"/>
            <end position="634"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8025361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="756" mass="83999" checksum="15218CCE030B2C81" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YPU9</accession>
    <name>A0A9Q0YPU9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_34111</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8026305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YPU9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009079">
        <property type="entry name" value="4_helix_cytokine-like_core"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47266">
        <property type="entry name" value="4-helical cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040512545" evidence="1">
        <location>
            <begin position="24"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8026305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="210" mass="24815" checksum="FF66F56D807B97B8" modified="2023-09-13" version="1">MADSKLITCVFVFLATLVMFIESLPIENERNRRDAYTLYSELVDKTERLRTMETTLLNNFKTNRLRNLAELTGVTVPNLSDLMTFAFRSTASPSQLLSEYRDHFMRYQIIMDFVLEDERNTTATPNFISDITNVSLLLSFTITRLENVMRHLNVTVTPEKIPEEESPYFTPRWERNIYQRRLRDCFVLEQLNQHLINAQNDFTKLRNDEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BAU2</accession>
    <name>A0A9Q1BAU2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF5641 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_40867</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8021096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BAU2"/>
    <dbReference type="OrthoDB" id="10050666at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040676">
        <property type="entry name" value="DUF5641"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331:SF1">
        <property type="entry name" value="GAG-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18701">
        <property type="entry name" value="DUF5641"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF5641" evidence="1">
        <location>
            <begin position="69"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8021096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="166" mass="19442" checksum="E0D8C8D2038EFFC9" modified="2023-09-13" version="1">MLGEQVVTDEVLHTVMAEVESILNSRPLTKLSPDPRDEEPLTPNHLLLLRVNKALPPGVFGITDSYGRRRWKQSQYMADQFWHRWLKEYLPLLQLRQKWTQPHRNFKVGDLVLVVNENGPRCQWPLRRVLEVYPDKEGLVRQVEVKVGDKIFKRPIVKLCLLESAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BDC6</accession>
    <name>A0A9Q1BDC6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">IQ domain-containing protein C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_40808</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8021049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BDC6"/>
    <dbReference type="OrthoDB" id="6161953at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="242"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="500"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="69"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="124"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="140"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="200"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="242"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="267"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="287"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="318"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="365"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="444"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8021049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="560" mass="62316" checksum="BF419FCCC79A90F8" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BDL9</accession>
    <name>A0A9Q1BDL9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transcription factor HES-4-A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_38784</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BDL9"/>
    <dbReference type="OrthoDB" id="6085656at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.980">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050370">
        <property type="entry name" value="HES_HEY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003650">
        <property type="entry name" value="Orange_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10985">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, HES-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07527">
        <property type="entry name" value="Hairy_orange"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00511">
        <property type="entry name" value="ORANGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158457">
        <property type="entry name" value="Orange domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51054">
        <property type="entry name" value="ORANGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Orange" evidence="6">
        <location>
            <begin position="32"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="74"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8021543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="206" mass="22462" checksum="EA26F18A2B39AC67" modified="2023-09-13" version="1">MEKADILDMTLRYVKDIQQKTREGKEPNIDQYHAGYSECLSQVSQFLTSCDSMCLETRRRLLTHLADKGLTVECSKPSQENPLPPAASPVDVKVSPETQAPSAQPLPPPTGIVYLLPAQSLQPVVQSTVVPQSQLSPTHTVFSTPTVSLLPTPPHSPIAVQQQSPTAENCYQTVIGGAHQVHQSTFSMVPVAIPVSSAKSEVWRPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BFQ2</accession>
    <name>A0A9Q1BFQ2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Solute carrier family 35 member G1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_35934</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BFQ2"/>
    <dbReference type="OrthoDB" id="306876at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22911">
        <property type="entry name" value="ACYL-MALONYL CONDENSING ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22911:SF137">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER G2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="42"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="75"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="104"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="131"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="163"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="195"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="224"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="256"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="282"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="3">
        <location>
            <begin position="13"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="3">
        <location>
            <begin position="165"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="306"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="306"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8023479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="327" mass="36458" checksum="51BA29291EE619E7" modified="2023-09-13" version="1">MYQICKSFCVSSVGILFAFLCAVSFSTEDILLYAIKDDFHPTMVLVFNSNIGFILCLIVLFIIRPKKTESFRELLLVIIYGTFNVCAHIGVIFAIMWIGPGSGIAIFFTTTVFTAIFSVICFKENPEVSDIFFIFCSTLGVAVLTRYMVLFEERLGKERISTVIAGVFVALTAAITYGVALIVGKTLSKFETPTILTILSYLLQYSVISLIICTSFNEWKVPETLISGMTVSATAITSFFGIYFEYLAVCYENPTRVTIILTSEVIMTFVGHFTLFSFSYHWTVLVGAGLIIASCIGLMIRKKTEDDQEVIEDEGDEKEESLPILDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BI96</accession>
    <name>A0A9Q1BI96_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">LRP2-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_34045</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">May act as an adapter that regulates LRP2 function.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8026252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BI96"/>
    <dbReference type="OrthoDB" id="2384430at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052323">
        <property type="entry name" value="LRP2-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006597">
        <property type="entry name" value="Sel1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44554">
        <property type="entry name" value="LRP2-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44554:SF1">
        <property type="entry name" value="LRP2-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08238">
        <property type="entry name" value="Sel1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00671">
        <property type="entry name" value="SEL1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0802">TPR repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8026252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="352" mass="39846" checksum="E39A66930719FBB9" modified="2023-09-13" version="1">MEHKLEKKQLSEEKLPADKSNSLLSVNSDIKSSVHILDDEDNKRREERELLEKVEKLLLGKVKEGEKTAIFQLGQFYFESERYEEAKYQFESIKDNDFQAQFQLGIIFYDGLGTKADPKKGFQYMLNIANSTDKRASHLVPFAQYNVGRAYFEGIGVKQSDVEAEKWWLLAADDGNPKGSVKAQTALGMYYSREDSLDLKKAFFWHSEATGNGSLESQGALGVMYHTGQGVRRDADSAFECLKEAADRGNVYAMGHLVAHFYATKMYTKAAELALRLTLLENIDEIKEATDCLPAFISKGIAMGCFYYARCLHLGQGVKKDKELAQTYYSKSFQFDGFVTQRLQNMVTHGII</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BIU5</accession>
    <name>A0A9Q1BIU5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_32530</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BIU5"/>
    <dbReference type="OrthoDB" id="7444419at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005312">
        <property type="entry name" value="DUF1759"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331:SF1">
        <property type="entry name" value="GAG-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03564">
        <property type="entry name" value="DUF1759"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="124"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8027398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="352" mass="39602" checksum="608CD4CD9FA5CAD6" modified="2023-09-13" version="1">MEKLKIKRRNRKGNLTRGIKELECLLANGATVENIRKVIAKAQLRFESIEQCHDELVELIEKDEDYEREQAWITECQQEYMTEMISSKKTEVNRIEENNNESGEEGDAKSNSGEAEVNVVEDTNDRKATSENGNNGACGFSGNASSSDNKVNDDLLGFFKTLSLPKPQIKQFDGDPFHYHAFMSTYMNMVANVVDDNVKLNTLMSLCVGKALESIKYCVLKTPSEGCKAAIETLKYRFGNTAVFVQAWINKILSCPKVDPGKMTEYADDLNNCFEALSALGYLHELNNQSSLKQIVDKLPKFLQNRWKRENVRLKMTGVIPGLENIVKFVKASAMEINDPVFGNHENSSGKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BIY1</accession>
    <name>A0A9Q1BIY1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_32623</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BIY1"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8027472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="92" mass="10751" checksum="A179EE78D5107D6A" modified="2023-09-13" version="1">MVVLGIEYEKYVKLGKEIGLSGQGLLDFANNREAIDKKERAKLEKEAREEKDKQRQHEKDMKDFELKLLEERQRSALNDNTDVNSSRTVEVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BL45</accession>
    <name>A0A9Q1BL45_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_30904</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BL45"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8028613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="65" mass="7590" checksum="843498FEEC7EF0FB" modified="2023-09-13" version="1">MPLKKPVLYLVICKQFSTSAVVLLRKLLSDDIDHKNNTEIRFEARGARENFESLRRICEERTALI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BM06</accession>
    <name>A0A9Q1BM06_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Protocadherin Fat 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_28477</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BM06"/>
    <dbReference type="OrthoDB" id="283575at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045197">
        <property type="term" value="P:establishment or maintenance of epithelial cell apical/basal polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007157">
        <property type="term" value="P:heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000185">
        <property type="entry name" value="basement membrane-specific heparan sulfate proteoglycan core protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000012">
        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000143">
        <property type="entry name" value="Protein crumbs 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013032">
        <property type="entry name" value="EGF-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051022">
        <property type="entry name" value="Notch_Cell-Fate_Det"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24049">
        <property type="entry name" value="CRUMBS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24049:SF22">
        <property type="entry name" value="DROSOPHILA CRUMBS HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12661">
        <property type="entry name" value="hEGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00010">
        <property type="entry name" value="EGFBLOOD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1 6" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040119889" evidence="7">
        <location>
            <begin position="20"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="8">
        <location>
            <begin position="13"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="8">
        <location>
            <begin position="51"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="8">
        <location>
            <begin position="89"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="8">
        <location>
            <begin position="127"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="39"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="77"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="115"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8029147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="175" mass="18765" checksum="3E8309BB1513BEF4" modified="2023-09-13" version="1">MKTKCIKLSFLLDMSSCSSEPCFNGGSCVEQLYGYTCVCQHGFHGDNCETEIPTCATEPCHNGGTCVTQAGGYVCVCRGGFTGKHCHNKTSSCSIEPCFNGGSCVEQLHGYTCACQLGFRGDNCEIELPSCATELCNNGGTCVTPDGGYVCVCRGEFNGQHWKIKAFVYSTSFTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BNY0</accession>
    <name>A0A9Q1BNY0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Protein KRTCAP2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_29442</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the KRTCAP2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BNY0"/>
    <dbReference type="OrthoDB" id="1111004at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018614">
        <property type="entry name" value="KRTCAP2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32001">
        <property type="entry name" value="KERATINOCYTE-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32001:SF1">
        <property type="entry name" value="KERATINOCYTE-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09775">
        <property type="entry name" value="Keratin_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="75"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8029914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="130" mass="14214" checksum="1656769253FB3B1C" modified="2023-09-13" version="1">MAVSSGVSFILSSLLVVVLFAAMQLFQKELASSQWLTILGGALGAKVFVFTLTAVNNLEIVVFGKGFQAKLFPEVFGSMLFAMFVSGLVHRVCVTTCLLFSILALYYLNKISQRIYAPPPTVQTTGKKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BPL6</accession>
    <name>A0A9Q1BPL6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_26817</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BPL6"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8030398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="90" mass="11025" checksum="6915612520643339" modified="2023-09-13" version="1">MDGWMDGWMDGWMDGWMDGWMDGWMDGWMDGWMDGWVDGWMGWMDGWMDGWMDGWMDGWMDGWMDGWMDGWMDGWMDGWMDGWMDGWIDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BPM0</accession>
    <name>A0A9Q1BPM0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Complement C1q tumor necrosis factor-related protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_26621</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BPM0"/>
    <dbReference type="OrthoDB" id="6138508at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001073">
        <property type="entry name" value="C1q_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050822">
        <property type="entry name" value="Cerebellin_Synaptic_Org"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008983">
        <property type="entry name" value="Tumour_necrosis_fac-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22923">
        <property type="entry name" value="CEREBELLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22923:SF62">
        <property type="entry name" value="CVP18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00386">
        <property type="entry name" value="C1q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00007">
        <property type="entry name" value="COMPLEMNTC1Q"/>
    </dbReference>
    <dbReference type="SMART" id="SM00110">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49842">
        <property type="entry name" value="TNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50871">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040205881" evidence="6">
        <location>
            <begin position="20"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="C1q" evidence="7">
        <location>
            <begin position="323"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="217"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="82"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="170"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="225"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="248"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8030259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="459" mass="51726" checksum="8C7113B1DEE0BCD0" modified="2023-09-13" version="1">MKSISACVFFATVLVIGGAVKAPKRKPNPTPVTDEALQRRIVSLEASQETVQRKVTQMKITGSNIEQRLTALEEGIGTQEELTTVKELLQQSEAKNQELMKMLSNYERFFGALYAETPEAFDPTEAVDVRMTKLNSYLGNREAELLSLRPAPPTIVSNATVNEDLLGTALYELEEQKSQLDRKVRNLEDRIDRITGQIPDHANARINRVETELALQNEEEGTVRVNCQQSNSGSAGPPGQLKNKTKGNAREDCRTKRSIDGSTNQLSDDEVQDRLERLENLVLLLLQLPTINQTLGQSIDRSLSSSQLEELTDEIIALRGSKPDSKKIAFSVARTTPLLGESYEQILEFDYVFVNRGGHFKEENSTFICEVTGYYFVSFTIRSFDGHVLGINLMKNDDHVTSVFTDAHHRNVMESQSVIVHLKKGDHLWLRHPPSERFGIHSDRYRYTTFSAFMIYKGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BPY7</accession>
    <name>A0A9Q1BPY7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">X-ray radiation resistance-associated protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_26872</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BPY7"/>
    <dbReference type="OrthoDB" id="1687175at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025875">
        <property type="entry name" value="Leu-rich_rpt_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22710">
        <property type="entry name" value="X-RAY RADIATION RESISTANCE ASSOCIATED PROTEIN 1 XRRA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22710:SF2">
        <property type="entry name" value="X-RAY RADIATION RESISTANCE-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12799">
        <property type="entry name" value="LRR_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="152"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="321"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="612"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="541"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="162"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="450"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="621"/>
            <end position="631"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8030439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="631" mass="70679" checksum="CCBF8BF9F31B3748" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BQJ3</accession>
    <name>A0A9Q1BQJ3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">B(0,+)-type amino acid transporter 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_27292</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BQJ3"/>
    <dbReference type="OrthoDB" id="10062876at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050598">
        <property type="entry name" value="AminoAcid_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785:SF375">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="49"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="108"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="163"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8030786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="219" mass="23441" checksum="DF852844BE9497EA" modified="2023-09-13" version="1">MDHEKVALSRSLGLFHCIGVLAGCIIGTAIFISPAAILSGVNGSVGLSFVIWIASALLGTSEALCFAELGSLFTKSGGEFIYLMESFGPLVAFLRVWSNVVVQGPANAALQTMVIATYVVAPFLGDCHELPWLAVRLLAACAMWLIFFVNCVSVPLAVRIQSFMTVAKITGLVVLILTGMVFLCQGYTDSFREPFQMYSFKLGGLSSAFLAGRYAFAGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BRX1</accession>
    <name>A0A9Q1BRX1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Staphylococcal nuclease domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_24784</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BRX1"/>
    <dbReference type="OrthoDB" id="10023235at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031332">
        <property type="term" value="C:RNAi effector complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006402">
        <property type="term" value="P:mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00175">
        <property type="entry name" value="SNc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd20433">
        <property type="entry name" value="Tudor_TDRD11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000018">
        <property type="entry name" value="Serine/threonine-protein kinase 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.90:FF:000001">
        <property type="entry name" value="Staphylococcal nuclease domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.90:FF:000002">
        <property type="entry name" value="Staphylococcal nuclease domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.90:FF:000003">
        <property type="entry name" value="Staphylococcal nuclease domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.90:FF:000004">
        <property type="entry name" value="Staphylococcal nuclease domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.90:FF:000005">
        <property type="entry name" value="Staphylococcal nuclease domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.90">
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016685">
        <property type="entry name" value="Silence_cplx_Nase-comp_TudorSN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035437">
        <property type="entry name" value="SNase_OB-fold_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016071">
        <property type="entry name" value="Staphylococal_nuclease_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002999">
        <property type="entry name" value="Tudor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047386">
        <property type="entry name" value="Tudor_TDRD11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12302">
        <property type="entry name" value="EBNA2 BINDING PROTEIN P100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12302:SF2">
        <property type="entry name" value="STAPHYLOCOCCAL NUCLEASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00565">
        <property type="entry name" value="SNase"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00567">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017179">
        <property type="entry name" value="RISC-Tudor-SN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00318">
        <property type="entry name" value="SNc"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00333">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50199">
        <property type="entry name" value="Staphylococcal nuclease"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50830">
        <property type="entry name" value="TNASE_3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50304">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="TNase-like" evidence="8">
        <location>
            <begin position="10"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="TNase-like" evidence="8">
        <location>
            <begin position="187"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="TNase-like" evidence="8">
        <location>
            <begin position="333"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="domain" description="TNase-like" evidence="8">
        <location>
            <begin position="513"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="domain" description="Tudor" evidence="7">
        <location>
            <begin position="714"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="475"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="479"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR017179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8031554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="892" mass="99920" checksum="5ED8CB7C3BF934E0" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BTS2</accession>
    <name>A0A9Q1BTS2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Integrase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_23155</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BTS2"/>
    <dbReference type="OrthoDB" id="6110546at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon gypsy-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF15">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase zinc-binding" evidence="1">
        <location>
            <begin position="70"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF17921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8033033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="166" mass="19183" checksum="ED3BA5AD46D023F6" modified="2023-09-13" version="1">MSDTLHRDQLSVQQQHDEELQPLIAGAFTEDESKDVPVCFYFKNNILMLKWRPPDAPLDEEWRIYHQIVVPRTYRREILSIAHEMPFAGHLGVNKTYHRILNHFYWPKLKSDVAKFGKTCQSCQIIGEPNQNIPVAPLKPIPAFEEPFSRVIIDCVGPLPKSRAGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BUW1</accession>
    <name>A0A9Q1BUW1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_23455</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BUW1"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8033272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="52" mass="6237" checksum="C464056B195536F4" modified="2023-09-13" version="1">MKLFLTHPYQCFYNTAMSEINILFVLNYKKTTTHSFSKIPGLRFRYLKRMGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BWN4</accession>
    <name>A0A9Q1BWN4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">4a-hydroxytetrahydrobiopterin dehydratase</fullName>
            <ecNumber evidence="3">4.2.1.96</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">4-alpha-hydroxy-tetrahydropterin dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_21625</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(4aS,6R)-4a-hydroxy-L-erythro-5,6,7,8-tetrahydrobiopterin = (6R)-L-erythro-6,7-dihydrobiopterin + H2O</text>
            <dbReference type="Rhea" id="RHEA:11920"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15642"/>
            <dbReference type="ChEBI" id="CHEBI:43120"/>
            <dbReference type="EC" id="4.2.1.96"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the pterin-4-alpha-carbinolamine dehydratase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.96" evidence="3"/>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BWN4"/>
    <dbReference type="OrthoDB" id="277398at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008124">
        <property type="term" value="F:4-alpha-hydroxytetrahydrobiopterin dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006729">
        <property type="term" value="P:tetrahydrobiopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.20">
        <property type="entry name" value="Transcriptional coactivator/pterin dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036428">
        <property type="entry name" value="PCD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001533">
        <property type="entry name" value="Pterin_deHydtase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12599">
        <property type="entry name" value="PTERIN-4-ALPHA-CARBINOLAMINE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12599:SF0">
        <property type="entry name" value="PTERIN-4-ALPHA-CARBINOLAMINE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01329">
        <property type="entry name" value="Pterin_4a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55248">
        <property type="entry name" value="PCD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00030497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8034683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="52" mass="5699" checksum="EB83D0CE668D0DE0" modified="2023-09-13" version="1">MTRCALKAESINHHPKWSNVYNRVAVTLTTHDVGGLSNLDLNMAVFMAELAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BWR3</accession>
    <name>A0A9Q1BWR3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ankyrin repeat domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_20866</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BWR3"/>
    <dbReference type="OrthoDB" id="539213at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8034147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="72" mass="8373" checksum="A5A737496410486C" modified="2023-09-13" version="1">MDDFKYNDSLWGAAMNGNIDRVREILSDPAGIAQINQTNKYGYTPLQLQYAAFFYYKNKRFLIIKLFNASPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-06-18" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BXZ2</accession>
    <name>A0A9Q1BXZ2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">DNA polymerase epsilon catalytic subunit</fullName>
            <ecNumber evidence="15">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HOLleu_22704</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Nanhai2018</strain>
            <tissue evidence="18">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">DNA polymerase II participates in chromosomal DNA replication.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20 21">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="15"/>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10060449at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008622">
        <property type="term" value="C:epsilon DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008310">
        <property type="term" value="F:single-stranded DNA 3'-5' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006287">
        <property type="term" value="P:base-excision repair, gap-filling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045004">
        <property type="term" value="P:DNA replication proofreading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006272">
        <property type="term" value="P:leading strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006297">
        <property type="term" value="P:nucleotide-excision repair, DNA gap filling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05779">
        <property type="entry name" value="DNA_polB_epsilon_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05535">
        <property type="entry name" value="POLBc_epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.60:FF:000002">
        <property type="entry name" value="DNA polymerase epsilon catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.342.10:FF:000023">
        <property type="entry name" value="DNA polymerase epsilon catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000010">
        <property type="entry name" value="DNA polymerase epsilon catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1600.10:FF:000006">
        <property type="entry name" value="DNA polymerase epsilon catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.60">
        <property type="entry name" value="DNA polymerase family B, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.342.10">
        <property type="entry name" value="DNA Polymerase, chain B, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1600.10">
        <property type="entry name" value="Palm domain of DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006172">
        <property type="entry name" value="DNA-dir_DNA_pol_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006133">
        <property type="entry name" value="DNA-dir_DNA_pol_B_exonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042087">
        <property type="entry name" value="DNA_pol_B_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013697">
        <property type="entry name" value="DNA_pol_e_suA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023211">
        <property type="entry name" value="DNA_pol_palm_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029703">
        <property type="entry name" value="POL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055191">
        <property type="entry name" value="POL2_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054475">
        <property type="entry name" value="Znf-DPOE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10670">
        <property type="entry name" value="DNA POLYMERASE EPSILON CATALYTIC SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10670:SF0">
        <property type="entry name" value="DNA POLYMERASE EPSILON CATALYTIC SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03104">
        <property type="entry name" value="DNA_pol_B_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08490">
        <property type="entry name" value="DUF1744"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22634">
        <property type="entry name" value="POL2_thumb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22912">
        <property type="entry name" value="zf-DPOE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23250">
        <property type="entry name" value="zf_DPOE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01159">
        <property type="entry name" value="DUF1744"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00486">
        <property type="entry name" value="POLBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 15" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6 15" id="KW-0235">DNA replication</keyword>
    <keyword evidence="13 15" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10 15" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="11 15" id="KW-0408">Iron</keyword>
    <keyword evidence="12 15" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 15" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="14 15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 15" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DNA polymerase epsilon catalytic subunit A C-terminal" evidence="17">
        <location>
            <begin position="1548"/>
            <end position="1950"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1230"/>
            <end position="1285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1309"/>
            <end position="1329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1953"/>
            <end position="1978"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="2026"/>
            <end position="2055"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="2041"/>
            <end position="2055"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU365029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM01159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ8035463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="2312" mass="264348" checksum="18EFBF32FF709729" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C058</accession>
    <name>A0A9Q1C058_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Mitochondrial coenzyme A transporter SLC25A42</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_19979</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C058"/>
    <dbReference type="OrthoDB" id="270584at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24089">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="7">
        <location>
            <begin position="46"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="7">
        <location>
            <begin position="141"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="7">
        <location>
            <begin position="236"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8036107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="336" mass="37425" checksum="139DE2129534FECD" modified="2023-09-13" version="1">MVDFEHEEAATELIQEDSHPMVNIAIQVKRPEAVLPQQKVEETTWQTIIINLISGAVAGAVAKTVIAPLDRTKILFQTSDMRFGVRQVAGVLNNVYRNEGVFALWRGNSATMVRIIPYAALQYSAHEQYKKLLRPSNSQNLAPFPRFCAGSMAGVTASALTYPLDVLRARMAVTERTRYSGMLSMCLHTLKHQGPRAFYQGFLPTMLGAIPYGGTSFFTYETFKKRHRELTNNREPLPVERLCYGAVAGLCGQSSSYPLDVIRRRMQTAGITKYSYKTIWKTAMEIYREGGIKGGLYKGLSMNWIKGPIAVGISFTVFDLSKNALSNTEVFSSGVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C267</accession>
    <name>A0A9Q1C267_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">WD repeat domain phosphoinositide-interacting protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_17720</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the WD repeat PROPPIN family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C267"/>
    <dbReference type="OrthoDB" id="1667587at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034497">
        <property type="term" value="P:protein localization to phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000145">
        <property type="entry name" value="WD repeat domain phosphoinositide-interacting protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048720">
        <property type="entry name" value="PROPPIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11227">
        <property type="entry name" value="WD-REPEAT PROTEIN INTERACTING WITH PHOSPHOINOSIDES WIPI -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21032">
        <property type="entry name" value="PROPPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0072">Autophagy</keyword>
    <keyword evidence="5" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="378"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="389"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8037012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="485" mass="52871" checksum="63341794D77612FA" modified="2023-09-13" version="1">MIRSKLTRKYEAYGAVTAREMNLTSQHADPNSNLLFGNFNQDFTSLAVGTKIGYRLFSLSSVDKLEQIYENAETSDICIVERLFSSSLVAIVSLSAPRKLKVCHFKKGTEICNYSYSNTILAVRLNRKRLIVALEESLYIHNIRDMKVLHTIRDTPANPEGLCALSVNGDNCYLAYPGSNQVGEVQIFDTENLHAVTMIPAHDSPLAALAFDPSGTKIATASEKGTVIRVFTIPEGTKLFEFRRGVKRCVSIGCLTFSADSLFLCASSNTETVHIFKLEHPKDSARPTEEPSGWMGYLGKALMTPASYLPTQVTDVFTQGRAFAQVKLPFSQLKNVCAIAMLPKPRVLIAAADGYLYMYNLDPSDGGDCTLLKQHRLDGQQDPNIPVVSSEASGESAGAEGGSEAPIQPTFADVAQKTPPRTSPIRGGGGKETTGHTRASSDPSAEDLLSQEADYQPDTHATGLNTLRLDDDNEFPPMIHHTDPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C551</accession>
    <name>A0A9Q1C551_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GH3 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_15727</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C551"/>
    <dbReference type="OrthoDB" id="10004661at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016881">
        <property type="term" value="F:acid-amino acid ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004993">
        <property type="entry name" value="GH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056985">
        <property type="entry name" value="GH3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31901">
        <property type="entry name" value="GH3 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31901:SF9">
        <property type="entry name" value="GH3 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25146">
        <property type="entry name" value="GH3_N_vert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GH3" evidence="1">
        <location>
            <begin position="33"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF25146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8038334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="130" mass="14755" checksum="8BDBB7A9AF2A49D9" modified="2023-09-13" version="1">MIVDDLSEEKLNPDLKLSPDIGKYLIRALKSGDPIRAAEVRRELEKGSDGIMKRLWPNLTVISTIDNVGLRQRIKKSLAKGVEMYSPFYISPDNLLGLNLLPYNKGGVEYTLHLSENVFEFIQEKDVSYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C5P9</accession>
    <name>A0A9Q1C5P9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Proteasome subunit beta type-1-B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_16793</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C5P9"/>
    <dbReference type="OrthoDB" id="268479at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005839">
        <property type="term" value="C:proteasome core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF2">
        <property type="entry name" value="PROTEASOME SUBUNIT BETA TYPE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 4" id="KW-0647">Proteasome</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8039161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="140" mass="15754" checksum="A70487A042701E70" modified="2023-09-13" version="1">MYMHDHNKEMSCNAVAAMLSKVLYYRRFFPYYTYNIIAGLDEEGKGCVYSFDPVGSYEREVYRAAGSASSLLQPLLDNQVGGKNQEGFVTAPVSLEKAIALIKDVFISAAERDIYTGDGIVIHIITKDGIKEERFPLRRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C644</accession>
    <name>A0A9Q1C644_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_16665</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C644"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8039067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="81" mass="9535" checksum="84CC2F14DF851487" modified="2023-09-13" version="1">MIIFLPQQGNDKNGLYLDRLSHHKYSENRLEASFNNQGGSSHDWQGGTEKYKIMCMLLSFTCWKTTTKHREKAKLWVKVMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C7I5</accession>
    <name>A0A9Q1C7I5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_13722</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8039653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C7I5"/>
    <dbReference type="OrthoDB" id="10000497at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon gypsy-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF13">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="2">
        <location>
            <begin position="654"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="203"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8039653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="739" mass="83791" checksum="042973E62FB9A13F" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C7N1</accession>
    <name>A0A9Q1C7N1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_17299</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C7N1"/>
    <dbReference type="OrthoDB" id="5988448at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09275">
        <property type="entry name" value="RNase_HI_RT_DIRS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03714">
        <property type="entry name" value="RT_DIRS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="entry name" value="Intergrase catalytic core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044068">
        <property type="entry name" value="CB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052055">
        <property type="entry name" value="Hepadnavirus_pol/RT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010998">
        <property type="entry name" value="Integrase_recombinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041373">
        <property type="entry name" value="RT_RNaseH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33050">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33050:SF7">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17917">
        <property type="entry name" value="RT_RNaseH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51900">
        <property type="entry name" value="CB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="9">
        <location>
            <begin position="1"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="Core-binding (CB)" evidence="11">
        <location>
            <begin position="491"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="domain" description="Tyr recombinase" evidence="10">
        <location>
            <begin position="594"/>
            <end position="798"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8039544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="802" mass="91293" checksum="DE305AD74E6DCE9F" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C8C7</accession>
    <name>A0A9Q1C8C7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Alpha-2,8-sialyltransferase 8F</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_14227</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 29 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C8C7"/>
    <dbReference type="OrthoDB" id="10264956at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003828">
        <property type="term" value="F:alpha-N-acetylneuraminate alpha-2,8-sialyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006491">
        <property type="term" value="P:N-glycan processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009311">
        <property type="term" value="P:oligosaccharide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23963">
        <property type="entry name" value="GT29_ST8SIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1480.20">
        <property type="entry name" value="Glycosyl transferase family 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001675">
        <property type="entry name" value="Glyco_trans_29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050943">
        <property type="entry name" value="Glycosyltr_29_Sialyltrsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038578">
        <property type="entry name" value="GT29-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012163">
        <property type="entry name" value="Sialyl_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11987">
        <property type="entry name" value="ALPHA-2,8-SIALYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11987:SF54">
        <property type="entry name" value="ST8 ALPHA-N-ACETYL-NEURAMINIDE ALPHA-2,8-SIALYLTRANSFERASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00777">
        <property type="entry name" value="Glyco_transf_29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005557">
        <property type="entry name" value="Sialyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="156"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR005557-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8040040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="369" mass="43026" checksum="8C12D59298950209" modified="2023-09-13" version="1">MGRWKSRRKYVCLYLPFLIFCFMGIGLLHRNEVVSRTRSASKLNLHSIMEEYDSTSNRTGQIWNFATSETETIYLYKTLLTKEHFFNASNLAIFRNELRLGLGSYFNNNVVIDQQNTPRNYTFTFESKKKSSKKQISDDIHKRLPNNISYEVIPRCSIVGNSGLILNSTCGNDIDKADFVFRCNAAPLKAFVVDAGSKSNFTTFNPSILETRYQKLSQPDNVTRFLNDMKEYRELLWAPCYTAVTMENCTKVLKLYNLTENKFVLGYPSHFKKVQEFWKTRGFKKRITTGFYLVNVAISRCRELHLYGFWPFLQEVDSAIKSVRYHYFEEIKPDLSKVEKVHDMQSEFSVLLQLHNLGIIKIHIGRCNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C9J4</accession>
    <name>A0A9Q1C9J4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">WD repeat and SOCS box-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_15052</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C9J4"/>
    <dbReference type="OrthoDB" id="2013972at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001496">
        <property type="entry name" value="SOCS_box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036036">
        <property type="entry name" value="SOCS_box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051983">
        <property type="entry name" value="WSB_SOCS-box_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15622:SF2">
        <property type="entry name" value="U4_U6 SMALL NUCLEAR RIBONUCLEOPROTEIN PRP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15622">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07525">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00253">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00969">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158235">
        <property type="entry name" value="SOCS box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50225">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="2">
        <location>
            <begin position="409"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="SOCS box" evidence="3">
        <location>
            <begin position="435"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8040688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="489" mass="54172" checksum="78D1489D074E6C60" modified="2023-09-13" version="1">MGNTNGVQFSRAKSNYSFPHYKWRPMKSPSNPTSSSWSGGNGDHEDNSHGQLAMVIDLARAYGVKRRDLQSVSLTTDCVFPPYDDSRLITCISIQGISPAARRVWIRSAVLLMEDVINIWYMNGQKKPSCGGPIIHDQKALLTIRKRNNWPVAASVRPSQDARFLCYPDSNHLEFRHVRNSSYVFVRDAIFKRSNYYKCICISPDDTRAVGIIAVNGDANLFVCSTDLFYLYWTRSCLKLFPRLAELGSLHNNTDMSCAFSPDGSLIAVTSSTGAMFLVSRYTLRLVTGVLPRRTPQSEHDVLLASAAAFDFHPLVASSLIACCTSDKMLALFSVSSERFTKLVLFEDCIGSPTVLKYNPTGTMLAMAGDNSNITIYNADTLDVHTIVDTTVPCPRCAAKIPLHNPGNLNPQQTTFLSANFSHDSMHIAASSTDGLVRVWKVPVVQTLLHLSRLTILKVTPIHKIYKLPLPDVLKSFIVCKPLETGSLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C9S0</accession>
    <name>A0A9Q1C9S0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_12737</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C9S0"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8041823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="65" mass="7956" checksum="7955898AB43AF065" modified="2023-09-13" version="1">MDQDNPMPKSDESTRSKKELIFESINPRIHHYRRCQRVRNVFLPTLVHWTNRKKEKCFPLMETNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C9Y8</accession>
    <name>A0A9Q1C9Y8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Cuticle-degrading serine protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_11812</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the peptidase S8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C9Y8"/>
    <dbReference type="OrthoDB" id="206201at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04077">
        <property type="entry name" value="Peptidases_S8_PCSK9_ProteinaseK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000014">
        <property type="entry name" value="Proteinase K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.80">
        <property type="entry name" value="Peptidase S8 propeptide/proteinase inhibitor I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034193">
        <property type="entry name" value="PCSK9_ProteinaseK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050131">
        <property type="entry name" value="Peptidase_S8_subtilisin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010259">
        <property type="entry name" value="S8pro/Inhibitor_I9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037045">
        <property type="entry name" value="S8pro/Inhibitor_I9_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806:SF58">
        <property type="entry name" value="ALKALINE PROTEASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806">
        <property type="entry name" value="PEPTIDASE S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05922">
        <property type="entry name" value="Inhibitor_I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 5 6" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0720">Serine protease</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="81"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="Inhibitor I9" evidence="9">
        <location>
            <begin position="128"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="8">
        <location>
            <begin position="204"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="213"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="248"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF05922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8041092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="454" mass="48028" checksum="CBE9A743500F1112" modified="2023-09-13" version="1">MLCDVKISIHARHEITNKNICSLVLSVGTRNYIMNKKRNKISTDNSTEQKWLNSGPFRIEIHWFLKCLQETQLLTQVKMKFLLVVLFVAAASALAPLHVKKDKIEGSYLVNFKENVDVDSALKTVRELPFFASVGGRIDREYRNVVKGFAATLSEKALELIRNLDIVDFVEEDQMMYALVEWGLDRIDQRSLPLNNGYSPKGTGSGVSVYVLDTGINANHNDFGGRASTSSSMDFHSNPNNGVDCHGHGTHCAGTVGGSSYGVAKSVTLYGVRVLGCFGSGSNSGIVNGMDYVGGRSGSRVASMSLGGGASITTDNAVTNLYNDGVPVIVAAGNENQDACNVSPARASRVAYTVGATDSSDAIASFSNWGTCVDIFAPGVSIRSASHSSNTGSTTMSGTSMACPHVAGAAAIERASTSSVSSIYSALTNNASSGKISGLTLSKSSSPNLLLYVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CAU8</accession>
    <name>A0A9Q1CAU8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">26S proteasome non-ATPase regulatory subunit 7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">26S proteasome regulatory subunit RPN8</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_11919</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M67A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CAU8"/>
    <dbReference type="OrthoDB" id="10256771at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005838">
        <property type="term" value="C:proteasome regulatory particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08062">
        <property type="entry name" value="MPN_RPN7_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000009">
        <property type="entry name" value="26S proteasome non-ATPase regulatory subunit 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024969">
        <property type="entry name" value="EIF3F/CSN6-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000555">
        <property type="entry name" value="JAMM/MPN+_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033858">
        <property type="entry name" value="MPN_RPN7_8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10540:SF7">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10540">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01398">
        <property type="entry name" value="JAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13012">
        <property type="entry name" value="MitMem_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00232">
        <property type="entry name" value="JAB_MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0647">Proteasome</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MPN" evidence="6">
        <location>
            <begin position="14"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="287"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00071765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00075094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8041179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="340" mass="38795" checksum="1BE444C8F77A2970" modified="2023-09-13" version="1">MTTPAIPTFEIKRVVVHPLVLLSVVDHFNRIKNQRRVVGVLLGSWRQGILDIANSFAVPFDEDDKDNSVWFLDHDYLENMYGMFKKVNAREKIVGWYHTGPKLHQNDIQVNELIRKYCPNSVLVIIDAKPKEIGLPTEAYIAVEEVHDDGTPTSKTFEHIPSEMGAEEAEEVGVEHLLRDIKDTSVGTLSQRITAQVLGLKGLHSQIENISSYLQKVNKGELPVNHTIIYQLQDVFNLLPDVNVSEFIRSLTVKTNDQMLVVYLASLIRSVVALHTLIDNKVQNRDLEKQEGKEKKEDKEKKDKDKNDKTNDKTKDDKDDKSADKKKDDKTKDTKKDSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CAZ4</accession>
    <name>A0A9Q1CAZ4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Bone morphogenetic protein 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_12469</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the TGF-beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CAZ4"/>
    <dbReference type="OrthoDB" id="6427922at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13756">
        <property type="entry name" value="TGF_beta_BMPs_GDFs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001839">
        <property type="entry name" value="TGF-b_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015615">
        <property type="entry name" value="TGF-beta-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848:SF263">
        <property type="entry name" value="PROTEIN DECAPENTAPLEGIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848">
        <property type="entry name" value="TGF-BETA FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00019">
        <property type="entry name" value="TGF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00204">
        <property type="entry name" value="TGFB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51362">
        <property type="entry name" value="TGF_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0339">Growth factor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040428781" evidence="7">
        <location>
            <begin position="34"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="TGF-beta family profile" evidence="8">
        <location>
            <begin position="256"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8041602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="377" mass="42970" checksum="BB70BF4277296CB2" modified="2023-09-13" version="1">MKLSLARLGLRAIGNIPKTLFFVSFMLHVLVLASDPQSTNSHVSRQSSFSAGPQEVLEPPQFLIDLYSGAIEDVGLKLEGSSRVVAVTLQKGVKRGNNKFFLSFKRPDIYRVRSVKLAMTKLRHSSIVTRYSARDNSSTKVNEPALRVFRKKKFAGKRDCFRPIFKSRTYDIFNVTEFYTNVLENSKPDVMLEFDIAFHCCCGESPSWVGDVYQLDSQQAHSPLLLFYDEVSIGILNNSVIQERMSGNNHFLRNARSIRSARREYQPPCKLRPWVVKVKDLGWSNYVYAPSSLKINYCRGECHFLGNAKATHHALITYLFLNNRAKSQRNQGIIRKMKWACCAPDQFQSINVIYQDTDGSIVFDEWDNVRAVSCGCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CB15</accession>
    <name>A0A9Q1CB15_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_12504</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CB15"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8041632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="103" mass="11528" checksum="7398D03886DB8734" modified="2023-09-13" version="1">MTYTNYSYVYIVCAVVALKIGLLIFWICRRANLAENRRRTIIVRQIHAPQQVPYENLGNSPASEWQGPYPQVAPAGVTVVTNPKMDQPPSYSESVSGVQGPKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CB50</accession>
    <name>A0A9Q1CB50_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_12996</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8042032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CB50"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="78"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8042032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="165" mass="19455" checksum="3380CFD53D0AFAFD" modified="2023-09-13" version="1">MPFNLDKFVASPSVEELDSLKKSEIVKVAKHYGIEFQPLMRKDEIKRYVLEYLVDESILPSTVLETAITVPTDNTFELKRLELEMNKEIRLKEMEREREREEKAREHEFRLKQLELGVIKGSDPKIGLDTGVFDVSKHVKFVPKFQEDNVEKFFNHFEKLGEQLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CB91</accession>
    <name>A0A9Q1CB91_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_13120</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8042131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CB91"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8042131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="124" mass="13720" checksum="ECF53276C959898D" modified="2023-09-13" version="1">MPHIERKNPIVFGGGQRSPGITGGQTLKTLLTQYLEMRYGMRKNPIIFGGGERSSGVTGGQTLKTSLTRYLKMPHIERKNPIVFGGGQRSPGVTGGQTLKTLLTQYLELQSLDEFHTLYVNALW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CBF0</accession>
    <name>A0A9Q1CBF0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Short-chain collagen C4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_12471</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CBF0"/>
    <dbReference type="OrthoDB" id="10062678at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005581">
        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051077">
        <property type="entry name" value="Ca-dependent_lectin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24024">
        <property type="entry name" value="PULMONARY SURFACTANT-ASSOCIATED PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24024:SF18">
        <property type="entry name" value="SHORT-CHAIN COLLAGEN C4-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0176">Collagen</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040458556" evidence="2">
        <location>
            <begin position="21"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="97"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8041604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="336" mass="35784" checksum="7D13682205175B35" modified="2023-09-13" version="1">MFPQVIVTLFVVGAIHPSCCFTTGDPPSDSVKTSNMKIRSLHSVPDIADEDDVRSVEVQSNHQRSSAIDYTDKIDEKNLLASKRVVASSCQSCPLCPSGPPGPAGPPGEKGKPGRDGRDGRDGRDAMYSAPVTNGSEGSGAHPPRPPPPTFNGSLPGVTYIRWGRKSCPEHSRLVYDGVMGGSYYNYKGSGSNYLCLPKNPKFGPNGGTSGGAFIYGVEYNTNLPGVTNIINSEAPCAVCQAPGRQNALVIPATNECTGKEWSLEYHGYMMSSHYSHNRAEFICVDGKAEGIPRSSYNNEDSSLFVVESRCSSSGGGLPCPPYANYLDLLCAVCTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CC39</accession>
    <name>A0A9Q1CC39_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Hyalin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_12759</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CC39"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003410">
        <property type="entry name" value="HYR_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24273">
        <property type="entry name" value="FI04643P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24273:SF32">
        <property type="entry name" value="HYALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02494">
        <property type="entry name" value="HYR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50825">
        <property type="entry name" value="HYR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="805"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="domain" description="HYR" evidence="4">
        <location>
            <begin position="257"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="HYR" evidence="4">
        <location>
            <begin position="520"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="domain" description="HYR" evidence="4">
        <location>
            <begin position="696"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="26"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="961"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="43"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="974"/>
            <end position="984"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8041839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1031" mass="114009" checksum="294A58163FA69B9F" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CCQ5</accession>
    <name>A0A9Q1CCQ5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GIY-YIG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_09765</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8042887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CCQ5"/>
    <dbReference type="OrthoDB" id="10004661at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="CDD" id="cd10442">
        <property type="entry name" value="GIY-YIG_PLEs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000305">
        <property type="entry name" value="GIY-YIG_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035901">
        <property type="entry name" value="GIY-YIG_endonuc_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21301">
        <property type="entry name" value="REVERSE TRANSCRIPTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21301:SF10">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82771">
        <property type="entry name" value="GIY-YIG endonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50164">
        <property type="entry name" value="GIY_YIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GIY-YIG" evidence="1">
        <location>
            <begin position="265"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8042887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="365" mass="41243" checksum="A4FA82641E745C49" modified="2023-09-13" version="1">MPRVPDSKNIGVVQAYLTSPLGSEGSAFLGNKKLRICLPRIDEGIGSRHSCFKKDRKMNRFSSPEVFLVKDKAFQDLQEYILKSGKVKVLRSTYEVGRYWICSDDQDFAKVSKQIISFFEQRQYPQRVLSNALKRIQGIDRASALAPKTDHTPTRRIPLVLSFHPSVTPIVRAIYRNVETLRHDPSTRDHFPDPPITAFRIEKNISKHLVRSSQPQAVVPDTPGTFPCNRGRCNTCPVVSYDKNLSIVGPNNNRFNVHQHFTCTSANVVYVLVCKRCNILYVGETKRRLADRVTEHLRSIKQNLPGFPVATHFNPPSTCSIRDLMVSAAISCRGSDHDRLAAENRLIMKLGTLSPHGLNVRLELL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CEX5</accession>
    <name>A0A9Q1CEX5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TMEM248/TMEM219 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_11445</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8044081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CEX5"/>
    <dbReference type="OrthoDB" id="6329605at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039493">
        <property type="entry name" value="TMEM248/TMEM219"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039587">
        <property type="entry name" value="TMEM248/TMEM219_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16002:SF4">
        <property type="entry name" value="TMEM248_TMEM219 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16002">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 248-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14940">
        <property type="entry name" value="TMEM219"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="165"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="TMEM248/TMEM219" evidence="6">
        <location>
            <begin position="9"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF14940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8044081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="208" mass="23437" checksum="016BD4386F0B0C46" modified="2023-09-13" version="1">MALKPVDNLRGFAYQRPPFVAFLICLLLFAIALISLGLYMQNHKVQNYETEDWNNFFKSFSDQRFCIPLNDSNISQPPSSPPLYSAGAPTTQDPGTDEFDISDIVGTKNVSVSVNLDILFPGEDVVAYGNIALTFDLQQAIPDVKCKDQSCTAWKEEKSIINLHLMYTSYFLFIMLITSVFYALIKGPPFKQRVLHENGSHRKPMTLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CG15</accession>
    <name>A0A9Q1CG15_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Merlin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_11071</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CG15"/>
    <dbReference type="OrthoDB" id="6018897at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13194">
        <property type="entry name" value="FERM_C_ERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000002">
        <property type="entry name" value="radixin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000013">
        <property type="entry name" value="radixin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.360.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011174">
        <property type="entry name" value="ERM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011259">
        <property type="entry name" value="ERM_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041789">
        <property type="entry name" value="ERM_FERM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046810">
        <property type="entry name" value="ERM_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000798">
        <property type="entry name" value="Ez/rad/moesin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019747">
        <property type="entry name" value="FERM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018979">
        <property type="entry name" value="FERM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018980">
        <property type="entry name" value="FERM_PH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008954">
        <property type="entry name" value="Moesin_tail_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23281">
        <property type="entry name" value="MERLIN/MOESIN/EZRIN/RADIXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00769">
        <property type="entry name" value="ERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20492">
        <property type="entry name" value="ERM_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09380">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00373">
        <property type="entry name" value="FERM_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09379">
        <property type="entry name" value="FERM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002305">
        <property type="entry name" value="ERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00935">
        <property type="entry name" value="BAND41"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00661">
        <property type="entry name" value="ERMFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01196">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48678">
        <property type="entry name" value="Moesin tail domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00660">
        <property type="entry name" value="FERM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00661">
        <property type="entry name" value="FERM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FERM" evidence="6">
        <location>
            <begin position="11"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="308"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="526"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="66"/>
            <end position="69"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol)</name>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="284"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol)</name>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR002305-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8043804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="588" mass="68722" checksum="230EE5C88E6D2A28" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CGL4</accession>
    <name>A0A9Q1CGL4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_07369</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CGL4"/>
    <dbReference type="OrthoDB" id="5404895at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1610.10">
        <property type="entry name" value="CV3147-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008040">
        <property type="entry name" value="Hydant_A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045079">
        <property type="entry name" value="Oxoprolinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048350">
        <property type="entry name" value="S-Me-THD-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010318">
        <property type="entry name" value="S-Me-THD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027479">
        <property type="entry name" value="S-Me-THD_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365">
        <property type="entry name" value="5-OXOPROLINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365:SF10">
        <property type="entry name" value="HYDANTOINASE_OXOPROLINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05378">
        <property type="entry name" value="Hydant_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20906">
        <property type="entry name" value="S-Me-THD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06032">
        <property type="entry name" value="S-Me-THD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160991">
        <property type="entry name" value="CV3147-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydantoinase/oxoprolinase N-terminal" evidence="1">
        <location>
            <begin position="10"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="S-Me-THD N-terminal" evidence="2">
        <location>
            <begin position="143"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="S-Me-THD-like C-terminal" evidence="3">
        <location>
            <begin position="314"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF20906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8044586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="477" mass="51654" checksum="3378EBE4942FB668" modified="2023-09-13" version="1">MPCLLTEWSVSRLNIGTNYFINSLAQRSHLVPVSVVRLCGPATRALGPFDDFPEDLRKLISSKCFYVNGGYQVDGSVITDLDEKEIMDVIKEIKESNIRNVVISGVFSFMKTDQEKQNPGNITESTVPFVDESTGEWILSEYDVHCISIGTGILGSGGGGAPKIGLLRALTTLKKGRKIRVIHPERLKTKSFKNGKVGVPAFVGAPTVGNEKFASGREMVEALECIEKILKTDLLDENNKKQLDVKEDGNITYITHFDLTPLEGTQTNTRDIVALMCLEIGGSNATETLCAGADMGLPVLDCDGMGRAFPELQSSFQLGDAVLKAQKESSDPVESICEQGGGVHLITGKITNVSQETNGGFDRGITEIEGTGKYGDQEMIVEFQNENLIAKRRHTAEEKVVVTTPDIITVVGVDTGMAITNDIIKSGMHVAVIALLSAPQLRTKVALEVVGPKAFGYDTDYTPIGEYHSNCTIPLPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CGS5</accession>
    <name>A0A9Q1CGS5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_07143</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CGS5"/>
    <dbReference type="OrthoDB" id="1917519at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8044405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="96" mass="11478" checksum="8AC55C0366789304" modified="2023-09-13" version="1">MLRERTLLFLVEVKGHLRSPEVKNRKLCKQDISRFVMSRERTLLFLVEVKGHPGSPEVNLRKPRKRLVYRFVMLRERTLLFLVEVKGHLRSPEVKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CH09</accession>
    <name>A0A9Q1CH09_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Gamma-aminobutyric acid type B receptor subunit 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_07671</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CH09"/>
    <dbReference type="OrthoDB" id="2150267at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038039">
        <property type="term" value="C:G protein-coupled receptor heterodimeric complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004965">
        <property type="term" value="F:G protein-coupled GABA receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007214">
        <property type="term" value="P:gamma-aminobutyric acid signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15047">
        <property type="entry name" value="7tmC_GABA-B-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002455">
        <property type="entry name" value="GPCR3_GABA-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10519:SF20">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 156-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10519">
        <property type="entry name" value="GABA-B RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01177">
        <property type="entry name" value="GABAB1RECPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01176">
        <property type="entry name" value="GABABRECEPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="237"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="275"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="314"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="354"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="417"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="448"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="476"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="11">
        <location>
            <begin position="45"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="12">
        <location>
            <begin position="312"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="533"/>
            <end position="560"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8044811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="618" mass="69435" checksum="8270C04A5FBCA833" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CHS7</accession>
    <name>A0A9Q1CHS7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_08612</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CHS7"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8045577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="123" mass="14040" checksum="C8AF31364C67DF61" modified="2023-09-13" version="1">MPHIERKNPIVFGGGQRSPGVTGGQTLKTLLTQYLEVGTLDEFHTLYVDALWKEEEPYHFWWRSKVIWGQRGSNPENLVNTISQEQKLQQFSNLVHRCPILRGRTLLFLVGVKGRLGSQGVKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CHV1</accession>
    <name>A0A9Q1CHV1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_07629</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CHV1"/>
    <dbReference type="OrthoDB" id="6502630at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8044779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="81" mass="9120" checksum="6E67C358C585FD83" modified="2023-09-13" version="1">MHYGKRKNPIVFDGGQRSSGVTGGRMLKTLLTRYLKMRYGKRKNPIVFDGGQRSSGVTGGRMLKTLLTRYLKVGSLDKFHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CK94</accession>
    <name>A0A9Q1CK94_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Glutamate dehydrogenase 1, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_05559</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the Glu/Leu/Phe/Val dehydrogenases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CK94"/>
    <dbReference type="OrthoDB" id="6413352at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016639">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006095">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006096">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006097">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016211">
        <property type="entry name" value="Glu/Phe/Leu/Val/Trp_DH_bac/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42722">
        <property type="entry name" value="LEUCINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42722:SF1">
        <property type="entry name" value="VALINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00208">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02812">
        <property type="entry name" value="ELFV_dehydrog_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00082">
        <property type="entry name" value="GLFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00839">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="2 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamate/phenylalanine/leucine/valine/L-tryptophan dehydrogenase C-terminal" evidence="5">
        <location>
            <begin position="194"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8046773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="437" mass="47460" checksum="CC4FA62AAA6B9522" modified="2023-09-13" version="1">MLPMSPRSFVNNLKERGINRCYWVLDISSGSVTCSHPDELAGAGSIFVKEDRDYNGHEGVFLQLGKRTNTLLGAFIWRSNRGQACGGIRLWDYDCMEEYLRDGLRLAQGMGLKSALAGLWSGGGKGVIQMPDGGKEKDPSFRTDMFHDYGEFLSSLNGCYVAAEDVGLNVNDLDDVHTFTRYTTCISESKGGSGNPSTATGKGVICAMEGALDYLDMGTLAGKSIAIQGAGNVATVIVEGLLQRNVGRILVTDCNQDRIDAMRVKFSQNLDKLILEKVDVGDNSILSAPCDVLSPCALGNILNPTTIPGIKAKIVCGAANNQLGCPADSQLLSDHGIVYVVDFLANRMGIVNCANETYGRLTNDSAIERHFGRDWENSVFCMTKATLEKADKDGITTHEAANQIADKLSLKLHPVWPRRGQEIISSLVKEQWHLQSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CLJ9</accession>
    <name>A0A9Q1CLJ9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">5-hydroxytryptamine receptor 2B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_06488</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CLJ9"/>
    <dbReference type="OrthoDB" id="6117944at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00637">
        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24228">
        <property type="entry name" value="B2 BRADYKININ RECEPTOR/ANGIOTENSIN II RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24228:SF59">
        <property type="entry name" value="NEUROPEPTIDE RECEPTOR 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="30"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="70"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="150"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="196"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="277"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="304"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="50"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8047478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="341" mass="38440" checksum="ABB2CE3797E87789" modified="2023-09-13" version="1">MNQSTKFSTSVYVTTVDQEYRRYLPVPLPYAVTFLFIICCVVGFLVGSVGNILVILAIARKRRLRTTANAFLASLAVADFGTCILVLPILSVAFVSLESVITNQFACRFFYHTTFTFSPVSIQHLILIAANRFILINKSSRTYVKCFNKVSISAVIFLIWTLNISFNIAVAFSSYGTMTFFGKEYCYVSQKNTIGTLFWAITLLYTFISSFVIIPMFYIFTFHTVHKSHVRVSETPSVATVTFNPKLSKSKASATGTQRKNRRLLSSSEVKLTKVNFFIFMTNVLFLSPMFFALAITGNDMTHGIFLLVLFPVGMNSVTNPLVYCGFNRNFRHAFKTLLKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CMC8</accession>
    <name>A0A9Q1CMC8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">IgGFc-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_06307</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CMC8"/>
    <dbReference type="OrthoDB" id="5945029at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="CDD" id="cd19941">
        <property type="entry name" value="TIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025615">
        <property type="entry name" value="TILa_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040941">
        <property type="entry name" value="GGGWT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12714">
        <property type="entry name" value="TILa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040197343" evidence="1">
        <location>
            <begin position="23"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="2">
        <location>
            <begin position="225"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8047330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="303" mass="33267" checksum="4CC805D13141B586" modified="2023-09-13" version="1">MASRNVLLFVIMTGTCIKMTESMGLCEFEGKFPCADESECPKYICIGVRRGNPAKDNENETSTPQHISTHTTTIDLTTNVKLSTNRNTTVQLDGYSTADPLPTNPVCPLNMVYTNCSCQGTCEDPSGCHNNCSESKMCVCPNGFLMRGDDCIPEMMCGCFIDGYGVLRKDGFYVNSTCSERCTCFEDILSCEEDFKCDSNAICERRDGVRDCYCNTGFAGDGQICISTSTFSDCYEIFSAGITSDGVYTVTPNGWTGAPFEVYCNMSHGGGWTVSSSMSNYGRDNFFKESEIGRPFPIFTFLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CMU3</accession>
    <name>A0A9Q1CMU3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_00352</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CMU3"/>
    <dbReference type="OrthoDB" id="10035376at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008528">
        <property type="term" value="F:G protein-coupled peptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016500">
        <property type="term" value="F:protein-hormone receptor activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009755">
        <property type="term" value="P:hormone-mediated signaling pathway"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002131">
        <property type="entry name" value="Gphrmn_rcpt_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24372:SF77">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24372">
        <property type="entry name" value="GLYCOPROTEIN HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00373">
        <property type="entry name" value="GLYCHORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
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    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="215"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="258"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="340"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="384"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="439"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="471"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="227"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8048161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="663" mass="75386" checksum="C9FBD59B152824F1" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CN87</accession>
    <name>A0A9Q1CN87_HOLLE</name>
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        <recommendedName>
            <fullName evidence="3">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_00581</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CN87"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF13">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="3">
        <location>
            <begin position="841"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="307"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="574"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="674"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="71"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="307"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="322"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="349"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="574"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="618"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="643"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="690"/>
            <end position="714"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8048318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1053" mass="119445" checksum="F764C0BC3BCF305E" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CTF0</accession>
    <name>A0A9Q1CTF0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Transcriptional regulator MNL1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_04090</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CTF0"/>
    <dbReference type="OrthoDB" id="5988713at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050527">
        <property type="entry name" value="Snail/Krueppel_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24388:SF54">
        <property type="entry name" value="PROTEIN ESCARGOT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24388">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="3"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="32"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="74"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="76"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8050766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="505" mass="58423" checksum="A1D44B672DE07B55" modified="2023-09-13" version="1">MACKCTLCEKLFHRLDHLKRHRDTVHTKGSNLQCDICTKLFSRKDNLRRHIKTHATVHKERCDQVNNALSNLSSSTAMQSDNSELPDQTLPASATGLPLTDENSLQKPLKDGKRKRENSRDTEEIATSSKRIKTLESVSYTLHEDPLKPPSNIDEYVSIFRFPEGVDCPTEDVNKLCRKHWNTIRTKHRRQNKLRDTYNFRLTKTPPEELLCQTKMIFEDQKFVFKLNVGLGFFLRNTETCEIRYYYPSNNSKLFSEPFLITNTSTLEHFNKALLAQDLLEHARLQRPNSKWVVESITNVTYFVYKIPDHPIGCVTTDLPDFVRNNKALITLERNRKTGERYQDNLCLFRCLALFHGAHPNGLEKETKRLCRQYFRQDPNNFNGIPLKELSSFEDTFKVNVFVYELIRHDVNDSDLTSEATTDSQQCNSTGANVSARLVRRSLARYDNTLYVNLSGNHFSYISNIKMYTKTHGQKAPGPYIRYKSLFVSRNATVTTHSFPSGKCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1GXT0</accession>
    <name>A0A9Q1GXT0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ZU5 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_29710</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8027689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1GXT0"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005042">
        <property type="term" value="F:netrin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.220.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037936">
        <property type="entry name" value="UNC5A-D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000906">
        <property type="entry name" value="ZU5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12582">
        <property type="entry name" value="NETRIN RECEPTOR UNC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12582:SF41">
        <property type="entry name" value="UNC5C-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00791">
        <property type="entry name" value="ZU5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51145">
        <property type="entry name" value="ZU5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ZU5" evidence="1">
        <location>
            <begin position="1"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8027689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="196" mass="22311" checksum="2CD6803729357FF7" modified="2023-09-13" version="1">METSAVVDNHGCVLRIPGTGVALNIPPNAFYDEGEEHNVTLRILRHQFIDESSFEDLSTVMVEILPNKLTLLEDARLILPHCLEIQNPEECLVQVFESHHDPETEPLWKDISQSVSYTLRESFCEIFLKSFCWVKYKLPTGERVKAKKIVVYATGNVPAPFQGHKCNRATIDVGYHPDLPGKDKVHLAISFFFLYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1GZY7</accession>
    <name>A0A9Q1GZY7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ragulator complex protein LAMTOR1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Late endosomal/lysosomal adaptor and MAPK and MTOR activator 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_30684</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Late endosome membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Lysosome membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the LAMTOR1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1GZY7"/>
    <dbReference type="OrthoDB" id="5562028at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071986">
        <property type="term" value="C:Ragulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071230">
        <property type="term" value="P:cellular response to amino acid stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042632">
        <property type="term" value="P:cholesterol homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007040">
        <property type="term" value="P:lysosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043410">
        <property type="term" value="P:positive regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032008">
        <property type="term" value="P:positive regulation of TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001919">
        <property type="term" value="P:regulation of receptor recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028209">
        <property type="entry name" value="LAMTOR1/MEH1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13401">
        <property type="entry name" value="RAGULATOR COMPLEX PROTEIN LAMTOR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13401:SF2">
        <property type="entry name" value="RAGULATOR COMPLEX PROTEIN LAMTOR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15454">
        <property type="entry name" value="LAMTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01262">
        <property type="entry name" value="LAMTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="9" id="KW-0458">Lysosome</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0519">Myristate</keyword>
    <keyword evidence="8" id="KW-0564">Palmitate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00032695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8028453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="168" mass="18746" checksum="6158A7ED3786C445" modified="2023-09-13" version="1">MGGCCGKDNDKPSVEPESSETSRLLGPQNGRTPDKPMYGDGGERHPNLLKRGDEQSALDQILHNTANNIIDVAALDSRRMEQQEYLDRARQYGSRVSNLVPNASRLQSHMLQLHNGTSMPYSVLSAEPISLHDINLINHTAEKFWNACEGIKVNHKEDLVVNFEIPEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H1V8</accession>
    <name>A0A9Q1H1V8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Guanine nucleotide-binding protein subunit beta-5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_28975</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat G protein beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H1V8"/>
    <dbReference type="OrthoDB" id="10255630at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000020">
        <property type="entry name" value="Guanine nucleotide-binding protein beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016346">
        <property type="entry name" value="G-protein_beta_1-5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001632">
        <property type="entry name" value="WD40_G-protein_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19850">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN BETA G PROTEIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25391">
        <property type="entry name" value="WD40_Gbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002394">
        <property type="entry name" value="GN-bd_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00319">
        <property type="entry name" value="GPROTEINB"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="65"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="153"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="195"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="239"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="325"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8029550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="357" mass="39299" checksum="BB61F404D72571DF" modified="2023-09-13" version="1">MASSGKNESTPKAKEDTIPSLTREAANLKEKLEEERRILNDVELSVVASRLDPLPPISLKPRRILKGHQGKVLCLDWARDQRHMVTSSQDGKVIVWDAFTNTKEHSITMPTTWVMACAYAPSGTMVACGGLDNKCSVYQLAQEEDLSSKKRAVAMHTSYVSCSTFLNSDQQILTGSGDSTCALWDVESGQLLQSFHGHNADVMGIDLSPSEACNTFVSGGCDKQALVWDMRTGQCVQSFEGHESDLNAVRFLPSGDAFATASDDASCRLYDLRADREINCYAKESIIFGATSLDFSLSGRMLFVGYNDYSVNIWDTLKGVRLTMLFGHENRVSCLRVSPDGTALCTGSWDYTLRVWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H2G9</accession>
    <name>A0A9Q1H2G9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNA-directed DNA polymerase from mobile element jockey</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_27076</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H2G9"/>
    <dbReference type="OrthoDB" id="6287244at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46670">
        <property type="entry name" value="ENDO/EXONUCLEASE/PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46670:SF3">
        <property type="entry name" value="ENDONUCLEASE_EXONUCLEASE_PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8030613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="169" mass="19300" checksum="56CF583E44479F86" modified="2023-09-13" version="1">MISNDRPNPFHPVKRFVYSRNQLYSIRRRQCVPPLQQDTIDTLKAHTIWRFRGNRRGSHVRRSCYATSQLRNPNALGSEDRQTVHVAVPPSPLLLDFGLINARSVKNKDVVLVDYIIDHNLDLLGITETWLSTGNQDNVDLGRLKTAGYEFRHVPRLTGQGGGLALIYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H2X9</accession>
    <name>A0A9Q1H2X9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_26597</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H2X9"/>
    <dbReference type="OrthoDB" id="6417614at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8030241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="106" mass="12259" checksum="D0EA877EAD483C7E" modified="2023-09-13" version="1">MSRRVTEQAPFLHVLTRGTTQQQSALLKRLHNALLICSCECALNILKCKVKLTPSRKLHLQRHRAKLRKLVDRKVSLSQKRKVFRQKMGGHCVRSILLPIIKQLTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H447</accession>
    <name>A0A9Q1H447_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Natterin-4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_25624</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H447"/>
    <dbReference type="OrthoDB" id="428159at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.15.10">
        <property type="entry name" value="Proaerolysin, chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053237">
        <property type="entry name" value="Natterin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39244:SF5">
        <property type="entry name" value="NATTERIN-3-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39244">
        <property type="entry name" value="NATTERIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56973">
        <property type="entry name" value="Aerolisin/ETX pore-forming domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040453519" evidence="1">
        <location>
            <begin position="21"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8032173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="362" mass="41465" checksum="9AE66DD2BD32BEFD" modified="2023-09-13" version="1">MAKILSSILLLFAVFHTFEGRKHTVIHNEDDLIGSCTKTCDTLPDRSDQFDCEWIDNCEYNRIGWTRGFVKCNWCRCDCTTPDDIPAKEEISREKIILTELDLWNTCEETCSYSGLQRTDYKGCSEIRDCQWNSNGWISGFVRCDYCKCDCIKKGYPNNYRIENVVYQMNDLDTSLGDSVNVGEVTVENHGNDTVTTSYTIKMVYTTETMWSSSAGIIMGSEVKITFDELIIEEEVTIGGEVSYDYTWGETRTETVENYITAQPTSPPHSIRQVWAQGSRYTLDVPYTADLVTVYDDGTEVTSQISGTLNQASVSKFHVVYGEIHDLPEEESVESDIEWLDEEKLRSEHHQCIIAYFFKPRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H5P2</accession>
    <name>A0A9Q1H5P2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_23616</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H5P2"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8033390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="53" mass="5812" checksum="D19FE576A7A85686" modified="2023-09-13" version="1">MAAETIKSRIDHVDGGLKCVATGNPGRFCLIDPRCSVTRSARRRFVSPTYSTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H6L5</accession>
    <name>A0A9Q1H6L5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Helix-turn-helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_21123</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H6L5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058912">
        <property type="entry name" value="HTH_animal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21301:SF12">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21301">
        <property type="entry name" value="REVERSE TRANSCRIPTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26215">
        <property type="entry name" value="HTH_animal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helix-turn-helix" evidence="1">
        <location>
            <begin position="67"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF26215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8034336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="306" mass="34911" checksum="715240783F52DD6C" modified="2023-09-13" version="1">MGNYQRNSGTRQDLESFIQFVGSFCPFLKFTHCISDTSVVFLDLQLSICDRKIKSNLHFKPTDSHNYLLYPSNHPKSCTKSIPYSQLLRARRICSEDSDFSAASKSILSFFEKRQYPTKILTGALHRIQGIDRAEALSKKSNTSTNLRIPLVISHHPSVRRPIIRAIYRNIETLRKDPSTRDLFPEPPNYGLQSLKRTFSKHYCTSTNLVYILVCGRCNCLYVEKPNAAWADRITEHLRSIKQNLQGYPVATHFNPPSPCSIKDLAVTAALMCRGSDRDRQTAENRLIMKLGTLSPHGLNIRMDLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H859</accession>
    <name>A0A9Q1H859_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_20682</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the histone deacetylase HD2 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H859"/>
    <dbReference type="OrthoDB" id="8113227at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000702">
        <property type="entry name" value="Transcription factor E4F1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000744">
        <property type="entry name" value="zinc finger E-box-binding homeobox 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000358">
        <property type="entry name" value="zinc finger protein 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000902">
        <property type="entry name" value="Zinc finger protein 445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000663">
        <property type="entry name" value="Zinc finger protein 45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000110">
        <property type="entry name" value="Zinc finger protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.340">
        <property type="entry name" value="Nucleoplasmin core domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041232">
        <property type="entry name" value="NPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050331">
        <property type="entry name" value="Zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515:SF66">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17800">
        <property type="entry name" value="NPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="265"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="293"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="321"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="349"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="377"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="410"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="438"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="109"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="193"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="125"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="209"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8036644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="516" mass="57806" checksum="CED43AAA3E272842" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H8T7</accession>
    <name>A0A9Q1H8T7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_20548</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H8T7"/>
    <dbReference type="OrthoDB" id="8939918at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8036541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="60" mass="6702" checksum="607970FA2AC37FEA" modified="2023-09-13" version="1">MDEATCKLAVQALIFSRIYYGNALLLGGTEHDLTLLKRLQNTAARLILLVERELPCTTLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H982</accession>
    <name>A0A9Q1H982_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Leucine-rich repeats and immunoglobulin-like domains protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_18582</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H982"/>
    <dbReference type="OrthoDB" id="4691307at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45617">
        <property type="entry name" value="LEUCINE RICH REPEAT FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8037694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="266" mass="30663" checksum="2996C1D6D06F75D3" modified="2023-09-13" version="1">MECKQVEYFINVIFILIIYKIRISSSSCKCSGSTIDCSRLKLTRMTWSNREECNETLSTEKVVLDFSHNQISLTGERILPLEDVETIDLSYNNIDQLLGFGQSTLHSYLSNLVLSHNSIKSIKENDLQSLSSLKLLDVSYNRILCIEEGTFSFLFRLEYLDLSWNHLTTFAMHCPDSIEVLYFNNNRLHTFILEGTCNFKELDLTSNNFSTFPETDDSWKGNIYFGGNPEVCKSLEEREDVYTNIQCEILVKFKAGNLKGRMVRSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HAR0</accession>
    <name>A0A9Q1HAR0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="1">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_15896</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="1"/>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HAR0"/>
    <dbReference type="OrthoDB" id="77911at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056598">
        <property type="entry name" value="FKBP-15_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44927">
        <property type="entry name" value="FK506-BINDING PROTEIN 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44927:SF1">
        <property type="entry name" value="FK506-BINDING PROTEIN 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23649">
        <property type="entry name" value="FKBP15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase FKBP-type" evidence="4">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="44"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="130"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="764"/>
            <end position="1142"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="313"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="670"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="159"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="169"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="205"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="224"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="770"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="813"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="841"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="873"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="898"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="909"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="996"/>
            <end position="1012"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1019"/>
            <end position="1030"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1101"/>
            <end position="1113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1132"/>
            <end position="1142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8038461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1142" mass="126964" checksum="0EB769929132A986" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HB39</accession>
    <name>A0A9Q1HB39_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Versican core protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_09525</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8042702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HB39"/>
    <dbReference type="OrthoDB" id="5989315at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005112">
        <property type="term" value="F:Notch binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000327">
        <property type="entry name" value="neurogenic locus notch homolog protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000434">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12916">
        <property type="entry name" value="CYTOCHROME C OXIDASE POLYPEPTIDE VIC-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12916:SF9">
        <property type="entry name" value="NEUROGENIC LOCUS NOTCH HOMOLOG PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00010">
        <property type="entry name" value="EGFBLOOD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1 6" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="EGF-like" evidence="7">
        <location>
            <begin position="10"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="7">
        <location>
            <begin position="48"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="36"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="74"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8042702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="94" mass="10395" checksum="6648EC920731CD51" modified="2023-09-13" version="1">MLYGFYQDSDTDECYSNPCNFGGTCVGIGNEFKCECAPGYEGDFCETDTDECYSNPCQFGGTCSDFVNKFKCDCAPGYEGDFCERASRKLQVPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HCA4</accession>
    <name>A0A9Q1HCA4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_14389</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the CFAP144 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HCA4"/>
    <dbReference type="OrthoDB" id="446290at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097546">
        <property type="term" value="C:ciliary base"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029214">
        <property type="entry name" value="CFAP144"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33865">
        <property type="entry name" value="PROTEIN FAM183B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33865:SF3">
        <property type="entry name" value="PROTEIN FAM183B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14886">
        <property type="entry name" value="FAM183"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8040168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="138" mass="16398" checksum="1B32F087E3F4F35F" modified="2023-09-13" version="1">MAAPAKTTEKEPVNIVHQNAILCETIKKENQCQRLYTNYGVNPFKKMYTLTGKPNSQHDSDEGEEDEYFLEIIKRSYQVPEKKFEFPMTEAQEVGWYNEPLLPQDRSDRRLMFPRKNSEITKYMDASWKQKEQQENLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HCN8</accession>
    <name>A0A9Q1HCN8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Ras guanyl-releasing protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_07267</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RASGRP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HCN8"/>
    <dbReference type="OrthoDB" id="74314at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20808">
        <property type="entry name" value="C1_RASGRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00155">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06224">
        <property type="entry name" value="REM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.840.10:FF:000003">
        <property type="entry name" value="Ras guanyl-releasing protein 3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.840.10">
        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.870.10">
        <property type="entry name" value="Son of sevenless (SoS) protein Chain: S domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020454">
        <property type="entry name" value="DAG/PE-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008937">
        <property type="entry name" value="Ras-like_GEF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000651">
        <property type="entry name" value="Ras-like_Gua-exchang_fac_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019804">
        <property type="entry name" value="Ras_G-nucl-exch_fac_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023578">
        <property type="entry name" value="Ras_GEF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001895">
        <property type="entry name" value="RASGEF_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036964">
        <property type="entry name" value="RASGEF_cat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113:SF252">
        <property type="entry name" value="RAS GUANYL-RELEASING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00036">
        <property type="entry name" value="EF-hand_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13202">
        <property type="entry name" value="EF-hand_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00617">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00618">
        <property type="entry name" value="RasGEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00008">
        <property type="entry name" value="DAGPEDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00147">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00229">
        <property type="entry name" value="RasGEFN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48366">
        <property type="entry name" value="Ras GEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00720">
        <property type="entry name" value="RASGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50009">
        <property type="entry name" value="RASGEF_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50212">
        <property type="entry name" value="RASGEF_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="2 7" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="N-terminal Ras-GEF" evidence="11">
        <location>
            <begin position="30"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GEF" evidence="9">
        <location>
            <begin position="197"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="12">
        <location>
            <begin position="462"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="12">
        <location>
            <begin position="500"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="10">
        <location>
            <begin position="533"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="633"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="635"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="675"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="726"/>
            <end position="736"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8044502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="763" mass="86283" checksum="174ACA98B597FF84" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HD55</accession>
    <name>A0A9Q1HD55_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">TRP C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_15244</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HD55"/>
    <dbReference type="OrthoDB" id="10062419at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="209"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="255"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="319"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="359"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="386"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8040831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="467" mass="53747" checksum="F56CCD7DB7E0B7D5" modified="2023-09-13" version="1">MNCSNDYVTLKSGYYWNWSYTDINIYKRFILNLQTFNDSYDNETVNFTEPFPMTIECSQRFKCVNDNGDIEGNCAIGYSGWMCTACQDGFFPILGYCHSCPKFWVFFLEVLGIGCVCICFAVYLVYSYRREQSGEHRSIVDIALARGKILLGFYQIMGEFWNSLHTVYWSEVFKQVSQWLGLLQFNISSILIKPSCFMQQLTLTPYTEFIVGISTPLMLLFITAVANLLGRGFIIRSRGGGSSSLSGNDTRFRRIQDKFLSVTLLILFVTYTSTCNDTTLGCESGISNPKWIRFLCENYKDRFWFWEIIELVRKMSQTFVVILFGWSSSLSITVSLTLAVIFLCLHTSYSPMRDKFEHYLQLASLWAIFLNMLVAAVRVPDRLNSVYVQTAMTLMVILLNLSVVAIVIGKTLVSLVKFMYQSCSYRCIARCFPTWYRKINGVTSRTFVELEDTYPTEQTPLINMLSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HDC8</accession>
    <name>A0A9Q1HDC8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tyr recombinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_11755</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HDC8"/>
    <dbReference type="OrthoDB" id="10064229at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="entry name" value="Intergrase catalytic core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35617:SF3">
        <property type="entry name" value="CORE-BINDING (CB) DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35617">
        <property type="entry name" value="PHAGE_INTEGRASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyr recombinase" evidence="2">
        <location>
            <begin position="110"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8041056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="322" mass="36880" checksum="45F609BB97B63254" modified="2023-09-13" version="1">MLQIIRESIKDYGFSAKTADIILSSRRESTTKQYSITLRNGWIFVINKRQIHCMHSNPRVRDFFTSLYENIGYSALNTARAAISSLVSLTDGYTVGNHPLITRYFKGVFNLKPRYNQTWDVRLVLNYLRKLSLANKLGLKDLTLKTLMLLALVSSQRQQTLHKLDVRKMELKVNKVIFFVEDLLKHNRPGNTGLKLEFCSYPPDRRLCIHTYLMEYLKRTKKCRGRETSLLVSYGKPFSKVSTSTIARWLRTVMHNAGVDVTVFKPHSIRSATVSRASAANVPVLDILSHAGWKNEQTFQRFYNKPVGKDCKQSVATAILGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HDZ2</accession>
    <name>A0A9Q1HDZ2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Exportin-5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_09185</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the exportin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8046032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HDZ2"/>
    <dbReference type="OrthoDB" id="2215036at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042565">
        <property type="term" value="C:RNA nuclear export complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005049">
        <property type="term" value="F:nuclear export signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006611">
        <property type="term" value="P:protein export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006405">
        <property type="term" value="P:RNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013598">
        <property type="entry name" value="Exportin-1/Importin-b-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045478">
        <property type="entry name" value="Exportin-5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045065">
        <property type="entry name" value="XPO1/5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11223">
        <property type="entry name" value="EXPORTIN 1/5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11223:SF3">
        <property type="entry name" value="EXPORTIN-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19273">
        <property type="entry name" value="Exportin-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08389">
        <property type="entry name" value="Xpo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="2">
        <location>
            <begin position="57"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8046032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1188" mass="134403" checksum="3B39B7F362B32B64" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HDZ6</accession>
    <name>A0A9Q1HDZ6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_12576</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HDZ6"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8041686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="88" mass="9596" checksum="021DB46BDB5F8DAF" modified="2023-09-13" version="1">MRYGERKNPIIFGGGERSSGVTGGQTLKTSLTRYLKMRYGERKNPIIFGGGGRSSGVTGGQTLKTPLTRYLKKGSSDGSQTWYIDAPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HEM3</accession>
    <name>A0A9Q1HEM3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_13335</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8042313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HEM3"/>
    <dbReference type="OrthoDB" id="1917519at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8042313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="126" mass="14696" checksum="4706A33E547508B3" modified="2023-09-13" version="1">MSRRRSLLFLVEVKGHLRSPQVNLRKPCKRLVNTISQDSNDRHFSYLVYRFVMLRGRTLLFLVEVKGHLRSPEVKRQKPRKWLVNTISQVSNDRHFFIFCIQICHVEGKTPVVFGGGQRSFEATAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HFE0</accession>
    <name>A0A9Q1HFE0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">SRCR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_09976</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HFE0"/>
    <dbReference type="OrthoDB" id="536948at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000007">
        <property type="entry name" value="Soluble scavenger receptor cysteine-rich domain-containing protein SSC5D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48071:SF18">
        <property type="entry name" value="DELETED IN MALIGNANT BRAIN TUMORS 1 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48071">
        <property type="entry name" value="SRCR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00530">
        <property type="entry name" value="SRCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00258">
        <property type="entry name" value="SPERACTRCPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00420">
        <property type="entry name" value="SRCR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040217143" description="SRCR domain-containing protein" evidence="7">
        <location>
            <begin position="18"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="8">
        <location>
            <begin position="24"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="49"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="62"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="93"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8043048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="131" mass="14424" checksum="00F29772251980C4" modified="2023-09-13" version="1">MSHVLTLSIISVISVDSGSVIQDVRLVNGTSQSSGRIEVLHKGEWGTVCEDGWNLTDSNVVCRQLGYPTALETYRHAFFGEGSGFIWSNELQCIGNESFLSECKQDESRDPVCNHQQDVGVRCGSKRINVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HG08</accession>
    <name>A0A9Q1HG08_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_10863</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HG08"/>
    <dbReference type="OrthoDB" id="6773122at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8043666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="99" mass="11759" checksum="1B3F9C7CF5AB1D96" modified="2023-09-13" version="1">MAVYMAFGLLEWENQRARRRERVFRDRTNPLDLWTDQQMYAKYRFSRLACINIIDMLQDELEHRTRRNHALPPSLQVFIALRFFGHGSVTDDSGAEPMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HGA9</accession>
    <name>A0A9Q1HGA9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">small monomeric GTPase</fullName>
            <ecNumber evidence="5">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">HOLleu_07704</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">Nanhai2018</strain>
            <tissue evidence="19">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Early endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endosome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="5"/>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HGA9"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032482">
        <property type="term" value="P:Rab protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04113">
        <property type="entry name" value="Rab4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000280">
        <property type="entry name" value="Putative ras-related protein Rab-4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041819">
        <property type="entry name" value="Rab4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0967">Endosome</keyword>
    <keyword evidence="14" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="16" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0488">Methylation</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-0636">Prenylation</keyword>
    <keyword evidence="13" id="KW-0653">Protein transport</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAJ8044835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="222" mass="24712" checksum="35E38CFFFB1F5476" modified="2023-09-13" version="1">MTDKAETEQLVKMSETYDFLFKFLVIGSAGTGKSCILHQFIENKFKQDSNHTIGVEFGSKIVNAGGKSVKLQIWDTAGQERFRSVTRSYYRGAAGALLVYDITSRETYNALSKWLTDARTLASPNIVIILCGNKKDLDADREVTFLEASRFAQENEMMFMETSALTGENVEEAFLRCARSILTKIESGELDPERMGSGIQYGDSSLRRVARTPSRAPSKCAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HHY3</accession>
    <name>A0A9Q1HHY3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_08623</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HHY3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005819">
        <property type="entry name" value="H1/H5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00624">
        <property type="entry name" value="HISTONEH5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8045586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="207" mass="26470" checksum="802BB750BF586CBB" modified="2023-09-13" version="1">MVHIGHSLFDHKTKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKEEEEEEEGRRRRKEEEEEEEEKKKKKKKKKKKKRRRRKKKKKKEKKKKKKKKKKKKKKKKKNNKNKKKKKKKNNKKKKKKKKKKKKNKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKNRKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HIP2</accession>
    <name>A0A9Q1HIP2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_00405</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HIP2"/>
    <dbReference type="OrthoDB" id="8961218at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8048194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="94" mass="10843" checksum="0B000C4F9A1A6732" modified="2023-09-13" version="1">MATALKFLSFNARGLRQSKKRGQLFAFIHRRNPDISIIQESHSCINDESYLQHEWGGKVLFSHGSNTSRGVCILIKPNLDFEVVKLSLWKIYYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HIU5</accession>
    <name>A0A9Q1HIU5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HIN-200 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_05125</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HIU5"/>
    <dbReference type="OrthoDB" id="6121668at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004021">
        <property type="entry name" value="HIN200/IF120x"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02760">
        <property type="entry name" value="HIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HIN-200" evidence="1">
        <location>
            <begin position="139"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8046453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="250" mass="28233" checksum="A67EC93AA55CE63C" modified="2023-09-13" version="1">MRDLFDMVRQLGIPKCFCSFSAADRRWHEIDEENSSPTRESKTATPCLFAKGRIQKNREKYHSNILRLHSPTTDKECTPVVAPWTKYYSRMMLNHHTTGNNAIFGNAAILAVGDFYQLQPIATVDILNISTTNPYNPPIKVLLATKSEKIIYKKEETDKEMMYLALCDPTGHIKITLNDLSKVETFDNGSTIIVRNYLVTNDRTIGITSNSQIFKSSPLQVPDAVMQQAVCSVRPPTTPPIPLKKSIHHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HIV4</accession>
    <name>A0A9Q1HIV4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_05166</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HIV4"/>
    <dbReference type="OrthoDB" id="6287527at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8046483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="76" mass="8535" checksum="9D06BB098681A892" modified="2023-09-13" version="1">MESSDDSVMKVKFSTGDIKMTYEVKGLLLVDCGGTTHTVNDQSKFIRFDDDFKPADHYIELADGKRTNNIAMRKGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HJ04</accession>
    <name>A0A9Q1HJ04_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_06561</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HJ04"/>
    <dbReference type="OrthoDB" id="6334211at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="168"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="193"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="225"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8047535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="235" mass="24262" checksum="F0EEE135BE31B680" modified="2023-09-13" version="1">MTYLGIGSSATGSSEQYSGGDRVTSASGTHSEDDRATPASGTSSQSESGSLSRSPSQRPGASSVGSSVKTWSKPSGEPATKHSSSPGLRQREGSSRQMNSVERASYERRKSAAKAAAAGIIPKPLEQLINKPPGTAPGESQFDLGPEKPLRPKTVPSSLPYHDFYSASPDSSVPSIASDSHLSNISEAGGMSPSGSSVSNDSRPASAISFASDKPALPPINTSEQDSDPYNDPEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HK42</accession>
    <name>A0A9Q1HK42_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nucleoplasmin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_01167</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HK42"/>
    <dbReference type="OrthoDB" id="1902587at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.340">
        <property type="entry name" value="Nucleoplasmin core domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041232">
        <property type="entry name" value="NPL"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17800">
        <property type="entry name" value="NPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleoplasmin-like" evidence="1">
        <location>
            <begin position="2"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF17800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8048735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="86" mass="9625" checksum="49F8FCF675AC34BC" modified="2023-09-13" version="1">MTLHPGVEYCREVTKEEHLTLASLDCRPKMNKGVYHPLAHLVVKVNDCEHVLCYLEKGISHQQGLDFIFQEGDNLALSVEGTGKCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HL83</accession>
    <name>A0A9Q1HL83_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Zinc finger CCHC domain-containing protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_03191</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HL83"/>
    <dbReference type="OrthoDB" id="7608935at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002218">
        <property type="term" value="P:activation of innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042509">
        <property type="entry name" value="ZCCHC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22639">
        <property type="entry name" value="GAG-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22639:SF3">
        <property type="entry name" value="ZINC FINGER CCHC DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="198"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="260"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8050115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="322" mass="34763" checksum="B42F3513A7233590" modified="2023-09-13" version="1">MPGGRDSTIVKLEFSSGASVSPPQAFTVLKNKGIQVKEIEMLQALAARTTYDLKFRFDAARVKGVSCLKGAEVLTVTPYDRSVAVTVLYLNFEVDQRLVARVLSQYGTVSDMRWCKYGSGELAGIFNGKRQCRMELDRDIPSFLFIGGSKAHIRYFGQPRTCFKRGQEGQEARCCPNRRCGKCLQVGHDKAECPNEVRCNLCGEEGHVSGACPTSYSAHASADVEQAPEPSQAGPVYSTEELEEAAQDVEKDFLASKQCEGGTVAPKADSSPSSPPTPVEADRMAVEEDLQLSSDSESAMSECGGVCLRASSQGSTFRGGLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HLM9</accession>
    <name>A0A9Q1HLM9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_04040</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HLM9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8050730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="51" mass="5838" checksum="160E8A285D2C9B80" modified="2023-09-13" version="1">MVIIVLFPSLVETFHVFVLQKEAKMSITRVKFSHASRVCAHVKPIPLSCLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HLV4</accession>
    <name>A0A9Q1HLV4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Interferon-induced very large GTPase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_03504</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class dynamin-like GTPase superfamily. Very large inducible GTPase (VLIG) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1597724at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030383">
        <property type="entry name" value="G_VLIG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057365">
        <property type="entry name" value="URGCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052986">
        <property type="entry name" value="VLIG_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14819">
        <property type="entry name" value="GTP-BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14819:SF5">
        <property type="entry name" value="INTERFERON-INDUCED VERY LARGE GTPASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25496">
        <property type="entry name" value="URGCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25683">
        <property type="entry name" value="URGCP_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51717">
        <property type="entry name" value="G_VLIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VLIG-type G" evidence="3">
        <location>
            <begin position="618"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="412"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1509"/>
            <end position="1533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1509"/>
            <end position="1518"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8050341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1533" mass="175257" checksum="F2AE993122887A47" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HLV6</accession>
    <name>A0A9Q1HLV6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_03380</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HLV6"/>
    <dbReference type="OrthoDB" id="10056585at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37162:SF11">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37162">
        <property type="entry name" value="HAT FAMILY DIMERISATION DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="496"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8050248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="527" mass="60466" checksum="2A94EB8F9CD5856C" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HM19</accession>
    <name>A0A9Q1HM19_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_03903</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HM19"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8050626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="104" mass="11447" checksum="9A8C9B9E6F387FDD" modified="2023-09-13" version="1">MPRFEHIGKAKNKAISCTICDLSPPKLSKFSQRGGGHRPPPPGQRSQEPPDSKQTSGEPCNTSESSCCYAMTFPSSTPRIFNSECRECRLGQRSYLANAEMPKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HM38</accession>
    <name>A0A9Q1HM38_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_04023</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HM38"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8050716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="60" mass="6983" checksum="A9AFAAB0E774AC29" modified="2023-09-13" version="1">MLLHKLNSSGSPTFWLPSFEKQSNYFHSDRKCYTLGLSRARKAHRPSQNCPLQQRSIAYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YAW7</accession>
    <name>A0A9Q0YAW7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_44095</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000633">
        <property type="protein sequence ID" value="KAJ8018101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YAW7"/>
    <dbReference type="OrthoDB" id="8064698at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8018101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="95" mass="10785" checksum="BDC380933074D7CA" modified="2023-09-13" version="1">MSFNVVITGDLKPSRQCAAAAAKANRMLGLIKRNFSSFSKEIVLNLYKQLVRPHLDYAAQAWSPFYDKDKFLLEQVQRIESHLPNTRGETPTLSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YB01</accession>
    <name>A0A9Q0YB01_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">MADS-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_43112</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000210">
        <property type="protein sequence ID" value="KAJ8018730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YB01"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002100">
        <property type="entry name" value="TF_MADSbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036879">
        <property type="entry name" value="TF_MADSbox_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00319">
        <property type="entry name" value="SRF-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55455">
        <property type="entry name" value="SRF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MADS-box" evidence="7">
        <location>
            <begin position="15"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="107"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="113"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="133"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="154"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="211"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8018730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="230" mass="25720" checksum="3CECCD87C58E2538" modified="2023-09-13" version="1">MESAMKSVDNAKSQEDKTRQNKSKSRRVKTLFKKAKELQDATGCQVFVYVKDQYNHVKFGGSSNLSSKFKQGQLSLEAAKKKEINFQVVHPRPTVVRHLKSMWPGTVSDEEASCSTNNNNETFVEEGSSIEGDSVEELTTEEEDTEEEYEDGEDSKQEEKNAQGKEGGIYSALQEEEYEDGEDSKQEGKNAQGKEGGMGEEESNNQKEEGVGDEGLEGWVEETETDMDET</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YCW9</accession>
    <name>A0A9Q0YCW9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_39908</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YCW9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8020338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="83" mass="9533" checksum="89EC941C6BA722CF" modified="2023-09-13" version="1">MEDNEVADIPANCGARDTMMRNILKGGAIGMYRTYITYHVTIRQSGHMVVLSYWGKRNEWDVYHIPFDHQACRPYGSPEVLGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YD77</accession>
    <name>A0A9Q0YD77_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_43955</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000552">
        <property type="protein sequence ID" value="KAJ8018194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YD77"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="10"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="31"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="56"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8018194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="153" mass="16685" checksum="93A099D8E6AC8624" modified="2023-09-13" version="1">MHLCMEVMARESLEGIRGRNTEVDPSGTATSEMSQDSSQESSIDDLAAVPPPLPPSASSLIPTTSQTAETPTTSSLLALQQQVQNLQAQVEALSNQPSSLQRATEIRQLKFQEELLKIEMTKVDLQRQTLDVQKKILAAMEIYGAKAVFSPET</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YDL4</accession>
    <name>A0A9Q0YDL4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phosphatidylinositol 4-kinase beta</fullName>
            <ecNumber evidence="2">2.7.1.67</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_40525</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19877"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
            <dbReference type="ChEBI" id="CHEBI:58178"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.67"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:19878"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Rough endoplasmic reticulum membrane</location>
            <topology evidence="6">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.67" evidence="2"/>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YDL4"/>
    <dbReference type="OrthoDB" id="10264149at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030867">
        <property type="term" value="C:rough endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004430">
        <property type="term" value="F:1-phosphatidylinositol 4-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05168">
        <property type="entry name" value="PI4Kc_III_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1070.11:FF:000004">
        <property type="entry name" value="Phosphatidylinositol 4-kinase, catalytic, beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015433">
        <property type="entry name" value="PI3/4_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001263">
        <property type="entry name" value="PI3K_accessory_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057754">
        <property type="entry name" value="PI4-kinase_beta/PIK1_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049160">
        <property type="entry name" value="PI4KB-PIK1_PIK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048:SF22">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4-KINASE BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21245">
        <property type="entry name" value="PI4KB-PIK1_PIK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00915">
        <property type="entry name" value="PI3_4_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51545">
        <property type="entry name" value="PIK_HELICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PIK helical" evidence="10">
        <location>
            <begin position="64"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="9">
        <location>
            <begin position="596"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="20"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="83"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="273"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="281"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00036767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8020827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="879" mass="99175" checksum="90B0B6EA9C5093B8" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YEQ5</accession>
    <name>A0A9Q0YEQ5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_42587</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000096">
        <property type="protein sequence ID" value="KAJ8019064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YEQ5"/>
    <dbReference type="OrthoDB" id="2286242at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8019064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="110" mass="12574" checksum="EC69A8A969A66AF7" modified="2023-09-13" version="1">MDRLSPPPKLQMEGNLSENWKRFKQQFLLYLTSAGYEGKEAKIKTSLLLHVIGEEALDIYNTFTFTNDAEGNPTNMNLEIVMDKFQNYCTPKKNITTRGINSSLARNAVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YFC8</accession>
    <name>A0A9Q0YFC8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_44888</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010001401">
        <property type="protein sequence ID" value="KAJ8017596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YFC8"/>
    <dbReference type="OrthoDB" id="6128444at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8017596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="309" mass="37071" checksum="0D92E8DE9BFF643F" modified="2023-09-13" version="1">MAEAEIQQPPRKKTRAEYQRDYRRRIKESKEIYYVFREHEKIRSRLWRLNRNSEVKAKEREQARIRAQRYRQKYKGERSSQSAKMSKKVTRAEKQALQEKWRKAKRKYRSNMSSQKRRRVNELRRQKHAESKHVSTIHNQSPDPSSEGLFTSDSARRKAVSRSLRVLPKDPQRFAEVITYMVKTATPKKKNALKRRMILSPSQKKRQDFLNKNSVKAIIQQIKHSRSRKDLELRQLLHIATTIKKKKNNLRKKTSHREFGLSWNFLKKPVQHHLTSNRKKRNDAFNTAKARVVTDFYNRSDITRELPLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YFC9</accession>
    <name>A0A9Q0YFC9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA-directed DNA polymerase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_38905</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YFC9"/>
    <dbReference type="OrthoDB" id="6153129at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33206:SF1">
        <property type="entry name" value="DNA-DIRECTED DNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33206">
        <property type="entry name" value="PROTEIN CBG10425"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8021643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="329" mass="38343" checksum="646E8F260406B14A" modified="2023-09-13" version="1">MQKPRRSLIGSMFGQKILLITPIVKWYLNHGLQVTRIYQVIEYSPESCFRVFGEAVSNARRAGDSDPSKAIIADTMKLIGNSSYGKTITNREKHTNVKLGDASTSSRMINSSTFRDLNFIADDCYEVELAKSTIKMDLPIQIGLFVYQYAKLRMLQFYYDYLCKFFDVNKWEYVEMDTDSAYISTAGNRLEDILKPDMTETFHNEKHFWFPREDTEENKRFDKRTPGLFKVEWQGDGIIALSSTMYYCFGGIKEDKLSCKGVNKKRNHIDKELYENVLATRQTSSCENKGFRVKDNQMYTYSLRKDAFTYFYPKRKVLEDGITTTYLDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YG10</accession>
    <name>A0A9Q0YG10_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">UMP-CMP kinase 2, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_41082</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8019467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YG10"/>
    <dbReference type="OrthoDB" id="425602at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004798">
        <property type="term" value="F:dTMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004550">
        <property type="term" value="F:nucleoside diphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006233">
        <property type="term" value="P:dTDP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006235">
        <property type="term" value="P:dTTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006227">
        <property type="term" value="P:dUDP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10344">
        <property type="entry name" value="THYMIDYLATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10344:SF4">
        <property type="entry name" value="UMP-CMP KINASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8019467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="556" mass="63413" checksum="E94CFCEDAD6DFFFD" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YJX9</accession>
    <name>A0A9Q0YJX9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glucose-repressible alcohol dehydrogenase transcriptional effector</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_36552</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YJX9"/>
    <dbReference type="OrthoDB" id="10253982at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050410">
        <property type="entry name" value="CCR4/nocturin_mRNA_transcr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121">
        <property type="entry name" value="CARBON CATABOLITE REPRESSOR PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121:SF98">
        <property type="entry name" value="ENDONUCLEASE_EXONUCLEASE_PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="2">
        <location>
            <begin position="69"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="304"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="304"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="314"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8023963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="395" mass="45098" checksum="408D8FFB6633A3A9" modified="2023-09-13" version="1">MAESLQREWRSKESVSSEPSSGSGFSVLTYNILAECNLKEWSYPFCPATHRGTYQRHPQLMVELKFHEDTDVICLQEVETEYFHQLLAPSLQQLGFQGHHFPKVGKEEGVAIFYKVSSMALLHHQEVVFKDIFAKEAQQSVGISHRQIVVERCNHESVALAFTLRHRCTGKVVCVLNVHLFWKTKEYLDVNTLQAAWAVRILKETAGQDVATIACGDFNHTPDQPGYDVVAIGNINNKSLDQLQLFKVGKTCLVDLVPSWFHHSHHALHSAYSDIMGREPSFTVYTDEKAEVWQSMHDQFEETSSVETAPSLQDHSEKGKSDPDKENRGENCISCLPMDRRTLDYIWYDSNYLSCQKILDVVDATIIKPFWACPNRVFPSDHMLLKAEFSWNDVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YLC6</accession>
    <name>A0A9Q0YLC6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transcription factor 15</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_34501</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YLC6"/>
    <dbReference type="OrthoDB" id="10055449at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032502">
        <property type="term" value="P:developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11465">
        <property type="entry name" value="bHLH_TS_scleraxis_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000010">
        <property type="entry name" value="Scleraxis bHLH transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050283">
        <property type="entry name" value="E-box_TF_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23349">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, TWIST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23349:SF42">
        <property type="entry name" value="BHLH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="7">
        <location>
            <begin position="77"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="21"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="173"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="38"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="186"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8024562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="198" mass="22312" checksum="C58ABDCF5BF1D788" modified="2023-09-13" version="1">MYGNTTIQAMAKRKFDRAFSMDSGIFPDQQTDEERRSNSSSPGDTFGDTCNNKCFTNQQGLGEDQGELSPGGTHYRGARTTANARERDRTHSVNSAFTALREMIPTEPKDRKLSKIETLRLATSYINHLGTLLLLGDEDIDQPCMHRAMMTPGSENRPRVICTFCLSRVRNKQSRHGKMNVPRGIVGHHHHHHAAMMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YQZ2</accession>
    <name>A0A9Q0YQZ2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_33588</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YQZ2"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8025890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="93" mass="10616" checksum="45FB25F70B581575" modified="2023-09-13" version="1">MTNDAPHCYSSPIQPRKPTQSPSPSMVTRVHQIPRAVPQQSLDSNRRLTFPVLNRIPFQLSLKLFPLSRSPFVEANYPQIVTSRRSCTLTPMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YRI7</accession>
    <name>A0A9Q0YRI7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit beta</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HOLleu_36659</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Nanhai2018</strain>
            <tissue evidence="16">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4-bisphosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18373"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57658"/>
            <dbReference type="ChEBI" id="CHEBI:58178"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.154"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:18374"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3-phosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12709"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
            <dbReference type="ChEBI" id="CHEBI:58088"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.137"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:12710"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the PI3/PI4-kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8024046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="67688at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005942">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016303">
        <property type="term" value="F:1-phosphatidylinositol-3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035005">
        <property type="term" value="F:1-phosphatidylinositol-4-phosphate 3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043491">
        <property type="term" value="P:phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04012">
        <property type="entry name" value="C2A_PI3K_class_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05166">
        <property type="entry name" value="PI3Kc_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06883">
        <property type="entry name" value="PX_PI3K_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1520.10:FF:000006">
        <property type="entry name" value="Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1070.11:FF:000003">
        <property type="entry name" value="Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1010.10:FF:000001">
        <property type="entry name" value="Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.70">
        <property type="entry name" value="Phosphatidylinositol 3-kinase, accessory domain (PIK)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015433">
        <property type="entry name" value="PI3/4_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002420">
        <property type="entry name" value="PI3K-type_C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001263">
        <property type="entry name" value="PI3K_accessory_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042236">
        <property type="entry name" value="PI3K_accessory_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000341">
        <property type="entry name" value="PI3K_Ras-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003903">
        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048:SF14">
        <property type="entry name" value="LD28067P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00792">
        <property type="entry name" value="PI3K_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00794">
        <property type="entry name" value="PI3K_rbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00613">
        <property type="entry name" value="PI3Ka"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00142">
        <property type="entry name" value="PI3K_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00144">
        <property type="entry name" value="PI3K_rbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00145">
        <property type="entry name" value="PI3Ka"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
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    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="534"/>
            <end position="623"/>
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        <location>
            <begin position="771"/>
            <end position="922"/>
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        <location>
            <begin position="938"/>
            <end position="1114"/>
        </location>
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        <location>
            <begin position="1181"/>
            <end position="1459"/>
        </location>
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        <location>
            <begin position="1495"/>
            <end position="1612"/>
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            <begin position="1639"/>
            <end position="1757"/>
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            <begin position="1"/>
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            <begin position="414"/>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51545"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51546"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ8024046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1766" mass="198299" checksum="314671B7D7310F14" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YRP7</accession>
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        <recommendedName>
            <fullName evidence="5">THAP-type domain-containing protein</fullName>
        </recommendedName>
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        <name evidence="6" type="ORF">HOLleu_31296</name>
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    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Echinozoa</taxon>
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            <taxon>Aspidochirotacea</taxon>
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            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
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    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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            <begin position="4"/>
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        </location>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAJ8026471.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="75" mass="8453" checksum="641B0CC398719A7D" modified="2023-09-13" version="1">MPECPAQGCLNQQGTGEAKRKPFFFISDGKYPTKRGLSQRWLHSIGTGHTVHNFTFGRHKVVCVDHFPGIALRRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
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        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
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        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
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    </reference>
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        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
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            <begin position="12"/>
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        </location>
    </feature>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAJ8026741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="174" mass="18956" checksum="01B7F19B1AC84453" modified="2023-09-13" version="1">MPHIERKNPIVFGGGQRSPGVTGGQNLKTLLTQYLEMPHIERKNPIVFGGGQRSPGVTGGQNLKTLLTQYLEMRYGKRKNPIIFGGGERSSGVTGGQTLKTSLTRYLKMRYGKRKNPIIFGGGERSSGVTGGQTLKTLLTRYLKMRYGKRKNPIIFGGGERSSGVTGGQTLKTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1B984</accession>
    <name>A0A9Q1B984_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_44717</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Echinodermata</taxon>
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            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
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            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
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    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010001143">
        <property type="protein sequence ID" value="KAJ8017690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1B984"/>
    <dbReference type="OrthoDB" id="8963689at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="98"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8017690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="204" mass="24332" checksum="18F3374A9BA25867" modified="2023-09-13" version="1">MESIDERMESSREFNFNAAFLELSRNKFVCSPSVEELDSLKKSEIVKVAKHYGIEFHLLMRKDVIKSYVLEYLVDESILPSTVLETAITVPTDNTFELKRLEMQMQKEKEEREMQMQKEKEEREMQMQREEKAREHEFRMKQLELGVIKGSDPKVGLDTGVFDISKHVKFVPKFQEDNVEKFFNRFEKLGEQLKWPRDKWSILI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BAT0</accession>
    <name>A0A9Q1BAT0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_41545</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8019805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BAT0"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8019805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="80" mass="8987" checksum="68388357E1F64F40" modified="2023-09-13" version="1">MESSREFNFNAAVLELSRSENCLRVQRLLSSIIHRLALSLFARGTSSYATARYSTLQVYYRTIVNVHALPALGPDQANIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BBK0</accession>
    <name>A0A9Q1BBK0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_39137</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BBK0"/>
    <dbReference type="OrthoDB" id="6022714at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031380">
        <property type="entry name" value="SIX6OS1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15676">
        <property type="entry name" value="S6OS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="249"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="402"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="473"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="519"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="19"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="162"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="249"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="321"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="402"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="428"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="538"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="557"/>
            <end position="573"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8021831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="573" mass="63312" checksum="12456B4F50FF5021" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BBM4</accession>
    <name>A0A9Q1BBM4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_40346</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BBM4"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="375"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="192"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="114"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="131"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="221"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="240"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8020690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="415" mass="46434" checksum="96D58DF38358FE32" modified="2023-09-13" version="1">MEDHDYAELGAPKGSNSKKSKQGKQVKQDKTPNGTAALKPPTRDKRKGDVRRTSSTYGLKDSEKVHRNGKKKDSPESLEIAPYATIPRKSDPSKKQNKAPSPNGSVKHTNGDVQSRDKSPHTDVHNMNGSARKDSKEMPEKDFSGLYAKVSKRGNGDMKSTETKVTLKEQKETTVNQQGDDLSQLYAEVKKSGRKQLPQQEVISHDKDDTTLKTNDIHNSASKDGEKPADEVFESNPYTDIKRRTSYHGKENIGSPPRLAKRGSLSLSFRSFRSQKSDVTDEGNESLTSNSLYNLIPEEPDTPMPDIELFEEAPPLPPENAVLQGQDNSAFQPENANNIYSTEIDVENFEDEGYKEAMRRRDMVKKKQETQKRRVRFLVVTTVLLFVVLLIVIGVVVFLLMEGGYFDCILGKKSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BBS6</accession>
    <name>A0A9Q1BBS6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Kappa-type opioid receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_41342</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8019668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BBS6"/>
    <dbReference type="OrthoDB" id="10042731at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00637">
        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24243">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24243:SF224">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 19-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="36"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="80"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="118"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="161"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="221"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="265"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="10">
        <location>
            <begin position="16"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8019668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="345" mass="39591" checksum="5712A862EE2276D1" modified="2023-09-13" version="1">MASCIVTLSSVLAIVGNSLNIIVLTTRKFLTKPHAYFLISLAVADLLTGMVDSFSVYSSITYTWPYGEPFCLLTNYVREALLEISLLMILCLNLERYIAISYPFRSKIWLTKNRSKAFIFLCWFVTFAFKIYLFFDVVYIVEYDKDIYACTPEYDNYKGFAIFSSVAIEVPALIILTITSVKLIVALKRLNSRKHREMFHGSLQRRYSTNSRNLRKNLHGFKVLVVIATVSYISLLPSLSLRLLYISDNDEEESAALNLSWALEFSFFWLASCGSFANVFVYYFMDRNFSRCLRDMLQELKRALKRKRTTVLSRGCPLKENGSPTNQPLSVISDNRLNSQACTNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BDW0</accession>
    <name>A0A9Q1BDW0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">IgGFc-binding protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_39077</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BDW0"/>
    <dbReference type="OrthoDB" id="10005154at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035234">
        <property type="entry name" value="IgGFc-bd_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46534:SF1">
        <property type="entry name" value="IGGFC-BINDING PROTEIN N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46534">
        <property type="entry name" value="IGGFC_BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17517">
        <property type="entry name" value="IgGFc_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IgGFc-binding protein N-terminal" evidence="1">
        <location>
            <begin position="123"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF17517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8021783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="403" mass="44704" checksum="3CA3D93990437C9E" modified="2023-09-13" version="1">MIFYHFNTASKEITEKFIFTFLAVKCTDPTTEIFIGTKFSDIAASGNITFPLIDIPNITFSLNPGRGETFEVPLTVVPSESELNQKVETSVVIASDEEVLVYGHQRCHGSNNNRATAFRVRGIEDLGTEYWVITHQSKGKSQVGVVATKDHTNISFNGVYINTALNLNKYEIFYFGGSYDLTGTYIAADKPIFVIAGNSADISPYPNTIYIDSFNKCLPPVKDWKKHFTLVPFPPESQSFTAKILSSHDNCTLEYVNGTGSHDSVSLSKGEVKVLNSLTVLNISSSEGILVAQFSKGSRGCGRYSNPSMFLTPPYGHAERNEFIFPVFLFEKSYITIWLPPASNILLDNKTVSWNEIGEGTNGYNIVQITLPSRGFHVIHSSKNITAVVCGVGHYKYYSYSLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BEE7</accession>
    <name>A0A9Q1BEE7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_36064</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BEE7"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8023585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="82" mass="9319" checksum="7D79C4A156317353" modified="2023-09-13" version="1">MIRKWIENLFQPVHNIEGLYSSHGRFLLEIKHTTNCNRVTAAAASNTASRLQVNLAFISKGSVTSLAWITDHCDIIVRRRPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BEX7</accession>
    <name>A0A9Q1BEX7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Heparan sulfate 2-O-sulfotransferase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_35407</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sulfotransferase 3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8025251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BEX7"/>
    <dbReference type="OrthoDB" id="10019582at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007734">
        <property type="entry name" value="Heparan_SO4_2-O-STrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005331">
        <property type="entry name" value="Sulfotransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12129">
        <property type="entry name" value="HEPARAN SULFATE 2-O-SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12129:SF15">
        <property type="entry name" value="URONYL 2-SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03567">
        <property type="entry name" value="Sulfotransfer_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8025251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="331" mass="38558" checksum="4A4D078C8AC4EF8E" modified="2023-09-13" version="1">MATTLKKRGLPYKVTFLLFLWMAVMLMYVCLIMEPSNIANDGEGKLKYLPRRLSGPPQKETSTSSLPLHFELKPINGTNVTILHNAVPKTGSRTLELFLRLLGTRRMSLLHCYTFPPNISKNADQIRDFVGSMKQGSYTRGHFPFTNVSRWYSNTRYINIVRPPIQRLLSNYYYDLYGDKQQNQSPSFHEPTLDECLEKEVCFIKVYSYYKQTTLRTFCGQDPRCNQYTRWTLEKAIENARKYLFIGVTNQLNESISILETLLPDLFNGLTKLFLAGSGQVTSDFKTVKKMQLQNKSLEFLQRDMDLEFEFYQFVLNGFNRALTELMLKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BGF3</accession>
    <name>A0A9Q1BGF3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">B box-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_36964</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8024274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BGF3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="B box-type" evidence="3">
        <location>
            <begin position="125"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="183"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8024274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="608" mass="68418" checksum="27531464E5FE5701" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BHJ7</accession>
    <name>A0A9Q1BHJ7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Sodium/bile acid cotransporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_34031</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bile acid:sodium symporter (BASS) (TC 2.A.28) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8026238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BHJ7"/>
    <dbReference type="OrthoDB" id="203097at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002657">
        <property type="entry name" value="BilAc:Na_symport/Acr3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004710">
        <property type="entry name" value="Bilac:Na_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10361:SF65">
        <property type="entry name" value="ILEAL SODIUM_BILE ACID COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10361">
        <property type="entry name" value="SODIUM-BILE ACID COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01758">
        <property type="entry name" value="SBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0769">Symport</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040452667" evidence="9">
        <location>
            <begin position="24"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="159"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="202"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="229"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="257"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="300"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="331"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="361"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="392"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="425"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="467"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="477"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="500"/>
            <end position="513"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8026238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="524" mass="57362" checksum="E13C41B5285F3B27" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BJG5</accession>
    <name>A0A9Q1BJG5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_29805</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8027758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BJG5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="4">
        <location>
            <begin position="17"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="247"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="156"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="180"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8027758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="327" mass="36741" checksum="A590B90F2A547826" modified="2023-09-13" version="1">MFQMSRTGKTCVEDDVCTTCDKSGHTFRNCPVSFSSKFKQSTAWVAGEASPVKIDSSDTNLLDMEGVEMSEQGSQLDVENSDGVELESISQPEVIPDTQDPSVMDTASEVNSQEDLFEGEAAAELLAAAPKNWAEVTEDGQMKSVNVIKEKEKTRINEKDSLVPEKAQSQPSTLKGAGTLVQSRVISSQPVADQDPSKSSKRKSVSPPTPTKLVGRTRSRSSTRFLTEDPKDIVKMTQIFIEEEPWHSCYAKDCKEVFSKYTLLKEHAQFFHPDLKPRSYSCLLKSCKADYTTPQDWIWHVACEHPDFVEKHEVEFFDRYFLKRNSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BLQ9</accession>
    <name>A0A9Q1BLQ9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase</fullName>
            <ecNumber evidence="7">3.5.1.26</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Aspartylglucosaminidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Glycosylasparaginase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">N4-(N-acetyl-beta-glucosaminyl)-L-asparagine amidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HOLleu_31110</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Nanhai2018</strain>
            <tissue evidence="16">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Cleaves the GlcNAc-Asn bond which joins oligosaccharides to the peptide of asparagine-linked glycoproteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>N(4)-(beta-N-acetyl-D-glucosaminyl)-L-asparagine + H2O = N-acetyl-beta-D-glucosaminylamine + L-aspartate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11544"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15947"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:58080"/>
            <dbReference type="EC" id="3.5.1.26"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Ntn-hydrolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.26" evidence="7"/>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BLQ9"/>
    <dbReference type="OrthoDB" id="188713at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003948">
        <property type="term" value="F:N4-(beta-N-acetylglucosaminyl)-L-asparaginase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04513">
        <property type="entry name" value="Glycosylasparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.30:FF:000003">
        <property type="entry name" value="N(4)-(Beta-N-acetylglucosaminyl)-L-asparaginase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.30">
        <property type="entry name" value="(Glycosyl)asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000246">
        <property type="entry name" value="Peptidase_T2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10188">
        <property type="entry name" value="L-ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10188:SF6">
        <property type="entry name" value="N(4)-(BETA-N-ACETYLGLUCOSAMINYL)-L-ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01112">
        <property type="entry name" value="Asparaginase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040193642" description="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase" evidence="15">
        <location>
            <begin position="24"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="166"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="11">
        <location>
            <position position="208"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="236"/>
            <end position="239"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="259"/>
            <end position="262"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Cleavage; by autolysis" evidence="13">
        <location>
            <begin position="207"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00050421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00053295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00066729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00078726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00079301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00080645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR600246-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR600246-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR600246-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ8028775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="344" mass="37043" checksum="B0FFAD8DC4FA757D" modified="2023-09-13" version="1">MNIKSVYSILYSVTVLIVPPALCSDVIPLIINTWNFSAATSPTWQGLVEGVSALDTVVQGCSNCEGLQCDGSVGYGGHPDDVGETTLDAYVMDGKTLDIGAVGDLRRVKNAIHVARAVMDFTKHTLLVGESAKEFAVSMGFKEESLSTNKSREDYLEWQKNKCQPNFRQNVVPDPTKSCGPYKPSPGLRSEHMPRKASQDISQENHDTIGMVAIDLSINVAAGVSTNGLNHKIHGRVGDSPIPGAGGYADNEVGGAAATGDGDVMMRFLPSFFVVEQMRNGVHPEDACEKAIRRIMKYYPDFSGAVVAANINGTYGAACHNAYFVYNIQNPSMPKMKTVNVTCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BPK8</accession>
    <name>A0A9Q1BPK8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_26795</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BPK8"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="123"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8030383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="151" mass="16344" checksum="F9C986A7DF6484C4" modified="2023-09-13" version="1">MSLVRSGVRSFRAFLFNETQAQPNEKLVDNVITILAITGSTLLGGLVGNGHIVTYVPYFNIELEYSGWKSFLLSAVAAVATIIGMKIPLVSTWTPLRFLVTVFIGISLGTNVGMVIGQLQMEIVVFIASGVSLVTTTAVLLLQEIAMYVFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BQ27</accession>
    <name>A0A9Q1BQ27_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">mannose-6-phosphate isomerase</fullName>
            <ecNumber evidence="4">5.3.1.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Phosphohexomutase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Phosphomannose isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HOLleu_27183</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Nanhai2018</strain>
            <tissue evidence="16">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-mannose 6-phosphate = D-fructose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:12356"/>
            <dbReference type="ChEBI" id="CHEBI:58735"/>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
            <dbReference type="EC" id="5.3.1.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; alpha-D-mannose 1-phosphate from D-fructose 6-phosphate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the mannose-6-phosphate isomerase type 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.8" evidence="4"/>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BQ27"/>
    <dbReference type="OrthoDB" id="6605218at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004476">
        <property type="term" value="F:mannose-6-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009298">
        <property type="term" value="P:GDP-mannose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07011">
        <property type="entry name" value="cupin_PMI_type_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.441.10:FF:000001">
        <property type="entry name" value="Mannose-6-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000044">
        <property type="entry name" value="Mannose-6-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.441.10">
        <property type="entry name" value="Phosphomannose Isomerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001250">
        <property type="entry name" value="Man6P_Isoase-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016305">
        <property type="entry name" value="Mannose-6-P_Isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018050">
        <property type="entry name" value="Pmannose_isomerase-type1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046456">
        <property type="entry name" value="PMI_typeI_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046457">
        <property type="entry name" value="PMI_typeI_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046458">
        <property type="entry name" value="PMI_typeI_hel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00218">
        <property type="entry name" value="manA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10309">
        <property type="entry name" value="MANNOSE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10309:SF0">
        <property type="entry name" value="MANNOSE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01238">
        <property type="entry name" value="PMI_typeI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20511">
        <property type="entry name" value="PMI_typeI_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20512">
        <property type="entry name" value="PMI_typeI_hel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001480">
        <property type="entry name" value="Mannose-6-phosphate_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00714">
        <property type="entry name" value="MAN6PISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00965">
        <property type="entry name" value="PMI_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 16" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Phosphomannose isomerase type I catalytic" evidence="14">
        <location>
            <begin position="6"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphomannose isomerase type I helical insertion" evidence="15">
        <location>
            <begin position="187"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphomannose isomerase type I C-terminal" evidence="13">
        <location>
            <begin position="341"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="299"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="280"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001480-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR001480-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF20511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF20512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ8030700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="427" mass="47365" checksum="775F3C9C93599108" modified="2023-09-13" version="1">MSVPKVFTLKCVVMNNAWGRVGTESEVALLRQGGDETFELQHDMTYAELWMGTHPRGPSILSSSDFAGTSLTDWIKDHPECLGKKVRAKFNDKLPFLFKILSINKTLSLQAHPNKSHAEQLNATQPDKYVDDNHKPEMALALTPFEGMNGFKPVSEIVRLLQGIPEFKAAVGEEHCKDLFAVCKTDPKGSSDAAKAALKKCFTAFMNRDEDLVKEEIRKLEKRFQKLADLGQDITDYDGDIFLRLCREYPLDTGCFASYFFNVLVLKPGEAMFLGPNEPHAYMSGNCVECMSCSDNVVRAGLTPKYKDVPTLCEMLTYITNTPEERIFEVAMDPTDPAVTIYDPPIPDFAIAKIELPPETKEYTIAAYDSGSIIIVIRGAASASSPSIPQPLSLVRGSIFFVAADEPVHLKLDSNEGLLAFRAYCPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BRH8</accession>
    <name>A0A9Q1BRH8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_25044</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BRH8"/>
    <dbReference type="OrthoDB" id="6076093at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031746">
        <property type="entry name" value="DUF4732"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37153">
        <property type="entry name" value="CHROMOSOME 19 C19ORF81 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37153:SF1">
        <property type="entry name" value="HYPOTHETICAL LOC292874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15876">
        <property type="entry name" value="DUF4732"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="67"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8031743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="238" mass="27045" checksum="A0C5964F3996BC81" modified="2023-09-13" version="1">MPISSSRQRLMPAAFEKPSAQSQPNLSIPKSHESIKKHARNAQRDKLTLPRINQLQIDDLVERQPHSKYPSFSSPFYKPSSPRHGGRSGTPSKKQAQTDPRREIAFKKIKVFSGPPELEPLTLVFSHSDESLSHQDVLHSIRKVGNVSTEDIRNIQFIHMNVVLGSAGVPSRWLIRVKGYEASQELLRAGLVIKGERINLRRCDDVNSEEYRAFVKRKTLLESDQHSEIQRLLVDNLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BRJ2</accession>
    <name>A0A9Q1BRJ2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_24563</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BRJ2"/>
    <dbReference type="OrthoDB" id="6280085at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8031388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="260" mass="28946" checksum="A27E7D6DF5BB237C" modified="2023-09-13" version="1">MDGYGRGDSRAGSRGAPRPDCVPALDLGHLADKNNGLRNPVTDYEPKLSPPDTASTVSWGYHDYSTRTSQRGSDSRRPPFALHHAPEPSPHVTGRSQRPEGVPKLNLDGSDDTRPVSAKHQQASRRGPLIPPYHTHENQQSEHVEHGTRVKSGRGQRSPTGVDLERKSEAEKLAESRKREHIIHTVLTDQLSRAAISDPQQNIDDDPASQRPLFGVEQKPKIRNLHYTKVKDTTTGYRAATCKLYQLNARILTRDGHFRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BT04</accession>
    <name>A0A9Q1BT04_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_25699</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BT04"/>
    <dbReference type="OrthoDB" id="10044919at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24228">
        <property type="entry name" value="B2 BRADYKININ RECEPTOR/ANGIOTENSIN II RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24228:SF72">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0807">Transducer</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8032230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="150" mass="16723" checksum="5CA0E1D688D40AA6" modified="2023-09-13" version="1">MVPKGLNEQSKGLFKPCIGQGRPERNGRVMNASPVSENQVNITKNLFYLFVTFLMCFGPAVVSEAFSANVIVLSYTRAIIAFNSCLNPAIYGIKHPLFRQVMYNILFRKWSEIPEPAFKWMNSEVPCPAGSRSKDLDAISITVSEKSTVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BTV5</accession>
    <name>A0A9Q1BTV5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_26352</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BTV5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8032726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="79" mass="8927" checksum="103C0091D067D397" modified="2023-09-13" version="1">MIRTVTRRQKTRVVNACMKDPSRVNRTLKRQYASRAGECAAKRTKGVRRKKFTDRLGEQSGEQPGPSTVTADLPYDSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BTW4</accession>
    <name>A0A9Q1BTW4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">T-complex protein 11-like protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_26039</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TCP11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BTW4"/>
    <dbReference type="OrthoDB" id="276323at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008862">
        <property type="entry name" value="Tcp11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12832:SF11">
        <property type="entry name" value="LD23868P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12832">
        <property type="entry name" value="TESTIS-SPECIFIC PROTEIN PBS13 T-COMPLEX 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05794">
        <property type="entry name" value="Tcp11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="286"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="49"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="306"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8032496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="549" mass="61767" checksum="37EE85D614A2AF40" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BU77</accession>
    <name>A0A9Q1BU77_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein ALP1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_23039</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8032942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BU77"/>
    <dbReference type="OrthoDB" id="1681765at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027806">
        <property type="entry name" value="HARBI1_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13359">
        <property type="entry name" value="DDE_Tnp_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE Tnp4" evidence="3">
        <location>
            <begin position="2"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8032942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="210" mass="23529" checksum="F831DBB89D2A8C1F" modified="2023-09-13" version="1">MAVVGADYEFIMVDAGVNGRVSDGGVIAVTEFGRLLDDGSLGLPEPAPLHQNNVTAMPYVFVGDDAFAVSENLLKPYGGDRLESDQRIYNYRLSRARRVTENAFGILTARFGVFQKAMQLSPEKATLITMICCYLHNFRKKSRCYIGPGALDWEDANHEMHAGEWRASQRQLEGLRATMNRNAGNTARLVRDAFRHYFCTQGQVPWQDSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BUB6</accession>
    <name>A0A9Q1BUB6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">UDP-glucuronosyltransferase 2C1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_22869</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8032813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BUB6"/>
    <dbReference type="OrthoDB" id="5983272at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008194">
        <property type="term" value="F:UDP-glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000021">
        <property type="entry name" value="UDP-glucuronosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050271">
        <property type="entry name" value="UDP-glycosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043">
        <property type="entry name" value="EG:EG0003.4 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043:SF145">
        <property type="entry name" value="FI06409P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040151818" evidence="5">
        <location>
            <begin position="22"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="492"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8032813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="520" mass="59225" checksum="35D63C8EBD863EE6" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BUB8</accession>
    <name>A0A9Q1BUB8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_23011</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8032920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BUB8"/>
    <dbReference type="OrthoDB" id="426210at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8032920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="100" mass="11836" checksum="5009D257780037B1" modified="2023-09-13" version="1">MGLIRRTHTYLDDESFKCLFQALVKQHLEYPASVWNPFPHLKRDIHLLENVQRRSATRIPSLKGLSYQERLHKLNLPILRYRGLSGGMSEVFKMYSSRHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BUE6</accession>
    <name>A0A9Q1BUE6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Regucalcin</fullName>
            <ecNumber evidence="7">3.1.1.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Gluconolactonase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HOLleu_22891</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Nanhai2018</strain>
            <tissue evidence="17">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-glucono-1,5-lactone + H2O = D-gluconate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10440"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16217"/>
            <dbReference type="ChEBI" id="CHEBI:18391"/>
            <dbReference type="EC" id="3.1.1.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="15">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the SMP-30/CGR1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.17" evidence="7"/>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8032829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BUE6"/>
    <dbReference type="OrthoDB" id="423498at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004341">
        <property type="term" value="F:gluconolactonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019853">
        <property type="term" value="P:L-ascorbic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008367">
        <property type="entry name" value="Regucalcin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013658">
        <property type="entry name" value="SGL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005511">
        <property type="entry name" value="SMP-30"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10907">
        <property type="entry name" value="REGUCALCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10907:SF47">
        <property type="entry name" value="REGUCALCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08450">
        <property type="entry name" value="SGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01791">
        <property type="entry name" value="REGUCALCIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01790">
        <property type="entry name" value="SMP30FAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63829">
        <property type="entry name" value="Calcium-dependent phosphotriesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="SMP-30/Gluconolactonase/LRE-like region" evidence="16">
        <location>
            <begin position="15"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="14">
        <location>
            <position position="238"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="238"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00008853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00016808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00032464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR605511-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR605511-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF08450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ8032829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="333" mass="37545" checksum="82DD969382E0BD9B" modified="2023-09-13" version="1">MSITVVCKKAGYLLEGPHWDDTNNLLYFVDILGKAINKWDPVRDTIEKYDVPDQSIGAAVLTDRNNMLLALTRRIAFLDWRTKKLFDIQEVEDETNTKNRFNDGKCDPAGRFWAGKVCFNLSTNPSMDTQTSLVMLHRIIPVLFKLGVAASTMGPEEELGGVLREQGQLYCYHKDRSLTVHADKISISNGLAWSSCRKFMYYIDSMKYTVDVFDFDLESGQTSNRRVAIDMKSEGIPDGMCIDAEDMLWVAVFNGGKVIRYNPKTGEKLSELKFPTLNTTSCCWGGPNYDELYVTSAEHFLTEQQLKEQPTGGSVFRVTGLGVKGTKSDIYNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BUF9</accession>
    <name>A0A9Q1BUF9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Beta-1,4-glucuronyltransferase 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">I-beta-1,3-N-acetylglucosaminyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Poly-N-acetyllactosamine extension enzyme</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">HOLleu_23078</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="22">Nanhai2018</strain>
            <tissue evidence="22">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>3-O-[beta-D-Xyl-(1-&gt;4)-Rib-ol-P-Rib-ol-P-3-beta-D-GalNAc-(1-&gt;3)-beta-D-GlcNAc-(1-&gt;4)-(O-6-P-alpha-D-Man)]-Thr-[protein] + UDP-alpha-D-glucuronate = 3-O-[beta-D-GlcA-(1-&gt;3)-beta-D-Xyl-(1-&gt;4)-Rib-ol-P-Rib-ol-P-3-beta-D-GalNAc-(1-&gt;3)-beta-D-GlcNAc-(1-&gt;4)-(O-6-P-alpha-D-Man)]-Thr-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46860"/>
            <dbReference type="Rhea" id="RHEA-COMP:15023"/>
            <dbReference type="Rhea" id="RHEA-COMP:17482"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:142405"/>
            <dbReference type="ChEBI" id="CHEBI:177336"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyltransferase 49 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8032977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BUF9"/>
    <dbReference type="OrthoDB" id="9974378at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015020">
        <property type="term" value="F:glucuronosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035269">
        <property type="term" value="P:protein O-linked glycosylation via mannose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043189">
        <property type="entry name" value="B4GAT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46420">
        <property type="entry name" value="BETA-1,4-GLUCURONYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46420:SF1">
        <property type="entry name" value="BETA-1,4-GLUCURONYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13896">
        <property type="entry name" value="Glyco_transf_49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="12" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="15" id="KW-0464">Manganese</keyword>
    <keyword evidence="13 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00030723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00032175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00032181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00033291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KAJ8032977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="305" mass="34623" checksum="7008DE34AF27F099" modified="2023-09-13" version="1">MANEVTKITSSACFLLFFNISVGLLVTMQALHYFRNSGSLDNAWQIVPSKTPDNMELNTSLDVEKRGDYYIHHVPPSFERTLPILNSVTYVTHCSPHNLRYLPSLLERWNGPVSLAIITSSGDFQAAIRAINSLYVCYPAIALHVTFHVVIPEDHESSISIGNDQRSFEVPCNQLIKYLFENLKGRNFELKGLPYPGNILTNVARLHATTNFILVADIDLSPSLGFYEQTVKFFKEKVTHEDLKKTAFIVPTFEIEEINYVPMDKDDLAIAVDGGHARQFHKETCPHCHKISNYERWFKLKASTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BUQ6</accession>
    <name>A0A9Q1BUQ6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_23105</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8032994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BUQ6"/>
    <dbReference type="OrthoDB" id="6361178at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8032994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="117" mass="12929" checksum="533CC2ED80A84AB6" modified="2023-09-13" version="1">MPHIERKNPIVFGGGQRSPGVTRGQNLKTLLTQYLEMRYGKRKNPIIFGGGQRSPGVTRGQNLKTLLTQYLEMRYGKRKNPIIFGGGQRSSGVTGGQTLKTSLTRYLKKGTSDSSQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BVJ2</accession>
    <name>A0A9Q1BVJ2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_23660</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BVJ2"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040480110" evidence="1">
        <location>
            <begin position="30"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8033425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="120" mass="13818" checksum="87ECF601E91F804C" modified="2023-09-13" version="1">MAVSLPRIYVKHCVGQILIIYFALRAAGAANLDEVSTRQKRDTEGSWYFCFQRPHYPRDCQEVNSYCSMTEVLMALLQMPQTYPAQVMFILLNLMVIQNRLRSIVITVLMEGDGRYFSGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BVV7</accession>
    <name>A0A9Q1BVV7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NACHT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_24174</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BVV7"/>
    <dbReference type="OrthoDB" id="10034282at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312">
        <property type="entry name" value="NACHT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312:SF2">
        <property type="entry name" value="NUCLEOTIDE-BINDING OLIGOMERIZATION DOMAIN-CONTAINING PROTEIN 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NACHT" evidence="1">
        <location>
            <begin position="109"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8033812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="661" mass="76142" checksum="66F06FD1B4758379" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BWN1</accession>
    <name>A0A9Q1BWN1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_20960</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BWN1"/>
    <dbReference type="OrthoDB" id="6417614at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="88"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8034217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="110" mass="12264" checksum="7BF3E97226C6FB3B" modified="2023-09-13" version="1">MQRWRKHGPYLQVRANGSPKQRQSIISGASKDLIHCLCEGAVNTLKGNVQVNRLQKKNCRKHRNTLRTLANKRVSTPSKRKLLLQRGGSFLAALLPPLIGALGSALFARR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BZ98</accession>
    <name>A0A9Q1BZ98_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_22463</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BZ98"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8035289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="105" mass="11752" checksum="7EF75A92723DF144" modified="2023-09-13" version="1">MKEVCTASSARLESGKKQKISVKVTTVKPTSSTSNHTRKMSSYRICLFRINHHREVLMTHGWVLPIVIRKEFGSGSMAVSWIMKTGRPPTANLTVQQILTKIVPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BZG5</accession>
    <name>A0A9Q1BZG5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_22460</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BZG5"/>
    <dbReference type="OrthoDB" id="775972at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF10">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8035286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="65" mass="7099" checksum="3950C3EB061056CE" modified="2023-09-13" version="1">MKSSVAYLGQIKVDGDGLPTTQNKIDDLLNAPNPSYISELQSYLGLLNYYGKFLPNLSALLHPTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C101</accession>
    <name>A0A9Q1C101_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Coadhesin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_17889</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10062639at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00110">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000001">
        <property type="entry name" value="semaphorin-5A isoform X1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000007">
        <property type="entry name" value="Thrombospondin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000002">
        <property type="entry name" value="Unc-5 netrin receptor C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052065">
        <property type="entry name" value="Compl_asym_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001791">
        <property type="entry name" value="Laminin_G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22906:SF46">
        <property type="entry name" value="HEMICENTIN-1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22906">
        <property type="entry name" value="PROPERDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02210">
        <property type="entry name" value="Laminin_G_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00090">
        <property type="entry name" value="TSP_1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01705">
        <property type="entry name" value="TSP1REPEAT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040469870" evidence="5">
        <location>
            <begin position="23"/>
            <end position="1931"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="995"/>
            <end position="1109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1423"/>
            <end position="1445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1516"/>
            <end position="1535"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1597"/>
            <end position="1716"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1758"/>
            <end position="1788"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1858"/>
            <end position="1882"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1911"/>
            <end position="1931"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="1265"/>
            <end position="1320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1516"/>
            <end position="1533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="1636"/>
            <end position="1657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1658"/>
            <end position="1672"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1682"/>
            <end position="1710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1871"/>
            <end position="1882"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8037143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1931" mass="217004" checksum="94B525068C3A7B90" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C160</accession>
    <name>A0A9Q1C160_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DOMON domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_18019</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C160"/>
    <dbReference type="OrthoDB" id="6372137at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="CDD" id="cd09631">
        <property type="entry name" value="DOMON_DOH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045266">
        <property type="entry name" value="DOH_DOMON"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005018">
        <property type="entry name" value="DOMON_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03351">
        <property type="entry name" value="DOMON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50836">
        <property type="entry name" value="DOMON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DOMON" evidence="2">
        <location>
            <begin position="14"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8037248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="91" mass="9726" checksum="22839B9E5BA6BB56" modified="2023-09-13" version="1">MIPEDCNPDAADSCEAFSSWSLQEDNSLLINLQCQTSTYIALGFSKSGGMANSDVYACTSENEVVRSANGNGRDNSPLGLVSKMERGKTLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C1X2</accession>
    <name>A0A9Q1C1X2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="21">Polycystic kidney disease protein 1-like 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">HOLleu_17833</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">Nanhai2018</strain>
            <tissue evidence="21">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the polycystin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="444119at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050982">
        <property type="term" value="P:detection of mechanical stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00146">
        <property type="entry name" value="PKD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd01752">
        <property type="entry name" value="PLAT_polycystin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.60.20:FF:000022">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.220.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.60.20">
        <property type="entry name" value="PLAT/LH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046338">
        <property type="entry name" value="GAIN_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000203">
        <property type="entry name" value="GPS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022409">
        <property type="entry name" value="PKD/Chitinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002859">
        <property type="entry name" value="PKD/REJ-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013122">
        <property type="entry name" value="PKD1_2_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003915">
        <property type="entry name" value="PKD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000601">
        <property type="entry name" value="PKD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035986">
        <property type="entry name" value="PKD_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001024">
        <property type="entry name" value="PLAT/LH2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036392">
        <property type="entry name" value="PLAT/LH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042060">
        <property type="entry name" value="PLAT_polycystin1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051223">
        <property type="entry name" value="Polycystin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046791">
        <property type="entry name" value="Polycystin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014010">
        <property type="entry name" value="REJ_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10877:SF194">
        <property type="entry name" value="LOCATION OF VULVA DEFECTIVE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10877">
        <property type="entry name" value="POLYCYSTIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01825">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00801">
        <property type="entry name" value="PKD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08016">
        <property type="entry name" value="PKD_channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01477">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20519">
        <property type="entry name" value="Polycystin_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02010">
        <property type="entry name" value="REJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01433">
        <property type="entry name" value="POLYCYSTIN2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00303">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00308">
        <property type="entry name" value="LH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00089">
        <property type="entry name" value="PKD"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49723">
        <property type="entry name" value="Lipase/lipooxygenase domain (PLAT/LH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49299">
        <property type="entry name" value="PKD domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50093">
        <property type="entry name" value="PKD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50095">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51111">
        <property type="entry name" value="REJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0969">Cilium</keyword>
    <keyword evidence="14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 17" id="KW-0732">Signal</keyword>
    <keyword evidence="5 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040426403" evidence="17">
        <location>
            <begin position="28"/>
            <end position="4186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="3066"/>
            <end position="3085"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="3273"/>
            <end position="3293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="3313"/>
            <end position="3334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="3463"/>
            <end position="3483"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="3503"/>
            <end position="3529"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="3602"/>
            <end position="3624"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="3871"/>
            <end position="3891"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="3911"/>
            <end position="3931"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="3951"/>
            <end position="3974"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="3986"/>
            <end position="4014"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="4063"/>
            <end position="4082"/>
        </location>
    </feature>
    <feature type="domain" description="PKD" evidence="18">
        <location>
            <begin position="468"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="domain" description="PKD" evidence="18">
        <location>
            <begin position="844"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="domain" description="PKD" evidence="18">
        <location>
            <begin position="1368"/>
            <end position="1417"/>
        </location>
    </feature>
    <feature type="domain" description="REJ" evidence="20">
        <location>
            <begin position="2091"/>
            <end position="2801"/>
        </location>
    </feature>
    <feature type="domain" description="PLAT" evidence="19">
        <location>
            <begin position="3110"/>
            <end position="3227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1802"/>
            <end position="1831"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="3360"/>
            <end position="3381"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="2570"/>
            <end position="2601"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="4082"/>
            <end position="4109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="1816"/>
            <end position="1831"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="3368"/>
            <end position="3381"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="3714"/>
            <end position="3727"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR603915-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAJ8037097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="4186" mass="474947" checksum="4321FF10FE4AF9D1" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C2Y0</accession>
    <name>A0A9Q1C2Y0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_17947</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C2Y0"/>
    <dbReference type="OrthoDB" id="10043504at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="421"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8037194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="456" mass="53110" checksum="B99E0EB26A5D14CC" modified="2023-09-13" version="1">MAFQWKNVPCHFTWGMDRAAKSVHERNSVFQTENAKLYSDDEDACYMFVYLAYLEFVTCQNVNKGTEESLDKVDKVIDRMEDESGKRAFQLVSLANRAWILSLSGEPQEAVAPIISHLLSRYEEYRSSKNIEKETAYLDAIEAFALRRILLEDRAEEKYKSAIRVIPDIAKWHFELGRVVGRSLMTAKAKHEAKIPHFKRALQLKPDYVQCMAKLINSLMRAGHLKDAWVEMARLEKFDISNNPSSICVRASLYIRDGKRDRALEILHEAERLQHSDVYSLIATTYKLEAKSLSPLKREKYLQKSLTYVEKCLCKHPGSFEGITTKFEVLCRLKESADKVKSFLRKLNEERPLPPSFLFPAFQQMITIFEEVNKSNLHPDLEILLSLYESMVTMLEVTLRECLESDSDEKRVCTKKYRPTLQKAQTVVDKYLKENREHEDMNERFREANERLAHVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C364</accession>
    <name>A0A9Q1C364_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_15780</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C364"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8038373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="75" mass="8505" checksum="D77EDB937F43DA67" modified="2023-09-13" version="1">MIFDDICASRRVYITASPSVSEQFTIRTPCIEFLIGEREVNWLYLGTAGLRHSGHPANLVSLSLDCNKYEDNYCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C8L5</accession>
    <name>A0A9Q1C8L5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Mediator of RNA polymerase II transcription subunit 26</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_14133</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8039966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C8L5"/>
    <dbReference type="OrthoDB" id="10057627at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070847">
        <property type="term" value="C:core mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010628">
        <property type="term" value="P:positive regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.10">
        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042376">
        <property type="entry name" value="MED26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031416">
        <property type="entry name" value="Med26_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035441">
        <property type="entry name" value="TFIIS/LEDGF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017923">
        <property type="entry name" value="TFIIS_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15201">
        <property type="entry name" value="CRSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15201:SF1">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08711">
        <property type="entry name" value="Med26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15693">
        <property type="entry name" value="Med26_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47676">
        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51319">
        <property type="entry name" value="TFIIS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TFIIS N-terminal" evidence="4">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="61"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="319"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="4"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="75"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="139"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="191"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="228"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="244"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="319"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="337"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8039966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="430" mass="47351" checksum="C39D0C8A71079854" modified="2023-09-13" version="1">MEGVMRAISALEKLTLTKEALEEEYDDGTRLGRLVNDIRKAATSNKLRDRCKRLIQKWKNQFVGAKGKSNPAEVSSPTTSQQNQNSASTQSPKVSPPVSKPPIKKGGTPVDQTKSENRKRRKPDSPAIPPDIQQPSKKQSVDKDSRKGISDSLKDSYKGSTEKKGPEVSSKDDNSATRNVSPKAGKAKSVRGREASKEKKLYKQVVQSSAIKAADENDTWQSGTKLKESKKRKSPKVAKRNFISKGNGSTSSSPTTYVQSEETQDSFVASPVSFFDEASQSPVSFSLQDDSVINSRAESSIFDITAATEGSIVDELTVSSVPDSPADSVHYKETLDSGESSQHSNDSNKMSEIVEDVRTLGDEDSLNEEDERFLKKEISEDDIRRLHLEHWEGVNGNYNSNRDWSSWNECVVRDSGGGNSLHILPYVCID</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C9B9</accession>
    <name>A0A9Q1C9B9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_12500</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C9B9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8041628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="116" mass="12981" checksum="7C0300464D575E0B" modified="2023-09-13" version="1">MPPFRLEGPVQSKMDEWDECTSVSSTAEQRKYPSEALKLPTFLPLEDHFCLSIVILPGGEKRKVLVSSFKRAPLPLLVLESQKGKFPGALTVWSQFKTVGMGSGLVRQSVSFLWQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CAH0</accession>
    <name>A0A9Q1CAH0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">28S ribosomal protein S17, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_12181</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CAH0"/>
    <dbReference type="OrthoDB" id="274752at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039193">
        <property type="entry name" value="Ribosomal_uS17m_metazoa"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24088">
        <property type="entry name" value="28S RIBOSOMAL PROTEIN S17, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24088:SF0">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US17M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8041380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="150" mass="17603" checksum="783C8DEE567A6E49" modified="2023-09-13" version="1">MAGHSRILLGQVVQSKLRDAAKVQITKLKLDTYVMQYFPDKSSVFAFDPKGVTKVGDIVLVKELEARRTRFITHKLEKIIYSIGNVRDPLTGKRCDSETYWDEEERKKLFEKEAEEQTGMEETKAEKRSVYEFERMKDPILSLKRKDWKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CAP9</accession>
    <name>A0A9Q1CAP9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_12042</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CAP9"/>
    <dbReference type="OrthoDB" id="5984501at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF26">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8041271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="217" mass="24985" checksum="A2046DFB03EB137D" modified="2023-09-13" version="1">MMFSAMMTDAFHGCGDGIHIRYRTDGGLFNPRRLLAVTKVKDTIIRDFLFADDCALSATTESSMQTKLQTVDQFTYQGSTFTTAINIDAEVNNRTAKASSAFGRLREKVWERRGISTTTKLKVYKAVVLTTLHYACENLTVYRRHANQLNHFHMSCLRRLLHIRWQDMVPDTEILRRANLQSIFTLLQKAQVRWAGHTVRLSDDRLPKQPLYGQWPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CAZ3</accession>
    <name>A0A9Q1CAZ3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Apolipoprotein D</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_12164</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CAZ3"/>
    <dbReference type="OrthoDB" id="565904at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012674">
        <property type="entry name" value="Calycin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022272">
        <property type="entry name" value="Lipocalin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000566">
        <property type="entry name" value="Lipocln_cytosolic_FA-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10612">
        <property type="entry name" value="APOLIPOPROTEIN D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10612:SF34">
        <property type="entry name" value="APOLIPOPROTEIN D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08212">
        <property type="entry name" value="Lipocalin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50814">
        <property type="entry name" value="Lipocalins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00213">
        <property type="entry name" value="LIPOCALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040500732" evidence="1">
        <location>
            <begin position="20"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Lipocalin/cytosolic fatty-acid binding" evidence="2">
        <location>
            <begin position="34"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8041364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="120" mass="13589" checksum="7EF2ABCDECC068AD" modified="2023-09-13" version="1">MAFHFITLSFLALFSPTLAINWGRCPSVPTVDDFDVTKYYGKWYEIARSDNIPFENGARCVTAEYTPTWSRRISVQNTGIVDGDDGPEVREAQGEAWIPDEDYPAQLRVKLSPCKYTVFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CCY3</accession>
    <name>A0A9Q1CCY3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">E3 ubiquitin-protein ligase rififylin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_09503</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8042682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CCY3"/>
    <dbReference type="OrthoDB" id="3045089at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902042">
        <property type="term" value="P:negative regulation of extrinsic apoptotic signaling pathway via death domain receptors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070936">
        <property type="term" value="P:protein K48-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15750">
        <property type="entry name" value="FYVE_CARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16500">
        <property type="entry name" value="RING-HC_CARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000110">
        <property type="entry name" value="E3 ubiquitin-protein ligase RNF34 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051728">
        <property type="entry name" value="RING-FYVE_E3_ubiquitin-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055111">
        <property type="entry name" value="RNF34_RFFL_HeH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057299">
        <property type="entry name" value="RNF34_RFFL_SAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036361">
        <property type="entry name" value="SAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14879">
        <property type="entry name" value="CASPASE REGULATOR, RING FINGER DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14879:SF15">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RIFIFYLIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22968">
        <property type="entry name" value="RNF34L-like_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23632">
        <property type="entry name" value="SAP_RNF34_RFFL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68906">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="407"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="158"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="226"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="16"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="158"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="184"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="226"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="245"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="265"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="295"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8042682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="454" mass="50404" checksum="24FAC7FF269B87BA" modified="2023-09-13" version="1">MGSSPSSQPGSNSQNHTTHSNQRTGQNRIPNQSEVFVAWDNHNSGQQRTQQRVHIQRNNDTMVCEACKANFTLLKRKYLCCDCKKHYCNACSAKEFKKCRNCLLFKLPCPREKLQQMKIKDLRQYLSVRDIPTDSCREKSDLVELTFNFLNSRSTNGPTGLFQGSANISQNRSGSGIRQPPPASGTQTGSMPNSPNATDWLYNRAHQQYFGGFAGANEGRMFGTSVSSGFASSQTAQPHGPFATSQEQYSSSSSRQQSSQHSTAEETNSQGSSATETGNQQATEENSQQGQPVSGQGTANTTNETENNTQARDPPPVRRRASLKDIDKVEDIEQLTNRQLKEILAANFVDYKGCVERYELSDRVKRLYEDREGMKAKGKYLDSVKEKAANAGEADTPGQQSGDDDLCRICMDATIDCVLLECGHMVTCTTCGKQMNECPICRQYVVRCVHIFRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CD59</accession>
    <name>A0A9Q1CD59_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Retrovirus-related Pol polyprotein from transposon TNT 1-94-like beta-barrel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_10139</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CD59"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054722">
        <property type="entry name" value="PolX-like_BBD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22936">
        <property type="entry name" value="Pol_BBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Retrovirus-related Pol polyprotein from transposon TNT 1-94-like beta-barrel" evidence="1">
        <location>
            <begin position="9"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF22936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8043166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="235" mass="26510" checksum="FB4F6A9F275A1643" modified="2023-09-13" version="1">MKHEVKGLLVDCGGTTYVVNDQSKFIRFDDFNPADHNIELATINNVAMGKGDASIMLVDSNGNAQKAIHKDALYIPSFKQDIFSVQVAADKGAFVQFKSNSAESGKVGSNVEQNREMEHHNDLFKEECRHFRGKVSVSSVNRVSHAAQPLEEILMKCDKASKVKSHRSKHTKKDRKDDVTNLAANFHTNEVFKCKPPRSHYAFPSCPINPLTQLNLIDFQDWLRDKVKNIIQRDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CD91</accession>
    <name>A0A9Q1CD91_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_09673</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8042816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CD91"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8042816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="139" mass="16122" checksum="7DDD9D1CB8A040C3" modified="2023-09-13" version="1">MESQTLAELDKKEQDPEVNGSLPEDGKEKDVVSSKMKSESKDDENHTSEKDSSASPEKRRKRKQSRHSRSASPEARSRSRSSERQSKSRKSRSRSRSRSRSRKSKKKHSPSRSRSRSRSKDKRLKRRKEATVLVEVDLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CDZ4</accession>
    <name>A0A9Q1CDZ4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Carboxypeptidase N subunit 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_10340</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CDZ4"/>
    <dbReference type="OrthoDB" id="2013775at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004180">
        <property type="term" value="F:carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001164">
        <property type="entry name" value="GH01279p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001438">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026906">
        <property type="entry name" value="LRR_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000372">
        <property type="entry name" value="LRRNT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366:SF168">
        <property type="entry name" value="GH22922P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366">
        <property type="entry name" value="IG(IMMUNOGLOBULIN) AND LRR(LEUCINE RICH REPEAT) DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13306">
        <property type="entry name" value="LRR_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="SMART" id="SM00013">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 9" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040306219" evidence="9">
        <location>
            <begin position="29"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="domain" description="LRRNT" evidence="10">
        <location>
            <begin position="33"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8043312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="502" mass="56814" checksum="AE81D74BEDB012D9" modified="2023-09-13" version="1">MKYLLMAMICSVSLEVVIISMSVASGEATAPSACEICKCHTEYDVFTIDCSNRNLSTVPKNIPANVTTLILTSNKIAELKVEDFSGTPNLTKLYLRKNMLTVVPPDLFANNQKLTDINLSGNKLRTIPETLFHHTPKLIRLSCSKNNLQEIPPDLFHSTQLIERIHLQENDIQYLPPGLFSHSRSVKEIYMSNNLLKHLNPDLFTNTSSLETVFFSHNNLQEISPDLFHPTPSIEKVFLNNNSLTTLPESLFAFAKILYQLNLSNNKLKSLPEKLFANTSKLISLNLSSNCLAYIPENLLPNTKKLQSLSFSSNMLESIPERIFHSTPKLKSLDFVYNRLTELPRDLFAAVEELKYLQMSANRIKILEKDLFRNVVLTNEMDLSNNSILSIPDGLFTSNEKSQLLKTLFLQRNKIRYIQKDAFKGLGELRLICLFDNQIEAIDSGAFSVESLKKIFLFKNNLQQITNISFSNSSLSEIHLYGNNITNITSNATEGLLSQPIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CE14</accession>
    <name>A0A9Q1CE14_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_10233</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CE14"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8043236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="71" mass="8910" checksum="2FBBAE78C52A0E69" modified="2023-09-13" version="1">MVWIRKRQMCPISLFRTRIMLIWPNYQFTSQVSRRSTGTVHLHFELHRLCMSVFARYIFHWTYIEIFIKMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CED7</accession>
    <name>A0A9Q1CED7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_10492</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CED7"/>
    <dbReference type="OrthoDB" id="10068084at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="134"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="62"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="146"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="158"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8043416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="167" mass="18878" checksum="BF51B38BC5C2C1DB" modified="2023-09-13" version="1">MFMHSVSSGLSDADIRQEMKPLLLHGKLSDEKLFEKLNQVVYREMERKKKMQHKHHFVTPVEVKDEPGASGSTEKKKEKKEGNSLYEEVQLLKLQVAEIHQALTPGRQNPTPQLRQRGCSKCQQANKGDTCKHCYKCGKFGHFARGCRQTGNDQGSPLRDEGSPAAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CEW1</accession>
    <name>A0A9Q1CEW1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Retrotransposon gag domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_10462</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CEW1"/>
    <dbReference type="OrthoDB" id="5974147at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33198">
        <property type="entry name" value="ANK_REP_REGION DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33198:SF20">
        <property type="entry name" value="RETROTRANSPOSON GAG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8043400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="249" mass="28193" checksum="A427A83551242101" modified="2023-09-13" version="1">MSVNDEANESSQANQIFDMNVSAPQTPQPRPLTNIAPPPPIQAKEGAAEEWKLFKQMFEGYCIITQLHSHDAVYQKAVFLHCIGPVGVKIYNSLTFADDEDKNDIKTIMTKLDTYFIGESNETYERYVFNKRDQQAGETVDAYVANLRTLAKTCNFGDLEESLIKDRLVLGINDPGTRKTLLQTRNLTLQDAVDICKTDEATRNQLKSIDTESVNKVHQKKGIGQRRDKKSVPMIHNCKFCGRSHPKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CFH0</accession>
    <name>A0A9Q1CFH0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_10907</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ADP-ribosyl cyclase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CFH0"/>
    <dbReference type="OrthoDB" id="10028716at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061809">
        <property type="term" value="F:NAD+ nucleosidase activity, cyclic ADP-ribose generating"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016849">
        <property type="term" value="F:phosphorus-oxygen lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.82.10">
        <property type="entry name" value="ADP Ribosyl Cyclase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003193">
        <property type="entry name" value="ADP-ribosyl_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10912">
        <property type="entry name" value="ADP-RIBOSYL CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10912:SF7">
        <property type="entry name" value="ADP-RIBOSYL CYCLASE_CYCLIC ADP-RIBOSE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02267">
        <property type="entry name" value="Rib_hydrolayse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52309">
        <property type="entry name" value="N-(deoxy)ribosyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8043700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="324" mass="35864" checksum="F888D32618ED1375" modified="2023-09-13" version="1">MVVILRKLNSVIFLLILPFIGNFYEVRGHIVRRSTSPQGTTKFLSKIFSGRCNDYSDCTLAHTSQCNTYPSRSQTNCTDALNEFLEAFAFKDPCKVPEDAYDGFLNKSMVHSEPNETLLWSGTKNIAINIATITDRYTTIERTAAGYILNGLRWCGENGSSGINYGSCGPCEEGQYDASTQFWRSASRHFAAQARGFVSVVLNSTRNGGAFNETSIFASQELPNINVTLVSHVYIHVVRSVTTPDNITGEDCDGASIMKLKARLTDKGLNHSCSFNDREFILVQCVQYPDAAPCQMLSSSPVALKRSNILFILSLFTLLINVKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CG42</accession>
    <name>A0A9Q1CG42_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ankyrin-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_11460</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8044093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CG42"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051860">
        <property type="entry name" value="Plasmodium_CSP_Invasion"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44826:SF5">
        <property type="entry name" value="DYNEIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44826">
        <property type="entry name" value="SPORE COAT PROTEIN SP85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="16"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="42"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="58"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="106"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8044093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="241" mass="27332" checksum="A14A0C33FDD07E49" modified="2023-09-13" version="1">MKTFNRSIHQSTNPTIHPSTHSLIHPSTHPPIHPFTHPPIHPSTHSPIHPFTHPPIHPSTHSPIHPSTHSPIHPSTHSPIHPSTHPPIHPFTHPPIHPFTHPPIHPSTHSPIHPSTHSPIHPSTHPPIHPFTHSPIHPFTYPPIHPIHPTVLSVLLSIRQSILSRHTPFNKTCNVCLSLSVDLSLLERWKGEVLLNSFLSCMTISQAFYNKDIMTLFFLFCFRTKIILNKLSRDYRGLCNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CIR5</accession>
    <name>A0A9Q1CIR5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_04807</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CIR5"/>
    <dbReference type="OrthoDB" id="5973769at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030133">
        <property type="term" value="C:transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028066">
        <property type="entry name" value="TMEM187"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15066">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 187"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15066:SF0">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 187"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15100">
        <property type="entry name" value="TMEM187"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="93"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="120"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="153"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="221"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8046202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="246" mass="27790" checksum="EB0D5B295CE136DD" modified="2023-09-13" version="1">MTEIVSATFLPVLVVASILALSVYLDLYNSVYVELGFEHYAEKANSTLGGYKFPVWMKMPANTLVNVGYVGVGFLWLLRVTSSDKLHETDIATNLYMVSIFAWMAIIYGFTQFARIVTQSLFWAVLDQWFTLPFFCWTIIWSRTVQHGWKAQSAVLILVLSILSYNLALLHKFGFDVALGVHIAVAFWHGSSVVRRDQVANLENYLDEAGEFASVFSSESSWALVFGCGVWKPIVVYVCFEHHQGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CJI5</accession>
    <name>A0A9Q1CJI5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Reverse transcriptase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_08661</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09274">
        <property type="entry name" value="RNase_HI_RT_Ty3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22249">
        <property type="entry name" value="UDM1_RNF168_RNF169-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000115">
        <property type="entry name" value="Polyprotein of retroviral origin, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.370:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 17.6-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000032">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 297-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon gypsy-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054465">
        <property type="entry name" value="Integrase_p58-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041373">
        <property type="entry name" value="RT_RNaseH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22938">
        <property type="entry name" value="Integrase_p58_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17917">
        <property type="entry name" value="RT_RNaseH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="9">
        <location>
            <begin position="882"/>
            <end position="1040"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase" evidence="8">
        <location>
            <begin position="1296"/>
            <end position="1473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="107"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="351"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="616"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="715"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="351"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="366"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="390"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="616"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="639"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="659"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="684"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="731"/>
            <end position="755"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8045619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1688" mass="193304" checksum="E1D4A96A6FF77D85" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CNS1</accession>
    <name>A0A9Q1CNS1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">GDH/6PGL endoplasmic bifunctional protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_00287</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; pentose phosphate pathway.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CNS1"/>
    <dbReference type="OrthoDB" id="60984at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017057">
        <property type="term" value="F:6-phosphogluconolactonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004345">
        <property type="term" value="F:glucose-6-phosphate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009051">
        <property type="term" value="P:pentose-phosphate shunt, oxidative branch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01400">
        <property type="entry name" value="6PGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005900">
        <property type="entry name" value="6-phosphogluconolactonase_DevB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001282">
        <property type="entry name" value="G6P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022675">
        <property type="entry name" value="G6P_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022674">
        <property type="entry name" value="G6P_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006148">
        <property type="entry name" value="Glc/Gal-6P_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01198">
        <property type="entry name" value="pgl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23429:SF7">
        <property type="entry name" value="GDH_6PGL ENDOPLASMIC BIFUNCTIONAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23429">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE 1-DEHYDROGENASE G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02781">
        <property type="entry name" value="G6PD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00479">
        <property type="entry name" value="G6PD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01182">
        <property type="entry name" value="Glucosamine_iso"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00079">
        <property type="entry name" value="G6PDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="2" id="KW-0313">Glucose metabolism</keyword>
    <keyword evidence="3" id="KW-0521">NADP</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucose-6-phosphate dehydrogenase NAD-binding" evidence="5">
        <location>
            <begin position="41"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="Glucose-6-phosphate dehydrogenase C-terminal" evidence="7">
        <location>
            <begin position="228"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="domain" description="Glucosamine/galactosamine-6-phosphate isomerase" evidence="6">
        <location>
            <begin position="568"/>
            <end position="794"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8048113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="803" mass="91444" checksum="E34D2DD6CB8853B2" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CRL9</accession>
    <name>A0A9Q1CRL9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_04129</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the peptidase S8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CRL9"/>
    <dbReference type="OrthoDB" id="206201at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04077">
        <property type="entry name" value="Peptidases_S8_PCSK9_ProteinaseK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000014">
        <property type="entry name" value="Proteinase K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.80">
        <property type="entry name" value="Peptidase S8 propeptide/proteinase inhibitor I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034193">
        <property type="entry name" value="PCSK9_ProteinaseK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050131">
        <property type="entry name" value="Peptidase_S8_subtilisin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010259">
        <property type="entry name" value="S8pro/Inhibitor_I9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037045">
        <property type="entry name" value="S8pro/Inhibitor_I9_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806:SF58">
        <property type="entry name" value="ALKALINE PROTEASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806">
        <property type="entry name" value="PEPTIDASE S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05922">
        <property type="entry name" value="Inhibitor_I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5 6" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0720">Serine protease</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040159010" evidence="7">
        <location>
            <begin position="16"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="Inhibitor I9" evidence="9">
        <location>
            <begin position="31"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="8">
        <location>
            <begin position="129"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="138"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="173"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF05922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8050792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="377" mass="39410" checksum="2DBDCA73D8D970CB" modified="2023-09-13" version="1">MKFVLITLLAAAASASFAPLYTDKERIDGSYIIVLKEGVSLTSTLRSLENDPYFTTFGGRIEKVYGNVLNGFSASLGEKALEYIRQLNIVSYVEEDGMVYAQAVESWGLDRVDQRNLPLNDGFTPRGTGAGAHVYVIDTGIRESHNDFGGRASIATDTCRTPNVSNGVDCHGHGTHCAGTVGGKDYGVAKSASLYGVRVLNCQGSGYKSDTIAGMNWVKANRNNPAVVSMSLGGSASTAQDDAVADLDADGVIVIVAAGNDNSDACNGSPSRAEKAFTVGATDNSDARASYSNYGSCVNIFAPGSSIKSAWYLTDSSSRTISGTSMATPHVSGAAAVLLGIDNTLTPANVKNKLETEATSNCVSDVQGSPNKMLYIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CT46</accession>
    <name>A0A9Q1CT46_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SCAN box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_03664</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CT46"/>
    <dbReference type="OrthoDB" id="8963689at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF13">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCAN box" evidence="2">
        <location>
            <begin position="165"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8050453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="258" mass="31205" checksum="F9E7A55BD290E740" modified="2023-09-13" version="1">MRKDEIKRYVLEYLVDESILPSTVLETAITVPTDNTFELKRLEMEMDKEIRLKEREREESEEREEREMQMQKEKEEREMQREKEAREHEFRLKQLELGEDNVEKFFNHFEKLGEQLKWPRDKWSILIQSNFTGKAQEVYSALSIEDSMDYDKVKKAILQAYELVPEAYRQKFRKYRKADTQTYVEFAYQKERHFDRWCASKKVSTFDALRQLMLVEEFKRCVNDDIKTHLEENKADKLSEVANLADQYALTHKFSKVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1GVV7</accession>
    <name>A0A9Q1GVV7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_32860</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1GVV7"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF13">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="78"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8027657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="332" mass="39514" checksum="51E7FC6A992F3837" modified="2023-09-13" version="1">MPFNLDKFVASPSVEELDSLKKSEIVKVAKHYGIEFQPLMRKDEIKRYVLEYLVDESVLPSTVLETAITVPTDNTFELKRLEMEMNKEIRLKEMEREREREKEAREHEFRLKQLELGVIKASDPKIGLDTGGFDVSKHVKFVPKFQEDNVEKFFNHFEKLGEQLKWPRDKWSILIQSNFTGKAQEVYSALSIEDSMDYDKVKKAILQAYELVPEAYRQKFRKYRKADTQTYVEFAYQKERHFDRWCASKKVSTFDTLRQLMLVEEFKRRPKLFQGGECYKTTRQKHRIESIDERMESSREFNFNAAFLELSRSENCLRYVAITTTVCSRSFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1GXJ0</accession>
    <name>A0A9Q1GXJ0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Organic cation transporter protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_32706</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1GXJ0"/>
    <dbReference type="OrthoDB" id="6612291at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17317">
        <property type="entry name" value="MFS_SLC22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24064">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="238"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="263"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="291"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="319"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="437"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="465"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="497"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="519"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="552"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="581"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="183"/>
            <end position="604"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8027539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="668" mass="75649" checksum="81E5688994A4EF87" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1GYU8</accession>
    <name>A0A9Q1GYU8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Rab protein geranylgeranyltransferase component A 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_29943</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8027868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1GYU8"/>
    <dbReference type="OrthoDB" id="1923006at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005968">
        <property type="term" value="C:Rab-protein geranylgeranyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005092">
        <property type="term" value="F:GDP-dissociation inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.519.10">
        <property type="entry name" value="Guanine Nucleotide Dissociation Inhibitor, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018203">
        <property type="entry name" value="GDP_dissociation_inhibitor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001738">
        <property type="entry name" value="Rab_escort"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054420">
        <property type="entry name" value="RAE1_2_domI_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11787:SF4">
        <property type="entry name" value="CHM, RAB ESCORT PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11787">
        <property type="entry name" value="RAB GDP-DISSOCIATION INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22603">
        <property type="entry name" value="RAE1_2_domI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00893">
        <property type="entry name" value="RABESCORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RAE1/2" evidence="5">
        <location>
            <begin position="62"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="211"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="287"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF22603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8027868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="320" mass="35075" checksum="8875E0C65D3C2105" modified="2023-09-13" version="1">MSAVFGGIYMLRTTADYLIVDDTNVCRGMIDSEGRRLTCKNVIMDHSYTTTHHEGIQSDRFVSRAVLITDKTVRESENEEVTFLTIPPPKPAEEPVRVLEIGHTACASPKGTYVVHLTRKCSSPDAKTDLMPAVELLCQSLEAELDYSCEKPKVLWSMFFSYQDKSDVSLEDLRAGQPGGVHVTGGPGCSVGYEHAVKQAKQIFEQICPGEEFLPPAPNPEDIIYDEPSGDNPGHSNKAGFEDNPGDELKPEESRSGNGDQFSEQEIHKCEDEGGDDSPTPEAAENVGKESSTKDENQTETVLVESISKEYPQGQGGQEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1GZG3</accession>
    <name>A0A9Q1GZG3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_29333</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1GZG3"/>
    <dbReference type="OrthoDB" id="425014at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF26">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8029832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="218" mass="25500" checksum="BF9BDA23A81B5FC0" modified="2023-09-13" version="1">MPSYRHIKKLTNFHMVCLRKILNIRWIEKVPDTEVQERAGLPSISALVRKAQLRWIGHVVRTPESRMPKKKLYLELKRHPEGQLKRFKDNLNASLKACSIPLYICEVLAADRSNYRLLTHMGVTYYETCRVTSAKEKRALRKSKFIHLYIYPTRMSQMSKDISSSNWNRLSFTDAPKGSIAEVVLSSLFLFLRFVLSSSISMDNQEEKEEYVFVTLRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1GZP4</accession>
    <name>A0A9Q1GZP4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Neuronal acetylcholine receptor subunit alpha-10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">HOLleu_29516</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">Nanhai2018</strain>
            <tissue evidence="19">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Synaptic cell membrane</location>
            <topology evidence="14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1GZP4"/>
    <dbReference type="OrthoDB" id="5975154at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022848">
        <property type="term" value="F:acetylcholine-gated monoatomic cation-selective channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18997">
        <property type="entry name" value="LGIC_ECD_nAChR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19051">
        <property type="entry name" value="LGIC_TM_cation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000073">
        <property type="entry name" value="Neuronal acetylcholine receptor subunit alpha-9-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000016">
        <property type="entry name" value="Nicotinic acetylcholine receptor subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002394">
        <property type="entry name" value="Nicotinic_acetylcholine_rcpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00860">
        <property type="entry name" value="LIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00254">
        <property type="entry name" value="NICOTINICR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13 15" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="8 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 19" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0770">Synapse</keyword>
    <keyword evidence="4 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 15" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="201"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="232"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="262"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="416"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="17">
        <location>
            <begin position="2"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="18">
        <location>
            <begin position="208"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="372"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00034099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAJ8029967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="443" mass="50002" checksum="210E4DC424192744" modified="2023-09-13" version="1">MLGNYSTTVRPVARPEDFVPVKLGAALQQIIDMDEKNQVITLNLWMRFQWTDASIHWDPADFGGTTFMIVPISSIWRPDIVLYTNADENFSGTMETSATITNEGVVYWNAPAIYKSTCKIDVTYFPFDEQHCDLKFGSWAYSGDELSLTNRSVSGDTSSYINNGEWELVGMPVSNNIVYYACCASPYPDVTFTVIIRRLPLFYMFNLIGPCIVISLVTTLAFYLPADAGEKVTLGITVLLALVVFLLLAAETMPPTSEVVPIVGQYFASTIILVTISIFITVYILSLHYRLPGTRPVPKYLKKIALVYLARILCMDNAIKVSDISSEENETNVMENNHHADHTEINQQPTQKENNLQGQRPISALSNLRVGANRFEESLTKIKKNLDFLTQRAKDTDSEDDIMNEWKQVAHVFDRFLLWLFLVLTIVITTIVLAQKKPFTELE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-04-02" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H023</accession>
    <name>A0A9Q1H023_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">TP53-binding protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_28122</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040134884" evidence="1">
        <location>
            <begin position="21"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8030104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="148" mass="17351" checksum="153B60A29D714DE3" modified="2023-09-13" version="1">MKIPMKLLELWPMMLVSVSQWLILVSHIEWGGRPRANQNNQVAPRQIIAKFVRREVKSQILKNKKVLRTKPERANVYLDEDLTPLRARICKELRKEPGRRVWTMDGRIFTSITKEGKEVKHVLDKANDFLLLGWSEQKIQEVGLLLDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H1T1</accession>
    <name>A0A9Q1H1T1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_26731</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H1T1"/>
    <dbReference type="OrthoDB" id="10068084at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="329"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="257"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8030344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="358" mass="40281" checksum="92F0152448A914CA" modified="2023-09-13" version="1">MSLTVEGENGTDGAEADVGDDPEKLSKTIKALQDQLKRLQSNLPEEFISEEKPDSPLGLKVQKELKISGQIGNPGQKDKLSFVSLIHQIEGAIKKGYSEREVVAAVVKAVTPGLQLRNYLEGRSDLSPILRRLLRAHFQEKSSTELYQELCNIAQGPRETAQSFLLRCLALKERILFASKEEDAEFKYESSLVEAMFMHSVSTGLADADIRQEMKPRLLHGKLSDEELFEKLNQVVYREMERKKKMQQKHHFVTPVEVKDEPGASGSAEKKKEKKEGNSLYEEVQLLKLQVAEIHQALTSGWQNPTPQLRQRGCSKCQQANKGDTCKHCYKCGKFGHFARGCRQTGNEQGSPLRDEGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H2K5</accession>
    <name>A0A9Q1H2K5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_26553</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H2K5"/>
    <dbReference type="OrthoDB" id="10477327at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8030210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="63" mass="7359" checksum="7B0E5E4314DF7748" modified="2023-09-13" version="1">MKPLWKAEAIDSDAGNDSEIAYRLAEVAGYDWLIFRVHNYTGDIFYEGDPPDWSEHPQFRVRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H3A3</accession>
    <name>A0A9Q1H3A3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_24051</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H3A3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="38"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8033717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="133" mass="15308" checksum="F767C17E27FC7E76" modified="2023-09-13" version="1">MDFKGITLGAFYTVTGIAMARATHFDNEDTRKERKEFLPWWVYLLFVVALIFIQYVVRLLIRSCRAKKENHSNLNDEDPVRTEHRPKESAPPSYIEVTERTQLDAHSLACPPPPSYDVAMNLCQLSRDGPVLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H487</accession>
    <name>A0A9Q1H487_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Aldose reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_25266</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldo/keto reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H487"/>
    <dbReference type="OrthoDB" id="416253at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43827">
        <property type="entry name" value="2,5-DIKETO-D-GLUCONIC ACID REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43827:SF3">
        <property type="entry name" value="NADP-DEPENDENT OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00063">
        <property type="entry name" value="ALDOKETO_REDUCTASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="4">
        <location>
            <begin position="36"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8031905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="218" mass="24910" checksum="E236B2A07BED45FA" modified="2023-09-13" version="1">MIHRCSMTYTLRKLGKKRKTSQGTFATASKFHSFLLSKAMEHLVEKGLVNSIGVSNFNISQMEEVLKIAKKPVVVNQVENHPCIDQRELIDFCKSKNILVTAYSPLGSPDRSWTTEKDPNIQEDPVVKKIAEHKGCTPVQLLIAYNLCQGLLCIPKSVTPSRIRQNFQALGVQLTKEEIEKLDTLVTDYRACAWEFWSHHPYYPFPEEVEKLSDEMIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H4C4</accession>
    <name>A0A9Q1H4C4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Zinc finger MYM-type protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_22973</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8032894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H4C4"/>
    <dbReference type="OrthoDB" id="427960at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025398">
        <property type="entry name" value="DUF4371"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006580">
        <property type="entry name" value="Znf_TTF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45749">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45749:SF21">
        <property type="entry name" value="DUF4371 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14291">
        <property type="entry name" value="DUF4371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00597">
        <property type="entry name" value="ZnF_TTF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="46"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="200"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="324"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="227"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="239"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="272"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8032894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="714" mass="79334" checksum="BFC9CEF2A9DF63C8" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H5V2</accession>
    <name>A0A9Q1H5V2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">THAP domain-containing protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_24354</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the THAP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H5V2"/>
    <dbReference type="OrthoDB" id="5982876at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026516">
        <property type="entry name" value="THAP1/10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006612">
        <property type="entry name" value="THAP_Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46600">
        <property type="entry name" value="THAP DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46600:SF1">
        <property type="entry name" value="THAP DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05485">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00980">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50950">
        <property type="entry name" value="ZF_THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="THAP-type" evidence="13">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ8033958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="172" mass="19685" checksum="6DC64F3275C32A73" modified="2023-09-13" version="1">MGRKCAIKSCQSRRSSDRAVSGEITFHRFPKAKTEEWMKATGLLGEELSPETTLCSEHFEKDCFAIIRTGRRCLLKNSIRTKNLGQVQYYRVPSLEKHNYYKGCKMEPFPLTNCLTPFLRCVLRLLSSIIASLVVIRSWNQFLRYSTLQVYHCAIVNVHALPALGPDQANIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H609</accession>
    <name>A0A9Q1H609_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_17465</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8036822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H609"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8036822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="82" mass="9406" checksum="CA21242DD99F9F56" modified="2023-09-13" version="1">MTLVRLYSTMRDLCHSLQDVVSVLESQSLKLNYFCCFHGSFNISNSKKHLAWRTSATLRGTHWLLPTCPNHTKSSLRNASHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H6T1</accession>
    <name>A0A9Q1H6T1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_21891</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H6T1"/>
    <dbReference type="OrthoDB" id="6144240at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8034873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="299" mass="34941" checksum="04CF72DDED646626" modified="2023-09-13" version="1">MRYTQPFDYQHYVSSAHALQNILYATRSRNWHLIDYVIIRKDDRNDIRVTKAMCGAYCWTDQQLIISKLNLHIKPRKRPQGQRTPRRLDISKLKVEEVAKNLSSALEQRIMESTNVQHNIEEQWASVRDILYSTALEHLGSAKRKHQDWFNDNKEVIQSLLSEKHRLLKEYQNDPSSTSNKTAFNNIRRTIQTELRNMQDLWLSKKAHEIQTFADSNNVRCSYEALNIVYGPRASGSSPVLNADGTKLLTDRKQILERWAEHFNSVLNRPSSINNDTIDRLPQVKTSYTLDDLPIEHEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H7N5</accession>
    <name>A0A9Q1H7N5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">methylcrotonoyl-CoA carboxylase</fullName>
            <ecNumber evidence="3">6.4.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">3-methylcrotonyl-CoA carboxylase 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">3-methylcrotonyl-CoA carboxylase non-biotin-containing subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">3-methylcrotonyl-CoA:carbon dioxide ligase subunit beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_20015</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>3-methylbut-2-enoyl-CoA + hydrogencarbonate + ATP = 3-methyl-(2E)-glutaconyl-CoA + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13589"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57344"/>
            <dbReference type="ChEBI" id="CHEBI:57346"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.4.1.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid degradation; L-leucine degradation; (S)-3-hydroxy-3-methylglutaryl-CoA from 3-isovaleryl-CoA: step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the AccD/PCCB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.4.1.4" evidence="3"/>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H7N5"/>
    <dbReference type="OrthoDB" id="439921at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905202">
        <property type="term" value="C:methylcrotonoyl-CoA carboxylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004485">
        <property type="term" value="F:methylcrotonoyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006552">
        <property type="term" value="P:L-leucine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000004">
        <property type="entry name" value="Methylcrotonoyl-CoA carboxylase beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000007">
        <property type="entry name" value="Methylcrotonoyl-CoA carboxylase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034733">
        <property type="entry name" value="AcCoA_carboxyl_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011763">
        <property type="entry name" value="COA_CT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011762">
        <property type="entry name" value="COA_CT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045190">
        <property type="entry name" value="MCCB/AccD1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22855">
        <property type="entry name" value="ACETYL, PROPIONYL, PYRUVATE, AND GLUTACONYL CARBOXYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22855:SF13">
        <property type="entry name" value="METHYLCROTONOYL-COA CARBOXYLASE BETA CHAIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01039">
        <property type="entry name" value="Carboxyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50989">
        <property type="entry name" value="COA_CT_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50980">
        <property type="entry name" value="COA_CT_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CoA carboxyltransferase N-terminal" evidence="8">
        <location>
            <begin position="50"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="CoA carboxyltransferase C-terminal" evidence="9">
        <location>
            <begin position="307"/>
            <end position="556"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00025711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00026116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00031109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00031237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00052347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8036134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="564" mass="61466" checksum="0E1DBBE1AD7B9088" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H9T0</accession>
    <name>A0A9Q1H9T0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_18346</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H9T0"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8037516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="106" mass="12530" checksum="D2DBCE5FDA19A9D5" modified="2023-09-13" version="1">MAIQNRQEEILTGTDCIKNFMASARLAATDCDFLEEHLYSICPSRIAAHRFPLKRNYQLICVISYLTYLLKEGRVQYFYICQDLKVLLHVYPPKEEHFEHFQADLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HA39</accession>
    <name>A0A9Q1HA39_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Leucine-rich repeat-containing protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_18707</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HA39"/>
    <dbReference type="OrthoDB" id="28057at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050541">
        <property type="entry name" value="LRR_TM_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000372">
        <property type="entry name" value="LRRNT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369">
        <property type="entry name" value="ANTIGEN BSP, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369:SF212">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 4B-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00013">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040223274" evidence="6">
        <location>
            <begin position="28"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="406"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="domain" description="LRRNT" evidence="7">
        <location>
            <begin position="29"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="LRRCT" evidence="8">
        <location>
            <begin position="291"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="375"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="375"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8037801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="470" mass="52598" checksum="16FBC7F2E376E135" modified="2023-09-13" version="1">MAVREFWLRWPTSTVLVLLLWCSFVRAQTCPKGCKCEGLSVTCANGLMQYVPPGIPINTTHLNFSNNSLTIIRNQTQLHNLPNLRTLSLWKNRIIAIEDGAFWGHSALKNLDLSYNDLRIVNRSAFRGLPGLNTLDLHGNPRLCLDFGSLGTLPNLQRLVLGYTNVIFIPGLFQTSSGNLNPPVGLLDLQLWEMGLLRIPYEAIKELPNLSELNMSQNKIKCIHHFAFTSNGNLTLLDLSSNPIAIVHSDAFEGLAKLSTLLLDNTNTLTMLPEGVFDPLPNGVKICLYGNKWNCDCHIKWLKSWTEEEGRVHCAQKPNCHTPSDLQGNPFFTVPKELFQCDGSVLNLPNVACYSDVKYTDGFCLELQQKHNIPMTTTTSSPSTDPITKKKVSTPTLSPTMHQSKIGFIVTGVSIMLIVILIVGVFIARQIRIQKKLHDQNKYFNISYENGDSQLQMAESPTPEKEKVFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HC83</accession>
    <name>A0A9Q1HC83_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Integrase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_11292</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HC83"/>
    <dbReference type="OrthoDB" id="10064731at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="CDD" id="cd22249">
        <property type="entry name" value="UDM1_RNF168_RNF169-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon gypsy-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF13">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase zinc-binding" evidence="3">
        <location>
            <begin position="767"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="308"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="573"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="674"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="97"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="308"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="324"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="348"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="573"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="597"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="642"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="689"/>
            <end position="706"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF17921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8043962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="833" mass="95077" checksum="70BFE3FB07F075D1" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-10-08" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HD21</accession>
    <name>A0A9Q1HD21_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Deleted in malignant brain tumors 1 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HOLleu_15260</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Nanhai2018</strain>
            <tissue evidence="17">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="536948at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd07066">
        <property type="entry name" value="CRD_FZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000001">
        <property type="entry name" value="Lysyl oxidase 4 isoform X1"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000005">
        <property type="entry name" value="Neurotrypsin isoform A"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000006">
        <property type="entry name" value="neurotrypsin isoform X2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000016">
        <property type="entry name" value="Scavenger receptor cysteine-rich protein type 12"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000007">
        <property type="entry name" value="Soluble scavenger receptor cysteine-rich domain-containing protein SSC5D"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000026">
        <property type="entry name" value="Tequila, isoform D"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.2000.10">
        <property type="entry name" value="Frizzled cysteine-rich domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020067">
        <property type="entry name" value="Frizzled_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036790">
        <property type="entry name" value="Frizzled_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19331:SF465">
        <property type="entry name" value="EGG PEPTIDE SPERACT RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19331">
        <property type="entry name" value="SCAVENGER RECEPTOR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01392">
        <property type="entry name" value="Fz"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00530">
        <property type="entry name" value="SRCR"/>
        <property type="match status" value="41"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00258">
        <property type="entry name" value="SPERACTRCPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00063">
        <property type="entry name" value="FRI"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="41"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63501">
        <property type="entry name" value="Frizzled cysteine-rich domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="41"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50038">
        <property type="entry name" value="FZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00420">
        <property type="entry name" value="SRCR_1"/>
        <property type="match status" value="26"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="41"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040429481" evidence="13">
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            <end position="2240"/>
        </location>
    </feature>
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        <location>
            <begin position="2341"/>
            <end position="2351"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="2454"/>
            <end position="2464"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="2564"/>
            <end position="2574"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="2673"/>
            <end position="2683"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="2782"/>
            <end position="2792"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="2890"/>
            <end position="2900"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="2998"/>
            <end position="3008"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="3106"/>
            <end position="3116"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="3214"/>
            <end position="3224"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="3322"/>
            <end position="3332"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="3429"/>
            <end position="3439"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="3535"/>
            <end position="3545"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="3644"/>
            <end position="3654"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="3751"/>
            <end position="3761"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="3870"/>
            <end position="3880"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="4388"/>
            <end position="4398"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="4697"/>
            <end position="4707"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="4801"/>
            <end position="4811"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="4906"/>
            <end position="4916"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="5011"/>
            <end position="5021"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ8040843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="5045" mass="542849" checksum="EA9E0C2FE3F31095" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HF60</accession>
    <name>A0A9Q1HF60_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Acyl-CoA dehydrogenase family member 10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_09701</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8042838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HF60"/>
    <dbReference type="OrthoDB" id="434771at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05154">
        <property type="entry name" value="ACAD10_11_N-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02603">
        <property type="entry name" value="HAD_sEH-N_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Acyl-CoA dehydrogenase fadE12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000018">
        <property type="entry name" value="Acyl-CoA dehydrogenase family member 10"/>
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    </dbReference>
    <dbReference type="Gene3D" id="3.90.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ACAD10-like"/>
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    <dbReference type="InterPro" id="IPR041726">
        <property type="entry name" value="ACAD10_11_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002575">
        <property type="entry name" value="Aminoglycoside_PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011945">
        <property type="entry name" value="HAD-SF_ppase_IA/epoxid_hydro_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02247">
        <property type="entry name" value="HAD-1A3-hyp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47829:SF3">
        <property type="entry name" value="AMINOGLYCOSIDE PHOSPHOTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47829">
        <property type="entry name" value="HYDROLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G12880)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01636">
        <property type="entry name" value="APH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00413">
        <property type="entry name" value="HADHALOGNASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoglycoside phosphotransferase" evidence="8">
        <location>
            <begin position="305"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="10">
        <location>
            <begin position="669"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="9">
        <location>
            <begin position="799"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="7">
        <location>
            <begin position="912"/>
            <end position="1060"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8042838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1070" mass="119080" checksum="DDFD57FC4E96BBB8" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HF81</accession>
    <name>A0A9Q1HF81_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PID domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_11543</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8044159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HF81"/>
    <dbReference type="OrthoDB" id="10056768at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046627">
        <property type="term" value="P:negative regulation of insulin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042127">
        <property type="term" value="P:regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051881">
        <property type="term" value="P:regulation of mitochondrial membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039112">
        <property type="entry name" value="PID1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16265">
        <property type="entry name" value="PTB-CONTAINING, CUBILIN AND LRP1-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16265:SF1">
        <property type="entry name" value="PTB-CONTAINING, CUBILIN AND LRP1-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14719">
        <property type="entry name" value="PID_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00462">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PID" evidence="2">
        <location>
            <begin position="86"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="240"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="311"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="400"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="263"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="318"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="343"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="412"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8044159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="421" mass="47630" checksum="7CCDE283F97BF907" modified="2023-09-13" version="1">MSFFRKKKNEVDFADAEKAIDREIAKGKQLAEKARAEEEPDLKEGEVRVRDIDTQHKRVVSEETSKERNRLKYKDTSGLVTSQERNNYPTFNVFYLGRIPTSAEYGKEAVEGPVDQLCKLRDKQKLPKVTVTFDTEGLSIKEVSSSLFSKTKNKKDGIDIYVPLHHITYGVASECHPSVFACIARMADDDTFLDDGMPVLMLHAFICEKPDIAQQITYWQLQAYIEAYEELKRKKILRNRRKQALGSRKSASFKKSPNHDTVPPLPPQPLPLYGNEPPPPLDLNEEGETDEPVYAQPKKGYKVKNKGFVKSKASNGHVPEDHAPDDNAKLASPTEVEPSSPKQVSRSDSVASDDSQSGPRDIAFERRISEMNELMVEMDAEKLKKLMISNHATLAREIYFSKSNNNRKSIDENEETDGSHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HFJ7</accession>
    <name>A0A9Q1HFJ7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_10228</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HFJ7"/>
    <dbReference type="OrthoDB" id="10045355at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8043231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="214" mass="24631" checksum="EE013BB2FBD442C1" modified="2023-09-13" version="1">MLGFSQGFTANYFCRFCRGHRQILQKQVTQDENLLRTKENYEDDLEQNDLSLTGIREPTVLNNLDKFHVIENVIPDVMHDFLEGIIPLEMFLVLSRLVEKEMITLEELNSRISCFGYGFIEQKNRPSPIKHTSILNPTKASGQTASQMMCSAPLLPLMIGDQIEDDCDEWALYLLLIDIFKIVMSPSLSLSSTYVLKALITDINYFYNYFQIAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HGD7</accession>
    <name>A0A9Q1HGD7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Mitochondrial potassium channel ATP-binding subunit</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">ATP-binding cassette sub-family B member 8, mitochondrial</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Mitochondrial sulfonylurea-receptor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">HOLleu_01380</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">Nanhai2018</strain>
            <tissue evidence="20">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HGD7"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015421">
        <property type="term" value="F:ABC-type oligopeptide transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090374">
        <property type="term" value="P:oligopeptide export from mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000836">
        <property type="entry name" value="ABC transporter B family member 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394">
        <property type="entry name" value="ATP-DEPENDENT PERMEASE MDL1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394:SF17">
        <property type="entry name" value="MITOCHONDRIAL POTASSIUM CHANNEL ATP-BINDING SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0630">Potassium</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transmembrane type-1" evidence="19">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="18">
        <location>
            <begin position="158"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00040439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00041416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00042968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAJ8048882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="384" mass="41396" checksum="1512E5E77BB044C2" modified="2023-09-13" version="1">MFSIAVHGDAEEVSRAMSVADEALANIRTVRAFAMEPQEGALFETEVKKAERLNSVLGCGIGIFQGFSNLFLNGLVLGVVYCGGYLMSSGTLQAGQLMSFLVAAQSIQRSIATMSILFGQAVRGISAGGRVFEFIKLEPSVRLSGGITIPYHSLYANVEFSNVHFSYPSRPEQKVLDNFSLSLPRGKVVAICGPSGAGKSTVAALLERFYDLKEGSITIDGHSLEKLDPSWLRGHTIGYINQEPTLFATSVMENIRYGKAGATDVEVMEAAVTAGAHEFISEFPQGYQTVLGERGVTVSGGQKQRIAIARAILKNPSILILDEATSALDAESERLVQASLDRLSKGNSFHGSLDDHFVSLYGLNTFLFILVCSPVKERTFGLHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HIR9</accession>
    <name>A0A9Q1HIR9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_00537</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HIR9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8048284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="67" mass="7655" checksum="F38199981E978F12" modified="2023-09-13" version="1">MFPMEAYLNNLYYDPSHPAAFGGVGAIKRQQNRINETLASKRLQSGCRGEMLILCTNLRKKHFNAIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HJ43</accession>
    <name>A0A9Q1HJ43_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Crystal protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_06687</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HJ43"/>
    <dbReference type="OrthoDB" id="3200163at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019819">
        <property type="entry name" value="Carboxylesterase_B_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45570:SF2">
        <property type="entry name" value="ACETYLCHOLINESTERASE 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45570">
        <property type="entry name" value="CARBOXYLIC ESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00941">
        <property type="entry name" value="CARBOXYLESTERASE_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040159921" evidence="1">
        <location>
            <begin position="21"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="domain" description="Carboxylesterase type B" evidence="2">
        <location>
            <begin position="29"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="domain" description="Carboxylesterase type B" evidence="2">
        <location>
            <begin position="511"/>
            <end position="882"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8047640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="928" mass="104561" checksum="A8C87DBE9D120CAD" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HKQ0</accession>
    <name>A0A9Q1HKQ0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_01213</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HKQ0"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="32"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="158"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="205"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="177"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8048766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="342" mass="38089" checksum="7F15716BEA42DAD6" modified="2023-09-13" version="1">MSTSDLNSPPTSDPPIIITSTVNVPIDMAATQGSQVDGAGGGEEVERRSSSRTKQFTEKGLGYEIEKRERNFRTKVSAWRRRAGRIESMLSESTDSSTLKQERELLGKDMNEVIVANEENNEILVKAEKPIAYSHFEAIESDNYHLLKRISERIKEIEKQSEIASGTRSKSRRSSHSSHSSKGSISKRAEAAAQAAVLKAKLKYIDQEAEQKANLEKLRADLDKLQIMKEIDMAEAKVDAIVKVEQDTNTIFDADKLSHLIEDKEQFVSDYVESHSYVPTTDKIPLSVPVTDPIGMQPFPSFSQPISRETVYSTGLYFSSVHSDPVHGFSSTGENTQKYLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HLC5</accession>
    <name>A0A9Q1HLC5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">RNA-directed DNA polymerase from mobile element jockey</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_03439</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HLC5"/>
    <dbReference type="OrthoDB" id="6084178at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052560">
        <property type="entry name" value="RdDP_mobile_element"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36688">
        <property type="entry name" value="ENDO/EXONUCLEASE/PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36688:SF2">
        <property type="entry name" value="ENDONUCLEASE_EXONUCLEASE_PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14529">
        <property type="entry name" value="Exo_endo_phos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="3">
        <location>
            <begin position="193"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="498"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="127"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8050295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="641" mass="71874" checksum="90F166753A2EA8F1" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HLI6</accession>
    <name>A0A9Q1HLI6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_02641</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HLI6"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018465">
        <property type="entry name" value="Scm3/HJURP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10384">
        <property type="entry name" value="Scm3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="353"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="353"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="378"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8049753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="716" mass="79045" checksum="099C1DD617B22044" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YE64</accession>
    <name>A0A9Q0YE64_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_38352</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YE64"/>
    <dbReference type="OrthoDB" id="10507082at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="CDD" id="cd00821">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="3">
        <location>
            <begin position="234"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="3">
        <location>
            <begin position="501"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="90"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="165"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="393"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="476"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="601"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="688"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="797"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="874"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="726"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="112"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="215"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="434"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="478"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="601"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="697"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="834"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="874"/>
            <end position="888"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8021218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="989" mass="111393" checksum="2EC6FD6ED53F8118" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YF35</accession>
    <name>A0A9Q0YF35_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">60S ribosomal protein L4-A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_45062</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010001751">
        <property type="protein sequence ID" value="KAJ8017491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YF35"/>
    <dbReference type="OrthoDB" id="10259785at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002136">
        <property type="entry name" value="Ribosomal_uL4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023574">
        <property type="entry name" value="Ribosomal_uL4_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045240">
        <property type="entry name" value="Ribosomal_uL4_euk/arch"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19431">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00573">
        <property type="entry name" value="Ribosomal_L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52166">
        <property type="entry name" value="Ribosomal protein L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8017491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="124" mass="14044" checksum="E70F4BF37A5A39C7" modified="2023-09-13" version="1">MYRGHQQAARPLVTVYTDWTMKPQFGAKRQTLQAVFRAEIRTDIVNYVHTNMRNNTRQPYAVNKYAGHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTRGSARWQYRPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YG49</accession>
    <name>A0A9Q0YG49_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_44181</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000692">
        <property type="protein sequence ID" value="KAJ8018046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YG49"/>
    <dbReference type="OrthoDB" id="10067350at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF11">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="195"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8018046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="256" mass="28631" checksum="F080445B25FC32A8" modified="2023-09-13" version="1">MIDSGALCNIIDMDTWTQLKERKIQCKSEKVNKTLYPYGKSDPLDIIGKFSTYVYAGNIKAENVEFFVMNGAGQALLGKETAEKLKLLAIGYNVNKVDGKQTEESELQVQLDDPKKEFPECFNGVGKLKGYRAKLHIDPETKPVAQKARRIPFGLRDKLDKKLDELLSKDIIEEVKGPTTWVTVQSPEGVTYKRNSSHVKKFESGREVTPQVESSEEGSKSEASGGIADDVGIKSSRPVRTHKMPSRFKNFVIDTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YIC7</accession>
    <name>A0A9Q0YIC7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_40878</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8021104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YIC7"/>
    <dbReference type="OrthoDB" id="10042460at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="128"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="200"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="254"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="322"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8021104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="345" mass="40127" checksum="66E75B4219274CD9" modified="2023-09-13" version="1">MILVFAQLLCGFTYVIRFFGVANNFYHFASFLLTYWPLIVSVSLLCLLTSVRQWLHNKDHYDGSSQWWTVLSESSVSRRFQPFAFSTFRPPKSYYFIFFCLVAFVNISMQIVHVILATGYSKHKMVYVYHFFELATSIHSTWFFSIFCYYLFLQRIALEVHFDDLLWFVKTNAGDIVKCRPRLNACFKDYLRLRQLVRPWITLVICSTTFGLAVHIAWNYKALGTDDNGGNYVTVSPLPHFPITNLTAAKKSDFYFLNTLIFVDKLSVLLLALLAVGGSDVNYIWRRYVFTLNVMRSSRHEKFWDKLSKSMKKFNLDTSSDTIIDLLLPVLLLALGILGISNFDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YIE0</accession>
    <name>A0A9Q0YIE0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_42221</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000043">
        <property type="protein sequence ID" value="KAJ8019276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YIE0"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312">
        <property type="entry name" value="NACHT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312:SF2">
        <property type="entry name" value="NUCLEOTIDE-BINDING OLIGOMERIZATION DOMAIN-CONTAINING PROTEIN 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8019276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="493" mass="56427" checksum="CA109FCB89791310" modified="2023-09-13" version="1">MFRNFKVILTTRPSSTPPKLSYATQQVRLTGFDDQAKEKYILKAVVEGNAKEAARIIQRLQQNPVLADICQVPLFFVMFAHMTYEKETSLVLDSVTSFFRYVIACFYEHIQIRAGTVRISMKGASATKHQKLDKVAFESLQGKDQHLVWRRDRLVQLIDEPLYNELVEIGILVEENVVRIVDEPGTSAADLFQRRKNVSFYHKLFCEWYAAHYVVEHIDGLKTISLQRFFANIDPFDLQYVYRIACGLSTVAAGKIIQYLHSLEGGDKFAILCILEQTGQVDKMKDTIRRMCSEGVIISGYDSLLLQRSSMQLLEIAARNEIPIEYVNLYNCLQSVDLSTAAIRTTSTLALTSRIPVKELSVVLSNRDMTEGEAIDILQFSSMCPSIRQLWFYGCVPPRSFNVGSTLSTLKSKDCEVLWQWTAETPSYILNLRSGRWKNNDDGSEPAEEVFERALSKLSEWRTGYTAETYRAEVKEGRENLRKFAEKRKGYLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YIK0</accession>
    <name>A0A9Q0YIK0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_38291</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8023185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YIK0"/>
    <dbReference type="OrthoDB" id="6086925at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8023185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="67" mass="7136" checksum="5FB487D6960D4009" modified="2023-09-13" version="1">MASSKDEYRSEWICVDCDPQLVSARSVGRHGGAYIYPVEATCGLNDAGLPCSKHPAGNELTCAVCTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YJF9</accession>
    <name>A0A9Q0YJF9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Chromodomain-helicase-DNA-binding protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">HOLleu_38565</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">Nanhai2018</strain>
            <tissue evidence="20">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="5857104at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140658">
        <property type="term" value="F:ATP-dependent chromatin remodeler activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="CD1_tandem_CHD3-4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18662">
        <property type="entry name" value="CD2_tandem_CHD3-4_like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PHD2_CHD_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="chromodomain-helicase-DNA-binding protein 3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000001">
        <property type="entry name" value="chromodomain-helicase-DNA-binding protein 3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000015">
        <property type="entry name" value="chromodomain-helicase-DNA-binding protein 9 isoform X1"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012957">
        <property type="entry name" value="CHD_C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009462">
        <property type="entry name" value="CHD_II_SANT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012958">
        <property type="entry name" value="CHD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009463">
        <property type="entry name" value="DUF1087"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45623:SF17">
        <property type="entry name" value="CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45623">
        <property type="entry name" value="CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08074">
        <property type="entry name" value="CHDCT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06461">
        <property type="entry name" value="CHDII_SANT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08073">
        <property type="entry name" value="CHDNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06465">
        <property type="entry name" value="DUF1087"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DUF1086"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="2"/>
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    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 14" id="KW-0863">Zinc-finger</keyword>
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        <location>
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            <end position="384"/>
        </location>
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    <feature type="domain" description="PHD-type" evidence="17">
        <location>
            <begin position="400"/>
            <end position="447"/>
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        <location>
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        </location>
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        <location>
            <begin position="792"/>
            <end position="976"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="19">
        <location>
            <begin position="1108"/>
            <end position="1257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="151"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
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            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="567"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
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            <end position="1363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1385"/>
            <end position="1448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
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            <end position="1891"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="2103"/>
            <end position="2133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="2214"/>
            <end position="2261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="25"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="60"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="181"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="227"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="282"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="299"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
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            <end position="485"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="1339"/>
            <end position="1354"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
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            <end position="1408"/>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
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            <end position="1437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="1438"/>
            <end position="1448"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
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        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
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            <end position="1630"/>
        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
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            <end position="1891"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
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            <end position="2132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="2214"/>
            <end position="2230"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="2247"/>
            <end position="2261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAJ8021387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="2261" mass="254819" checksum="5CE684BFAF4EC7A8" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YL85</accession>
    <name>A0A9Q0YL85_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein 177</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_34554</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YL85"/>
    <dbReference type="OrthoDB" id="110174at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026620">
        <property type="entry name" value="TMEM177"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21824">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 177"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21824:SF4">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 177"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="169"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="197"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8024608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="315" mass="35289" checksum="BDF0992017C2C2E6" modified="2023-09-13" version="1">MAENGKMSFMNKFRVWLRNPRNFVVLGGSSAVFVTLVKACPLLFPSQLYRPVMEAYKSGQKVKLGESQIQEFEDVCRDLEVETKPFHLFVTSRWDLKVKGLQFFPSGVHFGIPVLFVDKPELQNSKFAAKLKVNMDSEEGKAFKDSIQLSQKARKFALAKEVIYSKKHYAAYPLLLAPGAVIGGFVSTFALQVTASIFPLYITGFLVGSVFYVTYRYLLGVLNERHDLKTDDIVAQLGENYAEGGLEFYEKTLQRNKALRKLLGSKGEALYTYYGNDSPGVLWTKGAPITVKRAKFQNIVQNTSAGDSPTSDKTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YLY5</accession>
    <name>A0A9Q0YLY5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Melatonin receptor type 1C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_34921</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YLY5"/>
    <dbReference type="OrthoDB" id="9946013at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004983">
        <property type="term" value="F:neuropeptide Y receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00637">
        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000611">
        <property type="entry name" value="NPY_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24228">
        <property type="entry name" value="B2 BRADYKININ RECEPTOR/ANGIOTENSIN II RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24228:SF72">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01012">
        <property type="entry name" value="NRPEPTIDEYR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="27"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="63"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="98"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="141"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="193"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="245"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="275"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="42"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8024883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="315" mass="35638" checksum="199DD16D2DB231D3" modified="2023-09-13" version="1">METIYASDAYTTSETYARLDDPKEPRILAIPLGIISLLGIPGNILVIFAVCSSRKLKTSTNFLVANLAFSDLVTCLTMPIHIFTILNGRPPLDSVCVLVGGVFSISLGASILNLSLIAVNRAYLITKPRSQYDQLYSRKKIALMLIFSWVYVTLAVSVPPAFNIGALGYEPLYRVCVTVISHTMNFYYNLLKAVVVAPFIFILILCYVYIYFTVQKTTNASLHMCNQNDQLQQQMRKRQIAVTKNLFIIVCVYTICVTPYFILSVQPSTYHLRPWLVTLALFNSCVNPFIYGFNHPQFKEVFRHILLANFKNRNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YLZ8</accession>
    <name>A0A9Q0YLZ8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_36478</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YLZ8"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8023906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="69" mass="7654" checksum="EC03C6210C58381F" modified="2023-09-13" version="1">MLDSLIGHIIGNTANENNLQEVINDYFVSLGNNSDISDDLDIDELSNNEHDIAFNDDSVKEEVDDGDEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YPZ0</accession>
    <name>A0A9Q0YPZ0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Dystrophin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HOLleu_31312</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Nanhai2018</strain>
            <tissue evidence="18">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <location evidence="2">Sarcolemma</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="18853at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016010">
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</entry>
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        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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    </evidence>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YQX4</accession>
    <name>A0A9Q0YQX4_HOLLE</name>
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        <submittedName>
            <fullName evidence="5">Zinc finger CCHC domain-containing protein 3</fullName>
        </submittedName>
    </protein>
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        <name evidence="5" type="ORF">HOLleu_36116</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:activation of innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
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        <property type="entry name" value="Znf_CCHC_sf"/>
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        <property type="entry name" value="GAG-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZINC FINGER CCHC DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
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        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="45"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="4">
        <location>
            <begin position="62"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="4">
        <location>
            <begin position="82"/>
            <end position="96"/>
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    </feature>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="326"/>
            <end position="356"/>
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    </feature>
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        <location>
            <begin position="150"/>
            <end position="169"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="179"/>
            <end position="302"/>
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        <location>
            <begin position="246"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8023626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="403" mass="45192" checksum="4E01A242BFC03304" modified="2023-09-13" version="1">MFKDYPTVFNGIRQYKMKLNSDIPSSVSFGGRSCWVRYRGQPRTCLKCGEAGHEARECGVIRCFNCQSLGHASKECEVPTTCTICGKSGHHYRDCPVSFANKIKPNSSWIIGSHAKTSEGGEKEAEATLGDEAVEVSSDDAMHLAVAANPGPSFIADNQPPNEVVPDNQTNDSQILLFEDSEPEVIPDTQTSSDVLTEKNPPESTMDTSDEVIPDTQASSDVLTDKKSPESNMDTSDSGPEIKTLENCTPQPQLQDNEKCSNEIQSQPAISVDNDPIFVKPRRKTSSQQTTGRRPERSRSGIRFPIENPMDVIKMTQIFLEDEPWHSCYVKTCCETFSKFKDLKAHVGEHHSKLKPQEYSSARRSCHLTFSTPWEWIQHLARKHAEFVSGKEIEFFDKYFLRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YRB1</accession>
    <name>A0A9Q0YRB1_HOLLE</name>
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        <submittedName>
            <fullName evidence="12">Melanopsin-A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_35357</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8025214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
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        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
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        <property type="entry name" value="B2 BRADYKININ RECEPTOR/ANGIOTENSIN II RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24228:SF72">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
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        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="39"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="75"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="114"/>
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="155"/>
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="204"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="473"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="499"/>
            <end position="520"/>
        </location>
    </feature>
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        <location>
            <begin position="54"/>
            <end position="518"/>
        </location>
    </feature>
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            <begin position="313"/>
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        </location>
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        <location>
            <begin position="361"/>
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        </location>
    </feature>
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        <location>
            <begin position="321"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="361"/>
            <end position="373"/>
        </location>
    </feature>
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        <location>
            <begin position="401"/>
            <end position="426"/>
        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAJ8025214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="553" mass="62078" checksum="7F08860DD5C366E4" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YRK4</accession>
    <name>A0A9Q0YRK4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Splicing factor, proline- and glutamine-rich</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_32283</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="10067824at2759"/>
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        <property type="component" value="Chromosome 16"/>
    </dbReference>
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        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="entry name" value="eNOPS_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RRM1_p54nrb_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12333">
        <property type="entry name" value="RRM2_p54nrb_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="paraspeckle component 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012975">
        <property type="entry name" value="NOPS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
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        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23189">
        <property type="entry name" value="RNA RECOGNITION MOTIF-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08075">
        <property type="entry name" value="NOPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="236"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="310"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="496"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="605"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="21"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="60"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="90"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="128"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="170"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="496"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="655"/>
            <end position="671"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8027208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="682" mass="75247" checksum="7EAF2AC1DFAB8212" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YSM5</accession>
    <name>A0A9Q0YSM5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Endonuclease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_32281</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000032">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 297-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon gypsy-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000020">
        <property type="entry name" value="Transposon Tf2-6 polyprotein-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054465">
        <property type="entry name" value="Integrase_p58-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22938">
        <property type="entry name" value="Integrase_p58_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="4">
        <location>
            <begin position="859"/>
            <end position="1017"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase" evidence="3">
        <location>
            <begin position="1273"/>
            <end position="1450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="328"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="592"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="692"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="78"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="328"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="343"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="367"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="592"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="636"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="661"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="708"/>
            <end position="732"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8027206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1527" mass="174684" checksum="E7942BDEE619AF44" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YTA1</accession>
    <name>A0A9Q0YTA1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_34224</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8026397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YTA1"/>
    <dbReference type="OrthoDB" id="9905391at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21301">
        <property type="entry name" value="REVERSE TRANSCRIPTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21301:SF10">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8026397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="136" mass="15015" checksum="A1B61C1A2FB209D1" modified="2023-09-13" version="1">MYNFALPINTKPARFYLLPKIHKAGVPGRPVISCCGSPTEGISELVDWFIQPLVSSIPSYVKDTNDFLVKIRALGPLAPNSLLVTVDVSALYPSIPHKDGIRNLSLAYYLDKSHFPRESVNAICKLTESLLSKKCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YU61</accession>
    <name>A0A9Q0YU61_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Unconventional myosin-VI</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_31283</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YU61"/>
    <dbReference type="OrthoDB" id="6108017at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030139">
        <property type="term" value="C:endocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030048">
        <property type="term" value="P:actin filament-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140">
        <property type="entry name" value="MYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140:SF745">
        <property type="entry name" value="UNCONVENTIONAL MYOSIN-VI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="2 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 6" id="KW-0505">Motor protein</keyword>
    <keyword evidence="3 6" id="KW-0518">Myosin</keyword>
    <keyword evidence="1 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myosin motor" evidence="7">
        <location>
            <begin position="1"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="14"/>
            <end position="21"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8026461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="219" mass="25065" checksum="5CF82392C0E36596" modified="2023-09-13" version="1">MVASKQSQSVIVSGESGAGKTETTKYILRYLTESHGQSAGIIEQRIIEANPLLEAFGNAKTVRNNNSSRFGKFVEMHFNAKPQVVGGHVSHYLLEKPRVCWQSKAERNYHIFYYLCAGAPEKTRKDLKITQPDNYQYMKHGNTQYFTNKETEKKVPQDGKSYQVGKKKHQGVEVKKGIIMSYDCTFKSQKQTFFTIRWATTRWIPSGLITLTIGYFIVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BA22</accession>
    <name>A0A9Q1BA22_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_39738</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BA22"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8020211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="108" mass="12732" checksum="56C5F60ED9225E9F" modified="2023-09-13" version="1">MSTGTKPWAWVQVWEPGHEYRWGPVQVRVTGHEYVVLSLGMVYMPGREKLVWVQVPGYELEHRLGVTQHEYGHRHDSRTFIPKCRFQVAPPSPHLWAPCPLRQSWHNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BC46</accession>
    <name>A0A9Q1BC46_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_37130</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BC46"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8022281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="139" mass="16102" checksum="3B0884DF23FB61E9" modified="2023-09-13" version="1">MESSTFSNGIQESPWEFHRHHAFPTGSFKITMGNATFPMNCPREMEISRGKFSFSLRLKLTNPHESRLISHGKCYVPWEVVPFHREVTSPHGNCNISWEMSLLHGMCQSPMVFVISPYLNHVQYHIIPHKLCMCLIPFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BFH8</accession>
    <name>A0A9Q1BFH8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nucleotide exchange factor SIL1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_33523</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Required for protein translocation and folding in the endoplasmic reticulum (ER). Functions as a nucleotide exchange factor for the ER lumenal chaperone HSPA5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SIL1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BFH8"/>
    <dbReference type="OrthoDB" id="448649at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000774">
        <property type="term" value="F:adenyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000148">
        <property type="entry name" value="SIL1 nucleotide exchange factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050693">
        <property type="entry name" value="Hsp70_NEF-Inhibitors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013918">
        <property type="entry name" value="Nucleotide_exch_fac_Fes1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19316:SF35">
        <property type="entry name" value="NUCLEOTIDE EXCHANGE FACTOR SIL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19316">
        <property type="entry name" value="PROTEIN FOLDING REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08609">
        <property type="entry name" value="Fes1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0811">Translocation</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040503368" description="Nucleotide exchange factor SIL1" evidence="11">
        <location>
            <begin position="29"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleotide exchange factor Fes1" evidence="12">
        <location>
            <begin position="154"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF08609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8025851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="470" mass="53450" checksum="A6EB1886E9726EF8" modified="2023-09-13" version="1">MSMKTKLKMFHVVTFLLFLISCYWSVLGKDSTDVILVESSPDEEDGTITTEDGEEQIVDVFEPTKEWKVIKPGQAVPRGLHIRIDLSTGLKEAKLMDENEELEEENVDEKNEDSESTMRYWKDGTKEGIRHSKQKYFTHDELKNAMKLFKANQDDVKDKEAAKKIREKYRSIEEIKEDFEAMELDLETDVEVMTKLIGRYNRSGATVEEKIAALQDLEYYVHQIDNAKDLATIGGLQLIIAGLNDSDDAIRQECAFVLGSAVQSNPHVQVQAVDSGALQLLLQVLSTSQSVPLRKKAMYAISGLLRMFPYAQMKFLELGGLSTFSSLFKGVDPSVVTLKVKAVTLLHDLLVEKQDVFQYDDEGDSVHQLRKEQYKRVDLLPKMLEQNWCQLIPSLLEVPDSDVQEKVLQAMEFLMDPCHHFSQDTEVVQILLKLKRSLQSQMESQASDPDGSYVYDLTVLVDNIMNHLSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BFJ9</accession>
    <name>A0A9Q1BFJ9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Netrin receptor UNC5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_36259</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Receptor for netrin required for axon guidance. Mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the unc-5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="7457896at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005042">
        <property type="term" value="F:netrin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.220.30">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037936">
        <property type="entry name" value="UNC5A-D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000906">
        <property type="entry name" value="ZU5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12582:SF47">
        <property type="entry name" value="NETRIN RECEPTOR UNC-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12582">
        <property type="entry name" value="NETRIN RECEPTOR UNC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00791">
        <property type="entry name" value="ZU5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00218">
        <property type="entry name" value="ZU5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51145">
        <property type="entry name" value="ZU5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="1" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="1 4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ZU5" evidence="3">
        <location>
            <begin position="1106"/>
            <end position="1237"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="112"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU367033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8023733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1512" mass="172423" checksum="8D5813B2A27FDE34" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BFV5</accession>
    <name>A0A9Q1BFV5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Apoptosis regulator BAX</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_36156</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Bcl-2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BFV5"/>
    <dbReference type="OrthoDB" id="6080198at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051400">
        <property type="term" value="F:BH domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015267">
        <property type="term" value="F:channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097192">
        <property type="term" value="P:extrinsic apoptotic signaling pathway in absence of ligand"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008630">
        <property type="term" value="P:intrinsic apoptotic signaling pathway in response to DNA damage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008053">
        <property type="term" value="P:mitochondrial fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001836">
        <property type="term" value="P:release of cytochrome c from mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06845">
        <property type="entry name" value="Bcl-2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.437.10">
        <property type="entry name" value="Blc2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036834">
        <property type="entry name" value="Bcl-2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046371">
        <property type="entry name" value="Bcl-2_BH1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026298">
        <property type="entry name" value="Bcl-2_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002475">
        <property type="entry name" value="Bcl2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11256:SF56">
        <property type="entry name" value="BCL-2 BCL-2 HOMOLOGY REGION 1-3 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11256">
        <property type="entry name" value="BCL-2 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00452">
        <property type="entry name" value="Bcl-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00337">
        <property type="entry name" value="BCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56854">
        <property type="entry name" value="Bcl-2 inhibitors of programmed cell death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50062">
        <property type="entry name" value="BCL2_FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="147"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="184"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="Bcl-2 Bcl-2 homology region 1-3" evidence="5">
        <location>
            <begin position="74"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8023659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="207" mass="23799" checksum="10D76F2EC0069631" modified="2023-09-13" version="1">MATGGGGSRDEDNQYQRQLSEEDIGQQATVLLQRFIVERFEQDCVENAPTLAEIRGTEEESEHDRVWIEVGSLIREIGDALDRDQELQRMIDSVPEESTIDNIIAVAHVIFRDGEINWGRVIGLFYFAYQMCARAMSRFMDENFPEWVNRLISAVVQFLVLKFADWIINRGGWLAIREYFGSPTWIWGTLVCVSALCLLIGIYKLQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BGE6</accession>
    <name>A0A9Q1BGE6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_34669</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BGE6"/>
    <dbReference type="OrthoDB" id="6502630at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8024693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="88" mass="9862" checksum="6F4D15699CD06E86" modified="2023-09-13" version="1">MRYGMRKNPIIFGGGERPSGVTGGQTLKTLLTRYLKMRYGMRKNPIIFGGGERSSGVTRGHTLKTLLTRYLKKGSSDSSQTWYIDAPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BGN0</accession>
    <name>A0A9Q1BGN0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_36486</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BGN0"/>
    <dbReference type="OrthoDB" id="8939918at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33332">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8023912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="251" mass="28845" checksum="EE33C7CCCD63B057" modified="2023-09-13" version="1">MTHNILKLNADKPEVLVIGFRAQLLQFNLPSINIAGVNVAVRSDPIKNLRVMFDPRMSMNAQVTNIVKAANSHLINISRARRFLITEAAKLAIHALVTSRLDYCNGILIGISNRVLTQLQNIQRTSARLITNRRKFDSITSDLIQLHWLPVKQRIDFKILLLVYKALHNQTPGEITNMLQVNIRQRRLRSSCSSSHPLVEPHTRCVSFADRSFSVYAPKIWNKLPEYKKVISYEIFKSLLKAHLFHEVYQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BHU7</accession>
    <name>A0A9Q1BHU7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Leucine-rich repeat-containing protein 15</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_32340</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BHU7"/>
    <dbReference type="OrthoDB" id="676979at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366">
        <property type="entry name" value="IG(IMMUNOGLOBULIN) AND LRR(LEUCINE RICH REPEAT) DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="451"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="480"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="484"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8027245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="510" mass="57159" checksum="B44FD2DC23388F44" modified="2023-09-13" version="1">MQKHIAHTLLKTPVFRRNGKAKMGVLLSGEYIVGSCILKTFLLLTSTTILVVSIPTNVTGDQVQPTDSELTLTCGNICEYEEWFSRAHCDERGLTYVPRCTGCEGATLLELQANNIQSIPPERIAEYYHVKTMDISWNQISVLDPGTFFQLNHLKNIICSHNGLHIVQNGLFNGTEHKLSRIYLNNNNIHIIGDEAFKKLHQLIAIYLNNNHIQSLPQNIFSDVTNIKYLILSDNDLHYVEIDQFKGLTKLEHLSLDANNLKIIPKGLFTGLESLKVVLLSHNKLITIPAPHHLGLHLGLERLDLRNNHLNQSAMTISYIVNATWVVNLNDNPFLCDCFFQSIQDLFKTTPDSQASMTCTLTNGTVYSISDYLPISCSDMSKENNTSTVTNYTTVLPFTPHNKGKDSVVTLDLYSNVSFTTLQNNKNVVQDQERQVTTVMTSCDGITTATLIVLVIFFALWLGKSIWCTVLYIRRQKGKEGNGVSPQNVPGGNSHNDGCREENQPLNIEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BIK2</accession>
    <name>A0A9Q1BIK2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="5">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HOLleu_32355</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Nanhai2018</strain>
            <tissue evidence="18">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase C64 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="5"/>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BIK2"/>
    <dbReference type="OrthoDB" id="10064699at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070530">
        <property type="term" value="F:K63-linked polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030177">
        <property type="term" value="P:positive regulation of Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071947">
        <property type="term" value="P:protein deubiquitination involved in ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035523">
        <property type="term" value="P:protein K29-linked deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990168">
        <property type="term" value="P:protein K33-linked deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051346">
        <property type="entry name" value="OTU_Deubiquitinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003323">
        <property type="entry name" value="OTU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002653">
        <property type="entry name" value="Znf_A20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13367">
        <property type="entry name" value="UBIQUITIN THIOESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13367:SF28">
        <property type="entry name" value="UBIQUITIN THIOESTERASE ZRANB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02338">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01754">
        <property type="entry name" value="zf-A20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00259">
        <property type="entry name" value="ZnF_A20"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50802">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="11" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="14" id="KW-0862">Zinc</keyword>
    <keyword evidence="10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="OTU" evidence="17">
        <location>
            <begin position="99"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="478"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="550"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="624"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="679"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="752"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="913"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="478"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="553"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="679"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="775"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="934"/>
            <end position="947"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ8027257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1042" mass="117901" checksum="51919392E86CE9C6" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BIQ4</accession>
    <name>A0A9Q1BIQ4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_32454</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BIQ4"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040363097" evidence="2">
        <location>
            <begin position="20"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="131"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8027338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="170" mass="18872" checksum="4E72E9BA1F19AEDF" modified="2023-09-13" version="1">MFFSVLLMTLLTSGYLSEAKRITVKDFPPKIPKLKPENMRQPQPFHCTASYRENKDDKVITASSWTNDGQTGVSKSPNQNMPRQENAISHTKTDKTGSNMTPFSMVTTDDHSEPSKSTNKGEVKQDEVRSSFLSTAESNNVASDSTSQNVTTDESTVNYNSPTNSGRFCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BJA4</accession>
    <name>A0A9Q1BJA4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">catechol O-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_32819</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-dependent O-methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.6" evidence="1"/>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BJA4"/>
    <dbReference type="OrthoDB" id="186626at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016206">
        <property type="term" value="F:catechol O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042424">
        <property type="term" value="P:catecholamine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032502">
        <property type="term" value="P:developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042417">
        <property type="term" value="P:dopamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000054">
        <property type="entry name" value="Catechol O-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002935">
        <property type="entry name" value="SAM_O-MeTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43836">
        <property type="entry name" value="CATECHOL O-METHYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43836:SF2">
        <property type="entry name" value="CATECHOL O-METHYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01596">
        <property type="entry name" value="Methyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51682">
        <property type="entry name" value="SAM_OMT_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0128">Catecholamine metabolism</keyword>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-0531">Neurotransmitter degradation</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8027621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="251" mass="28624" checksum="3CF745ACB1DA4B8A" modified="2023-09-13" version="1">MTSDFIPHRIPLPLYKSAIIWYAAQYRKLRNIVYWSTEPDRLLTFVKKIADEGDVPGILAACDKYCVEKEWIMHLGGEKGKIFCEIIRDCQPRLCLELGTYCGFSAILLASLLPDDGKVYTVEFDENYAKAAKALVKYAKLDHKITILCGDAGEVIPTLRDRYSLDKFDFVFIDHHKPLYCRDLKLLVSNDHLRKGSVVVADDLRWPGAPDYDEYVQKSKDFKSTFFDTKTVFLDILDVMGKSVFTGGDPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BKP8</accession>
    <name>A0A9Q1BKP8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Calpain-15</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HOLleu_30691</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Nanhai2018</strain>
            <tissue evidence="16">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BKP8"/>
    <dbReference type="OrthoDB" id="424753at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004198">
        <property type="term" value="F:calcium-dependent cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00044">
        <property type="entry name" value="CysPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000010">
        <property type="entry name" value="Calpain 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1060.10">
        <property type="entry name" value="Zinc finger, RanBP2-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.380">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022684">
        <property type="entry name" value="Calpain_cysteine_protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001300">
        <property type="entry name" value="Peptidase_C2_calpain_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036443">
        <property type="entry name" value="Znf_RanBP2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10183">
        <property type="entry name" value="CALPAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10183:SF382">
        <property type="entry name" value="CALPAIN-15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00648">
        <property type="entry name" value="Peptidase_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00641">
        <property type="entry name" value="Zn_ribbon_RanBP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00704">
        <property type="entry name" value="CALPAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00230">
        <property type="entry name" value="CysPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00547">
        <property type="entry name" value="ZnF_RBZ"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90209">
        <property type="entry name" value="Ran binding protein zinc finger-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50203">
        <property type="entry name" value="CALPAIN_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01358">
        <property type="entry name" value="ZF_RANBP2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50199">
        <property type="entry name" value="ZF_RANBP2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="3 11" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 11" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RanBP2-type" evidence="14">
        <location>
            <begin position="5"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="RanBP2-type" evidence="14">
        <location>
            <begin position="97"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="RanBP2-type" evidence="14">
        <location>
            <begin position="209"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="RanBP2-type" evidence="14">
        <location>
            <begin position="243"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="Calpain catalytic" evidence="15">
        <location>
            <begin position="325"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="70"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="276"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="71"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="active site" evidence="10 11">
        <location>
            <position position="392"/>
        </location>
    </feature>
    <feature type="active site" evidence="10 11">
        <location>
            <position position="555"/>
        </location>
    </feature>
    <feature type="active site" evidence="10 11">
        <location>
            <position position="575"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR622684-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ8028458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="904" mass="100505" checksum="E42520105B5AE4F2" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BLN2</accession>
    <name>A0A9Q1BLN2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_31090</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BLN2"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8028759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="56" mass="5866" checksum="56B0D33159A00BDE" modified="2023-09-13" version="1">MLFSMEKATLDDADTSSPSSSKGEKSWYDEKGSSAGPFGCTRITCEGAFMSASATC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BMH2</accession>
    <name>A0A9Q1BMH2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Sorbitol dehydrogenase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Polyol dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_28724</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BMH2"/>
    <dbReference type="OrthoDB" id="1879366at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003939">
        <property type="term" value="F:L-iditol 2-dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006062">
        <property type="term" value="P:sorbitol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05285">
        <property type="entry name" value="sorbitol_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000068">
        <property type="entry name" value="Sorbitol dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045306">
        <property type="entry name" value="SDH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43161">
        <property type="entry name" value="SORBITOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43161:SF9">
        <property type="entry name" value="SORBITOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alcohol dehydrogenase-like N-terminal" evidence="11">
        <location>
            <begin position="29"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="Alcohol dehydrogenase-like C-terminal" evidence="10">
        <location>
            <begin position="181"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00026132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8029351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="352" mass="38039" checksum="EDDA3BD8345539D9" modified="2023-09-13" version="1">MAPTDRNLSVVTTGKDDLLLEDRDIPLPGKDEVQIAMQRVGICGSDVHFKKHGGIGDYKVKSPIILGHESSGIVSAVGTNVKHLKVGDRVAIEPGIPCRKCKFCKEGRYNLCPNCFFLACPPDDGSLRRYHCHAADFCFKLPDNVSSDEGALMEPLSVAIHACRRAGLTLGDKVLVLGAGPIGLVCLLTSKAMGAAKVAITDIQDDRLKVANELGASACIKIDESDPKKMAEKVHTTLGWKPDITIECSGVESSFQMGIYATETGGVFQMVGHGNPIKSFPQFVANTREIDIRGTQRYANTYPTALSMIANGLVDVKPLITHRFPLKESVEAFNAAETCKDNAIKVMIHCDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BNQ3</accession>
    <name>A0A9Q1BNQ3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Protein misato-like 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_29621</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the misato family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8030047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BNQ3"/>
    <dbReference type="OrthoDB" id="271881at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06060">
        <property type="entry name" value="misato"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049942">
        <property type="entry name" value="DML1/Misato"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029209">
        <property type="entry name" value="DML1/Misato_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019605">
        <property type="entry name" value="Misato_II_tubulin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13391">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION REGULATOR MISATO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13391:SF0">
        <property type="entry name" value="PROTEIN MISATO HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10644">
        <property type="entry name" value="Misat_Tub_SegII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14881">
        <property type="entry name" value="Tubulin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Misato Segment II tubulin-like" evidence="5">
        <location>
            <begin position="3"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="DML1/Misato tubulin" evidence="6">
        <location>
            <begin position="161"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="118"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="121"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF10644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF14881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8030047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="577" mass="63870" checksum="BC05374F50E93F68" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BP01</accession>
    <name>A0A9Q1BP01_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_29480</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the mTERF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BP01"/>
    <dbReference type="OrthoDB" id="637682at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006393">
        <property type="term" value="P:termination of mitochondrial transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.70.10">
        <property type="entry name" value="Transcription termination factor 3, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003690">
        <property type="entry name" value="MTERF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038538">
        <property type="entry name" value="MTERF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15437">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15437:SF6">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02536">
        <property type="entry name" value="mTERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00733">
        <property type="entry name" value="Mterf"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8029944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="240" mass="28326" checksum="1DB5D48BDB63A3A6" modified="2023-09-13" version="1">MQRVPRFFMSSPSVVQDIVNYMVNVGVSNKNIRNLIIFSPSLFYRVKGRPQQIGNYLLTVIQKQQPNADVISILPHMLRNDVKLFSRTVNEVSRNLKFLSELGFEGENLVKIIRYCPSALRIGTDFLQRRWDYLKERLVLNDKEVILFVTKYPRILTLADEKIEDIFDFLFEKAGFQLQNIVKNPRLFERSRPHLEERLQILLEVKYKGDFIPMLTVWGKVFDQRIAKLKQMSDIKDRTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BQD2</accession>
    <name>A0A9Q1BQD2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein CREG1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_27119</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BQD2"/>
    <dbReference type="OrthoDB" id="46836at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055343">
        <property type="entry name" value="CREG_beta-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13343:SF17">
        <property type="entry name" value="CELLULAR REPRESSOR OF E1A-STIMULATED GENES, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13343">
        <property type="entry name" value="CREG1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13883">
        <property type="entry name" value="CREG_beta-barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040146376" evidence="1">
        <location>
            <begin position="23"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="CREG-like beta-barrel" evidence="2">
        <location>
            <begin position="31"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8030649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="203" mass="22935" checksum="AD455A0824AD0250" modified="2023-09-13" version="1">MHCNLAFSLLIAVLVLLNIVLGEPVLGTDRPPYQEYAKRARYIVNRANWTVVATFSFHDPVTTFPYARVESCSDGLLHNGTGTPYLYLMKDGTPMQNLKKNGNATLSYSEAEFGDIQSCMISPKSDPENPLCSRVMLYGQFYQVTDKKELQIAQKALFTRHPFMKDLPASHQFQVYGFKISHIGILDYFGGAVWVDVDDYYNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BQZ5</accession>
    <name>A0A9Q1BQZ5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_27939</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8031262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BQZ5"/>
    <dbReference type="OrthoDB" id="5985715at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48251">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48251:SF1">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="125"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="242"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8031262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="316" mass="37411" checksum="3A3266E49FF6769E" modified="2023-09-13" version="1">MDKSHWTQDLFPPTFHLTRAEVDSSAKTKKETLDTSPQTRIQAIYKKVKKEYDEEVWNARQKGTQVGDSLKDFREAKKTVHKINCLEKGCDKSCQENLSIADSELWDSREIEILREVFLKSRTENMKLKIELQSVKEEIKKVSEILKCKEEDYNETDEKFKKSQRAFHNLQLENKLLKKEMKVLDRRNKSLQEELLAIKDTNNKLRKEKTHERRHFLKEQTLRRDVEVALNKVLIQTRTKDRAEVETLKINYELQLKKLNESLTEAKEELEKEKEDHEQTRKALDHLRYHFASLPSKPNITTSSPNDDILSSLQIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BS33</accession>
    <name>A0A9Q1BS33_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_25160</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BS33"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8031832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="88" mass="9900" checksum="DED20336C41376FD" modified="2023-09-13" version="1">MRCGERKNPIVFGGGQRSSGVTGGQNLKTLFTRYLKMRCGERKNPIVFGGGQRSSGVTGGQNLKTLFTRYLKLESLDRFHAWYVDALW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BWM5</accession>
    <name>A0A9Q1BWM5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_20828</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BWM5"/>
    <dbReference type="OrthoDB" id="7920740at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8034120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="60" mass="6650" checksum="8EA5543833EBE839" modified="2023-09-13" version="1">MRLQKLYDVIIPPDDNGEGEGAAPNVQKNADAFAELVQCLDDRSLSLVMQRMTAEGHYKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BWR2</accession>
    <name>A0A9Q1BWR2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Apple domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_21025</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BWR2"/>
    <dbReference type="OrthoDB" id="5916958at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01099">
        <property type="entry name" value="PAN_AP_HGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.4.10">
        <property type="entry name" value="Hepatocyte Growth Factor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052774">
        <property type="entry name" value="Celegans_DevNeuronal_Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47327">
        <property type="entry name" value="FI18240P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47327:SF1">
        <property type="entry name" value="RE15579P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00024">
        <property type="entry name" value="PAN_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00473">
        <property type="entry name" value="PAN_AP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57414">
        <property type="entry name" value="Hairpin loop containing domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50948">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040310884" description="Apple domain-containing protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="2">
        <location>
            <begin position="19"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8034262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="365" mass="41911" checksum="CEB292713ECCFB66" modified="2023-09-13" version="1">MKTAVLTTIFFITVLRQSCVAQPEGEWTDIVSACIRGNDNWSGSASTMEECQELCESREDFRCLSVEFLPSTRTCLHSRYNRNSVPSTDDFTQPCYITGYQYSERRDFNFPWTETVEACIRLNNHGEHTVSSMEECQLLCERETDFQCLSVDFRDDRCLLSRYNRESVRPLSDYTQPCYVDGYQYAERVDKKYFWTDTAFACIRGNNNERHQNIESLEDCMDLCIEETSFTCLSVDFFPDSDNKDCLLSSFNRNSVQPSSDFTQPCYTRPTDGLYAERVNVGQWIPVIEDSCISGFNEWSGDVEDFESCKILCSQRSNCCSVDYNTGTNRCLMTSETRATASNTSFVSPCNGFSYAERTDVRCVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BZC3</accession>
    <name>A0A9Q1BZC3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Leucine-rich repeat-containing protein 15</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_19274</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8035560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BZC3"/>
    <dbReference type="OrthoDB" id="4691307at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000372">
        <property type="entry name" value="LRRNT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050333">
        <property type="entry name" value="SLRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45712">
        <property type="entry name" value="AGAP008170-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45712:SF22">
        <property type="entry name" value="INSULIN-LIKE GROWTH FACTOR-BINDING PROTEIN COMPLEX ACID LABILE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00013">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="696"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="782"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="809"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="849"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="905"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="974"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="1016"/>
            <end position="1037"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="1043"/>
            <end position="1063"/>
        </location>
    </feature>
    <feature type="domain" description="LRRNT" evidence="6">
        <location>
            <begin position="65"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8035560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1128" mass="127593" checksum="E99932B1C483DDEC" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BZV7</accession>
    <name>A0A9Q1BZV7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_20477</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BZV7"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040315257" evidence="1">
        <location>
            <begin position="22"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8036483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="143" mass="16337" checksum="F79F42E51DA8656C" modified="2023-09-13" version="1">MTDKTTFFRFCLLCCLQLLYCCQVETKLHHLKEKGKVKTGECLMENCGPPFYYEIIPNPPSLEKQFTILYGLKLVADVYKAFLHWSVEGPSGIHFGSTTPFCVEHERAEELCPHVAGYYVHGNYTDHVPIFIKPGLLVETVFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C2R1</accession>
    <name>A0A9Q1C2R1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Heparan sulfate 2-O-sulfotransferase hst-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_18101</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sulfotransferase 3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C2R1"/>
    <dbReference type="OrthoDB" id="10019582at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007734">
        <property type="entry name" value="Heparan_SO4_2-O-STrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005331">
        <property type="entry name" value="Sulfotransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12129">
        <property type="entry name" value="HEPARAN SULFATE 2-O-SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12129:SF15">
        <property type="entry name" value="URONYL 2-SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03567">
        <property type="entry name" value="Sulfotransfer_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8037312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="333" mass="38249" checksum="607510C66E2DE436" modified="2023-09-13" version="1">MKTCAGARPKPFFLLVLAICGFLVMGVILESVTDVQLFCRGSTFLTTPFNGRYRLKHDLAHSSFGRPFFGVNYTIIFNAVPKTGSRTLETAVKNLSTAKRVGKPMRCVPIPAETWQSKQNFEDFLSLKKPPLFMRGHASFPAIDRPDLLYIGIVREPVDRVISQFNFMLHGDASTNYQPVMEPERKKILDDLGVTTIDDCYYSSSTVCQSFLDNLRSTTIRIFCGMHPDCRTPSRWSLRKAIENVDRYTVVGLQSYFSDMVKILETLGPDAFGGLYEAYEFVKRNTTKKFSTSKKIVPSNRTFMLLKKDMKLEYEFYHYVEEKFFKLKKRLKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C2T8</accession>
    <name>A0A9Q1C2T8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">TNF receptor-associated factor 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_17879</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C2T8"/>
    <dbReference type="OrthoDB" id="10051587at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031663">
        <property type="term" value="P:lipopolysaccharide-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043122">
        <property type="term" value="P:regulation of canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000121">
        <property type="entry name" value="TNF receptor-associated factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000179">
        <property type="entry name" value="TNF receptor-associated factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.210.10">
        <property type="entry name" value="Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002083">
        <property type="entry name" value="MATH/TRAF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012227">
        <property type="entry name" value="TNF_rcpt-assoc_TRAF_met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008974">
        <property type="entry name" value="TRAF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049342">
        <property type="entry name" value="TRAF1-6_MATH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001293">
        <property type="entry name" value="Znf_TRAF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10131">
        <property type="entry name" value="TNF RECEPTOR ASSOCIATED FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10131:SF152">
        <property type="entry name" value="TNF RECEPTOR-ASSOCIATED FACTOR 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21355">
        <property type="entry name" value="TRAF-mep_MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02176">
        <property type="entry name" value="zf-TRAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015614">
        <property type="entry name" value="TRAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50144">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50145">
        <property type="entry name" value="ZF_TRAF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="10">
        <location>
            <begin position="48"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="TRAF-type" evidence="12">
        <location>
            <begin position="133"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="TRAF-type" evidence="12">
        <location>
            <begin position="186"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="195"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="MATH" evidence="11">
        <location>
            <begin position="321"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="TRAF-type" evidence="7">
        <location>
            <begin position="133"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="TRAF-type" evidence="7">
        <location>
            <begin position="186"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="270"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ8037134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="470" mass="53865" checksum="D8773F75ECAFF45D" modified="2023-09-13" version="1">MPPSRNSSTSSLRSDRTSSSSTSSLPPVEEKYGFEAHFDPVLDKKYVCPVCLCGLREPVQTKCGHRFCRACILKATGTQSIAKCPIDKTIFNIKTELFEDLAVKREVLSHTVLCLNKDRGCSWKAELRSLEEHAEQCEFTLVKCNNGCGEEFILQDEPLHEGECPLRVVTCSYCKESVVHRDLTQHFQACPRYPVSCNQCGKTELLREELAKHIDHYDGDCPEVIVPCSFEEMGCRDEIKRKLMSIHLQEATNQHFHLMYSYTCDLKRLLRNQEAMLRETQDTCNQLICEQADLARQLVEKNNLLSRQADRLKEISTTSHTGLLHWKVALPSQNYTSPLLSPPFYTSRPGYKFRLCLELRGHVARSETYASLFVMLCRGEFDDQLFYPFNGVCHVTVFNQSNPRPSSSDQQHLVTTIECQNMSRAETDNCQNQKRGRLRFIKTESLTKGKFCHNRELFLKAEIVLDHARS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C2V4</accession>
    <name>A0A9Q1C2V4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 10">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="2 10">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HOLleu_17924</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Nanhai2018</strain>
            <tissue evidence="15">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 17 10">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="2 10"/>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C2V4"/>
    <dbReference type="OrthoDB" id="1902587at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052273">
        <property type="entry name" value="PPIase_FKBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46222">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FKBP7/14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46222:SF3">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 10 15" id="KW-0413">Isomerase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 10" id="KW-0697">Rotamase</keyword>
    <keyword evidence="3 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040106454" description="peptidylprolyl isomerase" evidence="12">
        <location>
            <begin position="22"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase FKBP-type" evidence="13">
        <location>
            <begin position="56"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="14">
        <location>
            <begin position="162"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="213"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="225"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ8037175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="235" mass="27304" checksum="7DFA85B11E969FAF" modified="2023-09-13" version="1">MNWVSTVTVCLLVFMMECGHCGKIYEAKRDPRVKYEILKESPNCRAKLAKTYQGWMDFVGRYQDTNETFINSTTLGQEVRKRNEEVGDTIGFPMDYDYLFLGLEIGLEGQCRHETRRIWIPKELVTGGQKIFSPEYIPRGRDLVFDIVLWNVAANYMIGMPNMFKVYDKDKDGYITKKEAGEYITETEKYPSGPLVTKLVNEFIERDDMDGDKRVSFDEFSGPKYDSDGTRHEEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C3G7</accession>
    <name>A0A9Q1C3G7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Eukaryotic peptide chain release factor GTP-binding subunit ERF3A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_18083</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C3G7"/>
    <dbReference type="OrthoDB" id="342024at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003747">
        <property type="term" value="F:translation release factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01883">
        <property type="entry name" value="EF1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03704">
        <property type="entry name" value="eRF3_C_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04089">
        <property type="entry name" value="eRF3_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000017">
        <property type="entry name" value="Eukaryotic peptide chain release factor GTP-binding subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000024">
        <property type="entry name" value="Eukaryotic peptide chain release factor GTP-binding subunit ERF3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000270">
        <property type="entry name" value="Eukaryotic peptide chain release factor GTP-binding subunit ERF3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054696">
        <property type="entry name" value="GTP-eEF1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009818">
        <property type="entry name" value="PAM2_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050100">
        <property type="entry name" value="TRAFAC_GTPase_members"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23115">
        <property type="entry name" value="TRANSLATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22594">
        <property type="entry name" value="GTP-eEF1A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07145">
        <property type="entry name" value="PAM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0866">Nonsense-mediated mRNA decay</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="9">
        <location>
            <begin position="144"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="20"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="75"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="85"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="109"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="132"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8037296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="568" mass="62614" checksum="5DE92514F1E9FACD" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C3L5</accession>
    <name>A0A9Q1C3L5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Tyrosine--tRNA ligase</fullName>
            <ecNumber evidence="15">6.1.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Tyrosyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HOLleu_18409</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Nanhai2018</strain>
            <tissue evidence="17">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 15">
            <text>tRNA(Tyr) + L-tyrosine + ATP = L-tyrosyl-tRNA(Tyr) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10220"/>
            <dbReference type="Rhea" id="RHEA-COMP:9706"/>
            <dbReference type="Rhea" id="RHEA-COMP:9707"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78536"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.1" evidence="15"/>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C3L5"/>
    <dbReference type="OrthoDB" id="337870at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004831">
        <property type="term" value="F:tyrosine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006437">
        <property type="term" value="P:tyrosyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00805">
        <property type="entry name" value="TyrRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.240.10:FF:000001">
        <property type="entry name" value="Tyrosine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.290.10:FF:000017">
        <property type="entry name" value="Tyrosine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000107">
        <property type="entry name" value="Tyrosine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="entry name" value="RNA-binding S4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.240.10">
        <property type="entry name" value="Tyrosyl-Transfer RNA Synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02006">
        <property type="entry name" value="Tyr_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002305">
        <property type="entry name" value="aa-tRNA-synth_Ic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054608">
        <property type="entry name" value="SYY-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002307">
        <property type="entry name" value="Tyr-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024088">
        <property type="entry name" value="Tyr-tRNA-ligase_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024107">
        <property type="entry name" value="Tyr-tRNA-ligase_bac_1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00234">
        <property type="entry name" value="tyrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11766:SF0">
        <property type="entry name" value="TYROSINE--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11766">
        <property type="entry name" value="TYROSYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22421">
        <property type="entry name" value="SYY_C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00579">
        <property type="entry name" value="tRNA-synt_1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01040">
        <property type="entry name" value="TRNASYNTHTYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55174">
        <property type="entry name" value="Alpha-L RNA-binding motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 15" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 15 17" id="KW-0436">Ligase</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 15" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 14" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Tyrosine--tRNA ligase SYY-like C-terminal" evidence="16">
        <location>
            <begin position="404"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU361234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF22421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ8037565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="471" mass="52820" checksum="3DF9F944AC996AB3" modified="2023-09-13" version="1">MAASLNKLSNRLYRPFLQHLERRLLCCATSSTNSSGSNILSLEERGLFHDVIPAKSSLFLSKHLNSAPQCIYCGFDPTADSLHIGNLLTLLTLIRCQRAGHQVIALIGGATALIGDPSGKTTERPALTLPEVESNVKRLEDSLHRVFTNHERYFWKKQKELPRYRILNNYDWYKDVNLVSFLSSAGRHFRMHKLLSRESVQARLSSPEGMSFQEFSYQIFQAYDFYHLHQKYHCLLQIGGSDQLGNLSTGHDFVKRVTGKDVYAITIPLITNSQGEKLGKTAGNAVWISADRTSPFELYQTFVRLQDADVEKYLKAFTFLSLPEIDAVLKNHKANPKKRSAQKLIAEQVVLLVHGENGLDQALRLTEALYNSGPSTLEALSPMELDSLFEGALTHEALLEPGTTVLDLVEMVQCIPSGQNAEKLISEGGLYVNQVRVANPQAVLKKGEHILKNGITLIRVGKKKYFIVKWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C4B8</accession>
    <name>A0A9Q1C4B8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_18591</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C4B8"/>
    <dbReference type="OrthoDB" id="10029846at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8037701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="113" mass="12908" checksum="F9FF36ACC9D5ED73" modified="2023-09-13" version="1">MRPTDPVDLAFDIDKRHTPTGFLWADIIVGENRRIMFATDEQLNLLGKAKVWYVDATFNHHSPNFSPSMHSSRRVKVASATAVRHGVRQEEEGLQKDTKKSCKAFYQTNGFSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C4D7</accession>
    <name>A0A9Q1C4D7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SCAN box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_18887</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C4D7"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="CDD" id="cd22249">
        <property type="entry name" value="UDM1_RNF168_RNF169-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF13">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCAN box" evidence="2">
        <location>
            <begin position="260"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="354"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="622"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="354"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="369"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="396"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="622"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="645"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="665"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="690"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="713"/>
            <end position="725"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8037940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="747" mass="84732" checksum="A69532EFC6E27C9F" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C7F2</accession>
    <name>A0A9Q1C7F2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_16881</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C7F2"/>
    <dbReference type="OrthoDB" id="8060926at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46704:SF9">
        <property type="entry name" value="BHLH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46704">
        <property type="entry name" value="CXC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8039231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="84" mass="9425" checksum="5F60C534257265C2" modified="2023-09-13" version="1">MDEATHVKAQQIRWANDVFKRRTVMRLGEFHTTMAYLACTGSQFGEAGLRDVLTEADVVAQSSIYGVLNSHHYNRSASVRAHKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C9L1</accession>
    <name>A0A9Q1C9L1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_12017</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C9L1"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8041252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="56" mass="6465" checksum="21F67BA39FDBCC3E" modified="2023-09-13" version="1">MCSTRLKPLPRYCSYYHYLPVAPPREAQQQKLKAAYKFSGKRFGFVIGIGLLGNWN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C9M0</accession>
    <name>A0A9Q1C9M0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ferric-chelate reductase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_12667</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C9M0"/>
    <dbReference type="OrthoDB" id="2419613at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900449">
        <property type="term" value="P:regulation of glutamate receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099072">
        <property type="term" value="P:regulation of postsynaptic membrane neurotransmitter receptor levels"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005018">
        <property type="entry name" value="DOMON_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042789">
        <property type="entry name" value="FRRS1L"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46902">
        <property type="entry name" value="DOMON DOMAIN-CONTAINING PROTEIN FRRS1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46902:SF1">
        <property type="entry name" value="DOMON DOMAIN-CONTAINING PROTEIN FRRS1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03351">
        <property type="entry name" value="DOMON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50836">
        <property type="entry name" value="DOMON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040387995" evidence="2">
        <location>
            <begin position="23"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="207"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="DOMON" evidence="3">
        <location>
            <begin position="52"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8041763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="226" mass="25031" checksum="B49A016C47EA632D" modified="2023-09-13" version="1">MGYIFASVVITAYLVCLPPVHCMITKDTGVCGSTKSCYSIPDGCNPDVAGSCRAFSSWSLTADNTSLLINLWSNTTTYIALGFSKSGEMKDSDVYACTFDMEVLRSRNEEDENDNFPQELIGVTNPSVMFDQGIISCSFLRVLENDTYDDTFFDLTCPNKYTPILAWGGAVRDSRIFEHTDRGHGHEEISFDERCSSGNGLHPSTKLHGPVIFFALLSSFLVIFFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CAE1</accession>
    <name>A0A9Q1CAE1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_11764</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CAE1"/>
    <dbReference type="OrthoDB" id="2687452at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000072">
        <property type="entry name" value="zinc finger protein 143 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000534">
        <property type="entry name" value="zinc finger protein 674"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022755">
        <property type="entry name" value="Znf_C2H2_jaz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24393:SF34">
        <property type="entry name" value="PR_SET DOMAIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24393">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12171">
        <property type="entry name" value="zf-C2H2_jaz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="236"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="584"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="651"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="677"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="705"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="733"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="762"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="792"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="820"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="357"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="451"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="502"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="363"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="454"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="502"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="521"/>
            <end position="544"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8041061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1016" mass="115397" checksum="38BAAE2DE1A0265A" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CAV9</accession>
    <name>A0A9Q1CAV9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_12894</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CAV9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8041951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="51" mass="5482" checksum="E59376D1A2165D42" modified="2023-09-13" version="1">MADVEQHKPAGKPSRLKAGAGKRQSSADPKNKSRLAALAPGENLKEKQVRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CB82</accession>
    <name>A0A9Q1CB82_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Low-density lipoprotein receptor-related protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_13056</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8042077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CB82"/>
    <dbReference type="OrthoDB" id="19606at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042333">
        <property type="entry name" value="LRAD2/Mig-13-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24652:SF69">
        <property type="entry name" value="CUB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24652">
        <property type="entry name" value="LOW-DENSITY LIPOPROTEIN RECEPTOR CLASS A DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="221"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="5">
        <location>
            <begin position="44"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="343"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="351"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="383"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="172"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8042077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="464" mass="52422" checksum="4E538C77D9ED8D1A" modified="2023-09-13" version="1">MKTKATLHQPTSLSQWRRYWGLFLVVSSFFVGSVTATVYMNKHCHGSITSRGDIIKSQDTLYYEPNSKCELILMAGGNSKILLQILDINIVPSGSNCHASYLEVFPVAGSSKNLFLCNRSTVPKLITSKGSYLRLRLVNQGDRVGRGFYGVFATTYPSGKPLSCRYPEYHQCSNGYCISHDLTCDTVNHCGDNSDEDPSLCKEEVYRWMELSKGPISVVEIVFTLIGLGIVITVVVVVVSNCCIDFQRRRRQTYDMSDSVLGETFLRKDSVSYHPAGSRVPIQITRSASIDYSARTATQEDLQYLQKLSESDDVFLDSDLESNSSEARKKISVRREQINQEIEDKVRDINRNNVKQTETDSPSSQKQKGKLPRRVSFDEQVPPNLNNLQEKSESVASKQKTKKKLQRSVSFDDQVSANLNYTEEREESLALLQQPSIQQERRRKPGVIMEHDEGAFAEVFETEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CBH5</accession>
    <name>A0A9Q1CBH5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_09435</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8042633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CBH5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8042633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="80" mass="9224" checksum="A31663FC0B668C15" modified="2023-09-13" version="1">MPTSLEKQCTVDYCPVLPKNGNGPTVYSQSFDQRITFPVKTQRRRRSFIIERESIQVGQTVFCGESEKLMAYQRHRPSLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CBT4</accession>
    <name>A0A9Q1CBT4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Mitochondrial ribosome-associated GTPase 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_12764</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CBT4"/>
    <dbReference type="OrthoDB" id="347018at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01898">
        <property type="entry name" value="Obg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.210.12:FF:000001">
        <property type="entry name" value="GTPase Obg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.210.12">
        <property type="entry name" value="GTP1/OBG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01454">
        <property type="entry name" value="GTPase_Obg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014100">
        <property type="entry name" value="GTP-bd_Obg/CgtA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006169">
        <property type="entry name" value="GTP1_OBG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036726">
        <property type="entry name" value="GTP1_OBG_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045086">
        <property type="entry name" value="OBG_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02729">
        <property type="entry name" value="Obg_CgtA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008956">
        <property type="entry name" value="PRK12299.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11702">
        <property type="entry name" value="DEVELOPMENTALLY REGULATED GTP-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11702:SF31">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOME-ASSOCIATED GTPASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01018">
        <property type="entry name" value="GTP1_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002401">
        <property type="entry name" value="GTP_bd_Obg/CgtA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82051">
        <property type="entry name" value="Obg GTP-binding protein N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51883">
        <property type="entry name" value="OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Obg" evidence="7">
        <location>
            <begin position="36"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="OBG-type G" evidence="6">
        <location>
            <begin position="191"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="358"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8041843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="382" mass="41838" checksum="2240B0AD81D73433" modified="2023-09-13" version="1">MQKFLQRTTRVCKLCQCGMATKRKALGKPDKSEKKMNFVDWKRVEFFGGKGGDGCVSMRREAFAAFGGPDGGDGGTGGDIILEAATNVKSLEHVSHIYKGMNGEGGRGQNCSGKSGKHTIIQVPLGTILKENDKMLCDLEKSGDQVVVVHGGRGGRGNKSFVTSQMQAPLVATRGQQGQHMKLSLELRTIAHAGLVGFPNAGKSSLLRVLSRARPKVAAYPFTTLNPHVGIIEYSDLEQVAVADIPGLIPGAHLNKGLGHSFLRHIERCLCLIYVIDLSLKDPWQQLTHLQKELELYQPGLSERPHAIIGNKIDLPESQQNLPGLQERVALPVIALSAKCNTNIGALKKHIRELYDSDMKDKRRQDDGENVKQKKTNRDRVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CD14</accession>
    <name>A0A9Q1CD14_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_10382</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CD14"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8043338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="99" mass="11812" checksum="C8C6FA1E13C522A2" modified="2023-09-13" version="1">MDAINQKLRRLNMSDEKRTEYNKKAAIRMANMRRRKAEAKATEAAKGHPVKIKTMTRAESIKETEKRRKAAAYKREQRAKLTTRQKEEINRKRREKARK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CEJ7</accession>
    <name>A0A9Q1CEJ7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF7869 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_11185</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CEJ7"/>
    <dbReference type="OrthoDB" id="410478at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057191">
        <property type="entry name" value="DUF7869"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33153">
        <property type="entry name" value="MYND-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33153:SF3">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 11 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25273">
        <property type="entry name" value="DUF7869"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7869" evidence="1">
        <location>
            <begin position="2"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF25273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8043886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="186" mass="22277" checksum="91B6AFFD362AEA83" modified="2023-09-13" version="1">MYVLFLPVGHIHEDVDQMFSCVEQHLRHTNVCTLEDLAKEAAKSFTPNTDVRPVITVQDVKALFSQHLYSQFANHSRPLWFRFRKVDKRCYMHFKKWECEMWEPESCEEYPHGYLVLKDVPELHQRVEWVMSNLERLDVEKLTKELPEAYRHRLPSAAQHWWAKFISNLSSTYEVLSSFWCSSHVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CER6</accession>
    <name>A0A9Q1CER6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Carbohydrate sulfotransferase</fullName>
            <ecNumber evidence="9">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_10447</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Golgi apparatus membrane</location>
            <topology evidence="1 9">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the sulfotransferase 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="9"/>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CER6"/>
    <dbReference type="OrthoDB" id="2019940at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016051">
        <property type="term" value="P:carbohydrate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018011">
        <property type="entry name" value="Carb_sulfotrans_8-10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005331">
        <property type="entry name" value="Sulfotransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12137">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12137:SF54">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03567">
        <property type="entry name" value="Sulfotransfer_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="8 9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8043388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="347" mass="40252" checksum="6BFD05BD277F27AD" modified="2023-09-13" version="1">MGGKKLHFLVFTCTTITMMLLITSYGIIPSSDLLKSQVLRGDNETMNVLRPKTAKQVANITEIWKQQKVRLIRLKTVCENLNRTSSFTPPEDYTARLLVNDHDGVLFGYIPKVGCKTWKGIFSESNKAYGQKPFLTLSEYNVAERTKRLQRYKKVLFVRDPLIRFLSAYLSKFRNFKNLQKTWEKMFGFKILESYRPGSEELLKKTRALPIDERPFLNVTLTEFIRFITDLGDTYVLHAISDHWLPQHIVSHVCEIPYDFIGKYENLASEAPFVLNWVGMASVVQFPEIHLSKAATNLVYEYSNISLDVIEDLRKYYSLDFELFGYDSTETIAKVTRDIFNMKILDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CHF3</accession>
    <name>A0A9Q1CHF3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">WD repeat-containing protein on Y chromosome</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_08383</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CHF3"/>
    <dbReference type="OrthoDB" id="5980302at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051242">
        <property type="entry name" value="WD-EF-hand_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44324:SF6">
        <property type="entry name" value="EF-HAND CALCIUM BINDING DOMAIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44324">
        <property type="entry name" value="WD40 REPEAT DOMAIN 95"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101908">
        <property type="entry name" value="Putative isomerase YbhE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="7">
        <location>
            <begin position="60"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="7">
        <location>
            <begin position="98"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="389"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="425"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="514"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="573"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="651"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="1023"/>
            <end position="1056"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="730"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1127"/>
            <end position="1161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="32"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="731"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="777"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="811"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="827"/>
            <end position="843"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00014901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8045382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1266" mass="142726" checksum="6A395FFE231635B9" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-06-18" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CJQ6</accession>
    <name>A0A9Q1CJQ6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Polycystic kidney disease 1-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_08932</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10068766at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.220.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057244">
        <property type="entry name" value="GAIN_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046338">
        <property type="entry name" value="GAIN_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000203">
        <property type="entry name" value="GPS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002859">
        <property type="entry name" value="PKD/REJ-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02010">
        <property type="entry name" value="REJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00303">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50221">
        <property type="entry name" value="GAIN_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040376000" evidence="8">
        <location>
            <begin position="23"/>
            <end position="2836"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="2648"/>
            <end position="2678"/>
        </location>
    </feature>
    <feature type="domain" description="GAIN-B" evidence="9">
        <location>
            <begin position="2464"/>
            <end position="2627"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1730"/>
            <end position="1750"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="2813"/>
            <end position="2836"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1735"/>
            <end position="1745"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="2814"/>
            <end position="2836"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8045834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="2836" mass="316266" checksum="ED08C6FCBF2CA1A7" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CL75</accession>
    <name>A0A9Q1CL75_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pre-mRNA-splicing factor 18</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">PRP18 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_05503</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus speckle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PRP18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CL75"/>
    <dbReference type="OrthoDB" id="10261918at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071021">
        <property type="term" value="C:U2-type post-spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000350">
        <property type="term" value="P:generation of catalytic spliceosome for second transesterification step"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.940.10:FF:000002">
        <property type="entry name" value="Pre-mRNA processing factor 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.110:FF:000001">
        <property type="entry name" value="pre-mRNA-splicing factor 18 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.940.10">
        <property type="entry name" value="Functional domain of the splicing factor Prp18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.110">
        <property type="entry name" value="Pre-mRNA processing factor 4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004098">
        <property type="entry name" value="Prp18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014906">
        <property type="entry name" value="PRP4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036285">
        <property type="entry name" value="PRP4-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039979">
        <property type="entry name" value="PRPF18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13007">
        <property type="entry name" value="PRE-MRNA SPLICING FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13007:SF19">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02840">
        <property type="entry name" value="Prp18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08799">
        <property type="entry name" value="PRP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00500">
        <property type="entry name" value="SFM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47938">
        <property type="entry name" value="Functional domain of the splicing factor Prp18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158230">
        <property type="entry name" value="PRP4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Pre-mRNA processing factor 4 (PRP4)-like" evidence="10">
        <location>
            <begin position="77"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="40"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8046730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="338" mass="39441" checksum="2605DB743319CE21" modified="2023-09-13" version="1">MDFLKAEIARKKRQLETDVSSSASKKNFFKRGDLIAKQKEDYFKRQKRDDSKDQTDEGGNSRKRIDSTDQDNKEVTYSRQEVIRRLRERGEPIRLFGETNSEAFYRLRRIEMLAPEINKGLRNDFKDAMDKVDQQYLEEMVKGHGEDEHTKAYDIKLHEEGGTTLEELVELSRQVGTEETDNELIIKFIRFVQRKWAEDLNSRETENKRNMKGKLQTATYSQTSSYLEPLVRKLKKKNLSPDLLDSLVQIVKFMLEREYVKANDAYLQMAIGNAPWPIGVTMVGIHARTGREKIFSKNVAHVLNDETQRKYIQGLKRLMTICQRLFPTDPSKSVEYGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CM18</accession>
    <name>A0A9Q1CM18_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Glutamine--tRNA ligase</fullName>
            <ecNumber evidence="3">6.1.1.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Glutaminyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">HOLleu_06781</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="24">Nanhai2018</strain>
            <tissue evidence="24">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Glutamine--tRNA ligase. Plays a critical role in brain development.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>tRNA(Gln) + L-glutamine + ATP = L-glutaminyl-tRNA(Gln) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:20121"/>
            <dbReference type="Rhea" id="RHEA-COMP:9662"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.18"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="15">Monomer. Part of a multisubunit complex that groups tRNA ligases for Arg (RARS1), Asp (DARS1), Gln (QARS1), Ile (IARS1), Leu (LARS1), Lys (KARS1), Met (MARS1) the bifunctional ligase for Glu and Pro (EPRS1) and the auxiliary subunits AIMP1/p43, AIMP2/p38 and EEF1E1/p18. Interacts with RARS1. Part of a complex composed of RARS1, QARS1 and AIMP1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.18" evidence="3"/>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CM18"/>
    <dbReference type="OrthoDB" id="10250478at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017101">
        <property type="term" value="C:aminoacyl-tRNA synthetase multienzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004819">
        <property type="term" value="F:glutamine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006425">
        <property type="term" value="P:glutaminyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00807">
        <property type="entry name" value="GlnRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.1290:FF:000001">
        <property type="entry name" value="Glutamine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2420:FF:000001">
        <property type="entry name" value="Glutamine--tRNA ligase cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.240.10:FF:000008">
        <property type="entry name" value="probable glutamine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000049">
        <property type="entry name" value="Probable glutamine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.240.10:FF:000006">
        <property type="entry name" value="Putative glutamine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.1290">
        <property type="entry name" value="Glutaminyl-tRNA synthetase, non-specific RNA binding region part 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.240.10">
        <property type="entry name" value="Ribosomal Protein L25, Chain P"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004514">
        <property type="entry name" value="Gln-tRNA-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007638">
        <property type="entry name" value="Gln-tRNA-synth_Ib_RNA-bd_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007639">
        <property type="entry name" value="Gln-tRNA-synth_Ib_RNA-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042558">
        <property type="entry name" value="Gln-tRNA-synth_Ib_RNA-bd_N_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042559">
        <property type="entry name" value="Gln-tRNA-synth_Ib_RNA-bd_N_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050132">
        <property type="entry name" value="Gln/Glu-tRNA_Ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000924">
        <property type="entry name" value="Glu/Gln-tRNA-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020058">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020059">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_codon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020056">
        <property type="entry name" value="Rbsml_bL25/Gln-tRNA_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011035">
        <property type="entry name" value="Ribosomal_bL25/Gln-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049437">
        <property type="entry name" value="tRNA-synt_1c_C2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00440">
        <property type="entry name" value="glnS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43097:SF4">
        <property type="entry name" value="GLUTAMINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43097">
        <property type="entry name" value="GLUTAMINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00749">
        <property type="entry name" value="tRNA-synt_1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03950">
        <property type="entry name" value="tRNA-synt_1c_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20974">
        <property type="entry name" value="tRNA-synt_1c_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04558">
        <property type="entry name" value="tRNA_synt_1c_R1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04557">
        <property type="entry name" value="tRNA_synt_1c_R2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00987">
        <property type="entry name" value="TRNASYNTHGLU"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50715">
        <property type="entry name" value="Ribosomal protein L25-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="11 17" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="8 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 17 24" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9 17" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutaminyl-tRNA synthetase class Ib non-specific RNA-binding" evidence="22">
        <location>
            <begin position="8"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="Glutaminyl-tRNA synthetase class Ib non-specific RNA-binding" evidence="21">
        <location>
            <begin position="167"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamyl/glutaminyl-tRNA synthetase class Ib catalytic" evidence="19">
        <location>
            <begin position="270"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamyl/glutaminyl-tRNA synthetase class Ib anti-codon binding" evidence="20">
        <location>
            <begin position="572"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA synthetases class I (E and Q) anti-codon binding" evidence="23">
        <location>
            <begin position="684"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="182"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="185"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00058106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00062609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00073804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU363037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF03950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF04557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF04558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF20974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="KAJ8047718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="782" mass="88867" checksum="3DDC3AB68E202CCB" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CNI3</accession>
    <name>A0A9Q1CNI3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Helitron helicase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_00830</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CNI3"/>
    <dbReference type="OrthoDB" id="10064798at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025476">
        <property type="entry name" value="Helitron_helicase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14214">
        <property type="entry name" value="Helitron_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helitron helicase-like" evidence="1">
        <location>
            <begin position="2"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8048491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="94" mass="11028" checksum="761BB2AFFD98287D" modified="2023-09-13" version="1">MFEHRVQAFMKDVILSPAQPIGHVIDYFYRAEFQQRGWPHIHCLFWVKDAPLYGNSNTDEIVAFIDKYVSCKMPSEATEPKLHEKVLHVQMHNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CQ26</accession>
    <name>A0A9Q1CQ26_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_01999</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CQ26"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
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    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="24"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8049308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="66" mass="7326" checksum="4E4D2541224BB240" modified="2023-09-13" version="1">MFSLTAIFINVLIASVPIPQNYQTPLSTLLILMNMIIVLVVAGKNIPLFSSKALSFINWLTNTYVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CR10</accession>
    <name>A0A9Q1CR10_HOLLE</name>
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        <submittedName>
            <fullName evidence="6">Sodium-dependent glucose transporter 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_01576</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
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        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome 1"/>
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        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
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        <property type="entry name" value="SODIUM-DEPENDENT GLUCOSE TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23121:SF9">
        <property type="entry name" value="SODIUM-DEPENDENT GLUCOSE TRANSPORTER 1"/>
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    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter"/>
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    </dbReference>
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    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="1 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
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            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
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    </feature>
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    </feature>
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            <begin position="827"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="856"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="881"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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            <end position="939"/>
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    </feature>
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            <end position="745"/>
        </location>
    </feature>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8049029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1026" mass="113262" checksum="AD479BA8D4025F69" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CRG8</accession>
    <name>A0A9Q1CRG8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_03052</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CRG8"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040356709" description="Secreted protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8050028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="168" mass="19100" checksum="124F04AA7155528E" modified="2023-09-13" version="1">MCVCVCVCTWVCVCVTLASVHCNSTRASWTKLKLGIWICHSEKVCPIVFGGGQRSCGVTRGQRPKTLFARYLKVGSLDKFHTWYVDALWREDECRSFWWRSKVIWGHQQSNTENLLLKQYLKIQHSEKLCHTVLGGGQSSSGVTKGQKLITLLLAQYLKVGSFDKFHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CS00</accession>
    <name>A0A9Q1CS00_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">F-box only protein 30</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_02695</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Echinodermata</taxon>
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            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="OrthoDB" id="5918172at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22101">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.150">
        <property type="entry name" value="TRAF-like zinc-finger, N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031890">
        <property type="entry name" value="Fbxo30/Fbxo40"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001293">
        <property type="entry name" value="Znf_TRAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043013">
        <property type="entry name" value="Znf_TRAF_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15933:SF20">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15933">
        <property type="entry name" value="PROTEIN CBG16327"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
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    </dbReference>
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    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="4 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 5" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="55"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="F-box" evidence="9">
        <location>
            <begin position="545"/>
            <end position="599"/>
        </location>
    </feature>
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        <location>
            <begin position="55"/>
            <end position="97"/>
        </location>
    </feature>
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            <begin position="268"/>
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        </location>
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            <begin position="482"/>
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        </location>
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        <location>
            <begin position="172"/>
            <end position="199"/>
        </location>
    </feature>
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        <location>
            <begin position="274"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="287"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="318"/>
            <end position="333"/>
        </location>
    </feature>
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        <location>
            <begin position="334"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="484"/>
            <end position="500"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8049793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="673" mass="76700" checksum="542EA3132052DE81" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CS60</accession>
    <name>A0A9Q1CS60_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">THAP-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_03116</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CS60"/>
    <dbReference type="OrthoDB" id="9903208at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006612">
        <property type="entry name" value="THAP_Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31751:SF42">
        <property type="entry name" value="PROTEIN CBG10204"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31751">
        <property type="entry name" value="SI:CH211-108C17.2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05485">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00692">
        <property type="entry name" value="DM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00980">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50950">
        <property type="entry name" value="ZF_THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="THAP-type" evidence="7">
        <location>
            <begin position="70"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8050065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="798" mass="90994" checksum="F329958F0776BB82" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CTM0</accession>
    <name>A0A9Q1CTM0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_03424</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CTM0"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8050284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="277" mass="31741" checksum="87CDDF28AC6B11DC" modified="2023-09-13" version="1">MLRTLCKGDAVKACIDGVEETVDVVEVDRFERTVVVEQHEQGQRLTKVKACLVSELEDETISEVTNSETSNAVATEWNSECNERMTFFKCPQTEVEVGEVVEATPLGIKMAVKEIYYDDEVFRCGGNVVNDGSLTDFALSGPVEKVKKCDNYKVQRQDRLLLNTIKHETFQECLLRSRKNFEYRKLIRLHQLRQLGRHEVRRALTGKARVRSFSFGLVSLDTDFELFEMDVLRKKKVFTPWNGCMHVLDAVLGEGWDLLKEANCFYFVTQFVISCTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CTY9</accession>
    <name>A0A9Q1CTY9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_04545</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8051100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CTY9"/>
    <dbReference type="OrthoDB" id="416454at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8051100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="55" mass="6518" checksum="B3939B093727064B" modified="2023-09-13" version="1">MLTAPRIRTKIGKKSFSWAAPYLWNNLPTLIRNITSLEVFKRTIKTHLFLHYLCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H0V0</accession>
    <name>A0A9Q1H0V0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Sorbitol dehydrogenase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Polyol dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_28852</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H0V0"/>
    <dbReference type="OrthoDB" id="1879366at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003939">
        <property type="term" value="F:L-iditol 2-dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006062">
        <property type="term" value="P:sorbitol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05285">
        <property type="entry name" value="sorbitol_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000068">
        <property type="entry name" value="Sorbitol dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045306">
        <property type="entry name" value="SDH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43161">
        <property type="entry name" value="SORBITOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43161:SF9">
        <property type="entry name" value="SORBITOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="10">
        <location>
            <begin position="15"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00026132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8029459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="352" mass="37112" checksum="4D79DD3F1C5AB4C0" modified="2023-09-13" version="1">MAPGDGNLSAVLKKKDELVLEDTPVPQPGKNEVQVQMHAVGICGSDVHYWTHGAIGDFIVRKPMILGHESSGIVSAVGEGVTHLKVGDRIAIEPGIPCRTCGFCKEGRYNLCPEMSFCATPPIDGSLRRYYCHAADFCYKLPDHVSLEEGALLEPLSVGVHACRRSGVTLGSKVLVCGAGPIGLVCLLTAKAMGAATVGITDIAENRLEMAKKLGANFTFKVDTKDGKEIARRIHSTMGCAADVTIECSGAPSSVQTGIYSTKSGGVLVLVGMGPAEITIPIIDASVREVDIRGIFRYANCYPTALAMIASGKVDAKPLITHHFPLAKTLEAFEVAKTGAGGAVKVIIHCNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H1B5</accession>
    <name>A0A9Q1H1B5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_28230</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8028965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H1B5"/>
    <dbReference type="OrthoDB" id="416454at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8028965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="215" mass="23565" checksum="7C20D7FDB47F269A" modified="2023-09-13" version="1">MAYLLTEAGGVVLTPMDVELGGKTSLCGILLNRIFGPTHVTGRAVPYTNCNYRSSLNVTTPTTLSVVAIYRPPYSAPHPVNANIFLTEFAGFINTILVDYKNVLTGGDFNIHVDVENDTDKSFLVIFFISKIQRLRDSMDGEELYIPPVHAGSLFTDSESCLGKSVYKIVSNLKSTTCVSDPLPTKYVKSNKDILLPLMTKIVDSSLRMGEFSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H1C7</accession>
    <name>A0A9Q1H1C7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Arylacetamide deacetylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_28993</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDXG' lipolytic enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H1C7"/>
    <dbReference type="OrthoDB" id="408631at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017157">
        <property type="entry name" value="Arylacetamide_deacetylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050300">
        <property type="entry name" value="GDXG_lipolytic_enzyme"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081">
        <property type="entry name" value="AB HYDROLASE SUPERFAMILY PROTEIN C4A8.06C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081:SF8">
        <property type="entry name" value="ALPHA_BETA HYDROLASE FOLD-3 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037251">
        <property type="entry name" value="Arylacetamide_deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="9"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="5">
        <location>
            <begin position="118"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="5">
        <location>
            <begin position="331"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="196"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="359"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR037251-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8029563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="419" mass="47824" checksum="9A0EA54FD6589A0D" modified="2023-09-13" version="1">MHNKSMRSYFFFVVLVTGAGISCWVNTPVPEGLAEPYKYRLLAAGSYLLGLQTSFLDVLWPDKGYDMIATRYIMNIGHREPPSIVPGSNFKARQTEFDGVTVRIYEPIRRTERLLPGFVFFHGGGYCLGNTKTYDGLTRQFADELDAVVVSVEYRLCPEYPFPSQFEDTFKVTRWFISHARDYGVDPTRIGIGGDSAGGHLAAVVTHHVHDDPSIPDPIVQLLVYPILQHVDLKTPSYQKYQHYFGNYGLFPIAKNARFQSYVIFGKSDPTFERYYQENNHTSIDFKMNSKVWDNLNHSVIPESLRDATFYSGPDEKNHGDKDTWERIKKLITDPRVNPFTRGDFRGLPPTLIQTCGFDSLRDDGIFYWTALKTAGVETKWINYESGVHGIFWLGGGMLEFDLGAKMRNDAINFMKSYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H1R4</accession>
    <name>A0A9Q1H1R4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">IgGFc-binding protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_28107</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8028861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H1R4"/>
    <dbReference type="OrthoDB" id="10005154at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035234">
        <property type="entry name" value="IgGFc-bd_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46534:SF1">
        <property type="entry name" value="IGGFC-BINDING PROTEIN N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46534">
        <property type="entry name" value="IGGFC_BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17517">
        <property type="entry name" value="IgGFc_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IgGFc-binding protein N-terminal" evidence="2">
        <location>
            <begin position="281"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="103"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8028861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="568" mass="62807" checksum="01B2AE9680E4F9ED" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H2F4</accession>
    <name>A0A9Q1H2F4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_26358</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H2F4"/>
    <dbReference type="OrthoDB" id="10035376at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8032727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="62" mass="6764" checksum="46B5D74F9C9CD51B" modified="2023-09-13" version="1">MGPSHGNDEMKLATTISVIVGTDMLCWLPIITLSVFSQSGIDIPTKVLNLGWLYLSFQSILP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H513</accession>
    <name>A0A9Q1H513_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_26363</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H513"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8032730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="97" mass="10513" checksum="36347D6499A2C355" modified="2023-09-13" version="1">MSKEISKFYTMLGKKRQSTKQRTGQLPSGRRLRDCPVPGCPAKGLKRVDGHLAAVHHLNKQSANYKLLLMQASSAADVPSTPAICIQANKSDPGPND</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H6E1</accession>
    <name>A0A9Q1H6E1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_18052</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H6E1"/>
    <dbReference type="OrthoDB" id="10042460at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="149"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="180"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="214"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="244"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="277"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="342"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="388"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="447"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8037272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="480" mass="56325" checksum="8B091971FF369DD0" modified="2023-09-13" version="1">MEMSLLGGDEEENRRSLFPWQQSIRLSDSKKSEWPHMIWFILKITGLFYHRNVVTKRWCISCEVDNYKSRLCTDQGGHKQTDLIMWDSIDLTTCYEHKRFDDELNLLSHQLEKYCKACETCWWDRYGKVSVYTEADIGAQAWNHRGSRLVSVVILLVVASLILFETIFTENKLFAKRELLLHFMSYTAFLCPLFVFPVMNICSKVRSALSGRVLGLWATSLNARYIVQRLQYLDLSKDGLPSKWFLVICLVWPICCSTYRAYITFALKGYLDIAHRLNCLTVGITEFMWGCFVYILFLMRLSFQIQLELVLDFIKKCEGDEDLCQRVVRRLTVDFRCFRDCVAVYMAMMIPLCVLGASTSITWQYLILAHIDKDCSSLWCVTERNINIMIWMDVLMFSSLGPLAIGGFNVDYLWENFQLHVKFMQNKIHQKFWNKIVRFLERTEDQVPYFSFTTILSVVSLYMAFNFDEKDIDITADIPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H8X4</accession>
    <name>A0A9Q1H8X4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_14408</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H8X4"/>
    <dbReference type="OrthoDB" id="6780773at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1820">
        <property type="entry name" value="L1 transposable element, RRM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35555">
        <property type="entry name" value="ENDONUCLEASE-REVERSE TRANSCRIPTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35555:SF3">
        <property type="entry name" value="ENDONUCLEASE-REVERSE TRANSCRIPTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="60"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="23"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="44"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8040182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="250" mass="28753" checksum="18C377A8982E5C86" modified="2023-09-13" version="1">MPQRGRPPGSLGKKKRDEIASQGTLRSLGSSLTTTLRNSREPTEEAYDDSEEEFTTENLATSLDNAMDEINKQLQKIRKDFSKAIADHDKVIEDLKTENLELKSKCGAHSELINKQERFSRRNNFRIVGLKTESDEDPIKMASEVISKIGIPNCKIERAHRDGRNVPGRDRHLLVKLSYYQDKVTIMKNARQSLSNESFYVIDDLTKMDLKEKRKWSQKVNQLFQQGTKLRFFGGCWRQANGKPYVFPTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HAQ6</accession>
    <name>A0A9Q1HAQ6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Serine/threonine-protein kinase PBL5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_16597</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HAQ6"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27001">
        <property type="entry name" value="OS01G0253100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27001:SF931">
        <property type="entry name" value="OS11G0664100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="197"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8039015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="384" mass="43815" checksum="934E43820B14A6A3" modified="2023-09-13" version="1">MDAATSSIELCKIIITCAKSLYKAGKNVETNFASQRGQLKTQIDDITMIVAQVKNTLEVQPTKYSDGLENVLQELKQSCLRVKAFFDDLDRYASYKGALFILKSVQHYFDIQNQFKDFNRVLPGQHKRLNNFILAGIYAKGGEKEEDINFERESKNEHTDLQSLISHVGENDTNVETAVRRKDALHRNSLSIRPDEISKEVALCKGSYGNYSTACLDKEKVMILRMWDDADDNTKNDFTREKRNLMFFSDGSIVRVIGYCDEPNVKFLVLEYMEQGKLSDVLHKSGKTLSLETKLKLAKTAAFALYRLHQKLVHTSLRTDMYLVDEHYTAKLSGLQYAKTFTSARRFVAENHPVVDVNRYIAPERFMPVKPTKAADIYGFGIVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HBU2</accession>
    <name>A0A9Q1HBU2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">HOLleu_14533</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="23">Nanhai2018</strain>
            <tissue evidence="23">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein-tyrosine phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="3"/>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HBU2"/>
    <dbReference type="OrthoDB" id="282898at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00047">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000038">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000102">
        <property type="entry name" value="Receptor-type tyrosine-protein phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001879">
        <property type="entry name" value="GPCR_2_extracellular_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024731">
        <property type="entry name" value="NELL2-like_EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050348">
        <property type="entry name" value="Protein-Tyr_Phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134:SF562">
        <property type="entry name" value="PROTEIN-TYROSINE-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134">
        <property type="entry name" value="RECEPTOR-TYPE TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12947">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50227">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 12" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="23" id="KW-0675">Receptor</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 16" id="KW-0732">Signal</keyword>
    <keyword evidence="13" id="KW-0768">Sushi</keyword>
    <keyword evidence="15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040459035" description="protein-tyrosine-phosphatase" evidence="16">
        <location>
            <begin position="30"/>
            <end position="1231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="466"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 1" evidence="20">
        <location>
            <begin position="133"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="22">
        <location>
            <begin position="148"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="208"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="21">
        <location>
            <begin position="253"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="21">
        <location>
            <begin position="359"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="18">
        <location>
            <begin position="682"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="19">
        <location>
            <begin position="855"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="18">
        <location>
            <begin position="964"/>
            <end position="1220"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="19">
        <location>
            <begin position="1143"/>
            <end position="1211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="570"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="604"/>
            <end position="615"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KAJ8040290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1231" mass="138481" checksum="875EBBD898491B96" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HC23</accession>
    <name>A0A9Q1HC23_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Pancreatic lipase-related protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_14884</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HC23"/>
    <dbReference type="OrthoDB" id="199913at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00707">
        <property type="entry name" value="Pancreat_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.60.20">
        <property type="entry name" value="PLAT/LH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013818">
        <property type="entry name" value="Lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016272">
        <property type="entry name" value="Lipase_LIPH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033906">
        <property type="entry name" value="Lipase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002331">
        <property type="entry name" value="Lipase_panc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001024">
        <property type="entry name" value="PLAT/LH2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036392">
        <property type="entry name" value="PLAT/LH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000734">
        <property type="entry name" value="TAG_lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610">
        <property type="entry name" value="LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610:SF173">
        <property type="entry name" value="LIPASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00151">
        <property type="entry name" value="Lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01477">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000865">
        <property type="entry name" value="Lipoprotein_lipase_LIPH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00823">
        <property type="entry name" value="PANCLIPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00821">
        <property type="entry name" value="TAGLIPASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49723">
        <property type="entry name" value="Lipase/lipooxygenase domain (PLAT/LH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040164753" evidence="8">
        <location>
            <begin position="17"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="domain" description="Lipase" evidence="9">
        <location>
            <begin position="18"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="PLAT" evidence="10">
        <location>
            <begin position="381"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="175"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="198"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="285"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000865-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000865-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8040559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="486" mass="54818" checksum="5250846205E18053" modified="2023-09-13" version="1">MKSLIFLSSIVLAVYANTVCYNELGCFSNEPPFRDMPDRPISYLPQSREDVGTTFLLNTPTNPAAYDYLSTYDLTSITNSNLNPFLHTKVIVHGYTENGYVDWMREMMEACLQQGNYNVIRVNWSKGAVGLYGVSTSNTRIVGAEISLLMDKIRVEVYPGTYVADPATWHIIGHSLGAHVSGYAGERQNGLGRITGMDPAGPYFEDTDLMVRLDPTDALFVDAIHTDTDPIYTIGLGIHMEVGHMDFYVNGGREQPGCDQGLIERIVFGDGGLYDAGVQFVACNHLRSYEYFTESILSSCPFGFRKATSLDYDNYFVPHSFYDENSGAKMGFYADQYKPPPGVTNVIYMGDTMDCAPYCAYQHRAKVRFDDPNLRTDDAVGDIYLRLKGSKGESYQMVGSGTYKPGTETYFPVDLTVDVGTVYEVDFYWEFDPEWYKPWEWSLFDNPDIYVSEIDLESLEFFQDYQFCGSSDKIKSNRDIRALTPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HCM5</accession>
    <name>A0A9Q1HCM5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Adhesion G-protein coupled receptor D1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_07213</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HCM5"/>
    <dbReference type="OrthoDB" id="10052455at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15040">
        <property type="entry name" value="7tmB2_Adhesion"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000058">
        <property type="entry name" value="Adhesion G protein-coupled receptor F5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.220.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057244">
        <property type="entry name" value="GAIN_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046338">
        <property type="entry name" value="GAIN_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12011">
        <property type="entry name" value="ADHESION G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12011:SF347">
        <property type="entry name" value="FI21270P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13385">
        <property type="entry name" value="Laminin_G_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00249">
        <property type="entry name" value="GPCRSECRETIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50221">
        <property type="entry name" value="GAIN_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040270276" evidence="10">
        <location>
            <begin position="21"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="554"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="588"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="617"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="656"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="693"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="740"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="766"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="domain" description="GAIN-B" evidence="11">
        <location>
            <begin position="400"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="12">
        <location>
            <begin position="552"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="815"/>
            <end position="843"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8044457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="843" mass="94239" checksum="41A9C993D714B1D8" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HD41</accession>
    <name>A0A9Q1HD41_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_15346</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HD41"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47018">
        <property type="entry name" value="CXC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47018:SF3">
        <property type="entry name" value="MYCBP-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="109"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8040903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="660" mass="73596" checksum="CA179B5809BFE952" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HD99</accession>
    <name>A0A9Q1HD99_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GMP reductase</fullName>
            <shortName evidence="6">GMPR</shortName>
            <ecNumber evidence="6">1.7.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Guanosine 5'-monophosphate oxidoreductase</fullName>
            <shortName evidence="6">Guanosine monophosphate reductase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_12713</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the irreversible NADPH-dependent deamination of GMP to IMP. It functions in the conversion of nucleobase, nucleoside and nucleotide derivatives of G to A nucleotides, and in maintaining the intracellular balance of A and G nucleotides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>IMP + NH4(+) + NADP(+) = GMP + NADPH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:17185"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.7.1.7"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the IMPDH/GMPR family. GuaC type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.7.1.7" evidence="6"/>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HD99"/>
    <dbReference type="OrthoDB" id="418595at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902560">
        <property type="term" value="C:GMP reductase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003920">
        <property type="term" value="F:GMP reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006144">
        <property type="term" value="P:purine nucleobase metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006163">
        <property type="term" value="P:purine nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00381">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00596">
        <property type="entry name" value="GMP_reduct_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050139">
        <property type="entry name" value="GMP_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005993">
        <property type="entry name" value="GMPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001093">
        <property type="entry name" value="IMP_DH_GMPRt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43170">
        <property type="entry name" value="GMP REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43170:SF5">
        <property type="entry name" value="GMP REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00478">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000235">
        <property type="entry name" value="GMP_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01240">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51412">
        <property type="entry name" value="Inosine monophosphate dehydrogenase (IMPDH)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 6" id="KW-0521">NADP</keyword>
    <keyword evidence="4 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3 6 7" id="KW-0630">Potassium</keyword>
    <keyword evidence="1 6" id="KW-0659">Purine metabolism</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IMP dehydrogenase/GMP reductase" evidence="8">
        <location>
            <begin position="10"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="IMP dehydrogenase/GMP reductase" evidence="8">
        <location>
            <begin position="212"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="26"/>
            <end position="27"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <note>ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="6">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <note>ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="6">
        <location>
            <begin position="135"/>
            <end position="137"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <note>ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="6">
        <location>
            <begin position="175"/>
            <end position="176"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <note>ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="239"/>
            <end position="241"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="262"/>
            <end position="263"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="288"/>
            <end position="290"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="6">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <note>ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="6">
        <location>
            <begin position="305"/>
            <end position="306"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <note>ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="306"/>
            <end position="310"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="334"/>
            <end position="337"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <note>ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000235-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8041805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="368" mass="39942" checksum="047F06A67464E527" modified="2023-09-13" version="1">MPRIDNDVKLDFKDVLLRPKRSTLKSRSEVEAIRRVDLKRSFTFRNSKQTYDGIPVVAANMDTVGTFQMAKALAKSGLFTCIHKHYSIEAWTEFANNNPGILEHVAVSSGIGENDFKKLCDIIEAVPGLRYICLDVANGYSELFVEFLKQAGNVVTGEMVEELILAGADIIKVGIGPGESWSPFTQVSVEENEAFEIKVIWNMIKYMAIKKAGVGYPQLSAVIECADAAHGLGGHIVSDGGCTCPGDVVKAFGAGADFVMLGGMFSGHDESDGEIIHRNGKKMKLFYGMSSATAMNKHSGGVAEYRASEGKTVEVPYKGEVIDTVLDILGGLRSACTYVGAGKLKELSRRTTFIRVTQQTNNIFGSTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HDH2</accession>
    <name>A0A9Q1HDH2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Testis-specific serine/threonine-protein kinase 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HOLleu_11933</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Nanhai2018</strain>
            <tissue evidence="18">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HDH2"/>
    <dbReference type="OrthoDB" id="504170at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050321">
        <property type="term" value="F:tau-protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14162">
        <property type="entry name" value="STKc_TSSK4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000658">
        <property type="entry name" value="Protein CBG12184"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047908">
        <property type="entry name" value="TSSK4_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF102">
        <property type="entry name" value="TESTIS-SPECIFIC SERINE_THREONINE-PROTEIN KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="9" id="KW-0221">Differentiation</keyword>
    <keyword evidence="8 18" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 14 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 15" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="13" id="KW-0744">Spermatogenesis</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="12" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="28"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="298"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="319"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ8041191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="357" mass="40389" checksum="0D7ADBE35C8445E6" modified="2023-09-13" version="1">MATEPKNSYNAKVNTKDKISNILETHGFIVSATIGHGSYATVKAAYSQSHKAKVAIKIVSKKKAPDDYISKFLPREIQVVKFLKHPNLVCFFQSIETTSRVYIAMELAENGDLLDFIKNNGSVAEAQTGVWFHQLVDGIEYCHQQGVVHRDLKCENLLLNKNNVLKITDFGFARNGMLSADANRPKMSETYCGSYAYAPPEILCGIPYNPMLSDIWSMGVVLFTMLFGRLPYDDTNHKILLHQVQRPPVFPSGKNVAEDAKDLLCRILSPAKRRIQMNEIREDHWFKRMTPKVLKKLSVQTKKDGSETVLQDMPNNETSKIKSEKQEKRESRTNKVVPESKAKKDPPDKKTEVTSVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HDI5</accession>
    <name>A0A9Q1HDI5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Adhesion G protein-coupled receptor L3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HOLleu_12113</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Nanhai2018</strain>
            <tissue evidence="15">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10034530at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000143">
        <property type="entry name" value="Protein crumbs 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.220.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057244">
        <property type="entry name" value="GAIN_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046338">
        <property type="entry name" value="GAIN_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000203">
        <property type="entry name" value="GPS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12011">
        <property type="entry name" value="ADHESION G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12011:SF471">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 2 PROFILE 2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01825">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13385">
        <property type="entry name" value="Laminin_G_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00249">
        <property type="entry name" value="GPCRSECRETIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00303">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50221">
        <property type="entry name" value="GAIN_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 10" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1777"/>
            <end position="1796"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1808"/>
            <end position="1827"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1839"/>
            <end position="1857"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1877"/>
            <end position="1898"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1918"/>
            <end position="1940"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1960"/>
            <end position="1983"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1989"/>
            <end position="2010"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="67"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="105"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="GAIN-B" evidence="13">
        <location>
            <begin position="1590"/>
            <end position="1762"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="14">
        <location>
            <begin position="1771"/>
            <end position="2012"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="71"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="93"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="131"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ8041323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="2076" mass="234178" checksum="5FCCB7B3066DF25A" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HEI7</accession>
    <name>A0A9Q1HEI7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_13230</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8042223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HEI7"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8042223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="67" mass="7854" checksum="DC525E8EDFB059A8" modified="2023-09-13" version="1">MPITSSLMQARKSAYSAYNAQMAEEQEKKAKEQQLKKRKGKETQDLQEAIKSLERKKQKLEDREKLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HEV6</accession>
    <name>A0A9Q1HEV6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_10439</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HEV6"/>
    <dbReference type="OrthoDB" id="10041847at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8043380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="198" mass="23404" checksum="D7BBD468F04DF1D7" modified="2023-09-13" version="1">MLVPQIHHWSVAYFPPKRVTFPSEDTFFYKKDINDNTEQVVYERDVTVSDFNGPAYLLREDKTPLSQNVQETVAISTTPAYERYFEGSSIEFYNDRRYEQEVRYLPGIPPRKSFSSALCYITANGRKYRWFQETVYLRFDLPPAKSFTTKVIIYPRRKVESHVSGVEHGDEQVIKYYRVAMDLQLRDYISLELVPLDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HF67</accession>
    <name>A0A9Q1HF67_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_06478</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HF67"/>
    <dbReference type="OrthoDB" id="6162977at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46601:SF1">
        <property type="entry name" value="ADF-H DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46601">
        <property type="entry name" value="ULP_PROTEASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="282"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8047472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="337" mass="37110" checksum="5C6C8A12797B9709" modified="2023-09-13" version="1">MIFISDDRVHDQHAVQHFITIATEHLAHQGIPMIRQVHFSDGAGSQYKNKVSFNDASFGMDDFGFPIEKHFFGSGHGKGPCDAEIGVVKKCASRAVLARQVIIANAHDLYKFGRDYLTLPKGTQEEHTHAKRKFFFVRDGEVKRGDERIARTEGLKPVPQTRAMHSFVGEKPNIVSARERSCFCEMCIAKDHASCANTNFLGTWKTFSLKGRGEVFNKPLEVDDSILPTLVNLMPVTNNCSYGISTCATSAEILTSSSDPISLVSESAGPCPDVTNSLQTEMENGNSGKVMTGSPSTQPQPGTLSSTGYLSTLQSSEKDVNRYLYFYNYHGVNILTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HFV0</accession>
    <name>A0A9Q1HFV0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">BRCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_00451</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HFV0"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16589">
        <property type="entry name" value="BRCT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRCT" evidence="1">
        <location>
            <begin position="176"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8048222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="294" mass="33039" checksum="0D76C147B0410E7B" modified="2023-09-13" version="1">MVDGKNPQNPPFVVHEEFLLYNVEYQLTGAVVMRPDHFFNVVKVGEQYALINGYPPMEDMHHYPKMFTSFSGAFRKDETDVSLTYTTDPANATGVHVLLYHCKEHNIEVSLMSVPNLFVDENNLGESSVDNQNCSKELLPEYGKSCSTKQMDSASQRNSPTAIPKIDGQSYSHVSLKALVESASGKIKDKLPSGNTSTKKYVVLVNPNATSKKNLSSEVRLALKAGYPVLDFSFVTNCLKEHQLLDMSKFMVDFSNHSQKFQSQPTLESKHFRRTPVMGFVYTLVLPTETFYEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HFV5</accession>
    <name>A0A9Q1HFV5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_00473</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
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        <property type="entry name" value="OUTER MEMBRANE PROTEIN PMPC-RELATED"/>
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            <dbReference type="EMBL" id="KAJ8048237.1"/>
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</entry>
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                <person name="Chen T."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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        <property type="entry name" value="INSULIN-LIKE GROWTH FACTOR-BINDING PROTEIN COMPLEX ACID LABILE SUBUNIT"/>
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    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="4"/>
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    </dbReference>
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        <property type="match status" value="16"/>
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    </feature>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HHT6</accession>
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            <taxon>Holothuria</taxon>
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            <authorList>
                <person name="Chen T."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
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    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
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        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
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        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
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        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23164">
        <property type="entry name" value="EARLY ENDOSOME ANTIGEN 1"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR23164:SF30">
        <property type="entry name" value="EARLY ENDOSOME ANTIGEN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
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    </dbReference>
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    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FYVE-type" evidence="7">
        <location>
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            <end position="1385"/>
        </location>
    </feature>
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        <source>
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        <source>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
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        </submittedName>
    </protein>
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        <name type="scientific">Holothuria leucospilota</name>
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            <taxon>Holothuriidae</taxon>
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        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
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    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="A0A9Q1HJ25"/>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="104"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8046723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="211" mass="24584" checksum="1D25D6AB1A512175" modified="2023-09-13" version="1">MCEQLGQDFYDQYVRIGILVEEEVLDISDDPNAIISEHIQYKTEVRFYHKLFCEWYAAHHLSDYASRDDVSIDPWEESKEAKRSYSLEESVNNETEEEPEYGNNIERQTDHYLKYLDPFDLQYVYRFSCGLNRVAADKIIGYLKTQTDAKKFAILCILEKSGRVKDVLKDVKELCSNQVDIDGGYSSLLQRSTIQLLEIALANKVSKRVQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HJK2</accession>
    <name>A0A9Q1HJK2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sulfotransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_06442</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HJK2"/>
    <dbReference type="OrthoDB" id="10010208at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053259">
        <property type="entry name" value="Golvesin-related_Golgi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005331">
        <property type="entry name" value="Sulfotransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32301">
        <property type="entry name" value="COUNTIN RECEPTOR CNR3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32301:SF6">
        <property type="entry name" value="GOLVESIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03567">
        <property type="entry name" value="Sulfotransfer_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8047446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="418" mass="47844" checksum="024E3B224E58ACB0" modified="2023-09-13" version="1">MKAHAIIDAKLSRKEQECLLNHSVDNVDQCFAEERGARANLGALGIIYSLPVPTKHKKTRQRVLSDPNKAIPKIKTKVLRIGSKLSGNNSWREALPWDIGPQRYLPNFYTYYAFLNESEKLLYRKGNIVYLHVPKSGGTTMAKCFKSMTKKLKRYSHFYGPIGENGINAVMEQLKTQNDTKLQIFSGEYAFYLCDLLGGKPCSYFLMLREPYDRLVSSYSFCKRHSDDGNNFYCSLNANNLTITEWALIQKSFFFRQLIMNEGMCQGHYDDEIDLNALMNIHKGVIKDKLPCWMKVQAILDSKLSKKEQEGLLKFIVDSIEQWFAGVGVLEKMPETLEALNIIYGLPVYPTCSKIHANKAPSKSKKEDEKVKERLMRELKSDPRVREALLPDLHLYKRVEQIVKSQSAARKHVLTGGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HKM8</accession>
    <name>A0A9Q1HKM8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Zinc finger protein 14</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HOLleu_02080</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Nanhai2018</strain>
            <tissue evidence="15">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HKM8"/>
    <dbReference type="OrthoDB" id="654211at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000744">
        <property type="entry name" value="zinc finger E-box-binding homeobox 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000624">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000585">
        <property type="entry name" value="zinc finger protein 784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24390:SF159">
        <property type="entry name" value="GROWTH FACTOR INDEPENDENT 1 TRANSCRIPTIONAL REPRESSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24390">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="125"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="153"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="181"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="209"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="237"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="265"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="293"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="321"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="53"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ8049365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="351" mass="40410" checksum="3EB5919810F490A8" modified="2023-09-13" version="1">MDKTEKEELPISSDELTCIKSEVEVESSDSAERYDVVIKVDLQNVVCVKEENETVEDKEDTLNETQKLSDETCRSELGTTNKTKGKSSKDSEDSCETTCNNHNNQTKLKSSVTKKTKDVNGTRSLNCRYCGKAFSRKNLLKTHEQKHSDKGPYKCMLCNTKIASKCNLKRHARTHSGERPFQCKQCDKTFAHSSNLVEHQLVHTNSRPFKCQYCGKAFKEQRKAAFHEKSHVGNKPFKCRICDKRYTLKQTLEGHERSHTGQKPYVCDYCDKSFAVQSKLARHKRSHTGEKPYQCQYCNKTFIRAYRVKEHEKLHASGRLLTCSVCKKTFQSDKVLRLHQKTHVQPERGKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HLE3</accession>
    <name>A0A9Q1HLE3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_02388</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HLE3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8049588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="176" mass="19923" checksum="D10E307B6EA3AE0E" modified="2023-09-13" version="1">MLRTGDGDRNVSSRLVTKNETYPFLTSRVTTTNAFAYSRVLTLLVCEAYCPLGLLEKNESLVLIQGSDETLPNILPTVVYAKQDTRVKLNCSEMTISYLVWQIKEPPGVIFKEVGHAVFVGDNLNHLYDDEYKLQNTSIVVDNVKVYHEGIYRCISGNGLQDDIIMYELIVFGKYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HM96</accession>
    <name>A0A9Q1HM96_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_04222</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HM96"/>
    <dbReference type="OrthoDB" id="8059659at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053134">
        <property type="entry name" value="RNA-dir_DNA_polymerase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24559:SF454">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24559">
        <property type="entry name" value="TRANSPOSON TY3-I GAG-POL POLYPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8050863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="218" mass="25001" checksum="DE22C3DB362D42F9" modified="2023-09-13" version="1">MPARCKKSRVVHVNLLKKRYSRGDEHRNAKFVFITGVVNEIGQAASEDYTEFSDNDVECNIVEANCIQIQTWQQAEITSDITSAQRCEVERVLNSYSEIFSDVPGRTVEHHIELVHREPVRQGAYRMPHAWRDPVKKELNQMLELGIIEPTTSTYASPIVVVPKPDGSVRVCTDYRRLNSKSKFDPYVMPRVDDILDKVGNAKFITTLDLTKGFYQIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YAD5</accession>
    <name>A0A9Q0YAD5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">BEN domain-containing protein 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_42621</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000101">
        <property type="protein sequence ID" value="KAJ8019043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YAD5"/>
    <dbReference type="OrthoDB" id="8858716at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045746">
        <property type="term" value="P:negative regulation of Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045666">
        <property type="term" value="P:positive regulation of neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2590">
        <property type="entry name" value="BEN domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018379">
        <property type="entry name" value="BEN_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037496">
        <property type="entry name" value="BEND6-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35346">
        <property type="entry name" value="BEN DOMAIN-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35346:SF1">
        <property type="entry name" value="BEN DOMAIN-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10523">
        <property type="entry name" value="BEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51457">
        <property type="entry name" value="BEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BEN" evidence="6">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8019043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="93" mass="10473" checksum="09CABABE62E61FB8" modified="2023-09-13" version="1">MVRKCNTHEPSGLVNDILTGLYTNEYLAAHSLTGTSAIDDSEFQLKERLDPTVVKEIVGTVMKHFPQKTPTDVKCFIRQKLNNASKAIKRKNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YEF5</accession>
    <name>A0A9Q0YEF5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_38359</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YEF5"/>
    <dbReference type="OrthoDB" id="5986443at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47018">
        <property type="entry name" value="CXC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47018:SF3">
        <property type="entry name" value="MYCBP-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="133"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="143"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8021223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="159" mass="18540" checksum="C562FAA12DC61814" modified="2023-09-13" version="1">MVQLLLHFIRATREGNWELHLSSTRSMIPWYFAYDRVNYARYLPAYWLEMCSLQKDHPAIYAEFRDGKFVAQRQRQHPFSQVACDQVIEQTVNRDSKTKGGLVGFSVNKGAVHRWILSQHERAAITKECLAMAGNEPSSGQKKHLDESRMKQDERDVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YF34</accession>
    <name>A0A9Q0YF34_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Splicing factor 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Mammalian branch point-binding protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Transcription factor ZFM1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Zinc finger gene in MEN1 locus</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Zinc finger protein 162</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="26" type="ORF">HOLleu_39887</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="26">Nanhai2018</strain>
            <tissue evidence="26">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Necessary for the ATP-dependent first step of spliceosome assembly. Binds to the intron branch point sequence (BPS) 5'-UACUAAC-3' of the pre-mRNA. May act as transcription repressor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the BBP/SF1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="26">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YF34"/>
    <dbReference type="OrthoDB" id="10021397at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048024">
        <property type="term" value="P:regulation of mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22382">
        <property type="entry name" value="KH-I_SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000016">
        <property type="entry name" value="Putative splicing factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045071">
        <property type="entry name" value="BBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055256">
        <property type="entry name" value="KH_1_KHDC4/BBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032570">
        <property type="entry name" value="SF1-HH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047086">
        <property type="entry name" value="SF1-HH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11208">
        <property type="entry name" value="RNA-BINDING PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11208:SF45">
        <property type="entry name" value="SPLICING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22675">
        <property type="entry name" value="KH-I_KHDC4-BBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16275">
        <property type="entry name" value="SF1-HH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="14" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="10 23" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="13" id="KW-0804">Transcription</keyword>
    <keyword evidence="12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 22" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="25">
        <location>
            <begin position="345"/>
            <end position="360"/>
        </location>
    </feature>
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            <begin position="1"/>
            <end position="32"/>
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    <feature type="region of interest" description="Disordered" evidence="24">
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            <begin position="883"/>
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        <location>
            <begin position="498"/>
            <end position="520"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="24">
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            <end position="550"/>
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    <feature type="compositionally biased region" description="Pro residues" evidence="24">
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        <location>
            <begin position="601"/>
            <end position="618"/>
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    <feature type="compositionally biased region" description="Gly residues" evidence="24">
        <location>
            <begin position="628"/>
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        <location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00055181"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00074240"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00075476"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00075641"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00082911"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00083224"/>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="EMBL" id="KAJ8020321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="942" mass="99250" checksum="31AEBCE57DA75EC6" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YGR8</accession>
    <name>A0A9Q0YGR8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phosphate transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_39713</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
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    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Sodium-phosphate symporter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome 22"/>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Phos_transporter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11101:SF80">
        <property type="entry name" value="PHOSPHATE TRANSPORTER"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF01384">
        <property type="entry name" value="PHO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
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            <end position="39"/>
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        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="503" mass="53647" checksum="0876167AA14CFA59" modified="2023-09-13" version="1">MIMAFDTSTSININLLIQSDVVWILIVGFLIAFVLSFAVGANDVANSFGTSVGANVLSLRQAFCLATIFETLGAVLLGSAVAKTISAEVVNLSLYNSTPEVLMVGQLAVLTACAVWLLIATALKLPVSTTHSIVGAMVGFQLVTVGTSGVQWRYLFYIAGSWIISPILAGGFAAFLNFILLYTIVKKEDPFHSGLKTLPAWYGVVILVNALSILLSAQNWFTTFEIPWYVIVAVSVGCAMITSFIVCIIVVPQLGNYDNEIYPLTNYEQLTPEEDRETDSHNPSDAINASESSNKSQETKGTNSSSTKQLDNGGLRSKQDAGQGPRTVREDPAVKKICGPLQILSAVFSAFAHGSNDVSNSVAPLIVLWNVYSYRAVPSELDISVWMFLYGGFGLSVGLWILGKRVIDTVGERITTLSSSRGFAIELGSAAVVLMASNIGIPISTTHSKIGAVAALGCFGNGEGVNLMLLCNIAVAWLVTLPFVAGLSAFITYIFQLSILPAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YH52</accession>
    <name>A0A9Q0YH52_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Angiopoietin-related protein 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_41970</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8020112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YH52"/>
    <dbReference type="OrthoDB" id="7250310at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00087">
        <property type="entry name" value="FReD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19941">
        <property type="entry name" value="TIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050373">
        <property type="entry name" value="Fibrinogen_C-term_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036084">
        <property type="entry name" value="Ser_inhib-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002919">
        <property type="entry name" value="TIL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025615">
        <property type="entry name" value="TILa_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040941">
        <property type="entry name" value="GGGWT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143:SF327">
        <property type="entry name" value="FI21813P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143">
        <property type="entry name" value="FIBRINOGEN/TENASCIN/ANGIOPOEITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01826">
        <property type="entry name" value="TIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12714">
        <property type="entry name" value="TILa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00186">
        <property type="entry name" value="FBG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57567">
        <property type="entry name" value="Serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040435716" evidence="1">
        <location>
            <begin position="29"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="2">
        <location>
            <begin position="48"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="2">
        <location>
            <begin position="313"/>
            <end position="559"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8020112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="560" mass="62548" checksum="983816BF8F8C50CB" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YK66</accession>
    <name>A0A9Q0YK66_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_37926</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YK66"/>
    <dbReference type="OrthoDB" id="6626162at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8022901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="70" mass="7679" checksum="C412CB8CDD1CC815" modified="2023-09-13" version="1">MLKISGNNEPILVADSYTLQDDSDGESEQDGVSSSSKSCKDCPLCCYKVLLKLNLLTDAYKSIELAYKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YLG8</accession>
    <name>A0A9Q0YLG8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_40785</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8021031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YLG8"/>
    <dbReference type="OrthoDB" id="5984753at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="2"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8021031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="135" mass="15600" checksum="AB208E5A885716DD" modified="2023-09-13" version="1">MFHQVLVPERDKHVHRFLWRSYDTKREPDVYVKEVVTFGDKPAPAMALTALKRTAEEGAKEYPEAARVLHENTFMDDICTSVHSREEAKKLIKDIDKVLQKGSFKVKEWVSNLNLGDGDTRPQQDNRLVFKSVSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YLY3</accession>
    <name>A0A9Q0YLY3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transmembrane protein 106 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_35021</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YLY3"/>
    <dbReference type="OrthoDB" id="508875at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009790">
        <property type="entry name" value="TMEM106"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048511">
        <property type="entry name" value="TMEM106_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28556:SF4">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 106A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28556">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 106B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21002">
        <property type="entry name" value="TMEM106_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="Transmembrane protein 106 N-terminal" evidence="3">
        <location>
            <begin position="43"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF21002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8024955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="268" mass="29388" checksum="30E8083A5226F040" modified="2023-09-13" version="1">MHTSENGSTKAGTLSDESEESCLHETRSNPPDYQSVTTGGTTLANGSSSSVSAYTYQELPGGVMCPTCNGTGRIPRGQEDDLVALIPYRDDRLKPRRTKLYVFLSILLCGLVAGIMCAFLLTKPVHMNIHNISTRSIQLQLNSSQQLHKLTLGLEITYNISNSNFPSIYLKNTTVIGMQSFNTQPVVSNTSAVDLRVSGRRHSQFAVNVTAVFSDDYVPQYCHSLQSKTITVEFSSTLQYQLMAQVSSISSSEYVRVLCAENEIPEIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YM01</accession>
    <name>A0A9Q0YM01_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Paraneoplastic antigen Ma-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_34967</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YM01"/>
    <dbReference type="OrthoDB" id="10065209at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026523">
        <property type="entry name" value="PNMA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048270">
        <property type="entry name" value="PNMA_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23095">
        <property type="entry name" value="PARANEOPLASTIC ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23095:SF55">
        <property type="entry name" value="PARANEOPLASTIC ANTIGEN MA1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14893">
        <property type="entry name" value="PNMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Paraneoplastic antigen Ma-like C-terminal" evidence="2">
        <location>
            <begin position="44"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8024913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="332" mass="36970" checksum="8D3DB495D33D856A" modified="2023-09-13" version="1">MNQAVQTSTDGPSGQSESIPNGQGDIPLVPQRVYIPVDHRVPIFSGAPVSKNDPSWSEWVSNLKAALRAWGTPEDQQAEFAIRYLGGVAKREVLVLTPQSRASLTEVLRHLEEIYGDNASLQTLMTQFYARQQRQGESARQYALCLQELLQRVEVRFPHALPDKNCTLRDRFIEGLTNNALKLELRKEIRKTPTCTFHDIKREALELEEELAAEPIIGVPSVGLQQTGAKLNVARQEGTTGPDEFAKLRQEMGEVFAAMQKQISDLQSTVAMTMGMRNAGGQGRRPTDRFTDDGKPICRFCNIPGHVERFCRKKAALSQNSEYASQPAVPPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YMB0</accession>
    <name>A0A9Q0YMB0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.49</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_35157</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.49" evidence="1"/>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8025059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YMB0"/>
    <dbReference type="OrthoDB" id="775972at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00303">
        <property type="entry name" value="retropepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09274">
        <property type="entry name" value="RNase_HI_RT_Ty3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.10.10:FF:000002">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 17.6-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.10.10:FF:000007">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 17.6-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.370:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 17.6-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000045">
        <property type="entry name" value="Transposon Tf2-7 polyprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041373">
        <property type="entry name" value="RT_RNaseH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13975">
        <property type="entry name" value="gag-asp_proteas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17917">
        <property type="entry name" value="RT_RNaseH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="9">
        <location>
            <begin position="342"/>
            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8025059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="824" mass="93199" checksum="24CEAAE69F42E0B4" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YPU7</accession>
    <name>A0A9Q0YPU7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Homologous recombination OB-fold protein OB-fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_35517</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8025335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YPU7"/>
    <dbReference type="OrthoDB" id="21443at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000725">
        <property type="term" value="P:recombinational repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028045">
        <property type="entry name" value="HROB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058570">
        <property type="entry name" value="HROB_OB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14523:SF1">
        <property type="entry name" value="HOMOLOGOUS RECOMBINATION OB-FOLD PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14523">
        <property type="entry name" value="UNCHARACTERIZED PROTEIN C17ORF53 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15072">
        <property type="entry name" value="HROB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homologous recombination OB-fold protein OB-fold" evidence="2">
        <location>
            <begin position="640"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="75"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="150"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="200"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="267"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="347"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="412"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="532"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="723"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="797"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="831"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="172"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="274"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="296"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="307"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="577"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="723"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="831"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="843"/>
            <end position="852"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF15072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8025335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="882" mass="95843" checksum="FFD8B11FF6DCBA31" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YQS9</accession>
    <name>A0A9Q0YQS9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_36039</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YQS9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="163"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8023566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="339" mass="40473" checksum="416B1294A74C8998" modified="2023-09-13" version="1">MRIFSSRMRDLYAGDTFGSHLSTGKMFTQLRNETRRNALVYFTIIPSFELIIKSIYTFFSNFEDKSYDDWSVRFDIFATELKASKERSYFVKYMHEEIAKSFKTIKDHCQIPYTQWIRRPDKSHRMKERWEMTQSVFRKVFWPLVGFSNFLVINYFNVCNSICVEVIFTTFVTFLEWLAWTQCQRFFNDYVDTEYEEDEVEHMRETIEIVSSALGKFMDGFYTVVNFFTTIGGEIIRLMKLGRAKDQELRTHFEVFKEKAKDIKRSCRNIIGIVPNIKSDFVAIPTEDKERLYVHKRVQEIKNSFLHMNPEMSNVTAFVLDKCSLTNFSFEHSHVNVEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1B932</accession>
    <name>A0A9Q1B932_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Zinc finger CCHC domain-containing protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_43294</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000262">
        <property type="protein sequence ID" value="KAJ8018621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1B932"/>
    <dbReference type="OrthoDB" id="3863715at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002218">
        <property type="term" value="P:activation of innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042509">
        <property type="entry name" value="ZCCHC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22639">
        <property type="entry name" value="GAG-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22639:SF3">
        <property type="entry name" value="ZINC FINGER CCHC DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="102"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="120"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="251"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="326"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="277"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="326"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="354"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="384"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="411"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8018621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="527" mass="59142" checksum="12C637D66F17649B" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1B9K6</accession>
    <name>A0A9Q1B9K6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">von Willebrand factor D and EGF domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_41246</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8019596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="382013at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000020">
        <property type="entry name" value="Latent-transforming growth factor beta-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050969">
        <property type="entry name" value="Dev_Signal_Modulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013032">
        <property type="entry name" value="EGF-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013111">
        <property type="entry name" value="EGF_extracell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058727">
        <property type="entry name" value="Helical_Vwde"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057885">
        <property type="entry name" value="Ig_VWDE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001846">
        <property type="entry name" value="VWF_type-D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14949">
        <property type="entry name" value="EGF-LIKE-DOMAIN, MULTIPLE 7, 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14949:SF51">
        <property type="entry name" value="VON WILLEBRAND FACTOR D AND EGF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07974">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25024">
        <property type="entry name" value="EGF_TEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12661">
        <property type="entry name" value="hEGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25776">
        <property type="entry name" value="Ig_VWDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00094">
        <property type="entry name" value="VWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26129">
        <property type="entry name" value="Vwde"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00216">
        <property type="entry name" value="VWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51233">
        <property type="entry name" value="VWFD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1 5" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="VWFD" evidence="8">
        <location>
            <begin position="418"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="7">
        <location>
            <begin position="1184"/>
            <end position="1220"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="7">
        <location>
            <begin position="1222"/>
            <end position="1256"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="7">
        <location>
            <begin position="1329"/>
            <end position="1361"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="7">
        <location>
            <begin position="1362"/>
            <end position="1394"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="7">
        <location>
            <begin position="1395"/>
            <end position="1426"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="7">
        <location>
            <begin position="1458"/>
            <end position="1490"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="7">
        <location>
            <begin position="1491"/>
            <end position="1522"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="7">
        <location>
            <begin position="1554"/>
            <end position="1586"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="733"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="733"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="1210"/>
            <end position="1219"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="1246"/>
            <end position="1255"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="1333"/>
            <end position="1343"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="1351"/>
            <end position="1360"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="1366"/>
            <end position="1376"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="1384"/>
            <end position="1393"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="1398"/>
            <end position="1408"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="1416"/>
            <end position="1425"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="1462"/>
            <end position="1472"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="1480"/>
            <end position="1489"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="1494"/>
            <end position="1504"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="1512"/>
            <end position="1521"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="1558"/>
            <end position="1568"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="1576"/>
            <end position="1585"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8019596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1597" mass="177089" checksum="9639807B31FBB6B6" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BAR2</accession>
    <name>A0A9Q1BAR2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_44681</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010001108">
        <property type="protein sequence ID" value="KAJ8017717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BAR2"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8017717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="70" mass="7652" checksum="B82D228813D0C561" modified="2023-09-13" version="1">MDFALPQNHESDSDSSDDGDIENAPLNEVPIPKEDMWDDFDAEKVERHASGLVAGLLASNSRAHSLSLML</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BB58</accession>
    <name>A0A9Q1BB58_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GIY-YIG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_39737</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BB58"/>
    <dbReference type="OrthoDB" id="10055412at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8020210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="155" mass="18060" checksum="C167AA4FB98B7716" modified="2023-09-13" version="1">MPNMKSIVDAHNKKIMKAQMPSPETNPCNCRNENDCPLDGKCRTANVVYQATVKSNDREETYVGLTENTFKLRLANHQQSFTKEKYRNQTELSKYVWTLTNSNTDFKIHWKILAHAPSYSNVSKRCNLCMMEKFYSICYPEMASLNQRSELVNEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BCX0</accession>
    <name>A0A9Q1BCX0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Cytochrome P450 2U1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_40496</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BCX0"/>
    <dbReference type="OrthoDB" id="2789670at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002403">
        <property type="entry name" value="Cyt_P450_E_grp-IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050182">
        <property type="entry name" value="Cytochrome_P450_fam2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300">
        <property type="entry name" value="CYTOCHROME P450 508A4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00465">
        <property type="entry name" value="EP450IV"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0349">Heme</keyword>
    <keyword evidence="3 4 5" id="KW-0408">Iron</keyword>
    <keyword evidence="2 4 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="4">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR602403-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8020808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="69" mass="7936" checksum="754030D7B2FA48FE" modified="2023-09-13" version="1">MHHDPKLYPDPEKFDPNRFLTEDGQVKKQESFMPFGAGRRVCMGESLAKLELSVPFLCWDAPSVFLFCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BD73</accession>
    <name>A0A9Q1BD73_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Cytochrome P450 2J5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_40942</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8021153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BD73"/>
    <dbReference type="OrthoDB" id="2789670at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000036">
        <property type="entry name" value="CYtochrome P450 family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050182">
        <property type="entry name" value="Cytochrome_P450_fam2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300">
        <property type="entry name" value="CYTOCHROME P450 508A4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300:SF417">
        <property type="entry name" value="CYTOCHROME P450 508B1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0349">Heme</keyword>
    <keyword evidence="5 7 8" id="KW-0408">Iron</keyword>
    <keyword evidence="3 7 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 8" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040162026" evidence="9">
        <location>
            <begin position="19"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="7">
        <location>
            <position position="429"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8021153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="492" mass="55228" checksum="90F32F85302CEC91" modified="2023-09-13" version="1">MIAIVLTSIFVWAFLILGQDRNLNIPPGPSGWPLIGNLPAFLSSNPTAFFAECSKKFGDICKLQLGHQRVFIVSKIDLVRKVFTNRNAQNRIPNAAYYKVFGQNAHGVVFASNSPWKDARKFILRVFRDLGVGRGPFESKIVSEAERLVVKLGEGVDKRELDLKQKFFWAVSNVTCGIVFGKQYEYDDPTLKLFVDFVHTLFKSAGPGAFFATSRFLCALPFGPGSKVVNCKRKFKLKMDKIINEHISSFDPNQSPSNVLDAFLLEIHENAGNSVHINLENLQACFVDLFVAGTETTASTLSFAILFLATHLHVQENCHQELDLFLKEGGQLKYSERFKLPFVQAVIHETQRISNVLPLGLPHLTEKDIKLSGFDLPKGSAIAANMKVIFMNDSIFENPDEFNPARFIKDGIFEESSHVIPFSTGSRVCLGERLARMQLFIFLSHLLYRYSFKPPKDVVLPRTLDGVEDAVARSPLPYPVVITERFPSNTSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BDG9</accession>
    <name>A0A9Q1BDG9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Translocon-associated protein subunit gamma</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_38573</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BDG9"/>
    <dbReference type="OrthoDB" id="10059529at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009779">
        <property type="entry name" value="SSR3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07074">
        <property type="entry name" value="TRAP-gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8021393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="61" mass="6731" checksum="CE41915877B89F5D" modified="2023-09-13" version="1">MPSQGGKKFTREEELLLQDFSRNMSTKSSALFYGNALIVAALPASLKTCETHISETNSHRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BF79</accession>
    <name>A0A9Q1BF79_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Core histone macro-H2A.1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_37357</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BF79"/>
    <dbReference type="OrthoDB" id="9421954at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00074">
        <property type="entry name" value="HFD_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02904">
        <property type="entry name" value="Macro_H2A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000013">
        <property type="entry name" value="Core histone macro-H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.220.10:FF:000002">
        <property type="entry name" value="Core histone macro-H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.220.10">
        <property type="entry name" value="Leucine Aminopeptidase, subunit E, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002119">
        <property type="entry name" value="Histone_H2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032454">
        <property type="entry name" value="Histone_H2A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002589">
        <property type="entry name" value="Macro_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043472">
        <property type="entry name" value="Macro_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035796">
        <property type="entry name" value="Macro_H2A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23430">
        <property type="entry name" value="HISTONE H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16211">
        <property type="entry name" value="Histone_H2A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01661">
        <property type="entry name" value="Macro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00620">
        <property type="entry name" value="HISTONEH2A"/>
    </dbReference>
    <dbReference type="SMART" id="SM00506">
        <property type="entry name" value="A1pp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00414">
        <property type="entry name" value="H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52949">
        <property type="entry name" value="Macro domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51154">
        <property type="entry name" value="MACRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="9" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Macro" evidence="10">
        <location>
            <begin position="174"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8022457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="360" mass="38714" checksum="B1CD7BBB5B27E6D8" modified="2023-09-13" version="1">MSARGGKKRVKFVSKSTRAGVLFPVGRMHRYLKKVSVKYRIGAGAPVYLAAVIEYLTAEILELAGNAARDNRRARITPRHILLAIANDMELHMLLKNVTIPSGGVLPHIYPELLTKKSSKGKPVFDHPNQNVAPPKVRTVAAKKQLPPKKLLTKALAKGNIGGSPAKGKAGAKTVLSEKKLFLGQKLTVIQGDISDVTVDCVVNPTNGSFFLGGEVGSALERAGGKEFTDEVDKLYKAHGSLELAGAAICPGHKFPAKFVIHCHSPTWGSASAIPNLEKCIKNCLALADEKNLNTIAIPAVSSGKAGMPKQTAAETVLRAIKNYFVSVMASSLKQVYFVLYDKESVNVYISELSKLDVDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BFI7</accession>
    <name>A0A9Q1BFI7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_33572</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BFI7"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8025881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="79" mass="9070" checksum="379DF1D88218F06B" modified="2023-09-13" version="1">MQYCPISQKTPPPQSFLLGSRALKQRILFVSLEADAEFKCANSLMKATFMHSVSTELKDADIRQEMKPSYFMTNFQMKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BHP7</accession>
    <name>A0A9Q1BHP7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Growth/differentiation factor 8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_35342</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the TGF-beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8025202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BHP7"/>
    <dbReference type="OrthoDB" id="5948587at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13751">
        <property type="entry name" value="TGF_beta_GDF8_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001839">
        <property type="entry name" value="TGF-b_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001111">
        <property type="entry name" value="TGF-b_propeptide"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015615">
        <property type="entry name" value="TGF-beta-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017948">
        <property type="entry name" value="TGFb_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848:SF262">
        <property type="entry name" value="LD29161P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848">
        <property type="entry name" value="TGF-BETA FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00019">
        <property type="entry name" value="TGF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00688">
        <property type="entry name" value="TGFb_propeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00204">
        <property type="entry name" value="TGFB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00250">
        <property type="entry name" value="TGF_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51362">
        <property type="entry name" value="TGF_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 6" id="KW-0339">Growth factor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040239460" evidence="8">
        <location>
            <begin position="24"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="TGF-beta family profile" evidence="9">
        <location>
            <begin position="326"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="40"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="40"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="62"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="73"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8025202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="442" mass="50957" checksum="AB9EDE389C42254D" modified="2023-09-13" version="1">MNLCNRATVICFVFLCLLHKILAHGNHRAMNSIANAVDNPPDRTIHHEGNKNPLYRARSPPADEDGYGEEVSSESTRTTPTLQLHTETHETSECPMCYLRREEARLLRIEYVKRTILEKLQMTSPPNNSNIVLPKNAEITRFMNQYFNEQRTASMQRDEPRSSLEPSTRLEKMILFPKKPPSDWDVTENTSYAFTFSEKLFNYDIKDASLHVHIRARRSSQGNSIHYLELRRVHHPPSGSNQPKIRYTSTKKIMLHPPKGQWVVLNMTTMAQEWIDYPEDHNVIQIALIGEDGSSQDALLDTNIDSTFRKQRSFLMMNIREKRHPRYRRAVDEAMVCTARSPVTTCCKYPMEIDFEEMGWDWVIAPRRYDANYCHGDCPRQYMLQYAHTQIIDAIGHSTLGATGPCCAPRTMSGLSMLHTDRDDNIQYTVIDSMTVDSCHCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BL71</accession>
    <name>A0A9Q1BL71_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Lysosome membrane protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_30906</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CD36 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BL71"/>
    <dbReference type="OrthoDB" id="18585at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005044">
        <property type="term" value="F:scavenger receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002159">
        <property type="entry name" value="CD36_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005429">
        <property type="entry name" value="LimpII"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11923:SF51">
        <property type="entry name" value="LYSOSOME MEMBRANE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11923">
        <property type="entry name" value="SCAVENGER RECEPTOR CLASS B TYPE-1 SR-B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01130">
        <property type="entry name" value="CD36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01609">
        <property type="entry name" value="CD36FAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01611">
        <property type="entry name" value="LIMPII"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8028614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="407" mass="46401" checksum="458E0CD4BE8F4CE5" modified="2023-09-13" version="1">MARFVCVILSLVASLIFLIIGIAVLVGYPIIYRHLTLENVVLANKNMGFNLWKQPPYPIYFQAWVWNVTNSENVTTKGEKPYLVQRGPYTYREIRNKEDVDFNTDDEYISYISPQIFEFVPEMSVGDPKTENITTINIPYLTVANSASFMTSLEQLLIQIAARRVHSKVYDTFRVFDLLWGYTDPLLEELHKEKPDLVPTTFFGLFMDKNNTDDGVYEVNSGKTDASKTAVITSFRNRTNLNYWTTDTCNMINGTDGTYYPPFLNKNKPLYIFSSDICRSIKATFEKTIKVLGVPAHRYVGPPEVFQNATENPANIGFCTPNEKHCLPGGLLNVSNCHEDAPVVMSLPHFLYSEGSVLDMVQGEINPVKEEHQTAFDLEPNTGAPMQVFKRLQINVYQKSYPFFVHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BLF2</accession>
    <name>A0A9Q1BLF2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Aromatic-L-amino-acid decarboxylase</fullName>
            <ecNumber evidence="8">4.1.1.28</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">DOPA decarboxylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_31058</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 11 12">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the group II decarboxylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.28" evidence="8"/>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BLF2"/>
    <dbReference type="OrthoDB" id="639767at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004058">
        <property type="term" value="F:aromatic-L-amino-acid decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042423">
        <property type="term" value="P:catecholamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042427">
        <property type="term" value="P:serotonin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06450">
        <property type="entry name" value="DOPA_deC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1340.10:FF:000001">
        <property type="entry name" value="Histidine decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000025">
        <property type="entry name" value="Histidine decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1340.10">
        <property type="entry name" value="dopa decarboxylase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010977">
        <property type="entry name" value="Aromatic_deC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002129">
        <property type="entry name" value="PyrdxlP-dep_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11999:SF167">
        <property type="entry name" value="AROMATIC-L-AMINO-ACID DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11999">
        <property type="entry name" value="GROUP II PYRIDOXAL-5-PHOSPHATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00282">
        <property type="entry name" value="Pyridoxal_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00800">
        <property type="entry name" value="YHDCRBOXLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0127">Catecholamine biosynthesis</keyword>
    <keyword evidence="5" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="7 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="6 11 12" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="11">
        <location>
            <position position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00040968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00041275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR602129-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8028734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="478" mass="54362" checksum="8CBD43236B38C7AD" modified="2023-09-13" version="1">MATSEEFRIAGKDMIDYVIDYINTCGERPPLAKVTPGYLQDLIPLEAPEKPESWDDIKKDIERVIMPGVTHWNSPHFHAFFPAGSSYPSILGDILMDATTNIGFSWIAGPACTELEMIVLNWLVRMMKLPHVFLNEKGSPGGGSMQGSSSESTLYSLLAAKMRMIRRLKELNSDLDQFDVLPKFIAYISDQTLFCVEKACMIASVRRRILPTDENFILRGNVLRAAIEKDKDDGMIPLFVCATLGTTHSLAFDPLEELGPICLEHDVWLHVDAAYAGTAFICPEYRHFLNGVEYVTSLNISPHKLMRVHIDLSILWVKSRADIEKAFFVDPAIMRHEHQEDTIDYRHWQIPVGRKFRSLKLWFVLRMFGQENLQRHVRNQAQLAKEFRDLMASDPRFTIIAEVVLGLVCFRLKGSDEQNKELLDCVNKSGDIFITGTQLKEQYVLRFVVCAASTESKHIRFAWKAIEGAAGKLLQKEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BLG1</accession>
    <name>A0A9Q1BLG1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_31080</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BLG1"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8028749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="120" mass="13343" checksum="C66A4C768EC6A3DF" modified="2023-09-13" version="1">MYTWHSNIDRAIHCRLDFFVVSRHLRTSVLNSSIISLIGSDHSGVSLELQIGVARGKGTWKLNTALLDDPTYIETIKSAIHSTVNSCRDKDPSFVWEACKLSIRSASIAYSSTFQSLAAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BLY8</accession>
    <name>A0A9Q1BLY8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase A2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_28166</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8028909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BLY8"/>
    <dbReference type="OrthoDB" id="10060349at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="301"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="305"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8028909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="342" mass="37584" checksum="047EAC049F7E25C8" modified="2023-09-13" version="1">MAGCSMVAEEKLEQVDDCCNKSLHSKSNVSGKRAYGKLPQTTLQDTQHETMEKSCEESPGETGGRPRVGTIRKGRLVSVYLEGSTQEETIKFLVDSGSSMSFLSKKKYLQLVPDKDEDLKPTGVPQQLADGSPLEVVGQVELVVKLEGSTLNVEFQVAELDLDGILGPDFLHSHQCQWDWVNCSMVVAGVAVPLIRGMKYDEPIVSKMQAKDNFVVPAGHEMLVFGEVPETQVSKRVAEIRPKGKFVERTGLLVACAMMKPGSSRVPICLMNPGTKDVKVWKKTCMAEFYELDEADDILGTISPHQEKDDPVEDATRKNGGVFPDCEGGVAKERYQTEEAVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BMG6</accession>
    <name>A0A9Q1BMG6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Activated RNA polymerase II transcriptional coactivator p15</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_28838</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transcriptional coactivator PC4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BMG6"/>
    <dbReference type="OrthoDB" id="2505440at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060261">
        <property type="term" value="P:positive regulation of transcription initiation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.31.10">
        <property type="entry name" value="Transcriptional Coactivator Pc4, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003173">
        <property type="entry name" value="PC4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009044">
        <property type="entry name" value="ssDNA-bd_transcriptional_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045125">
        <property type="entry name" value="Sub1/Tcp4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13215">
        <property type="entry name" value="RNA POLYMERASE II TRANSCRIPTIONAL COACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02229">
        <property type="entry name" value="PC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54447">
        <property type="entry name" value="ssDNA-binding transcriptional regulator domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcriptional coactivator p15 (PC4) C-terminal" evidence="8">
        <location>
            <begin position="55"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="30"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8029448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="117" mass="13428" checksum="7C6263C3E9416F2E" modified="2023-09-13" version="1">MPKHKSAATIDSSGSSSDSGPDPEPEPAPKKAKKEKKEKKKKQEDDDDEKEHMYALSRQRFCNVREFRGKILIDIREYYEADGEMKPGKKGISLTIEQWRKLESKMDEINESIQALS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BN10</accession>
    <name>A0A9Q1BN10_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_29128</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BN10"/>
    <dbReference type="OrthoDB" id="6118021at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45823">
        <property type="entry name" value="T-SNARE COILED-COIL HOMOLOGY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45823:SF1">
        <property type="entry name" value="T-SNARE COILED-COIL HOMOLOGY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="219"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="269"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="452"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="576"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="219"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8029677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="762" mass="85202" checksum="1D9A66898F2C1BD9" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BNJ6</accession>
    <name>A0A9Q1BNJ6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_29350</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BNJ6"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8029843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="53" mass="5808" checksum="A531581B9983A47A" modified="2023-09-13" version="1">MLPDVLGVSIPQHYIDSKPPDEPWKPPVPTSEMECSDQGTECTKDGSQIEHTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BQ53</accession>
    <name>A0A9Q1BQ53_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_27597</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8031008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BQ53"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8031008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="277" mass="31526" checksum="DD21F4B889FC87EC" modified="2023-09-13" version="1">MLQYFTIFSEVKQLTDLQKGGRMDVLCKIEPERGLQNAQKPGNATLEVWLPTLRQLMGGEEGIGRVLNSIPFGIFFTVFILHLPSYSQATYRKTLVLEQYPSLVKYKQLKFVLLFLISIPLPRKEQSPTVNFDTPCTKNIHWGDDCLSLNWSAPGCFRPGVLPTSTAVYKTDFDGYADRPMLLQGYTGALIPPTFILTPKPEATILDKIIWDKSPRPTFNVGFEGGQTILDKIIWDKSPRPTFNVGFEVVYLPFVTLPYFSSRVSLWDRRAVAWEHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BQ61</accession>
    <name>A0A9Q1BQ61_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Carbohydrate sulfotransferase 15</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_27257</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BQ61"/>
    <dbReference type="OrthoDB" id="8068875at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050659">
        <property type="term" value="F:N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019319">
        <property type="term" value="P:hexose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052654">
        <property type="entry name" value="CS_Sulfotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15723">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15723:SF0">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="domain" description="Sulfotransferase" evidence="3">
        <location>
            <begin position="464"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="91"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8030761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="645" mass="75258" checksum="159DE80D6B3B4882" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BQP5</accession>
    <name>A0A9Q1BQP5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_27399</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BQP5"/>
    <dbReference type="OrthoDB" id="10683372at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="333"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="397"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="44"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="397"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8030861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="443" mass="48130" checksum="853B665C1F4E6104" modified="2023-09-13" version="1">MLPCTAAPRQSRVVLGELIIPQPRPRIGGKQSTLNMFFKPVPVSPSSSTSILNSSTDKESSHTFTKTSSQLNPGGDTTEPLLESMCNVLPESLAGGDSEESFGIIKENNICDMGIRSENCEENAVDGFTGCTVDENSSGTATCNTGHSSKEDNSEASVIGKCSYVFHKVDLTDGLGARKRPHGLIESAGKSFLVQDVPSKRYRTESYSTQEDVIETSNFGNCRRDNRSETKGNYLSGKGNTDNSFKVSCLPAKCESVNSESVVNKPGKSLKINRTLTSQSDLLKTTSGAKVHDKNSLISQNDVCSDMMDDSLQSISEKFVNRKSTFASQSNRLKDLTEANDDDKSDLKSQNYAPLEMDNSLQSSASFEDSLSADDSLTPAILRNSIYRELNLTSDLPLTNAQPSHGKETSSCNSDEIPDTVAFDSLHDWSVSENYSTETTKKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BRW2</accession>
    <name>A0A9Q1BRW2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Nose resistant to fluoxetine protein 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_24759</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BRW2"/>
    <dbReference type="OrthoDB" id="10006435at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052728">
        <property type="entry name" value="O2_lipid_transport_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11161">
        <property type="entry name" value="O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11161:SF0">
        <property type="entry name" value="O-ACYLTRANSFERASE LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040165592" evidence="2">
        <location>
            <begin position="16"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="125"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="154"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="193"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8031539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="213" mass="24059" checksum="924539366D7E5E87" modified="2023-09-13" version="1">MLLLYMFIMPHLVWGPRAPFFADQHKCKNYWWTTLLFINNIYPAMNETCMAWTWYLSNDMQYFVISPVFIYAFCRNSLVALGLLSLFLSICMGATVLLVACVNYTLPVWIAGRKPVTDLIYRAPFVRISPYLVGLVAGFVLFKIKASKPTLPRTAFFLGWLLASGVTMTILFGTNVQKPMGIVGSVLFETFSHFSWGICLTWVVTACEFGGPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BRY9</accession>
    <name>A0A9Q1BRY9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ZU5 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_24821</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BRY9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.220.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000906">
        <property type="entry name" value="ZU5_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00791">
        <property type="entry name" value="ZU5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51145">
        <property type="entry name" value="ZU5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ZU5" evidence="1">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8031584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="85" mass="9544" checksum="EA86CFAAB179AAE6" modified="2023-09-13" version="1">MLEIPSTGVILAVPSNALSQHMEKCEIHMRIIPRKMLNEQVASFSLNSATVVELLPAGLSFQRPVRLSLPHCLVLQQTRERKARI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BUB9</accession>
    <name>A0A9Q1BUB9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">N-terminal E2 ubiquitin-conjugating enzyme</fullName>
            <ecNumber evidence="2">2.3.2.23</ecNumber>
            <ecNumber evidence="6">2.3.2.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_23537</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.</text>
            <dbReference type="EC" id="2.3.2.23"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-N-terminal-amino acid = [E1 ubiquitin-activating enzyme]-L-cysteine + N-terminal-ubiquitinyl-[acceptor protein].</text>
            <dbReference type="EC" id="2.3.2.25"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="2"/>
    <dbReference type="EC" id="2.3.2.25" evidence="6"/>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BUB9"/>
    <dbReference type="OrthoDB" id="406833at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd23808">
        <property type="entry name" value="UBCc_UBE2W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000022">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="8">
        <location>
            <begin position="6"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00042168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8033333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="154" mass="17734" checksum="AC68DB9172ABF1E6" modified="2023-09-13" version="1">MFSNTAMQKRLQKELMQFQQEPPKGMTIDTESVSNTLTTWIVHIEGAEGSLYHGEKFQLQFRFSNRYPFDSPEVMFVGSEIPVHPHIYSNGHICLSILTDDWSPALSVQSVCLSILSMLSSCTEKKHPPDNSFYVRTCNKSPKKTKWWYHDDSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BVN8</accession>
    <name>A0A9Q1BVN8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_24032</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BVN8"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8033701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="54" mass="6334" checksum="DA067B315F81CE81" modified="2023-09-13" version="1">MWMHYGERKNPIVFGGGQRSFGVTRGQNLKTLFTQYLKVGSLDRFHTWCVDALW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BW11</accession>
    <name>A0A9Q1BW11_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Tyrosine-protein kinase</fullName>
            <ecNumber evidence="11">2.7.10.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HOLleu_24167</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Nanhai2018</strain>
            <tissue evidence="15">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein kinase superfamily. Tyr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.2" evidence="11"/>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BW11"/>
    <dbReference type="OrthoDB" id="5984265at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004715">
        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048468">
        <property type="term" value="P:cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050793">
        <property type="term" value="P:regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00192">
        <property type="entry name" value="PTKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00173">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001512">
        <property type="entry name" value="Receptor tyrosine-protein kinase erbB-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050198">
        <property type="entry name" value="Non-receptor_tyrosine_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24418">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 11 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="2 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 11" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="SH2" evidence="13">
        <location>
            <begin position="79"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="208"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="24"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00051245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ8033805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="482" mass="54533" checksum="A2FF016C5746E545" modified="2023-09-13" version="1">MGCCRSKDDSRTIGGIERYPTEGGLNSRSASETASRHSHVPFTTSFTDINNSQFSLTGASNQPSRIEEQDIIPLIREYWYFGKIDIDRCTKVLGDGYVPDGTFIVRESTSSSGTLCISVKGRSPKGPGVFSVRVTESKDRFGYKLLRSGVDNKVFTSIRNVIRYYQDNGIHSNGLSISLGEGLKNKHYYLGEDEEEDRSFQIKSEDIDVQSEQLGKGHFGIVYKGVWKKRNGQQVLVAVKKLIESDKEELEKFITEANVMKMVYHDNVVTFVGVYFEENQNPVIVTELMPKGNLLNYLTANKDTSLGDLYYFAYQVAAGMTFIASKNIIHRDLAARNVLLGEQHIVKIADFGLSRVLKQDEIYSSSIAEFPVKWTAPEGLAPPPRYFSTKSDVWSYGIFLSELITRGEEPYKSVPHISITKDRLIHQGYRMQKENLGEDCSLGLYAIMEKCWQSDPQQRPDFKTVAADIRRLYKPHLLLQQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BWD0</accession>
    <name>A0A9Q1BWD0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_24517</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8034091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BWD0"/>
    <dbReference type="OrthoDB" id="419189at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33332">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8034091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="321" mass="36123" checksum="9B5634C4819CA4AD" modified="2023-09-13" version="1">MLYADDTQLYMVCDKPSEVTNAVEECLIEICTWMKSNLLVLNSDKTEVVHFCSRHKKVVKELTSLKIGQSIITPSNSVRNLGVHFECSGNFNVHINHICKSAYFALHRIGKLRTLLDQAATEKLVHAFVTSRLDYCNSLLYGCPEFHLDKLQSIQNAAARLVKRQKKSEHITPVLYELHWLGVHGRPLTHISHKCIPPCKFPARLLVVLEKGQFEQNVLQSLMHPSPTIIHPSRCGSSACVVDDDRADRDNEGVYFAGDARGGRKTEILSCGTLGFGGYYVALLENPNVKKCFGDKLVPVRCILPQFMRPFHTQVYAVSDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BXF6</accession>
    <name>A0A9Q1BXF6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">WW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_22258</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BXF6"/>
    <dbReference type="OrthoDB" id="187617at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WW" evidence="1">
        <location>
            <begin position="424"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8035133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="589" mass="67767" checksum="C7EBF5EFC054FB73" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BYC7</accession>
    <name>A0A9Q1BYC7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_21777</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BYC7"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8034789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="82" mass="9211" checksum="FC2209239B24BC56" modified="2023-09-13" version="1">MFSMEVLFCSTHTVVLLLRILIVVTWRRGKQTSTDNTCSLGATCSYRTHGLLKNAQRQSSPTYPNFVSVHNYVNPAVVVTYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BZD2</accession>
    <name>A0A9Q1BZD2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">LRP chaperone MESD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_19298</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MESD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8035575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BZD2"/>
    <dbReference type="OrthoDB" id="75833at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.260:FF:000031">
        <property type="entry name" value="LDLR chaperone MESD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.640">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019330">
        <property type="entry name" value="MESD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17600:SF2">
        <property type="entry name" value="LRP CHAPERONE MESD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17600">
        <property type="entry name" value="MESODERM DEVELOPMENT CANDIDATE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10185">
        <property type="entry name" value="Mesd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="35"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="199"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="35"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="69"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="83"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="210"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="222"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8035575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="237" mass="27667" checksum="D6B9A4A294CD565A" modified="2023-09-13" version="1">MGKNLIRFTTGRYTIAYGIGLCILTLLVVATYAKEEKKVDEDSPSEKWKKKDPRDYNDADVERLFQQWEENDDEEPDPDDINPYDRPKPKIDLSKLDPENPESMMKMTKRGTPLMMFVSVSGNPTKEETEKISSRWQEQLFNANFNFKRYVVGDDRLLFHVDDGSWAFDIKNFLIDQERCKDVTIDQQIFYGRGSGRPVDNKAHIVGAEEMEKKKKAEEKKNKKKKSKKKAEKKDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C239</accession>
    <name>A0A9Q1C239_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_17421</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8036785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C239"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8036785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="208" mass="24242" checksum="8776C7614021791A" modified="2023-09-13" version="1">MEVPRWYFHGLGYKRSCYGNSIRERVLQLHGCMNPFCARKTKTACDERSAKKISVHAEEFAVIRLFRVQARRQQLAKEGVKPVGKLSKRSATIGWREVCSVQENCGRKCLPDRWKWHFPGFPEKAWDSQKEIQKRSARHITTQEELRWPSGEFKEEQCLEQPQPWSYKVAWFLPRGADEVFFSFSSSLRKAQGGGSHRGTTQTTWFPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C394</accession>
    <name>A0A9Q1C394_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">SEC14-like protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_18718</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C394"/>
    <dbReference type="OrthoDB" id="30289at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.680">
        <property type="entry name" value="GOLD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011074">
        <property type="entry name" value="CRAL/TRIO_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036598">
        <property type="entry name" value="GOLD_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006797">
        <property type="entry name" value="PRELI/MSF1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051064">
        <property type="entry name" value="SEC14/CRAL-TRIO_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23324:SF66">
        <property type="entry name" value="PROTEIN REAL-TIME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23324">
        <property type="entry name" value="SEC14 RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03765">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04707">
        <property type="entry name" value="PRELI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00180">
        <property type="entry name" value="CRETINALDHBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM01100">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101576">
        <property type="entry name" value="Supernatant protein factor (SPF), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50866">
        <property type="entry name" value="GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50904">
        <property type="entry name" value="PRELI_MSF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRELI/MSF1" evidence="4">
        <location>
            <begin position="3"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="CRAL-TRIO" evidence="2">
        <location>
            <begin position="353"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="3">
        <location>
            <begin position="556"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="206"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="248"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="731"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="209"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8037811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="751" mass="85132" checksum="B0A2BD6003AAA6FE" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C5W7</accession>
    <name>A0A9Q1C5W7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">3-keto-steroid reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_16248</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C5W7"/>
    <dbReference type="OrthoDB" id="9989144at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000253">
        <property type="term" value="F:3-beta-hydroxysteroid 3-dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016125">
        <property type="term" value="P:sterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052834">
        <property type="entry name" value="3KSR/17beta-HSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44442">
        <property type="entry name" value="3-KETO-STEROID REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44442:SF1">
        <property type="entry name" value="3-KETO-STEROID REDUCTASE_17-BETA-HYDROXYSTEROID DEHYDROGENASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8038739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="100" mass="10711" checksum="C980AE6C68396398" modified="2023-09-13" version="1">MNGQIKAWSASRKVVVITGGNSGVGFALAQRLLALEITVTVCLACRNVTKAEKAKSELEEEYKGAKVDILQLDTSSIASVNAAAKELQKRHEMTAACQTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C681</accession>
    <name>A0A9Q1C681_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cubilin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_17073</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="10009301at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
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        <property type="entry name" value="CUB"/>
        <property type="match status" value="5"/>
    </dbReference>
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        <property type="entry name" value="LDLa"/>
        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="Membrane frizzled-related protein"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000005">
        <property type="entry name" value="Procollagen C-endopeptidase enhancer 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="8"/>
    </dbReference>
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        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24251:SF37">
        <property type="entry name" value="CUB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24251">
        <property type="entry name" value="OVOCHYMASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00261">
        <property type="entry name" value="LDLRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040495148" evidence="5">
        <location>
            <begin position="21"/>
            <end position="1145"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="6">
        <location>
            <begin position="180"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="6">
        <location>
            <begin position="304"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="6">
        <location>
            <begin position="433"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="6">
        <location>
            <begin position="529"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="6">
        <location>
            <begin position="654"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="6">
        <location>
            <begin position="762"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="6">
        <location>
            <begin position="882"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="6">
        <location>
            <begin position="1067"/>
            <end position="1127"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="27"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="46"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="65"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="72"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="84"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="123"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="142"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="149"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="161"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="1067"/>
            <end position="1094"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8039377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1145" mass="125608" checksum="ED5417DF5CC76278" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C689</accession>
    <name>A0A9Q1C689_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Collagen alpha-1(XXVII) chain B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_16570</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C689"/>
    <dbReference type="OrthoDB" id="10256829at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005581">
        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050938">
        <property type="entry name" value="Collagen_Structural_Proteins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37456:SF6">
        <property type="entry name" value="COLLAGEN ALPHA-1(XXIII) CHAIN-LIKE ISOFORM X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37456">
        <property type="entry name" value="SI:CH211-266K2.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0176">Collagen</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040190122" evidence="2">
        <location>
            <begin position="20"/>
            <end position="1117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="308"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="503"/>
            <end position="1019"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1034"/>
            <end position="1117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="347"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="362"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="377"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="528"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="564"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="782"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="829"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="924"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="945"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="974"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1068"/>
            <end position="1084"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1099"/>
            <end position="1117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8038993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1117" mass="113411" checksum="2993E91358FE2C8C" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C7C6</accession>
    <name>A0A9Q1C7C6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Ecotropic viral integration site 5-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_16842</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C7C6"/>
    <dbReference type="OrthoDB" id="295078at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Ecotropic viral integration site 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000003">
        <property type="entry name" value="Ecotropic viral integration site 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.750">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF22">
        <property type="entry name" value="RAB-GAP TBC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="5">
        <location>
            <begin position="9"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase" evidence="6">
        <location>
            <begin position="589"/>
            <end position="828"/>
        </location>
    </feature>
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        <location>
            <begin position="547"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
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            <begin position="784"/>
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        </location>
    </feature>
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        <location>
            <begin position="275"/>
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        </location>
    </feature>
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        <location>
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            <end position="369"/>
        </location>
    </feature>
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        <location>
            <begin position="401"/>
            <end position="474"/>
        </location>
    </feature>
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        <location>
            <begin position="555"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="784"/>
            <end position="802"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8039201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="828" mass="95901" checksum="9ECC82E26B429FC4" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C7U8</accession>
    <name>A0A9Q1C7U8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_13714</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8039649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C7U8"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8039649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="50" mass="5743" checksum="6DC672F22B5499FA" modified="2023-09-13" version="1">MEPTAFVLGAQHKQWSRENKPVSLNWKKGCGRLDLNQLVGNWDVKPHLTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C856</accession>
    <name>A0A9Q1C856_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_14385</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C856"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8040165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="160" mass="17956" checksum="A22B749E45DBCBFF" modified="2023-09-13" version="1">MPLRPACRACISPMRVPKLRLRESLANRGLQLFRRCHCIEQFEGWANHGCTNVWLRVNHGLLFASCCRNNARNLVTTEDEDPSNSIPSLKSPKSPNPASSRVVTPSSTELPVSLLSIKLKHHEHPSATAKSDQGRSLGEILKGMQIMTLSKRRTKWHAVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C8S7</accession>
    <name>A0A9Q1C8S7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Sulfotransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_14501</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C8S7"/>
    <dbReference type="OrthoDB" id="10010208at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053259">
        <property type="entry name" value="Golvesin-related_Golgi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005331">
        <property type="entry name" value="Sulfotransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32301">
        <property type="entry name" value="COUNTIN RECEPTOR CNR3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32301:SF6">
        <property type="entry name" value="GOLVESIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03567">
        <property type="entry name" value="Sulfotransfer_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8040265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="397" mass="45312" checksum="2C6CB07F433F0F25" modified="2023-09-13" version="1">MVGSRSKLLTFVLHASVMGMLIWLFVLFLYKDSHAVRHRYFFQPFVTDANQIPEERSLSNDGGRKHRGKTVKEIGNLQESVVQYSSINKDKFPIIKNALRNYFLYVNDSKTASSYKATVLAFVHNQKSGGTTLKRCLSEILEYQNKEPVLVTNANRAAVEEKILNGVSESKPNDVFVGAATFGICENLAPTTCAYFTILRDPYERMISHYMFCRNGGETGLQCHNSTIEEYAISIGSLLFTHITAHMLCKKSCVSPNDDCENGTDGSAWRCERLYSSIYDVRRRSVLGYVIKNLDRFFAVIGLFEEYETSLALFQETFGLPLSDVCNGIHANEGTYPDVQGSNEDIGQQRRRYIDEMKRKLKANDTVRNNIEADVKIYERAREIFYKQKQIYIQTRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C8W2</accession>
    <name>A0A9Q1C8W2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_15245</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C8W2"/>
    <dbReference type="OrthoDB" id="6343311at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8040832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="123" mass="13724" checksum="3C1AA22892A3A276" modified="2023-09-13" version="1">MLRTCVPNSFVTSLKVVPNIAQLLSYKGFDCTSMGPYQDECSSCRVGYYGNSLLRRCVSCPKDSDVCDVCICSGEGFIDCSESNLKAMPSNIPRDVLFLNMDFNNITCVPVYFMEQFSQLHSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CA93</accession>
    <name>A0A9Q1CA93_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 13">Alpha-amylase</fullName>
            <ecNumber evidence="5 13">3.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HOLleu_15289</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Nanhai2018</strain>
            <tissue evidence="17">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>Endohydrolysis of (1-&gt;4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1-&gt;4)-alpha-linked D-glucose units.</text>
            <dbReference type="EC" id="3.2.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>chloride</name>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the glycosyl hydrolase 13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.1" evidence="5 13"/>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CA93"/>
    <dbReference type="OrthoDB" id="550577at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004556">
        <property type="term" value="F:alpha-amylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11317">
        <property type="entry name" value="AmyAc_bac_euk_AmyA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006048">
        <property type="entry name" value="A-amylase/branching_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031319">
        <property type="entry name" value="A-amylase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006046">
        <property type="entry name" value="Alpha_amylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="GH13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43447">
        <property type="entry name" value="ALPHA-AMYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02806">
        <property type="entry name" value="Alpha-amylase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00110">
        <property type="entry name" value="ALPHAAMYLASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00632">
        <property type="entry name" value="Aamy_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="10 13" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="9" id="KW-0868">Chloride</keyword>
    <keyword evidence="11 13" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="7 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040449578" description="Alpha-amylase" evidence="14">
        <location>
            <begin position="16"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 13 catalytic" evidence="16">
        <location>
            <begin position="26"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-amylase C-terminal" evidence="15">
        <location>
            <begin position="404"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ8040864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="493" mass="55040" checksum="B18DAD2ACE9415D0" modified="2023-09-13" version="1">MKVLILLLLFTAVKGQYDLNMAYGRSTMVHLFEWSWDDIASECENFLAPYDYGGVQVSPPSEHYLSWDNVWWDRYQPVSYKLDCKKGDEAAFRNMVTRCNNVGVRIYVDAVINHMAAGGSTSSGSSSYDAGAYSFPAVPYSSWDFNVPNGYCSSPDGGVNDYGNANEVRNCNLLGLTDLSMGKSYVQDKIVEYLNNLISMGVAGFRVDAVKHMWPDHLKSIYDRLDNLDSSIFGFGKRPFFVQEVIDMGYGEPITASEYTYIGRVTEFKYSDKIGTVAKGYDQMKWLVNFGEDWGFMSRDYALVFVDNHDNQRNHGGGGNIVTHTSSKQYKIASTFMLGYDYGVTRVMSSFFFSDNDYGGPSNSPYFTSDGACNVNSGWVCEHRWREIRNMVGFRKTCEGQGVNNWWDNGNNMIAFSRGSEGFIAINGDSYQMSEWLQTGMPAGTYCDVISGDKSGNSCTGNAITVYSDGKAYFEISNSSDSPIIAIHANSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CAK5</accession>
    <name>A0A9Q1CAK5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Mitochondrial calcium uniporter regulator 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_11959</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CCDC90 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CAK5"/>
    <dbReference type="OrthoDB" id="889336at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024461">
        <property type="entry name" value="CCDC90-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14360">
        <property type="entry name" value="PROTEIN FMP32, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14360:SF1">
        <property type="entry name" value="PROTEIN FMP32, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07798">
        <property type="entry name" value="CCDC90-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="101"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8041213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="122" mass="13796" checksum="04F8F51037D4CC9E" modified="2023-09-13" version="1">MSSIQTLKKDMVILEKTEFTLLRNESEKLAFEVEKLKTQLKDEIQKVSNNVTLDMNLERSRSKEATASVVQQIDLSNQMIKTEVANMKAMLEASKTDTLKYFAATLLGCLTLSLGAFRLFKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CDF0</accession>
    <name>A0A9Q1CDF0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Anaphase-promoting complex subunit 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_09789</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8042906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CDF0"/>
    <dbReference type="OrthoDB" id="308440at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045842">
        <property type="term" value="P:positive regulation of mitotic metaphase/anaphase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12558:SF36">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12558">
        <property type="entry name" value="CELL DIVISION CYCLE 16,23,27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14559">
        <property type="entry name" value="TPR_19"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="340"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="520"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="527"/>
            <end position="547"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8042906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="561" mass="63366" checksum="B387D7EED69E448D" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CE25</accession>
    <name>A0A9Q1CE25_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_10238</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CE25"/>
    <dbReference type="OrthoDB" id="6414146at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022894">
        <property type="entry name" value="Oligoribonuclease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11046:SF29">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11046">
        <property type="entry name" value="OLIGORIBONUCLEASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8043240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="374" mass="41920" checksum="32C2A7E96C4C329C" modified="2023-09-13" version="1">MLKGGKNCLHSDATTKWHKHYQGFQMTTPAGKTLSIGMDQVASGSTDALLEAFNNCIEELSSTLSDDSPDEVTVANLISTIKYTMSDQGPINPSFNKQLQDIRAKCLSKEHDNWESLPEKVKEDMVDMGNFFCKMHILVNFATEADKALKIFESHVINDGNNPHTFDSCSESGSVRLIRTACKALTKHGSDKSGVASYWNSFLSTKERVNKLVTFRGNRFNIVFYDAAAVFYHLSDIEEFLTSYPDPNKLLQSVHFDCKQNVFQAGVKALAITDKLITGPMWSCIEAADSILDLNEALHKLQQSLEKFSLDASTLLQGQETFFDDNSVHKDTLFESIFSDDSDDEQLNFMTQMALELILKSFSILLDRQAKDQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CEB2</accession>
    <name>A0A9Q1CEB2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Copper transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_10112</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the copper transporter (Ctr) (TC 1.A.56) family. SLC31A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CEB2"/>
    <dbReference type="OrthoDB" id="73901at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005375">
        <property type="term" value="F:copper ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007274">
        <property type="entry name" value="Cop_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483:SF115">
        <property type="entry name" value="COPPER TRANSPORT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 31 COPPER TRANSPORTERS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04145">
        <property type="entry name" value="Ctr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0186">Copper</keyword>
    <keyword evidence="4" id="KW-0187">Copper transport</keyword>
    <keyword evidence="4" id="KW-0406">Ion transport</keyword>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="27"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="99"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="126"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8043148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="178" mass="20012" checksum="3FE698CF7FE75E76" modified="2023-09-13" version="1">MMNNYFTSLPPHQLFFQSWGSNTPKETVLLLFGLAVLSLLLETLKHLTGTVKLKVLKCIANNSSDTYTCQDDLETTTPANQRTPTSSGVQLELSLSQSFLLNLLLTGIYVVRILIAYLLMLAVMTYNYWILLVVVFSAAVGYYLLPFGAQHTSHIQNEASTKSTEKERKTYRHLESTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CEC9</accession>
    <name>A0A9Q1CEC9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Centromere protein S</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_10080</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TAF9 family. CENP-S/MHF1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CEC9"/>
    <dbReference type="OrthoDB" id="1872155at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071821">
        <property type="term" value="C:FANCM-MHF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031297">
        <property type="term" value="P:replication fork processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000712">
        <property type="term" value="P:resolution of meiotic recombination intermediates"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22919">
        <property type="entry name" value="HFD_CENP-S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029003">
        <property type="entry name" value="CENP-S/Mhf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22980:SF0">
        <property type="entry name" value="CENTROMERE PROTEIN S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22980">
        <property type="entry name" value="CORTISTATIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15630">
        <property type="entry name" value="CENP-S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="104"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="116"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8043119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="125" mass="13982" checksum="96A2C07A1F27DECD" modified="2023-09-13" version="1">MEEQSDGEASDSEKLALDQRFKAAIHYTVGKICTELEEEMEVTFSRHIIAVISEATYKQSGIFASDLEDFCQHAKRTTVTADDVKLLARRSGTLANHISKVHKKMKDAQVASKEKKGQKRKKTGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CG19</accession>
    <name>A0A9Q1CG19_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_11287</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CG19"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="133"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8043959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="336" mass="36425" checksum="E0A09D89B273A7AE" modified="2023-09-13" version="1">MLHAATVVYHILLEIVLGQISRKIMDVTKGLIGRRICGSVEFIVGAIIIVIAALRLEDDYIDIGSFLIGGFVILKGLLGVASKSTGVIIAYLVTSIICTLYFGYSTIASLLVILQYKYWGTRLGLYAHNSVYIILIVSVAVFTIAFIVSVSGVIFSTLPWCGNSQGREMAQQYSDGTAPNGNAGPSSGYNNNAYVYGGYTHLGVHQPQYANVSSDRATSLNNSNTTCINTTQTLQYYRSSTFVGADGLPGYNYINTAYEDPTQNQRCIQGNTSAGAVGQPSYHNSHTAYINTQAQQSVYGNLSADEVSLPRYSYPNHSNTAYVDTNQGPQLYFVNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CGG6</accession>
    <name>A0A9Q1CGG6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase</fullName>
            <ecNumber evidence="3">3.2.2.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_11709</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>NAD(+) + H2O = ADP-D-ribose + nicotinamide + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16301"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57967"/>
            <dbReference type="EC" id="3.2.2.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:16302"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SARM1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.6" evidence="3"/>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8044288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CGG6"/>
    <dbReference type="OrthoDB" id="202764at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003953">
        <property type="term" value="F:NAD+ nucleosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061809">
        <property type="term" value="F:NAD+ nucleosidase activity, cyclic ADP-ribose generating"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035591">
        <property type="term" value="F:signaling adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034128">
        <property type="term" value="P:negative regulation of MyD88-independent toll-like receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048678">
        <property type="term" value="P:response to axon injury"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000043">
        <property type="entry name" value="Sterile alpha and TIR motif-containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039184">
        <property type="entry name" value="SARM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22998:SF1">
        <property type="entry name" value="NAD(+) HYDROLASE SARM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22998">
        <property type="entry name" value="SARM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13676">
        <property type="entry name" value="TIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0391">Immunity</keyword>
    <keyword evidence="5" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="9" id="KW-0520">NAD</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="SAM" evidence="13">
        <location>
            <begin position="84"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="13">
        <location>
            <begin position="155"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="TIR" evidence="12">
        <location>
            <begin position="247"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="406"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ8044288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="428" mass="48626" checksum="34B722EEBCDC3B74" modified="2023-09-13" version="1">MKLIPLLTCHRREAQCLAAFHFAMEASIKKDQNRTKVFNDIGVISHLQRVASSNNDVAAKQASLALRTIGEDVPAKLSFNISCWTVKEVQMWLKNIGFSSFCPAFEKHRVDGDLLITITEEELRSDTEMESSLMRRRFLRELALLKSNTCSGDWELKDWLKNLGPEYPQYTHNLMHCGIDLDLLPFISEDHLSSDAGITNGVHRMKILRAVRPMAVTRDYPRVFDSPSRPKRSILPSDQLDTGSALKNLDVFISYRRATGSLLASLLKVHLQLRNFTVFIDVEKLEAGKFDNNLLNSIRNAHNFVLVLSTAALDRCKGDVEYKDWVHREIVTALESKCNIIPVMDNFKWPKPEELPEDMRAICYFNGIKWIHDYQDACIDKLERFLRGEENMKQYLEGTVIGIKGTQSGHGLRNSRGNSHDSNGGSTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CHB7</accession>
    <name>A0A9Q1CHB7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">S-adenosylmethionine-dependent methyltransferase CRG1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_07855</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CHB7"/>
    <dbReference type="OrthoDB" id="506498at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051052">
        <property type="entry name" value="Diverse_substrate_MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44942">
        <property type="entry name" value="METHYLTRANSF_11 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44942:SF4">
        <property type="entry name" value="METHYLTRANSFERASE TYPE 11 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="4">
        <location>
            <begin position="52"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8044950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="249" mass="28110" checksum="D42AD36F65920FF4" modified="2023-09-13" version="1">MATHQDDKQLFQGESHASAYKQYRPRYEKTDLVEKIMTFLQEKLDPPYDCALDLGCGSGQSSQLLLPHFTKVIGVDCSEAQVKEALNTPFLKNISFRCGDEKDLGFIPDNSLEVVACGQSIHWMDLGTLYPEIDRILKPGGCLAAYGYVTRTVHVIDGESALEGQLQSLYKEIDDWIDPYYSKSIDVLLGGYYDLPLPYSDQKRFSNMKFYAEVTVDSLIGLVQSFSAFQIAIKQSPEKSNILDEYKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CHS3</accession>
    <name>A0A9Q1CHS3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">exodeoxyribonuclease III</fullName>
            <ecNumber evidence="3">3.1.11.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_08658</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.</text>
            <dbReference type="EC" id="3.1.11.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="8">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="8">Probably binds two magnesium or manganese ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA repair enzymes AP/ExoA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.11.2" evidence="3"/>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CHS3"/>
    <dbReference type="OrthoDB" id="6244150at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003906">
        <property type="term" value="F:DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008311">
        <property type="term" value="F:double-stranded DNA 3'-5' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09076">
        <property type="entry name" value="L1-EN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004808">
        <property type="entry name" value="AP_endonuc_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748">
        <property type="entry name" value="AP ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748:SF26">
        <property type="entry name" value="ENDONUCLEASE_EXONUCLEASE_PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0464">Manganese</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="10">
        <location>
            <begin position="11"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="114"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="7">
        <location>
            <position position="144"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="233"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="9">
        <location>
            <position position="146"/>
        </location>
    </feature>
    <feature type="site" description="Important for catalytic activity" evidence="9">
        <location>
            <position position="208"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA substrate" evidence="9">
        <location>
            <position position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR604808-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR604808-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR604808-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8045617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="284" mass="33081" checksum="F6A7ACA95CE3A343" modified="2023-09-13" version="1">MVSITNLKICSVNVRGVRDRHKRRKVFLWLRQKKCDIYFLQETHSVEATEHIWNNEWGNKILFCHGQSNSMGVCILFKRNISIEICNCFSDNIGRGIIANVTITDYNLTLVNIYAPNTDNINFYHSLRDNIENHGFEPFVIGGDFNTVLNVDLDKRGSPLQPHPKCRNEIYSLMKDFDLKDIWRELSHNNFRYTWKSNSDPIIYCWMDYFLISFSLVNTVKSCEMLADLISDHNPITLSFESCFSGRGPGYWKLNVSLLNNADYVQAMKDVVSHFLIPWISMQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CI73</accession>
    <name>A0A9Q1CI73_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Neuronal acetylcholine receptor subunit alpha-9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_08099</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CI73"/>
    <dbReference type="OrthoDB" id="5809364at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005230">
        <property type="term" value="F:extracellular ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18989">
        <property type="entry name" value="LGIC_ECD_cation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19051">
        <property type="entry name" value="LGIC_TM_cation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000028">
        <property type="entry name" value="AcetylCholine Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="236"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="313"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="500"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="6">
        <location>
            <begin position="24"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="7">
        <location>
            <begin position="242"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8045155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="536" mass="61725" checksum="956D7E73F11BD888" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CIY0</accession>
    <name>A0A9Q1CIY0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Inter-alpha-trypsin inhibitor heavy chain H4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_04816</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CIY0"/>
    <dbReference type="OrthoDB" id="299997at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050934">
        <property type="entry name" value="ITIH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013694">
        <property type="entry name" value="VIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10338">
        <property type="entry name" value="INTER-ALPHA-TRYPSIN INHIBITOR HEAVY CHAIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10338:SF108">
        <property type="entry name" value="INTER-ALPHA-TRYPSIN INHIBITOR HEAVY CHAIN H4-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08487">
        <property type="entry name" value="VIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13768">
        <property type="entry name" value="VWA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00609">
        <property type="entry name" value="VIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51468">
        <property type="entry name" value="VIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040483134" evidence="2">
        <location>
            <begin position="20"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="domain" description="VIT" evidence="4">
        <location>
            <begin position="39"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="3">
        <location>
            <begin position="296"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="126"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8046207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="682" mass="76276" checksum="C15B656E47907457" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CJ38</accession>
    <name>A0A9Q1CJ38_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_04812</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CJ38"/>
    <dbReference type="OrthoDB" id="10037236at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8046203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="76" mass="8631" checksum="B716C15B7E39CDBC" modified="2023-09-13" version="1">MGNVQSLRNKLDELAVNVRFLNAFRNISIMASTETWLMTSDPDEHVCIDGFKLVRGDRIPENVDKMRGNGLCVYTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CKF3</accession>
    <name>A0A9Q1CKF3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_05690</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CKF3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8046863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="104" mass="11173" checksum="E643486327396841" modified="2023-09-13" version="1">MPHIERKNPIVFGGGQRSPGVTGGQSLKTLLTQYLEKRYGMRKNPIIFGGGQRSSGVTGGQTLKTSLTRYLKIRYGMRKNPVIFGGGQRSSGVTGGQTLTTSLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CKF7</accession>
    <name>A0A9Q1CKF7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Solute carrier family 35 member G1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_05871</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CKF7"/>
    <dbReference type="OrthoDB" id="306876at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22911">
        <property type="entry name" value="ACYL-MALONYL CONDENSING ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22911:SF6">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER G1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="74"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="103"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="129"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="163"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="195"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="229"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="257"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="283"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="6">
        <location>
            <begin position="14"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="6">
        <location>
            <begin position="164"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8047002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="312" mass="34183" checksum="E3F5FB63293195FF" modified="2023-09-13" version="1">MTLEGLKQILYNQRGLYLALLSGVLFATQGLGTNLLTAALISPWSICFYYNLCNVFAIPWVNWKASKTYSISDLALYFLAGAFDTGNFALALQAYSFTTIGNASAILLSKPLWCALLACMLLRETFSLLDAGLVILNLLGVLLISKPPLIFESFTGYHDSDEFWGAMFALWGAISGGFQFVTIRSLVDRGIFDYFLLFVVRCTFGFPIFAVIVCANLDNYMNFHQLSQWIFLAIICLSGLGASLATYAALNSRDAKTVALIATVQVVVAYTFQFLIIGSSIDLLTVVGAVLIILSPVVFIIKSVIVEQNDVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CKI0</accession>
    <name>A0A9Q1CKI0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Lipase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_05914</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CKI0"/>
    <dbReference type="OrthoDB" id="19653at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45570">
        <property type="entry name" value="CARBOXYLIC ESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040492681" evidence="2">
        <location>
            <begin position="19"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="Carboxylesterase type B" evidence="3">
        <location>
            <begin position="27"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="75"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8047032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="111" mass="12435" checksum="6BD16D3DDE80DA01" modified="2023-09-13" version="1">MGWLRFVFIVALCSNASAKSFDAGTSEPIFETEYGIMVGMTVNDSDVFLGVPFVKPPVGELRFKDLVKLEKFTEEQKTKTNRPNCMQSDREGSPTVRNSLPVKCEQNLKIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CLD6</accession>
    <name>A0A9Q1CLD6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_05269</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CLD6"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8046564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="100" mass="11338" checksum="6006163D10794ED4" modified="2023-09-13" version="1">MNRTLACLVSPEECEALRKYFGVTEEEMRRIEAGDEDEELSDKEKTVRKMECLLTVIEEKGHLNPYNVNKLTDAVSTLGDEICISDLKGPMEGYSLHSKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CMC4</accession>
    <name>A0A9Q1CMC4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_06591</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CMC4"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031349">
        <property type="term" value="P:positive regulation of defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902533">
        <property type="term" value="P:positive regulation of intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009893">
        <property type="term" value="P:positive regulation of metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000754">
        <property type="entry name" value="Interleukin-1 receptor-associated kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051824">
        <property type="entry name" value="LRR_Rcpt-Like_S/T_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48006">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN DDB_G0281931-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48006:SF102">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN DDB_G0281931-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="160"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8047560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="432" mass="48852" checksum="7D91F63FD011DAAB" modified="2023-09-13" version="1">MMSTQSTCTPDTLLRKLKLTTVKSLAHVLDVENELSCDWRRFASLVPKRPGSTEMKYDMRKIQYLEHKLKRSGSSPTESIIQDLGTVNMQVKDIVKVLEIMGHYEAIKILLPEYSVPTEQAQPDTNHQIAVPEFDDSKIHHFTFQEVIALTANFNAAPLLEGGRKIGEGGFGAVFLGYFKNETKCAVKKLFKEELNPEVNILQQLQQEVETLLHLKHKNLVKIYGYCLENLCLVLEYIPEGNLCERLACEGSKHPLSWKSRVSIATGSARGINFLHNNNYIHRDVKSANILLDTDLVPKIVDFGLVRRLPDLKTSQVTSLVIGTNAYLAPECLAGNFSYKADVYSFGVVLLEIITGQRVFDKNREGGDLVSFIEDLEDDDAIINEVDKKAYPYDEDQVKKMYDVSSRCIQKKKLRPEMDEVYKELAAMCNPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CMD2</accession>
    <name>A0A9Q1CMD2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SCAN box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_00522</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CMD2"/>
    <dbReference type="OrthoDB" id="10063366at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF13">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCAN box" evidence="2">
        <location>
            <begin position="242"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="336"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="601"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="700"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="336"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="351"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="375"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="601"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="644"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="669"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="716"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="726"/>
            <end position="738"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8048272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="802" mass="90905" checksum="FC6D1A8E9C6C33B2" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CNJ8</accession>
    <name>A0A9Q1CNJ8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_00839</name>
        <name evidence="2" type="ORF">HOLleu_01056</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CNJ8"/>
    <dbReference type="OrthoDB" id="5978043at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF15">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8048498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8048655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="69" mass="7846" checksum="85CED3F4BB8DE5DB" modified="2023-09-13" version="1">MFTNEIGVIKGFQHKPELKLDVQPVQQNLRRIPFAVRDKLTHELRKLEAQGIIEKVPGASDWVSPIVDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CNL3</accession>
    <name>A0A9Q1CNL3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_00421</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CNL3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8048205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="133" mass="15476" checksum="A3CC29F607312156" modified="2023-09-13" version="1">MLITESAKLAVHSIVTFRTDDCKNLPVVINKSLLKRLKNIQPTAARSVTRKREFYFIKNDLIVLHWLPTKKRIVFKILVLLFKSLHQKSPSYIAEMLSERQNSRRLCSTTSSVPSLIEKRSHHFTLPERSFPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CP01</accession>
    <name>A0A9Q1CP01_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_01941</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CP01"/>
    <dbReference type="OrthoDB" id="6152551at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46601:SF1">
        <property type="entry name" value="ADF-H DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46601">
        <property type="entry name" value="ULP_PROTEASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8049262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="549" mass="63626" checksum="3329282BB0148617" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CPK5</accession>
    <name>A0A9Q1CPK5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Reverse transcriptase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_01157</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09274">
        <property type="entry name" value="RNase_HI_RT_Ty3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000115">
        <property type="entry name" value="Polyprotein of retroviral origin, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000032">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 297-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon gypsy-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054465">
        <property type="entry name" value="Integrase_p58-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041373">
        <property type="entry name" value="RT_RNaseH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22938">
        <property type="entry name" value="Integrase_p58_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17917">
        <property type="entry name" value="RT_RNaseH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="9">
        <location>
            <begin position="890"/>
            <end position="1048"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase" evidence="8">
        <location>
            <begin position="1304"/>
            <end position="1481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="94"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="359"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="623"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="723"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="359"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="374"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="398"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="623"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="667"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="692"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="739"/>
            <end position="763"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8048726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1696" mass="194428" checksum="3CCFE7C707C74770" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CSQ5</accession>
    <name>A0A9Q1CSQ5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HOLleu_03561</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Nanhai2018</strain>
            <tissue evidence="17">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CSQ5"/>
    <dbReference type="OrthoDB" id="337575at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031519">
        <property type="term" value="C:PcG protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17086">
        <property type="entry name" value="RAWUL_RING1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000100">
        <property type="entry name" value="E3 ubiquitin-protein ligase RING2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032443">
        <property type="entry name" value="RAWUL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043540">
        <property type="entry name" value="RING1/RING2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46076:SF3">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RING1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46076">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RING1 / RING 2 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16207">
        <property type="entry name" value="RAWUL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0678">Repressor</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="16">
        <location>
            <begin position="50"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="146"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="190"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ8050376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="346" mass="38812" checksum="AB4A03C5D99AC137" modified="2023-09-13" version="1">MAAVQSGGNAQPSKTWELSLYELHRTPQEAITDDTEIAVSPRSLHSELMCPICLDMLKMTYTTKECLHRFCQECIITALRSGNKECPTCRKKLVSKRSLRPDPNFDALISKIYPSRDEYEAHQERVLEKLAKHHSTQALANSIEEGMKQQALSRQQRVRKHHPLPSDQPQGEEPMDDQPPPSLPTPEVIPETREGGPSRQDAKRQRAVPGESSSSETGVTPELGEPSEPPEAMEVPSEIELVFRPHPSQPDPSLLPVRYLKTPTHAWIDHLAKYLSMRTSLEAKGSETSPGSSSQRPLEGNTEFTISVANQTGQYVELPGNTTLEQVNENYWKLSKPLELYYAVKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CT30</accession>
    <name>A0A9Q1CT30_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_04296</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CT30"/>
    <dbReference type="OrthoDB" id="5334309at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017868">
        <property type="entry name" value="Filamin/ABP280_repeat-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00630">
        <property type="entry name" value="Filamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50194">
        <property type="entry name" value="FILAMIN_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="1">
        <location>
            <begin position="102"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8050913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="616" mass="69387" checksum="F16B426480A6E5C7" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CTE3</accession>
    <name>A0A9Q1CTE3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_04501</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8051071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CTE3"/>
    <dbReference type="OrthoDB" id="409273at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09272">
        <property type="entry name" value="RNase_HI_RT_Ty1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8051071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="347" mass="37968" checksum="BFD914F2E11D23C6" modified="2023-09-13" version="1">MVECGATVSKVDPAVFYWVDSSDQVYGILACHVDDFIWGGDQEFEDIISKIKSTFKVGKESDNSFKYCGIDLLCDDNVIYLSQDSYTDGLTVIDISATRSVDKSAKLTAEEGHVLRSKVGQLLWLAHQSRPDLLFDVTKIANNLNKGSVGDILDINKIICKAKNSKLRLKFQSVSANLNEGVLHVVLYTDAALGNMPDGGSQAGYLIMLAGDSGTFSPICWNSKKIRRVVRSTLAAETLAMAEGIDASIFICTLLGELVYGKPEANLFPIVCFTDCKSLHDALKSPKIVSEKRLHLEISGIKEQLQKGQVKRVEWISSDLQLADCLTKKGAANNELRKALHSGVLTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CTX4</accession>
    <name>A0A9Q1CTX4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NLR family CARD domain-containing protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_04195</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CTX4"/>
    <dbReference type="OrthoDB" id="427518at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312">
        <property type="entry name" value="NACHT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312:SF2">
        <property type="entry name" value="NUCLEOTIDE-BINDING OLIGOMERIZATION DOMAIN-CONTAINING PROTEIN 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="433"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="311"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8050840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1103" mass="125989" checksum="A87D3848DAF49174" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CTZ2</accession>
    <name>A0A9Q1CTZ2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_03916</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CTZ2"/>
    <dbReference type="OrthoDB" id="6419989at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005911">
        <property type="term" value="C:cell-cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050839">
        <property type="term" value="F:cell adhesion molecule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013162">
        <property type="entry name" value="CD80_C2-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051275">
        <property type="entry name" value="Cell_adhesion_signaling"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11640:SF31">
        <property type="entry name" value="IRREGULAR CHIASM C-ROUGHEST PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11640">
        <property type="entry name" value="NEPHRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08205">
        <property type="entry name" value="C2-set_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040445669" description="Ig-like domain-containing protein" evidence="6">
        <location>
            <begin position="20"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="116"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="221"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8050634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="442" mass="50021" checksum="E9A1AD57DF8B8229" modified="2023-09-13" version="1">MAFLFFVLYLCSSVKQSCCQTTASLGPPISKIFAKSSGKIRLDCAVEESSLSKWLINDTRVTFGTDFIANIPKTKMRLLSNYSLIIAQVNTSDEGLYRCMHKDELVSMYQLEVEVPPVKTMLIVDGKVDPTELWMRMNDFVNITCCARGAKPAVYIQWVTNDIIHTPQTVVLLNEKDGTTFDSVSSLRFTMTQNAYISCKFIQGTSYRQEKKVVIWKAVTPSMSMVWQDMVITEPFFAVAGEEYELSCLLHSANSVNITWRINNTIMRSHNFVQDVGEMHNATKSILYLKPECCNEINVSCIASQRQSLEDLQSTIVVYTIEKPTIYILDAKGNIVTQLLAVYGSEYNLTCTAVGGKPHNITWFIMDVPSWRAVTYNTTNQSRVNVAFSKYTYMPDGQRFHQDNFFHISCSTFDDMALREIRYTINVTNIGGFESVTSKLTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1GYF9</accession>
    <name>A0A9Q1GYF9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_28575</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1GYF9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8029232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="129" mass="15163" checksum="70717A12FB2CAB8D" modified="2023-09-13" version="1">MPGIFIDEASAITTKYRQLYRLLAQERDARKPAQINLRHITAMLIDGVMRNYYTWAILDIARFSSMKAEDTFSRSIHVLLRIDETNLLLYRLVTGHRLLCNFHIVYSPLDCRIEGGRWTLMWYFLAVPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1GYP2</accession>
    <name>A0A9Q1GYP2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">adenylate cyclase</fullName>
            <ecNumber evidence="4">4.6.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HOLleu_29828</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Nanhai2018</strain>
            <tissue evidence="18">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP = 3',5'-cyclic AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15389"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <dbReference type="EC" id="4.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.1" evidence="4"/>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8027778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1GYP2"/>
    <dbReference type="OrthoDB" id="10035433at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
        <property type="term" value="F:adenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006171">
        <property type="term" value="P:cAMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1230:FF:000014">
        <property type="entry name" value="adenylate cyclase type 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018297">
        <property type="entry name" value="A/G_cyclase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45627">
        <property type="entry name" value="ADENYLATE CYCLASE TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45627:SF8">
        <property type="entry name" value="ADENYLATE CYCLASE TYPE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00452">
        <property type="entry name" value="GUANYLATE_CYCLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0115">cAMP biosynthesis</keyword>
    <keyword evidence="13 14" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="92"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="124"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="151"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="214"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="245"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="17">
        <location>
            <begin position="337"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="536"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="7"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="27"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="536"/>
            <end position="546"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ8027778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="630" mass="70885" checksum="D6AEA0D159A6030E" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H049</accession>
    <name>A0A9Q1H049_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Membrane-bound transcription factor site-2 protease</fullName>
            <ecNumber evidence="3">3.4.24.85</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Endopeptidase S2P</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_32634</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Zinc metalloprotease that mediates intramembrane proteolysis of proteins such as ATF6, ATF6B, SREBF1/SREBP1 and SREBF2/SREBP2. Catalyzes the second step in the proteolytic activation of the sterol regulatory element-binding proteins (SREBPs) SREBF1/SREBP1 and SREBF2/SREBP2: cleaves SREBPs within the first transmembrane segment, thereby releasing the N-terminal segment with a portion of the transmembrane segment attached. Mature N-terminal SREBP fragments shuttle to the nucleus and activate gene transcription. Also mediates the second step in the proteolytic activation of the cyclic AMP-dependent transcription factor ATF-6 (ATF6 and ATF6B). Involved in intramembrane proteolysis during bone formation. In astrocytes and osteoblasts, upon DNA damage and ER stress, mediates the second step of the regulated intramembrane proteolytic activation of the transcription factor CREB3L1, leading to the inhibition of cell-cycle progression.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleaves several transcription factors that are type-2 transmembrane proteins within membrane-spanning domains. Known substrates include sterol regulatory element-binding protein (SREBP) -1, SREBP-2 and forms of the transcriptional activator ATF6. SREBP-2 is cleaved at the site 477-DRSRILL-|-CVLTFLCLSFNPLTSLLQWGGA-505. The residues Asn-Pro, 11 residues distal to the site of cleavage in the membrane-spanning domain, are important for cleavage by S2P endopeptidase. Replacement of either of these residues does not prevent cleavage, but there is no cleavage if both of these residues are replaced.</text>
            <dbReference type="EC" id="3.4.24.85"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.85" evidence="3"/>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H049"/>
    <dbReference type="OrthoDB" id="69989at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031293">
        <property type="term" value="P:membrane protein intracellular domain proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905897">
        <property type="term" value="P:regulation of response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001193">
        <property type="entry name" value="MBTPS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008915">
        <property type="entry name" value="Peptidase_M50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13325:SF3">
        <property type="entry name" value="MEMBRANE-BOUND TRANSCRIPTION FACTOR SITE-2 PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13325">
        <property type="entry name" value="PROTEASE M50 MEMBRANE-BOUND TRANSCRIPTION FACTOR SITE 2 PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02163">
        <property type="entry name" value="Peptidase_M50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01000">
        <property type="entry name" value="SREBPS2PTASE"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="80"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="122"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="154"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="186"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="466"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M50" evidence="11">
        <location>
            <begin position="127"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00045828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8027481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="489" mass="54941" checksum="0983E2F03CB5535C" modified="2023-09-13" version="1">MLKTTWIALVLGFWSSVYLIDVFLKTKSHFNESYQNLQQRWGFQILTAQIRWYTTRLNRWIFRMGQCFPKFTFYWFEMGMWFGIICIFASVALLVMTLLKAFTEEKPEQVLTPVMPGVNVPVQHLSYFFAVLLVSGVLHELGHAIAAVREQVRINGFGIFVMVVYPGAFVDLHTESLMAISPKRQLKIYCAGIWHNFVLVLLGIILLVSLPTTMSLLYTTGEGVVITDLAKNSPVAGPRGLSIGSRLKSINGCPLLTADDWGRCLSATVFYPQNGYCQQIGKMQDVDNTVQLYKGLGGQVECCRNDSASDLCFYYQIGSSQENIQKKYQCLTARKTTEAARCHKNSDCLQVRKTVCVHPSLDNTSRLIRVLHDKGPPVLYVGDPYLFGFTVSVSGYIPKYNILPFYLPQTIDTFCKYLISLSGALAILNAVPCYALDGQWIFQAFIESYLTSLIPNPLDRQAVNSLGLLFGTLILMANVALALFTLLFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H0J9</accession>
    <name>A0A9Q1H0J9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_31177</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H0J9"/>
    <dbReference type="OrthoDB" id="10027367at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46670">
        <property type="entry name" value="ENDO/EXONUCLEASE/PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46670:SF3">
        <property type="entry name" value="ENDONUCLEASE_EXONUCLEASE_PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="1">
        <location>
            <begin position="121"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8028828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="432" mass="49822" checksum="3158E3ECBA384A63" modified="2023-09-13" version="1">MYYSRIQLLQLRYKHARSCAWFSDCIVQLRQSNILNNGITKTHRGVRAGVRVKARQHNGNLRIVHYEQFSVQSVLGQGQYQYSVAKQSTVSDRKANIFNLKPIAKELQHSRSVKIKFGLINTQSVRNKSAALLDYVFDQNIDVCCLTETWLSTNDDLVRFSLKPPGYDFQDSPRKNGRTGGGIGLLHRENIQASMVAQGEVDSFEFSEWLLQFQRKPLRLCVVYRPPYSAKHKVTKAVFHRQFADYLESVVTKPNPLLVVGDVNIHLNRLIDNDTRQFNDVLSSMALEQVVRDPTHVSGNILDVVIKRKDDDIVLSTTVHDELFSDHRVVSASICFPLPPLTVVDRLSRKIKQIDQESFQSDLRQVVNQAEGIDDLRALAKLYNTEFRAILDKHAPVVKKRVVIRPKLPWYNEDLQICKRRKLVRTIVWIWY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H1H5</accession>
    <name>A0A9Q1H1H5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Zinc finger protein 330</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_30858</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NOA36 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H1H5"/>
    <dbReference type="OrthoDB" id="10258894at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010531">
        <property type="entry name" value="NOA36"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13214">
        <property type="entry name" value="ZINC FINGER PROTEIN 330"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13214:SF1">
        <property type="entry name" value="ZINC FINGER PROTEIN 330"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06524">
        <property type="entry name" value="NOA36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="274"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="14"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="277"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8028576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="326" mass="36285" checksum="F69573DCEB131ADD" modified="2023-09-13" version="1">MPKKKTGARKKAEKQRQRQKEIRTAADSKSLVDWPCNYVMECDKCKRKQKNRAVCYFCMSVQKTIVCGHCGKMKCMMKSGDCVVKHPGQYTTGMGMVGAICDFCEAFICHGRKCLATHACECPLTDADCLECERDVWSHGGRIFKCSFCNGFLCEDDQFEHQASCQVLDGESYKCGSCNKLGQYSCLKCKSCFCDDHVRRKGFKYARGEPVPCPKCSYQLQDTKDLSMSVKKFAFGRQQNQDADGDEGGGGGYASYWENQGSGYSGASYSSYGGAVYDEDYEDEDESSSDDEEEEDGNTDENGEDEDDEISSPLSGLKLDPVYANS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H1M4</accession>
    <name>A0A9Q1H1M4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">C-Jun-amino-terminal kinase-interacting protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_31043</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the JIP scaffold family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10256043at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008432">
        <property type="term" value="F:JUN kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019894">
        <property type="term" value="F:kinesin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005078">
        <property type="term" value="F:MAP-kinase scaffold activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030159">
        <property type="term" value="F:signaling receptor complex adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1000:FF:000001">
        <property type="entry name" value="C-Jun-amino-terminal kinase-interacting protein 3 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1000">
        <property type="entry name" value="arf6 gtpase in complex with a specific effector, jip4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039911">
        <property type="entry name" value="JIP3/JIP4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032486">
        <property type="entry name" value="JIP_LZII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034743">
        <property type="entry name" value="RH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034744">
        <property type="entry name" value="RH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13886:SF4">
        <property type="entry name" value="JNK-INTERACTING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13886">
        <property type="entry name" value="JNK/SAPK-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16471">
        <property type="entry name" value="JIP_LZII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09744">
        <property type="entry name" value="RH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19056">
        <property type="entry name" value="WD40_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51776">
        <property type="entry name" value="RH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51777">
        <property type="entry name" value="RH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RH1" evidence="7">
        <location>
            <begin position="9"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="RH2" evidence="8">
        <location>
            <begin position="483"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="215"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="365"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="626"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="893"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1269"/>
            <end position="1300"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="63"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="417"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="369"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="634"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="894"/>
            <end position="912"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8028726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1314" mass="145596" checksum="631AE43860100EBE" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H3K9</accession>
    <name>A0A9Q1H3K9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_24906</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Complex I is composed of 45 different subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complex I NDUFB2 subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H3K9"/>
    <dbReference type="OrthoDB" id="6241903at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045271">
        <property type="term" value="C:respiratory chain complex I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032981">
        <property type="term" value="P:mitochondrial respiratory chain complex I assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026627">
        <property type="entry name" value="NDUFB2_animal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15223:SF1">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 BETA SUBCOMPLEX SUBUNIT 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15223">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE AGGG SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14813">
        <property type="entry name" value="NADH_B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0249">Electron transport</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="76"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="85"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8031648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="95" mass="11435" checksum="5F8961506F415C48" modified="2023-09-13" version="1">MFQTMKLLNSLRFTPRILHRTHQLPPRRHGHLVYRAFAPAPRKNQIIAQVILGSMWFWILWRFYHEPEDVFGHFPFPDPSKWTDEELGIPPDDED</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H4G6</accession>
    <name>A0A9Q1H4G6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="24">Diacylglycerol kinase</fullName>
            <shortName evidence="24">DAG kinase</shortName>
            <ecNumber evidence="24">2.7.1.107</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="ORF">HOLleu_26099</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="27">Nanhai2018</strain>
            <tissue evidence="27">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>1,2-di-(9Z-octadecenoyl)-sn-glycerol + ATP = 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40327"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:52333"/>
            <dbReference type="ChEBI" id="CHEBI:74546"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:40328"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycerol + ATP = 1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40323"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:75728"/>
            <dbReference type="ChEBI" id="CHEBI:77091"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:40324"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>a 1,2-diacyl-sn-glycerol + ATP = a 1,2-diacyl-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10272"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.107"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:10273"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="22">Glycerolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Lipid metabolism; glycerolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 24">Belongs to the eukaryotic diacylglycerol kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="27">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.107" evidence="24"/>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H4G6"/>
    <dbReference type="OrthoDB" id="242257at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004143">
        <property type="term" value="F:ATP-dependent diacylglycerol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20802">
        <property type="entry name" value="C1_DGK_typeIV_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000204">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.40:FF:000002">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10330:FF:000002">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037607">
        <property type="entry name" value="DGK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056383">
        <property type="entry name" value="DGKI-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000756">
        <property type="entry name" value="Diacylglycerol_kin_accessory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001206">
        <property type="entry name" value="Diacylglycerol_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11255">
        <property type="entry name" value="DIACYLGLYCEROL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11255:SF80">
        <property type="entry name" value="EYE-SPECIFIC DIACYLGLYCEROL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00609">
        <property type="entry name" value="DAGK_acc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00781">
        <property type="entry name" value="DAGK_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23578">
        <property type="entry name" value="DGKI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00045">
        <property type="entry name" value="DAGKa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00046">
        <property type="entry name" value="DAGKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50146">
        <property type="entry name" value="DAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 23" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="13 24" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12 24 27" id="KW-0418">Kinase</keyword>
    <keyword evidence="15" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 24" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 24" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DAGKc" evidence="26">
        <location>
            <begin position="267"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="23">
        <location>
            <begin position="859"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="23">
        <location>
            <begin position="895"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="229"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="25">
        <location>
            <begin position="233"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00060536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="RuleBase" id="RU361128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="EMBL" id="KAJ8032548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="964" mass="108784" checksum="DC5C8D126188210F" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H6E3</accession>
    <name>A0A9Q1H6E3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_20839</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H6E3"/>
    <dbReference type="OrthoDB" id="10066957at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1820">
        <property type="entry name" value="L1 transposable element, RRM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004244">
        <property type="entry name" value="Transposase_22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11505">
        <property type="entry name" value="L1 TRANSPOSABLE ELEMENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8034125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="177" mass="20638" checksum="FBE6BDBF70F5FF8B" modified="2023-09-13" version="1">MKKDLKPSKLRTYVLKISVIPEKEIEEHKKLGESHSQDLNKLERFSRRNNIRLIGIPEADQENCDTIVSEVLRKIELEDCKLERTHRDGTRIPGKPRHIIAKLTFSKDKVFLMKNARRSLANETYFVVDYLTLKDLKEKRRWKNEVSQLFCTFLGADGEPGMVAYLLFIHLNGVINS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H6I2</accession>
    <name>A0A9Q1H6I2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_21491</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H6I2"/>
    <dbReference type="OrthoDB" id="8869347at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="28"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8034588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="129" mass="14316" checksum="DD4BAC601E917ACC" modified="2023-09-13" version="1">MAFMVSTYLTTLSLRICVIVITGNLYKRTLTEAIGYETVVVRLGSNVTLPCENIQDSTNEGNLWIFNNISLLYAGYLRYAPADLANVNLDANGSLLIQNVERKNEASYVCRSGSKVILNQYVVIIEGML</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H765</accession>
    <name>A0A9Q1H765_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_19156</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8038157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H765"/>
    <dbReference type="OrthoDB" id="419189at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33332">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8038157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="358" mass="40614" checksum="F7437606D8796ED0" modified="2023-09-13" version="1">MGTQMLYEPAQSAYREHHGTETVLTRITDDIHCALDDGKGAYLVLLDLSAAFDTIIHHGMLLHRLEENLGLRYGTSVVTDVFRFENPTPNMSNFVRLRMESCSKNLRSWMKTSYLKLNDEKTELIVITSPYYRNMVQNAAIQVGSHVSEANTSVRNLGIIFDSSLNMEKHVSAVCKSSYHHLHNIKSIRKSLDNKTAETLIHAFVTSRLDCVNALLVGLPKRLLSKLQIVQNCAARVVMKVSRSEHITTTLHHLHWLPIQQRVTYKILLLTWGILHGQAPGYLTDIITPYIPARPLGSKYANRLVGPRFHTSRYGGRAFSRMGPRICNSMPEDQRGYNSLPVLKPKLKSYLFNNAFEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H7B2</accession>
    <name>A0A9Q1H7B2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_22144</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H7B2"/>
    <dbReference type="OrthoDB" id="7430131at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34239">
        <property type="entry name" value="APPLE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34239:SF2">
        <property type="entry name" value="TRANSPOSABLE ELEMENT P TRANSPOSASE_THAP9 CONSERVED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="349"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8035056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="368" mass="41373" checksum="9ED88D9E02DDB7F7" modified="2023-09-13" version="1">MDTEELDYQDSEEVLPLLDLPKEKSRVPAPSREGTSTRNVEFTDMLAQMQAQIQLLTEKVCKPSLDEPTSKPAASNQDALGDNVFPGNLESDSQLDFDQEIQNLLGASDEEPETNTISKITLGEQSSDEHVFDELVSDLTAASKTGPKVQEKVASLITSVCSTKLPPSKLKEKSEKYQIPENIELMQTTQVNKLIWDHLQLGTRTRDIKLQKIQMLNMKAMTALTSLLNDTLVSKAKLDKQSLLLKVTDALALMGSANVDLNQLRRDLIKPELKPEYKGLFDKSTPPSKLLFGDDIPQKLRDISDANKLTKRCMSPLRGRGQSYGRMKFRGRAHAGQRYQPFLGNRHAHWRRPYNRMPPKSQNKSQAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H840</accession>
    <name>A0A9Q1H840_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_16871</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H840"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8039222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="310" mass="35368" checksum="6DC2809280BB42F7" modified="2023-09-13" version="1">MHNLSFYTWARGVRTCSALPMTILAENALTFKKRASDPLTPSDQNSTLYEQYSIHQPRIQPQNCQFVALISVEGYVKSCTCVMSRMVIDLYQIEFFFYLFPLPGCVVGIEADVLTSCERRNPVTVIGDPGKAALCIFSGATRIICRALEGASRGVARAFLMGVEVVPYLPTEKKKCRLKVLISDAVRTENLCDFRHCLHWWRGDLADGSYGRMADQLCLKESWKMEDDSLNRRLIDCLMEESYSLVYIEEDVASSFGCVYGCEVRWRLSMEIHIATNSLRREETKKLGKSFPFHGTFNFFLTVMFRNEIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H8G6</accession>
    <name>A0A9Q1H8G6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_13704</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8039642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10056126at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01644">
        <property type="entry name" value="RT_pepA17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005312">
        <property type="entry name" value="DUF1759"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040676">
        <property type="entry name" value="DUF5641"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008042">
        <property type="entry name" value="Retrotrans_Pao"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331:SF5">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03564">
        <property type="entry name" value="DUF1759"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18701">
        <property type="entry name" value="DUF5641"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05380">
        <property type="entry name" value="Peptidase_A17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="2">
        <location>
            <begin position="1461"/>
            <end position="1647"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1239"/>
            <end position="1258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1248"/>
            <end position="1258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8039642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1764" mass="201980" checksum="335E0E20A3A0CE48" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H9D4</accession>
    <name>A0A9Q1H9D4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SPRY domain-containing SOCS box protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_18747</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SPSB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H9D4"/>
    <dbReference type="OrthoDB" id="5951542at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12876">
        <property type="entry name" value="SPRY_SOCS3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050672">
        <property type="entry name" value="FBXO45-Fsn/SPSB_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001496">
        <property type="entry name" value="SOCS_box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035754">
        <property type="entry name" value="SPRY_SPSB3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12245">
        <property type="entry name" value="SPRY DOMAIN CONTAINING SOCS BOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12245:SF5">
        <property type="entry name" value="SPRY DOMAIN-CONTAINING SOCS BOX PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50225">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="B30.2/SPRY" evidence="7">
        <location>
            <begin position="96"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="SOCS box" evidence="8">
        <location>
            <begin position="302"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="19"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="353"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="19"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="355"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8037829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="381" mass="42730" checksum="5B34B9F13B610A65" modified="2023-09-13" version="1">MIGPSCSQERTNCWGTMKRKAEEENEGCKRLRPYDEDDDDDAQQPERTETLQPSHHCDSSSNHNSDASDSEKEELLSRQPCLCTTGTTALHGRLAHFQPIISELPLLPTSLETTDDLLGQKSSVDFDWVWDDQGKVKSCVQLSRNKAEVRFHSNYSLGTASARSSEPLTEGQNYWEIKMVSPVYGTDMMVGIGTKRVDLDKFINQFCSMLGTDSEKESCGLSYLGYFYHGGRKTKFCDRFGQGAVIGVHLDLWRGSLSYYKNGNYLGVACSGLKGREWYPMVSSTAAKSSMCLIRTASFESSLQYLCCQKLRESIPARFDVLSVVDMPPGLKKFLKNNLYWLLGAHINTEDVQRKSTGTQTSSSKTIQRRKQSSPFSVKSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HAZ9</accession>
    <name>A0A9Q1HAZ9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Xenotropic and polytropic retrovirus receptor 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_16485</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SYG1 (TC 2.A.94) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HAZ9"/>
    <dbReference type="OrthoDB" id="9970435at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000822">
        <property type="term" value="F:inositol hexakisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016036">
        <property type="term" value="P:cellular response to phosphate starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14477">
        <property type="entry name" value="SPX_XPR1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004342">
        <property type="entry name" value="EXS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004331">
        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10783:SF103">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 53 MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10783">
        <property type="entry name" value="XENOTROPIC AND POLYTROPIC RETROVIRUS RECEPTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03124">
        <property type="entry name" value="EXS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03105">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51380">
        <property type="entry name" value="EXS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51382">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="240"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="315"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="360"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="386"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="417"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="447"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="487"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="524"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="555"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="domain" description="SPX" evidence="9">
        <location>
            <begin position="1"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="EXS" evidence="8">
        <location>
            <begin position="483"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="746"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="763"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="788"/>
            <end position="799"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8038923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="799" mass="94014" checksum="52B7B62D4E7684E9" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HEV2</accession>
    <name>A0A9Q1HEV2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_10970</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HEV2"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="53"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8043739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="90" mass="10376" checksum="6EA173C8280CD273" modified="2023-09-13" version="1">MDEIGESLFPSKADLRTIIKDHISEALSLPDFQEKLIESIRETLTDTISQNIHDAFQLELKAKQDQIDNLSGQLMALREQSEYLRNKTEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HGS3</accession>
    <name>A0A9Q1HGS3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">LIX1-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_02015</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LIX1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HGS3"/>
    <dbReference type="OrthoDB" id="6250996at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097352">
        <property type="term" value="P:autophagosome maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051436">
        <property type="entry name" value="Autophagy-related_EPG5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029270">
        <property type="entry name" value="LIX1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31139">
        <property type="entry name" value="ECTOPIC P GRANULES PROTEIN 5 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31139:SF6">
        <property type="entry name" value="PROTEIN LIMB EXPRESSION 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14954">
        <property type="entry name" value="LIX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8049317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="290" mass="32206" checksum="8183BC9CA6529388" modified="2023-09-13" version="1">MSQKPKGPPVDMTGRGGILQSPGLPMSDQKAKAVLKEAVDAVVNSFAKHTQGYGRVNVVEALQEFWQMKAARGADLKNGSLVVYESVPSSSPPYVCFVTLPGGSCFATFQNCTTKAEARRSAAKIALMNSVFNEHPSRKITHDFIRSAINDAKKSFQGSPEDEKDPNTSLGAFRFMLEANVNKSMLEFQELMTVFQLLHWNGSLKAMRERDCSRQEVVAHYSHRALDDDMRSQMALDWISREQEAAGIISRELHLADEEIEGARLAGRELRFPKEKKDILMLACSQLGPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HH47</accession>
    <name>A0A9Q1HH47_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_02566</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HH47"/>
    <dbReference type="OrthoDB" id="5974783at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8049707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="218" mass="25613" checksum="6D91BE9D80127F0B" modified="2023-09-13" version="1">MNDTFVIMGGDWNCVLDPSLDCDGYLHDNNPMARNAIVSLLNDHQLSDPYRVIHTNKKAYTWRKLNPFKQVRLDYFLTSNELLSVLDSVDIGLGYRSDHPLVNIKFVFNKQSPERSHWKFNTSLLRDKEYVNLVKNTIRETVLNYVATPINGENIENIPKQYIQFRIPESLLLEVFLMNIRGKSISYSSWKRKSYSIVKSKNLLMKLRQWKKILQRSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HJA3</accession>
    <name>A0A9Q1HJA3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Myb/SANT-like DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_01522</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HJA3"/>
    <dbReference type="OrthoDB" id="6115215at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028002">
        <property type="entry name" value="Myb_DNA-bind_5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23098">
        <property type="entry name" value="AGAP001331-PA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23098:SF16">
        <property type="entry name" value="REGULATORY PROTEIN ZESTE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13873">
        <property type="entry name" value="Myb_DNA-bind_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb/SANT-like DNA-binding" evidence="1">
        <location>
            <begin position="26"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8048989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="223" mass="25366" checksum="D3C761C63E1A9FA0" modified="2023-09-13" version="1">MVFCHHLLTLQFNFSQAARESTKVRREPNFSEEERLCLLNFVQQHKTKLLGKAGRPGHGDDKVEERKAKYWRLCAETIKAAGGHQRDWRKVRKQWFDLKDKAKRLKSEMGVTGGGPKPSSSGSLQLAMEVVAREALDGIRARTTEVDPLGHASVEMSQVLCKHHFIIERKVPLAQMKKLNHPSRKSLNLDSHMAIVFSFMKESSHTTSIVLVSRHFLSSFNHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HJG1</accession>
    <name>A0A9Q1HJG1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">N-acetylphosphatidylethanolamine-hydrolyzing phospholipase D</fullName>
            <ecNumber evidence="2">3.1.4.54</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_06267</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine + H2O = N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-ethanolamine + 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45528"/>
            <dbReference type="ChEBI" id="CHEBI:2700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:74546"/>
            <dbReference type="ChEBI" id="CHEBI:85277"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="4">
            <dbReference type="Rhea" id="RHEA:45529"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="5">Binds 2 zinc divalent cations per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAPE-PLD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.54" evidence="2"/>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HJG1"/>
    <dbReference type="OrthoDB" id="332863at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070290">
        <property type="term" value="F:N-acylphosphatidylethanolamine-specific phospholipase D activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070291">
        <property type="term" value="P:N-acylethanolamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070292">
        <property type="term" value="P:N-acylphosphatidylethanolamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009395">
        <property type="term" value="P:phospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024884">
        <property type="entry name" value="NAPE-PLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15032">
        <property type="entry name" value="N-ACYL-PHOSPHATIDYLETHANOLAMINE-HYDROLYZING PHOSPHOLIPASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15032:SF4">
        <property type="entry name" value="N-ACYL-PHOSPHATIDYLETHANOLAMINE-HYDROLYZING PHOSPHOLIPASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12706">
        <property type="entry name" value="Lactamase_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038896">
        <property type="entry name" value="NAPE-PLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0595">Phospholipid degradation</keyword>
    <keyword evidence="3" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="7">
        <location>
            <begin position="123"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="8"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="23"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="263"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="263"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="322"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR038896-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8047296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="370" mass="41761" checksum="5AC5F6106798559E" modified="2023-09-13" version="1">MAEAPPATENTINITTPVSENSSTKEEAPPTKSLEDVTHSIFKNGRYYNPWDTWEDPSFTKLLKWVATDRNKRNIPSQEELDKTLPIKTVIYKDLKSLPPPGRVRVMWIGHASTLVQFDGVTLLTDPVFGDIVGPKGIIGRKRYRPCPVSVDDLPNIDAVFTSHNHFDHLDENTVRKLNERFGESLRWFAPLGLKNWFCATGCQNVVEMDWWDESCLENRPEILVACTPTQHWSRRSVGDFCKTLWGSWVIKGPSASYFFAGDTGYCTGFKEIGKRYGPFSLAAIPIGAYEPVWFKKPQHISPEEAAQVHVDVRACKSIGIHWGTFPLGSEFYLDPPKEVRKGMEKLGQDPEDFLTLHHGEVKLLGTPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0Y8J6</accession>
    <name>A0A9Q0Y8J6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Carbamoyl-phosphate synthase [ammonia], mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_44847</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010001325">
        <property type="protein sequence ID" value="KAJ8017617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0Y8J6"/>
    <dbReference type="OrthoDB" id="434at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1380">
        <property type="entry name" value="Methylglyoxal synthase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011607">
        <property type="entry name" value="MGS-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036914">
        <property type="entry name" value="MGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02142">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00851">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52335">
        <property type="entry name" value="Methylglyoxal synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51855">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MGS-like" evidence="1">
        <location>
            <begin position="1"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8017617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="290" mass="33070" checksum="4C38BCB36624686B" modified="2023-09-13" version="1">MNVWHVPRPLKDWSPKGLRMFCSPLLQLFATEKTAKYLQDHNIPASTVVWPLAVIWKKWRENIHLPPGERGECVVLEKGILIQERTIGLVINLPNHNTKFVKDNYLIRRFAVDSGVPLLTNFELFATDKTAKYLQDRNIPASAVAWPLAVMEGEGREYPPATRYYKTVVGELLTNLLKNSFQITTDACRRNGALACNFLHLVSSPFSNLPDTNKGFEMYEHTCVTLNHKTVSFLSYFMSLKAGWKITFQIISKQLDFVKGVKNLLFVLMTCTSVKKEGNDLIQSDAIFFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YBF7</accession>
    <name>A0A9Q0YBF7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Fatty acid synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_43565</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000362">
        <property type="protein sequence ID" value="KAJ8018446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YBF7"/>
    <dbReference type="OrthoDB" id="3509362at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004312">
        <property type="term" value="F:fatty acid synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05195">
        <property type="entry name" value="enoyl_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049391">
        <property type="entry name" value="FAS_pseudo-KR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050091">
        <property type="entry name" value="PKS_NRPS_Biosynth_Enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43775">
        <property type="entry name" value="FATTY ACID SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43775:SF7">
        <property type="entry name" value="FATTY ACID SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21149">
        <property type="entry name" value="FAS_pseudo-KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="3" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="1" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="9">
        <location>
            <begin position="85"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8018446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="257" mass="28481" checksum="0EA607FA25DCFDC8" modified="2023-09-13" version="1">MYFVHHRCLFNTSQDSDSFTPLTELPQMQQLLKTDLVMNIFKNGCWGSYRHRFLNEGNHYLSIPTFPEELFTTPKNAYVDVLCPGDFSSLQWIQSPDDLKSGERCTVHYSSLNFRDVMLASGKVPRRSLPGKIITFAVENVVHKECLIGVEFAGHNSEGKRVMGIVEGQGIASHIYTEPCWLFDVPESWTLEDAATVPTAYCSVFYALVVRGQMKAGESILIHSGSGGVGQAAIAVALSYGCKVYTTVGGYIVTFTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YE46</accession>
    <name>A0A9Q0YE46_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_40907</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the IFIT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8021127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YE46"/>
    <dbReference type="OrthoDB" id="10043504at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10271">
        <property type="entry name" value="INTERFERON-INDUCED PROTEIN WITH TETRATRICOPEPTIDE REPEATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10271:SF0">
        <property type="entry name" value="INTERFERON-INDUCED PROTEIN WITH TETRATRICOPEPTIDE REPEATS 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040188358" evidence="4">
        <location>
            <begin position="16"/>
            <end position="885"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8021127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="885" mass="102927" checksum="A8C29E217476B00B" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YE85</accession>
    <name>A0A9Q0YE85_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Solute carrier family 25 member 36</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_38332</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YE85"/>
    <dbReference type="OrthoDB" id="269120at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015218">
        <property type="term" value="F:pyrimidine nucleotide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990519">
        <property type="term" value="P:pyrimidine nucleotide import into mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049562">
        <property type="entry name" value="SLC25A33/36-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45829">
        <property type="entry name" value="MITOCHONDRIAL CARRIER PROTEIN RIM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45829:SF4">
        <property type="entry name" value="MITOCHONDRIAL CARRIER PROTEIN RIM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="7"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="120"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="221"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8021202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="309" mass="34148" checksum="FF2328C3CA66ABE7" modified="2023-09-13" version="1">MFSQSISPQVIHLVAGGCGGTVGAVITCPLEVVKTRLQSSRASIKFLPTHTISPDGALTQVSRRSLSVIQSLRTICAEEGGTALFRGLGPNIVGIAPSRAIYFAAYANTKNFLNTKLEPESPSVHLLSAAVGGFTSSTCTNPLWLIKTRLQLEHKRGDRYANTWRAVKTVYRTEGIQGFYRGVTASYMGISETMIHFVIYEHIKKWLREQKAYNSDILTAKDFLTFMGAAATSKSIAATMAYPHEVARTRLREEGTKYRSFFQTLSTVLKEEGFRGWYGGLVAHLTRQIPNTAIILVVYELVVYSLNKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YEN5</accession>
    <name>A0A9Q0YEN5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_38511</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YEN5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8021343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="66" mass="7250" checksum="F425697A06601898" modified="2023-09-13" version="1">MGFHNHMSPINETAEHFTTVAADAGLGHAHIQIHLSNFEDEPECLLAKTCIVRFGVCWISEGTVSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YET2</accession>
    <name>A0A9Q0YET2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_40854</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8021085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YET2"/>
    <dbReference type="OrthoDB" id="6113439at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="160"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="222"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="237"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8021085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="269" mass="30460" checksum="1DF7D2B19324D7A7" modified="2023-09-13" version="1">MVELMLGQVANYCPIIARNSISKGATSLSSIWQMIRLHYGFQTSGSHFLDLSEIKLEVDERYGDLYQRLCAFVEDSLLTPESNITHHGEMVAEEEEMTPTIENFIVLTWLKLINPELPKLVKQRYGPELRSRTLASLKPEISQALDSLVSEIDSNITAMRASATSDHPRSKGRPARSKTPVRPLCKEANRPFSHFLSQYKYLPEEDRQYLLKARQISDILDVDADGDDDSDRPRSSLVESSTEPISANRIQTGRSPYIDMFFSESAQFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YFW9</accession>
    <name>A0A9Q0YFW9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_44402</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000851">
        <property type="protein sequence ID" value="KAJ8017911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YFW9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="107"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8017911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="176" mass="21068" checksum="FB48B75029D43FA0" modified="2023-09-13" version="1">MESSREFNFNAAFLELSRNKFVTSPSVEELDSLKKSEIVKVAKHYGIEFQPLMRKDEIKRYVLEYLVDESILPSTVLETAITVPTNNTFELKRLEMEMNKEIRLKEMEREREREEREREEREREREERERREEKAREHEFRLKQLELGVTKASDPKIGLDTGGFDVSKHVKFVPKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YFY9</accession>
    <name>A0A9Q0YFY9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_38395</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YFY9"/>
    <dbReference type="OrthoDB" id="64915at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006740">
        <property type="term" value="P:NADPH regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004104">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42840:SF3">
        <property type="entry name" value="BINDING ROSSMANN FOLD OXIDOREDUCTASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G10240)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42840">
        <property type="entry name" value="NAD(P)-BINDING ROSSMANN-FOLD SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02894">
        <property type="entry name" value="GFO_IDH_MocA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gfo/Idh/MocA-like oxidoreductase N-terminal" evidence="2">
        <location>
            <begin position="11"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="Gfo/Idh/MocA-like oxidoreductase C-terminal" evidence="3">
        <location>
            <begin position="143"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8021249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="345" mass="38724" checksum="8A6056EF0F260777" modified="2023-09-13" version="1">MSSESGERVGLALFGIGRAGEVHLKNILRERKADLKWIVEENLSHAQEIIEKNLVDVPVIKPEDSDKVFSDPSVRGIIICTPTNSHADLVKKGLKSGKAVFCEKPIANAVEITETCYAEAEKYGQPLFCAMNRRFDPSVRLMKQRMTRGDIGQVYSIKACYRDCPCPPIQFLKTSGGIFHDCGVHFIDIICWILGEGPISVYVQAHAFHNDIAGLGDVDQALIVMKFPSGCTGSIDLSRLSVYGYDQRLEIFGSKGMLEQRNKVPTSVVHYSDIGATHDPIEPTFRERYRESFELELVHFLNVQKGIEKEPSVKKEDALRMCRIADACKESYRTGQQIFLNNYQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YGF4</accession>
    <name>A0A9Q0YGF4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Eukaryotic translation initiation factor 4 gamma 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_38756</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YGF4"/>
    <dbReference type="OrthoDB" id="514777at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003307">
        <property type="entry name" value="W2_domain"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51363">
        <property type="entry name" value="W2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="W2" evidence="2">
        <location>
            <begin position="120"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8021519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="273" mass="31545" checksum="7907688E7403EC1A" modified="2023-09-13" version="1">MNKKPNQQSLDHKYFLINFYTTFSHFYKSSVHLRLNDYLAVTEQIAEFTSQYWSSLGEIIGAMVSGGTKSLEFLKEVIQTTPGDEKAADNLYQVKKLWDTSKFSWSLFIADKWSIKSFVGKQDLDFTLSPEERNARKPPKQVPQRNENAVGEDVRKTKEFIHALVLAVCLSSMVEGDDGPYTKCQRELLKRRSVLIQRYIRRNKDLEMIALQVVEALYASVGNPRGVLRTMCEVLYDEDVISCETFAVWRNSEDFSEENELATKKFFESLDQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YGR4</accession>
    <name>A0A9Q0YGR4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_43623</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000386">
        <property type="protein sequence ID" value="KAJ8018406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YGR4"/>
    <dbReference type="OrthoDB" id="6367123at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8018406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="145" mass="15740" checksum="9945E7842F96E749" modified="2023-09-13" version="1">MVILIEGNTALPLHRVVPKAHIRYFGSPRNLLLIVQSGGARSQVISQQTLWQMPAVGARQSRVSQPGEREHVSGSGPTSYSARASADVGPAHEPSQSGPEPMVEEPRSPSPDEVAHQVEQDFLAAKQCDQNRLFRPAESTLAKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YHD5</accession>
    <name>A0A9Q0YHD5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_38738</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YHD5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8021506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="96" mass="10930" checksum="AD6521CBCF74DF23" modified="2023-09-13" version="1">MLYSRKRSLTGSPKIQKNETQQLVTHFIHNNSVGYAVIDPHYLTRYAVIDPQYLTGYAVIDPQYLIGYAVNDLQYLIGYAVTDPQYLSGYAVIDPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YKZ2</accession>
    <name>A0A9Q0YKZ2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_35556</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YKZ2"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8023220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="96" mass="11181" checksum="0103A887FB6A88C1" modified="2023-09-13" version="1">MCEQTIVFAVRNPMKLYCVRSGTFHAYERYLSVCAHITIAIICAVHASHERLGTYPAYVRTYLHTTERTKLSTLTAIRCNSELWPRWTLKEILLTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YM38</accession>
    <name>A0A9Q0YM38_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">E3 ubiquitin-protein ligase</fullName>
            <ecNumber evidence="11">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HOLleu_35119</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Nanhai2018</strain>
            <tissue evidence="15">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Ubiquitin ligase protein which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8 11">Belongs to the E3 ubiquitin-protein ligase UBR1-like family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="11"/>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8025030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="15304at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071596">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process via the N-end rule pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd19673">
        <property type="entry name" value="UBR-box_UBR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.30:FF:000002">
        <property type="entry name" value="Putative e3 ubiquitin-protein ligase ubr3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044046">
        <property type="entry name" value="E3_ligase_UBR-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039164">
        <property type="entry name" value="UBR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055194">
        <property type="entry name" value="UBR1-like_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003126">
        <property type="entry name" value="Znf_UBR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21497:SF39">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE UBR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21497">
        <property type="entry name" value="UBIQUITIN LIGASE E3 ALPHA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18995">
        <property type="entry name" value="PRT6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22960">
        <property type="entry name" value="WHD_UBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02207">
        <property type="entry name" value="zf-UBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00396">
        <property type="entry name" value="ZnF_UBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51157">
        <property type="entry name" value="ZF_UBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 11" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 9 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBR-type" evidence="14">
        <location>
            <begin position="77"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="13">
        <location>
            <begin position="1256"/>
            <end position="1317"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="UBR-type" evidence="10">
        <location>
            <begin position="77"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="993"/>
            <end position="1035"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1110"/>
            <end position="1139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="999"/>
            <end position="1020"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU366018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ8025030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1853" mass="210655" checksum="E2E57397DBE2B963" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YMD8</accession>
    <name>A0A9Q0YMD8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Electron transfer flavoprotein regulatory factor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_36751</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I LYR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8024116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YMD8"/>
    <dbReference type="OrthoDB" id="10258445at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090324">
        <property type="term" value="P:negative regulation of oxidative phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022904">
        <property type="term" value="P:respiratory electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20265">
        <property type="entry name" value="Complex1_LYR_ETFRF1_LYRM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008011">
        <property type="entry name" value="Complex1_LYR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045296">
        <property type="entry name" value="Complex1_LYR_ETFRF1_LYRM5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052000">
        <property type="entry name" value="ETFRF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21024:SF0">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN REGULATORY FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21024">
        <property type="entry name" value="GROWTH HORMONE-INDUCIBLE SOLUBLE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05347">
        <property type="entry name" value="Complex1_LYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Complex 1 LYR protein" evidence="2">
        <location>
            <begin position="16"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8024116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="91" mass="11128" checksum="C2C3DBAAE5B43431" modified="2023-09-13" version="1">MTQLQQLASTNLRYAVKTLYKNQLIFLGREYPKGEKYFRDRCHHAFMKNKDETDPEKIKELIGRGEYVLKELEALYMLRKYRALKKRYYDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YML4</accession>
    <name>A0A9Q0YML4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Coiled-coil domain-containing protein 91</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_36886</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8024221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YML4"/>
    <dbReference type="OrthoDB" id="6146069at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090160">
        <property type="term" value="P:Golgi to lysosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034592">
        <property type="entry name" value="CCDC91"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35072">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 91"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35072:SF1">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 91"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="70"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="162"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="210"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="300"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="22"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="105"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="185"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8024221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="528" mass="59204" checksum="7CC41F5E0D0026C0" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YN16</accession>
    <name>A0A9Q0YN16_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Short-chain collagen C4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_38293</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8023187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YN16"/>
    <dbReference type="OrthoDB" id="5983381at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005581">
        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051077">
        <property type="entry name" value="Ca-dependent_lectin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24024">
        <property type="entry name" value="PULMONARY SURFACTANT-ASSOCIATED PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24024:SF18">
        <property type="entry name" value="SHORT-CHAIN COLLAGEN C4-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0176">Collagen</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040310004" evidence="3">
        <location>
            <begin position="19"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="114"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="461"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="660"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="417"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="148"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="467"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="513"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="573"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="667"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="686"/>
            <end position="706"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8023187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="925" mass="100377" checksum="A87B858E85E314DF" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YQQ5</accession>
    <name>A0A9Q0YQQ5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_34526</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YQQ5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8024582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="52" mass="5826" checksum="546FEEEA7BBEEAEC" modified="2023-09-13" version="1">MLHTERKNPIVFGGGQRSPGVTGGQTLKTLLTQYLEVGSLDEFHTYYVDALW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YRH1</accession>
    <name>A0A9Q0YRH1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_32258</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YRH1"/>
    <dbReference type="OrthoDB" id="6138802at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47018">
        <property type="entry name" value="CXC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8027189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="77" mass="8469" checksum="4E245D31348320BE" modified="2023-09-13" version="1">MISWAAYHASQQGVRPPEDLNVAHTSLLPLFHDKAHSVAMIRHLMDIVKTAVGVLNPGQIPALTCDQPLYTLAKQIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YTJ0</accession>
    <name>A0A9Q0YTJ0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Vacuolar protein sorting-associated protein 13B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_33320</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YTJ0"/>
    <dbReference type="OrthoDB" id="445152at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026854">
        <property type="entry name" value="VPS13_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039782">
        <property type="entry name" value="VPS13B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12517:SF0">
        <property type="entry name" value="INTERMEMBRANE LIPID TRANSFER PROTEIN VPS13B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12517">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 13B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12624">
        <property type="entry name" value="VPS13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Chorein N-terminal" evidence="2">
        <location>
            <begin position="8"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8025696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="341" mass="38929" checksum="FF1E325EE1B55342" modified="2023-09-13" version="1">MSHFLIEYLSSYLKRYVQKYIKSIMPKNLEVWLRKGDLVMKKLDLRLDVLEKELPHLPISFVSGHIHKLEIHIPWTKLFSKIAVEITINTIEFVVKLKHGASSDGKSQSIVSSQIKKYCSGTGSKCTPGYIQSFKRRVVNNAHIVIKNLILKYVEEDTVLSVNVKSVESYATDADWKRACVDLSPSELVLRRLCTFSDITVCLDKCNASGKIEVYQEPVICHWNLTTCIHMTFKNLAANAASVIKLSTFCDELNLSLSETRLPMYIRLVQLVLALYYGSLDLPETRTGNLEKESEETSHKEDAAVGNVYIERLIKGNHQQIQNNVYNLWLLTQILKPHQIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BAR5</accession>
    <name>A0A9Q1BAR5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Cytochrome P450 2J3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HOLleu_41476</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Nanhai2018</strain>
            <tissue evidence="16">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8019760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BAR5"/>
    <dbReference type="OrthoDB" id="2789670at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000238">
        <property type="entry name" value="Cytochrome P450 2A6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050182">
        <property type="entry name" value="Cytochrome_P450_fam2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300">
        <property type="entry name" value="CYTOCHROME P450 508A4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300:SF417">
        <property type="entry name" value="CYTOCHROME P450 508B1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 13 14" id="KW-0349">Heme</keyword>
    <keyword evidence="10 13 14" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0492">Microsome</keyword>
    <keyword evidence="11 14" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="9 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040511199" evidence="15">
        <location>
            <begin position="24"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="13">
        <location>
            <position position="434"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ8019760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="496" mass="55649" checksum="A2E2640D011B45E8" modified="2023-09-13" version="1">MELTILLALTFFLLMAWLFAKRGQNFPPGPNGLPIVGSIFALLNNPFQKLKEYADTYGPVYTLKIGPIWVIMLNDAATIQELFTTRSGEFSERPKLIADVTGFGENGIVLLPSGSYHKRQKKVAMSLFRDIGVGNEKFDHLLRPYLDSVMKSLPDNPCIVNIENVCSETVSNIIGGFTFGDKVYNDNSDVSQEIRKLIRQNFENNPILIVGNTYLPKKLCSLLGHFVDMENNISKMKKLLKRMMESHYFKKENKHQLNCFVPKYLEEYATSHAGEELWGNSKEENQLVGIIIDFFQAGCETTAVSLQWLLLLLAANPEVQTKIQQEIDDLFGEELPSLQQKSKLNYTVATMYESLRIRPSAPLGAPHCTKEDTTILGYQIPAGTPILSNLLSAQLNPQTFPDPHVFKPERFLDENGNICNLGSLLTFGTGPRTCLGEQLAKSILLSIVINILQKYTLRKKSEGCVDLSFQHGINLLATENELLFSPRTSQMDSEQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BCE7</accession>
    <name>A0A9Q1BCE7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">PHD finger protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_39914</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1884872at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15552">
        <property type="entry name" value="PHD_PHF3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21541">
        <property type="entry name" value="SPOC_PHF3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.5.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.30">
        <property type="entry name" value="Transcription elongation factor S-II, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006576">
        <property type="entry name" value="BRK_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037259">
        <property type="entry name" value="BRK_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012921">
        <property type="entry name" value="SPOC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003618">
        <property type="entry name" value="TFIIS_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036575">
        <property type="entry name" value="TFIIS_cen_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11477:SF51">
        <property type="entry name" value="PROTEIN PARTNER OF SNF, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11477">
        <property type="entry name" value="TRANSCRIPTION FACTOR S-II ZINC FINGER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07533">
        <property type="entry name" value="BRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07744">
        <property type="entry name" value="SPOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07500">
        <property type="entry name" value="TFIIS_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00592">
        <property type="entry name" value="BRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00510">
        <property type="entry name" value="TFS2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160481">
        <property type="entry name" value="BRK domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46942">
        <property type="entry name" value="Elongation factor TFIIS domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51321">
        <property type="entry name" value="TFIIS_CENTRAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="11">
        <location>
            <begin position="401"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="TFIIS central" evidence="12">
        <location>
            <begin position="789"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="78"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="123"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="467"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="722"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="928"/>
            <end position="960"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="990"/>
            <end position="1094"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1271"/>
            <end position="1326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1346"/>
            <end position="1372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1384"/>
            <end position="1426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1541"/>
            <end position="1637"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="1472"/>
            <end position="1518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="82"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="228"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="280"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="299"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="377"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="470"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="517"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="549"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="722"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="949"/>
            <end position="960"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="1000"/>
            <end position="1010"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1011"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1034"/>
            <end position="1094"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1271"/>
            <end position="1281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1305"/>
            <end position="1316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1587"/>
            <end position="1600"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8020343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1637" mass="182522" checksum="7A10040DDDD2F606" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BDA9</accession>
    <name>A0A9Q1BDA9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_38345</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the histone deacetylase HD2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BDA9"/>
    <dbReference type="OrthoDB" id="8117402at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.340">
        <property type="entry name" value="Nucleoplasmin core domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041232">
        <property type="entry name" value="NPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394:SF48">
        <property type="entry name" value="ZINC FINGER PROTEIN 771"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17800">
        <property type="entry name" value="NPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="343"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="371"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="399"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="427"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="108"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="158"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="182"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8021213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="454" mass="50925" checksum="B0511630D01F9D9C" modified="2023-09-13" version="1">MIWGLALQPGHEYTEIFEEETHLSMATLECRPGKFSEEQPSSYIVVRTLQGEYLLCFLEKGTTPQQSLDLVLQPQEEVTFCVEGTATIFITGYTASVYTGGDGGHSQAVELSETSDKESFSNKEYNEIVVKTEEETTPEGEGADGSNVGGVIIVTVNSENEVEDDKPDVSFASSHFDSQSVSTDVISECSEEEEPNGEGFNEGANKPASVKSPRDDIALRSEVLQGGRSMVKSSDSETLTCHICSKTFLSLETRQQHALNAHRETIVSHDTKSFGELTSEAQTDLSTTDSNLVCQPGTSQNVRRDVDPDRNCRVQYSWNSSKRSGLRYTDGAPKVILNKKKIHKCQYCHKMFDGIGNRKAHERIHTGERPFKCRFCPQGFGRKSHCIIHERRHTGEKPFRCKFCEWAFADSSNYNAHVKTHTGETPYHCRICHKGFSRRFSCQRHENSHMGEKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BEV5</accession>
    <name>A0A9Q1BEV5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Nuclear pore complex protein Nup153</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">153 kDa nucleoporin</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Nucleoporin Nup153</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">HOLleu_35325</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="23">Nanhai2018</strain>
            <tissue evidence="23">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the NUP153 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8025191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BEV5"/>
    <dbReference type="OrthoDB" id="79830at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1060.10:FF:000001">
        <property type="entry name" value="Nuclear pore complex protein Nup153"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1060.10">
        <property type="entry name" value="Zinc finger, RanBP2-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036443">
        <property type="entry name" value="Znf_RanBP2_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00547">
        <property type="entry name" value="ZnF_RBZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90209">
        <property type="entry name" value="Ran binding protein zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01358">
        <property type="entry name" value="ZF_RANBP2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50199">
        <property type="entry name" value="ZF_RANBP2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="13" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0811">Translocation</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 20" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RanBP2-type" evidence="22">
        <location>
            <begin position="5"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="29"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="100"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="310"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="35"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="101"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="127"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="144"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="158"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="186"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="209"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="310"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00060842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00068609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00078197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00079437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KAJ8025191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="364" mass="37747" checksum="2E8E0E1B734C87DB" modified="2023-09-13" version="1">MFKPASASWVCDSCMINNPADKDQCVACSTPKPGSSQPSQESSCDSQGTLGRMGMSGGAFSLGNFKFSGFSSNSSAFSAGSGCGSSGFNKATPGTFSCGQTLSQSSNTTPSSSAGFSFPPGGFSFTQPKPETSQPSDTGGFKMSAASSFSFTAPTPPGSESTKSAVDSATKEQPLSKPTEPIPGQPTVTTPTFNFGSGMPLFTYGGTGQQKPSSGFSFDSTPGSSALTSQTVGKPSFSTTTQAGSVFGGTQATSIFGATPEKGSLSAFKGFEFGTKQSEPKTEQKEAISFSDFVKNQGEFSFCLNVDNVASSPSKSGAKSPVKSPGLSKSKEYYQEEERDKIYFEPVVTLPENYKSETGKKMKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BEW6</accession>
    <name>A0A9Q1BEW6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_37954</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BEW6"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8022923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="74" mass="8056" checksum="2C40E33762EE8293" modified="2023-09-13" version="1">MTTDTSQVHTSQNGRETTNKQQDEGVVTTQPRSTSKDEDGNQDEEEEVGRGQECGLICICIEITECCCDLLCGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BG67</accession>
    <name>A0A9Q1BG67_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_35523</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8025340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BG67"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="122"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8025340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="143" mass="16220" checksum="0E6934C239AB7138" modified="2023-09-13" version="1">MHYGKRKNLIVFGGGQRSFGVTRGQTLKTLFTQYLKMQYGKRKNPIVFGGGQRSFGVTRGQTLKTLFTRYLKMHYGERKNPIAFGGGQRSFKVTSGQTLKTLFKRYLKMHYGRRKNGIIFGGGQRSSGVTRRHSLKTSLNTTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BHH9</accession>
    <name>A0A9Q1BHH9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Metabotropic glutamate receptor 8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_31770</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BHH9"/>
    <dbReference type="OrthoDB" id="5984008at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13953">
        <property type="entry name" value="7tm_classC_mGluR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.30">
        <property type="entry name" value="GPCR, family 3, nine cysteines domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000337">
        <property type="entry name" value="GPCR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038550">
        <property type="entry name" value="GPCR_3_9-Cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050726">
        <property type="entry name" value="mGluR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24060">
        <property type="entry name" value="METABOTROPIC GLUTAMATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01176">
        <property type="entry name" value="GABABRECEPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00248">
        <property type="entry name" value="GPCRMGR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 14" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040251389" evidence="12">
        <location>
            <begin position="29"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="566"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="602"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="635"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="677"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="730"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="759"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="791"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="13">
        <location>
            <begin position="565"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="828"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="837"/>
            <end position="847"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ8026825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="873" mass="99173" checksum="3C8E8BB3F1F5D958" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BHM5</accession>
    <name>A0A9Q1BHM5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Adhesion G-protein coupled receptor D1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_34161</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8026343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BHM5"/>
    <dbReference type="OrthoDB" id="1100386at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000058">
        <property type="entry name" value="Adhesion G protein-coupled receptor F5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.220.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046338">
        <property type="entry name" value="GAIN_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017983">
        <property type="entry name" value="GPCR_2_secretin-like_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12011">
        <property type="entry name" value="ADHESION G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12011:SF471">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 2 PROFILE 2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00249">
        <property type="entry name" value="GPCRSECRETIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00650">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040402900" evidence="9">
        <location>
            <begin position="30"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="639"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="675"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="707"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="745"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="790"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="838"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="867"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="10">
        <location>
            <begin position="641"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8026343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="949" mass="105605" checksum="9555312481BB0C76" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BIA7</accession>
    <name>A0A9Q1BIA7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_31673</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BIA7"/>
    <dbReference type="OrthoDB" id="2544694at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8026749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="137" mass="15957" checksum="CA358E490C9919EF" modified="2023-09-13" version="1">MVCLIGVPVAMNQMAQVFLAARTEHWCSVEEWENDVDACADLTEDAYLGCIHKYRNASIPIKEDDDGKYYSQCQKFDATYPNWDDEYYAGKETNDTIDCDEGWVYDQSEYTRTIRTDVCISFYFLFIHIYSTCTQLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BKR5</accession>
    <name>A0A9Q1BKR5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">BTB/POZ domain-containing protein kctd15</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_30607</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BKR5"/>
    <dbReference type="OrthoDB" id="2414723at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14499:SF136">
        <property type="entry name" value="GH08630P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14499">
        <property type="entry name" value="POTASSIUM CHANNEL TETRAMERIZATION DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Potassium channel tetramerisation-type BTB" evidence="1">
        <location>
            <begin position="7"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8028389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="116" mass="13403" checksum="00B03EC079AFE2D6" modified="2023-09-13" version="1">MASSGCVVLNVGGTKYMTSEETMRGLCLSQDQLNLRDNEGNIFIDRDGVLFRYILNYLRERRLILPEDFPEIDALQTEAIFFCLHEMVKDIKRYKEEKECNFLFGCCLSFLASQNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BKU5</accession>
    <name>A0A9Q1BKU5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_30795</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BKU5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon gypsy-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF13">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCAN box" evidence="2">
        <location>
            <begin position="248"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase zinc-binding" evidence="3">
        <location>
            <begin position="803"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="342"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="609"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="709"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="342"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="357"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="384"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="609"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="633"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="653"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="678"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="725"/>
            <end position="749"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF17921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8028533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="921" mass="104838" checksum="ECF18F47A24432EC" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BLH0</accession>
    <name>A0A9Q1BLH0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Fatty acid synthase</fullName>
            <ecNumber evidence="5">1.1.1.100</ecNumber>
            <ecNumber evidence="2">1.3.1.39</ecNumber>
            <ecNumber evidence="6">2.3.1.41</ecNumber>
            <ecNumber evidence="4">2.3.1.85</ecNumber>
            <ecNumber evidence="3">3.1.2.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="68" type="ORF">HOLleu_31113</name>
    </gene>
    <organism evidence="68 69">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="68" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="68">Nanhai2018</strain>
            <tissue evidence="68">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="32">Fatty acid synthetase is a multifunctional enzyme that catalyzes the de novo biosynthesis of long-chain saturated fatty acids starting from acetyl-CoA and malonyl-CoA in the presence of NADPH. This multifunctional protein contains 7 catalytic activities and a site for the binding of the prosthetic group 4'-phosphopantetheine of the acyl carrier protein ([ACP]) domain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="39">
            <text>(2E)-butenoyl-[ACP] + NADPH + H(+) = butanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41812"/>
            <dbReference type="Rhea" id="RHEA-COMP:9627"/>
            <dbReference type="Rhea" id="RHEA-COMP:9628"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78453"/>
            <dbReference type="ChEBI" id="CHEBI:78454"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="39">
            <dbReference type="Rhea" id="RHEA:41813"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="62">
            <text>(2E)-decenoyl-[ACP] + NADPH + H(+) = decanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41864"/>
            <dbReference type="Rhea" id="RHEA-COMP:9639"/>
            <dbReference type="Rhea" id="RHEA-COMP:9640"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78467"/>
            <dbReference type="ChEBI" id="CHEBI:78468"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="62">
            <dbReference type="Rhea" id="RHEA:41865"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="46">
            <text>(2E)-dodecenoyl-[ACP] + NADPH + H(+) = dodecanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41880"/>
            <dbReference type="Rhea" id="RHEA-COMP:9643"/>
            <dbReference type="Rhea" id="RHEA-COMP:9644"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65264"/>
            <dbReference type="ChEBI" id="CHEBI:78472"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="46">
            <dbReference type="Rhea" id="RHEA:41881"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="41">
            <text>(2E)-hexadecenoyl-[ACP] + NADPH + H(+) = hexadecanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41912"/>
            <dbReference type="Rhea" id="RHEA-COMP:9651"/>
            <dbReference type="Rhea" id="RHEA-COMP:9652"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78481"/>
            <dbReference type="ChEBI" id="CHEBI:78483"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="41">
            <dbReference type="Rhea" id="RHEA:41913"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="42">
            <text>(2E)-hexenoyl-[ACP] + NADPH + H(+) = hexanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41832"/>
            <dbReference type="Rhea" id="RHEA-COMP:9631"/>
            <dbReference type="Rhea" id="RHEA-COMP:9632"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78458"/>
            <dbReference type="ChEBI" id="CHEBI:78459"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="42">
            <dbReference type="Rhea" id="RHEA:41833"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="55">
            <text>(2E)-octadecenoyl-[ACP] + NADPH + H(+) = octadecanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41928"/>
            <dbReference type="Rhea" id="RHEA-COMP:9655"/>
            <dbReference type="Rhea" id="RHEA-COMP:9656"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78489"/>
            <dbReference type="ChEBI" id="CHEBI:78495"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="55">
            <dbReference type="Rhea" id="RHEA:41929"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="48">
            <text>(2E)-octenoyl-[ACP] + NADPH + H(+) = octanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41848"/>
            <dbReference type="Rhea" id="RHEA-COMP:9635"/>
            <dbReference type="Rhea" id="RHEA-COMP:9636"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78462"/>
            <dbReference type="ChEBI" id="CHEBI:78463"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="48">
            <dbReference type="Rhea" id="RHEA:41849"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="57">
            <text>(2E)-tetradecenoyl-[ACP] + NADPH + H(+) = tetradecanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41896"/>
            <dbReference type="Rhea" id="RHEA-COMP:9647"/>
            <dbReference type="Rhea" id="RHEA-COMP:9648"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78475"/>
            <dbReference type="ChEBI" id="CHEBI:78477"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="57">
            <dbReference type="Rhea" id="RHEA:41897"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="31">
            <text>(3R)-hydroxybutanoyl-[ACP] = (2E)-butenoyl-[ACP] + H2O</text>
            <dbReference type="Rhea" id="RHEA:41808"/>
            <dbReference type="Rhea" id="RHEA-COMP:9626"/>
            <dbReference type="Rhea" id="RHEA-COMP:9627"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:78451"/>
            <dbReference type="ChEBI" id="CHEBI:78453"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="31">
            <dbReference type="Rhea" id="RHEA:41809"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>(3R)-hydroxydecanoyl-[ACP] = (2E)-decenoyl-[ACP] + H2O</text>
            <dbReference type="Rhea" id="RHEA:41860"/>
            <dbReference type="Rhea" id="RHEA-COMP:9638"/>
            <dbReference type="Rhea" id="RHEA-COMP:9639"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:78466"/>
            <dbReference type="ChEBI" id="CHEBI:78467"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:41861"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>(3R)-hydroxydodecanoyl-[ACP] = (2E)-dodecenoyl-[ACP] + H2O</text>
            <dbReference type="Rhea" id="RHEA:41876"/>
            <dbReference type="Rhea" id="RHEA-COMP:9642"/>
            <dbReference type="Rhea" id="RHEA-COMP:9643"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:78470"/>
            <dbReference type="ChEBI" id="CHEBI:78472"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:41877"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="30">
            <text>(3R)-hydroxyhexadecanoyl-[ACP] = (2E)-hexadecenoyl-[ACP] + H2O</text>
            <dbReference type="Rhea" id="RHEA:41908"/>
            <dbReference type="Rhea" id="RHEA-COMP:9650"/>
            <dbReference type="Rhea" id="RHEA-COMP:9651"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:78480"/>
            <dbReference type="ChEBI" id="CHEBI:78481"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="30">
            <dbReference type="Rhea" id="RHEA:41909"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>(3R)-hydroxyhexanoyl-[ACP] = (2E)-hexenoyl-[ACP] + H2O</text>
            <dbReference type="Rhea" id="RHEA:41828"/>
            <dbReference type="Rhea" id="RHEA-COMP:9630"/>
            <dbReference type="Rhea" id="RHEA-COMP:9631"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:78457"/>
            <dbReference type="ChEBI" id="CHEBI:78458"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:41829"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>(3R)-hydroxyoctadecanoyl-[ACP] = (2E)-octadecenoyl-[ACP] + H2O</text>
            <dbReference type="Rhea" id="RHEA:41924"/>
            <dbReference type="Rhea" id="RHEA-COMP:9654"/>
            <dbReference type="Rhea" id="RHEA-COMP:9655"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:78488"/>
            <dbReference type="ChEBI" id="CHEBI:78489"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="29">
            <dbReference type="Rhea" id="RHEA:41925"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>(3R)-hydroxyoctanoyl-[ACP] = (2E)-octenoyl-[ACP] + H2O</text>
            <dbReference type="Rhea" id="RHEA:41844"/>
            <dbReference type="Rhea" id="RHEA-COMP:9634"/>
            <dbReference type="Rhea" id="RHEA-COMP:9635"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:78461"/>
            <dbReference type="ChEBI" id="CHEBI:78462"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:41845"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>(3R)-hydroxytetradecanoyl-[ACP] = (2E)-tetradecenoyl-[ACP] + H2O</text>
            <dbReference type="Rhea" id="RHEA:41892"/>
            <dbReference type="Rhea" id="RHEA-COMP:9646"/>
            <dbReference type="Rhea" id="RHEA-COMP:9647"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:78474"/>
            <dbReference type="ChEBI" id="CHEBI:78475"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="28">
            <dbReference type="Rhea" id="RHEA:41893"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="43">
            <text>3-oxobutanoyl-[ACP] + NADPH + H(+) = (3R)-hydroxybutanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41804"/>
            <dbReference type="Rhea" id="RHEA-COMP:9625"/>
            <dbReference type="Rhea" id="RHEA-COMP:9626"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78450"/>
            <dbReference type="ChEBI" id="CHEBI:78451"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="43">
            <dbReference type="Rhea" id="RHEA:41805"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="37">
            <text>3-oxodecanoyl-[ACP] + NADPH + H(+) = (3R)-hydroxydecanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41856"/>
            <dbReference type="Rhea" id="RHEA-COMP:9637"/>
            <dbReference type="Rhea" id="RHEA-COMP:9638"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78464"/>
            <dbReference type="ChEBI" id="CHEBI:78466"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="37">
            <dbReference type="Rhea" id="RHEA:41857"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="58">
            <text>3-oxododecanoyl-[ACP] + NADPH + H(+) = (3R)-hydroxydodecanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41872"/>
            <dbReference type="Rhea" id="RHEA-COMP:9641"/>
            <dbReference type="Rhea" id="RHEA-COMP:9642"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78469"/>
            <dbReference type="ChEBI" id="CHEBI:78470"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="58">
            <dbReference type="Rhea" id="RHEA:41873"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="59">
            <text>3-oxohexadecanoyl-[ACP] + NADPH + H(+) = (3R)-hydroxyhexadecanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41904"/>
            <dbReference type="Rhea" id="RHEA-COMP:9649"/>
            <dbReference type="Rhea" id="RHEA-COMP:9650"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78478"/>
            <dbReference type="ChEBI" id="CHEBI:78480"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="59">
            <dbReference type="Rhea" id="RHEA:41905"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="50">
            <text>3-oxohexanoyl-[ACP] + NADPH + H(+) = (3R)-hydroxyhexanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41824"/>
            <dbReference type="Rhea" id="RHEA-COMP:9629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9630"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78456"/>
            <dbReference type="ChEBI" id="CHEBI:78457"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="50">
            <dbReference type="Rhea" id="RHEA:41825"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="34">
            <text>3-oxooctadecanoyl-[ACP] + NADPH + H(+) = (3R)-hydroxyoctadecanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41920"/>
            <dbReference type="Rhea" id="RHEA-COMP:9653"/>
            <dbReference type="Rhea" id="RHEA-COMP:9654"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78487"/>
            <dbReference type="ChEBI" id="CHEBI:78488"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="34">
            <dbReference type="Rhea" id="RHEA:41921"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="60">
            <text>3-oxooctanoyl-[ACP] + NADPH + H(+) = (3R)-hydroxyoctanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41840"/>
            <dbReference type="Rhea" id="RHEA-COMP:9633"/>
            <dbReference type="Rhea" id="RHEA-COMP:9634"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78460"/>
            <dbReference type="ChEBI" id="CHEBI:78461"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="60">
            <dbReference type="Rhea" id="RHEA:41841"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="54">
            <text>3-oxotetradecanoyl-[ACP] + NADPH + H(+) = (3R)-hydroxytetradecanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41888"/>
            <dbReference type="Rhea" id="RHEA-COMP:9645"/>
            <dbReference type="Rhea" id="RHEA-COMP:9646"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78473"/>
            <dbReference type="ChEBI" id="CHEBI:78474"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="54">
            <dbReference type="Rhea" id="RHEA:41889"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="36">
            <text>a (3R)-hydroxyacyl-[ACP] + NADP(+) = a 3-oxoacyl-[ACP] + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17397"/>
            <dbReference type="Rhea" id="RHEA-COMP:9916"/>
            <dbReference type="Rhea" id="RHEA-COMP:9945"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78776"/>
            <dbReference type="ChEBI" id="CHEBI:78827"/>
            <dbReference type="EC" id="1.1.1.100"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="36">
            <dbReference type="Rhea" id="RHEA:17399"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>a (3R)-hydroxyacyl-[ACP] = a (2E)-enoyl-[ACP] + H2O</text>
            <dbReference type="Rhea" id="RHEA:13097"/>
            <dbReference type="Rhea" id="RHEA-COMP:9925"/>
            <dbReference type="Rhea" id="RHEA-COMP:9945"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:78784"/>
            <dbReference type="ChEBI" id="CHEBI:78827"/>
            <dbReference type="EC" id="4.2.1.59"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:13098"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="51">
            <text>a 2,3-saturated acyl-[ACP] + NADP(+) = a (2E)-enoyl-[ACP] + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22564"/>
            <dbReference type="Rhea" id="RHEA-COMP:9925"/>
            <dbReference type="Rhea" id="RHEA-COMP:9926"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78784"/>
            <dbReference type="ChEBI" id="CHEBI:78785"/>
            <dbReference type="EC" id="1.3.1.39"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="51">
            <dbReference type="Rhea" id="RHEA:22566"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="49">
            <text>a fatty acyl-[ACP] + malonyl-[ACP] + H(+) = a 3-oxoacyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:22836"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:9916"/>
            <dbReference type="Rhea" id="RHEA-COMP:14125"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78776"/>
            <dbReference type="ChEBI" id="CHEBI:138651"/>
            <dbReference type="EC" id="2.3.1.41"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="49">
            <dbReference type="Rhea" id="RHEA:22837"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="33">
            <text>acetyl-CoA + n malonyl-CoA + 2n NADPH + 2n H(+) = a long-chain fatty acid + (n+1) CoA + n CO2 + 2n NADP(+).</text>
            <dbReference type="EC" id="2.3.1.85"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="44">
            <text>acetyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxobutanoyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:41800"/>
            <dbReference type="Rhea" id="RHEA-COMP:9621"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9625"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78446"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78450"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="44">
            <dbReference type="Rhea" id="RHEA:41801"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="61">
            <text>butanoyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxohexanoyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:41820"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9628"/>
            <dbReference type="Rhea" id="RHEA-COMP:9629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78454"/>
            <dbReference type="ChEBI" id="CHEBI:78456"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="61">
            <dbReference type="Rhea" id="RHEA:41821"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="56">
            <text>decanoyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxododecanoyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:41868"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9640"/>
            <dbReference type="Rhea" id="RHEA-COMP:9641"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78468"/>
            <dbReference type="ChEBI" id="CHEBI:78469"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="56">
            <dbReference type="Rhea" id="RHEA:41869"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="40">
            <text>dodecanoyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxotetradecanoyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:41884"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9644"/>
            <dbReference type="Rhea" id="RHEA-COMP:9645"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:65264"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78473"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="40">
            <dbReference type="Rhea" id="RHEA:41885"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="53">
            <text>hexadecanoyl-[ACP] + H2O = hexadecanoate + holo-[ACP] + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41932"/>
            <dbReference type="Rhea" id="RHEA-COMP:9652"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78483"/>
            <dbReference type="EC" id="3.1.2.14"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="53">
            <dbReference type="Rhea" id="RHEA:41933"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="45">
            <text>hexadecanoyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxooctadecanoyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:41916"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9652"/>
            <dbReference type="Rhea" id="RHEA-COMP:9653"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78483"/>
            <dbReference type="ChEBI" id="CHEBI:78487"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="45">
            <dbReference type="Rhea" id="RHEA:41917"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="35">
            <text>hexanoyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxooctanoyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:41836"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9632"/>
            <dbReference type="Rhea" id="RHEA-COMP:9633"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78459"/>
            <dbReference type="ChEBI" id="CHEBI:78460"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="35">
            <dbReference type="Rhea" id="RHEA:41837"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="52">
            <text>holo-[ACP] + acetyl-CoA = acetyl-[ACP] + CoA</text>
            <dbReference type="Rhea" id="RHEA:41788"/>
            <dbReference type="Rhea" id="RHEA-COMP:9621"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78446"/>
            <dbReference type="EC" id="2.3.1.38"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="52">
            <dbReference type="Rhea" id="RHEA:41789"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="63">
            <text>octanoyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxodecanoyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:41852"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9636"/>
            <dbReference type="Rhea" id="RHEA-COMP:9637"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78463"/>
            <dbReference type="ChEBI" id="CHEBI:78464"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="63">
            <dbReference type="Rhea" id="RHEA:41853"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="47">
            <text>tetradecanoyl-[ACP] + H2O = tetradecanoate + holo-[ACP] + H(+)</text>
            <dbReference type="Rhea" id="RHEA:30123"/>
            <dbReference type="Rhea" id="RHEA-COMP:9648"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30807"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78477"/>
            <dbReference type="EC" id="3.1.2.14"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="47">
            <dbReference type="Rhea" id="RHEA:30124"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="38">
            <text>tetradecanoyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxohexadecanoyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:41900"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9648"/>
            <dbReference type="Rhea" id="RHEA-COMP:9649"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78477"/>
            <dbReference type="ChEBI" id="CHEBI:78478"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="38">
            <dbReference type="Rhea" id="RHEA:41901"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism.</text>
    </comment>
    <comment type="caution">
        <text evidence="68">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.100" evidence="5"/>
    <dbReference type="EC" id="1.3.1.39" evidence="2"/>
    <dbReference type="EC" id="2.3.1.41" evidence="6"/>
    <dbReference type="EC" id="2.3.1.85" evidence="4"/>
    <dbReference type="EC" id="3.1.2.14" evidence="3"/>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="329835at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019171">
        <property type="term" value="F:(3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004316">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004313">
        <property type="term" value="F:[acyl-carrier-protein] S-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141148">
        <property type="term" value="F:enoyl-[acyl-carrier-protein] reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004312">
        <property type="term" value="F:fatty acid synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016297">
        <property type="term" value="F:fatty acyl-[ACP] hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031177">
        <property type="term" value="F:phosphopantetheine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05195">
        <property type="entry name" value="enoyl_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08954">
        <property type="entry name" value="KR_1_FAS_SDR_x"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00833">
        <property type="entry name" value="PKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1200.10:FF:000013">
        <property type="entry name" value="Fatty acid synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.180.10:FF:000015">
        <property type="entry name" value="Fatty acid synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000209">
        <property type="entry name" value="Polyketide synthase Pks12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="3.40.366.10">
        <property type="entry name" value="Malonyl-Coenzyme A Acyl Carrier Protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="3.10.129.110">
        <property type="entry name" value="Polyketide synthase dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001227">
        <property type="entry name" value="Ac_transferase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014043">
        <property type="entry name" value="Acyl_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049391">
        <property type="entry name" value="FAS_pseudo-KR"/>
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    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018201">
        <property type="entry name" value="Ketoacyl_synth_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014031">
        <property type="entry name" value="Ketoacyl_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014030">
        <property type="entry name" value="Ketoacyl_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057326">
        <property type="entry name" value="KR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016036">
        <property type="entry name" value="Malonyl_transacylase_ACP-bd"/>
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    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
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    <dbReference type="InterPro" id="IPR032821">
        <property type="entry name" value="PKS_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020841">
        <property type="entry name" value="PKS_Beta-ketoAc_synthase_dom"/>
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    <dbReference type="InterPro" id="IPR042104">
        <property type="entry name" value="PKS_dehydratase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020807">
        <property type="entry name" value="PKS_DH"/>
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    <dbReference type="InterPro" id="IPR049552">
        <property type="entry name" value="PKS_DH_N"/>
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    <dbReference type="InterPro" id="IPR013968">
        <property type="entry name" value="PKS_KR"/>
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    <dbReference type="InterPro" id="IPR049900">
        <property type="entry name" value="PKS_mFAS_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050091">
        <property type="entry name" value="PKS_NRPS_Biosynth_Enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020806">
        <property type="entry name" value="PKS_PP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
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    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
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    <dbReference type="InterPro" id="IPR001031">
        <property type="entry name" value="Thioesterase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43775">
        <property type="entry name" value="FATTY ACID SYNTHASE"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR43775:SF7">
        <property type="entry name" value="FATTY ACID SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00698">
        <property type="entry name" value="Acyl_transf_1"/>
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    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
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    <dbReference type="Pfam" id="PF21149">
        <property type="entry name" value="FAS_pseudo-KR"/>
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    <dbReference type="Pfam" id="PF16197">
        <property type="entry name" value="KAsynt_C_assoc"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00109">
        <property type="entry name" value="ketoacyl-synt"/>
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    <dbReference type="Pfam" id="PF02801">
        <property type="entry name" value="Ketoacyl-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08659">
        <property type="entry name" value="KR"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF21089">
        <property type="entry name" value="PKS_DH_N"/>
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    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
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    <dbReference type="Pfam" id="PF00975">
        <property type="entry name" value="Thioesterase"/>
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    <dbReference type="SMART" id="SM00827">
        <property type="entry name" value="PKS_AT"/>
        <property type="match status" value="1"/>
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    <dbReference type="SMART" id="SM00826">
        <property type="entry name" value="PKS_DH"/>
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    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00822">
        <property type="entry name" value="PKS_KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00825">
        <property type="entry name" value="PKS_KS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00823">
        <property type="entry name" value="PKS_PP"/>
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    </dbReference>
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        <property type="entry name" value="ACP-like"/>
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    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FabD/lysophospholipase-like"/>
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        <property type="entry name" value="GroES-like"/>
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        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
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    <dbReference type="SUPFAM" id="SSF55048">
        <property type="entry name" value="Probable ACP-binding domain of malonyl-CoA ACP transacylase"/>
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    </dbReference>
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        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
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        <property type="entry name" value="Thiolase-like"/>
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        <property type="entry name" value="CARRIER"/>
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        <property type="entry name" value="KS3_1"/>
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        <property type="entry name" value="KS3_2"/>
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    <dbReference type="PROSITE" id="PS52019">
        <property type="entry name" value="PKS_MFAS_DH"/>
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    <proteinExistence type="predicted"/>
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    <keyword evidence="21" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="14" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="20" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="22" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="19" id="KW-0520">NAD</keyword>
    <keyword evidence="15" id="KW-0521">NADP</keyword>
    <keyword evidence="18" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="10" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="69" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0702">S-nitrosylation</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
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        <location>
            <begin position="2"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="PKS/mFAS DH" evidence="67">
        <location>
            <begin position="861"/>
            <end position="1129"/>
        </location>
    </feature>
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        <location>
            <begin position="2164"/>
            <end position="2241"/>
        </location>
    </feature>
    <feature type="region of interest" description="N-terminal hotdog fold" evidence="64">
        <location>
            <begin position="861"/>
            <end position="981"/>
        </location>
    </feature>
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        <location>
            <begin position="993"/>
            <end position="1129"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for dehydratase activity" evidence="64">
        <location>
            <position position="892"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor; for dehydratase activity" evidence="64">
        <location>
            <position position="1042"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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    <sequence length="2546" mass="279085" checksum="CDBB5CFD04F46333" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BNN1</accession>
    <name>A0A9Q1BNN1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">LINE-1 reverse transcriptase-like</fullName>
        </submittedName>
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        <name evidence="1" type="ORF">HOLleu_29571</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8030007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BNN1"/>
    <dbReference type="OrthoDB" id="10065625at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061343">
        <property type="term" value="P:cell adhesion involved in heart morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007508">
        <property type="term" value="P:larval heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33395:SF22">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33395">
        <property type="entry name" value="TRANSCRIPTASE, PUTATIVE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8030007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="188" mass="20848" checksum="A0B46852882E3B22" modified="2023-09-13" version="1">MELPQYGIPSLKHEGKTITDAKLKADLLNNYFTSVFTLNNHKSIPDKGPPPLPPMPCIVVTAPGVLKLLSELKTRKAIGPDKIPSVLLKTVANEITPVIVSFFQQSLNTGIFPDKLKKANVTPIPKKGSKADVKNYPPISLTSILSKCLEHIMVSNFMSHLESNSILTPYQHGFRKHRSTVTQRFAYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BNS4</accession>
    <name>A0A9Q1BNS4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Proline-rich transmembrane protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_29427</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CD225/Dispanin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BNS4"/>
    <dbReference type="OrthoDB" id="6083617at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051423">
        <property type="entry name" value="CD225/Dispanin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007593">
        <property type="entry name" value="CD225/Dispanin_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14948:SF25">
        <property type="entry name" value="DUF4190 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14948">
        <property type="entry name" value="NG5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04505">
        <property type="entry name" value="CD225"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="53"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="106"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="23"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8029901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="157" mass="17201" checksum="2EF09EF8640F8E03" modified="2023-09-13" version="1">MADNRKPPQQSYPPQQMHPPPQQGDSTQPGTSSQQSPVTTMVIRDPQIPAKDYLVFAIFVTMCCCWPLGIVAIIKALNVRKLNGRGDYSLAQQASQKAKKWSIGSLICGIVLQLFFIVFVVVFVVLIVLAINAANNNSNTASSNSNYGLDRLFDGLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BRM7</accession>
    <name>A0A9Q1BRM7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_25144</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BRM7"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8031818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="63" mass="6664" checksum="D9AADEAC98079095" modified="2023-09-13" version="1">MGSRRDLRSRTSFLAHARLVTSLAARKFGHCNEKSRAAARACLGKQPALLTRTSAGLVGGQPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BSF2</accession>
    <name>A0A9Q1BSF2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_25313</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BSF2"/>
    <dbReference type="OrthoDB" id="10261408at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="3"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="29"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="37"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="77"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="103"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8031942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="153" mass="16812" checksum="203B465056C967FA" modified="2023-09-13" version="1">MSHRCPICSSTFTRSFNLRRHIERVHKRVEEDLEGQDGNSVASEGSGKQSGDYAHSSEGSYDDRSESDSNESASQPMEEDSVTDRDDTDSCNSSDTEGADRAEEFEEDDTVTDSGGEEEEGRGNVSGTMVTLAALTEMNKAMEKILKRALQNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BSL2</accession>
    <name>A0A9Q1BSL2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_25540</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BSL2"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8032117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="167" mass="22408" checksum="49A0F40447364AAA" modified="2023-09-13" version="1">MEMAKMKRDKKTERKLTKGRRKKCKNRKKERKKERKKERKKERKKERKKERKKERKKERKKERKKERKKERNKERKKERKKERRKKERRKKERKKKERRKKERKERKKERKKEERKKERKKERKKERKKERKKERKKERKKERKKERKKERKKERKKERKKERRKER</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BSY5</accession>
    <name>A0A9Q1BSY5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_25786</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BSY5"/>
    <dbReference type="OrthoDB" id="5985928at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00882">
        <property type="entry name" value="Ras_like_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="239"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="G" evidence="2">
        <location>
            <begin position="5"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8032297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="322" mass="36143" checksum="09E2D503485397FD" modified="2023-09-13" version="1">MDTIRIMSVGRSGVGKSTIGNMFLLGNGKGRFKTSMASTYCTERSECRASEKGDIEYVDVPGIPCTDPKNNGKFYDIMISEAKKELNVILFVFKRGKEDYEAYKLAEVLFRELNKCNTTKILVINDFTSYEFDQPPAEADFNDEMERIKEITKIDFTSMIAVNGKTMPTKLTEVKNLISEKKALKSEHLRNFTELGMYVDKLRADKCKEDKEIRREEQKLNENKSRSQFNEHVDQAGNVLGLFSGALGILSLLSVAGALPIAVGGALGTVALKAFSSRNKTEQKNEEKSAAARLDNLRQRKAEKAFKTVSKEFEDLKKVLNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BTT3</accession>
    <name>A0A9Q1BTT3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Protein PAT1-like 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_26062</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">P-body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PAT1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BTT3"/>
    <dbReference type="OrthoDB" id="74835at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033962">
        <property type="term" value="P:P-body assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039900">
        <property type="entry name" value="Pat1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019167">
        <property type="entry name" value="PAT1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21551:SF0">
        <property type="entry name" value="PROTEIN ASSOCIATED WITH TOPO II RELATED-1, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21551">
        <property type="entry name" value="TOPOISOMERASE II-ASSOCIATED PROTEIN PAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09770">
        <property type="entry name" value="PAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="mRNA decay factor PAT1" evidence="8">
        <location>
            <begin position="462"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="60"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="108"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="228"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="418"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="498"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="60"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="439"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="504"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="529"/>
            <end position="554"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF09770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8032514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="883" mass="97874" checksum="A368F8201C57AC80" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BU41</accession>
    <name>A0A9Q1BU41_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Retroviral polymerase SH3-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_26181</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BU41"/>
    <dbReference type="OrthoDB" id="413361at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039537">
        <property type="entry name" value="Retrotran_Ty1/copia-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057670">
        <property type="entry name" value="SH3_retrovirus"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648">
        <property type="entry name" value="TRANSPOSASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648:SF11">
        <property type="entry name" value="TRANSPOSON TY4-P GAG-POL POLYPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25597">
        <property type="entry name" value="SH3_retrovirus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0229">DNA integration</keyword>
    <keyword evidence="9" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="8" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="3" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Retroviral polymerase SH3-like" evidence="10">
        <location>
            <begin position="72"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF25597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8032614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="173" mass="20727" checksum="21E5ABA50B2EF55D" modified="2023-09-13" version="1">MEQWKGTGERCSKWGRCLLIESGLPKTLWPYAVQIAVHIRNRCYNNRVKQTPYYMRTVRKPNLSKMWIFGSDCYAYKHNQRKLDPRCEKGIFVGYDKNSPAYLIYYPVSGKVIKHRLVKFIRKDSVAQQTQTDETWFDKADMYLKQHTCVKRQIQGRQVFPRAKLRWIILKMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BVM1</accession>
    <name>A0A9Q1BVM1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_23686</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BVM1"/>
    <dbReference type="OrthoDB" id="5987792at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005312">
        <property type="entry name" value="DUF1759"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008042">
        <property type="entry name" value="Retrotrans_Pao"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331:SF4">
        <property type="entry name" value="PEPTIDASE S1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03564">
        <property type="entry name" value="DUF1759"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05380">
        <property type="entry name" value="Peptidase_A17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="404"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="341"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="97"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8033444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="725" mass="81720" checksum="011FF1288EE19B53" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BVN1</accession>
    <name>A0A9Q1BVN1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Fibrinogen-like protein A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_23911</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BVN1"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="CDD" id="cd00053">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00087">
        <property type="entry name" value="FReD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19941">
        <property type="entry name" value="TIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050373">
        <property type="entry name" value="Fibrinogen_C-term_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024731">
        <property type="entry name" value="NELL2-like_EGF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040941">
        <property type="entry name" value="GGGWT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143">
        <property type="entry name" value="FIBRINOGEN/TENASCIN/ANGIOPOEITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12947">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00186">
        <property type="entry name" value="FBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1 3" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EGF-like" evidence="4">
        <location>
            <begin position="75"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="5">
        <location>
            <begin position="110"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8033610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="353" mass="40163" checksum="164175A554E4AF2A" modified="2023-09-13" version="1">MEYGNCTCQATCEDPDGKNCYATCFEEQTCLCVEGFLKKGDECVPSQECSCFFGERVIPVFVNTECTRRCQCQGNALACEDNYGCSSDATCEERDGVRQCYCNDGYTGDGQNCEVATDCADIYNSNITDSGVYTIKPTNWNGSAFEVYCNMTDEGGWTVFQRRVNGSQDFYQGWNRYKYGFGSPNHEQWLGNEKIYYLTNQRSYQLRIDLVYGTGRPYFAKYDVFRINDKNDKYRLADLGSHFGTAAKDRLNSHENKLFSTHDEDNDGWSSYNCAERHRGGWWYAEYSVDTPCAISEYYCDWWPSGNNSCGICTQANLNGDYNSVTPGTSIDWESSSENDCDIIYTEMKIKPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BXI3</accession>
    <name>A0A9Q1BXI3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Neurotrypsin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_21335</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BXI3"/>
    <dbReference type="OrthoDB" id="536948at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000005">
        <property type="entry name" value="Neurotrypsin isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000007">
        <property type="entry name" value="Soluble scavenger receptor cysteine-rich domain-containing protein SSC5D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48071">
        <property type="entry name" value="SRCR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48071:SF28">
        <property type="entry name" value="SRCR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00530">
        <property type="entry name" value="SRCR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00258">
        <property type="entry name" value="SPERACTRCPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00420">
        <property type="entry name" value="SRCR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="SRCR" evidence="7">
        <location>
            <begin position="17"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="7">
        <location>
            <begin position="121"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="82"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="159"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="190"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8034478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="242" mass="27073" checksum="B39BF3EA40146F2F" modified="2023-09-13" version="1">MLLRSRRISGGNGIQGIRLIGKTGQPQRVDILHDEKWGTVCGDEEWDLDDARVVCRQLGYHNATNSYKNISGRIVWMSEVQCQGDETYISSCRHQKFQQADRCLIENDAGVRCEGNGLQGIRLVGGSSDSSGRVEVFMNRGWGTICDDGWDMEDAIVVCRQLGYQNATSAPIEEFFGRGSASVCMTDVQCTGNETSLTECTKEERQTYNWRNSYDAGVVCEGKKNTFRLRKLGKCDKETKTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BXJ5</accession>
    <name>A0A9Q1BXJ5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_21355</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="7550652at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01644">
        <property type="entry name" value="RT_pepA17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005312">
        <property type="entry name" value="DUF1759"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008042">
        <property type="entry name" value="Retrotrans_Pao"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331:SF1">
        <property type="entry name" value="GAG-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03564">
        <property type="entry name" value="DUF1759"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05380">
        <property type="entry name" value="Peptidase_A17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="1">
        <location>
            <begin position="1255"/>
            <end position="1390"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8034493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1390" mass="157145" checksum="DF667A651593EC6F" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BXK6</accession>
    <name>A0A9Q1BXK6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Monocarboxylate transporter 12</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_21527</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BXK6"/>
    <dbReference type="OrthoDB" id="6499973at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008028">
        <property type="term" value="F:monocarboxylic acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF284">
        <property type="entry name" value="EG:103B4.3 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="53"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="84"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="108"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="172"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="242"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="275"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="308"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="335"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="372"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="397"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="3">
        <location>
            <begin position="1"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8034615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="501" mass="54080" checksum="03BBE26851E104C9" modified="2023-09-13" version="1">MAAQIVPAAPDGGWGWIALAGSVFNEILLAGYVEGMGSFYVEWQKYFNVGAPEVGWVGILVALNGPFGCLVAGGLSTRFGTRPVVIIGGAIFAALMLLTSFTTKLWHVYVMFFFQAFFIGLAYQPTVTVIGFYFQKRLAFANGVTYSGVGVGIIIIPLMVEFLSSRFTWKGALQVMSAITLLVCFNGLLFRPSDKEKYFLLQKETARKNRISNKNCFERLKHAIASIPKFLGFHLLRENPRFITVTASCCVMGYGYYGSLVFFSSKAVFEAGLSTFQGALLLSIIGLGSTISRATHGILLDWKLISPMSLYALACFISASGNFLVPFAFSMNSLLVIAITFGLSSGLIFAVSIMCVRVVLREDQVAKGFGIIMMFNGLGTLVGIFFMGFLQRTTGSYSIPFVVAGAALALSGILMASDAIYVTYCKGPSPQNGTTNANVLREAESDNDPQEGETLTTTSGNTDSVRLKSLKTGETGFKEEVRLIGLNACDGEDVETSPVPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BYW8</accession>
    <name>A0A9Q1BYW8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF4371 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_22751</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BYW8"/>
    <dbReference type="OrthoDB" id="8950845at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="128"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8035501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="182" mass="21057" checksum="4A48251FA81802CE" modified="2023-09-13" version="1">MERTTLAYKLKEGVAEVAHQRTVKDLKETPFSINLDECTNRANNKVLTVLVSYFSESQGPRVEQSFSIMNDVICPKTNRLQVSTFGAIQTVKYDLQASHQTSIERYYREDRLKSPVDKHLCHHMQTSYSQHKQKQEAKPKAKLQINPTATKTKSTTNVHKVAKRIIQQQKAREEVLRKKRCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BZU8</accession>
    <name>A0A9Q1BZU8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein NATD1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">N-acetyltransferase domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_19385</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NATD1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8035645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BZU8"/>
    <dbReference type="OrthoDB" id="74247at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045057">
        <property type="entry name" value="Gcn5-rel_NAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031165">
        <property type="entry name" value="GNAT_YJDJ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31435">
        <property type="entry name" value="PROTEIN NATD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31435:SF9">
        <property type="entry name" value="PROTEIN NATD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14542">
        <property type="entry name" value="Acetyltransf_CG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51729">
        <property type="entry name" value="GNAT_YJDJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="4">
        <location>
            <begin position="40"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00031876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8035645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="132" mass="15202" checksum="AA9D5A88D1B625EC" modified="2023-09-13" version="1">MISSTFVCTVQIGRRSLHTQFSRLLTSVQQTKMGENFHVVHDTQKKEFTLKLEGSSEPAIIEYTERSDNSVDLHHTEVPVSYRGRGIAKVLAKNALDYFVSNQVKMRLSCTYLQKYVKDNPNPTYLQYLVEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C1P8</accession>
    <name>A0A9Q1C1P8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Coiled-coil domain-containing protein 57</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_18369</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C1P8"/>
    <dbReference type="OrthoDB" id="568502at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034451">
        <property type="term" value="C:centriolar satellite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005876">
        <property type="term" value="C:spindle microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007099">
        <property type="term" value="P:centriole replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007020">
        <property type="term" value="P:microtubule nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045931">
        <property type="term" value="P:positive regulation of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042481">
        <property type="entry name" value="CCDC57"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46725">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46725:SF1">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="800"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="13"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="120"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="520"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="800"/>
            <end position="818"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8037533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="964" mass="111900" checksum="E840CA2DF2C381BD" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C1Q5</accession>
    <name>A0A9Q1C1Q5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 4">Vacuolar protein sorting-associated protein 16 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_20514</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Believed to act as a core component of the putative HOPS and CORVET endosomal tethering complexes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Core component of at least two putative endosomal tethering complexes, the homotypic fusion and vacuole protein sorting (HOPS) complex and the class C core vacuole/endosome tethering (CORVET) complex. Their common core is composed of the class C Vps proteins VPS11, VPS16, VPS18 and VPS33A, which in HOPS further associates with VPS39 and VPS41 and in CORVET with VPS8 and TGFBRAP1. Interacts with RAB5C. Interacts with STX17, MON1B. Associates with adapter protein complex 3 (AP-3) and clathrin:AP-3 complexes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Late endosome membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
            <orientation evidence="4">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Lysosome membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
            <orientation evidence="4">Cytoplasmic side</orientation>
        </subcellularLocation>
        <text evidence="4">Cytoplasmic, peripheral membrane protein associated with late endosomes/lysosomes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the VPS16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C1Q5"/>
    <dbReference type="OrthoDB" id="1792at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033263">
        <property type="term" value="C:CORVET complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030897">
        <property type="term" value="C:HOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042144">
        <property type="term" value="P:vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.780:FF:000001">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 16 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.780">
        <property type="entry name" value="Vps16, C-terminal region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016534">
        <property type="entry name" value="VPS16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006925">
        <property type="entry name" value="Vps16_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038132">
        <property type="entry name" value="Vps16_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006926">
        <property type="entry name" value="Vps16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12811">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING VPS16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12811:SF0">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 16 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04840">
        <property type="entry name" value="Vps16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04841">
        <property type="entry name" value="Vps16_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF007949">
        <property type="entry name" value="VPS16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="4" id="KW-0458">Lysosome</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Vps16 N-terminal" evidence="6">
        <location>
            <begin position="5"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="Vps16 C-terminal" evidence="5">
        <location>
            <begin position="507"/>
            <end position="822"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00061859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR007949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8036514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="826" mass="94116" checksum="9F01C93F45D6F5D4" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C405</accession>
    <name>A0A9Q1C405_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_15572</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C405"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8038222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="83" mass="9391" checksum="EF33FA5EE170C7C8" modified="2023-09-13" version="1">MTILSFDGTTRLLPENCIYFARVGSHPPALPLPTSSFELLAHSVALRYTYGRLRVPHACRISDRSERRMHCLGIPVALFCLNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C4C1</accession>
    <name>A0A9Q1C4C1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">E3 ubiquitin-protein ligase TRIM56</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_19150</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8038152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C4C1"/>
    <dbReference type="OrthoDB" id="261644at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047153">
        <property type="entry name" value="TRIM45/56/19-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25462">
        <property type="entry name" value="BONUS, ISOFORM C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25462:SF296">
        <property type="entry name" value="MEIOTIC P26, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101898">
        <property type="entry name" value="NHL repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="17"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="7">
        <location>
            <begin position="93"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="202"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="264"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="330"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8038152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="723" mass="82790" checksum="337C121A8C419253" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C4D8</accession>
    <name>A0A9Q1C4D8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Plancitoxin-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_16655</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNase II family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C4D8"/>
    <dbReference type="OrthoDB" id="10261598at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004531">
        <property type="term" value="F:deoxyribonuclease II activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006309">
        <property type="term" value="P:apoptotic DNA fragmentation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004947">
        <property type="entry name" value="DNase_II"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10858">
        <property type="entry name" value="DEOXYRIBONUCLEASE II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10858:SF23">
        <property type="entry name" value="DEOXYRIBONUCLEASE II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03265">
        <property type="entry name" value="DNase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8039063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="127" mass="14437" checksum="E87A40750F7D1B16" modified="2023-09-13" version="1">MKGQYQPVAQTLQQIYNQPQMIAYIMYSDHPPDGKEKHPWGHTKGVVAYDESNGFWMIHSLPKFPRKDRYKWAENASLYGHGILCVSFKPLGKHREALSFAVKVVSSTSDQGARRIGSEMAGRLVKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C5D4</accession>
    <name>A0A9Q1C5D4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_16186</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C5D4"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8038692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="88" mass="9847" checksum="35987BC5ED6668BD" modified="2023-09-13" version="1">MPHIERKNPIVFGRGERSPGVTRGQTLKTLLTQYLKMRYGERKNPIIFGGGERSSGVTGGQTLKTLLTRYLKKGSSDGSQTWYIDAPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C604</accession>
    <name>A0A9Q1C604_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_16365</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C604"/>
    <dbReference type="OrthoDB" id="10062692at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8038825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="54" mass="6368" checksum="0FDDF36A35780FD8" modified="2023-09-13" version="1">MDRWVEHYSELYSTENTVSEEVINSIPALPSLHELDEEPTIAELEKSHQWLGKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C9T6</accession>
    <name>A0A9Q1C9T6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Neogenin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_15210</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C9T6"/>
    <dbReference type="OrthoDB" id="9944680at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004896">
        <property type="term" value="F:cytokine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050650">
        <property type="entry name" value="Type-II_Cytokine-TF_Rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20859:SF84">
        <property type="entry name" value="INTERFERON ALPHA_BETA RECEPTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20859">
        <property type="entry name" value="INTERFERON/INTERLEUKIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01108">
        <property type="entry name" value="Tissue_fac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040154474" evidence="3">
        <location>
            <begin position="19"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="458"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="4">
        <location>
            <begin position="357"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="546"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="559"/>
            <end position="574"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8040808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="574" mass="65223" checksum="FE38CBF5249A8F33" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C9Z6</accession>
    <name>A0A9Q1C9Z6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Coiled-coil domain-containing protein 78</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_15191</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C9Z6"/>
    <dbReference type="OrthoDB" id="2113965at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039873">
        <property type="entry name" value="CCDC78"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029329">
        <property type="entry name" value="DUF4472"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22106">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22106:SF5">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14739">
        <property type="entry name" value="DUF4472"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="5">
        <location>
            <begin position="1"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="394"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="601"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="783"/>
            <end position="841"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8040791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="902" mass="103587" checksum="E98984CC66265471" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CB51</accession>
    <name>A0A9Q1CB51_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_12274</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CB51"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8041454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="670" mass="76541" checksum="5B293A7C3B1DB023" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CBT0</accession>
    <name>A0A9Q1CBT0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Cystine/glutamate transporter</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Amino acid transport system xc-</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Solute carrier family 7 member 11</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">xCT</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">HOLleu_13025</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">Nanhai2018</strain>
            <tissue evidence="21">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-cystine(out) + L-glutamate(in) = L-cystine(in) + L-glutamate(out)</text>
            <dbReference type="Rhea" id="RHEA:70995"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:35491"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>N-acetyl-L-cysteine(out) + L-glutamate(in) = N-acetyl-L-cysteine(in) + L-glutamate(out)</text>
            <dbReference type="Rhea" id="RHEA:74567"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:78236"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>an L-alpha-amino acid(in) + L-kynurenine(out) = an L-alpha-amino acid(out) + L-kynurenine(in)</text>
            <dbReference type="Rhea" id="RHEA:71191"/>
            <dbReference type="ChEBI" id="CHEBI:57959"/>
            <dbReference type="ChEBI" id="CHEBI:59869"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="15">Disulfide-linked heterodimer with the amino acid transport protein SLC3A2/4F2hc; this interaction mediates cell membrane localization.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Microvillus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8042055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CBT0"/>
    <dbReference type="OrthoDB" id="10062876at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031528">
        <property type="term" value="C:microvillus membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015813">
        <property type="term" value="P:L-glutamate transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043067">
        <property type="term" value="P:regulation of programmed cell death"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000027">
        <property type="entry name" value="cystine/glutamate transporter isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050598">
        <property type="entry name" value="AminoAcid_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785:SF375">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="48"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="84"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="110"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="141"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="166"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="244"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="289"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="338"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="368"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="398"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="424"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00050407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00051652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00051885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00065438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00071314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00078584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00079117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00080978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAJ8042055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="480" mass="53236" checksum="6012B092A33E5317" modified="2023-09-13" version="1">MGSPSENSKVKLVKQVNLVDCIALVVGVIIGSGIFVSPKGILENTGTVGWALTVWVLCGVFTCVGALCYAELGTAIDKSGGDYTYLRIFHPILGFLRVWTMILAVRTVPWTVLSITAARYITASIGSFVCEDGVPKETTQILAGLILACIIYINCWSVQLSRRLQIFFTIAKTVGLTIIIICGMWALLKGHTGSFQNAFNPEEVKWVQFPKAFYAGLFAYSGWQYLPQVTEEIINPGRNVPLSIIISVVIVMVVYILTNVAYFAVLSPFELLASPAVAVTFGQRVLGPVWWVMPVFVAMSCIGSINGGIFGTARMFFVASREGQLPEILGMIHIRRKTPLPAASFALPICLLMLITDDVYALINYLSFSRWLFIAITVALVPYFRWKYPDLDRPFKVPLVLPVLFTFCALFMVISSILSAATEFAIGFVITFAGVPVYLLLVWWDSKPKCFTDAMRNMTIFLQKLLVVIHQEEKTYTNEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CCD0</accession>
    <name>A0A9Q1CCD0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">G1/S-specific cyclin-E</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_12926</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cyclin family. Cyclin E subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CCD0"/>
    <dbReference type="OrthoDB" id="5590282at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20519">
        <property type="entry name" value="CYCLIN_CCNE_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20520">
        <property type="entry name" value="CYCLIN_CCNE_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000024">
        <property type="entry name" value="G1/S-specific cyclin-E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039361">
        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004367">
        <property type="entry name" value="Cyclin_C-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048258">
        <property type="entry name" value="Cyclins_cyclin-box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02984">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01332">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00292">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="5 8" id="KW-0195">Cyclin</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="9">
        <location>
            <begin position="117"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin C-terminal" evidence="10">
        <location>
            <begin position="211"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8041974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="363" mass="41875" checksum="DA45E115CEF8D65B" modified="2023-09-13" version="1">MYIGRINVVLFILSQQNLWIPVSENSGFNRSALIPTPESEPASPIGQPVDSFSSFRFSNLFPCPLRDRKSPLPKLNWADSNELWKNMLKKEVHYARVSNCLEKHPALDAKMRSILLDWLIEVCEVYHLHRETFFLAQDYVDRYLAETSNVPKMQLQLIGITALFIASKLEEIYPPKLGDFSYVTDGACTDENILDQEILMLKALKWALSPVTVNTWLNVYMQLGQTEEIVQEGSNFLIPKYSPQEFIQITQLLDLCILDEGSWQFSYSTIAATALHHMSSNNLALEVTGLKWDDIAMCVQWMEPFALTIKDLDRVVELRKFRRVMPEDCHNIQTHADNLQLLVSESYWIHHCFRSLMSKIITL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CF14</accession>
    <name>A0A9Q1CF14_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Leucine-rich repeat-containing protein 43</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_11285</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CF14"/>
    <dbReference type="OrthoDB" id="433501at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15454:SF19">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15454">
        <property type="entry name" value="NISCHARIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14580">
        <property type="entry name" value="LRR_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52075">
        <property type="entry name" value="Outer arm dynein light chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="332"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="475"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="565"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="332"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="350"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="493"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="589"/>
            <end position="616"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8043957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="668" mass="74707" checksum="182B8D1A73AE6F8F" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CF61</accession>
    <name>A0A9Q1CF61_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">EamA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_10771</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CF61"/>
    <dbReference type="OrthoDB" id="306876at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22911">
        <property type="entry name" value="ACYL-MALONYL CONDENSING ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22911:SF6">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER G1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="15"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="45"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="75"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="100"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="135"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="166"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="200"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="226"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="252"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="6">
        <location>
            <begin position="7"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8043598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="288" mass="31563" checksum="874222309DC66B7B" modified="2023-09-13" version="1">MLIHLSATRMNPLNVVFWNAVCANCIFVVAVCIERPRTTGSIKEILILISSGFFGGCGSITFSIAVMITREPGDAVALYFTLPISVIILEVFFHQGKVKMAYVIFAFLSFAGVIFVVKPSFLFHNRTNDNATNTLGAVLSLSSSFLMVAQFIAVSKLKIFATNFSLVVFLTNSFVLSISFMLCVYLRVFKVPDSVEKVMFILFTGCLYVSLYSLVYLAVTNERPVVVSVIMTSEVAFTFIAQSVALSLTTTWNSVVGGILISLSCLGVSLAKQADEENPENEDLLQEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CF75</accession>
    <name>A0A9Q1CF75_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">14-3-3 protein epsilon</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_11423</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the 14-3-3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8044062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CF75"/>
    <dbReference type="OrthoDB" id="10260625at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.190.20">
        <property type="entry name" value="14-3-3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000308">
        <property type="entry name" value="14-3-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023409">
        <property type="entry name" value="14-3-3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036815">
        <property type="entry name" value="14-3-3_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023410">
        <property type="entry name" value="14-3-3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18860">
        <property type="entry name" value="14-3-3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00244">
        <property type="entry name" value="14-3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000868">
        <property type="entry name" value="14-3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00305">
        <property type="entry name" value="1433ZETA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00101">
        <property type="entry name" value="14_3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48445">
        <property type="entry name" value="14-3-3 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00796">
        <property type="entry name" value="1433_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00797">
        <property type="entry name" value="1433_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="14-3-3" evidence="5">
        <location>
            <begin position="1"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="213"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="229"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with phosphoserine on interacting protein" evidence="2">
        <location>
            <position position="35"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with phosphoserine on interacting protein" evidence="2">
        <location>
            <position position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000868-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8044062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="238" mass="26919" checksum="62CB4859743E6745" modified="2023-09-13" version="1">MVEAMKRVAAMDVDLSVEERNLLSVAYKNVIGARRASWRIISNMESKAEAKGDKAEIPRIVQYRKEVEKELSDICNDVLEVIDKHLMGAAISKESQVFYHKMKGDYYRYLAEFSGGDARKEAAEKSLDAYKLASETAIVDLEPTNPIRLGLALNFSVFYYEILNSPERACRLAKAAFDDAIAELDALNESSYKDSTLIMQLLRDNLTLWTSDIPKEDEDQDGNAKLDVEDIENFEENS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CFF4</accession>
    <name>A0A9Q1CFF4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Coronin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_11269</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the WD repeat coronin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CFF4"/>
    <dbReference type="OrthoDB" id="1850764at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000502">
        <property type="entry name" value="Coronin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015505">
        <property type="entry name" value="Coronin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015048">
        <property type="entry name" value="DUF1899"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10856">
        <property type="entry name" value="CORONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10856:SF0">
        <property type="entry name" value="CORONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08953">
        <property type="entry name" value="DUF1899"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16300">
        <property type="entry name" value="WD40_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01166">
        <property type="entry name" value="DUF1899"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01167">
        <property type="entry name" value="DUF1900"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="4 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 6 7" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="DUF1899" evidence="10">
        <location>
            <begin position="2"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="75"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="125"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="168"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="411"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="568"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="430"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="471"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="499"/>
            <end position="508"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU280818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8043945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="617" mass="68136" checksum="7B797BC541342B71" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CG96</accession>
    <name>A0A9Q1CG96_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Proline dehydrogenase</fullName>
            <ecNumber evidence="8">1.5.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_11517</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Converts proline to delta-1-pyrroline-5-carboxylate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-proline + a quinone = (S)-1-pyrroline-5-carboxylate + a quinol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23784"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17388"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.5.5.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>trans-4-hydroxy-L-proline + a quinone = (3R,5S)-1-pyrroline-3-hydroxy-5-carboxylate + a quinol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52512"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:58375"/>
            <dbReference type="ChEBI" id="CHEBI:62612"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.5.5.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the proline oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.5.5.2" evidence="8"/>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8044135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CG96"/>
    <dbReference type="OrthoDB" id="5464at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004657">
        <property type="term" value="F:proline dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010133">
        <property type="term" value="P:L-proline catabolic process to L-glutamate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029041">
        <property type="entry name" value="FAD-linked_oxidoreductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002872">
        <property type="entry name" value="Proline_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015659">
        <property type="entry name" value="Proline_oxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13914:SF29">
        <property type="entry name" value="HYDROXYPROLINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13914">
        <property type="entry name" value="PROLINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01619">
        <property type="entry name" value="Pro_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51730">
        <property type="entry name" value="FAD-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0274">FAD</keyword>
    <keyword evidence="3 8" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6 8" id="KW-0642">Proline metabolism</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proline dehydrogenase" evidence="9">
        <location>
            <begin position="270"/>
            <end position="531"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8044135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="554" mass="62974" checksum="E1DD2BBC2A1A15F9" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-06-18" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CGM7</accession>
    <name>A0A9Q1CGM7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Stonustoxin subunit alpha</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_07868</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2386367at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00882">
        <property type="entry name" value="Ras_like_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052090">
        <property type="entry name" value="Cytolytic_pore-forming_toxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030379">
        <property type="entry name" value="G_SEPTIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056072">
        <property type="entry name" value="SNTX_MACPF/CDC-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048997">
        <property type="entry name" value="Stonustoxin-like_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040581">
        <property type="entry name" value="Thioredoxin_11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31594">
        <property type="entry name" value="AIG1-TYPE G DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31594:SF14">
        <property type="entry name" value="FIBRONECTIN TYPE-III DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24674">
        <property type="entry name" value="MACPF_SNTX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00735">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21109">
        <property type="entry name" value="Stonustoxin_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18078">
        <property type="entry name" value="Thioredoxin_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="3">
        <location>
            <begin position="562"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="3">
        <location>
            <begin position="658"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="406"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="997"/>
            <end position="1018"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="997"/>
            <end position="1011"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU004560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8044962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1327" mass="149569" checksum="DD5000B37F19F9A3" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CI61</accession>
    <name>A0A9Q1CI61_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="10">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_08870</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">Interacts with ARFGEF1/BIG1 and the C-terminal of EPB41L2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the FKBP-type PPIase family. FKBP2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="10"/>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CI61"/>
    <dbReference type="OrthoDB" id="77911at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.50.40:FF:000016">
        <property type="entry name" value="Peptidylprolyl isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044609">
        <property type="entry name" value="FKBP2/11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45779">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45779:SF7">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 10 13" id="KW-0413">Isomerase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0697">Rotamase</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040437079" description="peptidylprolyl isomerase" evidence="11">
        <location>
            <begin position="26"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase FKBP-type" evidence="12">
        <location>
            <begin position="49"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00064679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8045787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="142" mass="15923" checksum="1C47AE75AE501BA1" modified="2023-09-13" version="1">MTMQMRQVCFTFIVVLMIVITEVNSEKAKKLQIGVKKKVENCSRRSRNGDRLSMHYTGTLENGEEFDSSIPRGEPFVFTLGSGQVIKGWEQGLLNMCEGEKRKLVIPSDLGYGDRGAPPKIPGGSTLIFEVELIKIERNTEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CIX4</accession>
    <name>A0A9Q1CIX4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NADH-ubiquinone oxidoreductase chain 6</fullName>
            <ecNumber evidence="3">7.1.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">NADH dehydrogenase subunit 6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HOLleu_04733</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Nanhai2018</strain>
            <tissue evidence="17">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a ubiquinone + NADH + 5 H(+)(in) = a ubiquinol + NAD(+) + 4 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29091"/>
            <dbReference type="Rhea" id="RHEA-COMP:9565"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="7.1.1.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the complex I subunit 6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.2" evidence="3"/>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CIX4"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1200">
        <property type="entry name" value="NADH-ubiquinone/plastoquinone oxidoreductase chain 6, subunit NuoJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050269">
        <property type="entry name" value="ComplexI_Subunit6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001457">
        <property type="entry name" value="NADH_UbQ/plastoQ_OxRdtase_su6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042106">
        <property type="entry name" value="Nuo/plastoQ_OxRdtase_6_NuoJ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11435">
        <property type="entry name" value="NADH UBIQUINONE OXIDOREDUCTASE SUBUNIT ND6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11435:SF1">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00499">
        <property type="entry name" value="Oxidored_q3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0249">Electron transport</keyword>
    <keyword evidence="13 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-0520">NAD</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="8" id="KW-1278">Translocase</keyword>
    <keyword evidence="7 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="48"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="82"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00031019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ8046151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="151" mass="16637" checksum="C6495E7A0D1CA142" modified="2023-09-13" version="1">MVFYLFLLLLLLGRTLVFYRLSPYYAALGLVLTSLFGCIILRCLGLSFLALLLLLVYMGGMMVVFVYSSALSADRYPVVRNLGEVFVVFILLSAWVFLIFEDFSEFGLRGEKILSLGDLDRLSSLYSAGGLYLVIGGIALLVVLVVALVVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CK08</accession>
    <name>A0A9Q1CK08_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Cholesterol 24-hydroxylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_09124</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CK08"/>
    <dbReference type="OrthoDB" id="1470350at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033781">
        <property type="term" value="F:cholesterol 24-hydroxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006707">
        <property type="term" value="P:cholesterol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20613">
        <property type="entry name" value="CYP46A1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039983">
        <property type="entry name" value="CYP46A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24293:SF0">
        <property type="entry name" value="CYP46A1 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24293">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 46 SUBFAMILY A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0349">Heme</keyword>
    <keyword evidence="2 3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="2">
        <location>
            <position position="445"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8045984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="498" mass="57473" checksum="74470C4133763F27" modified="2023-09-13" version="1">MMLTIILLKYTALVVLAVVLGAFLAACVFLYRLHSRYDHLPGPKRTNFFTGNASEFRSAVENQTPFIHRMLELQQEFGPVNVIFFFHQAFLSVADPATVKALLMDDVHTKPDTYKMLYEVYGVRFMGKGLVTEQDHSKWYNHRKIFNPAFHRKHLMGLMSYFSISSDKLVIKLKDDVDTGKAVSLMDGFSRTTLDVIAKAGIGMTQELILDESPFVEAVITSLKGMLNKYQKPYDLIFPSHWKFRREVQRAVQCLRDTGKELISRSRKALLMGEDLPNNILGNVMKYSNPSEDQFDMETMVDEFLTFFVAGQESTSNLLAFTMVQLGRHPEILKRVRDEIDAVVGDKDFIEYEDVMKMEYTLLVLKETLRLYPPVTGTVRRLAYDMIVCGHNVPAGSNVQMSHYIMARMEQFFKNPLTFDPERFLRDEDKPLYTYFPFSLGTRSCIGQQFALIEARVILAKLYQNYDFKLDPTQSFEILDQLTLKPKGQCMNYIYSRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CKD6</accession>
    <name>A0A9Q1CKD6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Serine/threonine-protein kinase TBK1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_06041</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CKD6"/>
    <dbReference type="OrthoDB" id="10013850at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010628">
        <property type="term" value="P:positive regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045089">
        <property type="term" value="P:positive regulation of innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009967">
        <property type="term" value="P:positive regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd12219">
        <property type="entry name" value="Ubl_TBK1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000100">
        <property type="entry name" value="inhibitor of nuclear factor kappa-B kinase subunit epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000106">
        <property type="entry name" value="serine/threonine-protein kinase TBK1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051180">
        <property type="entry name" value="IKK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041309">
        <property type="entry name" value="TBK1_CC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041087">
        <property type="entry name" value="TBK1_ULD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22969:SF15">
        <property type="entry name" value="FI05319P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22969">
        <property type="entry name" value="IKB KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18394">
        <property type="entry name" value="TBK1_CCD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18396">
        <property type="entry name" value="TBK1_ULD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="16"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8047132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="812" mass="92873" checksum="603FEB84DC34ADA3" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CLC2</accession>
    <name>A0A9Q1CLC2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Carboxylesterase type B domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_05913</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CLC2"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8047031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="95" mass="11049" checksum="26BA47221FDE38E7" modified="2023-09-13" version="1">MGQKFTTDEETLIDIYSEFLANFIHYGDPNTISDNVKPELANLNLVPWKPIDPRDLQTMVFSTTETLECKIGIRQKQCDLLDDIGYYNVPYMNRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CLW0</accession>
    <name>A0A9Q1CLW0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_05577</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CLW0"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8046789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="72" mass="8373" checksum="6CC29041B98523E0" modified="2023-09-13" version="1">MWMCYGEKMDPIVFGEGQRPSGVTRGKKAKFPKNSQEFYMGSAEVKEQNFPRFPKIVIWGHQRSNCKFPKNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CPF2</accession>
    <name>A0A9Q1CPF2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_01488</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CPF2"/>
    <dbReference type="OrthoDB" id="8046937at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="126"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8048963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="287" mass="31764" checksum="AC0A83FE697E92CE" modified="2023-09-13" version="1">MHLKSTRVRATKRASLTRLGKEIKALITENPHPNDECKAIVQSKGEAYNQAVLDLEEAHKAYLVALKDEDDIHEAEAYMAAIHLTVNDIRQVSSMWQNSQDEMMVKPSDSISQAGSGSSVTSERAKANAKRAVSAAKAALLKQQQELQSAELKLKQQRDELDLKMAIAMAEAEEKAYIEAEGNGVDLSMGKAKVDVEKPSSVSNGVKLCPEAPAFQPREEIAIPKHEAIPQPLEFSQNHLIEAVHLPRVELSYFDGNPMKYWSFIRSFENSVGRSNLSDGAKLTRLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CUH5</accession>
    <name>A0A9Q1CUH5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Histidine--tRNA ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_04431</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8051019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CUH5"/>
    <dbReference type="OrthoDB" id="8718740at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040439284" evidence="2">
        <location>
            <begin position="26"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="624"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="654"/>
            <end position="681"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8051019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="710" mass="81372" checksum="E5AA3A9AEB6D0839" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H1U3</accession>
    <name>A0A9Q1H1U3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_25405</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H1U3"/>
    <dbReference type="OrthoDB" id="6118220at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061343">
        <property type="term" value="P:cell adhesion involved in heart morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007508">
        <property type="term" value="P:larval heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33395:SF22">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33395">
        <property type="entry name" value="TRANSCRIPTASE, PUTATIVE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14529">
        <property type="entry name" value="Exo_endo_phos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="1">
        <location>
            <begin position="94"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8032007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="172" mass="19557" checksum="DF42547652FBD716" modified="2023-09-13" version="1">MSYLEVHVSLLEPDFFGITETWAKEDMHPDSELMISGYTMYRIDRKHRTKMRGGGCILYVKNCFNCVLRDDLTNQDGVDSVWCNLCTSHGSKLLIGLCYRSPSNSETENQALYNLIRVASTEQVIIMGDFNHPSIDWDNLCANTADSAFLDLIQDCFLTQHVNEPTHKLGNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H226</accession>
    <name>A0A9Q1H226_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Insulin-like growth factor-binding protein complex acid labile subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_28597</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H226"/>
    <dbReference type="OrthoDB" id="676979at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001164">
        <property type="entry name" value="GH01279p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026906">
        <property type="entry name" value="LRR_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050333">
        <property type="entry name" value="SLRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45712">
        <property type="entry name" value="AGAP008170-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45712:SF22">
        <property type="entry name" value="INSULIN-LIKE GROWTH FACTOR-BINDING PROTEIN COMPLEX ACID LABILE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13306">
        <property type="entry name" value="LRR_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8029251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="539" mass="61778" checksum="9859450ED346B983" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H2C6</accession>
    <name>A0A9Q1H2C6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">E3 ubiquitin-protein ligase TRIM56</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_26209</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H2C6"/>
    <dbReference type="OrthoDB" id="654191at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047153">
        <property type="entry name" value="TRIM45/56/19-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25462">
        <property type="entry name" value="BONUS, ISOFORM C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25462:SF296">
        <property type="entry name" value="MEIOTIC P26, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101898">
        <property type="entry name" value="NHL repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="17"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8032637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="392" mass="44006" checksum="DCDB766FCD8F95C6" modified="2023-09-13" version="1">MATSSPLKDIGDKFCLCSICLEQLKEPKLLPCLHRYCKDCLNSIIQGTYDVIQCPDCRQETQIPTNGVDGFKTDFYSKNLVEYVQIQQSLKSDITVNYKQYSISKTSVTKISENFDTKISIYDPNRYVCSITSIGDGNIVISGYTSDLKASFMIVIDMNGRMLKEKILNTGEILPVRFCKFLSQHKVASVCTPNDIGLYDVRDGSYIKKNISDVISSWPKGRDVSCVATNPVNNHILVGGRNSTDVYVFDDQLNYLHILTLPEMIKRPHDITVSDGHLLVCDNDGEKCFVTTMDGSESKVVGEFMKPNLEGYMFGPTSVYSDKNGLVYVLWKSSPQCYIVQYNHDGSQVLTTRMLDVDAHVVTVVETSQGEKLLVATCDTRTVYLYNLMTED</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H2P4</accession>
    <name>A0A9Q1H2P4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tick transposon</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_29524</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H2P4"/>
    <dbReference type="OrthoDB" id="775972at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33198">
        <property type="entry name" value="ANK_REP_REGION DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33198:SF19">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8029971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="176" mass="20210" checksum="87A1C55FB25F50B7" modified="2023-09-13" version="1">MARSSHLKVESYDPEVEKWESYEERLTMFFEANNVTEDKKKVAIFLSSMGAKGYGLLKNLVSPDKPSAKNFPEICKTLKDYYSPRPPVLLERFRFYSHTQSASESVTNFLAELKRLSSTCNFGAFLKDALRDRFVCGLYDATVKKKAPIGRRLLNFRKSAENCRQYGNSSQEFSDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H3F2</accession>
    <name>A0A9Q1H3F2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_24256</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H3F2"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8033882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="118" mass="14032" checksum="6D5892F3CA159264" modified="2023-09-13" version="1">MERSDYLVECYRQLADQTFYRKLDWDPTQGLSMTLRDWCRVYSMNASLQVICVISLFQRIHALVDFIFCLSCIKWAIPVDLSFLAVVILQKIYLFTLTFSYNPLPNLYLLTLRTLRIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H3G9</accession>
    <name>A0A9Q1H3G9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">SPRY domain-containing SOCS box protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_24331</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H3G9"/>
    <dbReference type="OrthoDB" id="5951542at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050672">
        <property type="entry name" value="FBXO45-Fsn/SPSB_families"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12245">
        <property type="entry name" value="SPRY DOMAIN CONTAINING SOCS BOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12245:SF12">
        <property type="entry name" value="SPRY DOMAIN-CONTAINING SOCS BOX PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="141"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8033942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="168" mass="18686" checksum="10528C1233372F06" modified="2023-09-13" version="1">MAGLTEMEGGCFHDWSWNSNDKSHEIFLSGRRNEHVKFHPSWSVGTAAVRGTKPLTRGCKTLWEISLKQPFYGTSIMFGVGTKRARIHADSFVNLLGEDGESMGLSHKGLIWQKGVANKGTGRFDFFVSSLNSAKSCYRRSGKNGKAVTTEARRREKAGQDVTQDKSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H3V0</accession>
    <name>A0A9Q1H3V0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_21178</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H3V0"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8034377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="84" mass="9462" checksum="AFED7D356AFB83D7" modified="2023-09-13" version="1">MYVCMYVCMYGCTQQHVLNRTRYSRRELRSRTSFLAHAELETGVAARKFRRCDEKSRAAARACLGKQPAFQTRTSAGLVGGQPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H442</accession>
    <name>A0A9Q1H442_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_25604</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H442"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8032158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="143" mass="15924" checksum="B39EBF28DC917687" modified="2023-09-13" version="1">MNRMSEYDTESYCCCSLRDRCLIFAAQIMGLFAVADVGFFAVAVIINSPEFQMITESMIKPVYGDGITSYFIGAFVLCSVYVIVAVCLIAGILKKRRWLLLVFLIAFGIFVGLQSLGTLSTMISLVSIFFLLYCKYNSHITST</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H5I7</accession>
    <name>A0A9Q1H5I7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_23280</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H5I7"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040322233" evidence="2">
        <location>
            <begin position="26"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="30"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8033136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="94" mass="9216" checksum="D98907DF073064FA" modified="2023-09-13" version="1">MKKSTSIVLIAIVLLASQLVLDIEASNSESSSSPSSPPSSTSSSPGGPTPPPTTTAPPTTTAPPTTTPPPTTTPPPTTTPPPPPPTPPKAKTAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H6B7</accession>
    <name>A0A9Q1H6B7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_21210</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H6B7"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8034393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="70" mass="7586" checksum="1A618445A271C000" modified="2023-09-13" version="1">MAKRPPLSVQVQVTLYVCIPLKKLPDCWSQVVIRLTSACSVSVQVGSLHTAKLPILLSRDVTVTVSGQII</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H6C3</accession>
    <name>A0A9Q1H6C3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_24487</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8034065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H6C3"/>
    <dbReference type="OrthoDB" id="10062678at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051077">
        <property type="entry name" value="Ca-dependent_lectin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24024">
        <property type="entry name" value="PULMONARY SURFACTANT-ASSOCIATED PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24024:SF18">
        <property type="entry name" value="SHORT-CHAIN COLLAGEN C4-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8034065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="107" mass="11594" checksum="5085093215263F20" modified="2023-09-13" version="1">MDVPCAVCKAPSRFGQLMIPGKNSCPSAEWTLEYSGYLMSERNHPAHLRAMYICVDREMQSVGGSHGPSTSRGVIRFVESRCSWGGGGLPCGPYVDGYELTCAVCTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H7J5</accession>
    <name>A0A9Q1H7J5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_22572</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H7J5"/>
    <dbReference type="OrthoDB" id="10035396at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8035370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="188" mass="21732" checksum="C5157CEA12D63AD2" modified="2023-09-13" version="1">MRIRKTTEPIQCHNCQRYGHYAASCQHRKTCLRCAGHHALADCPTPRDEPRCANCSRHHIASYKGCSRYQRALEEQKKKDAQKTRPAQGNRSVPAPRPDKPNSTSFCSPQTSDLQKKHDEAMKKIEERHQLELEAIRLQHQATIEKIEEANTQLFHQLREDTTTQINEMKGRIIHFLGDVLHHLIPTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H8R7</accession>
    <name>A0A9Q1H8R7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Extracellular serine/threonine protein kinase FAM20C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_17416</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAM20 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8036780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H8R7"/>
    <dbReference type="OrthoDB" id="8583677at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024869">
        <property type="entry name" value="FAM20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009581">
        <property type="entry name" value="FAM20_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12450">
        <property type="entry name" value="DENTIN MATRIX PROTEIN 4 PROTEIN FAM20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12450:SF22">
        <property type="entry name" value="EXTRACELLULAR SERINE_THREONINE PROTEIN CG31145"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06702">
        <property type="entry name" value="Fam20C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0464">Manganese</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="12" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="FAM20 C-terminal" evidence="11">
        <location>
            <begin position="269"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="491"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="491"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="377"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="397"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="397"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR624869-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR624869-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR624869-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF06702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8036780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="517" mass="60555" checksum="57E28C98326ACF0E" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H8X0</accession>
    <name>A0A9Q1H8X0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Fizzy-related protein homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Cdh1/Hct1 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_14387</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat CDC20/Fizzy family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H8X0"/>
    <dbReference type="OrthoDB" id="10263272at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010997">
        <property type="term" value="F:anaphase-promoting complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990757">
        <property type="term" value="F:ubiquitin ligase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905786">
        <property type="term" value="P:positive regulation of anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000025">
        <property type="entry name" value="FIZZY-related 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033010">
        <property type="entry name" value="Cdc20/Fizzy"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056150">
        <property type="entry name" value="WD40_CDC20-Fz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19918">
        <property type="entry name" value="CELL DIVISION CYCLE 20 CDC20 FIZZY -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19918:SF1">
        <property type="entry name" value="FIZZY-RELATED PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24807">
        <property type="entry name" value="WD40_CDC20-Fz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 10" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="CDC20/Fizzy WD40" evidence="12">
        <location>
            <begin position="174"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="220"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="304"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="350"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="438"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="55"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00073600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00081406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF24807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8040167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="492" mass="54465" checksum="189BF543E10713FB" modified="2023-09-13" version="1">MDQEYEKRLLKQQNGQSPVGAQMPRVLSPGKAASPSKEQYGDRFIPSRAGNTWHTKFDSPKKKDATNSATKKDREGGDTGEDMLAYHCLLQNELLGAEISTVKDPQCEDRRMVSLQTAKRNIFSYSVKTQRPSLAVGQDEQPYSLSPVGKDSQKLLRSPRKPMRKISKIPFKVLDAPELQDDFYLNLVDWSSTNILSVGLGTCVYLWSACTSHVTRLCDLSVDVDSVTSVAWNERGLQVAVGTHKGLVQIWDATSQKKLVTLEGHSARVGALAWNGDSICSGSRDRMILQRDSRSPGHASTRRLSGHRQEVCGLKWSPDHQYLASGGNDNKVGVIAVSYSHSSFYPIQQYSDHTAAVKAIAWSPHQHGLLASGGGTADKYIRFWNTLTGQTLNAVDTGSQVCNLAWSKHDNELVSTHGYSQNQILVWKYPSLVQVAKLTGHTFRVLYLAVSPDGEAIVTGAGDETLRFWNVFSKSRSTKETKSVLNLFNHIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H9U7</accession>
    <name>A0A9Q1H9U7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_15732</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H9U7"/>
    <dbReference type="OrthoDB" id="1095242at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001753">
        <property type="entry name" value="Si:ch211-119o8.6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000130">
        <property type="entry name" value="Spalt-like transcription factor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000100">
        <property type="entry name" value="Zinc finger 45-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000358">
        <property type="entry name" value="zinc finger protein 24"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000193">
        <property type="entry name" value="Zinc finger protein 300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000110">
        <property type="entry name" value="Zinc finger protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24409:SF295">
        <property type="entry name" value="AZ2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24409">
        <property type="entry name" value="ZINC FINGER PROTEIN 142"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="15"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="59"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="93"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="121"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="149"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="205"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="233"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="261"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="289"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="317"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="345"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="373"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="400"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="428"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="455"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="483"/>
            <end position="506"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8038339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="506" mass="60017" checksum="DCFB2705F541B5A5" modified="2023-09-13" version="1">MTHTDEKTINCQFCEKSVMTERGCKIHGMIRTGEILLQSQFCNKTLNTKGDCKRHEKIHICEKPFNSKFCDMSFFTKTACKRHMMIHTNERPYKSQECEKSCITKNDYKRHKQRHTDERPFKCQYCDRSYVLKRELKIHEMTHTGKKPFKCDFCDQAFTTKNSCKIHMLIHTDERPFKCQYCDIPFRERGKWKSHEVRNSADGLYKCQFCDMSFQTKCICARHEMTHTGDRLFYCQFCNKSFKIKRNCKRHENIHLGKRPFCCQFCGKSFTTKSICKTHEMIHTGEKPFKCDFCDKSFHTKLVCARHEVTHTGERPFKCQFCDKAFQTKHVCAGHEMMHTGEGPFKCQFCKKSFATKGICTRHEKIHTGERPFKCQFCEKSFLWKESCKRHEMIHTGRPIKCQFCEKSFVTKGNCARHEKTHTGERPFKCQYCDKAFQTKCVCSRHEMTHTGRPIKCQFCEKSFVTKGNCAQHEMIHTGERPFKCWFCEKSFSRKGNCKRHEMIHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HAZ2</accession>
    <name>A0A9Q1HAZ2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Solute carrier family 35 member G1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_16468</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HAZ2"/>
    <dbReference type="OrthoDB" id="306876at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22911">
        <property type="entry name" value="ACYL-MALONYL CONDENSING ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22911:SF6">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER G1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="58"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="95"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="122"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="152"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="185"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="219"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="6">
        <location>
            <begin position="33"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8038908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="281" mass="30808" checksum="16F178A47678C7BB" modified="2023-09-13" version="1">MEKLDHDTEESKKESPPEAGVLHTVRTKVFEQRGLLAAFLASLCFALQTVWVKLMKDAMPVVEVVCIRFALQGMFSLMFGMFTKMSILPDSKREMALLAARGSLGTIAIGLIYFAVYNMSAGLVNTIVFGAPVFVGILAWIFLKERFYCCDAILMAVDILGIVLIAQPPFLFSSESWSDSKKMELFGAIAAFCAMLLAASTAILIRLMGLMGTSAIKIVFYYSIIGTLLPVIIGIIMGDWVLPQCGTMRFLLVGIGFMNFAAQSLSRMRLLSPSSHTSPQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HB07</accession>
    <name>A0A9Q1HB07_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_17009</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HB07"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8039330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="78" mass="8889" checksum="44D6682C78815860" modified="2023-09-13" version="1">MESSREFNFNAAFLELSRSENCLHGLTTLLRNIDTSPSLVVIRSWNQFLRYSTLQVYYCTIVNVHALPALGPDQANIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HBG1</accession>
    <name>A0A9Q1HBG1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_17007</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HBG1"/>
    <dbReference type="OrthoDB" id="10685610at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040392348" evidence="2">
        <location>
            <begin position="22"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="244"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8039328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="891" mass="101884" checksum="B23AC420E38164D8" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HBI5</accession>
    <name>A0A9Q1HBI5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein eS28</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">40S ribosomal protein S28</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_14184</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS28 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HBI5"/>
    <dbReference type="OrthoDB" id="10258930at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04457">
        <property type="entry name" value="S1_S28E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000025">
        <property type="entry name" value="40S ribosomal protein S28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00292">
        <property type="entry name" value="Ribosomal_eS28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000289">
        <property type="entry name" value="Ribosomal_eS28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028626">
        <property type="entry name" value="Ribosomal_eS28_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10769">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10769:SF3">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN ES28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01200">
        <property type="entry name" value="Ribosomal_S28e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00961">
        <property type="entry name" value="RIBOSOMAL_S28E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8040004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="66" mass="7507" checksum="8FC9CBFF7069B528" modified="2023-09-13" version="1">MDKPVKLAKQVTKVLGRTGSQGQCTQVRVEFLDDTNRSIIRNVKGPVREGDILTLLESEREARRLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HBW7</accession>
    <name>A0A9Q1HBW7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_17374</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HBW7"/>
    <dbReference type="OrthoDB" id="10059790at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF20">
        <property type="entry name" value="REVERSE TRANSCRIPTASE-LIKE PROTEIN WITH RNA-DIRECTED DNA POLYMERASE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8039601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="93" mass="10829" checksum="454EAFBB77AD693B" modified="2023-09-13" version="1">MRMNTRSDQKLKVNNENLEDVDYYTYLGSKIKKSEGGSEDTKHGLTKTLASFKKLSKIWYQMNVLRRTEILLYKSLVSSVLLYGCKTWKPNTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HBY5</accession>
    <name>A0A9Q1HBY5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Gem-associated protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_14749</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HBY5"/>
    <dbReference type="OrthoDB" id="10664049at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032797">
        <property type="term" value="C:SMN complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000387">
        <property type="term" value="P:spliceosomal snRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033265">
        <property type="entry name" value="GEMIN4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15571">
        <property type="entry name" value="GEM-ASSOCIATED PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15571:SF2">
        <property type="entry name" value="GEM-ASSOCIATED PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8040454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1008" mass="114569" checksum="38F872BBF2114DF0" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HCV3</accession>
    <name>A0A9Q1HCV3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">CRESS-DNA virus Rep endonuclease domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_07555</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HCV3"/>
    <dbReference type="OrthoDB" id="5986179at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1310.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049912">
        <property type="entry name" value="CRESS_DNA_REP"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52020">
        <property type="entry name" value="CRESS_DNA_REP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0190">Covalent protein-DNA linkage</keyword>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CRESS-DNA virus Rep endonuclease" evidence="11">
        <location>
            <begin position="8"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS52020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8044727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="134" mass="15201" checksum="BECC32B2C1FEE2AD" modified="2023-09-13" version="1">MASELGARQVRMVYLITYSQADSNVCGSREDFASKVLSAFRSSGIKVMHWVCSRENHQDGGHHYHMSVKLDQGRRWLRVKQMLEAEHNITVNFSSTHVNYYTAYKYVVKEEDNALYSPGHPDLGDCSPKSTVAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HD81</accession>
    <name>A0A9Q1HD81_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_15505</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8041023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HD81"/>
    <dbReference type="OrthoDB" id="5945173at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040197867" evidence="1">
        <location>
            <begin position="21"/>
            <end position="674"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8041023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="674" mass="72797" checksum="BCB0D2DE27E0C247" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HEU6</accession>
    <name>A0A9Q1HEU6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Integrase core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_05942</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HEU6"/>
    <dbReference type="OrthoDB" id="10045093at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058913">
        <property type="entry name" value="Integrase_dom_put"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46791:SF13">
        <property type="entry name" value="CLR5 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46791">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24764">
        <property type="entry name" value="rva_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase core" evidence="1">
        <location>
            <begin position="119"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF24764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8047052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="384" mass="45775" checksum="996A6480ED461C5C" modified="2023-09-13" version="1">MDQVNQFVRFYFHLGFSHKEIVFCLQQCHGVHLSLTTVRRRLKGMALYRRRKSDLLEVALFVMEELERHGQLHGYKTMHLKCIQKGLQVTQETVRILLQLLDPEGVAYRRSKRLRRRLYRNPGPNYMWHIDSYDKLKPYGICINAAIDGFSRNIIWIKAYWTNSNPRIIAGYFMEEVEKLGGCPARVRCDMGTENVYLEQMQRFLRYDHVDEFARDCFIYGSSNHNQRIESWWSYLRKQHAQFWMNLFQQLKEENEFSGDLLDKSLVQFCFMNIIQNELNEVADLWNMHRIRRCRNAIAPNGRPLLMYNLPNQFGGTDHLQNVSRQQVRLCKDECTFRGRLPCAETVFEIACTIMAEHNISPPEDSKEAEEIYKILRRYIRLRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HFH7</accession>
    <name>A0A9Q1HFH7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Muscarinic acetylcholine receptor M2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_07253</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HFH7"/>
    <dbReference type="OrthoDB" id="10071887at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001591">
        <property type="term" value="F:dopamine neurotransmitter receptor activity, coupled via Gi/Go"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248">
        <property type="entry name" value="ADRENERGIC RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248:SF125">
        <property type="entry name" value="DOPAMINE D2-LIKE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="70"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="112"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="156"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="196"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="402"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="49"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="241"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8044489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="479" mass="53617" checksum="01FA8713AA538613" modified="2023-09-13" version="1">MTSALVTFNGSSETGTTEAPFSFTWELTPIFSPIESIPWYLLAFASIFLNGLILISFAKEKRLRTYINYYVINLAIADFCVSIYVMPVYAVVTMLGHYWPFFGYYGCKIFQGVSYSFVTVTILAVIAICSDRFYATVAPIHYFSHRSKGAAFRINVICWIVAFCIWIPFNTLWPIWTPSSLVVQTACGPDYGKTFLATMVAISVLFWFPILIITLMNATVYYEIAKSGALSVGKSFEDKSSSRRDTQVTSLSQDSMSHETSDGVTTSQAENAHKNRSFVPDNENPGMNEVTNNGTWIGAADVYLNQVKGENDETNEIQEGLLVVIRTNNKDITKNSSKNDIVVDGISRVPSKQIVVTSQSHDTTKTEGTTEKLNETAGLHKNSLSMNEQTRKKESVKESVKAFRILSVIVLSYFLSWVMFGVVVVYISLCWRGRQVKCTSGTVDLIAQYLSLCNSLFNPICYATVQPLLRETIAKIFRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HH08</accession>
    <name>A0A9Q1HH08_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Exportin-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_07465</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the exportin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HH08"/>
    <dbReference type="OrthoDB" id="27218at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005049">
        <property type="term" value="F:nuclear export signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006611">
        <property type="term" value="P:protein export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000056">
        <property type="term" value="P:ribosomal small subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:001255">
        <property type="entry name" value="Exportin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041123">
        <property type="entry name" value="CRM1_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041235">
        <property type="entry name" value="Exp1_repeat_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013598">
        <property type="entry name" value="Exportin-1/Importin-b-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045065">
        <property type="entry name" value="XPO1/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014877">
        <property type="entry name" value="XPO1_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040485">
        <property type="entry name" value="XPO1_repeat_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11223">
        <property type="entry name" value="EXPORTIN 1/5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11223:SF2">
        <property type="entry name" value="EXPORTIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08767">
        <property type="entry name" value="CRM1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18777">
        <property type="entry name" value="CRM1_repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18784">
        <property type="entry name" value="CRM1_repeat_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18787">
        <property type="entry name" value="CRM1_repeat_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08389">
        <property type="entry name" value="Xpo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01102">
        <property type="entry name" value="CRM1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="8">
        <location>
            <begin position="46"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00073514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8044671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1073" mass="122799" checksum="B1B42EE5BAE6D8B4" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HIP5</accession>
    <name>A0A9Q1HIP5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Metalloproteinase inhibitor 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_06101</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protease inhibitor I35 (TIMP) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HIP5"/>
    <dbReference type="OrthoDB" id="6041373at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008191">
        <property type="term" value="F:metalloendopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002020">
        <property type="term" value="F:protease binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051045">
        <property type="term" value="P:negative regulation of membrane protein ectodomain proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.370.10">
        <property type="entry name" value="Tissue inhibitor of metalloproteinase-1. Chain B, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001134">
        <property type="entry name" value="Netrin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001820">
        <property type="entry name" value="TIMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008993">
        <property type="entry name" value="TIMP-like_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027465">
        <property type="entry name" value="TIMP_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11844">
        <property type="entry name" value="METALLOPROTEASE INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11844:SF33">
        <property type="entry name" value="TISSUE INHIBITOR OF METALLOPROTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00965">
        <property type="entry name" value="TIMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00206">
        <property type="entry name" value="NTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50242">
        <property type="entry name" value="TIMP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50189">
        <property type="entry name" value="NTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0481">Metalloenzyme inhibitor</keyword>
    <keyword evidence="4" id="KW-0483">Metalloprotease inhibitor</keyword>
    <keyword evidence="5" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040166421" evidence="11">
        <location>
            <begin position="20"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="NTR" evidence="12">
        <location>
            <begin position="25"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared with metalloproteinase partner</note>
        </ligand>
    </feature>
    <feature type="site" description="Involved in metalloproteinase-binding" evidence="9">
        <location>
            <position position="39"/>
        </location>
    </feature>
    <feature type="site" description="Involved in metalloproteinase-binding" evidence="9">
        <location>
            <position position="68"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="25"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="27"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="38"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="165"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="189"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR601820-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR601820-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR601820-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8047175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="275" mass="31052" checksum="6E8CAC1EDCBEA9E7" modified="2023-09-13" version="1">MFGVVIIVSLTFLAGTSYGKNGQRCTCNMVHHVQEEFCNSDYALHVMIHAGVPSPLPGERDLGDINNKYMKYEVEVVESFKEDDFEKGQIINVYSPRHDICGKPGLMDEIRGGTSNEYLFVGDIGQIEDGDEFMVITNCNWVEPWEDIGESQRDGLRGMYTDCSCTIFPVSGPMDIGDTNADRFSYDNCFFNPVKAQVLKVKDCETLHGFCLRQSDRTCRWENTDAFQECFATRKSSLTTSAAAVTTKEGCKLFSGRKKRQCLLKFLKNRNNGRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HL17</accession>
    <name>A0A9Q1HL17_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Fucosyltransferase</fullName>
            <ecNumber evidence="11">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_01651</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Endomembrane system</location>
            <topology evidence="10">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Golgi apparatus</location>
            <location evidence="11">Golgi stack membrane</location>
            <topology evidence="11">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the glycosyltransferase 10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="11"/>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HL17"/>
    <dbReference type="OrthoDB" id="427096at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046920">
        <property type="term" value="F:alpha-(1-&gt;3)-fucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11660:FF:000002">
        <property type="entry name" value="Alpha-(1,3)-fucosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11660">
        <property type="entry name" value="Glycosyl transferase family 10, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055270">
        <property type="entry name" value="Glyco_tran_10_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001503">
        <property type="entry name" value="Glyco_trans_10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038577">
        <property type="entry name" value="GT10-like_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11929">
        <property type="entry name" value="ALPHA- 1,3 -FUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11929:SF145">
        <property type="entry name" value="ALPHA-(1,3)-FUCOSYLTRANSFERASE FUT-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00852">
        <property type="entry name" value="Glyco_transf_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Fucosyltransferase C-terminal" evidence="12">
        <location>
            <begin position="26"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00060399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8049078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="213" mass="24482" checksum="531562D638D89DF3" modified="2023-09-13" version="1">MLSYDARYFAPNYSKTALSRKEVVVSVAWISSRHCEGLGWDRTNFALDLKQYIPIDMYGKCGSRSLSRNSATATRVIQKCKFHLSLENSCCSEYLSEKFWNTLQKWKSVPIVVGGTKEEYERLAPPNSFIHADDFPSIEALAKYIQTVAESEKFFNECFKWHKEGSVHQKSFHSRHPVFKEGACKVVQKLHQLCNSPSEDSFDPYGHKWFGSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0Y954</accession>
    <name>A0A9Q0Y954_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_45236</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010002172">
        <property type="protein sequence ID" value="KAJ8017395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0Y954"/>
    <dbReference type="OrthoDB" id="2359399at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8017395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="225" mass="25752" checksum="F46DF61A4B3122FE" modified="2023-09-13" version="1">MINEDHVARTVEKITEYSKQKGNKSKFGCLSPPLFASIPVHRVVPDTLHLFKRISDQLINHLLVELKTFDNIKNCSASFDVKSCAHIYRFESFIQSLGIEWSFSVDTITKLVTARDFTGPELWKIVSNISLVDFTPCHSKLASLNKIWINFFNLINTLKYTLSTEEIITFDQSAKKWVELFCEVYLKKDVTPYMHVLSHHVAESLQLNGGILSQLSQEGLEKLND</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YBK6</accession>
    <name>A0A9Q0YBK6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_42497</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000081">
        <property type="protein sequence ID" value="KAJ8019120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YBK6"/>
    <dbReference type="OrthoDB" id="10069439at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053134">
        <property type="entry name" value="RNA-dir_DNA_polymerase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24559:SF454">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24559">
        <property type="entry name" value="TRANSPOSON TY3-I GAG-POL POLYPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8019120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="197" mass="22050" checksum="9E2FE583EB5AE744" modified="2023-09-13" version="1">MLAKVLVDPSREVVPLRIMNPTDRPITLYEGTVVGGCHPAEVLGRVEPATTRTSEAKKIPEPSQNKPDELEIHLHTLMNKSCTGLTQGEAKQVRKLLADNGDVFARFKGDLGRTDLVQHQINTGDARPIRQAPPRRLPIHKKEEAEKELEKKMLNQDVVEPSNSAWSSPVALVRKKDGSTRYCIDYRKLIDVTQKDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YBL7</accession>
    <name>A0A9Q0YBL7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_43392</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000293">
        <property type="protein sequence ID" value="KAJ8018549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YBL7"/>
    <dbReference type="OrthoDB" id="5376140at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33480">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33480:SF1">
        <property type="entry name" value="TYR RECOMBINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="182"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="198"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8018549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="324" mass="36650" checksum="414A57A614AEF59A" modified="2023-09-13" version="1">MMLPESGLTSVTSEIFSGLRSDESGVTLFIKTDTLTIQLAKKLSLKVGHDKEQYPYIRSKLREVGRMVVEYRNATGESSASLADLINPKKFIDVVHATRQTSGFNDEQHLYRTPSLALKIGHSLKKAAEILKGDALIKGDFELEKRSRAFDELCNMKEWKPPKHVWKSLDDIQISPDEEVPVDESIMDDSQKPSATTDDSDVTDVEDNSEHLGSEAISPAENSGVDNMSKAISPDENSDQFFIYITVVKKTRSVKKKPWMPQEKQIIATKFINSFVSSKLPGKKEILDIINQEPLLKSRKWTNVKDFIRKQLKRNDPMDFCQTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YCC8</accession>
    <name>A0A9Q0YCC8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_39705</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YCC8"/>
    <dbReference type="OrthoDB" id="10514024at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8020182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="120" mass="13953" checksum="E2BFF4B70045E62B" modified="2023-09-13" version="1">METVDHILYPGTYLVIDNVITFFFLSGNQYREPSLRAPSFDQLEEIATKLGLRFTDKDELKEYQAFIETFCEGFNAIDRLPEPTLPVKYPRFPGHRPKPEENPLNGWYVSARVVKLNRGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YEC5</accession>
    <name>A0A9Q0YEC5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_40971</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8021178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YEC5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34723">
        <property type="entry name" value="PROTEIN CBG17025"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34723:SF8">
        <property type="entry name" value="PROTEIN CBG17025"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8021178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="336" mass="37485" checksum="C977C803AE49757F" modified="2023-09-13" version="1">MKACYLLIVHIKCTVPSVHRTISTSCHQYIVPSVHRAISTSCHQYIVPSVHRAISASCHQCIVPSVHRAISASCHQCIVPSVHRAISTSYHQYIVPSVPRAISTSCHQYIVPSVHRAISTSCHQYIVPSVHRAISTSCHQYIVPSVHRAISTSYQQYIVPSVHRAISTSYHQYIVPSVHHAISASCHQYIVPSVHQYIVYIVPSVHRTISTSCHQCIMPSVHRAISTSCHQYIVPSVHRAISTSCHQYIVPSVHRAISASCHQCIVPSVHRAISTSCHQCIVPSVHRAISASCHQYIVPSVHRAISTSYHQYIVPSVHRAISTSYQQYTVHRTIRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YED6</accession>
    <name>A0A9Q0YED6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Protein gustavus</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_38320</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SPSB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YED6"/>
    <dbReference type="OrthoDB" id="5547302at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03718">
        <property type="entry name" value="SOCS_SSB1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12906">
        <property type="entry name" value="SPRY_SOCS1-2-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.750.20:FF:000001">
        <property type="entry name" value="Ankyrin repeat and SOCS box containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000007">
        <property type="entry name" value="SPRY domain-containing SOCS box protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.750.20">
        <property type="entry name" value="SOCS box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050672">
        <property type="entry name" value="FBXO45-Fsn/SPSB_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001496">
        <property type="entry name" value="SOCS_box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036036">
        <property type="entry name" value="SOCS_box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12245:SF11">
        <property type="entry name" value="PROTEIN GUSTAVUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12245">
        <property type="entry name" value="SPRY DOMAIN CONTAINING SOCS BOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07525">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00969">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158235">
        <property type="entry name" value="SOCS box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50225">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B30.2/SPRY" evidence="4">
        <location>
            <begin position="41"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="SOCS box" evidence="5">
        <location>
            <begin position="227"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8021193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="279" mass="31661" checksum="76E473C024BB407D" modified="2023-09-13" version="1">MSRSIVMGQKVSGGMKQIARESPSYKANHRDLAYNGDLQKPNRLDLLLDMPHADKEVQYKYAWNEEDRSLNIFVKEDDPMTFHRHPVAQSTDCIRSKGGFSRGLHVFEISWNPRQRGTHAVVGVATREAPLHCVGYQSLVGSNEESWGWDIGRKKAFHDSKHNAGLQYPLLENTDHFVVPEKFLMVLDMDEGTLSFIVHGKFLGIAFRGLKGKTLFPIVSAVWGHCEITLKYIGGLEPSPLPLQDLCRRVVRVSVGQENLENIYGLPLPNTMKRYILYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YFN3</accession>
    <name>A0A9Q0YFN3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Rho GTPase-activating protein 18</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_40816</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8021054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YFN3"/>
    <dbReference type="OrthoDB" id="27680at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030833">
        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051056">
        <property type="term" value="P:regulation of small GTPase mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057323">
        <property type="entry name" value="RHG40/28/18_ubiquitin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14963">
        <property type="entry name" value="RHO GTPASE ACTIVATING PROTEIN 18,19-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14963:SF1">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN CONUNDRUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25442">
        <property type="entry name" value="Ubiquitin_RHG40_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho-GAP" evidence="2">
        <location>
            <begin position="220"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8021054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="589" mass="66840" checksum="737FED9C112D0A08" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YHF2</accession>
    <name>A0A9Q0YHF2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_40189</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YHF2"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040431954" evidence="1">
        <location>
            <begin position="28"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8020564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="53" mass="5627" checksum="C1EEDB7A42F72B95" modified="2023-09-13" version="1">MAGSMIQIISVILLLVILAFALSYGAGEDVNISLCCTRERCAESTKNCLCCQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YHH3</accession>
    <name>A0A9Q0YHH3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_40245</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YHH3"/>
    <dbReference type="OrthoDB" id="10063284at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8020609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="193" mass="22576" checksum="5A00DF2BC9DEF528" modified="2023-09-13" version="1">MFFTSFTARFNQLKTVLDQEGLQVPKRLSDSQWSTHHYATKALLNGYYAIMGVLDELANDALQKGDVRNTAYTYQSMCKLEIGFYAIFWSTVLERFNSTRKSLQSESFDLNTTVELLQTLLAFVESLRNSDRFHAFIEKGIRRSGTDQFEHHTRRRKRSVPLDPLDHGRTRRSFEMPNCMCMCCNIFNIRDFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YIA6</accession>
    <name>A0A9Q0YIA6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">E3 ubiquitin-protein ligase TRIM56</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_40839</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8021074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YIA6"/>
    <dbReference type="OrthoDB" id="6160893at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19757">
        <property type="entry name" value="Bbox1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047153">
        <property type="entry name" value="TRIM45/56/19-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25462">
        <property type="entry name" value="BONUS, ISOFORM C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25462:SF296">
        <property type="entry name" value="MEIOTIC P26, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="16"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="7">
        <location>
            <begin position="94"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="7">
        <location>
            <begin position="155"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="247"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8021074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="380" mass="43861" checksum="5401C27D6E46066A" modified="2023-09-13" version="1">MAQSVNRSFREDLVHCVICSEDYNEPTLLPCLHSFCLKCLKRWASNSQAQPLSCPTCGCTVDLPDAGVGSLPQNVFLVSLAERVEEAGRLSKQRKDCSCDICRTNIGAMFCLDCKMHICSDCLGTHERLSRSRDHNMIPSHSLSDQTYLKKIMLTQAPYCTKHRQEKISFFCTQCEQLVCHVCALVCHIGHNLQEAEIKVKSVRANMQTILKESEIEISSILDKSKETERSAEEIKSKLSALLCDVDARYEQIVSKLQSDRKQLRQELFAIERRKSEYLQCINADISMWFKKMENNLETIRRILEEKNPWEMVQMERNIIWSYKKLQSDKSRVEMFPLPGKIAFTFIASDLGRVPHEFREKKPRNSINENFLGSLYKTWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YIU8</accession>
    <name>A0A9Q0YIU8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">RUN and FYVE domain-containing protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_37174</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YIU8"/>
    <dbReference type="OrthoDB" id="5872154at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15727">
        <property type="entry name" value="FYVE_ZF21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052113">
        <property type="entry name" value="FYVE-type_Zinc_Finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39490">
        <property type="entry name" value="ARRESTIN DOMAIN-CONTAINING PROTEIN D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39490:SF9">
        <property type="entry name" value="FYVE-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FYVE-type" evidence="5">
        <location>
            <begin position="40"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8022316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="473" mass="51813" checksum="4BB5496739573AD2" modified="2023-09-13" version="1">MIPGAVKIEGVGVLPAQTEGRADVYSDNNYILAKSKWMPDDEATHCPLCQQKFSQLRRKHHCRQCGQVFCSKCCKTNVPLPQLGLENPERVCDGCLPVVELVTKSKSPQGSFQSESIQGLLEQIHDSTGLRKVVQLGGVQAIISLSRSSNSEVRKKCAEALHLLAMQEPLHNMLIDAGAIKALCGLLMSASETDEQLLSYLIGALMIFAKAPHLQMRVVQDGALPPVLALCGMNVSEAIALIAVRTLNLLMENIGSHTALIESDRNALPRLLKLTVSMDEQVKLFYISVGTTTGDIRQMQEVSLKTLSRLSMGTDYHRHRIVQEDFSAGKCLMKSLQCHPKNLQVLCNAACLVANLATSEQDQSSLTECLDALCSLLTKFKEQVDLLVHVIRGLANFASFHQNVYRLSTNEAVQQHSLRLILHVLHRSSEQAITALMRDGGADLLRSIAKTPGMVDAMQANLRQYVSALSAPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-04-02" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YKV0</accession>
    <name>A0A9Q0YKV0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Dynein heavy chain domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_34318</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the dynein heavy chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="5986589at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008569">
        <property type="term" value="F:minus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.1220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.720">
        <property type="entry name" value="Region D6 of dynein motor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035706">
        <property type="entry name" value="AAA_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041658">
        <property type="entry name" value="AAA_lid_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042219">
        <property type="entry name" value="AAA_lid_11_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041228">
        <property type="entry name" value="Dynein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043160">
        <property type="entry name" value="Dynein_C_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024743">
        <property type="entry name" value="Dynein_HC_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024317">
        <property type="entry name" value="Dynein_heavy_chain_D4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004273">
        <property type="entry name" value="Dynein_heavy_D6_P-loop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45703">
        <property type="entry name" value="DYNEIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45703:SF36">
        <property type="entry name" value="DYNEIN HEAVY CHAIN, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12780">
        <property type="entry name" value="AAA_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12781">
        <property type="entry name" value="AAA_9"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18198">
        <property type="entry name" value="AAA_lid_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18199">
        <property type="entry name" value="Dynein_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03028">
        <property type="entry name" value="Dynein_heavy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12777">
        <property type="entry name" value="MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynein heavy chain AAA module D4" evidence="6">
        <location>
            <begin position="189"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain coiled coil stalk" evidence="5">
        <location>
            <begin position="514"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain ATP-binding dynein motor region" evidence="7">
        <location>
            <begin position="855"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain ATP-binding dynein motor region" evidence="7">
        <location>
            <begin position="1027"/>
            <end position="1197"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain region D6 P-loop" evidence="4">
        <location>
            <begin position="1484"/>
            <end position="1603"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain AAA lid" evidence="8">
        <location>
            <begin position="1644"/>
            <end position="1731"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain C-terminal" evidence="9">
        <location>
            <begin position="1815"/>
            <end position="2104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="917"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="480"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="736"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="918"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="931"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="950"/>
            <end position="959"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF12777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF18199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8024415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="2113" mass="238491" checksum="80AE78B8033145FD" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YM06</accession>
    <name>A0A9Q0YM06_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Melanopsin-A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_38499</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YM06"/>
    <dbReference type="OrthoDB" id="5975505at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00637">
        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24228">
        <property type="entry name" value="B2 BRADYKININ RECEPTOR/ANGIOTENSIN II RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24228:SF59">
        <property type="entry name" value="NEUROPEPTIDE RECEPTOR 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="20"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="60"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="93"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="141"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="195"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="10">
        <location>
            <begin position="39"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8021331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="236" mass="26892" checksum="EF6FC86C22A11FF9" modified="2023-09-13" version="1">MNVSEQPEINSEIQLNGISWFHYANASLFIVAIVLGFLGNSLVVLAFILSKRIRTTTNIFIVNLAVTDILTTAPMFCVVWLSVSNEEAIIHSLNLWCSLLLLLGRGLGGCSILTLVLIAVSRWILITKRIETYRYIYRRKTVFIILLLTWFYSIVLSCLPGFLNDIHYNFNGTFRICQITEKSYQFSFLNISSSIVPYYTIAPLIVIYCYGSIFKHVKTNNRRIDGKRTVVRTISG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YPM6</accession>
    <name>A0A9Q0YPM6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Cubilin-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_34022</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8026229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YPM6"/>
    <dbReference type="OrthoDB" id="5804959at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24251">
        <property type="entry name" value="OVOCHYMASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CUB" evidence="4">
        <location>
            <begin position="74"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8026229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="190" mass="21232" checksum="F3018340DD676678" modified="2023-09-13" version="1">MTTTTGPTTRLTTEQTTVYEGLPPSSGNYRSPRRCGTSAIPVVTTNGFIYIHVYTDSSVQSGGMLGRLVFSKMCGKTYSKNGQEISSPFYPDSLPEGITEYVCEFRITAPTGSTVRIEFSFLHMEAHDECIYDRIEFYNEADINGEPLQIVCGETVPTNPVYLNTDSVVVKVISDYAYTSYGFVFTYWFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YR25</accession>
    <name>A0A9Q0YR25_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Mitoferrin-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HOLleu_33959</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Nanhai2018</strain>
            <tissue evidence="15">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8026186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YR25"/>
    <dbReference type="OrthoDB" id="43906at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015093">
        <property type="term" value="F:ferrous iron transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048250">
        <property type="term" value="P:iron import into the mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000029">
        <property type="entry name" value="Solute carrier family 25 member 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45758">
        <property type="entry name" value="MITOFERRIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45758:SF20">
        <property type="entry name" value="MITOFERRIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0410">Iron transport</keyword>
    <keyword evidence="12 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 13 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 14" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="13">
        <location>
            <begin position="65"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="13">
        <location>
            <begin position="161"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="13">
        <location>
            <begin position="252"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ8026186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="352" mass="38806" checksum="6D5BFD5D228B897E" modified="2023-09-13" version="1">MPTETLAFTRMAEISSPKIRGAISVSPSIPRIPDHGFRVRISDMDNPESQHVVDEHDYESLPETSTFTTHMAAGGIAGIMEHCVMYPVDCVKTRMQALAPAPGTPHYKNVGNALKTIVRQEGLTRTFRGLPLVATGAGPSHALYFACYEKLKKVLSSQPGKNPVANAVAGGVATIVHDAAMNPVDVIKQRLQMYSSPFKSTVQCMKHVYRTEGFGAFYRSYTTQLTMNVPFQILHFVTYEFGQEFLNPSRHYNPVTHMVAGGFAGATAAALTTPLDVCKTLLNTQESYIVSEKNGIAISGMFNALRTVYDKQGIRGYFKGLQARVIFQIPATALSWSVYEFFKYSISKQRNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YR65</accession>
    <name>A0A9Q0YR65_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">RNA-directed DNA polymerase from mobile element jockey</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_36314</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YR65"/>
    <dbReference type="OrthoDB" id="411173at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47510:SF3">
        <property type="entry name" value="ENDO_EXONUCLEASE_PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47510">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8023776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="218" mass="25171" checksum="4C3B62558C8E36E8" modified="2023-09-13" version="1">MINEKKRIFGTGNKDKLKETQRRLNRAVRKEKLRYKKKVEKYFTDNNMAQVWRGLRLMSGYTRGDNKSWQLPEVSTSYADEVNTFYNQFDSLDFSNGIYYLRTQLTYNPEPFLVISEDKVRYQFAKINPSKAAGPDMLSPRVLRTCSGKLAGIFTHIFNLSFSTLAIPEIWKQSCIIPVPKSSTVSCMDDLRPVALTSVVMKICERFVLTSLRKVIAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YRF1</accession>
    <name>A0A9Q0YRF1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Nucleosome assembly protein 1-like 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_36504</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the nucleosome assembly protein (NAP) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YRF1"/>
    <dbReference type="OrthoDB" id="27325at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1500:FF:000001">
        <property type="entry name" value="Nucleosome assembly protein 1-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1120.90:FF:000001">
        <property type="entry name" value="Nucleosome assembly protein 1-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1500">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1120.90">
        <property type="entry name" value="Nucleosome assembly protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037231">
        <property type="entry name" value="NAP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002164">
        <property type="entry name" value="NAP_family"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11875">
        <property type="entry name" value="TESTIS-SPECIFIC Y-ENCODED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00956">
        <property type="entry name" value="NAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143113">
        <property type="entry name" value="NAP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="138"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="369"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="139"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="369"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8023926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="485" mass="55173" checksum="DDE433B657190038" modified="2023-09-13" version="1">MADSQNTEVAATATPDTVVDLEAGAQGDANIEEVEDIDMKEDKESAASLDLKKQVLQNPKVLAALQDRLDSLVGQRSGYIDLLPAAVKRRIKALKNYQVKQVQLEAKFYEEVHQLECKYAKLYEECYEKRKNVVTGEVEPTDSDCEWESSEDEDDEEEAGGDGKEVKALAQEVKNKVTLVDNENTKGIPEFWLTIFKNVDILAAMIQDHDEPILKHLEDIRVIFHEGAEMGFTLEFHFTPNEFFNNRVLTKKYEMKSEPDEADPFSFEGPEIVGAKGCKIDWKKGKDVTVKIIKKKQKHKGRGTTRQVTKTITNDSFFNFFKPPEVPEDEAEMDEETELLLSSDFEIGHMIRERIVPRAVLYFTGEAIEDDEYEEEGEEEEGEGEDEEDEENDPDYEPSKEANWLIEDPIPERNHLSVSHSDLLPSKMFKTSTTIYLWMDAIELDSFIALLRFGLTPIVSLLRDVIRAAIVGVRLKSGFELCILA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YRW7</accession>
    <name>A0A9Q0YRW7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_37048</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8024331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YRW7"/>
    <dbReference type="OrthoDB" id="6141539at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8024331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="89" mass="10149" checksum="C06534BBC6314C2F" modified="2023-09-13" version="1">MVEPSGKPIIMYTSPELYNTDNKLVLVDALEVEVCIQQCVFKDGQTCSDATVFRLCCDLMVEHDLDVPHNPQEAIILYNTLRDAIYREL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YSG5</accession>
    <name>A0A9Q0YSG5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Aminopeptidase NAALADL1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">N-acetylated-alpha-linked acidic dipeptidase-like protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">HOLleu_31708</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">Nanhai2018</strain>
            <tissue evidence="19">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Aminopeptidase with broad substrate specificity. Has lower activity with substrates that have Asp or Glu in the P2' position, or Pro in the P3' position. Lacks activity with substrates that have both Pro in the P3' position and Asp or Glu in the P2' position. Lacks carboxypeptidase activity. Lacks dipeptidyl-peptidase IV type activity.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Apical cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M28 family. M28B subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YSG5"/>
    <dbReference type="OrthoDB" id="5841748at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004180">
        <property type="term" value="F:carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000101">
        <property type="entry name" value="N-acetylated alpha-linked acidic dipeptidase like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.930.40:FF:000001">
        <property type="entry name" value="N-acetylated-alpha-linked acidic dipeptidase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.40">
        <property type="entry name" value="Transferrin receptor-like, dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039373">
        <property type="entry name" value="Peptidase_M28B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007365">
        <property type="entry name" value="TFR-like_dimer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036757">
        <property type="entry name" value="TFR-like_dimer_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10404:SF77">
        <property type="entry name" value="GLUTAMATE CARBOXYPEPTIDASE 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10404">
        <property type="entry name" value="N-ACETYLATED-ALPHA-LINKED ACIDIC DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04253">
        <property type="entry name" value="TFR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47672">
        <property type="entry name" value="Transferrin receptor-like dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M28" evidence="18">
        <location>
            <begin position="347"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="domain" description="Transferrin receptor-like dimerisation" evidence="17">
        <location>
            <begin position="616"/>
            <end position="739"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00059290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00068168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00081462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF04253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAJ8026775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="744" mass="84321" checksum="01007C0B60312418" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YSS4</accession>
    <name>A0A9Q0YSS4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_35085</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8025006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YSS4"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8025006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="134" mass="15583" checksum="E2C354C72E902219" modified="2023-09-13" version="1">MVQSNADGSKLEVSHKTSVYFVSNLHLTGAEEHLLSKGIKFCPTPLHTDKLLLQKDLDTFKRRLSLFEFVNADCQTGDEEPSRLEVLLFKNKSMWVPREERDKFLECFLKAVRKDVEDFQPLNLLRIICLEEIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YTF8</accession>
    <name>A0A9Q0YTF8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Blood vessel epicardial substance</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_31801</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the popeye family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YTF8"/>
    <dbReference type="OrthoDB" id="425611at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042383">
        <property type="term" value="C:sarcolemma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030552">
        <property type="term" value="F:cAMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051146">
        <property type="term" value="P:striated muscle cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006916">
        <property type="entry name" value="POPDC1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055272">
        <property type="entry name" value="POPDC1-3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12101">
        <property type="entry name" value="POPEYE DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12101:SF30">
        <property type="entry name" value="POPEYE DOMAIN-CONTAINING PROTEIN 3-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04831">
        <property type="entry name" value="POPDC1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="73"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="POPDC1-3" evidence="7">
        <location>
            <begin position="21"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8026849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="269" mass="31017" checksum="C2BD76CB675C58AC" modified="2023-09-13" version="1">MDLKEDVHYPLSTCPSWVTNNSIVFQLSCGLLTLGFLIPHRGVLTSFVAHLLIALGHIIFSLWGVVFVCSPDILGWNLLFALLHFVHIGYFAWRLRPVFLSRELKDLYQVVFKPFAVSKREFRNLSQMGKIYTLKNGECYAEEGKTSTGNLSIILYGKVKVYCGGEYLHHILDKQFLDSPEWDAYDSNNQAEKFQVTLKATSYCKYLMWERETLQEYLDSDSFMKQIFINLIGHDVARKLTLLTERQWGHVGIKPDIRLSALEADTEHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YUE7</accession>
    <name>A0A9Q0YUE7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tyr recombinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_31935</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YUE7"/>
    <dbReference type="OrthoDB" id="6755924at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="entry name" value="Intergrase catalytic core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35617:SF3">
        <property type="entry name" value="CORE-BINDING (CB) DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35617">
        <property type="entry name" value="PHAGE_INTEGRASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyr recombinase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8026956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="286" mass="33221" checksum="745DD858442DDB82" modified="2023-09-13" version="1">MILALISAQRVQALSFLHMDHMKMGTNLVTFQFHDLLKQNKPENLSPTLLIRGYPPDRRLCAVKYLRHYVEETSTIRGAERYLLTSYKKPFKRVTAQTVSRWLKEVMKALGINTDVYKAHSTRAAATSAASTSAVSIQDIMAQARRSNERTFRTYYNKPVTPTKDLSRSSALYIMYIHGRPQKKIQGGAKFFSRANFFHGKYFTPRKEFRCFFFHTVLTKAWPRICQGGGAKPIDRLSALRATMGWRGAQENFGPKCFPDRWKWHFQGLFTAYKKGKHKQYRKYKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BB03</accession>
    <name>A0A9Q1BB03_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_43555</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000358">
        <property type="protein sequence ID" value="KAJ8018454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="5376140at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33480">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33480:SF1">
        <property type="entry name" value="TYR RECOMBINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="680"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="710"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="776"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="788"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="832"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="867"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="894"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="922"/>
            <end position="939"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8018454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1408" mass="158460" checksum="0A583C85BC78B5C8" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BD28</accession>
    <name>A0A9Q1BD28_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_39800</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BD28"/>
    <dbReference type="OrthoDB" id="5988155at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46670">
        <property type="entry name" value="ENDO/EXONUCLEASE/PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46670:SF3">
        <property type="entry name" value="ENDONUCLEASE_EXONUCLEASE_PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8020252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="119" mass="13156" checksum="D446122D32B5B3B1" modified="2023-09-13" version="1">MDLTLSSLSPSLRLLLLYKPSPSKKYKLTFSMLLHDFGCLTESLISDPCHFLFAGDFNIHVDASDNHDAVSFRNLLDTSDFHQHVTGSTHKAGPTLDLLISNNCDDLISSVSTLPRSSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BEI6</accession>
    <name>A0A9Q1BEI6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_37109</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BEI6"/>
    <dbReference type="OrthoDB" id="6142091at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8022264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="101" mass="12303" checksum="5B1F42C2812F80DD" modified="2023-09-13" version="1">MRKDEIKRYVLEYLVDEGVLPSTVLETAITVPTDNTFELKRLEIEMNKEIRLKEMEREMQKEKEEREMQMQKEKEEREMQMQKGKRGKRRKRKKGGKGKRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BEJ9</accession>
    <name>A0A9Q1BEJ9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_37503</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BEJ9"/>
    <dbReference type="OrthoDB" id="6122461at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366">
        <property type="entry name" value="IG(IMMUNOGLOBULIN) AND LRR(LEUCINE RICH REPEAT) DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366:SF96">
        <property type="entry name" value="LEUCINE RICH REPEAT CONTAINING 53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="521"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="555"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="578"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="720"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1039"/>
            <end position="1062"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1074"/>
            <end position="1091"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1097"/>
            <end position="1116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="98"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="604"/>
            <end position="633"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8022563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1153" mass="128928" checksum="E6711EA3A1D6A9AA" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BFZ4</accession>
    <name>A0A9Q1BFZ4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Betaine--homocysteine S-methyltransferase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_35267</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="4">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8025145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BFZ4"/>
    <dbReference type="OrthoDB" id="261426at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.330">
        <property type="entry name" value="Homocysteine-binding-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017226">
        <property type="entry name" value="BHMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003726">
        <property type="entry name" value="HCY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036589">
        <property type="entry name" value="HCY_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11103">
        <property type="entry name" value="SLR1189 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11103:SF18">
        <property type="entry name" value="SLR1189 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02574">
        <property type="entry name" value="S-methyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037505">
        <property type="entry name" value="Betaine_HMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82282">
        <property type="entry name" value="Homocysteine S-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50970">
        <property type="entry name" value="HCY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Hcy-binding" evidence="6">
        <location>
            <begin position="27"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4 5">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 5">
        <location>
            <position position="367"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 5">
        <location>
            <position position="368"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00034478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR037505-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8025145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="423" mass="47299" checksum="F0B398E460E92968" modified="2023-09-13" version="1">MAPRLQKIHFPDPQRHTMKGYFTQSIGLLERLNKGEKVVVAEGYLYNFEKRCYLKAGPYVPTVVLEHPDLVKQLHDEFVRAGSDVVLAFTYYGNRQKMNLIDRAHDLEELNRSALRITRKCADETGTLMAGNICNTNAYNPADPNNRENLKGMFKQLPQKHSNKSSKLFTNSQGGLISPSCSLLYPIGSSNGEWEGAGRSRFNGPLEEGADFIVAETFTVFDEAMIALESIKECSKGLPCVVTLAIMHNPAEDGEERTVDSVKVTEAMKRLEASGADVIGLNCMRGPETTIGLMEKIKKSGVKAHLDAVPVPYRTNEKAPIFMLLKDPKTGKRAFPYNMDCFMSTRDDIFEFGRRCDEIGISYIGVCCGNSPHYTRTLCESIGRTPPASKYSPDMNFHCVFGDEDHTDKYRKEQGEKLQGHKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BI17</accession>
    <name>A0A9Q1BI17_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_32048</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BI17"/>
    <dbReference type="OrthoDB" id="10060767at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008028">
        <property type="term" value="F:monocarboxylic acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF303">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="112"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="141"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8027033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="247" mass="27475" checksum="408C0B52EF4B8EDF" modified="2023-09-13" version="1">MILPVVFDRFQCLFGTQGAFLLFGGLNFNILVAGVSLRTSADHQLLHNDDHHQEEGWSIRLFLRYYWLVLVRNPDIWFLCSTSFLHYFNMIGWALFLVSLGEELGYSRSRAVLLSSCGGFGALLGRVWTLILFFHNVHNPFQFFGIPALMQCASFIGIVFTRNMFYLSAIMTLVSGISFGTTGAATVGVCALKTSNEDFTTALTILFMICAFGNEIGGGVCGKCLNDIHIAVTLLPVSSEKNRKEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BIZ6</accession>
    <name>A0A9Q1BIZ6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glutathione S-transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_32643</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BIZ6"/>
    <dbReference type="OrthoDB" id="414243at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF1">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14497">
        <property type="entry name" value="GST_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST C-terminal" evidence="1">
        <location>
            <begin position="89"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8027490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="230" mass="26079" checksum="9C84A7134B94AF33" modified="2023-09-13" version="1">MSGKALEVYSRIPSDEVKDYKKLKVALLQRYNLTEDGFRDKLRNSRIKPARDCNSRLRQSNERSNANPNRGAAVAVINEINVISDHTQTETERVQVLFAGIQAFDNICGPHWTFQPEEKRPDIIKNAINRLKNQKTDTFQPLKRSAFKENGTGFLVGNSPTIADCALFHNVSFFDEMPEYGDLLDGFPHCKAFLTKFSEIPGVKKYINGPRRFPVPDDEYTVAVKAALNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BJY2</accession>
    <name>A0A9Q1BJY2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cytoplasmic FMR1-interacting protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_30110</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the CYFIP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8027992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BJY2"/>
    <dbReference type="OrthoDB" id="10265867at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030833">
        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009828">
        <property type="entry name" value="CYRIA/CYRIB_Rac1-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008081">
        <property type="entry name" value="Cytoplasmic_FMR1-int"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12195">
        <property type="entry name" value="CYTOPLASMIC FMR1-INTERACTING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07159">
        <property type="entry name" value="CYRIA-B_Rac1-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05994">
        <property type="entry name" value="FragX_IP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF008153">
        <property type="entry name" value="FMR1_interacting"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01698">
        <property type="entry name" value="CYTOFMRPINTP"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CYRIA/CYRIB Rac1 binding" evidence="3">
        <location>
            <begin position="62"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00025790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR008153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8027992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1248" mass="144602" checksum="CCB2BEEE921E3487" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BK64</accession>
    <name>A0A9Q1BK64_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Histamine N-methyltransferase A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_30304</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BK64"/>
    <dbReference type="OrthoDB" id="5984880at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8028150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="317" mass="36383" checksum="85CD4FC1A02B692C" modified="2023-09-13" version="1">MLTTYCHTYSLSTVVANRPTAKAMDISSLPRIDVSSTVYSERYAVYIAYSNKVKMMEWISDRFDEEITYHLPNNGDLKLSILGIGSGDGKFEVKMIMKLKKKFRLIDCTVVEPSAERMASYRKLVKDEKNALFGVTFDWRQETFQEFCKANPDRSKKFHFVSLIHCAYYIHKRDLDYYMDVITRWTEGKILIMQDADVSIFGLLDKKFPGVYNGLRPLILGDTIMDSFKTLGMSFETSSLPCELDITACFDNDSRDGKLLLDFLLQVVGLVDDGPPEALKMVKEFLKSEGFSYTRNGRNWVENPTQVIIITPTSRKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BL89</accession>
    <name>A0A9Q1BL89_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_30821</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BL89"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8028550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="96" mass="11329" checksum="6819514E731B1031" modified="2023-09-13" version="1">MDRAYCTNVLCVNCRHRTYTSRLTNMAACYSLAFKSLDLLKWLDIDKLCEKLVIFSIECKLRSNSSIRMEDCDIVLPFILIFYLKCCRFIVKLGKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BMJ3</accession>
    <name>A0A9Q1BMJ3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">LRAT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_28758</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BMJ3"/>
    <dbReference type="OrthoDB" id="6102890at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1720.10">
        <property type="entry name" value="endopeptidase domain like (from Nostoc punctiforme)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007053">
        <property type="entry name" value="LRAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04970">
        <property type="entry name" value="LRAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LRAT" evidence="1">
        <location>
            <begin position="89"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8029380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="572" mass="64859" checksum="21D18CC964B26240" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BNJ2</accession>
    <name>A0A9Q1BNJ2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tyr recombinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_29264</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BNJ2"/>
    <dbReference type="OrthoDB" id="10064229at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="entry name" value="Intergrase catalytic core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010998">
        <property type="entry name" value="Integrase_recombinase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35617:SF3">
        <property type="entry name" value="CORE-BINDING (CB) DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35617">
        <property type="entry name" value="PHAGE_INTEGRASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8029781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="321" mass="36513" checksum="D3994F9777605385" modified="2023-09-13" version="1">MFHFRESLHIRGVSPRAAKIILAAWRPSTRKQYSVYLSRWLLFCGKRSLDPLQATVNSVLDFLVDLYDSGLGYSGINIARSMLSSFLVVPRDTGKEILIKKFLKGVFELRTPQPRYHSIWDVSVVLDYIRTLGPNNELTLQFLTYKTVILTALIPAQRVQTLRYLDLSHLRKTDESFCFFIVDKVKQTRPGHVGLEVIFSKYHNDERICVYSALEAYIEKTRTLRHKSNLFISYRKPNGPVGTETISRWIKAVLAPSGVNTNIFKAHSTRAASTSAASTKCVPVQKILNTAGWSNAKTFAMFYKKKVESHDNAFASSLLQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BQQ9</accession>
    <name>A0A9Q1BQQ9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="16">DNA repair protein complementing XP-G cells</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HOLleu_28018</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Nanhai2018</strain>
            <tissue evidence="16">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the XPG/RAD2 endonuclease family. GEN subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the XPG/RAD2 endonuclease family. XPG subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8031323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2959108at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017108">
        <property type="term" value="F:5'-flap endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008821">
        <property type="term" value="F:crossover junction DNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000400">
        <property type="term" value="F:four-way junction DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09904">
        <property type="entry name" value="H3TH_XPG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09868">
        <property type="entry name" value="PIN_XPG_RAD2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000030">
        <property type="entry name" value="Flap endonuclease GEN-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036279">
        <property type="entry name" value="5-3_exonuclease_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008918">
        <property type="entry name" value="HhH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006086">
        <property type="entry name" value="XPG-I_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006084">
        <property type="entry name" value="XPG/Rad2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001044">
        <property type="entry name" value="XPG/Rad2_eukaryotes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019974">
        <property type="entry name" value="XPG_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006085">
        <property type="entry name" value="XPG_DNA_repair_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16171">
        <property type="entry name" value="DNA REPAIR PROTEIN COMPLEMENTING XP-G CELLS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16171:SF7">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00867">
        <property type="entry name" value="XPG_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00752">
        <property type="entry name" value="XPG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00853">
        <property type="entry name" value="XPGRADSUPER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00066">
        <property type="entry name" value="XRODRMPGMNTG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00279">
        <property type="entry name" value="HhH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00484">
        <property type="entry name" value="XPGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00485">
        <property type="entry name" value="XPGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47807">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00841">
        <property type="entry name" value="XPG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="XPG N-terminal" evidence="15">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="XPG-I" evidence="14">
        <location>
            <begin position="805"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="320"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="364"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="492"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1054"/>
            <end position="1136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1200"/>
            <end position="1263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1305"/>
            <end position="1698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="321"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="341"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="369"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="387"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="492"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="529"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="582"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="603"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="622"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="638"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="673"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="1063"/>
            <end position="1074"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1075"/>
            <end position="1087"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="1108"/>
            <end position="1129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="1218"/>
            <end position="1230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1305"/>
            <end position="1318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1352"/>
            <end position="1364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1371"/>
            <end position="1382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1400"/>
            <end position="1410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="1418"/>
            <end position="1435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1479"/>
            <end position="1512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="1514"/>
            <end position="1525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1526"/>
            <end position="1536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1568"/>
            <end position="1578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="1616"/>
            <end position="1635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="1652"/>
            <end position="1662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="1663"/>
            <end position="1677"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ8031323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1698" mass="194025" checksum="7EC6A994D97C8A0D" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BQX4</accession>
    <name>A0A9Q1BQX4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">NADPH-dependent aldehyde reductase ARI1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_27823</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the NAD(P)-dependent epimerase/dehydratase family. Dihydroflavonol-4-reductase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8031177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BQX4"/>
    <dbReference type="OrthoDB" id="2735536at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050425">
        <property type="entry name" value="NAD(P)_dehydrat-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366">
        <property type="entry name" value="NAD DEPENDENT EPIMERASE/DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366:SF564">
        <property type="entry name" value="STEROL-4-ALPHA-CARBOXYLATE 3-DEHYDROGENASE, DECARBOXYLATING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="3">
        <location>
            <begin position="10"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8031177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="272" mass="30062" checksum="EE9079696CCF1AFD" modified="2023-09-13" version="1">MHTASPFPMTTPSNEDEVIKPAVDGTLNVLKACTGKVKRVVLTSSCAAVGDQHSPDRKLTEDDWTDTCNPELNTYVKSKRLAEQAAWDYVKGLQDDEKFELAVINPCLVVGPVLFKQQGTSIDLMSQLMCGKLPALPNFSMATVDVRDVAEAHLVGMTLPEAAGHRHITASDLATLMEMSEIFKEEFAPQGYKPTTRAMPKFVVSIMAVFSAQGRAFKSRWGRETPYDNNRLKSVLKIEPRPLKESLVDMGYSLIETGIVPKTKEYKGRPAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BRI8</accession>
    <name>A0A9Q1BRI8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_28067</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8031359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BRI8"/>
    <dbReference type="OrthoDB" id="5965607at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="239"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="90"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="341"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="453"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="466"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8031359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="492" mass="54361" checksum="5E8A9B2935259704" modified="2023-09-13" version="1">MASSINKWLGKSTGLQAVLDKQKSIQECMVKLNPCRTNVSAALLKWSMTESNLSLFDAFSSWADLDHALVQLSQAVIDQNFAFRQTFKNIQGEQKRLADLQKDTDNASKKVNALVSKLDSARKAKKVDAVRVENLEAELMLAKGKEKEMISILESKAKDFEYYKGKCVKDILNTLSEAYVQCLEKSLQIWNAKLQIAKAMADEPVYHGENLETESHLKVSNLFSQLSLELPIREDAPDMKHRTLPAQPPGSSSPALRHIASVPAVGNKRSPRVPDRRSEGDSYQWEVPKESSEDEEDYAFPDQIPDDHGEVYLVPGAAEDSKHPTPPGTPPQTSSDEMGGTDSTESSAQHLGADKTSSLNRGIKVLPAGGTMKGTNPLLKDSSQEQNTDVWKRTGRKTPPPSPPPLKNDGNNPQMYTNHGVDSVKRFPPTTKVPDRPITKKPAVRPKPPTLTKKLSSQNTSKESLQDIDEDEHEYSDVPIEPDTDSNTSPKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BTB7</accession>
    <name>A0A9Q1BTB7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_25760</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BTB7"/>
    <dbReference type="OrthoDB" id="97058at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47481">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47481:SF22">
        <property type="entry name" value="RETROTRANSPOSON GAG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="129"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="144"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8032274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="179" mass="20080" checksum="460987EA45E42FC6" modified="2023-09-13" version="1">MGPVQQLEGVEIGFFFDGVRTTERHREKSVTDYVLRAEKAATALNNAGENVSDSLVIAMILKGLPVEYKAFVVVVTQNEKEITFQDFKVMLRSYEETEKAQTSQQSDSIFKTMSDKQRQGANNPHQGVKCYSCGNFGHYARDCDTKGKTSSTGPTKRLGTLVGHKVPSCRPQRWPEKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BU54</accession>
    <name>A0A9Q1BU54_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">LRAT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_26290</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BU54"/>
    <dbReference type="OrthoDB" id="6102890at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1720.10">
        <property type="entry name" value="endopeptidase domain like (from Nostoc punctiforme)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8032694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="460" mass="52102" checksum="8ABE1698AEA6D5C5" modified="2023-09-13" version="1">MAGMMLRITTLQGSCQCGNSNPCLFRAYDDSEDHTMLTGVACKIRKCGTEYLTEALYPGVNVNNSFIIANGVKLYRKAPTLLELDPDFQTRLSRQRIANVTDVTPGDHVCFDRWYLLLHHAIVTEVRAQPPTLVVEGWDTTNWETGFYYVGKMKRAKRQISENELQYMYKFKYSEEVAMQNPPQLVLARCRALSHSDNDRACFPWLWYLGKLDDGYGFFNENCEHHAMYAKIGCRSSNQIKWLWLKIQHLFFKLSADFLKSAARNLGSVIAAEAVENLAQKCLRFAHSVLFEGIGLLGVVGVESYAFHKAYEALERRRLNNDIDNDRYQIELGKRVFEFFLTITLSGGGGILAAFFTSSALLEILLCVLLFVAAKAGMRFLEHFIGEDIGLWQWMADRLATVPIKLVNFANSIVDVGRSIANSVVDVGRSIVNTVETVATEICTEIRSLIDFFLSYFSQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BUL6</accession>
    <name>A0A9Q1BUL6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">DNA-binding protein inhibitor ID-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_23062</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8032963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BUL6"/>
    <dbReference type="OrthoDB" id="10047910at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032922">
        <property type="term" value="P:circadian regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19684">
        <property type="entry name" value="bHLH_dnHLH_ID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026052">
        <property type="entry name" value="DNA-bd_prot-inh"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11723">
        <property type="entry name" value="DNA-BINDING PROTEIN INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11723:SF17">
        <property type="entry name" value="PROTEIN EXTRA-MACROCHAETAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="6">
        <location>
            <begin position="25"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8032963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="166" mass="18574" checksum="D0EFDD26A0BC4423" modified="2023-09-13" version="1">MKVATQVSLKKTCQKQRNLASVLHRAHSLSNSGSSLKNFSMSDCYEKLKEIVPTIPRDRKVSRVEILQHVIDYIQDLQTALDNQNIRVKGFNENTQVAVPNRTPFSTLQSTSSSGATQLPLLMTSQPHHSLSREFVKQVMTCSSMGQKLHPYETPESSPESQPCTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BWP5</accession>
    <name>A0A9Q1BWP5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Zinc finger protein PLAG1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Pleiomorphic adenoma gene 1 protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HOLleu_24405</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Nanhai2018</strain>
            <tissue evidence="18">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BWP5"/>
    <dbReference type="OrthoDB" id="3533395at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032502">
        <property type="term" value="P:developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010557">
        <property type="term" value="P:positive regulation of macromolecule biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000425">
        <property type="entry name" value="PLAG1 like zinc finger 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000231">
        <property type="entry name" value="PLAG1 like zinc finger 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000256">
        <property type="entry name" value="PLAG1 like zinc finger 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001316">
        <property type="entry name" value="PR domain zinc finger protein 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000202">
        <property type="entry name" value="Zinc finger protein 574"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050331">
        <property type="entry name" value="Zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515:SF2">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21549">
        <property type="entry name" value="PRDM2_PR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0010">Activator</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SET" evidence="17">
        <location>
            <begin position="47"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="238"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="311"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="339"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="369"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="398"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="427"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="461"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="489"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="258"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="650"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="271"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="295"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="667"/>
            <end position="688"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00073983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00081979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ8033999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="753" mass="84797" checksum="0CFAF04234DF9C95" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BYC2</accession>
    <name>A0A9Q1BYC2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TLC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_21920</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BYC2"/>
    <dbReference type="OrthoDB" id="506011at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006634">
        <property type="entry name" value="TLC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042512">
        <property type="entry name" value="TLCD5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31898:SF1">
        <property type="entry name" value="TLC DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31898">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 136"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03798">
        <property type="entry name" value="TRAM_LAG1_CLN8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00724">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50922">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="50"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="90"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="121"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="147"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="176"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="211"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="TLC" evidence="7">
        <location>
            <begin position="45"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8034898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="262" mass="29870" checksum="08BAB99488256359" modified="2023-09-13" version="1">MELQHMDTIPEISGSPTSGFIIGSVFISLLAWITYYKLVNFIISSRNVQWNFNIVATTHALLLTSMSLVCMTYYNPPLLTKLCDSNQRPAAMVLSICLGYFIYDTSWCLIYWSEHKNVMNVVHHVITIAIISVVLSVGVSAEASTNIIFATEISTPFFMFRWFLREAQMKESLLYHVNDFIFFGLYVTFRVFIGTWYVWNVISYPDCPPLVVELSLLAISAVSWAWVPSLCKMFFYKIKKLMSSTDIQSGTSHVETKVPSLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BZG6</accession>
    <name>A0A9Q1BZG6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_19520</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8035747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BZG6"/>
    <dbReference type="OrthoDB" id="425619at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8035747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="112" mass="12328" checksum="3A83E04F95CB1E8D" modified="2023-09-13" version="1">MHDIWVADIDVDALLGYDFLQRYNCTIDAVKGEVKVCGLQHDSIDEEHTGGRVVIAKTIVVPPESESVVAARVSDVTWAQEPAVVECNAKFVKCHQIMLAKVLVDPSREVVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C1Z0</accession>
    <name>A0A9Q1C1Z0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_17899</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C1Z0"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8037151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="50" mass="5743" checksum="6B6A1C7263E289FA" modified="2023-09-13" version="1">MEPTAFVLGAQHKQWSRANKPVSHNWKKGHGRLDLNQLVGNWDVKPHLTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C2S0</accession>
    <name>A0A9Q1C2S0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Folliculin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HOLleu_17758</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Nanhai2018</strain>
            <tissue evidence="18">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Cytoskeleton</location>
            <location evidence="4">Microtubule organizing center</location>
            <location evidence="4">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
            <location evidence="5">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Lysosome membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the folliculin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C2S0"/>
    <dbReference type="OrthoDB" id="5599713at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030511">
        <property type="term" value="P:positive regulation of transforming growth factor beta receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1730">
        <property type="entry name" value="Folliculin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037521">
        <property type="entry name" value="FLCN/SMCR8_DENN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044886">
        <property type="entry name" value="FLCN_DENN_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021713">
        <property type="entry name" value="Folliculin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037520">
        <property type="entry name" value="Folliculin/SMCR8_longin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032035">
        <property type="entry name" value="Folliculin_DENN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31441:SF2">
        <property type="entry name" value="FOLLICULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31441">
        <property type="entry name" value="FOLLICULIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11704">
        <property type="entry name" value="Folliculin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16692">
        <property type="entry name" value="Folliculin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51834">
        <property type="entry name" value="DENN_FLCN_SMCR8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="13" id="KW-0458">Lysosome</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDENN FLCN/SMCR8-type" evidence="17">
        <location>
            <begin position="78"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="30"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="51"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00009987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00021824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ8037039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="567" mass="64032" checksum="198B21DB6F77E66D" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C380</accession>
    <name>A0A9Q1C380_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Microsomal glutathione S-transferase 1</fullName>
            <ecNumber evidence="5">2.5.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HOLleu_15824</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Nanhai2018</strain>
            <tissue evidence="18">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:16438"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="14">Homotrimer; The trimer binds only one molecule of glutathione.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the MAPEG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="5"/>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C380"/>
    <dbReference type="OrthoDB" id="193139at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.550:FF:000002">
        <property type="entry name" value="Microsomal glutathione S-transferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.550">
        <property type="entry name" value="Membrane associated eicosanoid/glutathione metabolism-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023352">
        <property type="entry name" value="MAPEG-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001129">
        <property type="entry name" value="Membr-assoc_MAPEG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040162">
        <property type="entry name" value="MGST1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10689">
        <property type="entry name" value="MICROSOMAL GLUTATHIONE S-TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10689:SF6">
        <property type="entry name" value="MICROSOMAL GLUTATHIONE S-TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01124">
        <property type="entry name" value="MAPEG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161084">
        <property type="entry name" value="MAPEG domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="13 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="25"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="149"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00038540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00039397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ8038403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="172" mass="19599" checksum="6F3A6D6EDB666FA1" modified="2023-09-13" version="1">MAGESGNQAEGKGSELQTLLYSAEFQVFATYVAILGLKMMVLSFLTMSERVLKGVFVTREDYEAFGDPSFSKQQIRRKTEEKDLYIERIRRCHRNDLENITVFILVGLIYLFCTKPTPDSAILHFRVFAGARIFHSLAYLLPLPQPCRLFGFLVGALATISMIIRILYQAQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C395</accession>
    <name>A0A9Q1C395_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_18431</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C395"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040396669" description="Secreted protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8037582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="86" mass="9520" checksum="5FA0CB48ACABEFDE" modified="2023-09-13" version="1">MLFGALFLVFIVLEVTHVPAETDDGTICYMLKGGNALEKHISRIACGDLENGNDIFKANHSTVFKVFESREMVSLSALMDWANKVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C504</accession>
    <name>A0A9Q1C504_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_15664</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C504"/>
    <dbReference type="OrthoDB" id="197925at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8038285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="76" mass="8542" checksum="3A96139D28A30503" modified="2023-09-13" version="1">MKWRISVPSPLSTSPTLVYKGYVYVEEDGDYWRITANGIPDHDTGEFPGSGGSSSIVEQDYDYLYLARTPTNSSFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C615</accession>
    <name>A0A9Q1C615_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Extracellular serine/threonine protein kinase FAM20C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_14130</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAM20 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8039963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C615"/>
    <dbReference type="OrthoDB" id="8583677at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024869">
        <property type="entry name" value="FAM20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009581">
        <property type="entry name" value="FAM20_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12450">
        <property type="entry name" value="DENTIN MATRIX PROTEIN 4 PROTEIN FAM20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12450:SF22">
        <property type="entry name" value="EXTRACELLULAR SERINE_THREONINE PROTEIN CG31145"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06702">
        <property type="entry name" value="Fam20C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0464">Manganese</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FAM20 C-terminal" evidence="9">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="14"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR624869-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR624869-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR624869-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF06702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8039963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="133" mass="15442" checksum="0DEEE0CD8F6D2DE4" modified="2023-09-13" version="1">MELSVFDHLIQNYDRHHFTIVVKFGIESFVVIVDNGKGFGNPWKDDVTFLAPVYQCCRFRNSTYQTLLDLTKEGSRLGKLLKFSLSQDPLYPVITDDFYEALDRRLAQFMDEIDKCITTHGKQTVLVEKMVLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C6Y3</accession>
    <name>A0A9Q1C6Y3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Lactadherin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_17019</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C6Y3"/>
    <dbReference type="OrthoDB" id="10067267at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00057">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000016">
        <property type="entry name" value="Contactin-associated protein-like 4 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000421">
        <property type="entry name" value="FA58C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050633">
        <property type="entry name" value="Neuropilin_MCO_CoagFactor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46806">
        <property type="entry name" value="F5/8 TYPE C DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46806:SF5">
        <property type="entry name" value="F5_8 TYPE C DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00754">
        <property type="entry name" value="F5_F8_type_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00231">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01285">
        <property type="entry name" value="FA58C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50022">
        <property type="entry name" value="FA58C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="F5/8 type C" evidence="7">
        <location>
            <begin position="48"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8039338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="199" mass="22489" checksum="BF7C601BF19B6CB6" modified="2023-09-13" version="1">MKLSFKLGGGLHVKEKAKTYLFELQVTLVDFADSDLYCTEHGQQEACCKRFGLNVNRDSNILLTASSSYDNKHKPDRAALFEIDTYSEPSGGIAGWATATNNVNQWIQVDFGSPRQVTGILLQGRGESGYHQWVTSFRVLFSDDAQVFYSTAVIEGTFDHETVARRFFDRPIVARYFRINPVTWFGHITLRFDLLGCDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C7N5</accession>
    <name>A0A9Q1C7N5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sulfotransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_14082</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8039925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C7N5"/>
    <dbReference type="OrthoDB" id="10374995at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48312">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48312:SF1">
        <property type="entry name" value="SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8039925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="257" mass="30553" checksum="879952FAB3C58B3D" modified="2023-09-13" version="1">MDRARAEVEEIENKEEYAALKQLEIERANKYKNVWPQETFSYPWLKEQLEIDTKDKKYVFIKDESFAIKDHVEYLPDVPTRHAFLIRHPQEVYTSFKNASVNAADFIQFSWEDYHIGDNLPYMPVKDLYKEHYDNWKYVMENLDSDPVIIDGFDLASQPEVILPKFFEKLGVPYKESYLQWNGDPELVYSTWRGSSEFVILGSKTTATSRAVESTRFEPPKYPRGTSRPEWKITDELKEYIEGAMPYYEEMYENRLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C7Q4</accession>
    <name>A0A9Q1C7Q4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Angiopoietin-1 receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_14464</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C7Q4"/>
    <dbReference type="OrthoDB" id="6130531at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.300.10">
        <property type="entry name" value="Tie2 ligand-binding domain superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0675">Receptor</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040447114" evidence="1">
        <location>
            <begin position="18"/>
            <end position="682"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8040232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="682" mass="74123" checksum="FBF6A4B61B55F0EB" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C897</accession>
    <name>A0A9Q1C897_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_13859</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8039756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C897"/>
    <dbReference type="OrthoDB" id="10051443at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF1">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="293"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="26"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="251"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="28"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="259"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8039756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="466" mass="52432" checksum="D9D1BC8D2B07B62E" modified="2023-09-13" version="1">MEYEKYVRLGKEIGLSGQDLLEFANNRETTDKKERAKLEKEAREEKDKQRQHEKDMKEYELKLLEERQRSGADGSNSQTVEVKLPKLPPFRDGRDEMDAYLFRFERFATLAGWPQSQWATSLGTLLTGQALEVYSRMPSSEANDFEKLKTALLNGYFLTREGYRQKLRSSKCGATETYAQFCDRIKGYLRRWAELSEVPKTYDGLFDLIIQEQMLWSSDKELAIFLKERSPKTASEMATLADGFKEAHVTYDGGKRKSGSSSKSNKANSIGNENQNSQEKGVKNFDSFVKEKRCYVCGKVGHLAKDCRKKFKTPGEKGAAVEISVESNASQTESNIESAALCLNVKPNWCGCHDLASDSVELSCGHKLPIVTAACAPDKCMPVCRGVINNLLVGVLRDSGCSTVVVKKDLVQSHQMVDRTQRCVLIDGTVRNVPVAQVTISSPYFEGTVEALCMDPSSIRHYYWEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C8K4</accession>
    <name>A0A9Q1C8K4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Zinc finger and SCAN domain-containing protein 10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_14325</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C8K4"/>
    <dbReference type="OrthoDB" id="427030at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000145">
        <property type="entry name" value="Zinc finger protein 574"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050888">
        <property type="entry name" value="ZnF_C2H2-type_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24406">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR CTCFL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="442"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="469"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="642"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="670"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="698"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="342"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="488"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="342"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="515"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8040119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="728" mass="83227" checksum="F70BAD68E60D5820" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C9M8</accession>
    <name>A0A9Q1C9M8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Titin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_15364</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C9M8"/>
    <dbReference type="OrthoDB" id="10265553at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26391">
        <property type="entry name" value="INACTIVE TYROSINE-PROTEIN KINASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26391:SF18">
        <property type="entry name" value="PROTEIN KINASE RECEPTOR TIE-1, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="1">
        <location>
            <begin position="4"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8040915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="109" mass="12109" checksum="CAAB566F2EB58239" modified="2023-09-13" version="1">MKNPPTNLSIGSSSVIIKWLAWDNTKDTGDPPVVGYLPYYKKTEEDDWLPGSKLSSETLMYTFNRLDPDTNYSFSVAAIRDGDGGEGPKSPQLNVSTICKGKTFLRPEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CA83</accession>
    <name>A0A9Q1CA83_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_15520</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8041033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CA83"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12690">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8041033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="90" mass="9981" checksum="16716DA54AAAFC40" modified="2023-09-13" version="1">MTARTGGFQFRKDEGAKPVEIAEGLEDTVEEVVVEPKEGDSGISSGLLEIEMAKGKVLFAGDSITRFTERELCKRDRRKRVRICLPGARI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CAG8</accession>
    <name>A0A9Q1CAG8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phosphatidylinositol-3-phosphatase SAC1</fullName>
            <ecNumber evidence="1">3.1.3.64</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Phosphatidylinositol-4-phosphate phosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Suppressor of actin mutations 1-like protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_11814</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate) + H2O = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:55652"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
            <dbReference type="ChEBI" id="CHEBI:58178"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="3">
            <dbReference type="Rhea" id="RHEA:55653"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3-phosphate) + H2O = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:12316"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
            <dbReference type="ChEBI" id="CHEBI:58088"/>
            <dbReference type="EC" id="3.1.3.64"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="2">
            <dbReference type="Rhea" id="RHEA:12317"/>
        </physiologicalReaction>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.64" evidence="1"/>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CAG8"/>
    <dbReference type="OrthoDB" id="405996at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004438">
        <property type="term" value="F:phosphatidylinositol-3-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043812">
        <property type="term" value="F:phosphatidylinositol-4-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002013">
        <property type="entry name" value="SAC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45662">
        <property type="entry name" value="PHOSPHATIDYLINOSITIDE PHOSPHATASE SAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45662:SF2">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-3-PHOSPHATASE SAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02383">
        <property type="entry name" value="Syja_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50275">
        <property type="entry name" value="SAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="543"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="569"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="domain" description="SAC" evidence="8">
        <location>
            <begin position="139"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00036631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00036807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00040795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00041396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00041911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8041094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="610" mass="70304" checksum="E1AB055F9B9BF7CB" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CGG5</accession>
    <name>A0A9Q1CGG5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BESS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_07754</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CGG5"/>
    <dbReference type="OrthoDB" id="10051975at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8044876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="138" mass="15821" checksum="FF1235C9B538E8AF" modified="2023-09-13" version="1">MLTEKFTKFIFSSQKRYTDPTEELELLGEADSDDVKCLDVGIPQLISQTGPTPPKKKKESMEEKEMKIMEELLKSLTKVADNRETLRSEDDNDVFGVFVASELRKIKDEQVKNVTKMKIHTVLFDGMQDYLAKSKALE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CGJ2</accession>
    <name>A0A9Q1CGJ2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_07307</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CGJ2"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="374"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="467"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="557"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="777"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="111"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="142"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="174"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="184"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="374"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="406"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="435"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="467"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="500"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="564"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="794"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="824"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="845"/>
            <end position="862"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8044530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="862" mass="92515" checksum="6476B0473293FA75" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CH90</accession>
    <name>A0A9Q1CH90_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Alpha-ketoglutarate-dependent dioxygenase alkB-like 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_08273</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the alkB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CH90"/>
    <dbReference type="OrthoDB" id="412814at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.590">
        <property type="entry name" value="Alpha-ketoglutarate-dependent dioxygenase AlkB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027450">
        <property type="entry name" value="AlkB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037151">
        <property type="entry name" value="AlkB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032862">
        <property type="entry name" value="ALKBH6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46030">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT DIOXYGENASE ALKB HOMOLOG 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46030:SF1">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT DIOXYGENASE ALKB HOMOLOG 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13532">
        <property type="entry name" value="2OG-FeII_Oxy_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="9">
        <location>
            <begin position="149"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8045292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="287" mass="32313" checksum="A38486E5405D474E" modified="2023-09-13" version="1">MAATPVRDIASFKVSKAPDTVYYIPEFISHEEEKYLIDHVRICVNFDVFGMLSSYLNAAHPSSHLETCLFCTKTKMDSTLKQETAKLGQVNDITLQYCKKQFAESVMIPGCGTPRGLPHPKGMIAEKLPQWLEIYANKIAKLGVFEERVPNHVLVNEYKPQQGIMPHEDGPLFYPVVTTINLGSHSVLQFYQHSSKQDGDSQSENHSEPIFTLLLKPRSLLILKDSVYTSYLHGIAECVEDTVDDNVANLSSTENKVGEVLTRGVRISLTIRFVPKTLKMKFMFGSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CHK8</accession>
    <name>A0A9Q1CHK8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Testis-specific serine/threonine-protein kinase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HOLleu_08434</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Nanhai2018</strain>
            <tissue evidence="15">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CHK8"/>
    <dbReference type="OrthoDB" id="541276at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050321">
        <property type="term" value="F:tau-protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000658">
        <property type="entry name" value="Protein CBG12184"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF102">
        <property type="entry name" value="TESTIS-SPECIFIC SERINE_THREONINE-PROTEIN KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6" id="KW-0221">Differentiation</keyword>
    <keyword evidence="15" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10" id="KW-0744">Spermatogenesis</keyword>
    <keyword evidence="15" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="28"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="287"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="313"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ8045423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="371" mass="42586" checksum="29891D96D41C26CD" modified="2023-09-13" version="1">MSDNYELKGTTSVRKALNEEATLSRRGYTVGVLLGEGSYAQVYSCVDDRRRRKCAMKIVNRRKAPADFLLKFLPRELKLLKKFKHCNIVQLYEIFEFGSKVYISMELAGHGDLLDYIRLRRYLSEDTSRNFFRQLLYGIQHLHKMNIVHRDLKCENILLTDQNQIKITDFGFARETGENEFISTFCGSAAYAAPEILQGQAYRGVNYDVWSLGIVLYIMVCGSMPFDDSNINKMIQAQLSQKIPFSKKREFTDNFKALIFAILQPQPKRRLSIDDILRHGWVDQPPDRIQGNLMDSNQKNIPRRRRPNVPAEAVKENRTDESQVKNCQQKKGVTQIDGDQDTSVSSRRRAAPIQTVAAPETGESANKLAKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CII9</accession>
    <name>A0A9Q1CII9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Pre-mRNA-processing factor 39</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_09083</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the PRP39 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CII9"/>
    <dbReference type="OrthoDB" id="10265668at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000243">
        <property type="term" value="C:commitment complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030627">
        <property type="term" value="F:pre-mRNA 5'-splice site binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000395">
        <property type="term" value="P:mRNA 5'-splice site recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000063">
        <property type="entry name" value="Pre-mRNA processing factor 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059164">
        <property type="entry name" value="HAT_PRP39_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17204">
        <property type="entry name" value="PRE-MRNA PROCESSING PROTEIN PRP39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17204:SF5">
        <property type="entry name" value="PRE-MRNA-PROCESSING FACTOR 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23241">
        <property type="entry name" value="HAT_PRP39_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23240">
        <property type="entry name" value="HAT_PRP39_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="131"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="488"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="504"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="532"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="542"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="559"/>
            <end position="581"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8045956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="601" mass="70300" checksum="11DB62C3F0A80581" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CIP5</accession>
    <name>A0A9Q1CIP5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_08685</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CIP5"/>
    <dbReference type="OrthoDB" id="9973206at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8045640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="244" mass="28589" checksum="E93E19FA2FC7A743" modified="2023-09-13" version="1">MAHSFYVTLPSNSSPLLYPDNTVTHYRVKLSQPISLEGQWEVGLAEMIYPHQWYNIDEECEYSYTVNGHQWWRKQIDPGYYGSMKEILELLETNYPEQIQYLYHEKTRRLEIRLGEGAQVRIKGRLAGLLGFQTEAPTVTQSTTLDRPLDLRQPHNLFVYCDIVEPRAVGHTRVPLLRVVNVRQRYGEDVSVIFTNIHYQSVKQKYFDTIEIDIRDSVGRKVPFVRGNVIVTLHFRLKRASQFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CJJ0</accession>
    <name>A0A9Q1CJJ0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Sec1 family domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_09025</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the STXBP/unc-18/SEC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CJJ0"/>
    <dbReference type="OrthoDB" id="10251230at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.60:FF:000002">
        <property type="entry name" value="Sec1 family domain containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2060:FF:000002">
        <property type="entry name" value="sec1 family domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.830.10">
        <property type="entry name" value="Syntaxin Binding Protein 1, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043154">
        <property type="entry name" value="Sec-1-like_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043127">
        <property type="entry name" value="Sec-1-like_dom3a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001619">
        <property type="entry name" value="Sec1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027482">
        <property type="entry name" value="Sec1-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036045">
        <property type="entry name" value="Sec1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11679">
        <property type="entry name" value="VESICLE PROTEIN SORTING-ASSOCIATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00995">
        <property type="entry name" value="Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005715">
        <property type="entry name" value="VPS45_Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56815">
        <property type="entry name" value="Sec1/munc18-like (SM) proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8045908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="644" mass="72911" checksum="29789DAEE9E47D6A" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CJV0</accession>
    <name>A0A9Q1CJV0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase aspartic putative domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_04855</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CJV0"/>
    <dbReference type="OrthoDB" id="10064741at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005312">
        <property type="entry name" value="DUF1759"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331:SF5">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03564">
        <property type="entry name" value="DUF1759"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8046240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="653" mass="73627" checksum="9540489B791C510E" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CKJ9</accession>
    <name>A0A9Q1CKJ9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Solute carrier family 23 member 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_06190</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CKJ9"/>
    <dbReference type="OrthoDB" id="1641903at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006043">
        <property type="entry name" value="NCS2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11119">
        <property type="entry name" value="XANTHINE-URACIL / VITAMIN C PERMEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00860">
        <property type="entry name" value="Xan_ur_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040129012" evidence="7">
        <location>
            <begin position="19"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="99"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="187"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="214"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="244"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="282"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8047237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="417" mass="44805" checksum="702F35DDEA91BAEB" modified="2023-09-13" version="1">MWIICAILTACDVFPSDSSVYGYAARTDLKTENLKNSPWISFPYPANKMTWETTLEQTVDTMKCISLQRGLGLGACDPDSVLDSNAGDSVSDSDSVGQWGWPTFNIAAIIGTFSAVVVSILDSVGDYHACSQVAHVPTPPDHAINRGIGVEGVGGLMASVWGTGIGVVSYSGDIALLSLTKAASRSILIGSGSIFILVGFFTKLIALFNGIPKPVLAASSIQVGMVSSIGLSNLSSVKMDSSRNLLVVGFSLFVAIGVPEYLGRHPGSIDTGSQTVDNILEALLLNGMLVGAVISTFLDLVIPGVGKILSILPQIFTKIFGWGLQPPAPTSYTFVNGKWTPEERGANWRELVETDKDDANYIESSFHEAYRVYDLPFGGKWLTKQKWTRFIPFSPTFQSDTKVDADCQPHFELKASE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CKL9</accession>
    <name>A0A9Q1CKL9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_05158</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CKL9"/>
    <dbReference type="OrthoDB" id="10053045at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000020">
        <property type="entry name" value="Transposon Tf2-6 polyprotein-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041577">
        <property type="entry name" value="RT_RNaseH_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF15">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17919">
        <property type="entry name" value="RT_RNaseH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="5">
        <location>
            <begin position="471"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="8"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8046478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="773" mass="86555" checksum="AA979535028DBE9B" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CLM0</accession>
    <name>A0A9Q1CLM0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Metalloproteinase inhibitor 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_06094</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protease inhibitor I35 (TIMP) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CLM0"/>
    <dbReference type="OrthoDB" id="10018662at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008191">
        <property type="term" value="F:metalloendopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002020">
        <property type="term" value="F:protease binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051045">
        <property type="term" value="P:negative regulation of membrane protein ectodomain proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.370.10">
        <property type="entry name" value="Tissue inhibitor of metalloproteinase-1. Chain B, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001134">
        <property type="entry name" value="Netrin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001820">
        <property type="entry name" value="TIMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008993">
        <property type="entry name" value="TIMP-like_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027465">
        <property type="entry name" value="TIMP_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11844">
        <property type="entry name" value="METALLOPROTEASE INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11844:SF33">
        <property type="entry name" value="TISSUE INHIBITOR OF METALLOPROTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00965">
        <property type="entry name" value="TIMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00206">
        <property type="entry name" value="NTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50242">
        <property type="entry name" value="TIMP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50189">
        <property type="entry name" value="NTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0481">Metalloenzyme inhibitor</keyword>
    <keyword evidence="4" id="KW-0483">Metalloprotease inhibitor</keyword>
    <keyword evidence="5" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040261290" evidence="9">
        <location>
            <begin position="26"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="NTR" evidence="10">
        <location>
            <begin position="26"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="242"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="261"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR601820-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8047170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="312" mass="36167" checksum="200259645114FDD2" modified="2023-09-13" version="1">MEWGIKILPTFLVLLATTGKHIIEACQCDEPKHPQERFCRAEYGIKLPQHNFPTKDNRPFYIMEVIIGRIVEKIEEYDDDFPQIPFVPHIPGDGIPVPSTTREPELLDELPLDRLPILRKVQAPKEPEVFPPMIVNDGPMHGFYPGMMAWRGKLKYVVQVEKVFKGKERILKNSKVVIETDLTSSLCGRTELEVEERYLISGSYDGDKMSLYLCDWVERTTELTKTQRRGLRHFYSKYCGKCEIQTGIFYFPGDPESKRVCSYNSHLYSDVDCEAKFSSCIQRSNGTCRWYKGKDYKTCKKLSEMKQGRTPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CNX3</accession>
    <name>A0A9Q1CNX3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_00301</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CNX3"/>
    <dbReference type="OrthoDB" id="2194416at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040460895" evidence="2">
        <location>
            <begin position="27"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="127"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="232"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="36"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="236"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8048125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="287" mass="32553" checksum="6C61B708EC55C14C" modified="2023-09-13" version="1">MGQQHTNKRFFPFGGIWACFIYGAFIDDPVSSPKSNNNNNNNNTNTNTNTNNNNTNTNTNNNNNNNNNNGELTSADSTSFTEASHWKTSFTESSHWKNQRGGSYKSWFPNPCMMEKELNDLTIPAQTSPGIKRSAPAAASLGRTGEKWATETTKGRKKWTQDENKEVMRCYFLSDPNQRGYRKRMHEIWQKRNPSTPVTEQRLADQRNVINRAGILTTVEVESIQREVCCEEQPVEKNQQPASQQLTPNDMPASEPERCPKMPPEGTLAREIIDEMVSTETERTPKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CT85</accession>
    <name>A0A9Q1CT85_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Fucosyltransferase</fullName>
            <ecNumber evidence="11">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_03951</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Endomembrane system</location>
            <topology evidence="10">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Golgi apparatus</location>
            <location evidence="11">Golgi stack membrane</location>
            <topology evidence="11">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the glycosyltransferase 10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="11"/>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CT85"/>
    <dbReference type="OrthoDB" id="427096at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046920">
        <property type="term" value="F:alpha-(1-&gt;3)-fucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11660:FF:000002">
        <property type="entry name" value="Alpha-(1,3)-fucosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11660">
        <property type="entry name" value="Glycosyl transferase family 10, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055270">
        <property type="entry name" value="Glyco_tran_10_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031481">
        <property type="entry name" value="Glyco_tran_10_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001503">
        <property type="entry name" value="Glyco_trans_10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038577">
        <property type="entry name" value="GT10-like_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11929">
        <property type="entry name" value="ALPHA- 1,3 -FUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11929:SF145">
        <property type="entry name" value="ALPHA-(1,3)-FUCOSYLTRANSFERASE FUT-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17039">
        <property type="entry name" value="Glyco_tran_10_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00852">
        <property type="entry name" value="Glyco_transf_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Fucosyltransferase N-terminal" evidence="13">
        <location>
            <begin position="45"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Fucosyltransferase C-terminal" evidence="12">
        <location>
            <begin position="158"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00060399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF17039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ8050661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="405" mass="47768" checksum="559FD0A050D6DD4C" modified="2023-09-13" version="1">MSTPDNTARHILHKTTTKLPSIRKNYDFVAFKKKPGWHFVDFPCHYVCEEHAASVTVHFTTNASDIVGANAVFFSTGPFTLEEWEALQYYRNPGQIWIFATQEPASIVPDFLPPKVYRYNTYNWSFTFHSTSDIHGAYGWYTPYTQPPSTLHPLNWHKEKPKLAAWVSSRHCEGLGWNRTQFVIDLGKFIPIDTYGKCGNTTLRKNAAIAREQFKIYKFHLSLENSCCSEYLSEKVWNAFENWESVPIVIGGTKQEYERLAPPHSFIHADDFGSMKELADYIRKVSEDEHLYNEYFDWRNKGYVKQQSFEFRFPVFRQGVCKVVDRMEKIKREDNDMFDPYGPDWFGSCYRCGEHSWIRNYTVWWRRTIFAQDWEDNRILHWMDNETKQAVNSLSAKRLSQHKTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1GY71</accession>
    <name>A0A9Q1GY71_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_30006</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8027914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1GY71"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8027914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="83" mass="9563" checksum="1967B19A2D8F055E" modified="2023-09-13" version="1">MSKPCKHDNSRRITVRDLTLDMWMIPTIFCGGRRFFSNTPSTSCISPESLPARRHPTLNYRERVNANDPLKFKPTRNGVTLLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H1V9</accession>
    <name>A0A9Q1H1V9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_25483</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H1V9"/>
    <dbReference type="OrthoDB" id="2136082at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="99"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="152"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8032067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="168" mass="18795" checksum="480E99295E2137CE" modified="2023-09-13" version="1">MRGHKQRKDQMDQFTPTTTEVVSSEENESDVDYAARTIQSAVRGHWSRKDQLTKMSMTDSETESVHSSRSRASTSHKGHSSHKRLDSEGDSDDNIMVTPSSSRKGSMRGSRPSSAHSSSKKRSSSSSISSDKQRNKVSKSKFYEQQVESFKKASAAAKNSKMSRYMFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H4G1</accession>
    <name>A0A9Q1H4G1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">Cysteine dioxygenase</fullName>
            <ecNumber evidence="3 11">1.13.11.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_22139</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-cysteine + O2 = 3-sulfino-L-alanine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20441"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:61085"/>
            <dbReference type="EC" id="1.13.11.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="11">Binds 1 Fe cation per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Organosulfur biosynthesis; taurine biosynthesis; hypotaurine from L-cysteine: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the cysteine dioxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.20" evidence="3 11"/>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H4G1"/>
    <dbReference type="OrthoDB" id="543511at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017172">
        <property type="term" value="F:cysteine dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019448">
        <property type="term" value="P:L-cysteine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042412">
        <property type="term" value="P:taurine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd10548">
        <property type="entry name" value="cupin_CDO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000045">
        <property type="entry name" value="Cysteine dioxygenase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010300">
        <property type="entry name" value="CDO_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12918">
        <property type="entry name" value="CYSTEINE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12918:SF1">
        <property type="entry name" value="CYSTEINE DIOXYGENASE TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05995">
        <property type="entry name" value="CDO_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11 12" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="8 10 11" id="KW-0408">Iron</keyword>
    <keyword evidence="4 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0883">Thioether bond</keyword>
    <feature type="binding site" evidence="10">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="cross-link" description="3'-(S-cysteinyl)-tyrosine (Cys-Tyr)" evidence="9">
        <location>
            <begin position="93"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR610300-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR610300-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU366010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8035052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="205" mass="23766" checksum="4C69F2D12AA99D5D" modified="2023-09-13" version="1">MERRFIKSPSSLDELIESLHVLFESDKVNVEEVQEVMENYKSNEDHWGKFADYDHNRYTRNLVDEGNGKFNLMLLCWGESQGSSIHSHSNAHCFMKILDGSLTETLYSWPEKGTKKKPMQQLASNDYNENEVAYICDEKGLHRVENKSHTDTACSLHLYSPPFDSCQCFDQRTSKAYSTKVTFWTKYGKRTPFNVSEFPRLSSGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H640</accession>
    <name>A0A9Q1H640_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_24140</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="7553019at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005312">
        <property type="entry name" value="DUF1759"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008042">
        <property type="entry name" value="Retrotrans_Pao"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331:SF4">
        <property type="entry name" value="PEPTIDASE S1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03564">
        <property type="entry name" value="DUF1759"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05380">
        <property type="entry name" value="Peptidase_A17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="2">
        <location>
            <begin position="318"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase catalytic" evidence="3">
        <location>
            <begin position="1272"/>
            <end position="1440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8033781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1542" mass="175029" checksum="FB461D63618ACC2E" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H776</accession>
    <name>A0A9Q1H776_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Hypoxia up-regulated protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_22020</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H776"/>
    <dbReference type="OrthoDB" id="10262720at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034663">
        <property type="term" value="C:endoplasmic reticulum chaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030968">
        <property type="term" value="P:endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903298">
        <property type="term" value="P:negative regulation of hypoxia-induced intrinsic apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10230">
        <property type="entry name" value="ASKHA_NBD_HSP70_HYOU1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45639">
        <property type="entry name" value="HSC70CB, ISOFORM G-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45639:SF3">
        <property type="entry name" value="HYPOXIA UP-REGULATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040310906" description="Hypoxia up-regulated protein 1" evidence="10">
        <location>
            <begin position="23"/>
            <end position="1120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="578"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="984"/>
            <end position="1120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="593"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="612"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="631"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="644"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="672"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="689"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="703"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="714"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="747"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="984"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="994"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="1012"/>
            <end position="1028"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="1047"/>
            <end position="1062"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="1069"/>
            <end position="1078"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="1086"/>
            <end position="1097"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1098"/>
            <end position="1108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8034965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1120" mass="126160" checksum="CD62C0A302748F81" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H7M1</accession>
    <name>A0A9Q1H7M1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_16174</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H7M1"/>
    <dbReference type="OrthoDB" id="6493944at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039470">
        <property type="entry name" value="Nuc_deoxyri_tr2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36300">
        <property type="entry name" value="RAW, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36300:SF1">
        <property type="entry name" value="RAW, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15891">
        <property type="entry name" value="Nuc_deoxyri_tr2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040462326" evidence="2">
        <location>
            <begin position="20"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="334"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8038682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="415" mass="47061" checksum="884FF959A2E26BD1" modified="2023-09-13" version="1">MYFVICLNLLILLSTLLLARELLDLKRGHAFLCDLVERQCLPIFDDLETAIHCSAKVLKQGVDISQLSKEDGAQPVRNPHIRVSERLINIKETFRSYQSQETGKLPVSDTKFALRVLSGKDTSFPALQQIISKGSLIENNFEFDFDQFCCLLTEFEQLQPYRRAATRWLYSARRLLLRGWSAPVPHPEPEMKRDIFLGGSCRDTEWRKSVIPLFRKEGFTMYDPQQSHWNLRFLPLEAHAKENSEYMLYVISDTSRGIASMVEVAHYIGQKRKVVLCIQHLQEGCEIDGEKLSSRSVKDYNRGRCYLADLASRQGTPIFDNVVEAAKALSTRIHRDRSRTEQVFPPQHGTVSQEDSKIQKTGGSHPLESGSVSHFKEESSYAGRVTSHSEDASIQVGSGTVHRVNDSPETENGVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H7R5</accession>
    <name>A0A9Q1H7R5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_20120</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H7R5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8036209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="111" mass="12374" checksum="A5AA7903DFBD0D43" modified="2023-09-13" version="1">MDKPPPYAPSPQQGYPPPQQGYPPPQQGYPPPQQGYPPPQQGYPPPQQGYYPPPQQQVVVVGQQPAPATNTVVVTTNTVHHKPGVNHCLHCIISIFFWPWLLVWLCLCICE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H8P3</accession>
    <name>A0A9Q1H8P3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_20385</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H8P3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="1">
        <location>
            <begin position="3"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8036421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="198" mass="23150" checksum="A4FF39B4123A074F" modified="2023-09-13" version="1">MFPKLHESVKTKTYYELDSGKHFSLNCTADHAEEFKWQQETYKGTKVVSNSSTLHINSVSYRDQGSYYCIVKGKKDYDTSAAQVLVMKDYLQFLVEVLTNNSLTNNVSTEIVNTLRNEHLNVTQPEIGGEGEIFKLYVFKKILKKTQPSKVYELIKDKLDSLRDKLRTWDYEIIRTGKICRFLDLSYIHYFDVCMLQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H938</accession>
    <name>A0A9Q1H938_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_18420</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H938"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8037574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="55" mass="6420" checksum="2D29EC7752E8D33C" modified="2023-09-13" version="1">MMDDNNLYTQCRNGVRQFITSVQCQWLGITSQQRLDSMKCYDISLITVVELPFIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H952</accession>
    <name>A0A9Q1H952_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DDE-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_17847</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H952"/>
    <dbReference type="OrthoDB" id="10043687at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15517">
        <property type="entry name" value="PHD_TCF19_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050863">
        <property type="entry name" value="CenT-Element_Derived"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004875">
        <property type="entry name" value="DDE_SF_endonuclease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19303:SF74">
        <property type="entry name" value="POGO TRANSPOSABLE ELEMENT WITH KRAB DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19303">
        <property type="entry name" value="TRANSPOSON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03184">
        <property type="entry name" value="DDE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE-1" evidence="2">
        <location>
            <begin position="177"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="399"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="466"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="399"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="472"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8037108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="575" mass="64390" checksum="35BCD72341CF7F60" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H979</accession>
    <name>A0A9Q1H979_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Serine/threonine-protein kinase 19</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_17641</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the STK19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8036961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H979"/>
    <dbReference type="OrthoDB" id="10261701at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046579">
        <property type="term" value="P:positive regulation of Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018865">
        <property type="entry name" value="STK19-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15243">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15243:SF0">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10494">
        <property type="entry name" value="Stk19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00093458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8036961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="251" mass="28643" checksum="8459CA687896ACB5" modified="2023-09-13" version="1">MKRSNQRNLSYGLGKRRKKATAQDGNNNKRTEDDPISTEAALTFFHAMFNPEKFDGRVPPIIFTHQLYSVVNNKTQVDREVNELKEKKEVKVFCLGSETETCIVFTKDYKDCLRKNDRCPSAITEKFISKVVDGSKDLTFNKSTMTQFGFPEQEITQLVNGGLLTVRALGNWWLAIPGAGVFMKCYRKGREAILRTIRTSRFKEILLQDLEGRKLQAVQPLGMKYHIYDILGAESVTKIQTTSGVLLKYDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H9R6</accession>
    <name>A0A9Q1H9R6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_15617</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H9R6"/>
    <dbReference type="OrthoDB" id="273070at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8038249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="146" mass="16975" checksum="9E0D0A46C034F219" modified="2023-09-13" version="1">MSDHNVVIGIFNTALQYVRKPQRTVFLYNKANWKDISIAVKQLSIDSFIQSRIEGILNQLVPQKTSKSRFHLPWITRDVKTLMNRRDKLYIRAVRSKSPNDWLKFKSARTTAKQAIRNSHRSYVSEIIGEGLKDNPKVFWSDQGSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HAC0</accession>
    <name>A0A9Q1HAC0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptidase A2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_12730</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HAC0"/>
    <dbReference type="OrthoDB" id="6142091at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF13">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="178"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="422"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="78"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="179"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="197"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="422"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="446"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="466"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="490"/>
            <end position="504"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8041817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="617" mass="69641" checksum="66B425B2A0AAD0CC" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HAE6</accession>
    <name>A0A9Q1HAE6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">IgGFc-binding protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_15767</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HAE6"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035234">
        <property type="entry name" value="IgGFc-bd_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46534:SF1">
        <property type="entry name" value="IGGFC-BINDING PROTEIN N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46534">
        <property type="entry name" value="IGGFC_BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17517">
        <property type="entry name" value="IgGFc_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IgGFc-binding protein N-terminal" evidence="1">
        <location>
            <begin position="174"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF17517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8038368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="472" mass="52220" checksum="213E5E1385147DA4" modified="2023-09-13" version="1">MEFHASLTMSPITITLILSYVVSSFTLAGSLDAPICYKKHALNEKTTRFTRTVCEEPANAKKSEIEVLKSDFSLSALIVERQIYIGTPSKTTGASGRISFPSQNISDIYFTLKSGQGRFFEVPSGYQKPTRDDSGLLLNTTIVVAADEAVNVYAYQGCAHAYGKQASAFRVKNIKNLGTDYWVLTYFRSYSHLGIVAVKDNTSLSISFNHSDGGSEMNTQDILLNKYDTYYLVLPFDATGTHITSDEKISVVAGVRSIILPKGSKNTEPFCETLEPVSDWGKSFSTVHFLGPQKGNDYIIKFISAENNNTVNWKQGAYQNSTILQQGESQTLLVPANYTNVLEINASKDILLAQFATKGNPPITNPAMTLIPPHENGTEEEIVFPVFDLTYKQNATNYLTVWMPASAKRSDLSLDGMRYVNWREFAEDGSGWKILQTALKEGSHVLMINKGLKVRAIVFAAEHLRSVAYPVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HB30</accession>
    <name>A0A9Q1HB30_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_16340</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HB30"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF13">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="78"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8038806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="268" mass="32196" checksum="FC6D36BE64404587" modified="2023-09-13" version="1">MPFNLDKFVASPSVEELDSLKKSEVVKVAKHYGIEFQPLMRKDEIKRYVLEYLVDESFLPSTVLETAITVPTDNTFELKRLEMEMNKEVRLKEMEREREKEEREMQMQMQKEKEEREMQMQMQREEKAREHEFWLKQLELGVIKGSDPKIGLDTGGFDVSKHAKFVPKFQEDNVEKFFNHFEKLGEQLKWPRDKWSILIQSNFTGKAQEVYSALSIEDSMDYDKVKKAILQAYELVPEAYRQKFRKYRKADTQTYVEFAYQKERHFDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HBJ0</accession>
    <name>A0A9Q1HBJ0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ATP-binding cassette sub-family F member 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_17133</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. ABCF family. EF3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HBJ0"/>
    <dbReference type="OrthoDB" id="2110130at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03221">
        <property type="entry name" value="ABCF_EF-3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000471">
        <property type="entry name" value="ATP-binding cassette, sub-family F (GCN20), member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032781">
        <property type="entry name" value="ABC_tran_Xtn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050611">
        <property type="entry name" value="ABCF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000355">
        <property type="entry name" value="ribo_prot_ABC_F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211:SF14">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY F MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211">
        <property type="entry name" value="ATP-BINDING TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12848">
        <property type="entry name" value="ABC_tran_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="329"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="689"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="620"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="45"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="101"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="116"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="184"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="209"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="229"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="251"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="620"/>
            <end position="654"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8039418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="907" mass="102114" checksum="F9D466A7982DBF91" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HCY1</accession>
    <name>A0A9Q1HCY1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Dihydroxyacetone phosphate acyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_07680</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the GPAT/DAPAT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HCY1"/>
    <dbReference type="OrthoDB" id="10255570at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004366">
        <property type="term" value="F:glycerol-3-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016287">
        <property type="term" value="F:glycerone-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008611">
        <property type="term" value="P:ether lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019432">
        <property type="term" value="P:triglyceride biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07993">
        <property type="entry name" value="LPLAT_DHAPAT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022284">
        <property type="entry name" value="GPAT/DHAPAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045520">
        <property type="entry name" value="GPAT/DHAPAT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041728">
        <property type="entry name" value="GPAT/DHAPAT_LPLAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12563:SF17">
        <property type="entry name" value="DIHYDROXYACETONE PHOSPHATE ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12563">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19277">
        <property type="entry name" value="GPAT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000437">
        <property type="entry name" value="GPAT_DHAPAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 9" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="8">
        <location>
            <begin position="126"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="657"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="669"/>
            <end position="679"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR000437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8044817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="679" mass="77634" checksum="814150655E0F90FF" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HD83</accession>
    <name>A0A9Q1HD83_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Heat shock factor 2-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_15371</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HD83"/>
    <dbReference type="OrthoDB" id="10065854at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039584">
        <property type="entry name" value="HSF2BP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15434">
        <property type="entry name" value="HEAT SHOCK FACTOR 2-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15434:SF2">
        <property type="entry name" value="HEAT SHOCK FACTOR 2-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0346">Stress response</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8040921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="237" mass="26481" checksum="8F4490C300DD277F" modified="2023-09-13" version="1">MLTTRKFCVLELMYLSFFFILFHSQNKLKTQAEMTEMSNQLSLQSEYCASMGAVCCTLLWKASQQEDIIPHLLAGSQMKSFIPVVSFTLESFLATYEGEMPENMNEEIQFVLSLVGIVTNVAASAVGREFLSKSSHCKTLVESFSHLLAKPFSKHLIRLKGLAVMSLYNLSINQMGLKVLTSTKGLVPLLTWLLSVENSEEMRLHTLLLLHSLISEPNNIKLLLEVKEGVSLPSAAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HEJ7</accession>
    <name>A0A9Q1HEJ7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_10475</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HEJ7"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8043409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="89" mass="10351" checksum="E2E458FEB0F201A0" modified="2023-09-13" version="1">MYMHYGKRKNPIVFDGGQRSSGVKKQNFKNSQDRLMGSPEGKKQIFPRIPKIIILSHQMSKCKISQEFPRSNMNLKLFFQHVKIAALDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HFA7</accession>
    <name>A0A9Q1HFA7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Snaclec 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_09878</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8042976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HFA7"/>
    <dbReference type="OrthoDB" id="538816at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050111">
        <property type="entry name" value="C-type_lectin/snaclec_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022041">
        <property type="entry name" value="Methyltransf_FA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22803">
        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12248">
        <property type="entry name" value="Methyltransf_FA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040280774" evidence="2">
        <location>
            <begin position="26"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="3">
        <location>
            <begin position="37"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="3">
        <location>
            <begin position="228"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8042976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="328" mass="36721" checksum="01052904B422F7D4" modified="2023-09-13" version="1">MGRFLSQNFFLLVIFCDVWLTSVGGVSMDCATDETAYAGSCYFVGNTSHSQRAARAFCIDRGMDLVTIDYEEENEFIQNEIPAEADAWTGLASGLYGGGGGGFSSSSGVWQSFQVLPAGTPYQEFSVDTDGSDARVALMEDIEGGKRYEIALGAKNDTKVTISRCDGIQPCRVKKRRIIRKGIFGTPRMERAFSVKCWRGRILIQTIDKNGAKLNMTFRDDSPIPCNYIGFASGSGSFISWGYWHRFAWANGDSVKYLNWGRNAPKETHGDRCVTIAKKLGSWNDEPCNRRHPFICESYLQQEDTYYDKLSSSFVPSSSYYDFYDWIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HHI3</accession>
    <name>A0A9Q1HHI3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_08035</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HHI3"/>
    <dbReference type="OrthoDB" id="5989970at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8045103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="353" mass="40137" checksum="6C0F87B6BAA3C9E5" modified="2023-09-13" version="1">MVYEIDDFNNKPPKEKLPYNIERQLAVVGQPHKTPCRLYIAIPYFKAHYYIRTKYECIVTELVAKARWLHGPQFLWQSREQWPEQPRLENLISDPEIKAKSDESCLVTSQGGKSIIDRLLESRSDWYSLKKDVAWIYLRHKILNQVIPELGQPLSVQELEAAEVAIVKYIQQGMKGVTFSASGFNVEKSSAVNKLDPYVSRHGILCVGGPLHNAQPDNRSKHPYILPKEAYVCSLIVKDIHVHSGHADRTTLATLQLHPGGTLAASIKIKGVTVMASVIIRLCADLELFKKAEMKAREILQHEFILVIEISLLVTYIRREAASERNFDIKFETKMLLIWALLAIPSSITDLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HIE3</accession>
    <name>A0A9Q1HIE3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase A2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_05636</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HIE3"/>
    <dbReference type="OrthoDB" id="10064107at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000130">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon opus-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8046830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="144" mass="16165" checksum="9BA51103CEC0474D" modified="2023-09-13" version="1">MIDTGAEVSVVPPRREDRKRGSTEFSLQACNKSRIKTIGMRSLTLNLGLRRACQWIFIIADVKTPILGVDFLHHFGFLVDIRRYRLIDSTTQMNVKGHRANTTSLSPSFCSISPTSPFHELLTQFPSVTKPCFLEGTVNSVLIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HII1</accession>
    <name>A0A9Q1HII1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Alpha-2,8-sialyltransferase 8B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_00107</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 29 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8047969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HII1"/>
    <dbReference type="OrthoDB" id="10264956at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003828">
        <property type="term" value="F:alpha-N-acetylneuraminate alpha-2,8-sialyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006491">
        <property type="term" value="P:N-glycan processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009311">
        <property type="term" value="P:oligosaccharide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1480.20">
        <property type="entry name" value="Glycosyl transferase family 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001675">
        <property type="entry name" value="Glyco_trans_29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050943">
        <property type="entry name" value="Glycosyltr_29_Sialyltrsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038578">
        <property type="entry name" value="GT29-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11987">
        <property type="entry name" value="ALPHA-2,8-SIALYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11987:SF36">
        <property type="entry name" value="SIA-ALPHA-2,3-GAL-BETA-1,4-GLCNAC-R:ALPHA 2,8-SIALYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00777">
        <property type="entry name" value="Glyco_transf_29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8047969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="282" mass="32275" checksum="207D137B17AA5C09" modified="2023-09-13" version="1">MKHYFQVFTLTCFSAIISLFGYSYLSGTTPTVRYHEDIKISVAYDLYKLCDKICDRSMKNCFDTPLTILVYQNASLDRMKFRQVFSPYVRNRSIAEVLKEHVDKKLERRTYCPVTDIDSVYGTLEKQGRCAIVGNGGILLKSGCGREIDSNNFVIRANFAGLENYSDDVGKKTSLMMINDATLTNMYNTLVAQKGGKDKFDTMMRYIKLHLNDTVIWFAKSTESHRSAARLKKIAELFAKYELRPKLAYSVQQISSAAARAVEATKLSFLWCRYFSCCRSHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HJ28</accession>
    <name>A0A9Q1HJ28_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Integrase core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_01149</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HJ28"/>
    <dbReference type="OrthoDB" id="2686689at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058913">
        <property type="entry name" value="Integrase_dom_put"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46791:SF5">
        <property type="entry name" value="CLR5 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46791">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24764">
        <property type="entry name" value="rva_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase core" evidence="1">
        <location>
            <begin position="110"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF24764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8048719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="205" mass="24066" checksum="06BB3C69AC9208D8" modified="2023-09-13" version="1">MELLSQRNYDLPRKLLLRMQTRAVEHDIEYCLLYEITWHPIMSPPADDQLDEAVKAILGRHPNYGEKMVNGCLLSKNILIQRDRIRDAIRRVDPNGVSERRKQSLHRREYYVPGPNSLWHIDGNHKLIRIRSSGVHGGQSIETLVSRYLSIEILTNHRWMTHFNWMSQAIWGHWSIKLIKVLPYIVFGPCLWTVFCAKIVKSMTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HJU6</accession>
    <name>A0A9Q1HJU6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_02599</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HJU6"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8049724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="133" mass="15227" checksum="E03420234BE18280" modified="2023-09-13" version="1">MIVSYEVTFDSHYTKRTDSLTFQMVGWEQTGKSPYRCLQESLFPATTSQYTKRTGSLTFQMVGWQQTGKSPCRDLQESLLPATTTTITHSMTFQMVEWQQTGNSPCRRLHESQFPATTVSLYKSDNLLSKMGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HK45</accession>
    <name>A0A9Q1HK45_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_01187</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HK45"/>
    <dbReference type="OrthoDB" id="6152410at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47018">
        <property type="entry name" value="CXC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8048750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="169" mass="18219" checksum="4AB2A90350BF5820" modified="2023-09-13" version="1">MIHTKTRNRELVDRLYVLGLSISYDPVLAISTELGDKICHYYKMEKAVCPPELKGGLFTTATVDNIDHNPSSTSAHDLFHGTGISLFQHPDNEFSGASRAVAYTHRDAAGAKKNVSISPLHQCTSCCSSKARLTGAKARRSKLSRLSARTSSLTEGISLAEAPEIGCGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HK48</accession>
    <name>A0A9Q1HK48_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Glucose-6-phosphate exchanger SLC37A4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_00143</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Organophosphate:Pi antiporter (OPA) (TC 2.A.1.4) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8047998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HK48"/>
    <dbReference type="OrthoDB" id="3639251at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061513">
        <property type="term" value="F:glucose 6-phosphate:phosphate antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051337">
        <property type="entry name" value="OPA_Antiporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000849">
        <property type="entry name" value="Sugar_P_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43826">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE EXCHANGER SLC37A4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43826:SF3">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE EXCHANGER SLC37A4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002808">
        <property type="entry name" value="Hexose_phosphate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="76"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="133"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="224"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="300"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="330"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="365"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="392"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="1"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8047998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="429" mass="46730" checksum="54485C758672C15C" modified="2023-09-13" version="1">MNSYTLWRNINFCLMFISYATLFLCRKSFSYLIPAIIAEENLEKSQLGIISSSLVVAYCFSKFSCGILSDKVQPRIFLTVGLIAVGLLNIQFACSYSVPVFAFTWFLNGLAQGCGWPPCTKLLKQWYHPTELGTWWAILSSSSNLAGVLGPLLLSGAAYQYGWRPVMMATGSICILSSALAYTFIKNSPQEIGEKPLEINGKQTTKSENNDKKATMKDVLRSPFLYIISVIFACSTVQAGAYHDWAQLFLIQDKGFSSTVGSGFTSAFNIGGTIGCMASGYITDKLVAMSAQRRLGSPRFPYLVLTSLCCFFSQYYMLRFTTPETSKLPLLVSAFIIGYCSYGAISLNGVLVVENSPVHLSGTSHALGALAGNVGQVIAGFPLSYVSSLYDWSVSFSLVLAANILHVLLLLVSTRISLVIGAQPSKKTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HKJ3</accession>
    <name>A0A9Q1HKJ3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_01938</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HKJ3"/>
    <dbReference type="OrthoDB" id="3645095at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8049260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="171" mass="20285" checksum="F6AA3DD04BBAEC16" modified="2023-09-13" version="1">MQSLYIEANEPPLHIRWKTLSLQFALKLRSHRQNPTFETAFSHKFSDNGYIKSKWQELWDSFPENKLYQVKPTVGTVGNAAPRKRRDDLVLTRARIGHTYLTHAYLLHGEERPYCIPCDCDVTVSHILVDCHEYSHIRQKYYTVPDLKTFEHVDPFLILDFLKESDLYRKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HKL5</accession>
    <name>A0A9Q1HKL5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SCAN box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_02019</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HKL5"/>
    <dbReference type="OrthoDB" id="6077919at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF13">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCAN box" evidence="2">
        <location>
            <begin position="208"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="302"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="302"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="317"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="341"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8049320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="364" mass="42740" checksum="5CE37602CAEA210D" modified="2023-09-13" version="1">MPFNLDKFVASPSVEELDSLKKSEIVKVAKHYGIEFQPLMRKDEIKRYVLEYLVDESVLPSTVLETAEMEREREREEREMQKEKEEREMQREKEEREERERREEKAREHEFRLKQLELGVIKGSDPKIGLDTHVKFVPKFQEDNVEKFFNHFEKLGEQLKWPKDKWSILIQSNFTGKAQEVYSALSIEDSMDYDKVKKAILQAYELVPEAYRQKFRKYRKADTQTYVEFAYQKERHFDRWCASKKVSTFDTLRQLMLVEEFKRCVNDDIKTHLEENKADNLKEVANLADQYALTHKFGKVGQTQNKGQFSRPNKRYGSSSGTTSNSGSQSGSISPTNSGKKQPDSSRQKNDQVSSNKAAFKDIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HMH1</accession>
    <name>A0A9Q1HMH1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_04688</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8051208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HMH1"/>
    <dbReference type="OrthoDB" id="6361178at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8051208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="88" mass="9909" checksum="9AD8C1DB868FA0EB" modified="2023-09-13" version="1">MPHIERKNPIVFGGGQRSPGVTRGQNLKTLLTQYLEMRYGKRKNPIIFGGGQRSSGVTGGQTLKTLLTRYLKKESSYSSQTWYIDAPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0Y8I1</accession>
    <name>A0A9Q0Y8I1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">C-type mannose receptor 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_44914</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010001444">
        <property type="protein sequence ID" value="KAJ8017578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0Y8I1"/>
    <dbReference type="OrthoDB" id="6356110at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050111">
        <property type="entry name" value="C-type_lectin/snaclec_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22803">
        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0675">Receptor</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C-type lectin" evidence="1">
        <location>
            <begin position="46"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8017578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="212" mass="23005" checksum="C509E821D57BD4DB" modified="2023-09-13" version="1">MGGGGAWNDERCGMRNPSVCKMSKIIIEGTDVPVTPNGCDLGWVAYGGSCYGVPGKITAEWDRSQLSCQAVGAKLASIKDRYEQSVVTSVLAQEHGNPVWIGLNDLIVAGEYHWSDGTAVTYTNWDYLEPKENNGNCVAATTGVMAGLWQVKSCNTRYDYLCESVREGYTAAPTVTPPRNPTTPHDTNCAEGWIGYGDRCFGVSIIYIVNLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0Y9A8</accession>
    <name>A0A9Q0Y9A8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_43804</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000469">
        <property type="protein sequence ID" value="KAJ8018283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0Y9A8"/>
    <dbReference type="OrthoDB" id="6779087at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon gypsy-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF1">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="289"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="33"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="33"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8018283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="648" mass="72779" checksum="58A5DD7CB9009DD0" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YAL7</accession>
    <name>A0A9Q0YAL7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Tripartite motif-containing protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_43518</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000342">
        <property type="protein sequence ID" value="KAJ8018475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YAL7"/>
    <dbReference type="OrthoDB" id="252722at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047153">
        <property type="entry name" value="TRIM45/56/19-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25462">
        <property type="entry name" value="BONUS, ISOFORM C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25462:SF291">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Zinc finger C3HC4 RING-type" evidence="4">
        <location>
            <begin position="3"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8018475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="71" mass="7997" checksum="0E7E72D1F8409304" modified="2023-09-13" version="1">MRTFRNPKVLTCGHSFCEDPCLTRFVRPLNKISCPECRTETTLPPGGVKELLSNFTLIRAIRDEAGEETCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YBY5</accession>
    <name>A0A9Q0YBY5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_41607</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8019843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YBY5"/>
    <dbReference type="OrthoDB" id="7493297at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040390188" evidence="3">
        <location>
            <begin position="27"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="460"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="605"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="55"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="103"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="200"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="605"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="651"/>
            <end position="665"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8019843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="665" mass="72101" checksum="549AEC570C4930A8" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YCI0</accession>
    <name>A0A9Q0YCI0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">SCY1-like protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_42907</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000161">
        <property type="protein sequence ID" value="KAJ8018870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YCI0"/>
    <dbReference type="OrthoDB" id="79687at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14011">
        <property type="entry name" value="PK_SCY1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000179">
        <property type="entry name" value="SCY1 like pseudokinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051177">
        <property type="entry name" value="CIK-Related_Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12984:SF6">
        <property type="entry name" value="SCY1-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12984">
        <property type="entry name" value="SCY1-RELATED S/T PROTEIN KINASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="26"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8018870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="216" mass="24443" checksum="4B40D660DC34953C" modified="2023-09-13" version="1">MDMLNRLKSTVGGLIGNPVTREFDVGPHIASAGPGHLWRIYEGTKKTTKQKVSLFYLEKKYLDKFSRKDREKLLETLKIGPTKLTKLRHPNLLVVEHPLEESRESLAFATEPVFASLSNLLGCREGMPSPVPQVVTDHELYEVEIKYGLLQLTEALKFLHNNVKMIHGNICPENVILNANGAWKLAGFDFFIQSSNPPDQPVSKSKMHTVLLYIYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YCR1</accession>
    <name>A0A9Q0YCR1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_42380</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000063">
        <property type="protein sequence ID" value="KAJ8019194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YCR1"/>
    <dbReference type="OrthoDB" id="5983950at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8019194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="106" mass="11940" checksum="CC980E0C56BEB21F" modified="2023-09-13" version="1">MLFVINQARTLNKPTPVLTFDQPLWLKTYEIATSKALKVVLIPEGFRKLMNFLGNITFMMKDSGLKEAIGRLYGENTVDHIMTGKTVTKAPRAHYLTDATLSLKLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YE68</accession>
    <name>A0A9Q0YE68_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Solute carrier organic anion transporter family member</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_40370</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the organo anion transporter (TC 2.A.60) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YE68"/>
    <dbReference type="OrthoDB" id="5062115at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015347">
        <property type="term" value="F:sodium-independent organic anion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:monoatomic ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043252">
        <property type="term" value="P:sodium-independent organic anion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036058">
        <property type="entry name" value="Kazal_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004156">
        <property type="entry name" value="OATP"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00805">
        <property type="entry name" value="oat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11388">
        <property type="entry name" value="ORGANIC ANION TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11388:SF100">
        <property type="entry name" value="SOLUTE CARRIER ORGANIC ANION TRANSPORTER FAMILY MEMBER 4A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07648">
        <property type="entry name" value="Kazal_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03137">
        <property type="entry name" value="OATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100895">
        <property type="entry name" value="Kazal-type serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00282">
        <property type="entry name" value="KAZAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51465">
        <property type="entry name" value="KAZAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="78"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="112"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="143"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="201"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="242"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="285"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="359"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="394"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="432"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="555"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="645"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="78"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="domain" description="Kazal-like" evidence="11">
        <location>
            <begin position="478"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="18"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="28"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8020710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="686" mass="75143" checksum="265DCEA27806DBDB" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YEV1</accession>
    <name>A0A9Q0YEV1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">phosphatidate phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_39746</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 1,2-diacyl-sn-glycero-3-phosphate + H2O = a 1,2-diacyl-sn-glycerol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:27429"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="EC" id="3.1.3.4"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:27430"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the lipin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.4" evidence="4"/>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YEV1"/>
    <dbReference type="OrthoDB" id="4567at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008195">
        <property type="term" value="F:phosphatidate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032869">
        <property type="term" value="P:cellular response to insulin stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009062">
        <property type="term" value="P:fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019432">
        <property type="term" value="P:triglyceride biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026058">
        <property type="entry name" value="LIPIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031703">
        <property type="entry name" value="Lipin_mid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007651">
        <property type="entry name" value="Lipin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013209">
        <property type="entry name" value="LNS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031315">
        <property type="entry name" value="LNS2/PITP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12181">
        <property type="entry name" value="LIPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12181:SF12">
        <property type="entry name" value="PHOSPHATIDATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16876">
        <property type="entry name" value="Lipin_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04571">
        <property type="entry name" value="Lipin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08235">
        <property type="entry name" value="LNS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00775">
        <property type="entry name" value="LNS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LNS2/PITP" evidence="7">
        <location>
            <begin position="752"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="128"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="198"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="345"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="407"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="491"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="630"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="237"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="251"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="269"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="410"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="663"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="685"/>
            <end position="701"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8020216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="974" mass="109336" checksum="2AA9C82DEF03D59D" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YFI5</accession>
    <name>A0A9Q0YFI5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_41180</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8019544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YFI5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8019544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="64" mass="7277" checksum="AEACC3476336577C" modified="2023-09-13" version="1">MDQRKITTHLHTENLLTVEAIRIMSSNGTVPDMNPTGTTLRTGKNFKSCVNSHVHHNLYQRSDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YGA5</accession>
    <name>A0A9Q0YGA5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 17">Alkaline phosphatase</fullName>
            <ecNumber evidence="3 17">3.1.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">HOLleu_39243</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">Nanhai2018</strain>
            <tissue evidence="20">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a phosphate monoester + H2O = an alcohol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15017"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:67140"/>
            <dbReference type="EC" id="3.1.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="15">Binds 1 Mg(2+) ion.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="15">Binds 2 Zn(2+) ions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the alkaline phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.1" evidence="3 17"/>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YGA5"/>
    <dbReference type="OrthoDB" id="5818554at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004035">
        <property type="term" value="F:alkaline phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16012">
        <property type="entry name" value="ALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.720.10:FF:000008">
        <property type="entry name" value="Alkaline phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001952">
        <property type="entry name" value="Alkaline_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018299">
        <property type="entry name" value="Alkaline_phosphatase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11596">
        <property type="entry name" value="ALKALINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11596:SF5">
        <property type="entry name" value="ALKALINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00245">
        <property type="entry name" value="Alk_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00113">
        <property type="entry name" value="ALKPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00098">
        <property type="entry name" value="alkPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00123">
        <property type="entry name" value="ALKALINE_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8 17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10 15 17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="19" id="KW-0732">Signal</keyword>
    <keyword evidence="18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9 15 17" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040317826" description="Alkaline phosphatase" evidence="19">
        <location>
            <begin position="21"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="505"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphoserine intermediate" evidence="14">
        <location>
            <position position="106"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="327"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="332"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="336"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="373"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="374"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="457"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR601952-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR601952-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU003946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAJ8021910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="528" mass="58201" checksum="709BB0B83D4DE171" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YJL3</accession>
    <name>A0A9Q0YJL3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Synembryn-A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_35958</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cell cortex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the synembryn family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YJL3"/>
    <dbReference type="OrthoDB" id="5585685at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001965">
        <property type="term" value="F:G-protein alpha-subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008376">
        <property type="entry name" value="Chaperone_Ric-8_A/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019318">
        <property type="entry name" value="Gua_nucleotide_exch_fac_Ric8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12425">
        <property type="entry name" value="SYNEMBRYN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12425:SF5">
        <property type="entry name" value="SYNEMBRYN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10165">
        <property type="entry name" value="Ric8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01802">
        <property type="entry name" value="SYNEMBRYN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8023499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="579" mass="64503" checksum="C08A94E8963E2AFC" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YKB9</accession>
    <name>A0A9Q0YKB9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Synaptonemal complex protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_36789</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the XLR/SYCP3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8024143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YKB9"/>
    <dbReference type="OrthoDB" id="9621324at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000795">
        <property type="term" value="C:synaptonemal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007286">
        <property type="term" value="P:spermatid development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051443">
        <property type="entry name" value="XLR/SYCP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006888">
        <property type="entry name" value="XLR/SYCP3/FAM9_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19368">
        <property type="entry name" value="XLR/SCP3/FAM9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19368:SF15">
        <property type="entry name" value="XLR_SYCP3_FAM9 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04803">
        <property type="entry name" value="Cor1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="XLR/SYCP3/FAM9" evidence="4">
        <location>
            <begin position="84"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="127"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="14"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="24"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8024143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="228" mass="26755" checksum="1C9E350A1DAA0A57" modified="2023-09-13" version="1">MPRAAKKAKITPKTKAEAGTEKFDFDEEENAEEFGENQDPNSPVVLKSVKKRPYEEDEDDELPEPHIDGEMQSMLQSFGADMRKSMTGKRKRLESFTQQTLKAANGKLEKVWKLQQTERQRLKEEYNKQLNTVLEQWETDIKKMKEQEEKLQNMFKQQQRLMQQSCVAVSQRLKNVRQLFDQYNKTVNDLENCHKDQQGGVCGELRKEMSLLHKKILMDTVRLKVPFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YKI8</accession>
    <name>A0A9Q0YKI8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_36883</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8024218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YKI8"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8024218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="118" mass="13618" checksum="9B5A143BE2FB92B2" modified="2023-09-13" version="1">MTCYPYLYRPPSVLKARFPYLYPRPWLGTRTMTSWYLYLHSCSCIHTTQRRCQFPRILLHIYLWDGLGLGSDSGPLDSDSAWTRGRGVPDSELARNWGHWNRNWLGLGLGGLGYSPGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YM74</accession>
    <name>A0A9Q0YM74_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">unspecific monooxygenase</fullName>
            <ecNumber evidence="5">1.14.14.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HOLleu_38611</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Nanhai2018</strain>
            <tissue evidence="17">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 14">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.14.1" evidence="5"/>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YM74"/>
    <dbReference type="OrthoDB" id="1055148at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004508">
        <property type="term" value="F:steroid 17-alpha-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042446">
        <property type="term" value="P:hormone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042448">
        <property type="term" value="P:progesterone metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000238">
        <property type="entry name" value="Cytochrome P450 2A6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24289:SF21">
        <property type="entry name" value="CYTOCHROME P450 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24289">
        <property type="entry name" value="STEROID 17-ALPHA-HYDROXYLASE/17,20 LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 14 15" id="KW-0349">Heme</keyword>
    <keyword evidence="11 14 15" id="KW-0408">Iron</keyword>
    <keyword evidence="13 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 14 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0492">Microsome</keyword>
    <keyword evidence="12 15" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="10 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="14">
        <location>
            <position position="466"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ8021421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="521" mass="59451" checksum="E3D782D92B98F1CA" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YQ01</accession>
    <name>A0A9Q0YQ01_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="18">G-protein coupled receptor No18</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HOLleu_34156</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Nanhai2018</strain>
            <tissue evidence="18">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8026338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YQ01"/>
    <dbReference type="OrthoDB" id="5957871at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060170">
        <property type="term" value="C:ciliary membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00637">
        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22752">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22752:SF10">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0969">Cilium</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 18" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="34"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="89"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="119"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="152"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="193"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="237"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="372"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="445"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="478"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="520"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="557"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="602"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="736"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="776"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="17">
        <location>
            <begin position="46"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="17">
        <location>
            <begin position="457"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="276"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="341"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="341"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ8026338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="813" mass="91777" checksum="F8339FE5B605F5F4" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YS90</accession>
    <name>A0A9Q0YS90_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Complement factor H-related protein 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_31476</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Virion</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YS90"/>
    <dbReference type="OrthoDB" id="10051774at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051503">
        <property type="entry name" value="ComplSys_Reg/VirEntry_Med"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45785">
        <property type="entry name" value="COMPLEMENT FACTOR H-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45785:SF2">
        <property type="entry name" value="COMPLEMENT FACTOR H-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0732">Signal</keyword>
    <keyword evidence="2 5" id="KW-0768">Sushi</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040515723" evidence="6">
        <location>
            <begin position="31"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="7">
        <location>
            <begin position="30"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="7">
        <location>
            <begin position="154"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="7">
        <location>
            <begin position="217"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="7">
        <location>
            <begin position="350"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="32"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="187"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="219"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="248"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="387"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8026595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="441" mass="48833" checksum="04B984DFD8AC60A4" modified="2023-09-13" version="1">MTLEMRCFNAINCLWTVQILNFTTFIGCIAECNPPGTRDILITISPDRFIYNLYDVVNLVCPTGYQLVGPRRVFCNPEGSWHRDTTITSCRGSCSLDYLENEVEVTVKDEKLMSRSVHSGDVAYFSCDGIIIGNPVSTCYDQSWSPEIPSCLASSCGRPVSTNSVVLVFPDSPGSEYENGTDVKFSCPTGYDLQGDVESLCLYGVWDFTQEPTCQALPCTPLTQNDPSIIVSPNLTTYESGLEIRYSCPTSHVVNGTVFTFCELGNWKSPDTPECVSCNSSVQCALNSTRLDRDVLCTEEPHGNTDPPCYGHWSKFLSCKCRDPDYRLIHSNYVYWCSDQNGFDEMLYSLECRAPCSVGLNENVIATNSAGIETVIIQDGDYVQYSCPDGYMENGPATVACSDGTWMISQPFSCIGKLILRLHVSGSLFLLQKMVKCSHVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BB90</accession>
    <name>A0A9Q1BB90_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Leucine-rich repeat-containing protein 23</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_38787</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Flagellum axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BB90"/>
    <dbReference type="OrthoDB" id="271226at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001051">
        <property type="entry name" value="Leucine-rich repeat-containing 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050836">
        <property type="entry name" value="SDS22/Internalin_LRR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46652:SF8">
        <property type="entry name" value="LEUCINE RICH REPEAT CONTAINING 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46652">
        <property type="entry name" value="LEUCINE-RICH REPEAT AND IQ DOMAIN-CONTAINING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14580">
        <property type="entry name" value="LRR_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0969">Cilium</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0282">Flagellum</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8021546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="340" mass="38674" checksum="23BF0F92FDA45EBB" modified="2023-09-13" version="1">MSDFEEQEYEEDQEGVEEPDLDQEEGEDVEGVEQEEEEKIPEVPLTEEIIGENLSLLCKIGNGLAHAYVRLDVKEKEITDITILSSYIHLRYVDLSANMLKDISSLNSLTHLLTLRCDRNLLTSAKLDELPYLQNASFSNNRITSLEGVNHPLLESLNLSFNEITNITGLDPSKLGRLHTLELRGNKLTSTAGVTLPNLKNLFLGGNLVTTIEGLERLEHLTTLHLRDNQLEKLDGFSESMKNLQYINLRGNNVTNVKETAKLKCLPKLRALILSDNPISDEDDYRMEVLINLRRIERLDKDEFTEEERQEAEEIYEQRRAEELAAEGTEEVIHSDGEEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BBD1</accession>
    <name>A0A9Q1BBD1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Retrovirus-related Pol polyprotein from transposon TNT 1-94-like beta-barrel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_42393</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000065">
        <property type="protein sequence ID" value="KAJ8019188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BBD1"/>
    <dbReference type="OrthoDB" id="6287527at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054722">
        <property type="entry name" value="PolX-like_BBD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22936">
        <property type="entry name" value="Pol_BBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Retrovirus-related Pol polyprotein from transposon TNT 1-94-like beta-barrel" evidence="1">
        <location>
            <begin position="9"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF22936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8019188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="87" mass="9404" checksum="05E80407BF8B1BDA" modified="2023-09-13" version="1">MTHEVKGLLVDFGATTHVVNDQSKFIRFDDDFNPADHYIGLADSSRTNNVAMGKGDASITLVDSNGNAQKAILKDALHTPSFKLVLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BCD0</accession>
    <name>A0A9Q1BCD0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase</fullName>
            <ecNumber evidence="5">3.5.1.52</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">N-glycanase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Peptide:N-glycanase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HOLleu_41864</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Nanhai2018</strain>
            <tissue evidence="16">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Specifically deglycosylates the denatured form of N-linked glycoproteins in the cytoplasm and assists their proteasome-mediated degradation. Cleaves the beta-aspartyl-glucosamine (GlcNAc) of the glycan and the amide side chain of Asn, converting Asn to Asp. Prefers proteins containing high-mannose over those bearing complex type oligosaccharides. Can recognize misfolded proteins in the endoplasmic reticulum that are exported to the cytosol to be destroyed and deglycosylate them, while it has no activity toward native proteins. Deglycosylation is a prerequisite for subsequent proteasome-mediated degradation of some, but not all, misfolded glycoproteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of an N(4)-(acetyl-beta-D-glucosaminyl)asparagine residue in which the glucosamine residue may be further glycosylated, to yield a (substituted) N-acetyl-beta-D-glucosaminylamine and a peptide containing an aspartate residue.</text>
            <dbReference type="EC" id="3.5.1.52"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the transglutaminase-like superfamily. PNGase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.52" evidence="5"/>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8020029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BCD0"/>
    <dbReference type="OrthoDB" id="409136at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000224">
        <property type="term" value="F:peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006516">
        <property type="term" value="P:glycoprotein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.10:FF:000011">
        <property type="entry name" value="peptide-N(4)-(N-acetyl-beta- glucosaminyl)asparagine amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.1020:FF:000001">
        <property type="entry name" value="Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.620.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1020">
        <property type="entry name" value="Peptide N glycanase, PAW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038680">
        <property type="entry name" value="PAW_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006588">
        <property type="entry name" value="Peptide_N_glycanase_PAW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050883">
        <property type="entry name" value="PNGase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036339">
        <property type="entry name" value="PUB-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018997">
        <property type="entry name" value="PUB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002931">
        <property type="entry name" value="Transglutaminase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12143">
        <property type="entry name" value="PEPTIDE N-GLYCANASE PNGASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12143:SF19">
        <property type="entry name" value="PEPTIDE-N(4)-(N-ACETYL-BETA-GLUCOSAMINYL)ASPARAGINE AMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04721">
        <property type="entry name" value="PAW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09409">
        <property type="entry name" value="PUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01841">
        <property type="entry name" value="Transglut_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00613">
        <property type="entry name" value="PAW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00580">
        <property type="entry name" value="PUG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00460">
        <property type="entry name" value="TGc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143503">
        <property type="entry name" value="PUG domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51398">
        <property type="entry name" value="PAW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PAW" evidence="15">
        <location>
            <begin position="406"/>
            <end position="610"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00032901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ8020029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="610" mass="70598" checksum="F9880101DD20B3D9" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BCW2</accession>
    <name>A0A9Q1BCW2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Heterogeneous nuclear ribonucleoprotein H2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_38154</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8023076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BCW2"/>
    <dbReference type="OrthoDB" id="431068at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12503">
        <property type="entry name" value="RRM1_hnRNPH_GRSF1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12504">
        <property type="entry name" value="RRM2_hnRNPH_CRSF1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050666">
        <property type="entry name" value="ESRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13976">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01228">
        <property type="entry name" value="EGGSHELL"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="6"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="104"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="299"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="185"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8023076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="436" mass="45527" checksum="109D1B5C721D0F85" modified="2023-09-13" version="1">MGDEGNVIRVRGLPWSSTVEDVLKFFDGVNIEGGEDGIHFTFTNDGRPTGEAYIKLESEEDVTEALKKDNEHLGKRYIEVFQSKMSEMQWVTERAGANALKEGQCVRLRGLPFECLKSDIVEFFKGLKITKDDITLPQDQDGRSTGEAYVQFSSKEDAQKALERHKQEIGHRYIEIFPCSPDDITTVPKMRRGANRPGPYDRVGRGGGGYGPPRGMGRFKGGYGGYGDGGEFGTYGGFGGYGGYGGGGGARFGRGGGGRGGWGGGGRVGYGGYGGGSYGGGGGGGGAKAGSDYVSSTGHHIHMRGLPFSASEKDIREWFNGIATPRVISMGFDDKGRKNGEADVEFGSASDAQSAMSRHRDNMGSRYIELFLHSSGGGRGGGYDGDGYNGNDGYGGGSYGAAGYGGDGGYGGGGGSGGYGGYGGDWGGSGGYGDGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BDY6</accession>
    <name>A0A9Q1BDY6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_38934</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BDY6"/>
    <dbReference type="OrthoDB" id="5957838at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1310.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="130"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8021664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="450" mass="51293" checksum="AADDA12EC5DBFC90" modified="2023-09-13" version="1">MSAYSQDFTETPGTSAELGRRQVRQVYLITYSQADHDKVPTRLAFAEKVVESFRHAHIEVRQWACSLEKHQKQGHHFHMAVKLEGLHRWLAVKNYLSAQYGLICHFSSLHSSYYTAWKYVTKEDTEYIQSEGHPNLVNPADGRQARRARSSSHSERDSNEEQATKKRKKRLSAYEVSQIILTRGIKTRVELLAFANAQKLEGKTDLAEFIVNRGAKCFAETIQVAWEMANAQEVLERSQKTRIQLLEETAQKDCVDGCGGRWKVLAEDILRRNNIEKGGFCQAVYELLDKGRGKNRNVILIGPANCGKTFLLLPLTVIYEVFCNPATNTFAWVGVEKAEVIFLNDFRWSQQLIPWHDLLLLLEGGLVHFPAPKTHFARDLILTGDTPVFATSKHALVYIKAGAIDDVETEMMSVRWRQFILQSQIPLVEQQVISPCGHCFANFILLNMQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BE32</accession>
    <name>A0A9Q1BE32_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_39413</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8022038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BE32"/>
    <dbReference type="OrthoDB" id="1334205at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052990">
        <property type="entry name" value="Sulfoquinovosidase_GH31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46959">
        <property type="entry name" value="SULFOQUINOVOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46959:SF2">
        <property type="entry name" value="SULFOQUINOVOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8022038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="129" mass="14370" checksum="AA6911A48B6D469F" modified="2023-09-13" version="1">MADFGEYLPLVLFSPVVSSVKSFINLWTVLWTQMYREVVEGTRVSETLSFGCVLALGVLHNYSVMSCAGDLIVDFSTAEGLASVIPVTLPLDMFRYGMNHFEMGVYMSIYGAIRTEELFALLTLLPLLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BEE9</accession>
    <name>A0A9Q1BEE9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Sterile alpha motif domain-containing protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_39553</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8022144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BEE9"/>
    <dbReference type="OrthoDB" id="10049949at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31025">
        <property type="entry name" value="SI:CH211-196P9.1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31025:SF9">
        <property type="entry name" value="SI:DKEY-286J15.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="130"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="268"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="130"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="282"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8022144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="362" mass="41882" checksum="4323390E2A557243" modified="2023-09-13" version="1">MAFTCHVTCGDFKKVITFTNRDTVLAEICNAFNQPKNTKIRLQRYENMWNDWVDIHNICELENGMRLRQVLPEKSTNFQSPTLHTPGSDSTVILEDDTDDSSIFDSLESIPMFGASCGGSRNPCEDYISTQSRSSIASSLPSQTLSSPSTVPSDMEEVEISQDEDCSNQNETVHSGQWPKVFKIPKMPKKLSEKMRENVFPGERERRLIVNLVFMKMSEYVVYPTSEQYTEVASALVREYPVLGNENFSSAPYLYWKTKIMDKYRNERRHLPPEKRKKTAMPSGSSQAKKTCNSEDMESLKRHEKWLIEEAKKSKKDNRKVQLLMQKTFMSRRSDITENPQKLELLKQKYPFLFSTEQVCQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BEG7</accession>
    <name>A0A9Q1BEG7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Tenascin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_34777</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BEG7"/>
    <dbReference type="OrthoDB" id="126772at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00087">
        <property type="entry name" value="FReD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00104">
        <property type="entry name" value="KAZAL_FS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050373">
        <property type="entry name" value="Fibrinogen_C-term_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036058">
        <property type="entry name" value="Kazal_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040941">
        <property type="entry name" value="GGGWT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143">
        <property type="entry name" value="FIBRINOGEN/TENASCIN/ANGIOPOEITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00050">
        <property type="entry name" value="Kazal_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00186">
        <property type="entry name" value="FBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00280">
        <property type="entry name" value="KAZAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100895">
        <property type="entry name" value="Kazal-type serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51465">
        <property type="entry name" value="KAZAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="2" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040304039" evidence="4">
        <location>
            <begin position="20"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="domain" description="Kazal-like" evidence="6">
        <location>
            <begin position="143"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="5">
        <location>
            <begin position="322"/>
            <end position="543"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8024771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="544" mass="58339" checksum="BD6B85728903B614" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BEW8</accession>
    <name>A0A9Q1BEW8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_39492</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8022097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BEW8"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8022097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="64" mass="6858" checksum="D9935637BEE2066B" modified="2023-09-13" version="1">MLFMGKPLDIFLNLSFHVLPLVISAPKILISCTSLVSGPIAMAVVPFPDLHPDYGIPCHRAAAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BF12</accession>
    <name>A0A9Q1BF12_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Translation machinery-associated protein 16</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_38132</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TMA16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8023058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BF12"/>
    <dbReference type="OrthoDB" id="270284at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1440.170:FF:000001">
        <property type="entry name" value="Translation machinery-associated 16 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.170">
        <property type="entry name" value="Translation machinery-associated protein 16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021346">
        <property type="entry name" value="Tma16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038356">
        <property type="entry name" value="Tma16_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13349">
        <property type="entry name" value="TRANSLATION MACHINERY-ASSOCIATED PROTEIN 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13349:SF2">
        <property type="entry name" value="TRANSLATION MACHINERY-ASSOCIATED PROTEIN 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11176">
        <property type="entry name" value="Tma16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="161"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="177"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="213"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00034127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8023058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="231" mass="26842" checksum="1C71CF781B5489A4" modified="2023-09-13" version="1">MPKSTKHAKAKKVVHPNSRKAHKAGAKVVHKEKVKKVHYETTLKQSVLQEKLLWMKDHIDEAKTSYSKKDLCELAEKYLKRFDEELEQINIIHGVGLRRSKQHAARESAIHLTLKKEVGEYETCGLEVPDLCDGRNLKLFKAWEGEIRYLQNIKLRRVKRNMNHQDTQADGDKDGEWQDEDGDDEIIVELEEEDSDLQDEEDKEELDGRQDEGEHDDTGEEDEDVTKIENT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BFF5</accession>
    <name>A0A9Q1BFF5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Prefoldin subunit 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_37631</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the prefoldin subunit beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BFF5"/>
    <dbReference type="OrthoDB" id="29646at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23163">
        <property type="entry name" value="Prefoldin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.370:FF:000002">
        <property type="entry name" value="Prefoldin subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027235">
        <property type="entry name" value="PFD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002777">
        <property type="entry name" value="PFD_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13303">
        <property type="entry name" value="PREFOLDIN SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01920">
        <property type="entry name" value="Prefoldin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="120"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="16"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="87"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8022667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="147" mass="16202" checksum="9AB9BE3E877A2058" modified="2023-09-13" version="1">MSAAKTSKKSAKGPSQEKIIADFNQLRQENRNLMAKVSELEGDVNEHRLVIETLKDVDGDRKCCRLVGGILVERTVKDVLPALEHNKEQIEKLVETLGAQIQAKSAELAEYREKYNIKVKGEQPPATSEEEKSGGSSQGVLVAKDSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BFM6</accession>
    <name>A0A9Q1BFM6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_35819</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BFM6"/>
    <dbReference type="OrthoDB" id="5988713at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8023389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="191" mass="22127" checksum="918F43B24786B6F2" modified="2023-09-13" version="1">MKTFKSQSNLKHHMRSTGPCAAKRQLKDEVADRQPNTSTATTPDESSSHYADSQVETAVAGLLVLEDGAENNGGDDNALEEDPIAITEEVRREMRDMMCNIYENNWGAIRTHHRRHNRVQDIYNFRLNELDGERIGRLLLGVYSHQRVQFKPNISFGFMLRHSVNGELRYFHVSHNNHILFERPYYTSRHC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BG91</accession>
    <name>A0A9Q1BG91_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">adenosine deaminase</fullName>
            <ecNumber evidence="4">3.5.4.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_35985</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>adenosine + H2O + H(+) = inosine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:24408"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16335"/>
            <dbReference type="ChEBI" id="CHEBI:17596"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="3.5.4.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the metallo-dependent hydrolases superfamily. Adenosine and AMP deaminases family. ADGF subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.4" evidence="4"/>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BG91"/>
    <dbReference type="OrthoDB" id="7202371at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004000">
        <property type="term" value="F:adenosine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006154">
        <property type="term" value="P:adenosine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046103">
        <property type="term" value="P:inosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000017">
        <property type="entry name" value="Adenosine deaminase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001365">
        <property type="entry name" value="A_deaminase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006331">
        <property type="entry name" value="ADGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006330">
        <property type="entry name" value="Ado/ade_deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01431">
        <property type="entry name" value="adm_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11409">
        <property type="entry name" value="ADENOSINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11409:SF39">
        <property type="entry name" value="ADENOSINE DEAMINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00962">
        <property type="entry name" value="A_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="Adenosine deaminase" evidence="11">
        <location>
            <begin position="200"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8023522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="533" mass="61149" checksum="13DB473EDA74B3D2" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BGJ8</accession>
    <name>A0A9Q1BGJ8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_36368</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BGJ8"/>
    <dbReference type="OrthoDB" id="382863at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053347">
        <property type="entry name" value="Axonemal_MT_stabilizer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37404">
        <property type="entry name" value="HCG1796489"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37404:SF1">
        <property type="entry name" value="HCG1796489"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8023822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="322" mass="36881" checksum="E9F45FDAF2F48C55" modified="2023-09-13" version="1">MPREERFDLKKTSSGKGHDYRPGYYFPASDFKRTINNPLPPDLAKQDEIIKPFTTTTGETHDFKHHGGILGNDQHKKAPGLWNVHYNKDLREKLQNRPWRKPLTMGNQETEVQAQYKGAQTQPGVDFNNKLAGNPQPTSLQTHHQNMPAPVRENTPKYKPTLVRDDGVMQLLDPYVPTSHHIHKRFSRHELDGVPKKDAATYWRCEDYPQAWGHGTKHNPLPNSSVPRERGPMRDKMVFKTPVVEPVRWPEKFKRIPHDGIKSIQTTSYQTPSDPKMREIFSCPVDTPWVIPDAGPKQTFAVPNMYSTEYGTYASGKPVTTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BIC0</accession>
    <name>A0A9Q1BIC0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Tubby-related protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_31789</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TUB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BIC0"/>
    <dbReference type="OrthoDB" id="8775810at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.750.20">
        <property type="entry name" value="SOCS box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.90.10">
        <property type="entry name" value="Tubby Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056159">
        <property type="entry name" value="Beta-prop_IFT121_TULP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001496">
        <property type="entry name" value="SOCS_box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036036">
        <property type="entry name" value="SOCS_box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025659">
        <property type="entry name" value="Tubby-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000007">
        <property type="entry name" value="Tubby_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16517">
        <property type="entry name" value="TUBBY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16517:SF2">
        <property type="entry name" value="TUBBY-RELATED PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24797">
        <property type="entry name" value="Beta-prop_WDR35_TULP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07525">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01167">
        <property type="entry name" value="Tub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00253">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00969">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158235">
        <property type="entry name" value="SOCS box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54518">
        <property type="entry name" value="Tubby C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50225">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="SOCS box" evidence="7">
        <location>
            <begin position="361"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="228"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="498"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="612"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="806"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="890"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="233"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="839"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="890"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="933"/>
            <end position="948"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="952"/>
            <end position="966"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8026839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1069" mass="118504" checksum="1A902EC237329215" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BJ58</accession>
    <name>A0A9Q1BJ58_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_32675</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BJ58"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040147606" description="Secreted protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8027514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="85" mass="9927" checksum="569D74D4D660C7E0" modified="2023-09-13" version="1">MPDHSHKWAVVIASFCSSVIALQHKMKHLPCYTLTEKSVANISMEQFVCRLRTREVFTEQGRDFKLLKTFLLCMWNGLDIQETHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BJL5</accession>
    <name>A0A9Q1BJL5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Sodium/glucose cotransporter 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_29888</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8027821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BJL5"/>
    <dbReference type="OrthoDB" id="6132759at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005412">
        <property type="term" value="F:D-glucose:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1730.10:FF:000027">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="entry name" value="Sodium/glucose cotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018212">
        <property type="entry name" value="Na/solute_symporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00813">
        <property type="entry name" value="sss"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11819:SF196">
        <property type="entry name" value="SODIUM_GLUCOSE COTRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11819">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00457">
        <property type="entry name" value="NA_SOLUT_SYMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="13"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="51"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="87"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="131"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="159"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="193"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="255"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="294"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="362"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="409"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="434"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="466"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="508"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="634"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="556"/>
            <end position="586"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8027821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="658" mass="72312" checksum="E6CBAD9C1F657A0E" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BJZ8</accession>
    <name>A0A9Q1BJZ8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Solute carrier family 13 member 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_30200</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC13A/DASS transporter (TC 2.A.47) family. NADC subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BJZ8"/>
    <dbReference type="OrthoDB" id="6493944at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015556">
        <property type="term" value="F:C4-dicarboxylate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005310">
        <property type="term" value="F:dicarboxylic acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01115">
        <property type="entry name" value="SLC13_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001898">
        <property type="entry name" value="SLC13A/DASS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10283">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 13 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10283:SF82">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 13 MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00939">
        <property type="entry name" value="Na_sulph_symp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="18"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="41"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="86"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="124"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="284"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="347"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="376"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="444"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="475"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="567"/>
            <end position="590"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8028068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="628" mass="70018" checksum="D2532FE20C3716EB" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BLM8</accession>
    <name>A0A9Q1BLM8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Neuronal acetylcholine receptor subunit alpha-9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">HOLleu_28115</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">Nanhai2018</strain>
            <tissue evidence="19">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Synaptic cell membrane</location>
            <topology evidence="14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8028868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BLM8"/>
    <dbReference type="OrthoDB" id="5975154at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022848">
        <property type="term" value="F:acetylcholine-gated monoatomic cation-selective channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18997">
        <property type="entry name" value="LGIC_ECD_nAChR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19051">
        <property type="entry name" value="LGIC_TM_cation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000043">
        <property type="entry name" value="AcetylCholine Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000016">
        <property type="entry name" value="Nicotinic acetylcholine receptor subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002394">
        <property type="entry name" value="Nicotinic_acetylcholine_rcpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00860">
        <property type="entry name" value="LIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00254">
        <property type="entry name" value="NICOTINICR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="8 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 19" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0770">Synapse</keyword>
    <keyword evidence="4 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="205"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="238"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="270"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="424"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="17">
        <location>
            <begin position="1"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="18">
        <location>
            <begin position="211"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="345"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="348"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00034099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAJ8028868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="446" mass="50204" checksum="CB309BD06FAE2BE2" modified="2023-09-13" version="1">MNRLMDDYSSAVRPVPDYRDTVTVDLSLTLVELLDMDEVNQIMTTSLWMTQKWQDAALTWNPRDYANITSVRIPIEKLWRPDLVLYNNANDNLEGGIRMTKAVVSSDGSVEWNAPAILKSACEMNPLKFPFDVQTCVLKFGSWSFHGNQLNVVINEEEADTGVYESSGEWALVSMPARREVTYYPCCPEPYPVVSYDVILRRSPLFYVMNLLLPCILLSAMILLGFCLPPDAAERITLTITVLLSLTVFQLLVAETLPPTSDVVPFISQYYALIISLVGICTVLSVVTLAMHHRGDSIQLSQRFRHAMLFLARVMFMKCPSIETAAKKYREIEYSRDLALKGSHNPAFEDEDGEVETEPFANGSSSDEVKGHTKKHEEALTPIDAYLMRISSGLEFLAQRQVGKDDGEQTSREWMESASVIDRLMSIIFLLLGTFTTVLMICLAAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BMD6</accession>
    <name>A0A9Q1BMD6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_28610</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BMD6"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8029258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="215" mass="22548" checksum="C5565D461DC3A14D" modified="2023-09-13" version="1">MGNLHIIVNLLVGEFDLQSVLRSCIPAPPTGCNPDYVQTLLNTPVDPTIEIRNADGTECYCSTNNCNDQTLDSTPVTVAPAVLNCVSCVSLDFGDPFLDAEFRSLSCEDAVIDGLNVIQTACAATDNHCVTVSASMESAGLPPFSTVLRSCASSANQGCLDANLIQSTLEAVLGFAVTNIAGTACYCQGNFCNDAPVQPGTPGENICLVHHDVMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BQ19</accession>
    <name>A0A9Q1BQ19_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_27088</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BQ19"/>
    <dbReference type="OrthoDB" id="5971719at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00174">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056767">
        <property type="entry name" value="C2H2-Znf_KIN17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25095">
        <property type="entry name" value="C2H2-zf_KIN17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="4">
        <location>
            <begin position="67"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="31"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="43"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8030623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="232" mass="25592" checksum="68A8916B3A0AEF1B" modified="2023-09-13" version="1">MSSMQTSSHLVWTKGRGRGFRPAGTFNPARRPGERKPHEGMESSVQTQKSSENEETGASNKKLSEKDTVGKYIAKYSYKANQDSPLGEAELSLSVGQYVTLVEKHKDNDMWCKVINEGGETGFVPTKYLQAVEEKITTLPWLANKQIDEEKDNQGSGVKGPYKAYVSAYNKPEPKPAIDLKQYYCDVCEKQLNGPQPYKAHMMSKAHKLEVELAEEREQDIAAAAAAGGGAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BQB9</accession>
    <name>A0A9Q1BQB9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Bifunctional D-cysteine desulfhydrase/1-aminocyclopropane-1-carboxylate deaminase, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_27388</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ACC deaminase/D-cysteine desulfhydrase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BQB9"/>
    <dbReference type="OrthoDB" id="10266364at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019148">
        <property type="term" value="F:D-cysteine desulfhydrase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027278">
        <property type="entry name" value="ACCD_DCysDesulf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43780">
        <property type="entry name" value="1-AMINOCYCLOPROPANE-1-CARBOXYLATE DEAMINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43780:SF2">
        <property type="entry name" value="1-AMINOCYCLOPROPANE-1-CARBOXYLATE DEAMINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="4">
        <location>
            <begin position="53"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="4">
        <location>
            <begin position="420"/>
            <end position="683"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8030851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="717" mass="81012" checksum="82A284A2D4733C98" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BSB9</accession>
    <name>A0A9Q1BSB9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="26">IgGFc-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="26" type="ORF">HOLleu_25085</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="26">Nanhai2018</strain>
            <tissue evidence="26">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="19">Forms the apical lamina, a component of the extracellular matrix.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
            <location evidence="2">Extracellular space</location>
            <location evidence="2">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="20">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="26">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6114226at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005911">
        <property type="term" value="C:cell-cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019904">
        <property type="term" value="F:protein domain specific binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048646">
        <property type="term" value="P:anatomical structure formation involved in morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009952">
        <property type="term" value="P:anterior/posterior pattern specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009792">
        <property type="term" value="P:embryo development ending in birth or egg hatching"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048598">
        <property type="term" value="P:embryonic morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002064">
        <property type="term" value="P:epithelial cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060562">
        <property type="term" value="P:epithelial tube morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048592">
        <property type="term" value="P:eye morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042063">
        <property type="term" value="P:gliogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030097">
        <property type="term" value="P:hemopoiesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048871">
        <property type="term" value="P:multicellular organismal-level homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051093">
        <property type="term" value="P:negative regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="1050"/>
            <end position="1059"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="1088"/>
            <end position="1097"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="1126"/>
            <end position="1135"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="1164"/>
            <end position="1173"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="1202"/>
            <end position="1211"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="1240"/>
            <end position="1249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00055075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS51233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="EMBL" id="KAJ8031778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="4330" mass="477978" checksum="633CC50E5BCECCF6" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BSS6</accession>
    <name>A0A9Q1BSS6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">[F-actin]-monooxygenase MICAL3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_25419</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BSS6"/>
    <dbReference type="OrthoDB" id="20799at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050540">
        <property type="entry name" value="F-actin_Monoox_Mical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057494">
        <property type="entry name" value="Rossman_Mical"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23167:SF54">
        <property type="entry name" value="[F-ACTIN]-MONOOXYGENASE MICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23167">
        <property type="entry name" value="CALPONIN HOMOLOGY DOMAIN-CONTAINING PROTEIN DDB_G0272472-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25413">
        <property type="entry name" value="Rossman_Mical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding" evidence="4">
        <location>
            <begin position="95"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="[F-actin]-monooxygenase MICAL1-3-like Rossman" evidence="5">
        <location>
            <begin position="247"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="507"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="507"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="578"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="595"/>
            <end position="613"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF25413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8032018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="629" mass="71828" checksum="6CD56C6B031B80BB" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BUE1</accession>
    <name>A0A9Q1BUE1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase A2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_23154</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BUE1"/>
    <dbReference type="OrthoDB" id="8962223at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF13">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="110"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="125"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="149"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8033032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="410" mass="45522" checksum="1C42F1705B4A4204" modified="2023-09-13" version="1">MIRLKRPYYKPMNWCPKLIAKNLESIVRRMHKHTWSLLIKKKRHFDRWCASKKVSTFDTLRQLMLVEEFKRCVNDDIKTHLEENKADKLSEVANLADQYALTHKFGKVGQTQNKGQFSRPNKKYGNSTGTAGNSGSQSGSISPTNSGKKQPDASRQKNDQVSANKAAFKDIICGFCQKKGHTMANCFKLAKLKETETASNALANVENSSEVVVTQRPNSNDKIKEEYLPFVSEGFISLDGNSAHPPVKIKILRDTGATQSLLLDGVLPLSDSTSTGANVLIQGVECGFISVPLHKINLKSDLVSGSVIVGVRPTLPVKGVSLLLGNDLAGGKVVANAILTDKPCDYNNKRNFQIYSLPVLSLELCHKSSPWMIIINIHPPKVRIQFPKVEQVGNHVRMIILNIHSLKMRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BUQ3</accession>
    <name>A0A9Q1BUQ3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">E3 ubiquitin-protein ligase TRIM56</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_23252</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BUQ3"/>
    <dbReference type="OrthoDB" id="8936585at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047153">
        <property type="entry name" value="TRIM45/56/19-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25462">
        <property type="entry name" value="BONUS, ISOFORM C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25462:SF296">
        <property type="entry name" value="MEIOTIC P26, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="16"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="7">
        <location>
            <begin position="95"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="7">
        <location>
            <begin position="162"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="200"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8033112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="698" mass="78488" checksum="FFACBAB999296775" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BV55</accession>
    <name>A0A9Q1BV55_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Tyrosine-protein kinase</fullName>
            <ecNumber evidence="12">2.7.10.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HOLleu_24243</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Nanhai2018</strain>
            <tissue evidence="17">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 12">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the protein kinase superfamily. Tyr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.2" evidence="12"/>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BV55"/>
    <dbReference type="OrthoDB" id="28230at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004715">
        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd05068">
        <property type="entry name" value="PTKc_Frk_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11845">
        <property type="entry name" value="SH3_Src_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000318">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000053">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000044">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050198">
        <property type="entry name" value="Non-receptor_tyrosine_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24418">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 12 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="1 10" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="2 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 12" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="SH3" evidence="15">
        <location>
            <begin position="68"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="14">
        <location>
            <begin position="134"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="252"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="17"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="280"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00051245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ8033873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="519" mass="58769" checksum="E4D15279ACCD63DF" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BYB5</accession>
    <name>A0A9Q1BYB5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_21790</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BYB5"/>
    <dbReference type="OrthoDB" id="10053392at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012444">
        <property type="entry name" value="DUF1647"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31389">
        <property type="entry name" value="LD39211P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31389:SF4">
        <property type="entry name" value="LD39211P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07801">
        <property type="entry name" value="DUF1647"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="30"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8034794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="380" mass="43754" checksum="473BAEEBA8E92732" modified="2023-09-13" version="1">MRNTYFVSGIWRYVQDWQIRFPNMARWFKALQGMFALFIAFVTGYLLASKSVRTEPMNQQLNRHLDHPMINKSIPKPSNGTLGSSTTKPVTKKPLLNRASLNITLGPDRLNYLDVDERMSGIDELYNKLVIVTAISDNHFIESKGIIATAQEMMPSKKIIVYDLGMNSETKKHIKSYCNVEYRKFPFKKYPKHVSKLLVYAWKPMIINITLNEFGAIYWGDAGNRFRKSLKEALPYVQRNHGYMTAFHSFDPKAHPVVKHQYYFTHPGMFDYFGINRTQYYTEANASPHAAANRQLFINSTTVQTKIVQPLVQCALKFECISPGDAKYGPHRFDASALALIIYKNMKYEWTPEKNNGELMNAIVINSRDNGNMYKPLLCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BYT7</accession>
    <name>A0A9Q1BYT7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_22134</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BYT7"/>
    <dbReference type="OrthoDB" id="7323790at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8035049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="79" mass="9690" checksum="8661CB4EB2F15E4B" modified="2023-09-13" version="1">MFRSKRSRYKKSDAVKIPNLLHKGGERMITIYNALQWDNDEDVNKYDKVKKQFSRYFEPRKTVTYLRYQFFTRSQKEGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BZX3</accession>
    <name>A0A9Q1BZX3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_19486</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8035723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BZX3"/>
    <dbReference type="OrthoDB" id="276744at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01345">
        <property type="entry name" value="CERVTRCPTASE"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8035723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="162" mass="18990" checksum="5E0259B0E72A6904" modified="2023-09-13" version="1">MLLYKALIRPILEYGLPAWSPLYKREQEAIEAVQRRATKLIPGLSELPYEVRLERLNLFSLAHRRLRGDMFFIYKYLHGLITTDHLLFTRSTVSVTRGHLYKLDKPRFSSLRRQSFLTQQAINHWNALPELVVGSPSLDAFKRRLDRFWSNKPGSYSIDGDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C1F9</accession>
    <name>A0A9Q1C1F9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_20182</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C1F9"/>
    <dbReference type="OrthoDB" id="29523at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="187"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="295"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="198"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="295"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="310"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="329"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="352"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="397"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="446"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="478"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="543"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="559"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="590"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="645"/>
            <end position="658"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8036261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1121" mass="123136" checksum="FB9B5BF799D86380" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C1P6</accession>
    <name>A0A9Q1C1P6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Dynein intermediate chain 3, ciliary</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_17725</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynein intermediate chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C1P6"/>
    <dbReference type="OrthoDB" id="366230at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036157">
        <property type="term" value="C:outer dynein arm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045504">
        <property type="term" value="F:dynein heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045503">
        <property type="term" value="F:dynein light chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003341">
        <property type="term" value="P:cilium movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036158">
        <property type="term" value="P:outer dynein arm assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000371">
        <property type="entry name" value="dynein intermediate chain 2, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001723">
        <property type="entry name" value="Dynein intermediate chain 3, ciliary"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050687">
        <property type="entry name" value="Dynein_IC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442:SF7">
        <property type="entry name" value="DYNEIN AXONEMAL INTERMEDIATE CHAIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442">
        <property type="entry name" value="DYNEIN INTERMEDIATE CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0969">Cilium</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0243">Dynein</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="9" id="KW-0505">Motor protein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="513"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="528"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="556"/>
            <end position="597"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8037016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="597" mass="68755" checksum="2DAB800EC08DF314" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C1Z9</accession>
    <name>A0A9Q1C1Z9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Netrin-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_17914</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C1Z9"/>
    <dbReference type="OrthoDB" id="9972745at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005604">
        <property type="term" value="C:basement membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="P:animal organ morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016358">
        <property type="term" value="P:dendrite development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008045">
        <property type="term" value="P:motor neuron axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009888">
        <property type="term" value="P:tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="3"/>
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        <property type="entry name" value="NTR_netrin-1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Netrin 1"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Netrin-A, isoform B"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="EGF"/>
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    <dbReference type="InterPro" id="IPR050440">
        <property type="entry name" value="Laminin/Netrin_ECM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008211">
        <property type="entry name" value="Laminin_N"/>
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    <dbReference type="InterPro" id="IPR002049">
        <property type="entry name" value="LE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056863">
        <property type="entry name" value="LMN_ATRN_NET-like_EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001134">
        <property type="entry name" value="Netrin_domain"/>
    </dbReference>
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        <property type="entry name" value="Netrin_module_non-TIMP"/>
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        <property type="entry name" value="TIMP-like_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10574:SF365">
        <property type="entry name" value="NETRIN-A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10574">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00053">
        <property type="entry name" value="EGF_laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24973">
        <property type="entry name" value="EGF_LMN_ATRN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00055">
        <property type="entry name" value="Laminin_N"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="NTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00643">
        <property type="entry name" value="C345C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00180">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00136">
        <property type="entry name" value="LamNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="TIMP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="LAMININ_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 8" id="KW-0424">Laminin EGF-like domain</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040319430" evidence="9">
        <location>
            <begin position="27"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin N-terminal" evidence="12">
        <location>
            <begin position="49"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="10">
        <location>
            <begin position="280"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="10">
        <location>
            <begin position="399"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="domain" description="NTR" evidence="11">
        <location>
            <begin position="466"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="282"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="301"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="399"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="401"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="420"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="432"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50189"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8037166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="591" mass="66947" checksum="7109D4EB82BEBB5C" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C353</accession>
    <name>A0A9Q1C353_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_18635</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C353"/>
    <dbReference type="OrthoDB" id="1917519at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8037742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="121" mass="14234" checksum="227C1EFE1A999B43" modified="2023-09-13" version="1">MLRGRTLLFLAEVKGHSRSPEVKVPKPCNRDISRFVMLRGRTLLFLTEVKGHSRSPEVKVQKPCNLGISRFVMSRGRTLLFLAEVRGHSRSPEVEVRKPFFLFFFFSFFWLFKFWFKGHVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C3K5</accession>
    <name>A0A9Q1C3K5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein 180</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_18389</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C3K5"/>
    <dbReference type="OrthoDB" id="62987at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040035">
        <property type="entry name" value="TMEM180"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28658:SF1">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN CONTAINING 13B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28658">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 180"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13347">
        <property type="entry name" value="MFS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="83"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="113"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="156"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="180"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="287"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="384"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="431"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8037550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="469" mass="53640" checksum="2541847EE7BD09F4" modified="2023-09-13" version="1">MACKSVDRNAFFLSMTIFGSGLMSCVFRVYYVKLFLDHYNISQPWFQFAQVVYLVWNAVNDPLFAYLQDNSEIVCFRSRRHSILYESPLYALSFLLFWFPWGQYQSHHWLIGVHLMVSLCCYDTLLTFVLLSYCSLFAEISKDLDYRLTLTRYSKLSGILASCSVTVVEFVSNGLDDFQAFQMCCIVIAFLAWISMHFTGKYVRTNPSYRYEKVTAMNKDIPESDAKSGTFGQPGKQLKEILTGDFIAVVATNFLQILHKEYGSNFMVIFGDQLIPAGDISDVTRKLIYGSTAFLPTVAVLCLSSVVSQYGYCDMIMWTYVINIILAIGMFVIGQSHTWILVAFFIIDRCLSSYIFSFFNLPLSDIIDRDQEKYGRKTPLSSSVFGYNALFTKPAVSVAPMFVVAMLNSYGYESLQNGSLTDAEHECLRNVMFNISCFLPIILSVCRIYTWSKYRVRHSHTKVAKHIET</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C3T7</accession>
    <name>A0A9Q1C3T7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Asparagine--tRNA ligase, cytoplasmic</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_18840</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C3T7"/>
    <dbReference type="OrthoDB" id="1931232at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004816">
        <property type="term" value="F:asparagine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006421">
        <property type="term" value="P:asparaginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04323">
        <property type="entry name" value="AsnRS_cyto_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000151">
        <property type="entry name" value="Asparagine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1910.20">
        <property type="entry name" value="asparaginyl-tRNA synthetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048952">
        <property type="entry name" value="AsnRS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594:SF16">
        <property type="entry name" value="ASPARAGINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594">
        <property type="entry name" value="ASPARTYL/LYSYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20917">
        <property type="entry name" value="AsnRS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Asparagine--tRNA ligase N-terminal" evidence="8">
        <location>
            <begin position="29"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="OB" evidence="7">
        <location>
            <begin position="103"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="53"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8037900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="204" mass="22730" checksum="4F912610344F1FA3" modified="2023-09-13" version="1">MDVIYISDKGNDATGDGSEENPLKTLLQEWEEVSLKQRKKAAKAFYVELAKKSDREKLDTENAERRAKNLEEAKKIVFKEDQSLPKPKVIKIKDVSQSVGERVKVEGWIHRLRRQGKSLMFVILRDGTGYLQCLLADELCQSYDAVTLTTEATVAFFGVVKDVPEGKHAPGGFELSVDFWKLIGSSPAGGAVNFVNVVRTTIPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C4Q0</accession>
    <name>A0A9Q1C4Q0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Histone-lysine N-methyltransferase EZH2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_18794</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C4Q0"/>
    <dbReference type="OrthoDB" id="112668at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070877">
        <property type="term" value="C:microprocessor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070878">
        <property type="term" value="F:primary miRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031053">
        <property type="term" value="P:primary miRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19867">
        <property type="entry name" value="DSRM_DGCR8_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.590">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040375">
        <property type="entry name" value="DGCR8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13482:SF3">
        <property type="entry name" value="MICROPROCESSOR COMPLEX SUBUNIT DGCR8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13482">
        <property type="entry name" value="MICRORNA PROCESSOR COMPLEX SUBUNIT DGCR8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00035">
        <property type="entry name" value="dsrm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DRBM" evidence="2">
        <location>
            <begin position="57"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8037866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="155" mass="17589" checksum="BD8F7BB0444A28A7" modified="2023-09-13" version="1">MGRQKKVLEIKKSKRGGRQTSVVKPVVPLCDVEMPSTTGNNAVRKKECALNMKDKTPVAILHEYCQKVLPAHPKYVVEESNNAHHPFEAVMFIEGTKYGREQAQSKKVARLNAVMMVNVDHRIGIFAKRNIQEGEELFFDYRYGPTDALKYVGIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C715</accession>
    <name>A0A9Q1C715_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytochrome b-c1 complex subunit 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_14064</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the UQCRH/QCR6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8039911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C715"/>
    <dbReference type="OrthoDB" id="405848at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006122">
        <property type="term" value="P:mitochondrial electron transport, ubiquinol to cytochrome c"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.20:FF:000001">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.20">
        <property type="entry name" value="Ubiquinol-cytochrome C reductase hinge domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003422">
        <property type="entry name" value="Cyt_b-c1_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023184">
        <property type="entry name" value="Ubol_cytC_Rdtase_hinge_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036811">
        <property type="entry name" value="Ubol_cytC_Rdtase_hinge_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15336:SF0">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT 6, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15336">
        <property type="entry name" value="UBIQUINOL-CYTOCHROME C REDUCTASE COMPLEX 7.8 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02320">
        <property type="entry name" value="UCR_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000019">
        <property type="entry name" value="Bc1_11K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81531">
        <property type="entry name" value="Non-heme 11 kDa protein of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 10" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Ubiquinol-cytochrome C reductase hinge" evidence="13">
        <location>
            <begin position="25"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="9"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="34"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="48"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR000019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000019-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ8039911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="83" mass="9714" checksum="E212FAEBFF6B23B4" modified="2023-09-13" version="1">MVLRRVRQDEEEEEEEDEDEEDLVDPRDEVVEKCEEHCTAVKAELEACTDRVNSRPGTEETCEQELFDFLHCVDHCVSISEID</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C8K0</accession>
    <name>A0A9Q1C8K0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_15081</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C8K0"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8040706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="117" mass="13162" checksum="6DC2814ED0B01BDE" modified="2023-09-13" version="1">MPHIERKNPIVFGGGQRSSGVTRGQTPKTLLTRYLEMRYGKRKNPIVFDGGQRSSGVTGGRMLKTLLTRYLKMHYGKRKNPIVHGGGQRSSGVTGRQTLKTLLTRYLKLGSLYKFHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C8U5</accession>
    <name>A0A9Q1C8U5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNA 3'-terminal phosphate cyclase</fullName>
            <ecNumber evidence="2">6.5.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">RNA terminal phosphate cyclase domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_14395</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. Likely functions in some aspects of cellular RNA processing. Function plays an important role in regulating axon regeneration by inhibiting central nervous system (CNS) axon regeneration following optic nerve injury.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 3'-end 3'-phospho-ribonucleotide-RNA + ATP = a 3'-end 2',3'-cyclophospho-ribonucleotide-RNA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23976"/>
            <dbReference type="Rhea" id="RHEA-COMP:10463"/>
            <dbReference type="Rhea" id="RHEA-COMP:10464"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:83062"/>
            <dbReference type="ChEBI" id="CHEBI:83064"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.5.1.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.4" evidence="2"/>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C8U5"/>
    <dbReference type="OrthoDB" id="25029at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003963">
        <property type="term" value="F:RNA-3'-phosphate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00874">
        <property type="entry name" value="RNA_Cyclase_Class_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.20:FF:000002">
        <property type="entry name" value="RNA terminal phosphate cyclase-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.65.10.20">
        <property type="entry name" value="RNA 3'-terminal phosphate cyclase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.20">
        <property type="entry name" value="RNA 3'-terminal phosphate cyclase, insert domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00200">
        <property type="entry name" value="RTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013791">
        <property type="entry name" value="RNA3'-term_phos_cycl_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023797">
        <property type="entry name" value="RNA3'_phos_cyclase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037136">
        <property type="entry name" value="RNA3'_phos_cyclase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000228">
        <property type="entry name" value="RNA3'_term_phos_cyc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017770">
        <property type="entry name" value="RNA3'_term_phos_cyc_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020719">
        <property type="entry name" value="RNA3'_term_phos_cycl-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013792">
        <property type="entry name" value="RNA3'P_cycl/enolpyr_Trfase_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036553">
        <property type="entry name" value="RPTC_insert"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03399">
        <property type="entry name" value="RNA_3prim_cycl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11096">
        <property type="entry name" value="RNA 3' TERMINAL PHOSPHATE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11096:SF0">
        <property type="entry name" value="RNA 3'-TERMINAL PHOSPHATE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01137">
        <property type="entry name" value="RTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05189">
        <property type="entry name" value="RTC_insert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005378">
        <property type="entry name" value="RNA3'_term_phos_cycl_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55205">
        <property type="entry name" value="EPT/RTPC-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52913">
        <property type="entry name" value="RNA 3'-terminal phosphate cyclase, RPTC, insert domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01287">
        <property type="entry name" value="RTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNA 3'-terminal phosphate cyclase" evidence="11">
        <location>
            <begin position="15"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="RNA 3'-terminal phosphate cyclase insert" evidence="12">
        <location>
            <begin position="186"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-AMP-histidine intermediate" evidence="9">
        <location>
            <position position="322"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="296"/>
            <end position="300"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00045867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR005378-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR005378-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF05189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8040174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="364" mass="38566" checksum="68D0B6AC6B631BF7" modified="2023-09-13" version="1">MASARALVMIDGSVMEGGGQILRMAAAFSCLTSQPITVNKIRHGRSKPGLRPQHLTGFQMMADICNGEIKGDQVGSTEITLRPSSIQAGSYMADTGTAGSVALLLQVSLPCTLFAPGPITMTFKGGTNAEMAPQIDYVTLVMKPMLEKFGVNFNCDIIRRGYYPKGGGEIVVNTKPIHQLKAVELTDRGKVTSIRGTAFVAGVLPFHIAKRMAQTASDMIGRQYPDVDVQIVPKQERREDAVGSGSGIIIVAETSTKCLLAGSALGKKGVQAEEVGRQAAESLLQSLTHGGCVDEHLQDQLIIFMALASGVSRVLSGPLTMHTKTAIYIAEKLTKAQFKITPAGSDSGCEIIECTGIGLQNNSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C8X9</accession>
    <name>A0A9Q1C8X9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Mitogen-activated protein kinase kinase kinase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_14601</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C8X9"/>
    <dbReference type="OrthoDB" id="266718at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16494">
        <property type="entry name" value="RING-CH-C4HC3_ZSWM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000286">
        <property type="entry name" value="Mitogen-activated protein kinase kinase kinase 1 (Predicted)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050538">
        <property type="entry name" value="MAP_kinase_kinase_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016">
        <property type="entry name" value="MAP KINASE KINASE KINASE SSK2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016:SF56">
        <property type="entry name" value="MAPKK KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="12">
        <location>
            <begin position="186"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="11">
        <location>
            <begin position="288"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="531"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="24"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="247"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="367"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="24"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="67"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="86"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="131"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="560"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8040340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="799" mass="88966" checksum="78EB65F9CC2D00FB" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C9Z0</accession>
    <name>A0A9Q1C9Z0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">C-type lectin domain family 4 member G</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_15174</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C9Z0"/>
    <dbReference type="OrthoDB" id="6369810at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050111">
        <property type="entry name" value="C-type_lectin/snaclec_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22803">
        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C-type lectin" evidence="1">
        <location>
            <begin position="41"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8040776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="173" mass="19269" checksum="F28B76E85E23BF54" modified="2023-09-13" version="1">MSSNFISFSPPMICFVYAVVIRVLQRTVSPGCLDPADALYYDGSCYFKVFRQVPGKPILSAGEAEEMCNEKNGHLAIIKNEVENTIVSEAAGVKQAWIGAIRNPNNTDEFIWQDGSALTFYCWRQREPNNLLGNENCVKTNYVRLGFWNDTPCKDDKTVHYGVCEKPAQSGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CAL2</accession>
    <name>A0A9Q1CAL2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">MARVEL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_11920</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CAL2"/>
    <dbReference type="OrthoDB" id="10028364at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008253">
        <property type="entry name" value="Marvel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050578">
        <property type="entry name" value="MARVEL-CKLF_proteins"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22776:SF49">
        <property type="entry name" value="MARVEL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22776">
        <property type="entry name" value="MARVEL-CONTAINING POTENTIAL LIPID RAFT-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51225">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="51"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="77"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="111"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="143"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="MARVEL" evidence="8">
        <location>
            <begin position="47"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="183"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="18"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="194"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8041180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="218" mass="23925" checksum="D3527139DC49488D" modified="2023-09-13" version="1">MMAPDNSTQEVTIEVDETGSQGESPSQSTSVTETTVTATTLNLNLLWVKMLPGQLTCVESFLSLLAFISILSGRTTWIFALVAVFSLLWTLFLICLHLFQLYRMFDAVPWMLAELIGTGVFVFVWFIADMILLGQLSRANTGAAFMCGLFAFLCFCVSAYIGVTFYLEQVKKQGTPMPSVAVAAEKAAEKAERDAEDDDDSNSEDSLSEMPTVNPIPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CB97</accession>
    <name>A0A9Q1CB97_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="7">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_12390</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C19 family. USP10 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="7"/>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CB97"/>
    <dbReference type="OrthoDB" id="429671at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030330">
        <property type="term" value="P:DNA damage response, signal transduction by p53 class mediator"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02257">
        <property type="entry name" value="Peptidase_C19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000092">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF687">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="4 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="9">
        <location>
            <begin position="476"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="125"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="269"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="602"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="125"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="269"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="303"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="338"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="350"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="370"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="408"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="439"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="608"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="623"/>
            <end position="633"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8041540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="859" mass="94762" checksum="AA094C5A0FD63C5F" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CBA7</accession>
    <name>A0A9Q1CBA7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Low-density lipoprotein receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_12672</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CBA7"/>
    <dbReference type="OrthoDB" id="2019384at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0675">Receptor</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="80"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8041767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="184" mass="21047" checksum="7CC3EF2D1FC1ECD0" modified="2023-09-13" version="1">MKDVERLQIYLTEFQSHKTKYEMLQIKNLVTFVVFFAMVQSQPRKEVPYLRSYARDLPLTFPCDSLEAKRAEDSGNLFHCNAALCTDRKFVCDSIIDCPNFADENAENCRSDWNEYCAIITKPDNGKPFNATFCENGMCITECDVCDGRFLDCSDASDELFCTVSQRPDPGRCEQYDTGLPEGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CCP6</accession>
    <name>A0A9Q1CCP6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Phospholipid phosphatase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_09746</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PA-phosphatase related phosphoesterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8042872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CCP6"/>
    <dbReference type="OrthoDB" id="8907274at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008195">
        <property type="term" value="F:phosphatidate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046839">
        <property type="term" value="P:phospholipid dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03384">
        <property type="entry name" value="PAP2_wunen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043216">
        <property type="entry name" value="PAP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10165">
        <property type="entry name" value="LIPID PHOSPHATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10165:SF103">
        <property type="entry name" value="PHOSPHOLIPID PHOSPHATASE HOMOLOG 1.2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="68"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="226"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="259"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="7">
        <location>
            <begin position="126"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8042872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="310" mass="34826" checksum="8FB1364840E32E51" modified="2023-09-13" version="1">MKGAATWSSGSFLLHVAMYIHGTKVPLVLVPVLLFRLPGVELPVFHRGFFCNDDSLGYPYKDSTVPAIVMYAVGIGLTFILVIITELFIYLHRQRMDVDYLSPKDDNHCCGGFMVPPLIFQMGRTLCVFGFGVLTTMAVTDVMKNVVGRLRPHFFSVCQPVLLPENCTDGVQNFYITADVCTGPDEDKLLEARRSFPSGHSSVSVYCMVYLAIYLQSRWQSRSLMYIRPMTQLIALMLATYTCLSRVSDYKHHWSDVLGGAILGTFIAVCMAFAVARVLRRKPSGDESYQNELFMEMSSRNRSSRAEGIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CCY6</accession>
    <name>A0A9Q1CCY6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">receptor protein-tyrosine kinase</fullName>
            <ecNumber evidence="2">2.7.10.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_09468</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="2"/>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8042655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CCY6"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004714">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013151">
        <property type="entry name" value="Immunoglobulin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050122">
        <property type="entry name" value="RTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416:SF611">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE TRANSMEMBRANE RECEPTOR ROR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00047">
        <property type="entry name" value="ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040180698" description="receptor protein-tyrosine kinase" evidence="10">
        <location>
            <begin position="21"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="438"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="29"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="330"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="436"/>
            <end position="765"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00051243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8042655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="805" mass="92162" checksum="FC2A0BA467A66DDE" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CDN3</accession>
    <name>A0A9Q1CDN3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Testis-specific serine/threonine-protein kinase 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_09660</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8042803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CDN3"/>
    <dbReference type="OrthoDB" id="193931at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050321">
        <property type="term" value="F:tau-protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000944">
        <property type="entry name" value="Testis-specific serine/threonine-protein kinase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF84">
        <property type="entry name" value="TESTIS SPECIFIC SERINE KINASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="38"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="373"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="589"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="400"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="589"/>
            <end position="611"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8042803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="723" mass="82990" checksum="7B2328A3BE0F29AF" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CE03</accession>
    <name>A0A9Q1CE03_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">X-box-binding protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_10358</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CE03"/>
    <dbReference type="OrthoDB" id="20960at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14691">
        <property type="entry name" value="bZIP_XBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052470">
        <property type="entry name" value="ER_Stress-Reg_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46542">
        <property type="entry name" value="X-BOX BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46542:SF1">
        <property type="entry name" value="X-BOX BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="1" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="BZIP" evidence="9">
        <location>
            <begin position="78"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="38"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="205"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="362"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="89"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="362"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8043327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="413" mass="44812" checksum="D08D76E30323679C" modified="2023-09-13" version="1">MSSTKILIATVPDTQGKTYITSATSTNLRTMLPAKPTAVTIGSSNNGVRPRPSCLADLDPKPNPGPRKRQRLNHLSPDEKLMRRKMKNRVAAQTARDRKKALMERLEDKIYEFEVQQTKILDENSLLRARNQKLEAENRELRKRLGLDVDTDAESDKSFHVTVKEEAESPESAAGAGSVSFIADGNDVHMESDVLLGILGEINGQGPPIEGPESIDLTHEGGGDSISPSGTDSSAATTAVVGTTAADMESIKELIRFDHVYFKRPTEKVIAIKEETKDINKSADSPLPQNNVIVAPEESFTDLDQSSSLDFLLTLDSKDAIRLSNPFAASKSESSNLEDNFDCDISSLLTCEDLDMCDTLSDSGKSTGSSADSGVMSDEAPSSPDFVDPLGSPVLPNCDWEESFIDLFPSLDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CGD6</accession>
    <name>A0A9Q1CGD6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ADP-ribosylation factor-like protein 14</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_07707</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CGD6"/>
    <dbReference type="OrthoDB" id="2011769at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11711">
        <property type="entry name" value="ADP RIBOSYLATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="4">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="88"/>
            <end position="91"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8044837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="147" mass="16137" checksum="D182B2BB2C398493" modified="2023-09-13" version="1">MKEAVKAIPTCGFNVEELTTATGMKLSVIDVGGGRKLNRAMYCTGAEGLIFVVDSSDEERFSEARDELKGLLLAEATTWGIPFVVMANKQDIPESVDPSNLIEALGLREFNCNKWTVQGTSAATGEGLRESIETLCEMIKSFRKERR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CLI4</accession>
    <name>A0A9Q1CLI4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_06015</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CLI4"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051008">
        <property type="entry name" value="Telomere_Capping_Maintenance"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35518:SF2">
        <property type="entry name" value="MAINTENANCE OF TELOMERE CAPPING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35518">
        <property type="entry name" value="MAINTENANCE OF TELOMOERE CAPPING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="101"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8047110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="124" mass="14347" checksum="8F1ADFFC0557CC7E" modified="2023-09-13" version="1">MNSPETQRWRVVEDCQQDFYFACQHIEEKNEWTVSDVAGPYEVTNGYCPYMYKFSLPTNGYMKQKLLEAAEGKPVWINYGPWLPGYVEPEPNFSPTTESKAVKLGISFFMLFIVCLVQLCTSHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CMN0</accession>
    <name>A0A9Q1CMN0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Sperm microtubule inner protein 1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_06792</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CMN0"/>
    <dbReference type="OrthoDB" id="410807at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054323">
        <property type="entry name" value="SPMIP1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35826">
        <property type="entry name" value="PROTEIN ATP6V1FNB-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35826:SF1">
        <property type="entry name" value="PROTEIN ATP6V1FNB-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22589">
        <property type="entry name" value="SPMIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sperm microtubule inner protein 1 C-terminal" evidence="2">
        <location>
            <begin position="75"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="34"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8047725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="186" mass="21825" checksum="B80A6AA09E423C02" modified="2023-09-13" version="1">MPRELFDVQRQNFWTESIHKEAYTRLAWHEKYSKEFARDKINAQNAQSQRLQAKFPPIVHNIPAVLSLKAQKKDMKNKKADLEKKKSDELAALAGRDPNALLVEMRAVTPAVRKQLYKGFSKEGTGRYAYLQVRKLKKPEDKYDFPITSSWDYGWRLDDVVKEFHAPKFGRSRIVKDSFYRTNGIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CNF2</accession>
    <name>A0A9Q1CNF2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_01445</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CNF2"/>
    <dbReference type="OrthoDB" id="6617753at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="133"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="133"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8048932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="183" mass="20164" checksum="E00E8F5C76E63568" modified="2023-09-13" version="1">MREAPAESVHTGSTQKSRQQLPSTSSADSGTRSAVPPTPRSSASKKRRVKAERSELISLAKARLLQESSEDEYDSQAKTWANELRRVDPRQQLFAKKAINDVLFEGRCGTLTRNSVQINCTQNVFHTVSAASTPHSTYSTHSGASSPPQPSPVFQVNTQEMGSHFVEANPLAHYFQNFEPPTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CNZ3</accession>
    <name>A0A9Q1CNZ3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_01154</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CNZ3"/>
    <dbReference type="OrthoDB" id="5988050at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="614"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="614"/>
            <end position="624"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8048723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="642" mass="72050" checksum="9808B7D6A1DF0B55" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CPM6</accession>
    <name>A0A9Q1CPM6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_01206</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CPM6"/>
    <dbReference type="OrthoDB" id="6147018at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46791:SF13">
        <property type="entry name" value="CLR5 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46791">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8048760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="140" mass="16228" checksum="833E26F7FCCC60BB" modified="2023-09-13" version="1">MDLFREMLNRGDFTGDLLDKSLVQFCFMSLIQCELDTVTEMWNTHKIRPSRNAHVPSGRPVVMYYAPHLWGFTDKLHILDPNNVDACANQAEFRSTIPCDKDVYDLCMDIIIRDNINLPKDGYNAVDLFLHLKHEILTLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CRG5</accession>
    <name>A0A9Q1CRG5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_01774</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CRG5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8049159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="59" mass="6536" checksum="75F37B0094484B3D" modified="2023-09-13" version="1">MCFGSVAVWPSSGSGFKLWLIALRFVTPGRSARTTLCMVCHPRHAIQVFAGYLPLFRPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CRL1</accession>
    <name>A0A9Q1CRL1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_02655</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CRL1"/>
    <dbReference type="OrthoDB" id="10043409at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="121"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8049761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="173" mass="19187" checksum="E3352A7107C5A89A" modified="2023-09-13" version="1">MGFQRSFTGWIGAVTAIFGLAFIQLGIRVTDLVNANRAVGVPMWGGFFLILSGVAIVVEVMGRSKRKSYYSFWKFPIVSLVANLFTFIIVCIIISLLTWAMYDGYLNDTGTDDERLNGLSFYVTIIVMATLLLIVSLFGMFIDCCGAFFVVPTEDFRGAPPAVYDTRPAVIYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CTG5</accession>
    <name>A0A9Q1CTG5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">NACHT, LRR and PYD domains-containing protein 14</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_03932</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CTG5"/>
    <dbReference type="OrthoDB" id="427518at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312">
        <property type="entry name" value="NACHT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312:SF2">
        <property type="entry name" value="NUCLEOTIDE-BINDING OLIGOMERIZATION DOMAIN-CONTAINING PROTEIN 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040286544" evidence="2">
        <location>
            <begin position="22"/>
            <end position="1089"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="475"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="791"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="domain" description="NACHT" evidence="3">
        <location>
            <begin position="620"/>
            <end position="770"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8050648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1089" mass="125175" checksum="AC217E8E23E8D791" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1GXK0</accession>
    <name>A0A9Q1GXK0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Tubulin alpha chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HOLleu_32722</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Nanhai2018</strain>
            <tissue evidence="15">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="11">Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the tubulin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1GXK0"/>
    <dbReference type="OrthoDB" id="1662883at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02186">
        <property type="entry name" value="alpha_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.600:FF:000005">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.20:FF:000001">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000002">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.600">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002452">
        <property type="entry name" value="Alpha_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01162">
        <property type="entry name" value="ALPHATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8 11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 11" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="13">
        <location>
            <begin position="34"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="14">
        <location>
            <begin position="241"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="423"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="424"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ8027554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="446" mass="49764" checksum="1090DA66A6CB61EA" modified="2023-09-13" version="1">MGNACWELYCLEHGIQPDGQMPSDKTIGACDESFNTFFNETGSGKHVPRAVFVDLEPTVIGKLPTSFLDEIRTGTYHQLFHPEQLITGKEDAANNYARGHYTVGKQKVDIVLDRIRKLADQCTGLQGFLIFHSFGGGTGSGFTSLLMERLSVDYGKLSKLEFAIYPAPRISSAVVEPYNAILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIAQVVSSITASLRFDGSLNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLTVAEITNACFETANQMVKCDPHHGKYMACCLLFRGDVVPKDVNAAVATIKTKRSIQFVDWCPTGFKVGINYQPPATVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEVGMDSADAEEGYDEEDEEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H140</accession>
    <name>A0A9Q1H140_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Apoptosis regulator Bcl-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_27812</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Bcl-2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8031167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H140"/>
    <dbReference type="OrthoDB" id="6021377at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051400">
        <property type="term" value="F:BH domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097192">
        <property type="term" value="P:extrinsic apoptotic signaling pathway in absence of ligand"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008630">
        <property type="term" value="P:intrinsic apoptotic signaling pathway in response to DNA damage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001836">
        <property type="term" value="P:release of cytochrome c from mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06845">
        <property type="entry name" value="Bcl-2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.437.10">
        <property type="entry name" value="Blc2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036834">
        <property type="entry name" value="Bcl-2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046371">
        <property type="entry name" value="Bcl-2_BH1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026298">
        <property type="entry name" value="Bcl-2_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002475">
        <property type="entry name" value="Bcl2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11256">
        <property type="entry name" value="BCL-2 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11256:SF47">
        <property type="entry name" value="BCL-2-LIKE PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00452">
        <property type="entry name" value="Bcl-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01862">
        <property type="entry name" value="BCL2FAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00337">
        <property type="entry name" value="BCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56854">
        <property type="entry name" value="Bcl-2 inhibitors of programmed cell death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50062">
        <property type="entry name" value="BCL2_FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="153"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="Bcl-2 Bcl-2 homology region 1-3" evidence="8">
        <location>
            <begin position="37"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8031167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="179" mass="20751" checksum="4849FDD6CF72437B" modified="2023-09-13" version="1">MATTSHGKIARELGEDYVRYKLGLETDISPSVYAETLRRAGDQEEERYSLALPWMVSQLHYDPILDGERSVRVIFDAVFENEECSWGRIVMVYVFAARLAKYCQAQGYEKDTLEQFGRYAGEYVECHLKRWIEEQGGWQNNYHDTFKTLPRKMPSYIAIFTVLGLWLGGVMVLRRVCSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H359</accession>
    <name>A0A9Q1H359_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Beta-1,4-galactosyltransferase</fullName>
            <ecNumber evidence="11">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_26939</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyses the transfer of galactose onto proteins or lipids.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the glycosyltransferase 7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="11"/>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H359"/>
    <dbReference type="OrthoDB" id="10016069at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008489">
        <property type="term" value="F:UDP-galactose:glucosylceramide beta-1,4-galactosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003859">
        <property type="entry name" value="Galactosyl_T"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027791">
        <property type="entry name" value="Galactosyl_T_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027995">
        <property type="entry name" value="Galactosyl_T_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19300">
        <property type="entry name" value="BETA-1,4-GALACTOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19300:SF38">
        <property type="entry name" value="BETA-1,4-GALACTOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02709">
        <property type="entry name" value="Glyco_transf_7C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13733">
        <property type="entry name" value="Glyco_transf_7N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02050">
        <property type="entry name" value="B14GALTRFASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Galactosyltransferase N-terminal" evidence="13">
        <location>
            <begin position="136"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="Galactosyltransferase C-terminal" evidence="12">
        <location>
            <begin position="254"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU368121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF13733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ8030496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="378" mass="44417" checksum="B5B8ED4BA24C4578" modified="2023-09-13" version="1">MFLYFTHELTSRRLRHLLGIGVVTVLFFITNLFANENALTPAWFVRGKILNAKYYSTRRNWKCEELTQTDGEHAPNLTFISTEEVERKIIGENIQEVTSSLSVINSEGFDIFNMTSLRQTFNRSAINFSDDFAYLPGGHWKPRKFNDSSKVALLVPFRNREQVLGVFLRHMVPFLLRQRLEFTIFIIEQANNLKFNRGMLANVGFKESLRFEDWDCIILHDIDFLPRYNNNSYTCKNMPRHLMSGIEKRPKLLPSEFNGVTGFSREQFVMMNGAPNRYWGWGGEDHDLRTRAEVKGFNISRTQWPYGYYDHIPHASHKDSDTEPKRREYVKQAEIYLSQDGLSNLNYPKPEVYFHPLYVHIMVNISKLPRPKPRRNSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H379</accession>
    <name>A0A9Q1H379_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_28033</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8031333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H379"/>
    <dbReference type="OrthoDB" id="10618297at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040495543" description="Ig-like domain-containing protein" evidence="3">
        <location>
            <begin position="22"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="282"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="454"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8031333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="493" mass="55971" checksum="0AD77090597ED4DE" modified="2023-09-13" version="1">MISLLKLVLFFSTVLKWQGKAQNVVFELLDSEVNEVIFVGERDIVLNCSVRCVTNAVITIKRGESVMVTSDRNDGYLEYNIDEVLMGDNGEYTCTVSYVDVNTGLNVTEMETLNLNFKEERPQCFRNGTEGEPFQVGDLLLMSCYCLEDDACLWVRSIDGEGTATVVPVFETISYRGKKIQRIKEEYTIFTNINIRYDCIVGQKFCSIGPQVKSTEDTIVNKVPSDDELSRQDGECHLFQTASLSNLKLTTEKANYQHMEYMVTTNSNAEVTDRGNALLRSISFGTSGITVIFILTVVIMYIRWRRHKNKLGNKIEHQYFNPVDTNNKSCNETNTVKDTGTYYHHIQDPDGRQNDVCAKDVCPISKVNGAYEYKLPTTDETFQRGKMFEKMGLDIAIHTDPSVTLEKNKSQNEIALSVPPKDVALSRGTFELHVENDWAVYSFHLYIGEDEKKLNLKRNDEEVPEDGTSERKEMQPSVTSSEIYAEVDRSLAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H429</accession>
    <name>A0A9Q1H429_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_21549</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H429"/>
    <dbReference type="OrthoDB" id="10063284at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8034632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="81" mass="9402" checksum="CF22DE357F5257BA" modified="2023-09-13" version="1">MTRAVQGILLRGYRSEAGNWYQLLLLRTADCTDLNKWIQNKCYMSNDIINELASLMAREVTVFQLLAKIRQSRFFAITADE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H471</accession>
    <name>A0A9Q1H471_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_25720</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H471"/>
    <dbReference type="OrthoDB" id="10051210at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331:SF1">
        <property type="entry name" value="GAG-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8032248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="102" mass="11827" checksum="F7CCA99A965263C3" modified="2023-09-13" version="1">MGDIEAIFHQVRVSPQHRDALRFLWRDGNLSLQSETYRMAVHLFGGVWNPCWASFALKRTAEDHKDDFDPQAIQTVMENFYADDCLKSVASEEQAKKLVREL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H4D3</accession>
    <name>A0A9Q1H4D3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CUB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_25467</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H4D3"/>
    <dbReference type="OrthoDB" id="10037236at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24251:SF37">
        <property type="entry name" value="CUB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24251">
        <property type="entry name" value="OVOCHYMASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="121"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="5">
        <location>
            <begin position="142"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8032055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="322" mass="35900" checksum="4441F974FFC5E4D1" modified="2023-09-13" version="1">MQRGRIVTELRRNFNLGILDPQIDLWSSINLITQYKVTLNSVQSDDVTLSDRLNEFYARFDLDNNSEPSPAPCDDDQPPFVVSDHGARRSFYKLDEHKASGPDGITPRLLKMTYQCQNRGFLARGMSLLILANVCFYAGLTTGNSVISLYGTSGQFQSPGYPNRIDGTQNTWMITAPLHFRLTITFLDFELAETPLCTDELEVDKLNFHKEYQIHESNGKLGTYTGTQLHQQSITSRSNAVSIVLTACSSSYFYNRKGIRVSFSAAYATTSPYQTSKKEATTKALVRSPTEYNDTPSTANTSGQGLLFSTLEAALVAFVFLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H5V9</accession>
    <name>A0A9Q1H5V9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Heterogeneous nuclear ribonucleoproteins A1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_20699</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H5V9"/>
    <dbReference type="OrthoDB" id="1875751at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48033">
        <property type="entry name" value="RNA-BINDING (RRM/RBD/RNP MOTIFS) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48033:SF10">
        <property type="entry name" value="RNA-BINDING PROTEIN SQUID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="61"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="161"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="234"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="280"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="234"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="250"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="299"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8036657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="338" mass="36383" checksum="3EEF0D5DFE000CF1" modified="2023-09-13" version="1">MAIRNNGQTPGELPTHMLRHETPLTETEASRTGRMRMGLIICTRRTPHMSIAYQLESERLRKLFLGGLSFSTTEDDIRKTFQDYGTIVDCVVIRGQPSQRGEQGPSRGFGFVTFAKSTEVDDVLEARQNTKIKMNGREIDIKRAMSREDTAKDPQVNVQTKKIFIGGIGNLKSDHLKDYFSQYGEVEQVIIPTQTGDSSRIRGFAFVAFTNFDIVDKLVAIKNHTINGMDLEVKKATPPTKRGEQEGGDFRGGNRGGGGGYGSGGGGGYDGYRPSYDDGYGRGGFQDDYGNQGSNFGPMRGGGRNYGGGGGGGGGPYGGSYGSGGGGYSRSGGGGRRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H7F0</accession>
    <name>A0A9Q1H7F0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_19731</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8035909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H7F0"/>
    <dbReference type="OrthoDB" id="2019149at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030417">
        <property type="entry name" value="MS4A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23320:SF165">
        <property type="entry name" value="MARVEL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23320">
        <property type="entry name" value="MEMBRANE-SPANNING 4-DOMAINS SUBFAMILY A MS4A -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="162"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8035909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="211" mass="23333" checksum="D8CD477C290C94A1" modified="2023-09-13" version="1">MQPSNQQQQVMWGNHAYPVVTSGSLGTQNSSNRNLWGSSVRHSMGNIQIACGIIEFALGVTLLCLPLSRYFDFPNVAWGVWNGVFAVTTGFLGVYSKRSKGMVITYMIMSIIAAVLSAICCGFTAAATPTVAYYRRVSMHDLVIVSYMFLQIYGYYSANADYAIYITLSIIFALQMLFSILGASFTCGALTSKNNQQQIFRFDNKKKTDPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H8K7</accession>
    <name>A0A9Q1H8K7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Isochorismatase domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_20601</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the isochorismatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H8K7"/>
    <dbReference type="OrthoDB" id="269496at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="CDD" id="cd01012">
        <property type="entry name" value="YcaC_related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.850:FF:000001">
        <property type="entry name" value="Isochorismatase domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.850">
        <property type="entry name" value="Isochorismatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000868">
        <property type="entry name" value="Isochorismatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036380">
        <property type="entry name" value="Isochorismatase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050993">
        <property type="entry name" value="Isochorismatase_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14119">
        <property type="entry name" value="HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14119:SF17">
        <property type="entry name" value="ISOCHORISMATASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00857">
        <property type="entry name" value="Isochorismatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52499">
        <property type="entry name" value="Isochorismatase-like hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isochorismatase-like" evidence="3">
        <location>
            <begin position="17"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00040688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8036583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="200" mass="21987" checksum="FA380FE93F0D19FC" modified="2023-09-13" version="1">MSSRLHVSPGNLSPACTAFLLCDMQEKFRPVIKHFPDILEVADRLVKTSKILQIPLIVTEQYPKGLGSTVEELDTSHAKGVYAKTKFSMVISPVEEQLGRLPNLTAVVLFGVETHVCVEQTAIDLVTRGLDVHVVADATSSRSMMDRMFAFERLRQAGVIVTTSEAVMFQLVGDKENPKFKEVQALVKNLAPTSGLCSEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H8P8</accession>
    <name>A0A9Q1H8P8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Low affinity immunoglobulin epsilon Fc receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_17935</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H8P8"/>
    <dbReference type="OrthoDB" id="8935730at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050111">
        <property type="entry name" value="C-type_lectin/snaclec_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22803">
        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0675">Receptor</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040396686" evidence="1">
        <location>
            <begin position="25"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="2">
        <location>
            <begin position="38"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8037184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="188" mass="21938" checksum="3C18B744B215563A" modified="2023-09-13" version="1">MLLCNVNLFQIFTIIFFLIFSTEQTDVELFCPENFILFQNNCYYFSNRQLLFHQAEGVCQSYSRNPSHQCHLTSIHSEEENSFLDECAEHIWGSVQYWLGAMKGTDRTPGSFSWLDGSPIKFLTYFKPQGGRVACLLNYMKKRWDYTYCSRTEKYFTCKTAATRRHCEVIENSLLKLGNVQLSTPVLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H9E2</accession>
    <name>A0A9Q1H9E2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">WW domain-binding protein 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_17887</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H9E2"/>
    <dbReference type="OrthoDB" id="10067323at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019007">
        <property type="entry name" value="Wbp11/ELF5/Saf1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13361">
        <property type="entry name" value="WW DOMAIN-BINDING PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13361:SF1">
        <property type="entry name" value="WW DOMAIN-BINDING PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09429">
        <property type="entry name" value="Wbp11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Wbp11/ELF5/Saf1 N-terminal" evidence="4">
        <location>
            <begin position="12"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="200"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="23"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="242"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="257"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="279"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="319"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="383"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="400"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="417"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="498"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="579"/>
            <end position="601"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF09429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8037141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="630" mass="70186" checksum="D3C9C0E8A3893822" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H9P2</accession>
    <name>A0A9Q1H9P2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_11797</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H9P2"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8041082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="82" mass="9013" checksum="A3C9ABCABCF5FF0B" modified="2023-09-13" version="1">MPEGCLPKKTLYSELKAGKRHPGSLLKHFKDNLKAFLKACSIPVHIRSKPFTKGARGELLGRLSATGSSNWCLLTNKGVTNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H9Z9</accession>
    <name>A0A9Q1H9Z9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Integrase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_12255</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="8958038at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006259">
        <property type="term" value="P:DNA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008042">
        <property type="entry name" value="Retrotrans_Pao"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331:SF1">
        <property type="entry name" value="GAG-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05380">
        <property type="entry name" value="Peptidase_A17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase zinc-binding" evidence="1">
        <location>
            <begin position="1187"/>
            <end position="1233"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF17921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8041442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1337" mass="151765" checksum="04E8E4EF8D80A0EF" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HBY8</accession>
    <name>A0A9Q1HBY8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_13967</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8039838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HBY8"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="104"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8039838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="132" mass="14888" checksum="256CB8D5C1F85A1B" modified="2023-09-13" version="1">MGSPEVKKQNFPKIPKIVIWVTRGQKAKYPKNSQDCNLASLGSKINISQEFPRSSSGVIRGQKAKAKFPKNSQDCYMGSPEVKKQNIPRIPKIMIWVTRGQKVKFPKNSQDSHQESPEVKQQNISSIPKIVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HC46</accession>
    <name>A0A9Q1HC46_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_11099</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HC46"/>
    <dbReference type="OrthoDB" id="6156608at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053134">
        <property type="entry name" value="RNA-dir_DNA_polymerase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24559:SF444">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24559">
        <property type="entry name" value="TRANSPOSON TY3-I GAG-POL POLYPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8043827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="333" mass="37296" checksum="CC04F95910E4351E" modified="2023-09-13" version="1">MGCGIFSLNIAGKQIECEVWVAETEAEGTLGTDILGDCNCLIDVRTSDLTFRELMVADQEIEEYSCSRVKLADNAVVPSNSEALVKGVLEKKFQGSAIMEMLPEFPQKHSEMLIARAVVDASMTTIPIRVFNLSNTPKTLYKGTSLATCEPIEELYCQMISTEKPLHEVRETTQITDTYEGLHKNLQDLLERSSGNLDAREKDQVKQLLIKYQNTFATSSTDIGRTDLVRHSINTGSANPIKQPARRLPPHRREMEKAELTRMLEAKIIQPSSSPWASPTVLVTKKDGSTRFCVDYRRLNNVTQTARRTHIPSLGLMIVSMPWLELAGSLHWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HCB7</accession>
    <name>A0A9Q1HCB7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">Transcription elongation factor 1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_15218</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Transcription elongation factor implicated in the maintenance of proper chromatin structure in actively transcribed regions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the ELOF1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HCB7"/>
    <dbReference type="OrthoDB" id="445983at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008023">
        <property type="term" value="C:transcription elongation factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.190:FF:000002">
        <property type="entry name" value="Transcription elongation factor 1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007808">
        <property type="entry name" value="Elf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038567">
        <property type="entry name" value="T_Elf1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20934">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20934:SF0">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05129">
        <property type="entry name" value="Zn_ribbon_Elf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU364033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8040815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="83" mass="9542" checksum="35E733D58989B2A5" modified="2023-09-13" version="1">MGRRRSRRKPPPKKSMLGTLDTQFTCPFCNHEKSCDVKMDRTRNIGIINCRICLEDFQTPITYLSEPVDVYSDWVDACEAANA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HD00</accession>
    <name>A0A9Q1HD00_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Tyrosine-protein kinase receptor Tie-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_15181</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HD00"/>
    <dbReference type="OrthoDB" id="6426792at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00053">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.300.10">
        <property type="entry name" value="Tie2 ligand-binding domain superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056619">
        <property type="entry name" value="C8-3_MUC4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052108">
        <property type="entry name" value="MEGF/SIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24035">
        <property type="entry name" value="MULTIPLE EPIDERMAL GROWTH FACTOR-LIKE DOMAINS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24035:SF109">
        <property type="entry name" value="PROTEIN DRAPER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23263">
        <property type="entry name" value="C8-3_MUC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="405"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="425"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8040783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="444" mass="48924" checksum="C853EAC650645D9E" modified="2023-09-13" version="1">MAIQELKASFLSYQRGYNLLRQPGDFLELGTKTPTTISLAVMGLFILLAAHRKSYTSLEYLSGYLLLFFYQWRRYGEIDASESCFYYRPSETHETFTNIDFTPTFDPEDPSITPQMIEDVCGTNIFCAFDLRATGDPALAATTAFSLNMAMSVLEANEEAGIPCPPLANPANGIVVINDATVGGTATYSCNEGFSLTGNMVRVFVLPPLSCIIQRPSVGIKEDVSVFVYGLPGLQSTPDAVKLRRALKNPNELRVPPSKERTVGRGTLCEIPLPSAQRGGRFGVFACETIENGYVTNSVKAVITSVRKCLLPADGRVTRTVNYGEEVLLRVTLPNGPLSRLRWTKDGSWRPQWYRRPAVTLQSVTLNDEGVYEIFQRGRRNRGLQAIIILYVRACHKGFYGFNCNQPCTCQNGGLCDELTGECICPPGFSGSSCEEGKISVIQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HD88</accession>
    <name>A0A9Q1HD88_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Coagulation factor IX</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_12820</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HD88"/>
    <dbReference type="OrthoDB" id="8909918at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000120">
        <property type="entry name" value="Putative serine protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.15.10.10">
        <property type="entry name" value="Bactericidal permeability-increasing protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017943">
        <property type="entry name" value="Bactericidal_perm-incr_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017942">
        <property type="entry name" value="Lipid-bd_serum_glycop_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252">
        <property type="entry name" value="ACROSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252:SF7">
        <property type="entry name" value="HYALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01273">
        <property type="entry name" value="LBP_BPI_CETP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55394">
        <property type="entry name" value="Bactericidal permeability-increasing protein, BPI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0720">Serine protease</keyword>
    <keyword evidence="2 7" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0768">Sushi</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040153969" evidence="7">
        <location>
            <begin position="36"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="10">
        <location>
            <begin position="230"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="9">
        <location>
            <begin position="333"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="9">
        <location>
            <begin position="427"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="9">
        <location>
            <begin position="522"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="8">
        <location>
            <begin position="641"/>
            <end position="883"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8041894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="886" mass="98675" checksum="3FD0BBA27DDF5B73" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HEG9</accession>
    <name>A0A9Q1HEG9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_09894</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8042990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HEG9"/>
    <dbReference type="OrthoDB" id="6142091at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8042990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="117" mass="13854" checksum="4D1F56FCE7B0E85B" modified="2023-09-13" version="1">MPFNLDEFVASPSVEELDSLKKSEIVKVAKHYGIEFQPLMRKDEIKRYVLEYLVDESILPSTVLETAITVPTDNTFELKRLEMEMNKEIRLKEMEREMQREKKKEKCKGRQENMNLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HEM8</accession>
    <name>A0A9Q1HEM8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_05586</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HEM8"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8046797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="93" mass="10546" checksum="32210BCD143B68A6" modified="2023-09-13" version="1">MLVLGGLRARSVWVVWLFPKHRFGFQVALVQCFIGLSSPVCFHPDDWVVRTSKTRSMPNFYCVGMLPNGEYAYMATCAGPRFVMNTSLRGVRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HGP5</accession>
    <name>A0A9Q1HGP5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">RNA-binding protein 25</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_07069</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HGP5"/>
    <dbReference type="OrthoDB" id="6275295at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000381">
        <property type="term" value="P:regulation of alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12446">
        <property type="entry name" value="RRM_RBM25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052768">
        <property type="entry name" value="RBM25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034268">
        <property type="entry name" value="RBM25_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18806">
        <property type="entry name" value="RBM25 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18806:SF4">
        <property type="entry name" value="RNA-BINDING PROTEIN 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="89"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="63"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="75"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8044341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="182" mass="19411" checksum="32421C3BB0DCD109" modified="2023-09-13" version="1">MSFPGRPFGLPGFAAAVPGATIAGGGPFNPHMPGGVFVPATQVGLIPAPPGVIRTTAPIQIRSTPPVHISSKPPENNEEKKDGPEAPKTTVFVGNISEKAPDVLVRQLLAKCGVVLNWKRVQGASGNLQAFGFCEYKEPEHTLRAIRLLHDLTLGDKNLLVSISREVKSNIFRCYPASQTKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HI50</accession>
    <name>A0A9Q1HI50_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_05315</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HI50"/>
    <dbReference type="OrthoDB" id="5982640at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8046590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="230" mass="26595" checksum="E927465DA8F2B074" modified="2023-09-13" version="1">MKQISKNGFFQGDNSKDFIVALNDFSFEKNKYEGTFVRLEKCPPHIYGVVINEDGQSEEEFWKELKRLDLKLVNVGFTQQNTIKGCGNRMEVVEKQITNELDLACSTIFCLIVDFTTTTPFRGVEDDIRGKVGLYLDKDFAKRLQLPIPTKWVLTSQEMITYLTNKIQEKKTQCINNNTMYTTKDMESCDLQSVKMLVQQLRLPRVELKEDTIFYANEVDALAERLNSTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HKA7</accession>
    <name>A0A9Q1HKA7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Tyrosine-protein kinase receptor Tie-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_03551</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HKA7"/>
    <dbReference type="OrthoDB" id="441660at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.300.10">
        <property type="entry name" value="Tie2 ligand-binding domain superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0675">Receptor</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040427329" evidence="1">
        <location>
            <begin position="25"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="317"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS00022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8050369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="367" mass="39827" checksum="4E97802EA0B3EF14" modified="2023-09-13" version="1">MMDGLVKVLFLGFISFSNVALIAGTGEQPDQGFFLASSPPAVGRKERNAYIYAIQTDISFNDGDIEIYHENGYPKYVIDSGDILGDGIRWGCYVNVGVPSRYPGSRFGQYNGKLTPFDFPSSPIFTGHTFLKQSSKILNTGGVYTVTVYPDYVGATSLSEETSIGVGFTPGCPRIKWCKGKQRNFHTGPRLTLSTIEDGGLYTIQRPKRGGRGFFSQIEVLVASCPEGMYDNSGTCMSGATCMNGGVLRDKSNDCICPPYYGTFNCRCVVEPRENGDQFVLGDTRTPDNNELTCSDLPGGNTKCRGVLICYGDLYGCKCAPGWRGDACDRPCPVGKWGVECSLTCPQSEPKCSPYFGPSANLRPTCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HKT7</accession>
    <name>A0A9Q1HKT7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">protein adenylyltransferase</fullName>
            <ecNumber evidence="12">2.7.7.108</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">HOLleu_01385</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="22">Nanhai2018</strain>
            <tissue evidence="22">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = 3-O-(5'-adenylyl)-L-threonyl-[protein] + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:54292"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:13847"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:138113"/>
            <dbReference type="EC" id="2.7.7.108"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-tyrosyl-[protein] + ATP = O-(5'-adenylyl)-L-tyrosyl-[protein] + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:54288"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:13846"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:83624"/>
            <dbReference type="EC" id="2.7.7.108"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the fic family.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.108" evidence="12"/>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HKT7"/>
    <dbReference type="OrthoDB" id="439046at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070733">
        <property type="term" value="F:AMPylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3290.10">
        <property type="entry name" value="Fido-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003812">
        <property type="entry name" value="Fido"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036597">
        <property type="entry name" value="Fido-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040198">
        <property type="entry name" value="Fido_containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13504">
        <property type="entry name" value="FIDO DOMAIN-CONTAINING PROTEIN DDB_G0283145"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13504:SF34">
        <property type="entry name" value="PROTEIN ADENYLYLTRANSFERASE FICD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02661">
        <property type="entry name" value="Fic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140931">
        <property type="entry name" value="Fic-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51459">
        <property type="entry name" value="FIDO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 19" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="3 22" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="16"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="19">
        <location>
            <begin position="95"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="Fido" evidence="21">
        <location>
            <begin position="273"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="active site" evidence="15">
        <location>
            <position position="351"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="355"/>
            <end position="362"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="387"/>
            <end position="388"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="395"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for autoinhibition of adenylyltransferase activity" evidence="17">
        <location>
            <position position="222"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="18">
        <location>
            <position position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR640198-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR640198-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR640198-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR640198-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KAJ8048886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="442" mass="49502" checksum="6D6F7AF5D2454C26" modified="2023-09-13" version="1">MCKTTEIQITINKSSLICFLLGGVITAVLFSAIHLNFSLTLDRRWAALRQHVTERALLVPGMGLTPTVPTSNPLTRWALSMPVYSSDTRVLKGEALAALHQAIDAKKNGKKVKALKLFQHALALDPNHADVLNEYGEFLEDEDVVKADHLYTCAVIFNGSHSKALSNLRRTSASVAEIDKQMLDSIDAKHEQFLKISESSRVFERARQEFYYLQVYHTTAIEGNTLTLEQMRSIMETGLAIGGKSILEHNEVLGLELALQYINSTLMQRIGQITVKDILAIHHRVLGHVNPIDAGHVRTSQVFIGGHVPPPSTEIEDLMDEFVEWLNSEEALLLHPVEFAALAHYKLVYIHPFLDGNGRTARLLMNLILMRAGFPPVVVKLSERHTYYETIKMGNLGDLRPFIRFISHSLEDMLDAYIYLDSDPDHSITDVKESEVHVIGPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HLS7</accession>
    <name>A0A9Q1HLS7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Zinc finger MYM-type protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_03205</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HLS7"/>
    <dbReference type="OrthoDB" id="10063284at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025398">
        <property type="entry name" value="DUF4371"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008906">
        <property type="entry name" value="HATC_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45749">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45749:SF21">
        <property type="entry name" value="DUF4371 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05699">
        <property type="entry name" value="Dimer_Tnp_hAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14291">
        <property type="entry name" value="DUF4371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4371" evidence="3">
        <location>
            <begin position="161"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="HAT C-terminal dimerisation" evidence="2">
        <location>
            <begin position="684"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8050128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="764" mass="87065" checksum="FD977C6F9BE7DAD7" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YBM5</accession>
    <name>A0A9Q0YBM5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_41505</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8019778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YBM5"/>
    <dbReference type="OrthoDB" id="8411361at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8019778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="64" mass="7533" checksum="B437D6F218E92D40" modified="2023-09-13" version="1">MKAIDVCYKAMFVFNLEYQKACIGTWEFLQKIVYEHQGNVKCNQIREFRTFCACAIDKDNVTKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YCN3</accession>
    <name>A0A9Q0YCN3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_39882</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YCN3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8020317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="87" mass="10144" checksum="156CF1DC4A9AA149" modified="2023-09-13" version="1">MRKLSPPCTYTNRLTNMAACYSLVCKSLDLLKWLSIDKLCEKLVIFSKECKLRSNSSNRMEDCDIVLPFILIFCLKCCRFIVKLGKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YD61</accession>
    <name>A0A9Q0YD61_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="19">DNA-binding protein SMUBP-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">HOLleu_42284</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">Nanhai2018</strain>
            <tissue evidence="19">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DNA2/NAM7 helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000049">
        <property type="protein sequence ID" value="KAJ8019245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YD61"/>
    <dbReference type="OrthoDB" id="6513042at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18044">
        <property type="entry name" value="DEXXQc_SMUBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001146">
        <property type="entry name" value="DNA-binding protein SMUBP-2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.50:FF:000002">
        <property type="entry name" value="Immunoglobulin mu DNA-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1110.10">
        <property type="entry name" value="AN1-like Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.50">
        <property type="entry name" value="R3H-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035896">
        <property type="entry name" value="AN1-like_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050534">
        <property type="entry name" value="Coronavir_polyprotein_1ab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001374">
        <property type="entry name" value="R3H_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036867">
        <property type="entry name" value="R3H_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004483">
        <property type="entry name" value="SMUBP-2/Hcs1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048761">
        <property type="entry name" value="SMUBP-2_HCS1_1B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000058">
        <property type="entry name" value="Znf_AN1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00376">
        <property type="entry name" value="IGHMBP2 family helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43788:SF8">
        <property type="entry name" value="DNA-BINDING PROTEIN SMUBP-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43788">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01424">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21138">
        <property type="entry name" value="SMUBP-2_HCS1_1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01428">
        <property type="entry name" value="zf-AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00393">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00154">
        <property type="entry name" value="ZnF_AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118310">
        <property type="entry name" value="AN1-like Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82708">
        <property type="entry name" value="R3H domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51061">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51039">
        <property type="entry name" value="ZF_AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="19" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0347">Helicase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="R3H" evidence="18">
        <location>
            <begin position="714"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="domain" description="AN1-type" evidence="17">
        <location>
            <begin position="950"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="667"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="821"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="858"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1000"/>
            <end position="1046"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="821"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="834"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="858"/>
            <end position="875"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAJ8019245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1046" mass="117464" checksum="495849C2FFA17401" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YEG7</accession>
    <name>A0A9Q0YEG7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ER membrane protein complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_39808</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the ER membrane protein complex (EMC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Endoplasmic reticulum membrane</location>
            <topology evidence="5">Peripheral membrane protein</topology>
            <orientation evidence="5">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the EMC2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YEG7"/>
    <dbReference type="OrthoDB" id="124397at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072546">
        <property type="term" value="C:EMC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000478">
        <property type="entry name" value="GG16802"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039856">
        <property type="entry name" value="EMC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055217">
        <property type="entry name" value="TPR_EMC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12760">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22890">
        <property type="entry name" value="TPR_EMC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 4" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="EMC2 TPR-like" evidence="6">
        <location>
            <begin position="68"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="4">
        <location>
            <begin position="82"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="4">
        <location>
            <begin position="150"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU367091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF22890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8020259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="286" mass="32788" checksum="6F1B55080427940E" modified="2023-09-13" version="1">MAATMNFEDVRDKLRKWREDKVRNSVETADLGEYLLANYRRKLGDEVWPLYEQVSIAALDCGRLSLARLCIHAVRQQFPHSSRSKRLHGMLEEAEGEFDKARETYNDILKDDPANGLVMKRLIAIHKGQNDIPGAIKELNKYIENFMSDSEAWMELAELYIQEQHYSKAAFCFEELILSNPHHHLYHQRYAEIMYTQGGPENLEIARKYFAQAVKLNSNNMRALYGMFLSASHLAASSKATIKKKTENTKYASWAAAQIAHKYKQAEPQVEVVGEMLDKLAITSGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YGH2</accession>
    <name>A0A9Q0YGH2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Melatonin receptor type 1B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_39628</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8022213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YGH2"/>
    <dbReference type="OrthoDB" id="6117944at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00637">
        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24228">
        <property type="entry name" value="B2 BRADYKININ RECEPTOR/ANGIOTENSIN II RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24228:SF72">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="33"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="69"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="105"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="149"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="195"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="267"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="297"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="10">
        <location>
            <begin position="49"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8022213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="350" mass="38853" checksum="3824963BE7AD8704" modified="2023-09-13" version="1">MEAGFNDTFSDEGLSPSTPWSKEFVFESSNQRLIAAIVTCLVTVIGIPGNSMVILAVMLSKKLQNRTNVFVVNLACADMLTCSVLPFQAVALLSETWPLSTELCGAVAILMWLGLGSAVMNLALIAFTRYSIITKSRAFTDRMFTNNRICLLVCGAWLIPPVFILIPPAFGVGAVAYSHRYKLCTADSTNPMSDIYAYISLLVELPCLVVIIVCYFNIYRFVRKAGFNLTQSKRFTIANGLQNWGKTPQISTEVAVFHRQVKVTKKLFIIVCTYIICVMPFGLACAIPAPIYPIIPWAYLLLFLNLCLNPIIYGIKHPQFKEVFWPIITCSWKNIPEPSSLIASFSNIAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YGH5</accession>
    <name>A0A9Q0YGH5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_38809</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YGH5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8021564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="55" mass="6295" checksum="958EA62078C83F53" modified="2023-09-13" version="1">MVNSCHPKLTDKIWVILLETSTQKLATLQKIVPVNVQREGTNIYVRDKGKHPPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YHL1</accession>
    <name>A0A9Q0YHL1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_37114</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YHL1"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8022269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="62" mass="7185" checksum="D26EEADA0D3F801B" modified="2023-09-13" version="1">MCKPIKKFRPLRALFNFTIQIQRTLTVRNSDHEVLSDIMQNMPAIAWDNEAPTFALLIIPSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YI25</accession>
    <name>A0A9Q0YI25_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">BolA-like protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_37959</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the BolA/IbaG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YI25"/>
    <dbReference type="OrthoDB" id="4983at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990229">
        <property type="term" value="C:iron-sulfur cluster assembly complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.90:FF:000001">
        <property type="entry name" value="Transcriptional regulator BolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.90">
        <property type="entry name" value="BolA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002634">
        <property type="entry name" value="BolA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036065">
        <property type="entry name" value="BolA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050961">
        <property type="entry name" value="BolA/IbaG_stress_morph_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46229">
        <property type="entry name" value="BOLA TRANSCRIPTION REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46229:SF2">
        <property type="entry name" value="BOLA-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01722">
        <property type="entry name" value="BolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82657">
        <property type="entry name" value="BolA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8022928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="147" mass="15947" checksum="E6FD195B75643990" modified="2023-09-13" version="1">MNVGPLVQIFACSGRAIYLHRTCLIQCSLLSKMTKQPGPVEVTIAEKLGASLQPSHLDVTNESFMHNVPKGSETHFKVVVVSSNFENLSLIKRHRMVNDILKEELAGSVHALSIVAKTPSQWMTNSNIHKSPACLGGMAREKAGGPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YJI3</accession>
    <name>A0A9Q0YJI3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">LRAT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_35888</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YJI3"/>
    <dbReference type="OrthoDB" id="421951at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1720.10">
        <property type="entry name" value="endopeptidase domain like (from Nostoc punctiforme)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007053">
        <property type="entry name" value="LRAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46137">
        <property type="entry name" value="OS05G0310600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46137:SF3">
        <property type="entry name" value="OS05G0310600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04970">
        <property type="entry name" value="LRAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51934">
        <property type="entry name" value="LRAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="499"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="domain" description="LRAT" evidence="2">
        <location>
            <begin position="297"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8023439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="560" mass="63631" checksum="490CE085CB8F0166" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YJR6</accession>
    <name>A0A9Q0YJR6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Paraneoplastic antigen Ma1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_41840</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8020011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YJR6"/>
    <dbReference type="OrthoDB" id="115435at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00303">
        <property type="entry name" value="retropepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026523">
        <property type="entry name" value="PNMA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048270">
        <property type="entry name" value="PNMA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23095">
        <property type="entry name" value="PARANEOPLASTIC ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23095:SF17">
        <property type="entry name" value="PARANEOPLASTIC ANTIGEN MA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14893">
        <property type="entry name" value="PNMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="341"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="239"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="285"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8020011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="453" mass="50144" checksum="1D630F91BA3C5ACC" modified="2023-09-13" version="1">MSNPETESEKFDRELEDFLAQHGKKREDIQFPGSDTATKTVVEHIHKIEDRALKKHDYKRLRGFSGKSHVPSGELPFSTWYLHAEQMLSESSLSEEEKRLRLSECLSPPALTLYRRAAEELGPLATGAKLLEQLGKAFGVACEGEDLYTLFRDTYQEREEAASAYLLRLDERLDQAIQFGGVDRQQADKLRLSQFIRGCIFSEGLVSNLQLRQRKSRPPTLVGLLREVRIEEAEETARLTRRENARAPKKAQVNHAAIEKQQGKSDLNNLSKQLGDLTAQVAALQSAPSVKPADPPPTVKKDEASSASLLEAIKQLQKAVSDLQQGKNRRPEANRSKGVICYQCGERGHMLRDCTNAPNAELVQQRLLDGIECQALLDTGSQVTCVSSDFYRSRLSHRELMPLHDLGVTGAGGQDVPYLGYIQVDLTPSATDAGTGKTVTTLALVLPGSSALQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YK20</accession>
    <name>A0A9Q0YK20_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_38846</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YK20"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040287566" evidence="1">
        <location>
            <begin position="20"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8021597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="79" mass="9018" checksum="4C55732CCD997BD2" modified="2023-09-13" version="1">MRCFVFVCLVVLSTLLAQSYSTCSCGYCGSNGICHRDCMKACKGIKGGKRFWRQESQLETPVERYESVSIEEILRRLIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YL92</accession>
    <name>A0A9Q0YL92_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Tyrosine-protein kinase ABL2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_34471</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YL92"/>
    <dbReference type="OrthoDB" id="5581784at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004714">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00192">
        <property type="entry name" value="PTKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000554">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050122">
        <property type="entry name" value="RTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416:SF620">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR TORSO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040151288" evidence="10">
        <location>
            <begin position="22"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="353"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="424"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="458"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00051243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8024538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="711" mass="80422" checksum="FA5E2F244935BBF7" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YM93</accession>
    <name>A0A9Q0YM93_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Partner of bursicon</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_35218</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8025105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YM93"/>
    <dbReference type="OrthoDB" id="786951at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031395">
        <property type="term" value="C:bursicon neuropeptide hormone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001664">
        <property type="term" value="F:G protein-coupled receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005184">
        <property type="term" value="F:neuropeptide hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034441">
        <property type="entry name" value="Bursicon_suB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41151">
        <property type="entry name" value="PARTNER OF BURSICON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41151:SF1">
        <property type="entry name" value="PARTNER OF BURSICON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040473758" evidence="1">
        <location>
            <begin position="20"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8025105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="128" mass="14451" checksum="9577DE2AADCD8CD9" modified="2023-09-13" version="1">MSFLLILLLCAANGVLVRSRNERERCSLSEKHMHVNLQKFDSDRGQMVSCRGQITVSYCEGSCRSRTIPSVETFSGFKKMCRCCFESSTQTVRVPVPHCRIVGGRHLQHAIYFDIKQPTACSCARCQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YMF5</accession>
    <name>A0A9Q0YMF5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">DNA-binding protein D-ETS-4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_36792</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the ETS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8024146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YMF5"/>
    <dbReference type="OrthoDB" id="10057999at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000418">
        <property type="entry name" value="Ets_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046328">
        <property type="entry name" value="ETS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003118">
        <property type="entry name" value="Pointed_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849">
        <property type="entry name" value="ETS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849:SF182">
        <property type="entry name" value="SAM POINTED DOMAIN-CONTAINING ETS TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00178">
        <property type="entry name" value="Ets"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02198">
        <property type="entry name" value="SAM_PNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00454">
        <property type="entry name" value="ETSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00413">
        <property type="entry name" value="ETS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00251">
        <property type="entry name" value="SAM_PNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00345">
        <property type="entry name" value="ETS_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00346">
        <property type="entry name" value="ETS_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50061">
        <property type="entry name" value="ETS_DOMAIN_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51433">
        <property type="entry name" value="PNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PNT" evidence="6">
        <location>
            <begin position="99"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="ETS" evidence="5">
        <location>
            <begin position="282"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="193"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="194"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="268"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8024146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="368" mass="42303" checksum="4CC427501F19C968" modified="2023-09-13" version="1">MERHSLTQMSDSLYSFEDRHFQGFDMFPNNMMPPQDFSMGEKQQAMAQPMASPMSPPEGDMLTADATFLELENYYYSNQPSPTSTELSEQEMTEAYEIVMEDILSSITPRLGISTDPSQWSQEQAQAWISLLIQEYGRPKVNLSNFQVDGYTLSQMTQEELEQRAPGNGYLIHSNLNCLSKSIQREEIKPVLSPFQQQQQFQQQQQQQPPQPQAQPMSYDPNMMYPYPITSMPPPSPAATERELSPSPLASSSDEEEASMSSPARSPTGPNTSMTPHHTGSIQLWQFLQELLLQPDSYNYCIRFLDRSQGIFKIEDSVEVARLWGMRKNRPAMNYDKLSRSIRQYYRKGIMKKTEISQRLVYQFVTPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YNH8</accession>
    <name>A0A9Q0YNH8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_33371</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YNH8"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="163"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="197"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="264"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="334"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="398"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8025734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="425" mass="49357" checksum="3EAA21AFDC427A5F" modified="2023-09-13" version="1">MESETRPLVPREEDNRKQIPLLLMMVMWSLGIVEWSVPLDEEEGSARRSFIGNSKWRRRFVFMLSISYQIGIVFVSFAGALWYLVCYFGKTDHLIRFLSVVLCYWPTVMSLPMFCLAAFARNNRKRHDDGLTSIAWTNVFNESSFVRQFSYLNVSKMKPLPKIGVVIFFYGFASSISCFIVLLYVDMYKSCESWISYIWMVGEVIALFYFATFSYFLYLKRIILEEEGRRTLSFVKSNKGNLEICIVSVKSFFCNYMQLRNMLLPWLCIVMFSSTFGLTVFLTWAYKDKTSASNIIVQVNTTTQIPDSNMAAYIGRFCDYACDGYDILMRENTYIVYSVILVGKFFMPAAVTFFVVRGLDVKHVWDRLNVKLHLMFSTHNLEFWKRLTKYVEKLHPDVTFSAFAGLVIPVLGLATGLLGSHHFKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YPB6</accession>
    <name>A0A9Q0YPB6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HTH CENPB-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_36479</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YPB6"/>
    <dbReference type="OrthoDB" id="6754776at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="214"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="224"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="252"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8023907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="346" mass="38880" checksum="7C3FF1981C5D8FFB" modified="2023-09-13" version="1">MPRKYKRMTDRQSWGNDHLQQQWKQYFLVQQVSCRAAARNFGIPEATLCRRLKTPLITTNPPLGRAPALGAENEQRLKAHLVSMGSRGFGLTRLDVRRLAFQSAKRNNISDQHFNDQESGMAGDEWLNGFLKRNPDLSIRRAEGLSQARAMSFNRVVVTKYFDLLEKIAEEIGESAASIRNAVNGFRCCGLYPIDGGEVMDDEAAFIPAITSERTETEAAEQTLDAEIDQEQPQDAEIDQEQPQDAEIDQEPLQSQDALDTQAETEQPQDAGQLGQNNQRSVQVEVHQADTSSITFESLMPLPHYEHPRNRPKRKSLGTVVVTSSSYLQDLQTKQNAVKRPSVNVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YQN4</accession>
    <name>A0A9Q0YQN4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Organic cation transporter protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_31758</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YQN4"/>
    <dbReference type="OrthoDB" id="3936150at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.286.90">
        <property type="entry name" value="MFS transporter, transmembrane helix TM10b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24064">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="161"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="187"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="214"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8026815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="297" mass="34631" checksum="6B9F9253F83D1882" modified="2023-09-13" version="1">MKFDDVLVLIGEFGRYQRRMYFLCCLVGISIAFQQMLQVFHLGIDEEHWCAVEEFSNEVEQCYKDYRKTDVQQYKQCIDKYRNLSIPRKKDGSFSQCRKYDAVYDVGTEFVLVNYTYDISENKTISCDEGWVQDRSNLVRTINSDFELWCDREPKARISQMFFFAGVFFGSLFFGFMADWVGRCKTFFVTVTWLSLSSVLNAFAPTYWTYVLCRFLVATGNMGTFELAFVIGLVVQESARWLITKGRYSEAETIIRNAAKVNKTTDKLPPNFMEQLEKTAVVCSLWWAFGLGVFELQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YRB7</accession>
    <name>A0A9Q0YRB7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">5-hydroxytryptamine receptor 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_35378</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8025229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YRB7"/>
    <dbReference type="OrthoDB" id="10036964at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00637">
        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24228">
        <property type="entry name" value="B2 BRADYKININ RECEPTOR/ANGIOTENSIN II RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24228:SF72">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="29"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="66"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="109"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="150"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="204"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="270"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="296"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="45"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8025229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="388" mass="43994" checksum="53B9F4CAE024A1EB" modified="2023-09-13" version="1">MDFMNNTMDGMDISNERPYKIEEYPTQRIIIAILYGLLSLTGLLGNFLVILAVILSKKLRTITNVFVVNLSIADCLTCLMLPFNIVGLLNLNGDQEMYLLSENICKGAALILFTCAGCSVYTLAAIGINRWYLITRPVRRYRTFFAKGRITCMVFIVWCIPFLTAMLPPLLGLGELGYATKYASCTHKTSHPLSDYYSMLQSAIVYPIPLITIVSTYVLIFVHIREHAKTITEQPDISTTSYVSNAVSMDTNADLRKRLSRRQLEITRNLFYVVCAFFICITPYCIVLFIPPSDPLVPWTAAIFLVNSCVNPFIYATKHPHFKVIFKLILSCKCSQIPEKSRIMRTIRSMRSIRSIKGRRKQRNVEKRNAKIKAQIAGLSSRDSFGTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YT96</accession>
    <name>A0A9Q0YT96_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_32996</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YT96"/>
    <dbReference type="OrthoDB" id="10072364at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="208"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="227"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="244"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="279"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="312"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="337"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="353"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="373"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="419"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="434"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8025441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="451" mass="51515" checksum="6F1FF8ADC57ACD3D" modified="2023-09-13" version="1">MGIPKGSTPRSSGRLREKAQKKFEMPTRDHVLNAIQTTPGKSYEQYKPRKLINNFLKERNTYNNRDDPMAMTDDEEGLSDFIVEDEPPSFESRAKKRGKNRKKEKKRKKMQESSESSSEGMSDFIVEDEPPPSRQRRNKKTKKHTKTTEYSSESESDNKEVSSDFTEEDEESGIQQQSAKKTTKRMKRMLSSSDSESDKNDGVTPSRRSNRISRQPKKVFQKPNKKKAWENILEREPDLRETVDSDDMNDFVVGDEEVEEEVSDAETDVEANIDEDGIICEKSDTEKEGSKQRDDKFNNHENPEETDLPDSLADKLIKTDENLSKKQADIEKETEVRNQSTPSSFVSKKTDLAEAEGQSDDCGSDSECTTVGRKFRSKVRHRRQLSKALDSDASESDGEKINDTFVGRKRRRTSKNLNFDEDGDDSDDDSDYGGGRKEEMKGDEGEKGETR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YTK0</accession>
    <name>A0A9Q0YTK0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_31607</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YTK0"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1820">
        <property type="entry name" value="L1 transposable element, RRM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35555">
        <property type="entry name" value="ENDONUCLEASE-REVERSE TRANSCRIPTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35555:SF3">
        <property type="entry name" value="ENDONUCLEASE-REVERSE TRANSCRIPTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="6"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8026697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="200" mass="23353" checksum="A3292CFE0F072D58" modified="2023-09-13" version="1">MVDTAMKNISKELKKIKAEFGQAVEDLRKSVEELKGENKHLKESYLAIEKRAEKLEEEKRLHGAMINKRNNIRIVNLKSNEGESCLDLATVVLEEVGIPACKIERAHRDGRFVEGRERHLLVKVTFHQDMVQILKNARRALADKDYYIVEDLTKHDLKEKRKWRLQVQELYQNGTKLLFYNGAWRGLAGKPYDFQQQHAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1B8M8</accession>
    <name>A0A9Q1B8M8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Leucine-rich repeat-containing protein 69</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_44522</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000962">
        <property type="protein sequence ID" value="KAJ8017826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1B8M8"/>
    <dbReference type="OrthoDB" id="660555at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050715">
        <property type="entry name" value="LRR-SigEffector_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45752:SF195">
        <property type="entry name" value="LEUCINE-RICH REPEAT (LRR) FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45752">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="3">
        <location>
            <begin position="83"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8017826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="300" mass="33934" checksum="E0BFDE637346B186" modified="2023-09-13" version="1">MADTLLLRALKGQPTSVNLANKDIETVPKIISKLYRLQNLQLKNNKIRELPKEFSTLRTLTVLNLGNNKLEVLPEALTGLSNLEVLHLFGNNMQQLDGKVIGGLKNLKFLNINNNKLKSLPSAMNKLVHIQHLSVDGNQLTSLPSELCALSHLEELHVANNQLTSLPLEFGFLAKLKKVHLQKNKIKELPEGLSKCSNLQVIDVAGNDLRIFPSELHKMPLKELYCEENPLLDQLPVQSVQEDEVLTLKELTARYIVKQLKERWSFMRRAIRHYPDIRDMLAQASKCALCGGAFLNTWLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1B9C3</accession>
    <name>A0A9Q1B9C3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Fatty acid synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_42351</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000058">
        <property type="protein sequence ID" value="KAJ8019206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1B9C3"/>
    <dbReference type="OrthoDB" id="6926994at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004312">
        <property type="term" value="F:fatty acid synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05195">
        <property type="entry name" value="enoyl_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050091">
        <property type="entry name" value="PKS_NRPS_Biosynth_Enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43775">
        <property type="entry name" value="FATTY ACID SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43775:SF7">
        <property type="entry name" value="FATTY ACID SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="3" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="1" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="9">
        <location>
            <begin position="48"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8019206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="238" mass="25903" checksum="74718A025A92A450" modified="2023-09-13" version="1">MNIFKNGRWGSYRHQLLNEGNHYTSIPFFPEKFFTTPTNACVDVLTPGDFSSLQWIQSSSDPKSGEKYAVRYSSLNFRDVMLASGKLPRGSLPGKIINFAVEDLAHKECLIGMEFAGHNSEGKRVMGIVGSQGLAYHIFGEPCFLFDVPQSWTLEDAATVPLVYCSVFYALVVRGQVKAGESILIHSGSGGVGQAAIAVALSYGCKIYTTVGGYIVIFTLLNTIEKPAKTCQSTITPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1B9N3</accession>
    <name>A0A9Q1B9N3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_38778</name>
        <name evidence="1" type="ORF">HOLleu_43599</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000375">
        <property type="protein sequence ID" value="KAJ8018425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1B9N3"/>
    <dbReference type="OrthoDB" id="6154697at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8018425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8021538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="105" mass="12241" checksum="8D9654D45352D0F9" modified="2023-09-13" version="1">MTSILKDLDLPTLADRRKQNRLILFSKGFFGKANIPMDKLSHPTRKTRGMHNLHFCQLYSRSNCYKFSFLPSTLKDWNNLPATLIDGLDSHRDPIQYITNFIRAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BAI3</accession>
    <name>A0A9Q1BAI3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Angio-associated migratory cell protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_41209</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Plays a role in angiogenesis and cell migration. In smooth muscle cell migration, may act through the RhoA pathway.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8019565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BAI3"/>
    <dbReference type="OrthoDB" id="10261640at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000074">
        <property type="entry name" value="Angio-associated migratory cell protein-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024977">
        <property type="entry name" value="Apc4-like_WD40_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051179">
        <property type="entry name" value="WD_repeat_multifunction"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19857:SF8">
        <property type="entry name" value="ANGIO-ASSOCIATED MIGRATORY CELL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19857">
        <property type="entry name" value="MITOCHONDRIAL DIVISION PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12894">
        <property type="entry name" value="ANAPC4_WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 7" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="76"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="119"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="200"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="296"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="Anaphase-promoting complex subunit 4-like WD40" evidence="8">
        <location>
            <begin position="299"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="344"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="379"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00059273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00072425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8019565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="417" mass="45089" checksum="6C29CB8A5A92EF1E" modified="2023-09-13" version="1">MDPGDLDPGQEVDLNEDDVIEVIDEDGERNGGEIAVEMGDMDLEHYDEEEDDISNDEMLQEGALAPPASDDAETCFNAHKGSVFSCVLEPGGQSLAISGGEDDKAFVWNTSNGEVVLECSGHKDSVTCVGFNFDGTLAATGDMSGIVKVWKVETKKEIWSFEGSDLEWLRWHPLANVLLGGTSEGDVWMWKIPSGDCKMMQGHGCQSTCGKIMPDGKRCIVGYEDGSCKVWDLKDQVCLHHIKKGGHNHTDSITCIDCHKDNVMALTGSVDGTAKLVNTNTGKVVATFPAGDVDDTIGDTNSVECLEFSPNQSMVAVGSLNGLLSIWDIPTQKLRHHCRAETGIVRVRWDSASPVVCLGGLDGRVQLWDSRSGQRLSTYEGHSSEILDLDLSSDGNTILSASGDGTCRIFKVTQPER</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BBC9</accession>
    <name>A0A9Q1BBC9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Zinc finger CCHC domain-containing protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_42952</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000169">
        <property type="protein sequence ID" value="KAJ8018844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BBC9"/>
    <dbReference type="OrthoDB" id="10064617at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002218">
        <property type="term" value="P:activation of innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042509">
        <property type="entry name" value="ZCCHC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22639">
        <property type="entry name" value="GAG-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22639:SF3">
        <property type="entry name" value="ZINC FINGER CCHC DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="159"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="176"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="196"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="252"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="348"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="310"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8018844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="398" mass="44038" checksum="CE26E588F075CD1B" modified="2023-09-13" version="1">MASLQREASVQLQFEVGQSTEKVFDFLTRKGIDPGDHLLAVQGLPGHLWDITFKSVELRRKFWPVISDGECCSASVYTESTTLVTVLHVPHELDDNVVRYVLGRYGRVISGRFLMFKDYPTVFNGIRQYKMKLNSDIPSSVSFGGRSCWVRYRGQPRTCLKCGEAGHDAKECGVIRCFNCQSLGHASKECEVPTTCTICGKSGHHYRDCPVSFANKYKPNSSWIIGSHAKTSEGGEKEAEANLGEEVVEVSSDDATHSAIASNPGPSFMADSQPPNEVVSDKQTNDSQILLFEDSEPVVIPDTQASSDVVTDKKSSESEMDISDGRPEIKTLDNCIPQPQLQDNETCSNEIQSQPAMSVDKDPIFVKPRRKASSQHDTGRRPERSPVILLRGRGYSPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BCC5</accession>
    <name>A0A9Q1BCC5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Forkhead box protein B1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_42061</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000026">
        <property type="protein sequence ID" value="KAJ8019382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BCC5"/>
    <dbReference type="OrthoDB" id="5954824at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001766">
        <property type="entry name" value="Fork_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050211">
        <property type="entry name" value="FOX_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11829:SF142">
        <property type="entry name" value="FORK-HEAD DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11829">
        <property type="entry name" value="FORKHEAD BOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00250">
        <property type="entry name" value="Forkhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00339">
        <property type="entry name" value="FH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50039">
        <property type="entry name" value="FORK_HEAD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fork-head" evidence="3">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Fork-head" evidence="2">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8019382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="154" mass="17850" checksum="8E7AFD63D05C976D" modified="2023-09-13" version="1">MFTSTLWQKFPYFRNNERSWRNTILHNLSRNECFIKAGCSDDQRGHSWAIHPANADDLSRGDFRRRRARLRVGAVSDHEVLSYSYPCYPPSYPSTFVPSQIGYVPMTYSPLSTIFSSGITPYMTSRLPQHHPGMPTGYLPATRSQTPYLVYRHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BEA2</accession>
    <name>A0A9Q1BEA2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex</fullName>
            <ecNumber evidence="4">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_35900</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>(R)-lipoate</name>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the 2-oxoacid dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="4"/>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BEA2"/>
    <dbReference type="OrthoDB" id="537444at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045254">
        <property type="term" value="C:pyruvate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006086">
        <property type="term" value="P:pyruvate decarboxylation to acetyl-CoA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06849">
        <property type="entry name" value="lipoyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000010">
        <property type="entry name" value="Acetyltransferase component of pyruvate dehydrogenase complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.10:FF:000003">
        <property type="entry name" value="Acetyltransferase component of pyruvate dehydrogenase complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.320.10">
        <property type="entry name" value="E3-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003016">
        <property type="entry name" value="2-oxoA_DH_lipoyl-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001078">
        <property type="entry name" value="2-oxoacid_DH_actylTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045257">
        <property type="entry name" value="E2/Pdx1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036625">
        <property type="entry name" value="E3-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004167">
        <property type="entry name" value="PSBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23151">
        <property type="entry name" value="DIHYDROLIPOAMIDE ACETYL/SUCCINYL-TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23151:SF90">
        <property type="entry name" value="DIHYDROLIPOYLLYSINE-RESIDUE ACETYLTRANSFERASE COMPONENT OF PYRUVATE DEHYDROGENASE COMPLEX, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00198">
        <property type="entry name" value="2-oxoacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02817">
        <property type="entry name" value="E3_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47005">
        <property type="entry name" value="Peripheral subunit-binding domain of 2-oxo acid dehydrogenase complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00189">
        <property type="entry name" value="LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51826">
        <property type="entry name" value="PSBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2 4" id="KW-0450">Lipoyl</keyword>
    <keyword evidence="8" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="6">
        <location>
            <begin position="49"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="Peripheral subunit-binding (PSBD)" evidence="7">
        <location>
            <begin position="173"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="134"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="217"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="137"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="227"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8023450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="470" mass="50403" checksum="60C8F53502F1FCE2" modified="2023-09-13" version="1">MTSLASLSRAICTRNGRLSQVAWARLTPASQRWCFRSLSKSGCLLGVPGMRLEMPALSPTMTEGTIAKWLKQEGDAVSAGDALCEVETDKATVTMDSDEDGILAKILVPEGTTNVPIKTLIALMVEEGEDYKDVEIPSDTTPTADTPSDETAPVKEVEQAPAVSVEPGRARKNISPAVRQLLDTHQLDADSITATGPGGMLLKGDVLKYIEAGGKAAPAPAQLERPPVTPPLQPPTPVAQPAAPQPQETFTDIPLTGMRKVIAKRLTESKSTTPHAYASIDCCLDEVAKLRKQLKEDNMKVSVNDFIIKAVAMTLKQMPEVNASWKNDRVEQLKEVDISVAVATDNGLITPIVRDASHLGLTQISETVRELAGRARIGKLRPDEYQGGSFSISNLGMFGISEFTAVINPPQSCIMAVGGSQMTLNKQGKPSSFMTVQISSDARVIDDALASRFLSAFKKNMESPARLGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BEC3</accession>
    <name>A0A9Q1BEC3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Neuronal acetylcholine receptor subunit alpha-10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_35973</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the ligand-gated ion channel (TC 1.A.9) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BEC3"/>
    <dbReference type="OrthoDB" id="410315at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005230">
        <property type="term" value="F:extracellular ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18989">
        <property type="entry name" value="LGIC_ECD_cation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19051">
        <property type="entry name" value="LGIC_TM_cation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000028">
        <property type="entry name" value="AcetylCholine Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="235"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="264"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="295"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="6">
        <location>
            <begin position="21"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="7">
        <location>
            <begin position="240"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8023510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="441" mass="49809" checksum="FB4FA54C776C75BA" modified="2023-09-13" version="1">MKLTICFTAVYARHPRSAEGRVRDYLLNQANYSSKERPVLNSDDAVHVVLSMQLFAMLDLNERDEIVTTASWLNLAWRDERLTWTPQDFNNTDLVTMYAEEIWTPQIFLLNSLDSFGSSIASSNQGQILLTSEGNVLYGAPFIQASQCHMSIRYFPFDIQGCPLTFIPGNELAENIYITTELSRYQSSIKNSEWDLINITSHNYFYNVSHYISNRPVAEYTTGCLCMFFKRDPRYYITTLIIPSSLLCIMSMATFLAPPDSGERISLSVSMVLGLTVFQLLVADTLPTASKESPIVSTYLTINFVLACLTIPSSLANIGIAYGDCEEVSTCTKYSVYRNLFLEYLPRLLCVPTYRERIKCASVDVGTSNVFAEDIEMKAIVSISDGIEDKRKVHPVDSPTVKQKLSNAEKVGWTLFNSFICNNVLYSNRCSYIAIFIFIVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BFS5</accession>
    <name>A0A9Q1BFS5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_35015</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BFS5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8024950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="107" mass="11707" checksum="58D985B9892D8CA0" modified="2023-09-13" version="1">MYLPVGIYSKIVPFLIGSSITLVSKETFENLPEEIKQTRKKGKKTLWLADGSQIENKGTLHLDMEVGGAVLEHEVHITDIGSDAILGLELPNESSLFAGLAQRSNKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BGY5</accession>
    <name>A0A9Q1BGY5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_34755</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BGY5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="23"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8024754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="78" mass="8792" checksum="8E5FCF4776D58B6D" modified="2023-09-13" version="1">MESSRESNFNAAFLELSRSENCLRYVPIIAVLLSIIASLVVIRSWNQFLRYSTLQVYHCTIVNVHALPALGPDQANIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BH31</accession>
    <name>A0A9Q1BH31_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_33173</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BH31"/>
    <dbReference type="OrthoDB" id="6374566at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8025578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="100" mass="11620" checksum="CEAF520C6485B430" modified="2023-09-13" version="1">MTSLVRSLATPTVRLLNLRSRERGEREFTHTFLDLHIDSERSVGYAAKILARVILDRLIPTFAEEKLPKSQCSFRVNRGITDMIFVLRQIQEKCREQNMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BHU2</accession>
    <name>A0A9Q1BHU2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Sepiapterin reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_32925</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BHU2"/>
    <dbReference type="OrthoDB" id="153074at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004757">
        <property type="term" value="F:sepiapterin reductase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006729">
        <property type="term" value="P:tetrahydrobiopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051721">
        <property type="entry name" value="Biopterin_syn/organic_redct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44085">
        <property type="entry name" value="SEPIAPTERIN REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44085:SF2">
        <property type="entry name" value="SEPIAPTERIN REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0521">NADP</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8025382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="284" mass="31065" checksum="A7209AFC4A4CA5A2" modified="2023-09-13" version="1">MADTTQSFFGTPAVAIITGASKGIGRAIAIEFARRFSEGLLLVLTGRSEKDLQETASLIKADSSFLSSKMTVRCVVGDLQNQATIQNLIERLFENIDPSQYQHAILVNNAASTGDVSKYVRDFGLNDVKELQDYVFLNVNCPLILVSKFLSIFSSTIPDTSEKGPSKITIIQLSSNAALVPIKGWSMYCTGKAARAALHKIIAAEEPNVRVLSYSPGPVATDMLRYSRDNTRDPDTSRMTRNSYERSVQPIETVTVMCQVLEEDKYESGAHVSFTDIVGPPKNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BJB9</accession>
    <name>A0A9Q1BJB9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_29697</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8027683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BJB9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040251705" description="Secreted protein" evidence="2">
        <location>
            <begin position="23"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8027683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="96" mass="11494" checksum="CF222CC03BA990E9" modified="2023-09-13" version="1">MYHHQFFFFFFFFFFLSPLSRSRKKFSSNPSRASVFRQFYAKFLKPMRLDKSFLAIPRACQSRLATRNYGGKTARKSHLRRNSLLSPVDKRSPPNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BLC9</accession>
    <name>A0A9Q1BLC9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_31016</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BLC9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="60"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="163"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8028704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="192" mass="21646" checksum="F14E2087FC67704C" modified="2023-09-13" version="1">MTEVFNSTSPYTLRSNVRKYKQLIVLLRAFLAVLHFYAYHHNTIELSCTGTKMESCERMPILFTFLFGLGFLCTCYGFGSGTPPNACGHMIPLHYFNYTKSLPLSPQNGSAPFEVKVNKQKFGKGTEVEGVTPSAKRIRVFQPRNKSSSFRKVGTRICLSGGQAPEEGAATQTPYNPYRTRTVPHDRHKRKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BLN3</accession>
    <name>A0A9Q1BLN3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Glutaredoxin-2, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_28214</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Glutathione-dependent oxidoreductase that facilitates the maintenance of mitochondrial redox homeostasis upon induction of apoptosis by oxidative stress. Involved in response to hydrogen peroxide and regulation of apoptosis caused by oxidative stress. Acts as a very efficient catalyst of monothiol reactions because of its high affinity for protein glutathione-mixed disulfides. Can receive electrons not only from glutathione (GSH), but also from thioredoxin reductase supporting both monothiol and dithiol reactions. Efficiently catalyzes both glutathionylation and deglutathionylation of mitochondrial complex I, which in turn regulates the superoxide production by the complex. Overexpression decreases the susceptibility to apoptosis and prevents loss of cardiolipin and cytochrome c release.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Has a glutathione-disulfide oxidoreductase activity in the presence of NADPH and glutathione reductase. Reduces low molecular weight disulfides and proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Monomer; active form. Homodimer; inactive form. The homodimer is probably linked by 1 2Fe-2S cluster.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glutaredoxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8028952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BLN3"/>
    <dbReference type="OrthoDB" id="418495at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015038">
        <property type="term" value="F:glutathione disulfide oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03419">
        <property type="entry name" value="GRX_GRXh_1_2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000026">
        <property type="entry name" value="Glutaredoxin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011767">
        <property type="entry name" value="GLR_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002109">
        <property type="entry name" value="Glutaredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011899">
        <property type="entry name" value="Glutaredoxin_euk/vir"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014025">
        <property type="entry name" value="Glutaredoxin_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02180">
        <property type="entry name" value="GRX_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45694">
        <property type="entry name" value="GLUTAREDOXIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45694:SF18">
        <property type="entry name" value="GLUTAREDOXIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00160">
        <property type="entry name" value="GLUTAREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00195">
        <property type="entry name" value="GLUTAREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6" id="KW-0318">Glutathionylation</keyword>
    <keyword evidence="7" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Glutaredoxin" evidence="11">
        <location>
            <begin position="15"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00039819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8028952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="103" mass="11152" checksum="A3B0EDDD37582999" modified="2023-09-13" version="1">MVKAFVDAQIKDNKVVVFSKTYCPYCRMAKSALAAAGYEDYALLELDERDDGDAMQTYLAQLTGGRTVPRVFIGGKFVGGGSEVQALQQQGKLVPMLKEVGAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BLS4</accession>
    <name>A0A9Q1BLS4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Neuromedin-B receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_28204</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8028943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BLS4"/>
    <dbReference type="OrthoDB" id="10049706at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008188">
        <property type="term" value="F:neuropeptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45695">
        <property type="entry name" value="LEUCOKININ RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45695:SF26">
        <property type="entry name" value="NEUROPEPTIDE CCHAMIDE-1 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="105"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="145"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="189"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="242"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="282"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="10">
        <location>
            <begin position="44"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8028943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="407" mass="46209" checksum="9A56ABE44A58F058" modified="2023-09-13" version="1">MDSGNWSYAWIYAEFVNGTQPCPFSHSTVSNVFIIIILIIGIIGNVSLVLISLTHRDMRTPPNLMIVNLAMGDLVFLIMVVPSNVAFYNECMLILPKIMCKLHTAATFLTQGVSVYTLTALSVDRYQAIARPLRRRRGGNNHPKLRAFCTVVIIWTVSILFAAPAFKMAEVNPYGKECHLPFTTPETNIYFPIFFIGSYCIPLVAILVFYSLTAETLLHGLKHFNQGLRTGSDRRHKSRVKLAIIILIAALSFAICWLPHHIFTLWFEFCSFPGDIFSRPRIKILFILQQILPLLSCCLNPILLYAMSSNYRRKFRILLRETLHCNFGLLKAKEPLFRARTWTLISLRTTSLTRGSPSLNKPMTRQSSMDGHERDTPMNVNMFLFGRGTRTRMVLVSSKTAVILAIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BLY2</accession>
    <name>A0A9Q1BLY2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Titin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_28429</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BLY2"/>
    <dbReference type="OrthoDB" id="5969272at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26391">
        <property type="entry name" value="INACTIVE TYROSINE-PROTEIN KINASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26391:SF18">
        <property type="entry name" value="PROTEIN KINASE RECEPTOR TIE-1, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040269733" evidence="1">
        <location>
            <begin position="23"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="2">
        <location>
            <begin position="123"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="2">
        <location>
            <begin position="226"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="2">
        <location>
            <begin position="330"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="2">
        <location>
            <begin position="434"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="2">
        <location>
            <begin position="543"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="2">
        <location>
            <begin position="753"/>
            <end position="843"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8029107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="846" mass="94955" checksum="0E108BB67F15CFCC" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BPQ3</accession>
    <name>A0A9Q1BPQ3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Fragile X mental retardation syndrome-related protein 1-like B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_27270</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoplasmic ribonucleoprotein granule</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FMR1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BPQ3"/>
    <dbReference type="OrthoDB" id="424249at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045182">
        <property type="term" value="F:translation regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048170">
        <property type="term" value="P:positive regulation of long-term neuronal synaptic plasticity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045727">
        <property type="term" value="P:positive regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043488">
        <property type="term" value="P:regulation of mRNA stability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099577">
        <property type="term" value="P:regulation of translation at presynapse, modulating synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22425">
        <property type="entry name" value="KH_I_FMR1_FXR_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22426">
        <property type="entry name" value="KH_I_FMR1_FXR_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22427">
        <property type="entry name" value="KH_I_FMR1_FXR_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20402">
        <property type="entry name" value="Tudor_Agenet_FMRP-like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000053">
        <property type="entry name" value="Fragile X mental retardation syndrome-related protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008395">
        <property type="entry name" value="Agenet-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040148">
        <property type="entry name" value="FMR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022034">
        <property type="entry name" value="FMR1-like_C_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040472">
        <property type="entry name" value="FMRP_KH0"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041560">
        <property type="entry name" value="Tudor_FRM1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10603">
        <property type="entry name" value="FRAGILE X MENTAL RETARDATION SYNDROME-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10603:SF7">
        <property type="entry name" value="FRAGILE X MESSENGER RIBONUCLEOPROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05641">
        <property type="entry name" value="Agenet"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12235">
        <property type="entry name" value="FXMRP1_C_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17904">
        <property type="entry name" value="KH_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18336">
        <property type="entry name" value="Tudor_FRX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51641">
        <property type="entry name" value="AGENET_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Agenet-like" evidence="9">
        <location>
            <begin position="6"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="Agenet-like" evidence="9">
        <location>
            <begin position="65"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="380"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="194"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="395"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="414"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="429"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="464"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="519"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="549"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="583"/>
            <end position="597"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8030768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="657" mass="72582" checksum="FB45F81992C2D44E" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BSK9</accession>
    <name>A0A9Q1BSK9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_25462</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BSK9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8032051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="83" mass="9399" checksum="CAA57ECED2225EF0" modified="2023-09-13" version="1">MAARGLRRGPPIDVVHEKSVGQDEDMTLEASRGIQPGSKFYMSCFMFISLYGRIQGCLKRKHIDCMSSLRYNVLPVSRKAYTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BTQ7</accession>
    <name>A0A9Q1BTQ7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_26301</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="407598at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000032">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 297-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon gypsy-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054465">
        <property type="entry name" value="Integrase_p58-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF13">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22938">
        <property type="entry name" value="Integrase_p58_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="3">
        <location>
            <begin position="872"/>
            <end position="1030"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="341"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="605"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="705"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="78"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="341"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="356"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="380"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="605"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="649"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="673"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="721"/>
            <end position="745"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8032702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1372" mass="156711" checksum="6EE62512984E210F" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BUG0</accession>
    <name>A0A9Q1BUG0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_23083</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the PP2C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="3"/>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8032980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BUG0"/>
    <dbReference type="OrthoDB" id="10264738at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000222">
        <property type="entry name" value="PP2C_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832:SF803">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01032">
        <property type="entry name" value="PPM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0464">Manganese</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="11">
        <location>
            <begin position="23"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="181"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="537"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="199"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="210"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="224"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="240"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="269"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="283"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8032980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="557" mass="61426" checksum="338272B87D06F5C0" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BV65</accession>
    <name>A0A9Q1BV65_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_23542</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BV65"/>
    <dbReference type="OrthoDB" id="10056584at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38681:SF1">
        <property type="entry name" value="RETROVIRUS-RELATED POL POLYPROTEIN FROM TRANSPOSON 412-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38681">
        <property type="entry name" value="RETROVIRUS-RELATED POL POLYPROTEIN FROM TRANSPOSON 412-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="149"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="149"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="163"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8033337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="198" mass="22008" checksum="F06F7026F3B5D22F" modified="2023-09-13" version="1">MDSTAGFEHLPLVLLGIRSSIKEDLGHSASEMVFGTTLRLPGQFFDKPDGSALLDPSYYVHRLKSAMTSLIPTSPHVPTNRTTHVNANLNTCTQVFIRRDAVRKPLQPPYDGPFKVLERNDKVFQLDVNGRHETLSIDRLKAAHFEPDVDGPITDHSDPAPPVSIASSPSTTTTPVRTTRSGRRVYWRKRHLDPTATC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BWV3</accession>
    <name>A0A9Q1BWV3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_20948</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BWV3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011044">
        <property type="entry name" value="Quino_amine_DH_bsu"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50969">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="147"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8034207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="959" mass="108670" checksum="99A2D7DB0B6CFF1C" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BXA3</accession>
    <name>A0A9Q1BXA3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_21128</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BXA3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8034340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="69" mass="7976" checksum="5CC47EE76B577B77" modified="2023-09-13" version="1">MVFSDCQLFDLATVQTFINRLIPASRYTVCKIPVNSMLSVMFASIGVKYRNQEVKPWFLCDVCDPCFQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BXG9</accession>
    <name>A0A9Q1BXG9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Protein ALP1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_21203</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HARBI1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BXG9"/>
    <dbReference type="OrthoDB" id="10061326at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045249">
        <property type="entry name" value="HARBI1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027806">
        <property type="entry name" value="HARBI1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930:SF269">
        <property type="entry name" value="NUCLEASE HARBI1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13359">
        <property type="entry name" value="DDE_Tnp_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE Tnp4" evidence="8">
        <location>
            <begin position="29"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8034389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="190" mass="21055" checksum="D3C7B415FD80F9C5" modified="2023-09-13" version="1">MPVPDADLWKSVANQFETRSNFQNCIGALDGKHVVIQAPPNSGSMFFNYKGTFSIVLLALVDADYKFIAIDVGAYGRNSDGGIYASSSLGQMLETDTLDVPGPKVLPGAPHLGTVPHVIVADEAFPLKTYIMRPCPGRHLGEERKFFNYRLSRARRIVENAFGILRTKWQIYQRKIQQCNQSCEGNLCSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BXI5</accession>
    <name>A0A9Q1BXI5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Intraflagellar transport protein 57-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_21438</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IFT57 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BXI5"/>
    <dbReference type="OrthoDB" id="423881at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030992">
        <property type="term" value="C:intraciliary transport particle B"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005815">
        <property type="term" value="C:microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042073">
        <property type="term" value="P:intraciliary transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905515">
        <property type="term" value="P:non-motile cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019530">
        <property type="entry name" value="Intra-flagellar_transport_57"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16011">
        <property type="entry name" value="IFT57/HIPPI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16011:SF0">
        <property type="entry name" value="INTRAFLAGELLAR TRANSPORT PROTEIN 57 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10498">
        <property type="entry name" value="IFT57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3" id="KW-0969">Cilium</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="325"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8034552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="417" mass="48093" checksum="C0835890CB95E948" modified="2023-09-13" version="1">MGEEGRRGGSTEDGDVGPGQQFMHYVIMEDLIDKLKLLSYEERFLKSLGFKPLSRHYFAIPTNPGEQFFLFTSLCGWLINQCGNKFDMPEEYDDPNATIANIITEVRTIGASVDFPPAKLKPGYGEHCIYVVDKLADEALIKVGFKWDKPVYPEEELEEEAAIEDDVELTLEKVEDEIMEEDDIEEEESFMDLEGLGNLQNKRETQDSSRPEEIMLSNTDANQWKLEVERVTPSLKVHIRTDNKDWRTHVDQMHQHKEGIESALSETKVGYLDKLHKEISRTLEKIASREKYINNQLEHHLQEFRGVQDSLAEIKEQYRQASGGVTERTRALAEISEDLERVKQEMEERGSSMTDGSPLVKIKQALQRLKNEVTQMDVRIGVLEHSLMQAKLRDKNNLQKDLNRTVEEDEGYNFNTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-10-08" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C224</accession>
    <name>A0A9Q1C224_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">MICAL-like protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_17460</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8036818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
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        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="LIM_Mical_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="bMERB_dom"/>
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        <property type="entry name" value="Znf_LIM"/>
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        <property type="entry name" value="CYSTEINE-RICH PROTEIN CRIP FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46074:SF5">
        <property type="entry name" value="LIM DOMAIN-CONTAINING PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12130">
        <property type="entry name" value="bMERB_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DUF3585"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BMERB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0440">LIM domain</keyword>
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    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0862">Zinc</keyword>
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        <location>
            <begin position="488"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="domain" description="BMERB" evidence="7">
        <location>
            <begin position="1260"/>
            <end position="1347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="206"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="422"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="559"/>
            <end position="1255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1339"/>
            <end position="1402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="11"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="28"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="48"/>
            <end position="64"/>
        </location>
    </feature>
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        <location>
            <begin position="65"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="91"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="124"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="432"/>
            <end position="441"/>
        </location>
    </feature>
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            <begin position="464"/>
            <end position="475"/>
        </location>
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        <location>
            <begin position="589"/>
            <end position="602"/>
        </location>
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            <begin position="603"/>
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        </location>
    </feature>
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        <location>
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        <location>
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        <location>
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        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
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        <location>
            <begin position="925"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1001"/>
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        <location>
            <begin position="1033"/>
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        <location>
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        <location>
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        <location>
            <begin position="1377"/>
            <end position="1395"/>
        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
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        <source>
            <dbReference type="PROSITE" id="PS51848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8036818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1402" mass="156575" checksum="2B63BA97460C8CAC" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C4Y7</accession>
    <name>A0A9Q1C4Y7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Short-chain collagen C4</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="ORF">HOLleu_15980</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome 7"/>
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        <property type="term" value="C:collagen trimer"/>
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        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR24024:SF18">
        <property type="entry name" value="SHORT-CHAIN COLLAGEN C4-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0176">Collagen</keyword>
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            <begin position="1"/>
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        </location>
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            <begin position="43"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8038530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="251" mass="27383" checksum="706C0C6A32CAD5FF" modified="2023-09-13" version="1">MNRIVLVCLLSVVLCHVAWANLSDESPQNMRRENAKDVHEGEQQTQSKRTVSNCQNHNGASKGGSVYVRWGRRDCPEASDLLYSGIAAGSRHRDSPGGGANILCLPPDPSFVNPVDGVGGTRAFLYSIEYQLSNYPAWQQNHLDDVPCAVCKATGRFSHVMIPAKQTCPSSEWTLEYRGYLMAERSHPVHHKSMFVCVDHDPKVVPNSSSADNQNSGRLSLVEGKCSTTGGGLPCNTYPNGKEFTCAVCTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C5B5</accession>
    <name>A0A9Q1C5B5_HOLLE</name>
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        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_16148</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
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            <taxon>Holothuria</taxon>
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    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DoxX family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome 7"/>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="entry name" value="DoxX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040399">
        <property type="entry name" value="TMEM35A/B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13163">
        <property type="entry name" value="SPINAL CORD EXPRESSION PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TRANSMEMBRANE PROTEIN 35B"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="81"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="105"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8038662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="144" mass="15868" checksum="406A3CB0ED2954A6" modified="2023-09-13" version="1">MALVHYLTIILGSLMIYRGTFKLVPINLTETEGYDQVHKDFERFAKVFPLSVDPTLYCCLIGWVELVAGSVLVFGRQPYRALSALDILLVMLIALYTVAMTGETLALTCGVIFCAACALGIFFFRDALDEDARKNSGSVKSKST</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C722</accession>
    <name>A0A9Q1C722_HOLLE</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_16879</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C722"/>
    <dbReference type="OrthoDB" id="8042283at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8039229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="68" mass="7824" checksum="E5C47BE8F015E080" modified="2023-09-13" version="1">MKNLLSPENPTDRGYADLVKLIKHHQQSEPSIVVSRYKFHACTRETDILVIDYAAAHRKLADPCDFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C7J4</accession>
    <name>A0A9Q1C7J4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Retinol dehydrogenase 8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_13671</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8039620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C7J4"/>
    <dbReference type="OrthoDB" id="191139at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43391">
        <property type="entry name" value="RETINOL DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43391:SF86">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE_REDUCTASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8039620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="259" mass="28801" checksum="4DDFC4243A11C341" modified="2023-09-13" version="1">MASPSIKIVLITGTATGVGFTTALTFAGVPGKKYKVYATMRNMKTRKDELESAAGSMLNNTLFIRELEVGNEKSMVECVESILKEEKRIDLLINNAAIVHGGLFEQIDLGKDGTKHFQVNYFGPLRLMQLVIPSMKERKSGRILNISSIGTLLPCPFTDLYLHPKWALEGATECVAVTLRPFNVWASVVQLGAVMTQRAVQVQAAFDQYKDAFLKPPYIKNETDQEIFAKHAKNSMSLFTHETCQTPEEASNQCSHRNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C9K7</accession>
    <name>A0A9Q1C9K7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Sodium-coupled monocarboxylate transporter 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HOLleu_15013</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Nanhai2018</strain>
            <tissue evidence="15">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>iodide(out) + 2 Na(+)(out) = iodide(in) + 2 Na(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:71207"/>
            <dbReference type="ChEBI" id="CHEBI:16382"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C9K7"/>
    <dbReference type="OrthoDB" id="6132759at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015075">
        <property type="term" value="F:monoatomic ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098660">
        <property type="term" value="P:inorganic ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11492">
        <property type="entry name" value="SLC5sbd_NIS-SMVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="entry name" value="Sodium/glucose cotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018212">
        <property type="entry name" value="Na/solute_symporter_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051163">
        <property type="entry name" value="Sodium:Solute_Symporter_SSF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00813">
        <property type="entry name" value="sss"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42985:SF40">
        <property type="entry name" value="LD47995P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42985">
        <property type="entry name" value="SODIUM-COUPLED MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00456">
        <property type="entry name" value="NA_SOLUT_SYMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="11" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="49"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="79"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="123"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="154"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="184"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="235"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="275"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="320"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="376"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="404"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="435"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="519"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00036099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ8040655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="592" mass="63983" checksum="35E0659E935B061D" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C9N3</accession>
    <name>A0A9Q1C9N3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Ribonuclease P protein subunit p40</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_12685</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C9N3"/>
    <dbReference type="OrthoDB" id="63112at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030681">
        <property type="term" value="C:multimeric ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000172">
        <property type="term" value="C:ribonuclease MRP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000171">
        <property type="term" value="F:ribonuclease MRP activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013893">
        <property type="entry name" value="RNase_P_Rpp40"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15396">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15396:SF1">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08584">
        <property type="entry name" value="Ribonuc_P_40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8041778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="363" mass="40827" checksum="2540128E3D791EA2" modified="2023-09-13" version="1">MANFPAPKSNLIIERSSFKHQKSRHLAYISGHPLNYAVEVLLPGAKSLPDSLADVSPTLDDFFLIRDLPIVEFVGEDFITKIMKNGQFTALSVGTKVDNDMVVAVLPTGHLILSVDKDIYQVLGLSGKPSKFTGLDPARYMIQIDLFSENHKKGKKQYDRIIWALKDRLNLCFNFLLSWEPGDGNVAGLSQLTQLLDKYKMEDVSVKAADTKRKFSLIPELNSCQKLKEDESNGAHNIREVFEWIGTVSCDSNIAGGPEEYSSCYTCPEPCVEVKNLMCRQWSGLITVSRIQKMLTALRSFVMSDTSTPWAALTVHGFEDAPISWWKNEHGYHKDGSNLYTFVVFPDETYWLYLAVSTHDVCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C9V4</accession>
    <name>A0A9Q1C9V4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_12790</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C9V4"/>
    <dbReference type="OrthoDB" id="10057935at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027901">
        <property type="entry name" value="CFAP90"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34444:SF1">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34444">
        <property type="entry name" value="LOC361192"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15074">
        <property type="entry name" value="CFAP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8041868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="145" mass="16773" checksum="94BB22BB0D69039B" modified="2023-09-13" version="1">MEEQTDLHRKALEDYPGLMDDGQPVAAKSAFSYIPTRREQQPPERNVYNSTKLNQKAHSTYDQLFNQSDGYNNKLHRCDREHAKLRGLNVNDEENIKVVPTLSSTVYGHKLQQFNDPPGRKHVRIGHVNSEFYSRNGINIRGEEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CAK8</accession>
    <name>A0A9Q1CAK8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">leucine--tRNA ligase</fullName>
            <ecNumber evidence="3">6.1.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Leucyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HOLleu_11892</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Nanhai2018</strain>
            <tissue evidence="15">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>tRNA(Leu) + L-leucine + ATP = L-leucyl-tRNA(Leu) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:11688"/>
            <dbReference type="Rhea" id="RHEA-COMP:9613"/>
            <dbReference type="Rhea" id="RHEA-COMP:9622"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78494"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.4" evidence="3"/>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CAK8"/>
    <dbReference type="OrthoDB" id="15954at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004823">
        <property type="term" value="F:leucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006429">
        <property type="term" value="P:leucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07958">
        <property type="entry name" value="Anticodon_Ia_Leu_BEm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00812">
        <property type="entry name" value="LeuRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000060">
        <property type="entry name" value="Leucine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.730.10:FF:000011">
        <property type="entry name" value="Leucine--tRNA ligase chloroplastic/mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000100">
        <property type="entry name" value="probable leucine--tRNA ligase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002302">
        <property type="entry name" value="Leu-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00396">
        <property type="entry name" value="leuS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43740:SF2">
        <property type="entry name" value="LEUCINE--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43740">
        <property type="entry name" value="LEUCYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00985">
        <property type="entry name" value="TRNASYNTHLEU"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="ValRS/IleRS/LeuRS editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 12 15" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="13">
        <location>
            <begin position="59"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="13">
        <location>
            <begin position="475"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="domain" description="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding" evidence="14">
        <location>
            <begin position="753"/>
            <end position="884"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ8041154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="930" mass="107265" checksum="6A0A3B00C2ADE8AE" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CDQ0</accession>
    <name>A0A9Q1CDQ0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">phosphorylase kinase</fullName>
            <ecNumber evidence="2">2.7.11.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_09436</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 ATP + phosphorylase b = 2 ADP + phosphorylase a.</text>
            <dbReference type="EC" id="2.7.11.19"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Hexadecamer of 4 heterotetramers, each composed of alpha, beta, gamma, and delta subunits. Alpha (PHKA1 or PHKA2) and beta (PHKB) are regulatory subunits, gamma (PHKG1 or PHKG2) is the catalytic subunit, and delta is calmodulin.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.19" evidence="2"/>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8042634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CDQ0"/>
    <dbReference type="OrthoDB" id="419455at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005964">
        <property type="term" value="C:phosphorylase kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004689">
        <property type="term" value="F:phosphorylase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005977">
        <property type="term" value="P:glycogen metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002291">
        <property type="entry name" value="Phosph_kin_gamma"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01049">
        <property type="entry name" value="PHOSPHBKNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0321">Glycogen metabolism</keyword>
    <keyword evidence="6 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="172"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="1"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="345"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="349"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8042634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="364" mass="41234" checksum="DB4D43F703F5DEE3" modified="2023-09-13" version="1">MHLPKAQGISSTVRKCINRETQTECAVKIIDIFAAGSDSNEEVETVTEEYLNEVAILRKLCSEPGHPNIITLHDFMETPTYLFLVLELIIMKRVFEAVQYIHSHNIVHRDLKPENILLDECLNVKISDFGMAVQLRKGEILTELCGTPGYMAPEMLKTSMNIGGTVGYGKEIDLWACGVIMYTLLVGFAPFWHRKKMLMLRAIMEGQYQFNSPEWDDTSETAKDLIRKLLVVDPQERLTASQALDHEFFQQSEAVALSAFHPRRRFKAIIIIVYAVCSLQKYSLQKSVPVSRVCADPYGSKEFRKVLDTSAFAIYGHWVKKGERQNRAALFENVIKAELRRSASSSRSTTPNITPSSSFSNFIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CF69</accession>
    <name>A0A9Q1CF69_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">tRNA (uracil(54)-C(5))-methyltransferase</fullName>
            <ecNumber evidence="4">2.1.1.35</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_11078</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>uridine(54) in tRNA + S-adenosyl-L-methionine = 5-methyluridine(54) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42712"/>
            <dbReference type="Rhea" id="RHEA-COMP:10167"/>
            <dbReference type="Rhea" id="RHEA-COMP:10193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74447"/>
            <dbReference type="EC" id="2.1.1.35"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:42713"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.35" evidence="4"/>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CF69"/>
    <dbReference type="OrthoDB" id="10250660at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030697">
        <property type="term" value="F:tRNA (uracil(54)-C5)-methyltransferase activity, S-adenosyl methionine-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12439">
        <property type="entry name" value="RRM_TRMT2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.1070">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045850">
        <property type="entry name" value="TRM2_met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034262">
        <property type="entry name" value="TRMT2A_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010280">
        <property type="entry name" value="U5_MeTrfase_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45904">
        <property type="entry name" value="TRNA (URACIL-5-)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45904:SF2">
        <property type="entry name" value="TRNA (URACIL-5-)-METHYLTRANSFERASE HOMOLOG A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05958">
        <property type="entry name" value="tRNA_U5-meth_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51687">
        <property type="entry name" value="SAM_MT_RNA_M5U"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 6" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="155"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="616"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="12"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="43"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="555"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="427"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="477"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="527"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00033763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8043810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="645" mass="72279" checksum="E939DCD080630168" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CF95</accession>
    <name>A0A9Q1CF95_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Mediator of RNA polymerase II transcription subunit 31</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_10767</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the Mediator complex subunit 31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CF95"/>
    <dbReference type="OrthoDB" id="10257739at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1340:FF:000001">
        <property type="entry name" value="Mediator of RNA polymerase II transcription subunit 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1340">
        <property type="entry name" value="Mediator of RNA polymerase II, submodule Med31 (Soh1)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038089">
        <property type="entry name" value="Med31_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008831">
        <property type="entry name" value="Mediator_Med31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13186">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05669">
        <property type="entry name" value="Med31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0010">Activator</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 7" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="116"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="119"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="181"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8043595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="193" mass="23033" checksum="6B709B46A051A2E5" modified="2023-09-13" version="1">MNAETQDPEKLRFQVELEFVQCLANPHYLNFLAQRDYLKDSKFVSYLKYLQYWKEPQYAKFLKYPQCLHFLELLQYESFRKELANAQCVRFIEDQQLLHWQHYSYKRMRLQQSHIDNLQQQQQQQQPTGQQQNQQQSHMIAPQQQQPVSQAAGMPFGAVQHQAGQMAMQSQTRLLQPEQKPLQSLTQNSLNTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CG67</accession>
    <name>A0A9Q1CG67_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Tumor necrosis factor receptor superfamily member 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_07472</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CG67"/>
    <dbReference type="OrthoDB" id="100767at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01670">
        <property type="entry name" value="Death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000488">
        <property type="entry name" value="Death_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016729">
        <property type="entry name" value="FADD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15077">
        <property type="entry name" value="FAS-ASSOCIATING DEATH DOMAIN-CONTAINING PROTEIN FADD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00531">
        <property type="entry name" value="Death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00005">
        <property type="entry name" value="DEATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50017">
        <property type="entry name" value="DEATH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0675">Receptor</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Death" evidence="1">
        <location>
            <begin position="37"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8044677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="114" mass="13130" checksum="8520CE0DEB90D37A" modified="2023-09-13" version="1">MERQTQRMECQSIVSNNIDLEEEVNHTFLRDVSEEIAQWEGLASRLGLSNAKIHNIKEDYRGNNSEIKYQMLVSWKQKNGKNATYRALIEGLKKAGLEDLANEIQDGKYTDGLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CGV8</accession>
    <name>A0A9Q1CGV8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_08029</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CGV8"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8045097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="106" mass="12194" checksum="2EF33CF83DAE09FC" modified="2023-09-13" version="1">MNKGTEESNIHNIYGHSPNSAHINLEEQIFVHGSMMHFTIFMKDKPKDKNLSSSSDRHMKYDLCKNTSCTISDKGLGSHHLHTKSKVQEKFCWHLPMNNLQVSRVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CH95</accession>
    <name>A0A9Q1CH95_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Zinc finger MYM-type protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_07811</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CH95"/>
    <dbReference type="OrthoDB" id="7692318at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025398">
        <property type="entry name" value="DUF4371"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45749">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45749:SF21">
        <property type="entry name" value="DUF4371 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14291">
        <property type="entry name" value="DUF4371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4371" evidence="1">
        <location>
            <begin position="38"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8044920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="187" mass="21212" checksum="52B79D404EC4C141" modified="2023-09-13" version="1">MNATLAEELKKNCMIFIKILEHVKFLSRLGLAFQRNEECGNFLQLLNLRATDDPHVCDWKTKKRDKYTSHDIQNEIVRIMATDILRDIPDSISDAKFFSVLVDDECTDASNKEQLTICLRWTDQALEPNEDFVGFYEIPNITANTIVGAIKDVLIRLNLSLNNCRGQRFDVGGPMAGSRNGFAAQTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CK23</accession>
    <name>A0A9Q1CK23_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Adenosine receptor A2a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_04797</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CK23"/>
    <dbReference type="OrthoDB" id="9894375at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00637">
        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050569">
        <property type="entry name" value="TAAR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24249:SF372">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24249">
        <property type="entry name" value="HISTAMINE RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="44"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="119"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="165"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="206"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="261"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="10">
        <location>
            <begin position="60"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8046193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="350" mass="40464" checksum="91AABB297F6C819E" modified="2023-09-13" version="1">MASLQDYFSENVTAPSQLVTSLEYQIPRGYYHEVGTTFSPLITFVYVLVYFPIWCISIIDNGLVIFAFIENAYLRKIMNYPLVSLAVNDFLTGLVALPLHLTARLVQSRATCLESSRAILFLPSMVLFVSSVLHLIGIAVMRYVSLLRPMRFRSIVTEFRVKFTLATCWALSTLQFTFFLIGVSQAEENWICQGGVYKSVIAVHHFLLATLILPVVLIGMFCLYLFMVRKAFLQLKRRRRFCSQRSSQLIKRRQMEARSRFRGSVTMAIVVGAFSLCWMPTSFSYIFCTLKTHHLTRTVQSILSFLCEYPTFLNATLNPIIYAYRNSDFHDAFIHIWERFKVKWWSSRGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CK72</accession>
    <name>A0A9Q1CK72_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_04887</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CK72"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8046268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="68" mass="7990" checksum="AB45F66ADED09153" modified="2023-09-13" version="1">MGIDKDEYKFTRMQSCLDRYQPGLAVDVVIPCPKLVRYILATILQSCVQLLLQLRKHTPMELMIYQFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CMQ4</accession>
    <name>A0A9Q1CMQ4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Tumor suppressor candidate 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_06830</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CMQ4"/>
    <dbReference type="OrthoDB" id="9025707at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051881">
        <property type="term" value="P:regulation of mitochondrial membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029393">
        <property type="entry name" value="FUS1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15453">
        <property type="entry name" value="TUMOR SUPPRESSOR CANDIDATE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15453:SF8">
        <property type="entry name" value="TUMOR SUPPRESSOR CANDIDATE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15000">
        <property type="entry name" value="TUSC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8047751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="112" mass="12506" checksum="B5294DA384209766" modified="2023-09-13" version="1">MGGKASTYAWKAVSPVANYWYGDSSTSSATPGSMVAKGRYKVSPFVLKRTGSMYFDEDGHLAHEFYIEVPPSKNKKLKAGMKRIENNLTPQGEIEHDIPRLHVDLPMIMCQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CNH2</accession>
    <name>A0A9Q1CNH2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_00805</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CNH2"/>
    <dbReference type="OrthoDB" id="5876240at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8048476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="173" mass="19489" checksum="C789AF965E3B74E3" modified="2023-09-13" version="1">MPEAARRARTELDCFRLFFTRAVVDNLVKQTNLYAQQAGAGESSLGWTPVRRDDILAHDAIVTNLKRLRWRLGDGICPRTGCTRWESVPHVFWQCGFVPLLWEWFQVLAERLTFCNTWAVSQDYALYGLSPPSCNTSVRGILTFVSASIKLASLTRNPDMGLISGPDKQIGQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CP12</accession>
    <name>A0A9Q1CP12_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Kalirin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_00050</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8047929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CP12"/>
    <dbReference type="OrthoDB" id="10004999at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051336">
        <property type="entry name" value="RhoGEF_Guanine_NuclExch_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22826:SF211">
        <property type="entry name" value="LD43457P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22826">
        <property type="entry name" value="RHO GUANINE EXCHANGE FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="4">
        <location>
            <begin position="22"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="618"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="976"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1032"/>
            <end position="1106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1247"/>
            <end position="1272"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="372"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="1140"/>
            <end position="1207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="643"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="976"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="987"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1041"/>
            <end position="1054"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1056"/>
            <end position="1070"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8047929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1280" mass="144961" checksum="107FE87ADBDB5144" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CPN9</accession>
    <name>A0A9Q1CPN9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phospholipid scramblase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_00864</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">May mediate accelerated ATP-independent bidirectional transbilayer migration of phospholipids upon binding calcium ions that results in a loss of phospholipid asymmetry in the plasma membrane.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the phospholipid scramblase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CPN9"/>
    <dbReference type="OrthoDB" id="191150at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017128">
        <property type="term" value="F:phospholipid scramblase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005552">
        <property type="entry name" value="Scramblase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025659">
        <property type="entry name" value="Tubby-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23248:SF63">
        <property type="entry name" value="PHOSPHOLIPID SCRAMBLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23248">
        <property type="entry name" value="PHOSPHOLIPID SCRAMBLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03803">
        <property type="entry name" value="Scramblase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54518">
        <property type="entry name" value="Tubby C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0564">Palmitate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU363116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8048515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="253" mass="28935" checksum="B07C7F25F29783BE" modified="2023-09-13" version="1">MGDNSLTEPLLPLNQAPTLPAIPGCPPDLEYLTHLDQILIHQQVELIEVFTGWETENKYQIKNALGQQIYFAAEESELCMRLCCKSRRGFMMHIIDNLNQEVMRVVRPFQCCAGCCWCADSCDHCALTISVETPGGEQIGHVRQRGSRWKPHFDILDSSYEPVLKIRGPCFRTPCCIEDVNFQLMTYDESQQIGKISKQWAGITKEWFTKADNFGITFPQDLDVKVKGTLLGALFLIDFMFYEHDKNNKSFLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CQ29</accession>
    <name>A0A9Q1CQ29_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">P2X purinoceptor 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_00887</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CQ29"/>
    <dbReference type="OrthoDB" id="5798249at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046815">
        <property type="entry name" value="P2RX7_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36981:SF1">
        <property type="entry name" value="P2X PURINORECEPTOR 7 INTRACELLULAR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36981">
        <property type="entry name" value="ZGC:195170"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20478">
        <property type="entry name" value="P2RX7_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="P2X purinoreceptor 7 intracellular" evidence="2">
        <location>
            <begin position="32"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8048534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="190" mass="21462" checksum="DBEB84CD0602B88C" modified="2023-09-13" version="1">MAEPGPEPYAFEPVLRQPRPNVRPNARVARPNNSDEELEEEAERLPRRTDNLNWCVCQVHCTIMATERECLCCGEVEECLNLLDAHTSCITESQAFRSACLTEASLKAAIVLMRYAKGLTRPLDRYDNRVYRYAAYRSFTAWVHGHLGQGARKIIPSCVVTAIRQTWPSANGIYRGYLDAPEVDDALGNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CQP1</accession>
    <name>A0A9Q1CQP1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_02011</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CQP1"/>
    <dbReference type="OrthoDB" id="10370777at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="128"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8049315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="171" mass="18585" checksum="4851FDF73A0154A2" modified="2023-09-13" version="1">MYVNTKRSIFAFVGTSMAALALVLTLVGIQYDATAPLVTEQEVTEIILKRATPIWAGILLVLFGVRNLTLGGAVWRYKGIGCSDVCEAVLVNILALLAAGGCLGIIIWDALQEELFNDLTGNVDFVKFTTISFILTLEILWALVGCIAACIPVTREQKPEVEHGMENHAFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CQP6</accession>
    <name>A0A9Q1CQP6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_02450</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CQP6"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8049623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="124" mass="13418" checksum="7471D081D0EAFC90" modified="2023-09-13" version="1">MEEFNTFVDIDEEEDCDYLLEAKHILVQTSQYTAVGLPSSPSLPDTSIILDSSIEEDHAFVTSSASSSQSSLGEVGEGTNKKSESGGTTSKNIHDTDIDEEFELIFGDVNNKLISTGIVRGILQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CQT1</accession>
    <name>A0A9Q1CQT1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ovarian abundant message protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_02754</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CQT1"/>
    <dbReference type="OrthoDB" id="8939548at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040231522" evidence="1">
        <location>
            <begin position="19"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8049837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="101" mass="10997" checksum="7E00E3AEC820E7BD" modified="2023-09-13" version="1">MATFKLAILTMTIMMIKGIPVVEGIPVMEGIPVVEGIPVVEGIPVVEGIPVVEGIPVVKDIPVAVISLGHHMAVDYLALFDRKKRLGSRSLCCRNEKARHW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-10-08" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CQZ6</accession>
    <name>A0A9Q1CQZ6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Rho GTPase-activating protein 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_02911</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="9994905at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050770">
        <property type="term" value="P:regulation of axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008361">
        <property type="term" value="P:regulation of cell size"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007266">
        <property type="term" value="P:Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22207">
        <property type="entry name" value="pseudoGTPaseD_p190RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00159">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.440">
        <property type="entry name" value="FF domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002713">
        <property type="entry name" value="FF_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036517">
        <property type="entry name" value="FF_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057284">
        <property type="entry name" value="FF_RHG35_4th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039007">
        <property type="entry name" value="pG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051978">
        <property type="entry name" value="Rho-GAP_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032835">
        <property type="entry name" value="RhoGAP-FF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045786">
        <property type="entry name" value="RhoGAP_pG1_pG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039006">
        <property type="entry name" value="RhoGAP_pG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46005">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 190"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46005:SF4">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 190"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23083">
        <property type="entry name" value="FF_RHG35_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16512">
        <property type="entry name" value="RhoGAP-FF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19518">
        <property type="entry name" value="RhoGAP_pG1_pG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00441">
        <property type="entry name" value="FF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81698">
        <property type="entry name" value="FF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51852">
        <property type="entry name" value="PG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51853">
        <property type="entry name" value="PG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PG1 pseudoGTPase" evidence="5">
        <location>
            <begin position="586"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="domain" description="PG2 pseudoGTPase" evidence="6">
        <location>
            <begin position="775"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="4">
        <location>
            <begin position="1452"/>
            <end position="1639"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="939"/>
            <end position="1055"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1101"/>
            <end position="1139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1238"/>
            <end position="1318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1332"/>
            <end position="1387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1401"/>
            <end position="1445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="980"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="995"/>
            <end position="1007"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1009"/>
            <end position="1030"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1032"/>
            <end position="1055"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1267"/>
            <end position="1281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1282"/>
            <end position="1291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="1366"/>
            <end position="1378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="1423"/>
            <end position="1445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8049942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1671" mass="191164" checksum="ABDF86FDD407023C" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CR22</accession>
    <name>A0A9Q1CR22_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NACHT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_02264</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CR22"/>
    <dbReference type="OrthoDB" id="427518at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312">
        <property type="entry name" value="NACHT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312:SF2">
        <property type="entry name" value="NUCLEOTIDE-BINDING OLIGOMERIZATION DOMAIN-CONTAINING PROTEIN 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NACHT" evidence="1">
        <location>
            <begin position="69"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8049495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="644" mass="74961" checksum="36FBCD1CA955F421" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CRZ6</accession>
    <name>A0A9Q1CRZ6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Zinc finger MYM-type protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_02936</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CRZ6"/>
    <dbReference type="OrthoDB" id="10046160at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025398">
        <property type="entry name" value="DUF4371"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008906">
        <property type="entry name" value="HATC_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45749">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45749:SF21">
        <property type="entry name" value="DUF4371 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05699">
        <property type="entry name" value="Dimer_Tnp_hAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14291">
        <property type="entry name" value="DUF4371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4371" evidence="3">
        <location>
            <begin position="159"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="HAT C-terminal dimerisation" evidence="2">
        <location>
            <begin position="608"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8049960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="690" mass="79020" checksum="C3A964B0162CBF33" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CTC3</accession>
    <name>A0A9Q1CTC3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Histidine--tRNA ligase, cytoplasmic</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_04463</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8051041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CTC3"/>
    <dbReference type="OrthoDB" id="1906957at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004821">
        <property type="term" value="F:histidine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006427">
        <property type="term" value="P:histidyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041715">
        <property type="entry name" value="HisRS-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11476:SF7">
        <property type="entry name" value="HISTIDINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11476">
        <property type="entry name" value="HISTIDYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13393">
        <property type="entry name" value="tRNA-synt_His"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040422363" evidence="2">
        <location>
            <begin position="24"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="477"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="240"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="348"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8051041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="859" mass="97677" checksum="33D0BF61B8A80073" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CU98</accession>
    <name>A0A9Q1CU98_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Immunoglobulin subtype domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_04149</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CU98"/>
    <dbReference type="OrthoDB" id="6353782at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040397962" description="Immunoglobulin subtype domain-containing protein" evidence="3">
        <location>
            <begin position="21"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="238"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="165"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="180"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8050809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="305" mass="33567" checksum="7016E9E2830DE2BF" modified="2023-09-13" version="1">MDSTWLSALAFISLSSLIHTDGNSILRSSPKTVSFKLGHEGIIGCVCGDFTSLYWYSGNDTTAVIPLIYIDKSVKYGPGYSSGKYDISVNGSLVIKNVSSTHGGEYSVVIIEPGKSEQRVEIFTVYVEAKQQKDDELQQILRWSSLKVQDVPLLWSDKPITCDMSTGPEETGVMKTSNYDQERKPGNAERETTIAKDEPEKISASPVNENCEQFVEVADIKSLRAVIDQLKRIDRTTLALLVIVLFGIVLVLIATVVWRIYTPPLAKNPSCEEDKVACNHDLKRLLVSCPGSCPHGKSNESTDSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1GZA5</accession>
    <name>A0A9Q1GZA5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Tubulin alpha chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HOLleu_32723</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Nanhai2018</strain>
            <tissue evidence="16">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="13">Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the tubulin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1GZA5"/>
    <dbReference type="OrthoDB" id="10305876at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02186">
        <property type="entry name" value="alpha_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.600:FF:000005">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.20:FF:000001">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000007">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.600">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002452">
        <property type="entry name" value="Alpha_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01162">
        <property type="entry name" value="ALPHATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10 13" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 13" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="14">
        <location>
            <begin position="49"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="15">
        <location>
            <begin position="249"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ8027555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="451" mass="50483" checksum="AF82B9F3B4CD0446" modified="2023-09-13" version="1">MPEVISVHIGQAGAQVGNACWELYCLEHGIQPDGQRVEKEIISQERETFQSFFSESGSGKYVPRSVFIDLEPSVIDVIRTGPQRRLFHPEQLISGKEDAANNYARGHYSVGRDYVEQALDRLRKVAEQCTAFQGFLIFHSFGGGTGSGFSSLLLERLTQEYGSKKSKHTFVIFPSPHVSSSVVEPYNTVLATHASFDHSEITFMVDNEAIYEICRQNLDIERPSYANMNRLIAQVVSSITASLRFGGALNVDLSDFQTNLVPYPRIHFPLATYAPIMSIQKAHHEQFTVSDLTRACFEPSNLMVKCNLDHGKYMACCILYRGDVVPSEINAAIATLKTHRTIQFVEWRPVGFKVGINSRPPTAVPGGDLAKVNRSACMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFMEAREDLAVLEKDYEELGRDATNSDEGTDDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1GZN0</accession>
    <name>A0A9Q1GZN0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transmembrane protein 144</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_29790</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM144 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8027750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1GZN0"/>
    <dbReference type="OrthoDB" id="426527at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015144">
        <property type="term" value="F:carbohydrate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3730.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010651">
        <property type="entry name" value="Sugar_transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012435">
        <property type="entry name" value="TMEM144"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16119">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 144"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16119:SF17">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 144"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07857">
        <property type="entry name" value="TMEM144"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="53"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="78"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="107"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="140"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="221"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="259"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="291"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="318"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="350"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8027750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="372" mass="40422" checksum="CE8C3D9CEEEF1C27" modified="2023-09-13" version="1">MPILNQLFGEDDLLDQEGPSTFTVGLIATVVASVGFGCNLVPVKKVHTGDGMFYQWIECAAIWCVGLVVQILRDNPPFYPLAMLGGIFWTTGNICVVPCLKTIGMGLGILLWGSTNLLVGWACGHFGLFGLKKDEITRPLLNYFGLGFALLRLILFLFVKSDAGISTNSSVAPQRSVLVHDDDDPLLIPPHLQTGSVENISQSLKDDKSWVDKLSPIQKRLIGCTLALISGVLYGLNFVPCLYIQQNFEGAPGSGLDYAFSHFSGIFSSSTAYFLVYSSYMQNRPQLPPNLVWPSAACGIIWAIAQLGWFVATYDLSLSVSFPIVTSGPAVIAAAWGVFYFREIRGWKNYIVLCFGVLTVVAASVLSALSKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H1H0</accession>
    <name>A0A9Q1H1H0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">DNA polymerase iota</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_28475</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H1H0"/>
    <dbReference type="OrthoDB" id="447129at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019985">
        <property type="term" value="P:translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1170.60:FF:000006">
        <property type="entry name" value="DNA polymerase iota"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000013">
        <property type="entry name" value="Polymerase (DNA directed) iota"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053848">
        <property type="entry name" value="IMS_HHH_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001126">
        <property type="entry name" value="UmuC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46404">
        <property type="entry name" value="DNA POLYMERASE IOTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46404:SF1">
        <property type="entry name" value="DNA POLYMERASE IOTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00817">
        <property type="entry name" value="IMS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21999">
        <property type="entry name" value="IMS_HHH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50173">
        <property type="entry name" value="UMUC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0237">DNA synthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UmuC" evidence="3">
        <location>
            <begin position="72"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="13"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="31"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8029145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="350" mass="38747" checksum="D7E0AD70D2207BE1" modified="2023-09-13" version="1">MALQMKRSWNELASDDEADDPSDEQETEDWSASSAVVLSPPSSKDTSQPYYGASSKCVPQAHSNQNEHKRTIVHVDLDCFYAQVEMIRNPELRTKPLGIQQKNIVVTCNYVAREYGVTKLMYIKDAKEKCPNLVLVSGEDLTHYREYSQRVSDLLQSEFSTLVEKLGFDENFVDVSSLVEDRLKAYQGKPKMEVKGHIFGSENEGSSSQCSCGCHQRLAVGSQIAGEMRERLKEVIGLTGCAGIAWNKLLAKLVGERHKPNDQTTLFPGREQQLMIGLEKARRIPGIGHSMNKKLSTLGITTVLELQKASSAELEAVFGSQITAMMKNSSFGIDKSPVNKTGPPQVLKAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H1X3</accession>
    <name>A0A9Q1H1X3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_26368</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H1X3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="110"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8030088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="135" mass="15078" checksum="3E0F807F8B131B2A" modified="2023-09-13" version="1">MTMGCTLLKFEIHCGDGGNQKFTGETINTCHQILMEAVNKAREGPQKVRSWCAEDTRIDYNALQRSREGFQRRLEFEGPGTRLTFNTMADSDKLRVFLNQTNASQLQPLASSMSSLDDLSTNDGDADNSDHIDVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H2G4</accession>
    <name>A0A9Q1H2G4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_22799</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM200 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8032757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H2G4"/>
    <dbReference type="OrthoDB" id="9994280at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018787">
        <property type="entry name" value="DUF2371_TMEM200"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31815">
        <property type="entry name" value="AGAP005329-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31815:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 200C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10177">
        <property type="entry name" value="DUF2371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="100"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="155"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="50"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="329"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="378"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="526"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="52"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="334"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="378"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8032757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="569" mass="63651" checksum="CA282DDD1BA0BBE0" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H376</accession>
    <name>A0A9Q1H376_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Lysosome-associated membrane glycoprotein 5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Lysosome-associated membrane protein 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">HOLleu_27408</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="24">Nanhai2018</strain>
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        <subcellularLocation>
            <location evidence="4">Cell projection</location>
            <location evidence="4">Dendrite</location>
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    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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            <begin position="141"/>
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</entry>
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            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
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        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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</entry>
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        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="775"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="801"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="813"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="826"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="889"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="919"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="930"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="971"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="990"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1023"/>
            <end position="1039"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1046"/>
            <end position="1061"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1159"/>
            <end position="1185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1214"/>
            <end position="1224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1225"/>
            <end position="1241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1293"/>
            <end position="1310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1319"/>
            <end position="1341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1357"/>
            <end position="1372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1373"/>
            <end position="1386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1387"/>
            <end position="1398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1400"/>
            <end position="1428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1454"/>
            <end position="1478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1488"/>
            <end position="1524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1572"/>
            <end position="1584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1585"/>
            <end position="1604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1636"/>
            <end position="1658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1678"/>
            <end position="1720"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1752"/>
            <end position="1763"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1777"/>
            <end position="1790"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1791"/>
            <end position="1801"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8034827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="2107" mass="227219" checksum="B903FC7BF245AAFC" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H4I5</accession>
    <name>A0A9Q1H4I5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_23168</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H4I5"/>
    <dbReference type="OrthoDB" id="9909359at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8033044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="64" mass="7407" checksum="39E09039210677B4" modified="2023-09-13" version="1">MKNNKSPGPDGFTVEFYKYFWNEIGQFLLRSLNFGYENSLSVTQKQGVITLIPKGDKPRCYLNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H543</accession>
    <name>A0A9Q1H543_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_26300</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H543"/>
    <dbReference type="OrthoDB" id="10072345at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00229">
        <property type="entry name" value="SGNH_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12690">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013830">
        <property type="entry name" value="SGNH_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13472">
        <property type="entry name" value="Lipase_GDSL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="2">
        <location>
            <begin position="286"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8032701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="323" mass="37550" checksum="8A6B7C4E69E7E922" modified="2023-09-13" version="1">MIIGDSLLKYVNQEDFSNDNVTVKCLPGATVKDVKDVLQTHLDEMESISHLVIHVGTNDIAASPTPVIDDITVEFEDLVTNIQLQGDHTPVIFLSGPCPRSDKHMPQISELNSRLMMLADKLDCKFLNNLASFTYGDGEIDMSFFNSDGLHLNRKGTKKLLNKLTTIGPICLRNGESSNLNTHNHRSHRNDNFANKYRYRQPRPTRLPIQPDEQYDRKYDLVSRYEPVESYRDHRLDRGRHPIEMDQNHNSRTYQSTPTRRYADRYNGNRSLYQPHYQRYPSNRGCYNCGEFNHVVTNCRYDRPLKCRLCSRLGHKERNCLFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H5N1</accession>
    <name>A0A9Q1H5N1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Fibrinogen C domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_24095</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H5N1"/>
    <dbReference type="OrthoDB" id="6145874at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00087">
        <property type="entry name" value="FReD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19941">
        <property type="entry name" value="TIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050373">
        <property type="entry name" value="Fibrinogen_C-term_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025615">
        <property type="entry name" value="TILa_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040941">
        <property type="entry name" value="GGGWT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143">
        <property type="entry name" value="FIBRINOGEN/TENASCIN/ANGIOPOEITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12714">
        <property type="entry name" value="TILa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00186">
        <property type="entry name" value="FBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="81"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="3">
        <location>
            <begin position="117"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8033748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="357" mass="40102" checksum="B2BD59C779090C9F" modified="2023-09-13" version="1">MVFGHCECQPTCNIPQNTTRCISSCDATESCICADGFLIKGNDCVSPNECGCYAPELYTEILNGDSFVNFKCSEKCTCNDDQLHCNSNFECSPNATCKIENGVRNCYCNEGYQGNGEICSPLPTDCYDAYEAGHGDNGVYTILPSGWPGSPFKVSCVMSTNGGGWTVFQRRTDGVTDFYQNWTSYRYGFGSLEGEFWLGNEHLHYLTNQKNYTLRIDIVTSEGSSVYDEYLYFRISNESNKFRIDNIGTHNGTAGNGMYNSGGYLFSTYDQDNDGCGNHQCAKVHRGAWWYANDWCPKCLNRHCHNFRYNSTCSGQCTASNLNGEYNGGNGENIFWANDYSYCNLIFTEMKIRPFEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-04-02" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H6P2</accession>
    <name>A0A9Q1H6P2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_21257</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="8048386at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008737">
        <property type="entry name" value="DUF1758"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005312">
        <property type="entry name" value="DUF1759"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040676">
        <property type="entry name" value="DUF5641"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008042">
        <property type="entry name" value="Retrotrans_Pao"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331:SF1">
        <property type="entry name" value="GAG-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05585">
        <property type="entry name" value="DUF1758"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03564">
        <property type="entry name" value="DUF1759"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18701">
        <property type="entry name" value="DUF5641"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05380">
        <property type="entry name" value="Peptidase_A17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="8">
        <location>
            <begin position="1462"/>
            <end position="1636"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="315"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="421"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="317"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8034425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1808" mass="203236" checksum="F5FDDE1FFA7933AA" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H791</accession>
    <name>A0A9Q1H791_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Sushi, von Willebrand factor type A, EGF and pentraxin domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_15615</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H791"/>
    <dbReference type="OrthoDB" id="6127264at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sushi" evidence="2">
        <location>
            <begin position="34"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="2">
        <location>
            <begin position="98"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8038247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="178" mass="20525" checksum="73AEC47294B471AE" modified="2023-09-13" version="1">MKNKNNNSNFYSFLQQTIGRLKFYFIFTVISVPCDHETLTNGNVTYTLDGITHRRPVHGVIRIFNCDTGFTIDEDEPSLCDAGNWTRGLPNCRDSRCCIPPHPSYGRFKLPNNYYYTSNTFVTSGTTLAYRCSSGYFKCLQIKPVCKDGEWEPNIRNLCVPSKYSLLLPYRRCNLSRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H792</accession>
    <name>A0A9Q1H792_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_22065</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H792"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="85"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8034996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="105" mass="12222" checksum="99499925EED20E84" modified="2023-09-13" version="1">MDNQTDNVFWENTPKTPLEFLKTLLRRTLHRYQEFSWKTLVEGCNVIMIKQDIIIRINRTFGTLPSYQTFRIVWNRKSTSKTLTTKTLTSTTSTSASQPTTNFAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H8Q4</accession>
    <name>A0A9Q1H8Q4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_17953</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H8Q4"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8037199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="88" mass="10175" checksum="3C5D4E4503C16930" modified="2023-09-13" version="1">MPHIEWKNPIVFGGGQRSPGVTGGQTLKTVLTQYLEAGSLDEFHTLYVDALWYEEEPYHFLWRRKVIWGHRGSNLENFVNTISQEGKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H9S8</accession>
    <name>A0A9Q1H9S8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Orexin receptor type 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_15658</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H9S8"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00637">
        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45695:SF9">
        <property type="entry name" value="LEUCOKININ RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45695">
        <property type="entry name" value="LEUCOKININ RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="167"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="9">
        <location>
            <begin position="66"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8038279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="265" mass="30660" checksum="D153CF2DF3C36F68" modified="2023-09-13" version="1">MIVQRLIDFELDRVSRDNFSDESSISSFPYISDYFIEKDFCEYNRSKGSEWAAILSFIFVVLGVSTSTAYLIVTFRHSSAALTSNFPFLNNFLLASFINLISNGIFDIHVIKNGCWHGGRLLCKVLPTISDTSENAMVMFMVAIILEQNHPYVRSFLSKKTKFRKRLTITVLWLVTVVFSIPEVIIWDIFEFYESQFCTIRVTRYHHYGMYVQKGLFLKYVVPLLLLSCCDVSTCSCSFAKGIQHANIKPSTCRELFEKWRQRTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HA76</accession>
    <name>A0A9Q1HA76_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_19175</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8038173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HA76"/>
    <dbReference type="OrthoDB" id="10070636at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8038173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="240" mass="26847" checksum="AC1D3E9E845E59EF" modified="2023-09-13" version="1">MTVTEHLGGIKISDILAKDISVTNYRISKNEETLSKAEFSEGEKTHGGSDLILKGCQAPVKVKEPGESTDKRTESDKDIIDPERSKVTPDDETSNYDVSGVWHDDVDNSEYSGPSEGLSNEKESSVCSSLNEEERNIEKEHPQSDYVITEKILQKLQPPVPHVIWPENKFMTQHTAFVYGSPSRLAPVIVPVHSVDCPVSKMFLPSNRGTVCPPNSPTSFLNKEYGFYPVERHFPPSRDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HAT9</accession>
    <name>A0A9Q1HAT9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_16003</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HAT9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8038551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="58" mass="6452" checksum="8C449353178A96F7" modified="2023-09-13" version="1">MNYEMHLTETASVDSNEGAMSVSKCSTSPKDNLNLPRHTDTGRPDISSPHLHVKRKET</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HCQ8</accession>
    <name>A0A9Q1HCQ8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_11988</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HCQ8"/>
    <dbReference type="OrthoDB" id="10540132at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040326772" evidence="1">
        <location>
            <begin position="22"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8041235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="70" mass="7405" checksum="B5C2CE4206281B55" modified="2023-09-13" version="1">MAARTGMKFTSSLLLIAEVLSKSFRQSNAEEVSRKDTGALVNILGPVACLAYDQFPKVAVRASGTEANSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HDI7</accession>
    <name>A0A9Q1HDI7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Tenascin-R</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_13011</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8042045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HDI7"/>
    <dbReference type="OrthoDB" id="6145874at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050373">
        <property type="entry name" value="Fibrinogen_C-term_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040941">
        <property type="entry name" value="GGGWT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143">
        <property type="entry name" value="FIBRINOGEN/TENASCIN/ANGIOPOEITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00186">
        <property type="entry name" value="FBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="1">
        <location>
            <begin position="72"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="1">
        <location>
            <begin position="123"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8042045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="219" mass="24541" checksum="C80BECF4B31AE1A8" modified="2023-09-13" version="1">MKKNSVRSPEVPSTYLCDEGEILVSSRCDKLFSCKGNKIYLNESYQCDAQATCDKREAGRRCYCNKRYAGDGQTCTRIVDCYQLLRDGETTSGVYTVFPVNWPNKTLNVSCDMTTDGGGWTNFHVASEAGLYALGVSGYNTSSTAGNSLSLHDQMNFSTFDMDNDVSRNLNCAKMYSGGWWYNECSFANLNSNTQSQWMLNYYNDGINKTELKLRPKGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HFI9</accession>
    <name>A0A9Q1HFI9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SCAN box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_07000</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HFI9"/>
    <dbReference type="OrthoDB" id="69656at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF13">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCAN box" evidence="2">
        <location>
            <begin position="194"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8047877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="274" mass="32965" checksum="634CE457068DC429" modified="2023-09-13" version="1">MRKDEIKRYVLEYLVDESILPITVLETAITVPTDNTFELKRLEIEMNKEIRLKEMERKKEKEEREMQMQKEKEEREMQMQREKEAREHEFRLKQLELGVIKASDPKIGLDTGGFDVSKHVKFVPKFQEDNVEKFFNHFEKLGEQLKWPRDKWSILIQSNFTGKAQEVYSALSIEDSMDYDKVKKAILQAYELVPEAYRQKFRKYRKADTQTYVEFAYQKERHFDRWCASKKVSTFDTLRQLMLVEEFKRCVNDDIKTHLEENKADNLKEVANLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HKS3</accession>
    <name>A0A9Q1HKS3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_04362</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the apolipoprotein L family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HKS3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042157">
        <property type="term" value="P:lipoprotein metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008405">
        <property type="entry name" value="ApoL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013162">
        <property type="entry name" value="CD80_C2-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14096">
        <property type="entry name" value="APOLIPOPROTEIN L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14096:SF28">
        <property type="entry name" value="APOLIPOPROTEIN L, 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08205">
        <property type="entry name" value="C2-set_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="369"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="498"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="527"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="145"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="618"/>
            <end position="652"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8050969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="805" mass="89668" checksum="470ECA1090E84B3A" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HLH4</accession>
    <name>A0A9Q1HLH4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_02686</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HLH4"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040234819" evidence="1">
        <location>
            <begin position="22"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8049786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="67" mass="7723" checksum="C75A68AB3985AE68" modified="2023-09-13" version="1">MHSGPAIFCLLLLIFSATSHGRPWQDADDDVIYEAIVKRVVDDLTSYQESPAKKRDICWLFFTPYQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HLZ5</accession>
    <name>A0A9Q1HLZ5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Profilin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_04645</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the profilin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8051180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HLZ5"/>
    <dbReference type="OrthoDB" id="421374at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.30">
        <property type="entry name" value="Dynein light chain 2a, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048278">
        <property type="entry name" value="PFN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005455">
        <property type="entry name" value="PFN_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036140">
        <property type="entry name" value="PFN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45134">
        <property type="entry name" value="OS08G0543275 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45134:SF5">
        <property type="entry name" value="OS08G0543275 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00235">
        <property type="entry name" value="Profilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00392">
        <property type="entry name" value="PROF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55770">
        <property type="entry name" value="Profilin (actin-binding protein)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040337296" description="Profilin" evidence="4">
        <location>
            <begin position="24"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="58"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="63"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8051180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="401" mass="45965" checksum="27A5522AA334700F" modified="2023-09-13" version="1">MANFRLKLIFDLILFKWVEKVRAIPYLPTYLPTYLPTYLPTYLPTYLPTYLPTYLPTYLPTDRPTDRPTDRPTDRPTDRPTDRPTDRPTDRPTDRPTDRPTDRPTDRPTDRPTHPPTHPPTYLPTYLPTYLPTYLPTYLPTYLPTYLPTYLPTYLPTYLPTYLPTYLPTYPPNLTIYLPTHLPTYPPIHLSTYPLTHLPTYPPIHLPTYPPTYLPIYQPTYPPNLPTYLPTYPPNLPTYLPTYLPTYLPTSLSSFLPPFLPPSPLPTYLLPIHPPHHLPTYPLTRLPVYQLPTYPPTHPPTIVINVDALYPAEAEHIAACLKSKNFRAFTANGMFCEGVKYSFLKEEDEKLMFANSKSEGAITLQASKTAIVIAHGPEGCQQENLNKAVGVIAKYLESLDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YA82</accession>
    <name>A0A9Q0YA82_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Cysteine dioxygenase</fullName>
            <ecNumber evidence="3 9">1.13.11.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_43914</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-cysteine + O2 = 3-sulfino-L-alanine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20441"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:61085"/>
            <dbReference type="EC" id="1.13.11.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="9">Binds 1 Fe cation per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Organosulfur biosynthesis; taurine biosynthesis; hypotaurine from L-cysteine: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the cysteine dioxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.20" evidence="3 9"/>
    <dbReference type="EMBL" id="JAIZAY010000527">
        <property type="protein sequence ID" value="KAJ8018220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YA82"/>
    <dbReference type="OrthoDB" id="543511at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017172">
        <property type="term" value="F:cysteine dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019448">
        <property type="term" value="P:L-cysteine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042412">
        <property type="term" value="P:taurine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010300">
        <property type="entry name" value="CDO_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12918">
        <property type="entry name" value="CYSTEINE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12918:SF1">
        <property type="entry name" value="CYSTEINE DIOXYGENASE TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05995">
        <property type="entry name" value="CDO_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9 10" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="8 9" id="KW-0408">Iron</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0883">Thioether bond</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU366010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8018220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="70" mass="8120" checksum="522086851186A449" modified="2023-09-13" version="1">MERRFIKSPSSLDELIESLHVLFESDKVNVEEVQEVMENYKSNEDHWGKFADYDHNVHLVSSNGNGLGRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YCB4</accession>
    <name>A0A9Q0YCB4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Reverse transcriptase RNase H-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_44554</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000984">
        <property type="protein sequence ID" value="KAJ8017804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YCB4"/>
    <dbReference type="OrthoDB" id="4369127at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041373">
        <property type="entry name" value="RT_RNaseH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17917">
        <property type="entry name" value="RT_RNaseH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Reverse transcriptase RNase H-like" evidence="7">
        <location>
            <begin position="7"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF17917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8017804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="64" mass="7349" checksum="03B0441996048434" modified="2023-09-13" version="1">MGNYREVEVLKTYLTGAKISVVYTDHNPLVHLSSAQLGATEQRWVARLASYDFEIKKRIMSSYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YEY0</accession>
    <name>A0A9Q0YEY0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_38723</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YEY0"/>
    <dbReference type="OrthoDB" id="427129at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8021493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="105" mass="11893" checksum="BF296B370C08ED1F" modified="2023-09-13" version="1">MLGNLSEDKKGVWKTYVAPLVHAYNWTRNDSTSFSPFNLMFGYHVRLPIDLFLGRDCFENNGGGGRTHYEYADSLRNGLGYAYELASTKANTKSRAIRIAMHGRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YF16</accession>
    <name>A0A9Q0YF16_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Nose resistant to fluoxetine protein 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_39849</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YF16"/>
    <dbReference type="OrthoDB" id="207378at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002656">
        <property type="entry name" value="Acyl_transf_3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052728">
        <property type="entry name" value="O2_lipid_transport_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11161">
        <property type="entry name" value="O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11161:SF0">
        <property type="entry name" value="O-ACYLTRANSFERASE LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01757">
        <property type="entry name" value="Acyl_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="211"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="249"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="283"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="314"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="343"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="386"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="422"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="465"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="498"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="530"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="domain" description="Acyltransferase 3" evidence="3">
        <location>
            <begin position="160"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8020291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="578" mass="65269" checksum="9B231641AB4FBD84" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YGL2</accession>
    <name>A0A9Q0YGL2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">P2X purinoreceptor 7 intracellular domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_43782</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000453">
        <property type="protein sequence ID" value="KAJ8018301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YGL2"/>
    <dbReference type="OrthoDB" id="9898867at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046815">
        <property type="entry name" value="P2RX7_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36981:SF1">
        <property type="entry name" value="P2X PURINORECEPTOR 7 INTRACELLULAR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36981">
        <property type="entry name" value="ZGC:195170"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20478">
        <property type="entry name" value="P2RX7_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="83"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="P2X purinoreceptor 7 intracellular" evidence="2">
        <location>
            <begin position="171"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8018301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="233" mass="24878" checksum="A55880D0D307164E" modified="2023-09-13" version="1">MPRCFLSAVAFGRGVAFGRGVAFGRGVAFGRGVAFGRGVAFGRGVAFGRGVAFGRGVAFGLSVAFGRGVAFGLSVVFGRGVAFGRGVAFGLGVAFGLGVAFGRGVAFGRGVVFGTERRWPLHSTRRQTVGGEPELEDERVGNTAWCQCGLCEAMETRVESICCTEIGNLEKRQYRHVAYKMFTQWGHGRMGKGNRTPLPSCAVYQIRLKFPEPDADYTGFSYADEVLADAMPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YHF1</accession>
    <name>A0A9Q0YHF1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Kinesin-like protein unc-104</fullName>
            <ecNumber evidence="18">5.6.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">HOLleu_39559</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="25">Nanhai2018</strain>
            <tissue evidence="25">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>ATP + H2O + a kinesin associated with a microtubule at position (n) = ADP + phosphate a kinesin associated with a microtubule at position (n+1, toward the plus end).</text>
            <dbReference type="EC" id="5.6.1.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Secretory vesicle membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="16">Synapse</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="25">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.1.3" evidence="18"/>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8022149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030658">
        <property type="term" value="C:transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008574">
        <property type="term" value="F:plus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010970">
        <property type="term" value="P:transport along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd22705">
        <property type="entry name" value="FHA_KIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01365">
        <property type="entry name" value="KISc_KIF1A_KIF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01233">
        <property type="entry name" value="PH_KIFIA_KIFIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000023">
        <property type="entry name" value="Kinesin family member 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.20:FF:000001">
        <property type="entry name" value="Kinesin family member 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000004">
        <property type="entry name" value="Kinesin-like protein isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2520">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022164">
        <property type="entry name" value="Kinesin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022140">
        <property type="entry name" value="Kinesin-like_KIF1-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032405">
        <property type="entry name" value="Kinesin_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049780">
        <property type="entry name" value="PH_KIFIA_KIFIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47117:SF10">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47117">
        <property type="entry name" value="STAR-RELATED LIPID TRANSFER PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12473">
        <property type="entry name" value="DUF3694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12423">
        <property type="entry name" value="KIF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16183">
        <property type="entry name" value="Kinesin_assoc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 20" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="14" id="KW-0413">Isomerase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0493">Microtubule</keyword>
    <keyword evidence="12 19" id="KW-0505">Motor protein</keyword>
    <keyword evidence="7 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0770">Synapse</keyword>
    <feature type="domain" description="Kinesin motor" evidence="24">
        <location>
            <begin position="3"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="FHA" evidence="23">
        <location>
            <begin position="496"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="22">
        <location>
            <begin position="1567"/>
            <end position="1665"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="576"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1334"/>
            <end position="1357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1431"/>
            <end position="1450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1489"/>
            <end position="1523"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="20">
        <location>
            <begin position="366"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="20">
        <location>
            <begin position="620"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="1336"/>
            <end position="1347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="1500"/>
            <end position="1518"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <begin position="95"/>
            <end position="102"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00050273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00066390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="KAJ8022149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1681" mass="190834" checksum="3EC723296EEBC03B" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YIH1</accession>
    <name>A0A9Q0YIH1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Protein ZGRF1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_38233</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8023137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6513042at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000326">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052800">
        <property type="entry name" value="DNA_Repair_Helicase_ZGRF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018838">
        <property type="entry name" value="ZGRF1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010666">
        <property type="entry name" value="Znf_GRF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28535:SF1">
        <property type="entry name" value="PROTEIN ZGRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28535">
        <property type="entry name" value="ZINC FINGER GRF-TYPE CONTAINING 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10382">
        <property type="entry name" value="ZGRF1-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06839">
        <property type="entry name" value="Zn_ribbon_GRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51999">
        <property type="entry name" value="ZF_GRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GRF-type" evidence="10">
        <location>
            <begin position="2236"/>
            <end position="2278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="93"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="161"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="209"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="586"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="829"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1752"/>
            <end position="1771"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1892"/>
            <end position="1912"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1941"/>
            <end position="1970"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="106"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="172"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="210"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="235"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="253"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="283"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="833"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1754"/>
            <end position="1770"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="1943"/>
            <end position="1952"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8023137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="3088" mass="341577" checksum="D397DE0573E4E727" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YIS2</accession>
    <name>A0A9Q0YIS2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Carbohydrate sulfotransferase 15</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_39566</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8022155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YIS2"/>
    <dbReference type="OrthoDB" id="8068875at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050659">
        <property type="term" value="F:N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019319">
        <property type="term" value="P:hexose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052654">
        <property type="entry name" value="CS_Sulfotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15723">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15723:SF0">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sulfotransferase" evidence="1">
        <location>
            <begin position="350"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8022155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="534" mass="62936" checksum="CAF4C5C5ACA81204" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YIS3</accession>
    <name>A0A9Q0YIS3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_38495</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YIS3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8021329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="248" mass="27892" checksum="7FE4840AB322984A" modified="2023-09-13" version="1">MNIIMLDLKMKAPQLYVPKKPVFPDLLNNDQHVVYVDFGLSSSKDFIDDSSISSSGVESGVIIPFLSDSPNGPPKYSIAMAELYSRDRVEPAKPNIMYPFISIDEMTVEDNEAFWRNGDEELHVSFVKSNPCQTPSAATKEVFVKFFTGLSLLGWSEPDLEMCNRHPTKNYYCSVMNTIKDDVEEALPNVCQTYDIADVVECAASFDIIEVTPGFREFLLINDNAIDIIAAERFYCNATTTFKFPCVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YK30</accession>
    <name>A0A9Q0YK30_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_37814</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Elbow/Noc family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YK30"/>
    <dbReference type="OrthoDB" id="10054079at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051520">
        <property type="entry name" value="Elbow/Noc_ZnFinger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12522:SF4">
        <property type="entry name" value="ZINC FINGER PROTEIN ELBOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12522">
        <property type="entry name" value="ZINC-FINGER PROTEIN NOLZ1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="356"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="41"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="165"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="49"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="78"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="124"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="141"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="166"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8022815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="488" mass="51244" checksum="F88643201482F8F3" modified="2023-09-13" version="1">MLAARSQPIMHTEYLQPLDPLPTTLDAKKSPLALLAQTCSSIGKPDLPHNTTDAKTPSTPQDSRCGKSSPEKGTTAISDEKSYDPFKSKRDELSIAKSYASMSKDSHRTSPTSHADSKPIASATAFTSRSVSPTKSRSEHSSMSSTTVSATDTITSHSTGLSLGCSSLHSDSTHQSSKELKSHSVPIQSSHAPTILPAAHLTSPGLAYYKPGQPLFGSSHSCGCSSQLSSHFSHLDSAALASAKSDSNPLVAPGYARVKTADGATTLMPICRDPYCNNCQASQHAVASASSGCTQCRHDSYPLPGAVPLAFPLPGAPFPACPIPTSIASSPSAGLTPYLYPPFSTLPAPAQTEHGHVCNWVSGNTNCGKRFASSEELLQHLRTHTQQNSENSSSYPASDLAASLNAQLSAYYMYYPTAAPLAGSTLPGFPKPTLPSALSPNSALRYYPYKAPLSTPAITALPGLPAAYYSPYALHDMKSRLASAALPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YL04</accession>
    <name>A0A9Q0YL04_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_36953</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8024264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YL04"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8024264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="79" mass="8934" checksum="F9766D8578636D6B" modified="2023-09-13" version="1">MPYVHHNIIIEVVNRPPSGDATCFNNCMYNILSKVSEEVKFGCVTGDFDIDLRENISSDFINVIKSSGFLRTITQPIRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YL68</accession>
    <name>A0A9Q0YL68_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">RNA-binding protein 19</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_35731</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YL68"/>
    <dbReference type="OrthoDB" id="439639at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="66"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="66"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="89"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8023325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="155" mass="17328" checksum="938256E7BF6432F0" modified="2023-09-13" version="1">MTSEKLRGIFAAKGDITDLQLKYTKSGVFRRFAFIGFQSNAAARSAIDFFHNTFIDASRIQVEEAKDLTASEKPRPWSKYSDGSSAFKKKSDVKGGEKSKQKKDPGNSKAKESKKETTQTLGLDLGEVSQKVFELTHSVFLRLKLSKVINVEMLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YLN1</accession>
    <name>A0A9Q0YLN1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Corepressor interacting with RBPJ 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_34723</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YLN1"/>
    <dbReference type="OrthoDB" id="6253837at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040014">
        <property type="entry name" value="CIR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019339">
        <property type="entry name" value="CIR_N_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13151">
        <property type="entry name" value="CBF1 INTERACTING COREPRESSOR CIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13151:SF2">
        <property type="entry name" value="COREPRESSOR INTERACTING WITH RBPJ 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10197">
        <property type="entry name" value="Cir_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01083">
        <property type="entry name" value="Cir_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBF1-interacting co-repressor CIR N-terminal" evidence="2">
        <location>
            <begin position="13"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="75"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="151"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="166"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="195"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="220"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="232"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="245"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="264"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="295"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="316"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="352"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8024727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="385" mass="46367" checksum="C1534A2705A2CC67" modified="2023-09-13" version="1">MGKSYANFMCKKFFHPASYDNIKRVWMAQQRTDAEKARQEGLRSEYEREQEIYENRQLMGDEKVKHGLTFIYEPPKGFKTEEQQKDKDGEPEYKFEWQRGAPREAYAKNMEITDQPFGIAVRNQIIPSDDEEGIPEAAFLKSLSTKEKKKLLKKLNEMEKPNGLRKSTKKNKKAKQKHDSSDESDDSSTDEERRKKEKKKKTKKHQSSDATDTSEDERKQKRHVKRKKIKKEKSEKKIKKYSESDTSESSDSSEEEKKPKIRNGQRDSDRKERDRRNDYDSYEERRKDRSHRHLNGNERHRERSPVGGARQKHASHRYEVKSRDEKRERYHRRDEKKEYKKKYESMESSSEEDARKERRSKRDRDEKSHDDRRRDRSRDRGGRHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YM81</accession>
    <name>A0A9Q0YM81_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">G-protein coupled receptor 54</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_35117</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8025028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YM81"/>
    <dbReference type="OrthoDB" id="2132067at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000405">
        <property type="entry name" value="Galanin_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45695:SF23">
        <property type="entry name" value="GALANIN-LIKE G-PROTEIN COUPLED RECEPTOR NPR-9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45695">
        <property type="entry name" value="LEUCOKININ RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00663">
        <property type="entry name" value="GALANINR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 11 14" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="46"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="80"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="117"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="158"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="208"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="261"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="301"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="57"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ8025028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="380" mass="43615" checksum="1D17FFBBD65A9504" modified="2023-09-13" version="1">MAHFAVNGSLKMPTVTVGTSEPADYSTSSYSDYEGDSLEAKVVPSVLLVILIVGWVGNSVVVFVILRAGHGRVKTATNCYIVNLAITDLFFLLCCVPWTAAIFAWNDWKFGRFMCKFVFFMMHVTAQATCLTLTAMSVDRYQAIVRPLRSLQNRSTKIVTVIIIIIWTFSLVTGVPVFIYQDIVEHPYYPRQLVCHEDWPIQVMYPSYGLYCLIMMYLIPLAIISICYLRLLITLWEQVMPGLDGAHLQHRVKDMRQRRHVTWMVLAVVLAFAICWLPLYICNIWQRLFQDTYPRTNAMYIFKVTGNVLAYSNSCINPFIYSFMGENFRRYLRRACPLCFKNRGRQFVVGNSRRTGTTVMDDHPMVEFKPGSTVSESDPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YQ61</accession>
    <name>A0A9Q0YQ61_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_31274</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YQ61"/>
    <dbReference type="OrthoDB" id="5807119at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051860">
        <property type="entry name" value="Plasmodium_CSP_Invasion"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44826:SF5">
        <property type="entry name" value="DYNEIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44826">
        <property type="entry name" value="SPORE COAT PROTEIN SP85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8026454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="431" mass="47963" checksum="D0EBFFCC0D87272C" modified="2023-09-13" version="1">MRSSTYPFFHSSTHPLIQSFTHPHIHSSTQPSTHPFIHSSTHALIHSFSHSTIFSSTHSLIHSSTHSLLHSSTLPFIHSCTHLLIHTCTHSLIQSFTHFFIHPFTPALILSSTHSIILSSTLPPIHSCTHPFFHPFTHSLIHSSTHSLSHSPTLPPIHSFSHPLFHPFTQSLTHSSTHSLILSSTLPPIHSVTHPLFHPFTHSLIHSSTHSLLHSSFLPPIHSFSHPLFHPFTPALILPSDHSLLHSSFLPPIHSSTLPLIHSCTHPLFHSFTHSLIHSSTHSLIHSSTLPPIHSCTHPFFHPFTHSLIHSSTHSLLHSSFLPTIHSCTHPFFHPFTHPLFHSFTPALIHSSTHSLIHSSTLPPIHSFTHPLFHPFTPALILSSTHSLIHSSTHSILHSSTLPPIHSSTLPLIHSCTRSLVHSSTHPVIHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YQF0</accession>
    <name>A0A9Q0YQF0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_35793</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YQF0"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69989">
        <property type="entry name" value="C-terminal domain of PLC-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8023371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="200" mass="22705" checksum="FB416D762983D8D3" modified="2023-09-13" version="1">MKNPIAIRKYTDANHPTPQVICTTTSRTGLTIIAAQDETSQKTLLKDWKPIEGLRPMARLPKQKTARPETNDGIIEDIQRKQDETIAKTEECHQLQLEALRLQHQATIEKIEETNSQLFQQTSEDTIAQLNQLKGQITHFLGDVLTCLIPEKGDTPPSDYRKATVIKEKAWTHLGLDIDISRTKEELRKKSPRGQKTSSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1B8N0</accession>
    <name>A0A9Q1B8N0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP-dependent DNA helicase</fullName>
            <ecNumber evidence="1">5.6.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_44504</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.3" evidence="1"/>
    <dbReference type="EMBL" id="JAIZAY010000939">
        <property type="protein sequence ID" value="KAJ8017841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1B8N0"/>
    <dbReference type="OrthoDB" id="10071389at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010285">
        <property type="entry name" value="DNA_helicase_pif1-like_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051055">
        <property type="entry name" value="PIF1_helicase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47642">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47642:SF5">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05970">
        <property type="entry name" value="PIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0227">DNA damage</keyword>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="1" id="KW-0234">DNA repair</keyword>
    <keyword evidence="1 3" id="KW-0347">Helicase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA helicase Pif1-like DEAD-box helicase" evidence="2">
        <location>
            <begin position="2"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8017841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="99" mass="10921" checksum="4E4DF78FBC0F00B2" modified="2023-09-13" version="1">MAPTGKAAYGIKGTTIHCALQIPANQGLSNYKALTADKLNSLQVKYHNLKIIFIDDISMVGHRMFRYIDQRLQQIMGSKKVFGGVSIIAVGDLFQIKPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-04-02" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1B8X9</accession>
    <name>A0A9Q1B8X9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_43753</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000442">
        <property type="protein sequence ID" value="KAJ8018321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="3598281at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09487">
        <property type="entry name" value="SAM_superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31025">
        <property type="entry name" value="SI:CH211-196P9.1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31025:SF9">
        <property type="entry name" value="SI:DKEY-286J15.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="82"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="134"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="871"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="875"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="890"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="907"/>
            <end position="918"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8018321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1325" mass="149984" checksum="0B6C5164D8A40154" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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    </protein>
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    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
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            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2121618at2759"/>
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        <property type="component" value="Chromosome 22"/>
    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
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            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="330"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="807"/>
            <end position="918"/>
        </location>
    </feature>
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            <begin position="998"/>
            <end position="1038"/>
        </location>
    </feature>
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            <begin position="1069"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
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            <begin position="1153"/>
            <end position="1187"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
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            <begin position="1245"/>
            <end position="1272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="37"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="184"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="290"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
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            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
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            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
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            <end position="422"/>
        </location>
    </feature>
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            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
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        </location>
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        </location>
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        </location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
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        </location>
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        </location>
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    <feature type="compositionally biased region" description="Pro residues" evidence="2">
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        </location>
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        <location>
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        </location>
    </feature>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1087"/>
            <end position="1099"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1162"/>
            <end position="1177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8020496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1370" mass="158304" checksum="F3D70929930026A1" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BC79</accession>
    <name>A0A9Q1BC79_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_10053</name>
        <name evidence="1" type="ORF">HOLleu_42347</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000057">
        <property type="protein sequence ID" value="KAJ8019217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8019217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8043100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="146" mass="16108" checksum="E80448F52A892AE4" modified="2023-09-13" version="1">MGAPAVCERSCGYGCTQQHVLNRTRYSRRELRSRTSFLAHAELETGVAARKFRRCNEKSRAAARACLGKQPAFQTRTSAGPLRKRGRRELRSRTSFLAHAELETGVAARKFRRCNEKSRAAARACLGKQPAFQTRTSAGLVGGQPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BCG1</accession>
    <name>A0A9Q1BCG1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="22">Neurotrypsin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">HOLleu_37602</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="22">Nanhai2018</strain>
            <tissue evidence="22">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="match status" value="3"/>
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        <property type="match status" value="3"/>
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    <dbReference type="CDD" id="cd01099">
        <property type="entry name" value="PAN_AP_HGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Lysyl oxidase 4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Soluble scavenger receptor cysteine-rich domain-containing protein SSC5D"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.4.10">
        <property type="entry name" value="Hepatocyte Growth Factor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.20.10">
        <property type="entry name" value="Plasminogen Kringle 4"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006585">
        <property type="entry name" value="FTP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000001">
        <property type="entry name" value="Kringle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013806">
        <property type="entry name" value="Kringle-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018056">
        <property type="entry name" value="Kringle_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038178">
        <property type="entry name" value="Kringle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050127">
        <property type="entry name" value="Serine_Proteases_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24264:SF54">
        <property type="entry name" value="PEPTIDASE S1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24264">
        <property type="entry name" value="TRYPSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22633">
        <property type="entry name" value="F5_F8_type_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00051">
        <property type="entry name" value="Kringle"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00024">
        <property type="entry name" value="PAN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00530">
        <property type="entry name" value="SRCR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00018">
        <property type="entry name" value="KRINGLE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00261">
        <property type="entry name" value="LDLRECEPTOR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00258">
        <property type="entry name" value="SPERACTRCPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00607">
        <property type="entry name" value="FTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00130">
        <property type="entry name" value="KR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00473">
        <property type="entry name" value="PAN_AP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57414">
        <property type="entry name" value="Hairpin loop containing domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57440">
        <property type="entry name" value="Kringle-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00021">
        <property type="entry name" value="KRINGLE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50070">
        <property type="entry name" value="KRINGLE_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50948">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00420">
        <property type="entry name" value="SRCR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="11 16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 14" id="KW-0420">Kringle</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 17" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="9 17" id="KW-0720">Serine protease</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Kringle" evidence="18">
        <location>
            <begin position="16"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="Kringle" evidence="18">
        <location>
            <begin position="106"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="21">
        <location>
            <begin position="185"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="21">
        <location>
            <begin position="282"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="domain" description="Kringle" evidence="18">
        <location>
            <begin position="366"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="21">
        <location>
            <begin position="661"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="20">
        <location>
            <begin position="792"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="20">
        <location>
            <begin position="940"/>
            <end position="1042"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="19">
        <location>
            <begin position="1063"/>
            <end position="1298"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="61"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="123"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="646"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="757"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="769"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="863"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="899"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="906"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="918"/>
            <end position="933"/>
        </location>
    </feature>
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        <location>
            <begin position="967"/>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="980"/>
            <end position="1041"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="1011"/>
            <end position="1021"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00121"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KAJ8022641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1302" mass="145437" checksum="4F4C41B5680F1611" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BCS3</accession>
    <name>A0A9Q1BCS3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Ubiquitin-conjugating enzyme E2-17 kDa</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_37986</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BCS3"/>
    <dbReference type="OrthoDB" id="7851174at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000002">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="5">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="3">
        <location>
            <position position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8022941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="110" mass="12538" checksum="A7BFABB9487BF56D" modified="2023-09-13" version="1">MGPADSPYQGGVFFLTIHFPTDYPFKPPKVTFSTKIYHPNINSNGSICLDILRSQWSPALTVSKVLLSICSLLTDPNPDDPLVPDIARMYKTDRRKYNDLAKEWTRKYAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BCX8</accession>
    <name>A0A9Q1BCX8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_39398</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8022025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BCX8"/>
    <dbReference type="OrthoDB" id="6134204at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8022025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="221" mass="24750" checksum="C8B65FCD4C8407E6" modified="2023-09-13" version="1">MMDILGKPATIAMASTSNTALPFKRKRSSYLIHCVGNATGDLLPFKDVTKSKVISCAKEWAELNGNEGELAGEIIGKDGEVLPNVKFQEHGYHRHCYNRFTDVSKIARARAKKQNTVFEREEVAPLRASEGDDPKCVSESSDINAPKLTRSQTKTDLSYEGSHLRRPVFPAVCIICQADKYTKDGFTRKRRKEKLVECQTLTAGSLLEAAEKRHLFLIRSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BD43</accession>
    <name>A0A9Q1BD43_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_35679</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BD43"/>
    <dbReference type="OrthoDB" id="10058156at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8023301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="131" mass="15113" checksum="B8E99AA1A3B11F94" modified="2023-09-13" version="1">MLHYFQEISHLKSFTGQTVKASGKLYVDVCYGNYEGKLKLYVIDCTEYYLLGREWIGVIPLGWKSLINQRPKPEKVLMVSELEQVINKHSELFEKGLGRLTDAKAKIVLREEAQPVMRKPVKVPYALKRKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BDG1</accession>
    <name>A0A9Q1BDG1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_40212</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BDG1"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="23"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="164"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="198"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="263"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="330"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="400"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8020582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="421" mass="49795" checksum="B9C6C5F9D349735D" modified="2023-09-13" version="1">MDSQEVTLLINNSREKFKTRVPILLMIVLWSLGIIDWSPPTCESLYLYCPIIGRSRLHRKITFVVSIGWLFTMALTAYFGALTYFICFFGLKEHFLRFVIVVVCYYPSLMSGTMFYIPAFFSNRCKKSEIDEFNMSWVNLFKESAFLQRINYIKKFNMFILPRLAVYVFFNICSLAVSFVILILYIEVYNDCLTWVSYIWTASEFIGLIYLAQFCYFLYLQRVSLEEEFKESECFIKANAGKLENCTQKVKNLFCNYFQLRKLFLLWLSIIFFSITFGIAAYATWSYKKPEIRTNSTVLPTQYPLHPKGIKKYCPYSCDDPKYFENQHHLLIYNVLAFAKLLIPAVLSFSVMRGLDIKYMWNRFILRLRLMSTTDHLDFWNTFIRYTKDLHPENQLDTKLRFIIPVLGLAFGFLGGWKSHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BES7</accession>
    <name>A0A9Q1BES7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_37799</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BES7"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="18"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="50"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8022803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="76" mass="7963" checksum="DAB8767CBA549030" modified="2023-09-13" version="1">MLGGIKFSLKWTRLDSAPSPSPVAAHSGPIPIPGPPIPGQFRVQKKLDSSPKLSPSPDSGVTALVVCEPHTLQLNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BF67</accession>
    <name>A0A9Q1BF67_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_33103</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BF67"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8025521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="65" mass="7666" checksum="4AD4CA9BBD703EEE" modified="2023-09-13" version="1">MLFDMFSKCVKFSDIEPAFKCRRISIKRKIMIFLDVCEVIVSDSCFNNEVGYYNLYGIEIRIVDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BG57</accession>
    <name>A0A9Q1BG57_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_35484</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8025310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BG57"/>
    <dbReference type="OrthoDB" id="6105938at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006301">
        <property type="term" value="P:DNA damage tolerance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006513">
        <property type="term" value="P:protein monoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16449">
        <property type="entry name" value="RING-HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039577">
        <property type="entry name" value="Rad18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14134">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RAD18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14134:SF3">
        <property type="entry name" value="RING-CH-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="7">
        <location>
            <begin position="250"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="17"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="184"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="420"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="51"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="83"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="109"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="138"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="153"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8025310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="515" mass="57947" checksum="BE841B9474685AD3" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BHE7</accession>
    <name>A0A9Q1BHE7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein farnesyltransferase/geranylgeranyltransferase type-1 subunit alpha</fullName>
            <ecNumber evidence="4">2.5.1.58</ecNumber>
            <ecNumber evidence="3">2.5.1.59</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">CAAX farnesyltransferase subunit alpha</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">FTase-alpha</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Ras proteins prenyltransferase subunit alpha</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Type I protein geranyl-geranyltransferase subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_33777</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein prenyltransferase subunit alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.58" evidence="4"/>
    <dbReference type="EC" id="2.5.1.59" evidence="3"/>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8026043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BHE7"/>
    <dbReference type="OrthoDB" id="272289at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005953">
        <property type="term" value="C:CAAX-protein geranylgeranyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005965">
        <property type="term" value="C:protein farnesyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004662">
        <property type="term" value="F:CAAX-protein geranylgeranyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004660">
        <property type="term" value="F:protein farnesyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.120">
        <property type="entry name" value="Protein prenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002088">
        <property type="entry name" value="Prenyl_trans_a"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11129">
        <property type="entry name" value="PROTEIN FARNESYLTRANSFERASE ALPHA SUBUNIT/RAB GERANYLGERANYL TRANSFERASE ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11129:SF1">
        <property type="entry name" value="PROTEIN FARNESYLTRANSFERASE_GERANYLGERANYLTRANSFERASE TYPE-1 SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01239">
        <property type="entry name" value="PPTA"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48439">
        <property type="entry name" value="Protein prenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51147">
        <property type="entry name" value="PFTA"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0637">Prenyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00040965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00041392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00042436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00043086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00043219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ8026043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="325" mass="38560" checksum="A9EDBCD15447A538" modified="2023-09-13" version="1">MAADDDVESSSEEIYVFFKDRPEWKDVVPVQQDDGPHAVVRIAYSERFQDVYDYFRAVLKSNEMSERAFELTTEATELNPANYTVWHYRRELLKALNKDLAAELEYISEVIQDHPKNYQVWHHRRVLVEWMNNADKELDFTSLILRLDAKNYHAWSHRQWVIQTFNFWDGELEFVDNLLDEDLRNNSAWNQRYFVISNTTEFDDATLAHEVTYTKGFIQKAPNNESSWNYLKGILSTKDKMYDFPGLEEFCQMMYNKNIKSPYLLAFMIDLCEDKLESAESDAATLTKATELCNLLAETIDPIRRKYWNYFKRKLTMRFGQEQTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BHF0</accession>
    <name>A0A9Q1BHF0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DNA ation factor subunit beta</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_34869</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BHF0"/>
    <dbReference type="OrthoDB" id="9943677at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004520">
        <property type="term" value="F:DNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006309">
        <property type="term" value="P:apoptotic DNA fragmentation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039729">
        <property type="entry name" value="DFF40"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015311">
        <property type="entry name" value="DFF40_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044925">
        <property type="entry name" value="His-Me_finger_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13067">
        <property type="entry name" value="CASPASE-ACTIVATED DNASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13067:SF2">
        <property type="entry name" value="CASPASE-ACTIVATED DNASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09230">
        <property type="entry name" value="DFF40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54060">
        <property type="entry name" value="His-Me finger endonucleases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA fragmentation factor 40 C-terminal" evidence="1">
        <location>
            <begin position="105"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8024842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="232" mass="27449" checksum="8566CE3D4D09B49A" modified="2023-09-13" version="1">MDVTVRRVSGRPHQVKVKSFEDLKRKGSEGLKITIAMVWKMTIGNHDLNPKNYKNHLSTDKEVVFWTNLDKIMESLKVELNKSLKGNDEAHCIYNFFEMQLRGNLSDDKHDDEGWFKGLTKCDTKSEYMECKASSRIRKYYDKIADALKNINGYSDVEKVLRKFRTRLHKKKWHKALFGVTGRDADRKCDKEGKFICQGLYDKKNCPFHHTINPYRTREGRLQFQLWELDHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BHJ4</accession>
    <name>A0A9Q1BHJ4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Integrin alpha-9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HOLleu_35088</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Nanhai2018</strain>
            <tissue evidence="17">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Membrane</location>
            <topology evidence="1 13">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the integrin alpha chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8025007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BHJ4"/>
    <dbReference type="OrthoDB" id="5573735at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008305">
        <property type="term" value="C:integrin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033627">
        <property type="term" value="P:cell adhesion mediated by integrin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.930">
        <property type="entry name" value="Bicelle-embedded integrin alpha(iib) transmembrane segment"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.130">
        <property type="entry name" value="Integrin alpha, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1460">
        <property type="entry name" value="Integrin domains. Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1510">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1530">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013517">
        <property type="entry name" value="FG-GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013519">
        <property type="entry name" value="Int_alpha_beta-p"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000413">
        <property type="entry name" value="Integrin_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018184">
        <property type="entry name" value="Integrin_alpha_C_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013649">
        <property type="entry name" value="Integrin_alpha_Ig-like_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048285">
        <property type="entry name" value="Integrin_alpha_Ig-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048286">
        <property type="entry name" value="Integrin_alpha_Ig-like_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028994">
        <property type="entry name" value="Integrin_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032695">
        <property type="entry name" value="Integrin_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23220">
        <property type="entry name" value="INTEGRIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23220:SF134">
        <property type="entry name" value="INTEGRIN ALPHA-2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01839">
        <property type="entry name" value="FG-GAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08441">
        <property type="entry name" value="Integrin_A_Ig_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20805">
        <property type="entry name" value="Integrin_A_Ig_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20806">
        <property type="entry name" value="Integrin_A_Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01185">
        <property type="entry name" value="INTEGRINA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00191">
        <property type="entry name" value="Int_alpha"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69318">
        <property type="entry name" value="Integrin alpha N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69179">
        <property type="entry name" value="Integrin domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51470">
        <property type="entry name" value="FG_GAP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00242">
        <property type="entry name" value="INTEGRIN_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 13 17" id="KW-0401">Integrin</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="898"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="12">
        <location>
            <begin position="159"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="12">
        <location>
            <begin position="220"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="12">
        <location>
            <begin position="286"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin alpha first immunoglubulin-like" evidence="14">
        <location>
            <begin position="333"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin alpha second immunoglobulin-like" evidence="15">
        <location>
            <begin position="495"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin alpha third immunoglobulin-like" evidence="16">
        <location>
            <begin position="697"/>
            <end position="884"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF20805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF20806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ8025007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="961" mass="106602" checksum="EA77FA9F5929834B" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BHL7</accession>
    <name>A0A9Q1BHL7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_35208</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8025097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BHL7"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8025097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="95" mass="11274" checksum="E5093AFF9F420578" modified="2023-09-13" version="1">MFVCHLGNFHSRIVSPVRGTCTFFGDFFAGNLVVFITQKLIATYLEKNRKISLHLFLCLNEKKEKKSKKKKKKQREKEKDDKITKRITNMRRIAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BID8</accession>
    <name>A0A9Q1BID8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Carbohydrate sulfotransferase</fullName>
            <ecNumber evidence="9">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_31969</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Golgi apparatus membrane</location>
            <topology evidence="1 9">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the sulfotransferase 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="9"/>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BID8"/>
    <dbReference type="OrthoDB" id="2019940at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016051">
        <property type="term" value="P:carbohydrate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018011">
        <property type="entry name" value="Carb_sulfotrans_8-10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005331">
        <property type="entry name" value="Sulfotransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12137">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12137:SF54">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03567">
        <property type="entry name" value="Sulfotransfer_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="8 9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8026974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="417" mass="49327" checksum="A1400CF4BDB917D8" modified="2023-09-13" version="1">MSVVYMYTHSQRVSKTQPSTLEMYLRCKAWFFPSRKDIIKLIFVIGSMAVLKICLEWKHSSASKHADVSKEVNVVPLEFSSRQDPGFYKRGGHFVRAKSRPSKTKKAKLRRGSGREEAMLREISSKLSIKKNNVSREFHKNEKRNLQLGMAERCDKLHRMVQNIQTTEQLVRSLRNIFVLDEKKLLFCYVPKIACTNWKRILLILKATVDHMSIHQLADENLPILSTYKVNDALRRIKEYKKLLFVREPFSRLLSAYRDKLEKSGRVDSDRFRKDWSASMKSFSRVRDPKDDVTFLEYINYLADPLHPINQPKDEHWNPIYQLCRPCDVQYDFIGRMENLERDSEYVLKHVIDSDLKLNLHNSTNFTNSSSADIVRQYYSQIPVQTVMQLYKKYRPDFELFSYPFPWFAFNRSSFED</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BIY2</accession>
    <name>A0A9Q1BIY2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Myophilin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_32619</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the calponin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BIY2"/>
    <dbReference type="OrthoDB" id="21595at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031032">
        <property type="term" value="P:actomyosin structure organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050606">
        <property type="entry name" value="Calponin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001997">
        <property type="entry name" value="Calponin/LIMCH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000557">
        <property type="entry name" value="Calponin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003096">
        <property type="entry name" value="SM22_calponin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47385">
        <property type="entry name" value="CALPONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47385:SF14">
        <property type="entry name" value="TRANSGELIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00402">
        <property type="entry name" value="Calponin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00889">
        <property type="entry name" value="CALPONIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00888">
        <property type="entry name" value="SM22CALPONIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51122">
        <property type="entry name" value="CALPONIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="7">
        <location>
            <begin position="28"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="165"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8027468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="195" mass="20974" checksum="53805A04BD1DDB57" modified="2023-09-13" version="1">MADDVANNPNPMGMSRAVQNKIEAKRSDSLEADCRNFISHVTGEQVEGERFDGLKDGVLLCKAVNVIIPGAVPKIENASKNKFAKRANIEAFIQAMDKAGVKGAELFQVNDLYENKNIPQVCVGITALGRLAQKQPGYSGPVLGPREATENKREFTQEQLDAGKHITGLQMGSNKGATQAGMGIGRPRQVNPDGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BJF6</accession>
    <name>A0A9Q1BJF6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_32833</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BJF6"/>
    <dbReference type="OrthoDB" id="6413693at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040139549" description="Ig-like domain-containing protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8027634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="125" mass="14303" checksum="AA4221563DDB8D30" modified="2023-09-13" version="1">MALRKYFSFCLVLATYHTQVLGYNRSAVNVNQTIHVLQSSSVLLECDNLSANDKSKWKHGEDIIFIDKNALSTDLKSTVVLSQNYSLFISPTLLLHEGLYQCIRNSVTVRRYQVIVEFHQSFTSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BKA8</accession>
    <name>A0A9Q1BKA8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Allatostatin-A receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_30181</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BKA8"/>
    <dbReference type="OrthoDB" id="5950040at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00637">
        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24243">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24243:SF208">
        <property type="entry name" value="PYROKININ-1 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="34"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="70"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="107"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="154"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="220"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="271"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="311"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="10">
        <location>
            <begin position="50"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8028054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="370" mass="41937" checksum="51680EB31C10569D" modified="2023-09-13" version="1">MEGEIECNGRILNLTMVPHEGIVAGFLKPRLEEMFTYTLFAFVVVVGLFANASFIYVVAKVEKMHTVMNAYLVNLAVADIMILGYGYVDRLYFYLSTPLNVGTNFLGPMYCVVSATFEVTYYVSLFTVTLFSMERYTAVCHPMEHIKISTSGRTRMLLLGTWVFAVVIAVLQIPGKGRRDLLCLVFSRDSEFHHLSGDVPICGEIVKGYFKHVGVPLQLLPFYIALFGNAFFYIMMVGTLINRGGSIVGSEDNDKVSAAMIKSRNAATRMIVINGCVFFFCQAPIQIFITVTWIFKLVNRNILMPFAQRRNIFLVLTILLYLNSAVNPFIYSVTNRQYRIAFFQAFKIKRKHRLGKYTSPRSRKTAMSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BN61</accession>
    <name>A0A9Q1BN61_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Eukaryotic translation initiation factor 3 subunit J</fullName>
            <shortName evidence="4">eIF3j</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_28960</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the eIF-3 subunit J family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BN61"/>
    <dbReference type="OrthoDB" id="20381at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005852">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.60">
        <property type="entry name" value="Eukaryotic translation initiation factor 3 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03009">
        <property type="entry name" value="eIF3j"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023194">
        <property type="entry name" value="eIF3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013906">
        <property type="entry name" value="eIF3j"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21681">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21681:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08597">
        <property type="entry name" value="eIF3_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4 6" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3 4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="76"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="212"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="25"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="45"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="233"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8029540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="242" mass="27729" checksum="43CE4754B1EBF5F6" modified="2023-09-13" version="1">MADWDDEDFDPDAGFTKPAVSDRWEGEDEEEVKDAWDDEDEDEKKEDGESESKPEEVKAFQVKKKKIKIEEALKKKEEKRKEKLKQLREQDDEPQKELTPEEQLAEKLRQEQMQKDADLELTKEMFAETTLAEDSLDALNPTTKEQFDRFAALLKTKLGTLESSPFYVDFLEDLVKDCCINLDTDYVKRIGNSVSAIASEKAKAQKAAVKGKKKSKKGVLAGSNKAGKRDELDTYDDMEDFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BN64</accession>
    <name>A0A9Q1BN64_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">SHC-transforming protein-like 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_29345</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BN64"/>
    <dbReference type="OrthoDB" id="9938362at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01670">
        <property type="entry name" value="Death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00173">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000488">
        <property type="entry name" value="Death_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051853">
        <property type="entry name" value="SH2-Ras-GEF_adapter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14247">
        <property type="entry name" value="BREAST CANCER ANTI-ESTROGEN RESISTANCE PROTEIN 3 HOMOLOG-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14247:SF8">
        <property type="entry name" value="RAS-GEF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00531">
        <property type="entry name" value="Death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50017">
        <property type="entry name" value="DEATH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0727">SH2 domain</keyword>
    <feature type="domain" description="SH2" evidence="3">
        <location>
            <begin position="225"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="Death" evidence="4">
        <location>
            <begin position="653"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="199"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="326"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="376"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="458"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="328"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="397"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="461"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="486"/>
            <end position="532"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8029838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="730" mass="83820" checksum="B8F6B5D18C8EA8E6" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BNB3</accession>
    <name>A0A9Q1BNB3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">DNA repair protein complementing XP-A cells-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_29153</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the XPA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BNB3"/>
    <dbReference type="OrthoDB" id="68328at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000110">
        <property type="term" value="C:nucleotide-excision repair factor 1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901255">
        <property type="term" value="P:nucleotide-excision repair involved in interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000715">
        <property type="term" value="P:nucleotide-excision repair, DNA damage recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070914">
        <property type="term" value="P:UV-damage excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21076">
        <property type="entry name" value="DBD_XPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.530.10">
        <property type="entry name" value="XPA C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000465">
        <property type="entry name" value="XPA/RAD14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022656">
        <property type="entry name" value="XPA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022658">
        <property type="entry name" value="XPA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037129">
        <property type="entry name" value="XPA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022652">
        <property type="entry name" value="Znf_XPA_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00598">
        <property type="entry name" value="rad14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10142">
        <property type="entry name" value="DNA REPAIR PROTEIN COMPLEMENTING XP-A CELLS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10142:SF0">
        <property type="entry name" value="DNA REPAIR PROTEIN COMPLEMENTING XP-A CELLS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05181">
        <property type="entry name" value="XPA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01286">
        <property type="entry name" value="XPA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="Putative DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00752">
        <property type="entry name" value="XPA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00753">
        <property type="entry name" value="XPA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="XPA C-terminal" evidence="10">
        <location>
            <begin position="179"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF05181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8029694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="230" mass="25937" checksum="EEB96B5728367530" modified="2023-09-13" version="1">MVDSSGDLYEKVVGWEMSFLPLQKVVLNLNMENSAGEPASLNTESDNSMAEGSIQSSSVCLSDAQKAKIEKNRQKALALRRSREGCKPYTKKKPEKKQVKIPEGAIRLPGGLLDTNAGFLIDEEEVLEEKELSVIQEPGPLLGGDLLRCLDCGNSFQDSSLYKQFDHSVCDDCRDNEDKHNLITKTEAKNEYLLKDADLDRREPPLKFLVRKNPHNPRWGDMKLYLRLQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BQH4</accession>
    <name>A0A9Q1BQH4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Mitochondrial fission 1 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_27384</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Involved in the fragmentation of the mitochondrial network and its perinuclear clustering.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion outer membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Peroxisome membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">The C-terminus is required for mitochondrial localization, while the N-terminus is necessary for mitochondrial fission.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the FIS1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BQH4"/>
    <dbReference type="OrthoDB" id="421154at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000266">
        <property type="term" value="P:mitochondrial fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043653">
        <property type="term" value="P:mitochondrial fragmentation involved in apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016559">
        <property type="term" value="P:peroxisome fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12212">
        <property type="entry name" value="Fis1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000147">
        <property type="entry name" value="Mitochondrial fission 1 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016543">
        <property type="entry name" value="Fis1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033745">
        <property type="entry name" value="Fis1_cytosol"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028061">
        <property type="entry name" value="Fis1_TPR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028058">
        <property type="entry name" value="Fis1_TPR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13247:SF0">
        <property type="entry name" value="MITOCHONDRIAL FISSION 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13247">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 11 TPR REPEAT PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14853">
        <property type="entry name" value="Fis1_TPR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14852">
        <property type="entry name" value="Fis1_TPR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF008835">
        <property type="entry name" value="TPR_repeat_11_Fis1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="9 11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 11" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="10" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="131"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="12">
        <location>
            <begin position="79"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR008835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ8030848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="158" mass="17668" checksum="43C090527712E10C" modified="2023-09-13" version="1">MYRNETAKMEEIVNETISVEEIKKFERAYNSELARGKVNSRTQFEYAWCLVRSPYTNDIKKGTVLMEQLLQGGNPQIQRDCLFYLGVGHFRLKDYTKALQFVKGLLQIEPNNHQAKELEVVIKKKMNKDGLVGMAVIGGAAVVIGGLVAAGMAISKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BRW3</accession>
    <name>A0A9Q1BRW3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Estrogen sulfotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_24880</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sulfotransferase 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BRW3"/>
    <dbReference type="OrthoDB" id="205623at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11783">
        <property type="entry name" value="SULFOTRANSFERASE SULT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sulfotransferase" evidence="3">
        <location>
            <begin position="35"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8031630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="301" mass="34508" checksum="F2002A71FCC5A016" modified="2023-09-13" version="1">MSKLPPLHFYKGPTPLPPPVTDEAIDALKTFEYRDGDVLIASYPKCGTHWSNEVVQLILHDGDPTKLDHRHRRACLEIKDVQDFRKLDQVKPTVELVKTDPSPRVLMTHLGPSYLSEDTWKRRIPIVFIFRDPRDVVVSEYNFLKKFLGVDGQPVMKEDSFDEFLEGFISDHVGYGNWCDHALAYEKKATEGENILFVTYEDMKKDLPTVVRSIASHIGQEISDEVVAKVVANSGIEAMKRNYQEASKTQENANLHLSNFVNKGVAGQWKTDFSGDKFTKFNDTFKERVKDSVYAKRYFDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BTZ1</accession>
    <name>A0A9Q1BTZ1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_26169</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BTZ1"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8032604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="72" mass="8088" checksum="542D0B46021B8140" modified="2023-09-13" version="1">MACFPSEATDTTARHNHCCWNEGLCIGRAKEGANRKLLRADIYSSARTESRVFLHPASLTPFGRDSKNYDQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BU74</accession>
    <name>A0A9Q1BU74_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_22929</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8032860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BU74"/>
    <dbReference type="OrthoDB" id="8895157at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31025:SF27">
        <property type="entry name" value="SI:CH211-193K19.2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31025">
        <property type="entry name" value="SI:CH211-196P9.1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8032860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="188" mass="21334" checksum="C43704DBF7706EA8" modified="2023-09-13" version="1">MRITTKPLISSFMAELDKHTPKLLALMRAKGGVQGKKIKEVMKVLDENQNINMPVSTRREVILLGQCAYLNEDSTSLVKEYLDSEEDKIITGEVKRTMGIFIIRREGVTNEMPADVGITLEGTEVINNLDRLAVAVALLFGLIYALNMNYPKNPRYFFEVIQKVFMGLDEEHLTPKVQALKNKLFFVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BV21</accession>
    <name>A0A9Q1BV21_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_23343</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BV21"/>
    <dbReference type="OrthoDB" id="6103133at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032727">
        <property type="entry name" value="CLAMP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28457">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 189"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28457:SF2">
        <property type="entry name" value="SIMILAR TO 4930578I06RIK PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14769">
        <property type="entry name" value="CLAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="277"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="304"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8033185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="322" mass="36992" checksum="C501526A1596D05C" modified="2023-09-13" version="1">MHSGKFHGNACKMAASLCWPTNLVLSYEDVKNIATLNKTEGKLHLAKCLQWKSKIIEDDRFLRNTIQLEYLYDVIQFCATKGFPWSQISSVCLLAKEFLERCIGVTQTEAVNIFRDLVVQNSENIKESILKSFTTYIFSTFMHHFQLYQFVLTEAREEFLVKADVEIEPPGEILSLHTAKPKHIWDYEQKLQEITEGELLRQEELRKQKEALVGKEETTYAKKFEEKGENASLTREVLEQIIAEAAQSQCQRVEKLLQVKVEEKHEGLNFYLEKTSLPRPVELGPPPRTRQPPNHSAKSRLSTKTKASAKSTQSKTSKKAVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BYF4</accession>
    <name>A0A9Q1BYF4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cell adhesion molecule 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_22226</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BYF4"/>
    <dbReference type="OrthoDB" id="6413693at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45889">
        <property type="entry name" value="IG-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45889:SF8">
        <property type="entry name" value="IG-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040496848" evidence="2">
        <location>
            <begin position="20"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="299"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="15"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8035110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="337" mass="37173" checksum="5EDD7C3B7EA11DC3" modified="2023-09-13" version="1">MGWYLIFVTCFFSFPIARTTILVGPKSAVYPEGSNVTLQCLAERTNRIVWEDVNDNTVIFIDKEKNTEKVKFDNFVISNGEDNFSLTIVNAELSDEGFYACEEGDESRSANVTIEAGPDLSIHVLVPENRTITEDERVFVLCKAEGAKPAVRLKLKFGDNEWIESNNTITNEGLHFTTETRVAHRVTRADTTVTCQATGQVSIPPSTTMSSMNVLHKPACVIELGTDLAECQCVSNPAVSTYSWYVNGELQSTDPSLRIHEYIPANITCLASNEVGISPLTSRYVSLTDLQSDASTKDVVIIISVIAAVIVCAIVIGVILIIVYKQSKRESTFLERL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C2G8</accession>
    <name>A0A9Q1C2G8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_17633</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8036954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C2G8"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040374128" description="C-type lectin domain-containing protein" evidence="2">
        <location>
            <begin position="22"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="3">
        <location>
            <begin position="31"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="180"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="180"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8036954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="297" mass="32379" checksum="25207C6405678506" modified="2023-09-13" version="1">MSSWNQLALLLIICLDKNKLAVGLLSACFDYNGDCLQEDTVTTEHKYAEALCTSNGANLLSVSSNEMDDNDRQGVLISNSVNFPLWLDCHYSGGARRSGGMVFYGPGNPDNTGVWRWTEDLDEEDEGSCVTLLSSGEIALTDCEEQHTVICASNWSTIVDNSRTPPSTSSITTIATTLEVQTTSHPESTTTKFTTTDISTDDTTKSTSPTTTNFTTISETTTKTTVMTTANSRTDQKRNLCETSEMTTEMTSSYQTTGKSVQLNVKITSKMYSVLPKSSKMSSVKMNADVIHVKEHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C489</accession>
    <name>A0A9Q1C489_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_18792</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C489"/>
    <dbReference type="OrthoDB" id="10044788at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01671">
        <property type="entry name" value="CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01670">
        <property type="entry name" value="Death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001315">
        <property type="entry name" value="CARD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000488">
        <property type="entry name" value="Death_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00531">
        <property type="entry name" value="Death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50209">
        <property type="entry name" value="CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50017">
        <property type="entry name" value="DEATH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CARD" evidence="3">
        <location>
            <begin position="31"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="Death" evidence="2">
        <location>
            <begin position="127"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="208"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8037865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="228" mass="25838" checksum="77FB2BCBE6F277AF" modified="2023-09-13" version="1">MVQKTKSAARVSPTPAPAEGPPKPSTPGDEVTEEARKLLWMYKMALVNNLQPFEMIKHLRENMVINAERAKELRREGRGNKHATGEAVIGELMTRGKREFDVFVFGLKKQNINLLADLLEEKHESKLINILEPASKKLGHHWKRLSLELGTAGFVPKIETKYSRIREQALYSLLLWEDSSGISATERRLLDGLQALQMKWALAHLRKLSNPKSGKKKKGKKKSGKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C536</accession>
    <name>A0A9Q1C536_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein HID1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_19052</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8038076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C536"/>
    <dbReference type="OrthoDB" id="432953at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005797">
        <property type="term" value="C:Golgi medial cisterna"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000138">
        <property type="term" value="C:Golgi trans cisterna"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026705">
        <property type="entry name" value="Hid-1/Ecm30"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21575">
        <property type="entry name" value="PROTEIN HID1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21575:SF12">
        <property type="entry name" value="PROTEIN HID1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12722">
        <property type="entry name" value="Hid1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="649"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="657"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="685"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="740"/>
            <end position="754"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8038076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="869" mass="96664" checksum="42593CFE47723DCC" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C546</accession>
    <name>A0A9Q1C546_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RNA demethylase ALKBH5</fullName>
            <ecNumber evidence="4">1.14.11.53</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Alkylated DNA repair protein alkB homolog 5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Alpha-ketoglutarate-dependent dioxygenase alkB homolog 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HOLleu_19069</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Nanhai2018</strain>
            <tissue evidence="16">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>an N(6)-methyladenosine in mRNA + 2-oxoglutarate + O2 = an adenosine in mRNA + formaldehyde + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:49520"/>
            <dbReference type="Rhea" id="RHEA-COMP:12414"/>
            <dbReference type="Rhea" id="RHEA-COMP:12417"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74449"/>
            <dbReference type="EC" id="1.14.11.53"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:49521"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus speckle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the alkB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.53" evidence="4"/>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8038091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C546"/>
    <dbReference type="OrthoDB" id="271595at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990931">
        <property type="term" value="F:mRNA N6-methyladenosine dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.590:FF:000002">
        <property type="entry name" value="RNA demethylase ALKBH5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.590">
        <property type="entry name" value="Alpha-ketoglutarate-dependent dioxygenase AlkB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037151">
        <property type="entry name" value="AlkB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032860">
        <property type="entry name" value="ALKBH5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32074">
        <property type="entry name" value="RNA DEMETHYLASE ALKBH5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32074:SF2">
        <property type="entry name" value="RNA DEMETHYLASE ALKBH5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="298"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="298"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="310"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="340"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="355"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00033313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ8038091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="375" mass="43051" checksum="A9387A99A85B20C0" modified="2023-09-13" version="1">MSDKTSTKDSSPKRSSRHGIEETSSGRHSSRRRRSPSGSSNSRHEKRSHPYPGNDHKKKRRRGSSSPESERNLEEIKKINAGIRQIHDIFGESECRKIEEEIDEIVKNSKRGLYRKQTVDRAPLRSKYFFGEGYTYGAQLAKRGPGQERLYPKGHVDEIPKWVKELVIKRLEEEKIIPKDFVNSAVINDYLPGGCIVSHIDPIHIFDRPIVSISFMSDSALSFGCKFSFKPIRVSKPLVSLPLSKGCVTLLSGYAADEVTHCIRPQDVTSRRAVIILRRVFPDAPRLEEWEVKNLPQATHYKSNSNGYQSRRDSRRDSHEDSRRSSNHREERRRKRRRSSSSSSSSSSSSDDRRRRSRHQRKSSHRRHSGGHGNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C570</accession>
    <name>A0A9Q1C570_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_15761</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C570"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8038364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="111" mass="13021" checksum="94B5714B8036B232" modified="2023-09-13" version="1">MKMRYSEKMNPKVFGGGQRSCGVTRGQKEYFPRVSKIITWAYQRSISEISQEYPRLSSGVPRGQKANFARIPKIAIWGHQRSKRYISQEFPRLNRNTKTVFFQLIKTAFDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C5P1</accession>
    <name>A0A9Q1C5P1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Acyl-CoA-binding domain-containing protein 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_16187</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C5P1"/>
    <dbReference type="OrthoDB" id="4567at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000062">
        <property type="term" value="F:fatty-acyl-CoA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022408">
        <property type="entry name" value="Acyl-CoA-binding_prot_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000582">
        <property type="entry name" value="Acyl-CoA-binding_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035984">
        <property type="entry name" value="Acyl-CoA-binding_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24119">
        <property type="entry name" value="ACYL-COA-BINDING DOMAIN-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24119:SF0">
        <property type="entry name" value="ACYL-COA-BINDING DOMAIN-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00887">
        <property type="entry name" value="ACBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00689">
        <property type="entry name" value="ACOABINDINGP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47027">
        <property type="entry name" value="Acyl-CoA binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00880">
        <property type="entry name" value="ACB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51228">
        <property type="entry name" value="ACB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ACB" evidence="6">
        <location>
            <begin position="11"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="154"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="187"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00018419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8038693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="245" mass="27579" checksum="2740D6FE40D2DD37" modified="2023-09-13" version="1">MDELFENESPLQESFNNAAQHLKSLVDRLSSEDLLFFYARYKQATVGPCNSKRPGMFDFQGKQKWDAWKKLGSMESEQAMQEYVSHMDEVDDNWRDSKNSKAGLGVGVSTMYNKESEINDSDKTMFDWCKEGNVEKLQGILSSSSASLNDLDESGLALIHWACDRGCIEVIDLLARLQADMNLPDIDGQTPLHYAASCDHVSIVQRLLEHGADARRKDGENQFPADTTDNPEILEILSKTCQEVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C6K2</accession>
    <name>A0A9Q1C6K2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_17021</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C6K2"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8039340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="93" mass="10705" checksum="1079436B4CE6F321" modified="2023-09-13" version="1">MLTVTRNKMQLIHLICQDIISHKEDFKQHKILLTGSDSLLVEIDRGVIIKRHDMRTTREEGDTMLVQQVAWVTAKKVLVVAADMDIVVLLLHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C7F0</accession>
    <name>A0A9Q1C7F0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_15069</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C7F0"/>
    <dbReference type="OrthoDB" id="5988992at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46670">
        <property type="entry name" value="ENDO/EXONUCLEASE/PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46670:SF3">
        <property type="entry name" value="ENDONUCLEASE_EXONUCLEASE_PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="1">
        <location>
            <begin position="50"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8040698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="328" mass="37873" checksum="D71342BCD406323B" modified="2023-09-13" version="1">MKWRSMWDHNNHVHHDVLRYLPSETRYDSNDHCCFGLVNARSLRNKCDQFLHFVMTENLDFCVIVESWLKNTDTVEIATLKPEGYDFINVERRERVGGGIGIFFKNTIAVTLIDSGEPLPFEFARYAIKIGTFMVDIIALYRPPYSAQHPVSIYTFVSEFADFANELFLTANRCLFMGDFNIQINRDLERSTIAFVDVLNTLSLNQLVIGPTHVSGNTLDLILIRESSDFSITNVRNDFFLSDHRFILATLSTCPVLVRKRVQFRRLNSIDHESFRASLSDDMEKILKLESLDDMVTAYNYHTKKVLDDHAPVVTKLITARPRCSMVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C9C9</accession>
    <name>A0A9Q1C9C9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_14741</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C9C9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8040446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="189" mass="21244" checksum="858043BEE1E95950" modified="2023-09-13" version="1">MHMEDKIYFLKEVISSVSLRPISKDLGFRAFKSIIMDSRSEDWRESNFLWLMWNFHTACEEKNSALRGEEEIFWPMTTAVVTLENVIDRLGYQTTVFTQRSRGARSKPELGRGDNDPFLTPENVVVGSKRKHSEKESGVSAEVEADPKPTADRYSDPSKGVHFKTQRGHGVSKGVYSQPAKDGGGVAGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C9X7</accession>
    <name>A0A9Q1C9X7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_12295</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C9X7"/>
    <dbReference type="OrthoDB" id="10035002at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027867">
        <property type="entry name" value="SPATA48"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34759">
        <property type="entry name" value="SPERMATOGENESIS-ASSOCIATED PROTEIN 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34759:SF1">
        <property type="entry name" value="SPERMATOGENESIS-ASSOCIATED PROTEIN 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="213"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8041471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="351" mass="40846" checksum="CF8C47BF9478C575" modified="2023-09-13" version="1">MTSISMESDQFQPSQATNYNLSYRRSTLSLPPIKFHQIPSIALKEVPKLPPRKFPIEPIGPCRRWERLARFQGQLPNIRLFTDKPIVRELPPKTVRSYAIRKLKKGALHYPKLSRAKEEEEEEKSELECDPEDKVASDVIDDLCKRFRWTTTNERAIEGAHLPLPEIPLPSTELEETSNTLMHRPRIFEERPEPWQRIATIWDRCQTRKHVGQSLGNISSAEDQGIEKKKNQTAGKRSQRNLRPDISVIEEIYKLCEKENLTKTFVRQCPGYAGFLPRSPLKYEPMTRVPKQDLYMMSSMAAAYRSLPSCGKSRYSRRGPMSRTVTLTYPYNPYNKVENVPYFRKDMGTNW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CAF3</accession>
    <name>A0A9Q1CAF3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Bactericidal permeability-increasing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_11769</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CAF3"/>
    <dbReference type="OrthoDB" id="10255543at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.15.10.10">
        <property type="entry name" value="Bactericidal permeability-increasing protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.15.20.10">
        <property type="entry name" value="Bactericidal permeability-increasing protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017943">
        <property type="entry name" value="Bactericidal_perm-incr_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032942">
        <property type="entry name" value="BPI/LBP/Plunc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001124">
        <property type="entry name" value="Lipid-bd_serum_glycop_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017942">
        <property type="entry name" value="Lipid-bd_serum_glycop_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10504">
        <property type="entry name" value="BACTERICIDAL PERMEABILITY-INCREASING BPI PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10504:SF131">
        <property type="entry name" value="BPI2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01273">
        <property type="entry name" value="LBP_BPI_CETP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02886">
        <property type="entry name" value="LBP_BPI_CETP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00328">
        <property type="entry name" value="BPI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00329">
        <property type="entry name" value="BPI2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55394">
        <property type="entry name" value="Bactericidal permeability-increasing protein, BPI"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040420859" evidence="3">
        <location>
            <begin position="19"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="domain" description="Lipid-binding serum glycoprotein N-terminal" evidence="4">
        <location>
            <begin position="27"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="Lipid-binding serum glycoprotein C-terminal" evidence="5">
        <location>
            <begin position="268"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8041064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="503" mass="56321" checksum="9C0A3808C208DF0F" modified="2023-09-13" version="1">MSPLSVVLLGCIIAGVVGDDPPGLKARITPMGLEFASKRARSEILKMAKYHSVPDQHGDYGMWIGTVHYRAYRLYISEISIISASIQPEKGGVWVSLNKARIAVHGSWEDEFNWLPFGDTGSLDVILNRVYLRLFINIGYDGMGKPTVRLSTSSCAFRVVYISLSLKGKGKYNWIYRKMKESLEEMIRKKLYAQVCPQLVREVNIKAAEILKNIKVVRPITKYAEIDLGLTSQPEFGSSVTTYHKGTVYETAHHQEPPFNVSVLPGDPDESNMAVFWITDYAINSACYVLHNTGSFMYILTKDNIPAGVNLNLNTSGFEMKFLIPELSKLYPKRLMQIYMHTSGPPKVKFGVSNVSVAMEGYISVSVVEPNRSLTSVFTLGATANISARLGSYMTNVTWDKVSISFDVKLLKSAISDLKIERLRQKLKLALKMFIMPKIKQMGNAGIPIPSLPLQGYALSNLNISHGQNFLKIGTDMYHIDWLSDIFLKGRSQPIPTFSKLFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CDQ9</accession>
    <name>A0A9Q1CDQ9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 18">Pre-mRNA-processing factor 19</fullName>
            <ecNumber evidence="5 18">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">HOLleu_09657</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">Nanhai2018</strain>
            <tissue evidence="20">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Ubiquitin-protein ligase which is mainly involved pre-mRNA splicing and DNA repair. Required for pre-mRNA splicing as component of the spliceosome.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 22">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 18">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="18">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 18">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 18">Belongs to the WD repeat PRP19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5 18"/>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8042800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CDQ9"/>
    <dbReference type="OrthoDB" id="687049at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071006">
        <property type="term" value="C:U2-type catalytic step 1 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070534">
        <property type="term" value="P:protein K63-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16656">
        <property type="entry name" value="RING-Ubox_PRP19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000043">
        <property type="entry name" value="pre-mRNA-processing factor 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000027">
        <property type="entry name" value="Pre-mRNA-processing factor 19, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038959">
        <property type="entry name" value="Prp19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013915">
        <property type="entry name" value="Prp19_cc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055340">
        <property type="entry name" value="RING-Ubox_PRP19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43995">
        <property type="entry name" value="PRE-MRNA-PROCESSING FACTOR 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43995:SF1">
        <property type="entry name" value="PRE-MRNA-PROCESSING FACTOR 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08606">
        <property type="entry name" value="Prp19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04564">
        <property type="entry name" value="U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24814">
        <property type="entry name" value="WD40_Prp19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00504">
        <property type="entry name" value="Ubox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51698">
        <property type="entry name" value="U_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 18" id="KW-0227">DNA damage</keyword>
    <keyword evidence="15 18" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8 18" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="14 18" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="16 18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="10 18" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="9 18" id="KW-0808">Transferase</keyword>
    <keyword evidence="13 18" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7 17" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="U-box" evidence="19">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="17">
        <location>
            <begin position="264"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="17">
        <location>
            <begin position="350"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="17">
        <location>
            <begin position="392"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="17">
        <location>
            <begin position="475"/>
            <end position="508"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00015618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU367101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAJ8042800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="508" mass="55322" checksum="1BEB1892777F7852" modified="2023-09-13" version="1">MALVCSISHEVPEHPVVSPSSNHVFEKRLIEKYIAENGVDPISQEPLTVEQLIDIKVSPLVKPRPPTATSIPAILKILQDEWDACMLHSFTLRQQLQTARQELSHALYQHDAACRVVARLTKEVTAAREALATLKPHAAPGMAPIPALPPGAAAQPQVQEPMEAAEAGPAGMTDEIIQKLQEKANLLTAERKKRGKNVPKELLPSDDIRVFKPKSSHPGLHSASNPGILSLDLQAADTNKVLTGGADKNAVVFNKETEQVTAILKGHTKKVTNVIYHPTEDVVFTSSPDTSIRIWIVANASCTQTINAHDAPVTGLSLHATGDYLLSCSEDKHWAFSDIATGRVLTKVADESVSSALTCAQFHPDGLIFGTGTDDSIIKIWNLKERTNVANFPGHSGPITALAFSENGYYLATAADDAVVKLWDLRKLKNFKNISLDDRYEVKSLTFDQSGTYLAVAGSDVRVYLCKQWQDLCVFSDHTDIATGVRFGNHASFLASTGMDRNLKFYGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CFH1</accession>
    <name>A0A9Q1CFH1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Lectin 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_11645</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8044242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CFH1"/>
    <dbReference type="OrthoDB" id="418245at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050111">
        <property type="entry name" value="C-type_lectin/snaclec_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22803">
        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040294105" evidence="1">
        <location>
            <begin position="23"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="2">
        <location>
            <begin position="34"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8044242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="181" mass="20005" checksum="19A962EC59E16D43" modified="2023-09-13" version="1">MQSQTIFLLGVLLVMLVGGSEACQIPCPPLWTGFKGKCYRLFHDKLLFADAEDACKALKLIDCNGDVLTTGHLASIHSEEEQQFLVSLVTSSLPLETDDPTEWDPQVFIGMVVGSSNSVQYWADESSFDYNGWFPGEPNNGPNSRGAIAAGTHSEGLWADVYNTARLRYICQLPCIKFRHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CGN7</accession>
    <name>A0A9Q1CGN7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small ribosomal subunit protein uS12m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_07889</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uS12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CGN7"/>
    <dbReference type="OrthoDB" id="361013at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03368">
        <property type="entry name" value="Ribosomal_S12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000115">
        <property type="entry name" value="28S ribosomal protein S12, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006032">
        <property type="entry name" value="Ribosomal_uS12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005679">
        <property type="entry name" value="Ribosomal_uS12_bac"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00981">
        <property type="entry name" value="rpsL_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11652">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S12 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00164">
        <property type="entry name" value="Ribosom_S12_S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01034">
        <property type="entry name" value="RIBOSOMALS12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00055">
        <property type="entry name" value="RIBOSOMAL_S12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8044977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="167" mass="19073" checksum="43E0DADFD7DB04CD" modified="2023-09-13" version="1">MMWQLFGRPASTLPSLCKFCQQYVSSKGYSVLKTLSGSTFTNPSSPVFQQLRTITVNQLHRRGPFRKERKFNPKKTHPMDHRPQMKGVVIRTLIRKPKKPNSANRKCAKVRLSNGKEVTAFIPGEGHNLQEHNIVLVEGGRTQDLIGVKHRIIRGKYDCSHVVKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CH21</accession>
    <name>A0A9Q1CH21_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Histone H1-gamma, late</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_07246</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the histone H1/H5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CH21"/>
    <dbReference type="OrthoDB" id="10070184at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00073">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005819">
        <property type="entry name" value="H1/H5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005818">
        <property type="entry name" value="Histone_H1/H5_H15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00538">
        <property type="entry name" value="Linker_histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00624">
        <property type="entry name" value="HISTONEH5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00526">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51504">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0158">Chromosome</keyword>
    <keyword evidence="1 2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="H15" evidence="4">
        <location>
            <begin position="13"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="95"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="95"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="113"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8044484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="191" mass="20153" checksum="0CD9484F068688F6" modified="2023-09-13" version="1">MATQQKKKAAKPAGPKTIDLIKKAIIALKEPKGSSTAAIKKQLASMGVDVAKKNTFINKALKKGVADGVLKQVKGTGASGTFKLDTAKAVAAEKAKQKRAKTAERRKAAQAKARAKRAAAKAKRAAKKAAAKKKKTAKKAAAKKKKATKPKKAVKPKKKAAKKPAKKLAVKKPAAKKPAKKVQKKKKPAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CIT4</accession>
    <name>A0A9Q1CIT4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Alpha-1A adrenergic receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_08755</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CIT4"/>
    <dbReference type="OrthoDB" id="9874984at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00637">
        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45698:SF1">
        <property type="entry name" value="TRACE AMINE-ASSOCIATED RECEPTOR 13C-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45698">
        <property type="entry name" value="TRACE AMINE-ASSOCIATED RECEPTOR 19N-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 8" id="KW-0675">Receptor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="30"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="63"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="110"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="148"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="186"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="297"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="330"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="7">
        <location>
            <begin position="42"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8045700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="377" mass="42845" checksum="131E0B8D7B9B0B33" modified="2023-09-13" version="1">MELLYTNTPSYSVNPLVQTGGVSVEDVFQILYLTFGILGLFGNGLVLLVMVRVRSLRTITNFFITNQSIIDFVTSIFVLIFKYNPTVIDFSNQGYNFWTIFVCKFWHSKYIFWAMMGASTNNLIVITIERWMAVVFPIVYRNRVTRKTASICAVLPWILGFMFHLHKPASRHFEDGVCFSSFGNRAIQAVVGIFLFVVKLLLPVFIMVIVYTSILKKLKPILTNQKVKNLSRSTTMLNTDTNLSLVSKLAIDSNVQTSNDTVVDLGKFSSANTVTNFTSHSKTSQQSKIRMNILKTLFLVTILFVICWTPISIVFLIYNLGGKINPTSTIYNLFVLLAYVNIWINPVVYTFKYRKFQEGLRKTFGFTGKIKAKVSAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CM95</accession>
    <name>A0A9Q1CM95_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_06310</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CM95"/>
    <dbReference type="OrthoDB" id="5334309at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017868">
        <property type="entry name" value="Filamin/ABP280_repeat-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50194">
        <property type="entry name" value="FILAMIN_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="Filamin" evidence="1">
        <location>
            <begin position="85"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8047333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="602" mass="68599" checksum="2FDC3E6D288BF35A" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CM99</accession>
    <name>A0A9Q1CM99_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Non-structural maintenance of chromosomes element 3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_06948</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CM99"/>
    <dbReference type="OrthoDB" id="205198at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1210:FF:000001">
        <property type="entry name" value="melanoma-associated antigen D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1200">
        <property type="entry name" value="MAGE homology domain, winged helix WH1 motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1210">
        <property type="entry name" value="MAGE homology domain, winged helix WH2 motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037445">
        <property type="entry name" value="MAGE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041898">
        <property type="entry name" value="MAGE_WH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041899">
        <property type="entry name" value="MAGE_WH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002190">
        <property type="entry name" value="MHD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11736">
        <property type="entry name" value="MELANOMA-ASSOCIATED ANTIGEN MAGE ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11736:SF14">
        <property type="entry name" value="NSE3 HOMOLOG, SMC5-SMC6 COMPLEX COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01454">
        <property type="entry name" value="MAGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01373">
        <property type="entry name" value="MAGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50838">
        <property type="entry name" value="MAGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MAGE" evidence="2">
        <location>
            <begin position="40"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="23"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8047838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="266" mass="30973" checksum="BE069801EDDA6579" modified="2023-09-13" version="1">MPRTANNRRNVSEDEDDEDYTEASQSSQARQAAANDAQIMERKVGELVQYFLIMEQNKIPIKRGDISRNVLKDYKSIFQEVLMKAKESLSNIFGFEMVEVEHSSAKTKTKMYFLLNKLDSELREDLVEISSENPKNALLWIVLAIIFMNESVISETQLWQALQKLGLPQDEKNHPVFGDLKRLITVEFPRQLYLECTRVPSTDPASFEFRWGERATAEVDKEKLLHMVTEIYGNNMTPQSWRTQYREVQLWRQEKGLADEPMEQDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CMW4</accession>
    <name>A0A9Q1CMW4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_00398</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CMW4"/>
    <dbReference type="OrthoDB" id="6630012at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8048191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="240" mass="27958" checksum="03CB3D2889376111" modified="2023-09-13" version="1">MGKPENAVRPVPLVVKKESETRWSARVEAVKPFNKYLREILQVLEKMIDSENETTETRSDAKQLYHCMLSYDFLTLLEFWDIILIGIDRVQKKLKDQTMNFDDAPMDSKALISMVRGKVLISESVEEGLNLCQEWKVEVKRRRGRKKRMPSENARDAGLTAKEEMERVMKGILDHVNREMDERFQRLHDTDSKFGFLLYVHGLCCGTIYSSSPDEPKDNRRRGLGNVDIFVSMITRKQIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CN31</accession>
    <name>A0A9Q1CN31_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NACHT, LRR and PYD domains-containing protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_00515</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CN31"/>
    <dbReference type="OrthoDB" id="427518at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312">
        <property type="entry name" value="NACHT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312:SF2">
        <property type="entry name" value="NUCLEOTIDE-BINDING OLIGOMERIZATION DOMAIN-CONTAINING PROTEIN 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NACHT" evidence="2">
        <location>
            <begin position="202"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8048266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="813" mass="94001" checksum="35E0CFFF5AD008E8" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CNK4</accession>
    <name>A0A9Q1CNK4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_06899</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CNK4"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8047799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="178" mass="19371" checksum="6B08F7283CC64503" modified="2023-09-13" version="1">MTTLAIAKLVQEVQEKLHVCTTDQLEAVSGDVSLPEASLIKAKAKGRLGLLGLLNRHLSSEELEESSDQGRAKIEELKEALDKVVHEEEATPSPGENEDVSPQHPIVSRIPANHQYIHRDFKIPGQITATQHKDGLGFTSLIHQIEAGLRKGYPEVEVVEDVIRATTLGERSSVASSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CNN8</accession>
    <name>A0A9Q1CNN8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sulfotransferase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_00236</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CNN8"/>
    <dbReference type="OrthoDB" id="416710at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48312">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48312:SF1">
        <property type="entry name" value="SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19798">
        <property type="entry name" value="Sulfotransfer_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8048068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="311" mass="35834" checksum="AC6EFBD4CCCF7B02" modified="2023-09-13" version="1">MAANGTSSTPNRVFMWINPRSLSTSFEKCVGFMDGAQIWHEPYTTCYFNLLFNDPETKKLFPGMELFAKKFYEAQKLIDAGGHLYQGGNLTSSKKFNYDWVKAQLENPLEDGKKFMFMKDAAFSIDGHYDKLPEQVDVKHTFIIRHPNRVALSVRRLLSQLVGFEGKVEDFDIGSGHPFMAWEKLSPDPMYKLWNYVRENIDPNPIVIDADDLQTDPEQILRKYCEAVGIPFKKKYLKWEKSDESLKYFYGALDQMVWGKNEGCYDAAFLSSSFQPLSGPVPATIPDNCVAYAEEFREGYKVMYETRIKLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CP40</accession>
    <name>A0A9Q1CP40_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">YqaJ viral recombinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_01264</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CP40"/>
    <dbReference type="OrthoDB" id="10063170at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd22343">
        <property type="entry name" value="PDDEXK_lambda_exonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051703">
        <property type="entry name" value="NF-kappa-B_Signaling_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011604">
        <property type="entry name" value="PDDEXK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019080">
        <property type="entry name" value="YqaJ_viral_recombinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46609">
        <property type="entry name" value="EXONUCLEASE, PHAGE-TYPE/RECB, C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46609:SF8">
        <property type="entry name" value="YQAJ VIRAL RECOMBINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09588">
        <property type="entry name" value="YqaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YqaJ viral recombinase" evidence="1">
        <location>
            <begin position="67"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8048798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="235" mass="26307" checksum="562534D2F674271C" modified="2023-09-13" version="1">MGSCIHRYEHHILQLIAQLNYFVEFYQATQSFTDLVSGCLSGEPTLSTADVNQTEIETRGQSNNPTWFAQRKDRITTSKVHAVVTRVRTIKASETEVDTSRLVKLISGGSVPNPNLPALKYGREMEGQAKKLYVDVMRAFSHPSVSVAECGLYVDAEHIYLAATPDGIVTCKCCGTGLLEIKCPLSSAHMIPSDECVQYLIEDRSGHLRLKRTHAYFFQVQKQLGVAKKNWCDFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CQ96</accession>
    <name>A0A9Q1CQ96_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_01301</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CQ96"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8048830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="232" mass="27045" checksum="D035A57EBCC1483E" modified="2023-09-13" version="1">MPFAYFVRYWGGLSFRRFFPSLFSNGEPHSWRPTGAYSQMRDTLRIMSTVGDVSQSSARDLYRAVFDKLVGQIQSSNCASGPDVWRVVHSRSLDYRRRDLLWRIAHDAIVTNLKRLRWRLGDGVCPRIRCTKWESVPHVFWQCDYVALFWEWFQVLTDRLTFCNTWAVSQDYALYGLSPPPCNASVQGILTFVSASIKLALWRDRGGMVEMVNFFQRGDEAECLGFPRYKRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CQG1</accession>
    <name>A0A9Q1CQG1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Sulfotransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_01816</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CQG1"/>
    <dbReference type="OrthoDB" id="10019582at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053259">
        <property type="entry name" value="Golvesin-related_Golgi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32301">
        <property type="entry name" value="COUNTIN RECEPTOR CNR3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32301:SF6">
        <property type="entry name" value="GOLVESIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8049191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="395" mass="45527" checksum="F255AB0C22094A01" modified="2023-09-13" version="1">MSGHYVASGSGSWSFQGIFACLVAILTVVVYSTYFGYSSNRTSVNVRLMKPNFAKTSSHRSVERKTTKVPATPPQNECNIVHEKLNSCNKLPNFFLYEDQRHHFQNAIINFWHLQKASGSTVASCLVDLYKRLGLPLTAHGGSSCRKKEEMLGYANYFNSSNGQPVVRGHDTLGLCDFFKEKITDRKCSVFTVFRDPYDRLVSHYFFNRQVARQTSSGDQLALILNKTIKEWIRKSGSPVWRAFSNKWKFERKDNGKEICTELQRPGAMTIEERWPPIDEHLTDTVNNLDKHLSFIGLTEDLQTTFQMLEEVYGLPFVEVCSDMHVLQGTYEDTNATEQRALKMSAKREIMEDPEIQHLMQFEVLVYEKAKEIFEAQKKVILKMKRARRKMRNLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CR92</accession>
    <name>A0A9Q1CR92_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_02069</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CR92"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="123"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8049355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="144" mass="16525" checksum="E32C950E9E3E64A3" modified="2023-09-13" version="1">MGNSVPREYKGAVCFRCKGTLSPTASWKDRDTQIIKGVKYRVFNKGEYYRRAFRRNEYQCRVCFFKNNGGDNDELLRSEFQRREHTSSRDACVTIAAMSDNEFSTTSCADIIRSPTLCGTECNEGNQENVETPRPIPPPYNTKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CRM6</accession>
    <name>A0A9Q1CRM6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SCAN box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_01918</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CRM6"/>
    <dbReference type="OrthoDB" id="8963689at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF13">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCAN box" evidence="2">
        <location>
            <begin position="235"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="329"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="594"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="329"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="344"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="368"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="611"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="636"/>
            <end position="656"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8049249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="656" mass="74024" checksum="445EBBBA04D98E4B" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CSG9</accession>
    <name>A0A9Q1CSG9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EamA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_03809</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CSG9"/>
    <dbReference type="OrthoDB" id="306876at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22911">
        <property type="entry name" value="ACYL-MALONYL CONDENSING ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="93"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="120"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="148"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="180"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="212"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="244"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="273"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="298"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="34"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="182"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8050561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="318" mass="35007" checksum="2C81505EBE1A6ED4" modified="2023-09-13" version="1">MFFHRSTSLGNDEEGWKKSSATCPAWIQTLFHRIGLLYGILSAITLGLQFCFARILQDKFPSDQIMFFRSIFMYVLLPQISWQLASEHDSMDLLLYILYGIFNVAGLYISYLALNFTNVGNVNAIAINLTIPTAILGNFFLGEEVGVVKFVMLFINLVGVTLIANPSVLFSKGSVTVNDFYGGCLAFVALISMTLSRITARKSALRVKMDASLMTLMSGAVGVPFCLSILMFSSWAMPSALWDWFNIASYVLLSLFANQFAMRGVEYESACNISALTSLSVPLAYIVGLFYLKDDFKVISIVGATLVVGSTFLSLLGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CTS7</accession>
    <name>A0A9Q1CTS7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Branched-chain-amino-acid aminotransferase-like protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_03576</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CTS7"/>
    <dbReference type="OrthoDB" id="416710at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48312">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48312:SF1">
        <property type="entry name" value="SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19798">
        <property type="entry name" value="Sulfotransfer_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8050389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="287" mass="32757" checksum="A76A24347A555242" modified="2023-09-13" version="1">MDGAEVWHEPYLNSFINHVNSSPELLQRYPKIDKTSREAEEASVKDGGTLQPLSVFRYDWVQEQLEAPLKKEKTFLFVKDLPLAMDGHFDKLPRVPFKHTFIIRSPFRSAPSLRKDCLDLFKYEGNIDEFNMIEGNPYTPIDLPNPNHLHAFWQYIRNTIDPNPVVIDSDDLQNFPEQILRKYCEAVGIPFKTTYLKWDAGEKPFKGINGPLRLVADGAYSFVNAVSSSCFLPITAQPPTFESLTPDEQKYCSTMFKGFYPFLPPSTDMPSLLSGDAVLYGRVLTGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CUF4</accession>
    <name>A0A9Q1CUF4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_04459</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8051039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CUF4"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34377">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT (TPR)-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34377:SF3">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT (TPR)-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="77"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="107"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="135"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="12"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8051039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="212" mass="24674" checksum="158640AC8736DAC4" modified="2023-09-13" version="1">MSKAFVVLLEYHHHHRHRHRHRHRHRHRHRHRHRHRHRHRHRHRHRHHPHHPHHPHHHPHHHPQHHPPPSPSPPPPLILLLLIIVIVIIIVIVIVILILILIVIVIVIVIVIVIVIVIVILILILILILTVTVTVTVIVIVIVIILIIITSTTIAKATTATTSEGDGGGDDDNDNKRVRLLTMAHPHCKHGSYPCFSTCDMVITRFLVFDLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1GZC2</accession>
    <name>A0A9Q1GZC2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_29232</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1GZC2"/>
    <dbReference type="OrthoDB" id="10494444at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="108"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="293"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="446"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="929"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="549"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="116"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="172"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="192"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="316"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8029757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="963" mass="106750" checksum="C2457D918C8B91B2" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H0Q5</accession>
    <name>A0A9Q1H0Q5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Sorbitol dehydrogenase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Polyol dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_28727</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H0Q5"/>
    <dbReference type="OrthoDB" id="1879366at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003939">
        <property type="term" value="F:L-iditol 2-dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006062">
        <property type="term" value="P:sorbitol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05285">
        <property type="entry name" value="sorbitol_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000068">
        <property type="entry name" value="Sorbitol dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045306">
        <property type="entry name" value="SDH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43161">
        <property type="entry name" value="SORBITOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43161:SF9">
        <property type="entry name" value="SORBITOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alcohol dehydrogenase-like N-terminal" evidence="11">
        <location>
            <begin position="30"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="Alcohol dehydrogenase-like C-terminal" evidence="10">
        <location>
            <begin position="181"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00026132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8029354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="352" mass="37964" checksum="0E1DEA7C5535564E" modified="2023-09-13" version="1">MASTNRNLSVVTTGKDDLLLEDREIPTPGRDEVQIAMQRVGICGSDIDFKKHGGIGDYVIKSPLILGHESSGIVSAVGTEVKHLKVGDRVSIEPGIPCRKCKLCKRGRYNLCPDSVVISVPPVDGLLRRYHCHPADFCFKLPDNVSSDEGALMEPLSVAIHACRRAGLSLGDKVLVLGAGPIGLLTLLTSNAMGAAKVAITDIHDNRLSVASKLGASACIKIDESDPQKMAEKIHTTLGWKPDITIECSGVESSFQMGIYATEAGGVFQMVGHGNPTRTLPYFVANTREIDIRGTQRYANIYPIALSMIANGLVDIKPLITHRFSLKESAEAFNTAETCKDNAIKVMIHCDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H355</accession>
    <name>A0A9Q1H355_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_26918</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H355"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8030481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="79" mass="9438" checksum="93D25E91B806B6EE" modified="2023-09-13" version="1">MKTSKMNYDLLFVEKAKNCTLQTVHMFNWKQMVSQKVEQASQVKKLSAEEAFSQWARQIHSKPEEEETRMIFSFHLKQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H3K0</accession>
    <name>A0A9Q1H3K0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_24465</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8034047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H3K0"/>
    <dbReference type="OrthoDB" id="10035901at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.30">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003034">
        <property type="entry name" value="SAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036361">
        <property type="entry name" value="SAP_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02037">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68906">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50800">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAP" evidence="1">
        <location>
            <begin position="11"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8034047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="241" mass="27258" checksum="F5F8223B2AE46336" modified="2023-09-13" version="1">MEESELSFEEISTWTVASLKDYLRIRGLKQTGNKTKLIALVYGASQLGVQPKPNEVEEDRKRSEQYRKVLKICGKTLPDPLSELNDNWKGEKEGVHCWPPVSYYEIAEWLLRDAPCKKNPSTLYPGSCSTQPAHQKKSPSTQGLYNAMLASCQDSTLFRYLEDDHKISTPIYPHDATFNEVLWVSKEVEVETIPNVPIPQPLPALAVGTNTISEFLDQLPEYTVEEVSQIELATRGQFDNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H434</accession>
    <name>A0A9Q1H434_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_21573</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H434"/>
    <dbReference type="OrthoDB" id="6910977at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002061">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000367">
        <property type="entry name" value="Zinc finger protein 572"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000534">
        <property type="entry name" value="zinc finger protein 674"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000110">
        <property type="entry name" value="Zinc finger protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050636">
        <property type="entry name" value="C2H2-ZF_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47772:SF4">
        <property type="entry name" value="ZFP64 ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47772">
        <property type="entry name" value="ZINC FINGER PROTEIN 200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="14"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="438"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="546"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="574"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="602"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="658"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="686"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="714"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="745"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="773"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="801"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="829"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="857"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="885"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="913"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="511"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="511"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="528"/>
            <end position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ8034647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="960" mass="110569" checksum="5C0F13D16F42BDC9" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H4C1</accession>
    <name>A0A9Q1H4C1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_25140</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H4C1"/>
    <dbReference type="OrthoDB" id="6162523at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:G protein-coupled peptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:hormone-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24372:SF77">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24372">
        <property type="entry name" value="GLYCOPROTEIN HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="69"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="115"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="147"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="8">
        <location>
            <begin position="1"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8031816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="188" mass="21113" checksum="8D29B450D1074252" modified="2023-09-13" version="1">MALTPIILSEFVEEIFGFSDICLSLPFVPVPDISKYHVTTEYEYYGAAFEIVQAGTDIKSLQWIYSQIVYIYFSATCVSAITLCYIAIFVSAIVSKIQSGRPGDNKDEIKMALRISIIVGTDLLCWLPVIITGILSNAGHEVSTDMYVWFAIFVMPINSAFNPFIYTIPAIKRKKKNEPSFIAVQREL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H5J2</accession>
    <name>A0A9Q1H5J2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_24504</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8034079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H5J2"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8034079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="149" mass="16403" checksum="C43B7075DEA5CFED" modified="2023-09-13" version="1">MSVAVHARLQSPSAYRAGPQTRSWRPRNVWWGSKKGEGKRHHDSSDEGSYDDISESDTNDSSSEPLEEDDMTDSSETDDSDAESDTEGADSSKEESAEDDAVTDNDGEDDDGSENVCCPIDTWEALAHMNKAMGVILKRELKKWTDSAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H685</accession>
    <name>A0A9Q1H685_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Fibrinogen-like protein A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_24334</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H685"/>
    <dbReference type="OrthoDB" id="7250310at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00087">
        <property type="entry name" value="FReD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050373">
        <property type="entry name" value="Fibrinogen_C-term_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040941">
        <property type="entry name" value="GGGWT_bact"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143">
        <property type="entry name" value="FIBRINOGEN/TENASCIN/ANGIOPOEITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143:SF444">
        <property type="entry name" value="PROTEIN SCABROUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00186">
        <property type="entry name" value="FBG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040352698" evidence="1">
        <location>
            <begin position="26"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="2">
        <location>
            <begin position="48"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="2">
        <location>
            <begin position="255"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8033945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="478" mass="54861" checksum="468913A3F1964C3C" modified="2023-09-13" version="1">MPHFHQLNFFALILNICWTLGSVSSQELHTHPNRASNEGNIGSSYFFYQQPQYPRDCLEARSQCSTSNQTGVHLIKPDGYPEPFEVFCDNTVDSGGWTVIYRFQNDSLFFNRDWTECKFGFGFLSQEFWIGNNELSFLTNQKTYELRIDVTSTEGSSFYVTYSKFRISDEYGEYKLTSVGEYKGTEDEINRFLCPPNSEDEVYIYSNCTRRCSCINGQFTCDDTYRCSPNAFCEERNNGSQCYCNEGYKGDGVNCTIDTPPSDCQDIYNRSRGSAESGVYKIKPTTWEGDPFDVYCNMTDDGEGWTVFQRRMDGSQDFFLYWSDYRDGFGSPDRELWLGNDKLHSLTTQRNYQLRIDFVNRYGAPYYAKYSSFSVGNANDNYRLSNGGYSGDAGNYLNYFNGQAFTTRDVDNDAWSGGNCATHSYTPCGTSCSYNSGWWFNNCNYSLNAPYGTNCLYWYGLPGSYCNAKYTEMKVRPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H6P0</accession>
    <name>A0A9Q1H6P0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">N-terminal methionine N(alpha)-acetyltransferase NatE</fullName>
            <ecNumber evidence="5">2.3.1.258</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HOLleu_22314</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Nanhai2018</strain>
            <tissue evidence="15">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>N-terminal L-methionyl-L-alanyl-[protein] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-methionyl-L-alanyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50564"/>
            <dbReference type="Rhea" id="RHEA-COMP:12726"/>
            <dbReference type="Rhea" id="RHEA-COMP:12727"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:133398"/>
            <dbReference type="ChEBI" id="CHEBI:133399"/>
            <dbReference type="EC" id="2.3.1.258"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>N-terminal L-methionyl-L-leucyl-[protein] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-methionyl-L-leucyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50520"/>
            <dbReference type="Rhea" id="RHEA-COMP:12711"/>
            <dbReference type="Rhea" id="RHEA-COMP:12712"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:133377"/>
            <dbReference type="ChEBI" id="CHEBI:133378"/>
            <dbReference type="EC" id="2.3.1.258"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>N-terminal L-methionyl-L-lysyl-[protein] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-methionyl-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50580"/>
            <dbReference type="Rhea" id="RHEA-COMP:12734"/>
            <dbReference type="Rhea" id="RHEA-COMP:12735"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:133406"/>
            <dbReference type="ChEBI" id="CHEBI:133407"/>
            <dbReference type="EC" id="2.3.1.258"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>N-terminal L-methionyl-L-phenylalanyl-[protein] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-methionyl-L-phenylalanyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50528"/>
            <dbReference type="Rhea" id="RHEA-COMP:12715"/>
            <dbReference type="Rhea" id="RHEA-COMP:12716"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:133382"/>
            <dbReference type="ChEBI" id="CHEBI:133383"/>
            <dbReference type="EC" id="2.3.1.258"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>N-terminal L-methionyl-L-seryl-[protein] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-methionyl-L-seryl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50568"/>
            <dbReference type="Rhea" id="RHEA-COMP:12728"/>
            <dbReference type="Rhea" id="RHEA-COMP:12729"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:133400"/>
            <dbReference type="ChEBI" id="CHEBI:133401"/>
            <dbReference type="EC" id="2.3.1.258"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>N-terminal L-methionyl-L-threonyl-[protein] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-methionyl-L-threonyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50576"/>
            <dbReference type="Rhea" id="RHEA-COMP:12732"/>
            <dbReference type="Rhea" id="RHEA-COMP:12733"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:133404"/>
            <dbReference type="ChEBI" id="CHEBI:133405"/>
            <dbReference type="EC" id="2.3.1.258"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>N-terminal L-methionyl-L-tyrosyl-[protein] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-methionyl-L-tyrosyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50532"/>
            <dbReference type="Rhea" id="RHEA-COMP:12717"/>
            <dbReference type="Rhea" id="RHEA-COMP:12718"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:133384"/>
            <dbReference type="ChEBI" id="CHEBI:133385"/>
            <dbReference type="EC" id="2.3.1.258"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N-terminal L-methionyl-L-valyl-[protein] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-methionyl-L-valyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50572"/>
            <dbReference type="Rhea" id="RHEA-COMP:12730"/>
            <dbReference type="Rhea" id="RHEA-COMP:12731"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:133402"/>
            <dbReference type="ChEBI" id="CHEBI:133403"/>
            <dbReference type="EC" id="2.3.1.258"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.258" evidence="5"/>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H6P0"/>
    <dbReference type="OrthoDB" id="47374at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031415">
        <property type="term" value="C:NatA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120518">
        <property type="term" value="F:protein N-terminal-methionine acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007064">
        <property type="term" value="P:mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000078">
        <property type="entry name" value="N-alpha-acetyltransferase 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051556">
        <property type="entry name" value="N-term/lysine_N-AcTrnsfr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42919">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42919:SF8">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="14">
        <location>
            <begin position="83"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00039121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ8035174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="246" mass="28118" checksum="6317A672ED5E6FC0" modified="2023-09-13" version="1">MDKATGIFASPSVAHTSIVILQEMLVAQLFIIRLRGDTLTLSGSCQVHPLVKKLHLVVCLISRQPYKSKVFHQKQQKLTWGTTELGDITPHNIKQLKILNSVVFPVSYNDKFYKDVLEVGELAKLAYYNDIVVGAVCCRIDTTPEGTRRMYIMTLGCLAPYRRLGIGTVMLKHVLDYCERDGNIDNLYLHVQVNNESAIDFYKHFGFEIIETKKQYYKRIEPAEAHVLQKTLTRKNEREAPNETSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H7W2</accession>
    <name>A0A9Q1H7W2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_19747</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8035923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H7W2"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8035923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="74" mass="8252" checksum="DA684075DF1E8CC6" modified="2023-09-13" version="1">MSLTGSHTPRNTTSVLLEDPLFRAGELSPSTCPPLLTHSSNHFYPRSAVTSRILLSSCKNCLKLRLYRCQMIVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H864</accession>
    <name>A0A9Q1H864_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">Origin recognition complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_20702</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the origin recognition complex (ORC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the ORC2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H864"/>
    <dbReference type="OrthoDB" id="20198at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005664">
        <property type="term" value="C:nuclear origin of replication recognition complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007220">
        <property type="entry name" value="ORC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056772">
        <property type="entry name" value="RecA-like_ORC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056773">
        <property type="entry name" value="WHD_ORC2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14052">
        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14052:SF0">
        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04084">
        <property type="entry name" value="RecA-like_ORC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24882">
        <property type="entry name" value="WHD_ORC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Origin recognition complex subunit 2 RecA-like" evidence="7">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="Origin recognition complex subunit 2 winged-helix" evidence="8">
        <location>
            <begin position="100"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU368084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF24882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8036659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="168" mass="19102" checksum="A8D6455F3C483DEE" modified="2023-09-13" version="1">MLSSLAEIPCIHIIASIDHINAPLIWDQGLFSSFNWLWYDVTTYQDYTEETSYENSLLVQQSGALTLSSMTHVLKSVPPNVQGVFNILVEAQLEHKDDISYSGMSFQDLYQKCREAFYVSSDLTLRAQLTEFKDHKLLTFKKGIDGAELLSIPVDASTLQEFKQQEDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H8Q6</accession>
    <name>A0A9Q1H8Q6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Kelch-like protein 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_14123</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8039957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H8Q6"/>
    <dbReference type="OrthoDB" id="6418787at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412:SF35">
        <property type="entry name" value="ACTIN-BINDING PROTEIN IPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412">
        <property type="entry name" value="KELCH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BACK" evidence="3">
        <location>
            <begin position="24"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8039957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="67" mass="7565" checksum="24F1786F1E131813" modified="2023-09-13" version="1">MFKISEVVDACCDFLKKELHPRNCIGVMELADAFSRIDLSGSAQAFCERNFIEVVKEEEFLGLPLNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H967</accession>
    <name>A0A9Q1H967_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Bifunctional polynucleotide phosphatase/kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_14790</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H967"/>
    <dbReference type="OrthoDB" id="19045at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046404">
        <property type="term" value="F:ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046403">
        <property type="term" value="F:polynucleotide 3'-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01625">
        <property type="entry name" value="HAD_PNP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000078">
        <property type="entry name" value="Bifunctional polynucleotide phosphatase/kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000737">
        <property type="entry name" value="Bifunctional polynucleotide phosphatase/kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006549">
        <property type="entry name" value="HAD-SF_hydro_IIIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013954">
        <property type="entry name" value="PNK3P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006551">
        <property type="entry name" value="Polynucleotide_phosphatase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01664">
        <property type="entry name" value="DNA-3'-Pase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01662">
        <property type="entry name" value="HAD-SF-IIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12083">
        <property type="entry name" value="BIFUNCTIONAL POLYNUCLEOTIDE PHOSPHATASE/KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12083:SF18">
        <property type="entry name" value="BIFUNCTIONAL POLYNUCLEOTIDE PHOSPHATASE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13671">
        <property type="entry name" value="AAA_33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08645">
        <property type="entry name" value="PNK3P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8040482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="470" mass="53555" checksum="647CC7CCCCD369D8" modified="2023-09-13" version="1">MNYSLQRALLSVSKTTTGSILRITEIRSLLAGGVRSKSQRKSFRTTLAMARKRAIAQQSTPNPSPSKRPRRATKKKEEAEPKLKFDLEWDFFGEKTSKGLHPLLELTGPAVKGSSKIAGFDLDYTLIRPKSGRKWPTGPKDWMFLSDAVPEKLKKIHDEGFKIIIFTNQRGMEKGYTTPKDFMAKIDDIITALDFPIQTFVATGENQYKKPGTLMWDRMVKKHNDGVTVDLEESYFVGDAAGRAKNWAPGKPKDFSCSDRMFAANIGIGFYTPEEFFDGEDKAIFEWRSVNPREVLERAKTNQLDDEYHLETKDFVIFTGPPASGKSSFYHHFLEPHGYVWVNRDTMKTQAKCIEAAKKAIAEGKSVAIDNTNPSTAARAAFIDLTKKKDYTVRCFVMDTPLEVCHHLNMVRQNQSSGKIRRIPNIGFNVYKKNYQEPTTKEGFSEIKKIPFYPVFENKNDEKLFLHWTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HAF5</accession>
    <name>A0A9Q1HAF5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_12858</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HAF5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8041922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="252" mass="30449" checksum="10AF3D1EFDA9C72A" modified="2023-09-13" version="1">MYVCMYVCMYVCMYVCMDGWMDGWMDGWMDGWMDGWMDGWMDGWMDGWMDGWMDGWMDGWMDGWMDGLMDGWMDGWMDGWMDGWMDGWMDGWMDGWMDGWMDGWMDGWMDGWMDGWMDGWMDGWMDGWMDGWMDGWMDGWMDGWMDGWMDGWMDGWMDGWMDGWMDGWMDGWMDGWMDGWMDGWMDGWMDGWMDGWMDGWMDGWMDGWMDGWMDGWMDGWMDGWMDGWMDGWMDGWMDGCVCACVCACFVSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HAM5</accession>
    <name>A0A9Q1HAM5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Indole-3-acetic acid-amido synthetase GH3.17</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_15752</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HAM5"/>
    <dbReference type="OrthoDB" id="10004661at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016881">
        <property type="term" value="F:acid-amino acid ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004993">
        <property type="entry name" value="GH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055378">
        <property type="entry name" value="GH3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055377">
        <property type="entry name" value="GH3_M"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31901">
        <property type="entry name" value="GH3 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31901:SF9">
        <property type="entry name" value="GH3 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23572">
        <property type="entry name" value="GH3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23571">
        <property type="entry name" value="GH3_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GH3 middle" evidence="1">
        <location>
            <begin position="40"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="GH3 C-terminal" evidence="2">
        <location>
            <begin position="151"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF23571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8038356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="255" mass="29696" checksum="0E49AF87E0A1A62C" modified="2023-09-13" version="1">MMMMVMVMVMMVMVMVMMMMVMMVMVMTMTMTVTVTVTNKPNPETRFIDEVELGKNYELIFTQSQGLYRYSFGDVINVSGFYHNCPRVKFLYRKATLLSLIGEKTSQPVIEESLENALYRWREAVELSHYSVAESFLLSEYLRSKRKGMETQELFYVFFLELKPKGSRDLPGDLNPQTLAKDIDKSVLDHHDEYFRPAIENQISRSKVFFVKPGSFSKLRDFVLANSTASRMQFKMPLKLRTVEMLEVLLLNALE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HCI8</accession>
    <name>A0A9Q1HCI8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_14497</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HCI8"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="74"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="101"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8040261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="131" mass="15179" checksum="D48A3EC2163B3131" modified="2023-09-13" version="1">MALDCISDLITKHAPIRCLGSLSEIFLAHPPISMAMFYGNRSFTVAAPEVWNSLPANVHSLINLNNLKKYLKTYIFNCLNWTLLLYFMLFCSLNYCVSSFRFYLFSFGVLRKLFVLFKLFILCKPFHCTVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HCX6</accession>
    <name>A0A9Q1HCX6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">RNA-binding protein 34</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_07658</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRM RBM34 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HCX6"/>
    <dbReference type="OrthoDB" id="442677at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12394">
        <property type="entry name" value="RRM1_RBM34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12395">
        <property type="entry name" value="RRM2_RBM34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034221">
        <property type="entry name" value="RBM34_RRM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4B/4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236:SF25">
        <property type="entry name" value="RNA-BINDING PROTEIN 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="131"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="234"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="15"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="55"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="63"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="98"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="110"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8044802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="326" mass="37212" checksum="435E9DE778A4896D" modified="2023-09-13" version="1">MNEEYNVGDVVKYLNKSSKKKKKGKSVEKSPLSDLFGSSSCHLNNTDFYGRYLKNAQSGKSLDVTESKKNSAEKKERSREGQKEKESDSKKDEKEGDVTSQETETSPSVKDAEQHKEEGKQSQPDATKDSRTIFVGNVPKKVEKKTLEKFFQEYGLIESIRVRSLAPSQQGISQQVVGKTKDINERRTNYNAYIVFEKEESAKNALEKNGEILEGHHLRVDLTLNDRKKVDSKLSVFIGSLPYDVREEDVREHFEDFGDLESVRLIRDSETGMGKGFGFLVFKDQSSVALALKMKEHRIQGRRIRVKAVENVKKQKIPKISFFRFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HCZ5</accession>
    <name>A0A9Q1HCZ5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="1">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_15044</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="1"/>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HCZ5"/>
    <dbReference type="OrthoDB" id="6022401at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00047">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000102">
        <property type="entry name" value="Receptor-type tyrosine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050348">
        <property type="entry name" value="Protein-Tyr_Phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134">
        <property type="entry name" value="RECEPTOR-TYPE TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134:SF449">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="5">
        <location>
            <begin position="51"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="6">
        <location>
            <begin position="222"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8040681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="333" mass="38803" checksum="6A3CBF8AF85B7CA2" modified="2023-09-13" version="1">MRSNRPWMVQTVEQYLFIYDCVLEWAQCRDTFIPSIDFVKRANMSDVAERRQKELKAIETLIPSMAELRQTKGKAENNKEKNRSEEILPQDGARVILQREDTENNYINASFMDGFIKPNEFITTQIPFKSTKQDFWEMVYEYDITTIVLLDDGTESQDDIPYWPERSEDLAYGSIRIHFEVEENEEFFVKRTFTIHHEEDKDEVQCVFHYHLSQLPSMQGSLTLLDPVCEVLKEIGAGKTKALDDGLTCIQCLDGAKVTGVFVACKICIDQMELENGTDVFQAVKSLRKVQPKMVDNERQYNLIFRILERFIEKKGSEEVQYANYRKTSTCAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HEZ7</accession>
    <name>A0A9Q1HEZ7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Solute carrier family 23 member 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_06185</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HEZ7"/>
    <dbReference type="OrthoDB" id="6105564at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006043">
        <property type="entry name" value="NCS2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11119">
        <property type="entry name" value="XANTHINE-URACIL / VITAMIN C PERMEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00860">
        <property type="entry name" value="Xan_ur_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="113"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="165"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="13"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="13"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8047232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="232" mass="25685" checksum="78D936DCBEA32741" modified="2023-09-13" version="1">MSMLSQFQKIGESMEKEEDLHDGKGASTSPQMSDLAYRIDERPPWMMTIMMAVQVLLLFCGLPIIQGPGAGFIIPAIAALSVRGECPAELTANSTQQERDRLKEEAYNRLNEVQGSVLVASIIQCLIGFTGLMGIFLRYIGPITIAVTLVMIGIGVIPLVMNLAGSHWCISIPTIALVIFFSKILPKWKIPCLGGKQLRLLEFFPVRFEHFSLFFITSFIVRARSDIASVTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HIG9</accession>
    <name>A0A9Q1HIG9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_05761</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HIG9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8046920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="66" mass="7560" checksum="F80F8269381089C5" modified="2023-09-13" version="1">MTMASSSKVEIRNDEEPSVNYNELSPSEAFDKPGVPLNTSWTFWLDICSWIHSSRCRSQSTKDLYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HIW1</accession>
    <name>A0A9Q1HIW1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_06388</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HIW1"/>
    <dbReference type="OrthoDB" id="434041at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd17943">
        <property type="entry name" value="DEADc_DDX20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 11" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="10">
        <location>
            <begin position="30"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="8">
        <location>
            <begin position="61"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="9">
        <location>
            <begin position="266"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="440"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="523"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="611"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="845"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="913"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="6">
        <location>
            <begin position="30"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="440"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="523"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="542"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="627"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="648"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="915"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="933"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="947"/>
            <end position="965"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8047400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1055" mass="118390" checksum="504CCAE57E938525" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HIW2</accession>
    <name>A0A9Q1HIW2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Galactosylceramide sulfotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_05338</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the galactose-3-O-sulfotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HIW2"/>
    <dbReference type="OrthoDB" id="514299at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001733">
        <property type="term" value="F:galactosylceramide sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009247">
        <property type="term" value="P:glycolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009729">
        <property type="entry name" value="Gal-3-0_sulfotransfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14647">
        <property type="entry name" value="GALACTOSE-3-O-SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14647:SF87">
        <property type="entry name" value="PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06990">
        <property type="entry name" value="Gal-3-0_sulfotr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8046606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="506" mass="59599" checksum="E527EA60CC95C504" modified="2023-09-13" version="1">MPQKASTESKKDTKELLRTKNDGMNDGKFQPLQVRTLDHSYVLPRQPPSKHSTKPSSLSSHADREDKRMLPAASEYSSVKGHLEMKPVFQKWRKFTGRKTNTTSDQRKNRRLLNSSRGYLSKKKNMTLKEATLKPTIALLHPVESRNANFFLGRSINVSNHRSPLNTGLRKSEGGVEIRSKRAEPCHPVHRIVFLKTHKAASTTTASIFERYGYYRNLTFAVGKNHVLSFSAKFSRKHILHFPGMRGKSYDMLVNHARFNRKEMDLAVENAIFITIVRKPEDQLDSAFGYFEMYRGMKLLFKRNPLEAFMEDPLMYYQNYSYAMRLISRNGQLFDLGFEHEFDENETQIKTYIRKLDKDFDLVLITEYYDESLILLKKLMCWSFEDILYISNGIRSSSHRFQKSQELVKKIRQWNYGDLLLYEHFNKTLWRKIVEYGPTFQNDLTYFRKLRHAVLQECVDLTKVNDFDRREEKFVLKNKSDKCACLLRDDVAYTHLIKKSMFERYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HJ44</accession>
    <name>A0A9Q1HJ44_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_01229</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HJ44"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8048779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="247" mass="28268" checksum="777DA7730B96CB8E" modified="2023-09-13" version="1">MKLSRWKTQRSLKKAHVPPSITLYQFLAHDEDCQLCVGEVWDLVAIQKYFQDGCQTPKEAADFEQLAKLCTGNVDFEKLAAERKDAGFKNVNGDVVARVEPFYFKDHTKVVGNTIRHMKCKMIVDQQSASICSVCKIHRQNLFQIKKKQAVDNPEGRKHTPNVYLELSELRDKVSDLKRNKNLTWRNLTLSQKIKQLMEQEGLQLREEENGAFGSVVASCSQELDALLSEGSSARLLWEQQKKKKGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HLR1</accession>
    <name>A0A9Q1HLR1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_04208</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HLR1"/>
    <dbReference type="OrthoDB" id="5950997at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319:SF35">
        <property type="entry name" value="OUTER MEMBRANE PROTEIN PMPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="139"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="188"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="244"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="339"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8050850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="424" mass="49441" checksum="108AEF7AA8224AE3" modified="2023-09-13" version="1">MCAEGYKGWLCSKCDQKFYPVFNSCLPCPEIEWLCTELVLICCFCAIVFVVVFRLYKKEKNLAKPKRSSFDIIISRGKLVLGFYQVVGEFFASVHEVNWATALKFLGSIVSVIELNIFRVIVRPKCFNERLQLNPKIEFMIGIMFPCALVLLSLVLYRTMKARYKYKRGLTGTSESLYIYTKRLRSNLWTSVIILLFVTYPSICTTVFQMYPKTCVTFCLDMSNTICEKRLRSDYEIECTNLEIYHILAFISTALYVVGFPVGLFLLLKNKIKYISLHGPPGPLYAETDDTGRHRQNLVDESTTYGSDHIWMEFLCENYKPQFWYWEIVEITRKITQTVLITLLGWENVVTVFLTIGMSLLYLMLNARYRPMKSTTEQLLQVFCFEFQLFYKLYITGVMKCVPRQNCYFLALTISILTFSKMIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HM79</accession>
    <name>A0A9Q1HM79_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ephrin type-A receptor 10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_04123</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HM79"/>
    <dbReference type="OrthoDB" id="551108at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050198">
        <property type="entry name" value="Non-receptor_tyrosine_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24418">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0675">Receptor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="61"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="21"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8050788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="373" mass="42817" checksum="FB450494DE1B694D" modified="2023-09-13" version="1">MNVAAELHEQNKTKLPTMDLPSLPESPNSSSYSIVSSEECIYYSETRETSACRRVLQQEDVSLITKLASGRLFNRWMGSLETENNQAKYVVVTSITDEAKQERQFHWNDFVKRVLELPLTEYTIKIEGIYITAGNLYLVQEHIYCKTLETYQTSNRKYVHSKEGTTPLYESEAMRLIMNILKGMEFIQSYGFLHPGLSTRKILLENQGTCKLYDFCLVADATSKVMCIKSQMDCSLNDLPLESLLRNEYTHESDVWSIAVVLWKILSSGSSPFGKFQISEIEKSQEVPRTWPEQAARIRNNLLFECWNKVTKLRPTISHLKSTFEEVWKSLPKDVATSQTTSENYVPMVGNVDKETEYSREILNEELMSTALG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YA24</accession>
    <name>A0A9Q0YA24_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_44811</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010001273">
        <property type="protein sequence ID" value="KAJ8017636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YA24"/>
    <dbReference type="OrthoDB" id="7687839at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8017636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="109" mass="12131" checksum="2D43E77E8C4E728B" modified="2023-09-13" version="1">MLTQLNLSSCCAYCHLSIMEGKAPKIPVKEAINFLIQFEKSGSSVDAFLEKNKQKKVIMQPYLLGLGESRKNITQYFIIIDHLALPCSAHDAVGAFDTLQKSLCVWPSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YB10</accession>
    <name>A0A9Q0YB10_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">BMP-binding endothelial regulator protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_41052</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8019447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YB10"/>
    <dbReference type="OrthoDB" id="6019304at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.200.20">
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052424">
        <property type="entry name" value="Kielin_Chordin-BMP_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001007">
        <property type="entry name" value="VWF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001846">
        <property type="entry name" value="VWF_type-D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46698">
        <property type="entry name" value="CROSSVEINLESS 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46698:SF4">
        <property type="entry name" value="CROSSVEINLESS 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00093">
        <property type="entry name" value="VWC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00094">
        <property type="entry name" value="VWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00214">
        <property type="entry name" value="VWC"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00215">
        <property type="entry name" value="VWC_out"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00216">
        <property type="entry name" value="VWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57603">
        <property type="entry name" value="FnI-like domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01208">
        <property type="entry name" value="VWFC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50184">
        <property type="entry name" value="VWFC_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51233">
        <property type="entry name" value="VWFD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040109751" evidence="4">
        <location>
            <begin position="32"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="domain" description="VWFC" evidence="5">
        <location>
            <begin position="157"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="VWFC" evidence="5">
        <location>
            <begin position="228"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="VWFC" evidence="5">
        <location>
            <begin position="288"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="VWFD" evidence="6">
        <location>
            <begin position="356"/>
            <end position="528"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8019447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="607" mass="67652" checksum="26F75A3E1A6E95DD" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YC88</accession>
    <name>A0A9Q0YC88_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">GTPase IMAP family member 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_43049</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. AIG1/Toc34/Toc159-like paraseptin GTPase family. IAN subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000194">
        <property type="protein sequence ID" value="KAJ8018776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YC88"/>
    <dbReference type="OrthoDB" id="8954335at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000840">
        <property type="entry name" value="Immune-associated nucleotide-binding protein 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006703">
        <property type="entry name" value="G_AIG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045058">
        <property type="entry name" value="GIMA/IAN/Toc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903:SF184">
        <property type="entry name" value="GTP-BINDING PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903">
        <property type="entry name" value="GTPASE, IMAP FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04548">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51720">
        <property type="entry name" value="G_AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="367"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="115"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="AIG1-type G" evidence="6">
        <location>
            <begin position="443"/>
            <end position="652"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8018776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="777" mass="87575" checksum="3344A85A0A4F5B52" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YD91</accession>
    <name>A0A9Q0YD91_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Integrase core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_43906</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000525">
        <property type="protein sequence ID" value="KAJ8018224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YD91"/>
    <dbReference type="OrthoDB" id="6747988at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058913">
        <property type="entry name" value="Integrase_dom_put"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46791:SF13">
        <property type="entry name" value="CLR5 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46791">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24764">
        <property type="entry name" value="rva_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase core" evidence="2">
        <location>
            <begin position="14"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8018224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="94" mass="11186" checksum="D3F69D81BB2B9F3D" modified="2023-09-13" version="1">MQFFLRRNGQDRHAANRSFVYGKSTSNQRIEFWWGILRKVCADFWIDLFSHLRDNEGLFDGGDLDKALIQFCFLNMIQVRYLISALVCFLQIFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YDQ8</accession>
    <name>A0A9Q0YDQ8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Transmembrane and coiled-coil domains protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_40028</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TEX28 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YDQ8"/>
    <dbReference type="OrthoDB" id="10072335at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019394">
        <property type="entry name" value="TEX28/TMCC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17613">
        <property type="entry name" value="CEREBRAL PROTEIN-11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17613:SF14">
        <property type="entry name" value="DEMENTIN, ISOFORM H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10267">
        <property type="entry name" value="Tmemb_cc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="540"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="349"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="410"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="137"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="161"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8020440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="612" mass="68723" checksum="0C0D99151DC134D4" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YF36</accession>
    <name>A0A9Q0YF36_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">U11/U12 small nuclear ribonucleoprotein 25 kDa protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_39732</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YF36"/>
    <dbReference type="OrthoDB" id="72819at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005689">
        <property type="term" value="C:U12-type spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17058">
        <property type="entry name" value="Ubl_SNRNP25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039690">
        <property type="entry name" value="SNRNP25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040610">
        <property type="entry name" value="SNRNP25_ubiquitin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006612">
        <property type="entry name" value="THAP_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14942">
        <property type="entry name" value="U11/U12 SMALL NUCLEAR RIBONUCLEOPROTEIN 25 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14942:SF0">
        <property type="entry name" value="U11_U12 SMALL NUCLEAR RIBONUCLEOPROTEIN 25 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05485">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18036">
        <property type="entry name" value="Ubiquitin_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00980">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50950">
        <property type="entry name" value="ZF_THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="THAP-type" evidence="7">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="6">
        <location>
            <begin position="258"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8020205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="353" mass="40507" checksum="F22AB04B94272847" modified="2023-09-13" version="1">MARICAVKGCSNSTVHLARWRKKKCAIHKRYFGSCYCHPPFVLHPFPTVTRNPSLRKQWIHAVNRKTRSGSTWSPSADDRICSFHFKDREPTPQNPVPTENLGCSVLPPSRKIPLKIPIIKWLGQKPPVVKEIPVPKLLPGLSASPSYHPEDDHSYFFRCQCTEGCHCLGCLHFFRIQQEKYTMASNQNTAPSSSRVKEKLTTEYNMFEKLTHEEVMTYLKGQISDIVDQDPLLNDLPNDVTPEEVNLKIALEYGQAMSVFVKKATGETLPIVVMQNATVRELKNAIKRHVTLQLQRDGNPKKISWRFVWRGYWLSYAGEKLTDDRKRLKEYGIQNRAEVSFVKRLRPKGGGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YGL8</accession>
    <name>A0A9Q0YGL8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_37159</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YGL8"/>
    <dbReference type="OrthoDB" id="5963861at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010998">
        <property type="entry name" value="Integrase_recombinase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34239">
        <property type="entry name" value="APPLE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34239:SF2">
        <property type="entry name" value="TRANSPOSABLE ELEMENT P TRANSPOSASE_THAP9 CONSERVED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47823">
        <property type="entry name" value="lambda integrase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8022303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="442" mass="49306" checksum="E1FAC7B782A79583" modified="2023-09-13" version="1">MQSPTVGLQEGIATGDSEQDESTTPLADNQQTVEVVKLGGFATRFQAAPPTGPPLSEELATGIRGLISEKLDEAAIKEIMDKHVPPENLDILEVPKVNPLIWENIPAKTKSKDLRLQKLQRPLVKGIVAVARCFPDNPTVEQQEALALLAQTNTEINMYRRELIKPDLNSRFHPLCKADVRVTKYLFGDDLGKVVKDMAEQQKAATVTKIGASKNSTTSSTRNQYGTCIKKWIEDCDEKSYDTMSTTTNIVLDFLVELKEEGLSYSAINTARRSALASFVVLNSGQAISNDPLISRFLKGIFNLHPPLPRYKDIWDVGVVLQYLSSLYPGQEISLKELSLKLCSLIALTTAQRMQTIHLLKIDQLKLCQSSASFYITGLVEQSRPGNHQITVKLQGYPQDERICVMNTLHTYLERTAPLRGSEKQLFISYRKPYLKVSKDTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YH73</accession>
    <name>A0A9Q0YH73_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_37332</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YH73"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8022435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="68" mass="7896" checksum="5CD02F2DEE1D652C" modified="2023-09-13" version="1">MSVFACGNTFVKNDFRQNGVFPLILEVKHWGQVLQNFVLLLMKKTCTLTHNQRHSTHTHDNVLTRQVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YHJ6</accession>
    <name>A0A9Q0YHJ6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Gastric triacylglycerol lipase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_37586</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YHJ6"/>
    <dbReference type="OrthoDB" id="9974421at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000021">
        <property type="entry name" value="Lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006693">
        <property type="entry name" value="AB_hydrolase_lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11005">
        <property type="entry name" value="LYSOSOMAL ACID LIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04083">
        <property type="entry name" value="Abhydro_lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040150869" evidence="6">
        <location>
            <begin position="22"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="domain" description="Partial AB-hydrolase lipase" evidence="7">
        <location>
            <begin position="108"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8022630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="480" mass="54945" checksum="100FD4CD86E5FDEE" modified="2023-09-13" version="1">MKYQIITILVMVLANISSSRAFNASNHQSTLFPCSTNPSSRYPSVDELLTLQGQEDNVLEWLAETWSHIKPVDGPTTAEEPKPPFCITRDKIADIHQSLQCLKRPLNVSELILKQGYPVEEHSVHTEDGFILGMQRIPHGRRESKADNCRRPAVILQHGLFACSSNWVSNLANQSLAFILADAGYDVWLGNVRGNTYSRLHERYKPHQKEFWYWSWDEMGKYDLPAMVDYTLKVTRHRELFYVGHSQGTTIAFAELSSKHSSLSKKIKLFVALAPVAKVANIFGVFRSIAESSLCCFAKDVLSEVHLYEFLPRTFLVNTLAGETVCAHPKTKFVCEFFFDMMGGYSCAHMNSSRLPVYVGNNPAGTSIYDVEHYCQMVLSDKFQKYNFGPEENLRRYGRLVPPLYHPKNIRVPVAFFSGGKDKLADVTDVEWIRRQLGKRLIYDKFLPSYSHIDFILAIDAQEYVYYDIIDLFKKFEPDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YHT3</accession>
    <name>A0A9Q0YHT3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Steroid 21-hydroxylase</fullName>
            <ecNumber evidence="14">1.14.14.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">21-OHase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Cytochrome P-450c21</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Cytochrome P450 21</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Cytochrome P450 XXI</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Cytochrome P450-C21</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">HOLleu_37829</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="24">Nanhai2018</strain>
            <tissue evidence="24">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="21">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 22">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.14.16" evidence="14"/>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YHT3"/>
    <dbReference type="OrthoDB" id="639466at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004508">
        <property type="term" value="F:steroid 17-alpha-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004509">
        <property type="term" value="F:steroid 21-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042446">
        <property type="term" value="P:hormone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042448">
        <property type="term" value="P:progesterone metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000049">
        <property type="entry name" value="steroid 21-hydroxylase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24289">
        <property type="entry name" value="STEROID 17-ALPHA-HYDROXYLASE/17,20 LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24289:SF20">
        <property type="entry name" value="STEROID 17-ALPHA-HYDROXYLASE_17,20 LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 21 22" id="KW-0349">Heme</keyword>
    <keyword evidence="10 21 22" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="13 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 21 22" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0492">Microsome</keyword>
    <keyword evidence="11 22" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="9 22" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="23" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="21">
        <location>
            <position position="447"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00044040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00044116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00044217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00044265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00044282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00044304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00044342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="KAJ8022828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="504" mass="57645" checksum="98881C2602E91C6B" modified="2023-09-13" version="1">MMELDKVTTPTLVVVSVSLLTALWFWSLRRPSAKFPPGPRGWPIIGNVMDFVGQGPHHLFMTLAEKYGDVFSVKIGSNWNVILNGSDTIKEAFLKYAVEFAGRPLTTSVEYITEGGKDIAFAPYSATWKLHRKLTYSAFRNLATGDNERFERLVFTMTERVGKILESKGDEAFDPKHIVSLAVYNFTAQMVFGKQYELDDPDLLEWQSLSQEGVEAIGNGLMADFFPVLRHIPTPGMKTLKNLVDRFFKLVNKELDSHKQSYNPRDEPKDFIEMLYHTRQTMIDEGEEGIDQISEVHIRQTVMDVFSAGTDTSIFTMHWAIALMVDYPEVQEKVQKEIDEVIGRERNPKMDDRGSLPYTECALYEVFRYSSIAPLALPHATTKDVTFRGYHIPKGTCVMPNIYSMHFDKKLWGDPENFRPEHFMDETGTMRQHPDGFLPFSTGRRVCLGESVAKAELFLIFTWLFQNFTFRKPAGKEGEIYSNVDKTALSPLLKTYEILVSKRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YIX9</accession>
    <name>A0A9Q0YIX9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Reverse transcriptase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_41256</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8019606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="425619at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09274">
        <property type="entry name" value="RNase_HI_RT_Ty3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000115">
        <property type="entry name" value="Polyprotein of retroviral origin, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.370:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 17.6-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000032">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 297-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon gypsy-like Protein"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
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    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
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        <property type="entry name" value="Integrase_p58-like_C"/>
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        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
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        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
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        <property type="entry name" value="RT_RNaseH"/>
    </dbReference>
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        <property type="entry name" value="SCAN_dom"/>
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    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
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    </dbReference>
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        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="RT_RNaseH"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RVT_1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="9">
        <location>
            <begin position="860"/>
            <end position="1018"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase" evidence="8">
        <location>
            <begin position="1274"/>
            <end position="1451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="97"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="329"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="593"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="693"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="344"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="368"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="593"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="637"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="662"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="709"/>
            <end position="733"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8019606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1666" mass="190335" checksum="07BB88323A2FA79E" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YJF8</accession>
    <name>A0A9Q0YJF8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Reverse transcriptase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_36205</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10000497at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09274">
        <property type="entry name" value="RNase_HI_RT_Ty3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000115">
        <property type="entry name" value="Polyprotein of retroviral origin, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000032">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 297-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon gypsy-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
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    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
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        <property type="entry name" value="Integrase_p58-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
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        <property type="entry name" value="RT_RNaseH"/>
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    </dbReference>
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        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN CBG26694"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Integrase_p58_C"/>
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    </dbReference>
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        <property type="entry name" value="RT_RNaseH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="10">
        <location>
            <begin position="838"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase" evidence="9">
        <location>
            <begin position="1252"/>
            <end position="1429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="307"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="571"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="671"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="78"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="307"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="322"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="346"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="571"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="595"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="615"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="640"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="682"/>
            <end position="703"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8023698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1644" mass="187418" checksum="B36CD76A4A6FC7ED" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YLV4</accession>
    <name>A0A9Q0YLV4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">DDB1- and CUL4-associated factor 17</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_38402</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YLV4"/>
    <dbReference type="OrthoDB" id="9971789at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080008">
        <property type="term" value="C:Cul4-RING E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031620">
        <property type="entry name" value="DCAF17"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14815">
        <property type="entry name" value="DDB1- AND CUL4-ASSOCIATED FACTOR 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14815:SF2">
        <property type="entry name" value="DDB1- AND CUL4-ASSOCIATED FACTOR 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15802">
        <property type="entry name" value="DCAF17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50960">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8021256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="518" mass="59647" checksum="0D96A26A74D9F328" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YM83</accession>
    <name>A0A9Q0YM83_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">WAP four-disulfide core domain protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_38633</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YM83"/>
    <dbReference type="OrthoDB" id="6060011at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019731">
        <property type="term" value="P:antibacterial humoral response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.75.10">
        <property type="entry name" value="Elafin-like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036645">
        <property type="entry name" value="Elafin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008197">
        <property type="entry name" value="WAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050514">
        <property type="entry name" value="WAP_four-disulfide_core"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19441:SF30">
        <property type="entry name" value="ELAFIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19441">
        <property type="entry name" value="WHEY ACDIC PROTEIN WAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00095">
        <property type="entry name" value="WAP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00003">
        <property type="entry name" value="4DISULPHCORE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00217">
        <property type="entry name" value="WAP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57256">
        <property type="entry name" value="Elafin-like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51390">
        <property type="entry name" value="WAP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="WAP" evidence="3">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="WAP" evidence="3">
        <location>
            <begin position="33"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="WAP" evidence="3">
        <location>
            <begin position="82"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="WAP" evidence="3">
        <location>
            <begin position="131"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="WAP" evidence="3">
        <location>
            <begin position="180"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8021436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="242" mass="25922" checksum="82AF7FE70D6D080A" modified="2023-09-13" version="1">MCIRECTSDNDCIEEKKCCSNGCSNVCRAPVVPVPKPGTCPVLEEGGVGSCKQECTSDDDCPDDKKCCNNGCENVCRAPVLPISKPGNCPASHEGDFYTCSQRCSLDEDCPDDKKCCLHDCGNVCSPPVIPFKRPGTCPVTEEGGDGKCEQQCSSDEECTEDKKCCSNGCGKICRAPVVPIPKPGTCLEPEEGWGWYSKPLCIHDCWSDDDCFRDDKCCSNGCGRVCKPPQGLPAGWSSSGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YMA8</accession>
    <name>A0A9Q0YMA8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_35609</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YMA8"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="254"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8023249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="283" mass="31028" checksum="56A5DF21BAC01FA7" modified="2023-09-13" version="1">MRGEATPRILRDNLSHIARERESTSIAEASRLPLAGKLRGRAPRCMRRLVGWRRSGHGRSALSSIYTSSHLISVDARIAGRSLRYCVLSESLWGPCLPRPPQVAPQCSVEIHCSVLYLVSLCSPFPTGVCTSVDSSRGSGLSNVYRQVRLLGVGPAVTRNRTVWRCSKGEQAMIRRGFAAVQPGVPSGEAGRASTASWFNFFSTECSLPVDDGQESLLFSPRSVPTSLAGTVRRSPRRLLADGFVRASPATIRWVFFFFLRRVCVCVLTSIVFDALGVFGLRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YNB3</accession>
    <name>A0A9Q0YNB3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Peroxisome proliferator-activated receptor alpha</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_33257</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the nuclear hormone receptor family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YNB3"/>
    <dbReference type="OrthoDB" id="7634782at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004879">
        <property type="term" value="F:nuclear receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009755">
        <property type="term" value="P:hormone-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019216">
        <property type="term" value="P:regulation of lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06965">
        <property type="entry name" value="NR_DBD_Ppar"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000030">
        <property type="entry name" value="Nuclear Hormone Receptor family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035500">
        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050234">
        <property type="entry name" value="Nuclear_hormone_rcpt_NR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001723">
        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001628">
        <property type="entry name" value="Znf_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24082">
        <property type="entry name" value="NUCLEAR HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24082:SF497">
        <property type="entry name" value="PEROXISOME PROLIFERATOR-ACTIVATED RECEPTOR GAMMA-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00105">
        <property type="entry name" value="zf-C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00398">
        <property type="entry name" value="STRDHORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00047">
        <property type="entry name" value="STROIDFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00430">
        <property type="entry name" value="HOLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00399">
        <property type="entry name" value="ZnF_C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48508">
        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51843">
        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00031">
        <property type="entry name" value="NUCLEAR_REC_DBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51030">
        <property type="entry name" value="NUCLEAR_REC_DBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8 10 14" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Nuclear receptor" evidence="12">
        <location>
            <begin position="57"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="NR LBD" evidence="13">
        <location>
            <begin position="166"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="140"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ8025644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="417" mass="48319" checksum="39DEDE72695151CD" modified="2023-09-13" version="1">MSDGTMDVLHTLERNFSAQLHAKDDLSISQSTVTAIKVSAVAEWSNNNKYPDHIDLPMKCRVCGDKASGFHYGVHSCEGCKGFFRRTYRMKLTYKPCPYLQTEPCKINVATRNKCQYCRFQKCLMVGMSHDASRFGRMPREERIRLLEEIREEENDATEEDKHRAELKELADRVHEAFSDIFKEKFTLLPQAKSTYRVKTEDGVKDNGIDDTKPMQELTEAKDIAKYCTESLLSVVEGLAKFAKKLEEFRKLDLNDQVCLLRDAAFEIAMIATSSRYIADAMWFPLEGVYIKKQIMHDLDLVYLKDKVKFFEKIKKLNINERELALFSVLALAAPDRDDLAEREKVEKFQEIILEALEQELRQTHANHRILLPRLLHLLVDLRQLVLEHIQQVQKVMLMDDPQYPGPPPLLKEIYGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YNC9</accession>
    <name>A0A9Q0YNC9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sepiapterin reductase</fullName>
            <ecNumber evidence="4">1.1.1.153</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_33284</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sepiapterin reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.153" evidence="4"/>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YNC9"/>
    <dbReference type="OrthoDB" id="153074at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004757">
        <property type="term" value="F:sepiapterin reductase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006729">
        <property type="term" value="P:tetrahydrobiopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000259">
        <property type="entry name" value="Sepiapterin reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051721">
        <property type="entry name" value="Biopterin_syn/organic_redct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006393">
        <property type="entry name" value="Sepiapterin_red"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01500">
        <property type="entry name" value="sepiapter_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44085">
        <property type="entry name" value="SEPIAPTERIN REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44085:SF2">
        <property type="entry name" value="SEPIAPTERIN REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0521">NADP</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8025663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="280" mass="30383" checksum="460A16C31CE8911A" modified="2023-09-13" version="1">MASETKTFYGVSTVAIVTGASKGIGRAIAIEFAKRFTEGLLLVLTGRSEKDLQETVSLIQADSSFSPSKTAIRSIYGDLANQSSLQLVFEGLFKDMDPAKYDHAVLVNNAGSLGDVSKYVRDFTLDDMEGLQDYFFLNVNCPLLLTSKFLSVFSSKNEKGPSKHTIVQITSLAGVQATKSLSMYCTGKAARDMLHKVVAAEEPHARVLNYAPGPVDTPMFHHIKDGSVDPDTASAMKKSVKIVLQPEKTASILCDFLGEDKYESGAHVDVFDIIGLPKMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YNE9</accession>
    <name>A0A9Q0YNE9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Elongator complex protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_36330</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="3">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ELP4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YNE9"/>
    <dbReference type="OrthoDB" id="289162at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033588">
        <property type="term" value="C:elongator holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008023">
        <property type="term" value="C:transcription elongation factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19494">
        <property type="entry name" value="Elp4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003211">
        <property type="entry name" value="Elongator complex protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008728">
        <property type="entry name" value="Elongator_complex_protein_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12896:SF1">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12896">
        <property type="entry name" value="PAX6 NEIGHBOR PROTEIN PAXNEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05625">
        <property type="entry name" value="PAXNEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8023789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="397" mass="44100" checksum="825D87B68BB6FB7F" modified="2023-09-13" version="1">MAATMSTASGVKTSFQRKGKAKSVQIQGTRPSLHNNLLLVSTGVPSFDFILGGGLAVGSVLLIEEDTYGHYAQILLRYFLAEGIMSGHNLFLASASQNPQKILKMLPFPVEGDKLLAGDAKNDPTTSAGVEPMKIAWRYESLPKVEGNTPSHKFGHSYDLSRTVTPELLSRVITDTFYLPEEMKEESKSSPLYLCPTHQKLLRRIESQIRDGGYHLTDSVQSKQTPERTILRIALHSLGSPLWWDENSSEGIDTLTSLCRFLHALRGLLRNSLAVAVVTMPTHLLEVFTDVRRLERLCDAAVQIESFAGSEKETNPMYEEYHGLVHVKQLPRLNSLTLSTPDTADLGFKLKRKKFTIEKLHLPPEFSDSTSRPQEDVVLPSSSTGCSSIFQKRGLDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YNX9</accession>
    <name>A0A9Q0YNX9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_34828</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YNX9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8024810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="111" mass="12854" checksum="1EF665DE7042F6E9" modified="2023-09-13" version="1">MPYNLLRRQDYRGNELMIQNGASSSSYLGKSDLPPIEKLLQEEFSEPPETQRQISDLFMNRNGEVSTSQTPENSLFEKITECVKNSFYCECEASLWQCWITGSCKNKYHTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YP64</accession>
    <name>A0A9Q0YP64_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">GTPase IMAP family member 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_36362</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. AIG1/Toc34/Toc159-like paraseptin GTPase family. IAN subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YP64"/>
    <dbReference type="OrthoDB" id="8954335at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006703">
        <property type="entry name" value="G_AIG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045058">
        <property type="entry name" value="GIMA/IAN/Toc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903:SF184">
        <property type="entry name" value="GTP-BINDING PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903">
        <property type="entry name" value="GTPASE, IMAP FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04548">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51720">
        <property type="entry name" value="G_AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIG1-type G" evidence="4">
        <location>
            <begin position="56"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8023817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="202" mass="22892" checksum="605F3D444A5F8B33" modified="2023-09-13" version="1">MDANYDQYLTKEELDICKSSPRVKKELGRSGKSRFEATQNKYCLTKNRNIALSDDKEELRVVVVGKTGVGKSATANTILGRESFEEGVGSNSVTVESKCLTAYYKGRRIKVIDTPGLFDTNQTQEQTLNEIARVIQLFDRGIHAFLYVLNMATPRFTEEDSETLKIVEVRTSSPVAISFLYQNTFGQPEYVNRENVKYFSFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YQ60</accession>
    <name>A0A9Q0YQ60_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_33377</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YQ60"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="163"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="197"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="263"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="333"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="396"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8025736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="423" mass="49189" checksum="9B049EC65555CC13" modified="2023-09-13" version="1">MESEIKPLISREKDNRKQIPLLLMLVMWCLGIVEWSTPQTHEQGSFHRPFIGRSKWRRRLVFMLSIGFLLLCVIVAFTGVLWYLFCYFGRKDHAIRFLSVGICYWPAMMSVPFFGLTAFARNNFTKHNDVQTDIAWPKVFNESVIVRQLTYLKTSKMTPLPKIWVFLFFHGFAFSVSCLIAILYVVMYKSCQSWISYVWMSAEIIALFYFALFSYYLYLQRIILEEEGRHTLSFIISKEGNIEVCIAPVKKFFCNYMQLRKVLLPWLCVVMFSSTFGLTAFLTWTYENNMESSKSSSNNNTEITPTEPPFYLGKLCTYSCNESHILAERTTHLVYSSVLLGKLIMPAVLTFLVIEGLDVKHVWDRFSLKLLLMLNTDNLEFWKCITEYSEKLHPNVTFDTLVGFVIPVLGLASGLLEGHHLKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YQQ6</accession>
    <name>A0A9Q0YQQ6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Deleted in malignant brain tumors 1 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_31776</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YQQ6"/>
    <dbReference type="OrthoDB" id="536948at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000001">
        <property type="entry name" value="Lysyl oxidase 4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000005">
        <property type="entry name" value="Neurotrypsin isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000011">
        <property type="entry name" value="Scavenger receptor class A member 5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000016">
        <property type="entry name" value="Scavenger receptor cysteine-rich protein type 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000007">
        <property type="entry name" value="Soluble scavenger receptor cysteine-rich domain-containing protein SSC5D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19331:SF465">
        <property type="entry name" value="EGG PEPTIDE SPERACT RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19331">
        <property type="entry name" value="SCAVENGER RECEPTOR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00530">
        <property type="entry name" value="SRCR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00258">
        <property type="entry name" value="SPERACTRCPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00420">
        <property type="entry name" value="SRCR_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 12" id="KW-0732">Signal</keyword>
    <keyword evidence="2 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040349738" evidence="12">
        <location>
            <begin position="27"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="809"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="13">
        <location>
            <begin position="32"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="13">
        <location>
            <begin position="145"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="13">
        <location>
            <begin position="255"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="13">
        <location>
            <begin position="365"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="13">
        <location>
            <begin position="473"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="13">
        <location>
            <begin position="585"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="13">
        <location>
            <begin position="696"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="102"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="170"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="183"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="214"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="327"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="391"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="404"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="435"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="498"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="511"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="542"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="627"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="658"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="766"/>
            <end position="776"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ8026827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="836" mass="92451" checksum="356E361A3AC831D6" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YSU5</accession>
    <name>A0A9Q0YSU5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_32075</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YSU5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="23"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8027045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="264" mass="29233" checksum="DCB634F875E6F41E" modified="2023-09-13" version="1">MGALASVHSLAKNVLFENDLEDTYEFEAKLAEMEERLSFLQENTNAKLKNFYNEILLEASGNEGTSVSVPICNVVDKVESTYVAKSDEVAHALFYTIQSVVESALGGDWKDSVSTLIGGTLEAFFGATKINTTGMTEEKRTYNVVWAHNALIRLDILVTKTELNQMAGLKKSTETFGGYIMVVSVVDMQKVKKEVFMYEFSQFIHEISKEDPEAIKLLNAQIRESKEIIESIGGIYEGFLNIGVGGNFYNAKPANAEPENAKPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BCW4</accession>
    <name>A0A9Q1BCW4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_40155</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BCW4"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040162676" evidence="2">
        <location>
            <begin position="25"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="554"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="554"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="564"/>
            <end position="576"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8020539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="849" mass="95489" checksum="6F63D4DBEC082D65" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BI78</accession>
    <name>A0A9Q1BI78_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Lymphocyte cytosolic protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_33865</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8026117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BI78"/>
    <dbReference type="OrthoDB" id="10044490at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051751">
        <property type="entry name" value="Immunoreceptor_sig_adapters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14098">
        <property type="entry name" value="SH2 DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14098:SF14">
        <property type="entry name" value="SH2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0727">SH2 domain</keyword>
    <feature type="domain" description="SH2" evidence="4">
        <location>
            <begin position="357"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="18"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="38"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="88"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="149"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="237"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="269"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="283"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8026117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="461" mass="50938" checksum="57864B9D8BD446FA" modified="2023-09-13" version="1">MGDSQTSLHDLLKQRLQEFKEQQAEEANDMYIAPDSQDNYEEFDTVPDNGEDDQLYLTPPGDDEDAPPPPPRDPLGAKNSSSVHRAPALPPRPGAKPLPSPSHMPLPPPGQKLLSSPAHKRLPSPGRSSVNSDENSGEEYVVPDPITADSEEMYLDPNESNEPDDCCEEYLDPNEVPRPEPPLPAPPIQRRDKSPKRTIPALNGRSRSSILPPKPTSKPEHPTSHLHPSKSVGKLFTSITSQFESKTSSVPKPPIPTSNNRPKPPQGGSMWQIQKNLKSQVPQPNPPRPAIPTPSQPPTPQLPSPRLPARPSDSQSSRPSEPVRPARSEPVRPPVPVSSRPRPSSTRGENDLSNCPWFHETLDRTKATAALNRHKKEGAFVVRKSDKNINFPYSLSLYYKGSIRNLQIGIRDDKKYALGKLKEGEGRFSSVKELIDYHRTHDVVLANNDGRTCLIEPVPIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BIE2</accession>
    <name>A0A9Q1BIE2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Cytochrome P450 4V2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_32010</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BIE2"/>
    <dbReference type="OrthoDB" id="1470350at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050196">
        <property type="entry name" value="Cytochrome_P450_Monoox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291:SF189">
        <property type="entry name" value="CYTOCHROME P450 4C3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="77"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8027004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="265" mass="30282" checksum="485FB1D7E252FDBC" modified="2023-09-13" version="1">MTMDVTKVVASFVFIGTVGLVIFLPKVSQYFLQKYRLRNFKGPRGLPILGNVLQFGRDSVEFTKFIRLNAVTFRENGSFLVYFGLYPVVVVYNAVDMEKILRNIKHDGKGYFYKTCVSPWLGNGLLLSEGSKWATRRKLLTPSFHFSILKKFLTVFNEQAQCLTEKISQLADKSSVNFPPLISLCSLDIMSETIMGLRLAAQEGGSSEYVEAVHRMSTIIVERARKPWCWNNFIFCRTQLGKAHEKYLRILHNTTKQGRNPSQSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BKR1</accession>
    <name>A0A9Q1BKR1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_30623</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BKR1"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8028403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="102" mass="11713" checksum="28DE26B5CFAE3F35" modified="2023-09-13" version="1">MVLCITISVCFNFPGEQQNGDFKENSKSCHILCKTNAHVQILLRNCNETWVDRGYTILFLSLLIPPILEFILRPSKTFKADNFSIHRIYSVTSVAHFKSVHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BNW8</accession>
    <name>A0A9Q1BNW8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Fibrillin-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_29663</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8030085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000005">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000023">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000038">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000003">
        <property type="entry name" value="fibrillin-1 isoform X1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000002">
        <property type="entry name" value="Latent-transforming growth factor beta-binding protein 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000014">
        <property type="entry name" value="Latent-transforming growth factor beta-binding protein 3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000017">
        <property type="entry name" value="latent-transforming growth factor beta-binding protein 4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000119">
        <property type="entry name" value="vitamin K-dependent protein S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000240">
        <property type="entry name" value="Vitamin K-dependent protein S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="44"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.290.10">
        <property type="entry name" value="TGF-beta binding (TB) domain"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026823">
        <property type="entry name" value="cEGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006150">
        <property type="entry name" value="Cys_repeat_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013032">
        <property type="entry name" value="EGF-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017878">
        <property type="entry name" value="TB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036773">
        <property type="entry name" value="TB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052080">
        <property type="entry name" value="vWF_C/EGF_Fibrillin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47333:SF4">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47333">
        <property type="entry name" value="VON WILLEBRAND FACTOR C AND EGF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12662">
        <property type="entry name" value="cEGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="39"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12661">
        <property type="entry name" value="hEGF"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00683">
        <property type="entry name" value="TB"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036312">
        <property type="entry name" value="Fibrillin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="45"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="42"/>
    </dbReference>
    <dbReference type="SMART" id="SM00289">
        <property type="entry name" value="WR1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57581">
        <property type="entry name" value="TB module/8-cys domain"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="38"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="25"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="34"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51364">
        <property type="entry name" value="TB"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 9" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040204696" evidence="11">
        <location>
            <begin position="24"/>
            <end position="3075"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="146"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="TB" evidence="13">
        <location>
            <begin position="184"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="246"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="288"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="TB" evidence="13">
        <location>
            <begin position="604"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="713"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="754"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="796"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="836"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="domain" description="TB" evidence="13">
        <location>
            <begin position="878"/>
            <end position="934"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="946"/>
            <end position="987"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="988"/>
            <end position="1025"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="1030"/>
            <end position="1066"/>
        </location>
    </feature>
    <feature type="domain" description="TB" evidence="13">
        <location>
            <begin position="1075"/>
            <end position="1115"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="1133"/>
            <end position="1170"/>
        </location>
    </feature>
    <feature type="domain" description="TB" evidence="13">
        <location>
            <begin position="1179"/>
            <end position="1232"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="1256"/>
            <end position="1290"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="1297"/>
            <end position="1339"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="1382"/>
            <end position="1423"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="1507"/>
            <end position="1544"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="1549"/>
            <end position="1589"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="1590"/>
            <end position="1630"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="1631"/>
            <end position="1672"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="1673"/>
            <end position="1714"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="1715"/>
            <end position="1756"/>
        </location>
    </feature>
    <feature type="domain" description="TB" evidence="13">
        <location>
            <begin position="1761"/>
            <end position="1819"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
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            <end position="1917"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="1918"/>
            <end position="1955"/>
        </location>
    </feature>
    <feature type="domain" description="TB" evidence="13">
        <location>
            <begin position="1966"/>
            <end position="2023"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
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            <end position="2079"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="2080"/>
            <end position="2118"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="2165"/>
            <end position="2205"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="2206"/>
            <end position="2243"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="2294"/>
            <end position="2331"/>
        </location>
    </feature>
    <feature type="domain" description="TB" evidence="13">
        <location>
            <begin position="2340"/>
            <end position="2391"/>
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    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="2408"/>
            <end position="2449"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="2450"/>
            <end position="2491"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="2492"/>
            <end position="2532"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="2575"/>
            <end position="2616"/>
        </location>
    </feature>
    <feature type="domain" description="TB" evidence="13">
        <location>
            <begin position="2621"/>
            <end position="2675"/>
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    <feature type="domain" description="EGF-like" evidence="12">
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            <end position="2723"/>
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    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="2724"/>
            <end position="2762"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="2763"/>
            <end position="2805"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1827"/>
            <end position="1874"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="1836"/>
            <end position="1869"/>
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    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="150"/>
            <end position="160"/>
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    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="168"/>
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    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="840"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="1034"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ8030085.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="3075" mass="332870" checksum="B2DACF840E9DDEE9" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BP37</accession>
    <name>A0A9Q1BP37_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_29642</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8030066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BP37"/>
    <dbReference type="OrthoDB" id="6496131at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33198">
        <property type="entry name" value="ANK_REP_REGION DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33198:SF19">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040106453" evidence="1">
        <location>
            <begin position="28"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8030066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="238" mass="27171" checksum="9E674EC0666033A7" modified="2023-09-13" version="1">MKAQDILLLATIVFTFNMATTTFKVEAYDPNVEIWESYEERLVQFFVANDVREDSKNVAILLSSVGPKGYGLLKNLVTLSKPSSMNLADNCKHLKEYYNPKPPVLLERFRFYNQRQGTSEGISEFLAELKRLSSSCNFGSFLNDALRDRFVCGVFDTNIQKRLLSEDDSLTLEKAVKVALSMESAAKKKKKKKNSYYKGEFSVTVYSQHVVEGHKQESPVFLLRKTRSCKKQLSYEGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BP65</accession>
    <name>A0A9Q1BP65_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_26613</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BP65"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8030251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="71" mass="7739" checksum="580C6BFF958F9E61" modified="2023-09-13" version="1">MKPLQPPHALEKSSTMEEGLGRGSLSLRKSNRTFQTPFSLTSMNSVVINNASSFTSLPLFLSIPGWGRSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BT35</accession>
    <name>A0A9Q1BT35_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Carboxylic ester hydrolase</fullName>
            <ecNumber evidence="6">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_25493</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the type-B carboxylesterase/lipase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="6"/>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BT35"/>
    <dbReference type="OrthoDB" id="19653at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003990">
        <property type="term" value="F:acetylcholinesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006581">
        <property type="term" value="P:acetylcholine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019695">
        <property type="term" value="P:choline metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050654">
        <property type="entry name" value="AChE-related_enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019826">
        <property type="entry name" value="Carboxylesterase_B_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019819">
        <property type="entry name" value="Carboxylesterase_B_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000997">
        <property type="entry name" value="Cholinesterase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43918">
        <property type="entry name" value="ACETYLCHOLINESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43918:SF4">
        <property type="entry name" value="CARBOXYLIC ESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00878">
        <property type="entry name" value="CHOLNESTRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00122">
        <property type="entry name" value="CARBOXYLESTERASE_B_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00941">
        <property type="entry name" value="CARBOXYLESTERASE_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0719">Serine esterase</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040529265" description="Carboxylic ester hydrolase" evidence="6">
        <location>
            <begin position="26"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="domain" description="Carboxylesterase type B" evidence="7">
        <location>
            <begin position="28"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="5">
        <location>
            <position position="223"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="355"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR600997-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8032074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="609" mass="67606" checksum="D7721F7B5B4C6A50" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BUE8</accession>
    <name>A0A9Q1BUE8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_23061</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8032962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BUE8"/>
    <dbReference type="OrthoDB" id="9996999at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053134">
        <property type="entry name" value="RNA-dir_DNA_polymerase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24559:SF435">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24559">
        <property type="entry name" value="TRANSPOSON TY3-I GAG-POL POLYPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8032962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="248" mass="27479" checksum="3AB170048FD1399D" modified="2023-09-13" version="1">MAEQGLRDRIKDLGSMGIVEPRAEFMEKKDILVGRTLVDPNRQILPVRIANLTSVSQTLYKGTHVGTISPVDGIVEEQGGPMEATNIDLHPDTSELFDRSSEGLTEGEKGVLFELLGDFRNVFCCEGEPLCKTGVVKHVIDTGNAHPVRQAPRCLPYHHKLQAQIEVENMLEHGVISPSKSAWASPVVLVRKKDGTIRFCMDYRKLNDLTIKDAFPLPRIDDSLDALSGAKWFSVFDLRVLAGRGRSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BUT6</accession>
    <name>A0A9Q1BUT6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_23389</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BUT6"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8033221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="97" mass="11053" checksum="D21901BB4F3C3B6D" modified="2023-09-13" version="1">MLSPIWSRTPTVFLFCFLYVLDHIFFRSIFTIPLSASGLSGLQVAASARLATCRANPGAIPGTQNLEVAYRMILEEIFFHFLVVYQRIELFQLSYSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BWG3</accession>
    <name>A0A9Q1BWG3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_20818</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BWG3"/>
    <dbReference type="OrthoDB" id="5376140at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33480">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33480:SF1">
        <property type="entry name" value="TYR RECOMBINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8034112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="379" mass="43508" checksum="039250A0573E9CAC" modified="2023-09-13" version="1">MYNLYWSITNPKCYLVHDDGPDNVSSDDDCEDTNEPFVVTSQSGAYRKWDKYSYCLFCDVRQTKLLRHCRTIHPKEIEVARALALEEDNQKETADKIWTKSRNLSNHNHNKDVFAGKYFDLVVKKRPAKGKKVRIQKCVPCLDCFGYYHCKTLWSHRKICPLRSKTSPKKARHVKEGRALLPTAGNISKPLKVVLDRMTPDDITLIAKADVDIVNVGNKVFEESNSSHRGDLDAIKKNGTDVQSYKELAKVTASQIVFFNRRGPREVESFTIEEYLKQVREGHEIQDEVQLSMTTAEKIAMGRLTLLMVRGKQGRGVPVLLTKDVRESMDILIDFNQKHNSHQMFVFSRVSEVATTPLRAHGCMTDWLNKRTDLSWLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BX45</accession>
    <name>A0A9Q1BX45_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_21341</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BX45"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8034481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="63" mass="7394" checksum="2AF97575A0FDF609" modified="2023-09-13" version="1">MPHIERKNPIVFGGGQRSPGVTGGQTLKTLLTLYLEIGSLDEFHTLYVDALWYEEEPYHFWWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BX80</accession>
    <name>A0A9Q1BX80_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">LINE-1 retrotransposable element ORF1 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_21114</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BX80"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1820">
        <property type="entry name" value="L1 transposable element, RRM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004244">
        <property type="entry name" value="Transposase_22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11505">
        <property type="entry name" value="L1 TRANSPOSABLE ELEMENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="84"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8034328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="269" mass="31346" checksum="27B1B0E977FE622F" modified="2023-09-13" version="1">MKRSRGRPKGSFGKKKREAMEKAQATKPTVFKPRVLTEECEEAILDEETSFLFDEAEEENLSEKLERTIDKAISTLSADLKGLKRDLKEELKSHVRRISKLEKENEELKKRGEALEEKVIKLEKENQEQFALLNKQERFSRRNNVRLVGFAATEHEDCVQIAKSTFAEIGLQDCKIERAHRDGKVVQGRDRHILIKVAFYQDKLFLLRNARNKLQAKPFFLTDDLTLQDLKEKKKWTTQVTALYQAGTKLRFSGGVWRMADGRPYKFKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BYL4</accession>
    <name>A0A9Q1BYL4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_22345</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BYL4"/>
    <dbReference type="OrthoDB" id="6106100at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007157">
        <property type="term" value="P:heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051427">
        <property type="entry name" value="Nectin/Nectin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23277:SF108">
        <property type="entry name" value="FASCICLIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23277">
        <property type="entry name" value="NECTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="152"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="46"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8035200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="203" mass="22759" checksum="14D978E71BA21DF6" modified="2023-09-13" version="1">MKNGLVRYCSNGLEILASRYIHGENLTCLVGNKHISASQVVNVLYPSTVKISTKSSLTIYDNDKGVSITCEANGNPQPQVLLQKKDKKGQWNVLEIGPMKSIKEKNITYTFYFGNASDDIKGMYRCIAFNDVGEAAMSETVEVEYYNSKKNVSTILLILGLLIITVLVLIKVIKTSKEDKSCYRYQIQGRKNEKKKTTVVRFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BZE1</accession>
    <name>A0A9Q1BZE1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">CLIP-associating protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_19318</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8035590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="46159at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045180">
        <property type="term" value="C:basal cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005881">
        <property type="term" value="C:cytoplasmic microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
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        <property type="entry name" value="Leucine-rich Repeat Variant"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="CLIP-ASSOCIATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21040">
        <property type="entry name" value="CEP104-like_TOG"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="4"/>
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        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="TOG" evidence="7">
        <location>
            <begin position="1"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="5">
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            <begin position="164"/>
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    </feature>
    <feature type="domain" description="TOG" evidence="7">
        <location>
            <begin position="814"/>
            <end position="1040"/>
        </location>
    </feature>
    <feature type="domain" description="TOG" evidence="7">
        <location>
            <begin position="1236"/>
            <end position="1469"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="5">
        <location>
            <begin position="1407"/>
            <end position="1443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="264"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="551"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="627"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="795"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1050"/>
            <end position="1095"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1131"/>
            <end position="1158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1176"/>
            <end position="1212"/>
        </location>
    </feature>
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        <location>
            <begin position="1465"/>
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        </location>
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        <location>
            <begin position="289"/>
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        <location>
            <begin position="555"/>
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        <location>
            <begin position="574"/>
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        </location>
    </feature>
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        <location>
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        <location>
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        <location>
            <begin position="1468"/>
            <end position="1487"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
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        <source>
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        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
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    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM01349"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAJ8035590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1487" mass="164365" checksum="2AF1FEAB657923AF" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C094</accession>
    <name>A0A9Q1C094_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DAZ-associated protein 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Deleted in azoospermia-associated protein 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Proline-rich transcript in brain protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_19662</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">In unstressed cells, promotes SIAH1-mediated polyubiquitination and degradation of the serine/threonine-protein kinase HIPK2, probably by acting as a loading factor that potentiates complex formation between HIPK2 and ubiquitin ligase SIAH1. In response to DNA damage, localizes to the nucleus following phosphorylation by HIPK2 and modulates the expression of a subset of TP53/p53 target genes by binding to TP53 at target gene promoters. This limits the expression of a number of cell death-mediating TP53 target genes, reducing DNA damage-induced cell death. Enhances the binding of transcription factor TCF7L2/TCF4, a Wnt signaling pathway effector, to the promoters of target genes. Plays a role in stress granule formation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Stress granule</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus speckle</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8035855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome 9"/>
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        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022730">
        <property type="entry name" value="DAZ_assoc-2"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DAZ-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11029">
        <property type="entry name" value="DAZAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00014066"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00045449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8035855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="81" mass="8187" checksum="B443FCB0A69E64B5" modified="2023-09-13" version="1">MYSPPQQQLPPGGPGTTYVIHGGFDAGARFDGNAPPSIPPPPPGIAPNAAQMAAASGANVHITQQQGDWMTGGSDGGYTVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C1B0</accession>
    <name>A0A9Q1C1B0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_20316</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C1B0"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8036365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="76" mass="8505" checksum="518BAB0AEA1273DF" modified="2023-09-13" version="1">MPCDYLPRENKQVVTKQLTKPTEAPVPRRDTLTNNGKSNSEGAGSDGEDSDNKDILHNYARFLLQTEDSGQECQDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C250</accession>
    <name>A0A9Q1C250_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Homeobox protein cut-like 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_17439</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CUT homeobox family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8036800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C250"/>
    <dbReference type="OrthoDB" id="1867783at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003350">
        <property type="entry name" value="CUT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02376">
        <property type="entry name" value="CUT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 8 9" id="KW-0371">Homeobox</keyword>
    <keyword evidence="7 8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="10">
        <location>
            <begin position="160"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="8">
        <location>
            <begin position="162"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8036800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="506" mass="55704" checksum="9DED4CC523E9D6C7" modified="2023-09-13" version="1">MKGKMSSDATSYSGLEGIVVTSPDPQMAASSDAVDHLKSKGTTECSDVTGSEVNHSVSATETVEFDDDLPVFIMKFKAWMHSNQLSQRVVAPVLNLSQSHLCDLLNAKKQGNSWSNIATAGRKPYQRMKKVMEDKMILNALCHNRDVFREHSKKKRSVSRPYNPNRLVLTVHQKKILEDAYTLAKYPSATGYQKIATTMGITVRQVKTWFNNRRKLRSCPPSSQDENSYIEPMPESVSRGVIVEEFRVENGDVVAIEPYMSTNGEEVVSLPQNAALDTHDAMGENEMSGETLSSYLKALEAKKISRTEQTIGKENNVGSATSDEGNVIEMDVDSQELKTHAENKTDQLAEAVRKGGHIEVKDGVVVMTRPFPSLGGDVVMPSKEEEGGARIDHDTSPPLNPQQGFGDGLVNDGDRISSTLVVSSEKEEKTVNDDISGNVGLLAVTQQSSEKEASKMTDIQLKILQLTLEMHGNPSQEILDALMVLTCLEKEQIVEWFKNSLKGKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C2A8</accession>
    <name>A0A9Q1C2A8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">DNA (Cytosine-5)-methyltransferase 3B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_17800</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C2A8"/>
    <dbReference type="OrthoDB" id="641149at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8037072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="179" mass="20772" checksum="C248F2D76264D796" modified="2023-09-13" version="1">MRILRLLQQTTPPERPLFWFFENVVFMGHQDKMTISRFLECNPVLVDAKDVSPAHRARYFWGNLPGMDRPSAALVDSPLRLQDCLEPHCNRKAKFSKVRTITTRANSLKQGETSLPVEMDGKEDTLWCTELERLFGFPDHYTDVNNLSRGDRQKLLGQCWSVPVIHHLLAPLKDFYQCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C3E0</accession>
    <name>A0A9Q1C3E0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">U-actitoxin-Avd3s</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_18171</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C3E0"/>
    <dbReference type="OrthoDB" id="4473401at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00109">
        <property type="entry name" value="Kunitz-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.410.10:FF:000020">
        <property type="entry name" value="Collagen, type VI, alpha 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.10">
        <property type="entry name" value="Pancreatic trypsin inhibitor Kunitz domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002223">
        <property type="entry name" value="Kunitz_BPTI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036880">
        <property type="entry name" value="Kunitz_BPTI_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020901">
        <property type="entry name" value="Prtase_inh_Kunz-CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050098">
        <property type="entry name" value="TFPI/VKTCI-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10083:SF374">
        <property type="entry name" value="BPTI_KUNITZ INHIBITOR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10083">
        <property type="entry name" value="KUNITZ-TYPE PROTEASE INHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00014">
        <property type="entry name" value="Kunitz_BPTI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00759">
        <property type="entry name" value="BASICPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00131">
        <property type="entry name" value="KU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57362">
        <property type="entry name" value="BPTI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00280">
        <property type="entry name" value="BPTI_KUNITZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50279">
        <property type="entry name" value="BPTI_KUNITZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040484032" evidence="2">
        <location>
            <begin position="22"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="3">
        <location>
            <begin position="26"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8037369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="79" mass="8516" checksum="FDF164714CAED5B2" modified="2023-09-13" version="1">MKVTLVLAAFLAICFVQYAAGQEDRCSHTPGVVGPCRALIPRYTFYASSNQCELFHYGGCKGNDNNFVTKAGCEATCVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C4M7</accession>
    <name>A0A9Q1C4M7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_15763</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C4M7"/>
    <dbReference type="OrthoDB" id="10051404at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46880">
        <property type="entry name" value="RAS-ASSOCIATING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46880:SF9">
        <property type="entry name" value="ZINC FINGER PROTEIN 862"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8038365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="134" mass="15232" checksum="6E3E00A1FB42D5C0" modified="2023-09-13" version="1">MRREQARKDPGQTEASRALKMLNKQVLSRLALQFRNVHGLCKKGRPYTDYTLLCDLDEAKGMDIGKQYRTDKKAAEFATSIAEAERERMRKDFSTAKFIAAISDGTTDSSFQETEIVYVRHCQAGKNQSELFSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C5P2</accession>
    <name>A0A9Q1C5P2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">tRNA-dihydrouridine(20) synthase [NAD(P)+]-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_16465</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C5P2"/>
    <dbReference type="OrthoDB" id="10262250at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017150">
        <property type="term" value="F:tRNA dihydrouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02801">
        <property type="entry name" value="DUS_like_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035587">
        <property type="entry name" value="DUS-like_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052582">
        <property type="entry name" value="tRNA-DUS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018517">
        <property type="entry name" value="tRNA_hU_synthase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45936">
        <property type="entry name" value="TRNA-DIHYDROURIDINE(20) SYNTHASE [NAD(P)+]-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45936:SF1">
        <property type="entry name" value="TRNA-DIHYDROURIDINE(20) SYNTHASE [NAD(P)+]-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01207">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01136">
        <property type="entry name" value="UPF0034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="DUS-like FMN-binding" evidence="6">
        <location>
            <begin position="12"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8038905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="160" mass="17828" checksum="319E81048B8C254D" modified="2023-09-13" version="1">MGTHLSYADKLILAPMVRIGTLPTRLLALDYGADIVYCEEIIDHKLIKCRRVENDILNTVDFVEIDGTVVFRTCAKEKDHVVFQMGTADPQRALKVAKMVENDVAGIDVNMGCPKDYSTKGGMGAALLQHPEKIKQVCCVLMNRHSIFCTHTFCKVNSNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C6D7</accession>
    <name>A0A9Q1C6D7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Outer dense fiber protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_16664</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ODF2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C6D7"/>
    <dbReference type="OrthoDB" id="413404at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902017">
        <property type="term" value="P:regulation of cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026099">
        <property type="entry name" value="Odf2-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23162:SF10">
        <property type="entry name" value="FI13205P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23162">
        <property type="entry name" value="OUTER DENSE FIBER OF SPERM TAILS 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="126"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="240"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="443"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="531"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="626"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="52"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8039068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="851" mass="98956" checksum="E2F3A0AB89BD7441" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C7U2</accession>
    <name>A0A9Q1C7U2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="19">D(3) dopamine receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">HOLleu_13824</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">Nanhai2018</strain>
            <tissue evidence="19">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8039729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C7U2"/>
    <dbReference type="OrthoDB" id="5957871at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060170">
        <property type="term" value="C:ciliary membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00637">
        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22752">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22752:SF10">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0969">Cilium</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 15" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 15 19" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13 15" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 15 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="29"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="63"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="91"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="142"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="187"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="324"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="364"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="18">
        <location>
            <begin position="42"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="222"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="245"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="263"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAJ8039729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="409" mass="45934" checksum="C50E5DF661E466C1" modified="2023-09-13" version="1">MACPEQLKWINNHSYVEFEDYHRYLALEFLSLAFIFVSAVTLNFATIITILRVQSLREKLGNMLIINLCVMDLITSFGNMLFSLIDIFNEGYLICMNVLCWIHGGLSVIGCFGNFAAIIVIALHRFLAVVAKDNFVLTSKHIIIMITGCWVFALGVVIPLVMSTATMVYAHGNHHCSPYWGMACPQFFAFVLLMYATTLVTMISCYLAIYWKVRSSRKRLAGYGQKVKRNDETATSREQFSSTSEKGHDEGTNDPKRHHLRSDNSSQPDSGVSLTVANHQTTPNKELSEQLSVVEISETCQSGMSNIRAYKATEKLNRTPDIDIRIALTGILLVVTTIVCWTPFFVVNSCTRNKENSHEVTVSVMWLAYANSALDPIIYSVLNNKLRESILTQVRLLWSGLAGRKITST</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C7W5</accession>
    <name>A0A9Q1C7W5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_17246</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C7W5"/>
    <dbReference type="OrthoDB" id="5979489at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8039501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="95" mass="10698" checksum="63E35745095503DD" modified="2023-09-13" version="1">MALVHDHSCECAKSELDLFTIPPTQTSIERGDWKEYRPLSSINTGGPIEFQVSGSGEEYIDLDQTQLYVRAKITRIDRSALEEDDAVGHVNLFLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C8Q8</accession>
    <name>A0A9Q1C8Q8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">HMG domain-containing protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_14523</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C8Q8"/>
    <dbReference type="OrthoDB" id="4777606at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042477">
        <property type="entry name" value="HMGXB4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46584">
        <property type="entry name" value="HMG DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46584:SF1">
        <property type="entry name" value="HMG DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMG box" evidence="3">
        <location>
            <begin position="448"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="1">
        <location>
            <begin position="448"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="307"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="408"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="517"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="47"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="103"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="176"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="201"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="222"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="233"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="319"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="431"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="517"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8040283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="623" mass="70119" checksum="50E2149B63919F94" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C986</accession>
    <name>A0A9Q1C986_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Tyrosine-protein kinase receptor Tie-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_14760</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C986"/>
    <dbReference type="OrthoDB" id="10252017at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.300.10">
        <property type="entry name" value="Tie2 ligand-binding domain superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013111">
        <property type="entry name" value="EGF_extracell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26391">
        <property type="entry name" value="INACTIVE TYROSINE-PROTEIN KINASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26391:SF18">
        <property type="entry name" value="PROTEIN KINASE RECEPTOR TIE-1, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07974">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="210"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="227"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8040464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="457" mass="49851" checksum="6581C65B0E71C97A" modified="2023-09-13" version="1">MDPYPEYYCYLNSPDATVTAMRVWDTRTDRANSLTNNPDPPPPQLIMPHSSYPAGSAVYKVSLSFNMNIQSYAFGTFSCDASKDDRKTTRVSNILVNSNVHSLPADGLVTQTVNEGDTGVEIRMMSHGHDVVGWYRNEERIGTSSRFTASGGTLSINNTVEKSLHEGVYEAITDFQGQPRVQPRHGLKRLIVRSCAANHWGPCGCTGICDNCYNGGVCDDETGRCICPPGFMGTNCLTVKAHMEPVVFRIVTVMDSVINLLVCVMESVKLAGVDRAVRECRCSADACNKVTGECSGQCKSQWVDAYQCLAGVESVTSSKINPNTTSDINCVTQIFRNSSEVLTLNLSRETDQIVNHKIQFVNRTMMGDTEVWRFTASDVKDGDELFCLLLLGDDISASLNGDLGILTGSPGETLEFMADNLEADTEYSFSVAVVREGVNGEGPRGPSVNLRTKCDGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CA88</accession>
    <name>A0A9Q1CA88_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_15417</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CA88"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040430709" description="Ig-like domain-containing protein" evidence="2">
        <location>
            <begin position="24"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="398"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8040956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="478" mass="53896" checksum="28F3CD51C5DCCBF1" modified="2023-09-13" version="1">MDLNSRLGSFVFCFVILQVPSLSQTHLIVNIEVSGNNVVKTGIREYTVSTYYGSTVVIKCSSRPTLSSNLVLWKNNEPIAENVGVSAHEITFEEYYSMQTVNRMVQKYHCVSSISKRILNDSYAHFTVWNEVDIRMEPDSVLCASSVGRSGYLGEKATLQCMSLDRNYRCFFEGQRSWNITRQKVLVNTLSETTCNCRYRRRYYYDYSYRSCLLHSISVVEFVILNIFNTNNVTKTDTSLVELVCTSRPPRHMFWAVFAGDSLLFDFNTLNSTSFKVNTTVNITQSSGETTLSILESVPGGNGIHTVICSTVDTEARAVALYRIQHQSVRNLPGCDLSSSKTTEMQVTTDTTYDNPTSTDEGSRFVLTTTEVLKDSKEYETNQIEHGHEIKMDSANNYFLMGGVGLPLLLTNLILIVAVFYLHKKTRYKAGINAQVSTQSTGSPDIELHDNPTYLAFTETEGTLCNENEERECTYANM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CBK8</accession>
    <name>A0A9Q1CBK8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_12893</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CBK8"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8041950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="56" mass="6535" checksum="51B54E46AE144676" modified="2023-09-13" version="1">MNGRNETFEVQSTRANRRANMVKYVLCGLLSIFQMVNTSQKKMSKMQTIVPVREIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CCD5</accession>
    <name>A0A9Q1CCD5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Sulfotransferase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_09591</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8042746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CCD5"/>
    <dbReference type="OrthoDB" id="2405944at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48312">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48312:SF1">
        <property type="entry name" value="SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040181532" description="Sulfotransferase family protein" evidence="1">
        <location>
            <begin position="17"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8042746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="303" mass="35840" checksum="4E95C0BF186EBDDC" modified="2023-09-13" version="1">MAESTIFIWCWPATAATVLGKCLSNVDEVEYWHDPYWNCYNIEQLGDLKMETLSPELQKYFTKIREAAVPTDGKKFFEGSESLPSETFKYPIIKEKLEEGLTGRKFRIIKDVSRSMINHEEFLAEVPARHVFLIRHPYRVFYSMTRTVREMCQRIGFGEVDFDMFRGLPSFHEYVDKDHCHTLWKHLQETQNVTPLIIETEDLVNHPDKILPELFRYSGIPFQEKYLKWSESRDVLKKWKTSERSMVANWGLKTFERAFTSSSFVPHSNPLPKKEDLPSVYSEHIHILVKSYEEMHNQRFQPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CDT6</accession>
    <name>A0A9Q1CDT6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Sphingosine 1-phosphate receptor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_10077</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CDT6"/>
    <dbReference type="OrthoDB" id="5981855at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00637">
        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24243">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24243:SF208">
        <property type="entry name" value="PYROKININ-1 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="29"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="66"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="110"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="151"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="204"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="249"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="288"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="10">
        <location>
            <begin position="45"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8043116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="343" mass="39428" checksum="B5BE8BAA4EB73E50" modified="2023-09-13" version="1">MTSNNTTTCSNELVVENISFLFYSPFEKFIQINIVPCLAVFGVLCNLSFLLVVYRVKQFHNGINVLLVNLAIADIFFLITDVTNTLSTVISSPYQRDFSWSPVDWGCTLVNGAVHLFEASSLLFIVIISIDRYFAVCRPFQHRLSKHLRTFLFCAIPWGTAVVLAVFYSLTLEEWDICWEWKYDNATLPTTYSTCEPSQDTGPFFYVLNVAPYVISVCVNGAFYYWILKTLKRSYAVNSQRSVEARMSAVKMLVFNWLVYLVCVSPNFVLLFVNVIYDIDWLKKVEPICRALLVINSSVNPLIYNAFNSRYRKAFVRVFSFRRKTRPSRVSFSGHSSVRTTKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CDT9</accession>
    <name>A0A9Q1CDT9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_09696</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8042834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CDT9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8042834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="52" mass="5931" checksum="9B3A73471EFE2D26" modified="2023-09-13" version="1">MRCGERKNPIIFGGGQRSSGVTGGQNLKTLFTRYLKLESLDRCHAWYVDALW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CE17</accession>
    <name>A0A9Q1CE17_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">2-(3-amino-3-carboxypropyl)histidine synthase subunit 1</fullName>
            <ecNumber evidence="4">2.5.1.108</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Diphthamide biosynthesis protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Diphtheria toxin resistance protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">S-adenosyl-L-methionine:L-histidine 3-amino-3-carboxypropyltransferase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HOLleu_10764</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Nanhai2018</strain>
            <tissue evidence="16">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-histidyl-[translation elongation factor 2] + S-adenosyl-L-methionine = 2-[(3S)-amino-3-carboxypropyl]-L-histidyl-[translation elongation factor 2] + S-methyl-5'-thioadenosine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36783"/>
            <dbReference type="Rhea" id="RHEA-COMP:9748"/>
            <dbReference type="Rhea" id="RHEA-COMP:9749"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:29979"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73995"/>
            <dbReference type="EC" id="2.5.1.108"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DPH1/DPH2 family. DPH1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.108" evidence="4"/>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CE17"/>
    <dbReference type="OrthoDB" id="1649088at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090560">
        <property type="term" value="F:2-(3-amino-3-carboxypropyl)histidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11850:FF:000001">
        <property type="entry name" value="2-(3-amino-3-carboxypropyl)histidine synthase subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11860:FF:000002">
        <property type="entry name" value="2-(3-amino-3-carboxypropyl)histidine synthase subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11840">
        <property type="entry name" value="Diphthamide synthesis DPH1/DPH2 domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11850">
        <property type="entry name" value="Diphthamide synthesis DPH1/DPH2 domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11860">
        <property type="entry name" value="Diphthamide synthesis DPH1/DPH2 domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016435">
        <property type="entry name" value="DPH1/DPH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042263">
        <property type="entry name" value="DPH1/DPH2_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042264">
        <property type="entry name" value="DPH1/DPH2_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042265">
        <property type="entry name" value="DPH1/DPH2_3"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00322">
        <property type="entry name" value="diphth2_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10762:SF1">
        <property type="entry name" value="2-(3-AMINO-3-CARBOXYPROPYL)HISTIDINE SYNTHASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10762">
        <property type="entry name" value="DIPHTHAMIDE BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01866">
        <property type="entry name" value="Diphthamide_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00032">
        <property type="entry name" value="Radical_SAM_3-amino-3-carboxyp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="286"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00032789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ8043592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="353" mass="38944" checksum="6D6E1BF72E95E341" modified="2023-09-13" version="1">MGDVTYGACCVDDFTAKALGADLMIHYGHSCLIPINVTTGINMLYVFVDIKIDVAHFIDTVRHNFSPGTSLAMVSTIQFVAAIQAAGRDLTKDYSITIPQAKPLSPGEILGCTAPKVSGVDAIIYLGDGRFHMEAAMIANPDIQMYRYDPYTKIFSKEYYEIDRMHDLRKSAIQVASQAKKFGLVLGTLGRQGSPKVLEQLTTKFKAAGIDTVTVLLSELFPDKIKLFEDVDAWVQIACPRLSIDWGYAFPKPVLSPYEASVVLNLLEWQQDYPMDFYANDSLGPWTPNNEANRPPRPSRGNRRGAPKVSVKEEKDPVKQTAGEESSDCLKCTNCECGNSKVEKAIPDKSGDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CET0</accession>
    <name>A0A9Q1CET0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein rolling stone</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_10189</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CET0"/>
    <dbReference type="OrthoDB" id="419711at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12242:SF45">
        <property type="entry name" value="MARVEL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12242">
        <property type="entry name" value="OS02G0130600 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="173"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="205"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="244"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8043204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="317" mass="36616" checksum="05A8F8ED736CD390" modified="2023-09-13" version="1">MGYVRNVLCLVNVYPEVFGRPQWQFSQVLYIYYRYLFAFYVIGWDVFITIRGSYQYGGWYQIDLKVWTFHLLALYAFISVVTALVHWVRVAKWRYEDFLRKDSSPAIDSVLNTSQNSLGITADQSIYPSNPWYFKLDWLLFNLNISLHPIVTILFWIHARNDNVFSSDDKSDILAVAHLTIVNTALIVLDLFIRRIPVYILQAAYPMTVWIIYIIVNVIVHFANAPGSQEFIYSDLFNWSADNVAPSISFAVAFLFGILLVHLLAYFLFKFRTALFLRCCKDVGYYDSSGVSLEMSSGLCCSVCGRGQDSHNFVFNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CFP8</accession>
    <name>A0A9Q1CFP8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Dehydrogenase/reductase SDR family member 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_11464</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8044095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CFP8"/>
    <dbReference type="OrthoDB" id="1933717at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000047">
        <property type="entry name" value="NADP-dependent L-serine/L-allo-threonine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43115">
        <property type="entry name" value="DEHYDROGENASE/REDUCTASE SDR FAMILY MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43115:SF4">
        <property type="entry name" value="DEHYDROGENASE_REDUCTASE SDR FAMILY MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8044095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="257" mass="28104" checksum="5D5B04FC55E338E9" modified="2023-09-13" version="1">MSEKARWVGRVALVTGASAGIGEATAKLLVQEGMKVVICARSIDKLQAIAEDLNKGKTDRCCLPVKCDVSKEDQVLEMFKQIRSELGRLDICINNAGINVKNCSLLEGSTETWQKIIDVNVIGLSICTREAVKLMREKGIDDGHIIHLSSFSGHRVPPGNAVTNFYAGTKFAVRALTEGLRQELRAIKSNIRVSSLSPGLVKTDFNADVIEGDYEAFISSNPTMSSEDVAQLVLVCIQQPAHVQIHDILVRPTQQLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CHQ3</accession>
    <name>A0A9Q1CHQ3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_07853</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CHQ3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8044948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="153" mass="16593" checksum="9E44F91A2C8F9FF2" modified="2023-09-13" version="1">MEIEEGNSNANSDEERHEAKVTHESRSPQLKVQDSVEPEGKGNSTDVLINQRPVATVTIESNSPQLEVECSMETEATCEGSLANVINEKGLKAILTSESTSLQLEVKDSMETEEGNSTGALIDQRPMATVTIESNSPQLEVECSLETEGIIMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CHR7</accession>
    <name>A0A9Q1CHR7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">DnaJ-like subfamily C member 17</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_08565</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CHR7"/>
    <dbReference type="OrthoDB" id="259708at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000390">
        <property type="term" value="P:spliceosomal complex disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12429">
        <property type="entry name" value="RRM_DNAJC17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034254">
        <property type="entry name" value="DNAJC17_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052094">
        <property type="entry name" value="Pre-mRNA-splicing_ERAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44313">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44313:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="J" evidence="9">
        <location>
            <begin position="11"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="10">
        <location>
            <begin position="175"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="262"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="310"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="72"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="262"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="313"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="338"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8045536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="400" mass="46139" checksum="47B3DCCAC860BFB3" modified="2023-09-13" version="1">MAPSKPTNDQDFYEVLDLLPSATEKEIKKAYRKKALKCHPDKNPDNPQAAQEWERLSKALEVLTDTSAREAYDKVLKAKKAAELRNRELDSQRKKLKQDLEAREAASQRIKESAADAAKRLEREIKRLREEGSRILEEEQERLRKEVREAPVEESPKLRVKWKCQKGEEGNGGYSEEYLKECFEEYGEVTHIIVSRKKKGSAIIEFASVNGAERAVANVIGHTDNPLQVSWLSGRPTSNFYAMKEEEKGENFEQMVLEKMKRTQRMKDERMRNDEAERHQRGKDAKSTNLSESAENVEINEADILQKLKQARAESSTKQPSWIQSSNQFSFQTFKPFESSKGDAEDGFSKTGQQNFEEGNILKKSASQARDFETIAMRKLRQAEERRKLIEQMQREDADD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CI52</accession>
    <name>A0A9Q1CI52_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Iodotyrosine deiodinase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_08942</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nitroreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CI52"/>
    <dbReference type="OrthoDB" id="41362at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140616">
        <property type="term" value="F:iodotyrosine deiodinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006570">
        <property type="term" value="P:tyrosine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02144">
        <property type="entry name" value="iodotyrosine_dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.109.10:FF:000004">
        <property type="entry name" value="Iodotyrosine deiodinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.109.10">
        <property type="entry name" value="NADH Oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029479">
        <property type="entry name" value="Nitroreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000415">
        <property type="entry name" value="Nitroreductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050627">
        <property type="entry name" value="Nitroreductase/BluB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23026:SF90">
        <property type="entry name" value="IODOTYROSINE DEIODINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23026">
        <property type="entry name" value="NADPH NITROREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00881">
        <property type="entry name" value="Nitroreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55469">
        <property type="entry name" value="FMN-dependent nitroreductase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0288">FMN</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitroreductase" evidence="6">
        <location>
            <begin position="23"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8045842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="219" mass="25527" checksum="38B4D5EEAF3A0A6A" modified="2023-09-13" version="1">MKYNFTEEEMVERSEQFYKEMNDRRSVRFYSDRPVPLRVIENIIKTAGTSPSGAHTQPWVFSVVRDQEMKRKVREIIEDEEKINYERRMGKKWLDDLKPWGNTYKKPYLSKCSTLIVLFKQVHSFTEDGEKKTHYYHEISASISVGLMLAAIQQNAGLVTCTTTPMNAGPRLRKLLGRKSYEKVMLLFPVGYAADNATVPDLQRKPLEEIMDLHDQAEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CK05</accession>
    <name>A0A9Q1CK05_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_08980</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CK05"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8045874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="119" mass="14120" checksum="FB8C6211C9C18EC1" modified="2023-09-13" version="1">MTILRNQRKDEKFRSDIKELKENLGLIKARMDMITTYRLAKRKQTNFQKKICQARRNFWKELPHIEHFFVCAACVMFASLLCLYLVRALVLLLLPQIFLECHPNISQVLKYKINSSLCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CKB0</accession>
    <name>A0A9Q1CKB0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_05194</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CKB0"/>
    <dbReference type="OrthoDB" id="6362117at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032502">
        <property type="term" value="P:developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000870">
        <property type="entry name" value="zinc finger protein 197 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001136">
        <property type="entry name" value="Zinc finger protein 408"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000202">
        <property type="entry name" value="Zinc finger protein 574"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000110">
        <property type="entry name" value="Zinc finger protein-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24376:SF235">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24376">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="15"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="259"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="287"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="314"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="342"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="370"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="398"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="426"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="454"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="482"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="510"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="538"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="566"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="594"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="622"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="650"/>
            <end position="677"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8046506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="682" mass="79801" checksum="39B52A35B9E79BD2" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CKF4</accession>
    <name>A0A9Q1CKF4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_04980</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CKF4"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8046340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="211" mass="24876" checksum="E9233512134758C7" modified="2023-09-13" version="1">MLDDNNLNTQRRNGVRQFITSVRYQWRASGLMFRQECFRVSVNLCFPACTSMVGFFLCAFFSYRMEEAQEQTVIYFCLCHWVTCLAGKAIHLPTLLSQEAEVVPLRNRLTLFLLCMYSILTVLEVWIILTVKSIDTECITAELRRSSMRYYARHCHIYSELSFSWVKWVISLGYKRTLQVEDFGTLLDHLGAQRLQEEFDKIFMEEKVSLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CP05</accession>
    <name>A0A9Q1CP05_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_00724</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CP05"/>
    <dbReference type="OrthoDB" id="10068393at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46601:SF1">
        <property type="entry name" value="ADF-H DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46601">
        <property type="entry name" value="ULP_PROTEASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8048415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="198" mass="22447" checksum="3A20401D6D0D106F" modified="2023-09-13" version="1">MDFAENYTCVPLEEVQSGYWNQEGVTLHPMVMYYRDENQTLKHHSFVAVSDDKNHNATTIFSIVKDLVPRLVKIVPSLKRVHYWTDSPTAQYRNKTIFSLVSDHHELFNGIFASWNFFEAGHGKGPCDGIGGTVKRLADEAVKREAAVIQDANDFYAWASQEREASDIEYVFVSKDDCAANAQEITKRWPSVKAIAAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CPK9</accession>
    <name>A0A9Q1CPK9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_01622</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CPK9"/>
    <dbReference type="OrthoDB" id="5954868at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012444">
        <property type="entry name" value="DUF1647"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31389">
        <property type="entry name" value="LD39211P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31389:SF4">
        <property type="entry name" value="LD39211P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07801">
        <property type="entry name" value="DUF1647"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8049061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="385" mass="43402" checksum="83666BC0E4ED9BCD" modified="2023-09-13" version="1">MLPLSVQAESVSILISRNGKGKQWLSITAFVLSFFSIVSLYADVSSLTGAPLRTLLKPKAVFKRTSLVKSRLLIFNKSNTIHPQVEIKTENERKFAKNYDEYRATSTDKEIFIIDLDKTEPPLNFLYEKLTLVTSFSENHFQEGLGFIGSAQQQMPNKTIIVYDLGLKEHSSNQIRSFCNVQLRRFPFESYPAHLKTLATYAFKPTIINLVLNEFGVIYYGDASIRFKKPLFELLPDCVNHHGFLGQIRCFDPKVLKSKYGCRHDYGLTKEEIYGKIGVNKTEYYNANFSSPAISGGLMLVINSSSVQSKIMRPWLSCALDVTCIAPHGANLGNHRFDQSALSLIAFKNMRGEWTLENDPTPHHNAVVSVLRGSNGAKWRPQSCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CPL5</accession>
    <name>A0A9Q1CPL5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Reverse transcriptase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_01174</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09274">
        <property type="entry name" value="RNase_HI_RT_Ty3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000115">
        <property type="entry name" value="Polyprotein of retroviral origin, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.370:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 17.6-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000032">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 297-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon gypsy-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054465">
        <property type="entry name" value="Integrase_p58-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041373">
        <property type="entry name" value="RT_RNaseH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22938">
        <property type="entry name" value="Integrase_p58_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17917">
        <property type="entry name" value="RT_RNaseH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="9">
        <location>
            <begin position="883"/>
            <end position="1041"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase" evidence="8">
        <location>
            <begin position="1297"/>
            <end position="1474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="94"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="352"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="616"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="716"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="352"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="367"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="391"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="616"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="640"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="660"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="685"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="732"/>
            <end position="756"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8048741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1689" mass="193493" checksum="E4D55B87198CFB64" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CPS4</accession>
    <name>A0A9Q1CPS4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_01307</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CPS4"/>
    <dbReference type="OrthoDB" id="6496131at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33198">
        <property type="entry name" value="ANK_REP_REGION DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33198:SF19">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="187"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="187"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8048835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="262" mass="29436" checksum="E455F8C7D1CE0D3E" modified="2023-09-13" version="1">MAERGYFIGSVGRFKSAKEDWVTSQKRLEIWFKANKISDNNKVNVFLAKIGPETFEVENHIPPADPTTRYQIASRLKFRSRKQQPNETIADYILELKKLSRHSSYGEQLDTNLRDTFVGGLANPKVQAKLLQRGDRLTWKTAREDALTIEKALKDSKQLSDLSGGSDCPHKTAKGFGCNKVGHLKRACHPNRVKNSRKTSAPTRGGKPRRGQGRSQGQRNAHMVSPEDSDSTDTSGEADFIGTLFQTTQLTKKSHHTPWLCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CR73</accession>
    <name>A0A9Q1CR73_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Low-density lipoprotein receptor-related protein 8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HOLleu_02807</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Nanhai2018</strain>
            <tissue evidence="17">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LDLR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CR73"/>
    <dbReference type="OrthoDB" id="5958943at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000009">
        <property type="entry name" value="Low-density lipoprotein receptor isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000002">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000241">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051221">
        <property type="entry name" value="LDLR-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000033">
        <property type="entry name" value="LDLR_classB_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22722">
        <property type="entry name" value="LOW-DENSITY LIPOPROTEIN RECEPTOR-RELATED PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00058">
        <property type="entry name" value="Ldl_recept_b"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00261">
        <property type="entry name" value="LDLRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00135">
        <property type="entry name" value="LY"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101898">
        <property type="entry name" value="NHL repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51120">
        <property type="entry name" value="LDLRB"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 13" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="4" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="17" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 17" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="229"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="15">
        <location>
            <begin position="357"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="15">
        <location>
            <begin position="442"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="15">
        <location>
            <begin position="489"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="43"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="83"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="108"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="115"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="174"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="181"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ8049878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="630" mass="70337" checksum="B1735E5EEFE16A2B" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CRI9</accession>
    <name>A0A9Q1CRI9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_03108</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CRI9"/>
    <dbReference type="OrthoDB" id="10034966at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
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        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8050058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="502" mass="56852" checksum="68B448A6FC0AB3CD" modified="2023-09-13" version="1">MIDEESLLLLTKEEIKELITKVGPRMKLLKNLEELKNGRVTDRYENTSTALKCACNDEPAAVHIQGDDLPRCEHEQCEEGGGEIDIADRDADIDTYEDWDHLDEQELTERALMLVCKLKSSSSVSQSVVSNIVQDVTQLCGDLVGNLKESTKAFLVNHDIDTQSEAALLLFEKFEMFQNPFHGVETDYKQQQYLLASKMFIPPVAKEITGYAPRTDSATGNVLQLPTPRVFHYVSIKKVVKLVEQPGFWECVEEHRKSTDGVMRDFHDGDYCAAHHLFMTRNCLKLILYNDDVEMTNPLSPKAGSHKLGVLYYTFKDIHPCHLSTLSHFYLAALYKSSDVSEFGFDVILQPLVDELKELESVGMEIDCPSFRGIIKVGLAQVIGDNLGIHSILGFSQGVTANYFCRKCKGYRDNMRMETKLSANLNRTVEENYTQDLLADNLPLTGVRTSCLLDELESFHVTKNYAPDIMHDMLEGVCPLELKLVLCRLIENRFFDLNTLEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CSB3</accession>
    <name>A0A9Q1CSB3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_03690</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CSB3"/>
    <dbReference type="OrthoDB" id="10068564at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF11">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8050471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="95" mass="10782" checksum="9C57410D3F347692" modified="2023-09-13" version="1">MHVDESVAPVAVRYGKVPYALRKVVEPKLRELEENNIIEDATDSTPWVSPMVIIDKPKDPTDFRPCIDMRTANEAILRERHDTPTVEELIAEVNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1GW23</accession>
    <name>A0A9Q1GW23_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_29827</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8027777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1GW23"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8027777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="101" mass="12392" checksum="AD8E8412B95C2FA0" modified="2023-09-13" version="1">MRKDEIKRYVLEYLVDESILPITVLETAITVPTDNTFELKRLEIEMNKEIRLKEKEEREMQMQMQKEKEEREMQMQREKEAREHEFRLKQLELGVIKGLRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1GZF1</accession>
    <name>A0A9Q1GZF1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Calcium-activated chloride channel regulator 4A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_30334</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1GZF1"/>
    <dbReference type="OrthoDB" id="687730at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="CDD" id="cd00198">
        <property type="entry name" value="vWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013642">
        <property type="entry name" value="CLCA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051266">
        <property type="entry name" value="CLCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10579">
        <property type="entry name" value="CALCIUM-ACTIVATED CHLORIDE CHANNEL REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10579:SF177">
        <property type="entry name" value="CALCIUM-ACTIVATED CHLORIDE CHANNEL REGULATOR 4-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08434">
        <property type="entry name" value="CLCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="973"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="2">
        <location>
            <begin position="343"/>
            <end position="517"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8028168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1025" mass="113771" checksum="A57624BAA8FD76EE" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H0I5</accession>
    <name>A0A9Q1H0I5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Complement C3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_29792</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8027751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6359008at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004866">
        <property type="term" value="F:endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd02896">
        <property type="entry name" value="complement_C3_C4_C5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.130.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.690">
        <property type="entry name" value="Alpha-macroglobulin, receptor-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009048">
        <property type="entry name" value="A-macroglobulin_rcpt-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036595">
        <property type="entry name" value="A-macroglobulin_rcpt-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050473">
        <property type="entry name" value="A2M/Complement_sys"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011625">
        <property type="entry name" value="A2M_N_BRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011626">
        <property type="entry name" value="Alpha-macroglobulin_TED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003410">
        <property type="entry name" value="HYR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001599">
        <property type="entry name" value="Macroglobln_a2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001134">
        <property type="entry name" value="Netrin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018933">
        <property type="entry name" value="Netrin_module_non-TIMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008993">
        <property type="entry name" value="TIMP-like_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11412">
        <property type="entry name" value="MACROGLOBULIN / COMPLEMENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11412:SF166">
        <property type="entry name" value="NTR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00207">
        <property type="entry name" value="A2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07703">
        <property type="entry name" value="A2M_BRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07677">
        <property type="entry name" value="A2M_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02494">
        <property type="entry name" value="HYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01759">
        <property type="entry name" value="NTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07678">
        <property type="entry name" value="TED_complement"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01360">
        <property type="entry name" value="A2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01359">
        <property type="entry name" value="A2M_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01361">
        <property type="entry name" value="A2M_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00643">
        <property type="entry name" value="C345C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49410">
        <property type="entry name" value="Alpha-macroglobulin receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50242">
        <property type="entry name" value="TIMP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50825">
        <property type="entry name" value="HYR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50189">
        <property type="entry name" value="NTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0768">Sushi</keyword>
    <feature type="domain" description="HYR" evidence="7">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="8">
        <location>
            <begin position="75"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="8">
        <location>
            <begin position="138"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="HYR" evidence="7">
        <location>
            <begin position="201"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="NTR" evidence="6">
        <location>
            <begin position="1357"/>
            <end position="1497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8027751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1498" mass="165883" checksum="C4B39C630241E0C4" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H0P7</accession>
    <name>A0A9Q1H0P7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Kelch-like protein 9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_28710</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H0P7"/>
    <dbReference type="OrthoDB" id="45365at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="CDD" id="cd14733">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017096">
        <property type="entry name" value="BTB-kelch_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632:SF10">
        <property type="entry name" value="BTB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632">
        <property type="entry name" value="LD33804P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037037">
        <property type="entry name" value="Kelch-like_protein_gigaxonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BTB" evidence="4">
        <location>
            <begin position="109"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="70"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8029339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="663" mass="74491" checksum="688AD8C4DF3C1ABD" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H1W6</accession>
    <name>A0A9Q1H1W6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_28335</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H1W6"/>
    <dbReference type="OrthoDB" id="6098717at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="59"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8029041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="214" mass="24157" checksum="22E9EE23CABBBCCA" modified="2023-09-13" version="1">MTNALFRPNENVNSNAGTEDWSEENFQQDNDKTLSMDNQSVSVKSDGGSVTNKDTDNPSTSTSTKASSNNVGFASRFQQLRPWGPIWGMSSPLAYEVGLDEEVLALLAYANFEVNMFRRECIKPDLNPKYHPLCKADVKSFWKDNTDIHVQIMIDNVTAVTYINNMGGIRLSWDLCKHEEYQKKLEKLSLHHGGSQLDLSMAPPLLQIQIWIDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H208</accession>
    <name>A0A9Q1H208_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_28528</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H208"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8029191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="122" mass="13782" checksum="60AB1CA0B8544BDE" modified="2023-09-13" version="1">MMYDALRRQSISMSGVDTREGRNDKNVNSFGPTGFQVFPCSEDHQQVRHIQYPHHHGPGGDINRTIHQSPPEREFVLQPADSAASTPQHRIRANLSAVQDRFNSNFIGTPNDISRPLGHRKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H2E0</accession>
    <name>A0A9Q1H2E0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_27522</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H2E0"/>
    <dbReference type="OrthoDB" id="6507183at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000032">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 297-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon gypsy-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF1">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="297"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase catalytic" evidence="4">
        <location>
            <begin position="691"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="31"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="254"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="978"/>
            <end position="1054"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="254"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="269"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="978"/>
            <end position="992"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1018"/>
            <end position="1027"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8030959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1054" mass="120495" checksum="C6D0F9881F92332E" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H559</accession>
    <name>A0A9Q1H559_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_19690</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8035877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H559"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8035877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="92" mass="10751" checksum="9927DD92D4A93FB0" modified="2023-09-13" version="1">MEICCNYLVLIITFIITLPVEWWIADTFDYYDKSSSCEPIHVEGYHTCKVLGLLMPEKWIVMIIEVVVTSVLGCCLKRVFSLCCNCDRYPRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H5H3</accession>
    <name>A0A9Q1H5H3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_23225</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H5H3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8033091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="82" mass="8773" checksum="EA920440676B8F0B" modified="2023-09-13" version="1">MRLPDPGELDSTIQIEGKRKHDVVPVASPRGFKGGQVLQLLHNALSVYGCNTTAVLPSLCSSVGILLEAMSIENAKFAVRVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H5K9</accession>
    <name>A0A9Q1H5K9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_23472</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H5K9"/>
    <dbReference type="OrthoDB" id="5986708at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="179"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="190"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8033285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="217" mass="24277" checksum="524E9C44AED7CDEB" modified="2023-09-13" version="1">MKKAKLDFVSLQVKVKRFLLSYRTTPHSFTRETPAKLFLNRELRTRLSVIKPNFGHSISQKQSSKQSSTRLLSVGEKVRVLDFKKQTGKWSQGVVLKVLGPVTYLVCVDNQFWKRHIDQIRSMHQTNPTVPCPMPDGKVLGEVEGLDTASDLDVEVPVGFPRNKREHNSDMLMPNETHSKVEDQNVQTEAASSPIVAPAPVTPVKPSVSTPDPGHLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H5U6</accession>
    <name>A0A9Q1H5U6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_24332</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H5U6"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8033943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="83" mass="9593" checksum="7951EB4696916055" modified="2023-09-13" version="1">MRCPKFEFMLAKQRQTRQNSDRLVKTTFACLENLKSESIVILAARSLIDLSLAYRIDLFVRKAHHIARYISVPPLGEFNPIDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H6J3</accession>
    <name>A0A9Q1H6J3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_21041</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H6J3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8034275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="126" mass="14061" checksum="1A5C7C9375241A43" modified="2023-09-13" version="1">MLLCFNTISKKAFASKFVDVGPSESVLIASLDNAANLQNKEKSMRMALHDEVFVSGVSVMEKSIKKFNGYSGKEGFPIFAKIRLILAFEVDVTFKCELWHGVEFARHYHASVVKPTDAQELIFISA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H7H2</accession>
    <name>A0A9Q1H7H2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Histone-lysine N-methyltransferase EZH2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_22397</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H7H2"/>
    <dbReference type="OrthoDB" id="6141102at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035098">
        <property type="term" value="C:ESC/E(Z) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046976">
        <property type="term" value="F:histone H3K27 methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031507">
        <property type="term" value="P:heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045318">
        <property type="entry name" value="EZH1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45747">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE E(Z)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45747:SF4">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE E(Z)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="SET" evidence="4">
        <location>
            <begin position="14"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8035236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="103" mass="11959" checksum="2B4EE1CB8CD2B699" modified="2023-09-13" version="1">MQGSNVDMDHLPQDQSIISQDEADRRGKVYDKYMCSFLFNLNNDFVVDATRKGNKICFANYSVNPNCQAKVMMVNGDLCQERNIQEGEELFFDYRLDELHHVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H8E6</accession>
    <name>A0A9Q1H8E6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">TIR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_20328</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H8E6"/>
    <dbReference type="OrthoDB" id="9978456at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46270">
        <property type="entry name" value="ARMADILLO-TYPE FOLD-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46270:SF2">
        <property type="entry name" value="TIR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13676">
        <property type="entry name" value="TIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TIR" evidence="1">
        <location>
            <begin position="158"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8036375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="387" mass="44010" checksum="D35E351B43FB44A1" modified="2023-09-13" version="1">MLSDEEWSVILQYKDGKDEYLKAISVMILAFLSDEKTEGIKGEEDTTIYMLKLLKEAKQDSSRRKQGFSCTELIEGLNRLALNDSNKHVILENDGIELLMEFIMSEEPSEPEAAAQCLWTLAFDPSNSIQARLEKHVSTLEDVFKLCDNSKVKGGGHIMVSYAWDGGYGQETARRIAANLKSRGLKVWIDVDEMKGGVFDAMAEAVNNSYVFILCVSDKYRKSNNCRLEAKYAYQHGKLMIPVQVSASISSKSDWLRFLCSDSLYVDFTEGNNFRQRCEDLVEQISRLTRGEDTIDNAPLNSSVLSRQPPSASGWSPDDVNKWLRDNDLGRFSELFRQRDGSTILALKKLRREAPNFFYQCLWELDSKEKRDKLIAILKLTQALDSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H8R8</accession>
    <name>A0A9Q1H8R8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Macrophage mannose receptor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_20811</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H8R8"/>
    <dbReference type="OrthoDB" id="441660at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050111">
        <property type="entry name" value="C-type_lectin/snaclec_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22803">
        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0675">Receptor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040245635" evidence="3">
        <location>
            <begin position="27"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="668"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="4">
        <location>
            <begin position="37"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="4">
        <location>
            <begin position="191"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="4">
        <location>
            <begin position="338"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="4">
        <location>
            <begin position="476"/>
            <end position="596"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8036748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="734" mass="80988" checksum="1C0F681AA697E493" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HAP7</accession>
    <name>A0A9Q1HAP7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_16562</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HAP7"/>
    <dbReference type="OrthoDB" id="425681at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF20">
        <property type="entry name" value="REVERSE TRANSCRIPTASE-LIKE PROTEIN WITH RNA-DIRECTED DNA POLYMERASE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8038985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="255" mass="28726" checksum="268333521A3286E2" modified="2023-09-13" version="1">MEGRVNMCGELSEPFLINNGVKQDCLLAPTLFRLFFAAVLQDATSDLRTGVFLQTRTDGGLLNLSRLIAKTKPAPGNAYEEPTIAIDNTKLNAVTHFCYLGSIMSTSASLDLEIESRIRKASFAFGKLRDRVWSQNIRLATKCKEYRALVIQLYCMDVKHGAHIRDICVSLTNYSSDLAQKRAGIDSSSWEELAQNPTKWRASIRKGAKVAEDSLRRRAKDNRRKRQNRASAIFSPRCIHLLCLWKTVSLQNRTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HB53</accession>
    <name>A0A9Q1HB53_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Plancitoxin-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_16649</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNase II family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HB53"/>
    <dbReference type="OrthoDB" id="10261598at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004531">
        <property type="term" value="F:deoxyribonuclease II activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006309">
        <property type="term" value="P:apoptotic DNA fragmentation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09121">
        <property type="entry name" value="PLDc_DNaseII_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004947">
        <property type="entry name" value="DNase_II"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10858">
        <property type="entry name" value="DEOXYRIBONUCLEASE II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10858:SF23">
        <property type="entry name" value="DEOXYRIBONUCLEASE II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03265">
        <property type="entry name" value="DNase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8039058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="250" mass="28567" checksum="484E5BBBA8381F3F" modified="2023-09-13" version="1">MIHNLPRFPQEDEYEWASNAGPNGQSILCVSFKSSEMEKIAKQMRFTYPYVYVHNLPPDKLRIAPTFHEVVSREPVTITPSTNAVSLESLGGQRFTHYAMKSSFGQDLYLDLVAQEQRSNLLVETWQRKGPLPSNCSHGYCVRNIRTIRFPDTGVEFPYTKDHSKWAVSDDNKKIICVGDNNREDPHLRRAGGILCSHLPNVWKQYKDSVVQVEPLPVDGLYSKALKIFLGVATIFVGARWYKNRYEGLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HD28</accession>
    <name>A0A9Q1HD28_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Neuralized-like protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_12462</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HD28"/>
    <dbReference type="OrthoDB" id="10059069at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03587">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12887">
        <property type="entry name" value="SPRY_NHR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.750.20:FF:000001">
        <property type="entry name" value="Ankyrin repeat and SOCS box containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.750.20">
        <property type="entry name" value="SOCS box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037962">
        <property type="entry name" value="Neuralized"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006573">
        <property type="entry name" value="NHR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001496">
        <property type="entry name" value="SOCS_box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036036">
        <property type="entry name" value="SOCS_box-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12429">
        <property type="entry name" value="NEURALIZED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12429:SF8">
        <property type="entry name" value="NEURALIZED-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07177">
        <property type="entry name" value="Neuralized"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07525">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00588">
        <property type="entry name" value="NEUZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00253">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00969">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158235">
        <property type="entry name" value="SOCS box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51065">
        <property type="entry name" value="NHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50225">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NHR" evidence="2">
        <location>
            <begin position="3"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="SOCS box" evidence="1">
        <location>
            <begin position="269"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8041595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="305" mass="34695" checksum="3D154ADB3A1593F9" modified="2023-09-13" version="1">MPMFRFHKNHGVNVKLLCDRQVARRSNSFANAITFSQTELQPGEIFMVEIEEQEFGWSGHLRCGLTQHRPSCMVPIRVGSTMYDGVPQLVEFSADSHQAQEEKYIPIPQYSMPDLTNLGKAWIFAVTKYPNTKSQGSNNDNSVETEPFIERCGNVVRIGRNILPLHFLVSTELIPDIEDCISNDEGIPHQHGQKIYATSVGSRIGVTYEIRDCKETNEKECYMHLLINGEDLGATRMDGAEIDQPFYATVDVYGTSKQVRIVQVNHLPSLQESCRVAIRTQIPDREINGLPLPPRLKRYLRYEKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HDB3</accession>
    <name>A0A9Q1HDB3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein C19orf12-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_12920</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the C19orf12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HDB3"/>
    <dbReference type="OrthoDB" id="5976774at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033369">
        <property type="entry name" value="C19orf12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31493">
        <property type="entry name" value="NAZO FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31493:SF1">
        <property type="entry name" value="PROTEIN C19ORF12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20721">
        <property type="entry name" value="C19orf12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00029457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8041969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="217" mass="23861" checksum="DD70FE66D8B73195" modified="2023-09-13" version="1">MPVTPEELVRVLAMLAEEEKLRVTVKETLKGGAMAGTGAVIGGMLGGPVGIAVGGAVGGVAGAWMTAGKFRPVHEVLNEMEPHKRAEIYNAFQNILHGLDYNDVNTLVLLATSDMAVRSQLIGGLAGHMTARWYGKGKFREVSEILTSLEREKREELHQKYQPILMELEQDDGTSLHHRIAMDGELRDKMLSTLKTYLTETLKETLQDNHVERGKAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HFA5</accession>
    <name>A0A9Q1HFA5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Protein shisa-4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_06648</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HFA5"/>
    <dbReference type="OrthoDB" id="10042647at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026910">
        <property type="entry name" value="Shisa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053891">
        <property type="entry name" value="Shisa_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31395:SF23">
        <property type="entry name" value="GEO05642P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31395">
        <property type="entry name" value="SHISA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13908">
        <property type="entry name" value="Shisa_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040148085" evidence="7">
        <location>
            <begin position="24"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="79"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="Shisa N-terminal" evidence="8">
        <location>
            <begin position="24"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="117"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="125"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="195"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8047607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="273" mass="29931" checksum="992CB3EC9CEA10DD" modified="2023-09-13" version="1">MERTRLVFALTVLGTWKIDFCEAEYCSGYTDDEGTYHAGFHCPLTGANSPEDIYCCGTEFDKFCCDTQSNYFLDGLDPAAVIGALLAMLIVFVVCVSLCCCCCSCCLWNRRRNNKRSGPVRYNSLDGTVNTTASTSAGPSDVSGMQNHHQPPSPQNLPSTSGPPYNQHMPPQQMPQPMPQPFSTSSSQSYMGPNQPYPPQNSYPPPSKDGYPFEQQGTGYPPNSSPSMQPPPPYHDPHHQGAMGPPYNEDQPLGFTRPSAPPPQESLPYMTKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HGW3</accession>
    <name>A0A9Q1HGW3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">DNA polymerase eta</fullName>
            <ecNumber evidence="5">2.7.7.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="26">RAD30 homolog A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="30" type="ORF">HOLleu_07268</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="30" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="30">Nanhai2018</strain>
            <tissue evidence="30">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="25">Interacts with REV1. Interacts with monoubiquitinated PCNA, but not unmodified PCNA. Interacts with POLI; this interaction targets POLI to the replication machinery. Interacts with PALB2 and BRCA2; the interactions are direct and are required to sustain the recruitment of POLH at blocked replication forks and to stimulate POLH-dependent DNA synthesis on D loop substrates. Interacts (via C-terminus) with TRAIP. Interacts with ubiquitin. Interacts with POLDIP2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DNA polymerase type-Y family.</text>
    </comment>
    <comment type="caution">
        <text evidence="30">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="5"/>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HGW3"/>
    <dbReference type="OrthoDB" id="5723at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005657">
        <property type="term" value="C:replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042276">
        <property type="term" value="P:error-prone translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010225">
        <property type="term" value="P:response to UV-C"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.100:FF:000007">
        <property type="entry name" value="DNA polymerase eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000022">
        <property type="entry name" value="DNA polymerase eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1170.60:FF:000003">
        <property type="entry name" value="DNA polymerase eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000014">
        <property type="entry name" value="Polymerase (DNA directed), eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.100">
        <property type="entry name" value="DNA polymerase, Y-family, little finger domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036775">
        <property type="entry name" value="DNA_pol_Y-fam_lit_finger_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017961">
        <property type="entry name" value="DNA_pol_Y-fam_little_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052230">
        <property type="entry name" value="DNA_polymerase_eta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041298">
        <property type="entry name" value="UBZ3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001126">
        <property type="entry name" value="UmuC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45873">
        <property type="entry name" value="DNA POLYMERASE ETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45873:SF1">
        <property type="entry name" value="DNA POLYMERASE ETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00817">
        <property type="entry name" value="IMS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11799">
        <property type="entry name" value="IMS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21704">
        <property type="entry name" value="POLH-Rev1_HhH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18439">
        <property type="entry name" value="zf_UBZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036603">
        <property type="entry name" value="DPol_eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100879">
        <property type="entry name" value="Lesion bypass DNA polymerase (Y-family), little finger domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50173">
        <property type="entry name" value="UMUC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51907">
        <property type="entry name" value="ZF_UBZ3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0227">DNA damage</keyword>
    <keyword evidence="20" id="KW-0234">DNA repair</keyword>
    <keyword evidence="11" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8" id="KW-0237">DNA synthesis</keyword>
    <keyword evidence="19" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="18" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="7" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="16" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0515">Mutator protein</keyword>
    <keyword evidence="10" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="21" id="KW-0539">Nucleus</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="22" id="KW-0704">Schiff base</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="17" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="15" id="KW-0862">Zinc</keyword>
    <keyword evidence="14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UmuC" evidence="28">
        <location>
            <begin position="7"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="UBZ3-type" evidence="29">
        <location>
            <begin position="682"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="149"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="461"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="715"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="27">
        <location>
            <begin position="157"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="27">
        <location>
            <begin position="173"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="27">
        <location>
            <begin position="470"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="27">
        <location>
            <begin position="488"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="27">
        <location>
            <begin position="532"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="27">
        <location>
            <begin position="627"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="27">
        <location>
            <begin position="719"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="27">
        <location>
            <begin position="759"/>
            <end position="772"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00044975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00064665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00080427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS51907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="EMBL" id="KAJ8044503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="772" mass="85249" checksum="3F972370AD270DB6" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HI87</accession>
    <name>A0A9Q1HI87_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">F-box/WD repeat-containing protein 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_05443</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HI87"/>
    <dbReference type="OrthoDB" id="19711at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010992">
        <property type="term" value="P:ubiquitin recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000715">
        <property type="entry name" value="F-box protein MET30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001203">
        <property type="entry name" value="F-box/WD repeat-containing protein 1A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19849:SF1">
        <property type="entry name" value="F-BOX_WD REPEAT-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19849">
        <property type="entry name" value="PHOSPHOLIPASE A-2-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="F-box" evidence="5">
        <location>
            <begin position="400"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="497"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="539"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="585"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="625"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="665"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="710"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="750"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="13"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="145"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8046680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="784" mass="88316" checksum="619A26A046E97160" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HIB2</accession>
    <name>A0A9Q1HIB2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NACHT, LRR and PYD domains-containing protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_09089</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HIB2"/>
    <dbReference type="OrthoDB" id="10034282at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312">
        <property type="entry name" value="NACHT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312:SF2">
        <property type="entry name" value="NUCLEOTIDE-BINDING OLIGOMERIZATION DOMAIN-CONTAINING PROTEIN 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NACHT" evidence="1">
        <location>
            <begin position="101"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8045961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="544" mass="62467" checksum="48D676F875A39FE5" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HJR4</accession>
    <name>A0A9Q1HJR4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Histamine H4 receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_06707</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HJR4"/>
    <dbReference type="OrthoDB" id="6163051at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00637">
        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45698:SF1">
        <property type="entry name" value="TRACE AMINE-ASSOCIATED RECEPTOR 13C-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45698">
        <property type="entry name" value="TRACE AMINE-ASSOCIATED RECEPTOR 19N-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="32"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="70"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="105"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="149"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="191"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="322"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="356"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="7">
        <location>
            <begin position="47"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="11"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8047656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="401" mass="45034" checksum="D437DDB4FFA88DC4" modified="2023-09-13" version="1">MNTTPGEEENCFTQPSSYSNPLSHLTSETSKFLVIAYVIVGCVAIVGNGLIVVAFVTSKGIKQLHFNSYIFNRAITDFLIGSVYVPLFVLNLLGEHEVIEHGRLVALGATASFSFRNTTIILTMLMTIDRYRLVSDAIKYTRKQSRKKIRIEVALTWLFSVLVAVFYIHLFDLAYIALMNCENNGLLYLDLIITAFITFVLPFSVLLTLNAVVFYKLYVRLKGFSTKGSATSLQLSKVEATDHVVEKHKMESKFREGKSGGQSGVTCTSTCIGVSDATQKSHLAVPNRQRVQCDNGKDHVKPSAKILLSPTLRKLKRAGGQLFLIVFVFFICWLPHHVSFLYVASKNLELDDSLAWTIWLELLTVVNSATNPLLYIITCKRYQKQILYILGFKSNSYRSTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HK55</accession>
    <name>A0A9Q1HK55_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HTH psq-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_00279</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HK55"/>
    <dbReference type="OrthoDB" id="71166at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007889">
        <property type="entry name" value="HTH_Psq"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05225">
        <property type="entry name" value="HTH_psq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH psq-type" evidence="1">
        <location>
            <begin position="23"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8048106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="91" mass="10173" checksum="E62F37B25A832A7D" modified="2023-09-13" version="1">MLRAGKPDKQYKDATLVECKETIKSGKYSVRKASSVYEIPCSTLMDKLSGRTPVHTTQGPSPVLTKAEEKNLVEWIFYMGKIGYGQRESSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HKW8</accession>
    <name>A0A9Q1HKW8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_02488</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HKW8"/>
    <dbReference type="OrthoDB" id="10063366at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF13">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8049650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="149" mass="18215" checksum="4323CCB16D350839" modified="2023-09-13" version="1">MNKEIRLKEMEREMQKEKEEREMQKEKEEREMQKEKEEREMQKEKRDTGGFDVSKHVKFVPKFQEDNVEKFFNHFEKLGEQLKWPRDKWSILIQSNFTGKAQEVYSALSIEDSMDYDKVKKAILQAYELVPEAYRQNLESIVRRIHKHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HLF1</accession>
    <name>A0A9Q1HLF1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_02441</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HLF1"/>
    <dbReference type="OrthoDB" id="10063284at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45749">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45749:SF21">
        <property type="entry name" value="DUF4371 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="23"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8049618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="365" mass="41823" checksum="2548E9DCFCF01AD7" modified="2023-09-13" version="1">MGPHRPDTDRPKGKSGKRKRSKIQCEECGDTFDDDYRLKHNQKYHMDMIKSHTHIKYKMANAPRNPFEAAKKKPYRQEAQTSTAISAEPTTEGTIMESVTTGDLIQESGDDKDGNYHQIVQLLGRHVPILRRWLSDKRMNPYHVTYLSPQSQKELISLLETELRQRVVDEVHAAWIFSVMADTTPDESPTDLLSVVVSHYNVYEKHAKQSKNFVENLQKVDVNVLDAAALVEATMKVLDQIRSNDKTMTDHIEASCNFAKQISIDPERQFSRHHHQRKVPRRLDDRPDTSAHLKLHMFCGQQFKEVLDVHISRMREHLVQFQDPLKPLFSCLKPPIDASEAPSIAAIFPPTHAPDPLALMAELQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HLK5</accession>
    <name>A0A9Q1HLK5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_02887</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HLK5"/>
    <dbReference type="OrthoDB" id="10066002at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31025">
        <property type="entry name" value="SI:CH211-196P9.1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31025:SF9">
        <property type="entry name" value="SI:DKEY-286J15.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8049921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="100" mass="11469" checksum="AEDD39979A41546E" modified="2023-09-13" version="1">MLQHGRKKVQPFILMLEKRCSPSQVFVIVDGLAIKASTLLKGVDICCKSFYVFDVEYPKECQSTWEFIQKFFFKLGQGKEKSVTSPGVRSLRTFIEKPDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HLP9</accession>
    <name>A0A9Q1HLP9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_03192</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HLP9"/>
    <dbReference type="OrthoDB" id="10030726at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8050116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="163" mass="18739" checksum="28FF038FCE182239" modified="2023-09-13" version="1">MKHASYLVGQELVKCQARQADYFNSKSRFQPYNIGDLVWIDDPAKQRDKLAARWNGPFRVSRVMNNGTTYQLQDLSNLNKPAKILHYNRLKPFCSPYNLGEGDGNVNIPMQVQVPRLNLQMEIPEDQSEPQAPTTNVSKDNKVTRLGRKVKLPSYLNDYELDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0Y8T6</accession>
    <name>A0A9Q0Y8T6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_44492</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000934">
        <property type="protein sequence ID" value="KAJ8017848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0Y8T6"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8017848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="154" mass="18724" checksum="F88C71C36044EF70" modified="2023-09-13" version="1">MPFNLHKFVASPSVEELDSLKKSEIVKVAKHYGIEFQPLMRKDEIKRYVLEYLVDEGVLPSTVLETAITVPTDNTFELKRLEIEMNKEIRLKEMEREREREEREERKEKEEREMQMQIQKEKEEREMQMQMQREKEAREHEFRLKQLELGVIKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0Y9Y1</accession>
    <name>A0A9Q0Y9Y1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_44964</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010001520">
        <property type="protein sequence ID" value="KAJ8017546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0Y9Y1"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8017546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="88" mass="9698" checksum="2A0A3CC4FC569397" modified="2023-09-13" version="1">MPHIEKKNPIVFGGGQRSPGVTGGQTLKTLLTQYLEMRYGMRKNPIIFGGGERSSGVTGGQTLKTSLTRYLKKGNCDSSQTWYIDVPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YA63</accession>
    <name>A0A9Q0YA63_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tyr recombinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_44739</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010001170">
        <property type="protein sequence ID" value="KAJ8017681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YA63"/>
    <dbReference type="OrthoDB" id="8954815at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="entry name" value="Intergrase catalytic core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35617:SF3">
        <property type="entry name" value="CORE-BINDING (CB) DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35617">
        <property type="entry name" value="PHAGE_INTEGRASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="202"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="213"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="228"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8017681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="595" mass="66842" checksum="F9D37433E4DF4A76" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YAJ7</accession>
    <name>A0A9Q0YAJ7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_44328</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000799">
        <property type="protein sequence ID" value="KAJ8017951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="7491853at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01644">
        <property type="entry name" value="RT_pepA17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005312">
        <property type="entry name" value="DUF1759"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040676">
        <property type="entry name" value="DUF5641"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008042">
        <property type="entry name" value="Retrotrans_Pao"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331:SF1">
        <property type="entry name" value="GAG-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03564">
        <property type="entry name" value="DUF1759"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18701">
        <property type="entry name" value="DUF5641"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05380">
        <property type="entry name" value="Peptidase_A17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="2">
        <location>
            <begin position="1418"/>
            <end position="1605"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="100"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="408"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8017951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1739" mass="198617" checksum="2EE05BF31232635B" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YAV7</accession>
    <name>A0A9Q0YAV7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_44472</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000912">
        <property type="protein sequence ID" value="KAJ8017865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YAV7"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8017865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="110" mass="11436" checksum="113E66B6ADBC58BB" modified="2023-09-13" version="1">MIAVIIGKSSPTHSLRSHVGIGSKLQDLEGELVISVRTSSSVVGSKDARTSPVNRQSLDIASATTSLSPISTTMPSTLSAKKSPNLFSRCSMDLLYGLAGLYQPTSPSSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YEN3</accession>
    <name>A0A9Q0YEN3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Metalloendopeptidase</fullName>
            <ecNumber evidence="12">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_38671</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Metalloprotease.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11 12">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11 12">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="12"/>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YEN3"/>
    <dbReference type="OrthoDB" id="291007at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04280">
        <property type="entry name" value="ZnMc_astacin_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000015">
        <property type="entry name" value="Meprin A subunit"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034035">
        <property type="entry name" value="Astacin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001506">
        <property type="entry name" value="Peptidase_M12A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003582">
        <property type="entry name" value="ShKT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10127">
        <property type="entry name" value="DISCOIDIN, CUB, EGF, LAMININ , AND ZINC METALLOPROTEASE DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10127:SF883">
        <property type="entry name" value="ZINC METALLOPROTEINASE NAS-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01400">
        <property type="entry name" value="Astacin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01549">
        <property type="entry name" value="ShK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00480">
        <property type="entry name" value="ASTACIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00254">
        <property type="entry name" value="ShKT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51864">
        <property type="entry name" value="ASTACIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 11 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 11 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 11 12" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2 11 12" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <keyword evidence="6 11 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040539030" description="Metalloendopeptidase" evidence="12">
        <location>
            <begin position="22"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M12A" evidence="13">
        <location>
            <begin position="110"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M12A" evidence="13">
        <location>
            <begin position="437"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="202"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="529"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="528"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="532"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="538"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ8021458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="685" mass="78907" checksum="2646360C7DFD3BDC" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YHJ8</accession>
    <name>A0A9Q0YHJ8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_40304</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YHJ8"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8020654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="51" mass="5965" checksum="E25DC676B01EA8E9" modified="2023-09-13" version="1">MEPTAFVLGAQHKQWSRENKPVSHNWKKGRDRLDLNQLVGNWDVKPHLTNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YHP6</accession>
    <name>A0A9Q0YHP6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">MAM and LDL-receptor class A domain-containing protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_37701</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YHP6"/>
    <dbReference type="OrthoDB" id="10063783at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06263">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.4100">
        <property type="entry name" value="Zona pellucida, ZP-C domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.3210">
        <property type="entry name" value="Zona pellucida, ZP-N domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000998">
        <property type="entry name" value="MAM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051560">
        <property type="entry name" value="MAM_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055355">
        <property type="entry name" value="ZP-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042235">
        <property type="entry name" value="ZP-C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055356">
        <property type="entry name" value="ZP-N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001507">
        <property type="entry name" value="ZP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23282">
        <property type="entry name" value="APICAL ENDOSOMAL GLYCOPROTEIN PRECURSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23282:SF101">
        <property type="entry name" value="MAM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00629">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00100">
        <property type="entry name" value="Zona_pellucida"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23344">
        <property type="entry name" value="ZP-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00020">
        <property type="entry name" value="MAMDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00137">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00241">
        <property type="entry name" value="ZP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50060">
        <property type="entry name" value="MAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51034">
        <property type="entry name" value="ZP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MAM" evidence="2">
        <location>
            <begin position="16"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="ZP" evidence="3">
        <location>
            <begin position="198"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8022720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="387" mass="43295" checksum="1D66507DA2367565" modified="2023-09-13" version="1">MPQLSNQVVILSSDNITCTFEDGTCGFYDLVDDDFDWTRHQGSTLSTDTGPSGDHTTGTGYYMYVETSSGTINWKARLASASYVGTGNQGACLSFYYHMFGSSIGTLKVIYQTVYNTQKQEIVTLSGDHGNMWLQESVHFSSATSDWLVIFEAIRGSSYTGDIAIDDVSISQGPCPLQAEWTTPQDHTYPNEEDVVLRCASSSFQVDIPKNLIEGTYIASDFYLSGDPDNSECKGFYNGAWYISLNSSLTECGTQFNENGSQLHYQNMVEIDPSDGNSVVDFLGVEIPITCEYNSSKTLQTHFKTLNDVIRKQEKGSFEFDFAMYTDITYSARYYSYPVETKLNEELYFRAELLRSASNLEIQLRSCRATPSRDYDDAIVYEFISAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YI56</accession>
    <name>A0A9Q0YI56_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_39264</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YI56"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8021926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="182" mass="21211" checksum="633774A9EDBFA63C" modified="2023-09-13" version="1">MRSLLQNKRVPFSVLVCVIILDEVVTRGDVLWTAHKTVGGAQAPERDSLYEDFEISEEDVEYIRSNLRKEESVVDEVYKVLNLGLRVREQRTRRYLLRKLFERDENLARSFIERLFKEMNEKGKYSRIVHGKETCQSPPGNDMTEIKRDFDFINCSKLFQTVAPVRGSKVQGVFPTKCSEMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YI94</accession>
    <name>A0A9Q0YI94_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Solute carrier family 15 member 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_38119</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Proton-dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8023047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YI94"/>
    <dbReference type="OrthoDB" id="8904098at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000109">
        <property type="entry name" value="POT_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11654">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00854">
        <property type="entry name" value="PTR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0571">Peptide transport</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="85"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="113"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="148"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="188"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="216"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="302"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="340"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="383"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="471"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="513"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="14"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8023047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="572" mass="63371" checksum="EFD28FE858CBACC6" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YIL6</accession>
    <name>A0A9Q0YIL6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_40709</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YIL6"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040157201" description="Secreted protein" evidence="1">
        <location>
            <begin position="17"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8020967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="75" mass="8900" checksum="4CEF5B16C4EE3752" modified="2023-09-13" version="1">MRRLLLLYMLRKSCSAVTQGYVNSIECEKEFYFAESINLPMIYVHLEEMHYPFKCKRGETEHISGINLAYARSLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YIW9</accession>
    <name>A0A9Q0YIW9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Neurabin-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HOLleu_41241</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Nanhai2018</strain>
            <tissue evidence="16">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Synapse</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8019591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="62701at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031175">
        <property type="term" value="P:neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06790">
        <property type="entry name" value="PDZ_neurabin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09512">
        <property type="entry name" value="SAM_Neurabin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000010">
        <property type="entry name" value="Neurabin-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040645">
        <property type="entry name" value="Neurabin-1/2_PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043446">
        <property type="entry name" value="Neurabin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16154">
        <property type="entry name" value="NEURABIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16154:SF6">
        <property type="entry name" value="SPINOPHILIN, ISOFORM J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17817">
        <property type="entry name" value="PDZ_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
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</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
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</entry>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="284"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="26"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="249"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="299"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="26"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="261"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8022469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="432" mass="49109" checksum="390E822959EF7AD5" modified="2023-09-13" version="1">MAEFQSYITMGEDLGLKGKELIDFAKGRDAQAREDKQRERDERQKEREHQRDLTEYELRIAEENAKSRSSGKTDVDPSKASPFKKSEIPCFNEKTDEIDSYLKRFERYAIATEKPKSDWAMLLSTLLTGKALTVYSRLSDDDYKNYNTLKKALLFTYELNADGFRRKFRGSKPEGGETYAQFVERITGYLKRWVDLSNTDKSFEGMLDLMVRDQFYGVCSKELLVFVKEGQPTSVKDMAARADRYVEARGKHLDKKGSTKPGPSNKLQSEQQADKLREWNKNKKCYNCSELGHVSRDCKKPKGYNASKGRNDKAAGLADESDYTPEVKERAAACRTDAEVLVGEDKLRSGLPKVSAVEINQECMPVREGYIGSSKVSVLRDTGCSTVVARKQFVDNSMFTGINRRCVLLDGTVRDVPVAIVFYRYAILSRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YKZ4</accession>
    <name>A0A9Q0YKZ4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Integrase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_40400</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YKZ4"/>
    <dbReference type="OrthoDB" id="10066543at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331:SF1">
        <property type="entry name" value="GAG-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase zinc-binding" evidence="1">
        <location>
            <begin position="98"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF17921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8020731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="287" mass="31829" checksum="DE8F0350EFFECEF6" modified="2023-09-13" version="1">MRRSKGELNEFQELTPKMLDESEVAIVKDAQRKVYSDEIKALAKGNTVYKGSPLFRHTPVLSSDGVIRIGGRLSKSDLAYNTKHPMVLPRVSQDAVLIAQAAHTAVGHLERNSVLAKLREKFWIYGGNQLANSVARNCVFCRRYQAKPCERVMADLPANRVEAALSPFTHCGMDYFGPLIVRRGRSEVKRYGVIFTCFSSRAVHFEVAHSLETDACINAVRQCLARRGPVKSITSDNGTNLVGIVVSGVTGRRIYGSWSLEGSGNCLGENSLLLFHFSKKYVRDYSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YLW5</accession>
    <name>A0A9Q0YLW5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phospholipid scramblase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_38423</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">May mediate accelerated ATP-independent bidirectional transbilayer migration of phospholipids upon binding calcium ions that results in a loss of phospholipid asymmetry in the plasma membrane.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the phospholipid scramblase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YLW5"/>
    <dbReference type="OrthoDB" id="10041953at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017128">
        <property type="term" value="F:phospholipid scramblase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005552">
        <property type="entry name" value="Scramblase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23248:SF63">
        <property type="entry name" value="PHOSPHOLIPID SCRAMBLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23248">
        <property type="entry name" value="PHOSPHOLIPID SCRAMBLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03803">
        <property type="entry name" value="Scramblase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0564">Palmitate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU363116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8021271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="260" mass="29677" checksum="05CF95464FA5409C" modified="2023-09-13" version="1">MTDSEKEAIIEQPGKKGPEWMPKPEVPGCPPGLEYLTQIDQIIVNQKVELLEAFTDFDCKNKYVLKNALGQQVYYAYEGSSNYPESGFCMRLCCSQYRSFVMHIVDNTGQEVIRVHRPFQCWAGCCWCIKGDNCCAYRITVESPPGTPVGYFHQDFSWWKPRYILQDEDRTNILQTEGPCCPCQCCCGCTGDLNFRFSTMNGQDAGNISKQWAGVVKEIFTKADNFNVSFPMDMNVKQKATMIGALFLIDFMYFEEEDNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YM59</accession>
    <name>A0A9Q0YM59_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HOLleu_35039</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Nanhai2018</strain>
            <tissue evidence="17">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YM59"/>
    <dbReference type="OrthoDB" id="8693905at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06610">
        <property type="entry name" value="STKc_OSR1_SPAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000043">
        <property type="entry name" value="serine/threonine-protein kinase OSR1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000114">
        <property type="entry name" value="serine/threonine-protein kinase OSR1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000068">
        <property type="entry name" value="STE20/SPS1-related proline-alanine-rich protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024678">
        <property type="entry name" value="Kinase_OSR1/WNK_CCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050629">
        <property type="entry name" value="STE20/SPS1-PAK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012:SF16">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012">
        <property type="entry name" value="STERILE20-LIKE KINASE, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12202">
        <property type="entry name" value="OSR1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="10 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="29"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="323"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="349"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ8024969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="514" mass="56863" checksum="8134B78C1D58573A" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YMM0</accession>
    <name>A0A9Q0YMM0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">UDP-D-xylose:L-fucose alpha-1,3-D-xylosyltransferase 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_38909</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 77 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YMM0"/>
    <dbReference type="OrthoDB" id="1712432at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005069">
        <property type="entry name" value="Nucl-diP-sugar_transferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052636">
        <property type="entry name" value="UDP-D-xylose:L-fucose_XylT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47032">
        <property type="entry name" value="UDP-D-XYLOSE:L-FUCOSE ALPHA-1,3-D-XYLOSYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47032:SF1">
        <property type="entry name" value="UDP-D-XYLOSE:L-FUCOSE ALPHA-1,3-D-XYLOSYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03407">
        <property type="entry name" value="Nucleotid_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="30"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleotide-diphospho-sugar transferase" evidence="3">
        <location>
            <begin position="119"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8021646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="320" mass="37510" checksum="34DEA05AB380FDD8" modified="2023-09-13" version="1">MNAFTSIQGLLELFTTKMNAIFRLRYRRSTVAYTTAIVLIIVTTYYLTKNEKVNIFGAKLPPFQGKGRIRSEATAKNRTMEDLLPFLTSPVVVTTANYGYLQIVNNFLQSFKKIQVNVSIVIVCEDELLYNHFRERKDVHAVITEFSANITKAQKFRTRKFNEIVNRRIFYILQLIDNGIDTLYLDSDTIWIHNPFPYFDEEYDLFVQQEIFQPYCSGMFYIKATQASLRMISYWHALNKDKYTNQYAYNTAIKYTKGLRVKGLPTDRFVGGEIYFRKTTPWFERKPQPVIVHATWLLGPTEKIKQLKKCGLWFVNASYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YPR4</accession>
    <name>A0A9Q0YPR4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP-dependent DNA helicase</fullName>
            <ecNumber evidence="1">5.6.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_34068</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.3" evidence="1"/>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8026272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YPR4"/>
    <dbReference type="OrthoDB" id="416437at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010285">
        <property type="entry name" value="DNA_helicase_pif1-like_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051055">
        <property type="entry name" value="PIF1_helicase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47642">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47642:SF5">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05970">
        <property type="entry name" value="PIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0227">DNA damage</keyword>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="1" id="KW-0234">DNA repair</keyword>
    <keyword evidence="1" id="KW-0347">Helicase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA helicase Pif1-like DEAD-box helicase" evidence="2">
        <location>
            <begin position="61"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8026272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="172" mass="19383" checksum="7B654B3A0537C067" modified="2023-09-13" version="1">MEIENQSIDVEPEEQQDAILELPTYNDGSRDQNEQHGIMLSLETLHLPFTQEAITTMLRGLNIEQRNLFSHVHKWTKQKIHDNTTKSLHIFLTGGAGTGKSQLIKCITHESRKLFTRIAESPDDITVLLVAFTGTAAFNINGQTIHSALNIFSTSLPYKPLGEDQLNTLRMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YRW3</accession>
    <name>A0A9Q0YRW3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_37030</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8024316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YRW3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8024316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="54" mass="5984" checksum="96CE343B09C64DE8" modified="2023-09-13" version="1">MLNILVSQTEVRGNARTLPAERIYVDILTLLRTSTGPTKPFLISLANVITFHST</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YSW1</accession>
    <name>A0A9Q0YSW1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Actin, cytoskeletal</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_33786</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the actin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8026052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YSW1"/>
    <dbReference type="OrthoDB" id="10249208at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10224">
        <property type="entry name" value="ASKHA_NBD_actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000131">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000291">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000047">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000058">
        <property type="entry name" value="Putative actin-related protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020902">
        <property type="entry name" value="Actin/actin-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004001">
        <property type="entry name" value="Actin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00190">
        <property type="entry name" value="ACTIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00406">
        <property type="entry name" value="ACTINS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00432">
        <property type="entry name" value="ACTINS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01132">
        <property type="entry name" value="ACTINS_ACT_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8026052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="389" mass="43438" checksum="C5F8B1AC0B8ADAA6" modified="2023-09-13" version="1">MCEEEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQTLEFLHIFSFPFQIMFETFNAPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANSVLSGGTSMYPGIADRMQKEITALAPPTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1B989</accession>
    <name>A0A9Q1B989_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Pre-mRNA-splicing factor SYF1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_44670</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the crooked-neck family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010001101">
        <property type="protein sequence ID" value="KAJ8017720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1B989"/>
    <dbReference type="OrthoDB" id="10067343at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071007">
        <property type="term" value="C:U2-type catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000349">
        <property type="term" value="P:generation of catalytic spliceosome for first transesterification step"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000220">
        <property type="entry name" value="Pre-mRNA-splicing factor SYF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055433">
        <property type="entry name" value="HAT_Syf1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056350">
        <property type="entry name" value="HAT_Syf1_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045075">
        <property type="entry name" value="Syf1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11246">
        <property type="entry name" value="PRE-MRNA SPLICING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11246:SF5">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR SYF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23233">
        <property type="entry name" value="HAT_Syf1_CNRKL1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23220">
        <property type="entry name" value="HAT_Syf1_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Pre-mRNA-splicing factor Syf1-like N-terminal HAT-repeats" evidence="9">
        <location>
            <begin position="28"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="Pre-mRNA-splicing factor SYF1 central HAT repeats" evidence="8">
        <location>
            <begin position="189"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF23220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8017720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="235" mass="28028" checksum="355B0CA408E32FE9" modified="2023-09-13" version="1">MSVVSLISTQTLPKKPEIGGKKVMFEEDDLPYEEEILRNPYSVKCWLRYIEHKNDGPVTSLNMIYERALKELPGSYKLWYNYLRLRRKQVKGRVITDPGYEDVNNAFERALVFMHKMPRIWLDYCQFLIDQCKITRTRRTFDRALRALPITQHHRIWPMYLKFVRSHPLHETAVRAYRRYLKLSPEDAEEYVEYLVSIDRMDEAALRMADIVNQENFVSKEGKSNHQVSDEVHLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BAX3</accession>
    <name>A0A9Q1BAX3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Solute carrier family 35 member G1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_41747</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8019955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BAX3"/>
    <dbReference type="OrthoDB" id="306876at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22911">
        <property type="entry name" value="ACYL-MALONYL CONDENSING ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22911:SF6">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER G1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="24"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="54"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="115"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="139"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="177"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="212"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="245"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="272"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="298"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="22"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="330"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="334"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8019955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="358" mass="39497" checksum="91CE8B8EA9EB888E" modified="2023-09-13" version="1">MESEIGIEEHRNGVLHTLCQHKGLFIAFVASCFYATQSFSVSLLQGALPLGELIFLRAVVILMGSIVCLIYLGNPVFSYSRREIFSVLVYSLLGLTSTITLFYAYQNMRAGDAAAIYYGNVAFTGLFARLILKEAFGFVELILVAMTLVGMCLIARPPFLFTAVDDYNTNDITGSQVIPALMAFVSCISLALSVVIVRAVTKDKAMHPFKVTFFYSALSIIVFTVALPTPLMQQWIVPDCAGPRFLILLMTTSSFLGYGLFTFAFSIENALYISLVTMNELFIICILDIAILGLNVQYLSVIGILLIFGSSMLISIKKIIAFKRQKRTSERQNSEMQPVATTTAIMQDGGQRKSSQDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BDJ2</accession>
    <name>A0A9Q1BDJ2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sulfotransferase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_38406</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BDJ2"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005331">
        <property type="entry name" value="Sulfotransferase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03567">
        <property type="entry name" value="Sulfotransfer_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8021259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="218" mass="24817" checksum="8F49467060A46183" modified="2023-09-13" version="1">MKGCLEGWHLEVPREANNHQGAAQFCRSNNGSGHWQCAPGWRQLPVDPFCVRSSSPSKPADRMSTIRQPEYAPVSDDRKLLFIHVPKAGGTSIETSFLFEEQREKLGGHYLGGHHKITEFDQVFFENYHKFGIVRHPCSRLISVWGYYSEGLGNTEDKKWVDGFMDNVTHSSFVAFVERTLYPVGPVHIDEQAHLQTQVEMIFYENGQFGLDQSVTDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BE47</accession>
    <name>A0A9Q1BE47_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_34350</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BE47"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8024441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="196" mass="21985" checksum="FEFE5139CA3E01C5" modified="2023-09-13" version="1">MVLNAYSSGKERLYFQCLETKVLVTIIVTFVVLLCVAETADFPVKGTNLVPSIPCSYVIAACIRPLGDVLLSVLFEVTAIKRDAIATDLLKKFWSVYCSLSPSLSNDLYSDNTARLDQVQNRIYQNCIYCTVGYKMFCLRQCKHRLLRRRHVAGRRGCGWGWIPRWGSRVGVEGVDGDGFLVGVVLSLLLTIIFCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BEW7</accession>
    <name>A0A9Q1BEW7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_36708</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8024080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BEW7"/>
    <dbReference type="OrthoDB" id="10619173at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="72"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="42"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8024080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="192" mass="20658" checksum="3B47D9815DA9CE6D" modified="2023-09-13" version="1">MDQKVSFDLIRLTGLLGFHYESKDSQTWGRIGAVVDTLACNVQALLKSYQEIQDQNLQLKQQLRSCRCKRSRSRDNICGGKDKKSKRQLTGNTYSSSMDVAAPESQTYSNASNNFTSFSLSTCGVSGGNCRGSSGTGQGGSNGAAANVEAEAKQVLLRNGFALLDQIGHGSFGLVYSARPLDGNIRSIYPVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BFA4</accession>
    <name>A0A9Q1BFA4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Sperm-tail PG-rich repeat-containing protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_38152</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8023074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BFA4"/>
    <dbReference type="OrthoDB" id="406368at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051291">
        <property type="entry name" value="CIMAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010736">
        <property type="entry name" value="SHIPPO-rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21580">
        <property type="entry name" value="SHIPPO-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21580:SF60">
        <property type="entry name" value="SPERM-TAIL PG-RICH REPEAT-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07004">
        <property type="entry name" value="SHIPPO-rpt"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="154"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="303"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="389"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="167"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="389"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="422"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="438"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8023074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="587" mass="64499" checksum="87B0182E1023220D" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BFW6</accession>
    <name>A0A9Q1BFW6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_34000</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8026211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BFW6"/>
    <dbReference type="OrthoDB" id="6092537at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15489">
        <property type="entry name" value="PHD_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37445">
        <property type="entry name" value="PROTEIN CBG24663"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37445:SF3">
        <property type="entry name" value="ZINC FINGER PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="6">
        <location>
            <begin position="25"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="102"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8026211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="306" mass="35070" checksum="3A73BF1ED85342A3" modified="2023-09-13" version="1">MSTPKLSTSESGLLLGHESSGAEEIWLCKLCDKEFSGGKCELLECEYCGKHFCRKCLGLSAAEYKLFAKRSDLHWYCPPCEEKAVKSIRIEKEIEERCKDYFSKFEKRLNDLEEKISQKTSVEDVQNLIKESEKGGACLENVQTAVNEQLTEFRESEQRKLNVIIFNAKESDKRDGEARKEEDFKLLDKLCKVMKSDIEAIKNVTRLGKRVVKEGGGVERRPMRVIFEDTKSKSKFLSNLGNLASASEDLRFLSVAQDMTPKEREINKKKFAEAKQKNEELGSGDFKFVVRGPPWERKVMKVKVKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BFZ0</accession>
    <name>A0A9Q1BFZ0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Cytochrome b reductase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_35250</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8025130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BFZ0"/>
    <dbReference type="OrthoDB" id="907479at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043205">
        <property type="entry name" value="CYB561/CYBRD1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006593">
        <property type="entry name" value="Cyt_b561/ferric_Rdtase_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10106">
        <property type="entry name" value="CYTOCHROME B561-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10106:SF0">
        <property type="entry name" value="LD36721P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03188">
        <property type="entry name" value="Cytochrom_B561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00665">
        <property type="entry name" value="B561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50939">
        <property type="entry name" value="CYTOCHROME_B561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="4" id="KW-0349">Heme</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="49"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="84"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="124"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="157"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="183"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="222"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b561" evidence="12">
        <location>
            <begin position="13"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8025130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="278" mass="31311" checksum="06BC5E3611ACE7FB" modified="2023-09-13" version="1">MSSSKIFWSLIVSSQLCGLTAYALMQSWLHKYNDGFDWNSEVSHLIQNYHALFMLLGFLLLFGEATLTYRLLSRVKAPYLLQKAVHATILIIATILWVVGLVAAFKARNLDQSHGKETKHFYSFHSWLGLILMVIFVSQFLWFAYSVRISSVPPTKLCFAIVWYAHAKFISPALQALYQSIHIFNGLFLFVLATATCFTGLNEVGIYKVENYKLNSSSGIHLNMVGIFALGFVTTVGYLLWKPEFKLTPAVGEEPKESANSNCTDEAITLRNSDKTVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BGG8</accession>
    <name>A0A9Q1BGG8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_31737</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BGG8"/>
    <dbReference type="OrthoDB" id="775972at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33198">
        <property type="entry name" value="ANK_REP_REGION DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33198:SF20">
        <property type="entry name" value="RETROTRANSPOSON GAG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8026796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="128" mass="14844" checksum="4B9B1D623C86CEAB" modified="2023-09-13" version="1">MTLLQIAGPETVELFKTFLWRNNADKDDPDLIMDQLEKYCNPRKNTTYERHIFNKTNQNPFESIHSYVTDSKVKAKTCEFGNLTDSLIRDRIVCGMLRDALRARLLREEDLKLEKAFNISRSGEASDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BHJ3</accession>
    <name>A0A9Q1BHJ3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Protein atonal-like 8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_34015</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus speckle</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8026223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BHJ3"/>
    <dbReference type="OrthoDB" id="10001938at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070888">
        <property type="term" value="F:E-box binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11421">
        <property type="entry name" value="bHLH_TS_ATOH8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000052">
        <property type="entry name" value="Protein atonal homolog 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032660">
        <property type="entry name" value="ATOH8_bHLH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050359">
        <property type="entry name" value="bHLH_transcription_factors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19290">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX PROTEIN NEUROGENIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19290:SF102">
        <property type="entry name" value="TRANSCRIPTION FACTOR ATOH8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="8">
        <location>
            <begin position="268"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="13"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="29"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8026223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="432" mass="49615" checksum="F12371E638911169" modified="2023-09-13" version="1">MEVAEDLTIKSSTKSHTRKTGSRKKRRKQSEPKRLSLKFDDFSDKTGPSWSSKHISEEEPPIFAKDDSDGDSGILSLNPHEVREAPFSISRSSSEIIQTDFSEEETHFLSSDVDEVCTGSSYVIPSQEVSIVEDEDTQERTSLDGRDAERDVISTYVEKKDALSHVNWQHKIFRTNDSYGRSRKGHVYEAARHEQPISFIKSTNLPENEFDTIRFERSGPQSSLVRPTTEQTGVPANHEINNNNALRRTSKKTNMQHVAHGHKGLNRSRRIVANARERNRVHTISAAFEGLRRAVPCYSHNQKLSKLAILRIACSYILALANLADLDYSPNQKKLSFSECVDLCTMTLQAEGRSKRKKVTQWRTEDFHNGGHERVLPTTWEPLPPLRQLVSEILEKRAFFHNDSFIERYSRESHSQARQQWVFVMVRKFPHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BIH5</accession>
    <name>A0A9Q1BIH5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Afadin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_32312</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6260541at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050839">
        <property type="term" value="F:cell adhesion molecule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032880">
        <property type="term" value="P:regulation of protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22711">
        <property type="entry name" value="FHA_AFDN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15471">
        <property type="entry name" value="Myo5p-like_CBD_afadin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028842">
        <property type="entry name" value="Afadin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037977">
        <property type="entry name" value="CBD_Afadin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002710">
        <property type="entry name" value="Dilute_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000159">
        <property type="entry name" value="RA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
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    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
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        <property type="entry name" value="AFADIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10398:SF2">
        <property type="entry name" value="AFADIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="DIL"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00314">
        <property type="entry name" value="RA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="DILUTE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="RA"/>
        <property type="match status" value="2"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ras-associating" evidence="3">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-associating" evidence="3">
        <location>
            <begin position="183"/>
            <end position="290"/>
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    </feature>
    <feature type="domain" description="Dilute" evidence="4">
        <location>
            <begin position="535"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="2">
        <location>
            <begin position="932"/>
            <end position="1018"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="881"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1028"/>
            <end position="2003"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2058"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="118"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="881"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="917"/>
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        </location>
    </feature>
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        <location>
            <begin position="1070"/>
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        </location>
    </feature>
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        <location>
            <begin position="1224"/>
            <end position="1238"/>
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            <begin position="1263"/>
            <end position="1285"/>
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        <location>
            <begin position="1317"/>
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        <location>
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            <dbReference type="PROSITE" id="PS50106"/>
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        </source>
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    </evidence>
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            <dbReference type="EMBL" id="KAJ8027229.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="2090" mass="236254" checksum="7ECCE7D23B57854D" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BJN0</accession>
    <name>A0A9Q1BJN0_HOLLE</name>
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        <submittedName>
            <fullName evidence="4">Carbonic anhydrase-related protein 10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_29923</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
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        <lineage>
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            <taxon>Metazoa</taxon>
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        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
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        </source>
    </reference>
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        <text evidence="1">Belongs to the alpha-carbonic anhydrase family.</text>
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        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
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        </location>
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            <begin position="20"/>
            <end position="333"/>
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            <begin position="27"/>
            <end position="308"/>
        </location>
    </feature>
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            <dbReference type="ARBA" id="ARBA00010718"/>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAJ8027851.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
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    </evidence>
    <sequence length="333" mass="37909" checksum="E3762046B338B974" modified="2023-09-13" version="1">MDSVTDFLIFSFLLVLSKGDEEVGWDDKWSYHGLNGPDYWGLMHSEWTRCHNGKFQSPINLVSKRLIYDAHLGLFEFDYEDLGELGGNYTNTGRAPVFTPRPEDAATFTVRGGPLHYEYQIHDLWIHFGENENEGSDHLIEGNAFQGEIQITFWNKGLYNSYKEAEVSPRGLAAIAIFLQKGNKTSDMKSELAKLTRANVLSSIVYKGSSVGIPDLNVLKLMPDTTEYITYEGSLTSPPCYETVTWIVLNKPIHISEMELSNIRELRREEEMTTVEVGDTQQAVQTRLLESNFRGVKPRGNRAIRTNIGHVQNLDEKCYQNRIEHTYKASVLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BJW0</accession>
    <name>A0A9Q1BJW0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Neuromedin-B receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_30067</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8027962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BJW0"/>
    <dbReference type="OrthoDB" id="9996086at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45695:SF9">
        <property type="entry name" value="LEUCOKININ RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45695">
        <property type="entry name" value="LEUCOKININ RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="46"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="81"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="160"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="215"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="258"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="300"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="9">
        <location>
            <begin position="61"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8027962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="380" mass="43021" checksum="6315033AD7F82A64" modified="2023-09-13" version="1">MAHTEQNYFLHPTEIEAFNNISTEVTGAKDCLAKELLTAEKHSSTAVLIISIIVSIVGIAGNGSLVYLFCREKNLRDVNNAFITNLAIGDFFFITVFTPAKIFQHFFFFFPFGKIFCYIQIFAHYCTQDVSMFSLVALSFIRFRVVVFPMHIQTNHHRKLLIRVTCVAIWTVALVSSVLPASRCAEIKVCSFIKVHLVEGFNDTTMELFFTLRFFLVYLIPLVMITFLYSAMAMILWRSSKILQGSVSTNSHSSGRRRLAIVVLVLIAVFAVCWMPNYIALYIEYYTKASVPPFVSNSRFVLSCISSAVNPIILYLTSSTYRSYLRQHVCLHPFQRFGSFTKTSRKTQTTSRSVRSGLKQSAIRGEKIGAKSRDAALTVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BJW9</accession>
    <name>A0A9Q1BJW9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Pogo transposable element with KRAB domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_30133</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BJW9"/>
    <dbReference type="OrthoDB" id="10043687at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15517">
        <property type="entry name" value="PHD_TCF19_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050863">
        <property type="entry name" value="CenT-Element_Derived"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004875">
        <property type="entry name" value="DDE_SF_endonuclease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007889">
        <property type="entry name" value="HTH_Psq"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19303:SF74">
        <property type="entry name" value="POGO TRANSPOSABLE ELEMENT WITH KRAB DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19303">
        <property type="entry name" value="TRANSPOSON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03184">
        <property type="entry name" value="DDE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05225">
        <property type="entry name" value="HTH_psq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH psq-type" evidence="3">
        <location>
            <begin position="31"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="DDE-1" evidence="2">
        <location>
            <begin position="221"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="474"/>
            <end position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8028012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="564" mass="64351" checksum="01860B47AC7D3D88" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BKQ7</accession>
    <name>A0A9Q1BKQ7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Krueppel-like factor 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_30675</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BKQ7"/>
    <dbReference type="OrthoDB" id="4748970at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000021">
        <property type="entry name" value="Basic krueppel-like factor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000018">
        <property type="entry name" value="Krueppel-like factor 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000875">
        <property type="entry name" value="zinc finger protein 236 isoform X7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1110.10">
        <property type="entry name" value="AN1-like Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035896">
        <property type="entry name" value="AN1-like_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF120">
        <property type="entry name" value="KRUPPEL-LIKE FACTOR 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118310">
        <property type="entry name" value="AN1-like Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="233"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="263"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="293"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="122"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="170"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="170"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="217"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8028447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="315" mass="35577" checksum="4662BCF8F43C9FA0" modified="2023-09-13" version="1">MATSVSHTPEAVWAIAQSSEDSSHDNGDVETDEKNKYCDYEQCVEKDQVYFACDQCQKTYCDKHVQSKCHPCIEMCSGYIKTEMEEGESSGPSNHHIGLLSRAETSGVVHIEPYQTEPVDLSINGRNRKRKSNSPKRIINAHGSSSTTHYPAADSVNLHKLVSSTAIISIPKPPTISSTSSHNRVSMDHVQHQSSPRFAQADPVSSYPSQVWQGRPRTREPRSRTADPRKRSHQCTFVGCGKMYTKSSHLKAHLRTHTGEKPYKCNWEGCGWSFARSDELTRHYRKHTGAKPFKCPHCERAFSRSDHLSLHMKRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BM72</accession>
    <name>A0A9Q1BM72_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Secretory carrier-associated membrane protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_28538</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BM72"/>
    <dbReference type="OrthoDB" id="1924787at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055038">
        <property type="term" value="C:recycling endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032588">
        <property type="term" value="C:trans-Golgi network membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007273">
        <property type="entry name" value="SCAMP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10687:SF2">
        <property type="entry name" value="SECRETORY CARRIER-ASSOCIATED MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10687">
        <property type="entry name" value="SECRETORY CARRIER-ASSOCIATED MEMBRANE PROTEIN SCAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8029200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="87" mass="9071" checksum="CBFDEA55BA60111B" modified="2023-09-13" version="1">MATSKSPGQKVIAGLMLIIALCFTLIFVAKVIYLQQVHSLYRSTGASFEKAQQEFATGVVSNPGVQSATKQAASAAVSSAFSSGNQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BMD9</accession>
    <name>A0A9Q1BMD9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Peroxisomal multifunctional enzyme type 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_28719</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; fatty acid beta-oxidation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BMD9"/>
    <dbReference type="OrthoDB" id="3592703at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018812">
        <property type="term" value="F:3-hydroxyacyl-CoA dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03448">
        <property type="entry name" value="HDE_HSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05353">
        <property type="entry name" value="hydroxyacyl-CoA-like_DH_SDR_c-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.129.10:FF:000013">
        <property type="entry name" value="Peroxisomal multifunctional enzyme type 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000185">
        <property type="entry name" value="peroxisomal multifunctional enzyme type 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.4290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1050.10">
        <property type="entry name" value="SCP2 sterol-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057326">
        <property type="entry name" value="KR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002539">
        <property type="entry name" value="MaoC-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054357">
        <property type="entry name" value="MFE-2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051687">
        <property type="entry name" value="Peroxisomal_Beta-Oxidation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003033">
        <property type="entry name" value="SCP2_sterol-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036527">
        <property type="entry name" value="SCP2_sterol-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45024">
        <property type="entry name" value="DEHYDROGENASES, SHORT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45024:SF2">
        <property type="entry name" value="SCP2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01575">
        <property type="entry name" value="MaoC_dehydratas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22622">
        <property type="entry name" value="MFE-2_hydrat-2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02036">
        <property type="entry name" value="SCP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00822">
        <property type="entry name" value="PKS_KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55718">
        <property type="entry name" value="SCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ketoreductase" evidence="12">
        <location>
            <begin position="17"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="476"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00073497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8029347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="738" mass="78993" checksum="7C9CCB95EFA23400" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BNV6</accession>
    <name>A0A9Q1BNV6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">RNA polymerase-associated protein CTR9-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_29664</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8030086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BNV6"/>
    <dbReference type="OrthoDB" id="343875at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016593">
        <property type="term" value="C:Cdc73/Paf1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000069">
        <property type="entry name" value="CTR9 homolog, Paf1/RNA polymerase II complex component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000077">
        <property type="entry name" value="CTR9 homolog, Paf1/RNA polymerase II complex component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000084">
        <property type="entry name" value="CTR9 homolog, Paf1/RNA polymerase II complex component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000089">
        <property type="entry name" value="CTR9 homolog, Paf1/RNA polymerase II complex component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000162">
        <property type="entry name" value="CTR9 homolog, Paf1/RNA polymerase II complex component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056836">
        <property type="entry name" value="ARM_TT21_4th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031101">
        <property type="entry name" value="Ctr9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006597">
        <property type="entry name" value="Sel1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013105">
        <property type="entry name" value="TPR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14027">
        <property type="entry name" value="RNA POLYMERASE-ASSOCIATED PROTEIN CTR9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14027:SF2">
        <property type="entry name" value="RNA POLYMERASE-ASSOCIATED PROTEIN CTR9 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25068">
        <property type="entry name" value="ARM_TT21_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13374">
        <property type="entry name" value="TPR_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07719">
        <property type="entry name" value="TPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13174">
        <property type="entry name" value="TPR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00671">
        <property type="entry name" value="SEL1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="166"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="Tetratricopeptide repeat protein 21A/21B fourth ARM" evidence="5">
        <location>
            <begin position="171"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="201"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="344"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="454"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="534"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="871"/>
            <end position="1185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="871"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="926"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="967"/>
            <end position="976"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="977"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="987"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1014"/>
            <end position="1032"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1097"/>
            <end position="1114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1130"/>
            <end position="1148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="1159"/>
            <end position="1173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF25068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8030086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1185" mass="135402" checksum="B1C4E2E4E352A543" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BPX6</accession>
    <name>A0A9Q1BPX6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_26966</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BPX6"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040281256" evidence="1">
        <location>
            <begin position="20"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8030519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="169" mass="19208" checksum="603FAF044AC6871A" modified="2023-09-13" version="1">MLGRILLVILPLLFVFVASEENDDRECLCEMYWARKVDPGPKTVTFRIDLFEIISPSFKNDKYDDCETFSGKCLQKCEDHIRGLYDAISLPTDRRFKLGKMLCEQIGVGISRFAPRHIFLKTKIDGCEVFDNVEMGDLCCYSCVNSGTWEVSYNPGCSSTNFPIGDCDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BQ92</accession>
    <name>A0A9Q1BQ92_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Latrophilin-like protein LAT-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_27104</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BQ92"/>
    <dbReference type="OrthoDB" id="6134459at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15039">
        <property type="entry name" value="7tmB3_Methuselah-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053231">
        <property type="entry name" value="GPCR_LN-TM7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45902">
        <property type="entry name" value="LATROPHILIN RECEPTOR-LIKE PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45902:SF1">
        <property type="entry name" value="LATROPHILIN RECEPTOR-LIKE PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00249">
        <property type="entry name" value="GPCRSECRETIN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="404"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="435"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="472"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="513"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="561"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="608"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="635"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="7">
        <location>
            <begin position="403"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="675"/>
            <end position="701"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8030636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="713" mass="79494" checksum="2CF3F3B822DE2797" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BRG0</accession>
    <name>A0A9Q1BRG0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_27963</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8031280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BRG0"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8031280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="68" mass="7577" checksum="A2DF213D436B8866" modified="2023-09-13" version="1">MSNQGSENSLIISSAEENMTSNDCDTKSKQTLELETSEETKNKTSDDCDLNLKELPESESSKEVQVFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BS56</accession>
    <name>A0A9Q1BS56_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_24857</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BS56"/>
    <dbReference type="OrthoDB" id="10607236at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="131"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8031611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="200" mass="22627" checksum="2A111CFC38A2253F" modified="2023-09-13" version="1">MAGVYNTLKDRPLVFILFQINGTKTFRRINVLQNMTCTNKTFNNFPCTELNIIALNMLSTLASSKLLEDAKDMIKHCNPGFVEDPARTLSAMIKTPTDDEISNVKNFFSSDTVAPQPIHLQPKIQKGPGKVIGIVFLFVVVIASLVITGWLIRTRIFNSHSRTTNFSNVLYSRIDYDHENLDDYSDDDDDKGELSMFERA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BTN8</accession>
    <name>A0A9Q1BTN8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_26129</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BTN8"/>
    <dbReference type="OrthoDB" id="5948273at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912">
        <property type="entry name" value="IP13529P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912:SF34">
        <property type="entry name" value="NOTOCHORD-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8032572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="483" mass="48998" checksum="F232FA165BE97B8B" modified="2023-09-13" version="1">MIVADNLGSHGIGGFNESFSGFRICRFCMCTSQELANPNLLRKFVERTDSNHIKISRRVVTDDQMKTTYGIRQNSPFNSLKYFHVANGLPSDFAHDLFEGVCPHVLGKVLTHYVVADMISIQACNDTISNFPYMLTDKSNKPSPLMWSNGCVIVKQKAAQMWCLMRLLFLLWGNIIPQGDIYWQLLIYLVEICDLAAAPVHSNESIAYLEGTVFDFLDLFVMLFQVKKLKPKMHYLMHYPKQIRSFGPIVQLLDSKIRAKHSFFKQIVRTTQNKKNYLLFTLTKRHQLSTSSWGGGGVGGGVGGGGWGLGWGGRGWGGWWAGWRVGGGGGGGGAGWGGWGAGGGGGGGGGGGVGGWGGGGGGGWGGGGVGGGGGGGGGGWVGGGVGGRGCVGGGVGGGGGGGGGGWGGGGGGGGGGGGGGGWGGGGVGGGGGGGGGGWGGGGVGGGGGGGGGGWGGGGVGYQNVPLFKRRGFEHRFSTPKHVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BXH9</accession>
    <name>A0A9Q1BXH9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_21289</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BXH9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8034445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="89" mass="9783" checksum="DCADB5F4580D7BA9" modified="2023-09-13" version="1">MADICELVEFLETRNPFSDAQSLRSIATDADGRVTVDTAKDVGEGILDSMVGKSVLEHTFRKKDQAVTLNTSVIKINNDTVNIDPQLLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BZB8</accession>
    <name>A0A9Q1BZB8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Death domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_20077</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BZB8"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01670">
        <property type="entry name" value="Death"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00174">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.220.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000488">
        <property type="entry name" value="Death_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000906">
        <property type="entry name" value="ZU5_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00531">
        <property type="entry name" value="Death"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00791">
        <property type="entry name" value="ZU5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00005">
        <property type="entry name" value="DEATH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50017">
        <property type="entry name" value="DEATH_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51145">
        <property type="entry name" value="ZU5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Death" evidence="2">
        <location>
            <begin position="326"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="Death" evidence="2">
        <location>
            <begin position="489"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="domain" description="ZU5" evidence="3">
        <location>
            <begin position="689"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="217"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="405"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="574"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="226"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="421"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8036183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="798" mass="91637" checksum="4D3630F07392A3A9" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BZS9</accession>
    <name>A0A9Q1BZS9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">CREB/ATF bZIP transcription factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_19346</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8035615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BZS9"/>
    <dbReference type="OrthoDB" id="6606299at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BZIP" evidence="3">
        <location>
            <begin position="185"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="68"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="120"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="270"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="204"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="70"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="133"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="281"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8035615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="322" mass="36043" checksum="C1D91340723C1BDA" modified="2023-09-13" version="1">MDESYFDFDSLHWQAGDFNLEGADLTEMGDLLAESLQESQLPFGKEGMGQKHSDIPLEHSYAFSAKKVPLSSQSESLTNTQNGDQGNKIGLFENDTNVLSTKDDNWVDELLNVKKKDDSVSCLPLKSDPEPLQQPSSPCTQKPTQSSVEAIPPESTIPSTSKKSRKRKASGHSTESDAGEMVLDRNTKNAIAARENRLKKKQYVEGLEKKNKELEKGNEIMQDKTEEQAERISHLEREVEYLKNVLHNQSALSSLLGSLDPDTSVRLSVAGQGGKWRRVGGSKETDERQKKEAGMKIFRTVRKEEMDVITFVQAREGKWGQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BZW3</accession>
    <name>A0A9Q1BZW3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Vacuolar protein sorting-associated protein 13C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_19327</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the VPS13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8035599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="428159at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045053">
        <property type="term" value="P:protein retention in Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026847">
        <property type="entry name" value="VPS13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056748">
        <property type="entry name" value="VPS13-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056747">
        <property type="entry name" value="VPS13-like_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009543">
        <property type="entry name" value="VPS13_VAB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16166:SF93">
        <property type="entry name" value="INTERMEMBRANE LIPID TRANSFER PROTEIN VPS13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16166">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN VPS13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25037">
        <property type="entry name" value="VPS13_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25033">
        <property type="entry name" value="VPS13_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25036">
        <property type="entry name" value="VPS13_VAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VPS13-like middle region" evidence="3">
        <location>
            <begin position="8"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="Vacuolar protein sorting-associated protein 13 VPS13 adaptor binding" evidence="4">
        <location>
            <begin position="489"/>
            <end position="1029"/>
        </location>
    </feature>
    <feature type="domain" description="Intermembrane lipid transfer protein VPS13-like C-terminal" evidence="5">
        <location>
            <begin position="1621"/>
            <end position="1728"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="32"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="51"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF25037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8035599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1777" mass="197000" checksum="875123115D087D82" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C0C8</accession>
    <name>A0A9Q1C0C8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Malate dehydrogenase 1B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_20142</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C0C8"/>
    <dbReference type="OrthoDB" id="4069699at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.110.10">
        <property type="entry name" value="Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022383">
        <property type="entry name" value="Lactate/malate_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015955">
        <property type="entry name" value="Lactate_DH/Glyco_Ohase_4_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02866">
        <property type="entry name" value="Ldh_1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56327">
        <property type="entry name" value="LDH C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactate/malate dehydrogenase C-terminal" evidence="2">
        <location>
            <begin position="26"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="138"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8036227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="159" mass="18080" checksum="94BBB159CEAA566E" modified="2023-09-13" version="1">MLALCQGYPNYVRRSYHNRALSHLLGWYQVPEGLFFSFPVRFQKGSWRLVQDLNVTDELREELSKIVDELEREIEVIFPSPNKVKKVADSQEDATGENKGEKVFGQPDRDSTADTKNSPLTPDGDNNEATRNVTLSRIIEESETDTREGTTVTETDKEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C168</accession>
    <name>A0A9Q1C168_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Cyclin-T2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HOLleu_20240</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Nanhai2018</strain>
            <tissue evidence="15">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cyclin family. Cyclin C subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C168"/>
    <dbReference type="OrthoDB" id="25002at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20538">
        <property type="entry name" value="CYCLIN_CCNT_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22249">
        <property type="entry name" value="UDM1_RNF168_RNF169-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000004">
        <property type="entry name" value="Cyclin T2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000009">
        <property type="entry name" value="cyclin-T2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043198">
        <property type="entry name" value="Cyclin/Ssn8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10026">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21797">
        <property type="entry name" value="CycT2-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="8 12" id="KW-0195">Cyclin</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Cyclin-like" evidence="14">
        <location>
            <begin position="39"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="259"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="307"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="660"/>
            <end position="982"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="994"/>
            <end position="1163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1184"/>
            <end position="1214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="353"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="406"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="419"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="434"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="490"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="527"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="588"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="669"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="682"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="736"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="765"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="791"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="812"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="837"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="948"/>
            <end position="964"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="969"/>
            <end position="982"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="994"/>
            <end position="1010"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1013"/>
            <end position="1032"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="1039"/>
            <end position="1055"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="1081"/>
            <end position="1095"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="1118"/>
            <end position="1146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1149"/>
            <end position="1159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1185"/>
            <end position="1203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ8036305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1214" mass="135598" checksum="A78941827E1F5DE8" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C1C7</accession>
    <name>A0A9Q1C1C7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Receptor-type guanylate cyclase gcy-8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_20255</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C1C7"/>
    <dbReference type="OrthoDB" id="1890790at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017046">
        <property type="term" value="F:peptide hormone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052612">
        <property type="entry name" value="ANP_Clearance_Receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44755">
        <property type="entry name" value="NATRIURETIC PEPTIDE RECEPTOR 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44755:SF8">
        <property type="entry name" value="RECEPTOR LIGAND BINDING REGION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040405256" evidence="5">
        <location>
            <begin position="19"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="Receptor ligand binding region" evidence="6">
        <location>
            <begin position="67"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8036319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="235" mass="26586" checksum="DD4FA0774CF20643" modified="2023-09-13" version="1">MAIMMRILIATFLVFASADCFYKPDITVALILNDRDFRLDETYLKPPVDIALSKVDELIETGVYVNFSLSYIFEVTTNTCSEAGKNNAAGRSAELYFNRKIVAFLGPPCNIHTVSTADLAAFWNVPVIVGAGTSGNLEDKTRFPRFRSVAIAKFFRKIFRHFRWTRCAVMVARGGFHFVITAPDMLKVFQATNINSVTFYEKDFQSSEKMLDQASQQARSKWSTLKHVQVKVYST</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C1V2</accession>
    <name>A0A9Q1C1V2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Zinc transporter 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_20653</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C1V2"/>
    <dbReference type="OrthoDB" id="9944568at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010043">
        <property type="term" value="P:response to zinc ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1510.10:FF:000027">
        <property type="entry name" value="Zinc transporter ttm-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="entry name" value="Cation efflux protein transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027470">
        <property type="entry name" value="Cation_efflux_CTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036837">
        <property type="entry name" value="Cation_efflux_CTD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058533">
        <property type="entry name" value="Cation_efflux_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050681">
        <property type="entry name" value="CDF/SLC30A"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01297">
        <property type="entry name" value="CDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11562">
        <property type="entry name" value="CATION EFFLUX PROTEIN/ ZINC TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11562:SF17">
        <property type="entry name" value="RE54080P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16916">
        <property type="entry name" value="ZT_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160240">
        <property type="entry name" value="Cation efflux protein cytoplasmic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="Cation efflux protein transmembrane domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0864">Zinc transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="98"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="128"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="160"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="199"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="258"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="288"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein transmembrane" evidence="10">
        <location>
            <begin position="99"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein cytoplasmic" evidence="11">
        <location>
            <begin position="321"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF16916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8036624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="411" mass="45021" checksum="B268C0F428B55A13" modified="2023-09-13" version="1">MNGFLNNNNHDQSCNCQKCSTIVTDVEFSHSKVAKSDQMVPAVSACEGDTVLHTVDDSDDVPMLDVCAANIEDDFCQCHPSKSIKLVQSSNKNAQTKLLIVSFVCFLFMVAELTGGILSGSLAIMTDAAHLFSDFTGFVISLFALWFARKPATTRMSFGFYRAEIIGAVISVLIIWAITLILIYLAVMRCIKRDYEIDADIMLITAGCGVFINIILILILQCVGHSGHGHSHSYVPGNNHSRSKDGPRREGKNLNVRAAFIHCVGDLLQSVGVLVAAMIIKFKPEYKLADPICTFVFSVIVLCTTVSILRDALMVLMEAVPKNVDYKDLRNELEKLPGVCMAHSLHVWSLTTTCSALAVHLAVEPGTETQPVLTAASDLLSKKFGFSFTTIQVEYYIEDAMSSCRRCQGPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C2Q5</accession>
    <name>A0A9Q1C2Q5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_18349</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C2Q5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8037517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="50" mass="5809" checksum="7C8C26A90631AAFE" modified="2023-09-13" version="1">MIVPIIIYQVLLTCVLQRFLILINMPATELCLQIRSDIKTQPIFLDCHSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C377</accession>
    <name>A0A9Q1C377_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_18059</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C377"/>
    <dbReference type="OrthoDB" id="10042460at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="95"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="142"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8037279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="201" mass="23714" checksum="28A4BD3ED523B91A" modified="2023-09-13" version="1">MNSCMRCVLFLTFTEPQEEDAYTRAVLTFIVGLVMLETWGIFVYILYIVRFSFQRHFTLLLSFIEEHEGEIDVCRGVLSEVFSDFSCFRRFSHTYTLVMFPIAFIAMASNLSWEYLMYKACRNNEDVAKIQTPITALARSEIVMGIGLYITAMGAVNIMYLWDNFFRDVRRLHSERYDEFWRKMILCMSVVSNNETNFFLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C409</accession>
    <name>A0A9Q1C409_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Prokineticin receptor 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_16406</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C409"/>
    <dbReference type="OrthoDB" id="10053194at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042923">
        <property type="term" value="F:neuropeptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004983">
        <property type="term" value="F:neuropeptide Y receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000611">
        <property type="entry name" value="NPY_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24235:SF29">
        <property type="entry name" value="GH23382P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24235">
        <property type="entry name" value="NEUROPEPTIDE Y RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01012">
        <property type="entry name" value="NRPEPTIDEYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="44"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="85"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="121"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="64"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="181"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="233"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="240"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8038853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="256" mass="29364" checksum="72465D0D331CC305" modified="2023-09-13" version="1">MESPNFTYNLPSFEIDPYDYNGILGPNSYGISTNVESVNSSVSIIAKIVLAITYTLVMVICGVGNLLLCCVVLRFRRMRTTTNILIGNLALSDFFVAILCVPFNFYYYMTDSWPFGKPMCVIVGYLKMTSFYVSVNSLLAIAIDRLYHHHAPLETPDDNLHRRYRHLFYMVCVAECRHSHRRPHKNGGVQRRDRSVHGDGPFQSAEGLHNGALLCRVSHPAFRHDRRLLSDSEETLVSSHSRRSRDRRTRTCRRDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C581</accession>
    <name>A0A9Q1C581_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">TNF receptor-associated factor 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_15789</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C581"/>
    <dbReference type="OrthoDB" id="6499288at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005164">
        <property type="term" value="F:tumor necrosis factor receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043122">
        <property type="term" value="P:regulation of canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.210.10:FF:000001">
        <property type="entry name" value="TNF receptor-associated factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.210.10">
        <property type="entry name" value="Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002083">
        <property type="entry name" value="MATH/TRAF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008974">
        <property type="entry name" value="TRAF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049342">
        <property type="entry name" value="TRAF1-6_MATH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10131:SF138">
        <property type="entry name" value="RE66324P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10131">
        <property type="entry name" value="TNF RECEPTOR ASSOCIATED FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21355">
        <property type="entry name" value="TRAF-mep_MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00061">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50144">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="4 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="MATH" evidence="6">
        <location>
            <begin position="107"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="42"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8038379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="258" mass="29293" checksum="6F71C6F3383EE7CE" modified="2023-09-13" version="1">MIQKLIPTAKLATIQEILPIVERNSRALASEHERSKIFWEALVKLIDMYKTLEEDNKKLSSDLARMVESSRTKDVDLHQLQGRISVLDTTITRLKMSVEQLENVSYNGVLIIEVPNYSHKKAAAMTGTPSFYSDDFYTDRHGYRMCARIYLNGDGVGKGSHLSIFFAIKKGPYDNYLSWPFQRRVSFTLLNQAGKDHLSDAFRPDPTSSSFQKPVKEMNIASGCPMFVAHKVIENPVEGFLRNNTICLRVKVDPSTSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C8M4</accession>
    <name>A0A9Q1C8M4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Guanine nucleotide exchange factor MSS4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_12083</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C8M4"/>
    <dbReference type="OrthoDB" id="30840at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006892">
        <property type="term" value="P:post-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.150.10:FF:000005">
        <property type="entry name" value="Guanine nucleotide exchange factor MSS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.10">
        <property type="entry name" value="Metal Binding Protein, Guanine Nucleotide Exchange Factor, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007515">
        <property type="entry name" value="Mss4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011057">
        <property type="entry name" value="Mss4-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011323">
        <property type="entry name" value="Mss4/transl-control_tumour"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13276">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR MSS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13276:SF0">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR MSS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04421">
        <property type="entry name" value="Mss4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51316">
        <property type="entry name" value="Mss4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51796">
        <property type="entry name" value="MSS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8041298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="135" mass="15032" checksum="FC048A81F42CFF2F" modified="2023-09-13" version="1">MAFAGSEPEAKNSEMGVLSMDGLLEDGKNAKYLKCERCRSTILRPGDATHTKKEQIFLPHMKKKSENQAAGEGETLSDFWLVKDMFTFENVGFTNTVDNKKFLICADCEVGPIGWQDLADSVNLYVALARVVYES</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CA58</accession>
    <name>A0A9Q1CA58_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_11940</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CA58"/>
    <dbReference type="OrthoDB" id="10064525at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000882">
        <property type="entry name" value="Predicted gene, 21060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000679">
        <property type="entry name" value="Zinc finger protein 341"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000618">
        <property type="entry name" value="zinc finger protein 341 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050636">
        <property type="entry name" value="C2H2-ZF_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47772:SF13">
        <property type="entry name" value="GASTRULA ZINC FINGER PROTEIN XLCGF49.1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47772">
        <property type="entry name" value="ZINC FINGER PROTEIN 200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="46"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="299"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="327"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="406"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="434"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="464"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="501"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="527"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="555"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="583"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="611"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="644"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="672"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="260"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="689"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="270"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="689"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="707"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="733"/>
            <end position="747"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8041197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="865" mass="97048" checksum="633DB3C5A577386D" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CBD9</accession>
    <name>A0A9Q1CBD9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_12505</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CBD9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8041633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="85" mass="10389" checksum="DC3B5ED2F3496698" modified="2023-09-13" version="1">MMAFENFNCLGQDWWEEPGRRDYSCRMLIENRILETPSDVRKDVSHYKEVKLKFTFSSRKSSFPIKRYRKEPKTTACYKKKRKNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CBM5</accession>
    <name>A0A9Q1CBM5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Kinesin-like protein KIF15</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_12793</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Spindle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. KIN-12 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01373">
        <property type="entry name" value="KISc_KLP2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000034">
        <property type="entry name" value="Kinesin family member 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044986">
        <property type="entry name" value="KIF15/KIN-12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37739">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIN-12D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37739:SF8">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIN-12D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7 10" id="KW-0505">Motor protein</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="13">
        <location>
            <begin position="19"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="394"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="560"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="675"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1388"/>
            <end position="1410"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="362"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="511"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="585"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="721"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="855"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="946"/>
            <end position="1384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="408"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="570"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="677"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="100"/>
            <end position="107"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ8041871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1410" mass="161309" checksum="C2EE39D6405AF3A1" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CC86</accession>
    <name>A0A9Q1CC86_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">peptide-methionine (S)-S-oxide reductase</fullName>
            <ecNumber evidence="2">1.8.4.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Peptide-methionine (S)-S-oxide reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_13255</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MsrA Met sulfoxide reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.8.4.11" evidence="2"/>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8042246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CC86"/>
    <dbReference type="OrthoDB" id="77405at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008113">
        <property type="term" value="F:peptide-methionine (S)-S-oxide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1060.10">
        <property type="entry name" value="Peptide methionine sulphoxide reductase MsrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002569">
        <property type="entry name" value="Met_Sox_Rdtase_MsrA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036509">
        <property type="entry name" value="Met_Sox_Rdtase_MsrA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049006">
        <property type="entry name" value="MsrA_helical"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43774">
        <property type="entry name" value="PEPTIDE METHIONINE SULFOXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43774:SF1">
        <property type="entry name" value="PEPTIDE METHIONINE SULFOXIDE REDUCTASE MSRA 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20939">
        <property type="entry name" value="MsrA_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01625">
        <property type="entry name" value="PMSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55068">
        <property type="entry name" value="Peptide methionine sulfoxide reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptide methionine sulphoxide reductase MsrA" evidence="5">
        <location>
            <begin position="2"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="Selenoprotein methionine sulfoxide reductase A helical" evidence="6">
        <location>
            <begin position="83"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00030643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF20939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8042246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="134" mass="15649" checksum="EAA87ACEBF0299CA" modified="2023-09-13" version="1">MFWHNHDSTVCHKRQYMSAIFYHGDEQKALAEKTKDEHQKTLKKKIQTLIKPAEEFYDAEDYHQKYMLRQHRSLFHSMNFAPKELIKSHAAARLNGYVAGFGKRSNFEEEVDKLGLSDEQANYVRGVLGRGGMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CDF4</accession>
    <name>A0A9Q1CDF4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_09793</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8042910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CDF4"/>
    <dbReference type="OrthoDB" id="8799554at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="83"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8042910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="317" mass="36788" checksum="9D250171FCA43C0C" modified="2023-09-13" version="1">MTSPRGHSGSRALGSIKLKGTVFPPLARTVNDSSRQYFVEHQLMVQGDSMGLSGFVSKSSERVDLSPTKSPSRPVTSRPDAVSLKLAEEVKEMQNKEKQYKNKIQSLQKVIEQLKQDVKIRDSDLAAINQKLQEIEEHYKVLYNDECSSHTETKSKLKVVSDELEEKNELVEELKRKHEESMQEVKNRYETQVKQLEDDMKREITERDDKIEKMKKQMANFLKDNSWERQQQLEELTKELGKVSEEAIDLKAKLKSLMKNKKCENCDHLKNDIRKRDQKISENEATIKELMVLCQKFETQLKQQAILQECAEMLDKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CDP5</accession>
    <name>A0A9Q1CDP5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Sphingosine 1-phosphate receptor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_10095</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CDP5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00637">
        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24246:SF27">
        <property type="entry name" value="ADENOSINE RECEPTOR, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24246">
        <property type="entry name" value="OLFACTORY RECEPTOR AND ADENOSINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="64"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="108"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="142"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="187"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="12"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8043132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="300" mass="33999" checksum="FC2D4DB13608A4A4" modified="2023-09-13" version="1">MRIGICCLALVINMIVFVLQIKVKSFLRTQYVFAVTLTIADIIHAAALVGETYLFRQNIFPIPIWYGAFAFASGLQSYLSILAVAYDRYLALCAIPLKYKLIITIRKYWVVILIMNAFCFTCGFVFFGLVSHQSVALTYTSFVYPIALMSAICIVYIRLAYLVSKSLKTLNFTVDIQRRRARQTKRLMIAFATILLAHVICNMPQRVFSLYISLQPTGRRYTVFEHVAIGNWLYNVQTINTCVNPVIYWKHVLSKDTKFPFASMMYRAICPVTCKGEPPTCSQDETESKTSSAVSAISAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CE93</accession>
    <name>A0A9Q1CE93_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_11152</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CE93"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8043863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="117" mass="13089" checksum="2A4FD9C7C403A30A" modified="2023-09-13" version="1">MPHIERKNPIVFGGGQRSSGVTGGRMLKTLLTRYLKMRYGKRKNPIIFDGGQRSSGVTGGRMLKTLLTQYLKVRYGKRKNRIVFDGGQRSSGVTGGQTLKTLLTRYLKIGSLDKFHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CG44</accession>
    <name>A0A9Q1CG44_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">B-related factor 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HOLleu_07421</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Nanhai2018</strain>
            <tissue evidence="15">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TFIIB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CG44"/>
    <dbReference type="OrthoDB" id="511529at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000126">
        <property type="term" value="C:transcription factor TFIIIB complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097550">
        <property type="term" value="C:transcription preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000995">
        <property type="term" value="F:RNA polymerase III general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001006">
        <property type="term" value="F:RNA polymerase III type 3 promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070897">
        <property type="term" value="P:transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20553">
        <property type="entry name" value="CYCLIN_TFIIIB90_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20554">
        <property type="entry name" value="CYCLIN_TFIIIB90_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.10:FF:000012">
        <property type="entry name" value="Putative transcription factor IIIB 90 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000002">
        <property type="entry name" value="Transcription factor IIIB 90 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000007">
        <property type="entry name" value="Transcription factor IIIB 90 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.650">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011665">
        <property type="entry name" value="BRF1_TBP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000812">
        <property type="entry name" value="TFIIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013150">
        <property type="entry name" value="TFIIB_cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013137">
        <property type="entry name" value="Znf_TFIIB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618:SF4">
        <property type="entry name" value="TRANSCRIPTION FACTOR IIIB 90 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07741">
        <property type="entry name" value="BRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00382">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08271">
        <property type="entry name" value="Zn_Ribbon_TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00685">
        <property type="entry name" value="TIFACTORIIB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51134">
        <property type="entry name" value="ZF_TFIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIB-type" evidence="14">
        <location>
            <begin position="3"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="385"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="472"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="528"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="593"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="673"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="404"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="474"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="528"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="547"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="598"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="688"/>
            <end position="709"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ8044632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="709" mass="79059" checksum="F70E51A921EC01F1" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CGT8</accession>
    <name>A0A9Q1CGT8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Leucine-rich repeat-containing protein 15</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_07994</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CGT8"/>
    <dbReference type="OrthoDB" id="676979at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001360">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050328">
        <property type="entry name" value="Dev_Immune_Receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24373:SF370">
        <property type="entry name" value="FISH-LIPS, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24373">
        <property type="entry name" value="SLIT RELATED LEUCINE-RICH REPEAT NEURONAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="446"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8045067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="477" mass="54192" checksum="58E1FEA1549D356F" modified="2023-09-13" version="1">MSHYDVWDSCERPFPSGCCKLGHLFVCNGVNVTHVDNFADEIPQHCTSLTIANANNSFINNGYWKFLSNLTYLEDLAFYNNKISSLSDLKPVYIEDLENLKFLTLAFGELRTFPVEIFHTLASLEYLSLQCNKLQNISEGNWNFPLLNNLIMSHNRIATVGIKHFKGLENLAELDLSYNRISHFSLKVLDSMPNLKYLRLSFNQLVYISDFSHQHAHLLYFALSSNLFVSLEGSVFNGLKSVRTIEFSSNSIKDPPSVNSKNNVTSALALSLESNLIEVLSPLFFDHFPYLNLINVDFNRIHHIEENTFRSVPNVTSISMRGNAIHIIPRQLFQHLQNLNFIELQVNRIQNLDPYIFNQLSKHVQVYIQNNPIVCDCQALPLLKWLSASGMSIMHFPTCQSPKPLENQNIYYATLPQDCPASTLMPVSTNVTPTEHPNTFKDQNTLIFLIGLLLAPLICSCLIPLCAVCRSSHEDSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CHP2</accession>
    <name>A0A9Q1CHP2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Gamma-aminobutyric acid type B receptor subunit 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_07675</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CHP2"/>
    <dbReference type="OrthoDB" id="2150267at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038039">
        <property type="term" value="C:G protein-coupled receptor heterodimeric complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004965">
        <property type="term" value="F:G protein-coupled GABA receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007214">
        <property type="term" value="P:gamma-aminobutyric acid signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15047">
        <property type="entry name" value="7tmC_GABA-B-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002455">
        <property type="entry name" value="GPCR3_GABA-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10519">
        <property type="entry name" value="GABA-B RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10519:SF74">
        <property type="entry name" value="GAMMA-AMINOBUTYRIC ACID TYPE B RECEPTOR SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="111"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="142"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="170"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="10">
        <location>
            <begin position="49"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8044814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="227" mass="25302" checksum="BE4AE43BF95BD505" modified="2023-09-13" version="1">MSSPNLNSIIIIGCILLYVSACMAGSESFGDDPAIFLKLQQVVKDRHLFVMVGAFLFLEVTVLSAYFIISPVRVEKKYLPIKDMDIDSMVILQPYVQYCTSDHQQYWEGALYGYKSLLLVFGVFLAWETRKVNIPALNDSKLIGLCVYNVVLLTGISVGVKLVLTRDPSMSFLFTSGIRIFATTMVLGIIFIPKVRSLKPGRCNHESTISEAVLPWISLNPQPVLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CI57</accession>
    <name>A0A9Q1CI57_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_08381</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CI57"/>
    <dbReference type="OrthoDB" id="10069635at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032055">
        <property type="entry name" value="TMEM72"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28474">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28474:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16054">
        <property type="entry name" value="TMEM72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="90"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="119"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8045381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="167" mass="18885" checksum="74E8CCE7AA3AC35D" modified="2023-09-13" version="1">MADEEIETKTYSGKLPYCRPYICLPILWGSTSGLVLAIVGVYDLFKADDHVVTGIFLLSVGLVVLCLEIFSLLSLTFLRKVHCCLKYISLIGWLETWKRGLVYTLVGIFCFLAVLRTKYSIVCGISMVLAGSLYILKTCRARRLKASTKTDRRILTEDFQEEQNLDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-04-02" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CL03</accession>
    <name>A0A9Q1CL03_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Plexin-A4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_06506</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the plexin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="125363at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002116">
        <property type="term" value="C:semaphorin receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017154">
        <property type="term" value="F:semaphorin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030334">
        <property type="term" value="P:regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00603">
        <property type="entry name" value="IPT_PCSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11236">
        <property type="entry name" value="Sema_plexin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031148">
        <property type="entry name" value="Plexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002165">
        <property type="entry name" value="Plexin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016201">
        <property type="entry name" value="PSI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001627">
        <property type="entry name" value="Semap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036352">
        <property type="entry name" value="Semap_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041019">
        <property type="entry name" value="TIG1_plexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041362">
        <property type="entry name" value="TIG2_plexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22625">
        <property type="entry name" value="PLEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22625:SF70">
        <property type="entry name" value="PLEXIN A, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01437">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01403">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01833">
        <property type="entry name" value="TIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18020">
        <property type="entry name" value="TIG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17960">
        <property type="entry name" value="TIG_plexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00429">
        <property type="entry name" value="IPT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00423">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00630">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103575">
        <property type="entry name" value="Plexin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101912">
        <property type="entry name" value="Sema domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51004">
        <property type="entry name" value="SEMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1238"/>
            <end position="1260"/>
        </location>
    </feature>
    <feature type="domain" description="Sema" evidence="13">
        <location>
            <begin position="32"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1334"/>
            <end position="1392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1410"/>
            <end position="1480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1334"/>
            <end position="1358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1453"/>
            <end position="1469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ8047492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1480" mass="164425" checksum="927CB11C62A7E0F1" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CLG9</accession>
    <name>A0A9Q1CLG9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Sarcospan</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_05689</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CLG9"/>
    <dbReference type="OrthoDB" id="10027693at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016010">
        <property type="term" value="C:dystrophin-associated glycoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042383">
        <property type="term" value="C:sarcolemma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007237">
        <property type="entry name" value="CD20-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030429">
        <property type="entry name" value="Sarcospan"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15260">
        <property type="entry name" value="SARCOSPAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15260:SF1">
        <property type="entry name" value="SARCOSPAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04103">
        <property type="entry name" value="CD20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="86"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="123"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="154"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="239"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="297"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="301"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="312"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8046865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="323" mass="36531" checksum="5657BA092A2CE756" modified="2023-09-13" version="1">MPDYDGSNSSRSTGPAMSFPRTSRSSRSSSLPELRYFHPDQSVSTPLKELSEQGDNGSTQIHFPVSEPMSADSGNRNQKYMLDCCSLPMVLVTLQILVGFTIVVVAITMLLMTPSVWHRDAPYWAGIVLLLTGGIGLYFNAIKRGIYERSRRGVFIKIAFFTLSVVCLFVNSIASAFCGIQTRLVSTILSTSEPEDKPCRRVEKDCICRRTTNNEFIREHVYEDILDCESFLINLRTYMYIQCALNAIGAIASFMAAMLMWRRRYAEFHSGLRFYSYSAALPSRPWEDRHKPHYMDGDKLTNGTIPNGHTSVHTEHLDTTPHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CM74</accession>
    <name>A0A9Q1CM74_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_00382</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CM74"/>
    <dbReference type="OrthoDB" id="10064127at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33198">
        <property type="entry name" value="ANK_REP_REGION DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33198:SF19">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8048182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="161" mass="18314" checksum="098552474402C3E2" modified="2023-09-13" version="1">MATLGILKEFQEGENWTEFTERLEQYFLANDIEDNGKKRAILLTVCGSATYSLMKNLLAPAKPTDKSFSELVTLVKNHHQPKASIIVSRFKFHTSTREPEQLISDYLAQLRKLAEPCEFGNSLEEMLRDAQGSIPERARKVYRPPSQNICGCRCTAQIFQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CMV0</accession>
    <name>A0A9Q1CMV0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_00299</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CMV0"/>
    <dbReference type="OrthoDB" id="10068969at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331:SF6">
        <property type="entry name" value="DOUBLECORTIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8048123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="122" mass="13460" checksum="4EE1A290F134BB1D" modified="2023-09-13" version="1">MADLPTDRMETSSPFTNVGTDVFGPWIIVTRRTRGGSANSKRWAAIFTCLSSRAVHVEVVESMDTSSFIRALRRFMAIRDPVAKLRCDQGTNFIGASSELEGSAGNLDQRRISQYLSSKGIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CMV8</accession>
    <name>A0A9Q1CMV8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">phosphoinositide 5-phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.36</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_00318</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + H2O = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:22764"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58178"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="EC" id="3.1.3.36"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the synaptojanin family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the central section; belongs to the inositol 1,4,5-trisphosphate 5-phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.36" evidence="4"/>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CMV8"/>
    <dbReference type="OrthoDB" id="1925875at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004439">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048488">
        <property type="term" value="P:synaptic vesicle endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09089">
        <property type="entry name" value="INPP5c_Synj"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
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    <dbReference type="InterPro" id="IPR046985">
        <property type="entry name" value="IP5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000300">
        <property type="entry name" value="IPPc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002013">
        <property type="entry name" value="SAC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015047">
        <property type="entry name" value="SYNJ1/2_RRM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11200">
        <property type="entry name" value="INOSITOL 5-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11200:SF257">
        <property type="entry name" value="PHOSPHOINOSITIDE 5-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08952">
        <property type="entry name" value="DUF1866"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22669">
        <property type="entry name" value="Exo_endo_phos2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02383">
        <property type="entry name" value="Syja_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01165">
        <property type="entry name" value="DUF1866"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00128">
        <property type="entry name" value="IPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50275">
        <property type="entry name" value="SAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="120"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1013"/>
            <end position="1179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1027"/>
            <end position="1039"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="1041"/>
            <end position="1055"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="1070"/>
            <end position="1084"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1105"/>
            <end position="1117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="1118"/>
            <end position="1147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1150"/>
            <end position="1164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8048138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1179" mass="133054" checksum="0286D42296A5DCFA" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CNJ1</accession>
    <name>A0A9Q1CNJ1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF4806 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_00850</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="8785518at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
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        <property type="entry name" value="DUF4806"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34153:SF2">
        <property type="entry name" value="SI:CH211-262H13.3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34153">
        <property type="entry name" value="SI:CH211-262H13.3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16064">
        <property type="entry name" value="DUF4806"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4806" evidence="2">
        <location>
            <begin position="271"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="137"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="160"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="174"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8048506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="397" mass="44220" checksum="B8C693EEBC3CC064" modified="2023-09-13" version="1">MILYCNNGLSTLLSACRVLQLLCLLRTTMQFLLYKKFGALERTTVTGLLKKSLLKSERAVRSCEPPSDNWGKHPVRILETTDTYEKGLSLVKRAEDTSDLQSEAENNRSKNSRKQKKRSRYISDIESDKGDDEDEGGYSTSSSLELPSPPTPPAALRMSPTTPTVGPTTPTLPVQLSQCTESSIPGPSTAVSSVPRTPTLPSLQRLKPAVPSPSQPGRNFDMFTAAEKSMLTLIMEVKAELKEVKSHVKHLINIHQSKPTDDEEEDMALQEMSLPCSDVDELFQLDEKIKTSKTIHKQLLSILSTTGGRTLREVVSRMMKELMLNCVAIHFNWTGQGSKHSFRDLALRHVMERAVRRNAATQTTSGHEIKVELCHFLKGAPDRDGGRQARRKPLPDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CP11</accession>
    <name>A0A9Q1CP11_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_00178</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CP11"/>
    <dbReference type="OrthoDB" id="10056877at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="235"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8048024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="256" mass="29202" checksum="C9CAC4DC74AD3FB7" modified="2023-09-13" version="1">MPCSTVDPGFLAAKKLKELRISKVVNVNERQVLQFLGQCRDMLVAATSKLLVKCPLTYTITRNLACMDPCMMATNKDDCVAKFRRVLHKLVFLKQVNEIDCDSLQWEYEAFLDEAVSRNFSKFKGYSCDDQRLDTFLSMYMNGVPAYDKLWQLTKKLLILSHGQATVERGFSVNAQLIVENMKEKSVVFQRVVHNAIANYGGLLKTPVTKSLLSYAASARRKYMAYLEDQNHQRSLQKSFDSKRRSGENTEQLEAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CR57</accession>
    <name>A0A9Q1CR57_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_01670</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CR57"/>
    <dbReference type="OrthoDB" id="416437at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8049089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="381" mass="43060" checksum="445BA00C010CD3DC" modified="2023-09-13" version="1">MNLLDDSMFLTTDDLPSSFVVANNKYSAHYYDSIFGKLQPDQSSSFEALDVVIQQAFTVSSYNILLKGHYMMSLFHFSIGDILFFDSHSRSEAGLVDPDGRAILLQFKSLQSFTQFLIVLCTSLHFEESTLVELKPVSIQLINNDAYGEQYKSSSTVPPSLTCLLKDDFNCDLSILPNNSSNIVHAAKLKRKTEQGIHSVPISSHARKRKRRILNTEVKGTHAKEFSATSVFGSTENHIIANSDPIEPIATDYDVCKQQFQDAIKQGPSFVCTAYLQTWFRHSVVQLKLLKVSDDITTLLAKCWIGYISMYEKEWICRTCVKHIKKGQIPRLSIVNNCHFPPKPPELNIHPLEERLLSPRIPFMQIRELPRGGQLSITGIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CR81</accession>
    <name>A0A9Q1CR81_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_03134</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CR81"/>
    <dbReference type="OrthoDB" id="6153050at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33332">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8050077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="143" mass="16256" checksum="857A1C24A34F72B2" modified="2023-09-13" version="1">MEICTWMKSNLLILNLDKTEVVHFSSRHKKVVKELTSLKIGQSIITPSNSVRKLGVHFECSGNFNVHINHICKSAYFALHRIGKLRTLLDQAATEKLVHAFVTSRLDYCNSLLYGCPESHLDKLQSIQNAAARLLKRQKNLNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CRD0</accession>
    <name>A0A9Q1CRD0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_02956</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CRD0"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8049968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="121" mass="13945" checksum="7B00E6DF2E7CCF34" modified="2023-09-13" version="1">MDHNHNVGRPQAVVQQPRKGSSVKGELQYKKSYSKIQKQWVLKPVYEKKKYDYISPMMAGVVKRKQDAMVEAIEQAPAPNIAPVPAPAKEDMVAKHSSRFEVFIVHSRYRRVCVYFSCYCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CRW2</accession>
    <name>A0A9Q1CRW2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Prenylcysteine oxidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_04331</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the prenylcysteine oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CRW2"/>
    <dbReference type="OrthoDB" id="437369at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001735">
        <property type="term" value="F:prenylcysteine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030327">
        <property type="term" value="P:prenylated protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030328">
        <property type="term" value="P:prenylcysteine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010795">
        <property type="entry name" value="Prenylcys_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017046">
        <property type="entry name" value="Prenylcysteine_Oxase1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15944">
        <property type="entry name" value="FARNESYLCYSTEINE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15944:SF0">
        <property type="entry name" value="PRENYLCYSTEINE LYASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13450">
        <property type="entry name" value="NAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07156">
        <property type="entry name" value="Prenylcys_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040215776" evidence="8">
        <location>
            <begin position="30"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="domain" description="Prenylcysteine lyase" evidence="9">
        <location>
            <begin position="137"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8050942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="508" mass="56443" checksum="C6C62A7135E4F93A" modified="2023-09-13" version="1">MSFNNRIRALYSLSVLSTSAFLLAKITRAEKDEANVRTQQPPSPRIAVVGGGIGGTSSAYFLRELFGEAAQIDVYEPGSIGGRVASILVGDRKYECGASIIHPRNKYMVQHAEKFGLKHRKDVTVLMGSYDGKEFVFEESSWGFITILKMLWQYGWRDLFGLQREVNKMLKSFDSIYDFQENGRCFTTVRDMMQAMGGDDFVRWTMSPADQVLREMKFSELFISQVVSLATRVNYGQEPNMEAFAAFVSLAGVQPGLWAVNGGNELLPQNFLAKSNSTVMNSTVTSITLHASNKLETNSTFSVTSSSEGNSQTHNYDMVIIATPLVSRISSIQFNGFPTPIGPYPGEYRSTVATFAKGTVNKEEFGKSKDEFIPGEILTTQEIPLGIRSVSKTFPIDFKGDSDKDNFVYKVFSTDVPTEKQMSQIFSSYSNDVYRAWLAYPYYRNVDIDTVAPFQLYPGLFYVNAIEWAASAMEMSAIGAKNSALLAKKHWSGGGEESVVVGSQKIEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CRZ9</accession>
    <name>A0A9Q1CRZ9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">NLR family CARD domain-containing protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_04406</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8051002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CRZ9"/>
    <dbReference type="OrthoDB" id="427518at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312">
        <property type="entry name" value="NACHT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312:SF2">
        <property type="entry name" value="NUCLEOTIDE-BINDING OLIGOMERIZATION DOMAIN-CONTAINING PROTEIN 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040438854" evidence="2">
        <location>
            <begin position="24"/>
            <end position="1196"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="349"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1164"/>
            <end position="1196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1164"/>
            <end position="1190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8051002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1196" mass="136394" checksum="5737F339FCA53ACF" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CUF6</accession>
    <name>A0A9Q1CUF6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_04478</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8051054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CUF6"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8051054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="73" mass="8648" checksum="4D3CBAA2914389AD" modified="2023-09-13" version="1">MQGYQQYPRSSLQMPVYSIPVLPRPPLPPVTPPSSQYYPSLIYAFQRMKETFTWDHTPCHMQVQYVSLDLTWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1GWU7</accession>
    <name>A0A9Q1GWU7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Iduronate 2-sulfatase</fullName>
            <ecNumber evidence="15">3.1.6.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Alpha-L-iduronate sulfate sulfatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">HOLleu_30446</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">Nanhai2018</strain>
            <tissue evidence="20">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Lysosomal enzyme involved in the degradation pathway of dermatan sulfate and heparan sulfate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>Hydrolysis of the 2-sulfate groups of the L-iduronate 2-sulfate units of dermatan sulfate, heparan sulfate and heparin.</text>
            <dbReference type="EC" id="3.1.6.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="14">Monomer. The 58-kDa mature form is composed of two chains resulting from proteolitic processing, the 42-kDa chain and the 14-kDa chain that remain stably associated and form the 58-kDa intermediate form which is enzymatically active.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Lysosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the sulfatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.6.13" evidence="15"/>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1GWU7"/>
    <dbReference type="OrthoDB" id="96314at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043202">
        <property type="term" value="C:lysosomal lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004423">
        <property type="term" value="F:iduronate-2-sulfatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901136">
        <property type="term" value="P:carbohydrate derivative catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd16030">
        <property type="entry name" value="iduronate-2-sulfatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.720.10:FF:000027">
        <property type="entry name" value="iduronate 2-sulfatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035874">
        <property type="entry name" value="IDS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024607">
        <property type="entry name" value="Sulfatase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000917">
        <property type="entry name" value="Sulfatase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45953">
        <property type="entry name" value="IDURONATE 2-SULFATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45953:SF1">
        <property type="entry name" value="IDURONATE 2-SULFATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00884">
        <property type="entry name" value="Sulfatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00149">
        <property type="entry name" value="SULFATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0458">Lysosome</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 18" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040129605" description="Iduronate 2-sulfatase" evidence="18">
        <location>
            <begin position="26"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="domain" description="Sulfatase N-terminal" evidence="19">
        <location>
            <begin position="32"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00050460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00056350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00062513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00066413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00068336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00081076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAJ8028257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="579" mass="65591" checksum="851FEBB1689A2DF2" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1GXB4</accession>
    <name>A0A9Q1GXB4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_30788</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1GXB4"/>
    <dbReference type="OrthoDB" id="498125at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="CDD" id="cd09076">
        <property type="entry name" value="L1-EN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8028527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="221" mass="25331" checksum="83D0A15E77EDD1D8" modified="2023-09-13" version="1">MMSAVDAQSGAMKLFFLMAPIKARGWLFFLGQVFHLLVSKKIIDIHGRFIILDVNVNNVIFSLVCVYGPNTDNALFVHTVYNHLESFQCDNIICGGDFNFVFNLTLDKYGGVQNTNFKARDACLSYLDKFSLDDVWRTRNPLSTSFTWTSDIDNIRCRLDFFLISKKLVNKVIDCYFQPPLQSDHSIVILQCDISSEPRGPGYWNFNNSLLLDLLYIELIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1GYX8</accession>
    <name>A0A9Q1GYX8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Lysyl oxidase-like 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_30504</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10063988at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053231">
        <property type="entry name" value="GPCR_LN-TM7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036024">
        <property type="entry name" value="Somatomedin_B-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001212">
        <property type="entry name" value="Somatomedin_B_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45902">
        <property type="entry name" value="LATROPHILIN RECEPTOR-LIKE PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45902:SF1">
        <property type="entry name" value="LATROPHILIN RECEPTOR-LIKE PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01033">
        <property type="entry name" value="Somatomedin_B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00530">
        <property type="entry name" value="SRCR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00201">
        <property type="entry name" value="SO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90188">
        <property type="entry name" value="Somatomedin B domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00524">
        <property type="entry name" value="SMB_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50958">
        <property type="entry name" value="SMB_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1509"/>
            <end position="1532"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1553"/>
            <end position="1571"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1583"/>
            <end position="1603"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1623"/>
            <end position="1644"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1669"/>
            <end position="1695"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1725"/>
            <end position="1745"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1757"/>
            <end position="1778"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="9">
        <location>
            <begin position="304"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="domain" description="SMB" evidence="10">
        <location>
            <begin position="422"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="9">
        <location>
            <begin position="768"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="9">
        <location>
            <begin position="875"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="domain" description="SMB" evidence="10">
        <location>
            <begin position="1001"/>
            <end position="1041"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="8">
        <location>
            <begin position="1513"/>
            <end position="1781"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="345"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="376"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="836"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="924"/>
            <end position="985"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8028304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1808" mass="202932" checksum="282CBEC5085EE71B" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H0F5</accession>
    <name>A0A9Q1H0F5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Kinesin-like protein KIF24</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_28456</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. KIN-13 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007019">
        <property type="term" value="P:microtubule depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01367">
        <property type="entry name" value="KISc_KIF2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000012">
        <property type="entry name" value="Kinesin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47971:SF20">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIF24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47971">
        <property type="entry name" value="KINESIN-RELATED PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="2" id="KW-0493">Microtubule</keyword>
    <keyword evidence="5 8" id="KW-0505">Motor protein</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="10">
        <location>
            <begin position="47"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="380"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="513"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="702"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="801"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1088"/>
            <end position="1115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1142"/>
            <end position="1195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1268"/>
            <end position="1322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1358"/>
            <end position="1450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1473"/>
            <end position="1581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1614"/>
            <end position="1638"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1683"/>
            <end position="1714"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1818"/>
            <end position="1870"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="380"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="455"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="468"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="533"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="711"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="838"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1095"/>
            <end position="1104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1268"/>
            <end position="1278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1359"/>
            <end position="1376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1393"/>
            <end position="1418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1428"/>
            <end position="1443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1512"/>
            <end position="1528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1529"/>
            <end position="1542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1544"/>
            <end position="1565"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="137"/>
            <end position="144"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00061030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8029129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1965" mass="221935" checksum="CF7218A72222121C" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H0N4</accession>
    <name>A0A9Q1H0N4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SRCR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_30614</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H0N4"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8028395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="99" mass="10830" checksum="9C74240E1A2DFAFA" modified="2023-09-13" version="1">MLCYGGPHYSTFLKVASVVCKELGYGPPRIADFSTYSFFVGKNFISFNDEWCRVDASEVLDCSLEGSFSTSVDICRGKVLKVECLPAEAEGKTKKVAKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H1X5</accession>
    <name>A0A9Q1H1X5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_26916</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H1X5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8030479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="116" mass="13162" checksum="1C951935AA6F1158" modified="2023-09-13" version="1">MTDAEQALEDALEEGLPEATPLRDFDHLRQNCKSKLRALVISTPLQQKLFLDAVFGVQEERSRIIDAKDGKDLKNLLYSLCEFFNEEEAKFLKLMRRGIEATLDLHSLATLVNAAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H6G5</accession>
    <name>A0A9Q1H6G5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">D-erythrulose reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_18144</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H6G5"/>
    <dbReference type="OrthoDB" id="1393670at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004090">
        <property type="term" value="F:carbonyl reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050038">
        <property type="term" value="F:L-xylulose reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005997">
        <property type="term" value="P:xylulose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000214">
        <property type="entry name" value="L-xylulose reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057326">
        <property type="entry name" value="KR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051737">
        <property type="entry name" value="L-xylulose/Carbonyl_redctase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44252">
        <property type="entry name" value="D-ERYTHRULOSE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44252:SF3">
        <property type="entry name" value="D-ERYTHRULOSE REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00822">
        <property type="entry name" value="PKS_KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0521">NADP</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ketoreductase" evidence="5">
        <location>
            <begin position="8"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8037347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="248" mass="26371" checksum="112BD959FB88F2D7" modified="2023-09-13" version="1">MNIDFGGKIALVTGASKGIGYDIALALAKGGAIKVYAVSRTLKDLEKLKDQNDKIEPVVCDLTDWDTTAKVIGDLGPVDLLVNNAGVSILQPFLDITPEVYSQIFDLNVKSVIQVSQIVAHKMIDKGSGGVIVNVSSMASTMSLKDHTCYCASKAALDSLTRSMAAELGSKNIRVNACNPTVTFTDMGRMAWGDPKRSAPVLSRIPIGRFAEVEDVTNVVLYLLSDKSAMLNGVTLPVDGGFTTVGFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H6S1</accession>
    <name>A0A9Q1H6S1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_21391</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H6S1"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8034515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="129" mass="14106" checksum="5ACBACA5167ED329" modified="2023-09-13" version="1">MCSRARLLIAATEFSGSYASFELRVSQERRARAEHSPAVPCPVKDMLRYAGNVPVRNSRDFPISLADLQLTQQTYVAGGRAACQGRHVQPRETSHCSDGIFGQLRQFRAPREPGKTCESGAFAGCTVSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H7I2</accession>
    <name>A0A9Q1H7I2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Double-strand break repair protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">HOLleu_22510</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">Nanhai2018</strain>
            <tissue evidence="21">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Core component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The MRN complex is involved in the repair of DNA double-strand breaks (DSBs) via homologous recombination (HR), an error-free mechanism which primarily occurs during S and G2 phases. The complex (1) mediates the end resection of damaged DNA, which generates proper single-stranded DNA, a key initial steps in HR, and is (2) required for the recruitment of other repair factors and efficient activation of ATM and ATR upon DNA damage. Within the MRN complex, MRE11 possesses both single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity. MRE11 first endonucleolytically cleaves the 5' strand at DNA DSB ends to prevent non-homologous end joining (NHEJ) and licence HR. It then generates a single-stranded DNA gap via 3' to 5' exonucleolytic degradation, which is required for single-strand invasion and recombination.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 16">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 16">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 16 18">Belongs to the MRE11/RAD32 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H7I2"/>
    <dbReference type="OrthoDB" id="30417at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030870">
        <property type="term" value="C:Mre11 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008296">
        <property type="term" value="F:3'-5'-DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000014">
        <property type="term" value="F:single-stranded DNA endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042138">
        <property type="term" value="P:meiotic DNA double-strand break formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097552">
        <property type="term" value="P:mitochondrial double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007095">
        <property type="term" value="P:mitotic G2 DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031573">
        <property type="term" value="P:mitotic intra-S DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00840">
        <property type="entry name" value="MPP_Mre11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000011">
        <property type="entry name" value="Double-strand break repair protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.110">
        <property type="entry name" value="Mre11, capping domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003701">
        <property type="entry name" value="Mre11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038487">
        <property type="entry name" value="Mre11_capping_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007281">
        <property type="entry name" value="Mre11_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041796">
        <property type="entry name" value="Mre11_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00583">
        <property type="entry name" value="mre11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10139">
        <property type="entry name" value="DOUBLE-STRAND BREAK REPAIR PROTEIN MRE11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10139:SF1">
        <property type="entry name" value="DOUBLE-STRAND BREAK REPAIR PROTEIN MRE11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04152">
        <property type="entry name" value="Mre11_DNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000882">
        <property type="entry name" value="DSB_repair_MRE11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01347">
        <property type="entry name" value="Mre11_DNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="9 16 18" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12 16 18" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8 16 18" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="11 16 18" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="10 16 18" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13 16 18" id="KW-0464">Manganese</keyword>
    <keyword evidence="15 16 18" id="KW-0469">Meiosis</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 16 18" id="KW-0540">Nuclease</keyword>
    <keyword evidence="14 16 18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mre11 DNA-binding" evidence="20">
        <location>
            <begin position="300"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="535"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="19">
        <location>
            <begin position="567"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="598"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="639"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="17">
        <location>
            <position position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR000882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR000882-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU003447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM01347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAJ8035325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="696" mass="79340" checksum="D80D08A3A4D3D500" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H841</accession>
    <name>A0A9Q1H841_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_19694</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8035881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H841"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8035881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="106" mass="12063" checksum="973A375BF6B78576" modified="2023-09-13" version="1">MLGSCSSKSHCIIDFSAGKAINFTNIILLEKVPEGCEDMDLPGTPFIFAIVDNPLAALAVWYSSFFMFNLQYQPKAPVLSKFVQRCFFEISLPSDWKQVYQGQRWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HAI5</accession>
    <name>A0A9Q1HAI5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_15600</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HAI5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8038236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="181" mass="20722" checksum="BB5D2B01B394CDCB" modified="2023-09-13" version="1">MVIDYRKYTTRVPTEVIIKESVVESVSTYKYLGIVTDDQIAWHDHIDYLVKKVSPRLYCLRKLNNFNINSVVCWGGNVDVTDKKRIDSVIKQAGRILGDDQFTIYSSYQHYVVAKLNTVLNGTSHPLYADPYSAIISGSGRMKLPYAVTKRQKLSFILQCIKPLATWFSNVYLSALSFRKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HAY1</accession>
    <name>A0A9Q1HAY1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CARD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_16433</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HAY1"/>
    <dbReference type="OrthoDB" id="1357022at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070513">
        <property type="term" value="F:death domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002020">
        <property type="term" value="F:protease binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01671">
        <property type="entry name" value="CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001315">
        <property type="entry name" value="CARD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037939">
        <property type="entry name" value="CRADD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15034">
        <property type="entry name" value="DEATH DOMAIN-CONTAINING PROTEIN CRADD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15034:SF5">
        <property type="entry name" value="DEATH DOMAIN-CONTAINING PROTEIN CRADD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00619">
        <property type="entry name" value="CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00114">
        <property type="entry name" value="CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50209">
        <property type="entry name" value="CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CARD" evidence="1">
        <location>
            <begin position="14"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8038878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="124" mass="14444" checksum="EEBCB177B753343C" modified="2023-09-13" version="1">MDKAGEVAIEIMAMSKEYRRVLDRSLPKLCEDLDVDPIIPSLIAEELFTDNDREELEAEHVRSKKVVKFVSILKRRSQRAFEVFVNCLKENEGSKHLAHHMEEVYRGITNQQPVSNQIHMQETH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HE34</accession>
    <name>A0A9Q1HE34_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Protein translocase subunit SecA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_12698</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10038397at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017038">
        <property type="term" value="P:protein import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1440.10">
        <property type="entry name" value="SecA, preprotein cross-linking domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000185">
        <property type="entry name" value="SecA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011115">
        <property type="entry name" value="SecA_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014018">
        <property type="entry name" value="SecA_motor_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044722">
        <property type="entry name" value="SecA_SF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036670">
        <property type="entry name" value="SecA_X-link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30612:SF0">
        <property type="entry name" value="CHLOROPLAST PROTEIN-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30612">
        <property type="entry name" value="SECA INNER MEMBRANE COMPONENT OF SEC PROTEIN SECRETION SYSTEM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21090">
        <property type="entry name" value="P-loop_SecA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07517">
        <property type="entry name" value="SecA_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00957">
        <property type="entry name" value="SecA_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81767">
        <property type="entry name" value="Pre-protein crosslinking domain of SecA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51196">
        <property type="entry name" value="SECA_MOTOR_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-1278">Translocase</keyword>
    <keyword evidence="7" id="KW-0811">Translocation</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SecA family profile" evidence="12">
        <location>
            <begin position="568"/>
            <end position="1328"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="688"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="1164"/>
            <end position="1344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="63"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="63"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8041791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="2356" mass="266995" checksum="AB1249215D6DCE30" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HES6</accession>
    <name>A0A9Q1HES6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_13526</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8042463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HES6"/>
    <dbReference type="OrthoDB" id="6143625at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="779"/>
            <end position="798"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8042463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="798" mass="91083" checksum="974AE9F5ABC1FB9F" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HG21</accession>
    <name>A0A9Q1HG21_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cone-rod homeobox protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_07363</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HG21"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051306">
        <property type="entry name" value="Homeobox_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46123">
        <property type="entry name" value="MIX-TYPE HOMEOBOX GENE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46123:SF4">
        <property type="entry name" value="MIX-TYPE HOMEOBOX GENE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 5 6" id="KW-0371">Homeobox</keyword>
    <keyword evidence="4 5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="7">
        <location>
            <begin position="56"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="5">
        <location>
            <begin position="58"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8044580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="106" mass="11655" checksum="B1AA88284A09843E" modified="2023-09-13" version="1">MYVKHNTFIASQLAGDGIIHTAPSSSTGFSSRQDVVSGTGGDLLHCNELPYLKTKKAGRRQRTSFSMEQLEKLEHAFKCSPYPGINEREDLSAQIKIAESRIQVSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HGF9</accession>
    <name>A0A9Q1HGF9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_11405</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8044053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HGF9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8044053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="62" mass="6738" checksum="4DA3AB82875FEE2E" modified="2023-09-13" version="1">MDLANGRRTPYRKPNDAPIKINVLSNHPQPVISLIPGAISTRLSALSSDQDAFNSSATEYVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HJJ9</accession>
    <name>A0A9Q1HJJ9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_06426</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HJJ9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8047431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="122" mass="14116" checksum="8F089F00B44CFFC3" modified="2023-09-13" version="1">MHLKEPLTNTELEFPSNHGRLCDNYLFRLRKKPIKGTRVFCIPCQGHTTTGQCQDTYVRETKRSLKSRFLENLCPNLISSEVSQYIHIESPGHHINLDEVKIIERERCQEGNLYSGQSAITQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HK46</accession>
    <name>A0A9Q1HK46_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_00247</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HK46"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8048076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="52" mass="5683" checksum="804EDA7EB7742A0E" modified="2023-09-13" version="1">MSFLSTHSRSSARSQGPSAKGYPDSRQTRTLSSLPPGNTTTHHEKVDKPTTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HLN8</accession>
    <name>A0A9Q1HLN8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transposase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_02942</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HLN8"/>
    <dbReference type="OrthoDB" id="6629909at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33053">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8049963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="541" mass="60413" checksum="F5D8A488B14A0C4C" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0Y8V5</accession>
    <name>A0A9Q0Y8V5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_44459</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000905">
        <property type="protein sequence ID" value="KAJ8017873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0Y8V5"/>
    <dbReference type="OrthoDB" id="6151961at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37162:SF10">
        <property type="entry name" value="DUF4371 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37162">
        <property type="entry name" value="HAT FAMILY DIMERISATION DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8017873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="212" mass="24384" checksum="4D56AFD2441FF67D" modified="2023-09-13" version="1">MGTKKRTKTVDQKYKDAYIAEWPVIRKSQKGDTYAFCTSCTMDFSVSHGGRNDCLKHVSTDRHRRNSASITSNSKILDFVTRESQFEVINAEVFFSNFIVEHNLPIGICDHVGPLFKKMFTDSKIAQQYGCARTKTSAILNTLGDHACRWGMTRQSVPTRCGATFVERGDMSLVHAPHRTLPMPVRMWSRHLNPRKQAQNIPLRNWRRQHKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0Y9U6</accession>
    <name>A0A9Q0Y9U6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Kinesin-like protein KIN-4A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_43059</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000197">
        <property type="protein sequence ID" value="KAJ8018772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0Y9U6"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005875">
        <property type="term" value="C:microtubule associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051231">
        <property type="term" value="P:spindle elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969">
        <property type="entry name" value="CHROMOSOME-ASSOCIATED KINESIN KIF4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969:SF15">
        <property type="entry name" value="CHROMOSOME-ASSOCIATED KINESIN KIF4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="8">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8018772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="95" mass="10796" checksum="FF144DD94D8F01D4" modified="2023-09-13" version="1">MVSEEPEVMALLKKGSDGRATGSTGMNETSSRSHAIFTINIEQISRDDRLVNFISIEIEEVELGATFLLYSFLPKICYERQVLYIHIKGSWSRQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-10-08" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YBK8</accession>
    <name>A0A9Q0YBK8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">DNA topoisomerase 2</fullName>
            <ecNumber evidence="16">5.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">HOLleu_41238</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">Nanhai2018</strain>
            <tissue evidence="20">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 22">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="16">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 16">Belongs to the type II topoisomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="16"/>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8019588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="276498at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003918">
        <property type="term" value="F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000712">
        <property type="term" value="P:resolution of meiotic recombination intermediates"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000819">
        <property type="term" value="P:sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16930">
        <property type="entry name" value="HATPase_TopII-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00187">
        <property type="entry name" value="TOP4c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03481">
        <property type="entry name" value="TopoIIA_Trans_ScTopoIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03365">
        <property type="entry name" value="TOPRIM_TopoIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.268.10:FF:000002">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.40:FF:000003">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.30:FF:000001">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000008">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000004">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.670:FF:000001">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.199.10:FF:000002">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.670">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.199.10">
        <property type="entry name" value="Topoisomerase II, domain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.268.10">
        <property type="entry name" value="Topoisomerase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050634">
        <property type="entry name" value="DNA_Topoisomerase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001241">
        <property type="entry name" value="Topo_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013760">
        <property type="entry name" value="Topo_IIA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013758">
        <property type="entry name" value="Topo_IIA_A/C_ab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013757">
        <property type="entry name" value="Topo_IIA_A_a_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013759">
        <property type="entry name" value="Topo_IIA_B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013506">
        <property type="entry name" value="Topo_IIA_bsu_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002205">
        <property type="entry name" value="Topo_IIA_dom_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001154">
        <property type="entry name" value="TopoII_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018522">
        <property type="entry name" value="TopoIIA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031660">
        <property type="entry name" value="TOPRIM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034157">
        <property type="entry name" value="TOPRIM_TopoII"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10169:SF38">
        <property type="entry name" value="DNA TOPOISOMERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10169">
        <property type="entry name" value="DNA TOPOISOMERASE/GYRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00204">
        <property type="entry name" value="DNA_gyraseB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00521">
        <property type="entry name" value="DNA_topoisoIV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01751">
        <property type="entry name" value="Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16898">
        <property type="entry name" value="TOPRIM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01158">
        <property type="entry name" value="TOPISMRASEII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00418">
        <property type="entry name" value="TPI2FAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00433">
        <property type="entry name" value="TOP2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00434">
        <property type="entry name" value="TOP4c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56719">
        <property type="entry name" value="Type II DNA topoisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52040">
        <property type="entry name" value="TOPO_IIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00177">
        <property type="entry name" value="TOPOISOMERASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12 15 16" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="13 15 16" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 15 16" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="Toprim" evidence="18">
        <location>
            <begin position="479"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="domain" description="Topo IIA-type catalytic" evidence="19">
        <location>
            <begin position="739"/>
            <end position="1196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="25"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1122"/>
            <end position="1143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1223"/>
            <end position="1574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="1125"/>
            <end position="1137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="1228"/>
            <end position="1240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1281"/>
            <end position="1294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1311"/>
            <end position="1322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="1327"/>
            <end position="1342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="1347"/>
            <end position="1362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="1486"/>
            <end position="1498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="1510"/>
            <end position="1527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="1531"/>
            <end position="1545"/>
        </location>
    </feature>
    <feature type="active site" description="O-(5'-phospho-DNA)-tyrosine intermediate" evidence="15">
        <location>
            <position position="829"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU362094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS52040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAJ8019588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1574" mass="177601" checksum="F28C40DBEBD5C4FC" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YCE9</accession>
    <name>A0A9Q0YCE9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_41350</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8019675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YCE9"/>
    <dbReference type="OrthoDB" id="6361178at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8019675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="196" mass="21706" checksum="F4896F7FFC5F6EF9" modified="2023-09-13" version="1">MRYGKRKNPIIFGGGQRSSGVTGGQTLKTSLTRYLKMPHIERKNPIVFGGGQRSPGVTRGQNLKTLLTQYLEMRYGKRKNPIIFGGGQRSSGVTGGQTLKTSLTRYLKMRHGKRKNPIIFGGGQRSFGVTGGQSLKTLLTRYLKMRYGKRKNPFTFGGGQRSSGVTGGQTLKSLLTRYLKKGSSDSSQTWYMDPPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YDW8</accession>
    <name>A0A9Q0YDW8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_43913</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000526">
        <property type="protein sequence ID" value="KAJ8018222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YDW8"/>
    <dbReference type="OrthoDB" id="10260017at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8018222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="108" mass="12514" checksum="ECB3066C58D6C5F9" modified="2023-09-13" version="1">MHTVLYFFQNPDQDDIPYPLTRKEAFHFLENVLLISDPAKLLKKDSVMFLNTFIHGMTTKIPFTSIPDVSKPINDKHLPTFAECKEAIFSREGGDCFYKNIFLKLCLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YDX2</accession>
    <name>A0A9Q0YDX2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Tetratricopeptide repeat protein 12</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_40644</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YDX2"/>
    <dbReference type="OrthoDB" id="629492at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070286">
        <property type="term" value="P:axonemal dynein complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007288">
        <property type="term" value="P:sperm axoneme assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043195">
        <property type="entry name" value="TTC12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46540">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46540:SF1">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="157"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8020923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="689" mass="76505" checksum="B9605285DBD84CE2" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YEF0</accession>
    <name>A0A9Q0YEF0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_42917</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000162">
        <property type="protein sequence ID" value="KAJ8018869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YEF0"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8018869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="195" mass="22762" checksum="B7934147E1CB5047" modified="2023-09-13" version="1">MDIKHLHNMFVYCNIVEPHAVGHAKVPVIRVVTVKRRYGEDIISIYTNIYYHPVEQKYFENIEIDIRDSVRFKMSFLRGIAIVTLHFPLQKRRSLFKKFLSCIITVIFTSIRAITKTLTFIKSVTAARLWRRKRTARTLYREYTGWIVSHGRSPAEERSQSIGKRSLAHRHECCGECLGRKILQGLCKNQGSSCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YHG0</accession>
    <name>A0A9Q0YHG0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_40208</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YHG0"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="166"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="200"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="265"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="332"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="402"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8020579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="423" mass="49974" checksum="3A9A1E9C377514C6" modified="2023-09-13" version="1">MDFQEVTLLINNSREKFKTRVPIMLMVVMWSLGIIDWSPPRCDGNQYYLYCPFIGRSRLHRKVTFVVSVGWVFTTAPTAYFGAITYFVCFFGLKEHFLRFVIVVVCYQPSLISGTIIFISAFFINRCKKSDIDEFNMSWINLFQESVFLQRINFIKKFNMFILPRLAVYVFFNICSLAVSFVILLLYIEVYNDCLTWVSYIWTASEFIGLIYLAQFCYFLYLYRVSLEEQFKETERFTKGNAGKLEDCTKKVKNLFCSYFQLRKLFLLWLSIIFFSITFGIAAYATWSYKKPEVQTNSTVLPTQYPIHPKGIKKYCPYSCDDPKYFENQHHLLIYNVLAFAKLLIPAVLSFSVMRGLDIKYMWNRFILRLRLISTTDHLDFWNTLIRYTKDLHPENQLDTKLRFIIPVLGLAFGFLGGWKSHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YJZ9</accession>
    <name>A0A9Q0YJZ9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_36439</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YJZ9"/>
    <dbReference type="OrthoDB" id="10546185at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8023872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="156" mass="17207" checksum="3E4B7736500AE5EA" modified="2023-09-13" version="1">MELQKQNEVVITQQPQSVPSYVVIQTAPRNNVPAIGALVFSLVTLVCCPGSLFCTIPSCICAIVALKGDGSDSEKTMYITSFITTAAFWILAVISAIILVILAVARVFAAASASINDHFDYIYDYFNNNNPFENVDSNINNYDPFDYFDNIFNNNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YLM3</accession>
    <name>A0A9Q0YLM3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ferric-chelate reductase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_40901</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8021121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YLM3"/>
    <dbReference type="OrthoDB" id="6418377at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="CDD" id="cd08544">
        <property type="entry name" value="Reeler"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.4060">
        <property type="entry name" value="Reeler domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003410">
        <property type="entry name" value="HYR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002861">
        <property type="entry name" value="Reeler_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042307">
        <property type="entry name" value="Reeler_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24273">
        <property type="entry name" value="FI04643P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24273:SF32">
        <property type="entry name" value="HYALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02494">
        <property type="entry name" value="HYR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02014">
        <property type="entry name" value="Reeler"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50825">
        <property type="entry name" value="HYR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51019">
        <property type="entry name" value="REELIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040488128" evidence="2">
        <location>
            <begin position="24"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="Reelin" evidence="4">
        <location>
            <begin position="14"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="HYR" evidence="3">
        <location>
            <begin position="176"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="HYR" evidence="3">
        <location>
            <begin position="261"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="HYR" evidence="3">
        <location>
            <begin position="348"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8021121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="473" mass="49747" checksum="FB59D8F288C986DC" modified="2023-09-13" version="1">MAQLKVLLSTFFLVLTAVTLVECFPGGAGTQACVTLVPLHGTQPQPAPVPYQITLSQSTFAPGDQILVTVDATPGNTNVYRGLALQARPAVGSTDTTGTFTILPNDQSNFRFYDCADGSPTGGVTHTNRNDKSLPQRFLWTPSDGFTGDVQFRLTVVREFSIFWTDIPSAVLRLQQGNNLPVVTCPGPVSVQSLPNNVGNRATFLPTCTDLDQSDASLDLTCSPASNSFFPLGTTPVVCICTDNQGETDTCTIPVTINPVLENTPPAITCPGSVSVRSFQQIEGNFVSWDAPICTDVDQPGINLASTCEPASGSFFQGLGTTVVTCTCSDNQRATDTCTISVTITSFTDVNTLPVVTCPGPVSVQSLPNNVGNRATFLPTCTDLDQSDASLDLTCSPASNSFFQLGTTPVVCTCTDNQGATDTCTISVTISPVGQGGVCDGNPCFPNECRVSLLSGPDRYTCHSTTGAPCPER</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YM72</accession>
    <name>A0A9Q0YM72_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Integrin beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">HOLleu_35095</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="22">Nanhai2018</strain>
            <tissue evidence="22">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 18">Cell membrane</location>
            <topology evidence="1 18">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 18">Belongs to the integrin beta chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8025013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YM72"/>
    <dbReference type="OrthoDB" id="410592at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008305">
        <property type="term" value="C:integrin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033627">
        <property type="term" value="P:cell adhesion mediated by integrin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007229">
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    <sequence length="800" mass="88028" checksum="D804F3934B647FB3" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YML1</accession>
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        </submittedName>
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        <name type="common">Black long sea cucumber</name>
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        <dbReference type="NCBI Taxonomy" id="206669"/>
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                <person name="Chen T."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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            <authorList>
                <person name="Chen T."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        </source>
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        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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    </dbReference>
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        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </feature>
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    </feature>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8025237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="394" mass="43025" checksum="8624A02C86660E7E" modified="2023-09-13" version="1">MEDFLFLRRPKDLKEVVGLAAEFEANCTARLQRQATAVKDQRGVRSITEKELSKPNFRKTKPGKRQWTEPVGRGEVQGSYIERPAEQVSRELADKRDMGDRDQICVTPSGPTALTEAERKGRNSDVPVVARKHESGAGSPVDSGQGGRDPYGVAPSKPVDAGSPTGGNNMAGCSMVAEEKLEQVDDCCNKSLHSKSNVSGKRAYGKLPQTTPQDTQHETMGRSCKESPGEIGGRPRVGTVRKGRLVSVYLEGAIQGETIKFLVDSGSSMSFLSKKKYLELVPDKDEDLKPTGVPQQLADGSPLEVLGQVELVVKLEGRPLIVEFQVAELDLEGILGLDFLHSHRCQWDWDNCSMLVAGVAVPLIRGMKYDDPIVSKVQAKDNFVVPAGHEMLVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YNA9</accession>
    <name>A0A9Q0YNA9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_33245</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YNA9"/>
    <dbReference type="OrthoDB" id="10068277at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="200"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="200"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8025633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="255" mass="29300" checksum="EC2CFD7D738E4CEF" modified="2023-09-13" version="1">MEHTKKLVLVSPEVLERVKGGRSQTLEKTKVQELEEQLSSVLSDPRLDDATKMKRYSEVLQRYTVYHAKANEPLKVRVLPETSSSVQPGDNLQKRVIEQDVIESVPRAYKSKATQLLGKIASNDSGLDWTDRGELVVNDHTIKGSHIVDLVNDMMRKRKSATPIGMDEFTKALARINTPRELVDNPDRWQDLSRWRDLGDAPRKVRERPLSEEVPEDNDGDHQTCYAKANHLPVKKRPDLSPRKSRKIMRAWEKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YQM0</accession>
    <name>A0A9Q0YQM0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C17orf113 probable zinc finger domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_35948</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YQM0"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057456">
        <property type="entry name" value="Znf_C17orf113"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25431">
        <property type="entry name" value="zf-C17orf113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C17orf113 probable zinc finger" evidence="2">
        <location>
            <begin position="113"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8023491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="159" mass="18004" checksum="B57A44F4A99A66F1" modified="2023-09-13" version="1">MSKRRKLDPATPPVGTLDRFFRKQTPSDRENYPTDGPANVPQPGPSGRDGDTVTEDDAHHQPDAPSVEEPGADFPAQAPSTSGAKKKTKGKQANYFHPEWKKKRPWLHHEPDIGMFCLLCQKYKKMPYGRRKWNTAPCVRMREGSVKEHEESESHKDAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YRC9</accession>
    <name>A0A9Q0YRC9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">PH-interacting protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_32149</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YRC9"/>
    <dbReference type="OrthoDB" id="10265743at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052060">
        <property type="entry name" value="Bromo_WD_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057451">
        <property type="entry name" value="BRWD/PHIP_AD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16266:SF17">
        <property type="entry name" value="BRWD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16266">
        <property type="entry name" value="WD REPEAT DOMAIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25313">
        <property type="entry name" value="BRWD_AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bromo" evidence="4">
        <location>
            <begin position="392"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="4">
        <location>
            <begin position="610"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="108"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="500"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="711"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="500"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="522"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="556"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="728"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="790"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="805"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="819"/>
            <end position="831"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8027103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="864" mass="99727" checksum="CF0B102365E89D08" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YSJ9</accession>
    <name>A0A9Q0YSJ9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">C17orf113 probable zinc finger domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_33431</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YSJ9"/>
    <dbReference type="OrthoDB" id="10060990at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057456">
        <property type="entry name" value="Znf_C17orf113"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46880:SF5">
        <property type="entry name" value="DUF4371 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46880">
        <property type="entry name" value="RAS-ASSOCIATING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25431">
        <property type="entry name" value="zf-C17orf113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C17orf113 probable zinc finger" evidence="1">
        <location>
            <begin position="55"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF25431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8025782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="375" mass="41809" checksum="0C1F4619E62E66F1" modified="2023-09-13" version="1">MAKQISLLNFGFTNAAAQNKAPPKGKAATKREYDVNKRSRGFVASWVDEFPGILHDNDAGVIFCSTCQNHPNIADQQSVFFTGGCGTYRKQSLQAHWLSPRHIMCVEAKVAKKRAQGNDLVGPIDNVIKRMSEKNRNILKKVFNTAYMILKCELPFTLFPNMLKLQIKNGSDIDGLSSYRTNTACKRFTQYIVGDIREPLKDAINNARVFSVIYDGATDASVTEVEIVYCRYVRDGKPLNCFVNLHEVEHAHAEGVFSAIDNAMVSAGIENWKEKLVGAGSDGAKVNVGINNSVATRMQGDGREHVVIVHCVAHRLELAILGAIKENEMLQTVQDMLNKIHKHYHYSPKALRELEMVAEAMGEKFLKPQRLSGTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YTY9</accession>
    <name>A0A9Q0YTY9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">SMC5-SMC6 complex localization factor protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_33905</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM178 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8026146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6158547at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044276">
        <property type="entry name" value="CANIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026161">
        <property type="entry name" value="FAM178"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16046">
        <property type="entry name" value="SMC5-SMC6 COMPLEX LOCALIZATION FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16046:SF9">
        <property type="entry name" value="SMC5-SMC6 COMPLEX LOCALIZATION FACTOR PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14816">
        <property type="entry name" value="CANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Coiled-coil SMC6 And NSE5 INteracting (CANIN)" evidence="3">
        <location>
            <begin position="1114"/>
            <end position="1482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="56"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="201"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="468"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="571"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="630"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="796"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="979"/>
            <end position="1053"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1066"/>
            <end position="1085"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1616"/>
            <end position="1754"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1781"/>
            <end position="1938"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1983"/>
            <end position="2016"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="10"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="56"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="104"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="209"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="239"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="298"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="345"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="484"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="514"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="630"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="667"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="686"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="818"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="873"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="895"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="942"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="988"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1021"/>
            <end position="1031"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1627"/>
            <end position="1636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1644"/>
            <end position="1666"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1667"/>
            <end position="1707"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1781"/>
            <end position="1796"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1827"/>
            <end position="1846"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1858"/>
            <end position="1884"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1886"/>
            <end position="1900"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1909"/>
            <end position="1921"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8026146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="2138" mass="235669" checksum="C18515262634EF6F" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1B8J6</accession>
    <name>A0A9Q1B8J6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_44798</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010001252">
        <property type="protein sequence ID" value="KAJ8017646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1B8J6"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8017646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="65" mass="7624" checksum="543FCEB257B67245" modified="2023-09-13" version="1">MLVVNWTFLQFKPWKFSVIYVTKSPILVGSYLLRSSLRFSPVTDNCLQDLRKVEEAQLNVCFQPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BA10</accession>
    <name>A0A9Q1BA10_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA-directed DNA polymerase</fullName>
            <ecNumber evidence="2">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_42671</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="2"/>
    <dbReference type="EMBL" id="JAIZAY010000111">
        <property type="protein sequence ID" value="KAJ8019010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.690">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1600.10">
        <property type="entry name" value="Palm domain of DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.960.10">
        <property type="entry name" value="VSR Endonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004868">
        <property type="entry name" value="DNA-dir_DNA_pol_B_mt/vir"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023211">
        <property type="entry name" value="DNA_pol_palm_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33568">
        <property type="entry name" value="DNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33568:SF3">
        <property type="entry name" value="DNA-DIRECTED DNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03175">
        <property type="entry name" value="DNA_pol_B_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0235">DNA replication</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA-directed DNA polymerase family B mitochondria/virus" evidence="10">
        <location>
            <begin position="675"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed DNA polymerase family B mitochondria/virus" evidence="10">
        <location>
            <begin position="1032"/>
            <end position="1204"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed DNA polymerase family B mitochondria/virus" evidence="10">
        <location>
            <begin position="1235"/>
            <end position="1326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="107"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="170"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8019010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1422" mass="163988" checksum="2B1521FAF553BC72" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BC97</accession>
    <name>A0A9Q1BC97_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Zinc finger protein 20</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_42226</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000043">
        <property type="protein sequence ID" value="KAJ8019277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BC97"/>
    <dbReference type="OrthoDB" id="9439903at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031519">
        <property type="term" value="C:PcG protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002737">
        <property type="entry name" value="AGAP008430-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000744">
        <property type="entry name" value="zinc finger E-box-binding homeobox 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR PROTEIN YY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003:SF26">
        <property type="entry name" value="ZINC FINGER PROTEIN 367"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="287"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="315"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="343"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="19"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="19"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ8019277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="420" mass="47263" checksum="014B87716254A77F" modified="2023-09-13" version="1">MNVRKCDMEHHYGYPTSVSQFHSQGGMASSEHSQHKTRNQQSVTTGRDCETLEPLVLYSQNKAAINRAFVVYERCLDILFHCISCKRCDGPTQNIAKFVDSDSLFVKLYCSSGHQLLHWSSRSSEGIHIQRSSVDLARVERVNSVKGNDKSRAFKDDTSGDLSCDERRNVNNCAEPNRGYNGSQSALNEPILVKIEEDEEFPSSCSESDKQGVQTSPQVAHIVHSCDPFAKGSVNSDQTQALRHDLHTIDSENHVLHEELAPGLSKQIEDDNGQQPITFIERIRTPTTCRFCCKVFPCASDLESHERTHTGERPYECRYCSKGFIHWSHVKSHEFTHTGFRAFACCFCGKRFPLSTQCRAHERTHVIEPQPVDTSIIPSSSDSGGLVETNTREETEAQNVENATDTGTVCVRYNFHPFLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BDH2</accession>
    <name>A0A9Q1BDH2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">TNFR-Cys domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_38329</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BDH2"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.10">
        <property type="entry name" value="Tumor Necrosis Factor Receptor, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001368">
        <property type="entry name" value="TNFR/NGFR_Cys_rich_reg"/>
    </dbReference>
    <dbReference type="SMART" id="SM00208">
        <property type="entry name" value="TNFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50050">
        <property type="entry name" value="TNFR_NGFR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040153494" description="TNFR-Cys domain-containing protein" evidence="2">
        <location>
            <begin position="20"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="repeat" description="TNFR-Cys" evidence="1">
        <location>
            <begin position="79"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="TNFR-Cys" evidence="3">
        <location>
            <begin position="79"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8021199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="148" mass="16377" checksum="88904E8B97ED6FC6" modified="2023-09-13" version="1">MDLLPVGFMLGSVIGGCVAQSENYCPSPPVPTNTSKQNLPYVEREMENEWCFYDPSEAAGGLPCRVGYHCNLIGPSCLPCPHDEIKTYQPKQNNCPKCILCTDCEKEGMEEVSRCTRGNNAVCQKRSPQSTGEIINLLHCSYLTCCLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BG73</accession>
    <name>A0A9Q1BG73_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">F-box/WD repeat-containing protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_32892</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BG73"/>
    <dbReference type="OrthoDB" id="435188at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052301">
        <property type="entry name" value="SCF_F-box/WD-repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14381">
        <property type="entry name" value="DACTYLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14381:SF1">
        <property type="entry name" value="F-BOX_WD REPEAT-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="145"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8025355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="408" mass="46869" checksum="C94643D71D2F8D01" modified="2023-09-13" version="1">MLLNNLPAELLSKILGFLDKEDLVNICQVCKQLRKICLDDLVWRSLGEPLANFRPNHFRQLRKLFHGRQESISSKNSLKEACRIAHNWQSHKPYDCTVQKFRQKLLPWLCFSGNALFFSHLNSIISSRTGSRFRNGIDFKHRRYYRGHKSDVVRFAVTDSQIISGGCDRQILVWNKDSCDLYQQIPLEGIVTSLDACNSTIVTGIRERGISVFRKNEHGFVSSYHYPMKDIVWTLAVDTNNRYFTAGSSCCLDKQPLKVFDVERGDIVNVCGKDYPYGAGVLALHYQDPRTFLSCGYDCSLNCWDLRGSCRVPSISFPDEDQSFYYSLDSDHDNFIACGLCRFGQIKFWDKRHPSRNLQSFFVGNVGRLDNSSPVYSLKVTATHLYAALSQSLQTLDFTHNLCYRPDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BHJ8</accession>
    <name>A0A9Q1BHJ8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA-directed DNA polymerase</fullName>
            <ecNumber evidence="2">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_35110</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="2"/>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8025022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BHJ8"/>
    <dbReference type="OrthoDB" id="6751099at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004868">
        <property type="entry name" value="DNA-dir_DNA_pol_B_mt/vir"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33568">
        <property type="entry name" value="DNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33568:SF3">
        <property type="entry name" value="DNA-DIRECTED DNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03175">
        <property type="entry name" value="DNA_pol_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0235">DNA replication</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA-directed DNA polymerase family B mitochondria/virus" evidence="9">
        <location>
            <begin position="136"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8025022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="385" mass="44774" checksum="B7BB17B85552DB26" modified="2023-09-13" version="1">MFYRVVKNKDGTVRPHNCNEYFCRICKINVKDGHNCFIQVYEDKKKRKEKGQDEEGGEGRIRPQETPHKYIFFDFETIQETGIHVPNMCVVQMVCDLCITEPFESECLVCEEKQVIFQGPNTKREFSEWLLAPPRNNSTCIAHNLKGFDGYFILQHLYDNGVVPEIITNGAKVMFIKLLGNSIRFIDSVNFLPMPLSNMPKTFGFNELKKGYFPRLFNTTENQTYIGHSPEASYYAPDVMSSEKRKDFFMWYETEKNKGLLFDFQKELVAYCISDVDILRRCCLKFRSLFMDITCKEIDDNVEDEAEEGDGVVTEMCGVDPFKHCITIASACNLVFRRNYMKPNSIAVFINDKPKSYSFAALEWLYYESKQRGVYIQPGSCYGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BHX3</accession>
    <name>A0A9Q1BHX3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">General transcription factor II-I repeat domain-containing protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_31733</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BHX3"/>
    <dbReference type="OrthoDB" id="6431883at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040647">
        <property type="entry name" value="SPIN-DOC_Znf-C2H2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45913">
        <property type="entry name" value="EPM2A-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45913:SF5">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR II-I REPEAT DOMAIN-CONTAINING PROTEIN 2A-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18658">
        <property type="entry name" value="zf-C2H2_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SPIN-DOC-like zinc-finger" evidence="1">
        <location>
            <begin position="23"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8026793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="120" mass="14327" checksum="DE347E5214BB4E79" modified="2023-09-13" version="1">MAAPKSNSKKRKVTEEGRVFNPEWTRDYFFIEEQGKPVCLICKRGISVMKLNNITRHYNTSHRAEYRQLHGDARKQRIDKLMKTMKQQQECFFWPPAKDKPLLAKPYCLTKNCREMQTLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BI31</accession>
    <name>A0A9Q1BI31_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_33495</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BI31"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040231907" evidence="1">
        <location>
            <begin position="26"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8025832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="310" mass="34731" checksum="9E7A37422DB0FA34" modified="2023-09-13" version="1">MSSGWIRLLLLASCLACLNVRGIEGKTLYDLRKFHFQGKGARFAVSKEELERKLSGIKKQDGVCGNDVNLELLHTTELLQEAEIFIAYIVMEDLTLSEELSCEMKEKVAVNSSHVFISVPFVSGERHGLLLVAHFTDSRKQTFFPYSTNYDRVSRIDSIDETDSGTVTLRKGNDVMIMNIQSNVTCHRDEVKELREDVDEVIGKLVSSTDYPLPFDWGCDILGESVSFCETLRKNQCGLGRAGSDVCIEMTSRNSKRCPASMQILSMTGLFQDAVPYLSTPASSFEGVWVDREHGTNIVFPCFTSVTKYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BIE6</accession>
    <name>A0A9Q1BIE6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_32050</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BIE6"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008028">
        <property type="term" value="F:monocarboxylic acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF303">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="138"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="167"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="199"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="227"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="282"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="305"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8027034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="332" mass="36411" checksum="450BB9783FB33391" modified="2023-09-13" version="1">MKETSSDDIFGITVSLTDQFSFVGMMILPIVLDKFQDFYGLQGGIFLFGGLNLNIMVAGVVMKGHIGRKKIHQPQHDHTESGSGFVVLLHQHWLVLVINPGIYLLCITALLHFFNVIAWALFLVSLGEESGLSQSNAVLLASCGGFGALFGRVWSIILFSLKVESPFQFFGIPALLQFVSLLGIVFIKDNFFLSMMMSFVAGMSFGEIGAGCSGLCALKTSKSDFRLALVMMFISLAVGNELGGGVSGKCIKEKYLKFCESLKCFESNLSIKTLNCNLRENVLYSYLLCLTAGCVYDIFGSSRSVFILMATVSLLITLVSALWNIWDNQRKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BJY1</accession>
    <name>A0A9Q1BJY1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">guanylate cyclase</fullName>
            <ecNumber evidence="2">4.6.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_30136</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.2" evidence="2"/>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BJY1"/>
    <dbReference type="OrthoDB" id="6127067at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008074">
        <property type="term" value="C:guanylate cyclase complex, soluble"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004383">
        <property type="term" value="F:guanylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019934">
        <property type="term" value="P:cGMP-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070482">
        <property type="term" value="P:response to oxygen levels"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.260:FF:000002">
        <property type="entry name" value="guanylate cyclase soluble subunit alpha-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1230:FF:000007">
        <property type="entry name" value="Guanylate cyclase soluble subunit alpha-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1520.10">
        <property type="entry name" value="H-NOX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.260">
        <property type="entry name" value="Haem NO binding associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018297">
        <property type="entry name" value="A/G_cyclase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038158">
        <property type="entry name" value="H-NOX_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011644">
        <property type="entry name" value="Heme_NO-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011645">
        <property type="entry name" value="HNOB_dom_associated"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042463">
        <property type="entry name" value="HNOB_dom_associated_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024096">
        <property type="entry name" value="NO_sig/Golgi_transp_ligand-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45655">
        <property type="entry name" value="GUANYLATE CYCLASE SOLUBLE SUBUNIT BETA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45655:SF6">
        <property type="entry name" value="HEAD-SPECIFIC GUANYLATE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07700">
        <property type="entry name" value="HNOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07701">
        <property type="entry name" value="HNOBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111126">
        <property type="entry name" value="Ligand-binding domain in the NO signalling and Golgi transport"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00452">
        <property type="entry name" value="GUANYLATE_CYCLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0141">cGMP biosynthesis</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Guanylate cyclase" evidence="11">
        <location>
            <begin position="539"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="473"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="22"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="43"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="58"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="68"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8028015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="743" mass="82368" checksum="55D1F239E245BE0C" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BK82</accession>
    <name>A0A9Q1BK82_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Zinc finger CCHC domain-containing protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_30266</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BK82"/>
    <dbReference type="OrthoDB" id="6775559at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002218">
        <property type="term" value="P:activation of innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042509">
        <property type="entry name" value="ZCCHC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22639">
        <property type="entry name" value="GAG-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22639:SF3">
        <property type="entry name" value="ZINC FINGER CCHC DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="56"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="214"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8028119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="356" mass="39577" checksum="B5CC1C87EB22F55B" modified="2023-09-13" version="1">MVRLSVAVFCPCLIIPPSIKELDNIKLTLRTIYCTVISPTWWQKLLGTILGQPRPCMKCGVIGHEAKECNLPKCFKCQGLGHTSKTRVEDDVCTTCVKPGHTFRNCPVSFSCKFKQSTAWVVGEASPVKSDGDASPRPPMIHAHENSMQPLVIPDTQDPSVMDTVSEVNSQEDLFEGKAAAELLASAPKNWAEATEEIQMKSVKVVKEKEKVKLKEHESLVPEKAQSQPSHLKGAVTDAPSRISSSQPDVGQDPSKLSKRNSVSPPTPNKHVGHTRSRSNFTEDPKDIVKMTQIFIGEEPWHSCYSKDCKVFPKYTLLKEHVQIFHPDLKPRSYECLLKSCKADYTTHKIGFGTLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BKC5</accession>
    <name>A0A9Q1BKC5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Carnosine N-methyltransferase 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_30298</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BKC5"/>
    <dbReference type="OrthoDB" id="5984880at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8028146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="120" mass="13818" checksum="F68B0A1332614EB6" modified="2023-09-13" version="1">MLKQLKKKFPLIECIVVEPGIEHIARYKELVDEQKDALKGVTFDWRQETIQEFCKANADASKKFHFVSAIHSCYFIARKDLDFYLKAIYGWTKGKILIMIGPGNEGFCVCTVRGDTRLVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BPC2</accession>
    <name>A0A9Q1BPC2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_26747</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BPC2"/>
    <dbReference type="OrthoDB" id="8946688at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8030352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="133" mass="14844" checksum="5BA053FAA62388FB" modified="2023-09-13" version="1">MAHFDAFIAANRSKPVKPNLRPQERKAIGSLRRNPDITIRPADKGGALTILNTADYIKEVESQLSNQVFYRTLTQNPVKLLTHQVDLLCRNISASSTAKVREFIPIQPKLGTFYTLPKIHKLPTLIASSLPTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BPS5</accession>
    <name>A0A9Q1BPS5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_27003</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BPS5"/>
    <dbReference type="OrthoDB" id="10601332at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040444361" evidence="3">
        <location>
            <begin position="26"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="305"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="432"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="439"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8030550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="491" mass="53645" checksum="07E98F42F49B806A" modified="2023-09-13" version="1">MALMCLVIKLCSVTVMLSIISLTGSQEILFELKDRQYQEILFSGDVTVAMVCKIITVNTAEVKIINSGGELVTTNNGKGNCLEFVISRINSTQNLNYTCSVSYKSGGGVPVITENVSNREQSCFRNGTGINKPYQTGDVLLLSCYCRVTEPCGLTETVVGSMLGRGITPFEEEEYNNKKIRRIIVGPFNDTSIMTLRYDCSRGTTVESRCSTGPAQVSAKDFIINPTNPSVGSLECPVTEISTKYAETFSSTDGDTVIPTLDPTTASSTDKLSFIPILDSTTASSTDKPSLEGVNPPAKENNESFLTFIIVLVGIFALLIVSVAFLLVFIVLKRKKKGTKATKEGQEVHNLHQSIGNMDQTFNNLDQNADNQDQNVDIQDQNVDGHAYVGNVTDDSTDLPQLSDVSDGDVVVTDVMDGRDIEEYRGTLVSLPVDNNGFDNDDGEDPEDQFQTADTSDYSVLVAFMKRNNMQKEQDEVFGLDAEEDNLNQPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BQA6</accession>
    <name>A0A9Q1BQA6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_27709</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8031098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BQA6"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040291180" description="Secreted protein" evidence="1">
        <location>
            <begin position="18"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8031098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="139" mass="16026" checksum="4B5C0D9F4867D3D6" modified="2023-09-13" version="1">MLFGLFSIFCFLTFSSCAPKLDGKDHFDSKYASEVGIENLYVLPYIEVNNRPGVEQFLVPRKPGQIEAPWSEVQVKEDDARPYQLAGPFAQLIEGAERNFNIHRRTDSTVIWCGHHWTPIHRPSQRDTKSITTLYTMQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BQG3</accession>
    <name>A0A9Q1BQG3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Carbamoyl-phosphate synthase arginine-specific large chain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_27233</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BQG3"/>
    <dbReference type="OrthoDB" id="8186278at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004088">
        <property type="term" value="F:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CPAse_ATP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11405:SF53">
        <property type="entry name" value="CARBAMOYL-PHOSPHATE SYNTHASE [AMMONIA], MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11405">
        <property type="entry name" value="CARBAMOYLTRANSFERASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carbamoyl phosphate synthase ATP-binding" evidence="4">
        <location>
            <begin position="32"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8030741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="77" mass="8654" checksum="A0FDDE64BAB307F0" modified="2023-09-13" version="1">MALWCEDGLFWPYLVRFDQEVSFRLKARSLNEVVRDAADNCATVFNMENFDLLGVHAGGSIVVAPSQTLSNEAYQLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BR89</accession>
    <name>A0A9Q1BR89_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Sialidase-1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Lysosomal sialidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">N-acetyl-alpha-neuraminidase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">HOLleu_27905</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="22">Nanhai2018</strain>
            <tissue evidence="22">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Catalyzes the removal of sialic acid (N-acetylneuraminic acid) moieties from glycoproteins and glycolipids. To be active, it is strictly dependent on its presence in the multienzyme complex. Appears to have a preference for alpha 2-3 and alpha 2-6 sialyl linkage.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Interacts with cathepsin A (protective protein), beta-galactosidase and N-acetylgalactosamine-6-sulfate sulfatase in a multienzyme complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasmic vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Lysosome lumen</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Lysosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Lumenal side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the glycosyl hydrolase 33 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8031238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BR89"/>
    <dbReference type="OrthoDB" id="2739686at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043202">
        <property type="term" value="C:lysosomal lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004308">
        <property type="term" value="F:exo-alpha-sialidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006689">
        <property type="term" value="P:ganglioside catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009313">
        <property type="term" value="P:oligosaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15482">
        <property type="entry name" value="Sialidase_non-viral"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011040">
        <property type="entry name" value="Sialidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026856">
        <property type="entry name" value="Sialidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036278">
        <property type="entry name" value="Sialidase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10628">
        <property type="entry name" value="SIALIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10628:SF25">
        <property type="entry name" value="SIALIDASE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13088">
        <property type="entry name" value="BNR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50939">
        <property type="entry name" value="Sialidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14" id="KW-0458">Lysosome</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Sialidase" evidence="21">
        <location>
            <begin position="109"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00037235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00038519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00040509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00041332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00041413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF13088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KAJ8031238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="405" mass="45464" checksum="0C7F972C1E60AAFC" modified="2023-09-13" version="1">MGRGRYLSATWRMTVLTLRQGYQDWHVCLHFDSIAQYRSPDYRTDSHFKMAAKSSNYYSKALMFAVFLSSFHLTTTMNLLPIEEQLIWISNTDGVALFRTPILTLLPNGDLLAFVEARKYSSGDAGAKFIAMKRSTNQGLVWGPSEYILDDFHAQDGVNTGAVLFDSDTNTLFLLYSYCGHLANCVPGPDSQAAGSYMIASEDFGYSWSKPVNVKDLIPGVTSLEPIAFGPGYGLQKQHQPNKGRLIACGHTSEYVVRTMLCLYSDDHGKTWAVNKRFYSIPFNNTKVAHDLVPGEVQVVELPNGTLFMTVRNTHGYRCNCRMQMVSYDGGETFPLRNAFFDENLIDPNCFASMMRHKDMLFFSNPASTSHRVNMTVRWSLDYGSSWPGSVIIHEGGNRPKPAGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BSP5</accession>
    <name>A0A9Q1BSP5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">43 kDa receptor-associated protein of the synapse</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_25209</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RAPsyn family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BSP5"/>
    <dbReference type="OrthoDB" id="10040854at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031594">
        <property type="term" value="C:neuromuscular junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033130">
        <property type="term" value="F:acetylcholine receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043495">
        <property type="term" value="F:protein-membrane adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900075">
        <property type="term" value="P:positive regulation of neuromuscular synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007271">
        <property type="term" value="P:synaptic transmission, cholinergic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001237">
        <property type="entry name" value="Postsynaptic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052480">
        <property type="entry name" value="RAPsyn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019568">
        <property type="entry name" value="Rapsyn_myristoylation/link_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46574">
        <property type="entry name" value="43 KDA RECEPTOR-ASSOCIATED PROTEIN OF THE SYNAPSE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46574:SF1">
        <property type="entry name" value="43 KDA RECEPTOR-ASSOCIATED PROTEIN OF THE SYNAPSE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10579">
        <property type="entry name" value="Rapsyn_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00217">
        <property type="entry name" value="POSTSYNAPTIC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 8" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="repeat" description="TPR" evidence="8">
        <location>
            <begin position="164"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="9">
        <location>
            <begin position="365"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8031866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="419" mass="47641" checksum="81DF9FE7DAEF1C24" modified="2023-09-13" version="1">MGQANGKRDIEEGLRLFKAGKEERANRIWKQTLDRTKDRNIRFIMLRHLIGALYSMGRYRDCSSFAIEQLTAANIVDRKDYKAEAYWNLARANARSGEFQKALSFGKHALSNTSQKDNDGQLVGWIHLALAESHFGFSDFQGALEQSERALQQATKTDNLIMECETYVKFGEVYLALKDYDKAMKFFIRSSEMIRCLGQPWSPQLKSLVLLKLAEGNRKQGVLDRALQNCEEAMKIAVQELDYSMQAKCLATLGDIHRSRRDADRAHSKYEASWSQAGEIGDHVCQLHILMGLIKLFMSARDFEKAIEASEKGLEVGTGIGSKIHVLRCHWFLYQLYLQSESDRALSQDHAKKFDGLLRELQLYCGVCHDVIGKQKDNVYAMECCHIFHSKCVENSSFRSKGCPNCKVSSGILSKPYSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BU17</accession>
    <name>A0A9Q1BU17_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">B box-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_26245</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BU17"/>
    <dbReference type="OrthoDB" id="10113904at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19756">
        <property type="entry name" value="Bbox2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047153">
        <property type="entry name" value="TRIM45/56/19-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25462">
        <property type="entry name" value="BONUS, ISOFORM C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25462:SF296">
        <property type="entry name" value="MEIOTIC P26, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101898">
        <property type="entry name" value="NHL repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B box-type" evidence="2">
        <location>
            <begin position="5"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="159"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8032663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="551" mass="63771" checksum="3EA3B422F237A266" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BY48</accession>
    <name>A0A9Q1BY48_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SCAN box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_21958</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BY48"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF13">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCAN box" evidence="2">
        <location>
            <begin position="18"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="112"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8034930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="137" mass="16209" checksum="E2B34A95618A2911" modified="2023-09-13" version="1">MDYDKVKKAILQAYELVPEAYRQKFRKYRKADTQTYVEFAYQKERHFDRWCASKKVSTFDTLRQLMLVEEFKRCVNDDIKTHLEENKADNLKEVANLADQYALTHKFSKVGQTQNKGQFSRPNKKYGNSSGTTGQMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BYH3</accession>
    <name>A0A9Q1BYH3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">E-selectin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_22270</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BYH3"/>
    <dbReference type="OrthoDB" id="406096at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050350">
        <property type="entry name" value="Compl-Cell_Adhes-Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19325">
        <property type="entry name" value="COMPLEMENT COMPONENT-RELATED SUSHI DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19325:SF560">
        <property type="entry name" value="SUSHI, VON WILLEBRAND FACTOR TYPE A, EGF AND PENTRAXIN DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 5" id="KW-0768">Sushi</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="7">
        <location>
            <begin position="79"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="7">
        <location>
            <begin position="146"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="7">
        <location>
            <begin position="326"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="7">
        <location>
            <begin position="393"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="7">
        <location>
            <begin position="460"/>
            <end position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8035140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="539" mass="60162" checksum="16F519BD29AA3459" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BZR8</accession>
    <name>A0A9Q1BZR8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">TRAF3-interacting protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_19485</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Cilium axoneme</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium basal body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the TRAF3IP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8035722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BZR8"/>
    <dbReference type="OrthoDB" id="10258914at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030992">
        <property type="term" value="C:intraciliary transport particle B"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042073">
        <property type="term" value="P:intraciliary transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070507">
        <property type="term" value="P:regulation of microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.50">
        <property type="entry name" value="Microtubule-binding protein MIP-T3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018799">
        <property type="entry name" value="TRAF3IP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041476">
        <property type="entry name" value="TRAF3IP1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040468">
        <property type="entry name" value="TRAF3IP1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042576">
        <property type="entry name" value="TRAF3IP1_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31363">
        <property type="entry name" value="TRAF3-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31363:SF0">
        <property type="entry name" value="TRAF3-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10243">
        <property type="entry name" value="MIP-T3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17749">
        <property type="entry name" value="MIP-T3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="5 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TRAF3-interacting protein 1 N-terminal" evidence="11">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="TRAF3-interacting protein 1 C-terminal" evidence="12">
        <location>
            <begin position="416"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="69"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="486"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="69"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="94"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="315"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="358"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="374"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="420"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00043971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF10243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF17749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8035722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="570" mass="64935" checksum="DBE75474FA777AF3" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C129</accession>
    <name>A0A9Q1C129_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cysteine desulfurase 1, chloroplastic</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_20666</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C129"/>
    <dbReference type="OrthoDB" id="420046at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43686:SF1">
        <property type="entry name" value="AMINOTRAN_5 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43686">
        <property type="entry name" value="SULFURTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotransferase class V" evidence="2">
        <location>
            <begin position="217"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="696"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="726"/>
            <end position="735"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8036632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="735" mass="82977" checksum="D7B5595DD6818DDA" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C247</accession>
    <name>A0A9Q1C247_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ethanolamine-phosphate cytidylyltransferase</fullName>
            <ecNumber evidence="10">2.7.7.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">CTP:phosphoethanolamine cytidylyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_18011</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="9">Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from ethanolamine: step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytidylyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.14" evidence="10"/>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C247"/>
    <dbReference type="OrthoDB" id="40021at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004306">
        <property type="term" value="F:ethanolamine-phosphate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006646">
        <property type="term" value="P:phosphatidylethanolamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02174">
        <property type="entry name" value="CCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02173">
        <property type="entry name" value="ECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041723">
        <property type="entry name" value="CCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044608">
        <property type="entry name" value="Ect1/PCYT2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45780">
        <property type="entry name" value="ETHANOLAMINE-PHOSPHATE CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45780:SF2">
        <property type="entry name" value="ETHANOLAMINE-PHOSPHATE CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5 13" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="7" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cytidyltransferase-like" evidence="12">
        <location>
            <begin position="19"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Cytidyltransferase-like" evidence="12">
        <location>
            <begin position="213"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8037241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="388" mass="43900" checksum="2FFB1053E9FDC20F" modified="2023-09-13" version="1">MTEQKPSSIPTEKKQVRVWADGCFDLAHFGHANSLRQAKKMGDYLIVGVHSDDAIREHKGPPVMTEQERYKVIRAIKWVDEVVEDAPYVTLLEYLDKYQCDFCVHGNDITLDADGNDTYRIVKDAGRYREVERTAGVSTTDIVGRMLLLTKTHHLPDAVLNENAETTEANAASASCSTMRSPYTGISQFLPSTRKIVQFAEGKEPMPGDKIVYCPGAFDLFHVGHVDFLQKASQMGDYVIVGLYTDKEVNRTENSNYPIMNLQERTLGVLANRYVSEVVIGAPYKITKELLEHFNVDIVVRGKTSYNSCADSTDPFEVPKQMGIYHEVDSGNDMSTQKIVARIIANSITFRERNKKKEAKEMRNMKLMEERKLKEINANQPEPQVIDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C3M3</accession>
    <name>A0A9Q1C3M3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Growth factor receptor-bound protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_18497</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C3M3"/>
    <dbReference type="OrthoDB" id="10255964at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="CDD" id="cd09941">
        <property type="entry name" value="SH2_Grb2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11805">
        <property type="entry name" value="SH3_GRB2_like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000072">
        <property type="entry name" value="Unconventional Myosin IB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043539">
        <property type="entry name" value="Grb2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46037">
        <property type="entry name" value="PROTEIN ENHANCER OF SEVENLESS 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00499">
        <property type="entry name" value="P67PHOX"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="1 4" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="6">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="5">
        <location>
            <begin position="60"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="6">
        <location>
            <begin position="166"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8037628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="226" mass="26239" checksum="E936F9AF89C432B4" modified="2023-09-13" version="1">MEYVAKFDFTPSQEDELAFRKGDIVKVIDKKKDKNWYKAELKGRQGLVPKNYIEGKPHDWYVGHVRRKVTEDFLMKQLEDGTFVVRDSESSPGDFSLSVKYAGQVQHFKVLVDGAGKYFIWVVKFNSLNELVEYHRTNSISRTAKIFLKDVDSYALQSFKSGQGDPTSIRVRAKYDFDPQEQGELSFRFGDTIRVLEQTDANWWKGECRGRTGMFPTPYVEVLNDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-02-05" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C5E7</accession>
    <name>A0A9Q1C5E7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Guanine nucleotide exchange factor VAV3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_15921</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45818">
        <property type="entry name" value="PROTEIN VAV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45818:SF3">
        <property type="entry name" value="PROTEIN VAV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="1 3" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH2" evidence="5">
        <location>
            <begin position="763"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="5">
        <location>
            <begin position="1350"/>
            <end position="1427"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="6">
        <location>
            <begin position="1458"/>
            <end position="1525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="305"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1003"/>
            <end position="1025"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1317"/>
            <end position="1341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1015"/>
            <end position="1025"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8038484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1526" mass="173342" checksum="D06E382888496F3F" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C5Q6</accession>
    <name>A0A9Q1C5Q6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_16133</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C5Q6"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.130.10">
        <property type="entry name" value="Ribonuclease A-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036816">
        <property type="entry name" value="RNaseA-like_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040177785" evidence="1">
        <location>
            <begin position="25"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8038649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="155" mass="17925" checksum="9E0015268A8DFED8" modified="2023-09-13" version="1">MKNFAVICFLVAVTAVLFVQRISGEDGFWYGEELHDVEKRQTWNAFCDKHVVEAPGTKKDANVWNQIARERLGTDYGVRLSNTFIRVQDKNLILTSFFRRRECSNGYTKSDKKFSLVEMTWNAASGKYSVNFPGEWYICVKVAANRQPLDFHGRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C5Q7</accession>
    <name>A0A9Q1C5Q7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">GCS light chain</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Gamma-ECS regulatory subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Gamma-glutamylcysteine synthetase regulatory subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Glutamate--cysteine ligase modifier subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_16164</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterodimer of a catalytic heavy chain and a regulatory light chain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the aldo/keto reductase family. Glutamate--cysteine ligase light chain subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C5Q7"/>
    <dbReference type="OrthoDB" id="5596051at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017109">
        <property type="term" value="C:glutamate-cysteine ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035226">
        <property type="term" value="F:glutamate-cysteine ligase catalytic subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006750">
        <property type="term" value="P:glutathione biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032963">
        <property type="entry name" value="Gclm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13295">
        <property type="entry name" value="GLUTAMATE CYSTEINE LIGASE REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13295:SF4">
        <property type="entry name" value="GLUTAMATE--CYSTEINE LIGASE REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0317">Glutathione biosynthesis</keyword>
    <keyword evidence="10" id="KW-0436">Ligase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="9">
        <location>
            <begin position="79"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00030406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8038675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="273" mass="31193" checksum="7BF24C0BDE0BB256" modified="2023-09-13" version="1">MDVPRIPEASSLIISSGNIINWECLKARKRIAQSTTEEVKDCLNAVLNSSLKAVNWEKARLEKSLEYSVPKCSPTLANLEESREELKVTVKAMMCNTEPEVIVNAIQKVMTELHTETIDVLLLSVSERPNALETFHMDHLKTLWSVLERMVENNQIKMLGVCDLDKQGLEELYNWAKVKPSINQVNEVSCCIIPPDLNAFTKENKIQLLTHGDNPEILPAEDLQEIIRNSFSDMDAHRWHPSWVMRYTALFRCRGVIARKGYIVNAQRNLDHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C658</accession>
    <name>A0A9Q1C658_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Cytochrome P450 1A1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HOLleu_16429</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Nanhai2018</strain>
            <tissue evidence="16">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C658"/>
    <dbReference type="OrthoDB" id="1470350at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004508">
        <property type="term" value="F:steroid 17-alpha-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042446">
        <property type="term" value="P:hormone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042448">
        <property type="term" value="P:progesterone metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000238">
        <property type="entry name" value="Cytochrome P450 2A6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24289">
        <property type="entry name" value="STEROID 17-ALPHA-HYDROXYLASE/17,20 LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24289:SF20">
        <property type="entry name" value="STEROID 17-ALPHA-HYDROXYLASE_17,20 LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 13 14" id="KW-0349">Heme</keyword>
    <keyword evidence="10 13 14" id="KW-0408">Iron</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0492">Microsome</keyword>
    <keyword evidence="11 14" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="9 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="13">
        <location>
            <position position="444"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ8038874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="501" mass="56739" checksum="B07403FA76E832C7" modified="2023-09-13" version="1">MDGTLVESHVVVATLILSIVGLFWHWSTKKPSKDFPPGPRPLPLVGNLLDFAFKPPHMVFLELRNKYGDIFSVKIGQRWTVILNSLHATREALVKQPVNFAGRPDLYSFKLLSGDGKSIALSDYSIDWANNRKMIHASLRNLVTGDHTKFEALILEFTPQVASALDLTKGKPFDPKQLICHLVYSILGKICFGESFAFDDPMLLEKMAAGQEFLELVGNGLPIDFFPSLAWIPFKRAEKLRNLCELNFLDIHRAIDCHRKTFVPGKPRDFIDQMLDAQLAAKQNGEDTNISDEVIATGIADIHGGGTDTTIVTLYWSLALMVQFPQVQDKIAQLADEVIGRERLPTLADREKLPYIVAAVHEVLRYSTKLPMSVPHSTTQDCKLDGYFIPKGTMVMTNLWAIHRDGEHWEQPYSFRPEHFLDETGTVRKHPEGFVPFSTGRRVCIGESMVKAELFLLFTWLFQHFKFSKVPGTEDQSYIEHGQILVANEVNTYKLIAKRRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C893</accession>
    <name>A0A9Q1C893_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Carbohydrate sulfotransferase 15</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_11845</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C893"/>
    <dbReference type="OrthoDB" id="8068875at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050659">
        <property type="term" value="F:N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019319">
        <property type="term" value="P:hexose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052654">
        <property type="entry name" value="CS_Sulfotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15723">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15723:SF0">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="domain" description="Sulfotransferase" evidence="2">
        <location>
            <begin position="279"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8041118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="453" mass="52668" checksum="EA99D793C4108339" modified="2023-09-13" version="1">MSFRITGNMKLLKIYLGWIIVFCLVFGVAIWLTNEGRYFNEKRHAVAYASSNFHLKDQANFKRVASEENNTEDTSLSRISNHDTITRVITKQSQRLPLFTKEYLETVAPEIFNRVPEHFLPEYRNPCWLENGTLWCLPYFYIIGFHKCGTTDLFDKLLFHPSVLKVGKEPHWWAIRRLGLKKHFSLHADIIKEVQALPGAQDVSSFAWYLNWFKYQTVAHVQGSSLSIKDSNGTPKTFHSKVFGDGSVSTLTRVIFEPWDKVFPGGEDPDLLPYLMHAIQPQAKIIITLRDPVSRFKSMYYRKFAHGSSPTSLHQYAVLAVRCATKCLKESGVRHCAYFSHCQNLPQIYLGIYVAFIKHWATVYGLENMLILRMEDKLKHPVSEFRKVLNYLELENLSDAECERIMNTKVFNQNKAGHQEMLNETKALLREFYKPFNKQLATFLNDEMYLFGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C8I1</accession>
    <name>A0A9Q1C8I1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phospholipase A2</fullName>
            <ecNumber evidence="8">3.1.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_14383</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1-acyl-sn-glycero-3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15801"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="5">Binds 1 Ca(2+) ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the phospholipase A2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.4" evidence="8"/>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C8I1"/>
    <dbReference type="OrthoDB" id="5841574at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047498">
        <property type="term" value="F:calcium-dependent phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050482">
        <property type="term" value="P:arachidonate secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00125">
        <property type="entry name" value="PLA2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.90.10:FF:000015">
        <property type="entry name" value="Phospholipase A(2)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.90.10">
        <property type="entry name" value="Phospholipase A2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001211">
        <property type="entry name" value="PLA2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016090">
        <property type="entry name" value="PLA2-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033112">
        <property type="entry name" value="PLA2_Asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033113">
        <property type="entry name" value="PLA2_histidine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036444">
        <property type="entry name" value="PLipase_A2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11716:SF51">
        <property type="entry name" value="PHOSPHOLIPASE A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11716">
        <property type="entry name" value="PHOSPHOLIPASE A2 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00068">
        <property type="entry name" value="Phospholip_A2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00389">
        <property type="entry name" value="PHPHLIPASEA2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00085">
        <property type="entry name" value="PA2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48619">
        <property type="entry name" value="Phospholipase A2, PLA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00119">
        <property type="entry name" value="PA2_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00118">
        <property type="entry name" value="PA2_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0106">Calcium</keyword>
    <keyword evidence="3 6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040530610" description="Phospholipase A2" evidence="8">
        <location>
            <begin position="25"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipase A2-like central" evidence="9">
        <location>
            <begin position="47"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="96"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="161"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="77"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="92"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="99"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="109"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR601211-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR601211-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR601211-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8040163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="204" mass="23041" checksum="B724DB2E082DD65F" modified="2023-09-13" version="1">MAVLRSFLAVIVLVTLDLFGRSYGGVITLNTTQTDQKIRTAPRKLNALWELGSMIKCTTGKDLFRAIFDYAWYGCYCGFGGKGVPVDGTDRCCMIHDNCYDVAFKSHDCPFQPEVYYILYRRHFHMRNCLTPQANITCDAASAYPSWFRLSHCAASICKCDAAVSMCIGRNPFNRSHAFYPKRTCRKGRAPTISPVVENEVTTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CBU2</accession>
    <name>A0A9Q1CBU2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Retinoblastoma-like protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HOLleu_12594</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Nanhai2018</strain>
            <tissue evidence="15">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the retinoblastoma protein (RB) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CBU2"/>
    <dbReference type="OrthoDB" id="844594at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000134">
        <property type="term" value="P:negative regulation of G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000035">
        <property type="entry name" value="RB transcriptional corepressor-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002720">
        <property type="entry name" value="RB_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002719">
        <property type="entry name" value="RB_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015030">
        <property type="entry name" value="RB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028309">
        <property type="entry name" value="RB_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024599">
        <property type="entry name" value="RB_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13742:SF17">
        <property type="entry name" value="RE32990P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13742">
        <property type="entry name" value="RETINOBLASTOMA-ASSOCIATED PROTEIN RB -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11934">
        <property type="entry name" value="DUF3452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01858">
        <property type="entry name" value="RB_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01857">
        <property type="entry name" value="RB_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01367">
        <property type="entry name" value="DUF3452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01368">
        <property type="entry name" value="RB_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01369">
        <property type="entry name" value="Rb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Retinoblastoma-associated protein N-terminal" evidence="12">
        <location>
            <begin position="60"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="Retinoblastoma-associated protein A-box" evidence="13">
        <location>
            <begin position="387"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="11">
        <location>
            <begin position="812"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="domain" description="Retinoblastoma-associated protein C-terminal" evidence="14">
        <location>
            <begin position="981"/>
            <end position="1104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="628"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="769"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="901"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="981"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1058"/>
            <end position="1105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="662"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="697"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="901"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="932"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1068"/>
            <end position="1082"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1083"/>
            <end position="1105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ8041704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1105" mass="124237" checksum="C4F8935910E865F5" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CCJ0</accession>
    <name>A0A9Q1CCJ0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_13460</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8042411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CCJ0"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8042411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="72" mass="8020" checksum="CA460E0E4DA8A09E" modified="2023-09-13" version="1">MPYSERKNPIIFGGVQRSSGVTGGQTLKTLLTQYLNMHCGENMNTIIFGGGQRSSWVNADQTLKSLLTRIFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CCM0</accession>
    <name>A0A9Q1CCM0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Hyalin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_13537</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8042472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CCM0"/>
    <dbReference type="OrthoDB" id="5948052at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003410">
        <property type="entry name" value="HYR_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24273">
        <property type="entry name" value="FI04643P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24273:SF32">
        <property type="entry name" value="HYALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02494">
        <property type="entry name" value="HYR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50825">
        <property type="entry name" value="HYR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="HYR" evidence="2">
        <location>
            <begin position="20"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="HYR" evidence="2">
        <location>
            <begin position="105"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="HYR" evidence="2">
        <location>
            <begin position="190"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="HYR" evidence="2">
        <location>
            <begin position="275"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8042472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="377" mass="39156" checksum="555FA1823D9B6DA3" modified="2023-09-13" version="1">MCENELFCMDVIFELLLFLGDDVSPVVDCSGEGIMLTAPAGASGVTVETLPRCRATDNSGQVNFVSQSPSPGSFFLIGDTMVTVVYRDPSGNTGVGFFTVTVRAGDDVSPVVDCSGEGIMLTAPAGASGVTVETLPRCRATDNSGQVNFVSQSPSPGYFFLIGDTMVTVVYRDPSGNTGVGFFTVTVRAGDDVSPVVDCSGEGIMLTTPAGASGVTVETLPRCRATDNSGQVNFVSQSPSPGSFFLIGDTMVTVVYRDPSGNTGVGFFTVTVRAGDDVSPVVDCSGEGIMLTAQAGASGVTVETLPRCRATDNSGQVNFVSQSPSPGSFFLIGDTMVTVVYRDPSGNTGVGFFTVTVRAGKFLPSFITSLFKCNLKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CFL5</accession>
    <name>A0A9Q1CFL5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_11077</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CFL5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8043808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="68" mass="7886" checksum="A0490AB60C6B6BDC" modified="2023-09-13" version="1">MAEFRESRELEDFQLSGERKIRTEMRKEQTTSEAGSVGKRLQRQLSSGPHVNFSSLSITEVFFTEDFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CG50</accession>
    <name>A0A9Q1CG50_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Monocarboxylate transporter 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_07074</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CG50"/>
    <dbReference type="OrthoDB" id="410267at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008028">
        <property type="term" value="F:monocarboxylic acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF303">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="121"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="152"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="212"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="248"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="276"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="302"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="336"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8044346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="370" mass="40726" checksum="7B80921094A590C5" modified="2023-09-13" version="1">MKANGVILPYVVEKLQTSHTLVAWAFTLQCSLGFLINDLPKFLFCEAPLSKQLLHYFSYRQLGIVGGFLAGFGYIGSGLLTSSILDHFLFHSMSGFGFGLLCIPSFMYIHAHVDYQTFAKIQSTAGIFIYVGVAILPLVMKWLIDIYGHNDGLAIFGALIWNCVLTGVAMRPTESSSLEIESTSCSTVPREKLHVSDKYLPLLSPLFRHRNFAICMLFVTSGMYVYSSWAIFLVSFGNDLGFSSQQSVYLSTAGGVGGAVGSLLPLVLFSYNKMNAYTTCLFPSVANGITLLLSTFMTDFTIVFYLMLLSGFIQGFQYAGICGFIPLLLCEHHLGYGAVLYFLVEGVLYQFGGLLPGIAHVKEKEKENLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CJ71</accession>
    <name>A0A9Q1CJ71_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">ER membrane protein complex subunit 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_05114</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the EMC3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CJ71"/>
    <dbReference type="OrthoDB" id="6745403at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072546">
        <property type="term" value="C:EMC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034975">
        <property type="term" value="P:protein folding in endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008568">
        <property type="entry name" value="EMC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002809">
        <property type="entry name" value="EMC3/TMCO1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13116">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13116:SF5">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01956">
        <property type="entry name" value="EMC3_TMCO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF010045">
        <property type="entry name" value="DUF850_TM_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01415">
        <property type="entry name" value="DUF106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR010045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8046442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="255" mass="29510" checksum="13D9570F85B8D08C" modified="2023-09-13" version="1">MAELLLDTNIRLWVVLPIVFITFFVGIIRHYVSLLLSSQKKVDLQKVSDGQVLLRSKMLRENGHYLPKKSFQMRKHFFNDEDSGFFKTQKREAAMQNPMADPSMLTEMMKGNLTNILPMIVIGGWINWTFSGFVITKVPFPLTLRFKPMLQRGVELASLDASWVSSASWYFLNVFGLRSVYSLILGENNAADQTRLMQQQMAQPMAMAPDPSKAFKAEWEALEIVDHKWKLEKVEDELICQTPYQEDVYVKNKYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CJB9</accession>
    <name>A0A9Q1CJB9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Protein O-mannosyl-transferase 2</fullName>
            <ecNumber evidence="4">2.4.1.109</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HOLleu_08826</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Nanhai2018</strain>
            <tissue evidence="17">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + L-seryl-[protein] = 3-O-(alpha-D-mannosyl)-L-seryl-[protein] + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17377"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:13546"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137321"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + L-threonyl-[protein] = 3-O-(alpha-D-mannosyl)-L-threonyl-[protein] + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53396"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:13547"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137323"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase 39 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.109" evidence="4"/>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CJB9"/>
    <dbReference type="OrthoDB" id="5561486at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004169">
        <property type="term" value="F:dolichyl-phosphate-mannose-protein mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd23282">
        <property type="entry name" value="beta-trefoil_MIR_POMT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000026">
        <property type="entry name" value="Blast:Protein O-mannosyl-transferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003342">
        <property type="entry name" value="ArnT-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036300">
        <property type="entry name" value="MIR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016093">
        <property type="entry name" value="MIR_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027005">
        <property type="entry name" value="PMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032421">
        <property type="entry name" value="PMT_4TMC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10050">
        <property type="entry name" value="DOLICHYL-PHOSPHATE-MANNOSE--PROTEIN MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10050:SF46">
        <property type="entry name" value="PROTEIN O-MANNOSYL-TRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02815">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02366">
        <property type="entry name" value="PMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16192">
        <property type="entry name" value="PMT_4TMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00472">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82109">
        <property type="entry name" value="MIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50919">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="15"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="105"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="157"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="188"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="242"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="560"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="600"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="631"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="664"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="16">
        <location>
            <begin position="297"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="16">
        <location>
            <begin position="368"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="16">
        <location>
            <begin position="429"/>
            <end position="485"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00039583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00045102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ8045755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="713" mass="81170" checksum="FB3395A0AE572D21" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CJN2</accession>
    <name>A0A9Q1CJN2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Retinol dehydrogenase 13</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_09098</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CJN2"/>
    <dbReference type="OrthoDB" id="191139at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43157:SF31">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS F PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43157">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS F PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040288568" evidence="3">
        <location>
            <begin position="18"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8045968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="316" mass="34585" checksum="77C92F73A29B86E4" modified="2023-09-13" version="1">MNTAGFGLLLLKRWVAGGWCYSKARLDGKTVLITGANTGIGKETAIDLAKRGAKVILACRNVDKAKSAQADIIRLSGNNNISVIELDLASLDSVRQCAQKIIEGVPRLDILINNAGVMACPRWETKDGFEMQLGTNHLGHFLFTNLLLDLIKKSAPARIINLSSMAHEGGDIFFDDLMLTKDYTAIKSYCQSKLANVLFSSELARRLEGTGITTYSLHPGGVDTDLQRHVGTTWPWARIPFMIMGPFIRTFLFKTALQGAQTSIYCAVDEKAGQETGLYYSDCAVKKPSPKALDVTVAKKLWEVSAELVGLEKKVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CJW8</accession>
    <name>A0A9Q1CJW8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SCAN box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_05661</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CJW8"/>
    <dbReference type="OrthoDB" id="10000497at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF13">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCAN box" evidence="3">
        <location>
            <begin position="238"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="331"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="78"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="331"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="347"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="371"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="381"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8046847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="390" mass="45621" checksum="74981E1A0DD9652C" modified="2023-09-13" version="1">MPFNLDKFVASPSVEELDSLKKSEIVKVAKHYGVEFQPLMRKDEIKRYVLEYLVDESILPITVLETAITVPTDNTFELKRLEIEMNKEIRLKEMEREREREEREMQMQMQKEKEEREMQMQMQREKEAREHEFRLKQLELGVIKGSDPKIGLDTGGLFDVSKHVKFVPKFQEDNVEKFFNHFEKLGEQLKWPRDKWSILIQSNFTGKAQEVYSALSIEDSMDYDKVKKAILQAYELVPEAYRQKFRKYRKADTQTYVEFAYQKERHFDRWCASKKVSTFDTLRQLMLVEEFKRCVNDDIKTHLEENKADKLSEVANLADQYALTHKFGKVGQTQNKGQFSRPNKKYGNNSGTTGNSGSQSGSISPTNSGKKQPDSSRQKNDQVSSNKAAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CK59</accession>
    <name>A0A9Q1CK59_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SCAN box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_05882</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CK59"/>
    <dbReference type="OrthoDB" id="6077919at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF1">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="SCAN box" evidence="2">
        <location>
            <begin position="197"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8047012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="320" mass="36867" checksum="2CC458CC78F92583" modified="2023-09-13" version="1">MLLKSSLKLYFQSDVEKLKIFFVLLLSLEIALLVSSTCTLVGRKMDFEKYITLGKESGLKGDALIEFAREREKIARDERQKEREHQKEMKEYDLKIASTGSNLADSKGGLGNTAPTKLPKLPLFKEGTDDMDAFILRFERFATAANWPRTIWATSMGALLTGRALEVYSRMSDSQSKDYAKLKSALLFKFQLTADGFRGKFRNSRCESRETYSQYLERIKSYLIRWIEMSKKQKTYDDLLDLLLQEQMINSSSKELAVFLKERTPDKASDMALLADKYREAYGNYDKGIRVLRIGLETKMKMIKMELLKAQAKIVLVTKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CKS1</accession>
    <name>A0A9Q1CKS1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_04883</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CKS1"/>
    <dbReference type="OrthoDB" id="10071925at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="21"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="476"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="332"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="121"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="153"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="222"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="241"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="267"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="301"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="481"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8046264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="522" mass="58675" checksum="14D35BE7E303B14A" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CMK8</accession>
    <name>A0A9Q1CMK8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_00712</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CMK8"/>
    <dbReference type="OrthoDB" id="5814287at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31751">
        <property type="entry name" value="SI:CH211-108C17.2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31751:SF44">
        <property type="entry name" value="SI:CH211-211K8.4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8048407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="114" mass="13113" checksum="C0330E55E6F9C81D" modified="2023-09-13" version="1">MWTSVVHHVCDIHTWNSGNLFHSCKHENLSQEARNQKMWLKPGSKAHEALKNVVFNKTLRRDLKQVTKACHTGHLEVFHSALLKYCPKRLHFNYPTNAGAGAREVVNDELKKSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CNQ6</accession>
    <name>A0A9Q1CNQ6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_00988</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CNQ6"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8048611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="78" mass="9337" checksum="78C1CE37CB66B8BA" modified="2023-09-13" version="1">MPGNGTDIGTTIEWSGLYGYRLNMNSGQWEHDDGRKEMAVMTDENYREEEQWIVNLQSRSLPQLYYLLFLFCHFEYFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CPR4</accession>
    <name>A0A9Q1CPR4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">TIR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_01009</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CPR4"/>
    <dbReference type="OrthoDB" id="6078042at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00045">
        <property type="entry name" value="DED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001875">
        <property type="entry name" value="DED_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47508:SF1">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47508">
        <property type="entry name" value="SAM DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13676">
        <property type="entry name" value="TIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50168">
        <property type="entry name" value="DED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DED" evidence="2">
        <location>
            <begin position="8"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="TIR" evidence="1">
        <location>
            <begin position="144"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8048623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="289" mass="32911" checksum="CE13889E018C19AE" modified="2023-09-13" version="1">MNKGQSKIHISLLQDLANDLTSETFLKLKERCKAETYGRLGAGQLERIQTLTQLFEKLQQKKVFYQGNYANLKSLLRECEAEDAVDTVKIAEKELFEAGYSVEGYQQPDHAVPIPTPAPPGKSPAGLCKADNHFSRLRQRKDEKAEKIFISFSFTMKDRVTELKDVIEEYIGKDTCWICTTGVRGGDNLDEKISQAINNAEVVLCMISKAYADSADCEKEFLLSRNLGKHIIPVLIEQVRWPLVTSDGQASSMQLGWARDLYVNMYDENKMEEEFSKLIEQIKRQLQMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CR15</accession>
    <name>A0A9Q1CR15_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_02960</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CR15"/>
    <dbReference type="OrthoDB" id="10401725at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8049972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="177" mass="20279" checksum="61635BDB7D9ECFD1" modified="2023-09-13" version="1">MDEEGTVMEVHLVSIGKQTAKRLAQKLHKRKFYIITHFSLNKREECTYLRLSADKAKIFQTAKQFTLPQEKVQEFFHPAQCTIPHALASPKKRRLSVGGTIAQVYELKQGSNWKRRDVLLSDNSTGKKICCKLWGDHATKITETNKGSTVTIENVEVDVYNDNHQLRSTNMTTIQVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CS48</accession>
    <name>A0A9Q1CS48_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Pleckstrin-likey domain-containing family F member 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_02691</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CS48"/>
    <dbReference type="OrthoDB" id="70570at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007032">
        <property type="term" value="P:endosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008333">
        <property type="term" value="P:endosome to lysosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15717">
        <property type="entry name" value="FYVE_PKHF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01218">
        <property type="entry name" value="PH_Phafin2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000167">
        <property type="entry name" value="Pleckstrin homology domain-containing family F member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051765">
        <property type="entry name" value="PH_domain-containing_F"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037871">
        <property type="entry name" value="PH_Phafin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055251">
        <property type="entry name" value="SOS1_NGEF_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46280:SF3">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY DOMAIN-CONTAINING FAMILY F MEMBER 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46280">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY DOMAIN-CONTAINING FAMILY F MEMBER 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22697">
        <property type="entry name" value="SOS1_NGEF_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PH" evidence="6">
        <location>
            <begin position="35"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="FYVE-type" evidence="7">
        <location>
            <begin position="152"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="215"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="219"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="231"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8049790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="261" mass="29508" checksum="6768A2A3E74A64A5" modified="2023-09-13" version="1">MVDRLVHSPANRKRISVVEDRFGSSGQPLAVPGRVLVGEGVLTKLCRKKTKPRMFFLFNDILVYGNIVIHKKKYNKQHILPLEDVKLKSVDDDGVMKNAWKIISATKSFMVSAATATEKSEWMAHINKCIQDLLTKSGKKPATDLSPVWVPDSEAPVCMSCHKNKFTAINRRHHCRKCGYVICSSCSTKRYLLPQIASRPIRVCDTCYNTLSAGQTGEDAGRSYQDRDSSGEDDDDDDDEEDDSQKTEEEVHPTFYEDNYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CSL2</accession>
    <name>A0A9Q1CSL2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_03447</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CSL2"/>
    <dbReference type="OrthoDB" id="10035704at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47018">
        <property type="entry name" value="CXC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47018:SF3">
        <property type="entry name" value="MYCBP-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8050301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="376" mass="43272" checksum="7606ECB733A82A3F" modified="2023-09-13" version="1">MDEATYAKAQQIRWSSEVFRNRVVMRLGEFHVSMAYLACLGSRFGDAGLRDLLIESDIVAQGSINGVLSGHHYNRSVRTHKLTADALSRLRWLAFLDTLNDEQRDEIRKIIVDLHENYLSNNILAFVEIDEVKMLLQQFQRFVNIQSAVNPTFAFWSSYLEMVQLLMLFIRATREGNWELHLSTVRSMLPWFFACDRVHYSRYLHVYWLEMKNLRERHPDVHEKLSEGEFVVQRQDQYGFSQIPCDQTIEQTCNRDTKTKGGLTGFTLNRDAVHRWILSSHERAAITKECHVMAGKFAQSRKKDLDRTRSCKDEEHTKSVMATVGSMINPFEPGRQELVNISSGVVAPDNVCTDLLSAEELGEIAVKAYCKETAEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1GXR6</accession>
    <name>A0A9Q1GXR6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_31100</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1GXR6"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="195"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="255"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="285"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="339"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="375"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="405"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="436"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="492"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="521"/>
            <end position="541"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8028767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="563" mass="59560" checksum="3CF8C10789F669BD" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1GY20</accession>
    <name>A0A9Q1GY20_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Peroxisomal (S)-2-hydroxy-acid oxidase GLO5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_32577</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1GY20"/>
    <dbReference type="OrthoDB" id="25826at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000262">
        <property type="entry name" value="FMN-dep_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037396">
        <property type="entry name" value="FMN_HAD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578:SF146">
        <property type="entry name" value="OXIDASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578">
        <property type="entry name" value="S -2-HYDROXY-ACID OXIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01070">
        <property type="entry name" value="FMN_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51349">
        <property type="entry name" value="FMN_HYDROXY_ACID_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FMN hydroxy acid dehydrogenase" evidence="3">
        <location>
            <begin position="7"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8027433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="264" mass="29412" checksum="1DC2EEC05898F6FA" modified="2023-09-13" version="1">MEHGIIPTSVNEFEKLAEEKMAKDKKHRALWCYISLGTGDKQTLEDSKAAFRRFRFQPRILTAKMERSTQTTLLGNPVSLPIGISPVAGYSSLHPYGATLAGKAAARAGAVMILGSVGNISLEDFAERVPSDGLYWAQTYLFRDKRNTLRLVRNAERLGFKALVVTVDSPVEHKGAGSNKETMDEYTRTVVETPAANIRYVNGKEREISPASLSEVWTEAYKEVFCCANWEDLSWLQSETKLPIILKGVLSGEFHFKNSFSIVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H055</accession>
    <name>A0A9Q1H055_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Junction-mediating and -regulatory protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_28195</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8028934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H055"/>
    <dbReference type="OrthoDB" id="6284683at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071933">
        <property type="term" value="F:Arp2/3 complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034314">
        <property type="term" value="P:Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031738">
        <property type="entry name" value="JMY/WHAMM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031808">
        <property type="entry name" value="JMY/WHAMM_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23330">
        <property type="entry name" value="P300 TRANSCRIPTIONAL COFACTOR JMY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23330:SF10">
        <property type="entry name" value="WH2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15871">
        <property type="entry name" value="JMY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15920">
        <property type="entry name" value="WHAMM-JMY_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="4 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="JMY/WHAMM N-terminal" evidence="9">
        <location>
            <begin position="8"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="JMY/WHAMM middle" evidence="8">
        <location>
            <begin position="176"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="495"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="669"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="391"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="454"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="495"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="593"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="640"/>
            <end position="650"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF15871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF15920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8028934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="750" mass="86447" checksum="96F7A7419E6151A7" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H0D6</accession>
    <name>A0A9Q1H0D6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcription factor SPT20-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_31021</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SPT20 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H0D6"/>
    <dbReference type="OrthoDB" id="1932706at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021950">
        <property type="entry name" value="Spt20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046468">
        <property type="entry name" value="Spt20-like_SEP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13526">
        <property type="entry name" value="TRANSCRIPTION FACTOR SPT20 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13526:SF8">
        <property type="entry name" value="TRANSCRIPTION FACTOR SPT20 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12090">
        <property type="entry name" value="Spt20_SEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Spt20-like SEP" evidence="4">
        <location>
            <begin position="75"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="408"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="450"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="501"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="597"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="929"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="877"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="980"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="468"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="501"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="554"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="597"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="934"/>
            <end position="947"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8028708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1022" mass="111520" checksum="7D24303B7315643F" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H0W2</accession>
    <name>A0A9Q1H0W2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phosphatidylinositol 3-kinase catalytic subunit type 3</fullName>
            <ecNumber evidence="1">2.7.1.137</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Phosphoinositide-3-kinase class 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_28455</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3-phosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12709"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
            <dbReference type="ChEBI" id="CHEBI:58088"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.137"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:12710"/>
        </physiologicalReaction>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.137" evidence="1"/>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H0W2"/>
    <dbReference type="OrthoDB" id="67688at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034271">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III, type I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034272">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III, type II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016303">
        <property type="term" value="F:1-phosphatidylinositol-3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00896">
        <property type="entry name" value="PI3Kc_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1070.11:FF:000002">
        <property type="entry name" value="Phosphatidylinositol 3-kinase catalytic subunit type 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1010.10:FF:000002">
        <property type="entry name" value="Phosphatidylinositol 3-kinase catalytic subunit type 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057756">
        <property type="entry name" value="PI3-kinase_type3/VPS34_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015433">
        <property type="entry name" value="PI3/4_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048:SF7">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3-KINASE CATALYTIC SUBUNIT TYPE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00915">
        <property type="entry name" value="PI3_4_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00916">
        <property type="entry name" value="PI3_4_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="9">
        <location>
            <begin position="110"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8029128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="508" mass="57480" checksum="D695388A914F1B06" modified="2023-09-13" version="1">MVEKDDQNSISKDSQIKEMYNTVMQRFGQHLMKGIPDYLKKHSQLKRQNVLINKLVEVMRQVAKESGNRKKKIERLQALLASTEFSKQMLSNFDPIPLPIQPEIKINGLIPEKASIFKSALLPCRLVFRTTENKDYTVIAKTGDDLRQDQLILQIISLMDKLLQQENLDLKLTPYNVLATSCKTGFVQFIESTAVADVLSNEGSIQNFFRKHSPKENAAYNISPDVMDTYVKSSAGYCVITYLLGVGDRHLDNLLLTTKGNLFHVDFGYILGRDPKPFPPPMKLSKEMLEGMGGVNSEQFQDFVTLCYTSFLHLRRHANLILNLFSLMVDANVPDIALEPDKTVKKLQDKFRLDLTDEEAVQYMKNLIDVSAGAMFAAWVEQIHKLAQGEGKGEGEGSLEQAPQMLILSTLVHPIFVRVVVLLNAANDKGYTHTVLEEVKGSIARRTDGLEDFTPRKEREAYNVRIVLTGVTTSQGMIPGERYLELMNSDAVLVYLQKGYHEMKVIRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H199</accession>
    <name>A0A9Q1H199_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_28071</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8031362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H199"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="90"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8031362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="186" mass="21259" checksum="8EFE9782DBB795A2" modified="2023-09-13" version="1">MAEVFSLIETCKNMYQNFEAAYDVFSKVVTNFREQRCLLKEEADFLMNRRVRNIEETLSTQVEGSLTGASNDHPDRNRAKDVIPRVFDLQKQLLKQIADVQRDMKEMRKLLEKEKRNEIAGGAKVPILAMASSIGGISMIKVARFPSFHVTSTTGFSNEVSELETLLDKVEKEVENEIEVELKSIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H371</accession>
    <name>A0A9Q1H371_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SCAN box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_25057</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H371"/>
    <dbReference type="OrthoDB" id="6077919at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF13">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCAN box" evidence="2">
        <location>
            <begin position="150"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="244"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="244"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="259"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="283"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8031754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="414" mass="48373" checksum="6DE4D6A998E8CDB8" modified="2023-09-13" version="1">MPTDNTFELKRLEMEMNKEIRLKEMEREREREEREMQMQKEKEEREMQMQREEREERERREEKAREHELFDVSKHVKFVPKFQEDNVEKFFNHFEKLGEQLKWPRDKWSILIQSNFTGKAQEVYSALSIEDSMDYDKVKKAILQAYELVPEAYRQKFRKYRKADTQTYVEFAYQKERHFDRWCASKKVSTFYALRQLMLVEQFKRCVNDDIKTHLEENKADKLKEVANLADQYALTHKFSKVGQTQNKGQFSRPNKKYGNSSGTIGNSGSQSGSISPTNSGKKQPDSSRQKNDQVTSNKATFKNIICGFCHKKGHTMANCFTLAKLKETETASNALTNVENSSEVVVTLRPNSKDQIKEEYLPFVSEGFISLDGNSAHPPVKIKILRDTGATQSLLFAIIRLYFHRGKCTNSGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H459</accession>
    <name>A0A9Q1H459_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Norsolorinic acid reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_24909</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the aldo/keto reductase family. Aldo/keto reductase 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H459"/>
    <dbReference type="OrthoDB" id="48988at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19081">
        <property type="entry name" value="AKR_AKR9C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000004">
        <property type="entry name" value="Oxidoreductase, aldo/keto reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050523">
        <property type="entry name" value="AKR_Detox_Biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43364:SF4">
        <property type="entry name" value="NAD(P)-LINKED OXIDOREDUCTASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43364">
        <property type="entry name" value="NADH-SPECIFIC METHYLGLYOXAL REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="3">
        <location>
            <begin position="24"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="3">
        <location>
            <begin position="358"/>
            <end position="613"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8031651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="639" mass="70908" checksum="11D3B7D7E48D7D6B" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H497</accession>
    <name>A0A9Q1H497_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_25303</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H497"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040446924" evidence="1">
        <location>
            <begin position="22"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8031935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="326" mass="37068" checksum="667942B50D0B7361" modified="2023-09-13" version="1">MRVFLCYFLLLLALFCQLTTEVEEKGLEKIASRMQHLKRELLDLDVFCKRSFRRDIRLLRETASKLDSARAHVSDNLGLYSSRAGLVGGTMMLAGLIPNPFSPVLFYYGSYLSMSSAIGHVSNVVLVEESYDLAEVAQVIQNLNKFYEVMIRVEDAYDALLKLQPEAILRGQNGRTLSKEKQQKILLEIEKLWDKKVNLEEKRKITLAETIDYTNSLIDFLGFARDAFDTVGGGSIDISQEDYHEYKSKAVGLPKQAYSVSKFVRDISHRSVKRVASSIIQYGSVQTQNIEEVLRKIQERGELIRLLRDTADDMERVCNGSPYCVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H4M6</accession>
    <name>A0A9Q1H4M6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Acid-sensing ion channel 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_23299</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H4M6"/>
    <dbReference type="OrthoDB" id="6021021at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015280">
        <property type="term" value="F:ligand-gated sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.820">
        <property type="entry name" value="Acid-sensing ion channel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.770">
        <property type="entry name" value="YojJ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001873">
        <property type="entry name" value="ENaC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11690">
        <property type="entry name" value="AMILORIDE-SENSITIVE SODIUM CHANNEL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11690:SF248">
        <property type="entry name" value="PICKPOCKET 17, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00858">
        <property type="entry name" value="ASC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01078">
        <property type="entry name" value="AMINACHANNEL"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0915">Sodium</keyword>
    <keyword evidence="3 11" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="9 11" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="4 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 11" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040412659" evidence="13">
        <location>
            <begin position="16"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="194"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ8033149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="223" mass="24991" checksum="121F4FE4437CC52C" modified="2023-09-13" version="1">MFSFNNLFNFTFGLGLHLTLNIEENEYVGSFTEGTGIRVMVHPSDILPHPEDVGLSVSPGLSTNIGIRQVEIRRLDAPHGDCTDGKGHEYLQNDTYSYSIISCQKKCLLDKMVSTCNCTTNIYDDYSGVPFCTGKTQTNLYITCRQRRPFGLPNNTTANLVRLKIYFQELNYELVEQVPANTIESVLGTTGGLLGLYIGFSVITISEIFVLIYDVFAFLLKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H6F0</accession>
    <name>A0A9Q1H6F0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_21929</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H6F0"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8034904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="109" mass="12220" checksum="ED3D94EA7E5FB9F0" modified="2023-09-13" version="1">MISSYVGVRGDVSLEILVSPCTHFRILCCLNRRYIRRRASEKAVLGVGGSSKHIESGKHLQFVCITFSNCWPKDLLREDGGHLKAALCKQDSVCRPETFPVMLGILWLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H6I9</accession>
    <name>A0A9Q1H6I9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">High mobility group protein DSP1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_18232</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H6I9"/>
    <dbReference type="OrthoDB" id="10070927at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050342">
        <property type="entry name" value="HMGB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056513">
        <property type="entry name" value="INO80F"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48112">
        <property type="entry name" value="HIGH MOBILITY GROUP PROTEIN DSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48112:SF22">
        <property type="entry name" value="MITOCHONDRIAL TRANSCRIPTION FACTOR A, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24245">
        <property type="entry name" value="INO80F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMG box" evidence="7">
        <location>
            <begin position="166"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="4">
        <location>
            <begin position="166"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="112"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="31"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="130"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="152"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8037422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="317" mass="35817" checksum="AB05E7905E4252E3" modified="2023-09-13" version="1">MEAVPSNGESAPTPKNGGKLSPEQDTDRRKCLLLKRKCDSVENNNKKLLNRIYHVRKTISRLSRERRGLMKKLDELKDSYKTLERTTPLEDGPFMYLLGFADQSGQRTHIKPAIDPRTGFPVVTKPKIPNKSSSFISSRGNPPASSRKKKSRDGMKADKEKDPNAPKKPANAFLMFCQEKRLSASQANKIENRKPEMSHADLTKQLAKQWNDLEEKQKKRYYVAYEREKAKYVEEVRQYSKDLQEGTSKVPPAASIASDHVGSALMKQLPADTALKQKFSKEPSKLFPTKTSVVGLLTKEQKAETAEDVDPYSFDGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H766</accession>
    <name>A0A9Q1H766_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_21949</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H766"/>
    <dbReference type="OrthoDB" id="10056483at2759"/>
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        <property type="component" value="Chromosome 10"/>
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        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15517">
        <property type="entry name" value="PHD_TCF19_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020847">
        <property type="entry name" value="AP_endonuclease_F1_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33395:SF22">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33395">
        <property type="entry name" value="TRANSCRIPTASE, PUTATIVE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00726">
        <property type="entry name" value="AP_NUCLEASE_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="6">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="6">
        <location>
            <begin position="52"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="125"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8034921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="760" mass="86383" checksum="EEEE4B2D9341A42E" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H7D8</accession>
    <name>A0A9Q1H7D8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Tyrosine-protein kinase Src42A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_22315</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H7D8"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004714">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050122">
        <property type="entry name" value="RTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416:SF631">
        <property type="entry name" value="SERINE_THREONINE_TYROSINE KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="44"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8035175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="402" mass="46488" checksum="98E3BF8018CC0FED" modified="2023-09-13" version="1">MADPVLVGIIVALAAVIVILVILLCRFGLRSRNSIDLKTGERQTYQRRREGRGSFRLVEESRRRLQRTVTTTMRRKREYLKESDPVYCRREVKTTDLLKSHGETEYWKGTFNNSTLGSQAFTVRGLKESTKIKDVIEFQRLAFILCSLSKHANIIETLAADTTKLPYYIYHETMEGVSLFDKLKENDENHYLKPVGRTPSMLTSQRGVKASSELEVLLKMSCEVTRGLSFLSSHKLFHPGINSKEVLLNANAVCKLYNFQTKATARRYIMYLITEEHRRLPWYAPETVCQHKYSSGSDIWSLGVLLFEIFSYAGVSYESLTSSELQTCYDDTRSYLPRPSGCTRSIYDIMTSCWHLDRRRRVTIDRVVSRLGNELHSFDKTERGKTARHRYQNIGLHNVQQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H975</accession>
    <name>A0A9Q1H975_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_18093</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H975"/>
    <dbReference type="OrthoDB" id="6142091at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="91"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8037305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="119" mass="14110" checksum="86CECBB242CB55FB" modified="2023-09-13" version="1">MPFNLDEFVASPSVEELDSLKKSEIVKVAKHYGIEFQPLMRKDEIKRYVLEYLVDESILPSTVLETAITVPTDNTFELKRLEIEMSKEVRLKEMEREREKEEREMQMQKEGRQENMNLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H9W4</accession>
    <name>A0A9Q1H9W4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_15799</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H9W4"/>
    <dbReference type="OrthoDB" id="10033767at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8038384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="177" mass="20140" checksum="AE5318D39E51D5BE" modified="2023-09-13" version="1">MMRNLWPYLAIAISARDLYQQVYEICDKSIPIPSVQWLRLQFWPSSTSTAAQKNTGRVKIKMPVSARQHRKHHPDTLSDSEITFVCEDDKHTIKIEESNVPVAAVERRRQVVVGLDQKMVIGDHDFSRVSLSPSLKGKNVQVFSTASGDEMKDMADQLKKIDEEFCSDILLDPDHTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HAF8</accession>
    <name>A0A9Q1HAF8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Mediator of RNA polymerase II transcription subunit 22</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Mediator complex subunit 22</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_16486</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HAF8"/>
    <dbReference type="OrthoDB" id="203279at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009332">
        <property type="entry name" value="Med22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12434">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12434:SF6">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06179">
        <property type="entry name" value="Med22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8038924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="147" mass="17388" checksum="6C97C3B08D0C3371" modified="2023-09-13" version="1">MRMAQSQQQQRTLPQSKETLLKTYQRRLKEDTRCMLDNFLEILRLAKVNPDGKIITRQLQGEEDQYEMNVRAANIVRAGESLQKLVADIKQFLILNDFPAANGYIDDRTQFLQAHQKEVDNRLIKLRDEVSVDLYEIEEEYYSSKYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HBR8</accession>
    <name>A0A9Q1HBR8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tyrosine-protein kinase ephrin type A/B receptor-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_14495</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HBR8"/>
    <dbReference type="OrthoDB" id="10062419at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11319:SF35">
        <property type="entry name" value="OUTER MEMBRANE PROTEIN PMPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01411">
        <property type="entry name" value="Ephrin_rec_like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="260"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="295"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="365"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="415"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="477"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="545"/>
            <end position="571"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8040259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="598" mass="69391" checksum="D183C3EF85FAFFAA" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HC03</accession>
    <name>A0A9Q1HC03_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_13815</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8039721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HC03"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8039721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="94" mass="10356" checksum="333B24AB67930883" modified="2023-09-13" version="1">MHFLQILKVVRPNPGGLDWTRPQVRVLRGSSPSQVRVQRGPSPSQVRVHKKMDSSPDSSPSPDSSPPALSLTYSSMFGETFLKPLGFPDDKPKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HCT1</accession>
    <name>A0A9Q1HCT1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_14814</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HCT1"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8040501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="106" mass="11917" checksum="9472448BE5080254" modified="2023-09-13" version="1">MVISIPNGHSLAQTVLSISNWIKSLQGVFVFIAFVSTEKVRGTILIWCDRFKRSDRSTHHRATRKRSHGREQGGAVSQNDPYLKATESNTQTEGDKKLIRSMESSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HCY2</accession>
    <name>A0A9Q1HCY2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_12401</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HCY2"/>
    <dbReference type="OrthoDB" id="10070745at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36981:SF1">
        <property type="entry name" value="P2X PURINORECEPTOR 7 INTRACELLULAR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36981">
        <property type="entry name" value="ZGC:195170"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8041549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="75" mass="8679" checksum="2EF8291B1E0301D8" modified="2023-09-13" version="1">MIPPRNLDDENREDRLQGTETWCNCGHCTVMPTQRECLCCHEVAKIDRRRKKGSEQPTFITLKQGFQSVCLDPDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HDI3</accession>
    <name>A0A9Q1HDI3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_11970</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HDI3"/>
    <dbReference type="OrthoDB" id="6145535at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00637">
        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24243">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24243:SF208">
        <property type="entry name" value="PYROKININ-1 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="34"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="68"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="113"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="153"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="183"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="238"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="289"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="9">
        <location>
            <begin position="47"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8041221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="371" mass="43021" checksum="D5A34999E2AC2CA3" modified="2023-09-13" version="1">MEKTVFETIQYPHRLAMFVTILDPIKSVTQIVHISFWIAFILCGFVTNGVFQFTLLRNVGVGNSLMNRLLMILSILDILILVNEFVYIAYPFFLDKFLQQQPTFHTPLVISCVWFYTLIEHFCSALFVGVAYERYAAVCFPLKNYASSGRYPLAKLVAMAFILSTLFTGIYAAVFYFCNITAVWAFYLLLFAPPLVPFITSETLYNRVIYVMFMKSPLGWLECKLPNNNARKIYRRRVVIVLFINTFVFFILNTLRKFFDSRIAQAFVKNDWDKSQDQQYAFIEKVWQWASYMTFCTVLNSTVNPVIYFIGSSQYRQALYQSCSCLRRCHANQHRSCPRSDDVTLNSISMNTERSVIAHGENPAGENDRLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HE05</accession>
    <name>A0A9Q1HE05_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Short-chain collagen C4-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_09252</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8046077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HE05"/>
    <dbReference type="OrthoDB" id="6127486at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.320">
        <property type="entry name" value="6-Phosphogluconate Dehydrogenase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051077">
        <property type="entry name" value="Ca-dependent_lectin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24024">
        <property type="entry name" value="PULMONARY SURFACTANT-ASSOCIATED PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24024:SF18">
        <property type="entry name" value="SHORT-CHAIN COLLAGEN C4-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040182200" description="Short-chain collagen C4-like" evidence="2">
        <location>
            <begin position="20"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8046077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="316" mass="33852" checksum="0F1C1E560EC2CFCC" modified="2023-09-13" version="1">MRAKLLVVVILICSNELMAQDEAELFKEEQTEEGKTKETAGHKGDNGYLSKDVTRTKRSTGQEDQAQIPGSFAPVQTSYFLGQCLMCPPGGPGPRGPPGQQGLPGRDGRDGRDQPLTSNSASTDQPSNGSLAGVTYVHWGKTTCPDNSQLVYAGIVGGKHFTQKGSGTNYLCLHSNPTFDRPQAGRNYGAYIYGTEFEAGSYAGLPQLSQEAEVACSVCLATSKHDVLMIPGVNSCPQGQGWREEYHGYLTAMHYTYSATEYICMDHEAEGIPRTGHDNDPSLLYVVEGVCGSSGGGLPCPPYVDGYEITCVVCSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HF08</accession>
    <name>A0A9Q1HF08_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Laccase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_11210</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the multicopper oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HF08"/>
    <dbReference type="OrthoDB" id="2121828at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13884">
        <property type="entry name" value="CuRO_2_tcLCC_insect_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000045">
        <property type="entry name" value="Laccase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001117">
        <property type="entry name" value="Cu-oxidase_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033138">
        <property type="entry name" value="Cu_oxidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF394">
        <property type="entry name" value="FI03373P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00394">
        <property type="entry name" value="Cu-oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00079">
        <property type="entry name" value="MULTICOPPER_OXIDASE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0186">Copper</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Plastocyanin-like" evidence="7">
        <location>
            <begin position="2"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="5">
        <location>
            <begin position="115"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="6">
        <location>
            <begin position="348"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8043904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="481" mass="54608" checksum="D9E49C5F01804824" modified="2023-09-13" version="1">MEGVTFHWHGAHQRGTPGMDGPAMITQCPIPHATTFVYNFEAEPSGTFFWHSHVASHRADGLAGALIVREPLSEDPSSPEYDEDLTRHILFIQEGNYFMQSVTYPEYLFGSGVALPDFLVVNGRGKHFPHKDYENRDIFTPRAMVYVTQGVKSRIRVISNGYTQCRLKVTIDKHRLTLIATDGYPVEPVEFDSVILSGGERFDFVLNANQEIDNYWLRVQGVGDTVCDFGAGICCTDFAEYAIVRYNGAPRQEPEDQPINSEDEIIVNSHFHDDGDNFIELSTLTSLEPYDSPPGERTTYYLSVGVNQLPGKASHIRNGMPQINRITFAYPPGPVLTQSQEMEDTLFCQIQDYKQRCEEEFCSCTQYVKARYNETVELFFYNTGANPEWCHPIHVHGYSVRVVARGRLEPQYYSPPMLEQMDREGMFPRITPAETTAVKDTICVGGSSYLILEFVADNIGWWFVHCHLDYDNMVNTSFNSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HFY0</accession>
    <name>A0A9Q1HFY0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">F-box only protein 46</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_10763</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HFY0"/>
    <dbReference type="OrthoDB" id="10052741at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039594">
        <property type="entry name" value="FBXO34/46"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16271:SF9">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16271">
        <property type="entry name" value="F-BOX ONLY PROTEIN 34/46 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="66"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8043591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="216" mass="25343" checksum="7B08FEEB53ADD5C2" modified="2023-09-13" version="1">MKSFKKALAKKWSRSDDRLEERKQRLSGLKLGSLRFQLLCHPLVSYDISELEECESDSEEEILESVTPFSHVPTYVIAKIFRLLGTKELAALKCTCKDFHWLINQFDITGTDSQWMTGKEYRSDPCMYCGKIQDPRGDVSLCQRHPKAFYKYSVINRQYWTCCNAVKETTEGCQIGLHNNRWATSEFQPNFICKPWKSQSWYITANTERWCDKEHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HGY1</accession>
    <name>A0A9Q1HGY1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Tripartite motif-containing protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_07328</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRIM/RBCC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HGY1"/>
    <dbReference type="OrthoDB" id="6105938at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017868">
        <property type="entry name" value="Filamin/ABP280_repeat-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001298">
        <property type="entry name" value="Filamin/ABP280_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047153">
        <property type="entry name" value="TRIM45/56/19-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25462">
        <property type="entry name" value="BONUS, ISOFORM C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25462:SF296">
        <property type="entry name" value="MEIOTIC P26, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00630">
        <property type="entry name" value="Filamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00557">
        <property type="entry name" value="IG_FLMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101898">
        <property type="entry name" value="NHL repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50194">
        <property type="entry name" value="FILAMIN_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="9">
        <location>
            <begin position="103"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="6">
        <location>
            <begin position="275"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="19"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="57"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8044548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="616" mass="67550" checksum="E2E0BF122B910D36" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HH35</accession>
    <name>A0A9Q1HH35_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">E3 ubiquitin-protein ligase</fullName>
            <ecNumber evidence="11">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_08604</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the UPL family. K-HECT subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="11"/>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="412600at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070534">
        <property type="term" value="P:protein K63-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21062">
        <property type="entry name" value="BTHB_HectD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000051">
        <property type="entry name" value="E3 ubiquitin-protein ligase HECTD1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000014">
        <property type="entry name" value="E3 ubiquitin-protein ligase HECTD1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1750.10:FF:000021">
        <property type="entry name" value="E3 ubiquitin-protein ligase HECTD1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000033">
        <property type="entry name" value="E3 ubiquitin-protein ligase HECTD1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2410.10:FF:000007">
        <property type="entry name" value="Putative E3 ubiquitin-protein ligase HECTD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041200">
        <property type="entry name" value="FKBP3_BTHB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045322">
        <property type="entry name" value="HECTD1/TRIP12-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012919">
        <property type="entry name" value="SUN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057948">
        <property type="entry name" value="TPR_TRIP12_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45670:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE HECTD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45670">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13857">
        <property type="entry name" value="Ank_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18410">
        <property type="entry name" value="BTHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07738">
        <property type="entry name" value="Sad1_UNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25579">
        <property type="entry name" value="TPR_TRIP12_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 10 11" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="repeat" description="ANK" evidence="9">
        <location>
            <begin position="395"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="9">
        <location>
            <begin position="426"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="HECT" evidence="13">
        <location>
            <begin position="2030"/>
            <end position="2488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="248"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="449"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1347"/>
            <end position="1417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1436"/>
            <end position="1456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1546"/>
            <end position="1575"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1612"/>
            <end position="1653"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1672"/>
            <end position="1709"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="454"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1347"/>
            <end position="1364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1373"/>
            <end position="1398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1403"/>
            <end position="1414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1555"/>
            <end position="1575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="1612"/>
            <end position="1646"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="10">
        <location>
            <position position="2457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU369009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ8045570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="2488" mass="276850" checksum="55EBFC28658E49CF" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HIX6</accession>
    <name>A0A9Q1HIX6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phospholipid scramblase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_00858</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">May mediate accelerated ATP-independent bidirectional transbilayer migration of phospholipids upon binding calcium ions that results in a loss of phospholipid asymmetry in the plasma membrane.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the phospholipid scramblase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HIX6"/>
    <dbReference type="OrthoDB" id="191150at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017128">
        <property type="term" value="F:phospholipid scramblase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005552">
        <property type="entry name" value="Scramblase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23248:SF9">
        <property type="entry name" value="PHOSPHOLIPID SCRAMBLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23248">
        <property type="entry name" value="PHOSPHOLIPID SCRAMBLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03803">
        <property type="entry name" value="Scramblase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0564">Palmitate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU363116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8048509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="76" mass="8511" checksum="ED7E8DFEDB8A4BA6" modified="2023-09-13" version="1">MSYDESRQIGKISKQWAGIVKELATNADNFGITFPLDLDVKVKATLLGALFLIDFMFFENSTQEPNNSSIPLGSYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HJI4</accession>
    <name>A0A9Q1HJI4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mitochondrial inner membrane protease subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">IMP2-like protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HOLleu_06264</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Nanhai2018</strain>
            <tissue evidence="15">Muscle</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Heterodimer of 2 subunits, IMMPL1 and IMMPL2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S26 family. IMP2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HJI4"/>
    <dbReference type="OrthoDB" id="9996127at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042720">
        <property type="term" value="C:mitochondrial inner membrane peptidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006627">
        <property type="term" value="P:protein processing involved in protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06530">
        <property type="entry name" value="S26_SPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.109.10:FF:000005">
        <property type="entry name" value="Mitochondrial inner membrane protease subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.109.10">
        <property type="entry name" value="Umud Fragment, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037730">
        <property type="entry name" value="IMP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036286">
        <property type="entry name" value="LexA/Signal_pep-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000223">
        <property type="entry name" value="Pept_S26A_signal_pept_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019533">
        <property type="entry name" value="Peptidase_S26"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46041">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE PROTEASE SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46041:SF2">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE PROTEASE SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10502">
        <property type="entry name" value="Peptidase_S26"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00727">
        <property type="entry name" value="LEADERPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51306">
        <property type="entry name" value="LexA/Signal peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="5 15" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Peptidase S26" evidence="14">
        <location>
            <begin position="35"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S26" evidence="14">
        <location>
            <begin position="107"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="active site" evidence="13">
        <location>
            <position position="41"/>
        </location>
    </feature>
    <feature type="active site" evidence="13">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR600223-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF10502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ8047293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="187" mass="21467" checksum="8B8E5AAB987347D9" modified="2023-09-13" version="1">MTSNTSRSLAKLFCGTYFIGVAGSVTFFDKVGYLATVDGRSMQPVFNPKKSKWRDIIFCNRWFVQRHKIEKGDIVSLVSPNHPNEVLVKRVIALEGETIRTLNYKMRHVTIPKGHCWIEGDNHEQSLDSNFFGPVTVGLIHSKATHIIWPLHRIQRLKSELPAGRTVLDRRKVDELLDKEELLEEVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HK64</accession>
    <name>A0A9Q1HK64_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Zinc finger matrin-type protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_00316</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HK64"/>
    <dbReference type="OrthoDB" id="6436350at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052644">
        <property type="entry name" value="ZMAT3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46786">
        <property type="entry name" value="ZINC FINGER MATRIN-TYPE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46786:SF1">
        <property type="entry name" value="ZINC FINGER MATRIN-TYPE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="310"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="236"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="376"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="445"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="664"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="246"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="707"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="753"/>
            <end position="767"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8048136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="785" mass="85812" checksum="F8DD70BED5EF6CB3" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HKC7</accession>
    <name>A0A9Q1HKC7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Solute carrier family 35 member G1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_03653</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HKC7"/>
    <dbReference type="OrthoDB" id="306876at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22911">
        <property type="entry name" value="ACYL-MALONYL CONDENSING ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22911:SF6">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER G1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="73"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="129"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="164"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="194"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="224"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="281"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="6">
        <location>
            <begin position="12"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8050444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="303" mass="33352" checksum="2071E0B9F7DE8AE0" modified="2023-09-13" version="1">MDTYFNIIRKYSGVIFSVSYGIILATQLSLGKLLVASHVHPTQIVFFRCLIHLFCIAPSTFQHETVAFTAYDTTVYFLCALTGSFSWIFTAYTFLYLEVGDSTGIELGATVVFTAIMAHVCLSEYVDKFDVIILVVDVAGIILISKPAFLFGGMEHYEQNHLKAVLMVSVSALFSSLWPMFVKNLSKMEKLYFMLLNFLHGAIGVPLTLLCSLFLQPWFTSSSAIIWTLTLLFSVVCLIQTAVQASALHLTDAKTVAVSSTISTVLTYVIQLTAFGAAFDWVVISGAFLIAVTVICSQCRTGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HKR4</accession>
    <name>A0A9Q1HKR4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Immunoglobulin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_01280</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HKR4"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312">
        <property type="entry name" value="NACHT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312:SF2">
        <property type="entry name" value="NUCLEOTIDE-BINDING OLIGOMERIZATION DOMAIN-CONTAINING PROTEIN 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040196580" description="Immunoglobulin domain-containing protein" evidence="2">
        <location>
            <begin position="24"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="479"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="domain" description="Immunoglobulin" evidence="3">
        <location>
            <begin position="27"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="Immunoglobulin" evidence="3">
        <location>
            <begin position="245"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8048811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="699" mass="78411" checksum="B8BE3F53688C866D" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HLR6</accession>
    <name>A0A9Q1HLR6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GIY-YIG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_04226</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HLR6"/>
    <dbReference type="OrthoDB" id="10025388at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10442">
        <property type="entry name" value="GIY-YIG_PLEs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1440.10">
        <property type="entry name" value="GIY-YIG endonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000305">
        <property type="entry name" value="GIY-YIG_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035901">
        <property type="entry name" value="GIY-YIG_endonuc_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01541">
        <property type="entry name" value="GIY-YIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GIY-YIG" evidence="1">
        <location>
            <begin position="85"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8050865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="191" mass="21892" checksum="1E1C37406FD87891" modified="2023-09-13" version="1">MSFDDPALSGIFSDPLLLALRQPPNLRRILTSSKLLTLDYASKGNAHCNKKRCQICKHITTEERINIPNINFPIIPPNLNCDSCNVVYIFLCNLCKDKLYVGETGTSFRLRFNNHKQSIRDNTLGFPVAEHFNLPHHSIENLKVILIANNFICAEQRKRTELVWIMKLKSHITGLNKDLGLLNQYTFQQTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HM69</accession>
    <name>A0A9Q1HM69_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_04209</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HM69"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8050851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="53" mass="6094" checksum="50B44676CF12F1DF" modified="2023-09-13" version="1">MLKKEEENPPQTANLVLMEQTRKTMQATEHVFVQTSTHAKIDLDHVQFVSKKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0Y9Q0</accession>
    <name>A0A9Q0Y9Q0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Disintegrin and metalloproteinase domain-containing protein 10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_44346</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000813">
        <property type="protein sequence ID" value="KAJ8017935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0Y9Q0"/>
    <dbReference type="OrthoDB" id="2149267at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006509">
        <property type="term" value="P:membrane protein ectodomain proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051489">
        <property type="entry name" value="ADAM_Metalloproteinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001590">
        <property type="entry name" value="Peptidase_M12B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45702">
        <property type="entry name" value="ADAM10/ADAM17 METALLOPEPTIDASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45702:SF2">
        <property type="entry name" value="KUZBANIAN, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13688">
        <property type="entry name" value="Reprolysin_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50215">
        <property type="entry name" value="ADAM_MEPRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M12B" evidence="2">
        <location>
            <begin position="127"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="282"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="281"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="285"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="291"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8017935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="361" mass="40484" checksum="3B9509BF9E5DD92C" modified="2023-09-13" version="1">MVLVTADGTYPKHYLDEIHFYKGQIADIPESFVHGSIKDGLFQGRIHSKNEWYSVEPAHRYVNDTTKFHSVIYNDKDVEFPEHPSGNCGITEEIQKKMTEKLMASTDPFSDTEDNHRQKRQNQIDKQTCALHIITDHVFFARYMDSNEILRQMGEHVQAATEVFSGHDFSPYSNINFAIERITLYNNDDIGSISYPFDGDYIGAEKYLDIASSIADNQRYCLVFAFTNRDFANGVLGLAWIPNAANRAGGVCADSFNAGIVTTNNYGENVSPVVSHLTFAHELGHSFGSRHDPDGSRECTPGGEDGNYLMFAHASAGDLPNNNEFSSCSVTNITRVLNSIGTIRNNCFDGKTSYQYYSRLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YB54</accession>
    <name>A0A9Q0YB54_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Homeobox protein Mohawk</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_41134</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TALE/IRO homeobox family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8019507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YB54"/>
    <dbReference type="OrthoDB" id="21495at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048468">
        <property type="term" value="P:cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007517">
        <property type="term" value="P:muscle organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008422">
        <property type="entry name" value="KN_HD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11211:SF3">
        <property type="entry name" value="HOMEOBOX PROTEIN MOHAWK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11211">
        <property type="entry name" value="IROQUOIS-CLASS HOMEODOMAIN PROTEIN IRX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05920">
        <property type="entry name" value="Homeobox_KN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 6 9" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="8">
        <location>
            <begin position="59"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="6">
        <location>
            <begin position="61"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="20"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="144"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="253"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="144"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="177"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="262"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="279"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8019507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="335" mass="38462" checksum="2171CEFDB80DCB63" modified="2023-09-13" version="1">MLTDSDSSWYLSPLPLRIDGPMLNEPQDEGSTTNSIEHENIRRHGLRTRHNRKTTTVVTNRTKMKRQTLFDMTKPLKAWLYEHKHHPYPTKTEKLLLALGSKMTLIQVNNWFANARRRLKNTVRRPGLTWSRRIRLYNKHASENAEQLSVTSEESTLSDLKYDHDEPDNQSERSRHSSGQSNQTTSSVPSTSASLERRDNMADFHNPVHKYKHSILQRYLNDSILHSSSFPVPNMSLLPEGQNGLQYRRSKVNSFSGSVSSHDYEDIKSPERREETQTTDRASPSPSQADTIKLAEEDKEDCNSNQESETHWLEIDAALALTNLARARRGQALCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YBL6</accession>
    <name>A0A9Q0YBL6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_42465</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000077">
        <property type="protein sequence ID" value="KAJ8019135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YBL6"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8019135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="107" mass="12228" checksum="E6C5C7584924898E" modified="2023-09-13" version="1">MPLLAKLSRKTTHLPVRLRKGVYAKLLVERLKRNIIPTPKEYRNKTSHFVKESSTPTMPGSYQGKDTEKVVSFYERDEISRCMPGRRDATKNGTTEDAKSFLDGLSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YC57</accession>
    <name>A0A9Q0YC57_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_43116</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000210">
        <property type="protein sequence ID" value="KAJ8018731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YC57"/>
    <dbReference type="OrthoDB" id="6271650at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004748">
        <property type="term" value="F:ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009263">
        <property type="term" value="P:deoxyribonucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000358">
        <property type="entry name" value="RNR_small_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23409:SF21">
        <property type="entry name" value="CAPSID PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23409">
        <property type="entry name" value="RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE SMALL CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="110"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8018731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="532" mass="60084" checksum="7EBB819358B69851" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YC70</accession>
    <name>A0A9Q0YC70_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_43004</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000183">
        <property type="protein sequence ID" value="KAJ8018804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YC70"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8018804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="106" mass="12246" checksum="68C642F5E814DA49" modified="2023-09-13" version="1">MHWVHFSLSACIDSGMAVDHCHRWHVPLFSFRVVLHLLVMHTSLHIRIISVIITVLEWHIFGDLNTFLQEVSSLKGDLLCAVYAIIRRGKYSFFYLCAVASYTLDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YCI3</accession>
    <name>A0A9Q0YCI3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Integrase core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_42757</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000130">
        <property type="protein sequence ID" value="KAJ8018956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YCI3"/>
    <dbReference type="OrthoDB" id="10045093at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058913">
        <property type="entry name" value="Integrase_dom_put"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46791:SF13">
        <property type="entry name" value="CLR5 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46791">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24764">
        <property type="entry name" value="rva_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase core" evidence="1">
        <location>
            <begin position="138"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF24764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8018956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="385" mass="44648" checksum="5A5D555F0CC31CF1" modified="2023-09-13" version="1">MEVLDKLSSLGIKMGLKGEELQRFVEKERDMVREELNLQEFSAGAVVKSVQSKRHLKRIFGRLGLFRRQNHDDVVDVALRIGEEIESSGRLHGYRWMHNKLKNNGFNTSKETVLELLRILDPEGVQIRRRRRLRRRRYNGHGPNFILHLDSYDKLRPYGVCINGCIGGYSRRVLWLEAYNTSSDPSLIAGYFMKKVQELGGTLRVVRADMGTENGTDRDMQIFLRWDDADQLAAERSFMYDRSVLDGSVWLFKRRGLFDGGYIDKALIQFCLLRPIQKELDSFVTQWNSHRIASSRMSNGPFGQPIVMYSLPHLNNTHDFLHDVPIDEVNVCIDECHFKSQIPCCSLIFNISHIIMSENNLGIPYDPEEATYLYQFLRAEIENLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YF69</accession>
    <name>A0A9Q0YF69_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_39978</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YF69"/>
    <dbReference type="OrthoDB" id="10633671at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8020396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="140" mass="16672" checksum="99015D9058A64E7D" modified="2023-09-13" version="1">MGYHADKSYLSDGWYFQHYLTRTTEERDMPHCRSQYGARIFFEDPRGADEAHGFLYMRVGNDDFMTEKIFMSNTGNNAYEPGRSYYVTGMGNFFADPLTKLEFSWEYDGDWYNPISWDLFRNPDVYVSRIEVDSLDIRQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YG99</accession>
    <name>A0A9Q0YG99_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Tripartite motif-containing protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_39606</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8022192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YG99"/>
    <dbReference type="OrthoDB" id="252722at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006513">
        <property type="term" value="P:protein monoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047153">
        <property type="entry name" value="TRIM45/56/19-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25462">
        <property type="entry name" value="BONUS, ISOFORM C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25462:SF229">
        <property type="entry name" value="TRANSCRIPTION INTERMEDIARY FACTOR 1-BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="12"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="7">
        <location>
            <begin position="93"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="146"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8022192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="245" mass="27525" checksum="6805BCEBE5852F4D" modified="2023-09-13" version="1">MAEGGHSLNVSCSICFEDYKTPKVLTCGHTFCCQCVSKMVGRNSYLQCPTCRKDISLPESGKVEDFTTNFALLSLILDQKKIKKGDGHLLKADPGFECPQHKNPTTFYCKSCKKSVCAECLLQDHKPDKHTIATTSDMAVIFSGELQKHLQQLKVYENEAKSLGKKISEGLHCYEEDKKTVQDQVNIALERKKEMLEKSAEKLINELHKKAKGHTGAYTDLQAKHSTLCDLSSKIHKYVMTYTII</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YIM2</accession>
    <name>A0A9Q0YIM2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Neuronal acetylcholine receptor subunit alpha-6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_38308</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8023197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YIM2"/>
    <dbReference type="OrthoDB" id="5975154at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005230">
        <property type="term" value="F:extracellular ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18989">
        <property type="entry name" value="LGIC_ECD_cation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040402888" evidence="6">
        <location>
            <begin position="22"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="255"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="285"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="317"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="384"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="7">
        <location>
            <begin position="35"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="8">
        <location>
            <begin position="261"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8023197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="413" mass="47002" checksum="5646C9D27A52001D" modified="2023-09-13" version="1">MFSTGRFIRVLLCASFWGIEALLLCECANDAVVRDLVQNLTRGYGFPYVRPVKNSSSPVAVGFRFTPIISTLNDIEQELKMRGMMALTWEDHRLSWKPQEYDGIQIINMPLKEGVKQLIWQPELVLNESFGSGYQSLHPETMTVSVQFNGTVLWYSLATIFSFCKVTVESYPFDIQTCGLTFTTWMFNKNEQYFYPIYDLITDEKYKKYEVKHGKWELSIKSVTKGEASYDCHTCAHTKQSFILYVLQLRRSHNFLHFVTILLPCLVMSLMTLLVMWLPRNSDSAAAGFGLTCVLTLFVFLTFLFSQLPSTGQPILGAYISWLIVEGVLITIAAVYDARILQKAKNSLKEEFSGVCANKVSERSRLFPKEKSSCLPRGTNLRKVVLFVKLSTTFIFAIIFLLLIGNKIGEGLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YIP2</accession>
    <name>A0A9Q0YIP2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Arylamine N-acetyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_40746</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YIP2"/>
    <dbReference type="OrthoDB" id="10260017at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2140.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053710">
        <property type="entry name" value="Arylamine_NAT_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8020997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="424" mass="48582" checksum="EF75F9C5D2C56C18" modified="2023-09-13" version="1">MLKQGATTRIEEYLRQRIFSNCLALTGKTQANMLSNVLKASRLFSTASTQGICCSGIQTSAGHAQQSKNKSAIVSSPPCPLSGNVIQRHRKPAKVYHTCQRFGSSYVKQTRETEDIPYPLTDDEALYFLENILKISQPLKLLKKDRVEFLNVFLFQMNTYIPFNNIQFVGRVFDKDYSLTFSEGKMAMFAQQGSTCLTINLFTKAILDSLGYKAFHIGANNPGDITFDSHVSIIICDLTFQGSLHLVDPGMTRPFYEAIPLDFSEESKIYQFNYLKCKLFKGDHGVVHLCTFIPEGQTATSPTVIESKGMSWKIQISYRPHLKRSREYFYQKMNKILGFPSIMPDVFRSIGIITRRDGRSFHLMYQDDLFSLRYMHKDPSQCEVFVLTKEEFINFMEKEFPQFPRSLVEKAVQNKILFNNLYGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YIZ2</accession>
    <name>A0A9Q0YIZ2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Histone H2B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HOLleu_35759</name>
        <name evidence="17" type="ORF">HOLleu_35761</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Nanhai2018</strain>
            <tissue evidence="17">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 13">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the histone H2B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YIZ2"/>
    <dbReference type="SMR" id="A0A9Q0YIZ2"/>
    <dbReference type="OrthoDB" id="1733721at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22910">
        <property type="entry name" value="HFD_H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000016">
        <property type="entry name" value="Histone H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000558">
        <property type="entry name" value="Histone_H2B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055333">
        <property type="entry name" value="HISTONE_H2B_site"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23428">
        <property type="entry name" value="HISTONE H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00621">
        <property type="entry name" value="HISTONEH2B"/>
    </dbReference>
    <dbReference type="SMART" id="SM00427">
        <property type="entry name" value="H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00357">
        <property type="entry name" value="HISTONE_H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0158">Chromosome</keyword>
    <keyword evidence="9 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="12 13" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="11 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="15">
        <location>
            <begin position="24"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="9"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ8023346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ8023348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="123" mass="13649" checksum="35503AFB1400FE6E" modified="2023-09-13" version="1">MAPKAPGKGAKKAAKSKAPRAPGDRKRKRTRRESYSIYIYKVMKQVHPDTGISSRAMSIMNSFVNDIFERIAAEASRLAHYNRKSTITSREVQTAVRLLLPGELAKHAVSEGTKAVTKYTTSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YJ78</accession>
    <name>A0A9Q0YJ78_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_35976</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YJ78"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8023513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="118" mass="13046" checksum="57429F6F482628B1" modified="2023-09-13" version="1">MWMWYGERKISVIFGGGQRSSGITRGQTLKTFFIPYRKMCYGKWKNPIVFGGGQRSSGVTGGQTLKTLLTRYLKIGSLEEFHTWSVDAVCKRKNSIVFGGGQKSSGVTGGQNLKTLLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YKN9</accession>
    <name>A0A9Q0YKN9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MYND-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_37219</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YKN9"/>
    <dbReference type="OrthoDB" id="9922773at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01753">
        <property type="entry name" value="zf-MYND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144232">
        <property type="entry name" value="HIT/MYND zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01360">
        <property type="entry name" value="ZF_MYND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50865">
        <property type="entry name" value="ZF_MYND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MYND-type" evidence="5">
        <location>
            <begin position="56"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8022349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="622" mass="72589" checksum="FE22E49B14F9FBFC" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YLM9</accession>
    <name>A0A9Q0YLM9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Inactive 2'-5' oligoadenylate synthetase 1C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_34748</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 2-5A synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YLM9"/>
    <dbReference type="OrthoDB" id="10057353at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001730">
        <property type="term" value="F:2'-5'-oligoadenylate synthetase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.20">
        <property type="entry name" value="2'-5'-oligoadenylate synthetase 1, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018952">
        <property type="entry name" value="2-5-oligoAdlate_synth_1_dom2/C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11258">
        <property type="entry name" value="2-5 OLIGOADENYLATE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11258:SF11">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10421">
        <property type="entry name" value="OAS1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81631">
        <property type="entry name" value="PAP/OAS1 substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50152">
        <property type="entry name" value="25A_SYNTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="346"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="2'-5'-oligoadenylate synthetase 1" evidence="3">
        <location>
            <begin position="159"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8024748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="368" mass="42753" checksum="2F635E74172AA88B" modified="2023-09-13" version="1">MPFSGKLKAFAQDLAPDHALISSAKETINEISQAIQAHFKGRGVVDRVIPFGSLPKKTSIHNKMDIDVIVYINNQHPPFYDFIEELFELLPCKCQCRSKCDCGKNEFGIKFCFDDFKLDLLPAVNFLPSRPRHRYSKGQSVRVQHQRALDYVKTVPEDKRHYYSTSLSGVSVLFEKERDSFSHSLSRLAKYWSFKVPVDNIRSRSSIMEYMGAYAADRLNGNQDIVEGFRRFLQLFESSDSLKIFWTNFYRKEDVPKRKWTERPLLLDPCNPYNNFLDTKYNASKIAQMESYAQETLTRLNAAEMNYNAGMQVNFADIFDQDHVVLFPDENEVDCECCSMKRVCTWMKIFCLFVCLVVIIFCYNVFGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YM44</accession>
    <name>A0A9Q0YM44_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Translational activator of cytochrome c oxidase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_35001</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TACO1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YM44"/>
    <dbReference type="OrthoDB" id="2017544at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.200:FF:000002">
        <property type="entry name" value="Probable transcriptional regulatory protein CLM62_37755"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.980">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00693">
        <property type="entry name" value="Transcrip_reg_TACO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017856">
        <property type="entry name" value="Integrase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048300">
        <property type="entry name" value="TACO1_YebC-like_2nd/3rd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049083">
        <property type="entry name" value="TACO1_YebC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002876">
        <property type="entry name" value="Transcrip_reg_TACO1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026564">
        <property type="entry name" value="Transcrip_reg_TACO1-like_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029072">
        <property type="entry name" value="YebC-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12532">
        <property type="entry name" value="TRANSLATIONAL ACTIVATOR OF CYTOCHROME C OXIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12532:SF0">
        <property type="entry name" value="TRANSLATIONAL ACTIVATOR OF CYTOCHROME C OXIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20772">
        <property type="entry name" value="TACO1_YebC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01709">
        <property type="entry name" value="Transcrip_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75625">
        <property type="entry name" value="YebC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TACO1/YebC-like N-terminal" evidence="4">
        <location>
            <begin position="97"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="TACO1/YebC-like second and third" evidence="3">
        <location>
            <begin position="175"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF20772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8024939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="339" mass="37959" checksum="D3BC417C14BD9871" modified="2023-09-13" version="1">MATLAKTVFFQKTLQHLKQPHQYLQKWLRVNRCQIATYPWPVLNSPHCSRLRGSLSHGYVRTTEGGQVSKHSAVILTCRFHQKCCLHTSSFTLAGHNKWSKVKHIKGPKDSEKSLMTAKVISQMRVAVREGGHNPESNPELSHLINVAKSKGVPKSTVENFLKNATKSKHKVNQAICEVQGPKGAAVVVEYMTDNQKRTRDELLKIVRKHGCSVAAQGTLLYAFKRKGILEVNEPEGQSPLTFDMAMDLAIELGAEDVLEEEENGKPMYKLICDKADFRSVREALHKTPYQCGMASLDYIAETTTELNASELEKIGSLISDLEMHPDVTRISHNVTEES</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YME3</accession>
    <name>A0A9Q0YME3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Teneurin-3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_35197</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8025089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="5986940at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.180.10.10">
        <property type="entry name" value="RHS repeat-associated core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011635">
        <property type="entry name" value="CARDB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055353">
        <property type="entry name" value="DUF7619"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022385">
        <property type="entry name" value="Rhs_assc_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050708">
        <property type="entry name" value="T6SS_VgrG/RHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056823">
        <property type="entry name" value="TEN-like_YD-shell"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03696">
        <property type="entry name" value="Rhs_assc_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32305">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32305:SF15">
        <property type="entry name" value="PROTEIN RHSA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07705">
        <property type="entry name" value="CARDB"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24595">
        <property type="entry name" value="DUF7619"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25023">
        <property type="entry name" value="TEN_YD-shell"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EGF-like" evidence="3">
        <location>
            <begin position="3723"/>
            <end position="3757"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="3747"/>
            <end position="3756"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8025089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="4010" mass="441837" checksum="A6AFAC424354AD23" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YMK9</accession>
    <name>A0A9Q0YMK9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_38890</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YMK9"/>
    <dbReference type="OrthoDB" id="10143739at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8021631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="111" mass="12617" checksum="66735093A1118DFF" modified="2023-09-13" version="1">MDLIPEIPASPTNSTDSEEEKITCGQRTPPPRKDKILLKEEVYQKVRQHADTLGQLQAATIALTEEIKSLDVHLEKRTIPQHLRVYRNPPRLPKSLNCSQDFSDSWDSAVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YNL9</accession>
    <name>A0A9Q0YNL9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ferritin</fullName>
            <ecNumber evidence="6">1.16.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_35980</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>4 Fe(2+) + O2 + 4 H(+) = 4 Fe(3+) + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:11148"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="1.16.3.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the ferritin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.16.3.1" evidence="6"/>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YNL9"/>
    <dbReference type="OrthoDB" id="186462at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008199">
        <property type="term" value="F:ferric iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004322">
        <property type="term" value="F:ferroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01056">
        <property type="entry name" value="Euk_Ferritin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1260.10:FF:000002">
        <property type="entry name" value="Ferritin, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001519">
        <property type="entry name" value="Ferritin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009040">
        <property type="entry name" value="Ferritin-like_diiron"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014034">
        <property type="entry name" value="Ferritin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008331">
        <property type="entry name" value="Ferritin_DPS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11431">
        <property type="entry name" value="FERRITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11431:SF75">
        <property type="entry name" value="FERRITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00210">
        <property type="entry name" value="Ferritin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00540">
        <property type="entry name" value="FERRITIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00204">
        <property type="entry name" value="FERRITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50905">
        <property type="entry name" value="FERRITIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="2 6" id="KW-0409">Iron storage</keyword>
    <keyword evidence="3 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ferritin-like diiron" evidence="7">
        <location>
            <begin position="60"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR601519-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8023517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="226" mass="26247" checksum="6E49F7EAAA6AE536" modified="2023-09-13" version="1">MSKSTLYCCSRLMKTVTPSDETRQTCLPQPPSSLLIVRFFSSLLKKHQRTSKMAVSQCRQNYHEESEAGVNRQINMELYASYVYMSMAYYFDRDDVALPGAHKYFKKASEEEREHAERLMKFQNQRGGRVKLQDIKRPERDEWSSLLSAFTIALELEKKVNESLLNLHSLADSHKDAQMCDFIETHYLTEQVEAIKEIGDHITNLKRVGTGLGEYIYDKESLKEDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YNX1</accession>
    <name>A0A9Q0YNX1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_34807</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YNX1"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="CDD" id="cd00117">
        <property type="entry name" value="TFP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="180"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8024795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="211" mass="23445" checksum="D41A1A9F6D48C712" modified="2023-09-13" version="1">MGGLLQNVTLGLKQILTTVLFALCVLPSLMDLTLAHTASMVTALDAFTKDLTTVFPFENILPNNPDQYKPITCYKCGAYRHRDGDDFEKKYKECKDLDTSRAETEDCPTFLAGQFTNATCRVTTNTITSQVTSFSRGCSFLLPCSELKECYDSTKKYSGVCRSCCKETLCNSSPPTHSTWSILALSLLIKFCFSLPILCFVMYSCRTDESG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YPC8</accession>
    <name>A0A9Q0YPC8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Deleted in malignant brain tumors 1 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_33797</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8026063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YPC8"/>
    <dbReference type="OrthoDB" id="536948at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000001">
        <property type="entry name" value="Lysyl oxidase 4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000006">
        <property type="entry name" value="neurotrypsin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000016">
        <property type="entry name" value="Scavenger receptor cysteine-rich protein type 12"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000007">
        <property type="entry name" value="Soluble scavenger receptor cysteine-rich domain-containing protein SSC5D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000009">
        <property type="entry name" value="WC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48071:SF18">
        <property type="entry name" value="DELETED IN MALIGNANT BRAIN TUMORS 1 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48071">
        <property type="entry name" value="SRCR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00530">
        <property type="entry name" value="SRCR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00258">
        <property type="entry name" value="SPERACTRCPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00420">
        <property type="entry name" value="SRCR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="12">
        <location>
            <begin position="39"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="12">
        <location>
            <begin position="140"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="12">
        <location>
            <begin position="242"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="12">
        <location>
            <begin position="353"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="12">
        <location>
            <begin position="465"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="12">
        <location>
            <begin position="572"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="12">
        <location>
            <begin position="676"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="12">
        <location>
            <begin position="783"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="105"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="206"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="312"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="423"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="531"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="638"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="746"/>
            <end position="756"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8026063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="860" mass="93853" checksum="4C7EE068B4F1DE5F" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YQ84</accession>
    <name>A0A9Q0YQ84_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">P2X purinoceptor 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_31319</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YQ84"/>
    <dbReference type="OrthoDB" id="10066060at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046815">
        <property type="entry name" value="P2RX7_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36981:SF3">
        <property type="entry name" value="UBIQUITIN-LIKE PROTEASE FAMILY PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36981">
        <property type="entry name" value="ZGC:195170"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20478">
        <property type="entry name" value="P2RX7_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="P2X purinoreceptor 7 intracellular" evidence="1">
        <location>
            <begin position="53"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF20478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8026488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="187" mass="21724" checksum="9D34AB324A90E1F9" modified="2023-09-13" version="1">MRGAQVAQQQEERRRMRRERIRAMVDNLDLEGMRNFLRILVERQPALFLEIWEQQPQAAGPALPEQPHWCNCSRCQEMPQLLEEVCCRGGMDSCLSMEPVEMDALVLDPGVLDLARLTLNDMFGMRENNEPNHTMRHVAYRQFTVWQYGRLGRGNRHVIPSCVVTRIRATYPSPNGQYRGYVPGRLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YQV8</accession>
    <name>A0A9Q0YQV8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Solute carrier family 49 member A3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_33516</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YQV8"/>
    <dbReference type="OrthoDB" id="422206at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17399">
        <property type="entry name" value="MFS_MFSD7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049680">
        <property type="entry name" value="FLVCR1-2_SLC49-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10924">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10924:SF6">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 49 MEMBER A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="70"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="107"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="168"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="238"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="288"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="326"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="352"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="380"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="483"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8025845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="537" mass="58949" checksum="F52F80311A16D6EB" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YSA0</accession>
    <name>A0A9Q0YSA0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_31515</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YSA0"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8026625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="95" mass="11021" checksum="48CF8B22B4381369" modified="2023-09-13" version="1">MDRAYCTNVPCVNCLHRTYTNRLTNMAACYSLVCKSLDLLKWLSIDKLCEKLVIFSKECKLRSNSSNRMENCDIVLPFILIFCLKCCRFIVKLGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YTU9</accession>
    <name>A0A9Q0YTU9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Metabotropic glutamate receptor 8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_32107</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YTU9"/>
    <dbReference type="OrthoDB" id="425344at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000337">
        <property type="entry name" value="GPCR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050726">
        <property type="entry name" value="mGluR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24060">
        <property type="entry name" value="METABOTROPIC GLUTAMATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00248">
        <property type="entry name" value="GPCRMGR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040433967" evidence="7">
        <location>
            <begin position="27"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="Receptor ligand binding region" evidence="8">
        <location>
            <begin position="65"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8027072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="194" mass="21833" checksum="6A69C1230C3E8073" modified="2023-09-13" version="1">MKRVEDYQFIGLLFAALLLFLCNVEANLNYSSIGQLNIGGLFPLHFYDEPKRTCGSLREIGSLRRMEAMVYAINKINQNTSVLNNISLGYEIYDTCSYPAETLKQSLNFIPPYRKASSCECDNPGKNNSLSNEIIGVVGPQRSSSSVQSTILLSLYNIPQVSYLSTSDELSNDERYPYFLRTVPPDRFQVSTVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1B9U3</accession>
    <name>A0A9Q1B9U3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_43100</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000207">
        <property type="protein sequence ID" value="KAJ8018740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1B9U3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8018740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="85" mass="9576" checksum="FCAFBB56D646F865" modified="2023-09-13" version="1">MKREMERGEGCYKLGKCNELGIELELGKCNKLELGKCNKLELGIELELGKCNKLELGIELELGKCNKLELGIELELGKCNKLAWH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BBK4</accession>
    <name>A0A9Q1BBK4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_42507</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000083">
        <property type="protein sequence ID" value="KAJ8019099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BBK4"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8019099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="139" mass="14317" checksum="33B6CC404CCA9B3F" modified="2023-09-13" version="1">MVEEPRSPSPDEVAHQVEQDFLAAKQCEGVSGTPEAGPPSSSSSTPGEAGLLTVENDLQLSSDSETDSAMSDAGGPASEPPPKEPPSGEGWFDLCEGVETESKLKRSASGEESVEGIHPSGPPLPSPCKNKKARSETDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BBL8</accession>
    <name>A0A9Q1BBL8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NXPE family member 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_41088</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NXPE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8019473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BBL8"/>
    <dbReference type="OrthoDB" id="5950832at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057106">
        <property type="entry name" value="NXPE4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026845">
        <property type="entry name" value="NXPH/NXPE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16165">
        <property type="entry name" value="NXPE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16165:SF5">
        <property type="entry name" value="NXPE FAMILY MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06312">
        <property type="entry name" value="Neurexophilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24536">
        <property type="entry name" value="NXPE4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NXPE C-terminal" evidence="2">
        <location>
            <begin position="290"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8019473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="514" mass="58402" checksum="C53859106C910BC5" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BC52</accession>
    <name>A0A9Q1BC52_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_42480</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000079">
        <property type="protein sequence ID" value="KAJ8019127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BC52"/>
    <dbReference type="OrthoDB" id="10063152at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051055">
        <property type="entry name" value="PIF1_helicase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47642">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47642:SF5">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8019127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="112" mass="13363" checksum="EC9BD8E3926B29E8" modified="2023-09-13" version="1">MDIQYVLKSYACVMYIVPYISKGQRGMSYLMQRATREARDGNHDIKQRVRHIGNKFLNHVELSAQRVVYLVLQMSLRKARQQFVFFNISLPEERTVLLKPFECYPRATRRLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BG66</accession>
    <name>A0A9Q1BG66_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Integrin beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">HOLleu_34347</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="23">Nanhai2018</strain>
            <tissue evidence="23">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 18">Cell membrane</location>
            <topology evidence="1 18">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 18">Belongs to the integrin beta chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BG66"/>
    <dbReference type="OrthoDB" id="5956021at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008305">
        <property type="term" value="C:integrin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033627">
        <property type="term" value="P:cell adhesion mediated by integrin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000036">
        <property type="entry name" value="Integrin beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000075">
        <property type="entry name" value="Integrin beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000002">
        <property type="entry name" value="Integrin beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1240.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.100">
        <property type="entry name" value="Cytochrome c1, transmembrane anchor, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1510">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013111">
        <property type="entry name" value="EGF_extracell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040622">
        <property type="entry name" value="EGF_integrin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057073">
        <property type="entry name" value="EGF_integrin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033760">
        <property type="entry name" value="Integrin_beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015812">
        <property type="entry name" value="Integrin_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014836">
        <property type="entry name" value="Integrin_bsu_cyt_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012896">
        <property type="entry name" value="Integrin_bsu_tail"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036349">
        <property type="entry name" value="Integrin_bsu_tail_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002369">
        <property type="entry name" value="Integrin_bsu_VWA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032695">
        <property type="entry name" value="Integrin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057243">
        <property type="entry name" value="Integrin_I-EGF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10082">
        <property type="entry name" value="INTEGRIN BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10082:SF60">
        <property type="entry name" value="INTEGRIN BETA-PS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07974">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23105">
        <property type="entry name" value="EGF_integrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18372">
        <property type="entry name" value="I-EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08725">
        <property type="entry name" value="Integrin_b_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07965">
        <property type="entry name" value="Integrin_B_tail"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00362">
        <property type="entry name" value="Integrin_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17205">
        <property type="entry name" value="PSI_integrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002512">
        <property type="entry name" value="Integrin_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01186">
        <property type="entry name" value="INTEGRINB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00187">
        <property type="entry name" value="INB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01241">
        <property type="entry name" value="Integrin_b_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01242">
        <property type="entry name" value="Integrin_B_tail"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69687">
        <property type="entry name" value="Integrin beta tail domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69179">
        <property type="entry name" value="Integrin domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103575">
        <property type="entry name" value="Plexin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00243">
        <property type="entry name" value="I_EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="11 18" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15 17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13 18 23" id="KW-0401">Integrin</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="5 18 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="777"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin beta subunit VWA" evidence="20">
        <location>
            <begin position="85"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin beta subunit tail" evidence="22">
        <location>
            <begin position="689"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin beta subunit cytoplasmic" evidence="21">
        <location>
            <begin position="800"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="86"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="89"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="98"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="250"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="301"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="474"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="503"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="518"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="527"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="532"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="543"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="573"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="575"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="580"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="598"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="619"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="621"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="631"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="642"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="663"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="665"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="670"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="689"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="695"/>
            <end position="767"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR002512-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM00187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="SMART" id="SM01241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="SMART" id="SM01242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KAJ8024438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="846" mass="93706" checksum="10A3BFA618DA2616" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BGZ0</accession>
    <name>A0A9Q1BGZ0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">B box-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_36962</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8024272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BGZ0"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047153">
        <property type="entry name" value="TRIM45/56/19-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25462">
        <property type="entry name" value="BONUS, ISOFORM C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25462:SF296">
        <property type="entry name" value="MEIOTIC P26, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="B box-type" evidence="3">
        <location>
            <begin position="113"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="167"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8024272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="595" mass="67395" checksum="EFD1B7BC864A4BEC" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BHL4</accession>
    <name>A0A9Q1BHL4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_34104</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8026298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BHL4"/>
    <dbReference type="OrthoDB" id="10009629at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05325">
        <property type="entry name" value="carb_red_sniffer_like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051468">
        <property type="entry name" value="Fungal_SecMetab_SDRs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43544:SF12">
        <property type="entry name" value="NAD(P)-BINDING ROSSMANN-FOLD SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43544">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE/REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8026298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="261" mass="28644" checksum="05A8594D32D9B591" modified="2023-09-13" version="1">MTSVVLVQGASRGLGLQFCRNLLQRPSTSVVATCRAPEDATHLQQLEASNKDRLEILKLDVTQEDAIRDAAKLVKEKFKNLDLVINCAGMLHPSGKGETSLRDVSTQGLNTTFLTNAFGPLLVAKYFSPMLAAGSGSFGIQSENAKERHSGILVNMSARVGSITDNGFGGWYSYRMSKSALNMATKNLSIELGRPRNKIICVALHPGSVDTDLTRPYQRNIPKDKLFSTTYSVECLMKIISNLRIEDTGKYLAWNGKEIPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BI79</accession>
    <name>A0A9Q1BI79_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TIP41-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_31396</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TIP41 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BI79"/>
    <dbReference type="OrthoDB" id="10253878at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031929">
        <property type="term" value="P:TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051330">
        <property type="entry name" value="Phosphatase_reg/MetRdx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007303">
        <property type="entry name" value="TIP41-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21021">
        <property type="entry name" value="GAF/PUTATIVE CYTOSKELETAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21021:SF16">
        <property type="entry name" value="TIP41-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04176">
        <property type="entry name" value="TIP41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8026539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="278" mass="31759" checksum="EE5EBB4A95578569" modified="2023-09-13" version="1">MSVNVHKMSSLKVEKKQKIEEFSFGPWKITTIKDHILQSSALESIEKELAIPQLPEMLFGNNLLKVEHIAGFGIAFNAVDALKKVDTKQESLQVAAAESWQKARSDCEFLKKVIKPFDWTFTTDYNGTITSSGPEITVTQTTDCIDIEKLKVREKIHFYEDITLFEDELADNGIATLGVKIRVMQSSFFLLMRFFLRVDGVLIRMNDTRVYHEAGSNFMLREYSSKEKKISELDLQPYELTDPVSVEKYLDVKLQTFEKLEFPPEALEAEKTAAAKNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BIJ3</accession>
    <name>A0A9Q1BIJ3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pre-mRNA-splicing factor SYF2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_32172</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in pre-mRNA splicing.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">May be part of a spliceosome complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the SYF2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BIJ3"/>
    <dbReference type="OrthoDB" id="199717at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013260">
        <property type="entry name" value="mRNA_splic_SYF2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13264">
        <property type="entry name" value="GCIP-INTERACTING PROTEIN P29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13264:SF5">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR SYF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08231">
        <property type="entry name" value="SYF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5 7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0747">Spliceosome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="123"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="167"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="14"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="46"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="98"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8027122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="260" mass="30406" checksum="72604AAB4E8DBD39" modified="2023-09-13" version="1">MASLDTAKPSDAGSSSMRHGGKSASSSSSSSSSPSTSGVNKTAAQKKFERMQKLRELQMKRNEARKLNHAEVVEEDRRNKLPENFEAKKRKGEWILQEEEKKKAAEARGEDYDRIKVLDVSAEDAEKWERKKKKKNPDQGFSDYEQASFRQYQRLTKQMKPDMEEYQRNKEKMGQDMYPSVDSLMQGTHKDSEEGINRMVADLEKQIEKRSKYSRRRAFNEEDDIDYINERNMRFNKKIERFYGKYTTEIKQNLERGTAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BJL2</accession>
    <name>A0A9Q1BJL2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Serine/threonine-protein kinase PLK</fullName>
            <ecNumber evidence="13">2.7.11.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Polo-like kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">HOLleu_29858</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">Nanhai2018</strain>
            <tissue evidence="19">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.21"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.21"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
            <location evidence="1">Centriole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.21" evidence="13"/>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8027804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BJL2"/>
    <dbReference type="OrthoDB" id="10004143at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13114">
        <property type="entry name" value="POLO_box_Plk4_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13116">
        <property type="entry name" value="POLO_box_Plk4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000221">
        <property type="entry name" value="Putative serine/threonine-protein kinase PLK4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000576">
        <property type="entry name" value="Serine/threonine-protein kinase PLK4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.930:FF:000001">
        <property type="entry name" value="Serine/threonine-protein kinase PLK4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1120.120:FF:000001">
        <property type="entry name" value="serine/threonine-protein kinase PLK4 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1120.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1120.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047108">
        <property type="entry name" value="Plk4-like_POLO_box_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000959">
        <property type="entry name" value="POLO_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033699">
        <property type="entry name" value="POLO_box_Plk4_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033698">
        <property type="entry name" value="POLO_box_Plk4_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033696">
        <property type="entry name" value="POLO_box_Plk4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046437">
        <property type="entry name" value="Ser_Thr-PK_POLO_box_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345:SF91">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE PLK4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18190">
        <property type="entry name" value="Plk4_PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18409">
        <property type="entry name" value="Plk4_PB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82615">
        <property type="entry name" value="Polo-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51984">
        <property type="entry name" value="CPB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51985">
        <property type="entry name" value="CPB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50078">
        <property type="entry name" value="POLO_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6 13 19" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="12"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="Cryptic POLO box 1 (CPB1)" evidence="17">
        <location>
            <begin position="631"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="domain" description="Cryptic POLO box 2 (CPB2)" evidence="18">
        <location>
            <begin position="746"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="domain" description="POLO box" evidence="16">
        <location>
            <begin position="946"/>
            <end position="1023"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="271"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="439"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="533"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="860"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="276"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="300"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="466"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="533"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="568"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="617"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="881"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAJ8027804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1029" mass="114111" checksum="19FA70D47D0F388C" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BKU6</accession>
    <name>A0A9Q1BKU6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_30528</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BKU6"/>
    <dbReference type="OrthoDB" id="6502630at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8028325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="81" mass="9078" checksum="490C3D372A787F09" modified="2023-09-13" version="1">MRYGKRKNPIIFDGGQRSSGVTGGRMLKTLLTQYLKMRYGKRKNPIVCGRGQRSSGVTGGQTLKTLLTRYLKIGSLDKFHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BLU3</accession>
    <name>A0A9Q1BLU3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_28244</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8028973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BLU3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="71"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8028973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="96" mass="10838" checksum="8052A43B385B713A" modified="2023-09-13" version="1">MRRLSRAGKVRAYQRKSQDHRPPAVQDGFVAGLEDNYPHRQAFQWLVGSCEFIGVLTMPKCYGLAFQNENLILYVIALVFWCVCVWGGGDLCSRAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BMK6</accession>
    <name>A0A9Q1BMK6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Sushi, von Willebrand factor type A, EGF and pentraxin domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_28684</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Virion</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BMK6"/>
    <dbReference type="OrthoDB" id="6127264at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051503">
        <property type="entry name" value="ComplSys_Reg/VirEntry_Med"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45785">
        <property type="entry name" value="COMPLEMENT FACTOR H-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45785:SF2">
        <property type="entry name" value="COMPLEMENT FACTOR H-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0732">Signal</keyword>
    <keyword evidence="2 5" id="KW-0768">Sushi</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040186714" evidence="6">
        <location>
            <begin position="24"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="7">
        <location>
            <begin position="145"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8029319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="215" mass="23564" checksum="7879B94D3852B941" modified="2023-09-13" version="1">MSLKVLHLTAILFLTGNVGFSAAECNGVPSACDPEDILRGRSSSKACYNDEETLKYSCNGILHGPPENICKNGEWMIPAPIDCEDFTCDIPTDDSFATLDPRKSIYNAREEVKIMCVAGYKLEGPRSAICEPQGWNPLTTPKCFKTCKSPEVQHGRAQIGSPMIKGDTATVVCDDGYVIHVGQSHWITCGDNGEWEHIPQCVKDERSPESPLNDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BMZ4</accession>
    <name>A0A9Q1BMZ4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_29044</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BMZ4"/>
    <dbReference type="OrthoDB" id="8963689at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF13">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8029605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="172" mass="20390" checksum="232E5DF36AD21F70" modified="2023-09-13" version="1">MRKDEIKRYVLEYLVDESILPSTVLETAITVPTDNTFDLKRLEMEMNKEVRLKETEREREKKQKEKEEREMQMQREEKAREHEFTLKQLELGVIKGSDAKIGLDTGGFDVSKHVKFVPKFQEDNVEKFFNHFEKLGEQLKWPRDKWSILIQSNFTGKAQEVYSALNRGQHGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BN78</accession>
    <name>A0A9Q1BN78_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_28959</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0449 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BN78"/>
    <dbReference type="OrthoDB" id="6129359at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028227">
        <property type="entry name" value="UPF0449"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34766">
        <property type="entry name" value="UPF0449 PROTEIN C19ORF25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34766:SF1">
        <property type="entry name" value="UPF0449 PROTEIN C19ORF25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15136">
        <property type="entry name" value="UPF0449"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="55"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8029539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="134" mass="15160" checksum="26731076144D92C8" modified="2023-09-13" version="1">MPKLLEKRPEPPVLNEILEDLNNSSPDDIVFNFFATNAKSSEIADFGADQTKGVTKNNAELTNDRKKRSAGKEMKTGEESAEVEECYKRVKTLIESYNYLKTSQTTLTEKCSELEEMGQKVQQQITELKGHLTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BNB5</accession>
    <name>A0A9Q1BNB5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Dynein heavy chain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_29109</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BNB5"/>
    <dbReference type="OrthoDB" id="7699643at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8029661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="99" mass="11698" checksum="822F9C52A0D7CBF3" modified="2023-09-13" version="1">MHGLKYQVHGMKYQVHVMKHQVDGMKYQVHGIKYQVHGMKCQVHVMKYQVHGMKYQVDGMKYQVHFMKNLVYGVKYEVHGMKYPVHLYGDKASPVSRSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BNR7</accession>
    <name>A0A9Q1BNR7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_29486</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BNR7"/>
    <dbReference type="OrthoDB" id="10034966at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912:SF36">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912">
        <property type="entry name" value="IP13529P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8029948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="835" mass="95446" checksum="5F2D50842B0410DE" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BNX5</accession>
    <name>A0A9Q1BNX5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_29574</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8030009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BNX5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8030009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="214" mass="24075" checksum="A517A1C9EB994F74" modified="2023-09-13" version="1">MAFSFDHKTGSSYTLTIFLESPMRAGSCYLGLEETTGKIFRLRDPHGRFVWPGFPLFTVGKRYIFFKADTKYVSPFCEDICVLPSSTRCVETEGDDSITEPIFEKLLLRAHPSVEGVFNFQVKGCKYTNEGCKCPAFGIWEYSGGGIDIYEQSDDYRDQQKRISLGGYDFPYKGSFPCAKIERRETDTPLLVVVGLSEPFAGSEDNILILEGVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BPX3</accession>
    <name>A0A9Q1BPX3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">NKAP-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_26873</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NKAP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BPX3"/>
    <dbReference type="OrthoDB" id="273141at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040466">
        <property type="entry name" value="NKAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009269">
        <property type="entry name" value="NKAP_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13087">
        <property type="entry name" value="NF-KAPPA B ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13087:SF0">
        <property type="entry name" value="NFKB ACTIVATING PROTEIN LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06047">
        <property type="entry name" value="Nkap_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NF-kappa-B-activating protein C-terminal" evidence="3">
        <location>
            <begin position="341"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="46"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="77"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="117"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="142"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="194"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="256"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF06047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8030440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="445" mass="51458" checksum="F2380A3220FA3A1B" modified="2023-09-13" version="1">MKIKWMKMEKGTVSGGIDCQRNFSSSTSDNRNILKKYIVPPLSIMVRGRTESDSQSRSRSPVSRNRSRSRSPVDRHSKVHKSKHRSTRRSRTPSPKRHKVRRRSRSRDRKRSRSRSKSSNSSSSSRSDSPRRGEKRQRKRSTSRERLRPERQHNGSGHQRDWDMKYRDDIRANGAQRRNFQSDGGRFSEAYQEQQEKFYEIRRSERSKVASIGVPEVWAASPIKPEEDSDENSEKEEADKSKASSAESTDSEEERRKKKKKKKAKRREEKKKKKSRKKKSSKKSKKRKKEASSSSESESEESSDENIEKEWVEACAAKRDEDSVIGPLPITSLSGTSGLMDYGKALLPGEGAAMAAYVNEGKRIPRRGEIGLTSEEIESYEEMGYVMSGSRHRRMEAVRLRKENQIYSADEKRALATFSKEERVKRESVILSDFRELVRKKTKDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BSK0</accession>
    <name>A0A9Q1BSK0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_25229</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BSK0"/>
    <dbReference type="OrthoDB" id="10063284at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8031880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="150" mass="16181" checksum="965826F15BA056EF" modified="2023-09-13" version="1">MLGARRAFGAPAPRRYDEPGSSGTAPELSELPQSPADKFRTRTFLVTVDTNAALQKRLDAYKDIAAKFGFLRDLKDLPDDQVIKNADSLQKAYPTDLEDSLPDELVHFSSFLNTEFATKALSATVPTAISAANSTTQTTTEEADGVAVIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BU21</accession>
    <name>A0A9Q1BU21_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HAT C-terminal dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_23340</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BU21"/>
    <dbReference type="OrthoDB" id="6159421at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008906">
        <property type="entry name" value="HATC_C_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46880:SF5">
        <property type="entry name" value="DUF4371 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46880">
        <property type="entry name" value="RAS-ASSOCIATING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05699">
        <property type="entry name" value="Dimer_Tnp_hAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HAT C-terminal dimerisation" evidence="1">
        <location>
            <begin position="188"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8033183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="250" mass="28110" checksum="F10F32CE23B8E223" modified="2023-09-13" version="1">MEKAFASLYFLVQRRIAHTTNSEPLLDLLSFLGVNVKEQIRVAKNATYTSDKTVQEMLYVISEVLEDDTLKELASSDYFALMFNESSDCTNLEQLALHAQYLNSAGDQVKFLKIINVLQPEIDAIARTARNGDGDPNEKGIISLNAEVITKGVKEYIKQEWVSLKNYITQVSEMSDTDLIQRLCTRQALQAVYPNFSQLAKILRVIPTNTADPETTFSQMKLVKTKVRNRTVEQTLDAIFQGCDRGSSLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BY93</accession>
    <name>A0A9Q1BY93_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Solute carrier family 35 member G1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_21805</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BY93"/>
    <dbReference type="OrthoDB" id="306876at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22911">
        <property type="entry name" value="ACYL-MALONYL CONDENSING ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22911:SF6">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER G1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="82"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="142"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="172"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="197"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="231"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="263"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="295"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="323"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="349"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="81"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="233"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="371"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="18"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="375"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="395"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8034805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="406" mass="44053" checksum="1179C7362D9424C2" modified="2023-09-13" version="1">MEEAHDNPCFILDEPSLGETNTASKPRNANGDIDNSYPYNGSVNCTESHTQPKAENSTKVLSNERPLPVFSRGRKLLYRRRGVILALGGAVFFSAENMFMDVLADSLNIFQIGSVMSLVVIACTFTFIVIKSIDFPKTNWQYISLVISGLFLASGLPLALESLKFIEVGDSITIIYTMPLFSGIFSWIILREPLRIIDCLFAVLSFGGVVLIARPPFLFGTDGDKLVGHNPLLGAVYALASAASFSLLVVTARKQSIMGIHPVTILFFNAIVVLVTNAIVCTILSEWAFPNPAEWGFLLGFGFSDFLAQTFIYMAVVVEVAMFVSIISTFEVILTFLWQFLFLHIAPDWISVVGAALILASCVGLATKKDPPTADDNNNDQQSSGSAENHAPLPSISASVSASTSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BYN7</accession>
    <name>A0A9Q1BYN7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NACHT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_22154</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BYN7"/>
    <dbReference type="OrthoDB" id="120976at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312">
        <property type="entry name" value="NACHT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312:SF2">
        <property type="entry name" value="NUCLEOTIDE-BINDING OLIGOMERIZATION DOMAIN-CONTAINING PROTEIN 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="257"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="NACHT" evidence="2">
        <location>
            <begin position="403"/>
            <end position="578"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8035063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="992" mass="113379" checksum="0FD6EBF25FF1BDE0" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BZ20</accession>
    <name>A0A9Q1BZ20_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Thyrotroph embryonic factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_19233</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bZIP family. NFIL3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8035522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BZ20"/>
    <dbReference type="OrthoDB" id="6022300at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14695">
        <property type="entry name" value="bZIP_HLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000025">
        <property type="entry name" value="nuclear factor interleukin-3-regulated protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040223">
        <property type="entry name" value="PAR_bZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11988:SF27">
        <property type="entry name" value="GH27708P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11988">
        <property type="entry name" value="THYROTROPH EMBRYONIC FACTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07716">
        <property type="entry name" value="bZIP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="9">
        <location>
            <begin position="261"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="49"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="177"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="286"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="49"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="177"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="213"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8035522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="339" mass="37213" checksum="67464993947FF1EA" modified="2023-09-13" version="1">MSEQEDIFDDSIESLEYSELKDGHQVIPPISSFSRGSDVVEEGFESAASNYYGPNTASRNDSSRSIDTTGPSFPAEVQSRVTSVSSNDNVITAAHQILGNVANFVASSSSSSASSASSIYASVTTGTQSHEKAMVELDKHRNFLNNIQQQQQAQQSQPVLPVAISVDANAFMNSSLPTAGTVAPPNTQLPPSLSLHSRPAEAGLPTRSAASHVDSSNHPSPMNINVDTVVQQGEMQVDDTDNRRQGKKARAKRTVPTEEKDDRYFERRKRNNQAAKRSRDARKVREEHISRRADFLERENEILKAQLSTLREEANSLRLLLSQRPVIPTMITSQGHQIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C0Y8</accession>
    <name>A0A9Q1C0Y8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_20303</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C0Y8"/>
    <dbReference type="OrthoDB" id="10040207at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027886">
        <property type="entry name" value="SPMIP4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31393">
        <property type="entry name" value="C5ORF31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31393:SF2">
        <property type="entry name" value="CHROMOSOME 7 OPEN READING FRAME 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="242"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="405"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="112"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="242"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="263"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="279"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="330"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="360"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8036352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="438" mass="50545" checksum="8CEF4E2BD6E8D0FA" modified="2023-09-13" version="1">MPPRPLEGRISRLIGPNQMVRCVIKDGRIQYVPYVPKIVIEMDKVREHPNLPSETHDSFRKPAEMAEWRRLQTLQHPETQLEKLKLRQATVEPLTLKLSGDTRKKAYDSEPKNYLKEMAEKHKQELKMLTENARWKEAELREHHHDLTLLKRKVDYTSPKLQPPVYYDTLHKIYTAKAPFYYDGDNPYEYDFVLPWDYHKTSAVLDQYKKEGEFLEMVRSGKFPSNAGEKYQFAGYPGVNPFVNQSNGTKQTENKPAEYEGTPSAQNLPSQSTYTHSYSPDKVREVDPKIDMSSLAKSLPVRFGDSGYDTGNSNLESDGLKHPPEANKILDNSNPNSTSASSKPLYDPAYRNPHSNPVVDSRHHNPDGTGEFRFKWTPDYPYGNRPQTSLLKLQNSFTKSDARKSFHDTFPETNPDLRDNIGSGKKHEFHGQNAYYWH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C169</accession>
    <name>A0A9Q1C169_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Integrase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_20160</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C169"/>
    <dbReference type="OrthoDB" id="10068084at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon gypsy-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF15">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase zinc-binding" evidence="2">
        <location>
            <begin position="355"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="218"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="410"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8036244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="437" mass="50288" checksum="B060FB94B5DE4E22" modified="2023-09-13" version="1">MSEPEETAEPIGRHTAAQVRREFRIFGQIGTSEQKDRVSFTSLNHQLDTGLRKCYTEQEVVDATIRAINPSLSLRRYLEGRPDVTFKKLRRLLRTHYQEKSATELYHELCSLSQQPKETAQNFMFRALDLRQKVLQASKEVEAPQQHDRDLVQNMFLHAISTGLLSTTVRNDLKPFLDNPKVSDEVLLEKVNASASVEVERGRKLGSKSKVTSNLICDGTEQPDHTQAPKMAVTPSAPPKLEKARDASGVIDARTNYVKSEIVPTVRRLSRKLTPPPSPYLKTIEPHELVAAQQNDVYIGKVLEYKLQDRRPFVKENSQKPKEVSRLLQEWKKLIVDKDGLLKRETRGRKQIVLPRKYHMLVFKELHEEMGHVGVERTISLVRDRFYWPYMARELEHYVTKQCKCIKDKKPTKPARAPMSHMQSTEPMDCVNRLSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C1Q1</accession>
    <name>A0A9Q1C1Q1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">RNA exonuclease 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_17388</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the REXO1/REXO3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8036757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C1Q1"/>
    <dbReference type="OrthoDB" id="3996471at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010629">
        <property type="term" value="P:negative regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06145">
        <property type="entry name" value="REX1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000031">
        <property type="entry name" value="RNA exonuclease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034922">
        <property type="entry name" value="REX1-like_exo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047021">
        <property type="entry name" value="REXO1/3/4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Ribonucl_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12801:SF82">
        <property type="entry name" value="RNA EXONUCLEASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12801">
        <property type="entry name" value="RNA EXONUCLEASE REXO1 / RECO3 FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exonuclease" evidence="8">
        <location>
            <begin position="197"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8036757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="471" mass="53197" checksum="E9E8C607E412B78A" modified="2023-09-13" version="1">MKNNKKLKKETVATLKQTPKRTSPKIFLNCSRKHSSNSKEQQRCKCKKTYEKVPVEDVRNLLHLSLLGDQVSQNLRWCTVKHSSRIAKVLLLIIHGLNSSCYRKNKDCFKKLCKAVSLETKVAFNSSRDIASVLFSNVPESEKPAIVSIKPDDSSNTGRLTYLVSKKKMLQMNYPKNDDEGIVSTNYANDLLTDSSPLFALDCEMCKTEDGLELTKVCLIDETGSSVLCTLVKPRNPIVNYLTLYSGITCEDLSAVETRLEDVQQAVIHLLPPDAILVGQSIENDLWALRMYHPFCIDTSDMFTQSGHRVKLADLASFYLSRNIQNDEMGHDPYEDALAALDLCKLKLRMGSNLPKYSRRNLISGDESQKLELSKSNNKVETISYTSLFESLKNSEKSCAVVDVRRILRRFENVAVDQIECINDSETVKKTLGAIRRHEFVCAQFHGLHTLLQGNYLSVDNLRVSLEIMQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C2F8</accession>
    <name>A0A9Q1C2F8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">P2X purinoceptor 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_17972</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C2F8"/>
    <dbReference type="OrthoDB" id="494673at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046815">
        <property type="entry name" value="P2RX7_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36981:SF3">
        <property type="entry name" value="UBIQUITIN-LIKE PROTEASE FAMILY PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36981">
        <property type="entry name" value="ZGC:195170"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20478">
        <property type="entry name" value="P2RX7_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="104"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="P2X purinoreceptor 7 intracellular" evidence="3">
        <location>
            <begin position="244"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="247"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="247"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="269"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF20478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8037210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="413" mass="47141" checksum="31461B446FC199B8" modified="2023-09-13" version="1">MEASGSFSCVIRDVTPDVRLKVITNSTELNIYFQKESIITTDGVSNVTLQSNFTTESGLSKCNGEFMVTCKAIDRGPPSIQIGSTARLGILIACEKPLSIEEKVWLAAITLFPFLWVFTLVVVCYVNKKNIFRVCRHERDYSPYNPEKIRQSLLNSTRRMSLTIRPRRVEDIDGERNDKSIAYVYFIDDRSCYAVSAKSPHKSIKLVLEKKKSRTNEQAASELERTSAMKQEDIELSVLETIQDSSVHDSLVQDSSVQDSSTPEKRDIQQQSSVTDNSMMDSVTELDRKASTSSNETLDDSLEWCRCGKCQELPEKHKVCCRRTYGECVTDDPLFYQLVTDRNTLQAAMIQWNDDQVASRFEAMAYKEYIYWQMGVTQNYSKVIIPLCVVKKVQSRYPKDSYGHLQKFLSLKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C2N9</accession>
    <name>A0A9Q1C2N9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">DNA-(Apurinic or apyrimidinic site) lyase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_17785</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AP endonuclease 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C2N9"/>
    <dbReference type="OrthoDB" id="7663182at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003906">
        <property type="term" value="F:DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00019">
        <property type="entry name" value="AP2Ec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.150:FF:000001">
        <property type="entry name" value="Probable endonuclease 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.150">
        <property type="entry name" value="Divalent-metal-dependent TIM barrel enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00152">
        <property type="entry name" value="Nfo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001719">
        <property type="entry name" value="AP_endonuc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018246">
        <property type="entry name" value="AP_endonuc_F2_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013022">
        <property type="entry name" value="Xyl_isomerase-like_TIM-brl"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00587">
        <property type="entry name" value="nfo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002199">
        <property type="entry name" value="PRK01060.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21445:SF0">
        <property type="entry name" value="APURINIC-APYRIMIDINIC ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21445">
        <property type="entry name" value="ENDONUCLEASE IV ENDODEOXYRIBONUCLEASE IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01261">
        <property type="entry name" value="AP_endonuc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00518">
        <property type="entry name" value="AP2Ec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="Xylose isomerase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00729">
        <property type="entry name" value="AP_NUCLEASE_F2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00730">
        <property type="entry name" value="AP_NUCLEASE_F2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00731">
        <property type="entry name" value="AP_NUCLEASE_F2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51432">
        <property type="entry name" value="AP_NUCLEASE_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Xylose isomerase-like TIM barrel" evidence="9">
        <location>
            <begin position="168"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="26"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="49"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="62"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8037059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="428" mass="47382" checksum="380A8D235F074F92" modified="2023-09-13" version="1">MPRPRKQATAADAVFEPRITRGQKRLAKEREDKITNGRNKQSKKSKQLCAEHNDHPEDTVNKTKRSNSKTKVIKKEQKTKVIKKENTTGVTQRTVKKGKENPVKVRKEQSSISSKSEKLVANEGIVKVKKEGAVKGEVTEMAGFLAKGGKFVGAHTSAAGGLWNAVSDAVEIKARAFALFLKSQRQWNAKPLEEDVITKFQSALKEHGFHPDFILPHGSYLLNCGSPKAETLQKSRDALLDEVQRCQRLGLNRYNFHPGSTCGEISVEKCLDLIGESINGVLEKTSGVTVVVENMSCQGNTVGGKFEELKGIIDRVNDKSRIGVCLDTCHAFAAGFDLSTADGYKKMMDDFEEIVGFEYLKGVHLNDSKGKVGCHLDRHENIGKGFIGLEGFRRVMNDPRFEQVPLILETPPGLTDAEEIDLLYAMID</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C2X6</accession>
    <name>A0A9Q1C2X6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SCAN box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_17717</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C2X6"/>
    <dbReference type="OrthoDB" id="10000497at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF13">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCAN box" evidence="2">
        <location>
            <begin position="176"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="270"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="527"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="270"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="285"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="309"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="527"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="549"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="575"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="598"/>
            <end position="610"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8037011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="623" mass="70037" checksum="9F134879F5B2CC4B" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C3D1</accession>
    <name>A0A9Q1C3D1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Pantetheinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_18560</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the carbon-nitrogen hydrolase superfamily. BTD/VNN family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C3D1"/>
    <dbReference type="OrthoDB" id="10250282at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040154">
        <property type="entry name" value="Biotinidase/VNN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043957">
        <property type="entry name" value="Vanin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10609">
        <property type="entry name" value="BIOTINIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10609:SF27">
        <property type="entry name" value="CN HYDROLASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19018">
        <property type="entry name" value="Vanin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CN hydrolase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8037675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="510" mass="56930" checksum="713ECC03E39C98D9" modified="2023-09-13" version="1">MTNSSKVPYQEDPKKAALIFIGIIKSLHWGSKIIVFPEYGITGFFLSATRDNIRPFLEVVPNATKPRVNPCMQGEYKERLVQRRLSCLSKEYSIIIVAGMASMQPCNKSDPHCPKDGRYQFNTAVAYGPDGNLLAIYHKSNLYSGETHFFDSGVKSDNNVATFHTKFGTFGLFICFDILFEFPATHFGKFKVDTAVFPTAWMNELPLLSAVEYQQAWALGMNMNLLASNQHFPLELMTGSGIYSATEGAVAYHYDMDTYKGKLIVSDLPCFPSMLVNTSTEGFENHDDNISHEALWTSAQSKMAQKSLKSEDTPHFLINNKTRKELYGDLERKSKALVMSSVKNDSINNDDTFISMVNHDPYTLKLLTKTQDTVSVSDSGLTCTLNYSLLGVWEKGDWYAFGGFSGLHTNNGEYYVQTCVLLRCASQQKSQCGAQTSEANTHFQHFAIQMLGSSKETFRTPEVLTRGVGLPPKGSSWDVNKEFTTIWSDAGGLKDPLLMAGIFGRLYSKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C3R9</accession>
    <name>A0A9Q1C3R9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Leucine-rich repeats and immunoglobulin-like domains protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_18499</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C3R9"/>
    <dbReference type="OrthoDB" id="6363818at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050541">
        <property type="entry name" value="LRR_TM_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369">
        <property type="entry name" value="ANTIGEN BSP, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369:SF210">
        <property type="entry name" value="CHAOPTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040112278" evidence="5">
        <location>
            <begin position="16"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="402"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8037629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="431" mass="49485" checksum="3682602A5D89AB6F" modified="2023-09-13" version="1">MLLFALMVVVLGVVGESLTVPSDNISRHYNCNEVCEYDDWFNRAHCEERHLYQVPFSTGCTEAVMLEIQENYIERLTVECLTGYRKVRTLDVSRNNISIIDSGTFLGVPRLRNLLASHNHLNELPNGTFKGPEYHLERIYLSHNELRYIDKHAFKNLRHVVLLYLTYNNIETLHPRVFLGMLKLRYLHLGKNKLKFIHHGAFHRLNSLESIDLSNNKLRWIPDGLFDGLGSLREVMLSGNDIVSLPVPRGHVRYELFDVANNNLNQSGNILPYLHISDTIYIDGNAFICDCDYLLVQQWYQNKTQSERQHYDIHNPIACEPSNDEDIPLSCDFTDSTSTTERNLSTTHSTLVITGHNLQSNQFIVTTEAAANVIQDKNLNTHHAANICGVNTDEIDGKILALSIYIATVVTLFLVLWIVKTFIFWLFGYYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C511</accession>
    <name>A0A9Q1C511_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_15939</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C511"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8038497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="109" mass="12694" checksum="2F34D2F208F76407" modified="2023-09-13" version="1">MFENKIKSNFTFFHQSCTSRWLLLSLASAISGHLGCIQISLFWYAPHQPMVALRLDSKWEPHKNLQSPEPPPPPQQKNPSYATASNERHYRVQVFDFSLIMINRSEVMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C553</accession>
    <name>A0A9Q1C553_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Laminin subunit beta-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HOLleu_16431</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Nanhai2018</strain>
            <tissue evidence="18">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
            <location evidence="1">Basement membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="9981301at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005604">
        <property type="term" value="C:basement membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009887">
        <property type="term" value="P:animal organ morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009888">
        <property type="term" value="P:tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00055">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000074">
        <property type="entry name" value="Laminin subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000090">
        <property type="entry name" value="laminin subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000094">
        <property type="entry name" value="Laminin subunit alpha-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000407">
        <property type="entry name" value="Laminin subunit alpha-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000188">
        <property type="entry name" value="Laminin subunit gamma 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.300.10">
        <property type="entry name" value="Tie2 ligand-binding domain superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050440">
        <property type="entry name" value="Laminin/Netrin_ECM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000034">
        <property type="entry name" value="Laminin_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013015">
        <property type="entry name" value="Laminin_IV_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008211">
        <property type="entry name" value="Laminin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002049">
        <property type="entry name" value="LE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056863">
        <property type="entry name" value="LMN_ATRN_NET-like_EGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10574:SF440">
        <property type="entry name" value="LAMININ EGF-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10574">
        <property type="entry name" value="NETRIN/LAMININ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00053">
        <property type="entry name" value="EGF_laminin"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24973">
        <property type="entry name" value="EGF_LMN_ATRN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00052">
        <property type="entry name" value="Laminin_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00055">
        <property type="entry name" value="Laminin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00011">
        <property type="entry name" value="EGFLAMININ"/>
    </dbReference>
    <dbReference type="SMART" id="SM00180">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00281">
        <property type="entry name" value="LamB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00136">
        <property type="entry name" value="LamNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01248">
        <property type="entry name" value="EGF_LAM_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50027">
        <property type="entry name" value="EGF_LAM_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51115">
        <property type="entry name" value="LAMININ_IVA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51116">
        <property type="entry name" value="LAMININ_IVB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51117">
        <property type="entry name" value="LAMININ_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0084">Basement membrane</keyword>
    <keyword evidence="7" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="8 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 12" id="KW-0424">Laminin EGF-like domain</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Laminin N-terminal" evidence="17">
        <location>
            <begin position="67"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="14">
        <location>
            <begin position="426"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="14">
        <location>
            <begin position="482"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin IV type B" evidence="16">
        <location>
            <begin position="560"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="14">
        <location>
            <begin position="792"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="14">
        <location>
            <begin position="901"/>
            <end position="947"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="14">
        <location>
            <begin position="948"/>
            <end position="998"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin IV type A" evidence="15">
        <location>
            <begin position="1026"/>
            <end position="1199"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="14">
        <location>
            <begin position="1296"/>
            <end position="1344"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="1479"/>
            <end position="1506"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="1818"/>
            <end position="1870"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="455"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="505"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="792"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="794"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="813"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="901"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="921"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="948"/>
            <end position="960"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="950"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="969"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="1319"/>
            <end position="1328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ8038876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1924" mass="210686" checksum="6FED9E2A2DFCCCD1" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C6M8</accession>
    <name>A0A9Q1C6M8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SRCR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_16620</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C6M8"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00530">
        <property type="entry name" value="SRCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SRCR" evidence="2">
        <location>
            <begin position="9"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8039034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="86" mass="9636" checksum="3447195C04DA6C86" modified="2023-09-13" version="1">MTTNGEQCVVMTSGGIDGARVVCHQLGYHNVTDSYKNVSIKEPEFVSMSIFQWHGNETSISSRKHERFQEDNCPIENNAGVTCEVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C762</accession>
    <name>A0A9Q1C762_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_14905</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C762"/>
    <dbReference type="OrthoDB" id="9904351at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039284">
        <property type="entry name" value="CCDC159/163"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34533:SF3">
        <property type="entry name" value="BICD FAMILY-LIKE CARGO ADAPTER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34533">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN CCDC163"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="20"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="277"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="91"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="22"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="50"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="320"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8040578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="419" mass="47535" checksum="8DA59B5B061244EE" modified="2023-09-13" version="1">MYSWERKFGDVVKDTEKHLSKARQTLDTTSHRNGYRSISGDSMEFHRSYPSENKPSARSFSSSHLHEIRKDSGYGSYQLYPATQDHSSGLVAILSEKIDRQGKLIEDLTRQVLTLERENEKSSLRLRSVEQEVTSLRQRMAENGVNIETEQKVDRYQWEMRAKLDDLKLQMETQNKLGVLSSIPPEQDWRYALTEELRDLKKHVERECDSLYHEIEVIRSKLVRYEVELTGQHGNSKELARQVERLKQEFETSASKVSAQLSNLSASSKDAAVDRLFSSSRNGDPSHHTKAVKPLKTLSSVDSEPALTLPKSRQRRRKSKSSEPVRNHSSHRPDRNFSNQLKYKTASELLSSSTDDFSSDSKEGLSKANTGQSETTPKDTDDSLTLSDLDLEDKSDTIQSSLHGVGALSIDSDSLDLDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C9C0</accession>
    <name>A0A9Q1C9C0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_15140</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C9C0"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8040750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="81" mass="9059" checksum="A96F76DF9B9E33B5" modified="2023-09-13" version="1">MLNARDTIIAEKRRCLYLAAALAIVEEKQKRLEEAEEQEKATPPRRKTQRKVVLAGAGAGTAAWGYPLDWWCVEFLGGEEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CAD0</accession>
    <name>A0A9Q1CAD0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcription factor 25</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_12586</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CAD0"/>
    <dbReference type="OrthoDB" id="205993at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990112">
        <property type="term" value="C:RQC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006994">
        <property type="entry name" value="TCF25/Rqc1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22684">
        <property type="entry name" value="NULP1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22684:SF0">
        <property type="entry name" value="RIBOSOME QUALITY CONTROL COMPLEX SUBUNIT TCF25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04910">
        <property type="entry name" value="Tcf25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8041696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="669" mass="76207" checksum="4E2274BB9A309FCE" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CAD3</accession>
    <name>A0A9Q1CAD3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Centromere protein T</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_13075</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CENP-T/CNN1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8042093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CAD3"/>
    <dbReference type="OrthoDB" id="10071342at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051382">
        <property type="term" value="P:kinetochore assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22920">
        <property type="entry name" value="HFD_CENP-T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028255">
        <property type="entry name" value="CENP-T"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035425">
        <property type="entry name" value="CENP-T/H4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46904">
        <property type="entry name" value="CENTROMERE PROTEIN T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46904:SF1">
        <property type="entry name" value="CENTROMERE PROTEIN T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15511">
        <property type="entry name" value="CENP-T_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CENP-T/Histone H4 histone fold" evidence="7">
        <location>
            <begin position="485"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="155"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="213"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="304"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="427"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="16"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="83"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="159"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="231"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="304"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="352"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="468"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF15511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8042093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="588" mass="64117" checksum="57F60382539C28D8" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CBE7</accession>
    <name>A0A9Q1CBE7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_12530</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CBE7"/>
    <dbReference type="OrthoDB" id="6502630at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8041648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="153" mass="16811" checksum="BB636FA1FD198451" modified="2023-09-13" version="1">MPHIERKNPIVFGGGQRSSGVTGGQTLKTLLTPYLEMHYGKRKKPIVCGGGQRSPGVTGGQTLKTLIKRYLKMRYGKRKNPIIFDGGQRSSGVTGGRRLKTLLTQYLKMRYGKRKNPIVFGGGQRSSGVTGGQTLKTLLTQYLKIGSVDKFHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CCG2</accession>
    <name>A0A9Q1CCG2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Alpha-1-inhibitor 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_13398</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8042366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CCG2"/>
    <dbReference type="OrthoDB" id="9998011at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004866">
        <property type="term" value="F:endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.130.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.690">
        <property type="entry name" value="Alpha-macroglobulin, receptor-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009048">
        <property type="entry name" value="A-macroglobulin_rcpt-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036595">
        <property type="entry name" value="A-macroglobulin_rcpt-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050473">
        <property type="entry name" value="A2M/Complement_sys"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011625">
        <property type="entry name" value="A2M_N_BRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047565">
        <property type="entry name" value="Alpha-macroglob_thiol-ester_cl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011626">
        <property type="entry name" value="Alpha-macroglobulin_TED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001599">
        <property type="entry name" value="Macroglobln_a2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019742">
        <property type="entry name" value="MacrogloblnA2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11412:SF136">
        <property type="entry name" value="CD109 ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11412">
        <property type="entry name" value="MACROGLOBULIN / COMPLEMENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00207">
        <property type="entry name" value="A2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07703">
        <property type="entry name" value="A2M_BRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07677">
        <property type="entry name" value="A2M_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07678">
        <property type="entry name" value="TED_complement"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01360">
        <property type="entry name" value="A2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01359">
        <property type="entry name" value="A2M_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01361">
        <property type="entry name" value="A2M_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01419">
        <property type="entry name" value="Thiol-ester_cl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49410">
        <property type="entry name" value="Alpha-macroglobulin receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00477">
        <property type="entry name" value="ALPHA_2_MACROGLOBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0882">Thioester bond</keyword>
    <feature type="domain" description="Alpha-2-macroglobulin bait region" evidence="4">
        <location>
            <begin position="29"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-2-macroglobulin" evidence="5">
        <location>
            <begin position="230"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-macroglobulin receptor-binding" evidence="6">
        <location>
            <begin position="870"/>
            <end position="959"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8042366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1002" mass="111694" checksum="4D9CF886CF064507" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CCG7</accession>
    <name>A0A9Q1CCG7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_13130</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8042140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CCG7"/>
    <dbReference type="OrthoDB" id="248923at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08222">
        <property type="entry name" value="STKc_Nek11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051131">
        <property type="entry name" value="NEK_Ser/Thr_kinase_NIMA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899">
        <property type="entry name" value="CAMK FAMILY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899:SF8">
        <property type="entry name" value="NIMA-RELATED KINASE 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="24"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="462"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="493"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="523"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="545"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="555"/>
            <end position="579"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8042140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="718" mass="80920" checksum="B26D9ACE50536B89" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CCZ7</accession>
    <name>A0A9Q1CCZ7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Low-density lipoprotein receptor-related protein 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_09963</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CCZ7"/>
    <dbReference type="OrthoDB" id="5804959at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050778">
        <property type="entry name" value="Cueball_EGF_LRP_Nidogen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000033">
        <property type="entry name" value="LDLR_classB_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46513">
        <property type="entry name" value="VITELLOGENIN RECEPTOR-LIKE PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00058">
        <property type="entry name" value="Ldl_recept_b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00135">
        <property type="entry name" value="LY"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63825">
        <property type="entry name" value="YWTD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51120">
        <property type="entry name" value="LDLRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040486982" evidence="4">
        <location>
            <begin position="27"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="5">
        <location>
            <begin position="27"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="3">
        <location>
            <begin position="236"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8043037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="451" mass="50550" checksum="B131C250C10C919D" modified="2023-09-13" version="1">MDSVRLSLCNVRKLILLSVLLASGKACFTTLEDAEGELAPPLYPQVDLVNLDCTWILRAEEGKVIEITFEEVILDATIDQAGNCLDRITVYDGPRQIIPPFCDDGPPWPRFPIRLISTTDTMVINLVKQREASRGFLAKYKHVSPRQELLAVINVDNPAIALFDRLSSLSSSKEIHLLHSSSPSALSFDPISEIFYFTDIQKRAIGRASLRNSTVSFIIEENVDTPLGIAVAYLTGLLYWTDAGRHDVSVSRLDGTFRKTLMTSSPDCWTPKGIVLSRDNQDIFWTCKGRIEKAKADGSERDPTFVIDNVVEPSALAIDTDGTFIYWVDTGAGKIDRVRVDGLYREQMDQSPNSFLTQLHSLVLDEFAYFFTHTSDQKVYIIERGTEEIELRFLELDPPETFGGLYYYNSDMAIQAQHACAINNGGCEGLCFPDRDDVKCVDGENPLYFKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CD73</accession>
    <name>A0A9Q1CD73_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Nicotinate-nucleotide pyrophosphorylase [carboxylating]</fullName>
            <ecNumber evidence="5">2.4.2.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Quinolinate phosphoribosyltransferase [decarboxylating]</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_09642</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the catabolism of quinolinic acid (QA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>nicotinate beta-D-ribonucleotide + CO2 + diphosphate = quinolinate + 5-phospho-alpha-D-ribose 1-diphosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:12733"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:29959"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57502"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.19"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from quinolinate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Hexamer formed by 3 homodimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NadC/ModD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.19" evidence="5"/>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8042786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CD73"/>
    <dbReference type="OrthoDB" id="10067394at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004514">
        <property type="term" value="F:nicotinate-nucleotide diphosphorylase (carboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034213">
        <property type="term" value="P:quinolinate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01572">
        <property type="entry name" value="QPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000090">
        <property type="entry name" value="Nicotinate-nucleotide pyrophosphorylase [carboxylating]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.20">
        <property type="entry name" value="Quinolinate phosphoribosyl transferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004393">
        <property type="entry name" value="NadC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027277">
        <property type="entry name" value="NadC/ModD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036068">
        <property type="entry name" value="Nicotinate_pribotase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037128">
        <property type="entry name" value="Quinolinate_PRibosylTase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002638">
        <property type="entry name" value="Quinolinate_PRibosylTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022412">
        <property type="entry name" value="Quinolinate_PRibosylTrfase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00078">
        <property type="entry name" value="nadC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32179">
        <property type="entry name" value="NICOTINATE-NUCLEOTIDE PYROPHOSPHORYLASE [CARBOXYLATING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32179:SF3">
        <property type="entry name" value="NICOTINATE-NUCLEOTIDE PYROPHOSPHORYLASE [CARBOXYLATING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01729">
        <property type="entry name" value="QRPTase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02749">
        <property type="entry name" value="QRPTase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51690">
        <property type="entry name" value="Nicotinate/Quinolinate PRTase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54675">
        <property type="entry name" value="Nicotinate/Quinolinate PRTase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Quinolinate phosphoribosyl transferase N-terminal" evidence="13">
        <location>
            <begin position="193"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="Quinolinate phosphoribosyl transferase C-terminal" evidence="12">
        <location>
            <begin position="269"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00020990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00033102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ8042786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="462" mass="51383" checksum="85AB6AF2F80BFDB5" modified="2023-09-13" version="1">MAKIVETFSFEHITLKRGSGFLVDAYIYTRKVVRKGRVMVRCQERKCPAKGYILDDQAHITNDSHNHPPPDVRAIRFRAQLKEAIAEDRNKNLSVPDVYEQVKWNFMQQAETEQQREELEATLPTRTSVERALHRRRSEILPQVSTPRQAPKKVKPQMDYFGNMIPASHLKEAVQSWLREDIPNFDFGGFVVGDKTEKAVLLCKSKGVLAGVPFFNAVFSEVGCTVQWHHEEGTTLDPVCKVATVKGKVCNILRGERTALNCITRASGIASLAKRLSSMAKQYSWHGQIAGTRKTTPGFRMVEKYALIVGGVSTHRYDLSSMIMLKDNHIWSSGSIREAVGNAKKVGGFSTKVEVECRSLQEATEAASAGADVIMLDNFQAEAAHRVADELKCLFPHLLIEVSGGITETMLPSFFGPCIDVISMSRLTQGYGVVDFSLKIQKEGVDPSNPTITVNPNDPSSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CES5</accession>
    <name>A0A9Q1CES5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1</fullName>
            <ecNumber evidence="4">4.1.3.44</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Radical S-adenosyl methionine and flavodoxin domain-containing protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">tRNA wybutosine-synthesizing protein 1 homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">tRNA-yW-synthesizing protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">HOLleu_11341</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="22">Nanhai2018</strain>
            <tissue evidence="22">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Probable component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. Catalyzes the condensation of N-methylguanine with 2 carbon atoms from pyruvate to form the tricyclic 4-demethylwyosine, an intermediate in wybutosine biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>N(1)-methylguanosine(37) in tRNA(Phe) + pyruvate + S-adenosyl-L-methionine = 4-demethylwyosine(37) in tRNA(Phe) + 5'-deoxyadenosine + L-methionine + CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:36347"/>
            <dbReference type="Rhea" id="RHEA-COMP:10164"/>
            <dbReference type="Rhea" id="RHEA-COMP:10165"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64315"/>
            <dbReference type="ChEBI" id="CHEBI:73542"/>
            <dbReference type="EC" id="4.1.3.44"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">tRNA modification; wybutosine-tRNA(Phe) biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TYW1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.44" evidence="4"/>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8044006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CES5"/>
    <dbReference type="OrthoDB" id="271553at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102521">
        <property type="term" value="F:tRNA-4-demethylwyosine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031591">
        <property type="term" value="P:wybutosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000196">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001094">
        <property type="entry name" value="Flavdoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013917">
        <property type="entry name" value="tRNA_wybutosine-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034556">
        <property type="entry name" value="tRNA_wybutosine-synthase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13930">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT TRNA 4-DEMETHYLWYOSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13930:SF0">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT TRNA 4-DEMETHYLWYOSINE SYNTHASE TYW1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00258">
        <property type="entry name" value="Flavodoxin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08608">
        <property type="entry name" value="Wyosine_form"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00369">
        <property type="entry name" value="FLAVODOXIN"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00284">
        <property type="entry name" value="tRNA_wybutosine-synthesizing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01071">
        <property type="entry name" value="tRNA_wybutosine-synthesizing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="13" id="KW-0456">Lyase</keyword>
    <keyword evidence="19" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0819">tRNA processing</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="Flavodoxin-like" evidence="20">
        <location>
            <begin position="107"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="21">
        <location>
            <begin position="381"/>
            <end position="625"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00025368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00078095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00081169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00082357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KAJ8044006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="713" mass="80738" checksum="7AE9DFADD4A5D680" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CFT3</accession>
    <name>A0A9Q1CFT3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_11095</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CFT3"/>
    <dbReference type="OrthoDB" id="8963689at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8043824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="70" mass="8330" checksum="77ECA761543955FB" modified="2023-09-13" version="1">MESSRELNFNAAFLELSRRENCLRYVPIIAATWQHFEEAAIIVIQLLCVLEVSIKERSRNYWTVYTRSFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CGE1</accession>
    <name>A0A9Q1CGE1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Neuronal acetylcholine receptor subunit alpha-7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_07243</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the ligand-gated ion channel (TC 1.A.9) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CGE1"/>
    <dbReference type="OrthoDB" id="5975154at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005230">
        <property type="term" value="F:extracellular ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18989">
        <property type="entry name" value="LGIC_ECD_cation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19051">
        <property type="entry name" value="LGIC_TM_cation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000028">
        <property type="entry name" value="AcetylCholine Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040544006" evidence="5">
        <location>
            <begin position="34"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="251"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="311"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="442"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="7">
        <location>
            <begin position="40"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="8">
        <location>
            <begin position="258"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="364"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="364"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8044481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="470" mass="54335" checksum="B0740716D01B2D20" modified="2023-09-13" version="1">MGIFRKVTRRLSVRMASTFIVSLLLIHERPANGAIKTASKRLVEDLLSGYGSPFVRPVKNTNNTVEVIFRAKPVWFADFDERSQILKFTALNQLKWKDELLTWNPDDYEGIKEINFGKEQIWFPDIAIMERLDVGPWKVNMEEPFTKVNFKGEVVYYYMMLISVYCKMDVTLFPFDIHDCELILIPYSFDAKEVSLYYSRDKEAEENLLKKNGVWFVSDYHAKEVEIHHLCCPEPYREIHFTLRVQRESGFYVLTFGVPSVLLSLTSVAVFKLHPESGEKISLCVNNVLVLIIFQQLLASNMPPTGDNTPIVAYYFIAVITTGFMSVLATTLVLGMYHHDASRPLPEWVRRLLRIKPVNRSRKSERGIFDSRNKPDTEPSRNSNKARLSHEYDGNKNSIVKVVRNPSVTYRKRVPVESLENGISDISYQGEWREASRKLDKILFWICMSILILVLLISMYFLSLGKQATD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CGI6</accession>
    <name>A0A9Q1CGI6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_11684</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8044270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CGI6"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040472036" description="Secreted protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8044270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="182" mass="20418" checksum="74F0D1DE4833665D" modified="2023-09-13" version="1">MAHKFRILISVVILWSQGVNSLERKHLTDLREFRVQGLASKVEVPKSVVEDLFKNITTDSDRLGFPLSELGSNNIFCDLYGTLFLGCGVLHDHTLSGVSCEMKERVAASYNIFHIPLSFVSGGQSGIIFLVLFTDSDKDIFPYSTNWNHVHHIDEIEEAEDGTITLRQGNDVMAIKTQVHVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CGV7</accession>
    <name>A0A9Q1CGV7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">THO complex subunit 6-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_11662</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat THOC6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8044254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CGV7"/>
    <dbReference type="OrthoDB" id="273067at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000347">
        <property type="term" value="C:THO complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000346">
        <property type="term" value="C:transcription export complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042626">
        <property type="entry name" value="THOC6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44411">
        <property type="entry name" value="THO COMPLEX SUBUNIT 6 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44411:SF1">
        <property type="entry name" value="THO COMPLEX SUBUNIT 6 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="184"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8044254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="364" mass="40455" checksum="7BFD1532B18184E5" modified="2023-09-13" version="1">MFLTCQPRGITGMPESQRFMKEQVKSRDLLLQDEQIQEELRKQHMNVFAQCYTLDGKHVATGNNFGIVAIYNMTAALSSGANENSWKPAFKFKVFDGAVYTMVSTETFLICAGWGDIKAYKWTDVLSKDPKVVWILRVPHLDNLGGNETNAMVLNSKDQTLFSGGGDGVVYCWDLESGSVKTSFTGHKDYIQALSLFHNSDQLASAAEDGSVRIWDTRTPGEAVAMMEPYKNEECSKSSNERWLGCVQVSPSDDWLVCGGGPSLALWHLRSLSPTTIFDAPKCHPYAAHFYEDTVISAGSTPHVYHWNLSGSLKTQVPCSSNTVYNIAIHLDNRDNKVMCASGSSHKTDIYTNFGYRAFSLDFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-06-18" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CGY9</accession>
    <name>A0A9Q1CGY9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Kinesin-like protein KIF16B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_08084</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000021">
        <property type="entry name" value="kinesin-like protein KIF16B isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47117">
        <property type="entry name" value="STAR-RELATED LIPID TRANSFER PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47117:SF1">
        <property type="entry name" value="STAR-RELATED LIPID TRANSFER PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
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</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
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            <taxon>Aspidochirotacea</taxon>
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    </organism>
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        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CMR0"/>
    <dbReference type="OrthoDB" id="676979at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004180">
        <property type="term" value="F:carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00185">
        <property type="entry name" value="TNFRSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001164">
        <property type="entry name" value="GH01279p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.10">
        <property type="entry name" value="Tumor Necrosis Factor Receptor, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050467">
        <property type="entry name" value="LRFN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45842:SF12">
        <property type="entry name" value="KEKKON 5, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45842">
        <property type="entry name" value="SYNAPTIC ADHESION-LIKE MOLECULE SALM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01411">
        <property type="entry name" value="Ephrin_rec_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040390024" evidence="6">
        <location>
            <begin position="23"/>
            <end position="1073"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="654"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="697"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="765"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="810"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="868"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="1001"/>
            <end position="1019"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="1025"/>
            <end position="1051"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8048217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1073" mass="123229" checksum="5D720E4C597A17EA" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CNK8</accession>
    <name>A0A9Q1CNK8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transposable element P transposase-like GTP-binding insertion domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_00177</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CNK8"/>
    <dbReference type="OrthoDB" id="10063305at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048366">
        <property type="entry name" value="TNP-like_GBD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21788">
        <property type="entry name" value="TNP-like_GBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposable element P transposase-like GTP-binding insertion" evidence="1">
        <location>
            <begin position="17"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF21788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8048023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="90" mass="10100" checksum="CD3E7710A9B33D9C" modified="2023-09-13" version="1">MELYEWDQGSHRGTPGLRLVLKLSYEHLHLTPGLRMKVSLAAQVMSKTVANALEMTRKHGLQSTIFIRNVNKFFDCPSVGQTTSRAVTAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CPR6</accession>
    <name>A0A9Q1CPR6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Helix-turn-helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_00921</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CPR6"/>
    <dbReference type="OrthoDB" id="9907881at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058912">
        <property type="entry name" value="HTH_animal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21301">
        <property type="entry name" value="REVERSE TRANSCRIPTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21301:SF10">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26215">
        <property type="entry name" value="HTH_animal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helix-turn-helix" evidence="1">
        <location>
            <begin position="92"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF26215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8048560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="154" mass="18062" checksum="C21E356DDDC49241" modified="2023-09-13" version="1">MSKLDTDFFSNYAGLGQPISYIRYIEDIFFIWPHGAEKLDGFVQAFNAFHLSIKFTCEHSNESVSFLDCIVRLDGNSLVTSVYTKPTDKHTYLAYGSFHPKHLKESIIYSQFLRYKRICSTQEAFVENSAQLFHYFQSRGYPFRTLLKYFNRVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CQI7</accession>
    <name>A0A9Q1CQI7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Chromodomain Y-like protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_02309</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CQI7"/>
    <dbReference type="OrthoDB" id="6282662at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00024">
        <property type="entry name" value="CD_CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46585:SF1">
        <property type="entry name" value="CHROMO DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46585">
        <property type="entry name" value="INTEGRASE CORE DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chromo" evidence="1">
        <location>
            <begin position="62"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8049526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="101" mass="12095" checksum="240B6295A075A001" modified="2023-09-13" version="1">MVARPFLKGYLPRWTEELVTVAQRLPRLPVVYRLKYCEGEWLEGTFYEAELQKVTKDGSDVYRVERIVRTRKTKDGKTEYFVKWKGYPSKFNTWVSNLIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CQT2</accession>
    <name>A0A9Q1CQT2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Polycomb group protein Pc</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_01284</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CQT2"/>
    <dbReference type="OrthoDB" id="1918685at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035102">
        <property type="term" value="C:PRC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023779">
        <property type="entry name" value="Chromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052458">
        <property type="entry name" value="PcG_PRC1-like_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46389">
        <property type="entry name" value="POLYCOMB GROUP PROTEIN PC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46389:SF3">
        <property type="entry name" value="POLYCOMB GROUP PROTEIN PC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00598">
        <property type="entry name" value="CHROMO_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chromo" evidence="4">
        <location>
            <begin position="266"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="125"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="197"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="134"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="148"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="214"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8048814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="322" mass="35604" checksum="05E72A2998E9E051" modified="2023-09-13" version="1">MPHHKGKINTNFVQNKKQRLKYKNRRIKTLLSKGMELCSMTNCSVFIYTKDELNHIRVAGSQDMLAAYHGAGIKSTMFSETESTSSEIVQGPTARPSAPTCSSNIMTCDAATACTSSSTASQQDKPAVALVCRRSHESEPHRSHGDNSPMPKTSPSATTTSDSSVCNVPPRPTSSTACVEPTQEEFQLQNASTLLASENNLPHTGPDTGRHSPLNHTYTQPTTQPTEVNSPRPADDSDEEDADEAPLSLVKAVLKLQQDDPLAQVYQAEKITDSRMFLGSKQYFVKWKGWSDKHTTWEPEEHIIDKKLIALFLSQQNKEKEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CR60</accession>
    <name>A0A9Q1CR60_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_02758</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CR60"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8049841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="75" mass="8397" checksum="965A739B3752305F" modified="2023-09-13" version="1">MQGIFVQVCNNSSWIYHNFAVSGVVIGVALVRTQATFGEAQQQKLKAVYKLPGVILEKHENACIIPKFFPLFPNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CRE8</accession>
    <name>A0A9Q1CRE8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">G2/M phase-specific E3 ubiquitin-protein ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_03001</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CRE8"/>
    <dbReference type="OrthoDB" id="2384350at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="HECT" evidence="3">
        <location>
            <begin position="36"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8049998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="213" mass="23547" checksum="C1FC8CBD5F800ED9" modified="2023-09-13" version="1">MAVQRPVQLSDAPSSNNINVLRECVIEGARRAINRRKFDPLKKIRVAFIDADGNEEGSIDNGGPTREFFRLLMQKVMESTCFEGPPDARELALSTKAVDQKEYKNVGAFIALSIIHGGPGPVSMSECLFDELTGTPAIPQLDSISDDYVKNQLSRIKHAGNVDEARSAVVESLDLLSILGTSRYIGSLDERDQLVHDAVRFYRFGRLHSAIDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CRS7</accession>
    <name>A0A9Q1CRS7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Immune-associated nucleotide-binding protein 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_04254</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. AIG1/Toc34/Toc159-like paraseptin GTPase family. IAN subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CRS7"/>
    <dbReference type="OrthoDB" id="8954335at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006703">
        <property type="entry name" value="G_AIG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045058">
        <property type="entry name" value="GIMA/IAN/Toc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903:SF184">
        <property type="entry name" value="GTP-BINDING PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903">
        <property type="entry name" value="GTPASE, IMAP FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04548">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIG1-type G" evidence="4">
        <location>
            <begin position="5"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8050882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="264" mass="30055" checksum="CADF0E2B8008CBE2" modified="2023-09-13" version="1">MDSATVLVLGNTGHGKSLLGNRLLRLNPYDDTKFHFRTSDNELPETMESEHFSAVIDGFELTVIDTPGLFDIEMEVMTRWIGVLKGILLTKKGIDAIIFVANSQRLFTAARCFNRITKCFDGLNMTRVIFVFTYVIDTSARNLEDNLTDFNKELTNGAVGVRHIFSDQCAREDVVERNMSDIISYVKDIKRKSVAPINKAVFVKYTGEYLRHFLQSLPQKNVSLGDLRNSPAIDMLVIEKFIKKEDINKMINERNRSREGCIAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CS51</accession>
    <name>A0A9Q1CS51_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_03093</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CS51"/>
    <dbReference type="OrthoDB" id="5376140at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33480">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33480:SF5">
        <property type="entry name" value="SI:DKEY-51D8.9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8050050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="69" mass="7752" checksum="B405DB3DE105AAF6" modified="2023-09-13" version="1">MATTCQILILKRAELESLAGVMGHNIRVHENFYRLPEGTLQVAKISKIPLAIESGNISKERMENLVVYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1GXH2</accession>
    <name>A0A9Q1GXH2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Kinesin-like protein KIF25</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_32669</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1GXH2"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015630">
        <property type="term" value="C:microtubule cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47972:SF63">
        <property type="entry name" value="KINESIN FAMILY MEMBER 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47972">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KLP-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0505">Motor protein</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="8">
        <location>
            <begin position="173"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="534"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="37"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="125"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="269"/>
            <end position="276"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8027509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="630" mass="70605" checksum="37B87FB522DC71D7" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H0Y2</accession>
    <name>A0A9Q1H0Y2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Carbamoyl-phosphate synthase [ammonia], mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_27586</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8031002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H0Y2"/>
    <dbReference type="OrthoDB" id="434at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004088">
        <property type="term" value="F:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CPAse_ATP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11405:SF53">
        <property type="entry name" value="CARBAMOYL-PHOSPHATE SYNTHASE [AMMONIA], MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11405">
        <property type="entry name" value="CARBAMOYLTRANSFERASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carbamoyl phosphate synthase ATP-binding" evidence="4">
        <location>
            <begin position="12"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS00867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8031002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="77" mass="8712" checksum="36CFD0FD60F045C5" modified="2023-09-13" version="1">MQFLAKKNDVMVIECNLRASRSFPFVSKTIICDMINIATKAMIGEHFDQSLLPLLNNSFTPEDYVGIKAPMFSCPRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H315</accession>
    <name>A0A9Q1H315_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Collagen alpha-1(IV) chain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_27169</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
            <location evidence="1">Basement membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10071882at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005604">
        <property type="term" value="C:basement membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005581">
        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030020">
        <property type="term" value="F:extracellular matrix structural constituent conferring tensile strength"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.240.10:FF:000001">
        <property type="entry name" value="Collagen IV alpha 1 chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.240.10">
        <property type="entry name" value="Collagen IV, non-collagenous"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001442">
        <property type="entry name" value="Collagen_IV_NC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036954">
        <property type="entry name" value="Collagen_IV_NC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050149">
        <property type="entry name" value="Collagen_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24023">
        <property type="entry name" value="COLLAGEN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24023:SF1106">
        <property type="entry name" value="VIKING, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01413">
        <property type="entry name" value="C4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00111">
        <property type="entry name" value="C4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51403">
        <property type="entry name" value="NC1_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0084">Basement membrane</keyword>
    <keyword evidence="6 11" id="KW-0176">Collagen</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040382520" evidence="9">
        <location>
            <begin position="29"/>
            <end position="1743"/>
        </location>
    </feature>
    <feature type="domain" description="Collagen IV NC1" evidence="10">
        <location>
            <begin position="1517"/>
            <end position="1741"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="45"/>
            <end position="1520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="51"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="91"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="199"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="331"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="379"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="413"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="476"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="492"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="537"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="647"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="686"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="718"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="778"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="838"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="948"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="980"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1071"/>
            <end position="1080"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1203"/>
            <end position="1216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1264"/>
            <end position="1273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1335"/>
            <end position="1344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="1346"/>
            <end position="1355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1393"/>
            <end position="1411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1439"/>
            <end position="1449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1478"/>
            <end position="1487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="1503"/>
            <end position="1515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8030690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1743" mass="170246" checksum="4EC1C7AC4CD757E4" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H3R5</accession>
    <name>A0A9Q1H3R5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_24591</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H3R5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8031410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="139" mass="16310" checksum="36242AEE3AF41ACE" modified="2023-09-13" version="1">MNQECIKFICKRVADSSPRVEENLARWVLFPMSLFAIWKDFFLGISIIEKNSEFENQEFIEFICKRAADLSKRGDEKLARWVSSCPSEQVALSSQSRYLKIFRFSLGIFLDIEKNSEFENQEFIEFICERAADLSKRGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H4Q9</accession>
    <name>A0A9Q1H4Q9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Monocarboxylate transporter 9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_22875</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8032818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H4Q9"/>
    <dbReference type="OrthoDB" id="6499973at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008028">
        <property type="term" value="F:monocarboxylic acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF172">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="21"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="58"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="89"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="113"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="148"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="343"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="381"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="409"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="436"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="471"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="502"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="4">
        <location>
            <begin position="22"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="198"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="557"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="225"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="240"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="257"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8032818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="579" mass="64341" checksum="FDBB4FC30205F670" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H5K7</accession>
    <name>A0A9Q1H5K7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_23355</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H5K7"/>
    <dbReference type="OrthoDB" id="6764948at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF7">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8033196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="169" mass="19213" checksum="E1FEB78C938C16FD" modified="2023-09-13" version="1">MESSKAQPAEPVQPHEITTRPWQVLGTDLFTLDGSDHLIVADYYSKYTLIKSIPKGRKTGADSKTNYQEGKQSKTDFHPAMLSLRVTPIDNHLPSPGELLFNRKIRWSLPTKIQNTQPDRDKLSERLVERQSQQRINFDKGTWHLSPMIPGQVVRARPHLTSLETGTSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H668</accession>
    <name>A0A9Q1H668_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Fibrinogen-like protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_24280</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H668"/>
    <dbReference type="OrthoDB" id="6275059at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005577">
        <property type="term" value="C:fibrinogen complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005201">
        <property type="term" value="F:extracellular matrix structural constituent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034116">
        <property type="term" value="P:positive regulation of heterotypic cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037579">
        <property type="entry name" value="FIB_ANG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024731">
        <property type="entry name" value="NELL2-like_EGF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040941">
        <property type="entry name" value="GGGWT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47221">
        <property type="entry name" value="FIBRINOGEN ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47221:SF5">
        <property type="entry name" value="FIBRINOGEN C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12947">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00186">
        <property type="entry name" value="FBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="6">
        <location>
            <begin position="61"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8033900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="233" mass="26702" checksum="E454D508505FE967" modified="2023-09-13" version="1">MFCLKEGVVYIYSDCSRRCSCINGRFSCHHYECSANAACAKRKNVSQCICNAGYTGDGINCTIDTPPSDCQDIYDRVSTESGIYKIKPTTWHGEQFDVYCNMTDGGGWTVFQRRIDGSQDFNLYWSDYKNGFGSIDLESWLGNDKLHSLTAQKGYQLRIDLVDSLGIPYFAKYNSFRINSESDKYRLSVSSYSGNAGLYFLQLFEGLMNKTCLTLQHFWWCRREVNKIGWLIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H6N5</accession>
    <name>A0A9Q1H6N5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SCAN box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_21216</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H6N5"/>
    <dbReference type="OrthoDB" id="8962223at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF13">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCAN box" evidence="2">
        <location>
            <begin position="220"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="78"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8034396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="288" mass="34665" checksum="6739F301D1364BCB" modified="2023-09-13" version="1">MPFNLDKFVASPSVEELDSLKKSEIVKVAKHYGIEFQPLMRKDEIKRYVLEYLVDESILPSTVLETAITVPTDNTFELKRLEIEMNKEIRLKEMEREREREEREREMQMQKEKEEREMQMQMQREKEASDPKIGLDTGGFDVSKHVKFVPKFQEDNVEKFFNHFEKLGEQLKWPRDKWSILIQSNFTGKAQEVYSSLSIEDSMDYDKVKKAILQAYELVPEAYRQKFRKYRKADTQTYVEFAYQKERHFDRWCASKKVSTFDTLRQLMLVEEFKRCVNDDIKTHLEEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H8Q3</accession>
    <name>A0A9Q1H8Q3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_20795</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H8Q3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="100"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="122"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8036735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="261" mass="29235" checksum="495C832F746BE62B" modified="2023-09-13" version="1">MISLDLCEGSTIASPVSHIDRSKRSDEGDVIPILVPPTNVSLYSSASHLKATTLERFQSNIATGTDFTGEIVQTGFDDDQQTPKQLTREERATRSRSRTRSSGSRSSKRTSTSTSNRSRHSMISSGDIFSTPSRRTGGSHRCRTGMCDEGRPTIVRVVRSMRRQLYRLLSRILGEEFTPPESLTEDIILTLLAFLRQHGGEDGQNFLNRVRTTHSAMVTVWEQCCQTYSTGRRKRSTSFKLDQPVDLSELAVTLIVYSIQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H963</accession>
    <name>A0A9Q1H963_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">GTP-binding protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_17883</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H963"/>
    <dbReference type="OrthoDB" id="248233at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04165">
        <property type="entry name" value="GTPBP1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03694">
        <property type="entry name" value="GTPBP_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03708">
        <property type="entry name" value="GTPBP_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000014">
        <property type="entry name" value="Probable GTP-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000091">
        <property type="entry name" value="Probable GTP-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050055">
        <property type="entry name" value="EF-Tu_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035531">
        <property type="entry name" value="GTPBP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43721">
        <property type="entry name" value="ELONGATION FACTOR TU-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43721:SF9">
        <property type="entry name" value="GTP-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="5">
        <location>
            <begin position="140"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="587"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="619"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="653"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="677"/>
            <end position="687"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8037138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="687" mass="74901" checksum="AFD8BBACB39ABA36" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H9P7</accession>
    <name>A0A9Q1H9P7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_11817</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H9P7"/>
    <dbReference type="OrthoDB" id="6283140at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8041097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="72" mass="8887" checksum="55055319421F00B7" modified="2023-09-13" version="1">MPKFLTTNYLINIEEQWASFRDTVYPIALEHLRPAKRKHQDWFDEKNKDIHNLLTEKKRLMRMHQDDPIFSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HA51</accession>
    <name>A0A9Q1HA51_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_16090</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HA51"/>
    <dbReference type="OrthoDB" id="427518at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015631">
        <property type="entry name" value="CD2/SLAM_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12080:SF48">
        <property type="entry name" value="IMMUNOGLOBULIN SUBTYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12080">
        <property type="entry name" value="SIGNALING LYMPHOCYTIC ACTIVATION MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040380622" description="Ig-like domain-containing protein" evidence="5">
        <location>
            <begin position="23"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="354"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8038624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="500" mass="57251" checksum="7949474653F227D6" modified="2023-09-13" version="1">MGVLKQLILLVTVANLADITVSTCLSTQYIEKGVMTSLKCVFPTNFECVVWYKIPGEGPFIKLDRGTKSGPGYESGDYDISSTGRLQINNVSLEDDRFFRVVVLYNKEWEDDVREDIRVHVYVKPLQTFPKIDICGDESYCLTKIDKQIEPACSVIQSRPTVRLQWLNIRGEERELESRTDIQQFDYTASSVAVTTVHIAEGNFLEVLLCRALVTAPVMEQSDAMVLLEGNTKEIPGREIDSQLVEIHDQVLLDIQLDDIYAVVWKKFSYLSFEYETIGHYAHGKSTTLRPDFLIKADGSLQIKNLSLQHEGEYICIYARPLEQDTRKYIVEVFKLRSFFYVYHVFVLSVLPSPPFTTIYGCTERDTCQMDIAETGSLTCTLKGIRPVVHVEWFYPGKGYPPIRFINPKTSIKRKGPLFDIRVTSYFEIVQDRFGGDVNVSCRALGLSEKYHQMSSSITLRLISGMFTHMYNNIQYCNVDRTFLNYAMKERLIVVCTEHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HBD2</accession>
    <name>A0A9Q1HBD2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Torsin-2A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_16678</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HBD2"/>
    <dbReference type="OrthoDB" id="19623at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010448">
        <property type="entry name" value="Torsin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10760:SF2">
        <property type="entry name" value="LD13476P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10760">
        <property type="entry name" value="TORSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8039076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="175" mass="20479" checksum="163494A21DAA9FA8" modified="2023-09-13" version="1">MLQTFTKEKNIICRRMYVFDEVDSLPDNLLTTVASFMVSPFFSKTIFVFLSDVAGQEIYEKIETLEMHNFTKDQIDHNKLVKVVQDAVVNSPPKYDSAMVQHIVPFLPLERDHVAKCIRKEITQRIPFIREKDLIRLTEMVLKELLFHGFQDKYSKKGCKNVVEKVNLVLARDRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HGM2</accession>
    <name>A0A9Q1HGM2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">DNA 5'-3' helicase</fullName>
            <ecNumber evidence="16">5.6.2.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">DNA 5'-3' helicase FANCJ</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">HOLleu_11632</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">Nanhai2018</strain>
            <tissue evidence="21">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DEAD box helicase family. DEAH subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.3" evidence="16"/>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8044233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HGM2"/>
    <dbReference type="OrthoDB" id="19182at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016818">
        <property type="term" value="F:hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990918">
        <property type="term" value="P:double-strand break repair involved in meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18788">
        <property type="entry name" value="SF2_C_XPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000731">
        <property type="entry name" value="Fanconi anemia group J protein homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006555">
        <property type="entry name" value="ATP-dep_Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045028">
        <property type="entry name" value="DinG/Rad3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472">
        <property type="entry name" value="DNA REPAIR DEAD HELICASE RAD3/XP-D SUBFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472:SF47">
        <property type="entry name" value="FANCONI ANEMIA GROUP J PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13307">
        <property type="entry name" value="Helicase_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00491">
        <property type="entry name" value="HELICc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="13" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9" id="KW-0347">Helicase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="14" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-dependent helicase C-terminal" evidence="20">
        <location>
            <begin position="121"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="325"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="397"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="486"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="624"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="704"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="831"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="333"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="638"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="714"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="19">
        <location>
            <begin position="841"/>
            <end position="850"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00044969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00082714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM00491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAJ8044233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1072" mass="118282" checksum="2DC3E5333FD3CA29" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HI08</accession>
    <name>A0A9Q1HI08_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Fimbrin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_08602</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HI08"/>
    <dbReference type="OrthoDB" id="9451669at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="159"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="195"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="75"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="75"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="96"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="125"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8045568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="250" mass="28730" checksum="89183C615FA855B7" modified="2023-09-13" version="1">MATEELFTGDNATVTEEQNGERSEATKEFLHLLKEKEGIKNELFEMEKQFNQAQGRNYELLQKIDELEKELSATKEQLKKEKESKPALPLTPKVQRAARPRTAKHANPRLKQRADPRKNITKSSIDVKKEKEAEKPAKEKDTYKYYQEIAEKYPMLRLSVLLEAEKKFVEGDVNGDGTIDAGELEKILDSGQLMFTKQQVLDIVKEIDTDNSNSLDFVECLKVIDRLQQNKKTNLPTELERNKSTVCSIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HJV8</accession>
    <name>A0A9Q1HJV8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Neuronal acetylcholine receptor subunit alpha-9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_06776</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HJV8"/>
    <dbReference type="OrthoDB" id="5975154at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005230">
        <property type="term" value="F:extracellular ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18989">
        <property type="entry name" value="LGIC_ECD_cation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19051">
        <property type="entry name" value="LGIC_TM_cation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000028">
        <property type="entry name" value="AcetylCholine Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040259164" evidence="6">
        <location>
            <begin position="23"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="256"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="286"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="316"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="498"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="7">
        <location>
            <begin position="35"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="8">
        <location>
            <begin position="263"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8047713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="518" mass="58989" checksum="1EE4E43F97D72A5D" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0Y8P0</accession>
    <name>A0A9Q0Y8P0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Sushi-repeat-containing protein SRPX</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_44664</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010001091">
        <property type="protein sequence ID" value="KAJ8017722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0Y8P0"/>
    <dbReference type="OrthoDB" id="10066368at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003410">
        <property type="entry name" value="HYR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043555">
        <property type="entry name" value="SRPX-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46343">
        <property type="entry name" value="HYR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46343:SF2">
        <property type="entry name" value="SUSHI_VON WILLEBRAND FACTOR TYPE A_EGF_PENTRAXIN DOMAIN-CONTAINING 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02494">
        <property type="entry name" value="HYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50825">
        <property type="entry name" value="HYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="HYR" evidence="3">
        <location>
            <begin position="98"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8017722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="189" mass="20352" checksum="45FF8D976618DFCD" modified="2023-09-13" version="1">MPTVGSEVTCLANDNGSGRWEGTFLTVCDPVKCVPFDPPEHGFIQGCSHEGMGTDTTESQVFPSVCTAACDDGYTPFGSVNRNCLISGIWDGLPLDCLDVTAPSIVCPDPITEFAQPRNNFVVSDFSWEPLQVVDVSPPVDVRLLSINSVGEGENRTTVFTEGLYSFLYLAVDQEGNEDTCTVDLTVRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YCK4</accession>
    <name>A0A9Q0YCK4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_42821</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000141">
        <property type="protein sequence ID" value="KAJ8018915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YCK4"/>
    <dbReference type="OrthoDB" id="10536593at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8018915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="298" mass="33435" checksum="7D319A9BBAC584DA" modified="2023-09-13" version="1">MFRRVETIILLALCTDTTVNVKILEGQIQATWEDGHSGYFAVPKERVEAKLAELQQHKSCHDARLTVPNRPILPEILQPDEHVVAIAYGKLGAKSIEDSLPSNVKAKYETKAATVYFFIPFLTGPNRYAEPKYKLALGSFVSTTKPFIHPYIAIPMEQVDLLEVRSDGLSNVDANEVLNITWSQLCECKEPDEIILHDFEEYIDEFTTSFKINPDFSFCDEPGAGNNIYCKYRNITDCACQFWTHGHLKSCEAKNLSVINVTGKFKEVGLLIDGPQDAVLTEINEGLHGIGVQKHCVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YCY6</accession>
    <name>A0A9Q0YCY6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Echinoidin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_44706</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010001130">
        <property type="protein sequence ID" value="KAJ8017697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YCY6"/>
    <dbReference type="OrthoDB" id="418245at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050111">
        <property type="entry name" value="C-type_lectin/snaclec_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22803">
        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8017697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="145" mass="16899" checksum="36069A13A0E3AB55" modified="2023-09-13" version="1">MFNHMHFVFHSHLKVVRITTFESELCRYCRATVVTAWSKSKMHFCKDFIVILVICMGCVECHQCNTEEKNHDAACPTYWASRGNFCYRFYSTEMTWVDAEAFCNTIGAPNGQGIAHLLSVHSADEQKWLYDYWKSHRNETPTNVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YDG8</accession>
    <name>A0A9Q0YDG8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">MPN domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_41348</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase M67 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8019674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YDG8"/>
    <dbReference type="OrthoDB" id="167806at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08067">
        <property type="entry name" value="MPN_2A_DUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000053">
        <property type="entry name" value="MPN domain-containing protein CG4751"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000555">
        <property type="entry name" value="JAMM/MPN+_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050242">
        <property type="entry name" value="JAMM_MPN+_peptidase_M67A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040843">
        <property type="entry name" value="RAMA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10410">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01398">
        <property type="entry name" value="JAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18755">
        <property type="entry name" value="RAMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102712">
        <property type="entry name" value="JAB1/MPN domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MPN" evidence="8">
        <location>
            <begin position="251"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="139"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="168"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00061577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8019674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="479" mass="54349" checksum="2FE6E9440F9B6415" modified="2023-09-13" version="1">MDRVEAVMKEDETNSDAESVESSSSSPRRKRSQFLTGRGVTLRMLMEDGIVEAGEGVLSIDYLGQKFTADLLDDGRIRWTEENMIFNSPSSWAIHIKKLVNPHKRSGCGWSSVKYKGQKLDLVKSRWFCKLNLNKMGLRNGNGDGESGSDYGSDEEESEKPNPPMSDFVKECIKNLQDGKEKQENDDEKENKENEGGLCNSHSGNVISSLYPIKKVEQYSDLGKRSQDIPTSTLVECKSFKSLGKMQPFTISVTSNSLLLIDFHCHLTTSEVVGYLAGKWDPTSQHMSVLQAYPCKCRLGDREGSRKVEGEIQQSIAMRGLQLVGWYHSHPCTAAHPTIQDIDSQMDFQVRMKGEGNTYQPCLAMICNPYNLQRAVKESTLRSFWVMPSPEVSDPEKHYGMPVNMSYSCQQDMFLTQDVLVELKSLVEFYRNTPDMVYFGETWHQDDTFLSKLKDSLAKKFPKDQTDDRLFDYVNELLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YFB1</accession>
    <name>A0A9Q0YFB1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RNA methyltransferase</fullName>
            <ecNumber evidence="6">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_38581</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the methyltransferase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="6"/>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YFB1"/>
    <dbReference type="OrthoDB" id="10017101at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017069">
        <property type="term" value="F:snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040031">
        <property type="term" value="P:snRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039772">
        <property type="entry name" value="Bin3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010675">
        <property type="entry name" value="Bin3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024160">
        <property type="entry name" value="BIN3_SAM-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12315:SF0">
        <property type="entry name" value="7SK SNRNA METHYLPHOSPHATE CAPPING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12315">
        <property type="entry name" value="BICOID-INTERACTING PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06859">
        <property type="entry name" value="Bin3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51515">
        <property type="entry name" value="BIN3_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Bin3-type SAM" evidence="8">
        <location>
            <begin position="348"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="51"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="265"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="610"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="655"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="67"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="78"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="619"/>
            <end position="634"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8021400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="679" mass="76496" checksum="E5158557DBE67220" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YFB8</accession>
    <name>A0A9Q0YFB8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Nucleolar complex protein 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_38994</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NOC2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YFB8"/>
    <dbReference type="OrthoDB" id="10266662at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030690">
        <property type="term" value="C:Noc1p-Noc2p complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030691">
        <property type="term" value="C:Noc2p-Noc3p complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005343">
        <property type="entry name" value="Noc2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12687">
        <property type="entry name" value="NUCLEOLAR COMPLEX 2 AND RAD4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12687:SF4">
        <property type="entry name" value="NUCLEOLAR COMPLEX PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03715">
        <property type="entry name" value="Noc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="680"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="23"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="35"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="92"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="121"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="137"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="162"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="680"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="703"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="740"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="763"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="807"/>
            <end position="818"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8021713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="840" mass="95923" checksum="5F1CC6C00851C683" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YFL4</accession>
    <name>A0A9Q0YFL4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="23">Hyalin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">HOLleu_40778</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="23">Nanhai2018</strain>
            <tissue evidence="23">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8021024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10051896at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000003">
        <property type="entry name" value="Transmembrane serine protease 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.960">
        <property type="entry name" value="SEA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003410">
        <property type="entry name" value="HYR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000082">
        <property type="entry name" value="SEA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036364">
        <property type="entry name" value="SEA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24273">
        <property type="entry name" value="FI04643P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24273:SF32">
        <property type="entry name" value="HYALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02494">
        <property type="entry name" value="HYR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01390">
        <property type="entry name" value="SEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00261">
        <property type="entry name" value="LDLRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00200">
        <property type="entry name" value="SEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82671">
        <property type="entry name" value="SEA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50825">
        <property type="entry name" value="HYR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50024">
        <property type="entry name" value="SEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
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</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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</entry>
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</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YI41</accession>
    <name>A0A9Q0YI41_HOLLE</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_42524</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000083">
        <property type="protein sequence ID" value="KAJ8019111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YI41"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8019111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="159" mass="17386" checksum="3168E745FD029869" modified="2023-09-13" version="1">MPHIEKKNPIVFGGGQRSPGVTGGQTLKTLLTQYLEMRYGMRKNPIIFGGGERPSGVTGGQTLKTSLTRYLKMPHTKRKNPIVFGGDRRSPGVTGGQTLKTLLTQYLEMRYGMRKNPIIFGGGERSSGVTGGQTLKTSLTRYLKMSHIKRKNSIVFGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YI49</accession>
    <name>A0A9Q0YI49_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Reverse transcriptase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_40766</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8021014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09274">
        <property type="entry name" value="RNase_HI_RT_Ty3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000115">
        <property type="entry name" value="Polyprotein of retroviral origin, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000032">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 297-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon gypsy-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054465">
        <property type="entry name" value="Integrase_p58-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041373">
        <property type="entry name" value="RT_RNaseH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22938">
        <property type="entry name" value="Integrase_p58_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17917">
        <property type="entry name" value="RT_RNaseH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="10">
        <location>
            <begin position="864"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase" evidence="9">
        <location>
            <begin position="1278"/>
            <end position="1455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="333"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="597"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="697"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="78"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="333"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="348"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="597"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="641"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="666"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="713"/>
            <end position="737"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8021014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1670" mass="190807" checksum="58B2440398827620" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YIM6</accession>
    <name>A0A9Q0YIM6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_37863</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YIM6"/>
    <dbReference type="OrthoDB" id="6781885at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33776">
        <property type="entry name" value="ENDO/EXONUCLEASE/PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33776:SF4">
        <property type="entry name" value="ENDONUCLEASE_EXONUCLEASE_PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="1">
        <location>
            <begin position="15"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8022854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="446" mass="50887" checksum="823D72753A39BB93" modified="2023-09-13" version="1">MCNFLTGINYSFSTIAVSETWVSDNEIKSLYNIPGYNFVCKGRDSRRGGGVLKDGMNYSVRNDFENTQHCESLFIEVKHAYEKITIVGVIYRPPDGNVGDFNDELQVILDRISREVKWGFLAGDFNIDLLSHVSSSFKDNFENLLSSHCFYPTITYPTRVTDSTATLIDNVFTNHHNLKLKSSIFETDISDHYPICVYCGETSVAVQTGADYDIFKNDTKTPNLLKFKNRIASYDWAEVYSERDPNSAFTTFLSCLLESFNRNCPVRKVTKTKYLRKSPWITNGISKSISTKSKLFYKYRSNPTKENSTAYRNFRNTLTKIIRYAKSMYYVDYFDKNLRNSRRIWSGIRQITKGVGNPTQSYFSIDGGVTSNASDIAEHLISYFSTIATDLASKIKPTNKPYSAYLTCHIQNTFAFNPVTHEEVLLAICKLLSHKSPGYDNISSEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YIW3</accession>
    <name>A0A9Q0YIW3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_41225</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8019576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YIW3"/>
    <dbReference type="OrthoDB" id="2221171at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="entry name" value="Intergrase catalytic core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8019576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="145" mass="16158" checksum="4716C58604A317CF" modified="2023-09-13" version="1">MKQTPWLFSTDNITFLPSGDAGIVFHGRKNDASRTGFHVTLRPTRETKIDPVKALRDYIARTSHQRSEDKCVFLSLNPPFCPVTAKTVANILNEAINIVGLSGRGFSAKSFRPTGATLAVESVFYEHYVHAKPPETFSEKILLHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YLC7</accession>
    <name>A0A9Q0YLC7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_35875</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YLC7"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040138002" evidence="1">
        <location>
            <begin position="23"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8023430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="309" mass="34328" checksum="364A2C070D772103" modified="2023-09-13" version="1">MHRSLLQIFLVGQGLLYFGASAIVVENISSSAWDGVGIYISVKRETIQKKLDLFNSPVSSKCGDGPKLFLPDGAVFPDLHNGQHPVYLDVGFLTSHDFDQYLANNRDPCIKVSGLEVSVTIPFLAGNSGGPPIYALAQAKFYEKSRGDFPKTNFLYPYIPVDEIKMDDFVAVVKNGSDEMRFSFNRLNLSCKPPVSIVTKEFDGFVLNDLQFGLSEPEFSFCDRHSFVNDYCHNAARVRCDVHRNAPNVCLIWRSGNEKPCITVTTVEIQNITSGLREIIPVGDDPINVFASESFSGDYTVGLKYPCQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YN94</accession>
    <name>A0A9Q0YN94_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">MHD2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_33216</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10071880at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099503">
        <property type="term" value="C:secretory vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014772">
        <property type="entry name" value="Munc13_dom-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052095">
        <property type="entry name" value="UNC-13_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45999:SF6">
        <property type="entry name" value="MHD2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45999">
        <property type="entry name" value="UNC-13-4A, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51259">
        <property type="entry name" value="MHD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MHD2" evidence="3">
        <location>
            <begin position="1123"/>
            <end position="1234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1318"/>
            <end position="1340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1359"/>
            <end position="1380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1392"/>
            <end position="1534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1618"/>
            <end position="1640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1322"/>
            <end position="1336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1400"/>
            <end position="1410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1411"/>
            <end position="1426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1436"/>
            <end position="1479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1519"/>
            <end position="1530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1621"/>
            <end position="1630"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8025614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1722" mass="193998" checksum="917EB05D38C8FF97" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YQG5</accession>
    <name>A0A9Q0YQG5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Heparan sulfate 2-O-sulfotransferase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_31601</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sulfotransferase 3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YQG5"/>
    <dbReference type="OrthoDB" id="10019582at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007734">
        <property type="entry name" value="Heparan_SO4_2-O-STrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005331">
        <property type="entry name" value="Sulfotransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12129">
        <property type="entry name" value="HEPARAN SULFATE 2-O-SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12129:SF15">
        <property type="entry name" value="URONYL 2-SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03567">
        <property type="entry name" value="Sulfotransfer_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8026692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="394" mass="45700" checksum="DF68F08A121CDAA5" modified="2023-09-13" version="1">MENMFGNFGNIERNSQNTVRTGTGILNLKAILTTNRSKSLDNLTDSKALGSFEETQLATERYEHSRDGELLGIVNMTANTTVREEVVIQGKSSDIVFTKETDYKQCNSSLDPSCMFGKCITGTNHILFYNRVPKCGSRTFLFLAKVLASKNSFHKPREATNSVKKKRKDSNPVDGAALKTTLRRRLMNMPLGSFLHGHIKYIDFKNDTRLPEKKQPIYLNIIRDPIDRLVSQYYFVRYGDRLFTNKRRLRKLQKNIFLNETLNDCVERRRPECCGSNAQVQYFCGYHPDCQRREEAFQRAVRNLDYYLVVGITEEYEDFLKVLEKLLPDYFKGVVDEYNTPAETNRKTQSSARKKQPLTEAVRDKLRSLLDMEYKFYYHVRSKFQGLKQELGIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YRY3</accession>
    <name>A0A9Q0YRY3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">TWiK family of potassium channels protein 9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_34176</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the two pore domain potassium channel (TC 1.A.1.8) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8026355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YRY3"/>
    <dbReference type="OrthoDB" id="297496at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015271">
        <property type="term" value="F:outward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022841">
        <property type="term" value="F:potassium ion leak channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030322">
        <property type="term" value="P:stabilization of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003280">
        <property type="entry name" value="2pore_dom_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11003:SF330">
        <property type="entry name" value="POTASSIUM CHANNEL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11003">
        <property type="entry name" value="POTASSIUM CHANNEL, SUBFAMILY K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01333">
        <property type="entry name" value="2POREKCHANEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5 8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="34"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="202"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="227"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="254"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="11">
        <location>
            <begin position="19"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="11">
        <location>
            <begin position="207"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="154"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF07885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8026355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="309" mass="33707" checksum="F85E34BC8472ACE2" modified="2023-09-13" version="1">MDVLRIFILGGHCDYHDSYGHIAPVTTAGRIFCMAYAIFGIALLLLVLASIGSLLARGATLTYRMLHGKIGAFTIGKRKDNTADDPYLRHNGAPSRQDEHREKVIDDIWGTIKFDKDGKMIMPEDTNARQASASATPDEEKGANASHYKVTFEDGGKANSEAGKGHKTTPSPGPSGEIEIERSIGIGAIDDPDDEVNVPLHVVLVLACIYIVILAGTLRLWESQWDFFDALYFSFVTLTTIGFGDLVPQHQKNLLACTFLIILGMAIISMCIALAQEFIMKKIAWLSQKVGVALLRSSKGDDNKSLASH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YS40</accession>
    <name>A0A9Q0YS40_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">RWD domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_31321</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YS40"/>
    <dbReference type="OrthoDB" id="277175at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.400.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040213">
        <property type="entry name" value="GIR2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032378">
        <property type="entry name" value="ZC3H15/TMA46_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12292">
        <property type="entry name" value="RWD DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16543">
        <property type="entry name" value="DFRP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ZC3H15/TMA46 family C-terminal" evidence="3">
        <location>
            <begin position="45"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="74"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="16"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="127"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8026490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="143" mass="16898" checksum="C8C626BE942B6180" modified="2023-09-13" version="1">MRCMRVRFLELISASQEFLNERVDDIKKEKEKQLEKEEEEKRKAEEERAQLLKGTPVTIETFLTWKEKFDKEMAEMTKKNHQQEGPTKRLTGKELFMSDNALDTSDMDLLDESDKVQVDESLFQDMGDLDLEEELDLEDDDES</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YSV0</accession>
    <name>A0A9Q0YSV0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">BMP and activin membrane-bound inhibitor-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_35188</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8025081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YSV0"/>
    <dbReference type="OrthoDB" id="5914644at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="CDD" id="cd23576">
        <property type="entry name" value="TFP_LU_ECD_BAMBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045806">
        <property type="entry name" value="BAMBI_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045807">
        <property type="entry name" value="BAMBI_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06211">
        <property type="entry name" value="BAMBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19337">
        <property type="entry name" value="BAMBI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040313495" evidence="2">
        <location>
            <begin position="39"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="158"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="BMP and activin membrane-bound inhibitor N-terminal" evidence="3">
        <location>
            <begin position="36"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="BMP and activin membrane-bound inhibitor C-terminal" evidence="4">
        <location>
            <begin position="142"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF06211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF19337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8025081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="316" mass="35602" checksum="9185051474D1870F" modified="2023-09-13" version="1">MPVCTMNLQRLCTGCMNLQAVLICSLLLLLYSVSDCRGDKVHCYCNSPRCVGYSYMCVSEYGCFAQLATDSSYLRGCVEDLMMIERCLQAEENTFKRQNKSLIRAEENIDLGFPLLSCCYDDMCNYANYDQQDQRSSYNSDHEQRKNEEMTSLNSKAVWFRAAVIAVPIAGVCILILLAMLASRLLSNDANKETFSPLNNQRLPYPQKRTHAGPCHGQPYMQQKRSLYFNSRVPISDNYSQRGGGVRSSLPADVEQEQLRFARTFAESDGLLTGSLGAAEGQFGLPKCTNNSQFLSHQHIKATQFYPSRTDKLQIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YSZ3</accession>
    <name>A0A9Q0YSZ3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Acyl-CoA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_35278</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ACBP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8025156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YSZ3"/>
    <dbReference type="OrthoDB" id="346910at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000062">
        <property type="term" value="F:fatty-acyl-CoA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000582">
        <property type="entry name" value="Acyl-CoA-binding_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035984">
        <property type="entry name" value="Acyl-CoA-binding_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23310:SF62">
        <property type="entry name" value="ACYL-COA BINDING PROTEIN 1, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23310">
        <property type="entry name" value="ACYL-COA-BINDING PROTEIN, ACBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00887">
        <property type="entry name" value="ACBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00689">
        <property type="entry name" value="ACOABINDINGP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47027">
        <property type="entry name" value="Acyl-CoA binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51228">
        <property type="entry name" value="ACB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ACB" evidence="3">
        <location>
            <begin position="2"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8025156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="84" mass="9366" checksum="D09D2E44CB9E8EBB" modified="2023-09-13" version="1">MPSDEFNKAAEDAKKLTVKPSDSDLLELYGLFKQVTVGDVNTERPGMFDMKGKAKWDAWNSRKGVSASEAEQLYIAKVKQLQGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YTT1</accession>
    <name>A0A9Q0YTT1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uronyl 2-sulfotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_32306</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sulfotransferase 3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YTT1"/>
    <dbReference type="OrthoDB" id="10019582at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007734">
        <property type="entry name" value="Heparan_SO4_2-O-STrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005331">
        <property type="entry name" value="Sulfotransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12129">
        <property type="entry name" value="HEPARAN SULFATE 2-O-SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12129:SF15">
        <property type="entry name" value="URONYL 2-SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03567">
        <property type="entry name" value="Sulfotransfer_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="15"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8027224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="464" mass="53657" checksum="93CDECA93CE20826" modified="2023-09-13" version="1">MGDQTFRKMVWKRKFLYLVCVGSVVACMIFINTNKDEPVSFSHSRLQASEYVIASTEGIESPSVQQSVSGGQNIKGRFHHSLLPTVRLSRTNLPTFNQSFSTKTETLMRHFSNSSSKASDMNRNFFINSSIINLEAGNQGKLSSSEIFYKAPNRTLLSLILGDKNDQARSINNHYNRVFQWDDRDPEGWRGLPFSKGEVAPKTDRIIFNRVGKCGSRSMIAMLRDLAQQNGFNLISSVVYNQTHVPKLREKHIVSILDQVQPPYLFQRHIHYVNFKRHMAQEVVYINIIREPIARMVSQYYFARYGDGILGKARAKWNRPLEEFNETIDECVKHEHRDCSGHRTFYIIPFFCGNSKRCVTPSKWALQRAMYNVQNNFLVVGILEEFDLTLRILETVLPTFFAGAMELYQTSANVAENITKTTTSFKIPPSKETIEILKERLKYEYKFYNFVKQRLYEQKARLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1B950</accession>
    <name>A0A9Q1B950_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">RNA 3'-terminal phosphate cyclase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_45108</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010001862">
        <property type="protein sequence ID" value="KAJ8017465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1B950"/>
    <dbReference type="OrthoDB" id="1911237at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000479">
        <property type="term" value="P:endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.20">
        <property type="entry name" value="RNA 3'-terminal phosphate cyclase, insert domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013791">
        <property type="entry name" value="RNA3'-term_phos_cycl_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000228">
        <property type="entry name" value="RNA3'_term_phos_cyc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020719">
        <property type="entry name" value="RNA3'_term_phos_cycl-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013792">
        <property type="entry name" value="RNA3'P_cycl/enolpyr_Trfase_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036553">
        <property type="entry name" value="RPTC_insert"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11096">
        <property type="entry name" value="RNA 3' TERMINAL PHOSPHATE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11096:SF1">
        <property type="entry name" value="RNA 3'-TERMINAL PHOSPHATE CYCLASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05189">
        <property type="entry name" value="RTC_insert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55205">
        <property type="entry name" value="EPT/RTPC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01287">
        <property type="entry name" value="RTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="domain" description="RNA 3'-terminal phosphate cyclase insert" evidence="2">
        <location>
            <begin position="80"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8017465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="133" mass="15164" checksum="871ADD7321A6DF45" modified="2023-09-13" version="1">MQHFYCSEHLFRIHQLVSLIFFIFKVDIIRAVSLQVMKKFGIDEGLELTVSKRGSPPHGGGEVIFKCPVCRNLRPLQFVKAGKVKRIRGVVFSTRVSPAFVNRLVEGARSILNKFLTDIYIYTDHMKGPRSGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BA65</accession>
    <name>A0A9Q1BA65_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">THAP-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_44464</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000909">
        <property type="protein sequence ID" value="KAJ8017868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BA65"/>
    <dbReference type="OrthoDB" id="6616463at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006612">
        <property type="entry name" value="THAP_Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31751:SF42">
        <property type="entry name" value="PROTEIN CBG10204"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31751">
        <property type="entry name" value="SI:CH211-108C17.2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50950">
        <property type="entry name" value="ZF_THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="THAP-type" evidence="7">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="188"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="256"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="188"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="280"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="302"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="323"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8017868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="831" mass="94251" checksum="9E444983E9E51699" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BB00</accession>
    <name>A0A9Q1BB00_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_38408</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BB00"/>
    <dbReference type="OrthoDB" id="10445382at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8021261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="252" mass="29083" checksum="912E74966E6F8D0D" modified="2023-09-13" version="1">MRNITPILDTGEVAVYFQQPAEYKQYFVCPLCPTTENLYKCVKDKRTILLGDSHVRQIPNINTSEFSWHFLQHGPGIYRNLATKTKDNYEKYKNGSIQASFSPGAMLTSLRKGSYKDLQEQYHFDTWVISSGHWDLRDDTVESYITHLEELLKEWTQFQSQYFVQVIWNGVPSFSYKAKSLGGLERRTNVKIAVADAKTKLLTSKYNITYVPFFDLSYPFYSYSCDTHHYLCPSQSSPIGSVQLNTLLNAIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BCN9</accession>
    <name>A0A9Q1BCN9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">BRCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_41495</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8019772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BCN9"/>
    <dbReference type="OrthoDB" id="5997366at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="CDD" id="cd23767">
        <property type="entry name" value="IQCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRCT" evidence="1">
        <location>
            <begin position="929"/>
            <end position="1035"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8019772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1059" mass="120812" checksum="4DDBF8CA18934B72" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BCV1</accession>
    <name>A0A9Q1BCV1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">LINE-1 retrotransposable element ORF2 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_39279</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BCV1"/>
    <dbReference type="OrthoDB" id="7762009at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31635">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31635:SF196">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8021935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="253" mass="29860" checksum="DCA50B6FBFBE2A68" modified="2023-09-13" version="1">MRNILNEFQYASGLKINLGKSHLFPLGPLILNQPEYLCDFGITPSFGPIKFLGISFNHNGDKLFRLNYLQKLSRLKSILHVWSMRDLTPIGKNIIVKSFALSQFVFLFQVLPDPPDYFIKDVQGCIFDFIWSCNPDKVRRNTMYNSIENGGLKITHVRSFISALKIIWVKRYRDEDDRIWKLFFDYYLRRYRGQFLFDCNFSSQDVIGIKNVFIRNVCCAWGTMNFHHPHDNFDSQILWNNSYININKTIVFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BD10</accession>
    <name>A0A9Q1BD10_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Cytochrome b-c1 complex subunit 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_39716</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII), a multisubunit enzyme composed of 11 subunits. The complex is composed of 3 respiratory subunits cytochrome b, cytochrome c1 and Rieske protein UQCRFS1, 2 core protein subunits UQCRC1/QCR1 and UQCRC2/QCR2, and 6 low-molecular weight protein subunits UQCRH/QCR6, UQCRB/QCR7, UQCRQ/QCR8, UQCR10/QCR9, UQCR11/QCR10 and subunit 9, the cleavage product of Rieske protein UQCRFS1. The complex exists as an obligatory dimer and forms supercomplexes (SCs) in the inner mitochondrial membrane with NADH-ubiquinone oxidoreductase (complex I, CI) and cytochrome c oxidase (complex IV, CIV), resulting in different assemblies (supercomplex SCI(1)III(2)IV(1) and megacomplex MCI(2)III(2)IV(2)).</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII), a multisubunit enzyme composed of 3 respiratory subunits cytochrome b, cytochrome c1 and Rieske protein, 2 core protein subunits, and additional low-molecular weight protein subunits. The complex exists as an obligatory dimer and forms supercomplexes (SCs) in the inner mitochondrial membrane with cytochrome c oxidase (complex IV, CIV).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the UQCRB/QCR7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BD10"/>
    <dbReference type="OrthoDB" id="425749at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045275">
        <property type="term" value="C:respiratory chain complex III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006122">
        <property type="term" value="P:mitochondrial electron transport, ubiquinol to cytochrome c"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1090.10:FF:000001">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1090.10">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003197">
        <property type="entry name" value="QCR7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036544">
        <property type="entry name" value="QCR7_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12022:SF0">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12022">
        <property type="entry name" value="UBIQUINOL-CYTOCHROME C REDUCTASE COMPLEX 14 KD PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02271">
        <property type="entry name" value="UCR_14kD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000022">
        <property type="entry name" value="Bc1_14K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81524">
        <property type="entry name" value="14 kDa protein of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0249">Electron transport</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 12" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="4 12" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00046393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR000022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8020192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="110" mass="13133" checksum="B311DE093FD87816" modified="2023-09-13" version="1">MASNVAASSGRLLRWMQKTYYNLAGFNQMGLMTDDTLRLTPDVVEAVRRLPPDVYNQRAFRIKRALDLSLKHRILPQEEWTKFEEDVRYLKPYLEEVQREMKEKAEWNAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BEL9</accession>
    <name>A0A9Q1BEL9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Arp2/3 complex 34 kDa subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_34998</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Functions as actin-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the Arp2/3 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cytoplasm</location>
            <location evidence="1 6">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the ARPC2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BEL9"/>
    <dbReference type="OrthoDB" id="148331at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005885">
        <property type="term" value="C:Arp2/3 protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030041">
        <property type="term" value="P:actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034314">
        <property type="term" value="P:Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1460.20:FF:000002">
        <property type="entry name" value="Arp2/3 complex 34 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1460.20:FF:000004">
        <property type="entry name" value="Arp2/3 complex 34 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1460.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007188">
        <property type="entry name" value="ARPC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034666">
        <property type="entry name" value="ARPC2/4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12058:SF0">
        <property type="entry name" value="ACTIN-RELATED PROTEIN 2_3 COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12058">
        <property type="entry name" value="ARP2/3 COMPLEX 34 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04045">
        <property type="entry name" value="P34-Arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69645">
        <property type="entry name" value="Arp2/3 complex subunits"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="280"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="281"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU364015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8024936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="301" mass="34754" checksum="FBC2464CCA6F5E9E" modified="2023-09-13" version="1">MILLEIKNKIVEETLLQRFESAANGGKADAIDIKVGDFDGVLYHISNPNGDRTKITVSASLKFFPELQKYGADELLKREYGDLIVETEQEYDVTLQFDCQNLPANKEELAAKAALLKRNCFASVFERYFQYLENEDTSGRERAVIHYREDETMYVHAKKDRVTVVFSTRFKDDDDIIIGKVFMQEFKEGRRASRNAPQVLFSHRDPPLELEGTDASTGDNIGYITFVLEPRHTSESVRDNTINLIHTFRDYLHYHIKCSKAHIHSRMRARTTDFLKILNRARPEDQSKEKKTMSGKTFARS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BFC3</accession>
    <name>A0A9Q1BFC3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_34444</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BFC3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8024515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="81" mass="9179" checksum="B683C470516DC87F" modified="2023-09-13" version="1">MVKAQNKFDLTTNLRNADKSLVGTVITAIIHLSTAVGLSQRFATNREIERQLLGTSKSDLDSTVWIPDWLEEASIFRIYLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BFR2</accession>
    <name>A0A9Q1BFR2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_33812</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8026076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BFR2"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="6">
        <location>
            <begin position="3"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="68"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8026076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="90" mass="9922" checksum="21BC13B8DA1749FD" modified="2023-09-13" version="1">MATEYCIHCSGTVQEEEKAIQCDGCSRWHHLGCNTGVTEEEYAGAVEEEGVIEWACVWCSSKAVEEGQPVAESTKVDAEPEEYDIPQPIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BG80</accession>
    <name>A0A9Q1BG80_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Fatty acyl-CoA reductase</fullName>
            <ecNumber evidence="1">1.2.1.84</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_36707</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reduction of fatty acyl-CoA to fatty alcohols.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a long-chain fatty acyl-CoA + 2 NADPH + 2 H(+) = a long-chain primary fatty alcohol + 2 NADP(+) + CoA</text>
            <dbReference type="Rhea" id="RHEA:52716"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:77396"/>
            <dbReference type="ChEBI" id="CHEBI:83139"/>
            <dbReference type="EC" id="1.2.1.84"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the fatty acyl-CoA reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.84" evidence="1"/>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8024079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BG80"/>
    <dbReference type="OrthoDB" id="429813at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102965">
        <property type="term" value="F:alcohol-forming long-chain fatty acyl-CoA reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080019">
        <property type="term" value="F:alcohol-forming very long-chain fatty acyl-CoA reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035336">
        <property type="term" value="P:long-chain fatty-acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026055">
        <property type="entry name" value="FAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013120">
        <property type="entry name" value="FAR_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11011:SF45">
        <property type="entry name" value="FATTY ACYL-COA REDUCTASE CG8306-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11011">
        <property type="entry name" value="MALE STERILITY PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07993">
        <property type="entry name" value="NAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="1" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioester reductase (TE)" evidence="2">
        <location>
            <begin position="16"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8024079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="111" mass="12575" checksum="0B9A6D309E4B24DD" modified="2023-09-13" version="1">MNSPVADYFAGKTIMLTGGTGFIGKVLVEKILRCCPDVDKFYLLIRPKAGQDPQQRIKKEFVECKRIKGSSTVVVAERDIERYPNTSSRDTIESRRYSAVRLLTESYEASH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BGV8</accession>
    <name>A0A9Q1BGV8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Charged multivesicular body protein 1b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_32314</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SNF7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BGV8"/>
    <dbReference type="OrthoDB" id="10266568at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005024">
        <property type="entry name" value="Snf7_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10476">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03357">
        <property type="entry name" value="Snf7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="164"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="164"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8027231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="191" mass="21341" checksum="2EDDFB03E7D44398" modified="2023-09-13" version="1">MFNLKFAAKNLERQSKKCEKEQKAERLKLKKAIEKGNLEGAKIHAENSIRQKNQALNFLRMSARVDAVATRVQTAVTMKNVTQSMAGVVKSIDSAMRSMNLEKVSALMDKFEKQFEDLDVQSSYMESAMSNTTTMTTPQSDVDNLMQEVADEAGLELNMELPQGQTSAIGQASASQEQDELSQRLAELRKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BHV1</accession>
    <name>A0A9Q1BHV1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_31578</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BHV1"/>
    <dbReference type="OrthoDB" id="10051619at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="137"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="281"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="448"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="352"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="496"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="18"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="163"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="287"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="454"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8026673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="852" mass="97547" checksum="0C5E75582CE825E5" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BHY8</accession>
    <name>A0A9Q1BHY8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_33793</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8026059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BHY8"/>
    <dbReference type="OrthoDB" id="10680965at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003903">
        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50330">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="101"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="55"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="8"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="123"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="173"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="201"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="216"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="237"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="294"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8026059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="327" mass="37076" checksum="6DE97DE18C68B475" modified="2023-09-13" version="1">MGRKKTSKYNSLNFTSSKPDKNQPTISQSFNKAKEFNDKMDDSDEDVEIITSERSARVERRHKAMALENEEQENEAALLQQEEADLQRAIELSRLEACASGSQEWFTNDVVPDQSRETQINLEEPDEDALLGQEDDSSNEDKKSVKSEILLDSDNEEELPKERESEAEISYNSSRVSDGSSASATFPANHLQRKPPRKEEETLEVLEDVGEDFEEDLPKSGVDEAGSGREGRPSDEFNLSQDFLENQKTSRLSLRKRKRETDEASAPSKTLKVSTDLCEEDYEGETQPMLSNSQKEEDAKQEIENLVNGPFSLVQSEQLEERLPDNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BKC8</accession>
    <name>A0A9Q1BKC8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SCAN box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_30219</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BKC8"/>
    <dbReference type="OrthoDB" id="8963689at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF13">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="78"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8028084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="364" mass="43440" checksum="1026B3E57B2D1387" modified="2023-09-13" version="1">MPFNLDKFVASPSVEELDSLKKCEIVKVAKHYGIEFQPLMRKDEIKRYVLEYLVDESILPSTVLETAITVPTDNTFELKRLEMEMNKEIRLKEMEREREREEKAREHEFRLKQLELGVIKGSDPKTGLDTGVFDVSKHVKFVPKFQEDNVEKFFNHFEKLGEQLKWPRDKWSILIQSNFTGKAQEVYSALSIEDSMDYDKVKRAILQAYELVPEAYRQKFRKYRKADTQTYVEFAYQKERHFDRWCASKKVSTFDTLRQLMLVEEFKRRPKLFQGGECYKTTRQEHRIESIDERMESSREFNFNAAFLELSRSENCLRYVPIIAATWQHFEEAAIIVIQLLCVLEVSIKERSRNYWTVYTRSFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BKU4</accession>
    <name>A0A9Q1BKU4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Dolichyl-phosphate beta-glucosyltransferase</fullName>
            <ecNumber evidence="4">2.4.1.117</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HOLleu_30543</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Nanhai2018</strain>
            <tissue evidence="16">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a di-trans,poly-cis-dolichyl phosphate + UDP-alpha-D-glucose = a di-trans,poly-cis-dolichyl beta-D-glucosyl phosphate + UDP</text>
            <dbReference type="Rhea" id="RHEA:15401"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19502"/>
            <dbReference type="ChEBI" id="CHEBI:57525"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.4.1.117"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:15402"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.117" evidence="4"/>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BKU4"/>
    <dbReference type="OrthoDB" id="3784at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004581">
        <property type="term" value="F:dolichyl-phosphate beta-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04188">
        <property type="entry name" value="DPG_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000068">
        <property type="entry name" value="ALG5, dolichyl-phosphate beta-glucosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035518">
        <property type="entry name" value="DPG_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10859:SF91">
        <property type="entry name" value="DOLICHYL-PHOSPHATE BETA-GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10859">
        <property type="entry name" value="GLYCOSYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="15">
        <location>
            <begin position="69"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00070518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ8028339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="324" mass="37150" checksum="32F80A9BC5204021" modified="2023-09-13" version="1">MVPDLISLLYLGCVIAFVALLVGVAILYMASPPPDLYRYLNEKSYQDPNDDNNFFDFPSLYSPATIDLSVIVPAYNEEERLPKMLDECLDFLEERRKTTKGFSYEVIVVDDGSKDKTTQVALKYSQKYTTEHVRVLKLVKNRGKGGAIRLGMFSARGRALLFADADGATEFKDLTKLEECLKKININEDSRALVCGSRAHLQDESVAQRSFFRTILMYGFHFFVWFLCVKSIKDTQCGFKLLTRNAAAVLFTNMHVNRWAFDVELLYIAQYLNIPMGEVAVNWQEIDGSKIIPVFSWAQMGKDILFIRLRYTLGFWKVEDKKNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BLX6</accession>
    <name>A0A9Q1BLX6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Alpha-N-acetylgalactosamine-specific lectin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_28297</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BLX6"/>
    <dbReference type="OrthoDB" id="418245at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050111">
        <property type="entry name" value="C-type_lectin/snaclec_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22803">
        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040205880" evidence="1">
        <location>
            <begin position="21"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="2">
        <location>
            <begin position="32"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8029018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="178" mass="20921" checksum="7481F82FE324E849" modified="2023-09-13" version="1">MKTKFPVLLVALLSNHFTNGLEVVCPSEWTQWKGGCYRFFNQPRCSWKDAEEFCNKHQLSCDICPGETAHLVTIVSKEENDFLFNWWKSLRDPLPTDTRQTFWIDGNDEQSEGKFVLINGKPLEYSNWIRQQPNNWRNNQDCIAMTKSPIHDSDIGKWNDDKCSLESAFMCEFRCFQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BM87</accession>
    <name>A0A9Q1BM87_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_28467</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BM87"/>
    <dbReference type="OrthoDB" id="6116397at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="134"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="222"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="256"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="312"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="343"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8029139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="376" mass="43412" checksum="1CEEF3E38DFB08AF" modified="2023-09-13" version="1">MVRTRRTTSSVPFSVLSQTCNDENLPPRQHNDEEKASSRGLYIKRNWASSPGVYPHQISRETGREASHVKETPVKSISPGLQKLIQSPGRTYQTQQLLMQKHHDAKSKAEERFVGGGISIWPEMNNMRLTFSDLMTLVANSIILMVLTYGLFTYLHGTTLYHLNYFIKHLKGFPSRHPLETSDQMIFVESVLRWHKDYSKLLKNIDSYLGVSWETSPSTYPLAIFLYILGIAILLFYLYDSVFSKSRLTPQRIKQWVCLLVILMTWTILMLYLLLRAQKLENTVKMNVHFLNGYMEELVLTELDLSKYESVLHFWTTMCLLPSSEGLLTVLGIITVQNLTYYLIYYSVPIVTALFTPILQLIISLYEIYKVKPHPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BMR5</accession>
    <name>A0A9Q1BMR5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_28680</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BMR5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="174"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="187"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="198"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8029315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="249" mass="30134" checksum="3321FCDD19DFC9CA" modified="2023-09-13" version="1">MKVAILHYEASDTGIRKQKRLDLSQEERKKERKKERKKERKKERKKERKKERKKERKKERKKERKKERKKEKKEGREGGREGGREGGREGGREGGRKEGRKEGRKEGRKEGRKEGRKEGRKEGRKEGRKEGRKEGRKEGRKEGRKDGRKEGRKEGRKEGRKEGRKEGRKEGRKEGRKGKERKGKERKGKEGRKEERKKERKKERKKERKKERKKERKKERKKERKKDWIPAYLHPPSLFPRPGYTGLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BNZ7</accession>
    <name>A0A9Q1BNZ7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Perilipin-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_29570</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lipid droplet</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the perilipin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8030006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BNZ7"/>
    <dbReference type="OrthoDB" id="376826at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019915">
        <property type="term" value="P:lipid storage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010890">
        <property type="term" value="P:positive regulation of triglyceride storage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.340">
        <property type="entry name" value="Flagellar protein FliS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004279">
        <property type="entry name" value="Perilipin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14024:SF49">
        <property type="entry name" value="LIPID STORAGE DROPLETS SURFACE-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14024">
        <property type="entry name" value="PERILIPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03036">
        <property type="entry name" value="Perilipin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036881">
        <property type="entry name" value="PAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109775">
        <property type="entry name" value="Mannose-6-phosphate receptor binding protein 1 (Tip47), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="172"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="286"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="179"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR036881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8030006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="426" mass="47520" checksum="9A85E800A7BF21D7" modified="2023-09-13" version="1">MADESRDNNSAPSEGTEHNSEESAITRITNLPIVTDALTSVSNAYQWTKDSNALIGYSLDVAEKSVAMAAYTAQPVVNCLERPISAVNSMANQSLDKLEEKVPLITEPSDQVVKSLKESAWMAVEQSKATGTQKMNDLMNTRVGQVVTSTVDAALSLSERAVDYYLPEEHVEEAKERDEGVEDDISDEESNAISEEGAGTRSKEPFSNSVNKASKVSNKVRRRLYYKALVNIQYAQKRSQETLQKLHFTVDLIQYAKENIDSANSGIRSKVDSTQQTLWNTWNDWTVDPKEEGQSNNEDEDVEAKTGEQKTLAIARNLTRRLRVLTNNLSTSIRVLPENLRKSVEETRGTVEMLFNTFEEAQYFKDLPSSILNTAKERLVQLSEGSVALADYLVNSPAMQWLVPSSVRQLVTGIHTRAISEKEKML</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BPT9</accession>
    <name>A0A9Q1BPT9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Fatty acid synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_26835</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BPT9"/>
    <dbReference type="OrthoDB" id="3509362at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004312">
        <property type="term" value="F:fatty acid synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05195">
        <property type="entry name" value="enoyl_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050091">
        <property type="entry name" value="PKS_NRPS_Biosynth_Enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43775">
        <property type="entry name" value="FATTY ACID SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43775:SF7">
        <property type="entry name" value="FATTY ACID SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="3" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="1" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="9">
        <location>
            <begin position="48"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8030411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="213" mass="23411" checksum="22EFF0D278DB1BCC" modified="2023-09-13" version="1">MNVFKNGCWGSYRHQILKEVEHIRTHSTFPEEFSAAPQNAYVDVLTPGDFSSLRWIHSPVTPTSRERCTVHYSSLNFRDVMLASGKLPAEALRGKVIYFVCKDLAHKECLIGIEFAGRNSEGKRVMGVVENQGLASEVFAEPYFLFDVPESWSLEDAATVPAVYCTVYYALVVRGHIKRGDSILIHSGSGGVGQAAIAVALSYGCKIYTTVGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BPU1</accession>
    <name>A0A9Q1BPU1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sulfotransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_27117</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BPU1"/>
    <dbReference type="OrthoDB" id="10010208at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053259">
        <property type="entry name" value="Golvesin-related_Golgi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32301">
        <property type="entry name" value="COUNTIN RECEPTOR CNR3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32301:SF6">
        <property type="entry name" value="GOLVESIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8030647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="298" mass="34512" checksum="8980C824C09E7D26" modified="2023-09-13" version="1">MNFPIVHLRNYLPNAYLYPQLAKPYNTSVLTFLHNPKSGGTSVRDCMLGLYRAMEKDRPVVVATNTVSGEKEKLLNHVTQTNDYYMGDAALGICDTFEDRPCSYFTVVREPYERMVSHYYFCKSGGPGWPPCDNTLEEFTMSLCSLFFRQLTVQVFCKEDNQNPSSPWKCDRMPVSIDHLPEDQQQRAAILDYFLANVDKMFAVIGLTEEFETSLKLFENTFGEPFYGLCNEKHSNAGSYKNVTDTDDTKADRERITAEAKERLMNNEQIRQCLHEDVQLYNKMYEIFQKQKQYSNIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BPX7</accession>
    <name>A0A9Q1BPX7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_27090</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BPX7"/>
    <dbReference type="OrthoDB" id="10656692at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040347521" description="Ig-like domain-containing protein" evidence="2">
        <location>
            <begin position="37"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="485"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="361"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8030625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="575" mass="64769" checksum="7F2B6393D3893C72" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BQK4</accession>
    <name>A0A9Q1BQK4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">15-oxoprostaglandin 13-reductase</fullName>
            <ecNumber evidence="2">1.3.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_27435</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.48" evidence="2"/>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BQK4"/>
    <dbReference type="OrthoDB" id="9992527at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047522">
        <property type="term" value="F:15-oxoprostaglandin 13-reductase [NAD(P)+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000121">
        <property type="entry name" value="Prostaglandin reductase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002364">
        <property type="entry name" value="Quin_OxRdtase/zeta-crystal_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051397">
        <property type="entry name" value="Zn-ADH-like_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43677:SF3">
        <property type="entry name" value="PROSTAGLANDIN REDUCTASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43677">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE/REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01162">
        <property type="entry name" value="QOR_ZETA_CRYSTAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="4">
        <location>
            <begin position="7"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8030893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="250" mass="26888" checksum="75306A6237DE0437" modified="2023-09-13" version="1">MDGTRTGTMSSIRKMATLPSSFRKLVVTNFSNNFRQATEIQTSLGEVVAVGSNVKHLSVGQPVACMNMGSYSEYQVIPSKSAIPLPDLKPEYIALMASGITADLAFTECGPLKEGQKVLVTAAAGGTGQFAVQLAKLAGCHTIGTCSTDQKAAFLKNIGCDRPINYNKEDLKTVLRTEYKDGVDVIYESVGGDVFDICFNALARHGRLLVIGAISSYNFDAKPHSSSVLPRLPLSYMYSGKNEGKIYVQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BT60</accession>
    <name>A0A9Q1BT60_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Leucine-rich repeat-containing G-protein coupled receptor 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_25680</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BT60"/>
    <dbReference type="OrthoDB" id="676979at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366">
        <property type="entry name" value="IG(IMMUNOGLOBULIN) AND LRR(LEUCINE RICH REPEAT) DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366:SF96">
        <property type="entry name" value="LEUCINE RICH REPEAT CONTAINING 53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="405"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8032213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="433" mass="49543" checksum="6E43CCD44EC0B740" modified="2023-09-13" version="1">MVQNLYVVHTNLTDLQDGNVTLPYFPNLTTVRLYSNGIRVVNQGLITENPSIQSLFLHTNRLTDVPINALELLTNLRYLDLSGNKITIIKAFTFRWNTFLEKLYLQNNDIQKIEQHALSGLLNLNILGLRNNSMNAFPFDEVRYLPKLNNIYFSRNNVTVLTGKKEVSSTPVQLFDLNQNYLRSFDDYILRIFPSIQVLFVNYNQISHIGPLVIGYKSYNLTVLQISHNTLNEIPVSLLQRLPNLTLLSLKSNRIKIIPQAAFKQNHRLRRIGLSHNQIHFTHEASLKGLHSLEVLNLVYNDLRRLPSGLFDYVDHDFAFFIDGNNFTCDCDTHFLKRWFKKTRFFFGDVNCLIPGSGNYTSLISFEFDMICLEATSIGIFTTDVTMTTTVSLRKNNLHFSLKTAAVSVMIAIALFVIIVIKCVLNRKSTPGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BXW6</accession>
    <name>A0A9Q1BXW6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_21506</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BXW6"/>
    <dbReference type="OrthoDB" id="10649564at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8034599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="389" mass="43295" checksum="DD6ED88616C7AFCE" modified="2023-09-13" version="1">MLRTCGGDVLGRINGNLEEVTVIEVDRFNRSVVIERDDGETLPLPSCLLMELEDDVDKDLFECSMEENSEPHGVQDIWEEGGSYTCAGFVVDGEGAMTDFHISGPTKKVKQLKRSMSRSVKQTLKKASKDAFETSLERSKNNFTYRKAVRAGPLRQLVIGEVEKALNSKARFVIKEFPPQGGEIATSDFHMIELSMVDAKNVHNPPVRIDESIDVFGETYLLVGAAVMQPNHFATVVKVDGNNVLFDGVLRDALVYPTFAAALEGSNSDQPFFFTSPTSKYGVHVLLYRKLVADILSMANIKQAALKDGASYREERIANLENKISKRGVRQGSFCFHFDCNGKLQPEEKKPVLKSGMMEGSLDPGDYIWGYTTEGEKLFKDHAERFSVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BZJ1</accession>
    <name>A0A9Q1BZJ1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NACHT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_22643</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BZJ1"/>
    <dbReference type="OrthoDB" id="8680608at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46844">
        <property type="entry name" value="SLR5058 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46844:SF1">
        <property type="entry name" value="SLR5058 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NACHT" evidence="2">
        <location>
            <begin position="72"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="162"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8035419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="663" mass="76922" checksum="8D8E822813FFA902" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C142</accession>
    <name>A0A9Q1C142_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_20487</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C142"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8036490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="220" mass="23444" checksum="B4A0CFB4ACDCCFE1" modified="2023-09-13" version="1">MDGNLPGFRNDACRISSACSGGLPSGLRGPFMQVPTDSDRLIQGNGDNGTLLHKQSPTGHFSRPDCRFIQPTGPATSPAGNEATPASGFCVDQTKSMRPNQTGSIHCPSVELTANPYQDAAHRMGLTTTGHQMRYPGSPAQMYNAAAAAGQTASQYAPHLSYINYPSYANPAAGYPFGMAIPTLLDGCLHWQRFPDGQSVGHIPNFKFSNLKKNSKLNST</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C3P7</accession>
    <name>A0A9Q1C3P7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_19011</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8038042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C3P7"/>
    <dbReference type="OrthoDB" id="10614283at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8038042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="187" mass="21208" checksum="C320C4F83894B846" modified="2023-09-13" version="1">MQQLKSELLDLDVFCKGSFRRDIRLLRETASKLDSARAHVSDNLGLYSSRAGLVGGTMMLAGLIPNPFSPVLFYYGSYLSMSSAIGHVSNVVLVEESYDLVEVAQVIQNLNKFYEVMLRVEDAYDALLKLQPEAILRGKNGRTLSKEKQQKILLEIEKLWDKKVNLEEKRKITLAETIDYTNSLIDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C3Q8</accession>
    <name>A0A9Q1C3Q8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Doublesex- and mab-3-related transcription factor A2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_18713</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C3Q8"/>
    <dbReference type="OrthoDB" id="6162476at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007548">
        <property type="term" value="P:sex differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1040.10">
        <property type="entry name" value="DM DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001275">
        <property type="entry name" value="DM_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036407">
        <property type="entry name" value="DM_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026607">
        <property type="entry name" value="DMRT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12322">
        <property type="entry name" value="DOUBLESEX AND MAB-3 RELATED TRANSCRIPTION FACTOR DMRT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12322:SF116">
        <property type="entry name" value="DOUBLESEX-MAB RELATED 99B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00751">
        <property type="entry name" value="DM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00301">
        <property type="entry name" value="DM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82927">
        <property type="entry name" value="Cysteine-rich DNA binding domain, (DM domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS40000">
        <property type="entry name" value="DM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50809">
        <property type="entry name" value="DM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="DM" evidence="7">
        <location>
            <begin position="18"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="DM" evidence="5">
        <location>
            <begin position="18"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="90"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="250"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="90"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8037807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="270" mass="31056" checksum="C6F66C8299EB18FC" modified="2023-09-13" version="1">MEAIMEPSALRHKTVPKCAQCRNHNQIVPRKGHKNRCPWSKCTCRKCTLTFEKRQLSATRQRMRREPLEDDETEQQDEVQLLLNLKSTIEKKEKKKESHDTASEGKQKYRYSGNIQNNMYFPANPQPQETVKPKDSEGVSGSPPVDLVTPSGYNNDLSWPPPCPFCPCNHWEMVLPPTQQMPTFNPEGCQYMPHQLPNAWASPYNHYIEHSFHNPESLVTRFPASSVSQPPESNSMFQYQPFTTAQYSFSNGPLNLQSNNPRFASRDQTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C4T6</accession>
    <name>A0A9Q1C4T6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_16914</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C4T6"/>
    <dbReference type="OrthoDB" id="6150661at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8039258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="232" mass="27378" checksum="87A64270BE92A34A" modified="2023-09-13" version="1">MALGELGRHKIAKTIEMRMISFWLRIINGSERKLSFTIYKLLRALHDKGIYSSPWILKIKNILDSSGMSYIWNDPYDINIPWVKKTLNLRLSDMYEQNWLSEVYENSQCKNYRIFKSRLVLEKYLLSLSNRDRLSLCSFRCGNTKIPAIAGRFLNIDLNDRLCELCTSGSIGDEFHYLFQCTAFKSDREHFLKRYYRVGPNTLKMAQLLTTRNKRVQRNLATFCSIISNRFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C6V8</accession>
    <name>A0A9Q1C6V8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Anion transporter 4, chloroplastic</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_14713</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C6V8"/>
    <dbReference type="OrthoDB" id="2250022at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015291">
        <property type="term" value="F:secondary active transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006820">
        <property type="term" value="P:monoatomic anion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17380">
        <property type="entry name" value="MFS_SLC17A9_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000452">
        <property type="entry name" value="sialin-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050382">
        <property type="entry name" value="MFS_Na/Anion_cotransporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044777">
        <property type="entry name" value="SLC17A9-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11662:SF40">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11662">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="45"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="99"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="134"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="163"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="216"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="253"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="294"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="9"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8040428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="405" mass="43932" checksum="1C2D8603DADA9D5A" modified="2023-09-13" version="1">MKMSKTQYLIFLCGMANFINAADRVIMPIAIVPMSDLYHWDLQLQGWILSAFAYGYISSQVLGGKAAQRFGGRAVLGIAVACWSFATFITPMVASQVHIVIFLRILLGFAEGFCLPTIFHVFSCSILLEERSKAFGYLVAGGSVGQCLASVICPYMSWPDMFYTFGAIGFVWVLLWFMLYTKEMDGVNSDPQKLMEQKVDTTSQVNWRSYISHRPLWAIYFAHFCMNTSNYIISTWLPTYLQKTLGANPHDISFTALPFIANSIVGVVAGHRADYLIAKGSWTVLSVRRLMTGIGLLGPAVFVLLFGSVSSVAVAISYISISLGLCACNSAGHLSNHGDVAPHHSGITFAVSNTLATLPGLTAGPVTAAAVDYFGTWTPVFLAVSLVNVAGAVVYITESSASQVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C6Z2</accession>
    <name>A0A9Q1C6Z2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Neural proliferation differentiation and control protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_16604</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C6Z2"/>
    <dbReference type="OrthoDB" id="6270617at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009635">
        <property type="entry name" value="NPDC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23352:SF2">
        <property type="entry name" value="NEURAL PROLIFERATION DIFFERENTIATION AND CONTROL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23352">
        <property type="entry name" value="NEURAL PROLIFERATION DIFFERENTIATION AND CONTROL PROTEIN-1 NPDC-1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06809">
        <property type="entry name" value="NPDC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8039021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="155" mass="17420" checksum="FDB6D3063DF932DF" modified="2023-09-13" version="1">MDDIGDMFEKAKDYVSNARRTAKDAIFNAKEYFYANYQTDTIIQTASKDAEAEYPSYGVTGPGAAMSGDRKLAQSAQMYHYQHQKQQMIAMQKREKGESAEDASDYDSEEENEEGDLTVYECPGLAPTGEMQVKNPLFSEEQYKEATPPPAEEKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C910</accession>
    <name>A0A9Q1C910_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Histamine N-methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_14468</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C910"/>
    <dbReference type="OrthoDB" id="5984880at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8040236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="292" mass="33203" checksum="4A0CB5A20B089BFC" modified="2023-09-13" version="1">MSLRCLQNFPSHYWKSYRSMRDKFYKCPLEYDIGEEAFQKMVVDVLPKQESLKTLGIGSGQGELDCINMDRMLTRVSHIDNTVVEPSLEAMDVYKSLILGDLSDRVTTSWYQETFQQYQKRRGSSGSTKKFHFISAVHSLYYTGKDGSIMGYLIDLLEKDGVLFIAIQNDESGFTKFFGRVAESYGPLFNNDTQGYTTSQVAKLLDADGRVTYNVLKNRHNIDITACFEADSSTGSLLLDFLAHIVDFTSTISKNVLESLLDYLKTPDCSEILSDGRIIFHEDWDVIIITKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CA36</accession>
    <name>A0A9Q1CA36_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Lipopolysaccharide-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_12418</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CA36"/>
    <dbReference type="OrthoDB" id="10035901at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd22343">
        <property type="entry name" value="PDDEXK_lambda_exonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.15.10.10">
        <property type="entry name" value="Bactericidal permeability-increasing protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.15.20.10">
        <property type="entry name" value="Bactericidal permeability-increasing protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017943">
        <property type="entry name" value="Bactericidal_perm-incr_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001124">
        <property type="entry name" value="Lipid-bd_serum_glycop_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017942">
        <property type="entry name" value="Lipid-bd_serum_glycop_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011604">
        <property type="entry name" value="PDDEXK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019080">
        <property type="entry name" value="YqaJ_viral_recombinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47526">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47526:SF3">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01273">
        <property type="entry name" value="LBP_BPI_CETP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02886">
        <property type="entry name" value="LBP_BPI_CETP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09588">
        <property type="entry name" value="YqaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00328">
        <property type="entry name" value="BPI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55394">
        <property type="entry name" value="Bactericidal permeability-increasing protein, BPI"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040185470" evidence="5">
        <location>
            <begin position="20"/>
            <end position="1006"/>
        </location>
    </feature>
    <feature type="domain" description="SWIM-type" evidence="7">
        <location>
            <begin position="488"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="6">
        <location>
            <begin position="940"/>
            <end position="1000"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8041561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1006" mass="113188" checksum="16BFBD5975D9CEED" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CAA5</accession>
    <name>A0A9Q1CAA5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Methylthioribose kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_15495</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methylthioribose kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8041015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CAA5"/>
    <dbReference type="OrthoDB" id="2461at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002575">
        <property type="entry name" value="Aminoglycoside_PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34273">
        <property type="entry name" value="METHYLTHIORIBOSE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34273:SF2">
        <property type="entry name" value="METHYLTHIORIBOSE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01636">
        <property type="entry name" value="APH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminoglycoside phosphotransferase" evidence="6">
        <location>
            <begin position="199"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8041015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="385" mass="44232" checksum="0DAF300878DDB882" modified="2023-09-13" version="1">MDHEKFRHLIRNSSDLPESLAFLKDDELSFEEVGDGNLNDVIRVARKRNPSKSVILKYAPPHIKILGPKYPLGTGRNYLEYRALSAFHNFCPGSVPRPFYCNKTENYAIIEDLKGYRDLRKELIDGVFDVQAALRIADYTLKLHKHSHKCHLPSEEFSKMIEDFRNTEMVDITDQFIFKRPWLQDDPTNKYSLDVQEHLYLVYGNEELLQNVKELQEKFLYQTECLLHGDLHTGSIMTSGGQAKIIDPEFAYVGPAAFDIGMLLANYVFSFYGHCYHPKEGALDFHKTVKTATLNTIKHYFDGMSSWMKEDQFTALVKETTGFAGCELLRRIIGTTRVPELEGRPRAEVTCMRLGLDLIIKHKQVKTVSAMVSSLEMDENKKSSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CAK4</accession>
    <name>A0A9Q1CAK4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Integrase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_12215</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CAK4"/>
    <dbReference type="OrthoDB" id="10061219at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040676">
        <property type="entry name" value="DUF5641"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331:SF5">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18701">
        <property type="entry name" value="DUF5641"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase zinc-binding" evidence="2">
        <location>
            <begin position="629"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="domain" description="DUF5641" evidence="3">
        <location>
            <begin position="850"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF18701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8041407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="906" mass="103406" checksum="10AD47FFB4491C69" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CAT3</accession>
    <name>A0A9Q1CAT3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SCAN box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_12370</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CAT3"/>
    <dbReference type="OrthoDB" id="6077919at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF13">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCAN box" evidence="2">
        <location>
            <begin position="235"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="329"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="329"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="344"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="368"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8041527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="411" mass="48126" checksum="7DD7DD96A7FF1792" modified="2023-09-13" version="1">MPFNLDKFVASPSVEELDSLKKSEIVKVAKHYGIEFQPLMRKDEIKRYVLEYLVDESILPSTVLETAITVPTDNTFELKRLEMEMNKEIRLKEMEREREKEKEEREMQMQKEKEEREMQMQREEKAREHEFRMKQLELGVIRGSDPKVGLDTGVFDISKHVKFVPKFQEDNVEKFFNHFEKLGEQLKWPRDKWSILIQSNFTGKAQEVYSALSIEDSMDYDKVKKAILQAYELVPEAYRQKFRKYRKADTQTYVEFAYQKERHFDRWCASKKVSTFDALRQLMLVEEFKRCVNDDIKTHLEENKADNLKEVANLADQYALTHKFSKVGQTQNKGQFSRPNKKYGNSSGTTGNSGSQSGSISPTNSGKKQPDSSRQKNDQVSSNKAAFKDIIICGFCHKRATQRQIASNWQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CC09</accession>
    <name>A0A9Q1CC09_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_13153</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8042160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CC09"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C-type lectin" evidence="1">
        <location>
            <begin position="10"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8042160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="117" mass="12640" checksum="F2023638CBFDE92D" modified="2023-09-13" version="1">MCYKRLEDPLAFDDARAACQADGADLASASTPAKNTIVSGLASGQRCWVGAIVEAGLGGCPMDPHQGFVLSLPYQVIVNWKQIGHRQQKDECAHQNRVHPMPFPPTPIPTSLLNTPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CCX2</accession>
    <name>A0A9Q1CCX2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">SNF-related serine/threonine-protein kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_13397</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8042365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CCX2"/>
    <dbReference type="OrthoDB" id="942095at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14339">
        <property type="entry name" value="UBA_SNRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057380">
        <property type="entry name" value="UBA_SIK1/2/3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23312">
        <property type="entry name" value="UBA_SIK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="133"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="258"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="133"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="152"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="262"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="278"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="295"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="314"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="325"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="358"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="382"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="431"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="446"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="523"/>
            <end position="538"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8042365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="581" mass="64569" checksum="5086813A3621E7B2" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CD11</accession>
    <name>A0A9Q1CD11_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">E3 ubiquitin-protein ligase MARCHF6</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Membrane-associated RING finger protein 6</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Membrane-associated RING-CH protein VI</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">HOLleu_13463</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="23">Nanhai2018</strain>
            <tissue evidence="23">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Interacts with DIO2. Interacts with SQLE.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8042414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CD11"/>
    <dbReference type="OrthoDB" id="1108038at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16702">
        <property type="entry name" value="RING_CH-C4HC3_MARCH6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000096">
        <property type="entry name" value="E3 ubiquitin-protein ligase MARCH6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056521">
        <property type="entry name" value="MARCHF6-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13145:SF0">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE MARCHF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13145">
        <property type="entry name" value="SSM4 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23113">
        <property type="entry name" value="MARCHF6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12906">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51292">
        <property type="entry name" value="ZF_RING_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="14" id="KW-0007">Acetylation</keyword>
    <keyword evidence="10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="15 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="12" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="95"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="145"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="282"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="323"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="367"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="414"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="472"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="756"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="844"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="887"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="929"/>
            <end position="952"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="972"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="domain" description="RING-CH-type" evidence="22">
        <location>
            <begin position="3"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="176"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="653"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="179"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="20">
        <location>
            <begin position="673"/>
            <end position="684"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00064724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00069012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00082010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00083917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KAJ8042414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1030" mass="115633" checksum="C0F9F465613C8469" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CE43</accession>
    <name>A0A9Q1CE43_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Zonadhesin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_09729</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8042859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="5945029at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19941">
        <property type="entry name" value="TIL"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000055">
        <property type="entry name" value="alpha-tectorin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003410">
        <property type="entry name" value="HYR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022041">
        <property type="entry name" value="Methyltransf_FA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050780">
        <property type="entry name" value="Mucin_vWF_Thrombospondin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036084">
        <property type="entry name" value="Ser_inhib-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002919">
        <property type="entry name" value="TIL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025615">
        <property type="entry name" value="TILa_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014853">
        <property type="entry name" value="VWF/SSPO/ZAN-like_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001007">
        <property type="entry name" value="VWF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001846">
        <property type="entry name" value="VWF_type-D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11339">
        <property type="entry name" value="EXTRACELLULAR MATRIX GLYCOPROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11339:SF373">
        <property type="entry name" value="VWFD DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08742">
        <property type="entry name" value="C8"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02494">
        <property type="entry name" value="HYR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12248">
        <property type="entry name" value="Methyltransf_FA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01826">
        <property type="entry name" value="TIL"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12714">
        <property type="entry name" value="TILa"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00094">
        <property type="entry name" value="VWD"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00832">
        <property type="entry name" value="C8"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00214">
        <property type="entry name" value="VWC"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00215">
        <property type="entry name" value="VWC_out"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00216">
        <property type="entry name" value="VWD"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57567">
        <property type="entry name" value="Serine protease inhibitors"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50825">
        <property type="entry name" value="HYR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51233">
        <property type="entry name" value="VWFD"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040391191" evidence="4">
        <location>
            <begin position="24"/>
            <end position="2108"/>
        </location>
    </feature>
    <feature type="domain" description="HYR" evidence="5">
        <location>
            <begin position="34"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="VWFD" evidence="6">
        <location>
            <begin position="123"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="VWFD" evidence="6">
        <location>
            <begin position="519"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="domain" description="VWFD" evidence="6">
        <location>
            <begin position="927"/>
            <end position="1102"/>
        </location>
    </feature>
    <feature type="domain" description="VWFD" evidence="6">
        <location>
            <begin position="1318"/>
            <end position="1494"/>
        </location>
    </feature>
    <feature type="domain" description="HYR" evidence="5">
        <location>
            <begin position="1720"/>
            <end position="1805"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8042859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="2108" mass="234240" checksum="29AD3F1F8F7E12AF" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CFF2</accession>
    <name>A0A9Q1CFF2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_10833</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CFF2"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8043644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="240" mass="26887" checksum="1EA2E155B0B8F9E5" modified="2023-09-13" version="1">MIRLNRDKLAACWNGPFRVSRVMNNGTTYQLQDLSNLNKPVKILHYNRLKPFCSPYNLGEGGDGNVNIPVQVQVPRLDLQMEIPEDQSEPQAPSTNVSKDNKVTRENPIEVAEATEPSEQPPASPRPPAPPTTVTCKKEVVHRTLILEDMISVFKDSKIMESEIKLIVVGEMGQRRLTCYRQNSCERLQGPQGVPVRFSEAFLVAVLLGEEELTDEIMLTSLNEYVTDAVKELIKRGGTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CG61</accession>
    <name>A0A9Q1CG61_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_07562</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CG61"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8044732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="383" mass="43092" checksum="5E832770D4DC95BD" modified="2023-09-13" version="1">MLIPDSTRIWAGIINFDKIEPSTIPKRSYLSSWGDSRPFRRGPGHPLHKENLCHLRGSSEPSQGPTPVPARLLASFSWGLAVLVKSFRYAGYNMRRNPIVPPSDDFKPSLRPVLYDPSSLQIHEAVPAMVVPATRILSKEDTFLRQQTLLPHCRRTASNTLLGSLPGGTRSTQRAVWSTPRKTSHQHPLELLCVRRAVKTDLHTRYRSPPAPEPLLPGKEPVSESFSPDHIFLPRLCQRLLITVREGIASYAQSGRLSGTLRRLSLLCVRRRSVPDPSDLLTLRHPKIPYPDGLLKSLHPSLLRMTLLKPMMSELMPHLLRGSEEFRLQDIMRAASWEDSIHLCSCHTLPTSSLPRLCLPVPSLEVQLLLQQLLQDLPPSSRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CGE0</accession>
    <name>A0A9Q1CGE0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">AH receptor-interacting protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_11646</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8044243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CGE0"/>
    <dbReference type="OrthoDB" id="5829758at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000052">
        <property type="entry name" value="Aryl-hydrocarbon-interacting protein-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039663">
        <property type="entry name" value="AIP/AIPL1/TTC9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056277">
        <property type="entry name" value="PPIase_AIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013105">
        <property type="entry name" value="TPR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11242">
        <property type="entry name" value="ARYL HYDROCARBON RECEPTOR INTERACTING PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11242:SF0">
        <property type="entry name" value="TPR_REGION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23322">
        <property type="entry name" value="PPIase_AIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07719">
        <property type="entry name" value="TPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 5" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="AIP/AIPL N-terminal FKBP-type PPIase" evidence="7">
        <location>
            <begin position="27"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="5">
        <location>
            <begin position="266"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="314"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8044243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="335" mass="38560" checksum="FBE7AD79CA495C4E" modified="2023-09-13" version="1">MEVGMGMNLRNGITKKVIYPGKGDLPEYHSETKITFHYRCAKLNEGNTVLDDSRVGTGQPMELVTGKQFKLDVWEECLKTMRVQEVADFFVDRSHLTVFPAVMKTLRDVRKGGQAHNHKDSHCCGLAKMNKEGLGYDDLDALVKSAEHLVFRFEVLKVENPGSYKQETWAMSDEERKKILPTLKEEGNNYYKNKDYQKAADKYAEALGCLENLLLHEKPNSEDWLILDSVRIPFLLNFAQCKYQLGEYYQAIEHATSVLDKEEDNVKALFRRGQAHRHCCNYQEARNDLLKAAKLDPKLGAVVRKELQAIEEMEKERDAEEKEKLAGLFDKIREH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CKW5</accession>
    <name>A0A9Q1CKW5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_06427</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CKW5"/>
    <dbReference type="OrthoDB" id="6259957at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21301:SF11">
        <property type="entry name" value="GIY-YIG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21301">
        <property type="entry name" value="REVERSE TRANSCRIPTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8047432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="110" mass="12867" checksum="7C60D505D54B0803" modified="2023-09-13" version="1">MFEMYIFCLQWRVYLQIQGAGMGSPVSPTICTLYIEHLEFEVIRTAPHQPVWWHRYVDVTRAKLKKKNTHNTFTDHLNTLDLDIKFTTEGEEEKALAFLDTLTVMEDDGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CM16</accession>
    <name>A0A9Q1CM16_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_00265</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CM16"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8048092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="209" mass="24398" checksum="8713F88557805306" modified="2023-09-13" version="1">MIETIFDEQDLAGLRSFDSQIQELYPPLEVPKIEFTVPNLVDNYIELSDTYAADFKKSSKLAQEKLQIFETKVDEGISRTRNFSDQIVDRSGDWKKAFQDLIGGIFGKDVWIVLTFICSPAGLTIVAFVLNATIFIPEFTVFFIEYREHRKHRNYEEETLKLLKDSKQNNPSNSNNYSLLFDLKSRIYTRENEKAGWYLKQKTNENKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CMN1</accession>
    <name>A0A9Q1CMN1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_06490</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CMN1"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.30">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="150"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="161"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8047480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="474" mass="53170" checksum="F497454846E7E71E" modified="2023-09-13" version="1">MFAKFIQTFQAVILLAIICKIDARYRSYPRYRPPPVMTMTNYEIRFFPRQSYCLNLLKVIEDTGGYNGAHVYCQRGLCRYYEGTEKYCATNGKFSKTFRNRCLLCAKGCEELNRGRRMYRSEYTGECRERDPGIATTDASSTLETSIKVTSAPKGPFTKPSTSQSSTHHTTSLKESTYFGTTSILEDVGDSSFTIVVHGSTVKVSTTQPFTSPFTSATTSDSPYLSTEEYTTLESFSITSSTQFTSPYVVPSTSLSVTNTSLSTPSSTLTTDIKTTGIESSFQPHETTIDIEEGKTRDLKTDGMITSQSEVPALSEVPTEDQTTTLTPIFFTGKTDVIGTTLSTGLLHHETTMSSQEGKSIQLETDAFITLETDVPAVSELLTVDLKTKLSSHSFTPVFRTVGGLEVMEPQELQTRCKKERHKLNHFKQRYQINPYLEEHLCVKNYEHFLINCKTIKTRYERLSTSIPDLPDIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CPS5</accession>
    <name>A0A9Q1CPS5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_00940</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CPS5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040216214" description="Ig-like domain-containing protein" evidence="2">
        <location>
            <begin position="29"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="300"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="116"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8048575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="393" mass="45282" checksum="A1E138B18060D126" modified="2023-09-13" version="1">MSEHPLTSNAHLLVELFLVFTFIQYTVCRQNWTVSIYDSVNLTCKSDSRGVRNWKHGNVVLFHNKLKIDFNLDHVDLLDDYSLLIRQIELEHEGLYHCWLNESIVAEFMFDVEVPPTLFMTTNAVDNLTAVNIEANTTVTFFCHAVGARPPVDLSWTIDGENRHLDVINFTVKEDGEYGVLPDLHISVNGERIANNITLLAEQLVRFVCNASASRPKSYILWYVNNMPMETFEGTNKSDCYFERDEGLYDTVSAVWYQPKEYTGSITCASHVELIGEIVEVHVKYFTYVKDISAEHQYDIWKIVCVMFVLSLIVAPLSLFIAKKLCDVTVNREKIHWDNFVKRVQDIPHHNNLVKLEGICINQDPIPVFLESRNGRPFSNISHEWPANHLELK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CQM5</accession>
    <name>A0A9Q1CQM5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_02398</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CQM5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8049593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="95" mass="10640" checksum="E7EF510DDD6C58C4" modified="2023-09-13" version="1">MGIEIPDLFPKKKSEVSVTEDSPQESSRPIPPESGVQNLEQPSPVSVDIQETDSNARKESNQSGEFYNPGTKITDEDYQEEARGIRKMKMKKKGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CRD1</accession>
    <name>A0A9Q1CRD1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_02244</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CRD1"/>
    <dbReference type="OrthoDB" id="8939918at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8049478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="139" mass="15621" checksum="21874D39FF17E621" modified="2023-09-13" version="1">MFNPGMTMHPQVTNVVRSANYHLTITFISRAGKMLSTKANKLAVHTLFTSPLDYCNGLPIGISRTLITSPKNVQHTSACNIVTRAKYESITPVLIDLHWLPIQQCIQFKALLLVYKVLHKQSPSYISRIYCSFNLLADS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CRF9</accession>
    <name>A0A9Q1CRF9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transposable element P transposase-like RNase H C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_01897</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CRF9"/>
    <dbReference type="OrthoDB" id="10063305at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048367">
        <property type="entry name" value="TNP-like_RNaseH_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21789">
        <property type="entry name" value="TNP-like_RNaseH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposable element P transposase-like RNase H C-terminal" evidence="1">
        <location>
            <begin position="170"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF21789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8049239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="229" mass="26152" checksum="D177DBC98DD2F6E5" modified="2023-09-13" version="1">MRWHPEVIKWVITVHSKSTSAYEERFEAKCTEGAKDTANIATQALLFMYNGKTIKWIHIVQLQQWDKAVHRDSPGLLLAPKLSYEHVHLSPSLQMRANVAAQWLKEDFLGFFDKWYKDVEEQPALTAKQEEAFRLSRPTMAGCHLAVNTFVDVAAYLLSKDGVKYILSDKFKQDPIEEFFSKQRAAGGYHDNPSAAQFGHHYLRNIVARRCAVGSKMSMCGAFKVMTRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CSC5</accession>
    <name>A0A9Q1CSC5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_03993</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CSC5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8050692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="57" mass="6785" checksum="B958F5C55CC9E02F" modified="2023-09-13" version="1">MPRGAEGNRLLSFARTGEREKEKNRKKEKEKTKEKKGKRRRKKKGGGWGRHIFDPYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CT78</accession>
    <name>A0A9Q1CT78_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Histidine ammonia-lyase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_03602</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PAL/histidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CT78"/>
    <dbReference type="OrthoDB" id="10051290at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.200.10">
        <property type="entry name" value="Fumarase/aspartase (Central domain)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="entry name" value="Fumarase/aspartase (N-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001106">
        <property type="entry name" value="Aromatic_Lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10362">
        <property type="entry name" value="HISTIDINE AMMONIA-LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00221">
        <property type="entry name" value="Lyase_aromatic"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="L-aspartase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8050405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="364" mass="39497" checksum="C2F5FD0A33A05050" modified="2023-09-13" version="1">MTSQLTALTYPNVFSSRLPRLLQENLIRSTLAGVGKPFSPEHTRKLLALRINVLVKGCSSVSKETLNKLVAAFNASCLPWIPEKGTVGTCGDLTPLAHLALGMMGEGKMWSPETGWGDAGEVLAKHGLQPVKLLAKEGLALINGAQLITALGAEGLQLVITEEGEIVSAGNFHGEYPAKVLDYLAIGVHELANISERRTERLINPISENKVLMHPASVDSLPTSNGQEDHVSMGGFAARKALQVVEHVEQGKLNVANRNVKFAFYKLSGLYLQTDLQVLHWLCNFSPSFNTESKVLAIELLAACQGVEFRKHLKTTAPLQAVYNLVRTVVKFGKPLGHSLEIMPPVSSMQRVPAGRVERTSRDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CTP6</accession>
    <name>A0A9Q1CTP6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_04303</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CTP6"/>
    <dbReference type="OrthoDB" id="5334309at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017868">
        <property type="entry name" value="Filamin/ABP280_repeat-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00630">
        <property type="entry name" value="Filamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50194">
        <property type="entry name" value="FILAMIN_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="Filamin" evidence="1">
        <location>
            <begin position="103"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8050920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="620" mass="69062" checksum="01C9C33D8DC07EA6" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CTV3</accession>
    <name>A0A9Q1CTV3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_03807</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CTV3"/>
    <dbReference type="OrthoDB" id="10058978at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="2">
        <location>
            <begin position="483"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="196"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="843"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="202"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="843"/>
            <end position="852"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8050559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="962" mass="108167" checksum="CDD3DA629161C35E" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H1Q4</accession>
    <name>A0A9Q1H1Q4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_29455</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H1Q4"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8029923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="62" mass="7030" checksum="77EA0F4EEBB17962" modified="2023-09-13" version="1">MLPNFQSCPKLRKTYFCSCMPTSVVVETGTSQTEAETETETGEFQNRGRDQGQNFETETETK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H253</accession>
    <name>A0A9Q1H253_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Circularly permutated Ras protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_25914</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H253"/>
    <dbReference type="OrthoDB" id="1724672at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030127">
        <property type="term" value="C:COPII vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090110">
        <property type="term" value="P:COPII-coated vesicle cargo loading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050550">
        <property type="entry name" value="SEC23_SEC24_subfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036174">
        <property type="entry name" value="Znf_Sec23_Sec24_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13803">
        <property type="entry name" value="SEC24-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13803:SF36">
        <property type="entry name" value="TYPE A VON WILLEBRAND FACTOR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82919">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="137"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="161"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8032397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="842" mass="92048" checksum="1D8216CC61B0081C" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H3Z3</accession>
    <name>A0A9Q1H3Z3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_26085</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H3Z3"/>
    <dbReference type="OrthoDB" id="10072287at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036834">
        <property type="entry name" value="Bcl-2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042398">
        <property type="entry name" value="BCL2L13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15758">
        <property type="entry name" value="BCL-2-LIKE PROTEIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15758:SF2">
        <property type="entry name" value="BCL-2-LIKE PROTEIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56854">
        <property type="entry name" value="Bcl-2 inhibitors of programmed cell death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="36"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="84"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8032534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="328" mass="36788" checksum="3ED69998FAE946FA" modified="2023-09-13" version="1">MDTPEGSQTPQDFVYKTKIVVLNYLGFGPPAFDLAPSPETLSGSETESTGSHDVISLDSDEDSNASSKPLSEEDRRESGGSLGDSGLLQVKVRSKSVSPRPSKKFSVKSPSCKSAKNVPVIPKLSSDPGRAQQKRPSLKKRHTFEMHEQYQVALRELLNQVKDTQVHGDELPNLKLPDQDWHTVYQQALRELILSVQKEDEIHYVPPRDPSQMKRDSYSSDMHDQFQKAMQELTAKMNLDGTESGDLLCPISREAIVAERIAQIGDRIMETHGKELEQALRNVIESNDGKLSYALFKAVLKETIKTHVPGWYHVSTWLLWSIYLASIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H4F6</accession>
    <name>A0A9Q1H4F6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_26069</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H4F6"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8032518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="90" mass="10066" checksum="297BA6E050DA90AD" modified="2023-09-13" version="1">MADVVESYSYEDEDEQKLLKNIPPGGVFVPLVWLRACIPQSPSPSFLPSCKHMSIYSHGAISHRSSILLYIVTTAHCRMHSLDIAYDTSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H4G3</accession>
    <name>A0A9Q1H4G3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Integrase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_25328</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H4G3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase zinc-binding" evidence="1">
        <location>
            <begin position="7"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF17921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8031951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="101" mass="11928" checksum="F99878A69F5380FA" modified="2023-09-13" version="1">MHTPLIKGTQRLKTKRFIWPNINKDVENWCQSCGKCQHLHHLRQKRRKSAKLRSQRRTRCNYGVYDCIISTLQILVSVEVSLKTHRIVQLGFPLNKKGLFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H4M5</accession>
    <name>A0A9Q1H4M5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_25823</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H4M5"/>
    <dbReference type="OrthoDB" id="8883818at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101898">
        <property type="entry name" value="NHL repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8032325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="286" mass="32855" checksum="D085FF6B207A4412" modified="2023-09-13" version="1">MASSILSFENDWYAVQCIPDICTAASNLMEDLKKDFPELATLTDDVTVNYKRYSFGKRSVTKISYQVRNNITINNPYMFVYGMTLSVDGKIVISGITSNEQSSFIIVIDMVGKLFKKRKLNTGKYSYPRYCKFSSQHKVASVCQPNETGLFDVRNGAYVKKNISDVINSWSKDRFVSCVDTDPVNNHMLVAGFNRRDVYVFDDQLKYLYILTLPETIKWPRDITVNDYHLLVCDYENEKCHLTTMDRLESKLVGEYMKPDLDGNDCTPISVCTGRAGFVYILWKTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H5X0</accession>
    <name>A0A9Q1H5X0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Microfibril-associated glycoprotein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_23789</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H5X0"/>
    <dbReference type="OrthoDB" id="6145874at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00087">
        <property type="entry name" value="FReD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050373">
        <property type="entry name" value="Fibrinogen_C-term_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040941">
        <property type="entry name" value="GGGWT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143">
        <property type="entry name" value="FIBRINOGEN/TENASCIN/ANGIOPOEITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00186">
        <property type="entry name" value="FBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="1">
        <location>
            <begin position="30"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8033526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="268" mass="31201" checksum="629F023CFC4E973B" modified="2023-09-13" version="1">MTLENGTICDVQYGAFRISDEWSDYKLSTLGTFESDADCTDVYNAGNTQDGVYYIRPAGWNDPPFKVFCNMSIDGGKWTVFQRRVNGSTNFYRNWTEYRNGFGDIKHEFWLGNEKLHFLTKQATYEYRVDFVTASLTSYHNKYNNFYVDNEDNKYRLADVGTRTGTAGYSLHGIQNTPFSTYDEDNDGRTYNCAEEHRSCWWHGADFIYVIFPFSNCRYFPSGNTTDLCSYSNLNGDYNGGNGENIYDLTSYYYCNQKYTEMKIRRIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H6K8</accession>
    <name>A0A9Q1H6K8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Rho GDP-dissociation inhibitor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_21103</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Rho GDI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H6K8"/>
    <dbReference type="OrthoDB" id="1683373at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005094">
        <property type="term" value="F:Rho GDP-dissociation inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007266">
        <property type="term" value="P:Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.50.30:FF:000003">
        <property type="entry name" value="Rho GDP-dissociation inhibitor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.50.30">
        <property type="entry name" value="Coagulation Factor XIII, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000406">
        <property type="entry name" value="Rho_GDI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024792">
        <property type="entry name" value="RhoGDI_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10980:SF3">
        <property type="entry name" value="LD16419P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10980">
        <property type="entry name" value="RHO GDP-DISSOCIATION INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02115">
        <property type="entry name" value="Rho_GDI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00492">
        <property type="entry name" value="RHOGDI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040233578" evidence="4">
        <location>
            <begin position="26"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8034320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="176" mass="20039" checksum="DDE7E83DEAC49203" modified="2023-09-13" version="1">MTNSMENFFLLPCLLLLATSSIVQSQVTPGNPTQLQDDADPRNVVVEKMIFCSEGRNDIELDLTDDLDALKDKPITIKEGCEYKLQIVFKVKREIVAGLRLFQTTYRKGVRLDKSNLMVGSYGPKAEPQVFTTAVEEAPKGMVSRGHYTMKSKFTDDDKNVHLAWEWSFDIKKDWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H7R3</accession>
    <name>A0A9Q1H7R3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">HOLleu_19339</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">Nanhai2018</strain>
            <tissue evidence="19">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8035610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="248923at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08215">
        <property type="entry name" value="STKc_Nek"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000097">
        <property type="entry name" value="Probable serine/threonine-protein kinase nek1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000262">
        <property type="entry name" value="Serine/threonine-protein kinase Nek8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058923">
        <property type="entry name" value="RCC1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051997">
        <property type="entry name" value="STK_NEK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44535">
        <property type="entry name" value="PROTEIN CBG16200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44535:SF5">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25390">
        <property type="entry name" value="WD40_RLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00633">
        <property type="entry name" value="RCCNDNSATION"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="RCC1/BLIP-II"/>
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        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
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        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
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        <property type="entry name" value="PROTEIN_KINASE_ST"/>
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    </dbReference>
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        <property type="entry name" value="RCC1_3"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12 19" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="18">
        <location>
            <begin position="298"/>
            <end position="555"/>
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    </feature>
    <feature type="repeat" description="RCC1" evidence="15">
        <location>
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    <feature type="repeat" description="RCC1" evidence="15">
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            <begin position="682"/>
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    <feature type="repeat" description="RCC1" evidence="15">
        <location>
            <begin position="734"/>
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    <feature type="repeat" description="RCC1" evidence="15">
        <location>
            <begin position="786"/>
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    <feature type="repeat" description="RCC1" evidence="15">
        <location>
            <begin position="854"/>
            <end position="905"/>
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        <location>
            <begin position="1"/>
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            <begin position="969"/>
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            <begin position="1410"/>
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            <begin position="8"/>
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            <position position="327"/>
        </location>
        <ligand>
            <name>ATP</name>
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        <source>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAJ8035610.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1442" mass="159018" checksum="D5BC281580B4DD0A" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H8J6</accession>
    <name>A0A9Q1H8J6_HOLLE</name>
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        <submittedName>
            <fullName evidence="22">Polycystic kidney disease protein 1-like 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">HOLleu_20564</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="22">Nanhai2018</strain>
            <tissue evidence="22">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the polycystin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="KAJ8036553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005261">
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Catalase core domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="4"/>
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        <property type="entry name" value="Ig-like_fold"/>
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        <property type="entry name" value="PKD/Chitinase_dom"/>
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        <property type="entry name" value="WSC_carb-bd"/>
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        <property type="entry name" value="PLAT DOMAIN-CONTAINING PROTEIN"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0969">Cilium</keyword>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="16" id="KW-0732">Signal</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040149257" evidence="16">
        <location>
            <begin position="19"/>
            <end position="4152"/>
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            <begin position="2884"/>
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        <location>
            <begin position="3602"/>
            <end position="3620"/>
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    <feature type="transmembrane region" description="Helical" evidence="15">
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    <feature type="transmembrane region" description="Helical" evidence="15">
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    </feature>
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        <location>
            <begin position="4074"/>
            <end position="4125"/>
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        <location>
            <begin position="3181"/>
            <end position="3197"/>
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    </feature>
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        <location>
            <begin position="3245"/>
            <end position="3255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
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    </evidence>
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        <source>
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        <source>
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        <source>
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    </evidence>
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        <source>
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    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KAJ8036553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="4152" mass="463175" checksum="37140E5D1662D22C" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H8K1</accession>
    <name>A0A9Q1H8K1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_20584</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H8K1"/>
    <dbReference type="OrthoDB" id="5975977at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46704">
        <property type="entry name" value="CXC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46704:SF1">
        <property type="entry name" value="TELOMERE LENGTH REGULATION PROTEIN TEL2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8036568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="335" mass="38319" checksum="A27360996C28F537" modified="2023-09-13" version="1">MDPQNTRERQTHQLRNNLLERDRHTIRVYTEPDVLIDLIRQPTPKTELKEKYAANRTFCLLQVGRIPSKFRLPDQTFVNLPTMPPWQIFDHHDSVAGNAVSADNNDINEETTDGRATTHATTVVLYQTCHFGPLPQTRMFADHSQRLKSLKTQDVSQTMLEFSAYGKKPPVTNFVRKMQQERFMCNEGLSSLICQMDLAWALVRLCPTRLFEVELIPSPTGEQQVSSWTGFNVIIHQSIQMHTTVGYCPMIHGSSTEFSTVYTLMKKIQAIMISLGQSYTVITFDLAICIKAKEIQWCQPEEFANTVIRMGGLHIILNYLSLMEKSMKGQVCKTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H900</accession>
    <name>A0A9Q1H900_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Single-stranded DNA-binding protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_14510</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H900"/>
    <dbReference type="OrthoDB" id="5600002at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008116">
        <property type="entry name" value="SSDP_DNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12610:SF12">
        <property type="entry name" value="SEQUENCE-SPECIFIC SINGLE-STRANDED DNA-BINDING PROTEIN, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12610">
        <property type="entry name" value="SINGLE STRANDED DNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04503">
        <property type="entry name" value="SSDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01743">
        <property type="entry name" value="SSDNABINDING"/>
    </dbReference>
    <dbReference type="SMART" id="SM00667">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="142"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="216"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="232"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="244"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="295"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="336"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="382"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8040272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="408" mass="43211" checksum="C5F279F49697981C" modified="2023-09-13" version="1">MFPKGKGSSVPSDSQAREKLALYVYEYLLHVGAQKTAQTFLTEIRWEKNITLGEPPGFLHSWWCVFWDLYCAAPERRDTCEHSSEAKAFHDYSAAGPNSVMGQMPPTDGMPGGPPSGPVPPGFFQPYMNPRYGGGPRPVRIPGNQPPVGVPNSQPMLPNSMDPTRPSHPSMSGPIGPRMSHPRMLTQNYPPGMRGPPPNNSMPTGLPLSLPGNRPWQPNTSTINYSQASPGSYGGPPGGGGGPPGTPIMPSPQGTPGPPGPAESRPPSARRDSTNSGDSMYSIMKPVPGGGGMPPGQNFPMGSGPDGGPMPSMAQQDMPTVMNGDMDGIPKSSPASNPPGPGPGPGPGGPGTPRDDNGEMTGQLFPPFQDNQDQNESAAILKIKETMQEEAKRFEKDTPEPHSDYFMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HA72</accession>
    <name>A0A9Q1HA72_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Alpha-N-acetylgalactosamine-specific lectin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_19157</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8038158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HA72"/>
    <dbReference type="SMR" id="A0A9Q1HA72"/>
    <dbReference type="OrthoDB" id="418245at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050111">
        <property type="entry name" value="C-type_lectin/snaclec_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22803">
        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040322668" evidence="2">
        <location>
            <begin position="18"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="3">
        <location>
            <begin position="33"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="182"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="182"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8038158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="244" mass="27858" checksum="B6994B8E6BC2C4F9" modified="2023-09-13" version="1">MEKLFLVLFVFSVTVQADEPYCNRGCPTFWTPYGRGCYRYIAEPKTWVEAEQTCRSFAGSRGLGHLVSIVTEDENSFITQLVSHSRTIVQSDHETCSGHAADPTQAKVLADEQEQEPVNADAAHIPERAGTHTIQNTLTWIGLSDIRSSGNFAWSDNSRVTYTNWLNEQNIEIFEGRDNRDYKKQKRDYEERKHEDEQQEPASGGECAHIVPVTNVLGNERSWDKWPCNDQAARSPFVCRIQWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HBT4</accession>
    <name>A0A9Q1HBT4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_10581</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HBT4"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8043482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="98" mass="10922" checksum="53501AEFF8119920" modified="2023-09-13" version="1">MVGHKSGVAAFLKEANSDIYIQGCPCHLMHLTAEKASKELLLNVEEFLIDILLSGQICKEKTGIAGISNFMWIRKQEDIKACQYQMAFLGTECKELVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HBV5</accession>
    <name>A0A9Q1HBV5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_14629</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HBV5"/>
    <dbReference type="OrthoDB" id="5989607at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009689">
        <property type="entry name" value="DUF1280"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31424">
        <property type="entry name" value="PROTEIN CBG23806"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31424:SF3">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06918">
        <property type="entry name" value="DUF1280"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="285"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8040364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="348" mass="39738" checksum="66026DADAF750ADB" modified="2023-09-13" version="1">MLYDMNLTRVTLPKGEFLTVKADICNSWKKVRELKRLLKQFGIASESEASLRAQQAYIINDNINAQWLPLVYKDEDGGTIIKRTPWVSVKSLEDKVFQQLDGLNSLNYLKWNDAIPQDEIWRKIGGDKGGGNFKQVFQIGNVEHPNSPHNTIVTTIFQVDGSLHNLKVGLHCCLWCNITKEGMKIPLKERGRSENRTLEKLQNFNHMFVQIGAKLSEAKNFYNVIADALFNIPIDQVCPPGVHLSLGLYLKHFASFENQCHLLDVKMAKTLHHPTPESSKDFPSTTAAYTKARQLEEEANSLDEEANILIEHITNIQEEDQAQIYHSTIKQHLEERDKVVSNKYTVVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HEH5</accession>
    <name>A0A9Q1HEH5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_10378</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HEH5"/>
    <dbReference type="OrthoDB" id="1902038at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35385:SF2">
        <property type="entry name" value="PROTEIN B, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35385">
        <property type="entry name" value="PROTEIN B, PUTATIVE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8043334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="254" mass="29376" checksum="3594645A9B2AD414" modified="2023-09-13" version="1">MKGNEPRHHLRWKKLSLQSAPKLRSHRHNPTVGTAFNHKFSDIYLGKPTAVPPFAIRVRKALITGVYQPKFRGTAYQLGATAVERRDMSLALLPKLHEDQSYRVYDYKEDGNTVQCTLRLPLTTKDDALKWLKNFQETSFSTWRVDKTYPHAGNATGNKAKNIFRDVHIREGFPTIINLIWEHNHEVECADAFRKRDVNDTTKEKLLELFRAGHSPSSALDTLKYDLQSDNTNEEYLRLSADKSICPDLQYCYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HF39</accession>
    <name>A0A9Q1HF39_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Lissencephaly-1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_11344</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus end. Required for several dynein- and microtubule-dependent processes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
            <location evidence="11">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
            <location evidence="11">Cytoskeleton</location>
            <location evidence="11">Microtubule organizing center</location>
            <location evidence="11">Centrosome</location>
        </subcellularLocation>
        <text evidence="11">Localizes to the plus end of microtubules and to the centrosome.</text>
    </comment>
    <comment type="domain">
        <text evidence="11">Dimerization mediated by the LisH domain may be required to activate dynein.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the WD repeat LIS1/nudF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8044009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HF39"/>
    <dbReference type="OrthoDB" id="674604at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005875">
        <property type="term" value="C:microtubule associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070840">
        <property type="term" value="F:dynein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000132">
        <property type="term" value="P:establishment of mitotic spindle orientation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051012">
        <property type="term" value="P:microtubule sliding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000038">
        <property type="entry name" value="Lissencephaly-1 homolog B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.960.30:FF:000002">
        <property type="entry name" value="Platelet-activating factor acetylhydrolase ib"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.960.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03141">
        <property type="entry name" value="lis1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017252">
        <property type="entry name" value="Dynein_regulator_LIS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037190">
        <property type="entry name" value="LIS1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056795">
        <property type="entry name" value="PAC1-like_LisH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050349">
        <property type="entry name" value="WD_LIS1/nudF_dynein_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44129">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN POP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25173">
        <property type="entry name" value="Beta-prop_WDR3_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24951">
        <property type="entry name" value="LisH_PAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037647">
        <property type="entry name" value="Dynein_regulator_Lis1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00667">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109925">
        <property type="entry name" value="Lissencephaly-1 protein (Lis-1, PAF-AH alpha) N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4 11" id="KW-0132">Cell division</keyword>
    <keyword evidence="8 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5 11" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7 11" id="KW-0498">Mitosis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 11" id="KW-0813">Transport</keyword>
    <keyword evidence="3 12" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="PAC1-like LisH-like dimerisation" evidence="13">
        <location>
            <begin position="5"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="12">
        <location>
            <begin position="102"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="12">
        <location>
            <begin position="144"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="12">
        <location>
            <begin position="186"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="12">
        <location>
            <begin position="228"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="12">
        <location>
            <begin position="314"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="12">
        <location>
            <begin position="341"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="12">
        <location>
            <begin position="383"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF24951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ8044009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="417" mass="47545" checksum="62E3969786EEE999" modified="2023-09-13" version="1">MVLSQRQKEELNKAIADYLRSNGFENSLEEFTKEAKIDGDLEKKHAGLLEKKWTSVIRLQKKVMDLESKLNETEREFQSGGPTRERRSAKEWIPRPPEKYCLTGHRSPITRVAFHPVYSLMVSSSEDATIKVWDYETGDFERTLKGHTNAVQDISFDHTGKYLASCSADMSIRLWDFQTYECIKTLQGHDHNVSSVNFLPSGDYLVSCSRDKTIKLWEVATGYCTKTFQGHREWVRMVKPNPDGSLLASASNDQTVRVWVVATKECKLELREHDHVVECVAWAPESAHSTLNEATGAEITNNMDGLEVKKGARSGPFLVSGSRDKTVKIWDISTEVCIMTLHGHDNWVRGVLFHPGGRYIISAADDKTLRIWDYKNKRSMKTLEAHEHFVTCLDFHRSAPYVISGSVDLTIKVWECR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HGW5</accession>
    <name>A0A9Q1HGW5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_08356</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HGW5"/>
    <dbReference type="OrthoDB" id="10551871at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8045360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="150" mass="17323" checksum="E9298B2752EDC80D" modified="2023-09-13" version="1">MNGKVSKYLKRTNERLVASVHFSKEKTDSVESLVVEDDLADDEEIETAPKDTRVDIVIDDVSNQNDIDAVNVEDEQEVKKPKRTIWTFFKWLWAHLRLRKWAGRGLQAFGQGFMYTSPMSATVVIAQEFDKRHRSYNGGSSSKYHQTHVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HJS2</accession>
    <name>A0A9Q1HJS2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_02467</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HJS2"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8049634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="73" mass="8709" checksum="B79A24B41F632DBD" modified="2023-09-13" version="1">MQADQLTGRERGRSKFREYVEKRKENEDEMRKRVNRPKREAAEKKKQKEAEKKNNNDKPVSSSRSDDQDELNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HJS7</accession>
    <name>A0A9Q1HJS7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AP-5 complex subunit beta-1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_06759</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HJS7"/>
    <dbReference type="OrthoDB" id="646197at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048978">
        <property type="entry name" value="AP5B1_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21587">
        <property type="entry name" value="AP5B1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="AP-5 complex subunit beta-1 N-terminal" evidence="2">
        <location>
            <begin position="40"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF21587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8047701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="126" mass="14086" checksum="8A985E0F5A8FBC6E" modified="2023-09-13" version="1">MSLKNIAEDSHWQQVSVAMKRDLQEFTHKKGSSEDECVLDLLQALYTEGITDATKVMMLGVLQEMSESLFMDETSIEQAIGTLIGFYQEVTMNKGSTFLKSQILVCLTTLALTGNQLVSVLHLLWP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HKP9</accession>
    <name>A0A9Q1HKP9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Beta-lactamase domain-containing protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_02171</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HKP9"/>
    <dbReference type="OrthoDB" id="5946976at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="entry name" value="DD-peptidase/beta-lactamase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001466">
        <property type="entry name" value="Beta-lactam-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052907">
        <property type="entry name" value="Beta-lactamase/esterase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43319">
        <property type="entry name" value="BETA-LACTAMASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43319:SF3">
        <property type="entry name" value="BETA-LACTAMASE-RELATED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00144">
        <property type="entry name" value="Beta-lactamase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="beta-lactamase/transpeptidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Beta-lactamase-related" evidence="2">
        <location>
            <begin position="51"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="12"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8049425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="432" mass="48543" checksum="215B60BAE0F46307" modified="2023-09-13" version="1">MLLLRKPGKWSAERQASQRGRLKLSSQHGIDGRRLPRNRKNYEDNWDNPEAGSAFSVYYKGEKVVDLWAGYADVEAKRLWKEDTMTVIFSTSKGLVALCAAMLVDRSYLDFKKTVAHYWPEFAQQGKENITVEQLLEHEAGLVGTNSPLSYALLEDHAALDKVLAETRPKWKPGERHGYHAFTFGPYVDALLRRVDPKKRTIGQFFDEEVSKPFGIDAYIGAPLEISHRIGRSSNLKLTKRLSHIIYAIRNIGMFQRYLLGRLFGGGETEEVLNVMFQSCGDICEIPRLGDPDVRRIEFASGNAVATARAVAKLFGILANGGKQNNKTLLSKTIIDEYVHDKRGPTPDLVMFDHPIRWKYGMDVIPQGKNAGNLFGSPGIGGQIGYADPNNNLGYGFISRHMSPMGIQMLDPRFKNLQESVLDVIGRNKEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HL41</accession>
    <name>A0A9Q1HL41_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_01806</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HL41"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8049183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="72" mass="8131" checksum="5C993FD0A88F3821" modified="2023-09-13" version="1">MESSREFNFNAAFLELSRKIVIIHHRLALSLFARGTSSYAATARCRSTTIPSSTCMHYQPLDQTRQILSKGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HL78</accession>
    <name>A0A9Q1HL78_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_02196</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HL78"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8049441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="96" mass="11111" checksum="8FFE7EEF083F2E3B" modified="2023-09-13" version="1">MTAPGSLSVRKVPNSSPTLVELKKHHHDQRLVSFNCEDFPRLCIAKGGKNFPQKSKIFARLTRRCTSQTDTFKLSVVSLRYHPLSFPQTVIIWWHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HLG2</accession>
    <name>A0A9Q1HLG2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Arylsulfatase G</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_03655</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sulfatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HLG2"/>
    <dbReference type="OrthoDB" id="103349at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004065">
        <property type="term" value="F:arylsulfatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050738">
        <property type="entry name" value="Sulfatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024607">
        <property type="entry name" value="Sulfatase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000917">
        <property type="entry name" value="Sulfatase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42693">
        <property type="entry name" value="ARYLSULFATASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42693:SF42">
        <property type="entry name" value="ARYLSULFATASE G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00884">
        <property type="entry name" value="Sulfatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14707">
        <property type="entry name" value="Sulfatase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00149">
        <property type="entry name" value="SULFATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Sulfatase N-terminal" evidence="7">
        <location>
            <begin position="68"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8050445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="536" mass="58412" checksum="3E088F73A95E4571" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0Y9J0</accession>
    <name>A0A9Q0Y9J0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Zinc finger PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_43517</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000341">
        <property type="protein sequence ID" value="KAJ8018478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0Y9J0"/>
    <dbReference type="OrthoDB" id="775972at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041373">
        <property type="entry name" value="RT_RNaseH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF1">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17917">
        <property type="entry name" value="RT_RNaseH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Zinc finger PHD-type" evidence="10">
        <location>
            <begin position="246"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8018478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="322" mass="37606" checksum="8AFCAE6A1A937419" modified="2023-09-13" version="1">MLAEYIKQLCEENHLDLTLLKTKEDRIQALQMLPNLRDAGLNLNNSSREKPVETFNVKLKGFHVLTSDDDVECYLSTFERLCIKHNIPEANWTIILESFLTGKAQRAYFAWTENEKEDYQLVKESVLYAYQLTPSTYREKFRNASKNSSETFRQFSSRLFLYLRRWLNPSDDLLSSKGSQITVFTDHKALVWMLRNKALKGKLVRWALRLQEFDYTVHYRPGIQNMIPDALSRAPQICSIDYDSNVCFHEDCKGDPSNVVNWVQCDSCDKWYHCRCLKITKEEADDLDPYVCVKCELEVEKPESAQALKENCKVQSVLPDRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YBV5</accession>
    <name>A0A9Q0YBV5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_42156</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000036">
        <property type="protein sequence ID" value="KAJ8019315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YBV5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8019315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="89" mass="10538" checksum="EB06A2CAB3530427" modified="2023-09-13" version="1">MDRVAYGYLKQNCDGCDIEWPSQLDHRCTNFGSNIAMSDIYFNHLQYLVKGEWVINATRNYLLNGVEISTVRAVMQQLRTIWSQYRWMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YBZ5</accession>
    <name>A0A9Q0YBZ5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Vacuolar protein sorting-associated protein 8-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_44912</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010001443">
        <property type="protein sequence ID" value="KAJ8017579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YBZ5"/>
    <dbReference type="OrthoDB" id="289913at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23410">
        <property type="entry name" value="Beta-prop_VPS8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8017579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="113" mass="12774" checksum="85CD8248E0EF5A5E" modified="2023-09-13" version="1">MFYFTSCGEVFTLALLRTNFLIKDHPLKDMMLLAMATLSKVFMVHLRPGLTVIFTKQLKGPATTLPLLAWQIVIIQVTDSSRVIDPVLAFGRHDTIYFYLVPFVVPCITLSLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YFY2</accession>
    <name>A0A9Q0YFY2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">D-2-hydroxyglutarate dehydrogenase, mitochondrial</fullName>
            <ecNumber evidence="11">1.1.99.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HOLleu_38377</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Nanhai2018</strain>
            <tissue evidence="16">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes the oxidation of D-2-hydroxyglutarate (D-2-HG) to alpha-ketoglutarate. Also catalyzes the oxidation of other D-2-hydroxyacids, such as D-malate (D-MAL) and D-lactate (D-LAC). Exhibits high activities towards D-2-HG and D-MAL but a very weak activity towards D-LAC.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>(R)-malate + A = oxaloacetate + AH2</text>
            <dbReference type="Rhea" id="RHEA:67460"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15588"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:67461"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FAD-binding oxidoreductase/transferase type 4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.99.39" evidence="11"/>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YFY2"/>
    <dbReference type="OrthoDB" id="5332616at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051990">
        <property type="term" value="F:(R)-2-hydroxyglutarate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006108">
        <property type="term" value="P:malate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2190:FF:000001">
        <property type="entry name" value="D-2-hydroxyglutarate dehydrogenase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2740:FF:000002">
        <property type="entry name" value="D-2-hydroxyglutarate dehydrogenase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.43.10:FF:000002">
        <property type="entry name" value="D-2-hydroxyglutarate dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.465.10:FF:000053">
        <property type="entry name" value="D-lactate dehydrogenase (Cytochrome), putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.45.10:FF:000001">
        <property type="entry name" value="D-lactate dehydrogenase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.10">
        <property type="entry name" value="Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.45.10">
        <property type="entry name" value="Vanillyl-alcohol Oxidase, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004113">
        <property type="entry name" value="FAD-bd_oxidored_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016167">
        <property type="entry name" value="FAD-bd_PCMH_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016164">
        <property type="entry name" value="FAD-linked_Oxase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051264">
        <property type="entry name" value="FAD-oxidored/transferase_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016171">
        <property type="entry name" value="Vanillyl_alc_oxidase_C-sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43716">
        <property type="entry name" value="D-2-HYDROXYGLUTARATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43716:SF1">
        <property type="entry name" value="D-2-HYDROXYGLUTARATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02913">
        <property type="entry name" value="FAD-oxidase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55103">
        <property type="entry name" value="FAD-linked oxidases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="15">
        <location>
            <begin position="145"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00039639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ8021234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="573" mass="62706" checksum="178CB81DE88458A4" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YG41</accession>
    <name>A0A9Q0YG41_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_44205</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000707">
        <property type="protein sequence ID" value="KAJ8018031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YG41"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8018031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="63" mass="6986" checksum="C16E810076013AAC" modified="2023-09-13" version="1">MFICYTGMKDHIVSPVIVMSPSVTFSSTAMNTPYSSEVLHCATPEDIVRAHRSVSHFGLSQRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YII5</accession>
    <name>A0A9Q0YII5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Coiled-coil-helix-coiled-coil-helix domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_37768</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YII5"/>
    <dbReference type="OrthoDB" id="5825849at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005761">
        <property type="term" value="C:mitochondrial ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1130">
        <property type="entry name" value="CytochromE C oxidase copper chaperone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010625">
        <property type="entry name" value="CHCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009069">
        <property type="entry name" value="Cys_alpha_HP_mot_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033620">
        <property type="entry name" value="Ribosomal_mS37_met"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31278">
        <property type="entry name" value="CHCHD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31278:SF2">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06747">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47072">
        <property type="entry name" value="Cysteine alpha-hairpin motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51808">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CHCH" evidence="3">
        <location>
            <begin position="44"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="88"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="96"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF06747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8022779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="116" mass="13228" checksum="D81D79E9C1D1A3AA" modified="2023-09-13" version="1">MAKRASEIVIRNTFKPEKYTNMNKPFKLRDRVSLRRTKQGAASCISEMAVLMSCWKENDYSDKKCSKELETFNKCVAAAAAERKAAVKAMKEETSHGSQMTSKQVTRMLQKYPQPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YIU5</accession>
    <name>A0A9Q0YIU5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_35706</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YIU5"/>
    <dbReference type="OrthoDB" id="775972at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33244">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33244:SF3">
        <property type="entry name" value="PEPTIDASE A2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="73"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8023320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="126" mass="14647" checksum="D63ACCEBD3CFFC66" modified="2023-09-13" version="1">MKGHHDTKVKVKDLPPLYPGQKVRILDYERKTWSPGEVIKVCQEPRSYDIRTPNGSILRRNRQHLRELRHAIPQPHHTTPQPQHTSPQPHSTPQVKYPSNLTLPMVTTCRHLQKFGLQMNTKEKGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YJL7</accession>
    <name>A0A9Q0YJL7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Reticulocalbin-3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_36323</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Probable molecular chaperone assisting protein biosynthesis and transport in the endoplasmic reticulum. Required for the proper biosynthesis and transport of pulmonary surfactant-associated protein A/SP-A, pulmonary surfactant-associated protein D/SP-D and the lipid transporter ABCA3. By regulating both the proper expression and the degradation through the endoplasmic reticulum-associated protein degradation pathway of these proteins plays a crucial role in pulmonary surfactant homeostasis. Has an anti-fibrotic activity by negatively regulating the secretion of type I and type III collagens. This calcium-binding protein also transiently associates with immature PCSK6 and regulates its secretion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Interacts with PCSK6 (immature form including the propeptide); probably involved in the maturation and the secretion of PCSK6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YJL7"/>
    <dbReference type="OrthoDB" id="293868at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd16226">
        <property type="entry name" value="EFh_CREC_Calumenin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000104">
        <property type="entry name" value="calumenin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10827:SF52">
        <property type="entry name" value="IP16409P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10827">
        <property type="entry name" value="RETICULOCALBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13202">
        <property type="entry name" value="EF-hand_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040234772" description="Reticulocalbin-3" evidence="12">
        <location>
            <begin position="28"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="13">
        <location>
            <begin position="79"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="13">
        <location>
            <begin position="163"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00056975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00063143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00072696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ8023785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="329" mass="38256" checksum="8A413D212E42EB71" modified="2023-09-13" version="1">MHEVKLSIHKIFVFVTTLCLTTVFANAANVHGGERVHEPEKLSNQAHFNSHNQHNAVYDHDAFLGADEARKFEHLSPEESRKRLGKIVDKIDTNRDGFVSEAELLEEIKHHQNRYAYKDADRLWKAQNTNKDSVLTWAEYNETAYGGQTEEQLLKIETQDFKKKLRRDRHRWAMADQDRDRNLSYEEFAAFLHPEAFIHMQDVVAEETLEDMDDDKDGFVSLEEHIDDLWAREGPNAPEPEWVPREREYFMVSRDRDGDGKLNAGELREWIFPSNHDPAEAEMKHLLYEADKNKVDGKLTKTEIVDKFDLFAGSQATDFGDAIKAHDEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YK44</accession>
    <name>A0A9Q0YK44_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_37891</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YK44"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8022871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="50" mass="5960" checksum="66C7A172565BF614" modified="2023-09-13" version="1">MCDVICDVGFFNSLRDAEKIYDTMYIYEEWLQYTGVFIAKKVQSLKRSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YKA5</accession>
    <name>A0A9Q0YKA5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">BTB/POZ domain-containing protein KCTD21</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_39054</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YKA5"/>
    <dbReference type="OrthoDB" id="2414723at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14499:SF144">
        <property type="entry name" value="POTASSIUM CHANNEL TETRAMERISATION-TYPE BTB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14499">
        <property type="entry name" value="POTASSIUM CHANNEL TETRAMERIZATION DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="1">
        <location>
            <begin position="6"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8021762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="210" mass="23516" checksum="2754DD037A16520E" modified="2023-09-13" version="1">MADDDSYMILNVGGICYTTSRLTLTSCPDSMLGIMFSEGGVPPKADKNGHFFIDRDGAMFRYILNFIRDGRLCLPSSFEEYDQLLAEAEFFQLSALKEEVLSRMNPQNASEVNETNTGKEVVILKNIHPWSTDTLYVVEATKQTWPRLKSLASVQSVELPPYAASNKVCFDNDIKGYRADCCQLVALVIKDGFKLETAVPPSEKNFLIFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YKK9</accession>
    <name>A0A9Q0YKK9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_40058</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YKK9"/>
    <dbReference type="OrthoDB" id="6502630at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8020461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="81" mass="8993" checksum="DB8CE1E8C5FF1E2C" modified="2023-09-13" version="1">MRYGKGKNPIVFDGVQRSSGVTGGRTLKTLLTCYLKMHYGKRKNPIIFGGGQRSSGVTRGQILKTLLTQYLKMGSLDKFHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YM12</accession>
    <name>A0A9Q0YM12_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Sialidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_34989</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YM12"/>
    <dbReference type="OrthoDB" id="8452296at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006207">
        <property type="entry name" value="Cys_knot_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006208">
        <property type="entry name" value="Glyco_hormone_CN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051836">
        <property type="entry name" value="Kremen_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001007">
        <property type="entry name" value="VWF_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24269">
        <property type="entry name" value="KREMEN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24269:SF16">
        <property type="entry name" value="PROTEIN SLG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00007">
        <property type="entry name" value="Cys_knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57603">
        <property type="entry name" value="FnI-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CTCK_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CTCK_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50184">
        <property type="entry name" value="VWFC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="VWFC" evidence="7">
        <location>
            <begin position="846"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="domain" description="CTCK" evidence="6">
        <location>
            <begin position="910"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="45"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="124"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="553"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="53"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="130"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="554"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="585"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="746"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="910"/>
            <end position="960"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="925"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="936"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="940"/>
            <end position="992"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS01225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8024928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1013" mass="108604" checksum="B42F148BB810164B" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YNJ8</accession>
    <name>A0A9Q0YNJ8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Dysferlin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HOLleu_33409</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Nanhai2018</strain>
            <tissue evidence="17">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ferlin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10059618at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007009">
        <property type="term" value="P:plasma membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08373">
        <property type="entry name" value="C2A_Ferlin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04011">
        <property type="entry name" value="C2B_Ferlin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04018">
        <property type="entry name" value="C2C_Ferlin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04017">
        <property type="entry name" value="C2D_Ferlin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04037">
        <property type="entry name" value="C2E_Ferlin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08374">
        <property type="entry name" value="C2F_Ferlin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000026">
        <property type="entry name" value="dysferlin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
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        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037726">
        <property type="entry name" value="C2A_Ferlin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037720">
        <property type="entry name" value="C2B_Ferlin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037722">
        <property type="entry name" value="C2C_Ferlin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037723">
        <property type="entry name" value="C2D_Ferlin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037724">
        <property type="entry name" value="C2E_Ferlin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037725">
        <property type="entry name" value="C2F_Ferlin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012968">
        <property type="entry name" value="FerIin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037721">
        <property type="entry name" value="Ferlin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012560">
        <property type="entry name" value="Ferlin_A-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012561">
        <property type="entry name" value="Ferlin_B-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032362">
        <property type="entry name" value="Ferlin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055072">
        <property type="entry name" value="Ferlin_DSRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006614">
        <property type="entry name" value="Peroxin/Ferlin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12546">
        <property type="entry name" value="FER-1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12546:SF33">
        <property type="entry name" value="SPERM VESICLE FUSION PROTEIN FER-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22901">
        <property type="entry name" value="dsrm_Ferlin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08165">
        <property type="entry name" value="FerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08150">
        <property type="entry name" value="FerB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08151">
        <property type="entry name" value="FerI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16165">
        <property type="entry name" value="Ferlin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00694">
        <property type="entry name" value="DysFC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00693">
        <property type="entry name" value="DysFN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01200">
        <property type="entry name" value="FerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01201">
        <property type="entry name" value="FerB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01202">
        <property type="entry name" value="FerI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="2164"/>
            <end position="2184"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="16">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="16">
        <location>
            <begin position="183"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="16">
        <location>
            <begin position="337"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="16">
        <location>
            <begin position="1166"/>
            <end position="1292"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="16">
        <location>
            <begin position="1327"/>
            <end position="1451"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="16">
        <location>
            <begin position="1677"/>
            <end position="1795"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="16">
        <location>
            <begin position="1911"/>
            <end position="2060"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="141"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="461"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1131"/>
            <end position="1166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1444"/>
            <end position="1478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1513"/>
            <end position="1585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="151"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="182"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="467"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1562"/>
            <end position="1581"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ8025764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="2200" mass="247530" checksum="756F10994C1AA02D" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YQU1</accession>
    <name>A0A9Q0YQU1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_31847</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YQU1"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8026887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="190" mass="21149" checksum="7DF92381F6B5E659" modified="2023-09-13" version="1">MRYGMRKNPIIFGGGERSSGVTGGHSLKTLLTRYLKMPYIKRKNPFVFGGGQRSPGVTGGQTLKTLLTQYLEMRYGKRKNPIIFGGGERSSGVTGDQTLKILLTQYLEMGYGKRKSPISFGGGQRSFGVTRGQTLKTLLTCYLQMCYSKRKNPIIFGGGQRSSGVTRGQCLKTLSRRYLQVGSLNRFHTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YR13</accession>
    <name>A0A9Q0YR13_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uronyl 2-sulfotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_34730</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sulfotransferase 3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YR13"/>
    <dbReference type="OrthoDB" id="10019582at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007734">
        <property type="entry name" value="Heparan_SO4_2-O-STrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005331">
        <property type="entry name" value="Sulfotransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12129">
        <property type="entry name" value="HEPARAN SULFATE 2-O-SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12129:SF15">
        <property type="entry name" value="URONYL 2-SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03567">
        <property type="entry name" value="Sulfotransfer_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8024732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="336" mass="39444" checksum="0AA80B8024E233E1" modified="2023-09-13" version="1">MTKSKHRYWTLVFMVPTVLLIVVAWNSQWVETYVCTTSGQCNKHKDIYFRRLHHWKEIYFSNTEHSPSKSLSSSFGHVRNETDLIIVFFAVPKTGSRSVSNPFNMLYTSTDRMYQIVPAEDRVSSESLTSYLTKMPPATFVRGHFPIVNLPRNYVPISVLRDPLERFISEFNFVKHGDGQIGTERIKIHDQPEFELSIDECVRRKGLFCSRESVATYTLKFFCGYDPKCAVANEWTYKQAVDNVEDKFLMVGITEDLNSTMELIEILLPNMSKGVVSIYHEKQKLKRGTYITHRTENPSPRIRARLRKMMYWEYKFYHHVKMKFSSLKKQFGLPER</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YRT3</accession>
    <name>A0A9Q0YRT3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CUB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_36919</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8024241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YRT3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8024241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="124" mass="14533" checksum="05533A7C025D442C" modified="2023-09-13" version="1">MGTFRRREICQLEHTFAWTSDFNGMFLHLQSTQQELPTMTLEWESVDRSVPDECITVVTDENTTLLIMGIPSIRQQPSTCQIIVNFRPDRRVVLSFESFQMTSSNCSSYITVSYSQLYKLRLHW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YS29</accession>
    <name>A0A9Q0YS29_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_32972</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YS29"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8025422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="64" mass="7368" checksum="E260430EB5EA270D" modified="2023-09-13" version="1">MGNNTGVPLKEISMQARDQIIRHGRRHFNLCIYHNRDVLLINSDIIPTCHVMKYSIFNSALTSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YU35</accession>
    <name>A0A9Q0YU35_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Pancreatic lipase-related protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_34102</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8026296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YU35"/>
    <dbReference type="OrthoDB" id="199913at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016298">
        <property type="term" value="F:lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00707">
        <property type="entry name" value="Pancreat_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013818">
        <property type="entry name" value="Lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033906">
        <property type="entry name" value="Lipase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000734">
        <property type="entry name" value="TAG_lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610">
        <property type="entry name" value="LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610:SF178">
        <property type="entry name" value="LIPASE MEMBER H-A-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00151">
        <property type="entry name" value="Lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00821">
        <property type="entry name" value="TAGLIPASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040437977" evidence="5">
        <location>
            <begin position="26"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="Lipase" evidence="6">
        <location>
            <begin position="46"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8026296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="292" mass="32900" checksum="32BEF01FD0B58DEC" modified="2023-09-13" version="1">MVGHFDMCTILTIAFVFFCTTNVAARKVCYEGLDCFDTSLPCHVVPLPEKPSKINTEFYLFTPENPTTHEVIDWRKDGSIVSSKFQSSRDTKFIIHGYTENILLPQYVKMKDALLSIGKFNVILVNWKDGAEVDRDDLLRNLLKLPKDYLQARQNTRLVGRQIGELSRKLQKLKKASFSSMHLIGFSLGAHVAGYAGEHVSGQYGRITGLDPAGPLYRSFTPWKGCLLEKSDAVFVDIIHTDDVLGNQKAVGHQDFYPSGGKHQVSCPFLDFICDHERAVEFFTESINPKCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1B8Q2</accession>
    <name>A0A9Q1B8Q2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_44308</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000779">
        <property type="protein sequence ID" value="KAJ8017961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1B8Q2"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8017961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="213" mass="24056" checksum="754E32149A9D331A" modified="2023-09-13" version="1">MDENPSEKGSHSRKRPRERKSTNICLHKTYEEWAHQKEKYEEHRKAFRLSSGMSSRDFRDTATHGDFAKHLLESHSRFCEFCRKNANLTPTETFPIPDQPPASYTVFTDACTQTETESPDDDATADSSQGHVHLRQGIPSGVQNAALQKLHNTASAANWTVNTRYIYVNIFSKLGAQRVHHLYSSFTILSLGSVVQVNKCSTCALFDNGTSFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BBP2</accession>
    <name>A0A9Q1BBP2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_41188</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8019548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BBP2"/>
    <dbReference type="OrthoDB" id="10261408at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="5"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="103"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8019548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="156" mass="17191" checksum="0FAA341A07B55E32" modified="2023-09-13" version="1">MSHLCHICQLPFTRGYNLGRHIERVHKHGHGDLEMYGGGSKKGEGKRHHDSSDEGSYDDISESDTNDSSSEPLEEDDMTDSSETDDSNAESDTEGADSSKEESTEDDVVTDNDGEDDDGSENVCCPIDTWEALAHINKAMGVILKRELKKWTDSAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BBP8</accession>
    <name>A0A9Q1BBP8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_42238</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000045">
        <property type="protein sequence ID" value="KAJ8019264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BBP8"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="34"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8019264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="90" mass="9807" checksum="7D3905FB9400A36D" modified="2023-09-13" version="1">MPSAPLSPSCWYAPLAKRPHHPVHSHFISRNPDIATAGFVLLFVLLFISGAVAEWIKAVAFEAMKLGNQEQRAGRHPAGRFGVRSTDGSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BDM3</accession>
    <name>A0A9Q1BDM3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Urease accessory protein ureG-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_38802</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SIMIBI class G3E GTPase family. UreG subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BDM3"/>
    <dbReference type="OrthoDB" id="10063137at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043419">
        <property type="term" value="P:urea catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05540">
        <property type="entry name" value="UreG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000208">
        <property type="entry name" value="Urease accessory protein UreG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01389">
        <property type="entry name" value="UreG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003495">
        <property type="entry name" value="CobW/HypB/UreG_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004400">
        <property type="entry name" value="UreG"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00101">
        <property type="entry name" value="ureG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31715">
        <property type="entry name" value="UREASE ACCESSORY PROTEIN G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31715:SF0">
        <property type="entry name" value="UREASE ACCESSORY PROTEIN G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02492">
        <property type="entry name" value="cobW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0996">Nickel insertion</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CobW/HypB/UreG nucleotide-binding" evidence="7">
        <location>
            <begin position="64"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="14"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="25"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8021558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="259" mass="28430" checksum="253A8AEC0D584E10" modified="2023-09-13" version="1">MAESNVSFKKEGAFAEAKGGHSHSHSHDHGHEHGHSHEAMENPGTFLDRDMPITRTDWSERAFTVGIGGPVGSGKTALVLSLCKHLREKIGICVVTNDIFTREDWEFLVRNKALPEERMRAVETGGCPHAAIREDYSVNMETIRDLTKEFSPQIILVESGGDNLAANFSRELADYIIYVIDVAGGDKIPRKGGPGITQSDLLVINKTDIAEAVGASLEVMKRDSDLMRQKGPTIFAQVKHMVGVPEIADLILQSYKSKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BGS7</accession>
    <name>A0A9Q1BGS7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_35281</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8025158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BGS7"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8025158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="52" mass="5501" checksum="5373327CB3B79EE5" modified="2023-09-13" version="1">MSLIGDIIVHSFLFWSDKPSSSISLTVPTKSEGGTAGQHSSSSVLTVVQFYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BGT9</accession>
    <name>A0A9Q1BGT9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Tyrosine-protein kinase ABL1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_34591</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BGT9"/>
    <dbReference type="OrthoDB" id="535945at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004714">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00192">
        <property type="entry name" value="PTKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001346">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050122">
        <property type="entry name" value="RTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416:SF620">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR TORSO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="322"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="322"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8024634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="354" mass="40036" checksum="DD0126A429390E62" modified="2023-09-13" version="1">MIDIGKHPHIMPLYGCCTITEPYYLITEFMKYGALDKFLRRCRSMENATIDPIYNLKELNQVQIAWQISKGMAFLSTTKFFHGDLAARNCLVGADLVVKISDFGLSDDVYQSGYKRIAPEKKRPVKWWSYGIVMWEIYSLGDIPYPGMPATEVVKRILKGYRLPQPDGCPLDIYKIMLECWQQSPKERPTFSELEYALELILHHRADKDIRVTTGELHENPYLPVNDQSEIPSAVRKDSSLAQKINLSELFGTSADSAIYDMNNTSTASFIVDNRLVGAPISVQPIFEETLLPSDKGNSTGSFDFDPELLSSDSDSFVFTDIIDEESEDGENEDETAVKRDIDIAQGKKQAQKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BHK3</accession>
    <name>A0A9Q1BHK3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_31883</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BHK3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8026915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="152" mass="17152" checksum="9166864225627397" modified="2023-09-13" version="1">MTIPEEELLIGKWFYLSHMVYQVEILFVEAKSLKAQRQQDFVVMFLYNRTAIQQNKLFTEILCELITPEDCSEDSENSESPTSPEDGRGPLLDENASFTIDPKTALDSPQSCPESGAVEAGYRKNLYAALWLLSFICHLGFAGTGTSHLNFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BHM3</accession>
    <name>A0A9Q1BHM3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA-directed DNA polymerase</fullName>
            <ecNumber evidence="2">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_31988</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="2"/>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BHM3"/>
    <dbReference type="OrthoDB" id="5871067at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004868">
        <property type="entry name" value="DNA-dir_DNA_pol_B_mt/vir"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33568">
        <property type="entry name" value="DNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33568:SF3">
        <property type="entry name" value="DNA-DIRECTED DNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03175">
        <property type="entry name" value="DNA_pol_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0235">DNA replication</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA-directed DNA polymerase family B mitochondria/virus" evidence="9">
        <location>
            <begin position="44"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8026990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="350" mass="40469" checksum="668CA817022F39BD" modified="2023-09-13" version="1">MVCDLCIMEPFESECLVCEEKQVILQGPNTKREFCEWLLAPPRNNSTCIAHNLKGFDGYFILQHLYDNGVVPPIITNGAKVMSIKLLRNSTRFIDSVNFLRMPLSNMPKTFGFNELKKGYFPHLFKFNTTENQTYIGHFPEASYYAPDVMSSEKRKDFFKWYETEKNKGLLFDFQKELGAYCISDVDILRRCCLKFRSLFMDTTCKEIDNNVEDEAEEGDGVVTEMCGVDPFKHCITIASACNLVFRRNYMKPNSIAVFTNDKPKSYSFAALEWLYYESKQRGVYIQHAQNEGEEKIGNYRVDGFAKEGKIIFSFQGCFWHGCLKCFNEDTMHPAKNESMGEVFKRSEKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BI73</accession>
    <name>A0A9Q1BI73_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Polyphosphoinositide phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_31327</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BI73"/>
    <dbReference type="OrthoDB" id="405996at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043813">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043573">
        <property type="entry name" value="Fig4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002013">
        <property type="entry name" value="SAC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45738">
        <property type="entry name" value="POLYPHOSPHOINOSITIDE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45738:SF5">
        <property type="entry name" value="POLYPHOSPHOINOSITIDE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02383">
        <property type="entry name" value="Syja_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50275">
        <property type="entry name" value="SAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="77"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="SAC" evidence="6">
        <location>
            <begin position="146"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="688"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="698"/>
            <end position="714"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8026494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="844" mass="96495" checksum="2480C64659132DEA" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BIL1</accession>
    <name>A0A9Q1BIL1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Orange domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_32366</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BIL1"/>
    <dbReference type="OrthoDB" id="7693256at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.980">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003650">
        <property type="entry name" value="Orange_dom"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158457">
        <property type="entry name" value="Orange domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51054">
        <property type="entry name" value="ORANGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Orange" evidence="2">
        <location>
            <begin position="83"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8027265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="316" mass="34383" checksum="125050D70B9DF4BF" modified="2023-09-13" version="1">MKSDTSLDEENLLSMAVDYLKMAAATEKVTKRSRHEKEEKWAHRKKRKSRNTKKGSKVKEAPSHASHSNSSTEICWESALEGYVTGYKDCSQEAVRYLLEEEGLTPTDQIVIGLQNHLRDNEVGIDNNNRKSVESLVGQTLVCFNSSSAEATASPSSTSQQLPPIHELMSTSLSAFPTSSSCTNSLPQGSMTALTLATPGKQMEPLTVDTNQEYELPSPLQRGAEAAKKSKESLLTIAHTQPTISTPVYTHSLGHQVTPVTITAPMAPMAMSCATQRLGLEKAMATSLNTVGGQAYGFNLNYAAPYVYAWVKPAQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BIM0</accession>
    <name>A0A9Q1BIM0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Glutathione S-transferase omega-like 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_32380</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BIM0"/>
    <dbReference type="OrthoDB" id="2309723at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03190">
        <property type="entry name" value="GST_C_Omega_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000162">
        <property type="entry name" value="Glutathione S-transferase Gst3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047047">
        <property type="entry name" value="GST_Omega-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016639">
        <property type="entry name" value="GST_Omega/GSH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32419:SF6">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE OMEGA-LIKE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32419">
        <property type="entry name" value="GLUTATHIONYL-HYDROQUINONE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13410">
        <property type="entry name" value="GST_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13409">
        <property type="entry name" value="GST_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015753">
        <property type="entry name" value="GST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01206">
        <property type="entry name" value="Xi.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01148">
        <property type="entry name" value="Xi_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST C-terminal" evidence="4">
        <location>
            <begin position="166"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="53"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="189"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>glutathione</name>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="138"/>
            <end position="139"/>
        </location>
        <ligand>
            <name>glutathione</name>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Cys" evidence="3">
        <location>
            <position position="247"/>
        </location>
    </feature>
    <feature type="site" description="Lowers pKa of active site Cys" evidence="3">
        <location>
            <position position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR015753-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR015753-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR015753-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8027276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="322" mass="37182" checksum="CDEDB106413A02AA" modified="2023-09-13" version="1">MADDKKQSWTTSITKSGEFKRRESAFRNRITADGSSGFKAERDRYHLYVSLACPFAHRALIVRKLKGLEEIFPYTVVDWLLGENGWSFTDQKPKCTLDPVNGAKYLRELYFKADPNYDGNFTVPVVWDKKTNTIVNNESAEIIRMLNSEFNEFCGTPAQRELDLYPEALRSEIDELNSWIYPKINNGVYKSGFATSQEAYDKAVKDVFEGLDRVEEILSKKRYLTGNSITEADIRLLTSLLRFDTVYHGHFKCNKKRIIDYPNMWGFVRDLYAVDGISETVDQEHIQKCYQLSQRSINPHGILAIGPDIDFNAPHNRDKLFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BJZ5</accession>
    <name>A0A9Q1BJZ5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_30073</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8027966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BJZ5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8027966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="97" mass="11070" checksum="142DB80B7B0BB4EB" modified="2023-09-13" version="1">MKTAVTRRPVRNTGLNPSTSEWKVPETSNFNAAFLELSRSENCLRYVPILRLLSSIIASLVVIHSWNQFLRYSTLQVYYCTIVNVHALPALGPDQKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BLV0</accession>
    <name>A0A9Q1BLV0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_28359</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BLV0"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8029057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="78" mass="8952" checksum="C187452894E67EF7" modified="2023-09-13" version="1">MESSREFNFNAAFLELSRSENCLRYVAITTTVCSRRSSLVIIRSWNQFLRYSTLQVYHCAIVNVHALPALGPDQNTIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BNJ1</accession>
    <name>A0A9Q1BNJ1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">6-pyruvoyltetrahydropterin synthase</fullName>
            <ecNumber evidence="4">4.2.3.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_29622</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; tetrahydrobiopterin biosynthesis; tetrahydrobiopterin from 7,8-dihydroneopterin triphosphate: step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PTPS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.12" evidence="4"/>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8030048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BNJ1"/>
    <dbReference type="OrthoDB" id="14045at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003874">
        <property type="term" value="F:6-pyruvoyltetrahydropterin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006729">
        <property type="term" value="P:tetrahydrobiopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.479.10:FF:000003">
        <property type="entry name" value="6-pyruvoyl tetrahydrobiopterin synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.10">
        <property type="entry name" value="6-pyruvoyl tetrahydropterin synthase/QueD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007115">
        <property type="entry name" value="6-PTP_synth/QueD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038418">
        <property type="entry name" value="6-PTP_synth/QueD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12589:SF7">
        <property type="entry name" value="6-PYRUVOYL TETRAHYDROBIOPTERIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12589">
        <property type="entry name" value="PYRUVOYL TETRAHYDROBIOPTERIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01242">
        <property type="entry name" value="PTPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55620">
        <property type="entry name" value="Tetrahydrobiopterin biosynthesis enzymes-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0783">Tetrahydrobiopterin biosynthesis</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8030048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="146" mass="16743" checksum="C28F53F55B8F8194" modified="2023-09-13" version="1">MAATDSKLPIVTITRIQCFSAAHRLHSPHLSDEENKTIYGKCNHPNGHGHNYRAEVTLKGPVDPKTGMLCNIADLKVWIEEAIMKPMDHKHIDKDVEWFYTNPSTMENIAIFIWHQLKAVMTEPQLLHAVKLWETDNHFAIYKGES</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BPR9</accession>
    <name>A0A9Q1BPR9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Testis-specific serine/threonine-protein kinase 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_26860</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BPR9"/>
    <dbReference type="OrthoDB" id="6492282at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF68">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE ATG1C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000654">
        <property type="entry name" value="Integrin-linked_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="11"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8030429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="270" mass="30369" checksum="A05C625F9788F3A1" modified="2023-09-13" version="1">MMNEQLISHGYTPTKVLSCTCLGSRVILAQNPSGQAFALKSSAKKVSDEQYQETQRYLENEAKILSSLDHEHVVKLIETFHVNGSIVLVLEYMCGGTLGLFIRNSKKLHTDVAKKLANQLTDAITYLHTMNIAHCDIKSDNVLLDEQRNVKLADFGFAEDVRMASLKAIVWKGSPSFSSPEKLRGEVTNLLTGDVWSLGVVVFHMVTGYLPFGNETLHSILDTIDKPLRWPRPMKVIDSTCRDFVELILRTKESNRPTIQQLGQHSWLLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BRM3</accession>
    <name>A0A9Q1BRM3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Calmodulin, flagellar</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_28019</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8031324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BRM3"/>
    <dbReference type="OrthoDB" id="5983955at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0969">Cilium</keyword>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0282">Flagellum</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="463"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="500"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="400"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="121"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="408"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="425"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8031324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="561" mass="64705" checksum="C6318B83AFB1F870" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BUK5</accession>
    <name>A0A9Q1BUK5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Transient receptor potential cation channel subfamily A member 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HOLleu_23046</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Nanhai2018</strain>
            <tissue evidence="16">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8032948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="TRANSIENT RECEPTOR POTENTIAL CATION CHANNEL PROTEIN PAINLESS"/>
        <property type="match status" value="1"/>
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    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="3" id="KW-0716">Sensory transduction</keyword>
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        <location>
            <begin position="735"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="790"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="852"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="892"/>
            <end position="914"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="962"/>
            <end position="992"/>
        </location>
    </feature>
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            <begin position="88"/>
            <end position="120"/>
        </location>
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    <feature type="repeat" description="ANK" evidence="12">
        <location>
            <begin position="121"/>
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    <feature type="repeat" description="ANK" evidence="12">
        <location>
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    <feature type="repeat" description="ANK" evidence="12">
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    <feature type="repeat" description="ANK" evidence="12">
        <location>
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        </location>
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    <feature type="repeat" description="ANK" evidence="12">
        <location>
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        </location>
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    <feature type="repeat" description="ANK" evidence="12">
        <location>
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    <feature type="repeat" description="ANK" evidence="12">
        <location>
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    <feature type="repeat" description="ANK" evidence="12">
        <location>
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        <location>
            <begin position="786"/>
            <end position="998"/>
        </location>
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        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
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        <location>
            <begin position="1152"/>
            <end position="1200"/>
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        </location>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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            <dbReference type="SAM" id="Phobius"/>
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        <source>
            <dbReference type="Pfam" id="PF00520"/>
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    </evidence>
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            <dbReference type="EMBL" id="KAJ8032948.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1200" mass="134661" checksum="3E2367C015B6B901" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BUM6</accession>
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        <recommendedName>
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        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
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        </lineage>
    </organism>
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        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
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    <sequence length="165" mass="17998" checksum="AFAEC7FD478D4906" modified="2023-09-13" version="1">MTISICLKVCSERSLPVFGLEHGRYCYCGRHESVFETHGVAPDYECNIPCAGAQLCGGNNFIVFYKNDVFRGCYAFPSNDLNLFGGYVPTLTLDVCQIKCGNSYSGLTQGNKCYCSDELPREATYGSASDCARNSLYCRGSEKCGGSGHTAIWINEETQSKSGNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
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    <name>A0A9Q1BWP8_HOLLE</name>
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        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
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        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8034025.1"/>
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        <property type="entry name" value="Fibrinogen_C-term_domain"/>
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        <property type="entry name" value="Fibrinogen_C"/>
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        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8034025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="194" mass="22973" checksum="7A9DD4583F74549E" modified="2023-09-13" version="1">MTIGGGGWTVFQRRINGATNFYRNWYSYKNGFGNVRQEFWLGNEKLYYLTQQATYEYRADYVLSSNSPKFHTYTRFRIHNEANKYKVTDVGSNSGWGYSLYNTQNKAFSTYDQDNDGQSATNCAEGHQSGWWHGGYYYDYSYSYCYYYPEGSRHRYCSYANPNGVYNGGNGRNIYVYYYYCNTKYTELKIRRTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BWW6</accession>
    <name>A0A9Q1BWW6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Tubulin-specific chaperone cofactor E-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_21803</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BWW6"/>
    <dbReference type="OrthoDB" id="5855206at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18849:SF0">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 410-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18849">
        <property type="entry name" value="LEUCINE RICH REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="3">
        <location>
            <begin position="353"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8034803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="431" mass="49458" checksum="98067FB12CED6814" modified="2023-09-13" version="1">MQKRECVTMEEKTFVEAFTAKYLTEEEEDEEEDDDMTESGIVILCPKSKPSQASSYRRVILDRQSITSAGDCGQISEDLKNVRELDLSANHLRSWKEVEAIIRLMPRLEFLNLASTSLADSDRTDANGDDVELKQSVKVLVLNSTMISWTTVNVILSALPCVEEVHLSLNNFSNVPDPSHKILCTHHTSIQRLYFNDNKVTRWSEVAKIGQIFPNLKSLLLVNNFLTGLTDDIGLNFPHLEQLSIQDTKLSQWDEIDNLNTFTSLTEIQLQRIPLLEGLKAKESRQLVIARLPRITKLNNSPIFEAEREDAERHLIRKYMDEPDKPKRYEELFAMHKNVHRLAEVNLKPTESVVLTIKYEDQSTELEISVNQTCLQLKKYLASHFKVHSKSVRLCYLDMGINLGYDELKVPSASLHRFNMRDGDVILLEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BYP6</accession>
    <name>A0A9Q1BYP6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SCAN box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_19660</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8035853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BYP6"/>
    <dbReference type="OrthoDB" id="10063366at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF13">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCAN box" evidence="2">
        <location>
            <begin position="255"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="110"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="349"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="349"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="364"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="388"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8035853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="546" mass="62625" checksum="CBCBD7D320E0DEA9" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BZH4</accession>
    <name>A0A9Q1BZH4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_22481</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BZH4"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8035301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="133" mass="15131" checksum="B8BDC22776688EB5" modified="2023-09-13" version="1">MLQCKDFIRLRFYTDLLNFYVNSRIREVYIEFVKIGRSTMHSQTVYLLLVLSIAVAVVTAYEDDEFEDGMLFNLMDKRKGRRGKGKGGKGKGGFGFAWKDCPENEHCVCLMTSSGEVMAQGSCTYEHNKGYGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C0S1</accession>
    <name>A0A9Q1C0S1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="17">DNA topoisomerase 2-binding protein 1-A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HOLleu_20430</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Nanhai2018</strain>
            <tissue evidence="17">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Microtubule organizing center</location>
            <location evidence="3">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Cytoskeleton</location>
            <location evidence="4">Spindle pole</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the TOPBP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="251770at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033314">
        <property type="term" value="P:mitotic DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007095">
        <property type="term" value="P:mitotic G2 DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17731">
        <property type="entry name" value="BRCT_TopBP1_rpt2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17718">
        <property type="entry name" value="BRCT_TopBP1_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17749">
        <property type="entry name" value="BRCT_TopBP1_rpt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18434">
        <property type="entry name" value="BRCT_TopBP1_rpt5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17727">
        <property type="entry name" value="BRCT_TopBP1_rpt6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17738">
        <property type="entry name" value="BRCT_TopBP1_rpt7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17728">
        <property type="entry name" value="BRCT_TopBP1_rpt8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000018">
        <property type="entry name" value="DNA topoisomerase 2-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000028">
        <property type="entry name" value="DNA topoisomerase 2-binding protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000010">
        <property type="entry name" value="DNA topoisomerase II binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000020">
        <property type="entry name" value="DNA topoisomerase II binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000021">
        <property type="entry name" value="DNA topoisomerase II binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000029">
        <property type="entry name" value="DNA topoisomerase II binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059215">
        <property type="entry name" value="BRCT2_TopBP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057595">
        <property type="entry name" value="TopB1_SLF1_BRCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049542">
        <property type="entry name" value="TopBP1-like_BRCT0"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049936">
        <property type="entry name" value="TopBP1_BRCT_8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13561">
        <property type="entry name" value="DNA REPLICATION REGULATOR DPB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13561:SF20">
        <property type="entry name" value="DNA TOPOISOMERASE 2-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00533">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23294">
        <property type="entry name" value="BRCT_TopB1_SLF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12738">
        <property type="entry name" value="PTCB-BRCT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21298">
        <property type="entry name" value="TopBP1_BRCT0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="8"/>
    </dbReference>
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</entry>
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            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <source>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
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        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
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    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051242">
        <property type="entry name" value="WD-EF-hand_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44324:SF1">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44324">
        <property type="entry name" value="WD40 REPEAT DOMAIN 95"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="378"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="464"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="515"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="651"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1075"/>
            <end position="1117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="667"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1107"/>
            <end position="1117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8036619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1117" mass="127177" checksum="038D24E89DBD3494" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C2A2</accession>
    <name>A0A9Q1C2A2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_17528</name>
        <name evidence="3" type="ORF">HOLleu_17531</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8036875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8036877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C2A2"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040654158" evidence="1">
        <location>
            <begin position="20"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8036875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8036877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="111" mass="12443" checksum="0EAFBCDECE9EC947" modified="2023-09-13" version="1">MKFIFIFGLISVCLHVTIAKIVSLPPEMVAKNPVPEHGECFRNSRGYPAHRQGSLSNPALHSTAISASVMVTAWVPLVARRRLSRSSNRQCSVWSIWIPPLVLTSERRSKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C2K1</accession>
    <name>A0A9Q1C2K1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Acetylcholinesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_17742</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the type-B carboxylesterase/lipase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C2K1"/>
    <dbReference type="OrthoDB" id="408631at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003990">
        <property type="term" value="F:acetylcholinesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006581">
        <property type="term" value="P:acetylcholine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019695">
        <property type="term" value="P:choline metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050654">
        <property type="entry name" value="AChE-related_enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43918">
        <property type="entry name" value="ACETYLCHOLINESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43918:SF4">
        <property type="entry name" value="CARBOXYLIC ESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0719">Serine esterase</keyword>
    <feature type="domain" description="Carboxylesterase type B" evidence="4">
        <location>
            <begin position="13"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8037025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="263" mass="29880" checksum="51EBCCC02EE4AE0F" modified="2023-09-13" version="1">MLNPGGIIDTSIAIDTAYKIGRRVGCELAFTSNQLAECLRNVDEYELILAMDTVLLKLKPTVDGEFITAPPAKLVEEGLFKQASILVGTTLNDGVLGTVKTYPDQIFKRSPFSDRDDFRERLQKAYKFVNPLILDAMEQQYIDWSMADDPDANYFYTYMDIETDETFLCPQDAVSRKYAEGTNDVFRYSFTHEPSASVWPPVPVWKGVAHIDDVPFVFGSAFLPEFADVEVPQEEIDLSVDMMTYFTNFAKTGKSQLWRKRKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C2R7</accession>
    <name>A0A9Q1C2R7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_17928</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C2R7"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8037178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="65" mass="7294" checksum="9889DADE7456BC6A" modified="2023-09-13" version="1">MKRSGNNPELSNHSKSTGPGQEEICSMIRGTVCQQEAIYTNKLDSDGIKDPWLSSRTVESLYEEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C4N6</accession>
    <name>A0A9Q1C4N6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Centriolar coiled-coil protein of 110 kDa-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_18995</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8038029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C4N6"/>
    <dbReference type="OrthoDB" id="10028852at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007099">
        <property type="term" value="P:centriole replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032053">
        <property type="term" value="P:ciliary basal body organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903723">
        <property type="term" value="P:negative regulation of centriole elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032465">
        <property type="term" value="P:regulation of cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033207">
        <property type="entry name" value="CCP110"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13594">
        <property type="entry name" value="CENTRIOLAR COILED-COIL PROTEIN OF 110 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13594:SF1">
        <property type="entry name" value="CENTRIOLAR COILED-COIL PROTEIN OF 110 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16025">
        <property type="entry name" value="CaM_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="77"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="184"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="224"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="291"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="419"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="878"/>
            <end position="1008"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="542"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="104"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="193"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="245"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="339"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="428"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="460"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="888"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="929"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="966"/>
            <end position="981"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="992"/>
            <end position="1008"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8038029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1008" mass="111365" checksum="0E7DCB4C8D7F1E11" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C4P4</accession>
    <name>A0A9Q1C4P4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PRKCA-binding protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Protein interacting with C kinase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Protein kinase C-alpha-binding protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="ORF">HOLleu_19170</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="27">Nanhai2018</strain>
            <tissue evidence="27">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="20">Probable adapter protein that bind to and organize the subcellular localization of a variety of membrane proteins containing some PDZ recognition sequence. Involved in the clustering of various receptors, possibly by acting at the receptor internalization level. Plays a role in synaptic plasticity by regulating the trafficking and internalization of AMPA receptors. May be regulated upon PRKCA activation. May regulate ASIC1/ASIC3 channel. Regulates actin polymerization by inhibiting the actin-nucleating activity of the Arp2/3 complex; the function is competitive with nucleation promoting factors and is linked to neuronal morphology regulation and AMPA receptor (AMPAR) endocytosis. Via interaction with the Arp2/3 complex involved in regulation of synaptic plasicity of excitatory synapses and required for spine shrinkage during long-term depression (LTD). Involved in regulation of astrocyte morphology, antagonistic to Arp2/3 complex activator WASL/N-WASP function.</text>
    </comment>
    <comment type="subunit">
        <text evidence="23">Monomer and homodimer. Interacts with CXADR. Interacts presynaptically with the glutamate receptors GRIA2, GRIA3, GRIK3, isoform 3 of GRIA4, isoform A of GRM4, GRM7 and GRM8; with NAPA and NAPB; and with BTG2. The interaction with NAPA and NAPB disrupts the interaction with GRIA2, conducting to the internalization of GRIA2. Interacts with PRKCA; with the amine transporters SLC6A2 and SLC6A3; with the channels ASIC1 and ASIC2; with the GTP-binding proteins ARF1 and ARF3; with the ephrin receptor tyrosine kinases EPHA7, EPHB1 and EPHB2; with ERBB2 and through its PDZ domain with the C-terminal tail of PRLHR. Interacts with UNC5A. Interacts (via AH domain) with NCS1/FREQ; in a calcium-dependent manner. Interacts with F-actin and associates with the ARP2/3 complex. Interacts (via PDZ domain) with ARF1 (activated); the interaction blocks Arp2/3 complex inhibition. Interacts with SORCS3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Membrane</location>
            <topology evidence="4">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="22">Postsynaptic density</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="21">Synapse</location>
            <location evidence="21">Synaptosome</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="27">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8038168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C4P4"/>
    <dbReference type="OrthoDB" id="5917245at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098842">
        <property type="term" value="C:postsynaptic early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008021">
        <property type="term" value="C:synaptic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032588">
        <property type="term" value="C:trans-Golgi network membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019904">
        <property type="term" value="F:protein domain specific binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005080">
        <property type="term" value="F:protein kinase C binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097062">
        <property type="term" value="P:dendritic spine maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002092">
        <property type="term" value="P:positive regulation of receptor internalization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043113">
        <property type="term" value="P:receptor clustering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034315">
        <property type="term" value="P:regulation of Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07659">
        <property type="entry name" value="BAR_PICK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06722">
        <property type="entry name" value="PDZ_PICK1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000023">
        <property type="entry name" value="Interacting with PRKCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000073">
        <property type="entry name" value="Interacting with PRKCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010504">
        <property type="entry name" value="AH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030798">
        <property type="entry name" value="Arfaptin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037959">
        <property type="entry name" value="PICK1_BAR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12141">
        <property type="entry name" value="ARFAPTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12141:SF1">
        <property type="entry name" value="PRKCA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06456">
        <property type="entry name" value="Arfaptin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01015">
        <property type="entry name" value="Arfaptin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50870">
        <property type="entry name" value="AH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="15" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="16" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="17" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0564">Palmitate</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0770">Synapse</keyword>
    <keyword evidence="8" id="KW-0771">Synaptosome</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PDZ" evidence="25">
        <location>
            <begin position="19"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="AH" evidence="26">
        <location>
            <begin position="153"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="387"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="24">
        <location>
            <begin position="388"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="24">
        <location>
            <begin position="414"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00031097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00032804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00033721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00034102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00034105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00093501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="EMBL" id="KAJ8038168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="423" mass="47667" checksum="139AB0FD566C3999" modified="2023-09-13" version="1">MLDYELEEDTMGMQSVPGTVTLAKDSQNLIGISIGGGAPLCPCIYIVQIFDNTPAAKDGSLAAGDELVAVNGKTVKGRTKVEVARMIQAIKTEVTLSYNKLQADQKQGKSLDIVLKKVKHRMVEHMSSSTADALGLSRAILCNEDKGLLSNSFQTDVLIKKLEELQKTANLYKGMIEHSKRMLRSFYDLSQTHKAFGDLFSLIGAREPQPKACDAFQTFGESHRNMEKYGIHLLRTCKPMLADLGTFLTKAIPDTKLTIKKYADVKFEYLSFCLKVKEMDDEEYAYATLGEPLYRVETGNYEYRLILRCRQLARARFAQMRSDVMVKLELLDQKHVQDLVFHLNRLVSAMAQYLRNCHIQIKKAEVFPIEVDLLRTTMDYTHAHDFEDGFDEEEDGTEDQNSKTEEGESTGDGGMEHNLIDTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C4V6</accession>
    <name>A0A9Q1C4V6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Heparan sulfate glucosamine 3-O-sulfotransferase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_19121</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8038134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C4V6"/>
    <dbReference type="OrthoDB" id="411451at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008467">
        <property type="term" value="F:[heparan sulfate]-glucosamine 3-sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037359">
        <property type="entry name" value="NST/OST"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10605:SF72">
        <property type="entry name" value="HEPARAN SULFATE 3-O SULFOTRANSFERASE-B, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10605">
        <property type="entry name" value="HEPARAN SULFATE SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13469">
        <property type="entry name" value="Sulfotransfer_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040150817" evidence="4">
        <location>
            <begin position="27"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="active site" description="For sulfotransferase activity" evidence="2">
        <location>
            <position position="107"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR637359-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR637359-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8038134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="253" mass="28626" checksum="E918B4DC87484C96" modified="2023-09-13" version="1">MKSVRVVFSFLVLTTIIFCMWSWTHRQSESPTNHFPNNTLIGKLASAEIATASVGNAGIINNPDRYIHGCYSKGPIGTVQLLLDQNKLKTLGCTRRSPQVVIFGVRKGGTTTLRDFLSFHPQLSVTDTEIHYLEFLSRKSDARHPYATKFLYGERGNSVRVIGGEAYSNIFQPMERLKTYASLMPYSTPRQLVVEKTPKYFVCKNCPILLHRMIPDCKLIAILVDPVKRAISDYLDILRGYDHIEKIIFVVLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C550</accession>
    <name>A0A9Q1C550_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_15646</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C550"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8038269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="156" mass="15929" checksum="AE243165DEE2D308" modified="2023-09-13" version="1">MVLSCVVTRLVAKEVVSESPPVAMVVTVVVVSGSLFIPVVTSCVVSLSLVVADWVPSVRLVNGGVSSDTVSGVVVSTSVVRCVFNSVDCSTVVTSGSRVWESVVTCVVSPRVLNCVVSASAAVPPVVKLCEVVISVSPIVLPEDMVLSCVVTGLVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C609</accession>
    <name>A0A9Q1C609_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CRK SH3-binding GNRP</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_16669</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C609"/>
    <dbReference type="OrthoDB" id="25179at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00155">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.840.10:FF:000009">
        <property type="entry name" value="rap guanine nucleotide exchange factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.840.10">
        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008937">
        <property type="entry name" value="Ras-like_GEF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000651">
        <property type="entry name" value="Ras-like_Gua-exchang_fac_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019804">
        <property type="entry name" value="Ras_G-nucl-exch_fac_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023578">
        <property type="entry name" value="Ras_GEF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001895">
        <property type="entry name" value="RASGEF_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036964">
        <property type="entry name" value="RASGEF_cat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113:SF224">
        <property type="entry name" value="RAP GUANINE NUCLEOTIDE EXCHANGE FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00617">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00147">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00229">
        <property type="entry name" value="RasGEFN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48366">
        <property type="entry name" value="Ras GEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00720">
        <property type="entry name" value="RASGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50009">
        <property type="entry name" value="RASGEF_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50212">
        <property type="entry name" value="RASGEF_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-terminal Ras-GEF" evidence="6">
        <location>
            <begin position="823"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GEF" evidence="5">
        <location>
            <begin position="974"/>
            <end position="1201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="133"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="329"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="604"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="697"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="744"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="143"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="154"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="364"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="434"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="454"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="620"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="705"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="758"/>
            <end position="770"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00083313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8039070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1210" mass="137720" checksum="C6847925658341E3" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C801</accession>
    <name>A0A9Q1C801_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_13938</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8039819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C801"/>
    <dbReference type="OrthoDB" id="9048988at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33332">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="163"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8039819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="201" mass="22673" checksum="5871F9080E862F20" modified="2023-09-13" version="1">MLISEIIITLQPSLTLTVGKTSIHSVSSVRNLGVMFDSCFAMSSQVQALCCTLNHQLRNTARIRKYLDEDSCKHIVRALVTSCLDYGNSLLLGITKAQLHKLQHIQNRAACLICGDGSSEHVTPYLTRLHWLPVNQRIKFKLIVFVYLPYVSLSHHHHITSLLLYRIVLITLIIYAFGSFLTVLGFMFSKQLDPVVILHSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CA25</accession>
    <name>A0A9Q1CA25_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_15297</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CA25"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8040869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="125" mass="13971" checksum="48AE6293927D441C" modified="2023-09-13" version="1">MKENEFELSSDSTCYSKWKGCGVLVLPGCGVSSSTPPALFQLAENVLALARAYPENNCMMAHPMKLSHSYISTSCCGNQFKAPTMRTQGFISLKDSIATWYKERERHQLFWEGNLPPPPPHSFPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CBC4</accession>
    <name>A0A9Q1CBC4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Coactosin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_12565</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Binds to F-actin in a calcium-independent manner. Has no direct effect on actin depolymerization. Acts as a chaperone for ALOX5 (5LO), influencing both its stability and activity in leukotrienes synthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Interacts with 5-lipoxygenase (ALOX5/5LO) in a calcium-independent manner. Binds to F-actin with a stoichiometry of 1:2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the actin-binding proteins ADF family. Coactosin subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CBC4"/>
    <dbReference type="OrthoDB" id="20822at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005884">
        <property type="term" value="C:actin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030864">
        <property type="term" value="C:cortical actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030427">
        <property type="term" value="C:site of polarized growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030833">
        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11282">
        <property type="entry name" value="ADF_coactosin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000018">
        <property type="entry name" value="Coactosin-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002108">
        <property type="entry name" value="ADF-H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10829:SF29">
        <property type="entry name" value="COACTOSIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10829">
        <property type="entry name" value="CORTACTIN AND DREBRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00241">
        <property type="entry name" value="Cofilin_ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00102">
        <property type="entry name" value="ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51263">
        <property type="entry name" value="ADF_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ADF-H" evidence="9">
        <location>
            <begin position="2"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00058385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00062335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00068121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8041676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="144" mass="16225" checksum="DE32AB5E879BB7E1" modified="2023-09-13" version="1">MSGKIDTESAQEAYELVRNDTTPETWALLKYTDNKEIVLHRTGTDYDDMLSEFGEEERLYGYVRLEVGDELSKRAKFVLITYIGQKVSPLKKAAVSTDKAFVKMVFSQFQVEILADTLADVSKDKVEDRVRKAGGANYGTGVRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CBH8</accession>
    <name>A0A9Q1CBH8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ribosome biogenesis protein BOP1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_12583</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the PeBoW complex, which is required for maturation of 28S and 5.8S ribosomal RNAs and formation of the 60S ribosome.</text>
    </comment>
    <comment type="function">
        <text evidence="7">Required for maturation of ribosomal RNAs and formation of the large ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
            <location evidence="7">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
            <location evidence="7">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the WD repeat BOP1/ERB1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CBH8"/>
    <dbReference type="OrthoDB" id="5571054at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070545">
        <property type="term" value="C:PeBoW complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043021">
        <property type="term" value="F:ribonucleoprotein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000466">
        <property type="term" value="P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000061">
        <property type="entry name" value="Ribosome biogenesis protein BOP1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03027">
        <property type="entry name" value="BOP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028598">
        <property type="entry name" value="BOP1/Erb1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012953">
        <property type="entry name" value="BOP1_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17605:SF0">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN BOP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17605">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN BOP1 BLOCK OF PROLIFERATION 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08145">
        <property type="entry name" value="BOP1NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM01035">
        <property type="entry name" value="BOP1NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 7" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="2 7" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="3 8" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="BOP1 N-terminal" evidence="10">
        <location>
            <begin position="233"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="504"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="25"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="83"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="99"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="158"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="193"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="208"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00055102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8041693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="842" mass="97393" checksum="393B4883ABAD0638" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CCT8</accession>
    <name>A0A9Q1CCT8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_09363</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8042580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CCT8"/>
    <dbReference type="OrthoDB" id="6750869at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8042580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="172" mass="19419" checksum="E1E54F51D32AD906" modified="2023-09-13" version="1">MFRHIEKVIQSNADFTLNGNVILNIIHVDMANGKGRANTHTNLRQWLMKKKKSVITINNKDDLCLARALVTAKARLDKQSDPTINWNNIRKGQCEQTTLAKALHANAGVPEGPCGLDEVDRFQQFLQDYQILVISCLPKDPIMFKGPENDKKLCVLYQQGHFDVVTSLPGFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CDS2</accession>
    <name>A0A9Q1CDS2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Patched domain-containing protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_09734</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the patched family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8042863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CDS2"/>
    <dbReference type="OrthoDB" id="6510177at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1640.10">
        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051697">
        <property type="entry name" value="Patched_domain-protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003392">
        <property type="entry name" value="PTHD_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000731">
        <property type="entry name" value="SSD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10796">
        <property type="entry name" value="PATCHED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10796:SF92">
        <property type="entry name" value="PATCHED-RELATED, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02460">
        <property type="entry name" value="Patched"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82866">
        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50156">
        <property type="entry name" value="SSD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="21"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="263"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="295"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="329"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="368"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="400"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="483"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="696"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="721"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="749"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="793"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="823"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="domain" description="SSD" evidence="8">
        <location>
            <begin position="264"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="domain" description="SSD" evidence="8">
        <location>
            <begin position="674"/>
            <end position="845"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8042863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="881" mass="98359" checksum="B50D5A57BEB202E3" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CGB3</accession>
    <name>A0A9Q1CGB3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">AT-rich interactive domain-containing protein 4A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_07657</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10068428at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20389">
        <property type="entry name" value="Tudor_ARID4_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20390">
        <property type="entry name" value="Tudor_ARID4_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.60:FF:000003">
        <property type="entry name" value="AT-rich interactive domain-containing protein 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.60">
        <property type="entry name" value="ARID DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051232">
        <property type="entry name" value="ARID/SWI1_ChromRemod"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012603">
        <property type="entry name" value="ARID4A/B_PWWP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001606">
        <property type="entry name" value="ARID_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036431">
        <property type="entry name" value="ARID_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053820">
        <property type="entry name" value="MSL3_chromo-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002999">
        <property type="entry name" value="Tudor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13964:SF27">
        <property type="entry name" value="HAT-TRICK, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13964">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01388">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22732">
        <property type="entry name" value="MSL3_chromo-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08169">
        <property type="entry name" value="RBB1NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01014">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00501">
        <property type="entry name" value="BRIGHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00333">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46774">
        <property type="entry name" value="ARID-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51011">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0832">Ubl conjugation</keyword>
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        <location>
            <begin position="298"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="123"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="404"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="591"/>
            <end position="1315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="420"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="472"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="591"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="764"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="830"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
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            <end position="910"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
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            <end position="950"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
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            <end position="972"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="997"/>
            <end position="1035"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1044"/>
            <end position="1069"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1077"/>
            <end position="1086"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1101"/>
            <end position="1127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1130"/>
            <end position="1150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1186"/>
            <end position="1209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="1218"/>
            <end position="1235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="1245"/>
            <end position="1260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1291"/>
            <end position="1300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="1301"/>
            <end position="1311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8044801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1393" mass="159164" checksum="75EC39B877191413" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CGM3</accession>
    <name>A0A9Q1CGM3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">E3 ubiquitin-protein ligase</fullName>
            <ecNumber evidence="10">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_07110</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Ubiquitin ligase protein which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8 10">Belongs to the E3 ubiquitin-protein ligase UBR1-like family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="10"/>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="26387at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071596">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process via the N-end rule pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.30:FF:000001">
        <property type="entry name" value="E3 ubiquitin-protein ligase UBR2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1390.10:FF:000003">
        <property type="entry name" value="E3 ubiquitin-protein ligase UBR2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2670">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003769">
        <property type="entry name" value="ClpS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042065">
        <property type="entry name" value="E3_ELL-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014719">
        <property type="entry name" value="Ribosomal_bL12_C/ClpS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039164">
        <property type="entry name" value="UBR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055194">
        <property type="entry name" value="UBR1-like_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003126">
        <property type="entry name" value="Znf_UBR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21497:SF24">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE UBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21497">
        <property type="entry name" value="UBIQUITIN LIGASE E3 ALPHA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02617">
        <property type="entry name" value="ClpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22960">
        <property type="entry name" value="WHD_UBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02207">
        <property type="entry name" value="zf-UBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00396">
        <property type="entry name" value="ZnF_UBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54736">
        <property type="entry name" value="ClpS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51157">
        <property type="entry name" value="ZF_UBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBR-type" evidence="12">
        <location>
            <begin position="100"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="UBR-type" evidence="9">
        <location>
            <begin position="100"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1257"/>
            <end position="1313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU366018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8044378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1425" mass="163282" checksum="675A82497F4E520B" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CHW5</accession>
    <name>A0A9Q1CHW5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_07959</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CHW5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8045038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="70" mass="8251" checksum="3AD1EF22FE2C2F4A" modified="2023-09-13" version="1">MLAPVLETGIRKYLSNLSLGELSSHTLQEEEQERMSGTRTEHWKVSGQEGRKDLEWRQAESRERKRSYQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CIT0</accession>
    <name>A0A9Q1CIT0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">HOLleu_08507</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">Nanhai2018</strain>
            <tissue evidence="19">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CIT0"/>
    <dbReference type="OrthoDB" id="248923at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058923">
        <property type="entry name" value="RCC1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051997">
        <property type="entry name" value="STK_NEK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44535">
        <property type="entry name" value="PROTEIN CBG16200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44535:SF1">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE NEK9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25390">
        <property type="entry name" value="WD40_RLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00633">
        <property type="entry name" value="RCCNDNSATION"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00626">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12 19" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RCC1-like" evidence="18">
        <location>
            <begin position="83"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="15">
        <location>
            <begin position="114"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="15">
        <location>
            <begin position="171"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="15">
        <location>
            <begin position="225"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="15">
        <location>
            <begin position="277"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="15">
        <location>
            <begin position="344"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="15">
        <location>
            <begin position="397"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="481"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="568"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="638"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="492"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="520"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="569"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="585"/>
            <end position="604"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF25390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAJ8045488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="683" mass="73806" checksum="905A03A0A975FBA9" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CJ72</accession>
    <name>A0A9Q1CJ72_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Homeobox protein meis3-A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_09057</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TALE/MEIS homeobox family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CJ72"/>
    <dbReference type="OrthoDB" id="10056939at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000004">
        <property type="entry name" value="Meis2 homeobox isoform 2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008422">
        <property type="entry name" value="KN_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032453">
        <property type="entry name" value="PKNOX/Meis_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050224">
        <property type="entry name" value="TALE_homeobox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11850">
        <property type="entry name" value="HOMEOBOX PROTEIN TRANSCRIPTION FACTORS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05920">
        <property type="entry name" value="Homeobox_KN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16493">
        <property type="entry name" value="Meis_PKNOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 5 8" id="KW-0371">Homeobox</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="7">
        <location>
            <begin position="350"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="5">
        <location>
            <begin position="352"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
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            <begin position="195"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="268"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="448"/>
            <end position="467"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="6">
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            <end position="208"/>
        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
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            <end position="228"/>
        </location>
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            <end position="302"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="6">
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            <end position="323"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00009661"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8045936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="467" mass="51304" checksum="93FC6FDC7A0C2FDF" modified="2023-09-13" version="1">MAQRYAEDLPHYGSLSDCTSSMYDAHAPRSAIHYSSAYGQAAVPQLNHTPAVHPQFYPNHAPNVVTPVSSSVTDIHKRDKDAIYGHPLFPLLALIFEKCELATCTPREPGVAGGDVCSSESFNEDIAVFAKQARADKQYFSSNEELDSLMIQSIQVLRFHLLELEKVHELCDNFCHRYISCLKGKMPIDLVIEDREGGSSSKQGGVSSPTPPSLTENHSEARSPHDWGYKLGMTNKMRLSKLMDNNNENCDRQDRDPYFEVLHQSQNQTGLNSNSGLPANSNSGPGHGGYNQQPEDTHSNPGSDPPLTSMVSSVHSGDNSSETDGGLDGGLENSIGSGDGTEEDDDEKTARRQKKRGIFPKSATNIMRAWLFQHLTHPYPSEEQKKQLAQDTGLTILQVNNWFINARRRIVQPMIDQSNRAVPGMAGASPYGPDGQMGGYLMEAQQGPSHIPHLHPAAGNECDPHLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CKU6</accession>
    <name>A0A9Q1CKU6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cilia- and flagella-associated protein 54</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_05330</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2104158at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027912">
        <property type="entry name" value="CFAP54"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33487">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33487:SF1">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14858">
        <property type="entry name" value="CFAP54_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0966">Cell projection</keyword>
    <keyword evidence="2" id="KW-0969">Cilium</keyword>
    <keyword evidence="2" id="KW-0282">Flagellum</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="540"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="842"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1659"/>
            <end position="1700"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2010"/>
            <end position="2033"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2390"/>
            <end position="2425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2908"/>
            <end position="2931"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="3258"/>
            <end position="3289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="850"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2403"/>
            <end position="2416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="2908"/>
            <end position="2921"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="2922"/>
            <end position="2931"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="3268"/>
            <end position="3281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8046598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="3378" mass="375670" checksum="62298444B944982B" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CMT8</accession>
    <name>A0A9Q1CMT8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Helix-turn-helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_06415</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CMT8"/>
    <dbReference type="OrthoDB" id="10018421at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058912">
        <property type="entry name" value="HTH_animal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21301">
        <property type="entry name" value="REVERSE TRANSCRIPTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21301:SF10">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26215">
        <property type="entry name" value="HTH_animal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helix-turn-helix" evidence="1">
        <location>
            <begin position="99"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF26215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8047424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="155" mass="18063" checksum="1A102C01C9BD5C64" modified="2023-09-13" version="1">MASEKLQHFSNLTVKQVHTKTPLKPLLYFRYIDDIFFLWPHGLPSLNDFFSFCNNFHSSIKFTFDYSETEISFLDVLVKLGNSSIATTLFCKPTDTHGYLNFFSSHHISVKRAIVFSQCLRIRRICSSQSEYDKHVCNLVGYFLNNNYPLFIISP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CNJ9</accession>
    <name>A0A9Q1CNJ9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_01544</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CNJ9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33480">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33480:SF1">
        <property type="entry name" value="TYR RECOMBINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8049007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="182" mass="20974" checksum="5DE01F6B12C9BCE6" modified="2023-09-13" version="1">MRNFGNYLQNDSVLREGKGTLIVVYRPQYTIADPNDYLPCHERYGYYAKSEIWKHACPSHESRKNLDDDEPPKKKKKKKKRKRGTHCKKGKNLLPPIPGVSSKTSAILLNMSNDSVAQYCRSDSLIRMYADKLREKHGHDADQTSYIRQVRELGRLMLEYRSVTGQNNAQLSDILSPANLMM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CP56</accession>
    <name>A0A9Q1CP56_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF6570 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_00829</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CP56"/>
    <dbReference type="OrthoDB" id="8123539at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046700">
        <property type="entry name" value="DUF6570"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20209">
        <property type="entry name" value="DUF6570"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6570" evidence="2">
        <location>
            <begin position="7"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="195"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8048490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="434" mass="48674" checksum="51CA373D75A367E9" modified="2023-09-13" version="1">MMKLKIQCQAQVNNMKPSDQPPELSQLNCLERHLIAPVIPFMKISSLPKGAQKGLCGPVVCVVSNVTATAMQLPRLMDDDSLVKVKLKRKLQYKGHHLFQTVNPHRIHSALSILKTINPHYENISIEDENPVDFQEPLQSSTESTSEPVVNRITSTKNVITNCPVTSVTITEDTETNTGDLSLVENVQTCHSGNEIEQNSQNLDTVPSQDLSDIQNGNQQGESNLSQTSAPLYTCLQPTDIAQIVAEHVDDTVLCVAPGEGQQFLSIFNSEGPAFTTLFPDGKNTFKEQRMVKISPKRYFNARLLSYDTRFAQNPEYIFFAPYTTELHEILSSISIAMRKGSKRTSAGRIITSSMFQNKESIHQILSKDDGYYFMKKIRGTPAYWECSMRDLFAMVRQLGIPTWFCSFSAAGRRWTIIDEAILQQQGKAIPKKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CR49</accession>
    <name>A0A9Q1CR49_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_01978</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CR49"/>
    <dbReference type="OrthoDB" id="1095242at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032502">
        <property type="term" value="P:developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001485">
        <property type="entry name" value="Krueppel-related zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000097">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000060">
        <property type="entry name" value="zinc finger protein 436"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000145">
        <property type="entry name" value="Zinc finger protein 574"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000202">
        <property type="entry name" value="Zinc finger protein 574"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000624">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000710">
        <property type="entry name" value="Zinc finger protein 768"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001182">
        <property type="entry name" value="Zinc finger, C2H2 type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24381:SF393">
        <property type="entry name" value="CHROMATIN-LINKED ADAPTOR FOR MSL PROTEINS, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24381">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="20"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="282"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="310"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="338"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="366"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="400"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="428"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="456"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="689"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="718"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="746"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="774"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="808"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="836"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="864"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="892"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="920"/>
            <end position="947"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="948"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="976"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="1004"/>
            <end position="1031"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="1032"/>
            <end position="1059"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="9"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="197"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="29"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="212"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ8049295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1060" mass="121339" checksum="30712906E594A3EC" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CS73</accession>
    <name>A0A9Q1CS73_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_03156</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CS73"/>
    <dbReference type="OrthoDB" id="6421164at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8050091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="200" mass="22944" checksum="3A34C9C54AD6F8EB" modified="2023-09-13" version="1">MALFYVTLPSNSSPEVYPDNTLTHFRVKLPQPITLEGQWEVGLAEIVYPHQRYNLDEESTYFYTANGRQWWTKHIPMGYYRNEAGLLNVLETNLGSLIRYSWDDKSGKVTVHLKDVEVSFKGALAEILGFKGDTHITRSLTVENPMDIKHLHNLFVYCDIVEPHAVGHAKVPLIRVVTVKGKYGEDINSFFFFLAFFLST</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CSF7</accession>
    <name>A0A9Q1CSF7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_03798</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CSF7"/>
    <dbReference type="OrthoDB" id="5987933at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8050553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="123" mass="13694" checksum="9CA9B99B17C56BB1" modified="2023-09-13" version="1">MLQALEARNTCDLKFKSDAARVKGVSCLKGVEGLTVTPYDGSVAVTVLYLPFEVDQRLVARVLSQYGTVSEMRCCKYTAGELKGIFNGKRQFRMELVRDIPSFLFIGGSKAHIRLLLPAPYLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CSW1</accession>
    <name>A0A9Q1CSW1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Proline rich transmembrane protein 1B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_02770</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CD225/Dispanin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CSW1"/>
    <dbReference type="OrthoDB" id="6083617at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051423">
        <property type="entry name" value="CD225/Dispanin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007593">
        <property type="entry name" value="CD225/Dispanin_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14948">
        <property type="entry name" value="NG5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14948:SF44">
        <property type="entry name" value="PROLINE-RICH TRANSMEMBRANE PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04505">
        <property type="entry name" value="CD225"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="149"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="197"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="109"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="27"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="50"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8049849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="230" mass="24808" checksum="A0E86A6ADBBE4829" modified="2023-09-13" version="1">MSQPPYAPQHGYQQHPDYATHQAYPPQGYPPPQQGYPPPQQGYPPPQAYPPQQNHPAQYGYPQGQPPVASYQAQPPPAPLGTLPPIQGVATATTVHQPSSNVIVVTTSAAPPAPKRPQGQRRPAQATRQPPSYQKIVRNTQEGVQPPDYMAYAIIVTLFCCLPFGIIAIMRSSSVRSRFASGDYEGARRSSLSAKKWSNAGLICGTIGEIIIAIILLFSYVIVPAFAGLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CT28</accession>
    <name>A0A9Q1CT28_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DED domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_03627</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CT28"/>
    <dbReference type="OrthoDB" id="100767at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001875">
        <property type="entry name" value="DED_dom"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50168">
        <property type="entry name" value="DED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DED" evidence="1">
        <location>
            <begin position="19"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8050423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="205" mass="23205" checksum="B315C7456C56F329" modified="2023-09-13" version="1">MTSQMASSLWNPEKGPLIPYRGVLHDIAKHLKKKDIAHIAFQFPKLSTAQRDNIQDGIELFEELERIGELSATNIDRLIETIGKLQLQEPLIRLKEYKAEYLTSVSGEKTRNKSIASVRVISLENDTQAVEKHRNDAHGGKVESIDHIIENKQEKEQVDVLHEVSTEINSTVLMMQNTTLPCPQCDGFADSLQEEFQDEGIMVLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CTP9</accession>
    <name>A0A9Q1CTP9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Agenet-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_04314</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CTP9"/>
    <dbReference type="OrthoDB" id="6414146at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.70">
        <property type="entry name" value="Spindlin/Ssty"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008395">
        <property type="entry name" value="Agenet-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022894">
        <property type="entry name" value="Oligoribonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042567">
        <property type="entry name" value="SPIN/Ssty_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11046:SF29">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11046">
        <property type="entry name" value="OLIGORIBONUCLEASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05641">
        <property type="entry name" value="Agenet"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Agenet-like" evidence="3">
        <location>
            <begin position="317"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="149"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8050931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="451" mass="52344" checksum="9515D8DBFACF1C46" modified="2023-09-13" version="1">MDEVYMSLLAESDTDPLCKKILELMCASFAKLGERMLCDHLEGGKFWNVEDDVKHEMMSVPTTNVGVERDFGMLDRLMREKPNASTLALEGLIMWQENKTGKWRDELNEEMRAKYMRIARESMNEQRRLYFERRKAIKEVRAMRWAEKRERAVAKVERERERMVSLSNELKQVGGLWSSVSELEERLSALADEKEKCDALKVQLKYWKWVLKAKNKDGILNLSVAGKPKRFNDLLDSLKEVIQQQNHSFQGSLPDTSGVSGVTDGEDVTGSTLSLAKLGTKRRIGGDSLRVVPSSHNQKPLVDVKNLVGRVIKHCFEEEGVDVWYMGVVTKKLGKRLYIRYDSDKDNNGWYFDSETILEDYGRGDLYEITDLLNLNAVGRRVKHMWLDENTDEEDWWCGSVLSVKENEVVIEYDEVYVEDEDDSEEYEDERVTFPLEELQQAYLSRELLFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H1N0</accession>
    <name>A0A9Q1H1N0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Fibropellin-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">HOLleu_25089</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">Nanhai2018</strain>
            <tissue evidence="20">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
            <location evidence="2">Extracellular space</location>
            <location evidence="2">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6236007at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120025">
        <property type="term" value="C:plasma membrane bounded cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="CDD" id="cd00087">
        <property type="entry name" value="FReD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19941">
        <property type="entry name" value="TIL"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000162">
        <property type="entry name" value="Coagulation factor X (Predicted)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000247">
        <property type="entry name" value="Delta/notch like EGF repeat containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000066">
        <property type="entry name" value="FAT atypical cadherin 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000080">
        <property type="entry name" value="Neurogenic locus notch 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000143">
        <property type="entry name" value="Protein crumbs 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000122">
        <property type="entry name" value="Protein crumbs homolog 2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="26"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.300.10">
        <property type="entry name" value="Tie2 ligand-binding domain superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013032">
        <property type="entry name" value="EGF-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050780">
        <property type="entry name" value="Mucin_vWF_Thrombospondin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036084">
        <property type="entry name" value="Ser_inhib-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002919">
        <property type="entry name" value="TIL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025615">
        <property type="entry name" value="TILa_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014853">
        <property type="entry name" value="VWF/SSPO/ZAN-like_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001007">
        <property type="entry name" value="VWF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001846">
        <property type="entry name" value="VWF_type-D"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040941">
        <property type="entry name" value="GGGWT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11339">
        <property type="entry name" value="EXTRACELLULAR MATRIX GLYCOPROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11339:SF373">
        <property type="entry name" value="VWFD DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08742">
        <property type="entry name" value="C8"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12661">
        <property type="entry name" value="hEGF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01826">
        <property type="entry name" value="TIL"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12714">
        <property type="entry name" value="TILa"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00094">
        <property type="entry name" value="VWD"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00832">
        <property type="entry name" value="C8"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="28"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="SMART" id="SM00186">
        <property type="entry name" value="FBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00215">
        <property type="entry name" value="VWC_out"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00216">
        <property type="entry name" value="VWD"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57567">
        <property type="entry name" value="Serine protease inhibitors"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="22"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="21"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51233">
        <property type="entry name" value="VWFD"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="12 14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 14" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="4" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 16" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040294132" evidence="16">
        <location>
            <begin position="20"/>
            <end position="4084"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="213"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="349"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="387"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="425"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="463"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="501"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="539"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="577"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="615"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="653"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="691"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="729"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="767"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="805"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="843"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="881"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="919"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="957"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="995"/>
            <end position="1031"/>
        </location>
    </feature>
    <feature type="domain" description="VWFD" evidence="18">
        <location>
            <begin position="1036"/>
            <end position="1210"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="1388"/>
            <end position="1426"/>
        </location>
    </feature>
    <feature type="domain" description="VWFD" evidence="18">
        <location>
            <begin position="1431"/>
            <end position="1606"/>
        </location>
    </feature>
    <feature type="domain" description="VWFD" evidence="18">
        <location>
            <begin position="1823"/>
            <end position="2003"/>
        </location>
    </feature>
    <feature type="domain" description="VWFD" evidence="18">
        <location>
            <begin position="2221"/>
            <end position="2398"/>
        </location>
    </feature>
    <feature type="domain" description="VWFD" evidence="18">
        <location>
            <begin position="2691"/>
            <end position="2874"/>
        </location>
    </feature>
    <feature type="domain" description="VWFD" evidence="18">
        <location>
            <begin position="3094"/>
            <end position="3273"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="3448"/>
            <end position="3486"/>
        </location>
    </feature>
    <feature type="domain" description="VWFD" evidence="18">
        <location>
            <begin position="3490"/>
            <end position="3661"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="19">
        <location>
            <begin position="3872"/>
            <end position="4081"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="2537"/>
            <end position="2560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="2546"/>
            <end position="2555"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="237"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="375"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="413"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="451"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="489"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="527"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="565"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="603"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="641"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="679"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="717"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="755"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="793"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="831"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="869"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="907"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="945"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="983"/>
            <end position="992"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="1021"/>
            <end position="1030"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAJ8031782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="4084" mass="449460" checksum="2F506EB5065982FC" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H1S3</accession>
    <name>A0A9Q1H1S3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">peptidyl-tRNA hydrolase</fullName>
            <ecNumber evidence="1">3.1.1.29</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_29518</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>an N-acyl-L-alpha-aminoacyl-tRNA + H2O = an N-acyl-L-amino acid + a tRNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54448"/>
            <dbReference type="Rhea" id="RHEA-COMP:10123"/>
            <dbReference type="Rhea" id="RHEA-COMP:13883"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:59874"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:138191"/>
            <dbReference type="EC" id="3.1.1.29"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PTH2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.29" evidence="1"/>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H1S3"/>
    <dbReference type="OrthoDB" id="1733656at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004045">
        <property type="term" value="F:peptidyl-tRNA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1490.10:FF:000002">
        <property type="entry name" value="Peptidyl-tRNA hydrolase 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1490.10">
        <property type="entry name" value="Bit1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023476">
        <property type="entry name" value="Pep_tRNA_hydro_II_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002833">
        <property type="entry name" value="PTH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00283">
        <property type="entry name" value="arch_pth2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12649:SF29">
        <property type="entry name" value="AMINOACYL-TRNA HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12649">
        <property type="entry name" value="PEPTIDYL-TRNA HYDROLASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01981">
        <property type="entry name" value="PTH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22562">
        <property type="entry name" value="UBA_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102462">
        <property type="entry name" value="Peptidyl-tRNA hydrolase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBA" evidence="6">
        <location>
            <begin position="20"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8029968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="205" mass="21883" checksum="718C5371889FC5FE" modified="2023-09-13" version="1">MEPAGESAAEAGEAAEASPPSGETPELVKTLMTMGIEKELAMQAVWVTGSTTVDAALTWIFENREETAEVGEQERLPPDGALVEGENVAKMVFVVNMDLNMGVGKVAAQVAHAAVGLHKFLLQNQEVYGHLLLLWDADGETKVVLKGDNVTFLEDLRRKSEAASLPCYMVSDAGRTQIPAGSTTVLAVFGRVSEVDEITGKLKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H2Y6</accession>
    <name>A0A9Q1H2Y6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Tenascin-N</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_23488</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H2Y6"/>
    <dbReference type="OrthoDB" id="6145874at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.530.10">
        <property type="entry name" value="Gamma-fibrinogen Carboxyl Terminal Fragment, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050373">
        <property type="entry name" value="Fibrinogen_C-term_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020837">
        <property type="entry name" value="Fibrinogen_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143:SF462">
        <property type="entry name" value="APPLE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143">
        <property type="entry name" value="FIBRINOGEN/TENASCIN/ANGIOPOEITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00514">
        <property type="entry name" value="FIBRINOGEN_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="1">
        <location>
            <begin position="5"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8033297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="75" mass="8435" checksum="A517E2EDB9F4DFB9" modified="2023-09-13" version="1">MRTLQDIDRDNDLPLFTNCAVRHSGGWWFKSCYSSNLNGEYGRQDETGICLVDRSGAVPVNFCNITFSVMAMRPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H3D9</accession>
    <name>A0A9Q1H3D9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_27651</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8031050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H3D9"/>
    <dbReference type="OrthoDB" id="10545053at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040498309" evidence="1">
        <location>
            <begin position="24"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8031050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="262" mass="30269" checksum="5B9FE40386AFA434" modified="2023-09-13" version="1">MALPSSFLCIAVTVLGVCHLTVSQPLDSEEVSNFQAEVPDDLETAEILFQNEKNLAHQLALDYLYDGEEANDFTNFAKRDPRNEEMDKKDTRTLYDNYARRNIPPNFIKLNFGKRTTDKRFNSPFIFGKREDLSEVDKKAYSPFLYGKRERDLDLQPDKRAGYSKFMFGKRDGSEDDKRAGYSVLYFGKRVPEVAKRGFSKLYYGKRGSKGKGGYSKFVFGKREDMDKREDLAEDKRFKSSFYLGKRNFAADNDEVQAMENV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H3K5</accession>
    <name>A0A9Q1H3K5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_24484</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8034062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H3K5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCHC-type" evidence="2">
        <location>
            <begin position="97"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="97"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8034062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="129" mass="15025" checksum="23EC65179CFD8D04" modified="2023-09-13" version="1">MRPGTLEEALKVALDIETFYTAEKQRSYPGRRVVRAAEVEFKQSESVTKDPTGTATEEIRRCRQSMADLQSEIKAMRNELQLMKDERRQEEGTPIRFKCRKPGHMRRNCPEKRGNTTKQNEVSTRNDHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H3N5</accession>
    <name>A0A9Q1H3N5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">A disintegrin and metalloproteinase with thrombospondin motifs 20</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_25687</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H3N5"/>
    <dbReference type="OrthoDB" id="10035764at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002870">
        <property type="entry name" value="Peptidase_M12B_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11905">
        <property type="entry name" value="ADAM A DISINTEGRIN AND METALLOPROTEASE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11905:SF256">
        <property type="entry name" value="PEPTIDASE M12B DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01562">
        <property type="entry name" value="Pep_M12B_propep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M12B propeptide" evidence="2">
        <location>
            <begin position="81"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8032219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="362" mass="42261" checksum="E9C79EA36FA36587" modified="2023-09-13" version="1">MTSSTKTCDFNVSRGQRTRDSGQIFICFLSKISFNGMDRFSSNVVQRYHWADILTEDDLRYYARGDHEVEEFDIIYPLEDGKRSERSLSESILSTASLKLSAFNENLHLELFENPWLVPTDLKIKHMYETSVHEEIVHGKRCHFHGRSMNHNDSIAVISTCAGIHGLITFDDNEVFIEPLKNVHKSKLPRNVTYNIKQPHIIYKRRLKEGKIRSCGELKGQGYEKERKRKSIDGIDFEFYVGPKFMEIKLVLDVAFVTENSPNPTEYALALMNIASRRFVDPSLGFPIHLYLVSIVQFHYTEPFWDYSSLLYTFYSFQIWLNENKIEDDNDPNHWDHGILLSRCTTLFSPVTDIFTMRLIFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H4G9</accession>
    <name>A0A9Q1H4G9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_25607</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H4G9"/>
    <dbReference type="OrthoDB" id="6361178at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8032160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="88" mass="9736" checksum="287276C1A869ECB4" modified="2023-09-13" version="1">MPHIERKNPIVFGGGQRSPGVTGGQNLKTLLTQYLEMRYGKRKSPIIFGGGQRSSRVTGGQTLKTSLTRYLKKGSSDSSQTWYIDAPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H4T4</accession>
    <name>A0A9Q1H4T4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Replication factor A C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_25847</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H4T4"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013955">
        <property type="entry name" value="Rep_factor-A_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08646">
        <property type="entry name" value="Rep_fac-A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Replication factor A C-terminal" evidence="2">
        <location>
            <begin position="514"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="174"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="253"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="256"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="266"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8032341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="642" mass="72573" checksum="FE8BD3597AFDB5A9" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H6G2</accession>
    <name>A0A9Q1H6G2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA-directed DNA polymerase</fullName>
            <ecNumber evidence="2">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_21408</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="2"/>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H6G2"/>
    <dbReference type="OrthoDB" id="10064239at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.690">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1600.10">
        <property type="entry name" value="Palm domain of DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.960.10">
        <property type="entry name" value="VSR Endonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004868">
        <property type="entry name" value="DNA-dir_DNA_pol_B_mt/vir"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023211">
        <property type="entry name" value="DNA_pol_palm_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33568">
        <property type="entry name" value="DNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33568:SF3">
        <property type="entry name" value="DNA-DIRECTED DNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03175">
        <property type="entry name" value="DNA_pol_B_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0235">DNA replication</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA-directed DNA polymerase family B mitochondria/virus" evidence="9">
        <location>
            <begin position="406"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed DNA polymerase family B mitochondria/virus" evidence="9">
        <location>
            <begin position="741"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed DNA polymerase family B mitochondria/virus" evidence="9">
        <location>
            <begin position="944"/>
            <end position="1035"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8034528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1199" mass="139161" checksum="8D295080B9C8F230" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H6R2</accession>
    <name>A0A9Q1H6R2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Tyrosine-protein kinase receptor Tie-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_21814</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H6R2"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005112">
        <property type="term" value="F:Notch binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.300.10">
        <property type="entry name" value="Tie2 ligand-binding domain superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050906">
        <property type="entry name" value="Notch_signaling"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24044:SF506">
        <property type="entry name" value="NEUROGENIC LOCUS NOTCH HOMOLOG PROTEIN 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24044">
        <property type="entry name" value="NOTCH LIGAND FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69322">
        <property type="entry name" value="Tricorn protease domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0675">Receptor</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="515"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="1129"/>
            <end position="1163"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="539"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="1153"/>
            <end position="1162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8034813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1194" mass="130834" checksum="B55DD9623AE08C92" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H7Q0</accession>
    <name>A0A9Q1H7Q0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Monoacylglycerol lipase ABHD2-A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_19282</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily. AB hydrolase 4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8035565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H7Q0"/>
    <dbReference type="OrthoDB" id="5954035at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036126">
        <property type="term" value="C:sperm flagellum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097524">
        <property type="term" value="C:sperm plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008126">
        <property type="term" value="F:acetylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046464">
        <property type="term" value="P:acylglycerol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051792">
        <property type="term" value="P:medium-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051793">
        <property type="term" value="P:medium-chain fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048240">
        <property type="term" value="P:sperm capacitation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043401">
        <property type="term" value="P:steroid hormone receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050960">
        <property type="entry name" value="AB_hydrolase_4_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012020">
        <property type="entry name" value="ABHD4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10794">
        <property type="entry name" value="ABHYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10794:SF45">
        <property type="entry name" value="MONOACYLGLYCEROL LIPASE ABHD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005211">
        <property type="entry name" value="Ab_hydro_YheT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="domain" description="AB hydrolase-1" evidence="4">
        <location>
            <begin position="116"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="2">
        <location>
            <position position="196"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="2">
        <location>
            <position position="339"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="2">
        <location>
            <position position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR005211-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8035565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="427" mass="48714" checksum="8951A42D666CDCDB" modified="2023-09-13" version="1">MAHPLIIASTIIFLYYVVFRILKLTDQPVKPFLKYLDSKFNRNVLKVCEILNKPYVPPFLWGRCGHIQTILYAKFGRFQCPLPRGTRRSVIMPDGATQTFDIFEPHCRHVSGGDITLCVCPGIANSSEKKYVRTFVDYSQNQGYRVAVLNHIGALESVPITSPRIFTYGDTEEYDAMIQFVREHYPQSTLVSIGFSMGANIVVKYLGAEPHRQKYFLCALSVCQGYDCTRALDVLHEWESGRRIYNFMMTMNMLKHIRRNLNMLAGEGAKQFWQKNGLKPINYNVDKILSATSLCHIDEHLSRRMVGFPDIMGYYEDCSSINHLRKIQIPILLLHSEDDFLVPPKYNKCPLDYVDKAPNAIFAQTKHGGHLGFFEGGILKANTYSWLEKAVVGYVDAVYEVAVQQGIFPVIHDKLESSGNNCKIERS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H7Z3</accession>
    <name>A0A9Q1H7Z3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">[F-actin]-monooxygenase MICAL3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_16671</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H7Z3"/>
    <dbReference type="OrthoDB" id="20799at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050540">
        <property type="entry name" value="F-actin_Monoox_Mical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057494">
        <property type="entry name" value="Rossman_Mical"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23167:SF54">
        <property type="entry name" value="[F-ACTIN]-MONOOXYGENASE MICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23167">
        <property type="entry name" value="CALPONIN HOMOLOGY DOMAIN-CONTAINING PROTEIN DDB_G0272472-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25413">
        <property type="entry name" value="Rossman_Mical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding" evidence="4">
        <location>
            <begin position="105"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="[F-actin]-monooxygenase MICAL1-3-like Rossman" evidence="5">
        <location>
            <begin position="257"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="530"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="663"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="715"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="817"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="560"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="675"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="817"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="835"/>
            <end position="845"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF25413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8039072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="845" mass="94236" checksum="761A5848C790F0A2" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H869</accession>
    <name>A0A9Q1H869_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Zinc finger PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_20717</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H869"/>
    <dbReference type="OrthoDB" id="71166at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050863">
        <property type="entry name" value="CenT-Element_Derived"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004875">
        <property type="entry name" value="DDE_SF_endonuclease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007889">
        <property type="entry name" value="HTH_Psq"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19303:SF74">
        <property type="entry name" value="POGO TRANSPOSABLE ELEMENT WITH KRAB DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19303">
        <property type="entry name" value="TRANSPOSON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03184">
        <property type="entry name" value="DDE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05225">
        <property type="entry name" value="HTH_psq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Zinc finger PHD-type" evidence="5">
        <location>
            <begin position="637"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="419"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="493"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="588"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="423"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="493"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="505"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="539"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="588"/>
            <end position="620"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8036674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="690" mass="78834" checksum="EF5F3102112752DD" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H8V3</accession>
    <name>A0A9Q1H8V3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Globin C, coelomic</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_17482</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the globin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8036838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H8V3"/>
    <dbReference type="OrthoDB" id="436496at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019825">
        <property type="term" value="F:oxygen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005344">
        <property type="term" value="F:oxygen carrier activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01040">
        <property type="entry name" value="Mb-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.490.10">
        <property type="entry name" value="Globins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000971">
        <property type="entry name" value="Globin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009050">
        <property type="entry name" value="Globin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012292">
        <property type="entry name" value="Globin/Proto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013314">
        <property type="entry name" value="Globin_lamprey/hagfish"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044399">
        <property type="entry name" value="Mb-like_M"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46783">
        <property type="entry name" value="CYTOGLOBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46783:SF1">
        <property type="entry name" value="CYTOGLOBIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00042">
        <property type="entry name" value="Globin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00188">
        <property type="entry name" value="PLANTGLOBIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46458">
        <property type="entry name" value="Globin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01033">
        <property type="entry name" value="GLOBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0349">Heme</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0561">Oxygen transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Globin" evidence="6">
        <location>
            <begin position="20"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS01033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8036838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="169" mass="19450" checksum="B43881DBD51A671B" modified="2023-09-13" version="1">MGAAETKNEKNQTAVDETNGLSVEEKNLIRSSWEQIMKNKRKVGVDVFVRLFTNYPKSKELFEQLRDIPTEDLRNHKKMKAHALRVMASLNSVVESLDDLEILGEMLQNVGRTHVLHHVEKHYYDSLAVVLLEAFEDELKDKFNQKTRNAWTKAYGAMEKVIVEAYGDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HCZ8</accession>
    <name>A0A9Q1HCZ8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HYR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_12477</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HCZ8"/>
    <dbReference type="OrthoDB" id="412711at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003410">
        <property type="entry name" value="HYR_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02494">
        <property type="entry name" value="HYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50825">
        <property type="entry name" value="HYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040129610" description="HYR domain-containing protein" evidence="2">
        <location>
            <begin position="38"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="HYR" evidence="3">
        <location>
            <begin position="49"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8041609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="133" mass="13855" checksum="13DC25CB8E1F81F3" modified="2023-09-13" version="1">MQKTIVSHSSVSLCVECSFLLDHLLLCVLVQTHVGLSSEYTFDTLVTKVVNTPPSTPLCPTVDPVTSFGGNIGNFVSWSIGECTDAEDSSIIPVCDPPSGRLFSVGSTLVTCSCTDSSGLSSECTFNAVVTGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HEP9</accession>
    <name>A0A9Q1HEP9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Jerky protein-like-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_10226</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HEP9"/>
    <dbReference type="OrthoDB" id="10031330at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050863">
        <property type="entry name" value="CenT-Element_Derived"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004875">
        <property type="entry name" value="DDE_SF_endonuclease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007889">
        <property type="entry name" value="HTH_Psq"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19303:SF74">
        <property type="entry name" value="POGO TRANSPOSABLE ELEMENT WITH KRAB DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19303">
        <property type="entry name" value="TRANSPOSON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03184">
        <property type="entry name" value="DDE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05225">
        <property type="entry name" value="HTH_psq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH psq-type" evidence="3">
        <location>
            <begin position="18"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="DDE-1" evidence="2">
        <location>
            <begin position="212"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="537"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="541"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="574"/>
            <end position="586"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8043230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="596" mass="66332" checksum="4E7413C620D1E25E" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HER6</accession>
    <name>A0A9Q1HER6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Protein dispatched-like 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_10299</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the dispatched family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HER6"/>
    <dbReference type="OrthoDB" id="193905at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007224">
        <property type="term" value="P:smoothened signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1640.10">
        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052081">
        <property type="entry name" value="Dispatched_Hh_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053958">
        <property type="entry name" value="HMGCR/SNAP/NPC1-like_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000731">
        <property type="entry name" value="SSD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45951:SF3">
        <property type="entry name" value="PROTEIN DISPATCHED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45951">
        <property type="entry name" value="PROTEIN DISPATCHED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12349">
        <property type="entry name" value="Sterol-sensing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82866">
        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50156">
        <property type="entry name" value="SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="72"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="404"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="431"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="459"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="510"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="540"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="622"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="908"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="938"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="970"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1008"/>
            <end position="1032"/>
        </location>
    </feature>
    <feature type="domain" description="SSD" evidence="9">
        <location>
            <begin position="432"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8043275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1199" mass="134867" checksum="8DF5F1B01FCA6A41" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HES7</accession>
    <name>A0A9Q1HES7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">5-hydroxytryptamine receptor 1F</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_10332</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HES7"/>
    <dbReference type="OrthoDB" id="10011551at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00637">
        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45698:SF1">
        <property type="entry name" value="TRACE AMINE-ASSOCIATED RECEPTOR 13C-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45698">
        <property type="entry name" value="TRACE AMINE-ASSOCIATED RECEPTOR 19N-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="170"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="6">
        <location>
            <begin position="1"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8043305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="209" mass="23762" checksum="BE2CEA0037DB2622" modified="2023-09-13" version="1">MGTVVSLSMGLRALVDGGCNDLPEKCNQKFPQAAKAGKCTERTLQTSGRRMIAVVSITTTLVIPVVVMLYVYTSILRTLRPKNDLNVNQSIETDDMNVEVTTSSQAHEAKCHLHDPPRGRMKTARERRRQSVLTTMFLVCITYVICWTPNQILYFHYNFVSERTTFTDPIHRFSILLAASNAFLNPIIYAFKYRSFQKGLQHIVNGWRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HF28</accession>
    <name>A0A9Q1HF28_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Heparan sulfate glucosamine 3-O-sulfotransferase 3A1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_06316</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HF28"/>
    <dbReference type="OrthoDB" id="411451at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008467">
        <property type="term" value="F:[heparan sulfate]-glucosamine 3-sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037359">
        <property type="entry name" value="NST/OST"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10605:SF65">
        <property type="entry name" value="GH20068P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10605">
        <property type="entry name" value="HEPARAN SULFATE SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sulfotransferase" evidence="6">
        <location>
            <begin position="56"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="active site" description="For sulfotransferase activity" evidence="3">
        <location>
            <position position="64"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="287"/>
            <end position="291"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="271"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR637359-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR637359-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR637359-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8047337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="328" mass="37595" checksum="EF7F0A8432C3762A" modified="2023-09-13" version="1">MTALREKAIKENLIPPTYTGEIFQQGCYLYLPIGGPRIKIPSSLSDADKCEKRFPSVYIIGVKKSGTTTLGRYLDLHESLVLANNVRFVANNGSTVEQFVQQYLADMPFAMDDQTTVVNYPGYYWTNRKPFFKALPHLPPNPKIIAILRNPAKRALSDYLHMELTRIRNTKLHRHVPPFAGDDIKPTFENTVLLPNGSINASLAFVQQGLYAKHLSAFLEYIPKERILLLSGDEFAKNPLPSLKRVEQFLGLKSFYKDNYFVYNEEKGFFCAEIPTRPDWTCAGKFKGRSHPNVSEEVILKLAEFYKPHNRRLATQFNLSRHSFDWID</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HFS8</accession>
    <name>A0A9Q1HFS8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Serologically defined colon cancer antigen 8-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_07027</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HFS8"/>
    <dbReference type="OrthoDB" id="10252347at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007098">
        <property type="term" value="P:centrosome cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030010">
        <property type="term" value="P:establishment of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001764">
        <property type="term" value="P:neuron migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035148">
        <property type="term" value="P:tube formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031887">
        <property type="entry name" value="SDCCAG8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34343">
        <property type="entry name" value="SEROLOGICALLY DEFINED COLON CANCER ANTIGEN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34343:SF1">
        <property type="entry name" value="SEROLOGICALLY DEFINED COLON CANCER ANTIGEN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15964">
        <property type="entry name" value="CCCAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="360"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="18"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="35"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8044310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="531" mass="60997" checksum="DC64A141FEE30783" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HFY8</accession>
    <name>A0A9Q1HFY8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Ras-related protein Rab-1A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_07948</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HFY8"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01869">
        <property type="entry name" value="Rab1_Ypt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000069">
        <property type="entry name" value="Ras GTP-binding protein YPT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057289">
        <property type="entry name" value="Rab1/Ypt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0636">Prenylation</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="170"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8045029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="200" mass="22340" checksum="2C7826AE8993FC57" modified="2023-09-13" version="1">MLRDYLFKLLLIGDSGVGKSCLLLRFADDTYTESYISTIGVDFKIRTIELDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIVVYDVTDQESFNNVKQWLQEIDRYASENVNKLLVGNKCDLTTKKVVDYTTAKEYADQLDIPFLETSAKNATNVEQAFMTMAAEIKNRMGPSSVPSDGNRDKINPASTEVSGGKSGCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HGD6</accession>
    <name>A0A9Q1HGD6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_11367</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8044026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HGD6"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8044026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="174" mass="19368" checksum="F4244901B2C296A4" modified="2023-09-13" version="1">MAENMPTMIERNIRELRRLNAVPLDLSVRKRIKLDEETDSAVPPTPAEEIAQPSWPNPEGSSEASMMNVSPLEKTEETGTDTTEKNALVKAWLEINEGETPADAQPPCHGDACRAPISKRHIKRRLLPKGCKPKKLFKKQASSSSRGTNASMLQENVYYVSVPRKLFFLVNGAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HHT4</accession>
    <name>A0A9Q1HHT4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">UDP-D-xylose:L-fucose alpha-1,3-D-xylosyltransferase 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_03748</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 77 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HHT4"/>
    <dbReference type="OrthoDB" id="1712432at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005069">
        <property type="entry name" value="Nucl-diP-sugar_transferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052636">
        <property type="entry name" value="UDP-D-xylose:L-fucose_XylT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47032">
        <property type="entry name" value="UDP-D-XYLOSE:L-FUCOSE ALPHA-1,3-D-XYLOSYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47032:SF1">
        <property type="entry name" value="UDP-D-XYLOSE:L-FUCOSE ALPHA-1,3-D-XYLOSYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03407">
        <property type="entry name" value="Nucleotid_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleotide-diphospho-sugar transferase" evidence="2">
        <location>
            <begin position="70"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8050517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="275" mass="31941" checksum="55338EA2E15E6E87" modified="2023-09-13" version="1">MELTAIKNDVVNNETNQKTSHNNESYQFVNFTDVVRNLTSPVLLTTTNYGFLDLLLNLLFSIQRLSIQPMILILCEDKTVYVELLKRQSNFSLKYHLALTHLQESGNDAFGFSTTGYISLVKKRISYIEILLQRDLDVFYIDSDVVLLADPFKYFTDDNVDIFFQWDRNNACTGFFYIKANRTTKAFVTKWAEAMPGDQFGNQRAFNRAMRRSGEELKVYGLPKNLFMSGYEFRNSGKAWYLMSPQPVQIHANFIRGEGSKVDWLKKHELWLVNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HIL0</accession>
    <name>A0A9Q1HIL0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_04782</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HIL0"/>
    <dbReference type="OrthoDB" id="10051449at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8046183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="136" mass="15775" checksum="33C09E92B403D38D" modified="2023-09-13" version="1">MRRGKNKRRPRSCWVRPLAACRKETYDRLLSELRMDDQQSLFNLLGMPFEIFDELLSRVGPRISKRDINYRKALEPGLNSLQPYATLSGDRYPTLQYTFTVSRDTCALFITEVCQAIIDVLKDEVISCPGTPDAWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-06-18" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HIM8</accession>
    <name>A0A9Q1HIM8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Plexin-A4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HOLleu_04858</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Nanhai2018</strain>
            <tissue evidence="16">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the plexin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="125363at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002116">
        <property type="term" value="C:semaphorin receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017154">
        <property type="term" value="F:semaphorin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007162">
        <property type="term" value="P:negative regulation of cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050772">
        <property type="term" value="P:positive regulation of axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030334">
        <property type="term" value="P:regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00603">
        <property type="entry name" value="IPT_PCSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000131">
        <property type="entry name" value="Plexin A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.506.10">
        <property type="entry name" value="GTPase Activation - p120gap, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031148">
        <property type="entry name" value="Plexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013548">
        <property type="entry name" value="Plexin_cytoplasmic_RasGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046800">
        <property type="entry name" value="Plexin_RBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002165">
        <property type="entry name" value="Plexin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016201">
        <property type="entry name" value="PSI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001627">
        <property type="entry name" value="Semap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036352">
        <property type="entry name" value="Semap_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041019">
        <property type="entry name" value="TIG1_plexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041362">
        <property type="entry name" value="TIG2_plexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22625">
        <property type="entry name" value="PLEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22625:SF44">
        <property type="entry name" value="PLEXIN-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08337">
        <property type="entry name" value="Plexin_cytopl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20170">
        <property type="entry name" value="Plexin_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01437">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01833">
        <property type="entry name" value="TIG"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18020">
        <property type="entry name" value="TIG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17960">
        <property type="entry name" value="TIG_plexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00429">
        <property type="entry name" value="IPT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00423">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00630">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103575">
        <property type="entry name" value="Plexin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101912">
        <property type="entry name" value="Sema domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51004">
        <property type="entry name" value="SEMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 14" id="KW-0732">Signal</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040112131" evidence="14">
        <location>
            <begin position="22"/>
            <end position="1830"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1153"/>
            <end position="1179"/>
        </location>
    </feature>
    <feature type="domain" description="Sema" evidence="15">
        <location>
            <begin position="19"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ8046243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1830" mass="206650" checksum="867645DC99D14388" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HJJ0</accession>
    <name>A0A9Q1HJJ0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cortactin-binding protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">HOLleu_06389</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">Nanhai2018</strain>
            <tissue evidence="19">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Regulates the dendritic spine distribution of CTTN/cortactin in hippocampal neurons, and thus controls dendritic spinogenesis and dendritic spine maintenance. Associates with the striatin-interacting phosphatase and kinase (STRIPAK) core complex to regulate dendritic spine distribution of the STRIPAK complex in hippocampal neurons.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Interacts with CTTN/cortactin SH3 domain. Interacts with STRN, STRN4/zinedin and MOB4/phocein; this interactions mediate the association with the STRIPAK core complex and may regulate dendritic spine distribution of the STRIPAK complex in hippocampal neurons. Activation of glutamate receptors weakens the interaction with STRN and STRN4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Dendritic spine</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cell cortex</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6021133at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043197">
        <property type="term" value="C:dendritic spine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011704">
        <property type="entry name" value="ATPase_dyneun-rel_AAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050719">
        <property type="entry name" value="Cortactin-Actin_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019131">
        <property type="entry name" value="Cortactin-binding_p2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057568">
        <property type="entry name" value="CortBP2_NAV1-like_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23166:SF5">
        <property type="entry name" value="CTTNBP2 N-TERMINAL-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23166">
        <property type="entry name" value="FILAMIN/GPBP-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07728">
        <property type="entry name" value="AAA_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25408">
        <property type="entry name" value="AAA_lid_NAV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13637">
        <property type="entry name" value="Ank_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09727">
        <property type="entry name" value="CortBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="13" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="10" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0770">Synapse</keyword>
    <feature type="domain" description="Cortactin-binding protein-2 N-terminal" evidence="17">
        <location>
            <begin position="52"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="13">
        <location>
            <begin position="907"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="13">
        <location>
            <begin position="940"/>
            <end position="972"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="13">
        <location>
            <begin position="973"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="domain" description="ATPase dynein-related AAA" evidence="16">
        <location>
            <begin position="1279"/>
            <end position="1410"/>
        </location>
    </feature>
    <feature type="domain" description="CortBP2/NAV1-like AAA+ ATPase lid" evidence="18">
        <location>
            <begin position="1463"/>
            <end position="1534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="23"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="355"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="392"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="521"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="624"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1586"/>
            <end position="1677"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1715"/>
            <end position="1758"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="186"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="357"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="404"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="415"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="433"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="482"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="541"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="578"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="638"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="683"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="693"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="1661"/>
            <end position="1673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1715"/>
            <end position="1728"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00044767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF07728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF09727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF25408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAJ8047401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1770" mass="194835" checksum="1334D09C8E3C6B20" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HKF3</accession>
    <name>A0A9Q1HKF3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Enterin neuropeptide</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_00753</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HKF3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007218">
        <property type="term" value="P:neuropeptide signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34723:SF7">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34723">
        <property type="entry name" value="PROTEIN CBG17025"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0527">Neuropeptide</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="166"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="191"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="260"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8048436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="323" mass="35999" checksum="319A47C9A350003E" modified="2023-09-13" version="1">MIIYISFLPSFLPSILPSFLPSFLPSFLPSFLPSFLPSFLPSFLPSFLPSFLPSFLPSFLPFLPFPSFLPSFLPSFLPSFLPSFLPSFLPSFPSFLPSFLPSFLPSFLPSFLPSFLPSFLPSFLPSVLPCVRPSVPSSFLPSFLPSFLPSFLPSFLPSFLPSFLPSFLPSLLTSFLPSFLPSFLPSFLPSFLPSFLPSFLPSFLPSFLPSFLPSFPFPSSLPSSLPSFLPSFLPSFLPSFLPSFLPSFLPSFLPSFLPSFLSFFLSFFLSFFALVVHLQCLVGPVPLRSFLHVSREIRKWCPPPTSAARHIHKPVKQELKSNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HL19</accession>
    <name>A0A9Q1HL19_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF5641 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_01829</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HL19"/>
    <dbReference type="OrthoDB" id="8958038at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040676">
        <property type="entry name" value="DUF5641"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331:SF1">
        <property type="entry name" value="GAG-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18701">
        <property type="entry name" value="DUF5641"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF5641" evidence="1">
        <location>
            <begin position="90"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8049201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="190" mass="22034" checksum="B55AE82B80FA3280" modified="2023-09-13" version="1">MEFLQELRRFFAMRGQPAYILSDNGSQFVGAQKELQLMDIGNLVNQRPMGRVPIDPDDGHYLCPNDMLLSRSSREVPQGPFRETRNPKHRVEFVQKIVNSFWQRWTRDVLPLLVPRRKWDANRCNVCVNDIVMLVDSSAVRVKWTVGRVVEVYPGNDGKVRNVKVKTMSAEYRRPIAKIAVIYPVEGYED</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0Y8P9</accession>
    <name>A0A9Q0Y8P9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_44619</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010001051">
        <property type="protein sequence ID" value="KAJ8017752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0Y8P9"/>
    <dbReference type="OrthoDB" id="10072198at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46670">
        <property type="entry name" value="ENDO/EXONUCLEASE/PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46670:SF3">
        <property type="entry name" value="ENDONUCLEASE_EXONUCLEASE_PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="1">
        <location>
            <begin position="18"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8017752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="297" mass="33360" checksum="99C7F236FEC4D029" modified="2023-09-13" version="1">MNLLTIYLQNFRSIRNKAISVSDFVASNNIDILALTETWLNETETTVIANLVPVGYTFQHVPRPAGIGGGVGLLFKSGLTVKTRTSTKDKLYTHFEHMDCNVSTGSSQFTVGVIYRPPSSSKNRLKTSTFFEEWASYLETFTSKEDRLILMGDINLHLDNPHDLDSQRFNSLLDIHGLRQHVVGPTHRSGHTLDVVITQEDSEILYVPLSVTKACFGDDSDVTCDHDTISFTINVPKPQNVRKTVSYRKLSEINVPSFIADIKDCEDLKNLNRTVDELVAAYNLNLSKLLEPPNTRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YCA4</accession>
    <name>A0A9Q0YCA4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_43071</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000202">
        <property type="protein sequence ID" value="KAJ8018750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YCA4"/>
    <dbReference type="OrthoDB" id="6369833at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8018750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="142" mass="16434" checksum="E3B6BA9B0384D912" modified="2023-09-13" version="1">MDVTNIYLPYTDYRPKINGFVKSRWQELWDSSPENKLYQIRPTVGSGGHGAPSKRRDGIVLTRARIGHTYLTHAYLLHGDERPYCIPCDCAVTVSHILVDCYEYSHVRQKYYAVPDLKTLFEQTDPSLILGFLKESKLYKSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YDG3</accession>
    <name>A0A9Q0YDG3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">DnaJ-like subfamily C member 27</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_40303</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YDG3"/>
    <dbReference type="OrthoDB" id="8830751at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04119">
        <property type="entry name" value="RJL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000019">
        <property type="entry name" value="dnaJ homolog subfamily C member 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000697">
        <property type="entry name" value="DnaJ homolog subfamily C member 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="5">
        <location>
            <begin position="212"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8020653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="267" mass="30271" checksum="E8B8D6BA48399C8C" modified="2023-09-13" version="1">MDKNRLKKTIRVKVISMGNAETGKSCIIKRYCEKRFVSKYLSTIGIDYGVTKVHAKDREVKVNIFDMAGHPIFYEVRNEFYKDTQGALLVYDVTNRASFEALDSWIAEMKREIGDPSDMERVVFCVCANKVDKTSQRVVDDFEGHAWANSNGFHYFETSAASGNGINELFEKLFEGLVTAYENGGKVERINTTLGYTKEQIDAIQRLKNSTDDWKRLGLLPGASKDEINKAYRKYAVLLHPDKNIAPGSEEAFKILVSARTALLKNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YFJ6</accession>
    <name>A0A9Q0YFJ6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_41202</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8019559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YFJ6"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8019559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="54" mass="6215" checksum="D3EFFA9C13CF5958" modified="2023-09-13" version="1">MPEAPVLCGTKLTTNLDTILRWPTIAIHKYVWLLLPAFHAFVTQQYKLSSQDED</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YG79</accession>
    <name>A0A9Q0YG79_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">TNFR-Cys domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_39574</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8022162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YG79"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="CDD" id="cd00185">
        <property type="entry name" value="TNFRSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.10">
        <property type="entry name" value="Tumor Necrosis Factor Receptor, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001368">
        <property type="entry name" value="TNFR/NGFR_Cys_rich_reg"/>
    </dbReference>
    <dbReference type="SMART" id="SM00208">
        <property type="entry name" value="TNFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040280736" description="TNFR-Cys domain-containing protein" evidence="3">
        <location>
            <begin position="28"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="190"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="TNFR-Cys" evidence="4">
        <location>
            <begin position="76"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="132"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="133"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8022162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="249" mass="28471" checksum="77A1C1C9A904FA49" modified="2023-09-13" version="1">MPELQAYICLRLITAFLLLLCVYTISGDTGWQQNEVVYNITMNEDCFSNDTDACEPGYHLNHDNTPCHNDRKCCYCPPNTYQPWWNECVSCILYTNCSEMQKVTMHHCNITHNSVCGKHLVTTNVPEAEGTSIIPSTTQVSSHAITPEDEVVSNKLPEASDGTKEDEDEDDEQEGTVYRFWCVNYTLAHFFTHLLCLLPGVLFIAFPSWPRDINIAIKSSYNKWKRHYYRNSGRRPNRGSGLYNPPQGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YGM2</accession>
    <name>A0A9Q0YGM2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_41949</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8020099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YGM2"/>
    <dbReference type="OrthoDB" id="6278121at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045860">
        <property type="entry name" value="Snake_toxin-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57302">
        <property type="entry name" value="Snake toxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040251687" evidence="1">
        <location>
            <begin position="28"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8020099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="159" mass="18121" checksum="6055F24654D540BD" modified="2023-09-13" version="1">MVLRKVTTAACSLACICFLQLLTHVLSMECFKCPISLSLKECKEKQFVDQCIFGYTTCFTQIKYAAGSKDILNVTKSCVDDIFCERELKATDNHKCEIKDDNNYDCVWCCHRDRCNENSGVQSSKVNPSHTMVVTIVAFVCFYIQHLQRVSGLTMMETR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YI42</accession>
    <name>A0A9Q0YI42_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Sodium-coupled monocarboxylate transporter 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HOLleu_39246</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Nanhai2018</strain>
            <tissue evidence="15">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>iodide(out) + 2 Na(+)(out) = iodide(in) + 2 Na(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:71207"/>
            <dbReference type="ChEBI" id="CHEBI:16382"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YI42"/>
    <dbReference type="OrthoDB" id="6132759at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015075">
        <property type="term" value="F:monoatomic ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098660">
        <property type="term" value="P:inorganic ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="entry name" value="Sodium/glucose cotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018212">
        <property type="entry name" value="Na/solute_symporter_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051163">
        <property type="entry name" value="Sodium:Solute_Symporter_SSF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00813">
        <property type="entry name" value="sss"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42985:SF40">
        <property type="entry name" value="LD47995P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42985">
        <property type="entry name" value="SODIUM-COUPLED MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00456">
        <property type="entry name" value="NA_SOLUT_SYMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="11" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="79"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="123"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="150"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="184"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="236"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="279"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="331"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="377"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="407"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="434"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="505"/>
            <end position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00036099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ8021911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="568" mass="62102" checksum="155209654063E223" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YJ29</accession>
    <name>A0A9Q0YJ29_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Reverse transcriptase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HOLleu_38236</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Nanhai2018</strain>
            <tissue evidence="15">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8023139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="term" value="F:endonuclease activity"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000032">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 297-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon gypsy-like Protein"/>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
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        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
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        <property type="entry name" value="PROTEIN CBG26694"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Integrase_p58_C"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="RT_POL"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="3" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SCAN box" evidence="12">
        <location>
            <begin position="177"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="11">
        <location>
            <begin position="336"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase catalytic" evidence="14">
        <location>
            <begin position="734"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase" evidence="13">
        <location>
            <begin position="1147"/>
            <end position="1297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="271"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="377"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="27"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="271"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="288"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="298"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="317"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="377"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50804"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ8023139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1297" mass="146972" checksum="E36EE2CE8E5602A6" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YJG5</accession>
    <name>A0A9Q0YJG5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Somatostatin receptor type 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_38906</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YJG5"/>
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        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00637">
        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45695">
        <property type="entry name" value="LEUCOKININ RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45695:SF15">
        <property type="entry name" value="OPSIN RH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="88"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="142"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="9">
        <location>
            <begin position="1"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8021644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="251" mass="28529" checksum="E1608E4387E0A05F" modified="2023-09-13" version="1">MTMTAIAVDRYYAVRKPMVFKMKFSVKRTKILIISLWGLALIAAVPTAFMFKSVYVSQDDKSNYKGKTPFACKLSLPFGAWFKDFKAIYLNVVLFLLPVTITSILFVRIVLYVRQSNLAINKRFANAAGAIRNSHWTTAKSLLAVFVVFVFCYMPFSMYNIMQRYAPAVLSANFKNVALLLPYANSCLNPVVYSFTSERFRRYCVRLLCPCNRKTIATRHPSHSGDSAGTDQQSHSLQRFASILAIKDYKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YJS0</accession>
    <name>A0A9Q0YJS0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">General transcription factor IIH subunit 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_36399</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TFB1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YJS0"/>
    <dbReference type="OrthoDB" id="360521at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000439">
        <property type="term" value="C:transcription factor TFIIH core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13229">
        <property type="entry name" value="PH_TFIIH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000479">
        <property type="entry name" value="General transcription factor IIH subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005607">
        <property type="entry name" value="BSD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035925">
        <property type="entry name" value="BSD_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027079">
        <property type="entry name" value="Tfb1/GTF2H1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013876">
        <property type="entry name" value="TFIIH_BTF_p62_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12856">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03909">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08567">
        <property type="entry name" value="PH_TFIIH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00751">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140383">
        <property type="entry name" value="BSD domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50858">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BSD" evidence="8">
        <location>
            <begin position="102"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="BSD" evidence="8">
        <location>
            <begin position="183"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="353"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="353"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="372"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8023843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="582" mass="65242" checksum="C603C88F64BB4D23" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YKJ0</accession>
    <name>A0A9Q0YKJ0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Hexosyltransferase</fullName>
            <ecNumber evidence="11">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_38141</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Golgi apparatus membrane</location>
            <topology evidence="1 11">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the glycosyltransferase 31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="11"/>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8023066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YKJ0"/>
    <dbReference type="OrthoDB" id="2139606at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.50:FF:000001">
        <property type="entry name" value="Hexosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002659">
        <property type="entry name" value="Glyco_trans_31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214">
        <property type="entry name" value="BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214:SF314">
        <property type="entry name" value="HEXOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01762">
        <property type="entry name" value="Galactosyl_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040355639" description="Hexosyltransferase" evidence="12">
        <location>
            <begin position="24"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8023066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="331" mass="38173" checksum="CB5C3D825DA83E9B" modified="2023-09-13" version="1">MIVLLLFLPGPAIWLVVNHKSSAVPAKETSDELFDTRDTSSYNYHKSSFHVRGDVKLQRAPSTIPNLCKVTQKNTKEVFLLVLMVSAPMDFEQRKLQRLHCLNFSEISGQRVERLFLLGDSSNSSLNNRIKEESIIYQDIVMGNFHDSYRNLSLKTLFGLRWASMYCSQAHYVMKADDDTFINFKPLIDTLTRSPKENFITGHLCTGCRPIRNTTNKWYTPEEKFPGEFYPDYVHGPAYVMSGDLPRKISLMSRSTPPFPWEDVYIGMILEKLGIKPLQNPCFIADTHDWNLLTPCSYISACAIHFYNRREDIFQFCRDPIILNGNAREMC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YLN4</accession>
    <name>A0A9Q0YLN4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_36097</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YLN4"/>
    <dbReference type="OrthoDB" id="8963689at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8023610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="90" mass="10395" checksum="F8F82A90E8E0BB82" modified="2023-09-13" version="1">MPFNLDKFMASPSVEELDSLKKSEIVKVAKHNGIEFQPLMPKDEIKRYVPEYLVDESILPSTVLETAITVPTDNSFELKRLEMKLTRRLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YN62</accession>
    <name>A0A9Q0YN62_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ribonuclease P protein subunit p30</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_33127</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic/archaeal RNase P protein component 3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YN62"/>
    <dbReference type="OrthoDB" id="17948at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005655">
        <property type="term" value="C:nucleolar ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002738">
        <property type="entry name" value="RNase_P_p30"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13031:SF0">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13031">
        <property type="entry name" value="RIBONUCLEASE P SUBUNIT P30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01876">
        <property type="entry name" value="RNase_P_p30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="PHP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0819">tRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="250"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="250"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8025540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="283" mass="31917" checksum="A351A76808D74C00" modified="2023-09-13" version="1">MVKFYDLNIPRSNDAVAQKHTLKLAQRLGYEVVAISETLKYGDEMKPAAPPCDLSSEPSGEKLQQLSRITIVLTDSTRTDILRRSDVKSYDILAVQPTSEKTFHMACHDLNIDLITFDLSDRLEFHLKYSNVQNAWKAGKFFEICYAPAIRDATRRKHIFKAAAGLTDRKGGRNLIISSGALRPTELRGPYDVANLATLFGVDENSTKHCVSSYCRALLLKAATRKQTIKSSISVNYMSDIKDEDKWKVRQSLKESERDGGKREAEEKDEGEDRKGSKKLKAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YR71</accession>
    <name>A0A9Q0YR71_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_32019</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YR71"/>
    <dbReference type="OrthoDB" id="6782675at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8027009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="145" mass="16384" checksum="2B84DF974D831EA0" modified="2023-09-13" version="1">MPYFKGVSERITRILNRAGAKTFYSRSQKLRGDILSQPKDPLSKKHTACVYSIPCSCGQQYIGQTKRPLRVRVVELKRATEQGKAEGSALAEHAHCNNHTPLWADVTSIVKVKHLGMRLIREALEIRVNEKSTINRMDGKDVSHM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YS73</accession>
    <name>A0A9Q0YS73_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_33090</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YS73"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="66"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="266"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="533"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="844"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="742"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="13"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="67"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="107"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="118"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="266"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="313"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="535"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="567"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="619"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="844"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="854"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="934"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="989"/>
            <end position="1014"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8025512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1022" mass="115002" checksum="1AB321231F602194" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YSH7</accession>
    <name>A0A9Q0YSH7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Chondroadherin-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_31747</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YSH7"/>
    <dbReference type="OrthoDB" id="28057at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050541">
        <property type="entry name" value="LRR_TM_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369">
        <property type="entry name" value="ANTIGEN BSP, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369:SF210">
        <property type="entry name" value="CHAOPTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="472"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="domain" description="LRRCT" evidence="5">
        <location>
            <begin position="323"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="Immunoglobulin" evidence="6">
        <location>
            <begin position="379"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8026805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="520" mass="57233" checksum="15F50611A31E4FC7" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YSL4</accession>
    <name>A0A9Q0YSL4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HECT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_34867</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YSL4"/>
    <dbReference type="OrthoDB" id="10038899at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="HECT" evidence="4">
        <location>
            <begin position="246"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="7"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8024841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="421" mass="47771" checksum="6D2A8284C851D7D5" modified="2023-09-13" version="1">MGKVRRGNSSTPRNPLFQTTRQSKKRKVMLGWLHWKNNKVVQIRQKDGGGSRSALFSPSDGYDAIMDKCRSLFFPNGQSSFGKIEDMQTSLARFNRETIQPVKDGVEFSIKGYQEETKLSTLRIFLKTIPKSDWDNSSDDELELVDPFQSAQSLLVNTTPEERNTVEPVQVELIEPMDTESADHAEIIAPAENSPVAFGPLVGLDPVELDDTLPLDNMTDTNIPQVRSVEVHRTTVIKDCIHHFSDRNIMEDHLTLRYIDERGVDGGGVSRDVYSAFWIELFNSSAEGCNQVVPFLRNDMGWKEWEAVGRVLAKGFLDHNFFPIYLCKAFILGCLLGPDNISDTTLINSFIEYIPAEDRECLKKALSDMDNITDEENDDLLDILSRYRCRTLPRKETISKVVNTVAHKELLQKPKYIIDCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YTH6</accession>
    <name>A0A9Q0YTH6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SGNH hydrolase-type esterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_31895</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YTH6"/>
    <dbReference type="OrthoDB" id="5804959at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8026924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="260" mass="28822" checksum="24872EE84BB3CFDB" modified="2023-09-13" version="1">MWAEFVNLGLNCTFEEASRDTFVFVCQKCNRLKELEGLLAAADSSNGWVSVQKGRKPVEIVEGCPAVETSNSFSGLSVEDIVQEVVEESKSVVVCGRVKRLRVRYCLLGTPLLGILIGNFVRGTEGTGIEDTSARVGKIVGDEEVVALQVGTNNLSRDNQISLRSKYKELICRLKSTRWKGVVCGILPRFDRKVSWGKILGFNKWLKNECALEGFLFVDTWAGFVNRRDLFAEDGLHLWSIGANELGRLVSRAVDSQVSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1B8Y2</accession>
    <name>A0A9Q1B8Y2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_43728</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000431">
        <property type="protein sequence ID" value="KAJ8018336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1B8Y2"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8018336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="242" mass="27243" checksum="91224AB7D0C3FA96" modified="2023-09-13" version="1">MQDVVVASLDQTEDASSVASRGSRHSVVSTSSVTRMKEARIKSELARISVAQLQQAQQLDRERLECERKAQLFKVKCESERAEAEAKMWMAEVLAELEDTNRIRQAYIEPKVDLPSVTVPKPKFSTESWQPNPYAPEWNSDNHHPKSKTTIPDSVPPAGLAEPMETHNLGSGPDANASYIFEGMLHSLNMPHPGIITFDGNPSCYWNFMDSFETNVEARVKDDKMRLTYLIQFCKGKARESI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1B9L4</accession>
    <name>A0A9Q1B9L4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Zinc finger MYND domain-containing protein 10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_43756</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000444">
        <property type="protein sequence ID" value="KAJ8018320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1B9L4"/>
    <dbReference type="OrthoDB" id="6088515at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01753">
        <property type="entry name" value="zf-MYND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144232">
        <property type="entry name" value="HIT/MYND zinc finger-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01360">
        <property type="entry name" value="ZF_MYND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50865">
        <property type="entry name" value="ZF_MYND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MYND-type" evidence="5">
        <location>
            <begin position="57"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8018320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="112" mass="12930" checksum="F2A405C7D90E9091" modified="2023-09-13" version="1">MDVKPGLCWRCETRLQSKKELCFLCKIAVYCSTKCLERDEARHGSVECKMWSRINKCEACGRIGRMKECSGCYAAWFCDKTCQGFAWKSHKVECSKWTEKAREVALAKKICV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BA36</accession>
    <name>A0A9Q1BA36_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_44604</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010001036">
        <property type="protein sequence ID" value="KAJ8017763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BA36"/>
    <dbReference type="OrthoDB" id="10037236at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47510:SF3">
        <property type="entry name" value="ENDO_EXONUCLEASE_PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47510">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8017763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="222" mass="25344" checksum="D032EA1475CB93E4" modified="2023-09-13" version="1">MSGKIISNGCTADLEYITIKCQLYYLPREFSSVTLTSLYIHPKADTVVALNIIAYVISEYENRDPDTLSIIAGDFNPANLKTVLPSFKQHVTCPTRGQRTIDHCYCKVKSAYKAIERSGLGTSDYSVVLLILPRGAELKQRKPLDRNVTLWPQSDVEELRDCFECTDWSVFGTQCDLDEYKITVTDYLPFCQDVCLPTRKVTHYPNSKPWFNKSINSLKYCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BAF9</accession>
    <name>A0A9Q1BAF9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_40328</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BAF9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57603">
        <property type="entry name" value="FnI-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040146371" evidence="1">
        <location>
            <begin position="22"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8020676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="166" mass="19127" checksum="66726E899C183317" modified="2023-09-13" version="1">MMLVLWIITVAGCMSHHVVEGSQYLVTEEMEFPSYSESDTDFLFCDFRSILRFQTERWTEMCSQCTCERTAVLCNRNRCPAFCRHRFRLLNDYCCQGCTLKGNVTEALEIGLTCPPSEWSFVERSHDEEWRLDGCTSCVCNNGTSSCKSETCCSQCPSIKFHRNET</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BAT1</accession>
    <name>A0A9Q1BAT1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Galanin receptor type 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_40829</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8021066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BAT1"/>
    <dbReference type="OrthoDB" id="5781782at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00637">
        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45698:SF1">
        <property type="entry name" value="TRACE AMINE-ASSOCIATED RECEPTOR 13C-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45698">
        <property type="entry name" value="TRACE AMINE-ASSOCIATED RECEPTOR 19N-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="26"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="68"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="108"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="141"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="212"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="248"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="6">
        <location>
            <begin position="5"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8021066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="318" mass="35990" checksum="117F908A00B0CBEB" modified="2023-09-13" version="1">MGLIGNSVVIFIYTVKIRKYSSMNRLIRGLAVADLCTSAFLFPIPAIARTPNSLLGELYCRVIYPNNLMWISIVSSVYTLAMMSLEQCLAVVYPLRNRLMYSKSRSKIILSMVWLASFWTNAFTYFAAFRSHESGGCTYNLGGPLMQTCIGIFIFSLEYFIPMVIMVATHVKTLQCLHTQAEALMHHTGSRQDEKTRRESIMLRAHQRVLKMLLVVVLTFIFCWTADQIGFFLFNIGILEQSFLFSNVYQTFVVLAFVNSCANPFIYTASNPQFRKALKTFWNGQETSSLDSMNQGTMNVKFSTANTAATIMDLPTNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BB68</accession>
    <name>A0A9Q1BB68_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nose resistant to fluoxetine protein 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_43993</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000572">
        <property type="protein sequence ID" value="KAJ8018167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BB68"/>
    <dbReference type="OrthoDB" id="10054558at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052728">
        <property type="entry name" value="O2_lipid_transport_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11161">
        <property type="entry name" value="O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11161:SF0">
        <property type="entry name" value="O-ACYLTRANSFERASE LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8018167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="159" mass="18591" checksum="15C8B0B9958A13A8" modified="2023-09-13" version="1">MFILPHLLWGPRTPYYVGKQRCAEYWWTTMLFINNIYPNMTDLCMKWTWYLANDMQFFIVSPLFIYAFFRNPLVGLGLLSLLLTICMGATGFLVVYQDFTLPLYDAPWFPVTDLIYRAPFARVSPYLIGLLTGFILFKLKANKATFPRVSFTFSEDSNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BC85</accession>
    <name>A0A9Q1BC85_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Matrix metalloproteinase-24</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HOLleu_37287</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Nanhai2018</strain>
            <tissue evidence="17">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="11">Can bind about 5 Ca(2+) ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M10A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BC85"/>
    <dbReference type="OrthoDB" id="406838at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030574">
        <property type="term" value="P:collagen catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00094">
        <property type="entry name" value="HX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04278">
        <property type="entry name" value="ZnMc_MMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.110.10.10">
        <property type="entry name" value="Hemopexin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000585">
        <property type="entry name" value="Hemopexin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036375">
        <property type="entry name" value="Hemopexin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018487">
        <property type="entry name" value="Hemopexin-like_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033739">
        <property type="entry name" value="M10A_MMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001818">
        <property type="entry name" value="Pept_M10_metallopeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021190">
        <property type="entry name" value="Pept_M10A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036365">
        <property type="entry name" value="PGBD-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201">
        <property type="entry name" value="MATRIX METALLOPROTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201:SF323">
        <property type="entry name" value="MATRIX METALLOPROTEINASE-21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00045">
        <property type="entry name" value="Hemopexin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00413">
        <property type="entry name" value="Peptidase_M10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001191">
        <property type="entry name" value="Peptidase_M10A_matrix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00138">
        <property type="entry name" value="MATRIXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00120">
        <property type="entry name" value="HX"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50923">
        <property type="entry name" value="Hemopexin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47090">
        <property type="entry name" value="PGBD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51642">
        <property type="entry name" value="HEMOPEXIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="15" id="KW-0732">Signal</keyword>
    <keyword evidence="6 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040387586" evidence="15">
        <location>
            <begin position="23"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase metallopeptidase" evidence="16">
        <location>
            <begin position="112"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="13">
        <location>
            <begin position="304"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="13">
        <location>
            <begin position="350"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="13">
        <location>
            <begin position="398"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="271"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="278"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="225"/>
        </location>
    </feature>
    <feature type="binding site" description="in inhibited form" evidence="11">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="308"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="354"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="356"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>5</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="452"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>5</label>
        </ligand>
    </feature>
    <feature type="modified residue" description="Phosphotyrosine; by PKDCC" evidence="12">
        <location>
            <position position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR001191-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001191-2"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR621190-2"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR621190-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ8022401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="543" mass="60456" checksum="30E4A55DAC3903F6" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BCF7</accession>
    <name>A0A9Q1BCF7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_39968</name>
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    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BCF7"/>
    <dbReference type="OrthoDB" id="8961218at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
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        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8020388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="118" mass="13664" checksum="CFCF5BC489FC3FEE" modified="2023-09-13" version="1">MKDFDLKDIWRELSHNNYRYTWKSNSDPLIYCRLDYFLISFSLVNTVKSCEILAGLISDHNPITLSFESCFSGRGPGYWKLNVSLIKQCGLCTSNQRCCFSFLGYVCRLKSTFKMGLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BDR1</accession>
    <name>A0A9Q1BDR1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_40649</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BDR1"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8020927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="90" mass="10199" checksum="748DCBFBC7A0446C" modified="2023-09-13" version="1">MAKYPWIKDPKELPDNRQMAVVTLKSLEKRLGRDPGAGPGFLALPSNQWPVAAMRKPCQRVKVVMVEDVEEINALASKINVRKYSCYDKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BDU7</accession>
    <name>A0A9Q1BDU7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cadherin EGF LAG seven-pass G-type receptor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_40752</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8021002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BDU7"/>
    <dbReference type="OrthoDB" id="6134459at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15039">
        <property type="entry name" value="7tmB3_Methuselah-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053231">
        <property type="entry name" value="GPCR_LN-TM7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45902">
        <property type="entry name" value="LATROPHILIN RECEPTOR-LIKE PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45902:SF1">
        <property type="entry name" value="LATROPHILIN RECEPTOR-LIKE PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00249">
        <property type="entry name" value="GPCRSECRETIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="585"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="622"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="652"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="695"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="740"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="6">
        <location>
            <begin position="587"/>
            <end position="803"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8021002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="813" mass="92491" checksum="626CD4AA88B9AFA2" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BEN9</accession>
    <name>A0A9Q1BEN9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_35074</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BEN9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8024996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="78" mass="8901" checksum="7F2FC53010F0D3D6" modified="2023-09-13" version="1">MESSREFNFNAAFLELSRSENCLRNVPIIAATWQPSPSLVVIRSWNQFLRYSTLQVYYCTIVNVHALPALGPDQANIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BEY8</accession>
    <name>A0A9Q1BEY8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phospholipid scramblase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_39569</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">May mediate accelerated ATP-independent bidirectional transbilayer migration of phospholipids upon binding calcium ions that results in a loss of phospholipid asymmetry in the plasma membrane.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the phospholipid scramblase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8022157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BEY8"/>
    <dbReference type="OrthoDB" id="444338at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017128">
        <property type="term" value="F:phospholipid scramblase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005552">
        <property type="entry name" value="Scramblase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23248:SF9">
        <property type="entry name" value="PHOSPHOLIPID SCRAMBLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23248">
        <property type="entry name" value="PHOSPHOLIPID SCRAMBLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03803">
        <property type="entry name" value="Scramblase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0564">Palmitate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU363116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8022157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="277" mass="31980" checksum="44595DB5B1F20958" modified="2023-09-13" version="1">MLQVLAVTHPSMSDVNMSHPNHELIEMQPSNDPYADIDWMPKPLVGSTSPPGLEHLRQVDTLLVKETRDIYEALLHVPSIDTRNMFAMTNPAGERVYQGYEVSDYCTRIFCNELRKFQAHVVDEQGQELIRVIRPCQCCAGCCWFVCCRNFCSWRIRIECPPETTIGYVYQTWSIIRPQFVLQDEDHNDLYRLWGPNCSCQWLLGCTGSKEFRFTNSEGEEVARYAKARTGIPDGEKVRKPDSFRIKFHTDLDVKHKALIIGALFFLDVLYFEAEEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BFC0</accession>
    <name>A0A9Q1BFC0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">5-hydroxytryptamine receptor 1A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_33308</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BFC0"/>
    <dbReference type="OrthoDB" id="10010417at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071880">
        <property type="term" value="P:adenylate cyclase-activating adrenergic receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043410">
        <property type="term" value="P:positive regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14967">
        <property type="entry name" value="7tmA_amine_R-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248">
        <property type="entry name" value="ADRENERGIC RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248:SF66">
        <property type="entry name" value="OCTOPAMINE RECEPTOR BETA-3R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="25"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="145"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="188"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="343"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="380"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="45"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="297"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="321"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8025686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="419" mass="46623" checksum="543B242EE3E3B52A" modified="2023-09-13" version="1">MENITGTTFNQLLEEPKEFSLRTSLIYRVFLGLLISIMCAFGVIGNCLVAFAIARYPNLRTVTNYLIVSLAVADTTVCAWVMPIAAYQDLNGGRWRLGDILCDLWVGSDVLMSTASIWNLCAVSVDRYLAITRPIWYATKRKITFALILISVAWSLSFMNSVMALFIVGGFQGNEDAEFCAVNTQPSFGVASFFVAFFIPACAILVLYTLILRSAMRLTSSVRPLHMDPDQPSHYVGFSTRYPFTSKNEIKGTVEFANSAYGESGVPTTVTTTGDNKHTSGIVKTVSFEVQGSKTKRNEIPAPAASDQGPSQPSEPPGGTNEERRIKEKKERVKISMTKERRAALVIGIVVLAFIACWLPFFSVFFMISICTTCTVSLGIYQFVAWLGWCNSALNPVVYTVFNREFRQAFRKLFRRKDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BGD4</accession>
    <name>A0A9Q1BGD4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Adhesion G-protein coupled receptor G4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_34625</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BGD4"/>
    <dbReference type="OrthoDB" id="1100386at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15040">
        <property type="entry name" value="7tmB2_Adhesion"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.220.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053066">
        <property type="entry name" value="ADGR_G7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058808">
        <property type="entry name" value="GAIN_ADGRA2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057244">
        <property type="entry name" value="GAIN_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046338">
        <property type="entry name" value="GAIN_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000203">
        <property type="entry name" value="GPS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47767">
        <property type="entry name" value="ADHESION G PROTEIN-COUPLED RECEPTOR G7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47767:SF2">
        <property type="entry name" value="GPS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26588">
        <property type="entry name" value="GAIN_ADGRA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01825">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00249">
        <property type="entry name" value="GPCRSECRETIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00303">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50221">
        <property type="entry name" value="GAIN_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="942"/>
            <end position="964"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="976"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1000"/>
            <end position="1033"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1045"/>
            <end position="1065"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1085"/>
            <end position="1108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1134"/>
            <end position="1154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1160"/>
            <end position="1181"/>
        </location>
    </feature>
    <feature type="domain" description="GAIN-B" evidence="8">
        <location>
            <begin position="765"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="9">
        <location>
            <begin position="940"/>
            <end position="1183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="226"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="226"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="238"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8024663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1237" mass="138170" checksum="FD0671E6420B70F1" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BGI4</accession>
    <name>A0A9Q1BGI4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_34833</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BGI4"/>
    <dbReference type="OrthoDB" id="10678495at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030141">
        <property type="term" value="C:secretory granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051046">
        <property type="term" value="P:regulation of secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033522">
        <property type="entry name" value="IA-2/IA-2_beta"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46106">
        <property type="entry name" value="IA-2 PROTEIN TYROSINE PHOSPHATASE, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46106:SF4">
        <property type="entry name" value="IA-2 PROTEIN TYROSINE PHOSPHATASE, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040117337" evidence="2">
        <location>
            <begin position="21"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="407"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="429"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8024813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="469" mass="53582" checksum="8327F25E6E794D78" modified="2023-09-13" version="1">MAPGFLFLTRFLALLSLVSGGPYGNVGCHFSPVCLPDEICADDNLFGSCISLDSSHLPYYNLDPRGLREVKNSIKFLIDENLEWKDEISQVLEMEELVRVKYHISDRNLFVKESMLISGSLSNNEGILSKILYPYQIEYLRSENPFYNEDYNEKYPARTQEEQADEGPLRLRWNGLGSGAVRSKKYAYDGRYPSNEYHSEPSVPDLVEEYLTLLNELQASRENIFQQRFQEYYNRYLKLLERYNEYREYGIPLNPPSGSAGYQKPVDDLGDKMELIKDYLQKVYGQELTDDGKFVDGAWVDEDKIWNDNDAEVNPPTVGENGDEAENLEENLAEWLDESSSSSVPTPDEIMFPESSADDTEQDLLFLLGEQLIPYLSKADREEWDKLTEDEKMKMLEQVLDTVAGFDDEDENVNPENIPKMVFPEINDGGDKRVEDAEEREVDKDDPGKIKVSKGEGGGKGGEDWGRAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BGL4</accession>
    <name>A0A9Q1BGL4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Multiple myeloma tumor-associated protein 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_34995</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BGL4"/>
    <dbReference type="OrthoDB" id="5390672at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019315">
        <property type="entry name" value="MMTA2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039207">
        <property type="entry name" value="MMTAG2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14580:SF0">
        <property type="entry name" value="MULTIPLE MYELOMA TUMOR-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14580">
        <property type="entry name" value="MULTIPLE MYELOMA TUMOR-ASSOCIATED PROTEIN 2 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10159">
        <property type="entry name" value="MMtag"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Multiple myeloma tumor-associated protein 2-like N-terminal" evidence="2">
        <location>
            <begin position="8"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="91"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="173"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="211"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8024933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="223" mass="26164" checksum="0BE1EB765566AE61" modified="2023-09-13" version="1">MYYVPRGGNRGGADQFNWEDVKEDKGRECYLGHSLMAPVGRWQKNKDLQWFSKERKNTSTASISSEVQAARNAEREAMLIALGHKVGDRLAAETMQPQKDGAELSTLRSKLVKAKKKQKEKKSKKEKKVHKKRRHKEKRKKEKSDDHDETDSSSDSTDSSESERERPVKKRKESKEDHFRTRTDKHSSDHDRPNRGKHSQSSSQRDWHRSKSDRRKHKHTHRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BH01</accession>
    <name>A0A9Q1BH01_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1</fullName>
            <ecNumber evidence="6">2.4.1.122</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">HOLleu_34815</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="23">Nanhai2018</strain>
            <tissue evidence="23">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="19">Glycosyltransferase that generates the core 1 O-glycan Gal-beta1-3GalNAc-alpha1-Ser/Thr (T antigen), which is a precursor for many extended O-glycans in glycoproteins.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer; disulfide-linked.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyltransferase 31 family. Beta3-Gal-T subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.122" evidence="6"/>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BH01"/>
    <dbReference type="OrthoDB" id="414175at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016263">
        <property type="term" value="F:glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.50:FF:000017">
        <property type="entry name" value="Glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026050">
        <property type="entry name" value="C1GALT1/C1GALT1_chp1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003378">
        <property type="entry name" value="Fringe-like_glycosylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23033">
        <property type="entry name" value="BETA1,3-GALACTOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23033:SF14">
        <property type="entry name" value="GLYCOPROTEIN-N-ACETYLGALACTOSAMINE 3-BETA-GALACTOSYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02434">
        <property type="entry name" value="Fringe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="17" id="KW-0464">Manganese</keyword>
    <keyword evidence="14 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="15"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="Fringe-like glycosyltransferase" evidence="22">
        <location>
            <begin position="165"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="374"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="383"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00040898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00059245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF02434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KAJ8024799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="449" mass="51299" checksum="DB8CCA69B34EE359" modified="2023-09-13" version="1">MAGSILASGTSTQHLFINFIFGLLFGSVVTYIGLKFSNSSVVDELYVRVLERENPFLTLIADPHGDTHAEVNDGNKVSLQESQRFSAHKDAFMLGEDALGDWMSRRIRILCWIMTNPGNLDKKAIHVKATWAKRCNIVLFISSQNTDFPTIAVVDHEGREYLWQKTRGAFDHIYKHYLDKADWFLKADDDTYVIVENMRKLVSLYDPEKPIYFGRKFKPYVKQGYMSGGAGYVLSRKAVELLVEKGFKDTKLCKSATHVGGAEDVEIGRCLMNVGVEAGDSRDDIYETFHPFVMQQMVVPNILPRKFWYFSYNFYPVKEGPECCSDYSISFHYVPPNMMYQLEFMVYHMRPFGVGYYMCPAEIRTIIGLPEAQPPAEPASPIEGEKIKFVEKKEPEKKPEEKPESGKEQESEKKKESKKSKESEKKEEAKEETKVVVDIKAQDKEAVNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BHG0</accession>
    <name>A0A9Q1BHG0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">Phosphoacetylglucosamine mutase</fullName>
            <shortName evidence="14">PAGM</shortName>
            <ecNumber evidence="4 14">5.4.2.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12 14">Acetylglucosamine phosphomutase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11 14">N-acetylglucosamine-phosphate mutase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">HOLleu_32945</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="23">Nanhai2018</strain>
            <tissue evidence="23">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the conversion of GlcNAc-6-P into GlcNAc-1-P during the synthesis of uridine diphosphate/UDP-GlcNAc, a sugar nucleotide critical to multiple glycosylation pathways including protein N- and O-glycosylation.</text>
    </comment>
    <comment type="function">
        <text evidence="13">Catalyzes the conversion of GlcNAc-6-P into GlcNAc-1-P during the synthesis of uridine diphosphate/UDP-GlcNAc, which is a biosynthetic precursor of chitin and also supplies the amino sugars for N-linked oligosaccharides of glycoproteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>N-acetyl-alpha-D-glucosamine 1-phosphate = N-acetyl-D-glucosamine 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:23804"/>
            <dbReference type="ChEBI" id="CHEBI:57513"/>
            <dbReference type="ChEBI" id="CHEBI:57776"/>
            <dbReference type="EC" id="5.4.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14 17">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="14 17">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route I): step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.3" evidence="4 14"/>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BHG0"/>
    <dbReference type="OrthoDB" id="1928at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004610">
        <property type="term" value="F:phosphoacetylglucosamine mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006048">
        <property type="term" value="P:UDP-N-acetylglucosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03086">
        <property type="entry name" value="PGM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.50:FF:000003">
        <property type="entry name" value="Phosphoacetylglucosamine mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.120.10:FF:000013">
        <property type="entry name" value="Phosphoacetylglucosamine mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.120.10:FF:000023">
        <property type="entry name" value="Phosphoacetylglucosamine mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.120.10">
        <property type="entry name" value="Alpha-D-Glucose-1,6-Bisphosphate, subunit A, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.50">
        <property type="entry name" value="Alpha-D-phosphohexomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005844">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016055">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I/II/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005843">
        <property type="entry name" value="A-D-PHexomutase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036900">
        <property type="entry name" value="A-D-PHexomutase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016066">
        <property type="entry name" value="A-D-PHexomutase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049023">
        <property type="entry name" value="AMG1_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049022">
        <property type="entry name" value="AMG1_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016657">
        <property type="entry name" value="PAGM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45955">
        <property type="entry name" value="PHOSPHOACETYLGLUCOSAMINE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45955:SF1">
        <property type="entry name" value="PHOSPHOACETYLGLUCOSAMINE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21405">
        <property type="entry name" value="AMG1_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21404">
        <property type="entry name" value="AMG1_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02878">
        <property type="entry name" value="PGM_PMM_I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00408">
        <property type="entry name" value="PGM_PMM_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016408">
        <property type="entry name" value="PAGM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55957">
        <property type="entry name" value="Phosphoglucomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53738">
        <property type="entry name" value="Phosphoglucomutase, first 3 domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00710">
        <property type="entry name" value="PGM_PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="10" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="8 14" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7 14 17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 14 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="20">
        <location>
            <begin position="56"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="20">
        <location>
            <begin position="118"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphoacetylglucosamine mutase AMG1" evidence="22">
        <location>
            <begin position="180"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphoacetylglucosamine mutase AMG1" evidence="21">
        <location>
            <begin position="301"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase C-terminal" evidence="19">
        <location>
            <begin position="547"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="414"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="415"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="441"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphoserine intermediate" evidence="15">
        <location>
            <position position="66"/>
        </location>
    </feature>
    <feature type="binding site" description="via phosphate group" evidence="17">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="280"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="282"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="284"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="374"/>
            <end position="376"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="572"/>
            <end position="576"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="581"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00059527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR016408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR016408-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR016408-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR016408-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF02878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF21404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF21405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KAJ8025399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="621" mass="67801" checksum="059F85E07A102A7B" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BQH5</accession>
    <name>A0A9Q1BQH5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Cytochrome P450 2D9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HOLleu_27595</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Nanhai2018</strain>
            <tissue evidence="15">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8031007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BQH5"/>
    <dbReference type="OrthoDB" id="1470350at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016712">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006805">
        <property type="term" value="P:xenobiotic metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000238">
        <property type="entry name" value="Cytochrome P450 2A6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050182">
        <property type="entry name" value="Cytochrome_P450_fam2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300">
        <property type="entry name" value="CYTOCHROME P450 508A4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300:SF375">
        <property type="entry name" value="CYTOCHROME P450 FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 13" id="KW-0349">Heme</keyword>
    <keyword evidence="10 13" id="KW-0408">Iron</keyword>
    <keyword evidence="12 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0492">Microsome</keyword>
    <keyword evidence="11" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="13"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="13">
        <location>
            <position position="449"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ8031007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="536" mass="61259" checksum="6A43F40141FDDEC3" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BQM5</accession>
    <name>A0A9Q1BQM5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Transcription factor Dp-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_27361</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the E2F/DP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BQM5"/>
    <dbReference type="OrthoDB" id="552115at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14458">
        <property type="entry name" value="DP_DD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000047">
        <property type="entry name" value="Transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.80:FF:000001">
        <property type="entry name" value="Transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.80">
        <property type="entry name" value="Transcription factor DP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037241">
        <property type="entry name" value="E2F-DP_heterodim"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003316">
        <property type="entry name" value="E2F_WHTH_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038168">
        <property type="entry name" value="TF_DP_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014889">
        <property type="entry name" value="Transc_factor_DP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015648">
        <property type="entry name" value="Transcrpt_fac_DP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12548">
        <property type="entry name" value="TRANSCRIPTION FACTOR DP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12548:SF9">
        <property type="entry name" value="TRANSCRIPTION FACTOR DP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08781">
        <property type="entry name" value="DP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02319">
        <property type="entry name" value="WHD_E2F_TDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01138">
        <property type="entry name" value="DP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01372">
        <property type="entry name" value="E2F_TDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144074">
        <property type="entry name" value="E2F-DP heterodimerization region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="E2F/DP family winged-helix DNA-binding" evidence="10">
        <location>
            <begin position="177"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription factor DP C-terminal" evidence="9">
        <location>
            <begin position="269"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="134"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="425"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="463"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="134"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="468"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8030830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="511" mass="56066" checksum="4FB39CC2BB865349" modified="2023-09-13" version="1">MSEAATTLTNLWVGDSSITATTPTKVSVGNDDIMSFYQESGLDNLAREAGVLTTTSKPIVVKEQASPQQVTKVIPAMLHVVPSPKGKQQILPRIQLATSGTTVVPQMQIITTPQRQGSATTILQAISGTPQHILSSPSTVITPQSSSSEWGSATKRRGEPTELDFGNGTGKRKRREKEGKGLRHFSMKVCEKVQAKQVTSYNEVADELVREFTAPFHPTPMSPSEQSQYDQKNIRRRVYDALNVLMAMNIISKEKKEIRWIGLPTNSRQECDKLEREKKLRLERIKQKRSQLHELILQQIAFKNLVQRNKALEAEGSGPPTSNSAIHLPYIIVNTSKKTVIDCSISNDKFEYLFNFDNTFEIHDDIEVLKRMGMAYSLEKGQCDPLDVEKAKRMVPKALEAYVDDMATKSKEAFSNSIVAGPSGIHTHGASSSKSAYQPGDPQLSPLAMPLSQTDEEVAMATAGADFTASQDSSLGSFSDAGQSRGATDTPSSIMESESDEETIAFLDHGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BQW1</accession>
    <name>A0A9Q1BQW1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_27605</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8031014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BQW1"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8031014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="103" mass="11951" checksum="ADED41C0312E68DC" modified="2023-09-13" version="1">MPEKLISFSAVPPKEYAYVSCVFDGIFQLLRREWSTAYIHSNFFSLSPLPCIHERTHELFPSFSLFISLCLLIFLFPPFLFPFPSYVLSLSSISLYFPLFLSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BR03</accession>
    <name>A0A9Q1BR03_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TTF-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_27883</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8031222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BR03"/>
    <dbReference type="OrthoDB" id="10059235at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006580">
        <property type="entry name" value="Znf_TTF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45749">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45749:SF28">
        <property type="entry name" value="ZINC FINGER MYM-TYPE PROTEIN 1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00597">
        <property type="entry name" value="ZnF_TTF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TTF-type" evidence="2">
        <location>
            <begin position="71"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8031222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="219" mass="24809" checksum="32E07C1819C95F95" modified="2023-09-13" version="1">MSSSSQSFTSSSSGDISRSSSSKSFPSEAEKERSTNEPTAHEISAVEQPYQPYQPCPSDSVRIPPKVTKSQNVNFQYKWFQMWPWLHYSESLKGVLCFTCSSAYQQGLLDLANKKEDAFISTGFNNWKKVTGLGKHKLGRFQEYEMSETHRFAAVQLKAQKQATVVAQVAEHKCKQQELNRRMLVKVFTSVRYLAHQGLPLRHAELCVLSMQHPRAGTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BSD8</accession>
    <name>A0A9Q1BSD8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_25000</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BSD8"/>
    <dbReference type="OrthoDB" id="775972at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8031716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="111" mass="12786" checksum="FCFE36DB0613C9A4" modified="2023-09-13" version="1">MVGLGILEKWTAVSRRHLSCLLVNLVEVTESVVITINSVLKTREHPLQTSQELLNQSGRQKFTKLDLSNAYQQVILDKESRQFVCINTPLGLYRYTRHSFAISSGKKLWRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BSV3</accession>
    <name>A0A9Q1BSV3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_25725</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BSV3"/>
    <dbReference type="OrthoDB" id="5970526at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8032252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="225" mass="25726" checksum="465F4CF49B1ED792" modified="2023-09-13" version="1">MGRVSKAILDRINNTIRSRIRVNQWRNNSSVLKWFTNLDKKKNLTFLVFDIVDFYPSISEELLSKCLLWAKRYASISNTEHSAILNARRSLLYDHKGNTWEKRETNNQFDVSMGAYDGAEVCELVGLYILYQLEIQLGVDSVGLYRDDGLAVMRSFSGSNADRIRKDLVKVFQSCGLKITVQTNLRSVNFPDVTLNLNTASYQPYRNQMTSRSTSTASPTIHPQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BT06</accession>
    <name>A0A9Q1BT06_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_25826</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BT06"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8032327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="64" mass="7538" checksum="AF3F9AAE3CBDF89A" modified="2023-09-13" version="1">MASNFLRSRYFVNSCYVPLIIAQERIYLRLNTYGIYQRSLLPPSLHLQYCTCCIVVNTFNLQQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BVN7</accession>
    <name>A0A9Q1BVN7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Fibrinogen C domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_23763</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BVN7"/>
    <dbReference type="OrthoDB" id="7250310at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00087">
        <property type="entry name" value="FReD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19941">
        <property type="entry name" value="TIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050373">
        <property type="entry name" value="Fibrinogen_C-term_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020837">
        <property type="entry name" value="Fibrinogen_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040941">
        <property type="entry name" value="GGGWT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143">
        <property type="entry name" value="FIBRINOGEN/TENASCIN/ANGIOPOEITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143:SF444">
        <property type="entry name" value="PROTEIN SCABROUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00186">
        <property type="entry name" value="FBG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00514">
        <property type="entry name" value="FIBRINOGEN_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040481919" evidence="2">
        <location>
            <begin position="24"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="3">
        <location>
            <begin position="43"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="3">
        <location>
            <begin position="290"/>
            <end position="504"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8033503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="504" mass="57731" checksum="F4EC7B8AAF1DAE09" modified="2023-09-13" version="1">MHRFLFQVTQLTILCLTAERLLAEHMGNHQMQNSVEQGSLFVYQRPEYPRDCEEVRSQCSFDKSRGVHLIKPDGYPEPFEIYCDNVNSDAWTVLLRRNDGKLVFQRDWEEYKDGFGFLSGEFWIGNEKLSYLTNQKKYELHINITYANGSLCFINYDQFATTDDHGNYKITKLGNYSGNAGDYIPVCPRDMLPGDCSCQKTCANLSGCLNTCNADNTCVCYDGFYLKGEDCVPLTECTTCEVDGVNILEGGFYVNPGCTIRADCRNGQLTWDNTFTCSANAVCDDRNNVRQCYTDDVDCLDVYNKGFTTSNVYTIKPTDWPGSAFQVYCNMTDGGGWTVFQRRVDGTVDFYRDWDSYKVGFGSPGHEHWLGNDKLHYLSTQKRYTIRIDLVNLDNSPYYAKYDYFQINDESDNYRLSQLGSHSGNADSYSLAYHRNYPFSTYDRDNDGSGCPVSYHGAWWYCTCHDSNLNGRYDTNSCSLDDIVWDRLPGSDCNTFAEMKLRPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BVP0</accession>
    <name>A0A9Q1BVP0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ryncolin-4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_23932</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BVP0"/>
    <dbReference type="OrthoDB" id="6275059at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050373">
        <property type="entry name" value="Fibrinogen_C-term_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143">
        <property type="entry name" value="FIBRINOGEN/TENASCIN/ANGIOPOEITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00186">
        <property type="entry name" value="FBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="106"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="2">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8033625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="163" mass="18856" checksum="ABB88EB20A538042" modified="2023-09-13" version="1">MYVRSALFATVNVRNFHTHFLILQVIQRRVNDSVDFQRTYEDYQKGFGNLKGNFWLGNEKIFLLTNQMSYQLRIDRTFHESYFTVYSNFRISNSTQRFQLEELGEIIAGLCMCVCVGVCMCVCFFSCVCVCVFFSCSFVVTLSNCKLESLLDIARTGIEIVST</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BWZ4</accession>
    <name>A0A9Q1BWZ4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Helix-turn-helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_20901</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BWZ4"/>
    <dbReference type="OrthoDB" id="10063818at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058912">
        <property type="entry name" value="HTH_animal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21301">
        <property type="entry name" value="REVERSE TRANSCRIPTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21301:SF10">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26215">
        <property type="entry name" value="HTH_animal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helix-turn-helix" evidence="2">
        <location>
            <begin position="11"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8034175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="195" mass="22303" checksum="2BE4928C52B35E2F" modified="2023-09-13" version="1">MPYMKQNNTSLYVHRESNHPPSIIKNIPKAVNRRLSTISSNDAVFDAAAPPYQEALTKSGYQHQLRYEPPSRNNNNEKRKRKRNITWFNPPYSANVATNVGKKFLNLLEKCFPPGHQLRKILNRNTVKISYSCMPNVHQIITSHNKSILRAEKTADRARNTRSCNCRKDNPCPLEGNCLASGIIYQALVTRQDTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BYE8</accession>
    <name>A0A9Q1BYE8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_21821</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BYE8"/>
    <dbReference type="OrthoDB" id="8961218at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31635">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31635:SF196">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8034819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="185" mass="21141" checksum="42A5F1ADCB3C2A15" modified="2023-09-13" version="1">MLLERQALLSNSSPDDIDNLLRSEVEGAFIRSRVKYLEDGEKPSSFFFKQESRKASQKIIHSVRNNSGDIVTDDNDIACVFHNFYSNLFSREDNVDHNMQNEFIKFLSVTVDPSDKMNLDGPISLEEVKAALMNMSNNKSPGLDGIPYELYRKFFDIMGNDLTDVYNGIFLRALFLSHNALLLFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BYM1</accession>
    <name>A0A9Q1BYM1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Sushi domain-containing protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_22549</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BYM1"/>
    <dbReference type="OrthoDB" id="6051552at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005533">
        <property type="entry name" value="AMOP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051495">
        <property type="entry name" value="Epithelial_Barrier/Signaling"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003886">
        <property type="entry name" value="NIDO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001846">
        <property type="entry name" value="VWF_type-D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13802">
        <property type="entry name" value="MUCIN 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13802:SF65">
        <property type="entry name" value="NIDOGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03782">
        <property type="entry name" value="AMOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06119">
        <property type="entry name" value="NIDO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00094">
        <property type="entry name" value="VWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00723">
        <property type="entry name" value="AMOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50856">
        <property type="entry name" value="AMOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51220">
        <property type="entry name" value="NIDO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51233">
        <property type="entry name" value="VWFD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0768">Sushi</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="NIDO" evidence="9">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="AMOP" evidence="7">
        <location>
            <begin position="334"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="VWFD" evidence="10">
        <location>
            <begin position="505"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="8">
        <location>
            <begin position="721"/>
            <end position="792"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8035351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="830" mass="92914" checksum="8B8E58C1E186BA3B" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C093</accession>
    <name>A0A9Q1C093_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peroxisomal biogenesis factor 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_19582</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Peroxisome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8035799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C093"/>
    <dbReference type="OrthoDB" id="411017at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016559">
        <property type="term" value="P:peroxisome fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008733">
        <property type="entry name" value="PEX11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12652:SF50">
        <property type="entry name" value="PEROXIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12652">
        <property type="entry name" value="PEROXISOMAL BIOGENESIS FACTOR 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05648">
        <property type="entry name" value="PEX11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="1" id="KW-0962">Peroxisome biogenesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00046271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8035799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="159" mass="17916" checksum="2C5AA93249B976C8" modified="2023-09-13" version="1">MARTLDFLQKVLDALNKKDETEMLLEVLGYLMKSAWLFTDHIIWFGKIKVITIDTKQWGKNSAWCWLAANSTLAVRDMYKLQQLLHHYQELKRAGDPIPGTHLQEEIRKTKLQLVIDLCDITIPLSSLGYTSKGLGAAGGVVSSVVGGYLVWQKNVGQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C0T4</accession>
    <name>A0A9Q1C0T4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_19803</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8035961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C0T4"/>
    <dbReference type="OrthoDB" id="416119at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19446">
        <property type="entry name" value="REVERSE TRANSCRIPTASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8035961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="102" mass="11742" checksum="96227C220AA28239" modified="2023-09-13" version="1">MANNKSPGLDGYPAEFFKYFWNDIGEYIRRALNDCYKTGSLSVTLSRGVITLIPKPNKDRRYLNHWRPITLLSVIYKLASSCIANRLKKFLAVLSLLIRKGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C0W6</accession>
    <name>A0A9Q1C0W6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Aminotransferase class I/classII large domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_20010</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C0W6"/>
    <dbReference type="OrthoDB" id="7042322at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050596">
        <property type="entry name" value="AspAT/PAT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46383">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46383:SF1">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="7">
        <location>
            <begin position="57"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="11"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8036129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="162" mass="18208" checksum="777BA7316DFABBCC" modified="2023-09-13" version="1">MSTNMENQSVEGKDKNPQSHGDDFLLPHLRRYHGASNLAFNELVKKKRELGDHIYHLAFGQSPFPVMKEAQEALARNAGQAAYLDVAGLPKLREAICRFHDKYDNLSHFAAGDVVVGPGSKELIFLLMSVFSGGKCYHFVEIKKNCQIESLMMINVEKETQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C4C2</accession>
    <name>A0A9Q1C4C2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Low-density lipoprotein receptor-related protein 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_15872</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C4C2"/>
    <dbReference type="OrthoDB" id="10046193at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000241">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050778">
        <property type="entry name" value="Cueball_EGF_LRP_Nidogen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003410">
        <property type="entry name" value="HYR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000033">
        <property type="entry name" value="LDLR_classB_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46513">
        <property type="entry name" value="VITELLOGENIN RECEPTOR-LIKE PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02494">
        <property type="entry name" value="HYR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00058">
        <property type="entry name" value="Ldl_recept_b"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00135">
        <property type="entry name" value="LY"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63825">
        <property type="entry name" value="YWTD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50825">
        <property type="entry name" value="HYR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51120">
        <property type="entry name" value="LDLRB"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="547"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="9">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="7">
        <location>
            <begin position="166"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="7">
        <location>
            <begin position="209"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="7">
        <location>
            <begin position="253"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="HYR" evidence="10">
        <location>
            <begin position="374"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="HYR" evidence="10">
        <location>
            <begin position="456"/>
            <end position="536"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8038441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="627" mass="70419" checksum="40AB7C7AAD9ED135" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C4I2</accession>
    <name>A0A9Q1C4I2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF4806 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_15608</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C4I2"/>
    <dbReference type="OrthoDB" id="8887905at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032071">
        <property type="entry name" value="DUF4806"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34153:SF2">
        <property type="entry name" value="SI:CH211-262H13.3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34153">
        <property type="entry name" value="SI:CH211-262H13.3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16064">
        <property type="entry name" value="DUF4806"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4806" evidence="2">
        <location>
            <begin position="143"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8038242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="272" mass="29828" checksum="73873C2B09F49CCB" modified="2023-09-13" version="1">MDKLKACGLPGNMRRGEGSRCIPALSACMHLVPPQYQQSSAHDDGQDENPHSNSLETVPAFHSNATSSGTSSIPRSPPLAPTSNTGSNTGFQVTVIKLLTEMALEVKEVKSRVDHNTALLNDLNLQKEGMSVPEDPMDIDLDFQLPVQTVETLEEFEERLTSAEFVKKLLLKLSVLGGKDVQSTVKNLMSNLLAPSVAQQYNFTGAKGKKEFRQLELAGVILRAVRMNPRTQNAQKDDVKQALVSYLFNARDLMGGRRKRRANGQVVPATEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C4Y9</accession>
    <name>A0A9Q1C4Y9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ERI1 exoribonuclease 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Exonuclease domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HOLleu_18962</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Nanhai2018</strain>
            <tissue evidence="15">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the ERI2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8038001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C4Y9"/>
    <dbReference type="OrthoDB" id="448399at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06133">
        <property type="entry name" value="ERI-1_3'hExo_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000062">
        <property type="entry name" value="ERI1 exoribonuclease 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051274">
        <property type="entry name" value="3-5_Exoribonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047201">
        <property type="entry name" value="ERI-1_3'hExo-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Ribonucl_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010666">
        <property type="entry name" value="Znf_GRF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23044">
        <property type="entry name" value="3'-5' EXONUCLEASE ERI1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23044:SF61">
        <property type="entry name" value="3'-5' EXORIBONUCLEASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00929">
        <property type="entry name" value="RNase_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06839">
        <property type="entry name" value="Zn_ribbon_GRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51999">
        <property type="entry name" value="ZF_GRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GRF-type" evidence="14">
        <location>
            <begin position="532"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="242"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="263"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00068097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00083876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ8038001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="608" mass="67427" checksum="27CDB8419473DF43" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C748</accession>
    <name>A0A9Q1C748_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_13653</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8039607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C748"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8039607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="74" mass="8553" checksum="0007DE335F7CA88A" modified="2023-09-13" version="1">MWMCYGEKMDPIVFGGGQRSSGVTRGKKAKFSKNSQEFYMGSAEVKEQNFPRFPKIVIWSHQRSNCKFPKNSQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CA13</accession>
    <name>A0A9Q1CA13_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Reverse transcriptase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_11875</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09274">
        <property type="entry name" value="RNase_HI_RT_Ty3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="3.30.70.270:FF:000115">
        <property type="entry name" value="Polyprotein of retroviral origin, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.370:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 17.6-like protein"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="3.30.420.10:FF:000032">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 297-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon gypsy-like Protein"/>
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        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
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        <property type="entry name" value="Integrase_cat-core"/>
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    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054465">
        <property type="entry name" value="Integrase_p58-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
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        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
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    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
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        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
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        <property type="entry name" value="RT_RNaseH"/>
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        <property type="entry name" value="SCAN_sf"/>
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        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
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    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF22938">
        <property type="entry name" value="Integrase_p58_C"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF17917">
        <property type="entry name" value="RT_RNaseH"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF02023">
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        <property type="entry name" value="DNA/RNA polymerases"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
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        <property type="entry name" value="Ribonuclease H-like"/>
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        <property type="entry name" value="RT_POL"/>
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    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
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    <keyword evidence="6" id="KW-0695">RNA-directed DNA polymerase</keyword>
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    <feature type="domain" description="Integrase catalytic" evidence="9">
        <location>
            <begin position="643"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase" evidence="8">
        <location>
            <begin position="1057"/>
            <end position="1234"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="112"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="376"/>
            <end position="460"/>
        </location>
    </feature>
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        <location>
            <begin position="476"/>
            <end position="515"/>
        </location>
    </feature>
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            <begin position="112"/>
            <end position="121"/>
        </location>
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        <location>
            <begin position="127"/>
            <end position="149"/>
        </location>
    </feature>
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            <begin position="151"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="376"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="400"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="420"/>
            <end position="437"/>
        </location>
    </feature>
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        <location>
            <begin position="445"/>
            <end position="460"/>
        </location>
    </feature>
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        <location>
            <begin position="492"/>
            <end position="515"/>
        </location>
    </feature>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
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            <dbReference type="ARBA" id="ARBA00022722"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAJ8041140.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1449" mass="164671" checksum="C91DD032BBD2B10C" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CAK9</accession>
    <name>A0A9Q1CAK9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">YqaJ viral recombinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_15533</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8041044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome 6"/>
    </dbReference>
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        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="PDDEXK_lambda_exonuclease-like"/>
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        <property type="entry name" value="Restrct_endonuc-II-like"/>
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        <property type="entry name" value="YqaJ_viral_recombinase"/>
    </dbReference>
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        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="YqaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YqaJ viral recombinase" evidence="1">
        <location>
            <begin position="107"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8041044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="273" mass="30257" checksum="2FF4D799533BB433" modified="2023-09-13" version="1">MAHHGEKSIQDFYLDWLASAKNVKYGILAYVKPKADPTRKTPNPATYEEEVAVETTKFVPTTCEAFQNSIENGSVNVNKMISLLKADQHQQILLAEETKNQASSSLWVKHGADRITGSVAGDCVKSVKDNGSVSGYSHIARVMGYYGNSTSPSLNWGKNQEPIARKQYIAWHKLNYRHTGVSCTPTGLRIVSECPYVTASSDGLISRHQCGNGILEIKNPYTYSILLQLSLSFFHFSPIGIVPELTTRKIEMLSITKSVQKVISSILDKNLFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CAZ5</accession>
    <name>A0A9Q1CAZ5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Hyalin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_12507</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6132182at2759"/>
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        <property type="component" value="Chromosome 5"/>
    </dbReference>
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        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="5"/>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="10"/>
    </dbReference>
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        <property type="entry name" value="Laminin"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
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        <property type="entry name" value="HYR_dom"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24273">
        <property type="entry name" value="FI04643P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24273:SF32">
        <property type="entry name" value="HYALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02494">
        <property type="entry name" value="HYR"/>
        <property type="match status" value="46"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50825">
        <property type="entry name" value="HYR"/>
        <property type="match status" value="38"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040314793" evidence="5">
        <location>
            <begin position="22"/>
            <end position="4559"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="4524"/>
            <end position="4542"/>
        </location>
    </feature>
    <feature type="domain" description="HYR" evidence="7">
        <location>
            <begin position="18"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="HYR" evidence="7">
        <location>
            <begin position="104"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="HYR" evidence="7">
        <location>
            <begin position="189"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="HYR" evidence="7">
        <location>
            <begin position="276"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="domain" description="HYR" evidence="7">
        <location>
            <begin position="363"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="domain" description="HYR" evidence="7">
        <location>
            <begin position="447"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="domain" description="HYR" evidence="7">
        <location>
            <begin position="534"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="domain" description="HYR" evidence="7">
        <location>
            <begin position="618"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="domain" description="HYR" evidence="7">
        <location>
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</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
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        </submittedName>
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        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
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                <person name="Chen T."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain evidence="6">Nanhai2018</strain>
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        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
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    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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    </dbReference>
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        </location>
    </feature>
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        </location>
    </feature>
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        <location>
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            <end position="14"/>
        </location>
    </feature>
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        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
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            <dbReference type="EMBL" id="KAJ8043046.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
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    <name>A0A9Q1CD41_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Zinc finger BED domain-containing protein DAYSLEEPER</fullName>
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    </protein>
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    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
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        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
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            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8042787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CD41"/>
    <dbReference type="OrthoDB" id="1869581at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052035">
        <property type="entry name" value="ZnF_BED_domain_contain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46481:SF10">
        <property type="entry name" value="ZINC FINGER BED DOMAIN-CONTAINING PROTEIN 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46481">
        <property type="entry name" value="ZINC FINGER BED DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140996">
        <property type="entry name" value="Hermes dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8042787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="144" mass="16239" checksum="C2921F87CA49D007" modified="2023-09-13" version="1">MRHVPQVDRQFNKLRVQPFETKTPYYTSSREELNYFGFFVTKTVCHPTKTQKLNTTIGNTIAKDSLPVSFVEGDGFRDLMKAIEPSYTVSSRKTVSTLLLAKFEPNKTNVKTAIEVMASVSLTSDYWTSRASEGYTTVTAHGID</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CD99</accession>
    <name>A0A9Q1CD99_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_13457</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8042409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CD99"/>
    <dbReference type="OrthoDB" id="5982857at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8042409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="99" mass="11336" checksum="4EDCF3D06E99BF2C" modified="2023-09-13" version="1">MSQLGSTQNVSDSLMDACEKFVCKLYGSNVITSVNTLRFELFTRTRAKSRMLPPNKDSLTFHVKRANYQAFIWKRALEAKPKIDDPPGHGWKITGKCWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CDE5</accession>
    <name>A0A9Q1CDE5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Corrinoid adenosyltransferase MMAB</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">ATP:co(I)rrinoid adenosyltransferase MMAB</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_09774</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Converts cob(I)alamin to adenosylcobalamin (adenosylcob(III)alamin), a coenzyme for methylmalonyl-CoA mutase, therefore participates in the final step of the vitamin B12 conversion. Generates adenosylcobalamin (AdoCbl) and directly delivers the cofactor to MUT in a transfer that is stimulated by ATP-binding to MMAB and gated by MMAA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>cob(I)alamin-[corrinoid adenosyltransferase] + ATP = apo-[corrinoid adenosyltransferase] + adenosylcob(III)alamin + triphosphate</text>
            <dbReference type="Rhea" id="RHEA:56796"/>
            <dbReference type="Rhea" id="RHEA-COMP:14743"/>
            <dbReference type="Rhea" id="RHEA-COMP:14744"/>
            <dbReference type="ChEBI" id="CHEBI:18036"/>
            <dbReference type="ChEBI" id="CHEBI:18408"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:60488"/>
            <dbReference type="ChEBI" id="CHEBI:83228"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:56797"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homotrimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the Cob(I)alamin adenosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8042895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CDE5"/>
    <dbReference type="OrthoDB" id="549173at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008817">
        <property type="term" value="F:corrinoid adenosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009235">
        <property type="term" value="P:cobalamin metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1200.10:FF:000001">
        <property type="entry name" value="Cob(I)yrinic acid a,c-diamide adenosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1200.10">
        <property type="entry name" value="Cobalamin adenosyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016030">
        <property type="entry name" value="CblAdoTrfase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036451">
        <property type="entry name" value="CblAdoTrfase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029499">
        <property type="entry name" value="PduO-typ"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00636">
        <property type="entry name" value="PduO_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12213">
        <property type="entry name" value="CORRINOID ADENOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12213:SF0">
        <property type="entry name" value="CORRINOID ADENOSYLTRANSFERASE MMAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01923">
        <property type="entry name" value="Cob_adeno_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89028">
        <property type="entry name" value="Cobalamin adenosyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cobalamin adenosyltransferase-like" evidence="11">
        <location>
            <begin position="39"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00051988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00056747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00071654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00075216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU366026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8042895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="230" mass="25971" checksum="EA1AD639872D75D4" modified="2023-09-13" version="1">MIRNVLAQVLRRQKREFWTHVKMSYSSGSEKGQQPLFKIYTKTGDKGTSATIAGDRRPKDDNIFEALGATDELTSSIGLAREFCQDAQLDLEDQLVKIQCVIQDAGSNIATPRSKASPNQINVTEFDGFIIEELEQWIDTYTADLPPLKNFILPGGGKASASLHIARSICRRAERRVSPLVRDEDINPEVGRFLNRLSDYLFTVARYAADKEGKEETVYKRVRNSQKRDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CF62</accession>
    <name>A0A9Q1CF62_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SCAN box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_11619</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8044222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CF62"/>
    <dbReference type="OrthoDB" id="10000497at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF13">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCAN box" evidence="3">
        <location>
            <begin position="213"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="307"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="572"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="78"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="307"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="322"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="346"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="572"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="615"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="663"/>
            <end position="675"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8044222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="693" mass="77727" checksum="3DBC4C7BDD034DC0" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CH41</accession>
    <name>A0A9Q1CH41_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Synaptosomal-associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_07284</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Synapse</location>
            <location evidence="6">Synaptosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the SNAP-25 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CH41"/>
    <dbReference type="OrthoDB" id="19261at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031629">
        <property type="term" value="P:synaptic vesicle fusion to presynaptic active zone membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016082">
        <property type="term" value="P:synaptic vesicle priming"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15885">
        <property type="entry name" value="SNARE_SNAP25C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15889">
        <property type="entry name" value="SNARE_SNAP25N_23N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000007">
        <property type="entry name" value="Synaptosomal-associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000018">
        <property type="entry name" value="Synaptosomal-associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000928">
        <property type="entry name" value="SNAP-25_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19305">
        <property type="entry name" value="SYNAPTOSOMAL ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19305:SF14">
        <property type="entry name" value="SYNAPTOSOMAL-ASSOCIATED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00835">
        <property type="entry name" value="SNAP-25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0770">Synapse</keyword>
    <keyword evidence="2" id="KW-0771">Synaptosome</keyword>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="9">
        <location>
            <begin position="19"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="9">
        <location>
            <begin position="143"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="57"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="167"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8044514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="209" mass="23590" checksum="26AD34E899BC807F" modified="2023-09-13" version="1">MADDDEMRAHLESLQKAADMKTDESLESTRRMLQMTEESQEIGIKTLTELDQQGEKLDKIEGGLDQINSDMKEAEKNLTDLEKCCGLCTCPGSRHKNYERTENYKKGWKTSEDGQVTTSQPRVGDNRGVAKGDRSYVTRITNDAREDEMDENLGAVSDILGNLKNMAVDMGNEIDAQNNQIDRLNTKSEANKDRIEQADKRAKEILRKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CHC9</accession>
    <name>A0A9Q1CHC9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Hexosyltransferase</fullName>
            <ecNumber evidence="15">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HOLleu_08286</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Nanhai2018</strain>
            <tissue evidence="16">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>3-O-(N-acetyl-beta-D-glucosaminyl-(1-&gt;4)-alpha-D-mannosyl)-L-threonyl-[protein] + UDP-N-acetyl-alpha-D-galactosamine = 3-O-[beta-D-GalNAc-(1-&gt;3)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-Man]-L-Thr-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:37667"/>
            <dbReference type="Rhea" id="RHEA-COMP:13308"/>
            <dbReference type="Rhea" id="RHEA-COMP:13618"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:67138"/>
            <dbReference type="ChEBI" id="CHEBI:136709"/>
            <dbReference type="ChEBI" id="CHEBI:137540"/>
            <dbReference type="EC" id="2.4.1.313"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 15">Golgi apparatus membrane</location>
            <topology evidence="2 15">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the glycosyltransferase 31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="15"/>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CHC9"/>
    <dbReference type="OrthoDB" id="2139606at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008194">
        <property type="term" value="F:UDP-glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002659">
        <property type="entry name" value="Glyco_trans_31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214">
        <property type="entry name" value="BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214:SF219">
        <property type="entry name" value="UDP-GALNAC:BETA-1,3-N-ACETYLGALACTOSAMINYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01762">
        <property type="entry name" value="Galactosyl_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 15" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 15" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU363063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ8045303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="497" mass="57452" checksum="EC6940783F393E72" modified="2023-09-13" version="1">MMSFLSFSAFAAILAIIVFRSFPLHMWEEYFQTKRSDPFDIVIGVLSARHHFEQRQSLRNTWVGYIQEQSFPLSVLVKFIIGDKECPIPAEYRTSPYACTPSNLTADAISEEVIAVLSRDSPQDQGTDIPFPSRISFRVQHPIIIHRLGVFCKDGSNGLNTSVSVTLYDNFNEEDVVQVDFLPDSKGVQVGDYLFKPIESFVLPKDFEGSIIATSLSVDVRWMSGPQKPVVMETGGNVITFLQQRKWTDVATQNDVHHSPEYSLINHPLCNFIFTIHEPEELATLEEQKRENYKEWSDKIKATETHLEEENKQYEDIIRVETLDVYDNIPVKMLLFHKWLSNSVKFRYFIKTDDDCFLNIHHIIKVLTEETFPESEVWWGNFRYNWAVPHHGKWAELDYPSESFPAFACGSGNVISEDIVHWIDSNSKYLHPYQGEDVSMAIWLAAVFPTYIDDKRWICDEECHPDMLASAEHSVQEITELWKNWTNCGNPCECKLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CHX2</accession>
    <name>A0A9Q1CHX2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">DNA replication complex GINS protein PSF1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_08641</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Required for correct functioning of the GINS complex, a complex that plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS complex is a core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built.</text>
    </comment>
    <comment type="function">
        <text evidence="9">Required for correct functioning of the GINS complex, a complex that plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS complex seems to bind preferentially to single-stranded DNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the GINS complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the GINS1/PSF1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CHX2"/>
    <dbReference type="OrthoDB" id="10252587at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000811">
        <property type="term" value="C:GINS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902983">
        <property type="term" value="P:DNA strand elongation involved in mitotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11710">
        <property type="entry name" value="GINS_A_psf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21696">
        <property type="entry name" value="GINS_B_Psf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1030:FF:000001">
        <property type="entry name" value="DNA replication complex GINS protein PSF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021151">
        <property type="entry name" value="GINS_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036224">
        <property type="entry name" value="GINS_bundle-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005339">
        <property type="entry name" value="GINS_Psf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056783">
        <property type="entry name" value="PSF1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12914:SF2">
        <property type="entry name" value="DNA REPLICATION COMPLEX GINS PROTEIN PSF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12914">
        <property type="entry name" value="PARTNER OF SLD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24997">
        <property type="entry name" value="PSF1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05916">
        <property type="entry name" value="Sld5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158573">
        <property type="entry name" value="GINS helical bundle-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6 9" id="KW-0235">DNA replication</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GINS subunit" evidence="10">
        <location>
            <begin position="61"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="DNA replication complex GINS protein PSF1 C-terminal" evidence="11">
        <location>
            <begin position="144"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00045258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU368085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF05916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF24997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8045603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="196" mass="22713" checksum="758E3262F75A97D4" modified="2023-09-13" version="1">MYGDKALELILELKRAAEGSLPPYNEDGIRQVLEETSVLYDQNQADVLRESSMTGAESLLSAVQFRHTCLERNQRCLLAYLHNRLEIIKEMRWEFGSVLPPDIKNNMSEQESQWFNKYSKSLASYMRSLGEEGLDLTQDRKPPKNLFIQVRCLRDFGEFETEDGSMVVLKKNSQHFLLRSQCEPLIRQGILEHILS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CMV5</accession>
    <name>A0A9Q1CMV5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="24">Aminopeptidase NAALADL1</fullName>
            <ecNumber evidence="23">3.4.17.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="25">N-acetylated-alpha-linked acidic dipeptidase-like protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="30" type="ORF">HOLleu_06934</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="30" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="30">Nanhai2018</strain>
            <tissue evidence="30">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="22">Aminopeptidase with broad substrate specificity. Has lower activity with substrates that have Asp or Glu in the P2' position, or Pro in the P3' position. Lacks activity with substrates that have both Pro in the P3' position and Asp or Glu in the P2' position. Lacks carboxypeptidase activity. Lacks dipeptidyl-peptidase IV type activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>Release of an unsubstituted, C-terminal glutamyl residue, typically from Ac-Asp-Glu or folylpoly-gamma-glutamates.</text>
            <dbReference type="EC" id="3.4.17.21"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Apical cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase M28 family. M28B subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="30">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.17.21" evidence="23"/>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CMV5"/>
    <dbReference type="OrthoDB" id="5841748at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016805">
        <property type="term" value="F:dipeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08022">
        <property type="entry name" value="M28_PSMA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02121">
        <property type="entry name" value="PA_GCPII_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000101">
        <property type="entry name" value="N-acetylated alpha-linked acidic dipeptidase like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.930.40:FF:000001">
        <property type="entry name" value="N-acetylated-alpha-linked acidic dipeptidase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.30:FF:000002">
        <property type="entry name" value="N-acetylated-alpha-linked acidic dipeptidase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.40">
        <property type="entry name" value="Transferrin receptor-like, dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039373">
        <property type="entry name" value="Peptidase_M28B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007365">
        <property type="entry name" value="TFR-like_dimer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036757">
        <property type="entry name" value="TFR-like_dimer_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10404:SF77">
        <property type="entry name" value="GLUTAMATE CARBOXYPEPTIDASE 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10404">
        <property type="entry name" value="N-ACETYLATED-ALPHA-LINKED ACIDIC DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04253">
        <property type="entry name" value="TFR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47672">
        <property type="entry name" value="Transferrin receptor-like dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="13" id="KW-0106">Calcium</keyword>
    <keyword evidence="30" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16" id="KW-0224">Dipeptidase</keyword>
    <keyword evidence="19" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="20" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="18 26" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="8" id="KW-0645">Protease</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="9 26" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 26" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="domain" description="PA" evidence="27">
        <location>
            <begin position="165"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M28" evidence="29">
        <location>
            <begin position="351"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="domain" description="Transferrin receptor-like dimerisation" evidence="28">
        <location>
            <begin position="625"/>
            <end position="745"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00052003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00059290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00066561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00068168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00081462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="Pfam" id="PF04253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="EMBL" id="KAJ8047826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="748" mass="83637" checksum="F750F2037AD9A0F0" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CMZ3</accession>
    <name>A0A9Q1CMZ3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein DD3-3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_06769</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CMZ3"/>
    <dbReference type="OrthoDB" id="167398at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053320">
        <property type="entry name" value="Protein_DD3-3_O-glyco"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35170">
        <property type="entry name" value="PROTEIN DD3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35170:SF2">
        <property type="entry name" value="PROTEIN DD3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040332453" evidence="2">
        <location>
            <begin position="18"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="409"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="521"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="414"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="531"/>
            <end position="545"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8047708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="668" mass="75936" checksum="343E262754B2E96B" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CN27</accession>
    <name>A0A9Q1CN27_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Substance-K receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_06574</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CN27"/>
    <dbReference type="OrthoDB" id="5959154at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008528">
        <property type="term" value="F:G protein-coupled peptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007218">
        <property type="term" value="P:neuropeptide signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00637">
        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24230">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24230:SF75">
        <property type="entry name" value="RELAXIN FAMILY PEPTIDE RECEPTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="69"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="108"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="151"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="198"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="276"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="316"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="342"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="50"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8047544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="364" mass="42160" checksum="5B3F5AB0FE53213D" modified="2023-09-13" version="1">MKAHTVVGTSSLLTTEGTTSTNTVDQVSSKWEFMGVVLYYILLYSVSGIGNILSILVSRKNLSNRRKVADLLLFYLSVTDLFCLLFSHSFSLVSTFSGRWLGNKVTCIIQYYFAWSCLKMSFFILILMTFDRYLALVKPIYFRTRWNIKRATIYLVCCFLFSFGSTLLTLIIERDSIKSPEGWFLCLNSWSSDNTYDFIAVAFYGTFFAIGLILLNICNIRIAFELFKLRKRRREFSASFALASLREKKAKTTNSDDFRQLSMTSQNPESYTIKNVRISATMLVISILFVISWSPYQAYLILKLSGLDLNQRIELLALRIVFANIAFNPLLYGLFNRRYRKGYCYFLRIIFFYCLCGYITKPKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CNC9</accession>
    <name>A0A9Q1CNC9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="11">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HOLleu_06950</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Nanhai2018</strain>
            <tissue evidence="15">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase C12 family. BAP1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="11"/>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CNC9"/>
    <dbReference type="OrthoDB" id="1924260at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09617">
        <property type="entry name" value="Peptidase_C12_UCH37_BAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.532.10:FF:000002">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.860">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.532.10">
        <property type="entry name" value="Peptidase C12, ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001578">
        <property type="entry name" value="Peptidase_C12_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036959">
        <property type="entry name" value="Peptidase_C12_UCH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041507">
        <property type="entry name" value="UCH_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10589">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10589:SF28">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE BAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01088">
        <property type="entry name" value="Peptidase_C12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18031">
        <property type="entry name" value="UCH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00707">
        <property type="entry name" value="UBCTHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52048">
        <property type="entry name" value="UCH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52049">
        <property type="entry name" value="ULD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 10 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4 10 11" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10 11" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5 10 11" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UCH catalytic" evidence="14">
        <location>
            <begin position="4"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="142"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="301"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="481"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="572"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="690"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="632"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="145"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="155"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="301"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="331"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="342"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="389"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="407"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="430"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="490"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="508"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="603"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="693"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="10">
        <location>
            <position position="90"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10">
        <location>
            <position position="170"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="10">
        <location>
            <position position="84"/>
        </location>
    </feature>
    <feature type="site" description="Important for enzyme activity" evidence="10">
        <location>
            <position position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS52048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ8047840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="731" mass="83001" checksum="ED846EC6A2CF1EEA" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-04-02" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CNK9</accession>
    <name>A0A9Q1CNK9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">N-lysine methyltransferase KMT5A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_01568</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="5376140at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33480">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33480:SF1">
        <property type="entry name" value="TYR RECOMBINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SET" evidence="2">
        <location>
            <begin position="1"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="421"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="532"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="640"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1283"/>
            <end position="1355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="448"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="474"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="555"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="579"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="591"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="612"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="641"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="671"/>
            <end position="689"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8049022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1424" mass="160937" checksum="0E4FE1565EDF4A04" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CPB2</accession>
    <name>A0A9Q1CPB2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NLR family CARD domain-containing protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_00350</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CPB2"/>
    <dbReference type="OrthoDB" id="120976at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01120">
        <property type="entry name" value="RecA-like_superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312">
        <property type="entry name" value="NACHT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312:SF2">
        <property type="entry name" value="NUCLEOTIDE-BINDING OLIGOMERIZATION DOMAIN-CONTAINING PROTEIN 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NACHT" evidence="2">
        <location>
            <begin position="288"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="41"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8048159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="882" mass="100763" checksum="0C65C6A72915D71D" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CPH5</accession>
    <name>A0A9Q1CPH5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ATP-dependent DNA helicase</fullName>
            <ecNumber evidence="9">5.6.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_01094</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.3" evidence="9"/>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="4917215at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010285">
        <property type="entry name" value="DNA_helicase_pif1-like_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025476">
        <property type="entry name" value="Helitron_helicase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049163">
        <property type="entry name" value="Pif1-like_2B_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051055">
        <property type="entry name" value="PIF1_helicase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47642">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47642:SF5">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14214">
        <property type="entry name" value="Helitron_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05970">
        <property type="entry name" value="PIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21530">
        <property type="entry name" value="Pif1_2B_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 9" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="7 9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 9 14" id="KW-0347">Helicase</keyword>
    <keyword evidence="3 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="1 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helitron helicase-like" evidence="12">
        <location>
            <begin position="390"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="domain" description="DNA helicase Pif1-like DEAD-box helicase" evidence="11">
        <location>
            <begin position="1081"/>
            <end position="1251"/>
        </location>
    </feature>
    <feature type="domain" description="DNA helicase Pif1-like 2B" evidence="13">
        <location>
            <begin position="1314"/>
            <end position="1345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="285"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF05970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF14214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF21530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ8048685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1612" mass="182779" checksum="443BD3E9E996F8E2" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CQY5</accession>
    <name>A0A9Q1CQY5_HOLLE</name>
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        <submittedName>
            <fullName evidence="3">Breast cancer type 1 susceptibility protein-like</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="ORF">HOLleu_01517</name>
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    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
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            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
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        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN-RELATED"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <source>
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
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    </evidence>
    <sequence length="397" mass="44252" checksum="E239FAC788C3171B" modified="2023-09-13" version="1">MKCKMARHLKSIHAEEREVAQALSWKTNSKDRSAPWELIRKKGDFYHNTKVLQTGSGNLIVVRRSSKGNDTEADYGYVETGTRNNGREPKPDRAKTQQQTNHISQRMRQLARLVLQLRQDTGNEDSQLRDYINPERFDVVVGAVKEVSKFDQAGTTKVGVPSMALKLGHSLKKCAAIVRGIGLRSKCATTIENAGYFLELMKGTSSEEISTHALTTLGEGKFNQPEILPVTEDLLKSAVKRLAEVTGNLYTENIDTSTTHLVMKTVKDRVPDRTLKFFEAVANKLWVVAFAWVEASFKRGSLAEEEPFEVRGDTTLGPSHYGPKHAREAVEPLLQDYSISIIGHNPNISKEQVASLAKTEGAEIVKMPMESNRRKSVAVLLTDSTAPEKIDGNIFCW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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    </protein>
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        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
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            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
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            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
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        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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        <source>
            <dbReference type="EMBL" id="KAJ8049521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="354" mass="40637" checksum="271015D47D3C6BC9" modified="2023-09-13" version="1">MLYDVLFHTDSSLTEVSLFARTLPKPKAVFKRKPFFNGRLPIFNKSNTIHPQVEIKTENDRKYAKKYNENRATSKDKEISIIDLDKVEPPLNFLYEKLTLVTSFSQNHSREGLGFIGSAQQQMPNKTIIVYDLGLNEHSANQIMSFCNVQLRRFPFESYPAHLKTLKTYGFKPTIINLVLNEFGVIYYGDSSIRFKKPLSELLPDCVHHHGFLGQIRCFDPKIPRKAKYGCYHDYGFTKKEMYDKIGVNKTEYHNANFSSPAFSGGLMLVINSSSVQWKIMRPWLKCALDVTCIAPDGANLRNHRFDQSALSLIAFKNMRGEWTLENDPTPHHDTVVSVLRRSNGAKWKPKSCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CTL1</accession>
    <name>A0A9Q1CTL1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_04219</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
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        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="KAJ8050860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8050860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="114" mass="13545" checksum="925DF590B5B4067B" modified="2023-09-13" version="1">MWVQYLIDMQKLQHEKDVKLQIEKLQHDRELEIIKAEKEIELRKLEFSERSSSGSLHGNSQENLNMTYGYDVRRVPRLRDEDDIDVYLRAFELLAEGNGWDKSQWAKLLIPSKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H0F8</accession>
    <name>A0A9Q1H0F8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Little elongation complex subunit 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_30412</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
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    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <location>
            <begin position="1895"/>
            <end position="1912"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1913"/>
            <end position="1929"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1937"/>
            <end position="1946"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1954"/>
            <end position="1970"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="2464"/>
            <end position="2475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8028230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="2502" mass="279609" checksum="7B9908D963289C3F" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H1I1</accession>
    <name>A0A9Q1H1I1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_24884</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H1I1"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="173"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="45"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8031632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="300" mass="33181" checksum="CF31A49BC80A58BE" modified="2023-09-13" version="1">MDISFSSSLHSTVPQDFEGFQDRGRSKHRSSSKSKKKKRRRRHSSSSSSSPSPSSRSRKRRHSPHTDFPTEALSRLVTLLSQSADRLATPSQPAQDQPSTSITQPSSDPLLLPEHRPDSPSEVESVATGDFQFHVTGSHSHASEDSDDEPIMGTSFSKESFEKAVEVVRRQLGFDSPPVPDPPVAKRSKLSLNKPAGPRKPVMPVDTECFDRFESQANSRVWRAFPKRQSSDFHISDEDFKSFFSSPAIPDSCLDKLKSAGAVDSRGHFRSPTTKKSFTSLHGMDLAARTVCQGRSKSLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H1S2</accession>
    <name>A0A9Q1H1S2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Epoxide hydrolase 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_28143</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the AB hydrolase superfamily. Epoxide hydrolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8028891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H1S2"/>
    <dbReference type="OrthoDB" id="408373at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004301">
        <property type="term" value="F:epoxide hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43329">
        <property type="entry name" value="EPOXIDE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="12"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="AB hydrolase-1" evidence="4">
        <location>
            <begin position="82"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8028891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="353" mass="39912" checksum="659694DCC5EB6FFD" modified="2023-09-13" version="1">MGHLTRVYNALLYYFMVIFGTGLCLLITFLITLTHPLGVFQRRKRYLQKPACMDDPQLGSHGHVRTGDGKIHYVVSGPRDKPLMLFLHGFPENWYTWRHQIREFQKDYRVIAMDTRGAGESQKFHSVDSYTIDKLSLDVKAVLTELGYSSCVLVGHDWGGMIALHFAGAYPDLVDKLIIMNAADITQLHHVLYTNIKQRLKSWYMFAYQIPWLPELMVGLFVKLGLMEGGYEISEENKNITSEDAAAISYGLCLPGSLAAGINYYRANISTPNPSQIRHAILCPTLLIWGTGDAYLDVCLTQGHDKYIQDFTVSFVEGANHFVNQVKPQEVNKAMREFLQGDGSDQSSNIYIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H283</accession>
    <name>A0A9Q1H283_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_27309</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H283"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040341011" description="Ig-like domain-containing protein" evidence="3">
        <location>
            <begin position="26"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="428"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="460"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="466"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8030794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="493" mass="55125" checksum="89EC2A60C3012027" modified="2023-09-13" version="1">MYLYLTEFLGKAMYLYLLCVAFANGQRKVDVSVNGYMVVQTNVTTYTIHNPPDRIPRTLTISCLSFLGVNTTVSLLRNGSNLTDEFEHSTCTNRSLITQFKYTKENVNLLQGVYTCGFNFSSPSHNESRFEYYVESEIEINTTTTSEVCGSSVGFSGFAKEEVNLLCENSHYNLHANFSQAEQKLTSKYSLQVSLLKGHATFSCVLSYDNNLENICSHGFKVHESLNVNISPKHFTLDSHVLNFFCNSTPPRMLYWELIEDDENIFNLNSTGDLPKADGITIEIMQRAGWSSLRISTSVEGNISLGIQKVVCYAHPTGERSVAYASHVSLHENITTPSVNVCTCSPKTTTSSFDNDAEDTTKTNFSTTRNAIEQENTNPMQESSTVRQCCHNVTTNKKEEINKKEEVNKGEEINKEEEINKGEEINKWMIIAVFSLAIASFEALLLVIIAGIWTCRKCPTGDKNQSSKSGFSGGIISSETELHENPVYQPYDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H2G1</accession>
    <name>A0A9Q1H2G1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">WD repeat-containing protein 44</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_27611</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8031019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H2G1"/>
    <dbReference type="OrthoDB" id="20550at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040324">
        <property type="entry name" value="WDR44/Dgr2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14221">
        <property type="entry name" value="WD REPEAT DOMAIN 44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14221:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="511"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="618"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="658"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="319"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="465"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="558"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="893"/>
            <end position="914"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="15"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="50"/>
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        </location>
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        <location>
            <begin position="147"/>
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        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="161"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="175"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="242"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="323"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="558"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="575"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="893"/>
            <end position="913"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00021207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8031019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="936" mass="106525" checksum="50EF10EA130DF932" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H3P5</accession>
    <name>A0A9Q1H3P5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_25039</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H3P5"/>
    <dbReference type="OrthoDB" id="205623at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="30"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8031738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="155" mass="18839" checksum="F0FE14DBB04AE012" modified="2023-09-13" version="1">MNPRMNPRMNVRMNPRMDPRMNVRMNARMNPRMNPRMNPRMNTRMNPRMKDESEDESEDESEDKCEDESEDESEDSFRLEFKKTVRGLADFLDCPLTSDKLLDICAYWDEEAKYNRKEDRVGYWKYHFTIHVNEKFEKVYPDKLKDSDLTFDFDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H4B6</accession>
    <name>A0A9Q1H4B6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_21924</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H4B6"/>
    <dbReference type="OrthoDB" id="416119at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31635">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31635:SF196">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="185"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase" evidence="4">
        <location>
            <begin position="504"/>
            <end position="756"/>
        </location>
    </feature>
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            <begin position="1"/>
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        </location>
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        <location>
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            <end position="167"/>
        </location>
    </feature>
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            <end position="34"/>
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    </feature>
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        <location>
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            <end position="79"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
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            <end position="102"/>
        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
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            <end position="137"/>
        </location>
    </feature>
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        <location>
            <begin position="149"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8034902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1164" mass="133325" checksum="317D2F7A76001A78" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H5E6</accession>
    <name>A0A9Q1H5E6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Nucleolar protein 4-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_23133</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H5E6"/>
    <dbReference type="OrthoDB" id="10047222at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056549">
        <property type="entry name" value="HTH_NOL4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039788">
        <property type="entry name" value="NOL4/NOL4L"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12449">
        <property type="entry name" value="DEATH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12449:SF22">
        <property type="entry name" value="NUCLEOLAR PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23079">
        <property type="entry name" value="HTH_NOL4_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleolar protein 4 helical" evidence="2">
        <location>
            <begin position="116"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="180"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="256"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="185"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="259"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8033016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="384" mass="42557" checksum="94AD00926CBC7492" modified="2023-09-13" version="1">MTDPSIASEMETRSMNSVDTTTVSSYETPLDLKKAGEGRGSISPLSRSPAKDAGSPSPPKLQKGPNGISYSGTLQSETSKDTPEDLSVSKDDDDDDDGDDDSEKANETTPAGVDPERLKSFNMFVRLFVDENLDRIVPISKQPKEKIQAIIEACYRQFPEFHERARKRIRTYLKSCRRMKRQRDQNGIDSSTIRPTPPHLTSARAEQILAAACESESENARRMRMELVQQQQQQQLQQHTQQPQTVPVQVSGGCLGVSQAESQPDSRTAVTTQATPQPRPYEDSIPKQSPRATVGYHFENGVGGSHDPYFVPTQHQASVQPTVQHTPITNGPTDLSVKKLSSSKAQLSPTEIANIRQLITSYRESAAFLYRSADELEQMLLQLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H887</accession>
    <name>A0A9Q1H887_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">E3 ubiquitin-protein ligase LRSAM1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_17050</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H887"/>
    <dbReference type="OrthoDB" id="1711136at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16515">
        <property type="entry name" value="RING-HC_LRSAM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="1 3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="773"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="132"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="185"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="227"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="251"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="344"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="385"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="446"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="599"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="721"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="146"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="191"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="228"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="251"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="348"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="385"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="412"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="450"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="721"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="742"/>
            <end position="753"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8039357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="823" mass="94514" checksum="4FC56408BD7F8D46" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HB59</accession>
    <name>A0A9Q1HB59_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Nuclear receptor subfamily 5 group A member 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HOLleu_16673</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Nanhai2018</strain>
            <tissue evidence="15">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nuclear hormone receptor family. NR5 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HB59"/>
    <dbReference type="OrthoDB" id="5771769at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004879">
        <property type="term" value="F:nuclear receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009755">
        <property type="term" value="P:hormone-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009888">
        <property type="term" value="P:tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07167">
        <property type="entry name" value="NR_DBD_Lrh-1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06944">
        <property type="entry name" value="NR_LBD_Ftz-F1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000006">
        <property type="entry name" value="Nuclear receptor subfamily 5 group A member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.565.10:FF:000011">
        <property type="entry name" value="Nuclear receptor subfamily 5, group A, member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035500">
        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016355">
        <property type="entry name" value="NR5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001723">
        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001628">
        <property type="entry name" value="Znf_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24086:SF15">
        <property type="entry name" value="NUCLEAR HORMONE RECEPTOR FTZ-F1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24086">
        <property type="entry name" value="NUCLEAR RECEPTOR SUBFAMILY 5 GROUP A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00105">
        <property type="entry name" value="zf-C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002530">
        <property type="entry name" value="Nuc_orph_FTZ-F1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00398">
        <property type="entry name" value="STRDHORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00047">
        <property type="entry name" value="STROIDFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00430">
        <property type="entry name" value="HOLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00399">
        <property type="entry name" value="ZnF_C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48508">
        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51843">
        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00031">
        <property type="entry name" value="NUCLEAR_REC_DBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51030">
        <property type="entry name" value="NUCLEAR_REC_DBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9 12 15" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Nuclear receptor" evidence="13">
        <location>
            <begin position="70"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="NR LBD" evidence="14">
        <location>
            <begin position="271"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="495"/>
        </location>
        <ligand>
            <name>a phospholipid derivative</name>
            <dbReference type="ChEBI" id="CHEBI:16247"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="499"/>
        </location>
        <ligand>
            <name>a phospholipid derivative</name>
            <dbReference type="ChEBI" id="CHEBI:16247"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR002530-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ8039073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="520" mass="59325" checksum="0883270B264B88C7" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HBG2</accession>
    <name>A0A9Q1HBG2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_16776</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HBG2"/>
    <dbReference type="OrthoDB" id="10060997at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8039151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="139" mass="15747" checksum="7D69D0A9C0DC03BF" modified="2023-09-13" version="1">MAFEQHSSCFVPFLIMSINVSFSSGVFPESLKEAIISPIIKKLTLEKNTLSNYWPVSNIKVVAKIIEMTAADRLTDHLNRNNLTENYQSANKTFLYTETALLRVKKNDFISAIDKQQVVLLVMLDLSAALDTIRPFNFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HBU9</accession>
    <name>A0A9Q1HBU9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Ras-related protein Rab-36</fullName>
            <ecNumber evidence="3">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">HOLleu_10646</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">Nanhai2018</strain>
            <tissue evidence="19">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15">Golgi apparatus membrane</location>
            <topology evidence="15">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="3"/>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HBU9"/>
    <dbReference type="OrthoDB" id="413584at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000707">
        <property type="entry name" value="RAB36, member RAS oncogene family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0636">Prenylation</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00037794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00058763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00067830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAJ8043527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="262" mass="29390" checksum="EF8172A7AAAC8703" modified="2023-09-13" version="1">MSSSKGVNDRSISLFPEPYRPDATPYKVKDFHASVRNLASSNKTGSIGLQMSKCIVVGDVAVGKTCLVNRFCNEVFDKDYKATIGVDFEVERFEILNTPFNLQIWDTAGQERFKCIAAAYYRGAHVVIIVFDLTDKFTLEHAEVWHAEAVKEMKEAAFLFLVGTKKDICEEREFKEMEEKAVKLAKKLDAEFWAVSSLTNENVEHFFCRVASMAFNDVMKKSQEDETDGKATKKVIAADSSLISLKPETGAKDDEKKSCCSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HDA0</accession>
    <name>A0A9Q1HDA0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Dystrophin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HOLleu_15431</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Nanhai2018</strain>
            <tissue evidence="16">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <location evidence="2">Sarcolemma</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HDA0"/>
    <dbReference type="OrthoDB" id="10057795at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016010">
        <property type="term" value="C:dystrophin-associated glycoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042383">
        <property type="term" value="C:sarcolemma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099536">
        <property type="term" value="P:synaptic signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02334">
        <property type="entry name" value="ZZ_dystrophin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015153">
        <property type="entry name" value="EF-hand_dom_typ1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015154">
        <property type="entry name" value="EF-hand_dom_typ2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050774">
        <property type="entry name" value="KCMF1/Dystrophin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000433">
        <property type="entry name" value="Znf_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043145">
        <property type="entry name" value="Znf_ZZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12268:SF14">
        <property type="entry name" value="DYSTROPHIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12268">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE KCMF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09068">
        <property type="entry name" value="EF-hand_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09069">
        <property type="entry name" value="EF-hand_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00569">
        <property type="entry name" value="ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00291">
        <property type="entry name" value="ZnF_ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01357">
        <property type="entry name" value="ZF_ZZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50135">
        <property type="entry name" value="ZF_ZZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="WW" evidence="14">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="ZZ-type" evidence="15">
        <location>
            <begin position="251"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="437"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="562"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="456"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="562"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="574"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="610"/>
            <end position="625"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ8040966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="731" mass="82737" checksum="D1056CEDAE30E2BB" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HFB0</accession>
    <name>A0A9Q1HFB0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Carboxypeptidase N subunit 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_11046</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HFB0"/>
    <dbReference type="OrthoDB" id="6363818at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004180">
        <property type="term" value="F:carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050328">
        <property type="entry name" value="Dev_Immune_Receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24373:SF370">
        <property type="entry name" value="FISH-LIPS, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24373">
        <property type="entry name" value="SLIT RELATED LEUCINE-RICH REPEAT NEURONAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="398"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8043785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="438" mass="49619" checksum="2DE3EF2BE6C64C6E" modified="2023-09-13" version="1">MDMFYLFAMSKIFMMPIGLTDSVPLPQRNSNETAPICREVCEYDNWYQRARCEKRMLDKIPLPPGCESAIILELQGNNITYVDTGSLSGYRNVRTLDISNNEISKLEPETFVNMANLKNVLFSNNQLLDIQNGTFSGTENHLQRLYLNGNQISKVNEHTFSGCYAIVTLYLNNNQLEFLPATLFCGLYNMQYINLTMNHLTHIAAHTFNGLINLRFLYLAYNKISWLPNGLFTGLQSLAEVELSHNALLYIPSPDSLGVHNTFQLFDVSHNNLTETGNILPYLTIAERFHFEGNPYICDCDFLLLQKWYYNKSDTERRNYARTGPVLCHMDSLVFNIDVVIAVTSCGAVSSTRFPRFTSMHEASLTEVLVLKSAVSTSGASLNNDSKMQSITQFADKIFGVTVYFAVILTLYLALWTAKSVQSIVLQKGYDFRKGMVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HH07</accession>
    <name>A0A9Q1HH07_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_07410</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HH07"/>
    <dbReference type="OrthoDB" id="10016075at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00742">
        <property type="entry name" value="FABP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.20">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012674">
        <property type="entry name" value="Calycin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031259">
        <property type="entry name" value="ILBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11955">
        <property type="entry name" value="FATTY ACID BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50814">
        <property type="entry name" value="Lipocalins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8044623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="332" mass="35193" checksum="3E1A60F58FE8B593" modified="2023-09-13" version="1">MYFTNRYYKQANKICATERQKDHSVIMAFSGKWTVSSFEGLKAVADAFGVDEAKRDPAMLNGATVEIKQDGDNLTFTATGGLGKTGTHSMTVGQPSEGIMAFSGKWTVSSFEGLKEVAEAFGVDVSKRDPAMLKGATLEIKQDGDTLTYTTTGGLGRTGTHTMTVGQQSEGELFGKVYSATTEWDGDTIVSKGQSGLVLRRVITSKMAFNGKWTVSGFEGLNDVLDAFGVDASHRDPAKLQGATVEIKQDGDNFTFTTTGGLGKSGSHTMTVGGPSEGELFGKAYSGTTAWDGDTVVFTGKSGVVLKRSIEGANLVYTVEYGGKTGKVTFSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HHH6</accession>
    <name>A0A9Q1HHH6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_03226</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HHH6"/>
    <dbReference type="OrthoDB" id="10055248at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33480">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33480:SF1">
        <property type="entry name" value="TYR RECOMBINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="172"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8050142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="797" mass="90002" checksum="47E43A05F2970914" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HHI4</accession>
    <name>A0A9Q1HHI4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Dolichol-phosphate mannosyltransferase subunit 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_05570</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Stabilizer subunit of the dolichol-phosphate mannose (DPM) synthase complex; tethers catalytic subunit to the ER.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the dolichol-phosphate mannose (DPM) synthase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Endoplasmic reticulum membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the DPM3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HHI4"/>
    <dbReference type="OrthoDB" id="2014333at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033185">
        <property type="term" value="C:dolichol-phosphate-mannose synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013174">
        <property type="entry name" value="DPM3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16433">
        <property type="entry name" value="DOLICHOL-PHOSPHATE MANNOSYLTRANSFERASE SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16433:SF0">
        <property type="entry name" value="DOLICHOL-PHOSPHATE MANNOSYLTRANSFERASE SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08285">
        <property type="entry name" value="DPM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8046784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="94" mass="10577" checksum="52FF06C28DE7402B" modified="2023-09-13" version="1">MATKLVQWLTVFTLFTSVWVSLVFKLIPVQLDDKVLEVLWPLPVYLLVVFGSYSLATIGYRVATFNDCEDAAASLKAEIEEAKADLTRKGFKFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HJV9</accession>
    <name>A0A9Q1HJV9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_06907</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HJV9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8047806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="52" mass="5931" checksum="AE89CBC86A91E81E" modified="2023-09-13" version="1">MGEGAKGVQWADPFLGGFKYRNGGMRLDKLLEAMQMVWQKKCITTTLVTYFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HKH6</accession>
    <name>A0A9Q1HKH6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Zinc finger CCHC domain-containing protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_03901</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HKH6"/>
    <dbReference type="OrthoDB" id="2286764at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002218">
        <property type="term" value="P:activation of innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042509">
        <property type="entry name" value="ZCCHC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22639">
        <property type="entry name" value="GAG-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22639:SF3">
        <property type="entry name" value="ZINC FINGER CCHC DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="2">
        <location>
            <begin position="51"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8050624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="178" mass="20715" checksum="285BC9060F8BC326" modified="2023-09-13" version="1">MRWVTYQEGELKGILNGKRQFRMVLKREIPSFIFIGGSKAHIRYFGQPRTCFKCSEEGHEAKSCPNRRCGKCLQVGHEKAECPNEVRCNLCGGGRDMFLVPAPLRTLPVPVRMWGRHMNPRSQAQNLWWRNLGPLHLMRWHIRWSRISLLLNNVKGSLGPLRQALHRPPLQHLGRLDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HKS7</accession>
    <name>A0A9Q1HKS7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SCAN box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_04384</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HKS7"/>
    <dbReference type="OrthoDB" id="6379402at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF13">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCAN box" evidence="3">
        <location>
            <begin position="137"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="286"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="45"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="286"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="329"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="354"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8050984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="415" mass="48176" checksum="DCEC1D77354C42ED" modified="2023-09-13" version="1">MRKDEIKRYVLEYLVDESILPRTVLETAITVPTNNTFKLKRLEMEMNKEVRLKEMEREMQKEKEEREMQMEENVEKFFNHFEKLGEQLKWPRDNWSILIQSNFTGKAQEVYSALSIEDSMDYDKVKKAILQAYELVPEAYRQQFRKYLKADTQTYVEFAYQKERHFDRWCASKKVSTFDALRQLMLVEEFKRCVNDDIKTHLEENKADKLSEVANLADQYALTHKFSKVGQTQNKGNDLAGGKVVANAILTDKPCDYNNTEQLEKEFPNLFPACAVTRAMSQKLAMDDNLKHPPSKSEDTVPKSGTSRESCKDDNLKHPLSKNEGMISENKDHKLNHPPSKIEELVPKNGTTLPKEESESGKDSSKNIYESEDSCLVVIRSWNQFLRYSTLQVYYCTIVNLHALPALGPDQANIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HL33</accession>
    <name>A0A9Q1HL33_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ATP-dependent DNA helicase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_01768</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HL33"/>
    <dbReference type="OrthoDB" id="6141723at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8049153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="142" mass="15986" checksum="C3221ACAB0CACB23" modified="2023-09-13" version="1">MKSQLAPNTEHREEQDRLEGSADCEELSFYSPQQPNHQKYDTSADLGVINSPSIEVTTFCPRIPDNEFLALLTILNKKQREFYIHVMQSITSMESPLHVFLTGGAGVGKSLVVKVLYQSLHRYHNSIEGTDPDDSSAPAMRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HLA7</accession>
    <name>A0A9Q1HLA7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_03335</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HLA7"/>
    <dbReference type="OrthoDB" id="120976at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24407">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24407:SF14">
        <property type="entry name" value="SIR2-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8050220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="362" mass="42500" checksum="6A2A3851E6E90957" modified="2023-09-13" version="1">MTDVMKMIMTPVFHLFEVVLTTRYLPKHYAPNTKRLRLTGFDDRARDEYIRKAVVGANVVAANRIKQRLHENPILEDLCQVPLFFVMFAHMSHENKDFQTFKTVTSFFEYMIKCFHSHMRNKMEDKNVQRYHMFEDHHSELDEVAFEGLSKRNQQIVWKKRELITKLGKAFYDQYIRIGILVEEEVIDDIYGSDSASTDKYQREVRFYHKVFCEWYAAHYLAKYAARMPDKLSRVLRYMDPFDFQYCYRFACGLNNNAASGVIEYVKKRDGGDKFAILCILEQGDPDILDAVLSLCSEQINFSMDDNTLLQRSTIQLLEVASSHKPCHYSLVEPVPTLQHAAGLLLCHRRRACTTNPWTRPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HME3</accession>
    <name>A0A9Q1HME3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_04483</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8051058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HME3"/>
    <dbReference type="OrthoDB" id="10473521at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8051058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="104" mass="11885" checksum="4648A5F59C0858E7" modified="2023-09-13" version="1">MCEHRQYLELGKSGIISCSFQEGFFGVFWFNTTNYRSEEPILHFTQSSKTGVGYTSGEYDVLENGSLVIINVTFQHETSFTVAYFQSQRDAALYYDVKVVVLGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0Y992</accession>
    <name>A0A9Q0Y992_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Splicing factor 45</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_43843</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000494">
        <property type="protein sequence ID" value="KAJ8018263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0Y992"/>
    <dbReference type="OrthoDB" id="5411533at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000380">
        <property type="term" value="P:alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000467">
        <property type="entry name" value="G_patch_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040052">
        <property type="entry name" value="RBM17"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13288:SF8">
        <property type="entry name" value="SPLICING FACTOR 45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13288">
        <property type="entry name" value="SPLICING FACTOR 45 SPF45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01585">
        <property type="entry name" value="G-patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00443">
        <property type="entry name" value="G_patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50174">
        <property type="entry name" value="G_PATCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G-patch" evidence="2">
        <location>
            <begin position="294"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="335"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="16"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="164"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="335"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8018263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="383" mass="42383" checksum="1B1ABD5E7093FBC8" modified="2023-09-13" version="1">MSLYDGLDVDTDSKKNVSSSSNKPDVSGWSAGIRMMQSQLRLKKAALTEAQRKIRGATLAPVVDLKKGKGEDETRLPSPRNQMSPRRPVHGPPRTVKPSPFVAGVIGDITGDVTDEYDPAYPNEYEKMQRKVREKRQRESDNAWEKGRGKEDRYSNITWRRRDRGRDRDRDRDRHRDRDRSPQGFSRKPDSDEEEERDKTRRRGKQGAAIAPPSSLLNESEPPKDSFVAPAPIPKSRPYASILKKQENDPERSTTPPPGSEFSEMEDMPEMPGAPKSSLAQGPGGFSIPVGMGADSVAAKIMAKYGFKSGRGLGKKEQGISTALVVEKTSRRGGKIIEHGKDVDIDMPAPSAMPLPKQPTPASELSEAMKNPTKVVLLRVSDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YAP9</accession>
    <name>A0A9Q0YAP9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ZU5 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_44165</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000681">
        <property type="protein sequence ID" value="KAJ8018056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YAP9"/>
    <dbReference type="OrthoDB" id="6065502at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005042">
        <property type="term" value="F:netrin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.220.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037936">
        <property type="entry name" value="UNC5A-D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000906">
        <property type="entry name" value="ZU5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12582">
        <property type="entry name" value="NETRIN RECEPTOR UNC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12582:SF41">
        <property type="entry name" value="UNC5C-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00791">
        <property type="entry name" value="ZU5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ZU5" evidence="1">
        <location>
            <begin position="12"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8018056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="378" mass="43934" checksum="C365CD1C763BB124" modified="2023-09-13" version="1">MCPPNSQVLPRLTPLILCEPSGLEFIIPVKLTVPHCAVIQEPDHHKVTVEIGHKNRSMKGWTWTEETVAYSLTRKTCSVFLRHFSGVNVNIDSTEEQMLRVIPYAKLTPHSNNVLLTLWFCNDLEADFQSVMKCGENDSLVQLDSYDTFKLRHVKGAESVKVTLKDASNQWFQKEFKDCIDVNSLAGENQLYRDFRLPTVSDQDNGTYIYLDLRVTQRVGEKESETSFTLRKRVSDLRKPVCLRRLYSPCENEERILSFQEYPTDRCFAVPYIDPMRDHEDDVILTLWLCPFESQREKLQEIETKRIQYGSRAKFQFIGKKEDKDEIKFINLEVHTFQDENHRTPFIEEVDVSLLLYESKHTESSNTEDLSGVHQGIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YBN5</accession>
    <name>A0A9Q0YBN5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">OTU domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_43351</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000280">
        <property type="protein sequence ID" value="KAJ8018579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YBN5"/>
    <dbReference type="OrthoDB" id="6626362at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22755">
        <property type="entry name" value="OTU_CeDUB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003323">
        <property type="entry name" value="OTU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050704">
        <property type="entry name" value="Peptidase_C85-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12419">
        <property type="entry name" value="OTU DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12419:SF7">
        <property type="entry name" value="OTU DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02338">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50802">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OTU" evidence="2">
        <location>
            <begin position="109"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8018579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="266" mass="29833" checksum="675E4834C89336AF" modified="2023-09-13" version="1">MPKTKKASYRRRNKANKGRMQQRRLRNNACNIPVSRVIQNNGSVTKRSHEIEVIAANTGSTFHFFPVNDTWSDSMFHNLSAVSSLAQSPSINQTAIGNPQICHITAKPSVVKNIEGDGICFFRCLSYLITNSEENHLALRHQIVQSIQPTHLTSSSQTVQQYIHASNMDFPNVWSTEVEIMAAASLLQTDIYTYALQGNQWKRLRYPATGNLGTPVDKTKRAIYIVNTSSIHYDVIFAVNDSTSPTSDTHHIPSCKTTQLVKEEID</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YCF8</accession>
    <name>A0A9Q0YCF8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ficolin-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_42836</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000144">
        <property type="protein sequence ID" value="KAJ8018911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YCF8"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00087">
        <property type="entry name" value="FReD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050373">
        <property type="entry name" value="Fibrinogen_C-term_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025615">
        <property type="entry name" value="TILa_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040941">
        <property type="entry name" value="GGGWT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143:SF327">
        <property type="entry name" value="FI21813P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143">
        <property type="entry name" value="FIBRINOGEN/TENASCIN/ANGIOPOEITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12714">
        <property type="entry name" value="TILa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00186">
        <property type="entry name" value="FBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="110"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="3">
        <location>
            <begin position="146"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8018911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="391" mass="44789" checksum="1DC213BFB06F8D3B" modified="2023-09-13" version="1">MTLSNGSSFYVKYNYFRISDEWNDYAIVSVGEFRSNWTCVVSTCPLTMNRNTCTCQDVCNDPIGQTDCYTECVETCVPEGCSVPETNSLITNGESFINANCSQNCTCIDNQLNCNIHYQCSPYATCSTRDEIRKCYCNEGYEGNGETCISVFKDCYEAYQAGHTTDGIYTILPTDWPGSPFRVFCNMTFGDGGWTVFQRRIDGVTDFFRNWTEYKQGFGLLNEGNDFWLGNEQLHYLTDQKNYILRVDFVHSSDRLYYNEFTTFKIGDESTKYLMSYSSFKSGNTGYTYTFSYNNGKQFSTRDQDNDECSNHHFADRHRGGWWYSDNWCTGCYGSHCHHFQYGSSGCYTLGTYTNLNGDYNGGSAENIFYNTNGSNDCNHKFAEMKIRPSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YDI6</accession>
    <name>A0A9Q0YDI6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Leucine-rich repeat-containing protein 15</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_40357</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YDI6"/>
    <dbReference type="OrthoDB" id="1099686at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050328">
        <property type="entry name" value="Dev_Immune_Receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24373:SF398">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING G-PROTEIN COUPLED RECEPTOR 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24373">
        <property type="entry name" value="SLIT RELATED LEUCINE-RICH REPEAT NEURONAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040285316" evidence="5">
        <location>
            <begin position="31"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="631"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="domain" description="LRRCT" evidence="6">
        <location>
            <begin position="511"/>
            <end position="558"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8020698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="703" mass="79103" checksum="F4FE453DA3A8E89B" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YEC8</accession>
    <name>A0A9Q0YEC8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_42971</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000175">
        <property type="protein sequence ID" value="KAJ8018824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YEC8"/>
    <dbReference type="OrthoDB" id="10068277at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019073">
        <property type="term" value="P:viral DNA genome packaging"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003389">
        <property type="entry name" value="Adeno_IVa2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02456">
        <property type="entry name" value="Adeno_IVa2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1048">Host nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="115"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="133"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8018824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="314" mass="35094" checksum="DB02F9EE30FD4CA9" modified="2023-09-13" version="1">MNPKLAHPFTCVIAGPTGCGKTQFVKKMLTQDVIEGAPERIVYCYGEYQPAFVEMSAALPQIRFVEGLPTNLDEYLDPSYKTLIILDDIMTSHVQLYNDTLQRYLTEDKKMTGQPIGLNISTSSDNKGDKSSDNNTSTITTTTTNSNEGEALKADQILTNFPKSLKNKAKLMLNRITDHRQSGKSPIIDWNGNGELLYKGDTVKGSNLTDLILDVMHTRKDFNPVGCQKFIHGLSELNFPEAHVGNLARRQVMHQIRETGLHGDISKLLPTPPRDVRSKTGAIAKSKRRKIPNFDKVLCTCHKNWNYIKGTSKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YFL5</accession>
    <name>A0A9Q0YFL5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Beta'-coat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">HOLleu_38794</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">Nanhai2018</strain>
            <tissue evidence="19">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">COPI-coated vesicle membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat COPB2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YFL5"/>
    <dbReference type="OrthoDB" id="2150324at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030126">
        <property type="term" value="C:COPI vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22947">
        <property type="entry name" value="Coatomer_WDAD_beta-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.470:FF:000001">
        <property type="entry name" value="Coatomer subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000008">
        <property type="entry name" value="Coatomer subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006692">
        <property type="entry name" value="Beta-prop_COPA/B_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050844">
        <property type="entry name" value="Coatomer_complex_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016453">
        <property type="entry name" value="COPB2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048270">
        <property type="entry name" value="PNMA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056176">
        <property type="entry name" value="TPR_COPA_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19876">
        <property type="entry name" value="COATOMER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19876:SF2">
        <property type="entry name" value="COATOMER SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04053">
        <property type="entry name" value="B-prop_COPA_B_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14893">
        <property type="entry name" value="PNMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23953">
        <property type="entry name" value="TPR_COPA_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005567">
        <property type="entry name" value="Coatomer_beta'_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="8" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="6 14" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Paraneoplastic antigen Ma-like C-terminal" evidence="17">
        <location>
            <begin position="26"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="146"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="230"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="273"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="316"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="360"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="COPA/B second beta-propeller" evidence="16">
        <location>
            <begin position="454"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="domain" description="COPA/B TPR" evidence="18">
        <location>
            <begin position="729"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1018"/>
            <end position="1077"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="1021"/>
            <end position="1046"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="1047"/>
            <end position="1077"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00032920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF04053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF14893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF23953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAJ8021550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1077" mass="121468" checksum="29D04D1C01D06B17" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YFX5</accession>
    <name>A0A9Q0YFX5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Solute carrier family 22 member 6-A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_39384</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8022013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YFX5"/>
    <dbReference type="OrthoDB" id="10021984at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17317">
        <property type="entry name" value="MFS_SLC22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24064">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="18"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="121"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="152"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="175"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="210"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="237"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="373"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="404"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="433"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="463"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="499"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="526"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="18"/>
            <end position="551"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8022013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="585" mass="64520" checksum="C348A99A72F727D9" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YJ04</accession>
    <name>A0A9Q0YJ04_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_37256</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YJ04"/>
    <dbReference type="OrthoDB" id="1608002at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8022376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="94" mass="10795" checksum="6D1743942B695F5F" modified="2023-09-13" version="1">MPKVRLFNIYTAITRTIAVYVILQLQVITHMYITRGLCCFIPQVALPLRQRLLSLPKIGWKTVSATMSYSITERGAPNSLDYRAYFKKCIQHGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YK74</accession>
    <name>A0A9Q0YK74_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_36696</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8024068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YK74"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8024068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="83" mass="9356" checksum="08D79C2B934AF879" modified="2023-09-13" version="1">MTTATYKDDFNNEYFSNNASHTPTPNLTSIFLPFDIPLVMYFHAMFLPILQNPPLGGLFQNCTLKKATKLDKTSSLPYSAHLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YKW7</accession>
    <name>A0A9Q0YKW7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_39485</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8022092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YKW7"/>
    <dbReference type="OrthoDB" id="418169at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8022092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="286" mass="31833" checksum="0C1564D563C0387D" modified="2023-09-13" version="1">MLPDLRSFKNQGIVGRFVVNRDALEREIESRKQQDGVCGEDVKIQLPKSLSHSSYHASYQGEVLVIYLTITDNTLAAELSDDLKELVSTSYKVILVLLPIVIDMTDENIVLFLRHFSNSTKKTTYPYGTNTSELIQVDSIEEADDGTVTIRLGDDVMVYQVQDDVKYNSETKVGKDFDKVTGEVIKITNYPLSFDWGCGLFGSQVSFCEILDGVQCEIARVGGDVCTVTTTTISERSPATMKIVNMTGVFREMIPFLAETVPNESFEAELIDRQHSTGIVYPCLAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YL86</accession>
    <name>A0A9Q0YL86_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_35769</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YL86"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="261"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="268"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8023355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="423" mass="47480" checksum="5AE5259BE925A9FC" modified="2023-09-13" version="1">MDEQLIPSIPTQSDDEMEFQLVSRNKRRRTGPASPEPAQKEPEHHCKHPVILTGLPTDFRNPIAIRRYIEDSHPTAQVIGIRTSRGGLTIIAAQNATSQKELLGNWKPIRGRTPTARLPKEKKEERPGPIEGVIVGLHPEITAEEVVEELQGNANRLDPCSFRWFYRKGTKTPTWKGAVTFKTQEDLDRAVTRGVTIGFQHHRRFGHFAANCNHRKTCLRCAGHHALADCPTPRDSPKCANCRRSHIASYRGCSKFVRATEEATAKKAPPPPKTPGPPRPSPPVTNSNSVSSYLPQITETLEKNQQVTLTKMEERHQLQLEALRLQHQATIERIEEANGQLFHQLREDTEAQLNELKGKITHFLGDVLTTLIPQKGDTAPSDLRKATVIKEKAWLHLGLAIDISRIREELLKKSPTGKKTSSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YN36</accession>
    <name>A0A9Q0YN36_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_39234</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YN36"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8021901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="111" mass="12438" checksum="CFAA5E47860144F4" modified="2023-09-13" version="1">MDDHPTAKCDVENGMAKEEEELLDLVVDDSGGTLTNNWYTEHASLYEQNKQFYHYQEECLKVFKEYVTQRDCSIIDFAMGTGIMGDLVSDGAGSGDGCNHDDDMRLTVTTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YNK2</accession>
    <name>A0A9Q0YNK2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Vitamin D 25-hydroxylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_35943</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YNK2"/>
    <dbReference type="OrthoDB" id="1055148at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000036">
        <property type="entry name" value="CYtochrome P450 family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050182">
        <property type="entry name" value="Cytochrome_P450_fam2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300">
        <property type="entry name" value="CYTOCHROME P450 508A4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300:SF417">
        <property type="entry name" value="CYTOCHROME P450 508B1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0349">Heme</keyword>
    <keyword evidence="5 7 8" id="KW-0408">Iron</keyword>
    <keyword evidence="3 7 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 8" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="7">
        <location>
            <position position="372"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8023487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="426" mass="48187" checksum="0FC6BC4CA59236D2" modified="2023-09-13" version="1">MTVEVGGQTAVVLNDYDLIKSCYQSNIFNGRPKLCAFETAFGLGAGIGTGQHLHWREQRRLAVSTLRTIQFGEGITMAGIVEAEAGKLLDDLAQHKGRPLNLTHILPKAVCNIVCAFVFGKPFEYSDPRYILMLWLVYQNFKLAGPSAVVYSSGFLAKLPFGPGKRLVDNLRAFKQFAEDHIAEHRESFDGAHIRDFIDAYLLKIEESRTGSMEESEVYKHLNLVWCVNDLFGAGTETTAGTLRWAFLYMVLYPNVQKRIQDELDCVVGRGRMPVLRDKENLPYVEAAIQEINRHADIGPMGVAHATTEETQLLGYTIPKGTLTMANAHLCHFNEEYFENPRQFNPDRFINSDGSFIKREEVIAFSIGKRTCPGEELARMELFFFLTHILHRYEVTIAEGSPRPTLEGYTSITRVPHPYMVQLKER</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YPQ5</accession>
    <name>A0A9Q0YPQ5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Endonuclease-reverse transcriptase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_35440</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8025275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YPQ5"/>
    <dbReference type="OrthoDB" id="10059790at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47027:SF20">
        <property type="entry name" value="REVERSE TRANSCRIPTASE-LIKE PROTEIN WITH RNA-DIRECTED DNA POLYMERASE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8025275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="172" mass="20684" checksum="CA490A5DEEE0FADD" modified="2023-09-13" version="1">MRMNTRSDQKLKINNEDVEDVHEFTYLESKLNKSGGGSEDTKHRLAKAWSSFRKLTKICYQENIQRRTKIPLYKSLVRSVLLYGCETWKLTQHEERKLDTFQYKCLKTILRIRFPQTISYEEMEDVIKLEGMSHEIRRTRWNWIGHVLRKGNKNDTFIVVEWRPEGTRCRKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YPR5</accession>
    <name>A0A9Q0YPR5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ADP-ribosylation factor-like protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_33031</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YPR5"/>
    <dbReference type="OrthoDB" id="2011769at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04155">
        <property type="entry name" value="Arl3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000281">
        <property type="entry name" value="ADP-ribosylation factor-like protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044612">
        <property type="entry name" value="ARL2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45697">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-LIKE PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 11 13" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0519">Myristate</keyword>
    <keyword evidence="5 11 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="24"/>
            <end position="31"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="126"/>
            <end position="129"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ8025466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="181" mass="20315" checksum="FF184CCA4D1D4CBD" modified="2023-09-13" version="1">MGLLELLRKLKASPSHEIRVLLLGLDNAGKTTILKSLAGEEVSKVTPTQGFNIKSVKQEKWNLNVWDIGGQRNIRPYWRNYFENTDVLIYVIDSADRKRLDETGLELTELLEEEKLQGVPLLIFANKQDLLSAAPPGEIADSLGLNDIRTRTWQIQACSAMSGEGLEHGMNWVLKTCSKPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BAY8</accession>
    <name>A0A9Q1BAY8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_41817</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8020000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BAY8"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8020000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="79" mass="9147" checksum="EA42875436E1A87F" modified="2023-09-13" version="1">MIQKFTGKSCSTGEEAACLQNDLCKLVDWRKDWQMLFNSSKCKRVIVDWNSLPPEAVEAKTVLQFKRITGPLFKKLAYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BB71</accession>
    <name>A0A9Q1BB71_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">BRCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_43261</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000250">
        <property type="protein sequence ID" value="KAJ8018649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BB71"/>
    <dbReference type="OrthoDB" id="6496131at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRCT" evidence="1">
        <location>
            <begin position="61"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8018649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="503" mass="57530" checksum="66F22DBBC5182339" modified="2023-09-13" version="1">MYKRISYAVAKGDGSACGMKATEAMNEHIKQHLRSSGIPQGIKATDVEKYVSVSVSMDQKEDDGIFHTVTFSLCGAIPRIDGQNYSHNSFQALVKSESGKIKDKLPAVCDSTKTCIALFNPNIASKNNLPSAVTLSMKAGFPILDYKFVIECIKEKHLLDMDKLKVDLSRCSLKVIRQPSLENKLFRRTHVMTSIIKKRSMKRRRKPKLLRAKECNPAFHFVWSKIKELKVSRKEARLLMPQYFQQWKMMSRNHSNLYRRHCENLRCTVGTHVNFRVKNPAYELGMVDGKTPQNPPFVVQEEILLHNVEYQLTGAVVLRPGHFISVATFGAYIKMRNIPNHEHPCRLSQKEISHFRVFTGQTVKASCKVYVDVCYGNYEGKLKLYEMDCTEYYLLGREWLAVIPLDWKSLISQRPKAEKVLNVSELEQVINKHAELFEKGLSRLTDAKIILREEAQPVVRKPVKVTYALKEKVEVELVRLENCGVITPVSHSEWDSSIVAVEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BBB0</accession>
    <name>A0A9Q1BBB0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_43850</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="KAJ8018257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <location>
            <begin position="97"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
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            <begin position="104"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
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    </evidence>
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        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAJ8018257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="921" mass="104963" checksum="02B2ABEE1A83812E" modified="2023-09-13" version="1"> </sequence>
</entry>
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        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
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        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
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        </subcellularLocation>
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    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="KAJ8018467.1"/>
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        <location>
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            <end position="185"/>
        </location>
    </feature>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51031"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAJ8018467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="211" mass="24047" checksum="AF5EE3487D1FCFBD" modified="2023-09-13" version="1">MKKRREVKSNKSGQAAERAKKWHFFDVLSFLDTFIEENNTDSNVPFFESGCDISLEDELYSGSLTASTSEANCPDLPPSPTMCDDAESSVTPKHSNKCEETPIKLKSKRQKREKESGLEVELQGLIEQHRRTWEDVEKDTTEPRAKDGDELFFQSCTQRMKLLKPSLRSYLKMQISQLFFNAENPDAEPMSIMPLPPQMATTIFTIEAPES</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BCR3</accession>
    <name>A0A9Q1BCR3_HOLLE</name>
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        <submittedName>
            <fullName evidence="10">Vinexin</fullName>
        </submittedName>
    </protein>
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        <name evidence="10" type="ORF">HOLleu_37918</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
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            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
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        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022896.1"/>
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        <property type="evidence" value="ECO:0007669"/>
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        <property type="match status" value="3"/>
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            <begin position="149"/>
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        </location>
    </feature>
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    <feature type="compositionally biased region" description="Pro residues" evidence="6">
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        </location>
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            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022949"/>
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            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50002"/>
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        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
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        <source>
            <dbReference type="PROSITE" id="PS50831"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAJ8022896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1240" mass="137264" checksum="859ECC1DB30BD37D" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BD31</accession>
    <name>A0A9Q1BD31_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_35637</name>
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    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
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            <taxon>Aspidochirotacea</taxon>
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            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BD31"/>
    <dbReference type="OrthoDB" id="8945208at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09274">
        <property type="entry name" value="RNase_HI_RT_Ty3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.370:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 17.6-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon gypsy-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000269">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041577">
        <property type="entry name" value="RT_RNaseH_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF15">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17919">
        <property type="entry name" value="RT_RNaseH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="2">
        <location>
            <begin position="398"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="615"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="739"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="821"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="615"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="752"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="832"/>
            <end position="879"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8023271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="944" mass="106132" checksum="920138A693C4B211" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BD79</accession>
    <name>A0A9Q1BD79_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Storkhead-box protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_37265</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BD79"/>
    <dbReference type="OrthoDB" id="10020110at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040126">
        <property type="entry name" value="STOX1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22437:SF0">
        <property type="entry name" value="FI21431P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22437">
        <property type="entry name" value="WINGED HELIX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8022385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="130" mass="14776" checksum="FA1232C540A2FEE9" modified="2023-09-13" version="1">MEKVKSPVLAPDCVAVVFTTTAPKNVEKLDTTSSPGRKIRNGKDLFKELVRENKHSVWNKTLSEAVRGITFVGFLEPWTVLVKGSEKHLETLRDAYARRILKPPRNFAIVQFGKDLMLDHNKVWTKVDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BDS5</accession>
    <name>A0A9Q1BDS5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_38969</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BDS5"/>
    <dbReference type="OrthoDB" id="10366543at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="77"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="120"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="431"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="149"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8021693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="574" mass="64251" checksum="BEB046F61D808A9D" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BDX0</accession>
    <name>A0A9Q1BDX0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Mitochondrial 2-oxoglutarate/malate carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HOLleu_39113</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Nanhai2018</strain>
            <tissue evidence="18">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(S)-malate(in) + 2-oxoglutarate(out) = (S)-malate(out) + 2-oxoglutarate(in)</text>
            <dbReference type="Rhea" id="RHEA:71587"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>maleate(in) + 2-oxoglutarate(out) = maleate(out) + 2-oxoglutarate(in)</text>
            <dbReference type="Rhea" id="RHEA:71599"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30780"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>malonate(in) + 2-oxoglutarate(out) = malonate(out) + 2-oxoglutarate(in)</text>
            <dbReference type="Rhea" id="RHEA:71591"/>
            <dbReference type="ChEBI" id="CHEBI:15792"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>oxaloacetate(in) + 2-oxoglutarate(out) = oxaloacetate(out) + 2-oxoglutarate(in)</text>
            <dbReference type="Rhea" id="RHEA:71603"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>succinate(in) + 2-oxoglutarate(out) = succinate(out) + 2-oxoglutarate(in)</text>
            <dbReference type="Rhea" id="RHEA:71595"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BDX0"/>
    <dbReference type="OrthoDB" id="448427at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000013">
        <property type="entry name" value="Mitochondrial 2-oxoglutarate/malate carrier protein-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050391">
        <property type="entry name" value="Mito_Metabolite_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45618">
        <property type="entry name" value="MITOCHONDRIAL DICARBOXYLATE CARRIER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0050">Antiport</keyword>
    <keyword evidence="8" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="9 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 16 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 17" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="16">
        <location>
            <begin position="10"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="16">
        <location>
            <begin position="104"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="16">
        <location>
            <begin position="204"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00036491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00040264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00050120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00050291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00052538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00052710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ8021813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="305" mass="33140" checksum="8563BDAF42696BC3" modified="2023-09-13" version="1">MAQQPIPKPVKFLFGGTSGMAATFFVQPLDLVKNRMQMSGEGGAARAHKSSIHAIKNIVSNEGFLALYNGLSAGLLRQATYTTTRLGIYTTLFDMVSGPEGQPPSFILKATIGMTAGAVGAFVGTPADLSLIRMTSDGRLPPDQRRGYTSVFNALARIVKEEGFTTLWRGCGPTIARAMVVNAAQLASYSQAKQFLLGTGYFKDNIGCHFGASMISGLVTTIASMPVDIAKTRIQNMKIIDGKPEYRGSLDVIITVARKEGVYSLWKGFTPYYSRVGPHTVIFFIILEKSYALYRKYIMGEQTTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BK52</accession>
    <name>A0A9Q1BK52_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Alpha galactosidase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_30207</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BK52"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041233">
        <property type="entry name" value="Melibiase_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17801">
        <property type="entry name" value="Melibiase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha galactosidase C-terminal" evidence="1">
        <location>
            <begin position="71"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF17801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8028074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="152" mass="17114" checksum="CCD7F0B9928DDCE0" modified="2023-09-13" version="1">MRPLDGRWAVCFLNRGTSVPETISVPIPKLGLPSDKELGFDALDLFSGEYIPFMDSYASFNSTVNPNGIDTSEVWVKKLGLYDDWAIVLFNRGYTNATVTLRLADIGFQYVLGYELRDLFKHTEIGHVEILQYKNYTVPATGVLMAYAYPSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BKB7</accession>
    <name>A0A9Q1BKB7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA-directed DNA polymerase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_30182</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BKB7"/>
    <dbReference type="OrthoDB" id="5871067at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33568">
        <property type="entry name" value="DNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33568:SF3">
        <property type="entry name" value="DNA-DIRECTED DNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8028055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="197" mass="22866" checksum="01AD9562CB435085" modified="2023-09-13" version="1">MVCDGYISKPFESRCPYCVDTKQVVFSGPKTQSDFGEWCLKSEHATFIAHNFKGFDGYFIIDYLYSLQLTPNIITMGGELKNGYFPHLFNKSENQQYVGPLPDVSFYSPNSMSTKKRSEFLFWYEKEVEKSVLFDFQKELLTYCVSDVDILRQCCLLFRGFFMEITKIPISKLESFGNIPTLPFNGYIMKVCKEMCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BN67</accession>
    <name>A0A9Q1BN67_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="18">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">HOLleu_29237</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="22">Nanhai2018</strain>
            <tissue evidence="22">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14 18">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 17">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="18">Membrane</location>
            <topology evidence="18">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 18">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="18"/>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BN67"/>
    <dbReference type="OrthoDB" id="377733at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140345">
        <property type="term" value="F:phosphatidylcholine flippase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02073">
        <property type="entry name" value="P-type_ATPase_APLT_Dnf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000025">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000188">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000014">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000001">
        <property type="entry name" value="Phospholipid-transporting ATPase IC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006539">
        <property type="entry name" value="P-type_ATPase_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032631">
        <property type="entry name" value="P-type_ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01652">
        <property type="entry name" value="ATPase-Plipid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF190">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16209">
        <property type="entry name" value="PhoLip_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 16 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="9 17 18" id="KW-0460">Magnesium</keyword>
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            <begin position="51"/>
            <end position="68"/>
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    <sequence length="1193" mass="135911" checksum="9691FA2DFB2A9EF1" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BQR5</accession>
    <name>A0A9Q1BQR5_HOLLE</name>
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        <submittedName>
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        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
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            <taxon>Metazoa</taxon>
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            <taxon>Eleutherozoa</taxon>
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        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="5">Muscle</tissue>
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        <text evidence="2">Belongs to the NAD(P)-dependent epimerase/dehydratase family. Dihydroflavonol-4-reductase subfamily.</text>
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        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366">
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    </dbReference>
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        <property type="entry name" value="STEROL-4-ALPHA-CARBOXYLATE 3-DEHYDROGENASE, DECARBOXYLATING"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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            <begin position="1"/>
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            <begin position="22"/>
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            <begin position="14"/>
            <end position="252"/>
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    </feature>
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    </evidence>
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    </evidence>
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            <dbReference type="Proteomes" id="UP001152320"/>
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    </evidence>
    <sequence length="383" mass="42072" checksum="EFD76AB6B7373392" modified="2023-09-13" version="1">MGLLFSSVLSSSGVLVTGASGYVASSTVNRLLADGYNVRGTVRSLRNTQKITPLTSLAHGKRGTLELVEADLLTPETWKSAVEGCSHVLHIASPFPVNQPKDADEVIRPAVEGTLNVLKACKEAGSVKRVTLTSSIASVGMYNTTRPLTEDDWTDPGRGGAEDSPYIISKVMAEKEAFKYVSGLADKDKFEICSLLPGFIVGPVITNQAATSLEFIKRIMVGGDPLVPLIGFSFVDIRDVVEAQIVSLTSPKVPGNRFIITDKFMQYHKICEMLNDEFRHQGYSPSSRLAPRFLIRLMSLFDEMAESMNVMWGIVTEFDNSKMRNVLGITPRDSRKAVIDMAYSLIDNGYIKKSKLYGQKANQIYTLDRTSPRPLYTGSVARE</sequence>
</entry>
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    <accession>A0A9Q1BR51</accession>
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        <submittedName>
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        </submittedName>
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        <dbReference type="NCBI Taxonomy" id="206669"/>
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            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8031337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BR51"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8031337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="59" mass="7309" checksum="E85A94FFE66B2843" modified="2023-09-13" version="1">MKYFIFNCKRQKLELDINSFFYKMKFALQVERFNYFKRSRVSKSKVSFPDLRKTFASCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BSA2</accession>
    <name>A0A9Q1BSA2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Endonuclease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HOLleu_24913</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Nanhai2018</strain>
            <tissue evidence="16">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BSA2"/>
    <dbReference type="OrthoDB" id="10051637at2759"/>
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        <property type="component" value="Chromosome 12"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="RNase_HI_RT_Ty3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06094">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 17.6-like Protein"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
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    </dbReference>
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        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
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        <property type="entry name" value="Peptidase_A2_cat"/>
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        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
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        <property type="entry name" value="RNaseH_sf"/>
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    <dbReference type="InterPro" id="IPR034132">
        <property type="entry name" value="RP_Saci-like"/>
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    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
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    <dbReference type="InterPro" id="IPR041577">
        <property type="entry name" value="RT_RNaseH_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17919">
        <property type="entry name" value="RT_RNaseH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ASP_PROT_RETROV"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0229">DNA integration</keyword>
    <keyword evidence="5" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="3" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="10" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Peptidase A2" evidence="13">
        <location>
            <begin position="23"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase" evidence="14">
        <location>
            <begin position="200"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase catalytic" evidence="15">
        <location>
            <begin position="708"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="974"/>
            <end position="1017"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ8031654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1035" mass="117078" checksum="AC89EA1BAE67388F" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BTP1</accession>
    <name>A0A9Q1BTP1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">E3 ubiquitin-protein ligase TRIM56</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_26208</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006513">
        <property type="term" value="P:protein monoubiquitination"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Bbox1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19756">
        <property type="entry name" value="Bbox2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047153">
        <property type="entry name" value="TRIM45/56/19-like"/>
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        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25462">
        <property type="entry name" value="BONUS, ISOFORM C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25462:SF229">
        <property type="entry name" value="TRANSCRIPTION INTERMEDIARY FACTOR 1-BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BBOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69322">
        <property type="entry name" value="Tricorn protease domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="B box-type" evidence="3">
        <location>
            <begin position="43"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="205"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8032636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="665" mass="75481" checksum="097FCB579EA397C5" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BTR9</accession>
    <name>A0A9Q1BTR9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">SH3 and multiple ankyrin repeat domains protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_26079</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Postsynaptic density</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BTR9"/>
    <dbReference type="OrthoDB" id="445896at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043197">
        <property type="term" value="C:dendritic spine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035255">
        <property type="term" value="F:ionotropic glutamate receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030160">
        <property type="term" value="F:synaptic receptor adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06746">
        <property type="entry name" value="PDZ_SHANK1_3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00174">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
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            <begin position="781"/>
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            <dbReference type="EMBL" id="KAJ8032528.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1253" mass="136888" checksum="5118FC5C5EDFA9F0" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BU94</accession>
    <name>A0A9Q1BU94_HOLLE</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ORF">HOLleu_26304</name>
    </gene>
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        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
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        </lineage>
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        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="KAJ8032704.1"/>
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        <property type="component" value="Chromosome 12"/>
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        </location>
    </feature>
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        </location>
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            <begin position="89"/>
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        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAJ8032704.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="105" mass="11173" checksum="715EE9AC85D81969" modified="2023-09-13" version="1">MLGGIKFSLKWTRLDSAPSPSPVAAHSGPIPIPEPPIPGQFRVQKKLDSSPELSPSPDSGVTALLVNTTNPSPIVLNLPLYFIEDSGSLKGNKQTNKQASKQTNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BUC3</accession>
    <name>A0A9Q1BUC3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Voltage-dependent calcium channel subunit alpha-2/delta-2</fullName>
        </submittedName>
    </protein>
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        <name evidence="15" type="ORF">HOLleu_22851</name>
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        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
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        <dbReference type="NCBI Taxonomy" id="206669"/>
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        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Nanhai2018</strain>
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        </source>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
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    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8032799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="term" value="C:voltage-gated calcium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Ca_channel_alpha-2/delta"/>
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        <property type="entry name" value="VWF_A"/>
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        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10166">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL SUBUNIT ALPHA-2/DELTA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10166:SF43">
        <property type="entry name" value="VWA N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22673">
        <property type="entry name" value="MCP-like_PDC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="VWA_2"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
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    <keyword evidence="4" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="3" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0407">Ion channel</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="8" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="domain" description="VWFA" evidence="14">
        <location>
            <begin position="276"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ8032799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1188" mass="134432" checksum="197A99ACB9B20B90" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BWT7</accession>
    <name>A0A9Q1BWT7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Tenascin-R</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_24402</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BWT7"/>
    <dbReference type="OrthoDB" id="7250310at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00087">
        <property type="entry name" value="FReD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19941">
        <property type="entry name" value="TIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050373">
        <property type="entry name" value="Fibrinogen_C-term_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036084">
        <property type="entry name" value="Ser_inhib-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025615">
        <property type="entry name" value="TILa_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040941">
        <property type="entry name" value="GGGWT_bact"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143">
        <property type="entry name" value="FIBRINOGEN/TENASCIN/ANGIOPOEITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12714">
        <property type="entry name" value="TILa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00186">
        <property type="entry name" value="FBG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57567">
        <property type="entry name" value="Serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="3">
        <location>
            <begin position="99"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="315"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="3">
        <location>
            <begin position="351"/>
            <end position="568"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8033996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="568" mass="63938" checksum="E88A1988B98E84FC" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BWU3</accession>
    <name>A0A9Q1BWU3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HYR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_20970</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BWU3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003410">
        <property type="entry name" value="HYR_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24273">
        <property type="entry name" value="FI04643P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24273:SF32">
        <property type="entry name" value="HYALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02494">
        <property type="entry name" value="HYR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50825">
        <property type="entry name" value="HYR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="HYR" evidence="2">
        <location>
            <begin position="28"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="HYR" evidence="2">
        <location>
            <begin position="117"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8034225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="235" mass="25312" checksum="61CBE57AEF569A53" modified="2023-09-13" version="1">MTVTCTCTDSCQDTDSCTFIVNVDGFLSENTPPTITCPPPVTVISLPGNIGNIATYNPPQCIDAEDNQNQLQTSCSKPSGAFFQGVGPMTVTCTCTDSYQDTDSCTFNIFKCLCFLSENTPPTIICPPPVTMISLPGNIGNIATYNPPQCFDAEDNQNQLQTSCSQPSGAFFQGVGPMTVTCTCTDSCQDTDSCTFIVNIDGEGQSLSLTLTQHVTLKNICSRRDVLRLEKVFKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BXW9</accession>
    <name>A0A9Q1BXW9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_21895</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BXW9"/>
    <dbReference type="OrthoDB" id="9995178at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912:SF35">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912">
        <property type="entry name" value="IP13529P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="113"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8034877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="885" mass="102145" checksum="ADE8E8825AE868BA" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BZ63</accession>
    <name>A0A9Q1BZ63_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Cell division cycle-associated 7-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_22406</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BZ63"/>
    <dbReference type="OrthoDB" id="298344at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040221">
        <property type="entry name" value="CDCA7/CDA7L"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018866">
        <property type="entry name" value="Znf-4CXXC_R1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31169">
        <property type="entry name" value="OS05G0300700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31169:SF8">
        <property type="entry name" value="ZINC-FINGER DOMAIN OF MONOAMINE-OXIDASE A REPRESSOR R1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10497">
        <property type="entry name" value="zf-4CXXC_R1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="12" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Zinc-finger" evidence="11">
        <location>
            <begin position="218"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="47"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="164"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="60"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="97"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="139"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="164"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF10497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8035244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="321" mass="36790" checksum="6A692AA104EB4697" modified="2023-09-13" version="1">MAAELFKNSNIFKDILKECSYEERRQKNIEENKAVLQKLLADLQGLPGVPLLTPAKKKSQQPQRSPKSRNSIDHAYTPRKNPSRRARPSHLPDTPVRRSRRLTIKKRQTEQQDVFPLSGEKLVIKFGPPRKRKLSGSDGESDTDSWEDGEIEVFEDEVQLDEEEEWLADSDEDSELVPTPTKKRRKSQIPLTYEHRSPEEITQEELNMVAKTVKDKIYNQEYGSTCHQCRQKTLDMKTICRSSSCHGVRGKFCGPCLRNRYGESAEEALLDADWTCPPCRGICNCSFCRKKAGRHATGILIHLAKERGFKSVREYLDSLAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BZW0</accession>
    <name>A0A9Q1BZW0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_19863</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BZW0"/>
    <dbReference type="OrthoDB" id="10064299at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040495147" evidence="2">
        <location>
            <begin position="21"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="288"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="351"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="305"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="375"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8036011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="433" mass="48613" checksum="4152E321D5D6FCBB" modified="2023-09-13" version="1">MLAKSVLLIVLLGLVHFTSTAPVKDADAEKETPIDLVNNDVDQQTDTDAKDDVSPTPEVLAKPEETDNVNKEVTNYQTIETDDHGKVEPVIADTEGDEDTGLTEEEAQELLLELSDPEEVAQFIVASDDAQGFLQALRLLTDQNYISLKLAYYLRDRVMEQLRRMLMIQEMELSRTLLNNYEPADFTPDQLPNDVPPAPYYDFNTFPATNDMYSEDVSPYEIYEQTLREENSRALAEIAEAVAQKIAKGDLTPESGVKIMNTVASLLPDSYWEDALNLFEEGEDNVYPLAYDEPTSDYSSASESLEGDVIDLTENSDETSTEDEDDTGDNLEAELKNKNRVLENALEKQLAEKAHSKETITPSEGEDEVHLQQDDSSDEFDLTDEDYQELLDWLQNELQDSTKQQEQKPGALLVKDDDDDGKVQGEQPEENSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C0J1</accession>
    <name>A0A9Q1C0J1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tyr recombinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_19640</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8035841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C0J1"/>
    <dbReference type="OrthoDB" id="6084584at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="entry name" value="Intergrase catalytic core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyr recombinase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8035841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="134" mass="15086" checksum="A79AA8A56C955E4D" modified="2023-09-13" version="1">MRKISEKAGCTQKYTNHSLRATTCTVLDEAGIPSRHIMSVTGHRSESSLKHYSCTSDQKKKLMSKELANQTSENVPEVQSNADAILDNLPEVLLTNSQEQPILNESNQFNIRNSMESSSIQQFHFHGPVTFYDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C171</accession>
    <name>A0A9Q1C171_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Carbohydrate sulfotransferase</fullName>
            <ecNumber evidence="9">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_20541</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Golgi apparatus membrane</location>
            <topology evidence="1 9">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the sulfotransferase 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="9"/>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C171"/>
    <dbReference type="OrthoDB" id="2019940at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016051">
        <property type="term" value="P:carbohydrate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018011">
        <property type="entry name" value="Carb_sulfotrans_8-10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005331">
        <property type="entry name" value="Sulfotransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12137">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12137:SF54">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03567">
        <property type="entry name" value="Sulfotransfer_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="8 9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="41"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="46"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8036535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="389" mass="45397" checksum="3B6D63EB48F65370" modified="2023-09-13" version="1">MNTKGTLGVLVFILCVICIFHIYQSSELSFSSRGNIIEEDGDEDFSSTSVLSSYDSSDPDQTNEKDRPENSLSGPYNEHFHEQQEEDFYQHLKDRISHLEAPNSLYYQHHLERQRQRHAQLASTCSTMKKQSFSLSDMSRNQKNIWVNDKYKFVYCATPKAACTSWKSVLLVLDGIATSTDQFSQKTINSKSGVWMKQLWRYSDEEQKDIMKTYTKFMFSRNPFSRVLSAFQNKLAPNSTFERALKWQVNLGRKIITRYRNRPQRNKPYDLKFSEFGRYISDPSVHSFNKHWSLQYLQCRPCDLDYTILGHFETLEEDSKFILKVIGAENVVSFPSSNHSSPTNSSNESIFTSNYNTLSFDELKNLYEVYRKDFEMFGYPVPERLVGKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C245</accession>
    <name>A0A9Q1C245_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Actin-binding protein IPP</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_17492</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8036848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C245"/>
    <dbReference type="OrthoDB" id="2359033at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051481">
        <property type="entry name" value="BTB-POZ/Galectin-3-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24410:SF48">
        <property type="entry name" value="BTB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24410">
        <property type="entry name" value="HL07962P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="1">
        <location>
            <begin position="77"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8036848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="522" mass="61024" checksum="B913FE7CA1C3014D" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C3Z8</accession>
    <name>A0A9Q1C3Z8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Sodium-independent sulfate anion transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_18910</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C3Z8"/>
    <dbReference type="OrthoDB" id="288203at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07042">
        <property type="entry name" value="STAS_SulP_like_sulfate_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="entry name" value="STAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011547">
        <property type="entry name" value="SLC26A/SulP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001902">
        <property type="entry name" value="SLC26A/SulP_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00815">
        <property type="entry name" value="sulP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11814">
        <property type="entry name" value="SULFATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01740">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00916">
        <property type="entry name" value="Sulfate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SpoIIaa-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="62"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="89"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="111"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="140"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="170"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="220"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="262"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="316"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="356"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="394"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="421"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="458"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="domain" description="STAS" evidence="6">
        <location>
            <begin position="486"/>
            <end position="596"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8037957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="621" mass="68537" checksum="80CE27B718C007C5" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C6E3</accession>
    <name>A0A9Q1C6E3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">1-acyl-sn-glycerol-3-phosphate acyltransferase</fullName>
            <ecNumber evidence="5">2.3.1.51</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_16245</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 1-acyl-sn-glycero-3-phosphate + an acyl-CoA = a 1,2-diacyl-sn-glycero-3-phosphate + CoA</text>
            <dbReference type="Rhea" id="RHEA:19709"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57970"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="EC" id="2.3.1.51"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 2/3.</text>
    </comment>
    <comment type="domain">
        <text evidence="5">The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.51" evidence="5"/>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C6E3"/>
    <dbReference type="OrthoDB" id="202234at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003841">
        <property type="term" value="F:1-acylglycerol-3-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006654">
        <property type="term" value="P:phosphatidic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07989">
        <property type="entry name" value="LPLAT_AGPAT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004552">
        <property type="entry name" value="AGP_acyltrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00530">
        <property type="entry name" value="AGP_acyltrn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10434">
        <property type="entry name" value="1-ACYL-SN-GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10434:SF11">
        <property type="entry name" value="1-ACYL-SN-GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="5" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="130"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="7">
        <location>
            <begin position="95"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8038736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="277" mass="31269" checksum="5D05CDBE8D51C3E7" modified="2023-09-13" version="1">MELEWAQIAIIALLLALIPLYEMNSSFKYYAKFALYYALLLAESVLVCILSLPRGRDPYNSRWASMVMPYTSLLLGVKFEVRGKENFRKVQNKPCVVVANHQSSLDLLGLTGTDVFTELTPIAGLAKKELMYAGTFGMCLLMIGTVFINRLQSEEAKETLERIKERMRETHLKLLVFPEGTRNQEAGLLPFKKGAFHLAISAQVPILPLVFSSYSDFYSKKEKRFTTGKVYVTALPPIPTEGKTLEDLPAFTESVRKQMLFTYNESSTPRLESNGLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C711</accession>
    <name>A0A9Q1C711_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Death domain-associated protein 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_16859</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C711"/>
    <dbReference type="OrthoDB" id="7492809at2759"/>
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        <property type="component" value="Chromosome 7"/>
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        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016605">
        <property type="term" value="C:PML body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050681">
        <property type="term" value="F:nuclear androgen receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.810">
        <property type="entry name" value="Daxx helical bundle domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046378">
        <property type="entry name" value="DAXX_histone-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046426">
        <property type="entry name" value="DAXX_histone-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038298">
        <property type="entry name" value="Daxx_N_sf"/>
    </dbReference>
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        <property type="entry name" value="DEATH DOMAIN-ASSOCIATED PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12766">
        <property type="entry name" value="DEATH DOMAIN-ASSOCIATED PROTEIN 6 DAXX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20920">
        <property type="entry name" value="DAXX_hist_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="8" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Daxx histone-binding" evidence="12">
        <location>
            <begin position="251"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="109"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="326"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="458"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="622"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="146"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="110"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="326"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="347"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="366"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="478"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="521"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="540"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="564"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="659"/>
            <end position="669"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF20920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8039214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="678" mass="75548" checksum="03B17C7530ED2379" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C7A4</accession>
    <name>A0A9Q1C7A4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_13780</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8039697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome 6"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8039697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="117" mass="12834" checksum="42E23EFDA39F228D" modified="2023-09-13" version="1">MPHIERKNPIVFGGGQRSPGVTGGQNLKTLLTQYLEMRYGKRKNPIIFGGGQRSPGVTGGQTLKTSLTRYLKMPHIERKNPIVFGGGQRSPGVTRGQNLKTLLTQYLEVGSLGEFHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-02-05" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C8L8</accession>
    <name>A0A9Q1C8L8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_14453</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10054042at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="RT_pepA17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040676">
        <property type="entry name" value="DUF5641"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008042">
        <property type="entry name" value="Retrotrans_Pao"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331:SF1">
        <property type="entry name" value="GAG-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18701">
        <property type="entry name" value="DUF5641"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05380">
        <property type="entry name" value="Peptidase_A17"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="3">
        <location>
            <begin position="1465"/>
            <end position="1654"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="52"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="22"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="38"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8040223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1770" mass="201052" checksum="3DF2BDAC2F95316F" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C904</accession>
    <name>A0A9Q1C904_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Tyrosine-protein kinase</fullName>
            <ecNumber evidence="9">2.7.10.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_14699</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6 9">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the protein kinase superfamily. Tyr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.2" evidence="9"/>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C904"/>
    <dbReference type="OrthoDB" id="535945at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004715">
        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000216">
        <property type="entry name" value="Tyrosine-protein kinase SYK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050198">
        <property type="entry name" value="Non-receptor_tyrosine_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24418">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 9 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="1 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 9" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="SH2" evidence="11">
        <location>
            <begin position="19"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="11">
        <location>
            <begin position="173"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="451"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="318"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="322"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="347"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="371"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="482"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00051245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8040418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="729" mass="82778" checksum="CD37B3753C4D7B0D" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CA68</accession>
    <name>A0A9Q1CA68_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Xyloglucan galactosyltransferase GT19</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_11958</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 47 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CA68"/>
    <dbReference type="OrthoDB" id="1924787at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015012">
        <property type="term" value="P:heparan sulfate proteoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004263">
        <property type="entry name" value="Exostosin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040911">
        <property type="entry name" value="Exostosin_GT47"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11062">
        <property type="entry name" value="EXOSTOSIN HEPARAN SULFATE GLYCOSYLTRANSFERASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11062:SF281">
        <property type="entry name" value="EXOSTOSIN-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03016">
        <property type="entry name" value="Exostosin_GT47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Exostosin GT47" evidence="3">
        <location>
            <begin position="95"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="53"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="69"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8041212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="471" mass="54229" checksum="F341E2568474AFB8" modified="2023-09-13" version="1">MAGNRLFTSSAQFEFLTSSAIQLSLSLCLFHFPGYTAKGKKADVTPELRKQLQAEIEDSSSHQSLPSNHETKEKERPKSAYQENHKNLETAEERPSLLVYVYDIPSMFTTDLLYCSRNHTDNIDQRSEKLLATQSLKGMLQNTTFGESNLEVYLHRALLQSSYRTHDATKADVLFIPFYASFACNCRTLHRINITNLHDQLWQFVDSALPYFRNGNPSRPHFMSLGKPERLYWTKNCPLLRHKVHTKDIIFIGVEKESNLGLRKYTGRDSSTMIVAPYPAHKPVVVKQETRNIFIFFYDSIHSSHEVTRILRQDISAVHYPSDVTEIDTFVARFVDYPEGRSVSQLACNWMRRSVFCLQAPVDTHIQAAFYEAIAAGCIPVTFLQKKTKNQVQFPFEDKIDFSGFTINISPGDILSRNQNVTWYLQSLPKLRIEIMQKKLKQYSTQLLYSSEGDPNNAFEMIIDEMRRTTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CBD4</accession>
    <name>A0A9Q1CBD4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosomal RNA-processing protein 42</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_12287</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RNase PH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CBD4"/>
    <dbReference type="OrthoDB" id="272245at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035925">
        <property type="term" value="F:mRNA 3'-UTR AU-rich region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000467">
        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071028">
        <property type="term" value="P:nuclear mRNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071035">
        <property type="term" value="P:nuclear polyadenylation-dependent rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034473">
        <property type="term" value="P:U1 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034476">
        <property type="term" value="P:U5 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11367">
        <property type="entry name" value="RNase_PH_RRP42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="entry name" value="GHMP Kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015847">
        <property type="entry name" value="ExoRNase_PH_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036345">
        <property type="entry name" value="ExoRNase_PH_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050590">
        <property type="entry name" value="Exosome_comp_Rrp42_subfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11097:SF8">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT RRP42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11097">
        <property type="entry name" value="EXOSOME COMPLEX EXONUCLEASE RIBOSOMAL RNA PROCESSING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03725">
        <property type="entry name" value="RNase_PH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55666">
        <property type="entry name" value="Ribonuclease PH domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="5" id="KW-0271">Exosome</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="7">
        <location>
            <begin position="32"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="8">
        <location>
            <begin position="197"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00042523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8041464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="289" mass="31033" checksum="384104FE14E0FC29" modified="2023-09-13" version="1">MASTQLSDGEKTFIIHGVLDNLRNDGRTCENYRQIEVECGVVSNTSGSSRLRLSNTEVLVGIKAEMGQPKPQSPKTGFLEFFVDFSANASPEFEGRGGNDLSEDMSTRFAEVFQTNNILDFESLCIIPGQCCWVLYIDIVLLECGGNLLDAMSLAVKAALANTRIPKVTVLRDSEGGEDIEVSDDPDGYHTLEISKVPLFVTLSKIGQKHVVDATLEEEACCSASLVVSVNSSGDFTGMVKKGSSSLSNDSIHDMLVTAKKVGIQLHKGLDTLLSEVGQSTAGRGFLVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CBE5</accession>
    <name>A0A9Q1CBE5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">T-box transcription factor TBX20</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_12613</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CBE5"/>
    <dbReference type="OrthoDB" id="7442607at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001708">
        <property type="term" value="P:cell fate specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd20193">
        <property type="entry name" value="T-box_TBX20-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.820:FF:000008">
        <property type="entry name" value="T-box transcription factor TBX20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.820">
        <property type="entry name" value="Transcription factor, T-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046360">
        <property type="entry name" value="T-box_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036960">
        <property type="entry name" value="T-box_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001699">
        <property type="entry name" value="TF_T-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018186">
        <property type="entry name" value="TF_T-box_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11267">
        <property type="entry name" value="T-BOX PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11267:SF190">
        <property type="entry name" value="T-BOX TRANSCRIPTION FACTOR TBX20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00907">
        <property type="entry name" value="T-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00937">
        <property type="entry name" value="TBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00425">
        <property type="entry name" value="TBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01283">
        <property type="entry name" value="TBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50252">
        <property type="entry name" value="TBOX_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="T-box" evidence="8">
        <location>
            <begin position="136"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="430"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8041721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="461" mass="52654" checksum="5BD07B5AF9983C1E" modified="2023-09-13" version="1">MESTDRMGKKDTLLRLSSRAAAFSIESLLKKKSTKRSNVEDSKHRTKHRSPYHPYRHRVHRDTEDKVDSYQHYHYHTSLNSEQYTNSISTSGNERNTVTTVCKPDTAVPFQSGRQCNECEGAGRCEAEMSKVQCHLEIKELWDRFHELGTEMIITKSGRRMFPALRVSFTGLDPKTRYTILLDIWPVDNKRYRYAYHRSSWLVAGKADPPLKNRLYLHPDSPYTGEHLSKQMISFEKVKLTNNALDSQGHIILNSMHRYQPRVHIVRASKVDPDNLEKYLSSCHTKTFAFPETSFTAVTAYQNQLITRLKIDSNPFAKGFRDSSRLTDFERERVENLLHDKTTLASYHQSTQKCYPFPGAAASTFPFKPYASTYIEGLHCTNPASSGALSMASFVVPIPRQPSALSRAAENYAFYGRNCNIFHASHRSTPPLYKNHKAPSHEQYSSVMVDGTSKPPSTKEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CC87</accession>
    <name>A0A9Q1CC87_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Polycomb group protein Pc</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_09488</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8042671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CC87"/>
    <dbReference type="OrthoDB" id="1918685at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035102">
        <property type="term" value="C:PRC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023779">
        <property type="entry name" value="Chromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052458">
        <property type="entry name" value="PcG_PRC1-like_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46389">
        <property type="entry name" value="POLYCOMB GROUP PROTEIN PC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46389:SF3">
        <property type="entry name" value="POLYCOMB GROUP PROTEIN PC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00598">
        <property type="entry name" value="CHROMO_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chromo" evidence="4">
        <location>
            <begin position="267"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="125"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="134"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="148"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="184"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="214"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="236"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8042671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="323" mass="35668" checksum="85805B7400312942" modified="2023-09-13" version="1">MPHHKGKINTNFVQNKKQRLKYKNRRIKTLLSKGMELCSMTNCSVFIYTKDELNHIRVAGSQDMLAAYHGAGIKSTMFSETESTSSEIVQGPTARPSAPTCSSNIMTCDAATACTTSSTASQQDKPAVALVCRRSHESEPHRSHGDNSPMPKTSPSATTTSDSSVCNVPPRPTSSTACVEPTQEEVQLQNASTLSASENNLPHTGPDTGRHSPLNHTYTQPTTQPTEVNSPRPADDSDEEDADEEAPLSLVKAVLKLQQDDPLAQVYQAEKITDSRIFLGSKQYFVKWKGWSDKHNTWEPEEHIIDKKLIALFLSQQNKEKEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CFM6</accession>
    <name>A0A9Q1CFM6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tryptophan--tRNA ligase, cytoplasmic</fullName>
            <ecNumber evidence="3">6.1.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Tryptophanyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_07232</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.2" evidence="3"/>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CFM6"/>
    <dbReference type="OrthoDB" id="10261385at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004830">
        <property type="term" value="F:tryptophan-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006436">
        <property type="term" value="P:tryptophanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00806">
        <property type="entry name" value="TrpRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000033">
        <property type="entry name" value="tryptophan--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.240.10">
        <property type="entry name" value="Tyrosyl-Transfer RNA Synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002305">
        <property type="entry name" value="aa-tRNA-synth_Ic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002306">
        <property type="entry name" value="Trp-tRNA-ligase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00233">
        <property type="entry name" value="trpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10055:SF1">
        <property type="entry name" value="TRYPTOPHAN--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10055">
        <property type="entry name" value="TRYPTOPHANYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00579">
        <property type="entry name" value="tRNA-synt_1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01039">
        <property type="entry name" value="TRNASYNTHTRP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 12 14" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="10"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ8044471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="373" mass="41997" checksum="3AB0996E154CDE95" modified="2023-09-13" version="1">MTDTEKLAAQVNSLTLEESTNPPATDDDDDDQIVDPWQVVSKSGKGVDYNKLIKKFGSTRIDDALLERFEKVTGHKPHHFLRRGIFFSQRDVHFILNCYEAKKPFFLYTGRGPSSEAMHLGHLIPFMFTKWLQEVFDVPLVIQMTDDEKFLWKDLAAEEALRLTYENAKDIIAQGFDVNKTFIFSDMEYIGTCPAFYKNMLKIQKCVTFNQVKGIFGFGDSDCIGKISFPATQAAPSFSNSFPHIFGENDAGKVPCFIPCAIDQDPYFRMTRDVAPRLGYPKPALIHSTFFPALQGAQTKMSASDPNSSIFLTDTQKQIKTKGIAEADETTPPSHDSFHQKIPTLPQPSCEHPLNIPVPFDLFETNSHEDGSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CFY4</accession>
    <name>A0A9Q1CFY4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Fucosyltransferase</fullName>
            <ecNumber evidence="11">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_11338</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Endomembrane system</location>
            <topology evidence="10">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Golgi apparatus</location>
            <location evidence="11">Golgi stack membrane</location>
            <topology evidence="11">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the glycosyltransferase 10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="11"/>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8044003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CFY4"/>
    <dbReference type="OrthoDB" id="427096at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046920">
        <property type="term" value="F:alpha-(1-&gt;3)-fucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11660:FF:000002">
        <property type="entry name" value="Alpha-(1,3)-fucosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11660">
        <property type="entry name" value="Glycosyl transferase family 10, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055270">
        <property type="entry name" value="Glyco_tran_10_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001503">
        <property type="entry name" value="Glyco_trans_10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038577">
        <property type="entry name" value="GT10-like_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11929">
        <property type="entry name" value="ALPHA- 1,3 -FUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11929:SF145">
        <property type="entry name" value="ALPHA-(1,3)-FUCOSYLTRANSFERASE FUT-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00852">
        <property type="entry name" value="Glyco_transf_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="Fucosyltransferase C-terminal" evidence="12">
        <location>
            <begin position="194"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00060399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8044003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="431" mass="50380" checksum="2F796B93EE74346F" modified="2023-09-13" version="1">MKVHVTKYFSKKTCFTVLLCFVLYVTLVVLGEGSNLSESRDILTFMHAASFGSLFPSISTARILNVVSYKKKVGWHFVHFPCMYYCRDQNVRVRVSLGTLPHHIDSADAVTFSTGPFTHEEWKDLHSIRPRDQVWILATQEPASVMSDLLPPKEFRYDTYNLSFTFHTLTNIDGAYGKYEPFPAEQESSRNIFQEKTEMAAWVSTRHCGGMNWDRTRFVKDLGKHVPIHMYGGCGNRTLRRGIYWSKGTLQKYKFHVSLENSCCSEYITEKFWNALEHFESIPIVIGGTKEEYTTLGPPHSFIHAEDFGSMEELGKYIMTVAKNETLYNSFHDWRKKGKVSQQQFRQRFPVFKDGGCKVARKILELEQSQKLFKFDAYDAHWWGSCYRCGSQSWIQDYIFWKTLTRNRQSWEDDRVLFWMEDEDVHKITSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CHV3</accession>
    <name>A0A9Q1CHV3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_08607</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CHV3"/>
    <dbReference type="OrthoDB" id="9973206at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8045573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="244" mass="27699" checksum="EAC20D1DBC2737D4" modified="2023-09-13" version="1">MMAFYVTLPSNSSMDVYPENTLSNYRVKLPTSLQLSGEWEVGLVEIAYPHSWYVLSPDSSKISIRSEQDSSFREVDLKGRHFRRIEGVASHLLPGYLRDLYSITYNDTTCKVTITPEPGHELLLEGMICSTLGFEDPCVITTTTEAPYPAALNAVHHLFVYTDIIESQVVGHVKAPLLKTVPVHGDFGDTSNIEYQNPHYYPLRQSVIDTIEIDIRDDTGKKVSFERGRVIVKLHFRQKRSSYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CIN3</accession>
    <name>A0A9Q1CIN3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_08256</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CIN3"/>
    <dbReference type="OrthoDB" id="10051448at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8045279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="228" mass="27210" checksum="F44DC5658AA4A23A" modified="2023-09-13" version="1">MKIRQKIFLGVKIRPAEQRDLDFEINAKYIPQDFHQRDINIDEGLHLKVLKEMKRLTGKRVAQMVLDFERAVWNAIPLVFPDVTIRGYAFHWSQCVWGKIQETGLTPAYINDEGTHKFCRIYHIYHTNTFSQYSTIWNLERKASTDQLRQLTDYIRDQWIEENLWNPAAWCVFKQPIRTNNDVEGWQRALNRHARRGNITLYLLVKLLREQAYLVNMQVGHVSFHKLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CIS5</accession>
    <name>A0A9Q1CIS5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_04828</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CIS5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8046217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="200" mass="23005" checksum="ECD36CF867F0F224" modified="2023-09-13" version="1">MDEESAEILNMFTTVRPRSDFPRIAMTFRIATTSHDLPLHTVRLFDVANGVFRHREESLPCRRSKVVQWAPGLSGQPAQVVNFALFGEGKATSLFLFRRKVYTVHNYRRIVVNRNTATVRQLGNTYIFPRYRASSKTSVRRCSYGGYSRECCAGWSRNRDGSCSRKFRRYPLSFLVNRSQGCRDCSLLLCSNCCPLSLRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CJK9</accession>
    <name>A0A9Q1CJK9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_09287</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8046105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CJK9"/>
    <dbReference type="OrthoDB" id="8946688at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21301:SF12">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21301">
        <property type="entry name" value="REVERSE TRANSCRIPTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8046105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="166" mass="19022" checksum="F96A3D79567431E9" modified="2023-09-13" version="1">MLHEGDIDEKTAEYLVPKDCETSRYYTLPKTHKAPDTSGHLKIRPVISGIGSPIERLSEFLDFFINPEVQKLDSFIKDSKDMVKVIEGVNEKGPLPPSTALFTIDVKAMYPSLPQYLSIKGVRKSLDSRQLKKPSTDYLLECLKLCRTENHFEFNGRFLLRYTVPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CJX2</accession>
    <name>A0A9Q1CJX2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">TNF receptor-associated factor 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HOLleu_09243</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Nanhai2018</strain>
            <tissue evidence="15">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8046070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CJX2"/>
    <dbReference type="OrthoDB" id="5947827at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005164">
        <property type="term" value="F:tumor necrosis factor receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043122">
        <property type="term" value="P:regulation of canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.210.10:FF:000001">
        <property type="entry name" value="TNF receptor-associated factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.210.10">
        <property type="entry name" value="Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002083">
        <property type="entry name" value="MATH/TRAF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012227">
        <property type="entry name" value="TNF_rcpt-assoc_TRAF_met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008974">
        <property type="entry name" value="TRAF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049342">
        <property type="entry name" value="TRAF1-6_MATH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049440">
        <property type="entry name" value="TRAF3/5_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001293">
        <property type="entry name" value="Znf_TRAF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10131:SF153">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10131">
        <property type="entry name" value="TNF RECEPTOR ASSOCIATED FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21355">
        <property type="entry name" value="TRAF-mep_MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21363">
        <property type="entry name" value="TRAF3_RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02176">
        <property type="entry name" value="zf-TRAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015614">
        <property type="entry name" value="TRAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00061">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57953">
        <property type="entry name" value="Trimerization domain of TRAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50144">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50145">
        <property type="entry name" value="ZF_TRAF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="8 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="12">
        <location>
            <begin position="55"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="TRAF-type" evidence="14">
        <location>
            <begin position="144"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="TRAF-type" evidence="14">
        <location>
            <begin position="198"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="MATH" evidence="13">
        <location>
            <begin position="423"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="TRAF-type" evidence="11">
        <location>
            <begin position="144"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="TRAF-type" evidence="11">
        <location>
            <begin position="198"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ8046070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="576" mass="65756" checksum="4934491AD86D73BD" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CKX3</accession>
    <name>A0A9Q1CKX3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ATP-binding cassette sub-family C member 9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_04979</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CKX3"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042626">
        <property type="term" value="F:ATPase-coupled transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF461">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C MEMBER SUR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="3">
        <location>
            <begin position="8"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8046339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="87" mass="9711" checksum="10BF9C4FA5021E70" modified="2023-09-13" version="1">MPAGEWTEIGERGINLSGGQKQRLNVARAMYASKDIVMLDDPLSALDVHVGELLFQEGIIKFLGNSRRTTILVTHHLQYLLKADYVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CM20</accession>
    <name>A0A9Q1CM20_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Kazal-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_05874</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CM20"/>
    <dbReference type="OrthoDB" id="88467at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005518">
        <property type="term" value="F:collagen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050840">
        <property type="term" value="F:extracellular matrix binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00104">
        <property type="entry name" value="KAZAL_FS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036058">
        <property type="entry name" value="Kazal_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13866:SF29">
        <property type="entry name" value="FOLLISTATIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13866">
        <property type="entry name" value="SPARC OSTEONECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07648">
        <property type="entry name" value="Kazal_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00280">
        <property type="entry name" value="KAZAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100895">
        <property type="entry name" value="Kazal-type serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51465">
        <property type="entry name" value="KAZAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="Kazal-like" evidence="6">
        <location>
            <begin position="109"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="166"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="180"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="200"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="229"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="280"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8047004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="299" mass="32348" checksum="A1F86C6D59418EC0" modified="2023-09-13" version="1">MFPQVGCVSPCTEDTPHRLFPRVGLHLYAETSSGINGHKMRVFIIIATVLILASLCVEAKKKKDKGAKPATNCHKFQKQCDKGKAAACRKLQRLCSENQQVNQQVDPRPAGISDCPTSCKGAERAKVCGTNGQTYYSECLLRKERCRTQSDLEVAYLGKCSRYVRPASQTAGKGGGDGSSSEEDDSEEGEDGQGMQVGQENEEIPGEPEVPEGPEESVMQGGQITPNDPEQEPEQESQDPEMESQNPEGDAENPEGESQLPEGESQNPDGESQVDAPTGDGNSESDPGRTNIQEFPEFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CM66</accession>
    <name>A0A9Q1CM66_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_06194</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CM66"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8047240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="69" mass="7980" checksum="8516473F39B4DA4B" modified="2023-09-13" version="1">MESSREFNFNAAFLELSRKRDCYHPSSPSLVVIRSWNQFLRYSTLQVYYCTIVNVHALPALGPDQANIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CMA5</accession>
    <name>A0A9Q1CMA5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein Wnt</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_06052</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Ligand for members of the frizzled family of seven transmembrane receptors.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Secreted</location>
            <location evidence="1 10">Extracellular space</location>
            <location evidence="1 10">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the Wnt family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CMA5"/>
    <dbReference type="OrthoDB" id="5945655at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005109">
        <property type="term" value="F:frizzled binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045165">
        <property type="term" value="P:cell fate commitment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19337">
        <property type="entry name" value="Wnt_Wnt5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2460.20:FF:000001">
        <property type="entry name" value="Wnt homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2460.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005817">
        <property type="entry name" value="Wnt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043158">
        <property type="entry name" value="Wnt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018161">
        <property type="entry name" value="Wnt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12027:SF77">
        <property type="entry name" value="PROTEIN WNT-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12027">
        <property type="entry name" value="WNT RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00110">
        <property type="entry name" value="wnt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01349">
        <property type="entry name" value="WNTPROTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00097">
        <property type="entry name" value="WNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00246">
        <property type="entry name" value="WNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 10" id="KW-0879">Wnt signaling pathway</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8047139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="421" mass="47617" checksum="0129D320017A257D" modified="2023-09-13" version="1">MEESWLCTNNTVAANSRIFEKPSVRISKTCESTEPHRRMKLRCNPVDNRFGVYGVSKLPNVVLWLLAAFVTITLTEAQSMWWDLALDSRIQQFNSFRNPELFILGSQPLCTELPGLSASQQKLCQLYQDHMGPIGEGAKLAIDECKFQFQQRRWNCSGLEGQGVFGRATTIASRETAFTYAVSSAAVAHSISRACRQGLLSTCLCSNVPRPADLDKQWEWGGCGDNIDYGIRFAREFVDAIEMESNPPRRSRAYALMKMNLHNNEAGRKTVFHSMGIQCKCHGVSGSCSLKTCWPEMLSFRDIGSMLREKYDGASPTLVNKKGKLINKDMRFNKPTRTDLVYLDESPDYCTFNPRVGSLGTQGRECNRTSMGTDGCDLMCCGRGYNSYTELVEERCKCKFKWCCYVRCKKCRRQVEKSVCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CU21</accession>
    <name>A0A9Q1CU21_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Immunoglobulin V-set domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_03843</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CU21"/>
    <dbReference type="OrthoDB" id="6353782at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8050584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="162" mass="18471" checksum="06421C4571141109" modified="2023-09-13" version="1">MKLFANVETFCFKTFKKMHLLESVTTALLPLVLLLPLMQFRGTLAAVRCNSPQYLDFGKVGTITCLFHEDFYAVLWYTEADRISNIPTLRYRNEVKAGPGYESGEFDVHSNGSLIISNVSLDHNEMFTVAYIRFKEERYKSIHVSVIVVGKSNKFVLVVSSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1GY02</accession>
    <name>A0A9Q1GY02_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_29866</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8027809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1GY02"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="199"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="219"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="253"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8027809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="303" mass="33224" checksum="350F5B9985FFAD88" modified="2023-09-13" version="1">MPRRSDRKNDHVSLKGNAGRVAISNKNSGQTRERSRTEGDASGVRLDHLSKVLSRSPDSVVLKKQDVKLELNLLGSETNSTADVISPSSSSPKRALHSHEREEDQSNTSGDTTPGVLKPEIGVERGRAQTSGSGDTGQLRSEKQYVDKTAVDSLKAHNQRKFVIRNAIWDEDGDISPNGPVLLRGSHQKLRLCRSLDPNILSSKSDNQQEEVNNPQQEKNSRNRTEKPGSLKARSRIRKGKLAGSPGSSNRLMPRKLWKGKATKLAASPPYDTSQPHPAWSPLGKQCGPPPTLTCLIWNEGKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H1G5</accession>
    <name>A0A9Q1H1G5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Bis(5'-nucleosyl)-tetraphosphatase [asymmetrical]</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Diadenosine 5',5'''-P1,P4-tetraphosphate asymmetrical hydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_28457</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Nudix hydrolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H1G5"/>
    <dbReference type="OrthoDB" id="276276at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004081">
        <property type="term" value="F:bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006167">
        <property type="term" value="P:AMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006754">
        <property type="term" value="P:ATP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03428">
        <property type="entry name" value="NUDIX_Ap4A_Nudt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051325">
        <property type="entry name" value="Nudix_hydrolase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003565">
        <property type="entry name" value="Tetra_PHTase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21340:SF0">
        <property type="entry name" value="BIS(5'-NUCLEOSYL)-TETRAPHOSPHATASE [ASYMMETRICAL]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21340">
        <property type="entry name" value="DIADENOSINE 5,5-P1,P4-TETRAPHOSPHATE PYROPHOSPHOHYDROLASE MUTT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01405">
        <property type="entry name" value="TETRPHPHTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="6">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00032644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8029130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="113" mass="13104" checksum="05AD949A23137E99" modified="2023-09-13" version="1">MESTIGHLPKGHVDPGEDFKAAALRETEEEAGLDETKFNIHNFEFRLEYSVKGRPKEVVYWLAELKDPDTPIRLSEEHQDYKWLPLSEACTYAKYEDLQKALKDAAEYIENSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H1S7</accession>
    <name>A0A9Q1H1S7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Zinc finger BED domain-containing protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_28161</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8028906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H1S7"/>
    <dbReference type="OrthoDB" id="10057873at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47501:SF5">
        <property type="entry name" value="HAT C-TERMINAL DIMERISATION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47501">
        <property type="entry name" value="TRANSPOSASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140996">
        <property type="entry name" value="Hermes dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="429"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="436"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8028906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="543" mass="60975" checksum="512377030648D17F" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H220</accession>
    <name>A0A9Q1H220_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_27078</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H220"/>
    <dbReference type="OrthoDB" id="10685640at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071222">
        <property type="term" value="P:cellular response to lipopolysaccharide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051713">
        <property type="entry name" value="T-cell_Activation_Regulation"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25466:SF9">
        <property type="entry name" value="FIBRONECTIN TYPE-III DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25466">
        <property type="entry name" value="T-LYMPHOCYTE ACTIVATION ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="47"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="504"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="385"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8030614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="563" mass="63899" checksum="36795D0E17B36195" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H2L2</accession>
    <name>A0A9Q1H2L2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_29378</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H2L2"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8029866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="59" mass="6738" checksum="64060A700A3377F3" modified="2023-09-13" version="1">MESSRKFNFNAAFLELSRTLALSLFAREPVPTLQHATGLLPYHRQRACTTSPWTKPGKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H443</accession>
    <name>A0A9Q1H443_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ABC transporter G family member 20</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_21610</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H443"/>
    <dbReference type="OrthoDB" id="10255969at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03230">
        <property type="entry name" value="ABC_DR_subfamily_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1710.10">
        <property type="entry name" value="abc type-2 transporter like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047817">
        <property type="entry name" value="ABC2_TM_bact-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000412">
        <property type="entry name" value="ABC_2_transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43038:SF3">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 20 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43038">
        <property type="entry name" value="ATP-BINDING CASSETTE, SUB-FAMILY H, MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12698">
        <property type="entry name" value="ABC2_membrane_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00164">
        <property type="entry name" value="ABC2TRNSPORT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51012">
        <property type="entry name" value="ABC_TM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="521"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="565"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="598"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="631"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="690"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="19"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-2" evidence="9">
        <location>
            <begin position="486"/>
            <end position="715"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8034677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="716" mass="79797" checksum="92ECFA80F15CBC3B" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H4U6</accession>
    <name>A0A9Q1H4U6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_23532</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H4U6"/>
    <dbReference type="OrthoDB" id="10519143at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45695:SF9">
        <property type="entry name" value="LEUCOKININ RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45695">
        <property type="entry name" value="LEUCOKININ RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="38"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="67"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="148"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="184"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8033329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="251" mass="29253" checksum="836EE81B0BD888AC" modified="2023-09-13" version="1">MEKLFTIAAIIMAIIVIMDGSIVYLIVKERLWRDDNAVLILNLMIGFMIHCVWQMSSLTCDTFQWPFPLPLILVLEIIAHVAILLTLVFLSSDRYYARKKKTLVKIYCREYAGKRVWRIHHKDYVTAVSNGTVNDRSWQKTHIESRRLTLTVAALVFGQCTTWVPIYVYMIVARKDIFDDPQNPIFVLLLFANFLAAPILIYLFSKQFRLYFKRHFLCCLYPDSARQQSWIDLSMESSRQSSLQGRSSPIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H5H5</accession>
    <name>A0A9Q1H5H5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">L-cysteine desulfhydrase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_23354</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H5H5"/>
    <dbReference type="OrthoDB" id="5978656at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43092:SF4">
        <property type="entry name" value="AMINOTRANSFERASE CLASS V DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43092">
        <property type="entry name" value="L-CYSTEINE DESULFHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotransferase class V" evidence="2">
        <location>
            <begin position="24"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8033195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="365" mass="40452" checksum="71D806D9229E0A92" modified="2023-09-13" version="1">MQKREADPDGWYRFGNEKSLYLKSVKQLADFVGASPDNLVLVENASTGTNTALRSIQLAAGDKVLVTSYTFDAVLMNVKVLLDEIPGLEKVQLELTFPIQSEQDIILQYETVISANPGIKVAILDHLTSQSAIRMPLKPLIDLCHSKGILALVDGAHAPGHLHLNLEELGADFYVGNIHKWLFAPRGCALMWVHPRHHDNTNPLVVSHNHMQKTLVDRFVPSATRDNSAYLVVPAALEFHHALGGLGVIQVRNSDLATAASKMLSEAWGSEEYPIPLDLRAPFMAMVAMPQVFQDHFPPDEDSCNKLMKRMHEKGIFLCVTNVDGKLWARLSVHVWNSMEDFIVVRDTVLEMAKEVPSVVNGGLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H5M1</accession>
    <name>A0A9Q1H5M1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Testis-expressed protein 49</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_20308</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H5M1"/>
    <dbReference type="OrthoDB" id="7085216at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038775">
        <property type="entry name" value="SPMIP11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35263">
        <property type="entry name" value="TESTIS-EXPRESSED PROTEIN 49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35263:SF1">
        <property type="entry name" value="TESTIS-EXPRESSED PROTEIN 49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22593">
        <property type="entry name" value="SPMIP11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8036357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="151" mass="17757" checksum="E848844C1750620D" modified="2023-09-13" version="1">MAFFNLTSLGYQDPIRSQILEPNPVYSYTFVHKIPEGSYVSFRPPTSKLPPINMNKYNQVIQPGSYDEHGGSYVKYTERLRKHTRSSKDPNELCKNSLTTSQSHGWWHTKDNPDLRQKEPWTYVPRHVHVSSEMTRFVKEMSQTNREFSLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H610</accession>
    <name>A0A9Q1H610_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein-lysine N-methyltransferase HOLleu_24079</fullName>
            <ecNumber evidence="5">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_24079</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">S-adenosyl-L-methionine-dependent protein-lysine N-methyltransferase that methylates elongation factor 1-alpha.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the class I-like SAM-binding methyltransferase superfamily. EFM5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="5"/>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H610"/>
    <dbReference type="OrthoDB" id="206354at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016279">
        <property type="term" value="F:protein-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03187">
        <property type="entry name" value="Methyltr_EFM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019369">
        <property type="entry name" value="Efm5/EEF1AKMT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041370">
        <property type="entry name" value="Mlase_EEF1AKMT1/ZCCHC4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13200">
        <property type="entry name" value="EEF1A LYSINE METHYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13200:SF0">
        <property type="entry name" value="EEF1A LYSINE METHYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10237">
        <property type="entry name" value="N6-adenineMlase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8033735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="216" mass="24327" checksum="864FDBCED40B0E24" modified="2023-09-13" version="1">MSSDSDEDDVPQLSAHALAALQEFYAEQTAIANEDEEEVAAKVGGIQEDWQLSQFWYDDDTASVLANEAITAAGVNGRIACISTPTLFQKLRQINSSCSAIIFEYDKRFEVYGDHFVFYDFNKPLELPEHIKAHSFDIVIGDPPFLTDDCLEPFSQTMKLLAKDKMILCTGAVMEASVKKLLDLEICNFQPKHAKNLANEFRCFANYTTTYLNNDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H6C0</accession>
    <name>A0A9Q1H6C0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Ammonium transporter Rh type C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_21816</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ammonium transporter (TC 2.A.49) family. Rh subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H6C0"/>
    <dbReference type="OrthoDB" id="534912at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008519">
        <property type="term" value="F:ammonium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097272">
        <property type="term" value="P:ammonium homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3430.10:FF:000012">
        <property type="entry name" value="Rh type C glycoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3430.10">
        <property type="entry name" value="Ammonium transporter AmtB like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029020">
        <property type="entry name" value="Ammonium/urea_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024041">
        <property type="entry name" value="NH4_transpt_AmtB-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002229">
        <property type="entry name" value="RhesusRHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11730">
        <property type="entry name" value="AMMONIUM TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11730:SF60">
        <property type="entry name" value="RH50, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00909">
        <property type="entry name" value="Ammonium_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00342">
        <property type="entry name" value="RHESUSRHD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111352">
        <property type="entry name" value="Ammonium transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="50"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="140"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="173"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="210"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="242"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="293"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="334"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="382"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="domain" description="Ammonium transporter AmtB-like" evidence="7">
        <location>
            <begin position="19"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8034814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="475" mass="50692" checksum="5E273F90931D37E9" modified="2023-09-13" version="1">MAWFRGKLSVVIIISQIIFILLFGLFGSYSDEATPTGKNDMGHANAINRFYGVFQDVHVMIFIGFGFLMTFLKKYGYGSVGFNFLLAAYAVQWSTIVRGLISGLNGGKFSISIENLIEADFSAAVVLISFGAILGKSSPLQLIIMTIIEVVLSVANEYIGLSLLKVTDVGGSMFIHAFGAYFGLGVAWVLQRKANEDNAKEGSTKTSDLFSMIGTVFLWMFWPSFNGALVEEDSRHRAVINTYFSLAACCITTFAISALSNKDGKVEMVAIQNATLAGGVAVGTSADLMINPWGAITVGAIAAIISTLGFKFCTPFLCEKLKLHDTCGVHNLHGLPGVLGGVIGAIAATFATEDLYGSSLGDIFSELSGTAPPRTAGSQAGYQILALCITLGISFVGGLVTGLLLKLNIWDEPQDADLFDDYPFWDDVSTGPIEDGVDVRYKRIPGQEVEGGNENANEDVNMTELTKGAAAEAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H6U1</accession>
    <name>A0A9Q1H6U1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_22509</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H6U1"/>
    <dbReference type="OrthoDB" id="10261408at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="5"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8035324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="90" mass="10210" checksum="D964680695CFA3BD" modified="2023-09-13" version="1">MSHLCHICQLPFTRGCNLRRHIERVHKHRHGDLEMYGGDSKKGEGKRQHDSSDEGSYDDISQSDTNDSASEPLEEDDMTDSSETDDSVVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H6V6</accession>
    <name>A0A9Q1H6V6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Sushi domain-containing protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_22570</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H6V6"/>
    <dbReference type="OrthoDB" id="6051552at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005533">
        <property type="entry name" value="AMOP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056619">
        <property type="entry name" value="C8-3_MUC4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051495">
        <property type="entry name" value="Epithelial_Barrier/Signaling"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003886">
        <property type="entry name" value="NIDO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001846">
        <property type="entry name" value="VWF_type-D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13802">
        <property type="entry name" value="MUCIN 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13802:SF59">
        <property type="entry name" value="SUSHI DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03782">
        <property type="entry name" value="AMOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23263">
        <property type="entry name" value="C8-3_MUC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06119">
        <property type="entry name" value="NIDO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00094">
        <property type="entry name" value="VWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00723">
        <property type="entry name" value="AMOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00539">
        <property type="entry name" value="NIDO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00216">
        <property type="entry name" value="VWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50856">
        <property type="entry name" value="AMOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51220">
        <property type="entry name" value="NIDO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51233">
        <property type="entry name" value="VWFD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0768">Sushi</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040184205" evidence="9">
        <location>
            <begin position="18"/>
            <end position="1276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1096"/>
            <end position="1122"/>
        </location>
    </feature>
    <feature type="domain" description="NIDO" evidence="12">
        <location>
            <begin position="107"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="AMOP" evidence="10">
        <location>
            <begin position="501"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="domain" description="VWFD" evidence="13">
        <location>
            <begin position="660"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="11">
        <location>
            <begin position="1034"/>
            <end position="1090"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1173"/>
            <end position="1276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1259"/>
            <end position="1276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ8035369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1276" mass="138824" checksum="BAF40DD5BB999B2C" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H6W2</accession>
    <name>A0A9Q1H6W2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Partner and localizer of BRCA2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_18683</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H6W2"/>
    <dbReference type="OrthoDB" id="10651282at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="181"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="14"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="189"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8037782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="587" mass="66584" checksum="F50E93E11D6919EB" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H726</accession>
    <name>A0A9Q1H726_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">GTP-binding protein Rhes</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_18986</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8038022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H726"/>
    <dbReference type="OrthoDB" id="265044at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031681">
        <property type="term" value="F:G-protein beta-subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052236">
        <property type="entry name" value="Small_GTPase_RasD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46149">
        <property type="entry name" value="MIP08469P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46149:SF3">
        <property type="entry name" value="MIP08469P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8038022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="205" mass="22970" checksum="646184052749D514" modified="2023-09-13" version="1">MASSAIGAKGVGIASAYLRDVFLLVYSIDSRESFQEALRLRQLIIETKRTARIKSVPMVIVGNKCDCANQREVDLEKAKRMISVGKRCSFIEVSAKENIQIVEMFRILFEHARLPNEMTPAMHKSMSGSTSPNTSPKMAQKGIVLRKRISDACGVISPNARRPSLRSDLLQVRDNALRGRSKKDDEDDDVFEDSCIHKKRGCEIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H7P8</accession>
    <name>A0A9Q1H7P8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Dynein heavy chain 6, axonemal</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HOLleu_20051</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Nanhai2018</strain>
            <tissue evidence="17">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynein heavy chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="5593012at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008569">
        <property type="term" value="F:minus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.2620:FF:000001">
        <property type="entry name" value="Cytoplasmic dynein heavy chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.1220:FF:000001">
        <property type="entry name" value="Dynein axonemal heavy chain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.710:FF:000004">
        <property type="entry name" value="Dynein axonemal heavy chain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.720:FF:000007">
        <property type="entry name" value="Dynein axonemal heavy chain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.100:FF:000004">
        <property type="entry name" value="Dynein axonemal heavy chain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.490.20:FF:000005">
        <property type="entry name" value="Dynein axonemal heavy chain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.180.20:FF:000004">
        <property type="entry name" value="Dynein axonemal heavy chain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001143">
        <property type="entry name" value="Dynein axonemal heavy chain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002141">
        <property type="entry name" value="Dynein heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1120:FF:000001">
        <property type="entry name" value="dynein heavy chain 2, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000223">
        <property type="entry name" value="Dynein heavy chain 3, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000063">
        <property type="entry name" value="dynein heavy chain 6, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.130:FF:000015">
        <property type="entry name" value="Dynein heavy chain 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.30:FF:000005">
        <property type="entry name" value="Dynein, axonemal, heavy chain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.280:FF:000009">
        <property type="entry name" value="Dynein, axonemal, heavy chain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.20:FF:000006">
        <property type="entry name" value="Dynein, axonemal, heavy chain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000362">
        <property type="entry name" value="Dynein, axonemal, heavy chain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.1220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.100">
        <property type="entry name" value="Dynein heavy chain, N-terminal domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.180.20">
        <property type="entry name" value="Dynein heavy chain, N-terminal domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.720">
        <property type="entry name" value="Region D6 of dynein motor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035699">
        <property type="entry name" value="AAA_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035706">
        <property type="entry name" value="AAA_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041658">
        <property type="entry name" value="AAA_lid_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042219">
        <property type="entry name" value="AAA_lid_11_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041589">
        <property type="entry name" value="DNAH3_AAA_lid_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042222">
        <property type="entry name" value="Dynein_2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043157">
        <property type="entry name" value="Dynein_AAA1S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041466">
        <property type="entry name" value="Dynein_AAA5_ext"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041228">
        <property type="entry name" value="Dynein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043160">
        <property type="entry name" value="Dynein_C_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024743">
        <property type="entry name" value="Dynein_HC_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024317">
        <property type="entry name" value="Dynein_heavy_chain_D4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004273">
        <property type="entry name" value="Dynein_heavy_D6_P-loop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013602">
        <property type="entry name" value="Dynein_heavy_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042228">
        <property type="entry name" value="Dynein_linker_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22878:SF68">
        <property type="entry name" value="DYNEIN HEAVY CHAIN 6, AXONEMAL-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22878">
        <property type="entry name" value="DYNEIN HEAVY CHAIN 6, AXONEMAL-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12774">
        <property type="entry name" value="AAA_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12775">
        <property type="entry name" value="AAA_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12780">
        <property type="entry name" value="AAA_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12781">
        <property type="entry name" value="AAA_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17857">
        <property type="entry name" value="AAA_lid_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18198">
        <property type="entry name" value="AAA_lid_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08393">
        <property type="entry name" value="DHC_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17852">
        <property type="entry name" value="Dynein_AAA_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18199">
        <property type="entry name" value="Dynein_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03028">
        <property type="entry name" value="Dynein_heavy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12777">
        <property type="entry name" value="MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-0969">Cilium</keyword>
    <keyword evidence="9 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0243">Dynein</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="11" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="16">
        <location>
            <begin position="1503"/>
            <end position="1606"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="16">
        <location>
            <begin position="2127"/>
            <end position="2296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="51"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="103"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="497"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1270"/>
            <end position="1289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="3494"/>
            <end position="3532"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="2944"/>
            <end position="3003"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="140"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="154"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="168"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="3512"/>
            <end position="3532"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ8036164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="4254" mass="483148" checksum="87E20736D20C2530" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H7W8</accession>
    <name>A0A9Q1H7W8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">DnaJ-like subfamily C member 9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_20293</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H7W8"/>
    <dbReference type="OrthoDB" id="110024at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:heat shock protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DnaJ homolog subfamily C member 9"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052594">
        <property type="entry name" value="J_domain-containing_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44144">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44144:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 9"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="HTH_DNAJC9"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="4">
        <location>
            <begin position="16"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="188"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="112"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="204"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8036344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="259" mass="29849" checksum="CF8C55CE95BC65EA" modified="2023-09-13" version="1">MSSFLDSCEKFFGSKNLYEVLNIAKDAPASKVKKAYYKLSLSVHPDRVPVNEKKSATAKFQVLGKVYSVLSDKDKRTLYDQTGIIDDEGSLNEEKDWYDFWRLLFKLSEEDIKCFEKNYRESGEELKDLKEAYLEFEGDMDEIMENVLCCTAEDEERFVETLQELINAGELPDFDAFSKESKKKKAARKRKAKKEEKEAEEMREELYKNAPVKDDSENALAALIQSRQGQRMDSLMASLEAKYCQPKKTKKGGKKASKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H7X3</accession>
    <name>A0A9Q1H7X3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_19789</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8035953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H7X3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040448816" evidence="2">
        <location>
            <begin position="21"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8035953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="178" mass="19423" checksum="A8CDBBE67DD7E1EA" modified="2023-09-13" version="1">MVIAYMVLSIMAAVMSGACCISTSSVTPYLRSKSANQGYPDYYWHSYGWRYDYDRGNKYATAHFAIYIVLDVIFALQMLMSILGASFTCGALYSRSNQQQAVFQISPQVPNQPFGPAPPYHAVATQGQPLTVPFNSLQQPLFHQTGMIGTTANPTNMAETTVFGTNTSYVGATATNKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H833</accession>
    <name>A0A9Q1H833_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Crk-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_20565</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H833"/>
    <dbReference type="OrthoDB" id="67310at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030971">
        <property type="term" value="F:receptor tyrosine kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035591">
        <property type="term" value="F:signaling adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007167">
        <property type="term" value="P:enzyme-linked receptor protein signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00173">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051184">
        <property type="entry name" value="Tyrosine-phos_adapter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19969:SF5">
        <property type="entry name" value="CRK-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19969">
        <property type="entry name" value="SH2-SH3 ADAPTOR PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="1 4" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH2" evidence="6">
        <location>
            <begin position="33"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="7">
        <location>
            <begin position="164"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="248"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="284"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="419"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="289"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="422"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8036554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="473" mass="53720" checksum="D1CB7666BBEA395D" modified="2023-09-13" version="1">MSARGHPRQSQIAPALPSRCTLTHQRGRRIPRWFHGLLDRRVAEDLLLKSPAKPKEGLFLVRQSSNRGGCFVISVCTGYQVNHYLIETLESMFYVKRESGGERGRDIQACDLVNLVECYKRLPLDISGLTLHQPLHRMTPLAFTTSLSPTPSSKDNHNYIPLYVDHRYYIGKEDFCAIDLETTSFNEGEPVTILLKENKKWWFGYNSKGDLGYVPSGLLSPSYDVEPRDGPFSIQGEEEEEERVEISEFAKTDDDEMDGTTQSDPNIGSKMSLDMNSNVVKSRFHMSQKSHEHVQRTDVDDSRELQRVPDSSSVSCSTKETKGASEIYVSSDYISACVQSHEQKLVNELLCAQRGISELSVDQGDREWTFEEMDVALLEEQIVPDMDMDTDFRPQGYLSEAFEKDLWTEHNQSWNLPIASSRVRENSENGTSHESEIESQESLETDAPGSEAYDPVETYLRLCDCWSRPVTSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H8F0</accession>
    <name>A0A9Q1H8F0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SCAN box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_17579</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8036914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H8F0"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF13">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCAN box" evidence="3">
        <location>
            <begin position="227"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="321"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="585"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="84"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="321"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="336"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="360"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="585"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="629"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="654"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="677"/>
            <end position="689"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8036914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="702" mass="79012" checksum="03BD7DE8806545D6" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HAG3</accession>
    <name>A0A9Q1HAG3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Electron transfer flavoprotein subunit alpha</fullName>
            <shortName evidence="7">Alpha-ETF</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_19178</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">The electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7 8">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="7 8">Binds 1 FAD per dimer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Heterodimer of an alpha and a beta subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the ETF alpha-subunit/FixB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8038176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HAG3"/>
    <dbReference type="OrthoDB" id="1715808at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045251">
        <property type="term" value="C:electron transfer flavoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01715">
        <property type="entry name" value="ETF_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000041">
        <property type="entry name" value="Electron transfer flavoprotein alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1220:FF:000001">
        <property type="entry name" value="Electron transfer flavoprotein, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014730">
        <property type="entry name" value="ETF_a/b_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001308">
        <property type="entry name" value="ETF_a/FixB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033947">
        <property type="entry name" value="ETF_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014731">
        <property type="entry name" value="ETF_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018206">
        <property type="entry name" value="ETF_asu_C_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43153">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43153:SF1">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01012">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00766">
        <property type="entry name" value="ETF_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000089">
        <property type="entry name" value="Electra_flavoP_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00893">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00696">
        <property type="entry name" value="ETF_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5 7 8" id="KW-0274">FAD</keyword>
    <keyword evidence="4 7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Electron transfer flavoprotein alpha/beta-subunit N-terminal" evidence="9">
        <location>
            <begin position="24"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="251"/>
            <end position="252"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="265"/>
            <end position="269"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="282"/>
            <end position="289"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="303"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000089-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8038176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="336" mass="35212" checksum="6301B79DDC099573" modified="2023-09-13" version="1">MLGLLGRNSRRFSQSVSCLRNSSTLVVAEHTNDALTPITLNTVTAATKVGGDVSCLVAGTNCSKVVEELTKVPGVTKILVADNESFKGFLPEALTPLLVNAQKQFNFTHIAAGATAFGKSLLPRLAAKLDVSPISDIISIESEDTFVRTIYAGNAIATVKSKDAVKVFTVRGTAFEASPTSGGTAQSEEAPGVDVKNELSTWEGQSLSKSDRPELTSAETVISGGRGMKNGENFKMLYELADLMNAAVGASRAAVDAGYVPNDMQVGQTGKIVAPQLYIAVGISGAIQHLAGMKDSKVIAAINKDPEAPIFQVADVGLVADLFKAVPELTEALKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HAK7</accession>
    <name>A0A9Q1HAK7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_16400</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HAK7"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="120"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="179"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8038850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="194" mass="22077" checksum="C9176A464AD84423" modified="2023-09-13" version="1">MAAQLPPMHPYNIMLYRVSVFLKADLEKVLALGRLMGCRDLQKVTNPWMLFEKLEDKGCLSEFKVVDLKNSLELIGLSEAAHAVMEYEDKELKGKSQTSSSLPPPPRYSFIKRFDETHRSVQENGLSQESECTKPLSSHEGLASDISNVPTTPMDTEKSQETRRRFKGKSHTIAAPSMEKYKERKMNGWEPTKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HBB7</accession>
    <name>A0A9Q1HBB7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_13814</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8039720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HBB7"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8039720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="183" mass="21602" checksum="51737544497238B4" modified="2023-09-13" version="1">MPFNLDKFVASPSVEELDSLKKSEIVKVAKHYGIEFQPLMRKDEIKRYVLEYLVDEGVLPSTVLETAITVPTDNTFELKRLELEMNKEIRLKEMERKKRKEKCKCKGEKEEREERERREEKAREHEFRLKQLELGGIKGSDPKIGLDTGGFDVSKHVKFVPKFQEDNVEKFFNHFEKLGEQLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HBN0</accession>
    <name>A0A9Q1HBN0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Alpha-2-macroglobulin-like protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_17289</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HBN0"/>
    <dbReference type="OrthoDB" id="9998011at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02897">
        <property type="entry name" value="A2M_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.130.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050473">
        <property type="entry name" value="A2M/Complement_sys"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041813">
        <property type="entry name" value="A2M_TED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047565">
        <property type="entry name" value="Alpha-macroglob_thiol-ester_cl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011626">
        <property type="entry name" value="Alpha-macroglobulin_TED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001599">
        <property type="entry name" value="Macroglobln_a2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019742">
        <property type="entry name" value="MacrogloblnA2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11412">
        <property type="entry name" value="MACROGLOBULIN / COMPLEMENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11412:SF171">
        <property type="entry name" value="PREGNANCY ZONE PROTEIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00207">
        <property type="entry name" value="A2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07678">
        <property type="entry name" value="TED_complement"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01360">
        <property type="entry name" value="A2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01419">
        <property type="entry name" value="Thiol-ester_cl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00477">
        <property type="entry name" value="ALPHA_2_MACROGLOBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0722">Serine protease inhibitor</keyword>
    <feature type="domain" description="Alpha-2-macroglobulin" evidence="5">
        <location>
            <begin position="56"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8039538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="610" mass="68298" checksum="8D7211F82B19A751" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HDD6</accession>
    <name>A0A9Q1HDD6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">BolA-like protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_12841</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Acts as a mitochondrial iron-sulfur (Fe-S) cluster assembly factor that facilitates (Fe-S) cluster insertion into a subset of mitochondrial proteins. Probably acts together with NFU1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Interacts with NFU1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the BolA/IbaG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HDD6"/>
    <dbReference type="OrthoDB" id="203381at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.90:FF:000003">
        <property type="entry name" value="BolA family member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.90">
        <property type="entry name" value="BolA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002634">
        <property type="entry name" value="BolA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036065">
        <property type="entry name" value="BolA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052275">
        <property type="entry name" value="Mt_Fe-S_assembly_factor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46188">
        <property type="entry name" value="BOLA-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46188:SF1">
        <property type="entry name" value="BOLA-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01722">
        <property type="entry name" value="BolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82657">
        <property type="entry name" value="BolA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00058025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00065046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00068232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8041910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="107" mass="11863" checksum="AC1673309DA37772" modified="2023-09-13" version="1">MLRQFFQRNVYPLLRTRWLSANTSGTEGEVKLTSILKEKFPKASAISVEDISGGCGAMYQIHIESVDFEGKRLVAQHKLVTQALSEEVKEMHGLRISTAVPVESGKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HDU6</accession>
    <name>A0A9Q1HDU6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Engulfment and cell motility protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_12494</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in association with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HDU6"/>
    <dbReference type="OrthoDB" id="28413at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017124">
        <property type="term" value="F:SH3 domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048870">
        <property type="term" value="P:cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006909">
        <property type="term" value="P:phagocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.810">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024574">
        <property type="entry name" value="ELMO_ARM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006816">
        <property type="entry name" value="ELMO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050868">
        <property type="entry name" value="ELMO_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12771:SF56">
        <property type="entry name" value="CED-12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12771">
        <property type="entry name" value="ENGULFMENT AND CELL MOTILITY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11841">
        <property type="entry name" value="ELMO_ARM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04727">
        <property type="entry name" value="ELMO_CED12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16457">
        <property type="entry name" value="PH_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51335">
        <property type="entry name" value="ELMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="2" id="KW-0581">Phagocytosis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0729">SH3-binding</keyword>
    <feature type="domain" description="ELMO" evidence="6">
        <location>
            <begin position="331"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="705"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="705"/>
            <end position="717"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8041623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="724" mass="82396" checksum="A70F8DAF00583B6F" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HE12</accession>
    <name>A0A9Q1HE12_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PDZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_12630</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HE12"/>
    <dbReference type="OrthoDB" id="10681657at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8041731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="359" mass="40695" checksum="FD79E33B9CF9B197" modified="2023-09-13" version="1">MANSENDSIFKACDQKCTYVDLKGKLRKDQISFSSSCLSSEASSQKGTYVDLKGKLKKDERAPRNEQKKIRFFFVDVIEGGPLHQSGIRDGDELTTVNYAEVKSFHGIYHIASAIQECSSAIMLGMRRRSRRRRRIGSVPFVVLFQLGDEDANPVYKFSGFYWNVLPRDIPFIPSCCKCDDIVSEPCPQCVTRPSVEVVIKERNEENWLTFNSSTEDWTFERKRAPTFKPELCSTFQLEVCSPAVPNSYTTVAFRPQNSLSLALEATRDNELKTSSFNCNEKGQFFNEVPLGGRLEVNIQSALTNWYLGKDKDDNAVLLQDKTPICLYNTSFKACLCVKCAETLSLTIYYQDVSKPQQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HES8</accession>
    <name>A0A9Q1HES8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Myosin light chain kinase, smooth muscle</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_10861</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HES8"/>
    <dbReference type="OrthoDB" id="6157510at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8043664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="186" mass="20968" checksum="F8DC2AC8ADD8CE57" modified="2023-09-13" version="1">MVSYPRGANKHGLTNLPQATLKQSPTKTTHPNPPHTQRQHHARGQHHARRQHHARRQHHARRQHDARREPRPKATLSPKATLSPKATLRPKATLRPKATLRPKATLRPKATLRPKATLRPKATLRPKATLRPKATLRPKATLRPKATLRPKATLRPKATALKKHRGKYRCTLPKGAPEAVVAKGWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HEY5</accession>
    <name>A0A9Q1HEY5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Serine/arginine-rich splicing factor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_09456</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8042646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HEY5"/>
    <dbReference type="OrthoDB" id="1099063at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050374">
        <property type="entry name" value="RRT5_SRSF_SR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23003">
        <property type="entry name" value="RNA RECOGNITION MOTIF RRM DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23003:SF62">
        <property type="entry name" value="SERINE_ARGININE (SR)-TYPE SHUTTLING MRNA BINDING PROTEIN NPL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="26"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="141"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="100"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="105"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8042646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="329" mass="36309" checksum="771F4D70AA4B51D6" modified="2023-09-13" version="1">MSYFDRRGGGGGGGGGRYKGGGGNDCRIYVGNLPPDIREKDIIDIFYSYGNILKVDLKRNTHGVGPPFAFVEFEDPRDAEDAKKGKDRFDYDGYNLRVEYPKSSGRGGGGSSYGGGGGGSDRGRSFGGGRPKGPPPRRSDYRCFVSGLPPTGSWQDLKDHMREAGDVCFTDVYEDGTGVVEFLRREDMEYACNTLDDSKFRSHEAVAFEAMKFSNREVWGEIPGRVKVRVRPLSSGSKVMTQVHPIREAGPGATLQKEEDERKARHIIQDTIGQDPGRGHAPDDIHTLDPALDHEIDLVWCCDVIPKMNQVDMDSNLLPSLELCLWCVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HIC2</accession>
    <name>A0A9Q1HIC2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">RNA-directed DNA polymerase from mobile element jockey</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_04585</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8051132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HIC2"/>
    <dbReference type="OrthoDB" id="6776162at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33332">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8051132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="226" mass="26343" checksum="B5CCFAA84EE3D50E" modified="2023-09-13" version="1">MVFNNRCTYKFDISIDGRCLKLVNSVKFLGVYIDSKLSWSDHIFNTSSHIARAVGILGRLKYLLPRNVLRTIYLTLIYPHLTYCSGIWSTASHCQLNKLHILQKRAIRHITCSKPRDHTNDLFKSLNLLKLPDIIKLGIASFTYRSLNNILPDYFSDYFSHNSSIHDYNTRQSTFLHQDTIQTSSQHSSLRNRAIKLWNSLPYSITHTPSIQSFRRNFSRHFIGTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HJF7</accession>
    <name>A0A9Q1HJF7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">UDP-D-xylose:L-fucose alpha-1,3-D-xylosyltransferase 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_06248</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 77 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HJF7"/>
    <dbReference type="OrthoDB" id="1712432at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005069">
        <property type="entry name" value="Nucl-diP-sugar_transferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052636">
        <property type="entry name" value="UDP-D-xylose:L-fucose_XylT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47032">
        <property type="entry name" value="UDP-D-XYLOSE:L-FUCOSE ALPHA-1,3-D-XYLOSYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47032:SF1">
        <property type="entry name" value="UDP-D-XYLOSE:L-FUCOSE ALPHA-1,3-D-XYLOSYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03407">
        <property type="entry name" value="Nucleotid_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleotide-diphospho-sugar transferase" evidence="2">
        <location>
            <begin position="83"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8047281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="290" mass="34257" checksum="095432F04B195F8D" modified="2023-09-13" version="1">MKLPPVLNNQRDNMEPVFWENGVTKIATNEKTLRSNESYQFVNFTDSVRTLTSSVLLVTTNYGFYNLTLNLLYSIQRLFIQPTILILCEDKTVYVELWKRQIKFSLKYQLALTHLEESDTIAASRNTPTYYRIMQKRISYWEILLQRGVDVFFVDSDIVLLANPFKYFTDDNSDIFVQRDGGTACAGFFYMKANNSSKNFVEAWKRALPKDPKGNQKTFNKVIKELGKNIKVNRLPKELFMSGFEYNKFQKAWWHWWPTPQPVEVHANFMIGKDTKENWLKKYKLWFVPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HJI8</accession>
    <name>A0A9Q1HJI8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Short-chain collagen C4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_06281</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HJI8"/>
    <dbReference type="OrthoDB" id="6086925at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005581">
        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051077">
        <property type="entry name" value="Ca-dependent_lectin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24024">
        <property type="entry name" value="PULMONARY SURFACTANT-ASSOCIATED PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24024:SF18">
        <property type="entry name" value="SHORT-CHAIN COLLAGEN C4-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0176">Collagen</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040305034" evidence="2">
        <location>
            <begin position="20"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8047308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="332" mass="36160" checksum="D286DEE24E833D8B" modified="2023-09-13" version="1">MFVLQTAFVILLIITATSSFGDFRVGENGKDHMEALKLNKLEGSRTYLQTKSKNGLLSEKPKRLRRSISDGAQMPGNVCSGCSSSYTQYFSGQCFLCPPGNPGPRGQPGQQGIPGRDGRDGRDAINERGDDGMGGTPHSAVRSNQTGGAVYVRWGRNECPQESQLIYEGIAGGSFYTHGGSGSNYLCMPEDPIYDEPVAGTSDQSRGYVYGAEYQASNFANWNQIHDHNVYCAVCWTKNRPSIMMIPARNICPGSEWTKEYSGYLVSAYYDHGGRTEYVCMDRNAEAVPRSSYNANGALFYPVESRCASSGSGLPCGPYVDGYELTCAVCTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HKF4</accession>
    <name>A0A9Q1HKF4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_01747</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HKF4"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8049140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="77" mass="8850" checksum="54C6CBEBA3DDE4C4" modified="2023-09-13" version="1">MPFNLDKFVASPSVEELDSLKKSEIVKVAKHYGIEFQPLMRKDEIKRYVLEYLVDESILPSTVLETAITVQLTTHLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HLK9</accession>
    <name>A0A9Q1HLK9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_03922</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HLK9"/>
    <dbReference type="OrthoDB" id="10036512at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33053">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33053:SF24">
        <property type="entry name" value="TRANSPOSASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8050640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="224" mass="25795" checksum="D36EAD7DE451D185" modified="2023-09-13" version="1">MQGPLSCRVSANFLRVISERLVMLQQSFSREFARKPRSLFEVKHWKATEFRTFLLYTGPVVLHGTIPGALYRNFLTLSVAMRILLNPAISAQYLQYAEDLLKVFVQNFGTVYGKQYLVYNVHSLIHLGDDARKFGALDDISAFPFESFLGHLKKLVRGPHYPLSQIVHRIIEKQELLCNTENVKSVQLHKKPHLHGPLPREYCHCKQCRQYCGQLFVPLSASHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HMG0</accession>
    <name>A0A9Q1HMG0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_04608</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8051148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HMG0"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8051148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="75" mass="8361" checksum="E1DCEDD48CCDCAC3" modified="2023-09-13" version="1">MRIVRAKDPLMTLSQSEGSLNQVYYSNMATSMSSTQNVRSGQNFVPCTSFHGRKVNLKPSQKTHGIYLHDMPSNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0Y9R1</accession>
    <name>A0A9Q0Y9R1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_43169</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000222">
        <property type="protein sequence ID" value="KAJ8018698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0Y9R1"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8018698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="188" mass="21546" checksum="F700BAC869141985" modified="2023-09-13" version="1">MSGTNPFDDSLTSDRVDWVQREEGRQLEETSSVWSEERTPLLPRYREDCGSQRPCNEQDEVRGSAALREDGVAPGGDALRGFGGTTLRETITIKTGTDAMQLSAQGWPVYPDRCQQQNHDGYRLKENFWGRPGEYTRLPREPDVGEYNRVRFPSTEHRNQFGTSNTHRAPVPTFDGTMPWRDYLVQFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YC14</accession>
    <name>A0A9Q0YC14_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_43225</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000238">
        <property type="protein sequence ID" value="KAJ8018671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YC14"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="44"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8018671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="98" mass="11255" checksum="5857858DF022EDD1" modified="2023-09-13" version="1">MSPRSANKITRREMTAEILQSLLESRVLRDLICSAVSDSIKSNLDCLKKAIERQEGRIHQLECELEESEKQLKKCTEENTELQSKMGELGYKLNSLEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YCI6</accession>
    <name>A0A9Q0YCI6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">1,4-alpha-glucan-branching enzyme</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_42019</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8020143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YCI6"/>
    <dbReference type="OrthoDB" id="196493at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003844">
        <property type="term" value="F:1,4-alpha-glucan branching enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004193">
        <property type="entry name" value="Glyco_hydro_13_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43651">
        <property type="entry name" value="1,4-ALPHA-GLUCAN-BRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43651:SF3">
        <property type="entry name" value="1,4-ALPHA-GLUCAN-BRANCHING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02922">
        <property type="entry name" value="CBM_48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="283"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="386"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="446"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 13 N-terminal" evidence="2">
        <location>
            <begin position="67"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8020143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="614" mass="70774" checksum="BC7CA53FF9488246" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YGK5</accession>
    <name>A0A9Q0YGK5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein RER1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_38882</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in the retrieval of endoplasmic reticulum membrane proteins from the early Golgi compartment.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the RER1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YGK5"/>
    <dbReference type="OrthoDB" id="448250at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006621">
        <property type="term" value="P:protein retention in ER lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004932">
        <property type="entry name" value="Rer1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10743">
        <property type="entry name" value="PROTEIN RER1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10743:SF0">
        <property type="entry name" value="PROTEIN RER1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03248">
        <property type="entry name" value="Rer1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016013">
        <property type="entry name" value="AtER_Rer1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="64"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="145"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR016013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8021624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="193" mass="22724" checksum="48532F42EA1A22EE" modified="2023-09-13" version="1">MEADSSDVVSSQPGFLTRLFTGIGQRYQSILDKSVPHTAARWIFTFLLELVYLARVFFLQGWYIVTYALGIYQLNLFIAFLTPKMEPVITEDTEDGPTLPTKANEEFRPFIRRLPEFKFWYSASKSIVIASICTCFDIFNVPVFWPILVMYFFILFFLTMRRQIEHMIKYRYLPWTRGKTKYKGKEDTGDVIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YGU1</accession>
    <name>A0A9Q0YGU1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein Wnt</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_39068</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Ligand for members of the frizzled family of seven transmembrane receptors.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Secreted</location>
            <location evidence="1 10">Extracellular space</location>
            <location evidence="1 10">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the Wnt family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YGU1"/>
    <dbReference type="OrthoDB" id="5945655at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005109">
        <property type="term" value="F:frizzled binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045165">
        <property type="term" value="P:cell fate commitment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19336">
        <property type="entry name" value="Wnt_Wnt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2460.20:FF:000001">
        <property type="entry name" value="Wnt homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2460.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005817">
        <property type="entry name" value="Wnt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043158">
        <property type="entry name" value="Wnt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018161">
        <property type="entry name" value="Wnt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12027:SF101">
        <property type="entry name" value="PROTEIN WNT-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12027">
        <property type="entry name" value="WNT RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00110">
        <property type="entry name" value="wnt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01349">
        <property type="entry name" value="WNTPROTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00097">
        <property type="entry name" value="WNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00246">
        <property type="entry name" value="WNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 10" id="KW-0879">Wnt signaling pathway</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8021774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="356" mass="39637" checksum="8C08AF3DF9A05FE1" modified="2023-09-13" version="1">MWATTCTHLVLTILFIITSYCVRVNHAWLSMFNIQAIGINSIENTETCEIIPGLVNRQVAICKRNLEVMDSVSKGASISILECQKQFHNRRWNCSTVDPYTVFGPVLDNGTREAAFVSSVTAAGVAHAVTRSCSLGELLKCGCDRTVSGTSPEGFTWSGCSDNVAYGIQFSKMFVDARESKSRVSVDRRLMNLHNNEAGRRAIEDNMKTECKCHGVSGSCEVKFCWRSMPSFRKVGSVLKEKFDGATEVAQQRISSRRQLVPVNPNYKPHTNSDLVYLQNSPDFCERNLAIGSLGTQNRTCNKDSKAIDGCELLCCGRGYNTKIETVSERCSCKFHWCCVVKCKTCTRTVEVYTCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YHA0</accession>
    <name>A0A9Q0YHA0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_37550</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YHA0"/>
    <dbReference type="OrthoDB" id="5915502at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38640">
        <property type="entry name" value="GEO09659P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38640:SF1">
        <property type="entry name" value="GEO09659P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="119"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8022598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="149" mass="16592" checksum="954B5F90D833ACA2" modified="2023-09-13" version="1">MSNARKEPNDDSRIGKFLDEVCFTYMPVIGAASHTIFAVHLLDKSLMPSLFPKWHFGVANGFLFNAHLGVGLYLYNRRSIARAPLPLRILWSVYGSAMFNFGSVLLFSTGKLLLVNDRLIGTLYAIAASLSFLVVGKQYLEFVDRSIDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YHH0</accession>
    <name>A0A9Q0YHH0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Microsomal triglyceride transfer protein large subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_37655</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YHH0"/>
    <dbReference type="OrthoDB" id="5865932at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005548">
        <property type="term" value="F:phospholipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042157">
        <property type="term" value="P:lipoprotein metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.230.10">
        <property type="entry name" value="Lipovitellin, beta-sheet shell regions, chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.20">
        <property type="entry name" value="Vitellinogen, superhelical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015819">
        <property type="entry name" value="Lipid_transp_b-sht_shell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011030">
        <property type="entry name" value="Lipovitellin_superhlx_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045811">
        <property type="entry name" value="MTP_lip-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039988">
        <property type="entry name" value="MTTP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015816">
        <property type="entry name" value="Vitellinogen_b-sht_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001747">
        <property type="entry name" value="Vitellogenin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13024:SF0">
        <property type="entry name" value="MICROSOMAL TRIACYLGLYCEROL TRANSFER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13024">
        <property type="entry name" value="MICROSOMAL TRIGLYCERIDE TRANSFER PROTEIN, LARGE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19444">
        <property type="entry name" value="MTP_lip_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01347">
        <property type="entry name" value="Vitellogenin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00638">
        <property type="entry name" value="LPD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56968">
        <property type="entry name" value="Lipovitellin-phosvitin complex, beta-sheet shell regions"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48431">
        <property type="entry name" value="Lipovitellin-phosvitin complex, superhelical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51211">
        <property type="entry name" value="VITELLOGENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040268985" evidence="6">
        <location>
            <begin position="17"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="domain" description="Vitellogenin" evidence="7">
        <location>
            <begin position="19"/>
            <end position="640"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8022687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="887" mass="97042" checksum="73E4201A51DD1452" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YIG3</accession>
    <name>A0A9Q0YIG3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_40585</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YIG3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="108"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="175"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="209"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="274"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="402"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8020877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="425" mass="49627" checksum="D0BB7C227D696470" modified="2023-09-13" version="1">MPMYIVWTMEYNRVLPDTQGLHRRVPPHLIIVMWCLGVFKSSQPFDYGYQDNWKLSPVRGTEISKRRPNRVLTLSSTAVPFTLMTCSLSLAVLYLFCYFGRLDHYLRFVSFFICYWLTMVITQVIFTTASLKNLCKNSSSAYTDDIRWHNALNGTAFLRNLSVQINSHRGPWPNLFTFGFFIVFASFVALINIYFFTLLEEKCRNWTGYIWLVCDTLNLMFYGMSCYFLYLQRTSLQAIGKKVAVCVGENIVNFDYCTNQIKKFFREYLQLRKIILPWLRIIIFTSSFGLTVFFTWNYEENKYLSPSVLNDNQLNATFICSYACDKASFLKERNVLLFYNVLVISRNLMIAILALTAVGGFDIAYIWERFNMRLHFLTTASEGEQWKRLINFVKKLHPSTHAAVIFSFVIPLLGFGSAFLSNKHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YJJ8</accession>
    <name>A0A9Q0YJJ8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Kinesin-like protein KIF18A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HOLleu_35923</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Nanhai2018</strain>
            <tissue evidence="15">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YJJ8"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01370">
        <property type="entry name" value="KISc_KIP3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000027">
        <property type="entry name" value="Kinesin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968">
        <property type="entry name" value="CENTROMERE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968:SF65">
        <property type="entry name" value="KINESIN MOTOR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8 11" id="KW-0505">Motor protein</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="14">
        <location>
            <begin position="36"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="672"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1018"/>
            <end position="1052"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="396"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="24"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="735"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="787"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="803"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="140"/>
            <end position="147"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ8023469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1080" mass="121455" checksum="A97B82026AF0B256" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YNL0</accession>
    <name>A0A9Q0YNL0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_33349</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YNL0"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8025717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="612" mass="69765" checksum="9BA1B06D17411C22" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YP07</accession>
    <name>A0A9Q0YP07_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_33634</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YP07"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8025927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="96" mass="11186" checksum="5DC4B9D87239C3E2" modified="2023-09-13" version="1">MDISDLIPDITASPKFTTYSEEEKITCGQRTPPSKKDRILLKEENYRKVRQHADTLSNIQATIALTEESKSVNKHLEKRTVPQHLRVYRNRPRLPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YQV1</accession>
    <name>A0A9Q0YQV1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Photoreceptor-specific nuclear receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_31864</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YQV1"/>
    <dbReference type="OrthoDB" id="5771769at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.565.10:FF:000011">
        <property type="entry name" value="Nuclear receptor subfamily 5, group A, member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035500">
        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050274">
        <property type="entry name" value="Nuclear_hormone_rcpt_NR2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001723">
        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001628">
        <property type="entry name" value="Znf_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24083">
        <property type="entry name" value="NUCLEAR HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00105">
        <property type="entry name" value="zf-C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01282">
        <property type="entry name" value="COUPTNFACTOR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00398">
        <property type="entry name" value="STRDHORMONER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00430">
        <property type="entry name" value="HOLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00399">
        <property type="entry name" value="ZnF_C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48508">
        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51843">
        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51030">
        <property type="entry name" value="NUCLEAR_REC_DBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Nuclear receptor" evidence="10">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="NR LBD" evidence="11">
        <location>
            <begin position="110"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="39"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8026902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="339" mass="38304" checksum="DF751BEBA969F261" modified="2023-09-13" version="1">MYTCKQEGKGTCPIDKTHRNQCRACRLKKCFEAEMNKDAVQHERGPRKPKNKPMNEMPTSPSLQELRSGRPMAPAMPEPRHGSTPPSPPITLVSSSMYYGTFYHSLLTAEQCNVSPMALDLSSKRDGKSAIPLRVEATPGNGGPSECLPEVAARLLFSSVKWAKSIPCFRLLPFRDQLILLEEGWRDLFLLGVCQWGVPLDTESLLKAASPKLDKLSKDELRDLEVQIRHVQDVVVRVRAMKIDFTEYACIKAIALFKPDTPGLREPYTVESFQDQAQVMLNDYERYANISRPMRFGRLLLSLPALRGVSSKFLENLFFKQTIGNIPIERLLSDMFQNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YTT7</accession>
    <name>A0A9Q0YTT7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Charged multivesicular body protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_33775</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SNF7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8026041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YTT7"/>
    <dbReference type="OrthoDB" id="2329734at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1230">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005024">
        <property type="entry name" value="Snf7_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10476">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03357">
        <property type="entry name" value="Snf7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="133"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="158"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8026041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="168" mass="18763" checksum="F18762F3B3C7D43E" modified="2023-09-13" version="1">MGLFGKEQKKDPKEQVQEWTRALNKQSRGLDRQIRSIEREEAKVKRSLKDAAKQGHKDVCTTLAKEIVRSRKAKNRIYASKAQINSAGIMEEMVEDTFESLEDQDELEDAAQEEVDQVLSELTAGALANVPSAVTDDLPEAAGATAISDESDEEEAEDMQRRLEALRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YUE4</accession>
    <name>A0A9Q0YUE4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Adenosine receptor A2a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_31897</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YUE4"/>
    <dbReference type="OrthoDB" id="9046662at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45698:SF1">
        <property type="entry name" value="TRACE AMINE-ASSOCIATED RECEPTOR 13C-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45698">
        <property type="entry name" value="TRACE AMINE-ASSOCIATED RECEPTOR 19N-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="28"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="64"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="7">
        <location>
            <begin position="44"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8026926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="108" mass="12208" checksum="C4A7EEF122C6870B" modified="2023-09-13" version="1">MESVTEGQAENDPNGGWSSDQDSFDDAIWIRAAYGIIAVLGILGNFLVIFTIARVKALQNLTNFFIACLAATDFITSVFLIPLNMGKYYIYFFCIYFKCYVFELRKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1B986</accession>
    <name>A0A9Q1B986_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_44694</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010001125">
        <property type="protein sequence ID" value="KAJ8017705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1B986"/>
    <dbReference type="OrthoDB" id="6149057at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8017705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="430" mass="48559" checksum="1B6EE6D6A24BBF38" modified="2023-09-13" version="1">MLNLTGSTTIHYETVENRLHLLLPLNAEMFLLRMLHRINSFIGYNHTSKLCSLGNARHSKGEDVQCPGHVGCSATVSPDFGIGNEEDGGEKIASKLLAGKHPLKVSHLTTDADGKGCQGLNKVMKRVTGKDTENLLDEQHLNRSFCRAVSSTEFSSDMFPARTVSFKRMLQKRFAEDLSHQVQAEITACRKAMKNNHPKVVLAMRDCVSCILKCYSGDHRECSKKSFVCKKKKYMFPFMPGIARESLRILGTDRVKLCNVIMTRTCEKQLRATRFGTSTQKAEAMNSAFKTTNPKQGSTYSRNASCRDHSAIHMVNNGPGESIAMKMAALGLHPSQSSISTLRRLQHRRDYHRRRKKSCSVRARKASLRIKRYRLYERQGNYRKNKGSCYETDQLIAELTDHTYHKRRISCDNSGNDITSCGTSRNVRTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1B9G7</accession>
    <name>A0A9Q1B9G7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Neurotrypsin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_44103</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000640">
        <property type="protein sequence ID" value="KAJ8018095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1B9G7"/>
    <dbReference type="OrthoDB" id="9119369at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19331">
        <property type="entry name" value="SCAVENGER RECEPTOR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00530">
        <property type="entry name" value="SRCR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00258">
        <property type="entry name" value="SPERACTRCPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="SRCR" evidence="5">
        <location>
            <begin position="40"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="5">
        <location>
            <begin position="147"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="5">
        <location>
            <begin position="262"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="66"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="215"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="331"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8018095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="366" mass="40980" checksum="C711C956E4976ACE" modified="2023-09-13" version="1">MLCYLHSFSQWSLFKVSTYTENALPLGIGVGYWADGDALLRLVGGNSSLEGRLEVSWGGGVWGSVCSKFWGLGSARFVCRHLGYTGVEDYGTVEGNASYPMLQYLSCNDEDETYWDDCLHRYTRPEKIFCDSYATVKCSAFANEGTLRLTGAAPAREGRVEIFRDGVWGGVSIRDDNAHAVVCNQLGYPGRHRILDNIEFEVSVNSPVLLDIIDCQGSEASIIDCVHVWYNHSDASYNKGANTRCNRPATVTAFSLKGNFAPRLYNGTSIYEGRLEIWEGDQWRRVCNSTIGPNEAEVICRQLGNMSLETFYNWPKFGKGINPVLSWSFSCNRMVTFLNQCDYSYVTTRNLDCDSVAVKCFSELKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1B9V0</accession>
    <name>A0A9Q1B9V0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Neuronal acetylcholine receptor subunit alpha-10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_45218</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010002123">
        <property type="protein sequence ID" value="KAJ8017403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1B9V0"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005230">
        <property type="term" value="F:extracellular ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0675">Receptor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="133"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="166"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="194"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="270"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="4">
        <location>
            <begin position="5"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8017403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="292" mass="34387" checksum="CFD149ADB20CC8FE" modified="2023-09-13" version="1">METDYVKLFSNGTIYWYTLAMTTSYCRVNVKHFPFDKQKCVLTFTTWLYQSTERSYQKSNKVKIFFDLQQAFYIIDDIIKNEMYKNYEVSSGLWVWDITDLHAGNNSYYCSTCNNQEQSYITYTVEMTRTEHVWHFLIYLLPCMVLSTMLILIVELTRRYDVDAPNFGLTCILTLFVFLTFLFTQLPATGPPIMGFYIVVLIVEAVSATVYVTFRKAPRSCTKDDHIALADRKRDNKTLTEDTEDEKSKTTVTRMKTGWMGWIDKWIIRWWCFTGINMVFVIIFFPVIGFGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-04-02" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BBY8</accession>
    <name>A0A9Q1BBY8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Rab effector MyRIP</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_41670</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8019893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10072397at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030864">
        <property type="term" value="C:cortical actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017022">
        <property type="term" value="F:myosin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000018">
        <property type="entry name" value="Synaptotagmin-like 5, isoform CRA_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041282">
        <property type="entry name" value="FYVE_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051745">
        <property type="entry name" value="Intracell_Transport_Effector"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010911">
        <property type="entry name" value="Rab_BD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14555">
        <property type="entry name" value="MYELIN-ASSOCIATED OLIGODENDROCYTIC BASIC PROTEIN MOBP -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14555:SF3">
        <property type="entry name" value="RABBD DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02318">
        <property type="entry name" value="FYVE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50916">
        <property type="entry name" value="RABBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RabBD" evidence="2">
        <location>
            <begin position="4"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="147"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="221"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="454"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="509"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="640"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="928"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1006"/>
            <end position="1038"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1053"/>
            <end position="1110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="153"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="184"/>
            <end position="195"/>
        </location>
    </feature>
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        <location>
            <begin position="252"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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            <begin position="285"/>
            <end position="319"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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            <end position="347"/>
        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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            <begin position="373"/>
            <end position="392"/>
        </location>
    </feature>
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        <location>
            <begin position="459"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
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            <begin position="511"/>
            <end position="524"/>
        </location>
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    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
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            <end position="562"/>
        </location>
    </feature>
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            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
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            <begin position="584"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="595"/>
            <end position="611"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="640"/>
            <end position="651"/>
        </location>
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        <location>
            <begin position="681"/>
            <end position="700"/>
        </location>
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        <location>
            <begin position="701"/>
            <end position="712"/>
        </location>
    </feature>
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        <location>
            <begin position="824"/>
            <end position="843"/>
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        <location>
            <begin position="851"/>
            <end position="867"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
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            <end position="941"/>
        </location>
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        <location>
            <begin position="1022"/>
            <end position="1035"/>
        </location>
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        <location>
            <begin position="1062"/>
            <end position="1072"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1080"/>
            <end position="1103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8019893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1463" mass="160604" checksum="BB0D4E931F284692" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BC83</accession>
    <name>A0A9Q1BC83_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Nuclear receptor subfamily 4 group A member 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_41672</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the nuclear hormone receptor family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8019894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BC83"/>
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    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:nuclear glucocorticoid receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:cellular response to corticotropin-releasing hormone stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="nuclear receptor subfamily 4 group A member 2"/>
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        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
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    </dbReference>
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        <property type="entry name" value="Retinoid X Receptor"/>
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        <property type="entry name" value="NR4A1-3"/>
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        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
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        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
    </dbReference>
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        <property type="entry name" value="Znf_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR24085">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NUCLEAR HORMONE RECEPTOR HR38-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="zf-C4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NUCLEARECPTR"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="STROIDFINGER"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00399">
        <property type="entry name" value="ZnF_C4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NUCLEAR_REC_DBD_1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="8 10 14" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Nuclear receptor" evidence="12">
        <location>
            <begin position="401"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="domain" description="NR LBD" evidence="13">
        <location>
            <begin position="501"/>
            <end position="736"/>
        </location>
    </feature>
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            <begin position="82"/>
            <end position="114"/>
        </location>
    </feature>
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            <begin position="145"/>
            <end position="164"/>
        </location>
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            <end position="226"/>
        </location>
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            <end position="501"/>
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        <location>
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            <end position="98"/>
        </location>
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        <location>
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            <end position="186"/>
        </location>
    </feature>
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        <location>
            <begin position="209"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU004334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ8019894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="736" mass="80550" checksum="86B93668EF329C5F" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BCM4</accession>
    <name>A0A9Q1BCM4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MRN complex-interacting protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_40172</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BCM4"/>
    <dbReference type="OrthoDB" id="5960226at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007095">
        <property type="term" value="P:mitotic G2 DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032739">
        <property type="entry name" value="MRNIP"/>
    </dbReference>
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        <property type="entry name" value="MRNIP_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15863">
        <property type="entry name" value="MRN COMPLEX-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MRN COMPLEX-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15749">
        <property type="entry name" value="MRNIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MRN complex-interacting protein N-terminal" evidence="2">
        <location>
            <begin position="2"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="176"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="176"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF15749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8020553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="309" mass="35669" checksum="76C5154E5E6EF2A3" modified="2023-09-13" version="1">MVYGKGSGADCRKLVQSLNMTAGELMESRRTSSNMQPNEHESTPHKEEVIRDSSEATSKWTNFLEEPEWTGFEDSNPDTKEHFNVVTERPRAIRRQHGIISFNKKQRFPEKTTHWQSSYCTKSASENVDSEKLYQQELLSLSASSSYPADVDEFDEGNDTLSSSRCRFFTRVSEDDKTSHPESEMNRCHASSKPSIEDSNLKNGVLQEKIPQQMNVSIHKQSHPMKRKRKYEAEVSKETLVNVRGNNSKWNMFLPAEMDDSEEEEKHVSEDSMERGWLKEDSTVMLDECSRSKIFQIDDALNDDLSWYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BDF6</accession>
    <name>A0A9Q1BDF6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_40192</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BDF6"/>
    <dbReference type="OrthoDB" id="10071849at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030417">
        <property type="entry name" value="MS4A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23320:SF165">
        <property type="entry name" value="MARVEL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23320">
        <property type="entry name" value="MEMBRANE-SPANNING 4-DOMAINS SUBFAMILY A MS4A -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="43"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="69"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="99"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="143"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="205"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="230"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8020567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="254" mass="27303" checksum="2AB446EFBA273A93" modified="2023-09-13" version="1">MDTSTPLVVSQSSPSSIVEIRSMRQLSVSYDTIDVDGCKHSGMIQSIIGTLSIIFGVACVAIECEQSSIAYPIWGGVWFMTTGFIGCRSVAKLRSGSHVALVAYATLNIFCGVIAFNMLVVTSQAAIEESENSEASTPETARVALDCVLVFLGSAEVCAAVWGDIVAFCAIRNIRKQRSTLLSQQTTTTYQTMGNTAVITSPFQPHPYGASGYQPPPGYDAPPKYEEVSKSQMPPSYHDNTTAEPPEQQYSESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BEL0</accession>
    <name>A0A9Q1BEL0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">E-selectin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_34960</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BEL0"/>
    <dbReference type="OrthoDB" id="8950604at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050111">
        <property type="entry name" value="C-type_lectin/snaclec_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22803">
        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0768">Sushi</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040251388" evidence="4">
        <location>
            <begin position="25"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="6">
        <location>
            <begin position="118"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="6">
        <location>
            <begin position="180"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="6">
        <location>
            <begin position="244"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="6">
        <location>
            <begin position="308"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="5">
        <location>
            <begin position="380"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="5">
        <location>
            <begin position="585"/>
            <end position="698"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8024906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="703" mass="78655" checksum="75F95A4938D6234B" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BG56</accession>
    <name>A0A9Q1BG56_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Jerky protein-like-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_34311</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BG56"/>
    <dbReference type="OrthoDB" id="10043687at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15517">
        <property type="entry name" value="PHD_TCF19_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050863">
        <property type="entry name" value="CenT-Element_Derived"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004875">
        <property type="entry name" value="DDE_SF_endonuclease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006600">
        <property type="entry name" value="HTH_CenpB_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19303:SF74">
        <property type="entry name" value="POGO TRANSPOSABLE ELEMENT WITH KRAB DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19303">
        <property type="entry name" value="TRANSPOSON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03184">
        <property type="entry name" value="DDE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03221">
        <property type="entry name" value="HTH_Tnp_Tc5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51253">
        <property type="entry name" value="HTH_CENPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH CENPB-type" evidence="3">
        <location>
            <begin position="87"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="15"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8024408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="604" mass="68672" checksum="38D8E2C14BE27C2D" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BGN5</accession>
    <name>A0A9Q1BGN5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Eukaryotic translation initiation factor 3 subunit M</fullName>
            <shortName evidence="5">eIF3m</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_36506</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CSN7/EIF3M family. CSN7 subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the eIF-3 subunit M family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BGN5"/>
    <dbReference type="OrthoDB" id="10267031at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071541">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex, eIF3m"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03012">
        <property type="entry name" value="eIF3m"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045237">
        <property type="entry name" value="COPS7/eIF3m"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027528">
        <property type="entry name" value="eIF3m"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040750">
        <property type="entry name" value="eIF3m_C_helix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15350">
        <property type="entry name" value="COP9 SIGNALOSOME COMPLEX SUBUNIT 7/DENDRITIC CELL PROTEIN GA17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15350:SF2">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18005">
        <property type="entry name" value="eIF3m_C_helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5 8" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="4 5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="7">
        <location>
            <begin position="178"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="288"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8023927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="380" mass="43885" checksum="E8C6BD97253A7B01" modified="2023-09-13" version="1">MNFTTFISLQETEQAGEIKSYLKSQGATIKETLSDKGIQEDLFEIIQAVGVVWKEHDEQDIEALFNSILSLLIYLPLEDMNILVPKVSEHLVAAGDGQMCDLRLKILSSLFHGVGEQNPLSYTVYSSMLQVAAQAKSCELLQTELEQVRDWIKIWQLAQEQTCNLLRLLYEALKESKQSEKASKVMVELLGTYTEDNASQARDEAHRCIVNALADPNVYLFDHLLTLRPVKFLEGERIHELLTIFVSGNLQMYQDFYNTNRDFIQTLALNHEDTIHKMRILTFMGMAVDQKELSFDQIEQALKMQEEEVERFIIEVVKSKMAQVKIDQKNRKVFVSYVTHRTFGKHQWQQLRDILTSWQKNLAHIRERLDTVANPPEETS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BHN5</accession>
    <name>A0A9Q1BHN5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_34214</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8026388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BHN5"/>
    <dbReference type="OrthoDB" id="10065153at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8026388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="161" mass="18276" checksum="2C627221D7008397" modified="2023-09-13" version="1">MIGTLTEEKKSHWSFFLLELTHAYNSTEHSTTGLSPFYLFGRNPSLPIDILLNSYVNDSELCEDSWVDVHYNRLKNAFTIAQKYASNVAASAKKEYDNSASDVPLLPGERVLVRTFKPRKKKKKKEKKIGDKWEAVPYIIVDQPILKNTKPSDPTDPSASN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BJU9</accession>
    <name>A0A9Q1BJU9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_30060</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8027956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BJU9"/>
    <dbReference type="OrthoDB" id="6375801at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46601:SF2">
        <property type="entry name" value="UBIQUITIN-LIKE PROTEASE FAMILY PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46601">
        <property type="entry name" value="ULP_PROTEASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8027956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="326" mass="37782" checksum="93170A956A2DFE4C" modified="2023-09-13" version="1">MGRPPAFTETQKTWIIGFLKRSNISYTCPGRKEAVYVGKSADGERQYHAKHYLLWYMKEIVPLMNEEIQKQFQPDEQFKVSYGSLVRFIKPIKYMRYRDDMPETTCTCEYCENLELILSAVKLACPETQCDGSTASLMKNVMCDPLNLACVNCECDVCGIGNEFYLNLIEDVGAVEEVTYHKWVLGGKYPEKQLHMVSGQEAADEMAKQIRDFKRHNYNNIRQHIELDNLKTSLPGTNSAIVQVDFSENYDNKQRNEIQSAYFGHQSFSIYTACVWYGCHKEVKCKSFCIISDEMSHDKFVALYCNKMLMKKIQEILPTVDTIHFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BK92</accession>
    <name>A0A9Q1BK92_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nipped-B-like protein B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_30284</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BK92"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039190">
        <property type="entry name" value="TTC14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23184:SF10">
        <property type="entry name" value="ARGININE RICH PROTEIN (AFU_ORTHOLOGUE AFUA_1G12120)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23184">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="193"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8028134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="344" mass="40690" checksum="C95A9B94E65024A4" modified="2023-09-13" version="1">MNNCLVFYPPGSIRVPQGVEGLFQHSVKLAWKLVITLEDGTEIDDDSLGLVSEEPLIFGQPEPFQLYTDDNVNSAMKREPWYSQFSNEKEKKERKKERKKERKKERKKERKKERKKERKKERKKERKKERKKERKKERKKERKKERKKERKKERKKERKKERKKERKKERKKERKKERKKERKKERKKERTGTGTGTGTGTGTGTGTGTGTGTGTGSIPRVDFLGVRIQRAVHYCQMPLQKLFCWSLLVWSLLVLLVQIKVDPQAFDHEIWPEKYLVLKVQCFAENYIYETGVAFLAISDNPVLFRDRLLANINPQGGSKAKKEGQAGRIEKLVEKVKEFEWDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BNX0</accession>
    <name>A0A9Q1BNX0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_29425</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BNX0"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63825">
        <property type="entry name" value="YWTD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8029899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="120" mass="13625" checksum="0878EF7EEDE3C271" modified="2023-09-13" version="1">MKAKADGSEGQTLVDGLNDPRTIVQDEEMFLYWVDVATSSVGRIRTDGSMEEELFNHNVFTGVHSFVLDSFTYYWSRPADKDIIDVDRHNPNLIGTLSVKDAVTMEGLYYFKSDQPIARK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BPB2</accession>
    <name>A0A9Q1BPB2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">oleoyl-[acyl-carrier-protein] hydrolase</fullName>
            <ecNumber evidence="1">3.1.2.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_26759</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.14" evidence="1"/>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="329835at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004312">
        <property type="term" value="F:fatty acid synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016297">
        <property type="term" value="F:fatty acyl-[ACP] hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05195">
        <property type="entry name" value="enoyl_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08954">
        <property type="entry name" value="KR_1_FAS_SDR_x"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000209">
        <property type="entry name" value="Polyketide synthase Pks12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1470.20">
        <property type="entry name" value="Fatty acid synthase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049391">
        <property type="entry name" value="FAS_pseudo-KR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057326">
        <property type="entry name" value="KR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013968">
        <property type="entry name" value="PKS_KR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050091">
        <property type="entry name" value="PKS_NRPS_Biosynth_Enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001031">
        <property type="entry name" value="Thioesterase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43775">
        <property type="entry name" value="FATTY ACID SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43775:SF7">
        <property type="entry name" value="FATTY ACID SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13602">
        <property type="entry name" value="ADH_zinc_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21149">
        <property type="entry name" value="FAS_pseudo-KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08659">
        <property type="entry name" value="KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00975">
        <property type="entry name" value="Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00822">
        <property type="entry name" value="PKS_KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="7" id="KW-0521">NADP</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carrier" evidence="12">
        <location>
            <begin position="979"/>
            <end position="1056"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8030362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1367" mass="152531" checksum="5980A2293182D241" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BPF3</accession>
    <name>A0A9Q1BPF3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">ATP-dependent DNA helicase PIF1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_26660</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BPF3"/>
    <dbReference type="OrthoDB" id="6141723at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051055">
        <property type="entry name" value="PIF1_helicase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47642">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47642:SF8">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0347">Helicase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8030293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="256" mass="28883" checksum="720989128B157881" modified="2023-09-13" version="1">MVGHRMFRYIDQRLQQIMGKKIVFGGVSKIAVGDLFQVKPVKDGWIFNDLTKNYGPLATNLWRSYFRAYDLTEIMRRKDDQAFAQLLNRLREGNQTSDDITVLKARQFSVDEVPKEATHLFQTNDQVNAHNDKMFHLLKTAKVEIPAIDIVTGDVSKTVKEITLNHIPKNPNLTMGLTGNMSLAVGQRVDLCLNVAVDDGLINGASGIVKCIQNVQTNQIHTIRIQFDDPSIGKTFRQSTRHLISSDLPSAWTPIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BQB0</accession>
    <name>A0A9Q1BQB0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_27367</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BQB0"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8030836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="74" mass="8554" checksum="2324A3A1D75B6775" modified="2023-09-13" version="1">MLILSTPKIEPKLVFMFPSCKCTFCTEACNELYLYLAISNVFKIILQITCLPRGNYYSKQYQGLSSLIIHQKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BT88</accession>
    <name>A0A9Q1BT88_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_25593</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BT88"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="18"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="121"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8032151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="185" mass="20859" checksum="1DAF017B2A77C9DF" modified="2023-09-13" version="1">MSEYDTESYCCCSLRDRCLIFAAQILGLFAFYDVGIFMELVKINSPESQMPSQALVDITDVVIVIFVICTVLVIFTACCIAGILKKRRWMLLVFLIAFGIYDGLQFVVVVLLVIFNPIQISTLWVMGVALFLSISVFYTWGWYSVLLLFRKMKVPASQRETAMSLIPSAKPYTDNVDTEEDGGDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BTK4</accession>
    <name>A0A9Q1BTK4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SRCR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_26136</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BTK4"/>
    <dbReference type="OrthoDB" id="536948at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00530">
        <property type="entry name" value="SRCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SRCR" evidence="4">
        <location>
            <begin position="15"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="63"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="22"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8032576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="102" mass="11651" checksum="DC7A6449C44E52B6" modified="2023-09-13" version="1">MHIYWDSPSDRYEIVWMSNVQCRGNETSISTCRHERLKEGNCSIGKYAGVTCEGKIMKRLGSYMPTQDVSKKKKKTPPPQQTRTAPICVYRNQNLSPPLSLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BU00</accession>
    <name>A0A9Q1BU00_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Interferon-induced protein with tetratricopeptide repeats 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_22899</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the IFIT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8032837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10043504at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10271">
        <property type="entry name" value="INTERFERON-INDUCED PROTEIN WITH TETRATRICOPEPTIDE REPEATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10271:SF0">
        <property type="entry name" value="INTERFERON-INDUCED PROTEIN WITH TETRATRICOPEPTIDE REPEATS 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8032837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1688" mass="195479" checksum="57E77FD0A36ACB6A" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BVD5</accession>
    <name>A0A9Q1BVD5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Eukaryotic translation elongation factor 1 epsilon-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_23708</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BVD5"/>
    <dbReference type="OrthoDB" id="19141at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017101">
        <property type="term" value="C:aminoacyl-tRNA synthetase multienzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043517">
        <property type="term" value="P:positive regulation of DNA damage response, signal transduction by p53 class mediator"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053836">
        <property type="entry name" value="Arc1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042450">
        <property type="entry name" value="EEF1E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44490">
        <property type="entry name" value="EUKARYOTIC TRANSLATION ELONGATION FACTOR 1 EPSILON-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44490:SF1">
        <property type="entry name" value="EUKARYOTIC TRANSLATION ELONGATION FACTOR 1 EPSILON-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21972">
        <property type="entry name" value="Arc1p_N_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="2" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST C-terminal" evidence="1">
        <location>
            <begin position="34"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8033460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="178" mass="20182" checksum="395C5DB1859D462E" modified="2023-09-13" version="1">MKPSEVENEVTALAKYLQVKPCKCTVDKKSGVTQLKHNGQTVCGLASIATFLVQSSGQTSLYGGPCHLSQAQIQQWLEYRVAIVDRCLGERDVALVLKELNAFLQHRVYFVGSSLSLADLLLYFGLYATINQLTFQEKEKHIHLSRWFNNIQRQVNLDEFYPQLPFSLNHLYHGVSAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BVG8</accession>
    <name>A0A9Q1BVG8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">LINE-1 retrotransposable element ORF2 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_23899</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BVG8"/>
    <dbReference type="OrthoDB" id="10072093at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8033602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="91" mass="10525" checksum="8350659F2D771C78" modified="2023-09-13" version="1">MLGFLHYNDGNDLFRLNYQPKLSRLKNTVRLWSSRDLTPIGRNTVVKSCALSQLVFLFLVLPNPPESFLKELQNIIFDFIWSGNPEAFGHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BVY7</accession>
    <name>A0A9Q1BVY7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Tenascin-R</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_24235</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BVY7"/>
    <dbReference type="OrthoDB" id="6118826at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050373">
        <property type="entry name" value="Fibrinogen_C-term_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143">
        <property type="entry name" value="FIBRINOGEN/TENASCIN/ANGIOPOEITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00186">
        <property type="entry name" value="FBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="1">
        <location>
            <begin position="40"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8033866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="239" mass="27588" checksum="D3011B0ACACAA365" modified="2023-09-13" version="1">MPCDTNENTSECYCELPNFKCANVGDGNLRHCYCKTSSGEMCAWFPQDCYELYNRGIRQCGVYTIQTTDPSQLRFSVYCNMTIDVFQRRRDGSVDFNQNWTTYEGGFGNLMGDFWLGNEKLCHITQQLKYKLRIDRFHYNYRALYSVYDNFVVLCANESYKLVGLNLLTGTLGTGEDGLINYSGKTFKTCDQHAGCECGWWCCYLGCRTNLNGQYNDGIKWAGYAKYSYIEMKMKPFST</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BW44</accession>
    <name>A0A9Q1BW44_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Counting factor associated protein D</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_24217</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BW44"/>
    <dbReference type="OrthoDB" id="65740at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02248">
        <property type="entry name" value="Peptidase_C1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000087">
        <property type="entry name" value="Counting factor associated protein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025661">
        <property type="entry name" value="Pept_asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025660">
        <property type="entry name" value="Pept_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039417">
        <property type="entry name" value="Peptidase_C1A_papain-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013201">
        <property type="entry name" value="Prot_inhib_I29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08246">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00705">
        <property type="entry name" value="PAPAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00848">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00640">
        <property type="entry name" value="THIOL_PROTEASE_ASN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00639">
        <property type="entry name" value="THIOL_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Cathepsin propeptide inhibitor" evidence="8">
        <location>
            <begin position="256"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="7">
        <location>
            <begin position="340"/>
            <end position="557"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8033850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="559" mass="63280" checksum="9744B8314A53AC12" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BWC9</accession>
    <name>A0A9Q1BWC9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">non-specific protein-tyrosine kinase</fullName>
            <ecNumber evidence="5">2.7.10.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">HOLleu_24211</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="23">Nanhai2018</strain>
            <tissue evidence="23">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Focal adhesion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the protein kinase superfamily. Tyr protein kinase family. Fes/fps subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.2" evidence="5"/>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BWC9"/>
    <dbReference type="OrthoDB" id="9976756at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004715">
        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008284">
        <property type="term" value="P:positive regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007172">
        <property type="term" value="P:signal complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13190">
        <property type="entry name" value="FERM_C_FAK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05056">
        <property type="entry name" value="PTKc_FAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000039">
        <property type="entry name" value="Focal adhesion kinase, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000004">
        <property type="entry name" value="Protein-tyrosine kinase 2-beta isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000194">
        <property type="entry name" value="protein-tyrosine kinase 2-beta isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041390">
        <property type="entry name" value="FADK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049385">
        <property type="entry name" value="FAK1-like_FERM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041784">
        <property type="entry name" value="FAK1/PYK2_FERM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036137">
        <property type="entry name" value="Focal_adhe_kin_target_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005189">
        <property type="entry name" value="Focal_adhesion_kin_target_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46221">
        <property type="entry name" value="FERM AND PDZ DOMAIN-CONTAINING PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46221:SF9">
        <property type="entry name" value="NON-SPECIFIC PROTEIN-TYROSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21477">
        <property type="entry name" value="FERM_C_FAK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00373">
        <property type="entry name" value="FERM_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18038">
        <property type="entry name" value="FERM_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03623">
        <property type="entry name" value="Focal_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68993">
        <property type="entry name" value="FAT domain of focal adhesion kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0965">Cell junction</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 23" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="15" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="FERM" evidence="22">
        <location>
            <begin position="31"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="455"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="394"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="735"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="819"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="396"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="20">
        <location>
            <begin position="743"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="854"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="487"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00051245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00061333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KAJ8033845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1049" mass="119165" checksum="6BE285B5FE205334" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BWE4</accession>
    <name>A0A9Q1BWE4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_24507</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8034082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BWE4"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8034082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="106" mass="11705" checksum="81C08448FCF69083" modified="2023-09-13" version="1">MHTWGNENRGAYLVVCSGAEEHTQCSLRSRGAYACEAYGTRGARVITLGEEDRYCEARGADKHAHMGQWEQRSIPVVCSEQRSNARSRGAYVGDIVSFVRPISLAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BWQ5</accession>
    <name>A0A9Q1BWQ5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_20890</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BWQ5"/>
    <dbReference type="OrthoDB" id="10066543at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8034165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="62" mass="7136" checksum="12F57CFF3782E70B" modified="2023-09-13" version="1">MAYVIDSDTFLNAFYRFVSRRGIPEKVLSYNGSNFVGANRELRELYQSLDKTKIQDVTANRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BY86</accession>
    <name>A0A9Q1BY86_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ATP-dependent DNA helicase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_22168</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BY86"/>
    <dbReference type="OrthoDB" id="416437at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051055">
        <property type="entry name" value="PIF1_helicase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47642">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47642:SF5">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13245">
        <property type="entry name" value="AAA_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8035072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="84" mass="8919" checksum="0915E946F073742F" modified="2023-09-13" version="1">MSFLTGGAGTGKSLLVKCIKHEAEKIFPLELPSTDATILLLAFTGTAAFNIHGQTIHLALSIQNTKMPYIPLGQGNIKHASCQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BYB8</accession>
    <name>A0A9Q1BYB8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_22135</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BYB8"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8035050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="64" mass="7267" checksum="5E597430E73EB5C3" modified="2023-09-13" version="1">MESFREFQNSLEIVIIHHRLALSLFARGTSSYATARCRSTTVPSSTCMHYQPLHQTRIILSKGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BZ30</accession>
    <name>A0A9Q1BZ30_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sushi domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_22201</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BZ30"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0768">Sushi</keyword>
    <feature type="domain" description="Sushi" evidence="3">
        <location>
            <begin position="84"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8035091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="223" mass="25377" checksum="A0E0D125AD930FC2" modified="2023-09-13" version="1">MYCISRSVIHFYFFYLSTVDIPVCFYRRSCDGRNFEVKRIDCNENKNLIYRKYPDKTDFCMKDLLLCSLQVFSHFKAGLRSPAITCDFGILPDVPNARVESSTCFNNCEGDVCPVTCEENYYVKGDNTAYCTNVNNKGQWEVSNFMCEEITCPNDEKDFPPVFGGQFKKTNCSGNKVGHTCTMICATNFFTLEVKQTTCEKFDDSTGKWSSGVFSCNGKISNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BZU5</accession>
    <name>A0A9Q1BZU5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_19316</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8035589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BZU5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="60"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8035589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="76" mass="8809" checksum="15B2ADF4F03727E1" modified="2023-09-13" version="1">MQWPRKLDMEDGKDVRELEEDEENDKDGEVGRLSPSPPSFFRHICKLKLFYKFKSRSKSSSRSNSGSKSRSLGSLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C0C1</accession>
    <name>A0A9Q1C0C1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_20125</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C0C1"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8036212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="76" mass="8914" checksum="C2DB0764AD80D037" modified="2023-09-13" version="1">MVSLTSLLTESGINSRLHNEGFYCYKTTRQEHWIESIDERVESSREFNFNAAFLELSRSENCLRYVPIIAATWQHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C1I0</accession>
    <name>A0A9Q1C1I0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="21">Latent-transforming growth factor beta-binding protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">HOLleu_20691</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">Nanhai2018</strain>
            <tissue evidence="21">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="4405280at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007154">
        <property type="term" value="P:cell communication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000038">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000014">
        <property type="entry name" value="Latent-transforming growth factor beta-binding protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000017">
        <property type="entry name" value="latent-transforming growth factor beta-binding protein 4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000118">
        <property type="entry name" value="protein delta homolog 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000240">
        <property type="entry name" value="Vitamin K-dependent protein S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056619">
        <property type="entry name" value="C8-3_MUC4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038081">
        <property type="entry name" value="CalX-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003644">
        <property type="entry name" value="Calx_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024731">
        <property type="entry name" value="NELL2-like_EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052235">
        <property type="entry name" value="Nephronectin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003886">
        <property type="entry name" value="NIDO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001846">
        <property type="entry name" value="VWF_type-D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24050">
        <property type="entry name" value="PA14 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24050:SF28">
        <property type="entry name" value="UROMODULIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23263">
        <property type="entry name" value="C8-3_MUC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03160">
        <property type="entry name" value="Calx-beta"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12947">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06119">
        <property type="entry name" value="NIDO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="22"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00539">
        <property type="entry name" value="NIDO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00216">
        <property type="entry name" value="VWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141072">
        <property type="entry name" value="CalX-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51233">
        <property type="entry name" value="VWFD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="11 13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 13" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="15" id="KW-0768">Sushi</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="2967"/>
            <end position="2992"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="19">
        <location>
            <begin position="900"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="18">
        <location>
            <begin position="957"/>
            <end position="995"/>
        </location>
    </feature>
    <feature type="domain" description="VWFD" evidence="20">
        <location>
            <begin position="1429"/>
            <end position="1641"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="18">
        <location>
            <begin position="1826"/>
            <end position="1868"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="18">
        <location>
            <begin position="1869"/>
            <end position="1908"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="18">
        <location>
            <begin position="1909"/>
            <end position="1950"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="18">
        <location>
            <begin position="1951"/>
            <end position="1991"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="18">
        <location>
            <begin position="1992"/>
            <end position="2033"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="18">
        <location>
            <begin position="2034"/>
            <end position="2072"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="18">
        <location>
            <begin position="2113"/>
            <end position="2153"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="18">
        <location>
            <begin position="2154"/>
            <end position="2193"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="18">
        <location>
            <begin position="2234"/>
            <end position="2276"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="18">
        <location>
            <begin position="2277"/>
            <end position="2317"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="18">
        <location>
            <begin position="2318"/>
            <end position="2356"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="18">
        <location>
            <begin position="2890"/>
            <end position="2929"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="131"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="500"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="510"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="581"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="805"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="812"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="2900"/>
            <end position="2917"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="2919"/>
            <end position="2928"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAJ8036652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="3151" mass="347213" checksum="DBB2D8135C0BF4A8" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C3N4</accession>
    <name>A0A9Q1C3N4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Meiotic recombination protein DMC1/LIM15 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HOLleu_18519</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Nanhai2018</strain>
            <tissue evidence="15">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Participates in meiotic recombination, specifically in homologous strand assimilation, which is required for the resolution of meiotic double-strand breaks.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Double stacked ring-shaped homooctamer. Interacts with BRCA2. Interacts with the MND1-PSMC3IP heterodimer. Interacts with RAD51AP1; the interaction is direct and stimulates DMC1-mediated homologous recombination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RecA family. DMC1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C3N4"/>
    <dbReference type="OrthoDB" id="10251254at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000794">
        <property type="term" value="C:condensed nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070192">
        <property type="term" value="P:chromosome organization involved in meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000730">
        <property type="term" value="P:DNA recombinase assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042148">
        <property type="term" value="P:DNA strand invasion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006312">
        <property type="term" value="P:mitotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19514">
        <property type="entry name" value="DMC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000239">
        <property type="entry name" value="Meiotic recombination protein DMC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000032">
        <property type="entry name" value="meiotic recombination protein DMC1/LIM15 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011940">
        <property type="entry name" value="Dmc1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016467">
        <property type="entry name" value="DNA_recomb/repair_RecA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010995">
        <property type="entry name" value="DNA_repair_Rad51/TF_NusA_a-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013632">
        <property type="entry name" value="Rad51_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020588">
        <property type="entry name" value="RecA_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020587">
        <property type="entry name" value="RecA_monomer-monomer_interface"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003301">
        <property type="entry name" value="PRK04301.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02238">
        <property type="entry name" value="recomb_DMC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22942:SF30">
        <property type="entry name" value="MEIOTIC RECOMBINATION PROTEIN DMC1_LIM15 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22942">
        <property type="entry name" value="RECA/RAD51/RADA DNA STRAND-PAIRING FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14520">
        <property type="entry name" value="HHH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08423">
        <property type="entry name" value="Rad51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005856">
        <property type="entry name" value="Rad51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47794">
        <property type="entry name" value="Rad51 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50162">
        <property type="entry name" value="RECA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50163">
        <property type="entry name" value="RECA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0469">Meiosis</keyword>
    <keyword evidence="3 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RecA family profile 1" evidence="13">
        <location>
            <begin position="103"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="RecA family profile 2" evidence="14">
        <location>
            <begin position="283"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00056818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00064395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00071838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ8037643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="346" mass="38525" checksum="ACE2C473265AEFF0" modified="2023-09-13" version="1">MERSCSDQVVEMDEEVEEDLDDEESFFQDIDLLQNHGINVADIKKLKSAGICTIKGIQMTTRKKMCDIKGISEAKMEKIKEAAAKLEDHGFVTALNYAVKRKNCFRITTGSTELDKLLGGGIESMAITEAFGEFRTGKTQLSHTLCVTTQLPGANDYPGGKIIFIDTENTFRPDRLRDIADRFNLDHNAMLDNVLYARAYTSEHQLELLDYAAAKFHEEPGVFKLLIIDSIMALFRVDFSGRGELADRQQKLAQMLSKLQKISEEYNLAVFVTNQMTADPGAAMSFQADPKKPIGGHILAHASTTRISLRKGRGENRIAKIYDSPDMPENEATFAISAGGIIDSRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C3Q7</accession>
    <name>A0A9Q1C3Q7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Zinc finger CCHC domain-containing protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_18781</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C3Q7"/>
    <dbReference type="OrthoDB" id="3863715at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002218">
        <property type="term" value="P:activation of innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042509">
        <property type="entry name" value="ZCCHC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22639">
        <property type="entry name" value="GAG-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22639:SF3">
        <property type="entry name" value="ZINC FINGER CCHC DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="102"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="120"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="250"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="326"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="326"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8037855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="528" mass="58797" checksum="62A79166D4368045" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C6F9</accession>
    <name>A0A9Q1C6F9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_16716</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C6F9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040435182" evidence="1">
        <location>
            <begin position="20"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8039098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="185" mass="20771" checksum="E8333E9C72AE0C43" modified="2023-09-13" version="1">MMWSAFAVVLFGLLNSSSSQERKSVKESALTFDVGGKTHHESRRLEDDVVTFTDAEDGFLTVLDYKRGIFMVKNTTTQTCYFSTLENIPYNRDEEKPIFVKEVKEYSELESELGENADVVLFTPAGTLPKSYVRVTNDARVVGRCSSQPSYWLETKSISRKRRAKCEVICGAVILGIGFLAGKWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C7G9</accession>
    <name>A0A9Q1C7G9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_17158</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C7G9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1820">
        <property type="entry name" value="L1 transposable element, RRM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8039439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="118" mass="13617" checksum="C24D0C2C1CEB2E9B" modified="2023-09-13" version="1">MVSTRLELVQACNVDRAHRDGRISQNRSRHILVKVSDFQDKVNILRSARQALSAESFYIIDDLTKINLKEQRKWLSKVNQLYQNGLRLHCQEDVGERKVGSLTVSLMPPKIKTSCYFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C7I1</accession>
    <name>A0A9Q1C7I1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Retrovirus-related Pol polyprotein from type-2 retrotransposable element R2DM</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_13915</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8039802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C7I1"/>
    <dbReference type="OrthoDB" id="2194416at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35450">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35450:SF2">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040410495" evidence="4">
        <location>
            <begin position="22"/>
            <end position="1155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="1123"/>
            <end position="1142"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="23"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="123"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase" evidence="6">
        <location>
            <begin position="286"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="924"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="938"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="56"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="156"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8039802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1155" mass="128447" checksum="F2EF02298BAD9AC4" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C9E8</accession>
    <name>A0A9Q1C9E8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">UPF0489 protein C5orf22</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_11910</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0489 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C9E8"/>
    <dbReference type="OrthoDB" id="418142at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024131">
        <property type="entry name" value="UPF0489"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13225">
        <property type="entry name" value="MISEXPRESSION SUPPRESSOR OF RAS 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13225:SF3">
        <property type="entry name" value="UPF0489 PROTEIN C5ORF22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12640">
        <property type="entry name" value="UPF0489"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="175"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="179"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="195"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="228"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8041171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="469" mass="53811" checksum="C14C8B9BAD0FC1F4" modified="2023-09-13" version="1">MAEIIPRKKRRLFSIIPVVVVEDHHEVLKHIYRALATRHLPFEGICMVHLDSHPDLQIPSKIPAQDVFNLEKLSCHLSIENWIIPPVFAGHINRLVWMKPPWADQLPEGHRRVVVGEHKTHKTIRINWPDEYYLSDVMYAAEDDMINCKQLDVNIITVKYRTNHEYSKFQTQEQSNRLSYSSEQCEQELQSSSPKTKDSVHSFRNDPVSDKVDTIDKDQAADSKSLLPDTNSKNENEGDNLNSDVVSVIQEAVSCSEAFILDIDLDFFSTQNPFKLEYTPEQYAVLKELYHFEAPKDGSREEVDRSQSKRDKQLKDLHRALAWCLNEKTDNVGNTEKLSDRWLKMQGLIEDLKASSKETLNGELIHMAGETTCDDEDDLPHHVSTEEEIAALVKQVKVILSMQPKPIMVTIARSSEDDYCPKGQVNKIQELVVTMLKELYGEIEIKLDYELKSDVSQKESSNESNIPTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CAB3</accession>
    <name>A0A9Q1CAB3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_12044</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CAB3"/>
    <dbReference type="OrthoDB" id="410381at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8041272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="50" mass="5460" checksum="983BB601F55CB039" modified="2023-09-13" version="1">MSCGKAPGTDAIPAEIYKEGGPTVIQRLTQLFQVIWNDAQLPQQFKDAAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CAM6</accession>
    <name>A0A9Q1CAM6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Phenylalanine--tRNA ligase, mitochondrial</fullName>
            <ecNumber evidence="4">6.1.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Phenylalanyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">HOLleu_11975</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">Nanhai2018</strain>
            <tissue evidence="19">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Is responsible for the charging of tRNA(Phe) with phenylalanine in mitochondrial translation. To a lesser extent, also catalyzes direct attachment of m-Tyr (an oxidized version of Phe) to tRNA(Phe), thereby opening the way for delivery of the misacylated tRNA to the ribosome and incorporation of ROS-damaged amino acid into proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>tRNA(Phe) + L-phenylalanine + ATP = L-phenylalanyl-tRNA(Phe) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19413"/>
            <dbReference type="Rhea" id="RHEA-COMP:9668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78531"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.20"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.20" evidence="4"/>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CAM6"/>
    <dbReference type="OrthoDB" id="4457at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006432">
        <property type="term" value="P:phenylalanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00496">
        <property type="entry name" value="PheRS_alpha_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.380:FF:000002">
        <property type="entry name" value="phenylalanine--tRNA ligase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000041">
        <property type="entry name" value="Phenylalanyl-tRNA synthetase 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.380">
        <property type="entry name" value="Ferrodoxin-fold anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005121">
        <property type="entry name" value="Fdx_antiC-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036690">
        <property type="entry name" value="Fdx_antiC-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004530">
        <property type="entry name" value="Phe-tRNA-synth_IIc_mito"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002319">
        <property type="entry name" value="Phenylalanyl-tRNA_Synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00469">
        <property type="entry name" value="pheS_mito"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538:SF41">
        <property type="entry name" value="PHENYLALANINE--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538">
        <property type="entry name" value="PHENYLALANYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03147">
        <property type="entry name" value="FDX-ACB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01409">
        <property type="entry name" value="tRNA-synt_2d"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00896">
        <property type="entry name" value="FDX-ACB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54991">
        <property type="entry name" value="Anticodon-binding domain of PheRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51447">
        <property type="entry name" value="FDX_ACB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="12" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 19" id="KW-0436">Ligase</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="17">
        <location>
            <begin position="96"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="FDX-ACB" evidence="18">
        <location>
            <begin position="364"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00060211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00073229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAJ8041226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="458" mass="53497" checksum="F4FB29BEF94B9E9C" modified="2023-09-13" version="1">MKFLNILKRLPKQVDQVRAYHCLPWPWVHHHKSKTIQSCILGGRHQRLYSQINQDSDILVSGGSFSRDGFTTVTPKILSRVGVNLHNERNHPLCIIKEQIRDFFYKRFLNKRGNPLFSIYDNLSPVVTVWQNFDSVLVPKDHVSRRRGDNYYLNREYMLRAHTSAHQHELVKVGLDAFLVAGDVYRRDEIDATHFPVFHQMEGVQLFSDFELFEKCQDSSLKLFTDDQERVPEKQEHHTLEAVKLVEFELKHTLEDLARFLFGEGIETRWVVTDFPFTYPSFELEIKFQGEWLEVLGCGIMEQELLHSAGAGHKIGYAFGLGLERLAMVLFHIPDIRLFWSTDSRFRSQFEVPDPRKVKFKPFSKFPPVFYDISFWCPESGFISNDFYDLVRSVAGEMAEKVELIDEFCNPKTGKTSHCYRITYRSMDRTLTKEEVGTIHTELIEQCSTSLGVEVRNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CAY2</accession>
    <name>A0A9Q1CAY2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Aquaporin-10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_12216</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the MIP/aquaporin (TC 1.A.8) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CAY2"/>
    <dbReference type="OrthoDB" id="3222at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015254">
        <property type="term" value="F:glycerol channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015204">
        <property type="term" value="F:urea transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015250">
        <property type="term" value="F:water channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00333">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000425">
        <property type="entry name" value="MIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050363">
        <property type="entry name" value="MIP/Aquaporin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022357">
        <property type="entry name" value="MIP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43829">
        <property type="entry name" value="AQUAPORIN OR AQUAGLYCEROPORIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43829:SF9">
        <property type="entry name" value="AQUAPORIN-9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00783">
        <property type="entry name" value="MINTRINSICP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81338">
        <property type="entry name" value="Aquaporin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00221">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="58"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="103"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="158"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="192"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="244"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8041408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="309" mass="33618" checksum="58D2813F5CB36971" modified="2023-09-13" version="1">MPTEQILKNLQTKIRVKNDLLRCLLSEFFATLLFVLIVNGSVASVITTGLDPVEHHFWISWAVGMGAGLGIWTGYGVSGGHVNPSVTIGLASTGKFPWRRVPIYFLTQFLAAMVACVICFLCYYDAINAFDGGERQAVGHENATCGIFTSFPGPHLSIGVGFFEQIVNTGILLALILMVADSRNAGPPFNVSPFYYGFTVLVVLLSYGVNAGAPLNPARDLAGRVMCAIAGYGPDVWVPYGKHWWWIGTFGPLVGAVTGSLLYYIMIEIHHPPEEDSEKDNHNESGYQIIAKSAEMVESHDKVNTETGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CAY4</accession>
    <name>A0A9Q1CAY4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_12490</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CAY4"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="31"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8041620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="96" mass="10668" checksum="9BA0447AE181E163" modified="2023-09-13" version="1">MLAVNTTTDAAVTATPTSDPRTRGGGITHIVYMYITCFGIISIVLILTIILRCIRKLQKKAKLAKPTIYRNRSTQGQQLVTQELDEFQSSDKARLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CBC5</accession>
    <name>A0A9Q1CBC5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Interferon-induced very large GTPase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_13646</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class dynamin-like GTPase superfamily. Very large inducible GTPase (VLIG) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8042558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1597724at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030383">
        <property type="entry name" value="G_VLIG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057365">
        <property type="entry name" value="URGCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052986">
        <property type="entry name" value="VLIG_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14819:SF25">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_52, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14819">
        <property type="entry name" value="GTP-BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25496">
        <property type="entry name" value="URGCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25683">
        <property type="entry name" value="URGCP_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51717">
        <property type="entry name" value="G_VLIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VLIG-type G" evidence="3">
        <location>
            <begin position="723"/>
            <end position="965"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="513"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="599"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1230"/>
            <end position="1260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1623"/>
            <end position="1654"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1764"/>
            <end position="1792"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="606"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1230"/>
            <end position="1239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1626"/>
            <end position="1636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1772"/>
            <end position="1792"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8042558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1792" mass="205096" checksum="A342358C174FDB63" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CC48</accession>
    <name>A0A9Q1CC48_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ectonucleotide pyrophosphatase/phosphodiesterase family member 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_12776</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CC48"/>
    <dbReference type="OrthoDB" id="415411at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd16018">
        <property type="entry name" value="Enpp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002591">
        <property type="entry name" value="Phosphodiest/P_Trfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10151:SF120">
        <property type="entry name" value="BIS(5'-ADENOSYL)-TRIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10151">
        <property type="entry name" value="ECTONUCLEOTIDE PYROPHOSPHATASE/PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01663">
        <property type="entry name" value="Phosphodiest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040381936" evidence="2">
        <location>
            <begin position="27"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="433"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8041854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="496" mass="56574" checksum="3149F42BC62F92C3" modified="2023-09-13" version="1">MQSFMPSLYIWLSSALLYSLLKLSASKPLSQSREKLVLLISFDGFRWDYLNRGLTPTFNKFASDGGKANYTIDAFITKTFPNHYTLVTGLYEESHGIVANDIYDPILHERFSLGNLDPKWWSQNGQEPIWITNQRQGGSSGVVDYPGMDVKFDGMLPNFEYDAYNETVPFNERIDFTVELFSNESINFAALYFHEPDRTGHLFGPDSKEMNETLKLCDDTMNYLIQKLKDADLYDKINIIVTSDHGMASTDLSQTIFAVDYLNGTVYPYFTTDNNPVLGIWPYNTSHVDPLYQAMKKAPHISVYKKDEIPKEYHYRNNVRIPPIVAVADEGWMLRNLMVNFSNLNPVKGDHGYNNSLMSMHPFFLVHGPAFHPGSICPPFSSVDVYPLMCHLLGIKPRPNNGTLKIVQQLLKEFKKGPRGTPDTGIDGRHNDYFTVGIAFACVLVMLAIVLVTGMCLRQRKYRQTQRIYHTLDDGIDSEDEEIFEDHDKMLPSTNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CC77</accession>
    <name>A0A9Q1CC77_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Adhesion G-protein coupled receptor G4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_09881</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8042978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CC77"/>
    <dbReference type="OrthoDB" id="10037534at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22827">
        <property type="entry name" value="Gal_Rha_Lectin_SUL-I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.220.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053066">
        <property type="entry name" value="ADGR_G7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057244">
        <property type="entry name" value="GAIN_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046338">
        <property type="entry name" value="GAIN_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000203">
        <property type="entry name" value="GPS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000922">
        <property type="entry name" value="Lectin_gal-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043159">
        <property type="entry name" value="Lectin_gal-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47767">
        <property type="entry name" value="ADHESION G PROTEIN-COUPLED RECEPTOR G7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47767:SF2">
        <property type="entry name" value="GPS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01825">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02140">
        <property type="entry name" value="SUEL_Lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00249">
        <property type="entry name" value="GPCRSECRETIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00303">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50221">
        <property type="entry name" value="GAIN_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50228">
        <property type="entry name" value="SUEL_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="514"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="552"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="583"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="620"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="663"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="713"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="737"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="domain" description="SUEL-type lectin" evidence="8">
        <location>
            <begin position="49"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="GAIN-B" evidence="7">
        <location>
            <begin position="315"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="9">
        <location>
            <begin position="516"/>
            <end position="762"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8042978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="830" mass="92938" checksum="33F2C49D6CDE5A5E" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CCB5</accession>
    <name>A0A9Q1CCB5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">DC-STAMP domain-containing protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_09552</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8042716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="5985669at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051856">
        <property type="entry name" value="CSR-E3_Ligase_Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012858">
        <property type="entry name" value="DC_STAMP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21041">
        <property type="entry name" value="DENDRITIC CELL-SPECIFIC TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21041:SF9">
        <property type="entry name" value="DENDRITIC CELL-SPECIFIC TRANSMEMBRANE PROTEIN-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07782">
        <property type="entry name" value="DC_STAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26039">
        <property type="entry name" value="Dcst2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="630"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="660"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="688"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="728"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1037"/>
            <end position="1058"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="1134"/>
            <end position="1155"/>
        </location>
    </feature>
    <feature type="domain" description="Dendritic cell-specific transmembrane protein-like" evidence="7">
        <location>
            <begin position="987"/>
            <end position="1178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="233"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="359"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="397"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="448"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="607"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="24"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="90"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="157"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="244"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="359"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="419"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="450"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="462"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="480"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="513"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="523"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="542"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="607"/>
            <end position="617"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8042716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1290" mass="143988" checksum="ADB61673F8C126D2" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CD37</accession>
    <name>A0A9Q1CD37_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Echinoidin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_13572</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8042503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CD37"/>
    <dbReference type="OrthoDB" id="7357196at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050111">
        <property type="entry name" value="C-type_lectin/snaclec_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22803">
        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C-type lectin" evidence="1">
        <location>
            <begin position="30"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8042503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="171" mass="19299" checksum="F8E87167D52D09D1" modified="2023-09-13" version="1">MGKVLHRSVCRDGHFGAGTVTVFEMSNFCYCRYFNNLRLDWIAAEKYCNQFGTVHEGAEDQLAHLVSIHSEHENTFVRALWESLREPSDNRQWCWIGYNDLATEGEFVWSDQSPPQFSKWGTGQPNSHGGDQDCGAIDTSGHGPNLGSWDDGTCSSRLPFMCKMLPVHLSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CD61</accession>
    <name>A0A9Q1CD61_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">exodeoxyribonuclease III</fullName>
            <ecNumber evidence="3">3.1.11.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_09631</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.</text>
            <dbReference type="EC" id="3.1.11.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="7">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="7">Probably binds two magnesium or manganese ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA repair enzymes AP/ExoA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.11.2" evidence="3"/>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8042775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CD61"/>
    <dbReference type="OrthoDB" id="416119at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003906">
        <property type="term" value="F:DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008311">
        <property type="term" value="F:double-stranded DNA 3'-5' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09076">
        <property type="entry name" value="L1-EN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004808">
        <property type="entry name" value="AP_endonuc_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748">
        <property type="entry name" value="AP ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748:SF26">
        <property type="entry name" value="ENDONUCLEASE_EXONUCLEASE_PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0464">Manganese</keyword>
    <keyword evidence="4 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="9">
        <location>
            <begin position="5"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="8">
        <location>
            <position position="145"/>
        </location>
    </feature>
    <feature type="site" description="Important for catalytic activity" evidence="8">
        <location>
            <position position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR604808-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR604808-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8042775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="368" mass="42817" checksum="E3E9E6902C35BD7B" modified="2023-09-13" version="1">MIKFCTFNVNGLKDDLKRRRVLQFFKLFQFDIIFLQEFHFNCNVDVSKFQREWGRRVFVSTSPSAHFSGVGILFNPKFACTVSQCQRDREGRLISMLIHHAKSNSKIRLCNVYAPNVPSQRRVFFQNLSNFVHGPEPTLLAGDFNCIFHNDDRSGVSANTSCFIGREELQSFLSARSLVDAWVESSPHSPGHTWFHSGKGQSSRLDRIYVPPPLTRLIFLYIVFRFLTTTPWLCILPLKIFSQGKGVWKCSVSILEDTDFCNDFRIHYPLWSTLKPGYNSIVDWWEDCKEREKHSMIRHGVRLARERRDRLAELESKCISAGGAELAQILDDQRRGEPRFRSRERYLEHGEIEAFRFFLSSGKTLCRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CDW2</accession>
    <name>A0A9Q1CDW2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transmembrane protein 120A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_10904</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM120 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CDW2"/>
    <dbReference type="OrthoDB" id="2015098at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012926">
        <property type="entry name" value="TMEM120A/B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21433:SF0">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 120 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21433">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN INDUCED BY TUMOR NECROSIS FACTOR ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07851">
        <property type="entry name" value="TMEM120A-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="133"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="160"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="190"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="220"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="272"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="306"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8043698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="341" mass="40304" checksum="9AEFF8D125742FD9" modified="2023-09-13" version="1">MDPSSQVSLQEEFDDLGEEYETLVKQHEVYRTKLAELKKIQSKCLSLLAHQNKKYKQLQASFKGSVSGSSAEVREEDTVLQKKIQERQKSCKTMQESLPKQNSFLLKVIIGQISVSLLTREERYDYKEGYERFKLYTTIIMLIYSLVNLFIVQYRFIDAIYHFMIVWYYCTLVLRENILRHNGSRIKGWWVIHHYLTVLLSGILLIWPDSISYRMFRTQFMVFGVYLNFLQLLQYYYQSGCLYRLRALGEKSDLDVTIAEEFQTWMWRGLTFLLPFLFFGYLFQLYNAITLYMISRHPDCHEWMVLSSAIIFFVLFSGNSATLSKVVIDKLSKSKPKSKTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CE26</accession>
    <name>A0A9Q1CE26_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_10817</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CE26"/>
    <dbReference type="OrthoDB" id="7975104at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF7">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8043631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="130" mass="14629" checksum="7F80A051F813A3F2" modified="2023-09-13" version="1">MKIMYKPGRETLLDDAMSQLNLIPCRSKGKLIKLKQAINEDPKMTALSEHIINGWPEKQRSLPQPLLQYLSCRDELTGEEGGVIMKVQRILIPQCGKGILAKLHDAHQSIVKCQLRAKSTVFLVKNQPGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CGC8</accession>
    <name>A0A9Q1CGC8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Elongation factor Tu</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HOLleu_11315</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Nanhai2018</strain>
            <tissue evidence="17">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CGC8"/>
    <dbReference type="OrthoDB" id="2067at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070125">
        <property type="term" value="P:mitochondrial translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01884">
        <property type="entry name" value="EF_Tu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03697">
        <property type="entry name" value="EFTU_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03706">
        <property type="entry name" value="mtEFTU_III"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Elongation factor Tu"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Elongation factor Tu"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Elongation factor Tu"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041709">
        <property type="entry name" value="EF-Tu_GTP-bd"/>
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    <dbReference type="InterPro" id="IPR050055">
        <property type="entry name" value="EF-Tu_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033720">
        <property type="entry name" value="EFTU_2"/>
    </dbReference>
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        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
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        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004541">
        <property type="entry name" value="Transl_elong_EFTu/EF1A_bac/org"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004160">
        <property type="entry name" value="Transl_elong_EFTu/EF1A_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00485">
        <property type="entry name" value="EF-Tu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000766">
        <property type="entry name" value="PRK00049.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009372">
        <property type="entry name" value="PRK12735.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009373">
        <property type="entry name" value="PRK12736.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43721:SF36">
        <property type="entry name" value="ELONGATION FACTOR TU, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43721">
        <property type="entry name" value="ELONGATION FACTOR TU-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03143">
        <property type="entry name" value="GTP_EFTU_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 15 17" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="13 15" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Tr-type G" evidence="16">
        <location>
            <begin position="64"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00051990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ8043984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="457" mass="50387" checksum="4F43A5F44F838B56" modified="2023-09-13" version="1">MASIANLRLCIFSAGLRKLNFPQTFKDVSHLQRQTLLQTQLCSLKTSAAVLAEPKGKTSFKRTKPHLNIGTIGHVDHGKTTLTAAITKVLSEAGGAKFHKYDEIDKAPEEKKRGITINAAHVEYETSNRHYAHTDCPGHADYIKNMITGTAQMEGAILVVAATDGQMPQTREHLLLAKQIGVDKIVVFINKADAVDDEVLELVELEMREVLAEFGFDGENTPIIIGSALQALEGKEGKIGIDCVHELLGAVDEWIPLPVRDLDKEYMMPIEASYTIQGRGTVVSGRVERGKIKKNEEVELVGHGRKIKTVVTGLEMFHKMLEEGQAGDQMGALLRNVKRDDTKRGMVMCKPGSLDLHNHVEAQVYILGKDEGGRHKPFTSNFSPQMYSYTWDVAAKIQLIGGKEMVMPGEDATLNLQLLKPMVCEQGQRFTLRDGKITLGTGVITKVLPNIEEEDGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CHD7</accession>
    <name>A0A9Q1CHD7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">StAR-related lipid transfer protein 9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_07633</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CHD7"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002913">
        <property type="entry name" value="START_lipid-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47117:SF8">
        <property type="entry name" value="KINESIN FAMILY MEMBER 16B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47117">
        <property type="entry name" value="STAR-RELATED LIPID TRANSFER PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01852">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50848">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="START" evidence="2">
        <location>
            <begin position="454"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="352"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="375"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="392"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8044783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="641" mass="71925" checksum="501CFC2F4C7A432E" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CIU4</accession>
    <name>A0A9Q1CIU4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">BET1-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_04862</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endomembrane system</location>
            <topology evidence="8">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CIU4"/>
    <dbReference type="OrthoDB" id="261831at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15853">
        <property type="entry name" value="SNARE_Bet1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039899">
        <property type="entry name" value="BET1_SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12791">
        <property type="entry name" value="GOLGI SNARE BET1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="97"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="11">
        <location>
            <begin position="24"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8046247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="123" mass="13702" checksum="4AB622929E64B710" modified="2023-09-13" version="1">MADSWERGNRRNGGSWGGAAGTEDMLEAENNRLQDGLAGKVSQIKMLAMDIDNEAKEQNRYLDGMQGGDFESNVGLLGGTIKRFDTMLSSGRGNRRLMCYLIGIVVIIFFFAYFLIERVTKHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CJF0</accession>
    <name>A0A9Q1CJF0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Plexin-A4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_05136</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CJF0"/>
    <dbReference type="OrthoDB" id="125363at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002116">
        <property type="term" value="C:semaphorin receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017154">
        <property type="term" value="F:semaphorin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007162">
        <property type="term" value="P:negative regulation of cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050772">
        <property type="term" value="P:positive regulation of axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030334">
        <property type="term" value="P:regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031148">
        <property type="entry name" value="Plexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016201">
        <property type="entry name" value="PSI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041019">
        <property type="entry name" value="TIG1_plexin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22625">
        <property type="entry name" value="PLEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22625:SF44">
        <property type="entry name" value="PLEXIN-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24479">
        <property type="entry name" value="PSI_PlexinA-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17960">
        <property type="entry name" value="TIG_plexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00423">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PSI" evidence="2">
        <location>
            <begin position="161"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8046462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="380" mass="42424" checksum="744CD720A809B8C9" modified="2023-09-13" version="1">MLYLVITRAYDELDIVGISAISLNGECAAQSYLSHPDVYGQKQVTLLVLLDLSAAFDTIDHSILEIVLETYYGITGDVLAWFQLRITHLPELKDGEYYECHFNDWETEATVSGDHLNCTSPPENKVPAVPNGGADHVTMQLQIYSSETDENFVTRAYEFYDCSRIRSCSDCVTSKWKCDWCVLKHMCTHDSSSCDRVIFETYASPSNSSSDCPQIIPGEKKLVPSGVDQVISFQAKHLPPLSTSNGVYSYMKPQQTLDAKLILKWKTENVVDNFQPVAKWLEHIELLPEGLGFKSWPGHTKDLKMESTAFVLGAQHTQWSRKNKPVPSVPVASPRGMGQEQGLPKTFDLPNEIVVQPFLCHPVPQVPSWGSAVKSCYAPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CK38</accession>
    <name>A0A9Q1CK38_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_05463</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CK38"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF13">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="146"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="146"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="161"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="185"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8046698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="318" mass="36175" checksum="F811AC7E082A0AC4" modified="2023-09-13" version="1">MRKDEIKRYVLEYLVDESILPSTVLETLITVPTDNTFELKRLEMEMNKEEDNVEKFFNHFEKLGEQLKWPRDKWSILIQSNFTGKAQEVYSALSIEDSMDYDKEFKRCVNDDIKTYLEENKADKLSEVANLADQYALTHKFSKVGQTQNKGQCSRPNKKYGNSSGTPGNSGSQSGSICPTNSGKKQPDSSRQKNDQVSSNKAAFKDIICGFCHKKGHTIANCFQLAKLKESETASNVLANVENSPEVVVTLRPNSNEQIKEEYLPFVSEGFISLDGNSAHPPVKIKILQLCVTQAQLSRCFLMEFCHYRTLLPHEQMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CKJ3</accession>
    <name>A0A9Q1CKJ3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_04871</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CKJ3"/>
    <dbReference type="OrthoDB" id="8816800at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028002">
        <property type="entry name" value="Myb_DNA-bind_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21411">
        <property type="entry name" value="APONTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21411:SF0">
        <property type="entry name" value="REGULATORY PROTEIN ZESTE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13873">
        <property type="entry name" value="Myb_DNA-bind_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb-like" evidence="3">
        <location>
            <begin position="43"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="302"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8046254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="398" mass="44738" checksum="FE210DDF6527CA36" modified="2023-09-13" version="1">MTDEVVVKMEQRPSSAGSNEAGSLGPGKGKSTGTPVRTVKLRNVRLRKPNWTLRESQDLLDEFDKHKLILNARGNTGVSTKAKRKLAWTRIAHKINSKDYSAVKRTVYECKRRWKNMVTFAKMSILEYIKAIKLGGEPPKTVSAMDRKITEVHGDVKLLHTLRSAERRELDDLTDEVGIDSAREITGGNKGAGCTPTSSYENPTLHLTLVEPLSRSQQTAQTSRHFYSDGFVMETDNGQQEEATYQSFEEQTPSIENYYSLQSSNPDSYHGASGIALSQGAEHSFVDEPRYARGLSDENVDTQEVILRRRKLQMEIEKLQLEKEVLEMQKAKIRLETKAWRKFVSQRSSFQTASPFSMPPFYWPSPAPGFPAFYGSSPNIGHSGVTQLHANSAIKKHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CL96</accession>
    <name>A0A9Q1CL96_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Dual specificity protein phosphatase 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_06384</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CL96"/>
    <dbReference type="OrthoDB" id="165342at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033550">
        <property type="term" value="F:MAP kinase tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017017">
        <property type="term" value="F:MAP kinase tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008330">
        <property type="term" value="F:protein tyrosine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01446">
        <property type="entry name" value="DSP_MapKP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14566">
        <property type="entry name" value="DSP_MKP_classII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000004">
        <property type="entry name" value="Protein phosphatase Slingshot homolog 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008343">
        <property type="entry name" value="MKP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159:SF519">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE MPK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01764">
        <property type="entry name" value="MAPKPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="8">
        <location>
            <begin position="35"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="6">
        <location>
            <begin position="193"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="7">
        <location>
            <begin position="256"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="347"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="369"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8047396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="392" mass="43742" checksum="35E7732AC5C89D4E" modified="2023-09-13" version="1">MPAQTTVNNNCSDIDSMAMSRSPDMVAELLGSGGNEPGVLLLDFRPQTAFSKGHVRHALKVTIPKILLKRLENGSLKSSSFKSLINGDEGKEIFFKHSSTASIILYDAKSRNCDINANSDSSFVLLLRRLKENGFRVGYLDGGFEAFQMNYPNLCVTDTSSDSSEDTLPDLGFDKLRIEHSPEDKHEYQVENFPVEILPYLYLGSKRECENLELLRQYQITYVLNVTPNIPNHFENDGLKYLQIPIRDRWNQNLAEHFPEAIDFIDEARSSKSGILVHCHGGVSRSVTVTVAYLMQKQGLSLNDAYDFVKQRKANIAPNVNFLDQLQDFESELASSPCRSGRCSCSSVQGKKCMSPDPTFLSSSASSSERTTPLSTNTSISSAESKREFDFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CLG5</accession>
    <name>A0A9Q1CLG5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_05469</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CLG5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8046704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="67" mass="7459" checksum="2AA57622CE2A518D" modified="2023-09-13" version="1">MPIMIQFQRKIVLIKTVKAEDGSLAHVIAQCNLNGTYGLMSDRNQGSIDWEHLSGGNTGLKRTEMKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CNN5</accession>
    <name>A0A9Q1CNN5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_06955</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CNN5"/>
    <dbReference type="OrthoDB" id="10068084at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8047844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="447" mass="50082" checksum="FAD664533CE810B8" modified="2023-09-13" version="1">MSRYLYSEKLEEKLDQGSEVLTEVKAKLRGLNLDTVGSSTQEPTPQVQTAATVSSSRKTGENNNWGNLPWKKDFKIAGQIGEPGQKDRLSFASLARQIEAGVKKGFSHDDVTEAVIKAIVPGSRLRNYLEGRTTISLPELRSLLRAHFQEKEATTLFQELSTAFQGPKETAHSFVLRTLDLRQKVVFASQEAGAGVIYTYEQAQKMFLHTVSTGLQSDYVRQQLQPLLTQEATTDEALLEALTVFVGQEAERAMKLKKSKVHTTALWDTGAQVSLVSEEWVKQHLPQTLVRPMEDLLDSEGLRLNTATGTPIGFQGWIEVKFQIPNATLTGDEIIVPMLVTEEILETPIIGYNVIEEMAQRGSMKSTQKQFCKLLPQMSYPQVNKLVNMLQTVDSEEMSTVKTKRNSVKVMAGETIIVRCTTHRRAHEEVTTLFEPDENHQLSFQRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CPA1</accession>
    <name>A0A9Q1CPA1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Hemicentin-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_00929</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CPA1"/>
    <dbReference type="OrthoDB" id="6159398at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040145027" evidence="1">
        <location>
            <begin position="29"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="116"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8048565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="226" mass="25724" checksum="AE635FDF29D93362" modified="2023-09-13" version="1">MRVNKPKVIFSSLMVLFLVVVLFPLNESQQSWTVAISSSVLFPCNSDPYKMRIWEHDNNVLFNNKLKIDFKLDHIYLLDNYSLLIQEITLPHKGIYRCLQDGSIAEEFLLNVEVPPKMFLTINEFDSVMKISVEEGTDITLFCHAFGALPPVNLSWAIDGEENDYRALNFTVAVGDDYQGRQVFNSVSSYTFKLIRNNGTVSCRSNTVSGVGKREINLQYSTFGKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CTQ3</accession>
    <name>A0A9Q1CTQ3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_04157</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CTQ3"/>
    <dbReference type="OrthoDB" id="10056483at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15489">
        <property type="entry name" value="PHD_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020847">
        <property type="entry name" value="AP_endonuclease_F1_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46364">
        <property type="entry name" value="OS08G0421900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00726">
        <property type="entry name" value="AP_NUCLEASE_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="5">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="5">
        <location>
            <begin position="52"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8050813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="215" mass="23794" checksum="CA8047A7D676DB60" modified="2023-09-13" version="1">MCAKLVKKNDNAVQCDGCDHWVHIRCADIDREGYKLLQLSKPEDKWYCSKCSSPCGICLKGVRGDDPAIECDSCKKWIHNRCSLVDDSHYDHIQATNCIWMCPNCDNGNLSNTWGLTAPQTENSFHALNPDSGNAHSIPSIDGKSSARRPSGKTALKKEVSIVSININGLRGKKLDLQTILSESNPDVVALQETKIDSTVTNTELIPPCLEYEIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CTU3</accession>
    <name>A0A9Q1CTU3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_04417</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8051010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CTU3"/>
    <dbReference type="OrthoDB" id="6776853at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8051010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="634" mass="72364" checksum="B4B7BB0CA4EBAB5F" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1GZN3</accession>
    <name>A0A9Q1GZN3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">b(0,+)-type amino acid transporter 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Glycoprotein-associated amino acid transporter b0,+AT1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Solute carrier family 7 member 9</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">HOLleu_30969</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">Nanhai2018</strain>
            <tissue evidence="20">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-phenylalanine(out) + L-arginine(in) = L-phenylalanine(in) + L-arginine(out)</text>
            <dbReference type="Rhea" id="RHEA:71067"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:71068"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-cysteine(out) + L-arginine(in) = L-cysteine(in) + L-arginine(out)</text>
            <dbReference type="Rhea" id="RHEA:71071"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:71072"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-cystine(out) + L-arginine(in) = L-cystine(in) + L-arginine(out)</text>
            <dbReference type="Rhea" id="RHEA:71075"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:35491"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:71076"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-histidine(out) + L-arginine(in) = L-histidine(in) + L-arginine(out)</text>
            <dbReference type="Rhea" id="RHEA:71063"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:71064"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-leucine(out) + L-arginine(in) = L-leucine(in) + L-arginine(out)</text>
            <dbReference type="Rhea" id="RHEA:71059"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:71060"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-lysine(out) + L-arginine(in) = L-lysine(in) + L-arginine(out)</text>
            <dbReference type="Rhea" id="RHEA:70827"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:70828"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1GZN3"/>
    <dbReference type="OrthoDB" id="5982228at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000015">
        <property type="entry name" value="B(0,+)-type amino acid transporter 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050598">
        <property type="entry name" value="AminoAcid_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785:SF512">
        <property type="entry name" value="SOBREMESA, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="56"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="99"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="149"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="178"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="252"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="301"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="346"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="373"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="407"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="434"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00051323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00051814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00051835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00052179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00052732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00074336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00079910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00083296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00093193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAJ8028664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="496" mass="54362" checksum="B6CD5A4EA01980A4" modified="2023-09-13" version="1">MPESSETGSTGDVTLKRQVGLVSCVALIVGTIIGSGIFISPVYVLKETQSVGMSLVIWFLGGTLAMIGALCFSELGTMIPKSGGTYIYVLECMGPIPAFLFSWTCALILMPASVSAIALSFGYYMAEPLYPSHDCQDETKKKFDMVSKLFACLCILLLTFINCVSVKAATYVQNSLTVAKLIALAIIIGVGFIRICQGYTEHLNPSVSFEGSATNLFSYGIAFYQSLWAYDGWNQLNFVTEEIKNPYRNLPYGILIGIPLVTLVYLFTNMAYFTVLTPDEMLNSAAVAVTFANRTLGPMAWIMPLLVCCSTVGAVNGCLFTAGRLPFVTAREGHMMDILAMVHVKYFTPLPSIVLTGAIAILMLLPADFSALVNYFSFAAWLFYGLTILALIILRVQRPNAKRPIKIPIIIPIVGLVASIYLVIAPIIDEPSLEIFYALLFILSGLLVYFPFVHFGLRIPYVMRPTQIFLQKLMFVAPSSYSSSVEQLGKQLAEKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H2E9</accession>
    <name>A0A9Q1H2E9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Sorting nexin-24</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_29635</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sorting nexin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8030060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H2E9"/>
    <dbReference type="OrthoDB" id="93876at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901981">
        <property type="term" value="F:phosphatidylinositol phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052467">
        <property type="entry name" value="Sorting_nexin_PX-domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15813:SF9">
        <property type="entry name" value="PX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15813">
        <property type="entry name" value="SORTING NEXIN-22 AND 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="5" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PX" evidence="8">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8030060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="172" mass="19484" checksum="2B0F9E857C62B698" modified="2023-09-13" version="1">MISISIPSFRQVVPEDERPYTAYQIHVKVAGRTHVIEKRYREFHSFHKKLRKTIDIPDFPPKKVLQLSAKGLEHRRQGLENYFNAIVTTGTIPKSLLTFLKVNNLNKAPSFDSLDDLNTDSGSTSTHQPVLIYENDPYLNPPSGKPDILVEGVLHGLYDHYPVEPENSSSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H2R4</accession>
    <name>A0A9Q1H2R4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">THAP domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_26785</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H2R4"/>
    <dbReference type="OrthoDB" id="7683421at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006612">
        <property type="entry name" value="THAP_Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31751:SF42">
        <property type="entry name" value="PROTEIN CBG10204"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31751">
        <property type="entry name" value="SI:CH211-108C17.2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05485">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00692">
        <property type="entry name" value="DM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00980">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50950">
        <property type="entry name" value="ZF_THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="THAP-type" evidence="7">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="251"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="293"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="862"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="259"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="311"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="351"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="883"/>
            <end position="897"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8030375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="912" mass="103155" checksum="6F1FF6A71DE61EF9" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H347</accession>
    <name>A0A9Q1H347_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Alpha-1A adrenergic receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_26886</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H347"/>
    <dbReference type="OrthoDB" id="10037617at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00637">
        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45698:SF1">
        <property type="entry name" value="TRACE AMINE-ASSOCIATED RECEPTOR 13C-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45698">
        <property type="entry name" value="TRACE AMINE-ASSOCIATED RECEPTOR 19N-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 5 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="30"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="63"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="106"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="148"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="187"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="290"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="327"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="8">
        <location>
            <begin position="43"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="234"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="266"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="234"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8030451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="384" mass="43640" checksum="08CC42F8949D1FBE" modified="2023-09-13" version="1">MENTTMQNLTTILSPVDEISDLSFSDILTVFIYTVIGVAGIIGNMLVLIIFRHLKSRKSQVNSFILNQASADLMTSIFLISFGATLLARDSIFHVGFGGEFMCRFWWSRFFIFSCFAVSTFNLTSMSIERFIAVVLPLRYPLIFTKRFTLSVILFIWIVAPLMQWIFPLALYELDHVTASCRFQSSWSFAVAGAISGVTLFLWEYFIPVIIMLVAYISILRTLKRKETQIQSFGQSANTQSTSQPPAPNNDDTGNRSSGIRFKVDNGNQATADRQPPKSKSSSAQARRRNVTITLFIMFIMYFICWTPNQLTFLRFNLGGPLDFNGFWYHFTVFMAFVNTCINPFIYALKHKQFQKGIKDFLGCALKKEDRSSQINSVSSTVVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H351</accession>
    <name>A0A9Q1H351_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">dTDP-D-glucose 4,6-dehydratase</fullName>
            <ecNumber evidence="4">4.2.1.46</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_27934</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>dTDP-alpha-D-glucose = dTDP-4-dehydro-6-deoxy-alpha-D-glucose + H2O</text>
            <dbReference type="Rhea" id="RHEA:17221"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57477"/>
            <dbReference type="ChEBI" id="CHEBI:57649"/>
            <dbReference type="EC" id="4.2.1.46"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NAD(P)-dependent epimerase/dehydratase family. dTDP-glucose dehydratase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.46" evidence="4"/>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8031258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H351"/>
    <dbReference type="OrthoDB" id="16464at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008460">
        <property type="term" value="F:dTDP-glucose 4,6-dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009225">
        <property type="term" value="P:nucleotide-sugar metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05246">
        <property type="entry name" value="dTDP_GD_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000304">
        <property type="entry name" value="UDP-glucose 4,6-dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005888">
        <property type="entry name" value="dTDP_Gluc_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43000">
        <property type="entry name" value="DTDP-D-GLUCOSE 4,6-DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16363">
        <property type="entry name" value="GDP_Man_Dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-binding" evidence="8">
        <location>
            <begin position="27"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00067702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF16363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8031258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="360" mass="40831" checksum="5563064FB6F69F10" modified="2023-09-13" version="1">MAGNGQIVVENGHTTPSNGYHRRILFSGGSGFIGSHVVDKLVLHPHIQYYVVNYDKLDYCASLKNNSLVEGKSNYKFVKGDINDKLKLLQVMKNEKCDTVMHFAAQSHVDNSFRNDKPFHEANVKGTQTLLEAAREYGISLFLHVSTDEVYGGESIVPLTESAELNPTNPYSKSKAMGEKLARSYWNKYGFPVIVTRGNNVYGPRQYPEKVIPKFISLLQNGRKCCIHGDGSPSRNFMYVDDAATAYLTVLGKGRPGEIYNIGTDFRTSIKELSETIIRKILPQVPDSEYENYIEFVKDRPHNDKSYLIDSSKLENLGWKPLMQWEKGLDITVRWYAKNFDNWPRAEEALAPFPGMNEQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H3C6</accession>
    <name>A0A9Q1H3C6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Zinc finger MYM-type protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_25264</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H3C6"/>
    <dbReference type="OrthoDB" id="10063284at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025398">
        <property type="entry name" value="DUF4371"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008906">
        <property type="entry name" value="HATC_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45749">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45749:SF21">
        <property type="entry name" value="DUF4371 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05699">
        <property type="entry name" value="Dimer_Tnp_hAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14291">
        <property type="entry name" value="DUF4371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4371" evidence="2">
        <location>
            <begin position="150"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="HAT C-terminal dimerisation" evidence="1">
        <location>
            <begin position="673"/>
            <end position="728"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8031904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="753" mass="86133" checksum="A1D238385B3FF0BB" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H4E0</accession>
    <name>A0A9Q1H4E0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NXPE family member 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_25223</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H4E0"/>
    <dbReference type="OrthoDB" id="5950832at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057106">
        <property type="entry name" value="NXPE4_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16165">
        <property type="entry name" value="NXPE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16165:SF5">
        <property type="entry name" value="NXPE FAMILY MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24536">
        <property type="entry name" value="NXPE4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NXPE C-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF24536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8031876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="157" mass="18394" checksum="C5FD8D8FE2266481" modified="2023-09-13" version="1">MEYRHHGLPIRNNWTWIRDIRYIANELDSLKGGDDVIVTLSLWAHFTATSFSFYEQRLRSIKEALNRLWDRAPGTTVVIKGANTRGHSGLSYGLYASDWFAYHLEHILREVFRNDKRIGYLDVWDMTTADLHPDVIHPPAYFVANVVNRLLTYICKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H503</accession>
    <name>A0A9Q1H503_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_26299</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H503"/>
    <dbReference type="OrthoDB" id="6778856at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1820">
        <property type="entry name" value="L1 transposable element, RRM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37445">
        <property type="entry name" value="PROTEIN CBG24663"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37445:SF3">
        <property type="entry name" value="ZINC FINGER PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="7">
        <location>
            <begin position="35"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="101"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="15"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8032700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="307" mass="34522" checksum="EF2E24987991E1C9" modified="2023-09-13" version="1">MAGIDTRADRLKNRSNSSPQLYSPSSGPANENGESKNCGKCNKMVKDNAKAVCCEYCASWYHTRCEGISDEVYKFLNAGGDQIHWFCKSCNSKALDVMKLVQGLKERNEMLEAKLDEVKGQVEEIALVRGSFAEKIREISREEVHEAREKELRECNVVISNIKETDHGAAGDPDQEPVTDNDTVKQLIHNVLGASDVEVVSTTRVPKVKKQGETHNRKLVVKLGSVNQKHKVLRIAKRLHDNDDWQGVYINPDLTKQERKKDYELRSELKRRRENGETNLILSRGQIVTKTNAGASFLHRGHSPAVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H5Y3</accession>
    <name>A0A9Q1H5Y3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_21322</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H5Y3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008528">
        <property type="term" value="F:G protein-coupled peptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009755">
        <property type="term" value="P:hormone-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24372:SF77">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24372">
        <property type="entry name" value="GLYCOPROTEIN HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8034469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="140" mass="15455" checksum="A45913A0C479B3E1" modified="2023-09-13" version="1">MPPFKSPVSAGIAPRQRLTDNYGICCLFANNDGTNCTTPPSPFNTCGSMFPNHVLRATRWLVCLSAMLANLVVIVSRIKAKIPAMRNMFTTEVNTNQNAFLVSLAFADFFMGVYFFSIGIADAKFGEDYFLSAYGWRNGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H6R1</accession>
    <name>A0A9Q1H6R1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Multiple epidermal growth factor-like domains protein 8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_21346</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H6R1"/>
    <dbReference type="OrthoDB" id="263283at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00055">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056737">
        <property type="entry name" value="Beta-prop_ATRN-MKLN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002049">
        <property type="entry name" value="LE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056863">
        <property type="entry name" value="LMN_ATRN_NET-like_EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016201">
        <property type="entry name" value="PSI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46093">
        <property type="entry name" value="ACYL-COA-BINDING DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46093:SF16">
        <property type="entry name" value="MULTIPLE EGF-LIKE-DOMAINS 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24981">
        <property type="entry name" value="Beta-prop_ATRN-LZTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24973">
        <property type="entry name" value="EGF_LMN_ATRN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00180">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00423">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01248">
        <property type="entry name" value="EGF_LAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="9" id="KW-0424">Laminin EGF-like domain</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1035"/>
            <end position="1057"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="13">
        <location>
            <begin position="582"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1100"/>
            <end position="1126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1211"/>
            <end position="1234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="1107"/>
            <end position="1118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1220"/>
            <end position="1234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ8034485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1234" mass="136241" checksum="AB18BFE1344A941E" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H7R7</accession>
    <name>A0A9Q1H7R7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_19232</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8035521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H7R7"/>
    <dbReference type="OrthoDB" id="691673at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8035521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="150" mass="17162" checksum="6CB01F46DAFB9FD5" modified="2023-09-13" version="1">MECDTVNRNVYHKPYSDSSKRQRIEGEGDISPINGPNFCATSQYSGRSFIPVKSSSNTFHSEDVKAEGHTLYGPLSDDTLLNIRNLEQITFGSGTFHIGNRNGAFVRNSEGYTIMLWKFDESARKLFVCRSILFTDRDEYWAVRSRMELM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H8J5</accession>
    <name>A0A9Q1H8J5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_13848</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8039747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H8J5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8039747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="70" mass="8313" checksum="E34BE9A8C7010277" modified="2023-09-13" version="1">MFQKGRSRGMSVKNGRKVLQWELPMWIRSQAMMIPYEEEVEQSVKMNRENLRKIMVMIFSLQFSGSNRAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H8T5</accession>
    <name>A0A9Q1H8T5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF5641 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_17484</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8036840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H8T5"/>
    <dbReference type="OrthoDB" id="5984724at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040676">
        <property type="entry name" value="DUF5641"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18701">
        <property type="entry name" value="DUF5641"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF5641" evidence="1">
        <location>
            <begin position="33"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8036840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="88" mass="10229" checksum="949C70EF1CBF3F02" modified="2023-09-13" version="1">MVQEVKRGMRHLVNGLMFVEWETVFCQISVGEDRKRGSWKMAEDVKLYPGKDGLVRVIDVRFMDGMITSRPVTKLILLMKKLERSDIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H937</accession>
    <name>A0A9Q1H937_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Snaclec 9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_17937</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H937"/>
    <dbReference type="OrthoDB" id="8935730at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051004">
        <property type="entry name" value="DC-SIGN_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22802:SF463">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22802">
        <property type="entry name" value="C-TYPE LECTIN SUPERFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040209714" evidence="2">
        <location>
            <begin position="27"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="3">
        <location>
            <begin position="38"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8037185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="169" mass="20085" checksum="655150155401D243" modified="2023-09-13" version="1">MILFHLHLSQTFALVLFLIFFIKVEGTELECREPFVYFQKYCYYFSSQQMPFRSAKEFCNSYGFPSHPCHLTSIHSSEENSFVHHRATQLWANPIYWLGATKSSNFGYLSWLDRSWVNFTKFRPFGNEGWCVQQTEQQVWDDTNCAHSNYFVCKKVADIAVICKYFVRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HA27</accession>
    <name>A0A9Q1HA27_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_16023</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HA27"/>
    <dbReference type="OrthoDB" id="10068383at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF11">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8038564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="351" mass="40175" checksum="64C1A3B208C02CBB" modified="2023-09-13" version="1">MPPPTTSAISTSSANQSTAVSINLPSLPTFQLHAGNTAVRWDKYIRRLENMFIALNVVNDEQKRALLLHYAGEVMDVFDTLTITGTDYATAKQKLNEYMWPKRNVKYERFAFRETKQLSDESIDVFQTKLQRLASTCDFTNKDAEIKSQMIRGCTSNKLCRLALREDKPPSDLLKLVRSLEISKLHATACELNLVRVVKQISTNAESTDSIVNQYRDLFHGMGKLKDVQCTLHEDKSISPVTQRHRRIPFHVRKLVKEELQRLQALDIIEPVGHEPTPWVSPIEIVKKQKQDDSIRIRVDMREANKAIQRERHVKPTTDDIISDLTGCSVFAKLDLNSGYHQIELDPKSRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HAP2</accession>
    <name>A0A9Q1HAP2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Histone-lysine N-methyltransferase SMYD3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_16544</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HAP2"/>
    <dbReference type="OrthoDB" id="265717at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050869">
        <property type="entry name" value="H3K4_H4K5_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12197:SF251">
        <property type="entry name" value="EG:BACR7C10.4 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12197">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SMYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01753">
        <property type="entry name" value="zf-MYND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01360">
        <property type="entry name" value="ZF_MYND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50865">
        <property type="entry name" value="ZF_MYND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SET" evidence="5">
        <location>
            <begin position="6"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="MYND-type" evidence="6">
        <location>
            <begin position="51"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8038970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="478" mass="54613" checksum="BAAC8C95FE506CAB" modified="2023-09-13" version="1">MTTYLGDVETYQTPDRGRGVTCTSDTKKGTTLLKEECIACVLCSNVRGSYCDHCLLPGYDQLMRCSGCKFARYCNKKCQKNAWIDHKEECKGLKRVAPNIPTDTVRLLARIMHKNRRTPKPVPSIDDKNFPLTIDQLQGDVEKMVEQRREDMGAIFFVLEKFLGGDLLGYTTADILRLFVVVEINGFHLFDHELKNIGYALYQRASMVNHHCEPNCLVVFEGSVLEVRTLQDLKKGDELLITYIETLATTERRRDMLMEQFRFHCMCRKCEDPYSDQVLRCILCPKCSCNGHLKNVSDGTARCSLCGLERSTTDALWEKFTAAEEKAETLLASANEHRSEEKWRDMLLCAEKCLELPYLHPSHFAMLRARNVAMDAHIELQNWQEALTYGEDNLEAYRHQYQFNHPSVGVQLMRVGKLYNYLGKVSEALQMFKQAMDIIGVTHATEHPLRQALSELLISCQMELRGSIMSSITGSDIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HBI7</accession>
    <name>A0A9Q1HBI7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Extracellular serine/threonine protein kinase FAM20C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_14127</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAM20 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8039960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HBI7"/>
    <dbReference type="OrthoDB" id="8583677at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024869">
        <property type="entry name" value="FAM20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009581">
        <property type="entry name" value="FAM20_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12450">
        <property type="entry name" value="DENTIN MATRIX PROTEIN 4 PROTEIN FAM20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12450:SF22">
        <property type="entry name" value="EXTRACELLULAR SERINE_THREONINE PROTEIN CG31145"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06702">
        <property type="entry name" value="Fam20C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0464">Manganese</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FAM20 C-terminal" evidence="9">
        <location>
            <begin position="227"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="320"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="341"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="341"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR624869-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR624869-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR624869-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF06702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8039960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="437" mass="50168" checksum="6B52EC501B3FEA5D" modified="2023-09-13" version="1">MVVSLNQMIPAPLRMCGFRSFRTKAFAFVSTGIAFCLVTFAFELSEHYRTGYTLKGGNRPVNVPAVRGIEVNNSLVNLVTNVKPWDDNVPLIEEVTSLKRETLPTWHQYSSDFSVMELYHPNSSYLPKLLHDLAVVEVRNISTYNKESQFKFLIEFIDGNKAISKPMRYISVSCGRVWGFLRGKGRYGIFDFRKVPPCIGRVLNFTGDIIQKTNDPDILNTQRTKGGQKCIIGGCRNDFCSEKRLTCAKGEFLEVSVCQKIPESIKMRQVKYPWAPSVHDSWKHTNICKNVTKDPIVRKHDLFLNFMEIAVFDHLILNYDRHHFKTIFVNKTKEIGYALFDNGKGFGNPHKDEISLLAPIKQCCRFRKSLYQKVLTLTNEDSKLSSRMRESLSQDPLDPVLTDAFYPALDRRLAQVLSEMNKCLEKFGESKVFSEKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HCA9</accession>
    <name>A0A9Q1HCA9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_11431</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8044067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HCA9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8044067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="178" mass="18761" checksum="4FEE71DA25F80F81" modified="2023-09-13" version="1">MRTKLCITPGLAVLEDENTVPVFIVLLVIVEFPSALAEGLICRPPAVEPSCNVTRDVVKVLSVDKFASATVNCVAPGELAGGLVAVDKTTLETEECIPLEVVGVLPELLAGGLAGWSDFGCVVGVALRLDLLGLSEEDFFGLSDLALEDLDDLEELWLPRFLLPAVGAGTDSEPKAMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HDS6</accession>
    <name>A0A9Q1HDS6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_12320</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HDS6"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040333339" evidence="1">
        <location>
            <begin position="25"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8041491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="104" mass="12607" checksum="9E0AF818C086EEAA" modified="2023-09-13" version="1">MAIPPKRIRLIWLHLHLQRHGVLSVALMRVLRQRQRRTLFGQYDTLMVELAQECGVDYVKFLRRLRMEPHIFRELLERVTPRIQKDQRFAILRIDFNFCAIPTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HID2</accession>
    <name>A0A9Q1HID2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Nicolin-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_06905</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HID2"/>
    <dbReference type="OrthoDB" id="73161at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040235">
        <property type="entry name" value="Nicolin-1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31239">
        <property type="entry name" value="NICOLIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31239:SF2">
        <property type="entry name" value="NICOLIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8047804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="251" mass="28064" checksum="20DBAB5592301172" modified="2023-09-13" version="1">MEVKKGSQLQHIESTTGKPSELKVVSATKLKGDAHGSVSSKRSSGVQVIDVLPAVVPCTIGSISLKNFYTAFLTIRVKIRNEVEKGGVKKDDGKWKTCLHQYQLMPNPHCETGSQDFFTISHKKMLFTPKDVTAVRFILQQPSPVWNSFKIENISLSSDKSEEDTSNSHVLTWLQEQNEISEETAEPQKESDRTAKEIAEHMQRLWALTETVQLQQQTQSSLGRFDVSTDFNNYKNGVVGLEESHNPSTGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HJ34</accession>
    <name>A0A9Q1HJ34_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Complex I-ESSS</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">NADH-ubiquinone oxidoreductase ESSS subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HOLleu_05533</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Nanhai2018</strain>
            <tissue evidence="18">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Complex I is composed of 45 different subunits. Interacts with BCAP31.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complex I NDUFB11 subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HJ34"/>
    <dbReference type="OrthoDB" id="5917019at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019329">
        <property type="entry name" value="NADH_UbQ_OxRdtase_ESSS_su"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13327:SF0">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 BETA SUBCOMPLEX SUBUNIT 11, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13327">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE ESSS SUBUNIT, MITOCHONDRIAL PRECURSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10183">
        <property type="entry name" value="ESSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="13 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="7 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00031387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00046528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ8046753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="165" mass="19122" checksum="5561D5FB6C5235A4" modified="2023-09-13" version="1">MAAAFRCIRPGIMKISQVVRPSLKNLPASSYSSTSKNVKQSDYVIGKPTEVDPKRVEEIIEEGRRAYDEQNDINSPYYLTWGFDAKDNFVDLWSKRIIFFFGVSVVLVLGPVYYHYLPDPKNRKWSHQEAHRVLKERREAGLPLIDPDFVPRDKIILPDDEEEAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HL05</accession>
    <name>A0A9Q1HL05_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_02667</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HL05"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8049770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="53" mass="6082" checksum="F2BB8396079C1B33" modified="2023-09-13" version="1">MPTQHTVQRTCKFITIKPVTTQEESVSIFFSVEVIRGNGKVLEGRRRAGYDNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HLQ6</accession>
    <name>A0A9Q1HLQ6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Zinc finger MYM-type protein 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_03067</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HLQ6"/>
    <dbReference type="OrthoDB" id="6501119at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025398">
        <property type="entry name" value="DUF4371"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46880:SF5">
        <property type="entry name" value="DUF4371 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46880">
        <property type="entry name" value="RAS-ASSOCIATING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14291">
        <property type="entry name" value="DUF4371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4371" evidence="1">
        <location>
            <begin position="43"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8050038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="262" mass="29874" checksum="C2D9A0FCB1BCB9EC" modified="2023-09-13" version="1">MTKVFACLYFLCKQRIAHTTNYEPLLDFIGFLGDKLKEKVFVGRNAHYTSRKSIQKYLKCLSDVLEKDILEELKSSQQFSLMFDETTDCSVREQMVLRARYIHSVNIENIEVRYLKIIDCLTDRHTLDAETISSIVVKYIEDHDLSYKHLKGIGTDGAAVMTGRSNGAVKRIRQKQLSAQNDEEFKCEAVGCHCAAHKLNLASQQAGDHISYILRQLYDFSITVLLGQLAFLQCKNYLDVQKGKSLNHHLHGGSVKAMQSFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0Y8L0</accession>
    <name>A0A9Q0Y8L0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_44795</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010001245">
        <property type="protein sequence ID" value="KAJ8017647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0Y8L0"/>
    <dbReference type="OrthoDB" id="1933708at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon gypsy-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF13">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCAN box" evidence="3">
        <location>
            <begin position="246"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase zinc-binding" evidence="4">
        <location>
            <begin position="798"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="340"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="605"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="704"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="78"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="340"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="355"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="379"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="608"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="628"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="648"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="673"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="720"/>
            <end position="744"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF17921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8017647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="865" mass="98633" checksum="802C90647B5E5B0B" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YAF7</accession>
    <name>A0A9Q0YAF7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_43698</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000419">
        <property type="protein sequence ID" value="KAJ8018355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YAF7"/>
    <dbReference type="OrthoDB" id="6627680at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8018355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="89" mass="9800" checksum="727ED578FDBCDEA7" modified="2023-09-13" version="1">MEGVSYILSDKLNQDPIEEFFGKQRAAGGDRDHPNSRTIWSCLCQEHHSRSSAWACSRKRTKQRVGECAPCVLAVWLCGPPLGVVSSPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YAX1</accession>
    <name>A0A9Q0YAX1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_44168</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000683">
        <property type="protein sequence ID" value="KAJ8018054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YAX1"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8018054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="87" mass="10084" checksum="BCACF1DC45F87A2B" modified="2023-09-13" version="1">MRKLSPPCTYTNRLTNMAACYSLVCKGLDLLKWLGIDKLCEKLVIFSKECKLRSNSSNRMEDCDIVLPFILIFCLKCCRFIVKLGKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YB60</accession>
    <name>A0A9Q0YB60_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_43809</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000471">
        <property type="protein sequence ID" value="KAJ8018281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YB60"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8018281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="58" mass="6868" checksum="E0E0E62A1E087A70" modified="2023-09-13" version="1">MNLWPTSCPCVKLTLMPRVLANIRIMMLYSFVLYLLSALNKMILMSELVNHWMLIRHC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YCR0</accession>
    <name>A0A9Q0YCR0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_42665</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000111">
        <property type="protein sequence ID" value="KAJ8019005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YCR0"/>
    <dbReference type="OrthoDB" id="2801544at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031297">
        <property type="term" value="P:replication fork processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010003">
        <property type="entry name" value="HARP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45766">
        <property type="entry name" value="DNA ANNEALING HELICASE AND ENDONUCLEASE ZRANB3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45766:SF6">
        <property type="entry name" value="SWI_SNF-RELATED MATRIX-ASSOCIATED ACTIN-DEPENDENT REGULATOR OF CHROMATIN SUBFAMILY A-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07443">
        <property type="entry name" value="HARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51467">
        <property type="entry name" value="HARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HARP" evidence="5">
        <location>
            <begin position="217"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="97"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="151"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="102"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="151"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8019005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="345" mass="37917" checksum="94286335DDE347E8" modified="2023-09-13" version="1">MASLSSEQRQRMEQNRLRALQLRAQKNCKQFMPTNSTNAAASKTFENFAKGASSGVQQSELRKNINKPISEVDHSSIAGQLKVSSNISKVSSAILSVSSASGPTNPSASHDSNTRIITSTPGTSFAPANPSHAVSFSSSKPVSQFYKKTLSTGSSSEVNSKNENLKGNPQFQKYNPSSDRPTYQHGGTVLGSRNISATSSNVHFHKQASNSISSKQSSSLMFGHKNSLCASCHLISKDRFMVEMPFQQNVVEIFQKMKSKSYDASSRKWTFGVLEYTDLIKQLRSLRPPVDVKPLPPPIVKLFCTSSKPFPVQEANLISVDQELRDNLLPFQRDGVKYGNFPIFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YFK8</accession>
    <name>A0A9Q0YFK8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Interferon-induced very large GTPase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_40301</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class dynamin-like GTPase superfamily. Very large inducible GTPase (VLIG) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1597724at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030383">
        <property type="entry name" value="G_VLIG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058641">
        <property type="entry name" value="GVIN1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057365">
        <property type="entry name" value="URGCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052986">
        <property type="entry name" value="VLIG_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14819:SF25">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_52, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14819">
        <property type="entry name" value="GTP-BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25496">
        <property type="entry name" value="URGCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25974">
        <property type="entry name" value="URGCP_9th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25683">
        <property type="entry name" value="URGCP_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51717">
        <property type="entry name" value="G_VLIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VLIG-type G" evidence="3">
        <location>
            <begin position="1011"/>
            <end position="1252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="325"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="329"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="346"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8020651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1983" mass="227284" checksum="952C91F5B542813D" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YGY1</accession>
    <name>A0A9Q0YGY1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transcriptional coactivator p15 (PC4) C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_39162</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transcriptional coactivator PC4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YGY1"/>
    <dbReference type="OrthoDB" id="2505440at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060261">
        <property type="term" value="P:positive regulation of transcription initiation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.31.10">
        <property type="entry name" value="Transcriptional Coactivator Pc4, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003173">
        <property type="entry name" value="PC4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009044">
        <property type="entry name" value="ssDNA-bd_transcriptional_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045125">
        <property type="entry name" value="Sub1/Tcp4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13215">
        <property type="entry name" value="RNA POLYMERASE II TRANSCRIPTIONAL COACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02229">
        <property type="entry name" value="PC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54447">
        <property type="entry name" value="ssDNA-binding transcriptional regulator domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcriptional coactivator p15 (PC4) C-terminal" evidence="8">
        <location>
            <begin position="48"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8021849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="144" mass="16296" checksum="12A18DC050F9584C" modified="2023-09-13" version="1">MAGLNAENTSNVSVKQVNSYPSTTQENQGHNRTNFYADLDKSEQVKVDIGQDRFVVVSSFKGKTYIHVRQYYFKKAKCFQGIVFTPKQSSTLVEMIPVVQEAIGKTETGVTEEKYQETLSKRRKTNLKLNFCNKDEAGCSSFFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YGZ2</accession>
    <name>A0A9Q0YGZ2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Long-chain-fatty-acid--CoA ligase</fullName>
            <ecNumber evidence="13">6.2.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HOLleu_37173</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Nanhai2018</strain>
            <tissue evidence="16">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoAs for both synthesis of cellular lipids, and degradation via beta-oxidation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 13">
            <text>(5Z,8Z,11Z,14Z)-eicosatetraenoate + ATP + CoA = (5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19713"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32395"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57368"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.15"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10 13">
            <dbReference type="Rhea" id="RHEA:19714"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 13">
            <text>(E)-hexadec-2-enoate + ATP + CoA = (2E)-hexadecenoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:36139"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:61526"/>
            <dbReference type="ChEBI" id="CHEBI:72745"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11 13">
            <dbReference type="Rhea" id="RHEA:36140"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 13">
            <text>12-hydroxy-(5Z,8Z,10E,14Z)-eicosatetraenoate + ATP + CoA = 12-hydroxy-(5Z,8Z,10E,14Z)-eicosatetraenoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:52112"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:90718"/>
            <dbReference type="ChEBI" id="CHEBI:136408"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8 13">
            <dbReference type="Rhea" id="RHEA:52113"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 13">
            <text>15-hydroxy-(5Z,8Z,11Z,13E)-eicosatetraenoate + ATP + CoA = 15-hydroxy-(5Z,8Z,11Z,13E)-eicosatetraenoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:52116"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:78832"/>
            <dbReference type="ChEBI" id="CHEBI:136409"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9 13">
            <dbReference type="Rhea" id="RHEA:52117"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 13">
            <text>5-hydroxy-(6E,8Z,11Z,14Z)-eicosatetraenoate + ATP + CoA = 5-hydroxy-(6E,8Z,11Z,14Z)-eicosatetraenoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:52108"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:65341"/>
            <dbReference type="ChEBI" id="CHEBI:136407"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6 13">
            <dbReference type="Rhea" id="RHEA:52109"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a long-chain fatty acid + ATP + CoA = a long-chain fatty acyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15421"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57560"/>
            <dbReference type="ChEBI" id="CHEBI:83139"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:15422"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>hexadecanoate + ATP + CoA = hexadecanoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:30751"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12 13">
            <dbReference type="Rhea" id="RHEA:30752"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 13">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.3" evidence="13"/>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YGZ2"/>
    <dbReference type="OrthoDB" id="1700726at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047676">
        <property type="term" value="F:arachidonate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05927">
        <property type="entry name" value="LC-FACS_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045311">
        <property type="entry name" value="LC-FACS_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272:SF107">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 13" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2 13 16" id="KW-0436">Ligase</keyword>
    <keyword evidence="13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="15">
        <location>
            <begin position="95"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="675"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="675"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="713"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="731"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="749"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="770"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="798"/>
            <end position="808"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU369030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ8022315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="821" mass="91003" checksum="3ABCE166A38B7F3A" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YHB3</accession>
    <name>A0A9Q0YHB3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">NLR family CARD domain-containing protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_43162</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000220">
        <property type="protein sequence ID" value="KAJ8018706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YHB3"/>
    <dbReference type="OrthoDB" id="120976at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312">
        <property type="entry name" value="NACHT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312:SF2">
        <property type="entry name" value="NUCLEOTIDE-BINDING OLIGOMERIZATION DOMAIN-CONTAINING PROTEIN 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="483"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="domain" description="Immunoglobulin" evidence="3">
        <location>
            <begin position="26"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="Immunoglobulin" evidence="3">
        <location>
            <begin position="243"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="951"/>
            <end position="974"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8018706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1128" mass="129332" checksum="E2E09D80F48968FC" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YHP4</accession>
    <name>A0A9Q0YHP4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Pancreatic lipase-related protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_39979</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YHP4"/>
    <dbReference type="OrthoDB" id="6431596at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016298">
        <property type="term" value="F:lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013818">
        <property type="entry name" value="Lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000734">
        <property type="entry name" value="TAG_lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610">
        <property type="entry name" value="LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610:SF173">
        <property type="entry name" value="LIPASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00151">
        <property type="entry name" value="Lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Lipase" evidence="5">
        <location>
            <begin position="10"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8020397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="139" mass="15209" checksum="0B6490F42E950C02" modified="2023-09-13" version="1">MGETILYEGNYNVIRVIWDLRPGTDHYAKMSSNARIVGAEIALLIDQLMELAPDGTVAKDAGSYHLIGRGIGAHVAGYAGERHNPKLGRISGLDPAGPYFEDTDLIVRLDHTDAEFVDVIHTDTDPLRRLGKILRTKGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YJ51</accession>
    <name>A0A9Q0YJ51_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Solute carrier family 35 member G1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_35965</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YJ51"/>
    <dbReference type="OrthoDB" id="10553360at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22911">
        <property type="entry name" value="ACYL-MALONYL CONDENSING ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22911:SF137">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER G2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="80"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="110"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="137"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="166"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="200"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="230"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="261"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="287"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="3">
        <location>
            <begin position="19"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="313"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="313"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8023503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="333" mass="36683" checksum="3BC6CE0CCF94FA03" modified="2023-09-13" version="1">MESDRLNCLSQVTLGESLPGILFTLLSALSLSTEDLLLYVVIEDFHPFFIFVFNSNISFILCFLLVLTLRPRRPTTIKQFLLVILHGSLNCVNQISIAASIFAIGPGSAIALFFTVPIFTTVYSALFLRTRLQVKDVFFAICSTLGVALLTRYLTLIENQALNETFSVGFGVARAIAAAMAYAGALIVGRKLAHFETHSLVNVLSYSLQYSIVSIALCSAFNAWEAPKTLSSLAFLLGIGFPTFGGMLFAYLAVCYEKPTIVSIILTSEVIMTFIGQFAMFNFPFHWSVPVGSILIVFSCIGILLFENRKEDMEDGRSKEDENSPIVEKLPSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YJM9</accession>
    <name>A0A9Q0YJM9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Beta,beta-carotene 9',10'-oxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_38722</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="5">Binds 1 Fe(2+) ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the carotenoid oxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YJM9"/>
    <dbReference type="OrthoDB" id="1069523at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003834">
        <property type="term" value="F:beta-carotene 15,15'-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010436">
        <property type="term" value="F:carotenoid dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016121">
        <property type="term" value="P:carotene catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042574">
        <property type="term" value="P:retinal metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004294">
        <property type="entry name" value="Carotenoid_Oase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10543">
        <property type="entry name" value="BETA-CAROTENE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10543:SF24">
        <property type="entry name" value="CAROTENOID ISOMEROOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03055">
        <property type="entry name" value="RPE65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0408">Iron</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="5">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="323"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="525"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR604294-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8021492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="531" mass="59363" checksum="78646D5A9B5E250C" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YKF6</accession>
    <name>A0A9Q0YKF6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Fibrinogen C domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_36712</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8024084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YKF6"/>
    <dbReference type="OrthoDB" id="7871457at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050373">
        <property type="entry name" value="Fibrinogen_C-term_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025615">
        <property type="entry name" value="TILa_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040941">
        <property type="entry name" value="GGGWT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143:SF394">
        <property type="entry name" value="ANGIOPOIETIN-RELATED PROTEIN 3-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143">
        <property type="entry name" value="FIBRINOGEN/TENASCIN/ANGIOPOEITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12714">
        <property type="entry name" value="TILa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00186">
        <property type="entry name" value="FBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="42"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="3">
        <location>
            <begin position="76"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8024084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="162" mass="18483" checksum="924C4F4C63226768" modified="2023-09-13" version="1">MKGDDCVSEMDCGCFVQDEGVIPEGELYVTTNCSNKCECRANELVCTTLNCSEHSTCDVREGARYCYCKKGYHGDGERCVNFIDCLDLFNANFTENGVFMIKPLDWPGAPIKVYCNMTDGGGWTVFQRRLDHSINFYRNWTCYKEGFGSLDHEHWLGNEIIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YKW0</accession>
    <name>A0A9Q0YKW0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Trace amine-associated receptor 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_40323</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YKW0"/>
    <dbReference type="OrthoDB" id="6102451at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00637">
        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050569">
        <property type="entry name" value="TAAR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24249:SF424">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24249">
        <property type="entry name" value="HISTAMINE RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040407752" evidence="11">
        <location>
            <begin position="19"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="65"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="107"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="149"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="204"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="245"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="5"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8020671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="290" mass="33285" checksum="6C8789C5B455D748" modified="2023-09-13" version="1">MILLGNLLIIVLLKSFTCWEDNTCLLLQNLAMTDFGVGATLTLFAIYPSAMSLSQWPYGLFMCKLSSFLGEVCCSASIITLALVSIERYIFIIRPLKYHKYITRRRLIFAIILSWSLPIVLHIATFSNMLGTIYNVNSFLCSSNFPEHLGYTITILAIVFLPANTTIIYTYSCIVSASYRHSRQIHTQQAPPNTNRFSVINHKAICTFLMITGMFNLAWLPYSVLALLRGLLPSTYDFPHWVEFLLPWLAMSNSFVNVFIYLVFNPPIRKHVKKIFNFKMCFNKVFHENL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YLQ5</accession>
    <name>A0A9Q0YLQ5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_34779</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YLQ5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8024773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="81" mass="9190" checksum="09753961FF4236AD" modified="2023-09-13" version="1">MGVRHGFRACKRREVRSQERMQGFMKEGPCTECSEPGSDDGEGIKSLFGHPDFWKIPHETVGFGDSSNVNHRLCPIQKQNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YM07</accession>
    <name>A0A9Q0YM07_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Histamine H3 receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_34976</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YM07"/>
    <dbReference type="OrthoDB" id="5955147at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24228">
        <property type="entry name" value="B2 BRADYKININ RECEPTOR/ANGIOTENSIN II RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24228:SF72">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8024922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="108" mass="12557" checksum="74887F778E56211A" modified="2023-09-13" version="1">MRRLKKQIEISKNLFYVIVIFIIFVAPHLIVEIVNAKPEVINQTKILVVFNSAINPILYGLKHPHFRQVFKCILCRRWKEVPQPSLILKRTGCATAANNTDSHDMQYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YN63</accession>
    <name>A0A9Q0YN63_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">TM2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_33166</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TM2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YN63"/>
    <dbReference type="OrthoDB" id="408511at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007829">
        <property type="entry name" value="TM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050932">
        <property type="entry name" value="TM2D1-3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21016">
        <property type="entry name" value="BETA-AMYLOID BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21016:SF4">
        <property type="entry name" value="TM2 DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05154">
        <property type="entry name" value="TM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="59"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="89"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="TM2" evidence="9">
        <location>
            <begin position="60"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF05154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8025573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="124" mass="13614" checksum="E233C45A081B6D2B" modified="2023-09-13" version="1">MMMMNISSEDGTYGCQFGQDKPNKSEIDPVLVNCTALPGIECHGDRVFQKRVPCVKYDGHHFVTILLFSVFLGFIGLDRFCLGHIPTAVGKLVTIGGLGVWWMIDVVLLVTGQLQPKDGGSWCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YNB6</accession>
    <name>A0A9Q0YNB6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Nuclear pore complex protein Nup50</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_36271</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YNB6"/>
    <dbReference type="OrthoDB" id="10062131at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13170">
        <property type="entry name" value="RanBD_NUP50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000156">
        <property type="entry name" value="Ran_bind_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045255">
        <property type="entry name" value="RanBP1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23138:SF141">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23138">
        <property type="entry name" value="RAN BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00638">
        <property type="entry name" value="Ran_BP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00160">
        <property type="entry name" value="RanBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RanBD1" evidence="2">
        <location>
            <begin position="276"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="165"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="255"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="28"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="97"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="165"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="262"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8023744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="420" mass="44702" checksum="0D0086C57B837E91" modified="2023-09-13" version="1">MAAKRRAGSQLTQDNWQDEEEEDEVVENKPSPFGGFKGFSGTSSSSTNKPAFGSGFQVSSSQPSTLSMTLGVGSKPADSSSIFSLGNQATKPQASVNTALNGGTTSATTNSSSEYCAKLKSLNLAVSSWIQQHVSKTPVCDLTPIFNDYSKHLAEIEEKYGSKSSASASSSVPFGSSDVKSLSTGEADQKTESKPAPSQFTGFSFGSGSSAVKIGEGDDKKESSIFKTSTEGSKLTFGTSSTAFGSSTGQGFTFPGVSVASTEKKEEKKEDTNGEDEPPKVEFKQVEEKDAIFSIRSKLYYKKDGQYKEKGVGMLHLKQTDGVGQILLRADTSLGNIILNIGIPKALPVQRQGKNSVILMAPMNPPVDTACPKCNKKYENPQSSNACENNCTPEICALLLRVKTGDDADKLLEEITKLKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YNJ5</accession>
    <name>A0A9Q0YNJ5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_34638</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YNJ5"/>
    <dbReference type="OrthoDB" id="445826at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8024671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="115" mass="13472" checksum="E28C30A1A67AB38A" modified="2023-09-13" version="1">MFLDLSKAFDTLRSNISFSKLEHNGVRGNVSNWFPSSGFYRQQFSAFSNVNLIIMISVVLILKSVFYFYYFSLLMLMTYTGSQTKCMFLVILMPRIVFIRMLTPVCCLMLFLWNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YNU7</accession>
    <name>A0A9Q0YNU7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_34987</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="KAJ8024926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome 18"/>
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    </dbReference>
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        <property type="entry name" value="SI:CH211-67E16.11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
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        <location>
            <begin position="27"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8024926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="523" mass="60207" checksum="15CED39797317E0D" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
            <fullName evidence="3">Bindin</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="ORF">HOLleu_34542</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
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            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="10072653at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007342">
        <property type="term" value="P:fusion of sperm to egg plasma membrane involved in single fertilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00761">
        <property type="entry name" value="BINDIN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040215762" evidence="2">
        <location>
            <begin position="23"/>
            <end position="1006"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="174"/>
            <end position="212"/>
        </location>
    </feature>
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            <end position="427"/>
        </location>
    </feature>
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        <location>
            <begin position="658"/>
            <end position="853"/>
        </location>
    </feature>
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        <location>
            <begin position="232"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="251"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
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            <end position="379"/>
        </location>
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        </location>
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        </location>
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        </location>
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            <end position="850"/>
        </location>
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        <source>
            <dbReference type="EMBL" id="KAJ8024597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1006" mass="116165" checksum="55CF1538C38FC82B" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Holothuria leucospilota</name>
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        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YR32"/>
    <dbReference type="OrthoDB" id="21416at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="2"/>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="65"/>
            <end position="97"/>
        </location>
    </feature>
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        </location>
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        </location>
    </feature>
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    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YSC1</accession>
    <name>A0A9Q0YSC1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_31977</name>
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    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
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            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
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    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
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    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8026981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="347" mass="39460" checksum="5BF65C5E004DCFED" modified="2023-09-13" version="1">MEPGSYHSSLNDSITKIYRKANPQDVSNLNQEAKRIAKSLNLDNRIDQLAEHEAFISLKDHKENFANKPVCRQISPTKSDIVQISKHILDKVIKTIRTKTKVNLWKSTRDILQWYNSITDKPNHSFVVFEIVEFYPSITENLLLKALHYADQLHRITPEEINIILHARRTNVFSSNAQWRKKNNDSLFDIAMGSLDGAECCELVVCSLLSLLKPKYGNSLGLYTDDGLGAFNCPLRELENIKKDICKTFRENDLKITAEANKKNVNYLDVTLDLPTSSYRPVADPGGSPASGPPSNFPMCPFSKNMFFFHTSKKKKKKNETVSQVMTVLSNSEEILVIPIQYRSIHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1B8G0</accession>
    <name>A0A9Q1B8G0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_45153</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010001968">
        <property type="protein sequence ID" value="KAJ8017436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1B8G0"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8017436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="52" mass="5924" checksum="E0F820D34BB87E2A" modified="2023-09-13" version="1">MPHIERKNPIVFGRGQRSPGVTGGQTLKTLLTQYLEVGRLDEFHTLFVDALW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BA60</accession>
    <name>A0A9Q1BA60_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_44486</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000926">
        <property type="protein sequence ID" value="KAJ8017853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BA60"/>
    <dbReference type="OrthoDB" id="5982640at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8017853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="235" mass="27273" checksum="4AD5B2BE5C2B6AB0" modified="2023-09-13" version="1">MNEKNFIDWFFTGDNSKNLIAALNNVSFKRYKLEGPFVRLEKSPAHIYGVVINEDGQHEHPFWKELYRLGLKLVKIGFTQQETIKGTGNRMEIIQKQIKSELGFESSPIFCLMIDFTTTASFTEVEEDIRSKVGIKLDKEFAKWLKLPIPTEWVLTSQEMISSLTNRIEEKKKQCSSNKTIYTTKDMEPCDLQSIEKLVNEISLSYVGLKDNKIVMRTEEEVLVENFKKALKQFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BAB6</accession>
    <name>A0A9Q1BAB6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_44201</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000705">
        <property type="protein sequence ID" value="KAJ8018033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BAB6"/>
    <dbReference type="OrthoDB" id="8963689at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="78"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8018033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="184" mass="21877" checksum="29D3264406E87F4F" modified="2023-09-13" version="1">MPFNLDKFVASPSVEELDSLKKSEIVKVAKHYGVEFQPLMRKDEIKRYVLEYLVDESILPITVLETAITVPTDNTFELKRLEIEMNKEIRLKRNEEREMQMQMQKEKEEREMQMQMQREKEAREHEFRLKQLELGVIKGSDPKIGLDTGGLFDVSKHVKFVPKFQEDNVEKFFNHFEKLGEQLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BB75</accession>
    <name>A0A9Q1BB75_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_43230</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000241">
        <property type="protein sequence ID" value="KAJ8018664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BB75"/>
    <dbReference type="OrthoDB" id="6244150at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8018664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="263" mass="29904" checksum="D2C44BAF62C9048A" modified="2023-09-13" version="1">MSDAGGPASEPPPKEPPSGEGWFDLCEGVETESKLKRSASARDFNCVPTHSSTECYTHIFELLVVGIAELDMFTKNHDMVDVWRVQHPGLAVFTWHRPDGTDASRLDRVYSPPALSPSVCEVVACPISDHDAVTVSFQPPESWPAGRGLWKMNTDILTEAEYCKQFKARYEGWRTLRPAFSTDLEWWDDVKSRIKHFTVEYCVARARRRREEFLSLCSRERNGDPSALHAIQQYLDEKLHGAHHQVLPGRHKVCHRSPHKKSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BBS7</accession>
    <name>A0A9Q1BBS7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Acyl-coenzyme A oxidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">HOLleu_40576</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">Nanhai2018</strain>
            <tissue evidence="20">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism; peroxisomal fatty acid beta-oxidation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the acyl-CoA oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BBS7"/>
    <dbReference type="OrthoDB" id="538336at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003997">
        <property type="term" value="F:acyl-CoA oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005504">
        <property type="term" value="F:fatty acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033540">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA oxidase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.540.10:FF:000006">
        <property type="entry name" value="Acyl-coenzyme A oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000005">
        <property type="entry name" value="Acyl-coenzyme A oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000013">
        <property type="entry name" value="Acyl-coenzyme A oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.110.10:FF:000003">
        <property type="entry name" value="Acyl-coenzyme A oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055060">
        <property type="entry name" value="ACOX_C_alpha1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029320">
        <property type="entry name" value="Acyl-CoA_ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012258">
        <property type="entry name" value="Acyl-CoA_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002655">
        <property type="entry name" value="Acyl-CoA_oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10909">
        <property type="entry name" value="ELECTRON TRANSPORT OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10909:SF250">
        <property type="entry name" value="PEROXISOMAL ACYL-COENZYME A OXIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01756">
        <property type="entry name" value="ACOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22924">
        <property type="entry name" value="ACOX_C_alpha1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14749">
        <property type="entry name" value="Acyl-CoA_ox_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000168">
        <property type="entry name" value="Acyl-CoA_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 13 15" id="KW-0274">FAD</keyword>
    <keyword evidence="8" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5 13" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-coenzyme A oxidase N-terminal" evidence="18">
        <location>
            <begin position="32"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase C-alpha1" evidence="19">
        <location>
            <begin position="292"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase C-terminal" evidence="17">
        <location>
            <begin position="491"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="14">
        <location>
            <position position="438"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR000168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000168-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000168-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF01756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF14749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF22924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAJ8020870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="681" mass="76950" checksum="43CD03BEEFEB8393" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BDD5</accession>
    <name>A0A9Q1BDD5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Carcinine transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_38442</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BDD5"/>
    <dbReference type="OrthoDB" id="2544694at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="19"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8021288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="202" mass="23090" checksum="F01FD86C90420BF0" modified="2023-09-13" version="1">MDFNYALNSVGKFGKYQKILTFLLCLPILIECMHFYVQVFAAGESDHWCQSWKDENFKDLNLSDIQCKNPKKETSVPVKVTNGDEIVFEQCEKYNASGTDLETAMLAEKNNYTTPEKIPCDEGWIHNKSTFPSTIIIDLAITIPYVPVLVLEFLFVLESPLWFIAKENIEEADKVLDKVAIRNGKKCTDSILKSFESNTEVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BFM7</accession>
    <name>A0A9Q1BFM7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Alpha-2A adrenergic receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_33722</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8026001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BFM7"/>
    <dbReference type="OrthoDB" id="10010417at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14967">
        <property type="entry name" value="7tmA_amine_R-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000929">
        <property type="entry name" value="Dopamine_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248">
        <property type="entry name" value="ADRENERGIC RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248:SF125">
        <property type="entry name" value="DOPAMINE D2-LIKE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00242">
        <property type="entry name" value="DOPAMINER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 10" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 10 14" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="33"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="71"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="112"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="154"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="195"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="320"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="359"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="53"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="273"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="273"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="290"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="300"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ8026001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="403" mass="45269" checksum="349B369BB465819B" modified="2023-09-13" version="1">METEPPYLITTDDDVTLLTTQETLQEDHQEISVLFLIFKIVILGSLIVCGVVGNTLVVISVFKFRTLRIVANYFIVSLAAADLMVSFLVMPMALHQEITQGHWKLGAGICDLWVAVDVLTCTSSIWNLCSISVDRFLAINHPIQYAIRRTPTMAAIVIISMWLFCFMISIPALVAVGGFDESKGQGECALNVSPIFQISSSMLSFYFPCFILLFVYYKIYRSVQALSKRRPGEKVPDKKRVLRENASKVETLTTKVDTMASHVDQMDTLKVHTQPNGSGSSCMSELPHTEATSQQPTKSISKRTGHQTTKRISVAKERRATSVLAIVVTVFICCWLPFFITNVLIGIGCNITFQTFNAVTWLGWANSAANPLIYTIFNREFRHAFRKILTCRINQHSPYNKRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BHH7</accession>
    <name>A0A9Q1BHH7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">adenylate cyclase</fullName>
            <ecNumber evidence="4">4.6.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HOLleu_35011</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Nanhai2018</strain>
            <tissue evidence="18">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP = 3',5'-cyclic AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15389"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <dbReference type="EC" id="4.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.1" evidence="4"/>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BHH7"/>
    <dbReference type="OrthoDB" id="10006362at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
        <property type="term" value="F:adenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006171">
        <property type="term" value="P:cAMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07556">
        <property type="entry name" value="Nucleotidyl_cyc_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1230:FF:000014">
        <property type="entry name" value="adenylate cyclase type 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018297">
        <property type="entry name" value="A/G_cyclase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032628">
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        <property type="entry name" value="ADENYLATE CYCLASE"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR45627">
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
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    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="GUANYLATE_CYCLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0115">cAMP biosynthesis</keyword>
    <keyword evidence="13 14" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="180"/>
            <end position="198"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="210"/>
            <end position="229"/>
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    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="235"/>
            <end position="257"/>
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    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="264"/>
            <end position="282"/>
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    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="288"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="318"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="17">
        <location>
            <begin position="408"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
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        <location>
            <begin position="11"/>
            <end position="21"/>
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    </feature>
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            <begin position="26"/>
            <end position="54"/>
        </location>
    </feature>
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            <begin position="83"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00012201"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ8024947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="541" mass="61206" checksum="CD2448FE33D16B99" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BHZ8</accession>
    <name>A0A9Q1BHZ8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">E3 ubiquitin-protein ligase RNF38</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_33288</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
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    <dbReference type="GO" id="GO:0061630">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="RING finger protein 44 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Znf_RING"/>
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        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
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        <property type="entry name" value="GH10160P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46171:SF3">
        <property type="entry name" value="GH10160P"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="608"/>
            <end position="649"/>
        </location>
    </feature>
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        <location>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="412"/>
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        </location>
    </feature>
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        <location>
            <begin position="56"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="121"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="149"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
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    </feature>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="276"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8025667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="659" mass="73284" checksum="65538D4F251F3857" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BID9</accession>
    <name>A0A9Q1BID9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Sushi, von Willebrand factor type A, EGF and pentraxin domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_31458</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Virion</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="5804959at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
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        <property type="entry name" value="CCP"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.960">
        <property type="entry name" value="SEA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051503">
        <property type="entry name" value="ComplSys_Reg/VirEntry_Med"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000082">
        <property type="entry name" value="SEA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036364">
        <property type="entry name" value="SEA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45785">
        <property type="entry name" value="COMPLEMENT FACTOR H-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45785:SF2">
        <property type="entry name" value="COMPLEMENT FACTOR H-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01390">
        <property type="entry name" value="SEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82671">
        <property type="entry name" value="SEA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="19"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 5" id="KW-0768">Sushi</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1562"/>
            <end position="1588"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="8">
        <location>
            <begin position="126"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="8">
        <location>
            <begin position="246"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="8">
        <location>
            <begin position="307"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="8">
        <location>
            <begin position="368"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="8">
        <location>
            <begin position="429"/>
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        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="8">
        <location>
            <begin position="490"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="8">
        <location>
            <begin position="551"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="8">
        <location>
            <begin position="612"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="8">
        <location>
            <begin position="673"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="8">
        <location>
            <begin position="736"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="8">
        <location>
            <begin position="797"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="8">
        <location>
            <begin position="858"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="8">
        <location>
            <begin position="919"/>
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        </location>
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    <sequence length="1690" mass="185129" checksum="CAE8A1261757D742" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BNF9</accession>
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        <recommendedName>
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        </recommendedName>
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                <person name="Chen T."/>
            </authorList>
        </citation>
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            <strain evidence="4">Nanhai2018</strain>
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        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
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                <person name="Chen T."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain evidence="10">Nanhai2018</strain>
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        </source>
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        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="component" value="Chromosome 13"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="8" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
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    <keyword evidence="1" id="KW-0596">Phosphopantetheine</keyword>
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        <location>
            <begin position="48"/>
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        </location>
    </feature>
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    </evidence>
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    </evidence>
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    </evidence>
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        </source>
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        </source>
    </evidence>
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        </source>
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        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAJ8030138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="271" mass="29846" checksum="1D18B08116CD0CBC" modified="2023-09-13" version="1">MNIFKNGCWGSYRHQFLNEAHNFIFIPPFPEELFTTPKNAYVDVLTPGDFSSLQWIQLPGEPKSGEKYTVHYSSLNFRDIMLASGKMPRGSLPGKIMISSVENLAHKECLIGIEFAGHNSEGKRVMGIVGSQGLACHIFAEPCFLFDVPDSWTLEDAATVPLAYCSVFYALVVRGQVKAGESILIHSGSGGVGQAAIAVALSYGCKIYTTVGGYIVTFNVLSTTEKTEITYPSTTTPHQAYFNIFYLLEFHFLHRNAGETKLSHAEISNVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BNR3</accession>
    <name>A0A9Q1BNR3_HOLLE</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_29637</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8030062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BNR3"/>
    <dbReference type="OrthoDB" id="5835829at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8030062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="122" mass="13694" checksum="8A9B0433BA21050E" modified="2023-09-13" version="1">MTFNFLLDNVDSSNVLFFNGMGEGSHFAAAAAIGEELASRGHQVTFLVSNEFKHLAENGKFLSNFEFIIIDSEVLMTPYYAQITQAQFEGLDLYQNKTFTDEVDEARAQECSFVFSSNLLKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BQG4</accession>
    <name>A0A9Q1BQG4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_27364</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BQG4"/>
    <dbReference type="OrthoDB" id="10564941at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="138"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8030833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="191" mass="20724" checksum="C14897942E7F518E" modified="2023-09-13" version="1">MAHPRELSKTTFIVCMGGIALWVLILPATHVYGLPYGPHPPETESGFSFKNFKDFVLAGITPETHTHDPAEIYMHKGQEPAVGEVGVATPAEGQKLLVENELGVNNEGGADSPLSNEGQPAKIVLVAGEDGDAIQEKQHKQEEEAVETLDVPHEHPKMEFSDEIGEKNLVAGEQVMKELPDAKVMAAREEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BRR2</accession>
    <name>A0A9Q1BRR2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_24837</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BRR2"/>
    <dbReference type="OrthoDB" id="10618297at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="266"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="331"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="334"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8031597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="491" mass="54574" checksum="86045DFF49FCC77E" modified="2023-09-13" version="1">MDLVSRIVFTSFLVTGYSSFGTPLDVSFELVDSDINEIIFVNETDIVLQCCIAGEANNYSLSIEHENRDDPVLLSRNDNCLKYVIKKVTMKDGGEYTCNVSFICEDGSTQTTAKTLYLNIQDGIRPQCFRNGTAGQPYNDGDLLLMTCYCRFIDRSCSWISSVPGTRRASPVTPYETKNRFDKMILRLLVKVGSSNSTISTTMYHCSNGPAFTQRCEIGPQMASSTDVIINSYTEDTFTDTLDCVLPSTTTLTSQEEGLDATNHEIIVVGASVFIGIVLVVIIILIIVFTLLKSRRKVHSDKEVRDPDTNLVYANCSFTTDNIDQIDLNKDRSSTVNDNSQRHVSQSDCMKRGAGSADFGDLYAKIDKSMKKSGNNIPEMETASANHISSAYKPDSATLYAKIDKDRRKHGDEMLSMEPTSEHHNASEGVEDFACLYAKVDKDRRNFGGEADEGRCQKNSDDSDMYSEVNKKLGLTPISFMTRQYTLKADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BRZ2</accession>
    <name>A0A9Q1BRZ2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Alcohol dehydrogenase [NADP(+)] A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_24939</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BRZ2"/>
    <dbReference type="OrthoDB" id="416253at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19071">
        <property type="entry name" value="AKR_AKR1-5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43827">
        <property type="entry name" value="2,5-DIKETO-D-GLUCONIC ACID REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43827:SF14">
        <property type="entry name" value="NADP-DEPENDENT OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00063">
        <property type="entry name" value="ALDOKETO_REDUCTASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8031675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="179" mass="20053" checksum="61875952BADB8887" modified="2023-09-13" version="1">MEALVERGLTKSVGVSNFNVTQLSDVLKATTVPCVVIQIENHPFLDQKELIDLCKSKNVTVTSYSPLGSPDRPCVHILLFAQFRASDKDPSLLTHPVIKEMSETKGCTPGQLLIAYHLEQGLVCIPKSVTPSRIQENFGALDVKLTDEDIKKLDDTCCNFRACTWYMWKGHIHYPFEDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BSA6</accession>
    <name>A0A9Q1BSA6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Receptor-type tyrosine-protein phosphatase beta</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_25329</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BSA6"/>
    <dbReference type="OrthoDB" id="10253954at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050991">
        <property type="entry name" value="ECM_Regulatory_Proteins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46708:SF2">
        <property type="entry name" value="FIBRONECTIN TYPE-III DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46708">
        <property type="entry name" value="TENASCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0675">Receptor</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="2">
        <location>
            <begin position="85"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="2">
        <location>
            <begin position="178"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="2">
        <location>
            <begin position="440"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="2">
        <location>
            <begin position="623"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="2">
        <location>
            <begin position="751"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="2">
        <location>
            <begin position="853"/>
            <end position="960"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8031952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="983" mass="106355" checksum="1BEEEF94688111D3" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BTA7</accession>
    <name>A0A9Q1BTA7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Cubilin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_25736</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BTA7"/>
    <dbReference type="OrthoDB" id="9971251at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000005">
        <property type="entry name" value="Procollagen C-endopeptidase enhancer 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052129">
        <property type="entry name" value="Spermadhesin-Link_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46908:SF8">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46908">
        <property type="entry name" value="CUBILIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="159"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="4">
        <location>
            <begin position="10"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="4">
        <location>
            <begin position="244"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8032259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="364" mass="40483" checksum="8021BDBDC32D757F" modified="2023-09-13" version="1">MVYLRGFFTCIVQHTEGSDTFTSAQYPDTGTVDDSWEITVDQKHQILLQFLDFSLNETTSCTADVLELYNGDRFDNVLIDRFCWDDATVLTSTAYVSTGHQLYMRLITAEGYSRKGFRAHYTSVDLQHTNSSGKITSPGYPNTGHPLDEAWRIKVNEGFVVGILAVVVFTVFVIAIIVWKFKIKKAKSSPPTGQAIQSQMAGNPGTYEDVNLQKNAINRASLETSHEKKSVMNLSTTAYVEPCCIVPHTDKSGTITSSQYPGTGAVNDAWEITVDQGHQILLQFLDFSLNETTSCTVDVLELYNGGESDNVLIDRFCWKDATVLASTAYVSTGNQLYVRLKTADGYSRKGFRAQYTSGKCHRKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BTS6</accession>
    <name>A0A9Q1BTS6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transcription intermediary factor 1-beta</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_26207</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BTS6"/>
    <dbReference type="OrthoDB" id="10113904at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047153">
        <property type="entry name" value="TRIM45/56/19-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25462">
        <property type="entry name" value="BONUS, ISOFORM C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25462:SF296">
        <property type="entry name" value="MEIOTIC P26, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101898">
        <property type="entry name" value="NHL repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="B box-type" evidence="3">
        <location>
            <begin position="17"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="181"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8032635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="649" mass="73943" checksum="96945FE5FBA842D4" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BTX4</accession>
    <name>A0A9Q1BTX4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Laminin subunit alpha</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_23247</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
            <location evidence="1">Basement membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BTX4"/>
    <dbReference type="OrthoDB" id="18487at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005604">
        <property type="term" value="C:basement membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005201">
        <property type="term" value="F:extracellular matrix structural constituent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009887">
        <property type="term" value="P:animal organ morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009888">
        <property type="term" value="P:tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00055">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000011">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000090">
        <property type="entry name" value="laminin subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000388">
        <property type="entry name" value="Laminin subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000034">
        <property type="entry name" value="Laminin subunit alpha 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050440">
        <property type="entry name" value="Laminin/Netrin_ECM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002049">
        <property type="entry name" value="LE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10574:SF406">
        <property type="entry name" value="LAMININ SUBUNIT ALPHA 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10574">
        <property type="entry name" value="NETRIN/LAMININ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00053">
        <property type="entry name" value="EGF_laminin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00011">
        <property type="entry name" value="EGFLAMININ"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00180">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01248">
        <property type="entry name" value="EGF_LAM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50027">
        <property type="entry name" value="EGF_LAM_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0084">Basement membrane</keyword>
    <keyword evidence="7 10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 10" id="KW-0424">Laminin EGF-like domain</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Laminin EGF-like" evidence="12">
        <location>
            <begin position="123"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="12">
        <location>
            <begin position="170"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="12">
        <location>
            <begin position="215"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="12">
        <location>
            <begin position="261"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="12">
        <location>
            <begin position="318"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="123"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="125"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="144"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="170"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="190"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="215"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="217"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="236"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="288"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="341"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8033108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="581" mass="63234" checksum="D2B5DC328290F5C0" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BV10</accession>
    <name>A0A9Q1BV10_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">Armadillo repeat-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_23556</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">In association with mitochondrial contact site and cristae organizing system (MICOS) complex components and mitochondrial outer membrane sorting assembly machinery (SAM) complex components may regulate mitochondrial dynamics playing a role in determining mitochondrial length, distribution and motility.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Interacts with mitochondrial contact site and cristae organizing system (MICOS) complex components IMMT/MIC60 and MICOS10/MIC10. Interacts with mitochondrial outer membrane sorting assembly machinery (SAM) complex components SAMM50 and MTX1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BV10"/>
    <dbReference type="OrthoDB" id="17335at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016617">
        <property type="entry name" value="ARMC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46840">
        <property type="entry name" value="ARMADILLO REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46840:SF2">
        <property type="entry name" value="ARMADILLO REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF013899">
        <property type="entry name" value="UCP013899"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ARM" evidence="7">
        <location>
            <begin position="28"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="8">
        <location>
            <begin position="148"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00046478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR013899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8033347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="286" mass="31772" checksum="0C851A44DB3A7D2A" modified="2023-09-13" version="1">MAAQGVVQQLRDLAADPQNRRKIVQDQGCLPGLVLLLDHNEDQVVVTALEALRFLAECSSNRPIMKAELGMMFSLEAIMHNTTSSNRAKMLAKQVYQKLTARCVTPTPLQESRNTAINQQQQMKKQPTVKPSKGFFLGTNKNNKVVILQIKGLKDQVCRKACEEELLKTKGVISFTFDLLKGRCTLRVKKEMRTETLVAAISRTKIMSADQVLKNEQGEEIILSFGSKPALANKENAALPDYLPEEDSPIRGLDKAVTKPGMVGDEDKQASWLSTAASFISSSFYW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BX06</accession>
    <name>A0A9Q1BX06_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_20924</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BX06"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8034190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="87" mass="9826" checksum="DD8AED031883F2F9" modified="2023-09-13" version="1">MDTLRKGHKVLERTILYYCTWGIGNHLVSTFRRFEPATENSGTKRSLMAEGVIKLFSLYTKRCKNPPGYFCNASKTAPAISPSLNLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BXS4</accession>
    <name>A0A9Q1BXS4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_21801</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BXS4"/>
    <dbReference type="OrthoDB" id="6343797at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00024">
        <property type="entry name" value="CD_CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46585:SF1">
        <property type="entry name" value="CHROMO DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46585">
        <property type="entry name" value="INTEGRASE CORE DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="2">
        <location>
            <begin position="1"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="Chromo" evidence="1">
        <location>
            <begin position="240"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8034802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="446" mass="51878" checksum="AA1E0E998D58E047" modified="2023-09-13" version="1">MDLADLSSLSRYNKGYKFLLTCIDVLSKFAWVIPLKNKSGRTLLQAVKKILSSGRKPQQIHTDRGTEFVNKLLQKYLKDKGIHFFTTNNETKASVVERFNRTLKSKMWKYFTHRNTLKYIDILPNLVKGYNHSYHRSIKLRPVEVTKDNEDRVWRTLYPGINIKASFKFNVGDRVRISKSKLKFEKGYLPNWSEEIFIIVKRKGKPVPVYKLTDWGGEPIEGNFYESELQKVNLAQGALFRVEKVLKKRKRNGRVEYLVKWKGYPTKFNSWVTDNFYVTLPSNSSFQYFPENTLASFKTQLAEPIVLNGSWEVSLCEIQYPFNWHNVTEHNCRVQLNDREICIPPGYYDTIQDVIDAFHDLLLEEERRHVKLFQHKHNLRIAVRLGPGFTLAFQGMSHMFGFLSANKVCTEGFTVSDISSDVHSGVTSFFVYIESLQLSDVSLDHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BYL6</accession>
    <name>A0A9Q1BYL6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">52 kDa repressor of the inhibitor of the protein kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_22455</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BYL6"/>
    <dbReference type="OrthoDB" id="10051013at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052958">
        <property type="entry name" value="IFN-induced_PKR_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46289">
        <property type="entry name" value="52 KDA REPRESSOR OF THE INHIBITOR OF THE PROTEIN KINASE-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46289:SF14">
        <property type="entry name" value="DUF4371 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="578"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="598"/>
            <end position="610"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8035282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="630" mass="70236" checksum="5CBE4F2954DE4090" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BZC6</accession>
    <name>A0A9Q1BZC6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Ankyrin repeat and MYND domain-containing protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_19439</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8035687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BZC6"/>
    <dbReference type="OrthoDB" id="10257049at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000182">
        <property type="entry name" value="Ankyrin repeat and MYND domain containing 2a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052452">
        <property type="entry name" value="Ankyrin-MYND_dom_contain_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24150">
        <property type="entry name" value="ANKYRIN REPEAT AND MYND DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24150:SF8">
        <property type="entry name" value="ANKYRIN REPEAT AND MYND DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01753">
        <property type="entry name" value="zf-MYND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144232">
        <property type="entry name" value="HIT/MYND zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01360">
        <property type="entry name" value="ZF_MYND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50865">
        <property type="entry name" value="ZF_MYND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0969">Cilium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="repeat" description="ANK" evidence="9">
        <location>
            <begin position="43"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="9">
        <location>
            <begin position="77"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="MYND-type" evidence="12">
        <location>
            <begin position="317"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="365"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="365"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8035687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="431" mass="48791" checksum="877D7CC94F9406C0" modified="2023-09-13" version="1">MKKKGELSDSERALFENVQNGNTLVVKELLSKDDVDVNCLDDYGMTPLQNTAFKANVEMCQILLSHGADVNKNEHENGYTTLMFAALSGNTEVTKMMLDAGAKTSPVNSVGRNACQMAAFVGQHECVTVIKNFFPSDELNYYTVPHGFEKEPKLRTDLAPTLHKFILCNNVSPVKLALFLKENMELVNETPKITKVLEHICEKQMKASETNDVLALKMHYLVQVLEACRKWDVEKTDVGIDGFIKVLLKERESDGVPIGMEKFIRDAIREFPYHDSELFVQVVRNLADTQPGREPSALVMLTQSINGTRSIEIEDLCSACNEPKAEKKCSACKYVRYCNQSCQKLHWFTHKKRCKELGEKLKKAEAEKIRREEEERKRKQKEAEEAARQKEEESKGEDSKEKEGEGAITSESQETDKTTPPVEDDVQKPES</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C0E8</accession>
    <name>A0A9Q1C0E8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_19823</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8035978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C0E8"/>
    <dbReference type="OrthoDB" id="6122973at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028027">
        <property type="entry name" value="SPMAP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34221:SF1">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34221">
        <property type="entry name" value="HYPOTHETICAL PROTEIN LOC691189"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8035978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="220" mass="25883" checksum="869B50B6C5B1A769" modified="2023-09-13" version="1">MSHPHVIYGSPLVHVDRINHVVRPNPTKEQLKRLEDEFILDCIITEKLRYKWSPDIIPKYDARFDHHAAAYFKSPTQIQLRHHASSPIKPFPFRRPQLDKLTLSGRRRLPPEKTAKELEEKRVNFVNNHVFLTNQVTKDKPLIDPYNAIFDNHSAKYFQKADVKKLLSYTMPSGIHPKVRLRWLDARVHVRTEENGLQHAVMVAKDLRTIIPLKLPYRKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C0X5</accession>
    <name>A0A9Q1C0X5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_20549</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C0X5"/>
    <dbReference type="OrthoDB" id="419189at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8036542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="144" mass="15845" checksum="4AF3DFAAD6D60364" modified="2023-09-13" version="1">MIRNTASPGPKGRSWNHLGTVCTHPVGKIIRSNNIGYLINADDTQMYVTFDPKIPGACNDALCKLQSCIKQIKDWMVVNRLQLNQSKTGFFISGSSRSISRLNTVILDLGDVQITPATSLRNLGLMFDTCLPMTDPMSSIVKLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C1T9</accession>
    <name>A0A9Q1C1T9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">4-aminobutyrate aminotransferase, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_17808</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>4-aminobutanoate + 2-oxoglutarate = succinate semialdehyde + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:23352"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57706"/>
            <dbReference type="ChEBI" id="CHEBI:59888"/>
            <dbReference type="EC" id="2.6.1.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C1T9"/>
    <dbReference type="OrthoDB" id="5419315at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034386">
        <property type="term" value="F:4-aminobutyrate:2-oxoglutarate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009450">
        <property type="term" value="P:gamma-aminobutyric acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000073">
        <property type="entry name" value="Probable 4-aminobutyrate aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43206:SF1">
        <property type="entry name" value="4-AMINOBUTYRATE AMINOTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43206">
        <property type="entry name" value="AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000521">
        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="5 7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8037076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="366" mass="40742" checksum="DA63FD12A1C372CF" modified="2023-09-13" version="1">MYPNMEYPDKLEKLVKTIAPKGLKHMQTLMCGSCSNDTAIKHAFIAYRHRERGGNPTEEDLFSAMRNQAPGTPNYTVLSFESGFHGGTFACLSLSHTVQVYKVDVPAADFPCAPFPRFKYPLKEFKTENRAEEDRCLVRVREIIDERKQAGGPVAAVIVEPIQAEGGDFHATPYFFRNLQQICKETGAALIVDEVQTCCGGTGHFWAHEAWDLPEAPDFVTFSKKLISGGYFFKEEFDPKFGERIFNTWMGDPSKVLMMEAAVDVIKRDGLLELIRESGACLQAGLEDIQNKYPQHLHSARGSATFCAINTTDSALQPTLAASLRNKGVLVGTCSTNTLRFRPALIFQPHHAEIILDTLESAVKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C540</accession>
    <name>A0A9Q1C540_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Balbiani ring protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_15707</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C540"/>
    <dbReference type="OrthoDB" id="9981160at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004153">
        <property type="entry name" value="CXCXC_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03128">
        <property type="entry name" value="CXCXC"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040437992" evidence="4">
        <location>
            <begin position="18"/>
            <end position="836"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8038319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="836" mass="95409" checksum="EF0BC08A56812F63" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C7G0</accession>
    <name>A0A9Q1C7G0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transmembrane protein 56</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_15092</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C7G0"/>
    <dbReference type="OrthoDB" id="10266980at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006634">
        <property type="entry name" value="TLC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050846">
        <property type="entry name" value="TLCD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13439">
        <property type="entry name" value="CT120 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13439:SF0">
        <property type="entry name" value="TOPOISOMERASE I DAMAGE AFFECTED PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03798">
        <property type="entry name" value="TRAM_LAG1_CLN8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00724">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50922">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="97"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="177"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="222"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="TLC" evidence="7">
        <location>
            <begin position="50"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8040713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="265" mass="30866" checksum="0D666EB4E64BBAA1" modified="2023-09-13" version="1">MESYDDLFQINYMYSLVAASSFTFFTVMFYIVSPWFSKKISSQYETLDLYVQHDWNSRVIAVIHASVVTPLAIYVVLIDEEAKRDPVWGNSLVARTAMALTEGYMISDFIIVLRYFPLKDAVIYSFHHIATLYPYTYNVLYGPMPYFGCFKLATEGSTPFVNIRWFLSTLKRSDSDLYYYNGIIMTGLFFMLRVVPIIPFWVAIYGTFTTHPMQGITWEMRGSLVIGSLILDLLNLYWFRKMLHGAKKVMNRRSLVKEEMKNKGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C8Q7</accession>
    <name>A0A9Q1C8Q7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Histamine N-methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_14467</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C8Q7"/>
    <dbReference type="OrthoDB" id="5984880at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8040235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="293" mass="33469" checksum="FA0319ADD729B4C2" modified="2023-09-13" version="1">MSLRYLQNFPSHYWKSYRSMRDSFYECPLEYDIGDEAFQKMVVDVLPKQESLKALGIGSGQGKLDCIHMGRMLTRVSRIDNTVVEPSLEAVEVYKSLLSGDLSDRVTTSWYQETFQQYQKRRESSTGDTEKFHFISVVHSLYYTGNDGSSIGYLIDLLEENGVLFIAIQNDESGFIKFFRKAVESYGPRYNNTTRGYTTSQVVKLLNADGRVNYNVVKNRHKIDVTACFEADSSKGSLVLDFLTHVVDFTSTFSKNVLQSLLDYLKSPDCSEILSDGRIILHLKWDAIIITKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C9E4</accession>
    <name>A0A9Q1C9E4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_14754</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C9E4"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8040459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="211" mass="24079" checksum="CAD96552099FB352" modified="2023-09-13" version="1">MTLLSRDHFQSEVDSQYECHLSEPDEDSTVSSLRTSHTRACRNDFRDGCDPILLDELNPEPPFPHRVPGNRDTDPLSYFVVDMINDDKNNAFGVFACRATNVEKVETTISTTRMRSDAIIIPADDLFTKTVNINDTNVTISVRYIGSDDQPALRWRNNTQFRNDESVYFNTDTFILDEPAQLYHKGIYESFIAGNRNETRHALIILIVRGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C9K5</accession>
    <name>A0A9Q1C9K5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="1">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_14974</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4 10">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="1"/>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C9K5"/>
    <dbReference type="OrthoDB" id="6144703at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00047">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000102">
        <property type="entry name" value="Receptor-type tyrosine-protein phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050348">
        <property type="entry name" value="Protein-Tyr_Phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134">
        <property type="entry name" value="RECEPTOR-TYPE TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134:SF449">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040239463" description="protein-tyrosine-phosphatase" evidence="5">
        <location>
            <begin position="23"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="6">
        <location>
            <begin position="225"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="7">
        <location>
            <begin position="400"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="6">
        <location>
            <begin position="520"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="7">
        <location>
            <begin position="701"/>
            <end position="777"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8040629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="808" mass="93191" checksum="EFBBE59214B8D37A" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C9R1</accession>
    <name>A0A9Q1C9R1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_15419</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C9R1"/>
    <dbReference type="OrthoDB" id="10020599at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8040957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="175" mass="19517" checksum="DEC28A510D50E2E8" modified="2023-09-13" version="1">MKAEPALPERDSNLKLARFAFRYFFSTEVSNIRLGFHTQTDQEIVKYEESGKLISSLKEVKPTCVDEVLAAISGCSNSTCDLNPILSSLLKKCDVQLAPVFTEKVNSSFATSIFPSSLKTAIVKPLLEEASLDKEIMDNCLPVSYLPFLSKVIEKIATSRINGHLMENNLHEKMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CAL0</accession>
    <name>A0A9Q1CAL0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Nudix hydrolase 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_12729</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Nudix hydrolase family. PCD1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CAL0"/>
    <dbReference type="OrthoDB" id="206213at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010945">
        <property type="term" value="F:coenzyme A diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008893">
        <property type="term" value="F:guanosine-3',5'-bis(diphosphate) 3'-diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015938">
        <property type="term" value="P:coenzyme A catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034654">
        <property type="term" value="P:nucleobase-containing compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009132">
        <property type="term" value="P:nucleoside diphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090407">
        <property type="term" value="P:organophosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03426">
        <property type="entry name" value="NUDIX_CoAse_Nudt7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.79.10:FF:000036">
        <property type="entry name" value="Nudix hydrolase 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045121">
        <property type="entry name" value="CoAse"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000059">
        <property type="entry name" value="NUDIX_hydrolase_NudL_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12992">
        <property type="entry name" value="NUDIX HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12992:SF24">
        <property type="entry name" value="PEROXISOMAL COENZYME A DIPHOSPHATASE NUDT7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01293">
        <property type="entry name" value="NUDIX_COA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0464">Manganese</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="187"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="224"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="Nudix hydrolase" evidence="9">
        <location>
            <begin position="38"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8041816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="259" mass="29021" checksum="2A3B36530EE8A2A3" modified="2023-09-13" version="1">MYSSSFDAAIPKDEVRRNLQKQDSGDFLSLNPQLDWKGTRAAVLVPLFYVQDQLHVLLTRRADGLRTAPGEVAFPGGKRDKSDKDDIKTALREAEEEIGLPQNEVEVLSRLPPLFSVNSVSATPVVAFVPNDCLSRIAPNPSEVQDVFSVPLAMFLSEKQHRSFATNWPGRFEFIHFFQESKKQSSPVIFGLTAYICICVASIVHNRDPDFQMEITFNRKDMEIGYLVAFSVLSIATHMNVAEKMRKKLSGVTAQNSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CBJ6</accession>
    <name>A0A9Q1CBJ6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_09478</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8042663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CBJ6"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8042663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="149" mass="17031" checksum="133BD96D7EFB1CA2" modified="2023-09-13" version="1">MESLIPELPSSPVYKDDSDLESSPKARQESHKGEHLKNDTRIRLEDDSYKRVKYHLAPLTKIQSTVIGLTNEIKKLESDLAEGRIPTHLRVSRSLPRLPGYLSCSGELRDSWSSLLQRAGRKLGRLRARSCLDRQSGNEIDLRRRNDPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CDX9</accession>
    <name>A0A9Q1CDX9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">LIM/homeobox protein Lhx1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HOLleu_09601</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Nanhai2018</strain>
            <tissue evidence="15">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8042754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CDX9"/>
    <dbReference type="OrthoDB" id="10068367at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09367">
        <property type="entry name" value="LIM1_Lhx1_Lhx5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09375">
        <property type="entry name" value="LIM2_Lhx1_Lhx5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000120">
        <property type="entry name" value="Insulin gene enhancer protein ISL-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000075">
        <property type="entry name" value="LIM/homeobox protein Lhx1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000046">
        <property type="entry name" value="LIM/homeobox protein Lhx1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049618">
        <property type="entry name" value="Lhx1/5_LIM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049619">
        <property type="entry name" value="Lhx1/5_LIM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050453">
        <property type="entry name" value="LIM_Homeobox_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24208:SF105">
        <property type="entry name" value="DLIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24208">
        <property type="entry name" value="LIM/HOMEOBOX PROTEIN LHX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 9 11" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5 10" id="KW-0440">LIM domain</keyword>
    <keyword evidence="2 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="13">
        <location>
            <begin position="3"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="13">
        <location>
            <begin position="63"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="14">
        <location>
            <begin position="239"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="9">
        <location>
            <begin position="241"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="141"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="360"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="465"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="157"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="205"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="226"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="364"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="390"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="476"/>
            <end position="485"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ8042754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="496" mass="54880" checksum="9D8A49E0A43ECB7F" modified="2023-09-13" version="1">MVQFCAGCEGPIMDRFLLNVLDRPWHIKCVQCCECKAKLTEKCFSREGKLFCKTDFFRRYGTKCAGCSQGILPSDLVRRARGKVFHLNCFTCMVCRKKLSTGEELYIVDENQFICKDDYNGPCDPTGATLPMSHPSNYDNRPYHINYPLSDPGIPSPENSSPENMIESDSNDGSPSSTPVPSQGLGQGGNSTRSSTPLADNGTDDVEKTKEIKREDSETPGKDSENGSTPPGSTPVSGAKRRGPRTTIKAKQLETLKAAFAATPKPTRHIREQLAQETGLNMRVIQVWFQNRRSKERRLKQLSALGARRNHFFRHQRRMRPLRGPFDLDGNPDMMSPPFNYHEPDQSHPDFFGPQGYFDIFPPGHHHPHHPHAPHHNGQNPDPGSDMGPYMPPHPQIPPGTPMTSVEQPLPPHSMHGPMEGPQFLHPTKEMNGTNTDSMQPMSTGANGDLLTIGDRYTSLPPRSVSNPVSMQNGGPPNGVIGGNGYRVPEVADGAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CE69</accession>
    <name>A0A9Q1CE69_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Adhesion G protein-coupled receptor L3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_09953</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CE69"/>
    <dbReference type="OrthoDB" id="671595at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19941">
        <property type="entry name" value="TIL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.220.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053066">
        <property type="entry name" value="ADGR_G7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057244">
        <property type="entry name" value="GAIN_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046338">
        <property type="entry name" value="GAIN_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000203">
        <property type="entry name" value="GPS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47767">
        <property type="entry name" value="ADHESION G PROTEIN-COUPLED RECEPTOR G7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47767:SF1">
        <property type="entry name" value="ADHESION G PROTEIN-COUPLED RECEPTOR G7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01825">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00303">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50221">
        <property type="entry name" value="GAIN_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="486"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="525"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="558"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="domain" description="GAIN-B" evidence="7">
        <location>
            <begin position="292"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="8">
        <location>
            <begin position="488"/>
            <end position="581"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8043029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="581" mass="64768" checksum="F911B8DCFB92525E" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CEN2</accession>
    <name>A0A9Q1CEN2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_10092</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CEN2"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00637">
        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24246:SF27">
        <property type="entry name" value="ADENOSINE RECEPTOR, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24246">
        <property type="entry name" value="OLFACTORY RECEPTOR AND ADENOSINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="50"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="85"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="118"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="161"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="196"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="242"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="66"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8043129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="341" mass="39118" checksum="BCA7A96AD765D8D0" modified="2023-09-13" version="1">MEPTTEDALPFKFETTQEDLRFINQTEKPQTFHPPALPPGWTNEQFHKLLILYEIRMVLCGIGLLANSLILLLQLKLKTFKKPQLIFAFTLTATDGIVCPAVFMQTYYYRNNVLSVPLLYHVITFATTIMSLLLITAVACDRYLALCAIPLKYKLWVNSRRYTFVIIVMCALSTGYGWLFGWFFTFENDFSTGLRTFIVSIILIGTTTIIYIFVYFSVSRSLKKLSLPTAIKNARIRQTRRLMFAFALILGTNVICSMPQPLFGLYIAFQPIGQGYLLFKNNVISNLLYNVQCLNFTLNPVIYWWQVLLRDLSLPCRRNNTRLKANSSSPIQEVATVTSQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CF25</accession>
    <name>A0A9Q1CF25_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP-dependent DNA helicase</fullName>
            <ecNumber evidence="1">5.6.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_10557</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.3" evidence="1"/>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CF25"/>
    <dbReference type="OrthoDB" id="10071389at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010285">
        <property type="entry name" value="DNA_helicase_pif1-like_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051055">
        <property type="entry name" value="PIF1_helicase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47642">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47642:SF5">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05970">
        <property type="entry name" value="PIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0227">DNA damage</keyword>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="1" id="KW-0234">DNA repair</keyword>
    <keyword evidence="1 3" id="KW-0347">Helicase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA helicase Pif1-like DEAD-box helicase" evidence="2">
        <location>
            <begin position="12"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8043464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="190" mass="21480" checksum="26098109AB534D57" modified="2023-09-13" version="1">MQSISCFPDQQIHAFLTGGAGVGKSVLIDTLFQALHIFLGGLAGENPDDQRILLCAPTGKAAYNISGVTIHSAFKINPNQGFNFKKISSDQLNTLRVRYRHLSVVIIDEISMVGNKQFLFMHLRLQEIKTSPKPFGGIHMIAVGDFFQLKPFKDQCIFMNLKDGYGPLTTNLWKEYFHMHELQHHKILQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CF30</accession>
    <name>A0A9Q1CF30_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Fucosyltransferase</fullName>
            <ecNumber evidence="11">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_11320</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Endomembrane system</location>
            <topology evidence="10">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Golgi apparatus</location>
            <location evidence="11">Golgi stack membrane</location>
            <topology evidence="11">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the glycosyltransferase 10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="11"/>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CF30"/>
    <dbReference type="OrthoDB" id="427096at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046920">
        <property type="term" value="F:alpha-(1-&gt;3)-fucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11660:FF:000002">
        <property type="entry name" value="Alpha-(1,3)-fucosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11660">
        <property type="entry name" value="Glycosyl transferase family 10, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055270">
        <property type="entry name" value="Glyco_tran_10_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031481">
        <property type="entry name" value="Glyco_tran_10_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001503">
        <property type="entry name" value="Glyco_trans_10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038577">
        <property type="entry name" value="GT10-like_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11929">
        <property type="entry name" value="ALPHA- 1,3 -FUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11929:SF145">
        <property type="entry name" value="ALPHA-(1,3)-FUCOSYLTRANSFERASE FUT-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17039">
        <property type="entry name" value="Glyco_tran_10_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00852">
        <property type="entry name" value="Glyco_transf_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="domain" description="Fucosyltransferase N-terminal" evidence="13">
        <location>
            <begin position="151"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="Fucosyltransferase C-terminal" evidence="12">
        <location>
            <begin position="252"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00060399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF17039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ8043987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="478" mass="56553" checksum="AA8CB958724E0A6A" modified="2023-09-13" version="1">MTSNFRTYLFLFFQVSFTIVLICLYYAYNTEYSSHSFAYPAISSIVPERQFDPDEGISYYEEIIQEHNEFYVDTPEKAVTDINIRPAYRGSDHLCLSRTRSVSCLKRDKTQCLKVSFVGLCGRWSSTLRQFPFEVTCKGTPQRKILVDHVDKPKAIENAQIVIFSFVASYLNMDVWLALRRFRPRGQMWVFQTEESPYYAVGLLPPWNFRNDSYNTSITFHSAADIQSPYGYYEPFRNEESNILENFTEILAAKKNFMIWISSHCETLHWDRTRFVKDLQRSMRVDIFGRCGNDSLCKTRWQDNCVQEVDDFVKSYKFILSLENSCCEDYVTEKLWHSFTVGTVPVVIGPRIENYERLAPPGSFIHVDQFSSVESLRDYLKHLDKNDTAYLEYFKWKKLGKVREHTIKEHYQNHVSDENICTVVDQYFERQNSAEKLKMFDPYGPRWKGLCHQCGRNSWIQNYQFPLDHERKNPWIWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CFK6</accession>
    <name>A0A9Q1CFK6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Baculoviral IAP repeat-containing protein 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_07194</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2196114at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004869">
        <property type="term" value="F:cysteine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032465">
        <property type="term" value="P:regulation of cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00022">
        <property type="entry name" value="BIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23810">
        <property type="entry name" value="UBCc_BIRC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000014">
        <property type="entry name" value="Baculoviral IAP repeat-containing protein 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1170.10">
        <property type="entry name" value="Inhibitor Of Apoptosis Protein (2mihbC-IAP-1), Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BIR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022103">
        <property type="entry name" value="BIRC6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
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        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46116">
        <property type="entry name" value="(E3-INDEPENDENT) E2 UBIQUITIN-CONJUGATING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46116:SF39">
        <property type="entry name" value="BACULOVIRAL IAP REPEAT-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00653">
        <property type="entry name" value="BIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12356">
        <property type="entry name" value="BIRC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00238">
        <property type="entry name" value="BIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57924">
        <property type="entry name" value="Inhibitor of apoptosis (IAP) repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50143">
        <property type="entry name" value="BIR_REPEAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="4">
        <location>
            <begin position="4598"/>
            <end position="4765"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="407"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="831"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1029"/>
            <end position="1050"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1579"/>
            <end position="1610"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1645"/>
            <end position="1665"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="2133"/>
            <end position="2171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="2362"/>
            <end position="2408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="2509"/>
            <end position="2532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="3635"/>
            <end position="3670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="3932"/>
            <end position="3956"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="4015"/>
            <end position="4040"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="4467"/>
            <end position="4496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="4525"/>
            <end position="4544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="4835"/>
            <end position="4884"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="408"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="851"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1591"/>
            <end position="1600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1645"/>
            <end position="1654"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="2136"/>
            <end position="2146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="2149"/>
            <end position="2171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="2370"/>
            <end position="2386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="2387"/>
            <end position="2402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="3654"/>
            <end position="3670"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="3932"/>
            <end position="3942"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="3946"/>
            <end position="3956"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="4027"/>
            <end position="4040"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="4469"/>
            <end position="4483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="4525"/>
            <end position="4534"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8044441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="4942" mass="540222" checksum="562C2D5F73BADF2C" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CFT7</accession>
    <name>A0A9Q1CFT7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Protein dispatched-like 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_11246</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the dispatched family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CFT7"/>
    <dbReference type="OrthoDB" id="193905at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007224">
        <property type="term" value="P:smoothened signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1640.10">
        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052081">
        <property type="entry name" value="Dispatched_Hh_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053958">
        <property type="entry name" value="HMGCR/SNAP/NPC1-like_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000731">
        <property type="entry name" value="SSD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45951:SF3">
        <property type="entry name" value="PROTEIN DISPATCHED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45951">
        <property type="entry name" value="PROTEIN DISPATCHED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12349">
        <property type="entry name" value="Sterol-sensing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82866">
        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50156">
        <property type="entry name" value="SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="33"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="325"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="348"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="428"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="456"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="537"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="794"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="823"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="851"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="887"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="925"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="domain" description="SSD" evidence="8">
        <location>
            <begin position="348"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8043928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="987" mass="111197" checksum="26FC266FC634E148" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CIB7</accession>
    <name>A0A9Q1CIB7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">EH domain-binding protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_08217</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CIB7"/>
    <dbReference type="OrthoDB" id="5972258at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000023">
        <property type="entry name" value="EH domain-binding protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022735">
        <property type="entry name" value="bMERB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050540">
        <property type="entry name" value="F-actin_Monoox_Mical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019448">
        <property type="entry name" value="NT-C2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23167">
        <property type="entry name" value="CALPONIN HOMOLOGY DOMAIN-CONTAINING PROTEIN DDB_G0272472-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23167:SF46">
        <property type="entry name" value="EPS15 HOMOLOGY DOMAIN CONTAINING PROTEIN-BINDING PROTEIN 1, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12130">
        <property type="entry name" value="bMERB_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10358">
        <property type="entry name" value="NT-C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01203">
        <property type="entry name" value="DUF3585"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BMERB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51840">
        <property type="entry name" value="C2_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0967">Endosome</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2 NT-type" evidence="8">
        <location>
            <begin position="8"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="7">
        <location>
            <begin position="411"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="domain" description="BMERB" evidence="9">
        <location>
            <begin position="834"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="257"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="558"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="969"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="257"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="272"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="308"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="349"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="558"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="596"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="641"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="701"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="811"/>
            <end position="837"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8045248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1015" mass="115480" checksum="37811EAD2441B4D8" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CIS7</accession>
    <name>A0A9Q1CIS7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNA-directed DNA polymerase from mobile element jockey</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_08350</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CIS7"/>
    <dbReference type="OrthoDB" id="7480422at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33332">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8045354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="360" mass="41503" checksum="E94FE4BA3C7DD6E3" modified="2023-09-13" version="1">MKNFPDMPESVFPEMPKICIDQKGVENLLLNLNPNKAAGPDAIPAKILRECASSLAPVLTKIFQKSLDSGCLPEAWLDANVTPLYKKGDRCIPADDCILYSKINTTEDSKRLQKDLDSLLSWEKDWQMSFNASKCFTLRVTHKKVPITNNYMMGDTVLEEVKHHPYLGVELSNDLKWSTHISQITTKANKMLGLLKRNIYYCSKSTRSIAYKSLVRPKLEYCSAICDPKHKLDRDKLEEIQNRAARFTVRDYSRDSSITKILADLEWESLHVRRTRSRLITIYKETHGLIPSNIKSFLQSPRSEMHPHTRQTGYLKYNFITTNKDCYRYSLYPRTIPEWNLLDLDTCIAPDVNSFKEKLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CJX3</accession>
    <name>A0A9Q1CJX3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="2">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_04942</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="2"/>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CJX3"/>
    <dbReference type="OrthoDB" id="265776at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06466">
        <property type="entry name" value="p23_CS_SGT1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06463">
        <property type="entry name" value="p23_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02674">
        <property type="entry name" value="Peptidase_C19R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007052">
        <property type="entry name" value="CS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF74">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04969">
        <property type="entry name" value="CS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01753">
        <property type="entry name" value="zf-MYND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144232">
        <property type="entry name" value="HIT/MYND zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51203">
        <property type="entry name" value="CS"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01360">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50865">
        <property type="entry name" value="ZF_MYND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 6" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="7"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="CS" evidence="10">
        <location>
            <begin position="115"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="8">
        <location>
            <begin position="314"/>
            <end position="1050"/>
        </location>
    </feature>
    <feature type="domain" description="MYND-type" evidence="9">
        <location>
            <begin position="606"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="212"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="690"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="751"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="224"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="296"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="692"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="757"/>
            <end position="766"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8046311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1052" mass="119600" checksum="7E8D9230E3FADA22" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CKW8</accession>
    <name>A0A9Q1CKW8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_05296</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CKW8"/>
    <dbReference type="OrthoDB" id="6143625at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8046580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="622" mass="71244" checksum="9779569ED971E06F" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CL78</accession>
    <name>A0A9Q1CL78_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Transmembrane protein 43</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_05791</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus envelope</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TMEM43 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CL78"/>
    <dbReference type="OrthoDB" id="410725at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005637">
        <property type="term" value="C:nuclear inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071763">
        <property type="term" value="P:nuclear membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012430">
        <property type="entry name" value="TMEM43_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13416">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13416:SF2">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07787">
        <property type="entry name" value="TMEM43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="318"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="360"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="383"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="17"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8046945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="414" mass="46619" checksum="BEEFCEA70A129CEF" modified="2023-09-13" version="1">MHRAKQSSDIHLGGMRGDSHPNNKPSEDSISKKNVQFLRGISDTFGGGMAGIGLIFIGVFVLFLNESSIVISSQRLADKLNTIADVPSLYNILQENNNRLVYISGPLKTNHPLTDGQFGIEAHALKLRREVQMFQWVEHRRAVPSGSNEPTDISTVYTKEWRSDLIDSDSFIKPERHQNKKDFPVKSTTFVATHAYVGSYYIKEALKEKIEEFSPLPPIQPKEDARIQMVDGFYYQTGVSLHPDIGDVRARFFYCGASGQEDNVLGPPQELSILAKQVNGQLTRFRKGNDRLDVVHFGKETVRELVIKELNIKTNLGWTLRVAGFVFLLLGFMLANNIMYKIASTVPEVQDFFLVNPLRFHLLASFCLLVVITILCWISLKVVFLLFAFVVVFIPFVMIIKFGSDSSKKASSKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CN50</accession>
    <name>A0A9Q1CN50_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_00115</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8047976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CN50"/>
    <dbReference type="OrthoDB" id="6249720at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8047976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="93" mass="10413" checksum="781E141DAD47AB66" modified="2023-09-13" version="1">MNFAPTVLRVKSPPKELITATEYRIKDLSIGEADLIRCDVLRILKKAKMPKSSISHKEKVALSELKSDDSIIILPADKGRSTVVHCNKQRTVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CPA2</accession>
    <name>A0A9Q1CPA2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_00324</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CPA2"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8048144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="138" mass="15158" checksum="9E8035728E026361" modified="2023-09-13" version="1">MAVTGHKNESSVRSYVNRLSIGQKRKLSESLSGMSAQLATGGATVSNTSTANSLMLTNMANCNAVIQQGVHGETERFTNVSNSSTHVTMSFDEQRQSTSLSSLFHNCNFRAPVNVTITSHYKLLLSYLVKNLVFYLQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CR26</accession>
    <name>A0A9Q1CR26_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_02278</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CR26"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002218">
        <property type="term" value="P:activation of innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042509">
        <property type="entry name" value="ZCCHC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22639">
        <property type="entry name" value="GAG-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22639:SF3">
        <property type="entry name" value="ZINC FINGER CCHC DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="144"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="180"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="197"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="247"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="280"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="248"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8049506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="716" mass="79250" checksum="558F3908EB0BCCA4" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CS19</accession>
    <name>A0A9Q1CS19_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_02739</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CD225/Dispanin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CS19"/>
    <dbReference type="OrthoDB" id="6083617at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051423">
        <property type="entry name" value="CD225/Dispanin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007593">
        <property type="entry name" value="CD225/Dispanin_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14948:SF25">
        <property type="entry name" value="DUF4190 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14948">
        <property type="entry name" value="NG5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04505">
        <property type="entry name" value="CD225"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="16"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8049823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="131" mass="14620" checksum="C1B38C9414527B6B" modified="2023-09-13" version="1">MKINYAFFYFTSTEPPYNRLDEDQGKQELPAQQGYPPQQEYPPQYGYSMVNTAENQQICLTPTSTVVTRVTQVQPDDHLALSIFVLICCCLPVGIVAVIKASEVCMARSHCKNDHGSTTSIVLLSCSKTYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CTJ4</accession>
    <name>A0A9Q1CTJ4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">GTPase IMAP family member 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_04177</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. AIG1/Toc34/Toc159-like paraseptin GTPase family. IAN subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CTJ4"/>
    <dbReference type="OrthoDB" id="2386367at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000840">
        <property type="entry name" value="Immune-associated nucleotide-binding protein 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006703">
        <property type="entry name" value="G_AIG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045058">
        <property type="entry name" value="GIMA/IAN/Toc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903:SF184">
        <property type="entry name" value="GTP-BINDING PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903">
        <property type="entry name" value="GTPASE, IMAP FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04548">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51720">
        <property type="entry name" value="G_AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIG1-type G" evidence="5">
        <location>
            <begin position="31"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="354"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="456"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="354"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="372"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="457"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8050830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="556" mass="65172" checksum="208994A6AEF86603" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CTK5</accession>
    <name>A0A9Q1CTK5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_03388</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CTK5"/>
    <dbReference type="OrthoDB" id="10040707at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8050254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="94" mass="10711" checksum="F9F4079EDF9A7CBD" modified="2023-09-13" version="1">MASSICLQPGTAKTFQGYATDVFVPYVTFQLQQYVDRLDIVWDLYMADSLKANTRSKRGKGVRRRVEPSSAVPENWQKYSPHRLQDGVVLLLGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CTN2</accession>
    <name>A0A9Q1CTN2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">CMP-N-acetylneuraminate-poly-alpha-2, 8-sialyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_03949</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 29 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CTN2"/>
    <dbReference type="OrthoDB" id="10264956at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003828">
        <property type="term" value="F:alpha-N-acetylneuraminate alpha-2,8-sialyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006491">
        <property type="term" value="P:N-glycan processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009311">
        <property type="term" value="P:oligosaccharide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23963">
        <property type="entry name" value="GT29_ST8SIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1480.20">
        <property type="entry name" value="Glycosyl transferase family 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001675">
        <property type="entry name" value="Glyco_trans_29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050943">
        <property type="entry name" value="Glycosyltr_29_Sialyltrsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038578">
        <property type="entry name" value="GT29-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11987">
        <property type="entry name" value="ALPHA-2,8-SIALYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11987:SF52">
        <property type="entry name" value="CMP-N-ACETYLNEURAMINATE-POLY-ALPHA-2, 8-SIALYLTRANSFERASE-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00777">
        <property type="entry name" value="Glyco_transf_29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8050660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="351" mass="41007" checksum="A9230968F8719B7E" modified="2023-09-13" version="1">MSIHSAFCVFPIMRKSAYLVSLALVMFVGLAFRLRGLKIGYSPTFDFSSCDQLCGSSQEDCHDRDMTVVFYQRVHPSKMNFKQMNQYYVRNLTLSDMLRVGGWSSKQKPSIRKCYVTDIDPSLSTFKRHRRCAIVGNSGILLKSRCGNAIDSHDFVIRANLAPTNGYSDDVGHKTDLMLINDETLRHIHSSFSVREWEEHKIPLLHQLRDLKDTILWYPKDATKYNEELRYIGDNFQRQRISSSVGFSLKDIYSDIKWKYNLWRYPSSGLYMLALAESLCDKVSLFGFYPYETDDYGERVLRHYYEPDLKHFTTWAHNFDAEYKMLQSRRDKGLLEMFTSPCVPKDNIEKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H1N9</accession>
    <name>A0A9Q1H1N9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transposase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_26594</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H1N9"/>
    <dbReference type="OrthoDB" id="6348118at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33053">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8030239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="410" mass="46192" checksum="975C471DFD03B5EB" modified="2023-09-13" version="1">MEYYFLGLCNQLEMIFATRNLEDSFIDLILNVDGVPLFKSSGSTLWPVLGAIANISPPHVFPIALTYGHSKPENLDFLSDTISELSDLLENGIHIDDNNVTVTLKGIICDAPARAFIKLSKQYSGYYGCDKCSQKGEWVGRMTYPIAKEFEKRTDGSFRDKANEEHHHGTSPFCDLPIDMVKCFPVDYMHQACLGVMRKLLLIWIRGKGRLLDVRMSAAHVARVSEKLVAMKCSVPSEFQRKPRSLTEIDRWKATELRQCLLYTGKLCFEGILRKDLYDHFMALSVATCILACPSHASKYSEYASELMIYFNEQGRVLYGPEFLVYNVHSMVHLADEAAEFGNLDACSAFAFENYLQRLKRLVRSGKQPLIQIASRLSERCGMQVSNHDAGVVSVSVSKPNNCYILSDTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H1S1</accession>
    <name>A0A9Q1H1S1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">von Willebrand factor A domain-containing protein 3A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_25299</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H1S1"/>
    <dbReference type="OrthoDB" id="6241467at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032770">
        <property type="entry name" value="DUF4537"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14343:SF5">
        <property type="entry name" value="DUF4537 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14343">
        <property type="entry name" value="VWFA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15057">
        <property type="entry name" value="DUF4537"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13768">
        <property type="entry name" value="VWA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VWFA" evidence="2">
        <location>
            <begin position="23"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="571"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="723"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="920"/>
            <end position="1039"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="571"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="738"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="937"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="963"/>
            <end position="995"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1015"/>
            <end position="1024"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1027"/>
            <end position="1039"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8031932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1057" mass="120237" checksum="9867DEF6411CD07C" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H2U3</accession>
    <name>A0A9Q1H2U3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_26900</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H2U3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040224506" description="Ig-like domain-containing protein" evidence="2">
        <location>
            <begin position="21"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="390"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8030465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="612" mass="67697" checksum="9F59E96109BC52B1" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-04-02" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H330</accession>
    <name>A0A9Q1H330_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Endonuclease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_27223</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="775972at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05481">
        <property type="entry name" value="retropepsin_like_LTR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09274">
        <property type="entry name" value="RNase_HI_RT_Ty3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000023">
        <property type="entry name" value="Pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000003">
        <property type="entry name" value="Protein CBG25708"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.370:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 17.6-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000063">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 297-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041577">
        <property type="entry name" value="RT_RNaseH_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF7">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17919">
        <property type="entry name" value="RT_RNaseH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="212"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase" evidence="4">
        <location>
            <begin position="498"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase catalytic" evidence="5">
        <location>
            <begin position="1050"/>
            <end position="1215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="240"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1299"/>
            <end position="1360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="250"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1299"/>
            <end position="1321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8030735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1375" mass="156336" checksum="42B8B4C1EF56EADD" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H3U4</accession>
    <name>A0A9Q1H3U4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_25916</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H3U4"/>
    <dbReference type="OrthoDB" id="10030269at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8032399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="100" mass="10825" checksum="CCB99956ABB341C5" modified="2023-09-13" version="1">MASVLQYMFPPVGNADFGKQTTKVKNSITGSPCLSETSRILLQGASHSENTALLFQCAVSLAMRGITVMYITHQKFTSLPASVEGAPRPDPILMKQILIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H642</accession>
    <name>A0A9Q1H642_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_21532</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H642"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8034619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="107" mass="12266" checksum="27D2259D0050B820" modified="2023-09-13" version="1">MESSREFNFNAAFLELSRRENCLRGLTTLTLLRSYSQPLTLHICDKLPYAVSTLCTEIVIIHHSSPSLVIIRSWNQFLRYSTLQVYYCTIVNVHALPALGPDQTHIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H9V6</accession>
    <name>A0A9Q1H9V6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_18440</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H9V6"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="131"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8037591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="167" mass="19182" checksum="09D89B0EE58EC2B7" modified="2023-09-13" version="1">MQRRSRRCSRTVGIITYHARANTQKFRSSNFNRKAKTVILFPFTSLANATYASVGDIRFLHAYTALALPLCFCPAHHHCIMAGDQPQSINNNLNTDVDRERTGDQCQKKENEERETRQHNRASRQAVYSGKEAKVQPEKMPEDLRSTEKQPCNHPKFPYHPCHSPIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HB03</accession>
    <name>A0A9Q1HB03_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_16238</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HB03"/>
    <dbReference type="OrthoDB" id="10059413at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1820">
        <property type="entry name" value="L1 transposable element, RRM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004244">
        <property type="entry name" value="Transposase_22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11505">
        <property type="entry name" value="L1 TRANSPOSABLE ELEMENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8038731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="139" mass="16282" checksum="B7F1E0C962F3C07E" modified="2023-09-13" version="1">MDTNKLERFSRRNNFRIVGIPVTENENCEESVKEKVFPLFKDAPDLHIERCHRDGRAPPGRSPHILVRCLSYKAKSFIMRYRRSALQGQSFFIVDDLTRLDLAEKKKWSSKVKDLYERGVKLTFSGGKWRDATGKPFEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HCG3</accession>
    <name>A0A9Q1HCG3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA-directed DNA polymerase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_15411</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HCG3"/>
    <dbReference type="OrthoDB" id="5988713at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.960.10">
        <property type="entry name" value="VSR Endonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33206:SF1">
        <property type="entry name" value="DNA-DIRECTED DNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33206">
        <property type="entry name" value="PROTEIN CBG10425"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8040950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1003" mass="117684" checksum="24F40F5EC7533507" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HD17</accession>
    <name>A0A9Q1HD17_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_15241</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HD17"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8040828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="87" mass="9465" checksum="E1F133EA42A72307" modified="2023-09-13" version="1">MGAPAVCERSCGYGCTQQHVLNRTRYSRRELRSRTSFLAHAELETGVAARKFRRCNEKSRAAARACLGKQPALQTRTSAGLVGGQPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HDQ5</accession>
    <name>A0A9Q1HDQ5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_12355</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HDQ5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8041518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="105" mass="11843" checksum="E5C6B3C17DE22E87" modified="2023-09-13" version="1">MGRDINETRLSHCRDIIKLHRAQTLLRSYSLTESPEASPCGKLPYAVSTLCIEIVIIHHRLALSLFARGTSSYATARCRSTTVPSSTCMHYQPLDQTRQILIKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HDT3</accession>
    <name>A0A9Q1HDT3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">97 kDa heat shock protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_12457</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HDT3"/>
    <dbReference type="OrthoDB" id="434160at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10228">
        <property type="entry name" value="ASKHA_NBD_HSP70_HSPA4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.30:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000171">
        <property type="entry name" value="Heat shock 70 kDa protein 4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000004">
        <property type="entry name" value="Heat shock 70 kDa protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000767">
        <property type="entry name" value="Heat shock protein 70 (HSP70)-4, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45639:SF4">
        <property type="entry name" value="HSC70CB, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45639">
        <property type="entry name" value="HSC70CB, ISOFORM G-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0346">Stress response</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="499"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="669"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="504"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="675"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="714"/>
            <end position="725"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8041593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="725" mass="80920" checksum="FA64A131918ED3B3" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HEL6</accession>
    <name>A0A9Q1HEL6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">Cleavage and polyadenylation specificity factor subunit 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_13321</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the cleavage factor Im (CFIm) complex that functions as an activator of the pre-mRNA 3'-end cleavage and polyadenylation processing required for the maturation of pre-mRNA into functional mRNAs. CFIm contributes to the recruitment of multiprotein complexes on specific sequences on the pre-mRNA 3'-end, so called cleavage and polyadenylation signals (pA signals). Most pre-mRNAs contain multiple pA signals, resulting in alternative cleavage and polyadenylation (APA) producing mRNAs with variable 3'-end formation. The CFIm complex acts as a key regulator of cleavage and polyadenylation site choice during APA through its binding to 5'-UGUA-3' elements localized in the 3'-untranslated region (UTR) for a huge number of pre-mRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer (via N- and C-terminus); binds RNA as homodimer. Component of the cleavage factor Im (CFIm) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the Nudix hydrolase family. CPSF5 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8042301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HEL6"/>
    <dbReference type="OrthoDB" id="277288at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005849">
        <property type="term" value="C:mRNA cleavage factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18871">
        <property type="entry name" value="NUDIX_Cfim25_Nudt21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.79.10:FF:000008">
        <property type="entry name" value="cleavage and polyadenylation specificity factor subunit 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016706">
        <property type="entry name" value="Cleav_polyA_spec_factor_su5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13047">
        <property type="entry name" value="PRE-MRNA CLEAVAGE FACTOR IM, 25KD SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13869">
        <property type="entry name" value="NUDIX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017888">
        <property type="entry name" value="CPSF-25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="9">
        <location>
            <begin position="75"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="17"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR017888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8042301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="226" mass="25821" checksum="A2154D98E1AC32C3" modified="2023-09-13" version="1">MATTPRVSSGQGWPKSSYGQNKYPSATTGVPRERTAVLYPLTNYTFGTKEPLFEKDKSVTARFQRMRDEFPKLGTRRSVEGVLIVHEHGLPHVLLLQLGTTFFKLPGGELKTGEDEVDGLKRLMTEILGRQDGVQQDWMVEDVIANWYRPNFEPPQYPYIPAHITKPKEHKRLYFVQLAEKALFAVPRNYKLVAAPLFELYDNSPGYGPVISSLPQLLSRFNFIYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HEN2</accession>
    <name>A0A9Q1HEN2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Sesquipedalian-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_10648</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HEN2"/>
    <dbReference type="OrthoDB" id="10261837at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055037">
        <property type="term" value="C:recycling endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007032">
        <property type="term" value="P:endosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001881">
        <property type="term" value="P:receptor recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13288">
        <property type="entry name" value="PH_Ses"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045188">
        <property type="entry name" value="Boi1/Boi2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22902:SF53">
        <property type="entry name" value="INOSITOL PHOSPHATASE INTERACTING PROTEIN, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22902">
        <property type="entry name" value="SESQUIPEDALIAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="2">
        <location>
            <begin position="17"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="136"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="143"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="156"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8043529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="432" mass="48370" checksum="E5AC21BB1B3DD13A" modified="2023-09-13" version="1">MRVNHRNVAYFASCNSHVDKEGFLQKFSEHKGTYQLKWFLLKGNILFYFDKKGDREPQGAIVLEGCRIELADTAEGFHAFQLSFSGEASKDYRLVADSPESMESWMKALSCATYDYMKAVVDELQRQMDEISHHMGGEVLQTDDGTQNTDETGSAESHDLPSSEATHSDHARKSRAIVRATFPSESGSTLKSAAFPNPLYDVLDKVPTSPMAPTIEKTTAEKKMPIFVVDRDSTDSESDSEEAGVGGSTDLTYANLNPHFGPGGGSAPQIDFENVTYMSASLEDVSLSSSSTVKGRSLNMTHSESDLPGFLKYAEKETLPVSKSAVQMREQKKIPPQKPPRIQTFFYENMAGIAFGLNNELSAWNQRLSDIPSRASNKFFHREKYPEVVRRDKERSVFHVREVRSFPEMHQDFGVLVIKKVNAFRQRKGQTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HGE1</accession>
    <name>A0A9Q1HGE1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="9">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_11386</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PPP phosphatase family. PP-1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="9"/>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8044041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HGE1"/>
    <dbReference type="OrthoDB" id="1930084at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07414">
        <property type="entry name" value="MPP_PP1_PPKL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000004">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050341">
        <property type="entry name" value="PP1_catalytic_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031675">
        <property type="entry name" value="STPPase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668">
        <property type="entry name" value="SERINE/THREONINE PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668:SF300">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16891">
        <property type="entry name" value="STPPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0464">Manganese</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="11">
        <location>
            <begin position="121"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="307"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8044041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="330" mass="37573" checksum="B441096AB5651B36" modified="2023-09-13" version="1">MAETEKLNVDSIIARLLEVRGARPGKNVQLTEAEIRGLCLKSREIFLSQPNLLELEAPLKICGDIHGQYYDLLRLFEYGGFPPEANYLFLGDYVDRGKQSLETICLLLAYKIKYPENFFLLRGNHECASINRIYGFYDECKRRYNIRLWKTFTDCFNCLPIAAIIDEKIFCCHGGLSPDLVNMEQIRRIMRPTDVPDQGLLCDLLWSDPDKDTLGWGENDRGVSFTFGPEIVSKYLSKHDLDLICRAHQVVEDGYEFFAKRQLVTLFSAPNYCGEFDNAGAMMSVDETLMCSFQILKPADKKKLMYGGVNTGSRPVTPPRGQAKNKSKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HH24</accession>
    <name>A0A9Q1HH24_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_04910</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HH24"/>
    <dbReference type="OrthoDB" id="10257471at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8046289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="173" mass="19886" checksum="872662FA223C8570" modified="2023-09-13" version="1">MNRTLEPKERALTIDIYDVPGRPVCRKCIQPFLSSTSLPVLVFNISDDKSCDAVIDDVNYMRTKLPRTPLILVGLHADRILNEETRRAKCTEIQDAIKEQTKNLIQDLEEELKTFRETTTSGKAETRLIRERIAWIEKRMEELSNETTVIIPASTVTTKVNTRFQESDSSPYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HH29</accession>
    <name>A0A9Q1HH29_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Bifunctional lysine-specific demethylase and histidyl-hydroxylase</fullName>
            <ecNumber evidence="12">1.14.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HOLleu_07560</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Nanhai2018</strain>
            <tissue evidence="15">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Oxygenase that can act as both a histone lysine demethylase and a ribosomal histidine hydroxylase.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="12">Binds 1 Fe(2+) ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ROX family. NO66 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.-" evidence="12"/>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HH29"/>
    <dbReference type="OrthoDB" id="425950at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051864">
        <property type="term" value="F:histone H3K36 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032453">
        <property type="term" value="F:histone H3K4 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.650:FF:000013">
        <property type="entry name" value="Ribosomal oxygenase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1500">
        <property type="entry name" value="JmjC domain-containing ribosomal oxygenase (ROX), dimer domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039994">
        <property type="entry name" value="NO66-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13096">
        <property type="entry name" value="MINA53 MYC INDUCED NUCLEAR ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13096:SF8">
        <property type="entry name" value="RIBOSOMAL OXYGENASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08007">
        <property type="entry name" value="JmjC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="6 12" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="8 12" id="KW-0408">Iron</keyword>
    <keyword evidence="4 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="10 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="9 12" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="JmjC" evidence="14">
        <location>
            <begin position="403"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="48"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="75"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="96"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="122"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="150"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="199"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="287"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU366061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ8044731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="603" mass="66389" checksum="D20C928A0863C233" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HH90</accession>
    <name>A0A9Q1HH90_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Heat shock protein HSP 90-alpha</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_07800</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the heat shock protein 90 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HH90"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001404">
        <property type="entry name" value="Hsp90_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020575">
        <property type="entry name" value="Hsp90_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11528">
        <property type="entry name" value="HEAT SHOCK PROTEIN 90 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00775">
        <property type="entry name" value="HEATSHOCK90"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0346">Stress response</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8044911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="176" mass="19680" checksum="3F47972626A2BCF7" modified="2023-09-13" version="1">MAETESNVTVKERRCTSWRRFEKHIAELISLMDRTSCPGKEVFLRELIFFSTTRLEVREGERMDDSVHNGEELCISVLFDGEENRLTVEDSGAEMTDDYLGTRPKQGTKALISALKAGKDLSNIKLFDVGFFTVFAVADKVTIDSTHSGRTVLYTWESSATEGGLSVKCTPSKLDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HII0</accession>
    <name>A0A9Q1HII0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_04765</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HII0"/>
    <dbReference type="OrthoDB" id="8961218at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="1">
        <location>
            <begin position="9"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8046171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="144" mass="15985" checksum="F7814A6B2757FBDB" modified="2023-09-13" version="1">MTLPSFVPGSALCILAGDFNCVPDTQIDRMSTCTSSGCGVGMSELDMFIKNHDMVDVWRAQHPGLAVFTWHRPDGTDASRLDRVYSPPALSPRDCEIVTCPISDHDAVTVSFQPPESWPTGRGFWKMNTDILTRADYSEQFKAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HJQ4</accession>
    <name>A0A9Q1HJQ4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_02366</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HJQ4"/>
    <dbReference type="OrthoDB" id="5978526at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34615">
        <property type="entry name" value="PX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34615:SF1">
        <property type="entry name" value="PX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8049574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="109" mass="12596" checksum="606FBB8FEF281CC4" modified="2023-09-13" version="1">MITLENGSRVNGLEALCITLQRYAYPCRYGDLLKTYGRPVPHLCMIVKWMTNFIYDNHRHLVSSLEQDWLSPQHLQSFANAIYLKGAALSNCWVFLDGTVRHICRPDQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HKN7</accession>
    <name>A0A9Q1HKN7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">B box-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_01151</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HKN7"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047153">
        <property type="entry name" value="TRIM45/56/19-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25462">
        <property type="entry name" value="BONUS, ISOFORM C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25462:SF296">
        <property type="entry name" value="MEIOTIC P26, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="B box-type" evidence="4">
        <location>
            <begin position="114"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="92"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="168"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="92"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8048721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="596" mass="67147" checksum="42CE2BC6264B0114" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HLC1</accession>
    <name>A0A9Q1HLC1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_02416</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HLC1"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8049606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="174" mass="19278" checksum="9936D1B854A95692" modified="2023-09-13" version="1">MDSRLPEFRVQLPSPFRGQFRFQDLPFPGQFRVQKKAGWTRISISRVCLYNCYSTVDLRGGNHLQLKSSLSIPVLTTLNDLTIEEQGREITSEEFTGILQYSSKCLALKELWFFNCLLPPSVQAESVSVLRSRNVKGNIQYTVSASAPDGVASTSASKVAVSVIADSGIYYSTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0Y9B1</accession>
    <name>A0A9Q0Y9B1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_44945</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010001490">
        <property type="protein sequence ID" value="KAJ8017560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0Y9B1"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000032">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 297-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon gypsy-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF13">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="3">
        <location>
            <begin position="818"/>
            <end position="976"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="287"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="551"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="651"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="39"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="287"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="302"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="326"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="551"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="575"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="595"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="667"/>
            <end position="690"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8017560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1067" mass="121477" checksum="F3BB6BE36B5B0E5F" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YAV8</accession>
    <name>A0A9Q0YAV8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Helitron helicase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_42048</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000025">
        <property type="protein sequence ID" value="KAJ8019387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YAV8"/>
    <dbReference type="OrthoDB" id="10046327at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046700">
        <property type="entry name" value="DUF6570"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025476">
        <property type="entry name" value="Helitron_helicase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051055">
        <property type="entry name" value="PIF1_helicase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47642">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47642:SF5">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20209">
        <property type="entry name" value="DUF6570"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14214">
        <property type="entry name" value="Helitron_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6570" evidence="3">
        <location>
            <begin position="4"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="Helitron helicase-like" evidence="2">
        <location>
            <begin position="244"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="747"/>
            <end position="770"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF20209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8019387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="840" mass="96537" checksum="AF54D8826D663AE5" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YBS0</accession>
    <name>A0A9Q0YBS0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_41395</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8019708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YBS0"/>
    <dbReference type="OrthoDB" id="654211at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF120">
        <property type="entry name" value="KRUPPEL-LIKE FACTOR 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="5">
        <location>
            <begin position="27"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="5">
        <location>
            <begin position="56"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8019708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="226" mass="26617" checksum="8528FA164B1E76C4" modified="2023-09-13" version="1">MAFMCSFCLKHLLHRHLASVHVSEEKHVCKTCQKAFSRGDSLLRHQRQHEGRERCHPCNVCDKSFFRKDNLKSHMKIHVKTKPNQVVLERVKDLTAYISASMVARVAYGWLREISKGWEAGWPSSRDPTCLSFVSMEKLDIGQIDTFQNFVKDEWVFNATEDYLCNTVDIPILKTWLEELRKSWLQSPKRVWDALKEKDIEQTIRTRLEEIFIPPWISKVNYHAVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YBS1</accession>
    <name>A0A9Q0YBS1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_45230</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010002151">
        <property type="protein sequence ID" value="KAJ8017399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YBS1"/>
    <dbReference type="OrthoDB" id="416119at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31635">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31635:SF196">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8017399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="304" mass="35385" checksum="E8F60D10E5B5499C" modified="2023-09-13" version="1">MNRVGGTSDSDKSRNALRELISSFNLLDGYRIINHCNPGLTWFRSNSSSRLDRIYVSRGMKVLRSETIRLPYSDHNPVFVDFEPIIGDSIRKGYWKYNVSLNTDEDFCNDLRFHYQLWKTLKPGFPSLSEWWEEVKNRVKNLAIKHSKRRANEKKEWLKHLQTRCRASQLEDVDSVISDEAEGAFIRSRANYLDLGERPSSFFFRLESRRGNKKVIRSIRGPNGVIYDRKDDICKVFYEYYSSLFSKDKNTDSIAQNDFIKNLNVSIHTHHKEELDLPISIDEVFSALKTASKNKAPGIDGLPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YBU6</accession>
    <name>A0A9Q0YBU6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_42182</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000038">
        <property type="protein sequence ID" value="KAJ8019300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YBU6"/>
    <dbReference type="OrthoDB" id="6141723at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051055">
        <property type="entry name" value="PIF1_helicase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47642">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47642:SF5">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8019300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="64" mass="7561" checksum="6E7317B7C7937352" modified="2023-09-13" version="1">MYIVSYIRKAERDMGDLLRTTQQEAREGNPNAMDELRKLGSMYLHHREVSVMESVYRVTGMHMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YCM7</accession>
    <name>A0A9Q0YCM7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Solute carrier organic anion transporter family member</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_39728</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the organo anion transporter (TC 2.A.60) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YCM7"/>
    <dbReference type="OrthoDB" id="5062115at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015347">
        <property type="term" value="F:sodium-independent organic anion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:monoatomic ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043252">
        <property type="term" value="P:sodium-independent organic anion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17403">
        <property type="entry name" value="MFS_SLCO4_OATP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036058">
        <property type="entry name" value="Kazal_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004156">
        <property type="entry name" value="OATP"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00805">
        <property type="entry name" value="oat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11388">
        <property type="entry name" value="ORGANIC ANION TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11388:SF100">
        <property type="entry name" value="SOLUTE CARRIER ORGANIC ANION TRANSPORTER FAMILY MEMBER 4A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07648">
        <property type="entry name" value="Kazal_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03137">
        <property type="entry name" value="OATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100895">
        <property type="entry name" value="Kazal-type serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51465">
        <property type="entry name" value="KAZAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="78"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="122"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="147"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="202"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="238"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="285"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="357"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="392"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="430"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="543"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="588"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="638"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="80"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="domain" description="Kazal-like" evidence="11">
        <location>
            <begin position="471"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="676"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8020203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="695" mass="74866" checksum="047C3564196B5104" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YCS8</accession>
    <name>A0A9Q0YCS8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">1-phosphatidylinositol-3-phosphate 5-kinase</fullName>
            <ecNumber evidence="3">2.7.1.150</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">HOLleu_41657</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">Nanhai2018</strain>
            <tissue evidence="21">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.150" evidence="3"/>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8019885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="158357at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012506">
        <property type="term" value="C:vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000285">
        <property type="term" value="F:1-phosphatidylinositol-3-phosphate 5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052810">
        <property type="term" value="F:1-phosphatidylinositol-5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032438">
        <property type="term" value="P:melanosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090385">
        <property type="term" value="P:phagosome-lysosome fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046488">
        <property type="term" value="P:phosphatidylinositol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903426">
        <property type="term" value="P:regulation of reactive oxygen species biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03334">
        <property type="entry name" value="Fab1_TCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15725">
        <property type="entry name" value="FYVE_PIKfyve_Fab1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17300">
        <property type="entry name" value="PIPKc_PIKfyve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.810.10:FF:000001">
        <property type="entry name" value="1-phosphatidylinositol 3-phosphate 5-kinase FAB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000206">
        <property type="entry name" value="1-phosphatidylinositol 3-phosphate 5-kinase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000007">
        <property type="entry name" value="1-phosphatidylinositol 3-phosphate 5-kinase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000510">
        <property type="entry name" value="Phosphatidylinositol 3,5-kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.810.10">
        <property type="entry name" value="2-Layer Sandwich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.800.10">
        <property type="entry name" value="Phosphatidylinositol Phosphate Kinase II Beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000591">
        <property type="entry name" value="DEP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043548">
        <property type="entry name" value="PIKfyve"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044769">
        <property type="entry name" value="PIKfyve_PIPKc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027483">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002498">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027484">
        <property type="entry name" value="PInositol-4-P-5-kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46715">
        <property type="entry name" value="1-PHOSPHATIDYLINOSITOL 3-PHOSPHATE 5-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46715:SF1">
        <property type="entry name" value="1-PHOSPHATIDYLINOSITOL 3-PHOSPHATE 5-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00610">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01504">
        <property type="entry name" value="PIP5K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00049">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00330">
        <property type="entry name" value="PIPKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="13 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0967">Endosome</keyword>
    <keyword evidence="10 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FYVE-type" evidence="18">
        <location>
            <begin position="179"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="DEP" evidence="19">
        <location>
            <begin position="340"/>
            <end position="416"/>
        </location>
    </feature>
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        <location>
            <begin position="1844"/>
            <end position="2168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="261"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="509"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="853"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="885"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="930"/>
            <end position="973"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1540"/>
            <end position="1709"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1789"/>
            <end position="1826"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1838"/>
            <end position="1857"/>
        </location>
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        <location>
            <begin position="1480"/>
            <end position="1507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="38"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="59"/>
            <end position="77"/>
        </location>
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        <location>
            <begin position="509"/>
            <end position="519"/>
        </location>
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        <location>
            <begin position="948"/>
            <end position="973"/>
        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1540"/>
            <end position="1565"/>
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            <begin position="1582"/>
            <end position="1593"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1645"/>
            <end position="1665"/>
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        <location>
            <begin position="1674"/>
            <end position="1707"/>
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        <location>
            <begin position="1790"/>
            <end position="1807"/>
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        <location>
            <begin position="1809"/>
            <end position="1820"/>
        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00012009"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
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            <dbReference type="ARBA" id="ARBA00022771"/>
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            <dbReference type="ARBA" id="ARBA00022777"/>
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            <dbReference type="ARBA" id="ARBA00022786"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
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            <dbReference type="PROSITE-ProRule" id="PRU00781"/>
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        <source>
            <dbReference type="SAM" id="Coils"/>
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    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50186"/>
        </source>
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        <source>
            <dbReference type="PROSITE" id="PS51455"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAJ8019885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="2187" mass="245887" checksum="EED5333FA390D49B" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YD56</accession>
    <name>A0A9Q0YD56_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Chloride channel protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_44534</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000972">
        <property type="protein sequence ID" value="KAJ8017817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Cl_channel_volt-gated"/>
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        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN 2"/>
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    </dbReference>
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        <location>
            <begin position="98"/>
            <end position="121"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAJ8017817.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="121" mass="13572" checksum="E3862028A3F4A429" modified="2023-09-13" version="1">MVKNVKFISWLSTYKDLKDLLDGSGHSSYPLVDSPENMVLLGSMQRGELEYLMEKKIGRARRLIVVAERKMRERGAEEEDREMRAMGQNATIVSFHRQSEGNSEGGGITISPVADGKLTLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YDK9</accession>
    <name>A0A9Q0YDK9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Adhesion G-protein coupled receptor G4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HOLleu_39921</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
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            <taxon>Eukaryota</taxon>
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            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Nanhai2018</strain>
            <tissue evidence="17">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="KAJ8020350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome 22"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="7tmB2_Adhesion"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.220.50">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038081">
        <property type="entry name" value="CalX-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003644">
        <property type="entry name" value="Calx_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057244">
        <property type="entry name" value="GAIN_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046338">
        <property type="entry name" value="GAIN_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000203">
        <property type="entry name" value="GPS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003410">
        <property type="entry name" value="HYR_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45692:SF1">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 2 PROFILE 2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45692">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Calx-beta"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01825">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00237">
        <property type="entry name" value="Calx_beta"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00303">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141072">
        <property type="entry name" value="CalX-like"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50221">
        <property type="entry name" value="GAIN_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50825">
        <property type="entry name" value="HYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
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    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="2392"/>
            <end position="2412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
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            <begin position="2424"/>
            <end position="2445"/>
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            <begin position="2465"/>
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            <begin position="2591"/>
            <end position="2615"/>
        </location>
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        <location>
            <begin position="2621"/>
            <end position="2644"/>
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        <location>
            <begin position="247"/>
            <end position="286"/>
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        <location>
            <begin position="1615"/>
            <end position="1701"/>
        </location>
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        <location>
            <begin position="1922"/>
            <end position="1960"/>
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        <location>
            <begin position="2186"/>
            <end position="2378"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="15">
        <location>
            <begin position="2388"/>
            <end position="2646"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="276"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="1931"/>
            <end position="1948"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="1950"/>
            <end position="1959"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
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    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50221"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ8020350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="2712" mass="300295" checksum="6A002E1AA91A0785" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YEP4</accession>
    <name>A0A9Q0YEP4_HOLLE</name>
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        <submittedName>
            <fullName evidence="4">Segment polarity protein dishevelled-like DVL-3</fullName>
        </submittedName>
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        <name evidence="4" type="ORF">HOLleu_38530</name>
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    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
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            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
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    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="OrthoDB" id="10031689at2759"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:canonical Wnt signaling pathway"/>
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        <property type="project" value="InterPro"/>
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        <location>
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        </location>
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            <begin position="492"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8021358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="562" mass="61413" checksum="1296F22E4702F1D9" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YF30</accession>
    <name>A0A9Q0YF30_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_42861</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000150">
        <property type="protein sequence ID" value="KAJ8018897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YF30"/>
    <dbReference type="OrthoDB" id="6417614at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8018897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="106" mass="12211" checksum="9CA3C73C03F46A03" modified="2023-09-13" version="1">MSRRVTEQAPFLHVLTRGTTQQRSALLKRLHNALLICLCECALNILKGNVKLTPSEKLHLQRHRAKLRKLVDRKVSLSQKRKVFRQKGGAHCVRSMLLPIIKQLQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YG48</accession>
    <name>A0A9Q0YG48_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein ALP1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_39524</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8022117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YG48"/>
    <dbReference type="OrthoDB" id="1681765at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027806">
        <property type="entry name" value="HARBI1_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13359">
        <property type="entry name" value="DDE_Tnp_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE Tnp4" evidence="3">
        <location>
            <begin position="2"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8022117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="211" mass="23619" checksum="5278A9EE43CAF245" modified="2023-09-13" version="1">MAVVGADYEFIMVDAGVNGRVSDGGVIAVTEFGRLLDDGSLGLPEPAPLHQNDVTAMPYVFVGDDAFAMSENLLKPYGGDRLESDQRIYNYRLSRARRVTENAFGILTARFGVFQKAMQLSPEKATLITMTCCYLHNFLRKKSRCYIGPGAVDWEDANHEMHAGEWRASQRQLEGLRATMNRNAGNTARLVRDAFRHYFCTQGQVPWQDGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YHH7</accession>
    <name>A0A9Q0YHH7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_37661</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YHH7"/>
    <dbReference type="OrthoDB" id="6022531at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008528">
        <property type="term" value="F:G protein-coupled peptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009755">
        <property type="term" value="P:hormone-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24372:SF77">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24372">
        <property type="entry name" value="GLYCOPROTEIN HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="75"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="120"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="164"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="207"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="282"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="324"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="361"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="9">
        <location>
            <begin position="94"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8022693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="407" mass="45028" checksum="A72C77E2E8083ADA" modified="2023-09-13" version="1">MAGRCPGGCSFCSHGILECINFKPLTAEDVEYMRQTLHVTVIEAVSDDYSICCIINTGTECVAPPSPFATCDGIFYIWVLRVSAWIVSLAALVTNLIVLIGRLKTRQLDATNAKPTENIFLVNLAVADFLMGAYLLAIAITDAWFGSDYFLYSNTWRTGPTCGIIGVIGVLSSVVSLLDLTIITIDRFFCIVFPFGKIHFNPKWGKISCSIIWMIGIAIAILPLFLSHSVQNFYGYTDVCLGLPIVAIPQKVSQSLVERGNIILHVGYTFEAIPTWVYSAVIYTYLSSVCLFIVTACYIYMFISVLKTRQDSHRASGSNDEVKLATRISIIVGTDMLCWLPVIILSVLSQFRVQIPSKTNQWLVVFVIPINSALNPFIFSYGIIKARKTSAPGQSQEMREKPKGCFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YI40</accession>
    <name>A0A9Q0YI40_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_40319</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YI40"/>
    <dbReference type="OrthoDB" id="10678024at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.2000.10">
        <property type="entry name" value="Frizzled cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036790">
        <property type="entry name" value="Frizzled_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63501">
        <property type="entry name" value="Frizzled cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="122"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="309"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="472"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="122"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="158"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8020667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="639" mass="67786" checksum="2F0C8EA24E1E4DBD" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YMG6</accession>
    <name>A0A9Q0YMG6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Proteasome activator complex subunit 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_35195</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PA28 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8025087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YMG6"/>
    <dbReference type="OrthoDB" id="6591885at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008537">
        <property type="term" value="C:proteasome activator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061133">
        <property type="term" value="F:endopeptidase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000045">
        <property type="term" value="P:regulation of G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061136">
        <property type="term" value="P:regulation of proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.180:FF:000001">
        <property type="entry name" value="Proteasome activator complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.180">
        <property type="entry name" value="Proteasome activator pa28, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.120">
        <property type="entry name" value="Proteasome activator pa28, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003186">
        <property type="entry name" value="PA28_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036997">
        <property type="entry name" value="PA28_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036996">
        <property type="entry name" value="PA28_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009077">
        <property type="entry name" value="Proteasome_activ_PA28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003185">
        <property type="entry name" value="Proteasome_activ_PA28_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036252">
        <property type="entry name" value="Proteasome_activ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10660:SF2">
        <property type="entry name" value="LD45860P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10660">
        <property type="entry name" value="PROTEASOME REGULATOR PA28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02252">
        <property type="entry name" value="PA28_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02251">
        <property type="entry name" value="PA28_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47216">
        <property type="entry name" value="Proteasome activator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0647">Proteasome</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome activator PA28 N-terminal" evidence="3">
        <location>
            <begin position="11"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="Proteasome activator PA28 C-terminal" evidence="4">
        <location>
            <begin position="100"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8025087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="245" mass="28145" checksum="EEFE422197C473D0" modified="2023-09-13" version="1">MDKSDNETNLQAVEAVKKHILEKGEALVQDVFPAKVLELNKLAESDIFKMDRMQVIQKNVDNIPVPQPVITNHVSENFKKRKLDSDIEGSPVLVFPGGTIPCNKDIIHITNQLKPLICVLIEHCNSLKMWIQLLIPRIEDGNNFGVSIQEDTLAELRQVESDAAAYLDQITRYFITRGKLISKVAKYPHVEDYRKSVEEIDQKEFFNLRLTCLELRNTYLSLLDLIEKNKDKIKKPRNSNAETLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YMZ3</accession>
    <name>A0A9Q0YMZ3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Twist-related protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_34302</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YMZ3"/>
    <dbReference type="OrthoDB" id="8583783at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000030">
        <property type="entry name" value="Twist transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050283">
        <property type="entry name" value="E-box_TF_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23349">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, TWIST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23349:SF50">
        <property type="entry name" value="PROTEIN TWIST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="2" id="KW-0221">Differentiation</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="8">
        <location>
            <begin position="218"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="128"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="12"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="132"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="197"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8024401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="315" mass="35667" checksum="ADEF776C6A87398F" modified="2023-09-13" version="1">MENHRVPYSEGNWGNSPPQNGSIAVCRNDNENSDLHGASWRGSVVFDSSHSQRAWRPNVAGSPAEYYHRNPQHWSRVSHLPTFHNRSSCAFAESENNCIAPNKSLNFNGWNIQTTSDSVQHCTRVKMEPTDFSPSSEGSFSDTLSLTGSDMKRDPELSPGAPGAYEDEDMRKNRKRKYRSSDGEDTESLTGEDTSSKRRGRGGKCRRKGPLNFDEVQNQRCMANVRERQRTQNLNDAFSNLRKIIPTLPSDKLSKIQTLKLASRYIDFLYQVLGSDEKDQKMAGSCSYMAHERLSYAFSVWRMEGAWSSMSSSER</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YN21</accession>
    <name>A0A9Q0YN21_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_33069</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YN21"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8025494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="104" mass="11344" checksum="18172EBBA58FACE7" modified="2023-09-13" version="1">MVYTAVRRNNALPKLSEPGEFGTPVSVVLSEGKGKMETFWLEGRKDPANHLNSAEDSSYADHSTVNISGPNDANVTQVTEVIAVEEEENLPYVTKLGYGYQVAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YNR7</accession>
    <name>A0A9Q0YNR7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Hexosyltransferase</fullName>
            <ecNumber evidence="9">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_34666</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Golgi apparatus</location>
            <location evidence="1 9">Golgi stack membrane</location>
            <topology evidence="1 9">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the chondroitin N-acetylgalactosaminyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="9"/>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YNR7"/>
    <dbReference type="OrthoDB" id="9985088at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047238">
        <property type="term" value="F:glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008428">
        <property type="entry name" value="Chond_GalNAc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051227">
        <property type="entry name" value="CS_glycosyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12369">
        <property type="entry name" value="CHONDROITIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12369:SF13">
        <property type="entry name" value="HEXOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05679">
        <property type="entry name" value="CHGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8024690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="789" mass="90341" checksum="BC960E213DE09C35" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YR70</accession>
    <name>A0A9Q0YR70_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Pancreatic lipase-related protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_34039</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8026246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YR70"/>
    <dbReference type="OrthoDB" id="199913at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016298">
        <property type="term" value="F:lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00707">
        <property type="entry name" value="Pancreat_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013818">
        <property type="entry name" value="Lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033906">
        <property type="entry name" value="Lipase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000734">
        <property type="entry name" value="TAG_lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610">
        <property type="entry name" value="LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610:SF178">
        <property type="entry name" value="LIPASE MEMBER H-A-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00151">
        <property type="entry name" value="Lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00821">
        <property type="entry name" value="TAGLIPASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="domain" description="Lipase" evidence="6">
        <location>
            <begin position="36"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8026246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="369" mass="40525" checksum="2FAFD1FFAB4A8712" modified="2023-09-13" version="1">MEFMYSKCGAFSSYLVAGIIAGFILQMFFVSVEGQEVCYPDVGCFDSTLECNYHFLPQSPGEINTMFFLYTPQNPSTSVTLNWQDDSSIINSNFRGNRGTKFIIHGFIGNSMRGYVTAMKDALLSIGNFNVILVNWENGADLFDPIQMYTDVINLPFGYEIARQNARLVGRQIGLLSQKLQQLTGASYADMHLIGHSLGAQTAGFAGEFLSGQYGRITGLDPAGPGFDTFRNSRGCLLDSSDAIFVDIIHTDSTLGNEYATGHQDFYPNGGVYQVGCVPLDEACDHNRAIYYFTESITTTCEFLAYPCSSWTNFLKGKCRSCGSSGCSRMGYYADLSTATGSFRLGTNTGSPFCKGDSGQPPKFIDFFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YRV3</accession>
    <name>A0A9Q0YRV3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Cuticle-degrading protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_34137</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the peptidase S8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8026325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YRV3"/>
    <dbReference type="OrthoDB" id="206201at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04077">
        <property type="entry name" value="Peptidases_S8_PCSK9_ProteinaseK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000014">
        <property type="entry name" value="Proteinase K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.80">
        <property type="entry name" value="Peptidase S8 propeptide/proteinase inhibitor I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034193">
        <property type="entry name" value="PCSK9_ProteinaseK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050131">
        <property type="entry name" value="Peptidase_S8_subtilisin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037045">
        <property type="entry name" value="S8pro/Inhibitor_I9_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806:SF58">
        <property type="entry name" value="ALKALINE PROTEASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806">
        <property type="entry name" value="PEPTIDASE S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 6 7" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6 7" id="KW-0720">Serine protease</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040394641" evidence="8">
        <location>
            <begin position="20"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="9">
        <location>
            <begin position="130"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5 6">
        <location>
            <position position="139"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5 6">
        <location>
            <position position="173"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5 6">
        <location>
            <position position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8026325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="379" mass="40947" checksum="88804AEC3DB2CEBF" modified="2023-09-13" version="1">MRNSLLCCVFTTLVVTSSAIAPFYRNEEPVKNSYIIVLKDDFDPDDIVNTVSARAQVSAMNFQRKFTFRRILNGFTAELTEDLLPFIRSLDAVKYVEENGVAREDETWGLDRIDQRYLPLDDEYKPIGDGAGVNVYVLDTGIRTTHEEFEDRASFVFDAMEYAIGDDSDCRGHGTHCAGTIAGKVYGVAKKARVNAVRVLGCQGSGTWAAIINGMEWVAQNGIRPAIASMSLGGGVTRAVNEGVAGMFNAGILSTVSAGNNDFDSCQKSPASTPEAITVGASQSDDIRAYFSNYGACVDIFAPGRSITAAYYRADDDYAILSGTSMSTPHVAGVAAIHLGLNPELTPAELRDIILNSATRDPIKDVKSSPGLFLYVPRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YSE4</accession>
    <name>A0A9Q0YSE4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_33283</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YSE4"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040170205" evidence="2">
        <location>
            <begin position="20"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8025662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="57" mass="6471" checksum="F273EEFB1DCF0B85" modified="2023-09-13" version="1">MKRETYAVMMACVCMCVCACVCVCVCVCVFVTLCSLTRYLKKGNSDCFQTWYIDAPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BAQ5</accession>
    <name>A0A9Q1BAQ5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Deleted in malignant brain tumors 1 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_43503</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000336">
        <property type="protein sequence ID" value="KAJ8018483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BAQ5"/>
    <dbReference type="OrthoDB" id="536948at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000006">
        <property type="entry name" value="neurotrypsin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48071:SF18">
        <property type="entry name" value="DELETED IN MALIGNANT BRAIN TUMORS 1 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48071">
        <property type="entry name" value="SRCR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00530">
        <property type="entry name" value="SRCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00258">
        <property type="entry name" value="SPERACTRCPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00420">
        <property type="entry name" value="SRCR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="SRCR" evidence="9">
        <location>
            <begin position="35"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="7">
        <location>
            <begin position="140"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="8">
        <location>
            <begin position="282"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="59"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="72"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="103"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="206"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8018483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="371" mass="42304" checksum="8DD1F77C38C95418" modified="2023-09-13" version="1">MMEAVSLDTGNVMVWRSVMEEKMNYGVTEQDSTQIRIVGNNEREGRVEVYHNGQWGTVCDDSWDDDDATVVCRQLGLGESGTAVCCASFGAGEGPIWLDDVRCTGSESNIGSCGNNGWGIEDCGHLEDAGVRCWTETPFCGTVPFNDFLRRRNYTCTAPSAYIASANYPLPYEQNRDWFWHITTSPSTYIEVKFEEFDIESSTKDCEEDYLEFQMRSFILRVCNSRNNFFNSSFLSDQNNLTVKLHSGRFSFGGRFLAIYWERKFVQNVAVQQNVTCRVQNGRRHCYCLFNYHQALTWQNASQGCKNCGDGGYLAVIQSQQDMDTLQQFILQEGDAYQSAYIGLKWNEDSQRFVLVSQASLHLFTDWKVSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BB23</accession>
    <name>A0A9Q1BB23_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Collectin-12</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_41958</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8020105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BB23"/>
    <dbReference type="OrthoDB" id="2142683at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C-type lectin" evidence="1">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8020105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="103" mass="11743" checksum="0039C02C540CF2C5" modified="2023-09-13" version="1">MSSHLVFIESYEEQQFLSETYNSSHAYWLGLNGDSYSEKTWDDGSVMVYSRIGSHATATDNNSGCYRIITDAQAIKWEDEAGSWFDKSCDARYGYICEYEMGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BB39</accession>
    <name>A0A9Q1BB39_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_43382</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000289">
        <property type="protein sequence ID" value="KAJ8018559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BB39"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8018559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="95" mass="11084" checksum="9FBA7B9080CC962C" modified="2023-09-13" version="1">MLGYRYVYAGKYILFFIFVRNSSISASFCITNISQTISILTNPSQTIKFFARFKPYVSVILIINIFRWQLSEKRNSKKLKVYELTGKMLAYDSGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BCE4</accession>
    <name>A0A9Q1BCE4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 17">Exonuclease 1</fullName>
            <ecNumber evidence="17">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">HOLleu_41042</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">Nanhai2018</strain>
            <tissue evidence="21">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">5'-&gt;3' double-stranded DNA exonuclease which may also contain a cryptic 3'-&gt;5' double-stranded DNA exonuclease activity. Also exhibits endonuclease activity against 5'-overhanging flap structures similar to those generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Required for DNA mismatch repair (MMR).</text>
    </comment>
    <comment type="function">
        <text evidence="17">5'-&gt;3' double-stranded DNA exonuclease which may also possess a cryptic 3'-&gt;5' double-stranded DNA exonuclease activity. Functions in DNA mismatch repair.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="17">Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 17">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the XPG/RAD2 endonuclease family. EXO1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="17"/>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8019442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BCE4"/>
    <dbReference type="OrthoDB" id="26491at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035312">
        <property type="term" value="F:5'-3' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017108">
        <property type="term" value="F:5'-flap endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09908">
        <property type="entry name" value="H3TH_EXO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09857">
        <property type="entry name" value="PIN_EXO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000011">
        <property type="entry name" value="exonuclease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000096">
        <property type="entry name" value="Exonuclease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036279">
        <property type="entry name" value="5-3_exonuclease_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037315">
        <property type="entry name" value="EXO1_H3TH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008918">
        <property type="entry name" value="HhH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044752">
        <property type="entry name" value="PIN-like_EXO1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006086">
        <property type="entry name" value="XPG-I_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006084">
        <property type="entry name" value="XPG/Rad2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019974">
        <property type="entry name" value="XPG_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006085">
        <property type="entry name" value="XPG_DNA_repair_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081:SF8">
        <property type="entry name" value="EXONUCLEASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081">
        <property type="entry name" value="FLAP ENDONUCLEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00867">
        <property type="entry name" value="XPG_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00752">
        <property type="entry name" value="XPG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00853">
        <property type="entry name" value="XPGRADSUPER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00279">
        <property type="entry name" value="HhH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00484">
        <property type="entry name" value="XPGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00485">
        <property type="entry name" value="XPGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47807">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00841">
        <property type="entry name" value="XPG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 17" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8 17" id="KW-0228">DNA excision</keyword>
    <keyword evidence="14 17" id="KW-0234">DNA repair</keyword>
    <keyword evidence="13 17" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="12 17" id="KW-0267">Excision nuclease</keyword>
    <keyword evidence="10 17 21" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="9 17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11 17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 17" id="KW-0540">Nuclease</keyword>
    <keyword evidence="15 17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="XPG N-terminal" evidence="20">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="XPG-I" evidence="19">
        <location>
            <begin position="138"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="382"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="443"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="497"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="603"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="654"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="741"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="881"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="382"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="505"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="628"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="662"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="679"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="702"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="741"/>
            <end position="758"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00055562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU910737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAJ8019442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1017" mass="113871" checksum="B703349E3C89CDD1" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BD09</accession>
    <name>A0A9Q1BD09_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">Adenosine kinase</fullName>
            <shortName evidence="11">AK</shortName>
            <ecNumber evidence="3 11">2.7.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Adenosine 5'-phosphotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_38306</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">ATP dependent phosphorylation of adenosine and other related nucleoside analogs to monophosphate derivatives.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>adenosine + ATP = AMP + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20824"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16335"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="11">Binds 3 Mg(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenosine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the carbohydrate kinase PfkB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.20" evidence="3 11"/>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8023196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BD09"/>
    <dbReference type="OrthoDB" id="432447at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004001">
        <property type="term" value="F:adenosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006169">
        <property type="term" value="P:adenosine salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044209">
        <property type="term" value="P:AMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006144">
        <property type="term" value="P:purine nucleobase metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01168">
        <property type="entry name" value="adenosine_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1190.20:FF:000006">
        <property type="entry name" value="Adenosine kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1110.10:FF:000001">
        <property type="entry name" value="Adenosine kinase a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001805">
        <property type="entry name" value="Adenokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002173">
        <property type="entry name" value="Carboh/pur_kinase_PfkB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45769">
        <property type="entry name" value="ADENOSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45769:SF3">
        <property type="entry name" value="ADENOSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00989">
        <property type="entry name" value="ADENOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00584">
        <property type="entry name" value="PFKB_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 11 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 11" id="KW-0660">Purine salvage</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbohydrate kinase PfkB" evidence="12">
        <location>
            <begin position="40"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR601805-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU368116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8023196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="357" mass="39476" checksum="159E85FFE58ED183" modified="2023-09-13" version="1">MSTHDNGEPGIKKAKMATSESSIVAIGNPLLDISATGTKKFLEKYGLDANNSILAEEKHIPMFQEMVDNFNVEYIPGGATQNTIRMAQWVLQEPKRCAFFGCIGNDEYGKILSNKMLEFGAHVNYLINEDHPTGTCACVLTGNDRSLVANLAAANHYKKDHLMKPENWEHVVKAKLLYVAGFHLTVATDAMLELGKHASKHNKIFSTNLSAPFLCQFFSEQQMSVMPYVDFLFGNETEFAAFSKQQNFETEDLTEIALKAAALPKINKQRERTVVITQGSLPTIVAHGGKVTEYPVNEVKEEDIVDTNGAGDCFVGGFLSQLIQDKSIEDCIKVANYCASYIIKQSGITMEDKPCIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BEQ6</accession>
    <name>A0A9Q1BEQ6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_37727</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BEQ6"/>
    <dbReference type="OrthoDB" id="6022531at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008528">
        <property type="term" value="F:G protein-coupled peptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009755">
        <property type="term" value="P:hormone-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24372:SF77">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24372">
        <property type="entry name" value="GLYCOPROTEIN HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="61"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="107"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="179"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="227"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="264"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="8">
        <location>
            <begin position="1"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8022743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="350" mass="38864" checksum="4936473518AF5E95" modified="2023-09-13" version="1">MVLIRRWKTKPNATTAKPTENIFLANLAVADFLMGVYLLAIAKADAAFGSDYYLYSKTWRTGPTCGIIGVIGVLSSVVSLLALTFMTIDRLLCIVFTFGKIHFNSKWGKIICSIIWVIGFAIAIPPSFLSHSLQNFYGYSDVCLGLPIAAIPQKVTGVTEMREGVMHHVGYTSGAMSTWLYSAILYVYLSSICLFIVIICYVSMFISVLRSGQHSNRAAGSSDDLKLATRMSIIVGTDMLCWLPVIILGVLSQCGKQIPSKANPWLVVFVVPINSALNPFIYSYGILKKRKKNNPNYIQEDSEVAPQRKRTTYPFHQLYVSNSNTEDMLEGLSKDRRYNTTQSDQSVGAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BFT2</accession>
    <name>A0A9Q1BFT2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="1">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_36617</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="1"/>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8024018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BFT2"/>
    <dbReference type="OrthoDB" id="9993594at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030054">
        <property type="term" value="C:cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14547">
        <property type="entry name" value="PTPc-KIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000020">
        <property type="entry name" value="Tyrosine-protein phosphatase non-receptor type 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008356">
        <property type="entry name" value="Tyr_Pase_KIM-con"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46198">
        <property type="entry name" value="PROTEIN-TYROSINE-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46198:SF4">
        <property type="entry name" value="PROTEIN-TYROSINE-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01778">
        <property type="entry name" value="KIMPTPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="60"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="8">
        <location>
            <begin position="217"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="9">
        <location>
            <begin position="366"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate" evidence="5">
        <location>
            <position position="393"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="358"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="393"/>
            <end position="399"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="437"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR608356-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR608356-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8024018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="461" mass="52662" checksum="18A41072FB9D5EB4" modified="2023-09-13" version="1">MRARTVSLPLNDHVTQESGSLQGTISMVPGHHSTETTTVAPDIHKIPTGQNLWQSSHAPYLVGVFCAIFIIFIIIVCYCCYRAWRSKHLRDEEKRRIALFPSIKPETREGSQIQPEGSYIQYASIGRTQPITIQTREPGLQERRSSSTSLTLEISGPSDTVRWVASPPKESSVEEYLESAGNRLTRKQLRNALKNTRALHEEFWAIPMNHPETIEVPGSTAKNRYRTILPNPSTRVVLPLIPGDSLSDYINANRIRGYDPEKFDFIACQGPMTHTIADFWRLVWHSNSPIIVMVTKLKERNRSKCEMYWPQRQGFYGEIEVTVEDIIKQDDYILRIFAVKYLEESRQVLHYWYTSWPDNKPPENPNTLLNMIKDVEISRKDTSLTDGPVIVHCSAGLGRTGCYIAISIGMRQLDEEHMTDILGIVCQIRQDRGGLVQTNEQYEFIHQALCLHEKSLPETPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BFW3</accession>
    <name>A0A9Q1BFW3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein quiver</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_35153</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8025055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BFW3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040391974" description="Protein quiver" evidence="2">
        <location>
            <begin position="22"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="147"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8025055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="166" mass="17940" checksum="FB4AC5A6200D2CA3" modified="2023-09-13" version="1">MALTGTSFLAICMALLLTVHGLKCYDKTENKCTGNERICDTLSSDKELTEATCDDGVTMCSLMTFKKAAGLAALGAGYEIETVKAECLPAETDTTLTSDCYGREKLRDINPDEEDVLTSYERNPLNTVKSLEICICKGDLCNGSIKSVFSISVIISSVVMSFVLFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BHB3</accession>
    <name>A0A9Q1BHB3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_35364</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8025219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BHB3"/>
    <dbReference type="OrthoDB" id="29460at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018939">
        <property type="entry name" value="Autophagy-rel_prot_27"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09451">
        <property type="entry name" value="ATG27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="32"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8025219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="106" mass="11793" checksum="D38DEA1673BD270B" modified="2023-09-13" version="1">MLMIYLLRDLSRNDLKQIDVNTGILQTNATQMSTFSVLLVVYIASGIGYAKYKGRTGKEMIPNYSFWSDLPYAIKDGFKFSISLVRGKSYTPSTSAKAKPSAYGSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BHJ5</accession>
    <name>A0A9Q1BHJ5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ectonucleotide pyrophosphatase/phosphodiesterase family member 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_31845</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BHJ5"/>
    <dbReference type="OrthoDB" id="415411at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd16018">
        <property type="entry name" value="Enpp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002591">
        <property type="entry name" value="Phosphodiest/P_Trfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10151:SF120">
        <property type="entry name" value="BIS(5'-ADENOSYL)-TRIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10151">
        <property type="entry name" value="ECTONUCLEOTIDE PYROPHOSPHATASE/PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01663">
        <property type="entry name" value="Phosphodiest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040390678" evidence="1">
        <location>
            <begin position="22"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8026885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="470" mass="53210" checksum="DECD7FE6B9263077" modified="2023-09-13" version="1">MLPGCFSCTLLVICVWTATSAGEHMKGSLLLVVGMDSFRWDFLNRAQTPNFDYIIENGVRAQHLVNVFPTKSAPNWYTILTGLYPESHGLVNNHVYEPKIDPDKPIIAIVSQSEDPILWGNAEPIWIINQRQGGVSGVLNFPFAMTVSYNGMVANFTTNTYINDTTNEERVDMIVELFASGAINFGMIYYHEVDHACHKYGPESPEVDGVIEEMDAVVGYLVKKLKESNLYDVINIMFVADHGQRKMYPKQTVFLNEYIDPSLYLGFQDNPVYSIFPHNVSQTQDIVNNLTRANNLTVCTKDTIPEKYHYRNNDRIPPILLVADKGWNVAQNKSEEPYWEKTIGEHGYGNDDPKMWPFFVARGPAFKKGHTHAEPFNTVDLYSIMCHIMKLEPAPHNGSLENVKHVLADYKTPEQPSVLRGIIDLFIIGVVIETCILLATCSKLHRYKVQVKKMKRSAIEEEGLLDSPES</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BHW5</accession>
    <name>A0A9Q1BHW5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Organic cation transporter protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_31672</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BHW5"/>
    <dbReference type="OrthoDB" id="3936150at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17317">
        <property type="entry name" value="MFS_SLC22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24064">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="158"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="184"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="209"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="242"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="477"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="506"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="539"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="562"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="593"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="621"/>
            <end position="641"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8026748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="722" mass="81258" checksum="94682EFAD0B13EE4" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BI89</accession>
    <name>A0A9Q1BI89_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Deleted in malignant brain tumors 1 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_31500</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BI89"/>
    <dbReference type="OrthoDB" id="536948at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000006">
        <property type="entry name" value="neurotrypsin isoform X2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000011">
        <property type="entry name" value="Scavenger receptor class A member 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48071">
        <property type="entry name" value="SRCR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48071:SF28">
        <property type="entry name" value="SRCR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00530">
        <property type="entry name" value="SRCR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00258">
        <property type="entry name" value="SPERACTRCPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00420">
        <property type="entry name" value="SRCR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 8" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040113582" evidence="8">
        <location>
            <begin position="24"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="698"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="9">
        <location>
            <begin position="29"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="9">
        <location>
            <begin position="144"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="9">
        <location>
            <begin position="260"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="9">
        <location>
            <begin position="372"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="9">
        <location>
            <begin position="484"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="9">
        <location>
            <begin position="592"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="743"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="829"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="749"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="840"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="906"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="53"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="66"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="97"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="169"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="182"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="213"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="285"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="298"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="329"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="440"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="554"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="617"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="661"/>
            <end position="671"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8026614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="915" mass="101343" checksum="A50C957612B2C669" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BIA3</accession>
    <name>A0A9Q1BIA3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CDK2-associated and cullin domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_34103</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cullin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8026297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BIA3"/>
    <dbReference type="OrthoDB" id="8172509at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1310.10">
        <property type="entry name" value="Cullin Repeats"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042652">
        <property type="entry name" value="CACUL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001373">
        <property type="entry name" value="Cullin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016159">
        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46636">
        <property type="entry name" value="CDK2-ASSOCIATED AND CULLIN DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46636:SF1">
        <property type="entry name" value="CDK2-ASSOCIATED AND CULLIN DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00888">
        <property type="entry name" value="Cullin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cullin N-terminal" evidence="2">
        <location>
            <begin position="20"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8026297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="243" mass="28059" checksum="64C1F1943C8B9833" modified="2023-09-13" version="1">MECSNAMASLDETDYETKLWPPLENAVNMLLTQQPGVYLKISYEEMYRYVYTSVCHQHSARIYEDLVNLLSENLSNFSQKLENLHGVTLLDQFHSGLKRYTQAIGCIVPIFAYLNRTYIMMQMKSDLKKELTALLNKTVIDHHVLRVLDELSAAQSKPFGCSPAVAADLVRNLNDFRPDFARIYPHLFAKYIPNILPPARADELPLYIEETHRMQQELVTSDDFRRCDQSKKRALDDTPVVPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BJ31</accession>
    <name>A0A9Q1BJ31_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Tubulin alpha chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HOLleu_32715</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Nanhai2018</strain>
            <tissue evidence="16">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="13">Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the tubulin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BJ31"/>
    <dbReference type="OrthoDB" id="6073114at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02186">
        <property type="entry name" value="alpha_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.600:FF:000005">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.20:FF:000001">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000007">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.600">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002452">
        <property type="entry name" value="Alpha_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01162">
        <property type="entry name" value="ALPHATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10 13" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 13" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="14">
        <location>
            <begin position="34"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="15">
        <location>
            <begin position="233"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ8027547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="437" mass="49163" checksum="EDBF48C97DE7FA66" modified="2023-09-13" version="1">MGQSCWELYCLEHGIRPDGTMFGNMSPDKECDAYNTFFSETGKGKHVPRAIFVDLEPTVVDVIRLGDYRQLFHPEQMITGTEDAANNYARGHYTVGREIIDKVLDRIRKLSEQCVGLQGFLVFHSFGGGTGSGFNALLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNAILTTHTTLEHTDCSFMVDNEAIYDLCRRNLDIDRPSYENLNRLIAQVVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYSPIISAEKAYHEQLTVAEITNACFEPANQMVKCDPRHGKYMSCCLLYRGDVVPKDVNEAVATIKTRRNIQFVDWCPTGFKVGINYQPPTVIPSGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFTEAREDMAALEKDYEEVGIDSADACAESSDEEEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-04-02" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BK94</accession>
    <name>A0A9Q1BK94_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Gem-associated protein 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_30363</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="7326421at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032797">
        <property type="term" value="C:SMN complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000387">
        <property type="term" value="P:spliceosomal snRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056424">
        <property type="entry name" value="Beta-prop_GEMI5_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056420">
        <property type="entry name" value="GEMI5_RBS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052640">
        <property type="entry name" value="Gemin-5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056421">
        <property type="entry name" value="TPR_GEMI5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46362">
        <property type="entry name" value="GEM-ASSOCIATED PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46362:SF1">
        <property type="entry name" value="GEM-ASSOCIATED PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23775">
        <property type="entry name" value="Beta-prop_RIG_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23777">
        <property type="entry name" value="GEMI5_RBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23774">
        <property type="entry name" value="TPR_GEMI5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="50"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="214"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="Gem-associated protein 5 second beta-propeller" evidence="6">
        <location>
            <begin position="365"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="615"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="domain" description="Gem-associated protein 5 TPR" evidence="5">
        <location>
            <begin position="962"/>
            <end position="1170"/>
        </location>
    </feature>
    <feature type="domain" description="Gem-associated protein 5 RBS" evidence="7">
        <location>
            <begin position="1289"/>
            <end position="1444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="201"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="740"/>
            <end position="793"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8028195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1700" mass="189244" checksum="9D14647A9F655C67" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BKJ8</accession>
    <name>A0A9Q1BKJ8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="34">Flavin-containing monooxygenase</fullName>
            <ecNumber evidence="34">1.-.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="36" type="ORF">HOLleu_30514</name>
    </gene>
    <organism evidence="36 37">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="36" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="36">Nanhai2018</strain>
            <tissue evidence="36">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Acts as a Baeyer-Villiger monooxygenase on a broad range of substrates. Catalyzes the insertion of an oxygen atom into a carbon-carbon bond adjacent to a carbonyl, which converts ketones to esters. Active on diverse carbonyl compounds, whereas soft nucleophiles are mostly non- or poorly reactive. In contrast with other forms of FMO it is non- or poorly active on 'classical' substrates such as drugs, pesticides, and dietary components containing soft nucleophilic heteroatoms. Able to oxidize drug molecules bearing a carbonyl group on an aliphatic chain, such as nabumetone and pentoxifylline. Also, in the absence of substrates, shows slow but yet significant NADPH oxidase activity. Acts as a positive modulator of cholesterol biosynthesis as well as glucose homeostasis, promoting metabolic aging via pleiotropic effects.</text>
    </comment>
    <comment type="function">
        <text evidence="19">Broad spectrum monooxygenase that catalyzes the oxygenation of a wide variety of nitrogen- and sulfur-containing compounds including xenobiotics. Catalyzes the S-oxygenation of hypotaurine to produce taurine, an organic osmolyte involved in cell volume regulation as well as a variety of cytoprotective and developmental processes. In vitro, catalyzes the N-oxygenation of trimethylamine (TMA) to produce trimethylamine N-oxide (TMAO) and could therefore participate to the detoxification of this compound that is generated by the action of gut microbiota from dietary precursors such as choline, choline containing compounds, betaine or L-carnitine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>(2E)-geranial + NADPH + O2 + H(+) = (1E)-2,6-dimethylhepta-1,5-dien-1-yl formate + NADP(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:54860"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16980"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:138375"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="29">
            <dbReference type="Rhea" id="RHEA:54861"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="31">
            <text>N,N-dimethylaniline + NADPH + O2 + H(+) = N,N-dimethylaniline N-oxide + NADP(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:24468"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16269"/>
            <dbReference type="ChEBI" id="CHEBI:17735"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.14.13.8"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="31">
            <dbReference type="Rhea" id="RHEA:24469"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>NADPH + O2 + H(+) = H2O2 + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:11260"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.6.3.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:11261"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>heptan-2-one + NADPH + O2 + H(+) = pentyl acetate + NADP(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:54836"/>
            <dbReference type="ChEBI" id="CHEBI:5672"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:87362"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:54837"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="30">
            <text>heptan-4-one + NADPH + O2 + H(+) = propyl butanoate + NADP(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:54852"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:89484"/>
            <dbReference type="ChEBI" id="CHEBI:89719"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="30">
            <dbReference type="Rhea" id="RHEA:54853"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>hexan-3-one + NADPH + O2 + H(+) = ethyl butanoate + NADP(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:54844"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:88764"/>
            <dbReference type="ChEBI" id="CHEBI:89891"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:54845"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>hexan-3-one + NADPH + O2 + H(+) = propyl propanoate + NADP(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:54848"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:89828"/>
            <dbReference type="ChEBI" id="CHEBI:89891"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:54849"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>hypotaurine + NADH + O2 + H(+) = taurine + NAD(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:74111"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57853"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:507393"/>
            <dbReference type="EC" id="1.14.13.8"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:74112"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>hypotaurine + NADPH + O2 + H(+) = taurine + NADP(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:69819"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57853"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:507393"/>
            <dbReference type="EC" id="1.14.13.8"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:69820"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>octan-3-one + NADPH + O2 + H(+) = ethyl hexanoate + NADP(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:54856"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:80946"/>
            <dbReference type="ChEBI" id="CHEBI:86055"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="28">
            <dbReference type="Rhea" id="RHEA:54857"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="32">
            <text>octan-3-one + NADPH + O2 + H(+) = pentyl propanoate + NADP(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:54840"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:80946"/>
            <dbReference type="ChEBI" id="CHEBI:87373"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="32">
            <dbReference type="Rhea" id="RHEA:54841"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>sulcatone + NADPH + O2 + H(+) = 4-methylpent-3-en-1-yl acetate + NADP(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:54864"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16310"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:138373"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:54865"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>trimethylamine + NADPH + O2 = trimethylamine N-oxide + NADP(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:31979"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15724"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58389"/>
            <dbReference type="EC" id="1.14.13.148"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:31980"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 33 34">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Microsome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 33 34">Belongs to the FMO family.</text>
    </comment>
    <comment type="caution">
        <text evidence="36">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.-.-.-" evidence="34"/>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BKJ8"/>
    <dbReference type="OrthoDB" id="66881at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004499">
        <property type="term" value="F:N,N-dimethylaniline monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016174">
        <property type="term" value="F:NAD(P)H oxidase H2O2-forming activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034899">
        <property type="term" value="F:trimethylamine monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000159">
        <property type="entry name" value="Dimethylaniline monooxygenase [N-oxide-forming]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000960">
        <property type="entry name" value="Flavin_mOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020946">
        <property type="entry name" value="Flavin_mOase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002257">
        <property type="entry name" value="Flavin_mOase_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050346">
        <property type="entry name" value="FMO-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23023">
        <property type="entry name" value="DIMETHYLANILINE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00743">
        <property type="entry name" value="FMO-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000332">
        <property type="entry name" value="FMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00370">
        <property type="entry name" value="FMOXYGENASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01125">
        <property type="entry name" value="FMOXYGENASE5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 33" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10 33 34" id="KW-0274">FAD</keyword>
    <keyword evidence="7 33 34" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="16" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="17 33 35" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="11" id="KW-0492">Microsome</keyword>
    <keyword evidence="15 33 34" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="12 33" id="KW-0521">NADP</keyword>
    <keyword evidence="14 33 34" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="37" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 35" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 35" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="35">
        <location>
            <begin position="507"/>
            <end position="528"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00045722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00045957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00047426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00047855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00047864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00047977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00048041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00048088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00048459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00048989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00048990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00049443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00049475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="PIRNR" id="PIRNR000332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="RuleBase" id="RU361177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="35">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="36">
        <source>
            <dbReference type="EMBL" id="KAJ8028313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="37">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="532" mass="60645" checksum="820972D5C1627153" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BKN5</accession>
    <name>A0A9Q1BKN5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_30435</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BKN5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8028249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="145" mass="16583" checksum="56E267AAA2764DD1" modified="2023-09-13" version="1">MVICSCWVWTNEGGGGEQGFGVSPSEEMFFSIIGNTLPRCFVGHLPFHILSCSCLSFVEVHILKMSVDKCVLTDLEVFPMKFKNPCTHLLVFMKDKYRKQAELSHIQPACCVFVQPCDKQFQVTLQMFLSAYDYKKNILSRFSFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BMN6</accession>
    <name>A0A9Q1BMN6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_28623</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BMN6"/>
    <dbReference type="OrthoDB" id="10063225at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="259"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="259"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8029270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="696" mass="78086" checksum="63845D1ED8E105F8" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BNG4</accession>
    <name>A0A9Q1BNG4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phosphodiesterase</fullName>
            <ecNumber evidence="9">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_29247</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="9">Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cyclic nucleotide phosphodiesterase family. PDE8 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="9"/>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BNG4"/>
    <dbReference type="OrthoDB" id="189220at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004114">
        <property type="term" value="F:3',5'-cyclic-nucleotide phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00156">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1300.10:FF:000002">
        <property type="entry name" value="Phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1300.10">
        <property type="entry name" value="3'5'-cyclic nucleotide phosphodiesterase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057304">
        <property type="entry name" value="PDE8-like_REC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023088">
        <property type="entry name" value="PDEase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002073">
        <property type="entry name" value="PDEase_catalytic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036971">
        <property type="entry name" value="PDEase_catalytic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023174">
        <property type="entry name" value="PDEase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11347">
        <property type="entry name" value="CYCLIC NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13426">
        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08629">
        <property type="entry name" value="PDE8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23198">
        <property type="entry name" value="PDE8A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00233">
        <property type="entry name" value="PDEase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00387">
        <property type="entry name" value="PDIESTERASE1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00126">
        <property type="entry name" value="PDEASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51845">
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        <location>
            <position position="593"/>
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        <ligand>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Holothuria leucospilota</name>
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            <taxon>Aspidochirotida</taxon>
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            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
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            <topology evidence="1">Multi-pass membrane protein</topology>
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    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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        <property type="match status" value="1"/>
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    </dbReference>
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        <source>
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    </evidence>
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        <source>
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    <sequence length="901" mass="100362" checksum="C2C0584916F6D3D4" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BR15</accession>
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        <submittedName>
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        </submittedName>
    </protein>
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        <name evidence="4" type="ORF">HOLleu_27599</name>
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        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
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            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
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    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Znf_C2H2_type"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13309">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
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            <end position="878"/>
        </location>
    </feature>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
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            <end position="1271"/>
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        </location>
    </feature>
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        <location>
            <begin position="339"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
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            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="386"/>
            <end position="400"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="2">
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        <location>
            <begin position="429"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
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            <end position="481"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
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            <begin position="551"/>
            <end position="575"/>
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    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="634"/>
            <end position="654"/>
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    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="675"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="695"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
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            <begin position="728"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="793"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="824"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="901"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="938"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="976"/>
            <end position="1028"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1029"/>
            <end position="1039"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1046"/>
            <end position="1057"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1058"/>
            <end position="1071"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1160"/>
            <end position="1231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1238"/>
            <end position="1247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1254"/>
            <end position="1271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8031009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1271" mass="142220" checksum="5834D7665177D4CB" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BRY4</accession>
    <name>A0A9Q1BRY4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_24777</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BRY4"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="402"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8031550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="455" mass="51927" checksum="3465895D732C95D1" modified="2023-09-13" version="1">MEDYQREALVDCFINSYVVFEPLDVTNNLLESAILDDDEVTKIENETLPWSRVQLLIAFVNRKAGCTFENLTDALKSCGKYCTLVKDLETSAAFLQKYNTKVEELVQCSSPGENFMYLDMLMSIAVSLDINDSLEICKHLSLSDECIAIVEKNKNPGLATMNICRKTRIVKVSSVQAFRNVFKSLSLAHQLASVDRYIKALEHPELELEVTKTEPDVLLETVKEIFRDRKTWFKVAKKIETLEPQIQDDVREDYVTTKIANVPHLPGALGPEIIVTITRSFPDTLLSLLRRAINDKTNFHIRGRPLRTFFYQYFEDILCTNIRDGSNGVQFVLKIAKVSALHRLYTAILNGKLAEDMLLTVQDTIGDNRVRRARMCVELDEDDYTNAQKYFEPSKTLIGHWGLSEEDKSPGTTNQGNPSDPLFVDPESFEMIQRLHQERQDYENALLRSGAFESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BS86</accession>
    <name>A0A9Q1BS86_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Acetylcholinesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_25288</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the type-B carboxylesterase/lipase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BS86"/>
    <dbReference type="OrthoDB" id="3200163at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003990">
        <property type="term" value="F:acetylcholinesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006581">
        <property type="term" value="P:acetylcholine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019695">
        <property type="term" value="P:choline metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050654">
        <property type="entry name" value="AChE-related_enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019819">
        <property type="entry name" value="Carboxylesterase_B_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43918">
        <property type="entry name" value="ACETYLCHOLINESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43918:SF4">
        <property type="entry name" value="CARBOXYLIC ESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00941">
        <property type="entry name" value="CARBOXYLESTERASE_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0719">Serine esterase</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040304829" evidence="4">
        <location>
            <begin position="19"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="Carboxylesterase type B" evidence="5">
        <location>
            <begin position="22"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8031922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="308" mass="33737" checksum="E666BF1AC43E676B" modified="2023-09-13" version="1">MKLLSVVVLAFSITIVESCCHPPSVDTGLGTVLGQRRNFSTRFLKESVEVHYYFGIPYARPPIDEMRFRQAEPAEPFEGVYNATEVPPSCIQERRPYFEHLTAGSTYSEDCLYLNIFVPETTPPNAAVMVFIHGGLFKWGSGSDFPHNPFPLVATGNVIVVTFNFRINIFGFLTSGDTAIPPNIALSDQQLALKWVNKNIAAFGGDPSKVTIFGEGSGAVSVGWHLLSPGSRGYVQRAVLQSGNPLYSSVELISPETAFKNIQTVADFADCTPPNPDNTTFIADCLRLKTVDELLDLQNQVSHVFLAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BSF7</accession>
    <name>A0A9Q1BSF7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">WASH complex subunit 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_25126</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CCDC53 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BSF7"/>
    <dbReference type="OrthoDB" id="268027at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071203">
        <property type="term" value="C:WASH complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030041">
        <property type="term" value="P:actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019309">
        <property type="entry name" value="WASHC3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13015">
        <property type="entry name" value="PROTEIN AD-016-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13015:SF0">
        <property type="entry name" value="WASH COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10152">
        <property type="entry name" value="CCDC53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="98"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="168"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="98"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8031809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="203" mass="22255" checksum="9494F3BDADB8294B" modified="2023-09-13" version="1">MLAFLNHFIIHTTRFLNKFSCVCEQKLLDLNQRIQKLEVTMNILEAKVLYTVSFVLFHHLRATVASIINPSVVASVLGYMGILLASIPGLENVTVEQTSASQGAESNLPQVTAGEGQEQKPSEEEAAPEAPPTMTVSQDPRYMKYFKMINMGVPIEPLRNKVLMDGLDPDLLLTPDAPAPPMTQEDSSESSFGSEESGNESSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BTX1</accession>
    <name>A0A9Q1BTX1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA-directed DNA polymerase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_26055</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BTX1"/>
    <dbReference type="OrthoDB" id="6077919at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33206:SF1">
        <property type="entry name" value="DNA-DIRECTED DNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33206">
        <property type="entry name" value="PROTEIN CBG10425"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8032509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="175" mass="20642" checksum="6D6DFB02DDCAA568" modified="2023-09-13" version="1">MLEFYYDFMIKFVHPEDFEYCEMDTDSAYLAISGDSLDDVIKPDMRNAFELEKHLWFPRTDTDEHKQHDKRTPGLFKLEWEGDGIVALNSKCYYCFGSEKEKVSCKGVNSRSKHLTKENYLKVLRNCSSVEAQNMGFRLWENQMQTYIQEKTALSYLYIKRKVNEDGVSTRPLDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BW98</accession>
    <name>A0A9Q1BW98_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_24289</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BW98"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8033907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="191" mass="21882" checksum="5D319EC2F5039246" modified="2023-09-13" version="1">MELLADRSVARMKYTERVLVRNRVLTLLLVYPALVENVIVRTDFIYKKGIVFGKNSVAVILMEIFYREVIPMLTLAVPRDVRATAIVLTAMIGTDVAQMQPAEYKTTYDNVTVTRDILVTELLADRSVVRMKYGERVLVRNRVLTLLLVYPALVENVIARTDFICKEEIVLDKKSVAVILVEIFYRTKLPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BXT5</accession>
    <name>A0A9Q1BXT5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">F-box/WD repeat-containing protein 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_21819</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BXT5"/>
    <dbReference type="OrthoDB" id="5580488at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="CDD" id="cd22148">
        <property type="entry name" value="F-box_DdgacFF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051075">
        <property type="entry name" value="SCF_subunit_WD-repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19872">
        <property type="entry name" value="UBIQUITIN LIGASE SPECIFICITY FACTOR/HREP PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19872:SF9">
        <property type="entry name" value="UBIQUITIN-BINDING SDF UBIQUITIN LIGASE COMPLEX SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="1 4" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="F-box" evidence="6">
        <location>
            <begin position="434"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="521"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="562"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="603"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="643"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="776"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="49"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="270"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="53"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="103"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="118"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8034817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="810" mass="92179" checksum="FBD73E2AB1E76337" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BYF0</accession>
    <name>A0A9Q1BYF0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_19461</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8035703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BYF0"/>
    <dbReference type="OrthoDB" id="6431331at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010463">
        <property type="entry name" value="DUF1057"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47533:SF4">
        <property type="entry name" value="AB HYDROLASE-1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47533">
        <property type="entry name" value="PROTEIN CBG21859"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06342">
        <property type="entry name" value="DUF1057"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8035703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="373" mass="41852" checksum="4CF638312EA642F4" modified="2023-09-13" version="1">MTRCCRREFLNSCRNFIRTRYNRDQNLYHVLHKTGRSIQESLLSSNNIRGISAYQSPPIQLTTDAGSFFTMKEVTVKALGDQFNVIYAHAGPEDGDAIITIHGSPASHKGFQNMAEPLVAAGYQVIVPNLPGMGWTQLPPSGCYNFSSFHKAQVIQGVAEALKMEKIKMLCGHSMGCQAIFQMPRLPSFFAQCESICLISSLGLRPHRIASPWFFKRRLFATYYKMCALPVLGPLLVEFASPIMVILGLKGRTKLAQVNSIQEAAMFNWEEFTAGVDELRRQKIPVLIFSSDKDGLISFDIAQELVARFGITQDNTAWYQGVKKDRDDFKESTLQRAVFIKGGDHSIFTKRVDVIAPEQLQFLNSLDQPAARQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BYS3</accession>
    <name>A0A9Q1BYS3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_22574</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BYS3"/>
    <dbReference type="OrthoDB" id="6142091at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8035372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="145" mass="17454" checksum="4D919A2BFDDE34D1" modified="2023-09-13" version="1">MPFNLDEFVASPSVEELDSLKKSEIVKVEKHYGIEFQPLMRKDEIKRYVLEYLVDESILPSTVLKTVITVPTDNTFELKRLEIEMNKEVRLKEMEREVQKEKEEREMQMQKEREEREERERRNRLGYWGIRCFKARKICPQISGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C065</accession>
    <name>A0A9Q1C065_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_19406</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8035661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C065"/>
    <dbReference type="OrthoDB" id="6128308at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21301">
        <property type="entry name" value="REVERSE TRANSCRIPTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21301:SF10">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8035661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="77" mass="9126" checksum="6C0F9176EB21BB7E" modified="2023-09-13" version="1">MGTRMAPSYANLFMAEFERNLLHSSYHKPSFYVRFIDDIFFIWEKGPDELQNFFQLPNGLHPTIKFTMESSCNNIPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C0E2</accession>
    <name>A0A9Q1C0E2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Agrin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_19933</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C0E2"/>
    <dbReference type="OrthoDB" id="192611at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00104">
        <property type="entry name" value="KAZAL_FS"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.30">
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036058">
        <property type="entry name" value="Kazal_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050653">
        <property type="entry name" value="Prot_Inhib_GrowthFact_Antg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10913:SF45">
        <property type="entry name" value="FOLLISTATIN, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10913">
        <property type="entry name" value="FOLLISTATIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00050">
        <property type="entry name" value="Kazal_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07648">
        <property type="entry name" value="Kazal_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00280">
        <property type="entry name" value="KAZAL"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100895">
        <property type="entry name" value="Kazal-type serine protease inhibitors"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51465">
        <property type="entry name" value="KAZAL_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0722">Serine protease inhibitor</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040404086" evidence="4">
        <location>
            <begin position="18"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="Kazal-like" evidence="5">
        <location>
            <begin position="19"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="Kazal-like" evidence="5">
        <location>
            <begin position="69"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="Kazal-like" evidence="5">
        <location>
            <begin position="120"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="Kazal-like" evidence="5">
        <location>
            <begin position="171"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="Kazal-like" evidence="5">
        <location>
            <begin position="225"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8036067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="279" mass="31483" checksum="6513FE1A53F6AB00" modified="2023-09-13" version="1">MKILILFVATAIITCDAFVWNRKKCSTSCITVYDPVCGSDGETYSNSCELEKQKCWSGDSTLKEVPCGVQGKKRCSEKCTTDYNPVCGSDKVTYINQCEFWKAQCQDPTLILIGHGECDPIGKKRCSEKCTTDYNPVCGSDKVTYINQCEFWKAQCHDSTLILKGRGECDPIGKKRCSEKCTTDYNPVCGSDKVTYINQCEFWKAQCQDPTLILKGRGECDPIGKRPVERCSEACTREYIPVCGSDGVTYSNQCEFYKAQYQYPTLKLWRRGECDRIGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C2E4</accession>
    <name>A0A9Q1C2E4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">26S proteasome non-ATPase regulatory subunit 12</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_17590</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the proteasome subunit p55 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8036924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C2E4"/>
    <dbReference type="OrthoDB" id="268763at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000070">
        <property type="entry name" value="26S proteasome non-ATPase regulatory subunit 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054559">
        <property type="entry name" value="PSMD12-CSN4-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040134">
        <property type="entry name" value="PSMD12/CSN4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040896">
        <property type="entry name" value="RPN5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10855:SF1">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10855">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 12/COP9 SIGNALOSOME COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22241">
        <property type="entry name" value="PSMD12-CSN4_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18098">
        <property type="entry name" value="RPN5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0647">Proteasome</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="3">
        <location>
            <begin position="186"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8036924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="404" mass="47179" checksum="874CF90737639D2A" modified="2023-09-13" version="1">MAADMQSTSRVLVAIVRMCFEVKDWDALNENILLLSKRRSQLKQAVQKMVQEACTYLDKTPDLETKLKLIDTLRTVTAGKIYVEIERARLTMTLAKIKEDQGDIAEAATILQELQVETFGSMERKEKVEFILEQMRLCLAKKDYIRTQIISKKISLKFFESTAEEVQALKLRFYKLMIEIDEHEGAYLDICKHYRAIFDTPIIKDKTEQWKEALKNTVLYLILAPFDNEQSDLIHRVSLEKQLEDIPIYRDLLKNFTTSELMRWQQVCHIYEKELKVGSEGNPATGVFADTEQGQKRWSDLKNRVVEHNIRVMAKYYTRLHMKRMAELLELSEMEAEEFLSNLVVKKTVYAKVDRLAGIVDFTAHKDPNEVLNSWSNNLNELMQLVNKTTHLINKEEMLHQFTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C355</accession>
    <name>A0A9Q1C355_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="16">TWiK family of potassium channels protein 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HOLleu_18355</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Nanhai2018</strain>
            <tissue evidence="16">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the two pore domain potassium channel (TC 1.A.1.8) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C355"/>
    <dbReference type="OrthoDB" id="297496at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015271">
        <property type="term" value="F:outward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022841">
        <property type="term" value="F:potassium ion leak channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030322">
        <property type="term" value="P:stabilization of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003280">
        <property type="entry name" value="2pore_dom_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003092">
        <property type="entry name" value="2pore_dom_K_chnl_TASK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11003:SF334">
        <property type="entry name" value="FI03418P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11003">
        <property type="entry name" value="POTASSIUM CHANNEL, SUBFAMILY K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01333">
        <property type="entry name" value="2POREKCHANEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01095">
        <property type="entry name" value="TASKCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12 16" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0630">Potassium</keyword>
    <keyword evidence="6" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="108"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="139"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="246"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="278"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="307"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="15">
        <location>
            <begin position="107"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="15">
        <location>
            <begin position="254"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="187"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="195"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF07885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ8037522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="347" mass="39128" checksum="6124CF821B9129E7" modified="2023-09-13" version="1">MTQQRSALWRASVPHIALIIFYMFYLLAGGVLFHWLESKPTEKFVQSFEKQEGKIEADIDVLFRNYSKLNKTKSEEAKLDVLIDKLINKSEHFDRGAWDPDLYESPNAWSVSSAMLFCMITITTIGYGDLVPNTVAGRIAVILYSCIGIPLSFLLIADTGGLLARLASSSTAYVRRRLCGKNDQIFQPLEEDKDETNPKPDEYARQSGGGNPLSESDTARRNPQISLEAEPSYLSNVPEEDKEGTVPILVVVFILTTYICIGALSFSLSEGWSFTEGLYFTFITLTTIGYGDLYPSRHVQNNTMIPILVYILFGLCLVSMSVSLVMWRFMKLVTWMRNKVEYLCCCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C5M1</accession>
    <name>A0A9Q1C5M1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Low-density lipoprotein receptor-related protein 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_15741</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C5M1"/>
    <dbReference type="OrthoDB" id="9971251at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003410">
        <property type="entry name" value="HYR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000033">
        <property type="entry name" value="LDLR_classB_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24273">
        <property type="entry name" value="FI04643P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24273:SF32">
        <property type="entry name" value="HYALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02494">
        <property type="entry name" value="HYR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00058">
        <property type="entry name" value="Ldl_recept_b"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00135">
        <property type="entry name" value="LY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63825">
        <property type="entry name" value="YWTD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50825">
        <property type="entry name" value="HYR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51120">
        <property type="entry name" value="LDLRB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CUB" evidence="5">
        <location>
            <begin position="63"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="4">
        <location>
            <begin position="158"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="4">
        <location>
            <begin position="201"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="HYR" evidence="6">
        <location>
            <begin position="273"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="HYR" evidence="6">
        <location>
            <begin position="354"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8038346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="444" mass="49347" checksum="0AFFA626D503195A" modified="2023-09-13" version="1">MKHISSNIVNLTRSVKFYSSFLYYFRFNVKTSQSLLAAVDEQAKEENHEVIFNFDVRLCPDVCNNEDLIATSDLQLIFSPNFPRNYPNNVHCTSHITSPEDTVIELTFYHFQLEDSDDCNKDSLSVRIENNSNHRHINVTIGIFHSEPFGLRIDVNSRLLYWTDAELGTISVSNLDGEYRNTIIHTELDKPGAIVTEPYKGYIYFTDWGTHPKIERADGDGRNRLVLVNSDIVSPRSLAVDLKDVVVPDTCPRADCDWLCLPTPNGQHTCLSDDTTPPVFLNCPENIIIWVAPGVSSSQVSWDDVSLEDDVDVIISSSHLSGSTFRLGTETVKIAARDFAGNMAWCNFSATVIEDNSPPVIISCPSDISVSLSSSAETRQIVTWDEPVLSDNAGDAYWLVGPSSSGTAFPIGSTHITYVASDRFGNQAQCSFTVTVSGNVQLYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C5U3</accession>
    <name>A0A9Q1C5U3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_15882</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C5U3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8038451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="78" mass="8978" checksum="C18745353F8BD587" modified="2023-09-13" version="1">MESSREFNFNAAFLELSRSENCLRYVPITTTVCSRRSSLVIIRSWNQFLRYSTLQVYHCAIVNVHALPALGPDQNTIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-04-02" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C683</accession>
    <name>A0A9Q1C683_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Histone-lysine N-methyltransferase EHMT1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_16512</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="5792673at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046974">
        <property type="term" value="F:histone H3K9 methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002039">
        <property type="term" value="F:p53 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20905">
        <property type="entry name" value="EHMT_ZBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10543">
        <property type="entry name" value="SET_EHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.270.10:FF:000005">
        <property type="entry name" value="Euchromatic histone-lysine N-methyltransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043550">
        <property type="entry name" value="EHMT1/EHMT2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047762">
        <property type="entry name" value="EHMT_CRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007728">
        <property type="entry name" value="Pre-SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46307">
        <property type="entry name" value="G9A, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46307:SF4">
        <property type="entry name" value="G9A, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21533">
        <property type="entry name" value="EHMT1-2_CRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05033">
        <property type="entry name" value="Pre-SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00468">
        <property type="entry name" value="PreSET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
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        <location>
            <begin position="1165"/>
            <end position="1197"/>
        </location>
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    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="1198"/>
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            <end position="1297"/>
        </location>
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        <location>
            <begin position="1335"/>
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        </location>
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        <location>
            <begin position="1457"/>
            <end position="1520"/>
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            <begin position="1523"/>
            <end position="1640"/>
        </location>
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            <begin position="1"/>
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    <feature type="region of interest" description="Disordered" evidence="6">
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        <source>
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        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
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    <sequence length="1666" mass="182656" checksum="928A784D5DFE4165" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
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        <lineage>
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            <taxon>Eleutherozoa</taxon>
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        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain evidence="2">Nanhai2018</strain>
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        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="2"/>
            <end position="51"/>
        </location>
    </feature>
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            <dbReference type="Pfam" id="PF01826"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAJ8038796.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="83" mass="9197" checksum="A48025D4A374E7E4" modified="2023-09-13" version="1">MVWGNCTCMATCKDPKAKISCYYSSCSGDEGCYCPSGFLMEREHCIPRNECGCFLPEADEVLPLFRSFAFLTEGSVSVRSDSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C6L2</accession>
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        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
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        <dbReference type="NCBI Taxonomy" id="206669"/>
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                <person name="Chen T."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain evidence="2">Nanhai2018</strain>
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        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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        <location>
            <begin position="4"/>
            <end position="91"/>
        </location>
    </feature>
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        <source>
            <dbReference type="Pfam" id="PF07686"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAJ8039355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="244" mass="27174" checksum="78E2F7469B885F97" modified="2023-09-13" version="1">MDCPAKVYVELGKDGIINCQILNNGDVYWYKGNSTKTSPIVRLENGQKKISQESGRYDIDEEGSLIIKKIEEQNYGFYSVVNFHANDKHETDQIKVEIAVKPQTCPVIAGCNDCNQCVLSEPKEGNISCSITGIRPLVHLSIVIDSRSNVQVTRSVSKETYDPMTDTWNTTATIEYSESYDCTSLLQLRCVTENEDPLQFSDSVANITSGNFLKFCTVTSGVRFLLGALRGWKFLWGGGVPFTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C7N7</accession>
    <name>A0A9Q1C7N7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Cell cycle checkpoint protein RAD17</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_15221</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the rad17/RAD24 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="10265971at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003689">
        <property type="term" value="F:DNA clamp loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000077">
        <property type="term" value="P:DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033314">
        <property type="term" value="P:mitotic DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18140">
        <property type="entry name" value="HLD_clamp_RFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001661">
        <property type="entry name" value="RAD17 checkpoint clamp loader component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004582">
        <property type="entry name" value="Checkpoint_prot_Rad17_Rad24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057927">
        <property type="entry name" value="RAD24-like_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047854">
        <property type="entry name" value="RFC_lid"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12172">
        <property type="entry name" value="CELL CYCLE CHECKPOINT PROTEIN RAD17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12172:SF0">
        <property type="entry name" value="CELL CYCLE CHECKPOINT PROTEIN RAD17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03215">
        <property type="entry name" value="Rad17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25812">
        <property type="entry name" value="RAD24_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Checkpoint protein RAD24-like helical bundle" evidence="9">
        <location>
            <begin position="373"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="336"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="588"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="36"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF25812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8040818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="635" mass="71819" checksum="6CD100AF4729E43C" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C8A6</accession>
    <name>A0A9Q1C8A6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Membralin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_13876</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8039771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C8A6"/>
    <dbReference type="OrthoDB" id="6779347at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904294">
        <property type="term" value="P:positive regulation of ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034976">
        <property type="term" value="P:response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019144">
        <property type="entry name" value="Membralin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21650:SF4">
        <property type="entry name" value="MEMBRALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21650">
        <property type="entry name" value="MEMBRALIN/KINETOCHORE PROTEIN NUF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09746">
        <property type="entry name" value="Membralin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="328"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="369"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="402"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="450"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="510"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="592"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="657"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="24"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="510"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8039771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="698" mass="78806" checksum="8D57F6496FABFACA" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C8J1</accession>
    <name>A0A9Q1C8J1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">F-box/LRR-repeat protein 12</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_14399</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C8J1"/>
    <dbReference type="OrthoDB" id="10257471at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="39"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8040178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="368" mass="42737" checksum="C4A7DB599AED8357" modified="2023-09-13" version="1">MADNKSCAFVQKDQKQVKKLPVTGDDGSNDDKSDRVLEVVAIEDLPECLLLRIFSYLKGVEDRCSVARTCQDWNRVIKDGAVWKGFTLQDTSSDYQTVKHLIACGLASSLLDLDLLMSANNENCVRFLHDRKVWPLFTKLENLRITNIDICAFDFELLPSTLKSLVIYPSKHFLRPIPARVKCSRQVFPNLEYFEIEDCEDVFANDQIEQLCGLEYLLSSAKLHTVILKLMHFRFSHQSLSNMSEKMKTVKYVCLHAHSIKQRGFQTFLSNLTNVETLNLQFADIDADMETVILASIICLKELKEIYLSMFWELRSEHIKKMVENLPNLMFIQVYRCRRVKKEHLAEIEKMRDGLKCQFPDFMRLSFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C8S4</accession>
    <name>A0A9Q1C8S4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Sulfotransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_12173</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C8S4"/>
    <dbReference type="OrthoDB" id="10010208at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053259">
        <property type="entry name" value="Golvesin-related_Golgi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32301">
        <property type="entry name" value="COUNTIN RECEPTOR CNR3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32301:SF6">
        <property type="entry name" value="GOLVESIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8041373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="378" mass="43023" checksum="0B7A625FC8DD10E5" modified="2023-09-13" version="1">MEQSTLDGIMKYITGGKGKSIYILVTVFVVVLMLKLKTDAENWRMESPLMPPKIWLEAKRVADEVDPPDLHNEYALPTKQVLPIDGPVYNNALPNPYVTGRNITDYSKSIMAFMHNPKSGGTTLKDCILTLTQELHQRRPILLAGNDSRVEIQENILNGIINPEKYNFIMGGASLGICESFSSRKCSYFTILREPYDRMISHYFFCKDGGKSGAPCANKSIEEFTLRVGSLFFSQLAVTVTCHCDNNCDNISKQPWQCVSDHSSYYKNIEYKDEFLQFLIQHLDSYFAVIGLTEEYDTTLTILQSTFGVPFHNLCSGFQRNAGKYGSKGKASSEERKLEAKKRMLASEAVRRTLYPDVMLYERAKQIFQMQKEKLHLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C8T0</accession>
    <name>A0A9Q1C8T0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">DNA excision repair protein ERCC-6-like 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_15213</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="448448at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000019">
        <property type="entry name" value="DNA excision repair protein ERCC-6-like 2 isoform X1"/>
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        <property type="entry name" value="DNA EXCISION REPAIR PROTEIN ERCC-6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45629">
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        <property type="entry name" value="Helicase_C"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HELICc"/>
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        <location>
            <begin position="6"/>
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            <begin position="254"/>
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            <begin position="629"/>
            <end position="780"/>
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            <begin position="128"/>
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            <begin position="834"/>
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            <begin position="1454"/>
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            <begin position="181"/>
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            <begin position="869"/>
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            <begin position="908"/>
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            <begin position="1153"/>
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            <begin position="1248"/>
            <end position="1273"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50304"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8040811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1477" mass="166780" checksum="E17CC7EE999813AF" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C8Y6</accession>
    <name>A0A9Q1C8Y6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Histamine N-methyltransferase</fullName>
        </submittedName>
    </protein>
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        <name evidence="1" type="ORF">HOLleu_14466</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
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            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
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    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8040234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="293" mass="33194" checksum="1FB14999B1CA8D4B" modified="2023-09-13" version="1">MSLRYLQNFPSHYWKSYRGLRDNFYECPLEYDIGNEAFQTMVVDVLPKQESLNALGIGSGQGELDCINMGRMLTRVSRIDNTVVEPSLEAIEVYKSLFSGDLSDRVTTSWYQETFQEYQKRRESSTGPTEKFHFISAVHSLYYTGSDGSSIGYLIDLLEKDGVLFIAIQNDESGFIKFFGKVAESYGPLYNNTTQGYTTSQVVKLLDADGRVTYNVVKNRHNIDVTACFEADSSKGSLVLDFLTHVVDFKSTISQNVLESLLDYLKSPDCSEILSDGRIIFHEDWDAIIITKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C990</accession>
    <name>A0A9Q1C990_HOLLE</name>
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        <recommendedName>
            <fullName evidence="2">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
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        <name evidence="3" type="ORF">HOLleu_15078</name>
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    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
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        <lineage>
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            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
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            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
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        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="evidence" value="ECO:0007669"/>
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        <property type="evidence" value="ECO:0007669"/>
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        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon gypsy-like Protein"/>
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        <property type="entry name" value="Uncharacterized protein"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
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        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
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        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
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        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN CBG26694"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="229"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="514"/>
            <end position="701"/>
        </location>
    </feature>
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        <location>
            <begin position="550"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="599"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="659"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="673"/>
            <end position="683"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8040705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="734" mass="82231" checksum="1ED9746E94397E22" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CB96</accession>
    <name>A0A9Q1CB96_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_12503</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CB96"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="167"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="288"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="173"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="188"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="288"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="298"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8041631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="368" mass="41837" checksum="72864621667E832B" modified="2023-09-13" version="1">MEDTAVAQVLVEHQILSSDRGAEHKTNRDVTNRMSSGVVSNDNPHTHNSRGKFVNVTASLRVIRELIDALLDDKFDFERGEIARKGPAENKATEHRNTYHFYKHNPSQSSARHDKLSDSEKIHGKEVQQQQMAYKDIPHKSFDGNKRVNVISPSLREGRHGRFPISRTRRSFSAKSQTSPSELQVPAKYKRTWHHSEKHHKPDDLQSYVCRTHISTNNNNRNGDLCRKESIRNQEFVIGDDNDLPESMKVCDAESPSVKRCKLLEESIGLGSSSSSLIGQMKPSLKFTISPSTMTSDASERHPSRKEENLARRVPTKIRDVGMDEGHMRKDTCLPILAYLKKNVKNFMDRVAIPSHILQEEAVEEVLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CBZ6</accession>
    <name>A0A9Q1CBZ6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Biogenesis of lysosome-related organelles complex 1 subunit 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_13101</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8042112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CBZ6"/>
    <dbReference type="OrthoDB" id="2372305at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031083">
        <property type="term" value="C:BLOC-1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024857">
        <property type="entry name" value="Cappuccino"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16230:SF3">
        <property type="entry name" value="BIOGENESIS OF LYSOSOMAL ORGANELLES COMPLEX-1, SUBUNIT 4, CAPPUCCINO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16230">
        <property type="entry name" value="CAPPUCCINO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8042112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="171" mass="19164" checksum="93E0E41BCC4CC61A" modified="2023-09-13" version="1">MADATAKKNLDNIFTSFIDNLGHVQEKLRDDDHIQAEETEHGDSLETIVAATAADYAAFLSADPEKERTQLDEKIEELLTRLDEFCAVVDIKFVAIVKKDVAIVEEQVNTAEKDLGALTSVKNLIMSFPLFSQKRTKNPAHKPLRKYVPPEIFRTEDYISPAEQLDQSGEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CCR2</accession>
    <name>A0A9Q1CCR2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_10211</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CCR2"/>
    <dbReference type="OrthoDB" id="6508513at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF1">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8043218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="148" mass="17267" checksum="3E747807380665A1" modified="2023-09-13" version="1">MGKELGLTGKDLVTFAQDRDKTARDERQKEREHQRELKELEVQKAAEEAKAKPIPQHDVHSILKKSHFPSFDDKVDEIDCYLKRFERFVETMGYPRDKMAVLLSTVLKGKSLNYDRLKSALLKSYNLNADGFRRKFRNSKLDSGETYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CG52</accession>
    <name>A0A9Q1CG52_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_07438</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CG52"/>
    <dbReference type="OrthoDB" id="6272653at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051077">
        <property type="entry name" value="Ca-dependent_lectin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24024">
        <property type="entry name" value="PULMONARY SURFACTANT-ASSOCIATED PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24024:SF18">
        <property type="entry name" value="SHORT-CHAIN COLLAGEN C4-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8044647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="280" mass="30596" checksum="44264BFA7E395A53" modified="2023-09-13" version="1">MTKERKRKRLYGTMPRKSLKLERNEVFRDLLVHPVLQDIQDIPVHLAHKGPQGLKVLLEARAPLVHPVGTVGMQLLSLMLVTFVPNRKCRLVVQSLFAGGARTVQKLLTYCIVGIAGGSRHRDLPGGGANLLCLPSSPSFVNPVDGVGDARAFLYSVQYQISNYPAWQTYHLQDVSCAVCKATGRYSHLMIPAMQSCPSSEWTLEYRGYLMAERSHTVHHKSMFICMDQEPQPVPNSGGAATASSALLNLVEGKCSTSGGGLPCGQYPDGKEFTCAVCTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CG82</accession>
    <name>A0A9Q1CG82_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Beta-1 adrenergic receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_11536</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8044153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CG82"/>
    <dbReference type="OrthoDB" id="9996086at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071880">
        <property type="term" value="P:adenylate cyclase-activating adrenergic receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043410">
        <property type="term" value="P:positive regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00637">
        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248">
        <property type="entry name" value="ADRENERGIC RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248:SF120">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="22"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="56"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="99"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="137"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="186"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="293"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="323"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="34"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="225"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="227"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8044153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="365" mass="42432" checksum="6D5BF56DFA2BB9E5" modified="2023-09-13" version="1">MDLANGNDTDYILVDVPVSLQVIYFILGIAILVTNLIVIIAFLLDRDIRSVPTNIFIFNLSISDFLMGTDLIFHFSWSNESGLIVAPLYGTLWCQLNTAIYYQALLVSVWTVVVISWDRLQMVSNPFKYRQRTVRRAVWIVITLWSILLLYVCFNLFVLPMFYDDPIDQVYCIETDLPNDYVLESWWTDFLLPFVILVVINVRVVTQLRKATIEKYDTQMKEMYGTDASSNQNDRTSSKNYDITSNSDGNENTSEFKMIGEETTLSTTLKLPELRIQKAVRLELGKIQRTTKILEIFVGVFVICLLPYYIVILIDCFTSLPDWVILASYLNVCANSLVNPFMYAFMCRKFRHRFELMRKCRRRAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CGU7</accession>
    <name>A0A9Q1CGU7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">LYR motif-containing protein 9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_11642</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I LYR family. LYRM9 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8044239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CGU7"/>
    <dbReference type="OrthoDB" id="190541at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="CDD" id="cd20269">
        <property type="entry name" value="Complex1_LYR_LYRM9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008011">
        <property type="entry name" value="Complex1_LYR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045291">
        <property type="entry name" value="Complex1_LYR_LYRM9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052151">
        <property type="entry name" value="Complex_I_LYR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47061">
        <property type="entry name" value="LYR MOTIF-CONTAINING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47061:SF1">
        <property type="entry name" value="LYR MOTIF-CONTAINING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05347">
        <property type="entry name" value="Complex1_LYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Complex 1 LYR protein" evidence="3">
        <location>
            <begin position="11"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00025757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00026234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8044239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="78" mass="9260" checksum="60982C225B4A10FF" modified="2023-09-13" version="1">MSLSKESVRTAQQLYKYLWRVCEKLPKDSQAHYKHFVRQSFNSHADETDPERVEEIIQQALKDADWVLKKISYFIVSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CHI2</accession>
    <name>A0A9Q1CHI2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Proto-oncogene c-Fos</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_08438</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CHI2"/>
    <dbReference type="OrthoDB" id="10637686at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000837">
        <property type="entry name" value="AP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23351:SF24">
        <property type="entry name" value="ACTIVATING TRANSCRIPTION FACTOR 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23351">
        <property type="entry name" value="FOS TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07716">
        <property type="entry name" value="bZIP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00042">
        <property type="entry name" value="LEUZIPPRFOS"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="6">
        <location>
            <begin position="279"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="126"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="274"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="126"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8045427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="456" mass="50547" checksum="1120351C373BF6DD" modified="2023-09-13" version="1">MTKGGPNQSHWKLLEGMCVCESSNSETCSSSAPSPIHDSGADSVDVQAADSAFRQILEPFPLNEENSIVSPNLQHETLPDVADNNSEEQTLSVLYSVTISASESDDANFLLTPVSKTLSSVEECNDTSVLDESSQEHLSNSSDIKQDEEHFNISLQVSTEPVFSNDEQSQEPTQMGGEPHGPSMMLLPGEGVTVKSMLPLSPKETEAFDEKKSLEALDSLVMESGHEVLSQDLQNVDTTPFVKEELKERIKLRRQKKGLPNLEVGEESPKPDILTAEEEEKRRIRRERNRIAAAKCRRKKKEKASEKRKITEMIREQNLALLDELSQLMDEKARLQAMWTNHMKECKRRDMLELQLPELTSVMIRTTPELDLSDADSMGEEPLLINEDSSDSSSVDLTSDVGDQMIDSSFLDAMTEAPGPSTTTCNIQEAVTLLNSVDGDCITSTGNLQFEFLSSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CHS1</accession>
    <name>A0A9Q1CHS1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA-directed RNA polymerase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_07568</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8 11">Belongs to the archaeal rpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CHS1"/>
    <dbReference type="OrthoDB" id="282270at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001193">
        <property type="term" value="P:maintenance of transcriptional fidelity during transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006283">
        <property type="term" value="P:transcription-coupled nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10508">
        <property type="entry name" value="Zn-ribbon_RPB9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.10:FF:000004">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012164">
        <property type="entry name" value="Rpa12/Rpb9/Rpc10/TFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001529">
        <property type="entry name" value="Zn_ribbon_RPB9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034012">
        <property type="entry name" value="Zn_ribbon_RPB9_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001222">
        <property type="entry name" value="Znf_TFIIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239:SF1">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02150">
        <property type="entry name" value="Zn_ribbon_RPB9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01096">
        <property type="entry name" value="Zn_ribbon_TFIIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005586">
        <property type="entry name" value="RNApol_RpoM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00661">
        <property type="entry name" value="RPOL9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00440">
        <property type="entry name" value="ZnF_C2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51133">
        <property type="entry name" value="ZF_TFIIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8 11" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="3 9 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 9" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIS-type" evidence="12">
        <location>
            <begin position="60"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR005586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR005586-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8044734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="103" mass="12027" checksum="E78C162388FDE956" modified="2023-09-13" version="1">MLYPKEDKENKLLLYACRNCDFQQEADNMCVYVNKVTHEVDELTQIVGDVIADPTLPRTEDHPCPQCRGKEAVFFQSQTNRAEEGMRLYYVCCNPTCQHRYTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CIN5</accession>
    <name>A0A9Q1CIN5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Proto-oncogene c-Rel</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_08669</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CIN5"/>
    <dbReference type="OrthoDB" id="7881762at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.340">
        <property type="entry name" value="Rel homology domain (RHD), DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000451">
        <property type="entry name" value="NFkB/Dor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030492">
        <property type="entry name" value="RHD_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011539">
        <property type="entry name" value="RHD_DNA_bind_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037059">
        <property type="entry name" value="RHD_DNA_bind_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24169:SF28">
        <property type="entry name" value="NUCLEAR FACTOR NF-KAPPA-B P110 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24169">
        <property type="entry name" value="NUCLEAR FACTOR NF-KAPPA-B PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00554">
        <property type="entry name" value="RHD_DNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01204">
        <property type="entry name" value="REL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50254">
        <property type="entry name" value="REL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RHD" evidence="1">
        <location>
            <begin position="8"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8045625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="111" mass="12396" checksum="BE7D2E75956D5A14" modified="2023-09-13" version="1">MAGDNRAPKPKDHRFRYPCEGRTAGALLGVRSTQKKKTCPRSEAYMKIYHISFQVQNLTTRAMIVGSLVTNKGDKPHPFRLVGNSCEDGIVKKIVDAKSPKAKFESWAFSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CJ80</accession>
    <name>A0A9Q1CJ80_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Seipin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_08750</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CJ80"/>
    <dbReference type="OrthoDB" id="3990054at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140042">
        <property type="term" value="P:lipid droplet formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23995">
        <property type="entry name" value="Seipin_BSCL2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009617">
        <property type="entry name" value="Seipin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21212">
        <property type="entry name" value="BERNARDINELLI-SEIP CONGENITAL LIPODYSTROPHY 2 HOMOLOG BSCL2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21212:SF0">
        <property type="entry name" value="SEIPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06775">
        <property type="entry name" value="Seipin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="17"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="219"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="342"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="347"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="365"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8045695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="403" mass="46355" checksum="97DA8074344E3C1F" modified="2023-09-13" version="1">MKMGNGKRGLVDKTKSIIVSAVLLVSIVSVQVWLSVFGYFTFYYVYMPVERYEQPVFLEYSSCKQFTGDERCRFPGSNITLRTDYSQNRVLMRGQKYDIIIDLELPQSPVNQKQGVFMVELSLYSENGEVTATTSKPVMLKYRSSLLRIFDTLVFAPFFLLGYRDESQYLSVPLMTNFVDHPNKPVVGAYIEVQARQVEIYSANFLIRAQFSGLRYLMYYWPIITAVFIICFNFVIMTIISYLSWNQISSFFFKEDAGVMEDDPLVEMEDENDIVSFKISEREDKEQAVRTKVNREDLLKELKSSTGATKPWSSIFSSKKQFDSDDSDDDILLCSDFTERRTKDSPTRVSDVTAKSGSSDSFETIDDRREEDVTMGTSEHDKSEDLGMQGARFRSKKASLEFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CJX5</accession>
    <name>A0A9Q1CJX5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ankyrin repeat, SAM and basic leucine zipper domain-containing protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Germ cell-specific ankyrin, SAM and basic leucine zipper domain-containing protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HOLleu_05488</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Nanhai2018</strain>
            <tissue evidence="17">Muscle</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Interacts with DDX4, PIWIL1, RANBP9 and TDRD1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CJX5"/>
    <dbReference type="OrthoDB" id="448455at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071546">
        <property type="term" value="C:pi-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09521">
        <property type="entry name" value="SAM_ASZ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042650">
        <property type="entry name" value="Asz1_SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24157">
        <property type="entry name" value="ANKYRIN REPEAT, SAM AND BASIC LEUCINE ZIPPER DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24157:SF3">
        <property type="entry name" value="ANKYRIN REPEAT, SAM AND BASIC LEUCINE ZIPPER DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10 14" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-0221">Differentiation</keyword>
    <keyword evidence="12" id="KW-0469">Meiosis</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0943">RNA-mediated gene silencing</keyword>
    <keyword evidence="9" id="KW-0744">Spermatogenesis</keyword>
    <feature type="repeat" description="ANK" evidence="14">
        <location>
            <begin position="116"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="14">
        <location>
            <begin position="188"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="14">
        <location>
            <begin position="221"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="16">
        <location>
            <begin position="309"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="277"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="32"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="48"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00030354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ8046717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="519" mass="57351" checksum="7FA439C216BEA5DB" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CMY9</accession>
    <name>A0A9Q1CMY9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_01044</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CMY9"/>
    <dbReference type="OrthoDB" id="10060990at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46880">
        <property type="entry name" value="RAS-ASSOCIATING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46880:SF9">
        <property type="entry name" value="ZINC FINGER PROTEIN 862"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="257"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="271"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8048647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="299" mass="33867" checksum="B1CF9E854A26B516" modified="2023-09-13" version="1">MTGKKSGVGARLKEHQPKLQTIHCIAHRLELAIKDAAKSTAMFGKMGRFLANIYFFYHNSTHNRGLLRRTAEALGIKRFIPTRTGGTRWVVHTLNALTNLFAGYPAIVQHLLEIKDQANTSTDSKGKSAGFLKLLLSRQFLEFLNFMVDVTSVLGQLSQSFQKTEINMSEIPKLVKDTVTSLDKLKSHFKPVHSASWEKLAIKYKTSAPNLFCMVDLVLTLPAHSVEWIVLEGPTESEFDPIPAIDYWNGAARKARRPFQPPYGQRKCREEPEESEGEENQEFKDYASIYLVFTLPHFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CPL8</accession>
    <name>A0A9Q1CPL8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">UDP-D-xylose:L-fucose alpha-1,3-D-xylosyltransferase 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_00440</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CPL8"/>
    <dbReference type="OrthoDB" id="1712432at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005069">
        <property type="entry name" value="Nucl-diP-sugar_transferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052636">
        <property type="entry name" value="UDP-D-xylose:L-fucose_XylT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47032">
        <property type="entry name" value="UDP-D-XYLOSE:L-FUCOSE ALPHA-1,3-D-XYLOSYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47032:SF1">
        <property type="entry name" value="UDP-D-XYLOSE:L-FUCOSE ALPHA-1,3-D-XYLOSYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03407">
        <property type="entry name" value="Nucleotid_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleotide-diphospho-sugar transferase" evidence="1">
        <location>
            <begin position="28"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8048214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="236" mass="28092" checksum="ECDA66EBF2263A8D" modified="2023-09-13" version="1">MLTATNKDFINITENFLEGLRRLRVRYNITLMAEDEEAYNYFSRRSNSEFHVVSSNQFILPGKLNRNISSYQHLIRRRTVYILTLLRSGRDVLLVDVDAVWLKDPLALIFPDYQKFDAWIAQGKEPGFPCPCFFYMKSVTRMINMVYDWIARVSYFEDGRPVTDQVALFNILEKNRRGVHIKYLDYERFPIGKQYFNWTWHKENHNSVYVAHGNHLGREQGKIETFKEFGVWLLDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CQX8</accession>
    <name>A0A9Q1CQX8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_02624</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CQX8"/>
    <dbReference type="OrthoDB" id="5958414at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1820">
        <property type="entry name" value="L1 transposable element, RRM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35555">
        <property type="entry name" value="ENDONUCLEASE-REVERSE TRANSCRIPTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35555:SF3">
        <property type="entry name" value="ENDONUCLEASE-REVERSE TRANSCRIPTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="66"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8049743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="261" mass="29885" checksum="08870371D579592D" modified="2023-09-13" version="1">MSSPKKRGRPKGSLGKKKKAELSRQATLVSLRSSRTHTLTPSMEEEYEDVAEDTLSQFTDTLDAAIEKITKELKKIRSDFSRAIAEQKKAIEAVKAENVQLKEKCHALETRISGLEKSREEHSALHNKHERFSRRNNIRIVGFPTGPQENCLQMAKDVIAKVGIPDCHLERAHRDGRKINGRDSHILVKLTYFQDKVFVMKNARQALTNEGYYIVEDLTKIDLAEKRRHSRQVSELFQQGVRLRFSGGLWRDGRGKPYAFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CR00</accession>
    <name>A0A9Q1CR00_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_01860</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CR00"/>
    <dbReference type="OrthoDB" id="6783964at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33480">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33480:SF1">
        <property type="entry name" value="TYR RECOMBINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8049220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="288" mass="32654" checksum="3F54BEB0BB874ED6" modified="2023-09-13" version="1">MERLLHQGNFTHNRFVLTEGKGEVIPVRRPDEETAAEAYSPCPPSLNTLSQRKFGKSNELPDAEGIMKFSSFLSREINKSFHDLEGINEIEERSKKYRRAQSVVLAKLAFFNKRRPGEAEQLTGKRGRKVPLMIPKDCDALMNKLIVLRNSAGISKKKKKFGRFGAVTPIRATDSIRELTQEAGLNSPQLIRLTKVRKYVATMSQVFALNEGHTEWLADRLGHSVRVHKEHYRLPNTILEKAKIAKLLLAIDSGMASSFAGKTLEEITFDGKLMFLRFSWSSTSFLYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CR59</accession>
    <name>A0A9Q1CR59_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Retrotransposon gag domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_02337</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CR59"/>
    <dbReference type="OrthoDB" id="10070970at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="218"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="218"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="249"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8049551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="314" mass="36487" checksum="1A5C47E19D4162D6" modified="2023-09-13" version="1">MANDNLRTTDFLPSRFKGETINSDLARAHFLAFQDYLDAHNIAQPEDNAALLRITNTFKRTLEGQARLWIEGKQFQSFQELRTRFLARFSPSNSHFAKSQEFQALTYTAGDTAEVHLAKISKAAHMLQYNDDQIRDKFLSSLTTQCRSAVLMAAPMDAPLPLLVDKAQCYFDLQSSQNSTAIAVAADVMTTQDMREPTPQDKTIQSDMQDIVHRMEALESKFTKSEQPNEKSRSSRSRNREQRGNFAQNRRRSNNRFSNQRRRFNSIICDFCSRQGHTWRNCFERLNQTAQSYAQYEQNDFWQAPCQRTYQNFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CR82</accession>
    <name>A0A9Q1CR82_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_02045</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CR82"/>
    <dbReference type="OrthoDB" id="5987933at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8049340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="86" mass="9570" checksum="5D4201AB550F5ECB" modified="2023-09-13" version="1">MLQALEARNTYDLKFTSDAARVKGVSCLKGVEGLTVTPYDGSVAVTVLYLPFEVDQRLVARVLSQYGTVSEMRWCKYTVGELKGIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CRC6</accession>
    <name>A0A9Q1CRC6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Leucine-rich repeat-containing protein 15</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_03244</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CRC6"/>
    <dbReference type="OrthoDB" id="694479at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001360">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050541">
        <property type="entry name" value="LRR_TM_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369">
        <property type="entry name" value="ANTIGEN BSP, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369:SF210">
        <property type="entry name" value="CHAOPTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040338768" evidence="5">
        <location>
            <begin position="25"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="466"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="domain" description="LRRCT" evidence="6">
        <location>
            <begin position="393"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8050152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="490" mass="56073" checksum="17B3DDBE8EAB8F5F" modified="2023-09-13" version="1">MLHNAVLFISLVSRILISHQACESHPDFYDECENPFPSGCCTLGTAFICNGANLTDVNHFADEIPQTCTSLTVANTNGSFINNGDWKFLSNLTNLEELSFFSQKMSHLWDLKPFYLKDLVKLKVLTLAYGDLHKFPVEKFTNLMSLMYLNLRCNKFQSIGEGNWNFSSLNTLVLTENRITQVTSEQFRNLDNLSTLDLSHNRIYFFSLKVLDSMPNLKHVKLSFNRLTHVSDYRLQHVNVKTFTLNSNLFASLEPFVFNGLKSLQNIDYRNNSIKEPPMINSQNSVPSALRLSLQFNVIEILSPQFFDNFPHLKNINVADNRIRHIEEDAFRSVPNLTVINMRINEIDTIPRQLFQHLGNLVIVELQFNRIQVLDVHVLTPLPRYVKIYIHDNPIVCDCQVIPLAKWLSLSATPLNNFPTCQSPEDLQNQNIYYAKLPQDCPHSTQKPVTTSIIPTSYPETHTNHYILGLVIGALTLVMFISLIYFSIGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CSW5</accession>
    <name>A0A9Q1CSW5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Sushi domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_04393</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CSW5"/>
    <dbReference type="OrthoDB" id="6103690at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0768">Sushi</keyword>
    <feature type="domain" description="Sushi" evidence="4">
        <location>
            <begin position="57"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8050992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="185" mass="20880" checksum="C50736C2B689DDD0" modified="2023-09-13" version="1">MEFVLQTEEDSNHHQCGRSLVITAPTQWPWLLQNFDDIGGDKDAEEARKNSHPYEKDSCKPELTHPTHGSVFCSNGNKSESVCQYTCDEGYQLSDPESSVLICKGKNWSGKESVCVADKALRQLVKLIFQVGFTSKSYFKVESCTFGQTSQIGRGHTVKKKVPFNETSWIVLLIGYFHDYLSIEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CTS0</accession>
    <name>A0A9Q1CTS0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Lipoxygenase-likey domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_03558</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CTS0"/>
    <dbReference type="OrthoDB" id="5322100at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01756">
        <property type="entry name" value="PLAT_repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.180.10">
        <property type="entry name" value="Catalase core domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052970">
        <property type="entry name" value="Inner_ear_hair_cell_LOXHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001024">
        <property type="entry name" value="PLAT/LH2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036392">
        <property type="entry name" value="PLAT/LH2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45901:SF3">
        <property type="entry name" value="LIPOXYGENASE HOMOLOGY DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45901">
        <property type="entry name" value="PROTEIN CBG12474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01477">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00308">
        <property type="entry name" value="LH2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49723">
        <property type="entry name" value="Lipase/lipooxygenase domain (PLAT/LH2 domain)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50095">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PLAT" evidence="3">
        <location>
            <begin position="170"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="PLAT" evidence="3">
        <location>
            <begin position="304"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="PLAT" evidence="3">
        <location>
            <begin position="435"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="141"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8050374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="561" mass="62741" checksum="A167036EC56BD25C" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H1W9</accession>
    <name>A0A9Q1H1W9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SCAN box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_28358</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H1W9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF13">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCAN box" evidence="2">
        <location>
            <begin position="231"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="325"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="589"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="689"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="325"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="340"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="364"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="593"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="633"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="658"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="705"/>
            <end position="731"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8029056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="764" mass="86299" checksum="BE4BF853869B52CB" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H2E2</accession>
    <name>A0A9Q1H2E2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Cell adhesion molecule-related/down-regulated by oncogenes</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_29086</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H2E2"/>
    <dbReference type="OrthoDB" id="10654359at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040196109" evidence="3">
        <location>
            <begin position="24"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="346"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="127"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="563"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="600"/>
            <end position="625"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8029641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="643" mass="71613" checksum="1329E1ECAD494445" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H4M1</accession>
    <name>A0A9Q1H4M1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Lachesin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_22410</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10012075at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013162">
        <property type="entry name" value="CD80_C2-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051275">
        <property type="entry name" value="Cell_adhesion_signaling"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11640:SF155">
        <property type="entry name" value="IG-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11640">
        <property type="entry name" value="NEPHRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08205">
        <property type="entry name" value="C2-set_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="4 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040325385" evidence="10">
        <location>
            <begin position="23"/>
            <end position="1308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="438"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="820"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="36"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="129"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="327"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="491"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="959"/>
            <end position="1051"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="1072"/>
            <end position="1164"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="1169"/>
            <end position="1268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="919"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="920"/>
            <end position="936"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8035247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1308" mass="144129" checksum="734B9C25AF41EB62" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H664</accession>
    <name>A0A9Q1H664_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein split ends</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_20784</name>
        <name evidence="2" type="ORF">HOLleu_24260</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H664"/>
    <dbReference type="OrthoDB" id="6142091at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8033885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8036726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="159" mass="19358" checksum="15A0D4364042CFBE" modified="2023-09-13" version="1">MPFNLDKFVASPSVEELDSLKKSDIVKVAKHYGIEFQPLMRKDEIKRYVLEYLVDESILPSTVLETAITVPTDNTFELKRLEMEMNKEIRLKEMEREREREERERKEREMQMQKEKEEREMQMQMQKEKEEREMQMQREKEAREHEFRARKICPQISGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H6Z9</accession>
    <name>A0A9Q1H6Z9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Fibroblast growth factor receptor 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_22211</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H6Z9"/>
    <dbReference type="OrthoDB" id="1915767at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004714">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050122">
        <property type="entry name" value="RTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416:SF611">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE TRANSMEMBRANE RECEPTOR ROR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="80"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="166"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="324"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR000615-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8035098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="471" mass="52993" checksum="25FFAA487D2035C6" modified="2023-09-13" version="1">MSYIPICKVYLSDEMVRCKCDANPKISEYKIYVNGKVKSVNKSLSLADHSPGDFSCSGTNEMGSGNSSSQYFQKDQANTIVGLVCGIAVCCLLVAGLIPVNVYIFKKRRKTMPSLSLASHTPTTRRTSGQPYIMECPQRRIPPRESSTVIGDSVDIYAEVYANAPKDKFVKLNEIYFGKKLKTSNHLNRWKGKLKSIRDVVISSPPENASPITQLQWASFVKNLIDLPKVLHIVDVKGICIEKGNFYLLQEYMRTGTLRKFLVSIQYTSVSRETEFKPTCNILLELASSLVQGIDFILRQQFYHPGLCSDKVLVADGNVCKLYDFCTVDSVKDRIDEIAIKVKEGTSARTLPPEAVFKRMYDKASEMWSLAATVWEIFSYGQKAKFNYLKELSLQDPLQGECKLKKPQNMNNDIYDLMARCWSNQPSSRPKIFQLKQAIDETINGSNGKAKNTDSVLSQDYEPMTGRAKIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H7X7</accession>
    <name>A0A9Q1H7X7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Nascent polypeptide-associated complex subunit alpha</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_19459</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8035701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H7X7"/>
    <dbReference type="OrthoDB" id="3169036at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005854">
        <property type="term" value="C:nascent polypeptide-associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22054">
        <property type="entry name" value="NAC_NACA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14358">
        <property type="entry name" value="UBA_NAC_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.30:FF:000002">
        <property type="entry name" value="Nascent polypeptide-associated complex (NAC), alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000006">
        <property type="entry name" value="Putative nascent polypeptide-associated complex subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.30">
        <property type="entry name" value="Nascent polypeptide-associated complex domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016641">
        <property type="entry name" value="EGD2/NACA0like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044034">
        <property type="entry name" value="NAC-like_UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038187">
        <property type="entry name" value="NAC_A/B_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002715">
        <property type="entry name" value="Nas_poly-pep-assoc_cplx_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21713">
        <property type="entry name" value="NASCENT POLYPEPTIDE ASSOCIATED COMPLEX ALPHA SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01849">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19026">
        <property type="entry name" value="UBA_HYPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015901">
        <property type="entry name" value="NAC_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01407">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51151">
        <property type="entry name" value="NAC_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAC-A/B" evidence="2">
        <location>
            <begin position="72"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="135"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="166"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8035701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="219" mass="23870" checksum="31E5A1D68CB11AC7" modified="2023-09-13" version="1">MPSETTTPGVQATADEPMKDAEAASSGTESDSGSDHDVPELEEHPDHPEHSTPQVQTELGGSEEPVSKTKQSRSEKRARKELNKLGLKSITGVSRVTIRKSKNILFVITKPDVYKSPASDTYIVFGEAKIEDISQQAQMQAAEQFRPQEPPNQVASESEASVNPVAEEESDDEEVDEAGLEDKDIEIVKQQANVSRGKAVKALKNNNNDIVNAIMELTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H9E7</accession>
    <name>A0A9Q1H9E7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">ATP-binding cassette sub-family A member 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HOLleu_17904</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Nanhai2018</strain>
            <tissue evidence="15">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>cholesterol(in) + ATP + H2O = cholesterol(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:39051"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16113"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:39052"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="6512918at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005319">
        <property type="term" value="F:lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03263">
        <property type="entry name" value="ABC_subfamily_A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000327">
        <property type="entry name" value="ATP-binding cassette sub-family A member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000465">
        <property type="entry name" value="ATP-binding cassette, sub-family A (ABC1), member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026082">
        <property type="entry name" value="ABCA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056264">
        <property type="entry name" value="R2_ABCA1-4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229:SF250">
        <property type="entry name" value="ABC TRANSPORTER DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER SUBFAMILY A ABCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12698">
        <property type="entry name" value="ABC2_membrane_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23321">
        <property type="entry name" value="R1_ABCA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="520"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="700"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="750"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="782"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="814"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="852"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="912"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="14">
        <location>
            <begin position="109"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="14">
        <location>
            <begin position="990"/>
            <end position="1225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="457"/>
            <end position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00050894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ8037156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1311" mass="146798" checksum="E12BD79DC9AB0F9D" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H9Q4</accession>
    <name>A0A9Q1H9Q4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_18526</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H9Q4"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8037648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="85" mass="9690" checksum="56DF0E87C6ABD336" modified="2023-09-13" version="1">MTMGEFGRENALSHDLRIFDSSANILFFLFLFMMPLVLVNLMVSGRINVYVAGNLKHTGPLSFGAEHLSPNFKHVSRKQYIFARK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HA48</accession>
    <name>A0A9Q1HA48_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Zinc finger MYM-type protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_19059</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8038083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HA48"/>
    <dbReference type="OrthoDB" id="6629565at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052958">
        <property type="entry name" value="IFN-induced_PKR_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46289">
        <property type="entry name" value="52 KDA REPRESSOR OF THE INHIBITOR OF THE PROTEIN KINASE-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46289:SF17">
        <property type="entry name" value="HAT C-TERMINAL DIMERISATION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8038083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="276" mass="31041" checksum="F91725AC9425BC97" modified="2023-09-13" version="1">MQTDEIHISASKLDLGSSKHQWRGVFYVTNMEESGYINVLAKEDDPIFSRRSHLQCPNIFTCSGINSVEVRVMKLKKTLPEVIDTLEEIQESETDAQISSTAAGLLNCVQKFEFSAILCLILDLLETTRVASDALQARGITLDRATQLIDDTITGVREKRTDAYFEKMINSTVELADGAGASTDFERLRPHHRKVLPGELAQDEVMSACDRLKANVFHMAMDTFLVQLEECFVENRKILSSFKILTPLLIVERSIPKEKINSLVECEKILGTVKQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HDH3</accession>
    <name>A0A9Q1HDH3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Extracellular tyrosine-protein kinase PKDCC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_08443</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HDH3"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004715">
        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001501">
        <property type="term" value="P:skeletal system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042983">
        <property type="entry name" value="PKDCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46448:SF4">
        <property type="entry name" value="EXTRACELLULAR TYROSINE-PROTEIN KINASE PKDCC-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46448">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="142"/>
            <end position="519"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8045432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="519" mass="59039" checksum="3BC6DFEE968E388F" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HHR9</accession>
    <name>A0A9Q1HHR9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Fucosyltransferase</fullName>
            <ecNumber evidence="11">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_05998</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Endomembrane system</location>
            <topology evidence="10">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Golgi apparatus</location>
            <location evidence="11">Golgi stack membrane</location>
            <topology evidence="11">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the glycosyltransferase 10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="11"/>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HHR9"/>
    <dbReference type="OrthoDB" id="427096at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046920">
        <property type="term" value="F:alpha-(1-&gt;3)-fucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11660:FF:000002">
        <property type="entry name" value="Alpha-(1,3)-fucosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11660">
        <property type="entry name" value="Glycosyl transferase family 10, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055270">
        <property type="entry name" value="Glyco_tran_10_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031481">
        <property type="entry name" value="Glyco_tran_10_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001503">
        <property type="entry name" value="Glyco_trans_10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038577">
        <property type="entry name" value="GT10-like_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11929">
        <property type="entry name" value="ALPHA- 1,3 -FUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11929:SF145">
        <property type="entry name" value="ALPHA-(1,3)-FUCOSYLTRANSFERASE FUT-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17039">
        <property type="entry name" value="Glyco_tran_10_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00852">
        <property type="entry name" value="Glyco_transf_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="domain" description="Fucosyltransferase N-terminal" evidence="13">
        <location>
            <begin position="101"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="Fucosyltransferase C-terminal" evidence="12">
        <location>
            <begin position="213"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00060399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF17039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ8047099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="425" mass="50286" checksum="DE6E60DD81054B88" modified="2023-09-13" version="1">MSTRDSIPFLTYIFRLLAILSVLTTLVFIVCCIYLQMNVQTIKHSPRIESSIFEDYSYYENLQIPSLEENRAQIISLIRLLVYGFEFYWSRDSLKEERDVLCPEINTTVKVKFSSDHRAIYHADITFFSHLLRLGKMRTWKDHLDRRRKRQRWALFSLEPPIRSARNTTVHWGWRYRTYHWTATYNAESDIPFPYGKFVKDSSYQNESTELYNSKPKLLAFLSSNCNMTKWPRLRFVQTLQKYVPIDMYGKCGTIPCDWQRGCENDLKQYKFFLALENSQCEGYITEKFWNSLSRYKSVPIVWGARQHDYELVAPSHSYIHVSQFKSIKQLARRINQIGSNEVEYNSYHIWRKSGHIELLSDWSTLPSDGHVCAAAKRYQEDMIKLAKTNKTSFRDVNGKDWLESCKVGKDQVERKLPIPEIQGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HIC0</accession>
    <name>A0A9Q1HIC0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Uridine diphosphate glucose pyrophosphatase NUDT14</fullName>
            <ecNumber evidence="9">3.6.1.45</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Nucleoside diphosphate-linked moiety X motif 14</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_09010</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Hydrolyzes UDP-glucose to glucose 1-phosphate and UMP and ADP-ribose to ribose 5-phosphate and AMP. The physiological substrate is probably UDP-glucose. Poor activity on other substrates such as ADP-glucose, CDP-glucose, GDP-glucose and GDP-mannose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>UDP-sugar + H2O = UMP + alpha-D-aldose 1-phosphate.</text>
            <dbReference type="EC" id="3.6.1.45"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.45" evidence="9"/>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HIC0"/>
    <dbReference type="OrthoDB" id="10249920at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008768">
        <property type="term" value="F:UDP-sugar diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006753">
        <property type="term" value="P:nucleoside phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019693">
        <property type="term" value="P:ribose phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18887">
        <property type="entry name" value="NUDIX_UGPPase_Nudt14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.79.10:FF:000035">
        <property type="entry name" value="Uridine diphosphate glucose pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004385">
        <property type="entry name" value="NDP_pyrophosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00052">
        <property type="entry name" value="nudix-type nucleoside diphosphatase, YffH/AdpP family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11839">
        <property type="entry name" value="UDP/ADP-SUGAR PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11839:SF15">
        <property type="entry name" value="URIDINE DIPHOSPHATE GLUCOSE PYROPHOSPHATASE NUDT14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="12">
        <location>
            <begin position="44"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00051086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00054674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00066480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00071467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00080475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8045898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="216" mass="23995" checksum="E9539F26C2EAFFB8" modified="2023-09-13" version="1">MVNKNNAAKISDAHVVSCHSSNYVKPLRLEYLQNGLTKKWDYLRVHDSVAILLFNTDRDVFILVRQFRPAVYASKAQAHTVGQTVDMTSFPGSLGMTFELCAGIVDKDIGLQETAKAEILEETGYDVPLDKIKRMTKFRSSVGITGSSQTLFYAEVTDSMKASTGGGIAEEGERIEVIELPLKEANDFIFDENIEKPGGLIAAFLWYFRERGITSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HJ09</accession>
    <name>A0A9Q1HJ09_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">RNA-directed DNA polymerase from mobile element jockey</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_01040</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HJ09"/>
    <dbReference type="OrthoDB" id="6771564at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33273">
        <property type="entry name" value="DOMAIN-CONTAINING PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33273:SF4">
        <property type="entry name" value="ENDONUCLEASE_EXONUCLEASE_PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14529">
        <property type="entry name" value="Exo_endo_phos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="2">
        <location>
            <begin position="262"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="128"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="146"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="178"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8048644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="537" mass="60599" checksum="C767788F732E37D4" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HM48</accession>
    <name>A0A9Q1HM48_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nucleoporin NUP35</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">35 kDa nucleoporin</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Nucleoporin NUP53</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HOLleu_04079</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Nanhai2018</strain>
            <tissue evidence="15">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Nup35 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HM48"/>
    <dbReference type="OrthoDB" id="3365060at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044613">
        <property type="term" value="C:nuclear pore central transport channel"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044615">
        <property type="term" value="C:nuclear pore nuclear basket"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006607">
        <property type="term" value="P:NLS-bearing protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006999">
        <property type="term" value="P:nuclear pore organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12722">
        <property type="entry name" value="RRM_Nup53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000095">
        <property type="entry name" value="Putative Nucleoporin NUP53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017389">
        <property type="entry name" value="Nucleoporin_NUP53"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007846">
        <property type="entry name" value="RRM_NUP35_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21527">
        <property type="entry name" value="NUCLEOPORIN NUP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21527:SF6">
        <property type="entry name" value="NUCLEOPORIN NUP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05172">
        <property type="entry name" value="RRM_Nup35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038119">
        <property type="entry name" value="Nucleoporin_NUP53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51472">
        <property type="entry name" value="RRM_NUP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="8 12" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="9 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 12" id="KW-0813">Transport</keyword>
    <feature type="domain" description="RRM Nup35-type" evidence="14">
        <location>
            <begin position="217"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="146"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="18"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="52"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="146"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ8050761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="373" mass="39714" checksum="47CD7F96B279062B" modified="2023-09-13" version="1">MNFHGPSSPGAEPMSLGSPPQSQNLHQSLLSHPVSQSSGVLPGYLMGDPGHSPSSSRLWSSSRSPGKVIAPSSLGPKDSTSRLDRSDSILSQSRGGINPAAKDHGNAPPVAGLYDQVDSPHGLGVATHHIGTPERRQLEFPASAVTGGSLSRQTSEATPRQLQSQVSVGPPTLSRSFSHTHTPQGRSIGLMSPPSVASPAQIDPFYTQGESIVPDVELDQTWVTVFGFPPAAASYILQQFSQYGNIQKHVVASNGNWMHLQFSSKLQAKKALSKNGKIFGGNIMIGATPCIDKGVMEGGKENLYSTNLAGTPTNKRTLSITEEPDLTKRKTPIRPLTAAYQAVSSNHEVLPDHNTPQKSSTVLSKTLEYMFGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YBD8</accession>
    <name>A0A9Q0YBD8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_41302</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8019642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YBD8"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8019642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="124" mass="13856" checksum="6C52C3349E93B1CD" modified="2023-09-13" version="1">MAHIERKNPIVFGGGQRSSGVTGGQNLKTLFTQYLKIRCGERKNPIIFGGGQRSSGVTGGQNLKTLFTQYLKMRCGERKNPIVFGGGQRSSGVTRGQNLKTLFTQYLKLESLDRFHAWYVDALW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YBL9</accession>
    <name>A0A9Q0YBL9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Tenascin-R</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_41502</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8019777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YBL9"/>
    <dbReference type="OrthoDB" id="6145874at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050373">
        <property type="entry name" value="Fibrinogen_C-term_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143:SF458">
        <property type="entry name" value="FIBRINOGEN C-TERMINAL DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143">
        <property type="entry name" value="FIBRINOGEN/TENASCIN/ANGIOPOEITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00186">
        <property type="entry name" value="FBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8019777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="177" mass="21018" checksum="0ED1C3A061B58D13" modified="2023-09-13" version="1">MSIDGGGWTVLQRRVNGSVSFEHNWEEYKNGFGILEHELWLGNNKMHYITNRKNYELRMDMINLDEDAYFAKYSLFRIKDENFKFQLEIGTYDSASTASYDAMDYHRDQFFSTPDMDNDAHSGNCAANYRAGWWYKSCNHFDFNAYDFRSCSGYHHYWYNLPGSNCLMYTDIKIRPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YCJ2</accession>
    <name>A0A9Q0YCJ2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_42719</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000125">
        <property type="protein sequence ID" value="KAJ8018971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YCJ2"/>
    <dbReference type="OrthoDB" id="10210969at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33480">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33480:SF1">
        <property type="entry name" value="TYR RECOMBINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="178"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8018971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="199" mass="22957" checksum="6A4076F6F81C81FC" modified="2023-09-13" version="1">MSVSSCKCQHVRESLALLPVKEFVSKQMKGILDSMTQDDIALMIQNDYMLRFGEHFISKAGHKSHSERYIAQKMRELGRLQEFHKITQTPMACFDLVNPLRFDKVIEATKECSGFNSEDNTFQIPSLALKTGHNLNKCEIVLAESIKKKGSEVQHAARECQELMTIEWARKISSHALRQRDKQQWQNPPKKLQQKIPFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YCL6</accession>
    <name>A0A9Q0YCL6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Helix-turn-helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_39712</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YCL6"/>
    <dbReference type="OrthoDB" id="10063818at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058912">
        <property type="entry name" value="HTH_animal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26215">
        <property type="entry name" value="HTH_animal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helix-turn-helix" evidence="1">
        <location>
            <begin position="8"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF26215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8020188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="198" mass="22739" checksum="828CA5EA6719371A" modified="2023-09-13" version="1">MKPNNIPLYVHKQSNHPPLIIKNIPESINRRLSNISSDENTFNTSAPIYQEALHKSGYGYNLKYRTQPRNTSQQNKATKRRHARNITWFNPPFSDNVTTNIGKKFFNLLDSCFPPGHKLHTLLNRNTVKLSYSCMANMDQIISAHNKYILNKQNLQPATQNNCNCRTQSQCPLQGNCLTNNIVYQATVTRHDNQLTLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YDL3</accession>
    <name>A0A9Q0YDL3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">IgGFc-binding protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_43605</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000379">
        <property type="protein sequence ID" value="KAJ8018419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YDL3"/>
    <dbReference type="OrthoDB" id="10005154at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035234">
        <property type="entry name" value="IgGFc-bd_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46534:SF1">
        <property type="entry name" value="IGGFC-BINDING PROTEIN N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46534">
        <property type="entry name" value="IGGFC_BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17517">
        <property type="entry name" value="IgGFc_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IgGFc-binding protein N-terminal" evidence="1">
        <location>
            <begin position="171"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF17517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8018419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="345" mass="38853" checksum="FB9C514D5F460545" modified="2023-09-13" version="1">MFFILLSGHCEKPICYVVDEIDPRNRHIYKRDCNHTDEIKLDKIIRYSCASDIHIGASKEIPEKFIFTFFAVNCNEPTTEIFIGTKFSDMGTSGNISFPLIDIPNKTFSLYPGSGETFEVPLTAVSSESELNQKVNTAVVIASDEEVVVYCHHRCHGSNNQRATAFRVRATVDLGTEYWVITHQSGGKSQVGVAATKDHTNISFNGVYINRTLNLNKYEIFYFGGSYDLAGTYIVADKPIFVIAGNSADTSPNPKTNHIDSFNKCLPPIKNWGRHFTLVPFPPQSQPFTAKILPSHDNCKLAYVDGTGSHNFGFIEQRRSKSFKFFNCTERKFIRGDPRSSIFKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YF74</accession>
    <name>A0A9Q0YF74_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">dual-specificity kinase</fullName>
            <ecNumber evidence="2">2.7.12.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HOLleu_40465</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Nanhai2018</strain>
            <tissue evidence="16">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Lammer subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.12.1" evidence="2"/>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YF74"/>
    <dbReference type="OrthoDB" id="283111at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004712">
        <property type="term" value="F:protein serine/threonine/tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043484">
        <property type="term" value="P:regulation of RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14134">
        <property type="entry name" value="PKc_CLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000061">
        <property type="entry name" value="Dual specificity protein kinase CLK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051175">
        <property type="entry name" value="CLK_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45646">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE DOA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45646:SF11">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE DOA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="139"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="75"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="27"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="52"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="82"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="110"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ8020784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="477" mass="56819" checksum="F2B1FA912AC10155" modified="2023-09-13" version="1">MTMPRYHRRSRSRSHSYHHRHRRRRTYSRDRDVYREERYQDSKKYRSRSRHRDRDRSSSRRGYRDSRYYEEYRGDCHDRNHSRGYHRSKRRHHRKRSREHSYSTASSKSLKTDGRRQSVVDDDEGHLVYRPGDTLQARYEILSTLGEGTFGKVVQCKDHSQGPHDKVALKIIKNIEKYREASKLEINVLEELKRNDPEGRYLCVKMLDWFDYHGHMCLVFEKLGSSIFDFLVSNQAVATLFIKSEEIVFGVCSPMSTVMHDMKLTHTDLKPENVLFVNSDYVLEYNPVKRREERLVKDSTIRLIDFGSATFDFEHHSTIVSTRHYRALEVILELGWSQPCDVWSIGGIIFELFTGFTLFQTHDNREHLAMMETILGPMPAWMVKKSKKTKYFYRGDVDWDRNSSSGRYVRENCRPLMEYKTSDSEEQNQLFDLIELMLNYDPNERISLKQALEHPFFDALPPHLRIHDSRASHSISR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YG46</accession>
    <name>A0A9Q0YG46_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Sodium-coupled monocarboxylate transporter 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_39123</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YG46"/>
    <dbReference type="OrthoDB" id="6132759at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="entry name" value="Sodium/glucose cotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051163">
        <property type="entry name" value="Sodium:Solute_Symporter_SSF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42985:SF40">
        <property type="entry name" value="LD47995P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42985">
        <property type="entry name" value="SODIUM-COUPLED MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="10" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="56"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8021820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="150" mass="16504" checksum="9857E25631B63ED1" modified="2023-09-13" version="1">MSSGEEIVGKFDAADYVILSLCLGVSASIGIYHAFAGGGQKTAEEFLLADRSMHPFPVSVSLLASFISAITVLGTPAETYVQGTMYWLFSFAYIGVGIWTSRVFIPVFYKYKITSVNEVSQFVILDQSCNLVNINMINKTSRLQTFTSWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YKT8</accession>
    <name>A0A9Q0YKT8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_39406</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8022032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YKT8"/>
    <dbReference type="OrthoDB" id="6138683at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33332">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8022032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="274" mass="32189" checksum="C8981B57AA4DEDEF" modified="2023-09-13" version="1">MDDIENLQNDLDQLQSWSDKWLLKFHPDKCKVLTVSLNRYHDCLPRSYHLYGDSGDEVFLEKSDCEKDIGVLVDSGLRFSEHIQHQVNKANSIMGRTRRTYTFLDEQCFKLLFQALVRPHLEYAAAVWSPHYQRDIDAIENVQRRATKLIPSLKGLTYTERLKKLKLPTLKYRRLRGDMIEVFKIVSGVYDRGVTEGFLKFSSITHTRGHSRKLLKEFCRLNIRKFSFVCRVVDIWNSLPESVVSATTVLQFEKALDRFWAGHEMLYNYKADFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YLF7</accession>
    <name>A0A9Q0YLF7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Carbohydrate sulfotransferase</fullName>
            <ecNumber evidence="9">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_37118</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Golgi apparatus membrane</location>
            <topology evidence="1 9">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the sulfotransferase 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="9"/>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YLF7"/>
    <dbReference type="OrthoDB" id="2019940at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016051">
        <property type="term" value="P:carbohydrate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018011">
        <property type="entry name" value="Carb_sulfotrans_8-10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005331">
        <property type="entry name" value="Sulfotransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12137">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12137:SF54">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03567">
        <property type="entry name" value="Sulfotransfer_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="8 9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8022272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="361" mass="42775" checksum="58B877FD142A2953" modified="2023-09-13" version="1">MEVHGYKTPQNDCSRRIREGLLLSEKICLMLILSACSTGYYIVHTSTWQRVYKKITIPHPVQEYSPSAVEEWYQSNYTTSRGSESTRPQEGNFANTKWIQEMEHSQRWRKNVTDQVCLKHRIFQQSVDAFLSFRGNRGTLFANDQHKVIFSKIHKVASNNFTKLWKTLGGLKHLYKESRDLCVGRIERYYKVIFVRHPLSRILSAYRDKFERHNYETFRKIAEGIKRRQRVKFPKAAKNTSSLAFEEFIHYLVNANKNRENSHWNLISSSARVCELRYHFIGKLESLDNDLQYLLHKLRSNASLTILRAYQGYNGEVETLRHYYGNISRPLLRRLYQAYKMDFDMFGYDMTILGSKIWPTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YM52</accession>
    <name>A0A9Q0YM52_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_37626</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YM52"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8022662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="113" mass="12766" checksum="2DC9BA27CCACCC0B" modified="2023-09-13" version="1">MVVFCSHLHSQTVKQPLNLASTKIILLTSFRNTTFCQLQDTKMFSSRVLFLLVLFGAVAIIASAPSRFENDEIDDLVARVAALDPDVRSFWKHLATHAHHYLSNYLGHEEHAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YN00</accession>
    <name>A0A9Q0YN00_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein FAM184A/B N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_33007</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YN00"/>
    <dbReference type="OrthoDB" id="75801at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039478">
        <property type="entry name" value="FAM184A/B_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18870">
        <property type="entry name" value="PROTEIN TAG-278-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18870:SF9">
        <property type="entry name" value="PROTEIN TAG-278-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15665">
        <property type="entry name" value="FAM184"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein FAM184A/B N-terminal" evidence="4">
        <location>
            <begin position="48"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="322"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="411"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="458"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="856"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="915"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1038"/>
            <end position="1116"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="56"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="156"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="637"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="323"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="334"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="417"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="466"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="915"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1048"/>
            <end position="1060"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1061"/>
            <end position="1073"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF15665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8025448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1116" mass="129993" checksum="14B91820BDB1D735" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YN77</accession>
    <name>A0A9Q0YN77_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Beta-1,3-glucan-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_33203</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YN77"/>
    <dbReference type="OrthoDB" id="4781at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="Beta-glucanase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050546">
        <property type="entry name" value="Glycosyl_Hydrlase_16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10963:SF55">
        <property type="entry name" value="GLYCOSIDE HYDROLASE FAMILY 16 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10963">
        <property type="entry name" value="GLYCOSYL HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00722">
        <property type="entry name" value="Glyco_hydro_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GH16" evidence="2">
        <location>
            <begin position="1"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8025603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="247" mass="28205" checksum="EF9CFD4D6DDE32E4" modified="2023-09-13" version="1">MVAMAWQLSEQSNTGFMMPLGCFNQFIVQLRSMSTTVTIVISKFNAITPISCVRDAPASSKLQRNEDLKNPSETYVGIQQMGQTLHWGPYLPLNGSPKTHVTKNLPDDEKFSDAYHQYGLEWTADSLIFYLDDEETMTVDPGDQGFWEYGRFGYRLGIDNPWANSPNKLAPFDQEFYIIINVAVGGIRYFADNLTNKPYPKPWLNNSTHPIKDFYDAKDYWHPTWNAEVNNGEDAALKVRSVRVWAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YQR0</accession>
    <name>A0A9Q0YQR0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">G-protein coupled receptor moody</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_31642</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YQR0"/>
    <dbReference type="OrthoDB" id="9996086at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00637">
        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24228">
        <property type="entry name" value="B2 BRADYKININ RECEPTOR/ANGIOTENSIN II RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24228:SF74">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="23"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="60"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="197"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="10">
        <location>
            <begin position="39"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8026724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="240" mass="26465" checksum="A404CC4AE7FAAABD" modified="2023-09-13" version="1">MMDVATSSEGNISGFIPPTPEHYVLGGVYALISVLGMAGNFLVILAVWRHRSLQNSTNAFIVNLAFADLLTSALLPIYVASFWTTTYTSSMDSFCALSTGLLRAFVSCSTLSLAAISINRLVIVTQAKRTYRWLFTRRRIVYSIFVMWIYGILLSFSTHFTGFVRLGFDVTIHQCGVVPISPDSLAIGHGSHSIGTFPLQFFIVLVCYAWIYLFVFRHNRRMQKHAESEPAGAISGAGGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YQR6</accession>
    <name>A0A9Q0YQR6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Fatty acid synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_31923</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YQR6"/>
    <dbReference type="OrthoDB" id="329835at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004312">
        <property type="term" value="F:fatty acid synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.366.10">
        <property type="entry name" value="Malonyl-Coenzyme A Acyl Carrier Protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001227">
        <property type="entry name" value="Ac_transferase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014043">
        <property type="entry name" value="Acyl_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050091">
        <property type="entry name" value="PKS_NRPS_Biosynth_Enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43775">
        <property type="entry name" value="FATTY ACID SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43775:SF7">
        <property type="entry name" value="FATTY ACID SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00698">
        <property type="entry name" value="Acyl_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="3" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="1" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Malonyl-CoA:ACP transacylase (MAT)" evidence="9">
        <location>
            <begin position="8"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8026948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="172" mass="19420" checksum="75DB86422108473E" modified="2023-09-13" version="1">MYCITQIISHQALRSKSWLSTSYPEELWEKPEAMWLSTSYLVNNLVSPVLFHDAVCKIPPSAIVIEIAPHGSMKVLLQRTIPSDCDYLSLMNMKEPDNLHYFLSAVGKLFSMGISLDISKLYPLIKYPVGTGTPPLSPGIKWDHSTEWAVPSWEDFILDSSGDRATIWYEIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YS03</accession>
    <name>A0A9Q0YS03_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_33398</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YS03"/>
    <dbReference type="OrthoDB" id="5981473at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028043">
        <property type="entry name" value="PAAT-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14787:SF1">
        <property type="entry name" value="ATPASE PAAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14787">
        <property type="entry name" value="C10ORF188 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14958">
        <property type="entry name" value="PAAT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="205"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="313"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="271"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="205"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="221"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="331"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8025754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="347" mass="39037" checksum="0D986A58CD6D3ED5" modified="2023-09-13" version="1">MEIYDSKGEYLMTVRGQKEEDGQEMLYRKCLTFESSLPACSLKLLSLASKGCLKLYSLQTSVRRVDPVPTHGPKRQIDMVKVREMLSSMSAPVPENVQHLMQTVEQYQENQSSVWSDIQQQVADSAVKNTSTAPMAGLLSMLARANDMSRLAGRESTDSNTVTDAMSKMMSSVCGGQVAGQTSGEDLYNSLRGICRNVSQMRVDEKREMRKGREDEEEMGEKESAGMEKELQPPLGDPKTAEKLERRLVAQLEALEERMMGRLERRLDAIETHLELRLNRIEAIVENLSQKCVERQKRSENLARRENQGNILQRLADAKEDELDVSREETTSGTQFDDSVSDTGQSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YTN3</accession>
    <name>A0A9Q0YTN3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_32203</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YTN3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="22"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8027149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="193" mass="21020" checksum="6622FF179EC14402" modified="2023-09-13" version="1">MDLDSSVQDNPSTSASVRLSPNTNASTSATRTNRDEPSVKKTAAKPKHTENKSKKTPAQKNRSKAAKSVSEPSNEVNNRLDRLEGLLENLISGFYAQDPPGGNQLPDPNQCSFEYTRNTAGVTAEPADQDTVISEDNLPTVDNDITDPEAGTSEQNIGFASRFAARNDEPVMMRVKIFHLSWQTALISLSLRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YTQ5</accession>
    <name>A0A9Q0YTQ5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">EF-hand calcium-binding domain-containing protein 9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_33643</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YTQ5"/>
    <dbReference type="OrthoDB" id="186625at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097228">
        <property type="term" value="C:sperm principal piece"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061891">
        <property type="term" value="F:calcium ion sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030317">
        <property type="term" value="P:flagellated sperm motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042798">
        <property type="entry name" value="EFCAB9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47065">
        <property type="entry name" value="EF-HAND CALCIUM-BINDING DOMAIN-CONTAINING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47065:SF1">
        <property type="entry name" value="EF-HAND CALCIUM-BINDING DOMAIN-CONTAINING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00036">
        <property type="entry name" value="EF-hand_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="27"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="104"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="145"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8025936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="191" mass="22850" checksum="BD449D9481CB2BB8" modified="2023-09-13" version="1">MFYTNRRIKHCLADIQFNQFMHSCTDMSTLQIYKVFDMLDVDGSGKIDFDEFYLLACILISLKDKEEKQFIYRHSRTVFELLDEDGSQSISAGEFSAFGFLFNFHGDAVNQIFKDFDISGDQELDYKEFKMFAMACIDRQNDIDRRKREKAERRRRHQEEKMKRKEQKKLRQTEGLRRLSWDFLALSCVII</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1B9C2</accession>
    <name>A0A9Q1B9C2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ATP-dependent DNA helicase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_44503</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000939">
        <property type="protein sequence ID" value="KAJ8017840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1B9C2"/>
    <dbReference type="OrthoDB" id="6129494at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="entry name" value="DUF6570"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025476">
        <property type="entry name" value="Helitron_helicase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051055">
        <property type="entry name" value="PIF1_helicase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47642">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47642:SF5">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20209">
        <property type="entry name" value="DUF6570"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14214">
        <property type="entry name" value="Helitron_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6570" evidence="3">
        <location>
            <begin position="158"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="Helitron helicase-like" evidence="2">
        <location>
            <begin position="426"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="311"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="925"/>
            <end position="952"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1047"/>
            <end position="1089"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="311"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="321"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="928"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1062"/>
            <end position="1079"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF20209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8017840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1103" mass="128403" checksum="255D8068742C9F5A" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1B9T0</accession>
    <name>A0A9Q1B9T0_HOLLE</name>
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            <fullName evidence="12">Reverse transcriptase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_41675</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
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            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8019896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome 23"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Polyprotein of retroviral origin, putative"/>
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        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 297-like Protein"/>
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    </dbReference>
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        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon gypsy-like Protein"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="RT_dom"/>
    </dbReference>
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        <property type="entry name" value="RT_RNaseH"/>
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    </dbReference>
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        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
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        <location>
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            <end position="1021"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase" evidence="8">
        <location>
            <begin position="1277"/>
            <end position="1454"/>
        </location>
    </feature>
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        <location>
            <begin position="98"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
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            <end position="385"/>
        </location>
    </feature>
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        </location>
    </feature>
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            <begin position="696"/>
            <end position="736"/>
        </location>
    </feature>
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        <location>
            <begin position="329"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="344"/>
            <end position="369"/>
        </location>
    </feature>
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        <location>
            <begin position="371"/>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
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        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
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            <end position="736"/>
        </location>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8019896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1669" mass="190547" checksum="1D6A681AA1FFDC34" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BBD3</accession>
    <name>A0A9Q1BBD3_HOLLE</name>
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        <submittedName>
            <fullName evidence="18">Transmembrane protease serine 5</fullName>
        </submittedName>
    </protein>
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        <name evidence="18" type="ORF">HOLleu_40022</name>
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    <organism evidence="18 19">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
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        <lineage>
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            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
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            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Nanhai2018</strain>
            <tissue evidence="18">Muscle</tissue>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Putative serine protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SEA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003410">
        <property type="entry name" value="HYR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000082">
        <property type="entry name" value="SEA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036364">
        <property type="entry name" value="SEA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252">
        <property type="entry name" value="ACROSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252:SF7">
        <property type="entry name" value="HYALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02494">
        <property type="entry name" value="HYR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01390">
        <property type="entry name" value="SEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82671">
        <property type="entry name" value="SEA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50825">
        <property type="entry name" value="HYR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50024">
        <property type="entry name" value="SEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
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    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 12" id="KW-0720">Serine protease</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 14 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="51"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="SEA" evidence="15">
        <location>
            <begin position="113"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="HYR" evidence="17">
        <location>
            <begin position="260"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="HYR" evidence="17">
        <location>
            <begin position="420"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="domain" description="HYR" evidence="17">
        <location>
            <begin position="503"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="16">
        <location>
            <begin position="597"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="236"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50825"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAJ8020435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="942" mass="99677" checksum="CDEADDDC9FE344B7" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BC40</accession>
    <name>A0A9Q1BC40_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Nuclease HARBI1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_41449</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8019744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BC40"/>
    <dbReference type="OrthoDB" id="10051515at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027806">
        <property type="entry name" value="HARBI1_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13359">
        <property type="entry name" value="DDE_Tnp_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE Tnp4" evidence="3">
        <location>
            <begin position="2"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8019744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="140" mass="16061" checksum="64DB70908BB5259D" modified="2023-09-13" version="1">MKKPPNAGSYYYNYKGFHSIVLMAVVDATYKFLYVDVGAEGGASDVDKWSNCSLHDAVEENRAGVPQPEPLPNDDQPVPYHFVGDDAFALRTWMMKPFSLRSQVLRERIYSYRLSCARRVVENAFGILCHRFRCFLTTMH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BC84</accession>
    <name>A0A9Q1BC84_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Facilitated trehalose transporter Tret1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_39678</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BC84"/>
    <dbReference type="OrthoDB" id="6612291at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051119">
        <property type="term" value="F:sugar transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17358">
        <property type="entry name" value="MFS_GLUT6_8_Class3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Facilitated trehalose transporter Tret1-2 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044775">
        <property type="entry name" value="MFS_ERD6/Tret1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050549">
        <property type="entry name" value="MFS_Trehalose_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48021">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48021:SF1">
        <property type="entry name" value="GH07001P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="79"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="107"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="132"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="165"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="190"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="273"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="308"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="337"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="395"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="431"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="464"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="42"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8020163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="501" mass="54171" checksum="5D14C92723EFC714" modified="2023-09-13" version="1">MSSSKGDIQYGDQNVTEDHVALIPPDVGSRRVSSSSTFLYLVVAVSLLAPISMGYTIGYSSPALPDMKAAHVLQDETWFGVRSLLNIGAIFGGPLAGILLGKYGRKVTVMMCAVPYVIGWALIASIESQGGLFVGRVLTGLAAGMTSLSCPVYIAEMASPDNRGFLGSSFQLCVTLGILMVYSLGLPLSYVWLAIVAAGLAFLLVVFMLPMPDTPAYWLIKKQRQRALAVLQEVRGSGVDVDIECREIESTLDNSDNSISWREFLEPHLFRPLGISLSLMFFQQFSGINTVMFYTVDIFKSTHSSIDPHVSTIIVGAVQVAATAVSVLLMDRLGRKVLLIVAGVMMAISSTSFGAYYYIINEVNNGTNTTAAPIIPSSPSMTLAVADSSGADLSWLGLSSMVVYIIGFSLGLGPIPWLLMSEIFPSKARGIASAIATAFNWMCSFIITKEFLALEGAFTQQGVFWMYAGVCVVEVLFVLFLVPETKGKSLEEIEDHFRKRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BC88</accession>
    <name>A0A9Q1BC88_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tesmin/TSO1-like CXC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_41692</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8019909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BC88"/>
    <dbReference type="OrthoDB" id="8300196at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033467">
        <property type="entry name" value="Tesmin/TSO1-like_CXC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46704:SF9">
        <property type="entry name" value="BHLH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46704">
        <property type="entry name" value="CXC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01114">
        <property type="entry name" value="CXC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tesmin/TSO1-like CXC" evidence="1">
        <location>
            <begin position="113"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8019909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="171" mass="19352" checksum="82AA82E6E640BA92" modified="2023-09-13" version="1">MSQLGSTQNVSDSLMDACEKFVCKLYGSNVITSVNTLRFELFTRTRAKSRMLPPNKDSLTFHVKRANYQAFIWKRALEAKPKIDDPPGHGWKITGEMLEIEWMRLPPAPDAILELMSCNCKRDCSNNSCQCRKNNLHCTDACGCDKCVNQMEESDFSDDGGQESDEEEEAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BFG1</accession>
    <name>A0A9Q1BFG1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Tetraspanin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HOLleu_37649</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Nanhai2018</strain>
            <tissue evidence="16">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Part of TspanC8 subgroup, composed of 6 members that interact with the transmembrane metalloprotease ADAM10. This interaction is required for ADAM10 exit from the endoplasmic reticulum and for enzymatic maturation and trafficking to the cell surface as well as substrate specificity. Different TspanC8/ADAM10 complexes have distinct substrates. Promotes ADAM10-mediated cleavage of CDH2. Negatively regulates ligand-induced Notch activity probably by regulating ADAM10 activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Interacts with ADAM10; the interaction influences ADAM10 substrate specificity, endocytosis and turnover.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Late endosome membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Membrane</location>
            <topology evidence="14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the tetraspanin (TM4SF) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BFG1"/>
    <dbReference type="OrthoDB" id="2014092at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019899">
        <property type="term" value="F:enzyme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1450.10:FF:000011">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1450.10">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000301">
        <property type="entry name" value="Tetraspanin_animals"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018503">
        <property type="entry name" value="Tetraspanin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF431">
        <property type="entry name" value="TETRASPANIN 26A, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002419">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00259">
        <property type="entry name" value="TMFOUR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00421">
        <property type="entry name" value="TM4_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="48"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="93"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="125"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="261"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="9"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="186"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00056423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00065909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR002419-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU361218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ8022682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="296" mass="33055" checksum="786336187BB6F37B" modified="2023-09-13" version="1">MGKSKTTPTPYPYDDPPPYNDSHPPPPSRRPPVRSAPRGVEVSQCTKYLLFFINVFFWLIGAFLLGFGIWGLVSKNGGSVSALADDLGIILDPMFAFIISGAVIFILGFFGCIGSLRENTCMIKFFTYFLILIFLAEITIGVLAYVYQDQLFGLLESWFDKTIVLYLDDPDKQFIMDGLQESLRCCGSISPYDWETNPYYNCSSIARSACSVPFSCCLPEEGVINYQCGFDVLSLSDKAMKEVIYTYGCADAMRDWFITNAIILGCCVGALILFQCVTICLGRSLISDIQEVKSYW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BGM9</accession>
    <name>A0A9Q1BGM9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Fucosyltransferase</fullName>
            <ecNumber evidence="5">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_34384</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Golgi apparatus</location>
            <location evidence="5">Golgi stack membrane</location>
            <topology evidence="5">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the glycosyltransferase 10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="5"/>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BGM9"/>
    <dbReference type="OrthoDB" id="427096at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046920">
        <property type="term" value="F:alpha-(1-&gt;3)-fucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11660:FF:000004">
        <property type="entry name" value="Glycoprotein 3-alpha-L-fucosyltransferase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11660">
        <property type="entry name" value="Glycosyl transferase family 10, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055270">
        <property type="entry name" value="Glyco_tran_10_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001503">
        <property type="entry name" value="Glyco_trans_10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038577">
        <property type="entry name" value="GT10-like_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11929">
        <property type="entry name" value="ALPHA- 1,3 -FUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11929:SF145">
        <property type="entry name" value="ALPHA-(1,3)-FUCOSYLTRANSFERASE FUT-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00852">
        <property type="entry name" value="Glyco_transf_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <feature type="domain" description="Fucosyltransferase C-terminal" evidence="6">
        <location>
            <begin position="216"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8024469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="434" mass="51269" checksum="892F30BA09D575F7" modified="2023-09-13" version="1">MRLLEASENRVSRATIHRRAGSHTYQPVNLPKQKVRTVTQKQNINFNPNPGCASNPEILRGNGTRLTVAFYEGNRNVWSYPRTFNHPPSPRYCLCSSETRYFINVTSEKDPALLKTYDLVFMPQDVGTLRIKDQVWDTILNSTPSWQRRLYATWEGPSKIKKLLPSPTYRMKAYHWSLSFHSKSDFSVPYGYYRPYDKKSQSSRPEVVSGKNWAKGKFRLAAWMSTWKGTSWRRRDFVEGLTQFINIDLYGRRFQNCTRNSEVCEKRMKEYKFYFAIENSCCSEYITEKFWRTLAWDVVPVVIGAPKEDYERLAPPNSFIYADDFKSPHDLAKYLKQLDENDDLYNKFFQWKKVGRVYTNHPEIQQEFATSCIPPEHSYYSCNVICKVTDRYLEGKEDATLFNRKHRYFDPGSPWWTSSCTRCGTHMWIRKYNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BH02</accession>
    <name>A0A9Q1BH02_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transmembrane protein 183</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_37031</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8024317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BH02"/>
    <dbReference type="OrthoDB" id="5955317at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031647">
        <property type="term" value="P:regulation of protein stability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026509">
        <property type="entry name" value="TMEM183"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20988">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 183A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20988:SF2">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 183A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8024317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="388" mass="45058" checksum="8BA1AC3ED7D62B02" modified="2023-09-13" version="1">MYRRKKRRHRNWKKTASTKEASFLTDVTVQESADSPTPVVAIRKKNLSKSSLLQKHAMDGLSDSLNEETVPLDEVENWYDRDLSEVDNYLDDNLEAEVGREHVPKEKGKIQSKNCKKPEDKHIGRDYPADLWYLLADYIAPEDIGCFSALCKKALHATNSASFWRKLYNRYCKHDSASLPNRLQPYSIRNSDDCIRAKVICALYRVYPPFQERIKKGVRPLEDGDSHGLYGAVCQFVCNVGRVGNHYYHFIKFRHISSVTEQNLRNSLVSSPSVWRNPSEGEILLRVACPLFQPFSSEHIMGLQLASISLSVSRDMCYNRLRLEFERNYDKKKRRDPKRNGIDMYLDPVIHVSVMHWWHPLFHDSFAQPVSSQRTLRTQTPVDNHWDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BH36</accession>
    <name>A0A9Q1BH36_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="20">3-ketoacyl-CoA thiolase A, peroxisomal</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">HOLleu_34365</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">Nanhai2018</strain>
            <tissue evidence="20">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>2 acetyl-CoA = acetoacetyl-CoA + CoA</text>
            <dbReference type="Rhea" id="RHEA:21036"/>
            <dbReference type="ChEBI" id="CHEBI:57286"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.1.9"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="11">
            <dbReference type="Rhea" id="RHEA:21038"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>3-oxo-(6Z,9Z,12Z,15Z,18Z,21Z)-tetracosahexaenoyl-CoA + CoA = (4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoyl-CoA + acetyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:39131"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:74298"/>
            <dbReference type="ChEBI" id="CHEBI:74304"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:39132"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>3-oxohexadecanedioyl-CoA + CoA = tetradecanedioyl-CoA + acetyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:40343"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:77081"/>
            <dbReference type="ChEBI" id="CHEBI:77084"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:40344"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>an acyl-CoA + acetyl-CoA = a 3-oxoacyl-CoA + CoA</text>
            <dbReference type="Rhea" id="RHEA:21564"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="ChEBI" id="CHEBI:90726"/>
            <dbReference type="EC" id="2.3.1.16"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="14">
            <dbReference type="Rhea" id="RHEA:21566"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>hexanoyl-CoA + acetyl-CoA = 3-oxooctanoyl-CoA + CoA</text>
            <dbReference type="Rhea" id="RHEA:31203"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:62619"/>
            <dbReference type="ChEBI" id="CHEBI:62620"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="13">
            <dbReference type="Rhea" id="RHEA:31205"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>tetradecanoyl-CoA + acetyl-CoA = 3-oxohexadecanoyl-CoA + CoA</text>
            <dbReference type="Rhea" id="RHEA:18161"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57349"/>
            <dbReference type="ChEBI" id="CHEBI:57385"/>
            <dbReference type="EC" id="2.3.1.155"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="12">
            <dbReference type="Rhea" id="RHEA:18163"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 17">Belongs to the thiolase-like superfamily. Thiolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BH36"/>
    <dbReference type="OrthoDB" id="5404651at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003985">
        <property type="term" value="F:acetyl-CoA C-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050633">
        <property type="term" value="F:acetyl-CoA C-myristoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010124">
        <property type="term" value="P:phenylacetate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00751">
        <property type="entry name" value="thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000010">
        <property type="entry name" value="Acetyl-CoA acetyltransferase (Thiolase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050215">
        <property type="entry name" value="Thiolase-like_sf_Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020610">
        <property type="entry name" value="Thiolase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020617">
        <property type="entry name" value="Thiolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020613">
        <property type="entry name" value="Thiolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01930">
        <property type="entry name" value="AcCoA-C-Actrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43853">
        <property type="entry name" value="3-KETOACYL-COA THIOLASE, PEROXISOMAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43853:SF8">
        <property type="entry name" value="3-KETOACYL-COA THIOLASE, PEROXISOMAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02803">
        <property type="entry name" value="Thiolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00737">
        <property type="entry name" value="THIOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00099">
        <property type="entry name" value="THIOLASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 17" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Thiolase N-terminal" evidence="18">
        <location>
            <begin position="56"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase C-terminal" evidence="19">
        <location>
            <begin position="318"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="16">
        <location>
            <position position="141"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="16">
        <location>
            <position position="396"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="16">
        <location>
            <position position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00036770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR000429-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAJ8024452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="443" mass="46992" checksum="247F621DBB2D7866" modified="2023-09-13" version="1">MERFGTLVRQLARTSTVGQPGLQSTIKLHNDFLGNSRVLSMHPTRGTSSQTRDEDIVILSALRTPIGKGNKGSLKDTPIDVMLSSVLTAILDQTKLSPEVIGDIVVGNVSSIGAGAFQTRTAMFFSGFPYSVPVTSVNRQCASGLQAFITAAGQIKLGVYDIAIAGGMESMSMMKQDGMRKYVHEKIMANDEARDCLLPMGITSENVAQRYNIGREEQDKFALESQLKAARAQKEGWFDAEIVPVKTTVRDLSGQEQDVTISKDEGVRGNTTLAGLAKLKPAFLPDGCSTAGNSSQISDGAAAVLLSTQAKAQQLNLPVLGKLVSYAVVGVPPDIMGVGPAYAIPKALEKAGLTKDDIDIFEINEAFASQAVWCIKELGIPMEKVNPKGGAIALGHPLGCTGARMIGTLLHEMERRGKPGYGVVSMCMGSGMGAAAVFHYPGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BHL5</accession>
    <name>A0A9Q1BHL5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Solute carrier family 22 member 6-A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_31943</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BHL5"/>
    <dbReference type="OrthoDB" id="3936150at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24064">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="58"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8026960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="97" mass="10455" checksum="A665A93B18ED7F22" modified="2023-09-13" version="1">MIGKFGISASFALVYIYAVEVFPTPLRSVGLGMCSTASRIGSISTPLILLLDEIWEPLPLLIFGSSAIIGGLLVLFLPETRGKDLPETIEEGELFNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BIA5</accession>
    <name>A0A9Q1BIA5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SCAN box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_34121</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8026312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BIA5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF13">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCAN box" evidence="3">
        <location>
            <begin position="232"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="326"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="78"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="326"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="341"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="365"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8026312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="486" mass="55911" checksum="427D4E7DE55BF3E2" modified="2023-09-13" version="1">MPFNLDKFVASPSVEELDSLKKSEIVKVAKHYGVEFQPLMRKDEIKRYVLEYLVDESILPITVLETAITVPTDNTFELKRLEIEMNKEIRLKEMEREREREEREMQKEKEEREMQMQREKEAREHEFRLKQLELGVIKGSDPKIGLDTGGLFDVSKHVKFVPKFQEDNVEKFFNHFEKLGEQLKWPRDKWSILIQSNFTGKAQEVYSALSIEDSMDYDKVKKAILQAYELVPEDYRQKFRKYRKADSQTYVEFAYQKERHFDRWCASKKVSTFDTLRQLMLVEEFKRCVNDDIKTHLEENKADKLSEVANLADQYALTHKFSKVGQTQNKGQFSRPNKKYGNSSGTTGNSGSQSGSVSPTNSGKKQPDSSRQKNDQVSSNKAAFKDIIICGFCHKKGHTMANCFTLAKLKETETASNALANVENSSEVVVTLRPNSNDKIKEEYLPFVSEGFISLDGNSAHPPVKIKILRDTGATQSLLLDGVLPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BJP6</accession>
    <name>A0A9Q1BJP6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_29980</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8027895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BJP6"/>
    <dbReference type="OrthoDB" id="6361178at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8027895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="124" mass="13606" checksum="32208B7E0D3A458C" modified="2023-09-13" version="1">MPHIERKNPIVFGGGQRSPGVTGGQNLKTLLTQYLEMRYGKRKNPIIFGGGERSSGVTGGQTLKTSLTRYLKMRYGKRKNPIIFGGGERSSGVTGGQTLKTSLTRYLKKGSSDSSQTWYIDAPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BP25</accession>
    <name>A0A9Q1BP25_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_26740</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BP25"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8030348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="62" mass="7051" checksum="D314E8AC8EF19199" modified="2023-09-13" version="1">MKLTNLIDRLSTPLTADKCIRLNTGLFPNEVQDVLKLTNCTSMPSKRLQKRALLCPKFEGKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BPU0</accession>
    <name>A0A9Q1BPU0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_27358</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BPU0"/>
    <dbReference type="OrthoDB" id="120976at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006913">
        <property type="term" value="P:nucleocytoplasmic transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027038">
        <property type="entry name" value="RanGap"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24113">
        <property type="entry name" value="RAN GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24113:SF12">
        <property type="entry name" value="RAN GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="270"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="413"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="238"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="270"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="292"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="418"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8030828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="433" mass="48191" checksum="E589CC40B0A7013B" modified="2023-09-13" version="1">MPGKKKGGKKKKKGGKKKRPKGMLEDPGVVVKRLYKSYLENCAKKKSAVCPGLKAMMKDCYENDRLIVKFILEPVPDLNDPELKNVNIDPVISSIRQEWYRYIKEMHVWDIPVRHEEIANLALMLEKAVYPITRLDLVDCNMEAVSAARLVQKSSVLFLTSLCLDYNEFGDEGCLGLCKGLQGNKMLLSLSLCYCDLGIKSGTPLGELISSTAIRDVYLNGNNLEAEGAIELIKLSADQAEVESLQRAEEKQRKAEEEALALLAEKEKSRVRTAVSDDNKEDEKPSSARSSKSGKGKKKKKKGKKGKKKSKEPPPPPLVGPWIHKLHLADNGIDIYGAASNYGPVIAMRLFRQMLQHSSCFEELDLDDNLIGDLGGRDIMEGLKARKEEDLPGVKVSVTHRMNGETFDSIVKLGSGLQKKKKKKGKGKKKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BQT0</accession>
    <name>A0A9Q1BQT0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Trimethyllysine dioxygenase, mitochondrial</fullName>
            <ecNumber evidence="5">1.14.11.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Epsilon-trimethyllysine 2-oxoglutarate dioxygenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">TML hydroxylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">TML-alpha-ketoglutarate dioxygenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">HOLleu_27468</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">Nanhai2018</strain>
            <tissue evidence="19">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Converts trimethyllysine (TML) into hydroxytrimethyllysine (HTML).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>N(6),N(6),N(6)-trimethyl-L-lysine + 2-oxoglutarate + O2 = (3S)-3-hydroxy-N(6),N(6),N(6)-trimethyl-L-lysine + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:14181"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:58100"/>
            <dbReference type="ChEBI" id="CHEBI:141499"/>
            <dbReference type="EC" id="1.14.11.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amine and polyamine biosynthesis; carnitine biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the gamma-BBH/TMLD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.8" evidence="5"/>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BQT0"/>
    <dbReference type="OrthoDB" id="408743at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050353">
        <property type="term" value="F:trimethyllysine dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045329">
        <property type="term" value="P:carnitine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00250">
        <property type="entry name" value="CAS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2020.30:FF:000002">
        <property type="entry name" value="Putative gamma-butyrobetaine dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2020.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.130.10">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050411">
        <property type="entry name" value="AlphaKG_dependent_hydroxylases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010376">
        <property type="entry name" value="GBBH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038492">
        <property type="entry name" value="GBBH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042098">
        <property type="entry name" value="TauD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003819">
        <property type="entry name" value="TauD/TfdA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012776">
        <property type="entry name" value="Trimethyllysine_dOase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02410">
        <property type="entry name" value="carnitine_TMLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10696">
        <property type="entry name" value="GAMMA-BUTYROBETAINE HYDROXYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10696:SF51">
        <property type="entry name" value="TRIMETHYLLYSINE DIOXYGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06155">
        <property type="entry name" value="GBBH-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02668">
        <property type="entry name" value="TauD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0124">Carnitine biosynthesis</keyword>
    <keyword evidence="9 19" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gamma-butyrobetaine hydroxylase-like N-terminal" evidence="18">
        <location>
            <begin position="84"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="TauD/TfdA-like" evidence="17">
        <location>
            <begin position="181"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00016835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00046008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF06155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAJ8030920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="437" mass="49992" checksum="869D9ECB613965D5" modified="2023-09-13" version="1">MAYRLRLLSRRCQQLIITTRKWGSVSCNPNPYHVKWNTKGNSSLFCQHGLKVRWASHSTIGQCSDAWTMEVKDDHLLLNLEAVDLKFPYFWLRDNCRCSDCFHPQTHQVMHEFTDLDLHIRPADVNTVGDEVHITWNDNHKSSFTTQWLQKHAPGTRENMKTPSSILRVLWQGSTLGELPTFPLSELESEGGALRSLFRMLDQYGLALIEGVSTSTEAMESLAFTIAGGIESTFYSQGVTVMNANLEIADTGYTNIALPLHTDTTYFSTPCRAMMIHITKRDGQGGQTILSDGFFALERLRQSKPEYFDILCNTKLICQYVGENRNIRATGPVIKLNPDTNQVECLRFNPLDRAVISHLPYHQMLDFYKAYQALARELYKKEHSLTIQLNPGQVLLFDNWRVLHGRKSFTGNRVFKSAYLRKDDWMSQMRTVGGLNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BQW4</accession>
    <name>A0A9Q1BQW4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein FAM43A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_27807</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8031162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BQW4"/>
    <dbReference type="OrthoDB" id="5962185at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051133">
        <property type="entry name" value="Adapter_Engulfment-Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11232:SF2">
        <property type="entry name" value="FI05246P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11232">
        <property type="entry name" value="PHOSPHOTYROSINE INTERACTION DOMAIN-CONTAINING FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14719">
        <property type="entry name" value="PID_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00462">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PID" evidence="2">
        <location>
            <begin position="13"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="208"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="215"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8031162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="265" mass="30688" checksum="85980E91457CE0D8" modified="2023-09-13" version="1">MFRRKKKQFLVTPKDSEFIVQYLGSLRTLISKGEGCVEKPLEDIWRKSDHGMESVRTLLTVTKKGIMMKRLDPPRRLTAKEEPQEYYGEQLFMVNRISYCSTGKKLKAIFAWIYRHELRRSAVELRVHAVVCQSAEVAYALGSLVWEKFQFCFRDFKLDRKASDRVLERQQETEDSKQRALPMEVPLRTKMNSRPAYKVEKMPVGKTKSLAAIKKSNENTESAQERKSHEAEDVDDFENGLGFVESAIHTENADFIKANTASNLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-04-02" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BTQ1</accession>
    <name>A0A9Q1BTQ1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Endonuclease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_25921</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="115435at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000032">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 297-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon gypsy-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
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    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF15">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="3">
        <location>
            <begin position="832"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="1248"/>
            <end position="1371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="298"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="566"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="665"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="298"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="313"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="340"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="566"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="589"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="609"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="634"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="681"/>
            <end position="698"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8032404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1371" mass="157137" checksum="7AD8E1F40CED97A5" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BUJ5</accession>
    <name>A0A9Q1BUJ5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">General transcription factor 3C polypeptide 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_22904</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8032841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BUJ5"/>
    <dbReference type="OrthoDB" id="5598268at2759"/>
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        <property type="component" value="Chromosome 11"/>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:RNA polymerase III type 1 promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001003">
        <property type="term" value="F:RNA polymerase III type 2 promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006384">
        <property type="term" value="P:transcription initiation at RNA polymerase III promoter"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Transcription factor IIIC, subunit 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.160">
        <property type="entry name" value="TFIIIC, subcomplex tauA, subunit Sfc1, barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TF_IIIC_su-5_HTH"/>
    </dbReference>
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        <property type="entry name" value="TF_IIIC_Tfc1/Sfc1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041499">
        <property type="entry name" value="Tfc1/Sfc1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042536">
        <property type="entry name" value="TFIIIC_tauA_Sfc1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13230:SF5">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR 3C POLYPEPTIDE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13230">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIIC, POLYPEPTIDE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Tau95"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Tau95_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="Transcription factor IIIC subunit Tfc1/Sfc1 triple barrel" evidence="7">
        <location>
            <begin position="32"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription factor IIIC subunit 5 HTH" evidence="6">
        <location>
            <begin position="185"/>
            <end position="330"/>
        </location>
    </feature>
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        <location>
            <begin position="103"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="362"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="457"/>
            <end position="526"/>
        </location>
    </feature>
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        <location>
            <begin position="108"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="471"/>
            <end position="513"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF09734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF17682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8032841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="526" mass="60142" checksum="FACE79419AB11D52" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BW72</accession>
    <name>A0A9Q1BW72_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_24407</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8034001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BW72"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8034001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="100" mass="11598" checksum="82D13D005D2DE9EE" modified="2023-09-13" version="1">MWKCRHIHSSGLVCFKVWYPCRRPASTQVGEGPCNSRQVKCKVNHMAQPVNLDGEGLSAEHGKHKESVTQNCKEDFLRKNVRFGRTIQLKRGLSYTRHYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BX79</accession>
    <name>A0A9Q1BX79_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_21368</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BX79"/>
    <dbReference type="OrthoDB" id="10505967at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8034502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="125" mass="14524" checksum="BB2E6665472384F4" modified="2023-09-13" version="1">MTFLRKVECLSVSFFKTCLFLSFFPLFSFAFVGEFAEFLVSVAVRLTNTNCVYLANYFNFPPPVIELVRRDLETPGITLINIMRERNVINMHDVTGLQRALSGLELNDINEVLVIPYQERIDFFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BXM5</accession>
    <name>A0A9Q1BXM5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_21419</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BXM5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8034538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="158" mass="18034" checksum="69FC41CFC4E75D96" modified="2023-09-13" version="1">MPSTKNTYSLLGEEPHWEKQSPSTCELTVKACLITRNEFCWVKCIIDGGVVEAKHLQVYTACKRISTVDKITKIEVGYYPDLDGAGKLLRLNRNWIVTEKRPFLFLKEGGAPLQILFIEIAPPVWKYCLPNDNPKVMKSIKLYFSSSVFRRHLGLSLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BY92</accession>
    <name>A0A9Q1BY92_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Nodal modulator 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HOLleu_22077</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Nanhai2018</strain>
            <tissue evidence="18">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the multi-pass translocon (MPT) complex that mediates insertion of multi-pass membrane proteins into the lipid bilayer of membranes. The MPT complex takes over after the SEC61 complex: following membrane insertion of the first few transmembrane segments of proteins by the SEC61 complex, the MPT complex occludes the lateral gate of the SEC61 complex to promote insertion of subsequent transmembrane regions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BY92"/>
    <dbReference type="OrthoDB" id="10263633at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160064">
        <property type="term" value="C:multi-pass translocon complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160063">
        <property type="term" value="P:multi-pass transmembrane protein insertion into ER membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1120:FF:000001">
        <property type="entry name" value="Nodal modulator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1120">
        <property type="entry name" value="Carboxypeptidase-like, regulatory domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013784">
        <property type="entry name" value="Carb-bd-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008969">
        <property type="entry name" value="CarboxyPept-like_regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055075">
        <property type="entry name" value="NOMO-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055074">
        <property type="entry name" value="NOMO1-3_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055073">
        <property type="entry name" value="NOMO1-like_9th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056191">
        <property type="entry name" value="NOMO_12th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056190">
        <property type="entry name" value="NOMO_5th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056319">
        <property type="entry name" value="NOMO_7th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056187">
        <property type="entry name" value="NOMO_8th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051417">
        <property type="entry name" value="SDr/BOS_complex"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23303:SF14">
        <property type="entry name" value="BOS COMPLEX SUBUNIT NOMO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23303">
        <property type="entry name" value="CARBOXYPEPTIDASE REGULATORY REGION-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13620">
        <property type="entry name" value="CarboxypepD_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23141">
        <property type="entry name" value="Ig_NOMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22898">
        <property type="entry name" value="NOMO1-like_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22904">
        <property type="entry name" value="NOMO1-like_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22902">
        <property type="entry name" value="NOMO1-like_9th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23192">
        <property type="entry name" value="NOMO_12th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23194">
        <property type="entry name" value="NOMO_5th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23660">
        <property type="entry name" value="NOMO_8th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49464">
        <property type="entry name" value="Carboxypeptidase regulatory domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49478">
        <property type="entry name" value="Cna protein B-type domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49452">
        <property type="entry name" value="Starch-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040498059" evidence="10">
        <location>
            <begin position="22"/>
            <end position="1206"/>
        </location>
    </feature>
    <feature type="domain" description="NOMO-like N-terminal beta-sandwich" evidence="11">
        <location>
            <begin position="28"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="NOMO second beta-sandwich" evidence="13">
        <location>
            <begin position="114"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="NOMO fifth transthyretin-like" evidence="16">
        <location>
            <begin position="396"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="domain" description="NOMO seventh transthyretin-like" evidence="14">
        <location>
            <begin position="579"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="domain" description="NOMO eighth prealbumin-like" evidence="17">
        <location>
            <begin position="688"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="domain" description="NOMO-like ninth beta-sandwich" evidence="12">
        <location>
            <begin position="766"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="domain" description="NOMO C-terminal transthyretin-like" evidence="15">
        <location>
            <begin position="1026"/>
            <end position="1119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="677"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1171"/>
            <end position="1206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="677"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="691"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="1175"/>
            <end position="1184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="1197"/>
            <end position="1206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00056484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF22898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF22902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF22904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF23141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF23192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF23194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF23660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ8035005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1206" mass="131921" checksum="96F68AAD83D4D1C2" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BYR5</accession>
    <name>A0A9Q1BYR5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Speckle-type POZ protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_19696</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Tdpoz family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8035883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BYR5"/>
    <dbReference type="OrthoDB" id="6359816at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18345">
        <property type="entry name" value="BTB_POZ_roadkill-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.210.10:FF:000028">
        <property type="entry name" value="Speckle-type POZ protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000008">
        <property type="entry name" value="Speckle-type POZ protein-like a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.250.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.210.10">
        <property type="entry name" value="Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056423">
        <property type="entry name" value="BACK_BPM_SPOP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002083">
        <property type="entry name" value="MATH/TRAF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008974">
        <property type="entry name" value="TRAF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24413">
        <property type="entry name" value="SPECKLE-TYPE POZ PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24570">
        <property type="entry name" value="BACK_BPM_SPOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22486">
        <property type="entry name" value="MATH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00061">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50144">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="MATH" evidence="7">
        <location>
            <begin position="18"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="6">
        <location>
            <begin position="187"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8035883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="362" mass="41032" checksum="3EA429CDB8578656" modified="2023-09-13" version="1">MSCPVAENWCYTQVKVVKFSYMWTINNFSFCREEMGEVLKSSTFSSGANDKLKWCLRVNPKGLDEESKDYLSLYLLLVSCNKSEVRAKFKFSILNAKREETKAMESQRAYKFVQGKDWGFKKFIRRDFLMDEANGLLPDDKLTLFCEVSIVADTVNISGQSTQAQLKVPDCKLADDLSSLWERSAFSDVTLCVNGREFQAHKAILAARSPVFNAMFEHEMEEKKQNRVEITDVDQDVMKEILRFIYTGTAPNLEKMADDLLAAADKYALERLKVMCEEALCSNLSTDNVADVLVLADLHSADQLKTVAIDYVNSHAPEVMDTNGWKNMVCNHANLVADAFRALVTSQSPPFMGPPRKRLKQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C3P5</accession>
    <name>A0A9Q1C3P5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sulfurtransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_18760</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C3P5"/>
    <dbReference type="OrthoDB" id="270167at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004792">
        <property type="term" value="F:thiosulfate-cyanide sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01448">
        <property type="entry name" value="TST_Repeat_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01449">
        <property type="entry name" value="TST_Repeat_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.250.10:FF:000001">
        <property type="entry name" value="Sulfurtransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.250.10:FF:000015">
        <property type="entry name" value="Sulfurtransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001307">
        <property type="entry name" value="Thiosulphate_STrfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045078">
        <property type="entry name" value="TST/MPST-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11364:SF27">
        <property type="entry name" value="SULFURTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11364">
        <property type="entry name" value="THIOSULFATE SULFERTANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00380">
        <property type="entry name" value="RHODANESE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00683">
        <property type="entry name" value="RHODANESE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Rhodanese" evidence="6">
        <location>
            <begin position="20"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodanese" evidence="6">
        <location>
            <begin position="170"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8037840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="296" mass="32968" checksum="7B73C8F7F61A6703" modified="2023-09-13" version="1">MHRVSPLVTTGWLSKVLTPGSQGIRVLDGSWYMPTSEGDAHKDYLEKHIPGAAFFDSEKVSDLTSPYSHTVPHPRLFGDYVGKLGITNDTHVIVYDNHKEYGMFSSPRVWWIMRLFGHHRVSVLEGGLPKWISEGHPTTHEVPQIEKTEYNVNFQPQLLKKFEDMMQNLKTKKFQVMDARSRARFAGTVEEPRKGSLKLGHIPNSKNIPFMDLLDPETQQMKPPEELKKIFAEGGIDLTKPLTATCGSGITACIISLGAFLSGKEDVSVFDGSWEEYAQRASSEDIVSAPQGPAGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C4E9</accession>
    <name>A0A9Q1C4E9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">alpha-1,2-Mannosidase</fullName>
            <ecNumber evidence="7">3.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_15923</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the glycosyl hydrolase 47 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.-" evidence="7"/>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C4E9"/>
    <dbReference type="OrthoDB" id="8118055at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044322">
        <property type="term" value="C:endoplasmic reticulum quality control compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004571">
        <property type="term" value="F:mannosyl-oligosaccharide 1,2-alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904380">
        <property type="term" value="P:endoplasmic reticulum mannose trimming"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044674">
        <property type="entry name" value="EDEM1/2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001382">
        <property type="entry name" value="Glyco_hydro_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036026">
        <property type="entry name" value="Seven-hairpin_glycosidases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45679">
        <property type="entry name" value="ER DEGRADATION-ENHANCING ALPHA-MANNOSIDASE-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45679:SF2">
        <property type="entry name" value="ER DEGRADATION-ENHANCING ALPHA-MANNOSIDASE-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01532">
        <property type="entry name" value="Glyco_hydro_47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00747">
        <property type="entry name" value="GLYHDRLASE47"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48225">
        <property type="entry name" value="Seven-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040162032" description="alpha-1,2-Mannosidase" evidence="9">
        <location>
            <begin position="26"/>
            <end position="1123"/>
        </location>
    </feature>
    <feature type="domain" description="PA" evidence="10">
        <location>
            <begin position="666"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="490"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="532"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="844"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="936"/>
            <end position="960"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="995"/>
            <end position="1123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="490"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="538"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="936"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1020"/>
            <end position="1062"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1076"/>
            <end position="1102"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="128"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="271"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="365"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="383"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="469"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8038486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1123" mass="124825" checksum="439DD6FB3943CB03" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C4L8</accession>
    <name>A0A9Q1C4L8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Carboxypeptidase N subunit 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_18732</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C4L8"/>
    <dbReference type="OrthoDB" id="694479at2759"/>
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        <property type="component" value="Chromosome 8"/>
    </dbReference>
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        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050328">
        <property type="entry name" value="Dev_Immune_Receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000372">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR24373:SF370">
        <property type="entry name" value="FISH-LIPS, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24373">
        <property type="entry name" value="SLIT RELATED LEUCINE-RICH REPEAT NEURONAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00013">
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="421"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="domain" description="LRRNT" evidence="5">
        <location>
            <begin position="11"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8037821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="579" mass="64934" checksum="9C134189F4780DB9" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C5G0</accession>
    <name>A0A9Q1C5G0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_16611</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C5G0"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8039026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="127" mass="14093" checksum="1FF64262690D82F5" modified="2023-09-13" version="1">MTGPSQSSSIFREHFTDDYDPSLALKEKMGLAAGKLKRTPLSGKFPTVKVPSKSSGMQTKRSGGAEESRPNIRLSTSKEVTPSTSGALWQPVWKKPRLSLEKRQRKEVMSCLGWNCIIVTLECQQSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C5P3</accession>
    <name>A0A9Q1C5P3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Tyramine receptor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HOLleu_13856</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Nanhai2018</strain>
            <tissue evidence="18">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8039753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C5P3"/>
    <dbReference type="OrthoDB" id="5977853at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060170">
        <property type="term" value="C:ciliary membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00637">
        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22752">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22752:SF10">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0969">Cilium</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 18" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16" id="KW-0732">Signal</keyword>
    <keyword evidence="13" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040128677" evidence="16">
        <location>
            <begin position="20"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="51"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="192"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="232"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="17">
        <location>
            <begin position="1"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004309"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ8039753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="275" mass="30968" checksum="8A65E3DD728EC33B" modified="2023-09-13" version="1">MKLHHVFLMIAGGWFCALAMVIPPLTGSASTSVYTHGTHHCSPSWKDSCLYYNIGVALVYCITIPTLVICYGLITLTIKRSADRLADYAKRGQRDVQFTVLPTTDNTQNDPRIEKNQVEASSHQEDTFQEDSFVLNSCSNAQSFDQISSISDRLDVNSQLKQLPVTEKGDGIVKEKPAHSKLPHYRRYDKKVALSGAMLVLTTTVCWTPYFIVHTCNVEKTPSHGLEVFTMWLAYFNAALDPVIYTMLNKKIHKAVMEPFHFLRASITSKCFHSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C685</accession>
    <name>A0A9Q1C685_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_16147</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DoxX family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C685"/>
    <dbReference type="OrthoDB" id="432685at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040399">
        <property type="entry name" value="TMEM35A/B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13163">
        <property type="entry name" value="SPINAL CORD EXPRESSION PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13163:SF2">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 35B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="81"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="105"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8038661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="139" mass="15142" checksum="AB439315F0CA6E9D" modified="2023-09-13" version="1">MGLANYFAIIIGLLFVYFGTLKLVPINLSETEGYDQMHKDFEKFATVLPWSVDPSFYCCLFGWIEVIGGSLMAFGRQPYRTLGALDILLLMLDGIYTVVMLGESVLTIGGVIFGAACALAVIVFRDALDGDTGGKPKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C6D9</accession>
    <name>A0A9Q1C6D9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ras GTPase-activating protein nGAP</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_17287</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C6D9"/>
    <dbReference type="OrthoDB" id="6158299at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021887">
        <property type="entry name" value="DAB2P_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12004">
        <property type="entry name" value="DAB2P_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Disabled homolog 2-interacting protein C-terminal" evidence="3">
        <location>
            <begin position="10"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="96"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="14"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8039536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="304" mass="35525" checksum="DD93DD9D5004BE66" modified="2023-09-13" version="1">MAEGESPCNRPKNSKQEQEEIQELRERLERAERRNAELSEQLKMHKLHTKQLLLGWELKVKTSNDIITSQARERDHQLTEIASDLLYFESCLLKHRTKAKEKLTEKNRKISELQRKSKHQEKQINALNNANERLLASLHEIRESYEDRKLDTVSGYDSEETLCDNRDHMDRVQFVNGSTYNGDISGRERERESSSNVSLEILSAKQTGNPRFRNGRSRTRKFSDVNVCSHLGNPYEMKTNGIALPRSQQQISHKVRFTPDVKEISEEEDRDKPNDIVKPVLKIRKISLPAYRLPITDDEKEEYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C9S6</accession>
    <name>A0A9Q1C9S6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Reverse transcriptase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_15463</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10025340at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00303">
        <property type="entry name" value="retropepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09274">
        <property type="entry name" value="RNase_HI_RT_Ty3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.370:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 17.6-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000032">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 297-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon gypsy-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000020">
        <property type="entry name" value="Transposon Tf2-6 polyprotein-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041373">
        <property type="entry name" value="RT_RNaseH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17917">
        <property type="entry name" value="RT_RNaseH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50804">
        <property type="entry name" value="SCAN_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SCAN box" evidence="9">
        <location>
            <begin position="162"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase catalytic" evidence="11">
        <location>
            <begin position="676"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase" evidence="10">
        <location>
            <begin position="1092"/>
            <end position="1269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="249"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="45"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="253"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="278"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8040987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1485" mass="169080" checksum="2EEF3CC3DF5628E6" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CAW6</accession>
    <name>A0A9Q1CAW6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Tenascin-R</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_12108</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CAW6"/>
    <dbReference type="OrthoDB" id="7250310at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00087">
        <property type="entry name" value="FReD"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="TIL"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050373">
        <property type="entry name" value="Fibrinogen_C-term_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024731">
        <property type="entry name" value="NELL2-like_EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036084">
        <property type="entry name" value="Ser_inhib-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002919">
        <property type="entry name" value="TIL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025615">
        <property type="entry name" value="TILa_dom"/>
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    <dbReference type="NCBIfam" id="NF040941">
        <property type="entry name" value="GGGWT_bact"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143:SF458">
        <property type="entry name" value="FIBRINOGEN C-TERMINAL DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143">
        <property type="entry name" value="FIBRINOGEN/TENASCIN/ANGIOPOEITIN"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF12947">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01826">
        <property type="entry name" value="TIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12714">
        <property type="entry name" value="TILa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00186">
        <property type="entry name" value="FBG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57567">
        <property type="entry name" value="Serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="2"/>
    </dbReference>
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    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1 3" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="23"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="6">
        <location>
            <begin position="46"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="257"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="377"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="6">
        <location>
            <begin position="414"/>
            <end position="653"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8041319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="653" mass="74376" checksum="DB1DBA7A802519B7" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CB72</accession>
    <name>A0A9Q1CB72_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Raftlin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_12448</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="5970923at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="485"/>
            <end position="982"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1008"/>
            <end position="1077"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1091"/>
            <end position="1258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1277"/>
            <end position="1313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="518"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="555"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="591"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="626"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="703"/>
            <end position="717"/>
        </location>
    </feature>
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        <location>
            <begin position="726"/>
            <end position="739"/>
        </location>
    </feature>
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        <location>
            <begin position="762"/>
            <end position="771"/>
        </location>
    </feature>
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        <location>
            <begin position="810"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
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        </location>
    </feature>
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        <location>
            <begin position="887"/>
            <end position="896"/>
        </location>
    </feature>
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        <location>
            <begin position="915"/>
            <end position="926"/>
        </location>
    </feature>
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        <location>
            <begin position="927"/>
            <end position="936"/>
        </location>
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        <location>
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            <end position="974"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1028"/>
            <end position="1049"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1197"/>
            <end position="1211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1218"/>
            <end position="1229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8041586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1399" mass="157426" checksum="9DE56338C72C641C" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CBQ9</accession>
    <name>A0A9Q1CBQ9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Methyltransferase-like protein 17, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_12948</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Mitochondrial ribosome (mitoribosome) assembly factor. Binds at the interface of the head and body domains of the mitochondrial small ribosomal subunit (mt-SSU), occluding the mRNA channel and preventing compaction of the head domain towards the body. Probable inactive methyltransferase: retains the characteristic folding and ability to bind S-adenosyl-L-methionine, but it probably lost its methyltransferase activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CBQ9"/>
    <dbReference type="OrthoDB" id="421327at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052571">
        <property type="entry name" value="Mt_RNA_Methyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015324">
        <property type="entry name" value="Ribosomal_Rsm22-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13184">
        <property type="entry name" value="37S RIBOSOMAL PROTEIN S22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13184:SF5">
        <property type="entry name" value="METHYLTRANSFERASE-LIKE PROTEIN 17, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09243">
        <property type="entry name" value="Rsm22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="454"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="469"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00045681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8041992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="492" mass="57362" checksum="B425EF48ACAE3592" modified="2023-09-13" version="1">MSRLWKRERVSQFMKFAQRLAQLRCGHVVELDPSVAATLESSSPRRHPGIIQPKIIRLPESLQKNISNVYREQRIPNLQLQASALANYLKSRKRPVENSDVREAARYYTEKLGYTDTSKLDPEEEKKIATKVKNKLRKHLYHWKPISYDEKMCLVYLAARMAPNYAAIFRVLYEIKRRLPGFQPSSVLDFGSGLGSSVWAVNSLFQESVKEYYCVDVSQAMHNLAERLLWKPQHKGENADDLIIPGIYHRFFLPVSPKTTFDITISNNSLLEIQSHEERMKLLDILWNKTKDFLILIENGSLEGYYTLMEARNMILAKGQNEVSFNPEWCKGEEGYVFAPCSHDVICPRLVKDNFRPCNFEQKYEPLHIPGTKSQVKETERFSYLVLRKGSRPEGTVWPRVISEVLLRARHRVCRLCHTDGNLREVIVTKKKHEKNIYRCVRYTQWGDMLPVSRIESSSSEERKVREQSDDEMKDDSNEDGDTDVELRDEER</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CDT7</accession>
    <name>A0A9Q1CDT7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Glutathione S-transferase theta-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_10192</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the GST superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CDT7"/>
    <dbReference type="OrthoDB" id="422574at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051369">
        <property type="entry name" value="GST_Theta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43917">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43917:SF8">
        <property type="entry name" value="GH16740P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01153">
        <property type="entry name" value="Main.4:_Theta-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="4">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="5">
        <location>
            <begin position="90"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8043207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="242" mass="28366" checksum="12A74423A6317432" modified="2023-09-13" version="1">MALQFYYYPLSMPSRSVRLFLKKTGIHVEEHIIDLSKEEQHSPEFLKINPRHCVPVLIDGGFVLTEGWAILAYLADRFPDKVADHWYPKELQKRARVNEYLSYHDGSTRQLFSKVFQDEFLCKMRDWVKPPSKDEAEQHVKDMQYVATQLQDTFLKDKPFLCGEEISIADILALAEVIQPTISGRDTTKDHSKLAEWVERVKERLNPEFDEVYKHFYDVRKEFMSASNPSNAIERKVIQPKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CEK7</accession>
    <name>A0A9Q1CEK7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ubiquitin carboxyl-terminal hydrolase 32</fullName>
            <ecNumber evidence="2">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HOLleu_11206</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Nanhai2018</strain>
            <tissue evidence="16">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="2"/>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="265776at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000081">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000018">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2230.10">
        <property type="entry name" value="DUSP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035927">
        <property type="entry name" value="DUSP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006615">
        <property type="entry name" value="Pept_C19_DUSP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028135">
        <property type="entry name" value="Ub_USP-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057368">
        <property type="entry name" value="USP32_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF76">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06337">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14836">
        <property type="entry name" value="Ubiquitin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25265">
        <property type="entry name" value="USP32_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00695">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143791">
        <property type="entry name" value="DUSP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51283">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="8 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="EF-hand" evidence="13">
        <location>
            <begin position="232"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="13">
        <location>
            <begin position="268"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="13">
        <location>
            <begin position="322"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="domain" description="DUSP" evidence="15">
        <location>
            <begin position="377"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="14">
        <location>
            <begin position="748"/>
            <end position="1620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="428"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1017"/>
            <end position="1138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1205"/>
            <end position="1235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1404"/>
            <end position="1427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1442"/>
            <end position="1464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1532"/>
            <end position="1555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="431"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="443"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1027"/>
            <end position="1037"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1069"/>
            <end position="1094"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1117"/>
            <end position="1137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1442"/>
            <end position="1455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00071642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ8043901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1656" mass="184917" checksum="E01CE8BA74A6BAE3" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CFR3</accession>
    <name>A0A9Q1CFR3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Required for meiotic nuclear division protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_11057</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RMD1/sif2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CFR3"/>
    <dbReference type="OrthoDB" id="242766at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070131">
        <property type="term" value="P:positive regulation of mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003734">
        <property type="entry name" value="DUF155"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051624">
        <property type="entry name" value="RMD1/Sad1-interacting"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16255">
        <property type="entry name" value="REQUIRED FOR MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16255:SF1">
        <property type="entry name" value="REQUIRED FOR MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02582">
        <property type="entry name" value="DUF155"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF155" evidence="3">
        <location>
            <begin position="206"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="113"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="115"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8043794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="429" mass="49547" checksum="489C3839530BCE8D" modified="2023-09-13" version="1">MVIRESWTFGRYVTNISMTCFEPFQQTAFACKFLKNSFPFHGRIQLRNFHNAPGTFLLNKCCSYHIRSRVLSSSAWDNTKVSLQESISRHLHEKKAPSQGLCSDILRKMSTSVYSPTKEPSSSTKPLKSTKVARPRTRKPAAFVPKDEEKWLSTAFSLAEEFHLEHLSLDLQTQGNIQVASLPTDAHDVLHMNVITAEETAEPSEMFIFRNGCLVFWNVPEPMIKKVMKTAAKHQSEPYEIALVNWESEQMNYTYEDGNTRLVKDSIVLNASSPPDVTTLEKFTFSNAMMLSVKLAMWEYMLDKFVSSIEYIPETLKAHGKLKMSHKNTMKHMGKLYSLSHRINLSSDLLNTPDFYWDREELEKIFNKMCRFLSVARRTEVMNEKLKHCHELMDLMRNDVTEQHTLRLEAMITILIGVEILIEISHYTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CGJ0</accession>
    <name>A0A9Q1CGJ0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_07790</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CGJ0"/>
    <dbReference type="OrthoDB" id="1917519at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8044902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="64" mass="7524" checksum="FCA98170C52CF45C" modified="2023-09-13" version="1">MLRGRTLLFLVEVKGHLRSPEVKNRKLCKHDISRFVMLRGRTLSFLVEVKGHLRSPEVKLRKTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CGM0</accession>
    <name>A0A9Q1CGM0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Univin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_07864</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the TGF-beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CGM0"/>
    <dbReference type="OrthoDB" id="5987191at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.90.10:FF:000001">
        <property type="entry name" value="Bone morphogenetic protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001839">
        <property type="entry name" value="TGF-b_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001111">
        <property type="entry name" value="TGF-b_propeptide"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015615">
        <property type="entry name" value="TGF-beta-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017948">
        <property type="entry name" value="TGFb_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848:SF300">
        <property type="entry name" value="CVG1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848">
        <property type="entry name" value="TGF-BETA FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00019">
        <property type="entry name" value="TGF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00688">
        <property type="entry name" value="TGFb_propeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00204">
        <property type="entry name" value="TGFB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00250">
        <property type="entry name" value="TGF_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51362">
        <property type="entry name" value="TGF_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0339">Growth factor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040342717" evidence="9">
        <location>
            <begin position="18"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="domain" description="TGF-beta family profile" evidence="10">
        <location>
            <begin position="293"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8044958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="413" mass="47043" checksum="D9397A41409AEF78" modified="2023-09-13" version="1">MGFRLIAIAFLTALVSSTVSLSRSQSFSQNGDRSVEKGTSEKMETQMLKILGLRRRPRPKGNATAPQFMWDMYNLMTQYKDTNEREEEDGSRDCVYLDPAIPGNIVRSFPCKASKWWRFALYLTYDVTSASSARLSSVQRPRSRVKASYQTDWVLSFNLSSIKSTEKLTKADLRLASLINNRISNFAEKFQLAITDLSTSKRAKAFKEDVRSLKDILGNHTGPEEELSVDVLSLVQHWRVNDVTSHGLVVSLRCMVKETECERFMTKLRRSAPQQQSVNLVTVSMEGERCKERSRRSVNDGDQPVKMSSNLCQRHSLYVGFRDVGWDDWIIAPTGYQAYYCAGECPFPIGEQQNGTNHAVIQTLVRRFHYRSVPDVCCAPVRLSPISLLFFDDNDNVVLRRYDDMIVESCGCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CGY0</accession>
    <name>A0A9Q1CGY0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_07611</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CGY0"/>
    <dbReference type="OrthoDB" id="5982503at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044782">
        <property type="term" value="P:cilium organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028039">
        <property type="entry name" value="CCDC32"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31800">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31800:SF1">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14989">
        <property type="entry name" value="CCDC32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8044766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="170" mass="19303" checksum="E0C9CF138E0670A5" modified="2023-09-13" version="1">MADPNTKELDPFKVDNSPSGELSDNDRCFQDNFAPLAPIPGPVLTNDEAHQSLQSRVYLAHLENKLKKLKGQNPSSRVSSKDIITSLCKVRLDASENLVSTENTVFSEEPVGEQRLVEVNDLKRRMLPEQALTLEELTYLLERDYLNAVTQYIRKDDDDNEDENIDNQAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CHE0</accession>
    <name>A0A9Q1CHE0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_07892</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CHE0"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8044980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="77" mass="8543" checksum="E21C2C4685A04F60" modified="2023-09-13" version="1">MSSQFPCTDQAYTAASGFVLGSFPTAKGCQLQTPEFSYPNTYKMNKVCMLEEQARSPLPPISEKNYRTSLNVSRPCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CI23</accession>
    <name>A0A9Q1CI23_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HTH luxR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_08880</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CI23"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="C-terminal effector domain of the bipartite response regulators"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH luxR-type" evidence="1">
        <location>
            <begin position="19"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8045797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="68" mass="7849" checksum="A1CA8EA85DA67B64" modified="2023-09-13" version="1">MAEYSPRLEREEGRLIEDVEMGFSNKEIAELLKISNSTVKRRRAELGVRKKGSNYSHECSEVNEADVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CJU9</accession>
    <name>A0A9Q1CJU9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Calmodulin-lysine N-methyltransferase</fullName>
            <ecNumber evidence="3">2.1.1.60</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_09248</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.60" evidence="3"/>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8046073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CJU9"/>
    <dbReference type="OrthoDB" id="413520at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018025">
        <property type="term" value="F:calmodulin-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025800">
        <property type="entry name" value="CaM-Lys-N-MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019410">
        <property type="entry name" value="Methyltransf_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13539">
        <property type="entry name" value="CALMODULIN-LYSINE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13539:SF3">
        <property type="entry name" value="CALMODULIN-LYSINE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10294">
        <property type="entry name" value="Methyltransf_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8046073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="303" mass="34573" checksum="2B1BB055B038420C" modified="2023-09-13" version="1">MQKPADKTISKQRTLAKSRWKLIYHALKKDSTNADISSDLHQNVSVLRFKSFGFVLQKDIEDSKDCENASWSHCTCSLVPEFSLKIRQLNLRPTAKDLIGFNNTGNVCIWPAEEVLTYYCLKNRSFLRSKSVCELGGGMTCLAGLAVACSSDANEVVVTDGNEQSCQNAETIRKENTSKFGCTQVSVKQVLWNKEETFADLREKFDMVLGADCFFFDEYRQDLVNTITAILKPGGTAWMFAPHRGSTLRQFESVAQKNFSTNLVENYDHLVWERHQNMKSTREVTGQYDEDIHYPWMLTLCKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CKG6</accession>
    <name>A0A9Q1CKG6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Metalloproteinase inhibitor 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_05058</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protease inhibitor I35 (TIMP) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CKG6"/>
    <dbReference type="OrthoDB" id="6041373at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008191">
        <property type="term" value="F:metalloendopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002020">
        <property type="term" value="F:protease binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051045">
        <property type="term" value="P:negative regulation of membrane protein ectodomain proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.370.10">
        <property type="entry name" value="Tissue inhibitor of metalloproteinase-1. Chain B, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001134">
        <property type="entry name" value="Netrin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001820">
        <property type="entry name" value="TIMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008993">
        <property type="entry name" value="TIMP-like_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027465">
        <property type="entry name" value="TIMP_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11844">
        <property type="entry name" value="METALLOPROTEASE INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11844:SF33">
        <property type="entry name" value="TISSUE INHIBITOR OF METALLOPROTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00965">
        <property type="entry name" value="TIMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00206">
        <property type="entry name" value="NTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50242">
        <property type="entry name" value="TIMP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50189">
        <property type="entry name" value="NTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0481">Metalloenzyme inhibitor</keyword>
    <keyword evidence="4" id="KW-0483">Metalloprotease inhibitor</keyword>
    <keyword evidence="5" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040325366" evidence="10">
        <location>
            <begin position="22"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="NTR" evidence="11">
        <location>
            <begin position="22"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared with metalloproteinase partner</note>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="22"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="25"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="35"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="146"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="180"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR601820-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR601820-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8046403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="270" mass="31405" checksum="E146FB564F38DEBB" modified="2023-09-13" version="1">MQTYLLILVMVTAVVVPTTQACGFCHTYHPQQHYCQSDFAVRIEVVDIARKSDLVSYNLIGARIIDPMKMTSNKPGDIIKFYRPSSFCGTRFIKKHDYAITGSIKKKSDGSKYMIHGSCDFGVEWDEMSEQQQKGFEKKYARLCECKIHGVTGPMAIKVDSMKPKNTYPDARTYWTPNRCYFNPFQTRVFDDIDDCEEEYGFCFPENNGRCSWQLAPDYKECFEYRDNYVIARSGKFAITDPGQCKVLRSRRRRRTCRKRAKKYLESVES</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CKU7</accession>
    <name>A0A9Q1CKU7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">LRAT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_05060</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CKU7"/>
    <dbReference type="OrthoDB" id="421951at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1720.10">
        <property type="entry name" value="endopeptidase domain like (from Nostoc punctiforme)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007053">
        <property type="entry name" value="LRAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46137:SF11">
        <property type="entry name" value="LRAT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46137">
        <property type="entry name" value="OS05G0310600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04970">
        <property type="entry name" value="LRAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51934">
        <property type="entry name" value="LRAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="217"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="244"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="289"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="333"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="domain" description="LRAT" evidence="3">
        <location>
            <begin position="97"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="367"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="371"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8046404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="558" mass="64516" checksum="0D120525B13FD858" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CL04</accession>
    <name>A0A9Q1CL04_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">NTR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_05435</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CL04"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008191">
        <property type="term" value="F:metalloendopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002020">
        <property type="term" value="F:protease binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051045">
        <property type="term" value="P:negative regulation of membrane protein ectodomain proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001820">
        <property type="entry name" value="TIMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008993">
        <property type="entry name" value="TIMP-like_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11844">
        <property type="entry name" value="METALLOPROTEASE INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11844:SF25">
        <property type="entry name" value="NTR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00965">
        <property type="entry name" value="TIMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50242">
        <property type="entry name" value="TIMP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="3">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared with metalloproteinase partner</note>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="26"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="28"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR601820-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR601820-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8046673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="143" mass="16350" checksum="D5717F1D92F81544" modified="2023-09-13" version="1">MYVKMQDTYNVFFFVILMVCGKTFGCLCPREEDTRAFFCKQDIVFQGRVMSTVGLLQTESIHDNILMYKVKVEKMFLGYLGPEVWLSTSNGGSSCGVSLDENVSYLLTTSWDKRLITCGWNTPMTSLTEDDHYVINRKSDCDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CLK6</accession>
    <name>A0A9Q1CLK6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Isoleucine--tRNA ligase, cytoplasmic</fullName>
            <ecNumber evidence="4">6.1.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Isoleucyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">HOLleu_05769</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">Nanhai2018</strain>
            <tissue evidence="21">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>tRNA(Ile) + L-isoleucine + ATP = L-isoleucyl-tRNA(Ile) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:9666"/>
            <dbReference type="Rhea" id="RHEA-COMP:9695"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58045"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78528"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.5"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="15">Part of a multisubunit complex that groups tRNA ligases for Arg (RARS1), Asp (DARS1), Gln (QARS1), Ile (IARS1), Leu (LARS1), Lys (KARS1), Met (MARS1) the bifunctional ligase for Glu and Pro (EPRS1) and the auxiliary subunits AIMP1/p43, AIMP2/p38 and EEF1E1/p18.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 17">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.5" evidence="4"/>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CLK6"/>
    <dbReference type="OrthoDB" id="1706657at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017101">
        <property type="term" value="C:aminoacyl-tRNA synthetase multienzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004822">
        <property type="term" value="F:isoleucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006428">
        <property type="term" value="P:isoleucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07961">
        <property type="entry name" value="Anticodon_Ia_Ile_ABEc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00818">
        <property type="entry name" value="IleRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000414">
        <property type="entry name" value="Isoleucine--tRNA ligase, cytoplasmic-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.730.10:FF:000004">
        <property type="entry name" value="Isoleucyl-tRNA synthetase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000050">
        <property type="entry name" value="Isoleucyl-tRNA synthetase,cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02003">
        <property type="entry name" value="Ile_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033709">
        <property type="entry name" value="Anticodon_Ile_ABEc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002301">
        <property type="entry name" value="Ile-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023586">
        <property type="entry name" value="Ile-tRNA-ligase_type2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057033">
        <property type="entry name" value="Ubiquitin_IARS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00392">
        <property type="entry name" value="ileS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42780:SF1">
        <property type="entry name" value="ISOLEUCINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42780">
        <property type="entry name" value="SOLEUCYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19302">
        <property type="entry name" value="DUF5915"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23567">
        <property type="entry name" value="Ubiquitin_IARS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00984">
        <property type="entry name" value="TRNASYNTHILE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="ValRS/IleRS/LeuRS editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="12 17" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="9 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 17 21" id="KW-0436">Ligase</keyword>
    <keyword evidence="8 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10 17" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="18">
        <location>
            <begin position="18"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="domain" description="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding" evidence="19">
        <location>
            <begin position="694"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="domain" description="Isoleucine--tRNA ligase cytoplasmic ubiquitin-like" evidence="20">
        <location>
            <begin position="1093"/>
            <end position="1180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00032665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00063494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00069879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU363035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF23567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAJ8046925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1183" mass="136420" checksum="60C0B3690AB72062" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CMX7</accession>
    <name>A0A9Q1CMX7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_06342</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CMX7"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8047359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="77" mass="8829" checksum="CC6ED6A2AB3B37AE" modified="2023-09-13" version="1">MESSREFNFNAAFLELSRTNCLTPFLHCVLRLLSSIAYLAIRSWNQFLRYSTLQVYHCTIVNVHALPALGPDQANIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CN33</accession>
    <name>A0A9Q1CN33_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">B box-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_01152</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CN33"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19756">
        <property type="entry name" value="Bbox2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="B box-type" evidence="3">
        <location>
            <begin position="124"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="175"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="280"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8048722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="522" mass="58115" checksum="7A1828FF48A30EA5" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CPH2</accession>
    <name>A0A9Q1CPH2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Platelet glycoprotein V</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_00342</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CPH2"/>
    <dbReference type="OrthoDB" id="27267at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001360">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050541">
        <property type="entry name" value="LRR_TM_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369">
        <property type="entry name" value="ANTIGEN BSP, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369:SF210">
        <property type="entry name" value="CHAOPTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="425"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="domain" description="LRRCT" evidence="5">
        <location>
            <begin position="343"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8048154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="453" mass="51710" checksum="4F4DC736992AF5C8" modified="2023-09-13" version="1">MNLTISSELGDEMSGRSVDSLIIANTEASFIKTGNWNFLSHLTHLKQLSFYNNNITNISDFKSMYTKNLSVLETLTIARNKLTFFPVEKFIMLKSLKYLALYCNQLQTLGEREWSLPLLETLHLNINQITRVSEGQLKGLTSLKKLYLNNNRITYFSLKFFDSVPNLQYVNLSRNSLFCFSEYNVLHTSLFSINLESNFFETLNPVSFAGFKSLEFILMSNNLITHPPNTTQDVNVTTTIRLSFRRNKIEALSPLFFDNFPNMHSVAVTNNRISRIADNTFRGVVNLVSLDMEYNEIHTIPANLFRHLHQLSFIGLAHNRINILEPDIFTRLSKQVDLILYHNPIICNCSAVPLLRWLNKATMSRADHPRCWSPKSLRNQAIHLAELSNNCSDHETIKDSSLVITADTNPIIPTTPLVLTIKSRLILKLIGVGLGIGSLWYCINYINVVLGRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CQS4</accession>
    <name>A0A9Q1CQS4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Insulin-like growth factor-binding protein complex acid labile subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_02074</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CQS4"/>
    <dbReference type="OrthoDB" id="7451790at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.10">
        <property type="entry name" value="Tumor Necrosis Factor Receptor, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366">
        <property type="entry name" value="IG(IMMUNOGLOBULIN) AND LRR(LEUCINE RICH REPEAT) DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01411">
        <property type="entry name" value="Ephrin_rec_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="503"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="538"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="609"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="658"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="720"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="866"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="890"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="745"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="764"/>
            <end position="777"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8049360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="950" mass="109279" checksum="DF4A25EE3CEA65A3" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CQT5</accession>
    <name>A0A9Q1CQT5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Sulfotransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_01819</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CQT5"/>
    <dbReference type="OrthoDB" id="10019582at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053259">
        <property type="entry name" value="Golvesin-related_Golgi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005331">
        <property type="entry name" value="Sulfotransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32301">
        <property type="entry name" value="COUNTIN RECEPTOR CNR3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32301:SF6">
        <property type="entry name" value="GOLVESIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03567">
        <property type="entry name" value="Sulfotransfer_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8049193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="386" mass="44934" checksum="CF450EBD3D9F6548" modified="2023-09-13" version="1">MSGYHVATCSCSWSFQGIFACLVVVTVYSTYFGYSSYRTSVNVRLMQPDYANISSTLFMERKITTATVNESRNECHIVHEKLNSCNKLPNFFLYEDQRHHFQNAIINFWHLQKASGSTISHCLIDIYKKLGLPLTAHGEWSCKGKQKMLQNAYYYNTPNGRPVVRGHGTLGLCDFIKEKITDRKCSVFTVFRDPYDRLVSHYFFSRQIEKLKAPSHQSALKLNQTIKEWIRGSGSAVWQAFSNEWKFERINDGKEKCKGLQHFAVMSIEERWPSIDKHLTDISKNLDKHLSFIGLTEDLQTTYRMLEEVYGLPFVEVCSDMHVWQGSYEHTNATQQSALKMAAKREIMEDPEIQRLIQFEVLMYEKAEEIFKAQKRVFLKMIGSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CRA2</accession>
    <name>A0A9Q1CRA2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_02086</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CRA2"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040150896" description="Secreted protein" evidence="1">
        <location>
            <begin position="25"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8049370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="65" mass="7380" checksum="EE070D31A87A6937" modified="2023-09-13" version="1">MKIVLSQRITYLVTFAVVLGQTYALSTNACVFCNCSQEETMYVVNCSQKMFEEVPRFIPSNVGYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CRG3</accession>
    <name>A0A9Q1CRG3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_02220</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CRG3"/>
    <dbReference type="OrthoDB" id="9909359at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31635">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31635:SF196">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8049460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="230" mass="27023" checksum="CC3CD4D6B6C45F7A" modified="2023-09-13" version="1">MKTMNGYDVCLVIDDQLFFETLLCNIRGKSISYSSYKKRVTSEREKFLIEEIDWMEKNTLANSWRLNYVDDLKKELQEIRLNKVNGILLRSRAQRIDEDEKPSKYFLDLQKRNYEEKLISLLKIDNDEVLYANDGIKAEVYNFYKNLYRNNDGNVIDLNLEQGIINKLKILSDSESQDLEGPLGHLEVYNALKDMKNGKSHGPDGFTVEFLKYFWDKIGCFLVRSLIIFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CSG1</accession>
    <name>A0A9Q1CSG1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Immunoglobulin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_02474</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CSG1"/>
    <dbReference type="OrthoDB" id="5982258at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071222">
        <property type="term" value="P:cellular response to lipopolysaccharide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051713">
        <property type="entry name" value="T-cell_Activation_Regulation"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25466:SF14">
        <property type="entry name" value="BUTYROPHILIN SUBFAMILY 2 MEMBER A2-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25466">
        <property type="entry name" value="T-LYMPHOCYTE ACTIVATION ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="386"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="domain" description="Immunoglobulin" evidence="13">
        <location>
            <begin position="14"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="Immunoglobulin" evidence="13">
        <location>
            <begin position="236"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="537"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="610"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="539"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="612"/>
            <end position="643"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ8049639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="785" mass="86851" checksum="06505FECD516D50A" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H1D0</accession>
    <name>A0A9Q1H1D0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_24663</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H1D0"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040493871" description="Ig-like domain-containing protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="40"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8031467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="270" mass="31029" checksum="37A132F9D89E85F8" modified="2023-09-13" version="1">MYRLLTIFILIGCCCVTCCQAESPCQGFYGATSDVRGLIGENISLICKVPTNCIRGYWKHDNTINPMWLLPEQYSGDTYLFRFKSLEGLNTLHMYNINERVEGFYTCLCDDYYGNRTTNACFNLSVHVEHCPISVQINGEKRVLDRCGIRKEEEMVHVEVNETITIKCDDEAERRTNCSHIGKRMSFTVKPSHHLCCFSCRPKNDKLKRIYNSVILNVMNKVILISTEKLYTEIFSSTKSHLIGDVTGNVSPDQRLMISNLLHIFIPYHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H1D6</accession>
    <name>A0A9Q1H1D6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SHSP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_29029</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H1D6"/>
    <dbReference type="OrthoDB" id="10060792at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06526">
        <property type="entry name" value="metazoan_ACD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001436">
        <property type="entry name" value="Alpha-crystallin/sHSP_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45640">
        <property type="entry name" value="HEAT SHOCK PROTEIN HSP-12.2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45640:SF26">
        <property type="entry name" value="RE23625P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040184203" description="SHSP domain-containing protein" evidence="2">
        <location>
            <begin position="26"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="SHSP" evidence="3">
        <location>
            <begin position="220"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="315"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="121"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="157"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="177"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="336"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="357"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8029593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="374" mass="43574" checksum="1BA3467653878EE8" modified="2023-09-13" version="1">MEPLAIKSVIALALCGSVLFTEIQGSTYHNSFFPLKQRFNSFPWDPPVSLLDFFRYPSHSIRGWRDPRDAFMKQRYHTAANDASNHVSRYRTSNCRHRDDDYQTQKMVTSSEQNQRDYMINQRSSDWNTRGRNQHSTSNNPHCQEQSSNKESNPQQFDRHPNIKPQENDVKRKGPQRSYQQQEPIHQFQRQPKVTNPPVRKQQPHSSRPSQPLKMEEIANLDVSGYFPDEITIEVKNNGQIIIKGLHVCDCEESCFEREFQRRVAIPKGINVRSLKAVFTPDSKLRIVGYHSQEQNTSDFKLPFYVSVESDESLWHSKQRNSDPTCGGQKRGIKLKQMDSKSKQIIEDHPEVRTDEQFEGEIDEDGVTIETDEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H1Q5</accession>
    <name>A0A9Q1H1Q5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_25220</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H1Q5"/>
    <dbReference type="OrthoDB" id="6416765at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8031872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="301" mass="33950" checksum="C5A7AC13CFF9A52B" modified="2023-09-13" version="1">MPFPFVDPSLGYHASAAYLRQLLDRIEQDIIMPIVISSTEATPFRELLEVVVGTYNEELHKKGKRLDFGDDSTRWAYVYVFLMRHSHLVHTALVSMIPFTQKFAIWQNIPQLRMVAIGGGPGSDVLGFALFLKTLGITSNFHATIIDRCNEWKKTWASLRDRTQDLPISQVDFISEDLTSDGLSSHAREAIKHAHLITLVKVISSISAFIITKPVPQRLAFLETVTPGTYVLYIDNSVGPHTDLFMETIQYARLGFKLVNAFTNDVEFPLGPSSNQPLVEWLSFYPAKCGMNNFILLQKVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H392</accession>
    <name>A0A9Q1H392_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_27060</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H392"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8030601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="148" mass="16588" checksum="6AF9B2995BCE20AB" modified="2023-09-13" version="1">MDCLMKAYHGGVMSLQEYLHRILPELINQELTVNPRKGFQLFLSKTVVGLHMSNSVQKRTVVCARSETDEQTQVVQEVVHELLKSGRSNVLTLGLPKSLPCDAGLSFHSDHNYPQLITPHWSKLLQMCVAFNLLYTEKVFFLGGGGGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H3T7</accession>
    <name>A0A9Q1H3T7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Zinc finger MYM-type protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_25175</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H3T7"/>
    <dbReference type="OrthoDB" id="6602803at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025398">
        <property type="entry name" value="DUF4371"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45749">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45749:SF21">
        <property type="entry name" value="DUF4371 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14291">
        <property type="entry name" value="DUF4371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4371" evidence="2">
        <location>
            <begin position="248"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8031843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="508" mass="57226" checksum="415B927290D852AC" modified="2023-09-13" version="1">MGPHRPDTDRPKADEHEEQSPITLDESEHNDENPCSSDEWTDSVAGLSRIAQLADECKRILNDMKKADIPNVEFWMVEARETVSCIQENCSQFLETTDKVLQLVQADKRPVHQGSIKSSIEHDPALRGQEKADPAWVDLGVRTWHKMKSLGKDTYGRLAKHFASESHRASLSAYCSYINKNWHIDVQLDKATRNARIQEAEDLAFNRKVGSGDDKDGNYYQIVQLLGRHVPILRRWLSDRRSMNPYHVTYLSPQSQNELISLLETELCQRVVEEVHAAGMFSVMADTTPDESHTAQLSVVVRYVTESGKPVERLLAVNESLDKTGKGQAGEIVSAMDKLGLDTAELCYQSYDFAAAMSGQFSGAQKELSDIVGRKILYIPCQAHRTKRFQPLKDQVQTVEKGLMLRNLSKTRWSARAESIQAVWTSFETIVAVLGDIGGNPKIDKMTQTKAFGLKKRILDLDFIVTIMFMKNIMYKSKNLVEKLQKVDVNVLDAAALAEATMKVLDQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H6A1</accession>
    <name>A0A9Q1H6A1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_21140</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H6A1"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44592">
        <property type="entry name" value="NUDIX HYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44592:SF3">
        <property type="entry name" value="SECRETED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="93"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8034348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="297" mass="33709" checksum="EDF57208DAB708A3" modified="2023-09-13" version="1">MLVVTMTMTMTMTMTMTILSLLLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSVSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLLCHCHCHCHCHCHCHCHCHCHCHCHCHCHCHCHCHCHCHCHCHCHCHCHCHCHCHCHCHCHYHYHYHYHYHYHYYYYYYYYYYYYYYYYYYYYYYYYYYYYYIVIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H6H1</accession>
    <name>A0A9Q1H6H1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Calcyphosin-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_22019</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H6H1"/>
    <dbReference type="OrthoDB" id="444540at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040451761" evidence="1">
        <location>
            <begin position="23"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8034964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="131" mass="14667" checksum="50871F816C2B19D3" modified="2023-09-13" version="1">MNDRKVTLNLLLCLVLLQTVISEDNGEDTVSQAHRNILEMVFKEVDKLTDDGIITIDDAINALNVPSSITADGSMTDVEYLTNLLHQFEKEGIIDGQLTLEEYINSQASLSANIPENGLFYRLVRQKWGLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H6H2</accession>
    <name>A0A9Q1H6H2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_21445</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H6H2"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8034558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="129" mass="14018" checksum="B07E11D181E4C520" modified="2023-09-13" version="1">MIKGAASQEYLSGLEQAGLRRSSCSYAEASKRVFSNKIRERGSPYSDSTDENTTARHGHPQLVLNESGAAQSSCPTRAETSDQNTCRGNSSQFQVHLLNIVGTAYKLQTVLDSSKTVGAQVKWGECQFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H6J8</accession>
    <name>A0A9Q1H6J8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_21066</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H6J8"/>
    <dbReference type="OrthoDB" id="120976at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312">
        <property type="entry name" value="NACHT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312:SF2">
        <property type="entry name" value="NUCLEOTIDE-BINDING OLIGOMERIZATION DOMAIN-CONTAINING PROTEIN 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="454"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="468"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8034290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="484" mass="55941" checksum="3C2AF7B9C9CB7B7A" modified="2023-09-13" version="1">MQDTDIMHIIRKDMFQQIPVILTTRSSCIPHDLAANTRHIRLTGFDELARDRYIRKAVVGTDVKAAMKIKQRLKENPVLAGVCQVPLFFVMFAHMTHERHEFTQFQSVTSFFRFMIGCFHGHLHKKDKTKIRNFKSFEKDHGLLDQEALNSLSGGSKQNVWRKETLSELLGDKFYEHYVRVGILVEEEVLDFAEENGSSSSYYQPYRKDVRFYHKLFCEWYAAHYLSNIAAKSLSPSLELILSRLDPFELHYLYRFACGLNQNAANNILQYLSGVESSEQFEILCILEQRGQVENIKNQVQKLCSDTIIVSDEDSQLQQKSTIELLEIASSNAILISCVSVENCIKSVDLETWNLNLKSGMRLSNLGSVQEISVTQMGRELTEQEVLDIIRFCFQCLELKVLRFDCCLLPQYIKVTHTESDINQTNIKVIWSCTESWLQLNLTSGYWEIQSEGKSTPITDDDYQSESAKFRREWSGPKARNDKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H8W6</accession>
    <name>A0A9Q1H8W6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Voltage-dependent calcium channel gamma-7 subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_17619</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8036945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H8W6"/>
    <dbReference type="OrthoDB" id="9990458at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032281">
        <property type="term" value="C:AMPA glutamate receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098839">
        <property type="term" value="C:postsynaptic density membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016247">
        <property type="term" value="F:channel regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005245">
        <property type="term" value="F:voltage-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099590">
        <property type="term" value="P:neurotransmitter receptor internalization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098943">
        <property type="term" value="P:neurotransmitter receptor transport, postsynaptic endosome to lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051968">
        <property type="term" value="P:positive regulation of synaptic transmission, glutamatergic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098970">
        <property type="term" value="P:postsynaptic neurotransmitter receptor diffusion trapping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019226">
        <property type="term" value="P:transmission of nerve impulse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051072">
        <property type="entry name" value="CACNG_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12107:SF0">
        <property type="entry name" value="STARGAZIN (MAMMALIAN CALCIUM CHANNEL) HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12107">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL GAMMA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13903">
        <property type="entry name" value="Claudin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="119"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="149"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="194"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8036945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="294" mass="33297" checksum="F7C00730A0AC6DAE" modified="2023-09-13" version="1">MENRKLLALTVWTVVGSYVAFLMMCTATFTNHWIYTREIVKITNDTGIYSRDTQQHSGLLVTCQFDDKIHWNPHEGENSSWDAPLSENECRNLKKVLSNQDNPPDTITFAVFVHTSKSFAVPLMTCFLLLSAVIVCTVGQFMPKRRCMVFLSGSICVVCGLTSLTAIVRFVIAVNDAIIEKHSQSNDVIFQHHYGFSFVLTVLTFLLTNGVGVASLHLFILLERQAARSEKERVRHHLQRLGMNVVRTADESASFNNPEQGNERYGLPVSSWKETQYGFEGEPNWKDDRAKSEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H976</accession>
    <name>A0A9Q1H976_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Tenascin-R</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_14826</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H976"/>
    <dbReference type="OrthoDB" id="9990035at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050373">
        <property type="entry name" value="Fibrinogen_C-term_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020837">
        <property type="entry name" value="Fibrinogen_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143">
        <property type="entry name" value="FIBRINOGEN/TENASCIN/ANGIOPOEITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00514">
        <property type="entry name" value="FIBRINOGEN_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="2">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8040512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="87" mass="10103" checksum="052E9FD7669A3B82" modified="2023-09-13" version="1">MGHDYMSFHHEAYFNTFDSDHDDEPHQNCAMNYKGGWWYKNCYDANLNGIYGLTNGDWTGICLYNRDTTGAICDITFVEMKLKEVMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H992</accession>
    <name>A0A9Q1H992_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Major facilitator superfamily domain-containing protein 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_18615</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. MFSD6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H992"/>
    <dbReference type="OrthoDB" id="10056177at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024989">
        <property type="entry name" value="MFS_assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051717">
        <property type="entry name" value="MFS_MFSD6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16172:SF42">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16172">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 6-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12832">
        <property type="entry name" value="MFS_1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="175"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="220"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="257"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="301"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="332"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="365"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily associated" evidence="7">
        <location>
            <begin position="36"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8037724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="437" mass="49582" checksum="2B8579D318B0A2AF" modified="2023-09-13" version="1">MKFNEERKRKPNCNSTLERRRWLTLSVNINKTFLPVKLTYYLYLSAWAVIKPYLPIFMYELGMNAAQIGILRAIEPVTNFVASPVWGTVADKFNIHKMLMLACVIGSSICISLFVFVPNVTNLDEDRHSTEEFLPLMPTNIGVSGSRENLSFTTVSSQTSQKENNPSKLFPRDSLLSFILCALITTTENIFDAGFMPLFDSNTMELCRYYPGNTYGGQRAMGALAVGVISPFVGYLFDAYKYIIDAFSFSLLGNSWVPFFFMYTVVMLLTLIPVSKLKVVPMQPPESFSKEVIQLFRDRQIFITFLVVVVCGISYGIAGAFLLLFLEELHASRLLMSLTLVMTCAPETICLMFSGRIIKALTYEGVFCLGLFAYAIRFLGYSTIPEKATWVVLPVESLHGMFWTNLAQLYRICKLHCPRRNVSHTSVHYELSAWRSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H9W6</accession>
    <name>A0A9Q1H9W6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">F-box only protein 42</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_19046</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8038070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H9W6"/>
    <dbReference type="OrthoDB" id="9973021at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990756">
        <property type="term" value="F:ubiquitin-like ligase-substrate adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22110">
        <property type="entry name" value="F-box_FBXO42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052821">
        <property type="entry name" value="F-box_only_SRC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46432">
        <property type="entry name" value="F-BOX ONLY PROTEIN 42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46432:SF1">
        <property type="entry name" value="F-BOX ONLY PROTEIN 42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="9"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8038070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="449" mass="50720" checksum="7BB2B6415AD7E40A" modified="2023-09-13" version="1">MASSGCKVVLNINDLPTELLHSIFTYLSLYGQLQTSMQVCHRWKAIAEDVLKGREELFSCRLRENRLSFNTAGCPYISIPARSDHACCIHGKHLYIFGGFKNEGQAYNDLWKVDLIFREVERVQTTSKERGPSPRGYAFLLPYDANSLILCGGIVETKTPFAPTMYQRAADEVFKFDIKSNTWTLFKTNQGTSNQPENAIYDCCIVGRYLFACYKKKEEGQLCCQIQVINLSHSSNVACIPAETFRVPEMKLFSLDDFHLLVIANHLTKRECSAYLLDIRGFEEGTSLKWRDLEILNNDKCLPNPCQACKVGDHIVVFYPKEGTLLINAQQRVFAAKNYSRSPTAHKHCPHCTQAVHASWKGRCIFKDCDRLGTALQTAVMDVSCAVTSGVIKWVNSHIEFSGSTYFSRGQTHFRPFHAISGMAQVVILGWKPCPQKDCKHPCSIDVLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HAW0</accession>
    <name>A0A9Q1HAW0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Collagen alpha-1(XXVII) chain B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_16076</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HAW0"/>
    <dbReference type="OrthoDB" id="8939548at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005581">
        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050938">
        <property type="entry name" value="Collagen_Structural_Proteins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001791">
        <property type="entry name" value="Laminin_G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048287">
        <property type="entry name" value="TSPN-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37456:SF6">
        <property type="entry name" value="COLLAGEN ALPHA-1(XXIII) CHAIN-LIKE ISOFORM X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37456">
        <property type="entry name" value="SI:CH211-266K2.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00282">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00210">
        <property type="entry name" value="TSPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0176">Collagen</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040144630" evidence="4">
        <location>
            <begin position="30"/>
            <end position="1086"/>
        </location>
    </feature>
    <feature type="domain" description="Thrombospondin-like N-terminal" evidence="5">
        <location>
            <begin position="42"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="6">
        <location>
            <begin position="106"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="293"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="433"/>
            <end position="1086"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="340"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="357"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="367"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="567"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="618"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="849"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="898"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="909"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="973"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1003"/>
            <end position="1012"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="1013"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1060"/>
            <end position="1075"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8038611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1086" mass="107514" checksum="DC77DAED28424F39" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HB40</accession>
    <name>A0A9Q1HB40_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_16613</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HB40"/>
    <dbReference type="OrthoDB" id="10070527at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8039028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="111" mass="11969" checksum="93442A7CA5EFF0E9" modified="2023-09-13" version="1">MAEGGKKEKHLHQLAQTHQQVPAVTQNGNAQGGDISAALLAIMEMLECLEQGGAACDKKASPKKGSFNFYRCGQGGHIARGCKNEPTPEWTQRQLNRKLGKHSARGQAVEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HC65</accession>
    <name>A0A9Q1HC65_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_15041</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HC65"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8040679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="143" mass="16442" checksum="2DAB80CDDD2ECEC7" modified="2023-09-13" version="1">MQGSPEAAKPKGILKKRTTVSSRDSKMMRKPAKQKTKKTFKTTSKQIDKMIKKKTGGFVDKGLPQTEDKQAEGKKFPEKKQKEFHQKKGVKRKFEGGNKGGKGAKKPALETMTYKERKLVRKKTKNNYEISRQAKKIWETVRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HE83</accession>
    <name>A0A9Q1HE83_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Signal peptide peptidase-like 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_09578</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase A22B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8042735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HE83"/>
    <dbReference type="OrthoDB" id="29661at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098554">
        <property type="term" value="C:cytoplasmic side of endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030660">
        <property type="term" value="C:Golgi-associated vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098553">
        <property type="term" value="C:lumenal side of endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042500">
        <property type="term" value="F:aspartic endopeptidase activity, intramembrane cleaving"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033619">
        <property type="term" value="P:membrane protein proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007369">
        <property type="entry name" value="Peptidase_A22B_SPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006639">
        <property type="entry name" value="Preselin/SPP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12174">
        <property type="entry name" value="SIGNAL PEPTIDE PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12174:SF22">
        <property type="entry name" value="SIGNAL PEPTIDE PEPTIDASE-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04258">
        <property type="entry name" value="Peptidase_A22B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00730">
        <property type="entry name" value="PSN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="27"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="91"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="115"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="157"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="182"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="208"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="282"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="329"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="359"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="50"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="50"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8042735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="401" mass="44793" checksum="8CB05DB38D1C8A62" modified="2023-09-13" version="1">MNRQDRLEAVAMTEYDWAYSLVDSSRVSTFLISILLIVYGSFRSLNIDQEERERKEREEDRDNLLPTPPSGQNAAGQPGTENGVVIDTSQAMFLPIGASVSLLVMFFFFDSMQMVFAVCTAVLATVAFAFLLLPMCQYLMRPCSTGTKISFGICGRFTSAEIMSFCLSVMLVFMWVMTGHWLLMDALAMGLCVTMIAFVRLPSLKVSTLLLTGLLIYDVFWVFFSTYIFNANVMVRVATRPADNPVGVIARRLDLPNVAKDAPKLSLPGKLIFPSMHSAGHFSMLGLGDIVMPGLLLCFVLRYDNWKRQTAEPALPPAMIPHGSLAQKVTYFHCSLIGYFVGLLTATVSSEVYKNAQPALLYLVPFTLLPLVIMAYLKGDLKRMWHEPFIKTKSNNPYQEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HEE1</accession>
    <name>A0A9Q1HEE1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_10204</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HEE1"/>
    <dbReference type="OrthoDB" id="5978043at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8043214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="123" mass="13574" checksum="070D220DB7D414E3" modified="2023-09-13" version="1">MRDREEVSTSDKSSDSDNPTSDGQSSWPVYTIKGAHEIGDVNKPLMAKVKVNGKSLNMEIDTGSGKSIISYQEYRQKFPKVKLRPVSVVLKTVTGQKIDLCGTISVKVEYNQMCVTPYGDKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HFH4</accession>
    <name>A0A9Q1HFH4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_10146</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HFH4"/>
    <dbReference type="OrthoDB" id="5980337at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8043171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="261" mass="28708" checksum="7CEA88410029DF58" modified="2023-09-13" version="1">MATKEIPEKISFGILGCIQMFPLAKIVTLLDQGHGLLERFLITVPLCLRPTPTQTKEAKERLSELPPVSYADIGSLLCEKLTPGTEFHFSDEASELINKLDEDFINEINEAISNGHVPPKSKRIDLLVRVSVAIHIITILTTSLLDGVSEELLNITSEIPLSIVEAALYYVDYCENQKAILSAFVMDIVAMVMDSPRTQPSSLAIKLATLLFPGKAVTYRSLRGSGSRSVRGITVQEFETAIDDLKELGKVTQVRVPRSAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HI28</accession>
    <name>A0A9Q1HI28_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein cereblon</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_05224</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CRBN family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HI28"/>
    <dbReference type="OrthoDB" id="267517at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15777">
        <property type="entry name" value="CRBN_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.150.20:FF:000007">
        <property type="entry name" value="Protein cereblon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.40">
        <property type="entry name" value="LON domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.20">
        <property type="entry name" value="Peptide methionine sulfoxide reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034750">
        <property type="entry name" value="CULT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003111">
        <property type="entry name" value="Lon_prtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046336">
        <property type="entry name" value="Lon_prtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004910">
        <property type="entry name" value="Yippee/Mis18/Cereblon"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46732">
        <property type="entry name" value="ATP-DEPENDENT PROTEASE LA (LON) DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46732:SF8">
        <property type="entry name" value="ATP-DEPENDENT PROTEASE LA (LON) DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02190">
        <property type="entry name" value="LON_substr_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03226">
        <property type="entry name" value="Yippee-Mis18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00464">
        <property type="entry name" value="LON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51788">
        <property type="entry name" value="CULT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51787">
        <property type="entry name" value="LON_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Lon N-terminal" evidence="10">
        <location>
            <begin position="124"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="domain" description="CULT" evidence="11">
        <location>
            <begin position="368"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="9"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="28"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8046530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="487" mass="54732" checksum="CB7454D1ECEAC560" modified="2023-09-13" version="1">MDDSEEDNSDFHSADESLGHGEAQSGDSDAEDEAEDSEDEVEVNGGEAQNNGAGSDDQDSDDEDGFMYHVRMQIAGRVDVDTVTARPTMITYDPSLPTTHAYLGDDLEDHSGRTVLDDDSFVSIPLMQMHGVILTPGQTVPFHIFNQRSIAMLRSVIANDRTFGMLYDCKLSSGEVFVTNMGTTAEIISSKDEDEAGIGTMRLKAIGRQRFKVIESHRQLDGILMGKVLILEEKEVTDPLEGVRLKSYRARRVPPSSPRTSGTGFVEDAATWRRKRRKMQFHEAALSSLPSFVYEMYDSAMLMFHIKCELRSWYEGSMRLDAVPANAVDFSYWVASKMPLEDKQRIGLLQVDSAVQRLRAELNLLKQCTVLRCKNCRTHIGNKSDVFSMSLDGPMAAYVNPGGYVHETLTLYKAQNLNLLGRPSTENSWFPGFAWTILQCRSCAHHMGWKFTAVKKKYKPQKFWGLTRSALQSSLQDNEMEHFVLQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HI98</accession>
    <name>A0A9Q1HI98_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Microphthalmia-associated transcription factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_05476</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MiT/TFE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HI98"/>
    <dbReference type="OrthoDB" id="6242697at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11397">
        <property type="entry name" value="bHLHzip_MITF_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031867">
        <property type="entry name" value="MiT/TFE_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45776">
        <property type="entry name" value="MIP04163P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45776:SF2">
        <property type="entry name" value="MIP04163P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15951">
        <property type="entry name" value="MITF_TFEB_C_3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="8">
        <location>
            <begin position="279"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="153"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="238"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="304"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="476"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="154"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="171"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8046710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="495" mass="55706" checksum="087C02EB4881269B" modified="2023-09-13" version="1">MQESGIDLDFDINNLDIFNEEGNPADNLEKNNTELVVPFSDFNRFLKEFEGKTYELQSQVLQSETVNAPVTLNTMSTRTNFKLDLQRQQIAQEEAKERKLLENGAKPTTSAIDLPKTSLNTIPEVPHAVLKVSTQLENPTRYYIQERQKRQVQHYLSTSQEPSMSPHLVSPVTNSPSRLANGSQTSSLPNSPMSTQEIGDIFDDFGDGADEALLRLLQDTSIHMPNTMPSQGFIDSPTSASGVAPAAIPQQSMSCPPTIKQEPITLTEDQLRAYAKDRQKKDNHNMIERRRRFNINDRIKELGTLIPKSPDPDQRQNKGSILKNSVDYIRKLQREKDSHQKAEEKHRQLEVLSRRMLLRIQELEMKCKQHNVDVTPYSVETNTDALASQFIALNGQNLDMKIKMEPQEPPNTMSLDDFGLNTQPGTMVNPINQLPNQNSVLTSNPLAGNSNTMLDDMIEDSSSPVVSDALLWQNSPMASHHSSRRSSITSMEDLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HII8</accession>
    <name>A0A9Q1HII8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_09292</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8046108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HII8"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="93"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="127"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="201"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8046108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="213" mass="23219" checksum="459A4690E94F5B28" modified="2023-09-13" version="1">MRDAEVITALSEALKVPREDISNIKIFEGSIVVEATITSASESTEDKLIKMVEEGSFKVAFDGTEFTADSIEISETTMKPEETKTSPSSGRTVVFVLAGCFFALAVVAIISAACFVCKPKRRAPRFDSLNDNHGYDVPPVEYEPEEKGHDGKDSVEHGAVSNPYHRSLAFQLSEPLPSDTDKAKDEDVSNPYQRSIDAPNDGSSFVNEAFTTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HJ56</accession>
    <name>A0A9Q1HJ56_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_01295</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HJ56"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8048824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="67" mass="7837" checksum="E5E4127E00A8B8A3" modified="2023-09-13" version="1">MFNGAEMYQPDEIFYLKQALDAETLSPVIRDLSVRAFKGILRDSRSQDWEDRTFLTIALSCHRQKLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HKA3</accession>
    <name>A0A9Q1HKA3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_01484</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HKA3"/>
    <dbReference type="OrthoDB" id="6421164at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8048960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="164" mass="18900" checksum="046AC0F2A128B39E" modified="2023-09-13" version="1">MASFYVTLPSNSSPDVYPQNTLTNYRVKLAQPITLEGDWEVGLAEFIYPHQWYNIDEECKYSYTIDGGDHWLIKVIEPGFYDSIDSILHALKTDYNSLIQYHYNEYTRKLRINLLKGAQVKFRGRLAEILGFKGKTLLTESTTITHPVDIANISNLLVYCNINL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HKN3</accession>
    <name>A0A9Q1HKN3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_01128</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HKN3"/>
    <dbReference type="OrthoDB" id="9411774at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000508">
        <property type="entry name" value="Myeloid zinc finger 1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:003017">
        <property type="entry name" value="Si:cabz01054396.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000702">
        <property type="entry name" value="Transcription factor E4F1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001618">
        <property type="entry name" value="Zinc finger protein 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002716">
        <property type="entry name" value="Zinc finger protein 212"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000358">
        <property type="entry name" value="zinc finger protein 24"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000188">
        <property type="entry name" value="Zinc finger protein 787"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24381:SF393">
        <property type="entry name" value="CHROMATIN-LINKED ADAPTOR FOR MSL PROTEINS, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24381">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="22"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="21"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="357"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="400"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="428"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="511"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="539"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="567"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="595"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="623"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="651"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="679"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="707"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="735"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="763"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="791"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="819"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="847"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="875"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="903"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="931"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="959"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="987"/>
            <end position="1012"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="11"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="49"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ8048706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1012" mass="116670" checksum="B4F19AFE64F7E8F4" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HL38</accession>
    <name>A0A9Q1HL38_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Zinc finger CCHC domain-containing protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_02885</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HL38"/>
    <dbReference type="OrthoDB" id="3863715at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002218">
        <property type="term" value="P:activation of innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042509">
        <property type="entry name" value="ZCCHC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22639">
        <property type="entry name" value="GAG-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22639:SF3">
        <property type="entry name" value="ZINC FINGER CCHC DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="162"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="330"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8049920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="356" mass="38671" checksum="69656A6767757D01" modified="2023-09-13" version="1">MPGGRDSTIVKLEFSSGVSVPPPQAFTVLKDNGVETGEIEMLQALEARNTYDLKFKSDAARVKGVSCLKGVEGLTVTPYDGSVAVTVLYLPFEVDQRLVARVLSQYGTVSEMRWCKYTAGELKGIFNGKRQFRMELVRDIPSFLFIGGSKAHIRYFCQPRTCFRCGEEGHEAKSCPNRRCGKCLQLGHGKAECPNEVRCNLCGEVGHVFGSCPTSYSAHVSADVGSAPEPSQTGPQYSTQKLEEAALEVEQDFLASKQCEEGTGTPEADCPPSLAAAPGQVEIMTLEEDLDLSSDSAMSDGGPQSLLQRIRLRGRVGLICVRGRGQSALKRSASGEVSDRGTHTLGRPYRAHRLKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HLD0</accession>
    <name>A0A9Q1HLD0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_03463</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HLD0"/>
    <dbReference type="OrthoDB" id="10167998at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8050310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="143" mass="16209" checksum="D40B1E5E4A66DAA8" modified="2023-09-13" version="1">MIEFEISIQERNFRISAMVVKNLIGIDLILGSKTLAELDGILEFKTNTFRIAPKKLHLRPSAYVVIKPGQETYITLRGKVPPYLRNAEVIIQPNRFLSKFCPSQMLVKFKRGRTSIPVKNLSHKTIHVAPDKPLASLHFKSVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0Y8W6</accession>
    <name>A0A9Q0Y8W6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_44418</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000864">
        <property type="protein sequence ID" value="KAJ8017903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0Y8W6"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="1">
        <location>
            <begin position="32"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8017903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="251" mass="28284" checksum="A460128C093B0AB6" modified="2023-09-13" version="1">MVYSVVVIDNSYNTEEYKITFTTVVYTEQLGPRITDCTTGTFCHVYVEDLDNLRCSVFGARPAVHLEWFQKITENEPFLLASEYIHEPNELSSYLSDSSVILPGDRVPGTIPSVFVCKGTGPAIKSNMTFQQVLLVESKNFYDELENLTISSEKIINVSNKENVSLPCQTVTEESLIIWRRGSSVDKLHTILYSFYGNEVRDVNSETGINKTGHLFLFNVELYLEKVFVCIAMENNGSNVGQIFKISVDGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YCT8</accession>
    <name>A0A9Q0YCT8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_44135</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000659">
        <property type="protein sequence ID" value="KAJ8018074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YCT8"/>
    <dbReference type="OrthoDB" id="8960137at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19963">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19963:SF30">
        <property type="entry name" value="ENDONUCLEASE_EXONUCLEASE_PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8018074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="240" mass="26912" checksum="6099753523EC0DF4" modified="2023-09-13" version="1">MVAELHRWSGGVKAMCLAASLRGDAQAILADLEAEKRRNYTNLVDAISSRFGPARQTELRRIQLRNRGRKKGETIPDLAQDVKRLTRFAYPGASSHLQDQLSRDHFVDCLEEPELRLGVHQTRPKTLEEAVQAALEIEAFYTVERQRNFPLRKTVRVLHSDQGMTNTLAGANVSVLSRTFLLEFPEEKQVNCTEVNNKMSLADGKSVPLHGLLEAGVNIDGNLVQHKFWVADIDVECILG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YDA5</accession>
    <name>A0A9Q0YDA5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_44473</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000914">
        <property type="protein sequence ID" value="KAJ8017862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YDA5"/>
    <dbReference type="OrthoDB" id="416119at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8017862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="481" mass="54953" checksum="8D9F61A97A024987" modified="2023-09-13" version="1">MDIDQGATHQNSVELTDAVVEIQKAEVKACDSEAATNRNDDSVINTSESQETVVCDDHSQQYEDSQIWHTESLTDCSSQLSLPDTPESPSLFGDWAETGVTSAGGGASSADVPNNSSPGSKDSTLGTRCPNIERVTRTRKRSSSQSGTTKKKERSRSNIRFPQEDPSDLIKMTQFFLEDEPWHSSLARGCPATFSRFIHPQTPGHTWFRVNSNQSSRIDRIYVPKEVKVCNSETFCLLYFDHNPVYVDFVTPNTPDSNGKGYWKYNVSLNRDKDFCRDLRHHYQMWSSLKPGFDSLSDWWENIKGRIRELAIKHSKRRVRKKRERLNHLQAHCSAFTAEEIDNLIGTEAEGAYIRYRAKFLEFGERPSSYFFRLESRRGSQKIVRSIRGPNGDIFNRKDDIIRVFHDFYSSLYSKERNVDHGLQDSFIHSLKSTIDNNQKNKLDLPISSDEIYSALRGARLKTKRPVLTVSLMSFTLTFTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YDP6</accession>
    <name>A0A9Q0YDP6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ubiquitin-conjugating enzyme E2 G2</fullName>
            <ecNumber evidence="2">2.3.2.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">E2 ubiquitin-conjugating enzyme G2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Ubiquitin carrier protein G2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Ubiquitin-protein ligase G2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">HOLleu_41236</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">Nanhai2018</strain>
            <tissue evidence="16">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Involved in endoplasmic reticulum-associated degradation (ERAD). Required for sterol-induced ubiquitination of 3-hydroxy-3-methylglutaryl coenzyme A reductase and its subsequent proteasomal degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.</text>
            <dbReference type="EC" id="2.3.2.23"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Interacts with AUP1 (via C-terminus); the interaction recruits UBE2G2 to lipid droplets. Interacts with ubiquitin ligases AMFR/gp78 and RNF139/TRC8; recruitment to lipid droplets by AUP1 facilitates interaction of UBE2G2 with AMFR and RNF139, leading to sterol-induced ubiquitination of 3-hydroxy-3-methylglutaryl coenzyme A reductase and its subsequent proteasomal degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="2"/>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8019586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YDP6"/>
    <dbReference type="OrthoDB" id="19692at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd23796">
        <property type="entry name" value="UBCc_UBE2G2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000008">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 G2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 14" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="15">
        <location>
            <begin position="4"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="13">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00055690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00063420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00073285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00076340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00079258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00079959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAJ8019586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="167" mass="18580" checksum="D3EB6CCCE27C34E3" modified="2023-09-13" version="1">MAGSALRRLMAEYKQLTVNPPEGIVAGPVNEENFFEWEALIVGPEGTCFEGGVFPAEIIFPTDYPLTPPKMKFKCEMFHPNIYSDGRVCISILHAPGDDPMGYESSAERWSPVQSVEKILLSVVSMLAEPNAESGANVDASKMWRDERELFYKRAKEIVKKSLGYGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YDY2</accession>
    <name>A0A9Q0YDY2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_43320</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000268">
        <property type="protein sequence ID" value="KAJ8018599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YDY2"/>
    <dbReference type="OrthoDB" id="430476at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09272">
        <property type="entry name" value="RNase_HI_RT_Ty1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8018599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="152" mass="16769" checksum="B74984B7230BB390" modified="2023-09-13" version="1">MPDGGSRAGYLIMLAGYSGTFSPIWWNSKKIRRVVRSTLAAETLARAEGIDASIFICSLLGELVYGKPEANLFPIVCFTDCKSLHDVLKSPKFVSEKRLRLEISGIKEQLQKGQVKRVEWISSDLQLADCLTKKGAANNELRKALHSGVLTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YE41</accession>
    <name>A0A9Q0YE41_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_40782</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8021028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YE41"/>
    <dbReference type="OrthoDB" id="7975104at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040147601" evidence="2">
        <location>
            <begin position="22"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="117"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="136"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8021028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="299" mass="34845" checksum="61E4F8FA15865D39" modified="2023-09-13" version="1">MFTRVLVFIVVILSFGMFYRQSTIKEKGQEFLLGKHERLTLDAALDIARTHEATRMELEELANAGNVSVNYARQQLRLDTKVRFLQCRKCGLNPMEEDYPAKGTKCKIWGKLNHWKRVCKSKPKPQQPRERYRSNTNKHRGTNRRKRSVNELSNDQDPAKQNDQQETDTEVNVMHVDHNQSQTRDRDEILAKVEIQVPNESRRCTIKQATNEDQEIAALGEIINGWPEKQRSLRQGWSYLDDLTVEEGDVIMKEERLLITKQSRKDILAKQYDTDQGIVLCQLRGKWTVFGQKSTRTLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YEK5</accession>
    <name>A0A9Q0YEK5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_42724</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000125">
        <property type="protein sequence ID" value="KAJ8018974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YEK5"/>
    <dbReference type="OrthoDB" id="10051210at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331:SF6">
        <property type="entry name" value="DOUBLECORTIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8018974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="103" mass="11637" checksum="64CF4E4727253D14" modified="2023-09-13" version="1">MLLRVTSFKDKSQAEKIEYRMKVHLFGAASYPGRAIFGLHQAAKDGEETWGKEAAEFIPKDFYVDYGIKFVKSSKEAIPFINNSTGMCADAGLRLHKWSSNCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YF06</accession>
    <name>A0A9Q0YF06_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_39830</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MS4A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YF06"/>
    <dbReference type="OrthoDB" id="10071849at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007237">
        <property type="entry name" value="CD20-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030417">
        <property type="entry name" value="MS4A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23320:SF165">
        <property type="entry name" value="MARVEL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23320">
        <property type="entry name" value="MEMBRANE-SPANNING 4-DOMAINS SUBFAMILY A MS4A -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04103">
        <property type="entry name" value="CD20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="49"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="92"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="115"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="183"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="275"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="19"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="313"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8020276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="347" mass="37840" checksum="1C60741FD208E3B2" modified="2023-09-13" version="1">MHTTQNSPQVFTAGPGTWQPVNPGTTVSPVTSNEPARRGGASGRICSSIFQAILAVLLIVFGIIAVSLVIPDTSSIPFYRREGIDRRDRQSWGIWCGVLFFLTSVFGLSSKKNQVLISLYMAFSIVATLLAFCGIVASCIQLAIASNVKRDVEDYDLFFYNPVFNYNGDVQLEKVRKVLSAHLACYAIMLTCFVVEFIVCIVGAAFTCSGLDCCSGGSNQRVIYYQTQVPQQPVPYSPPQQFPVGVPLPAGTSMVQTTQTVHQGPPMATQFTPHNRYVTAPPPYQPETGPQVSTSKEMRIRPLPSAPPTYESTFTTDDTQGTQDNHGTQNSQGDQGSNDVDYLEVIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YFT7</accession>
    <name>A0A9Q0YFT7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_42093</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000030">
        <property type="protein sequence ID" value="KAJ8019362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YFT7"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8019362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="56" mass="6552" checksum="4E4636539F8924DC" modified="2023-09-13" version="1">MLSKDAEDRKGRTEETYKGWVKGGRELLRERADRKRAKLRDEALDDEDDPDDDDVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YGF3</accession>
    <name>A0A9Q0YGF3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Epoxide hydrolase</fullName>
            <ecNumber evidence="6">3.3.2.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_40707</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>1-(4-methoxyphenyl)-N-methyl-N-[(3-methyloxetan-3-yl)methyl]methanamine + H2O = 2-{[(4-methoxybenzyl)(methyl)amino]methyl}-2-methylpropane-1,3-diol</text>
            <dbReference type="Rhea" id="RHEA:55764"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:139161"/>
            <dbReference type="ChEBI" id="CHEBI:139164"/>
            <dbReference type="EC" id="3.3.2.9"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>cis-stilbene oxide + H2O = (1R,2R)-hydrobenzoin</text>
            <dbReference type="Rhea" id="RHEA:23900"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:50004"/>
            <dbReference type="ChEBI" id="CHEBI:50014"/>
            <dbReference type="EC" id="3.3.2.9"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the peptidase S33 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.3.2.9" evidence="6"/>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YGF3"/>
    <dbReference type="OrthoDB" id="7130006at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033961">
        <property type="term" value="F:cis-stilbene-oxide hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097176">
        <property type="term" value="P:epoxide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010497">
        <property type="entry name" value="Epoxide_hydro_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016292">
        <property type="entry name" value="Epoxide_hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21661:SF35">
        <property type="entry name" value="EPOXIDE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21661">
        <property type="entry name" value="EPOXIDE HYDROLASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06441">
        <property type="entry name" value="EHN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001112">
        <property type="entry name" value="Epoxide_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0058">Aromatic hydrocarbons catabolism</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 6 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="domain" description="Epoxide hydrolase N-terminal" evidence="9">
        <location>
            <begin position="52"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="7">
        <location>
            <position position="230"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="377"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR001112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001112-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF06441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8020966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="465" mass="53076" checksum="15EAF321394300CA" modified="2023-09-13" version="1">MGWKLQLFVVGIAIVGGLYLAGFFSPEPPNLDPGEGWWAKEDKGTVKVDTTIKDFKIVFPQKDQEDLKRRLENTRYFESLEGTNWEYGIRANEMKKIVNYWLTNYSWPKQEKLFNQYKHFKTNIEGLDIHFVHVKPNLKPGQKAKPILMVHGWPGSFYEFYKIIPMLTDPTSHGGTEDDIFEVICPSLPGYGFSEAAHKPGLIPKDIARMFNTLMHERLGFDSYYVQGGDVGGSVVQLMALFFPQYVRGHHNNFLDIQPPLLPIRLLVGSVFPSLVTKNEADQKKMFPIKDRLIKLLRETGYMHIQATKPDTLGHGLTDSPVGLAAYMLEKFSVWTNPSFIESPDGQLTKKFELDDLLNNVMVYWISGSITSSMRLYKETFAKYHEMTSYLSTTTSVPTGYASLPHEIACTPEPWLSYVFKNVISYTAFPDGGHFAAFEEPKLMTEDIRKFAKGVEDFLQQNSEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YGH4</accession>
    <name>A0A9Q0YGH4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_39404</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8022030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YGH4"/>
    <dbReference type="OrthoDB" id="9995764at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019534">
        <property type="entry name" value="DUF2452"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14553:SF1">
        <property type="entry name" value="SIMILAR TO CHROMOSOME 1 OPEN READING FRAME 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14553">
        <property type="entry name" value="UNCHARACTERIZED PROTEIN C1ORF50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10504">
        <property type="entry name" value="DUF2452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8022030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="114" mass="12855" checksum="3EB44318DF8117CE" modified="2023-09-13" version="1">MDPTSKAVSLVESESRPSGVQLVNTERTNKITDPMDLVALAEQVQRRDRIHVRPSPEPPSKDPAPRELPTPQPNSSPPTESMEGKTSLQTRGNPYIRPVRDRKPPERYKDYILT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YHC6</accession>
    <name>A0A9Q0YHC6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_39747</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YHC6"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8020217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="186" mass="20501" checksum="CECFA83EF3570F0E" modified="2023-09-13" version="1">MRVTCVGTQRIEVSEHTTQGCKCWNNGQVQANWIPPSEVKKCFQLFKNCSEQWRRERMEKNLGWLTIIPILAITQSPTPLHEIIDPCNPMENIVAMPTACKLEKLVALGRGTHGVGGNCVPSGPVAEIYSPSFLGCNGPTPLESFTHSNAIILISPKEVGQSFSRQGHSQRANGESNVPLHNHVYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YHI4</accession>
    <name>A0A9Q0YHI4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">RAD50-interacting protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_38781</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RINT1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YHI4"/>
    <dbReference type="OrthoDB" id="2189254at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070939">
        <property type="term" value="C:Dsl1/NZR complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060628">
        <property type="term" value="P:regulation of ER to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.670:FF:000003">
        <property type="entry name" value="RAD50-interacting protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1420">
        <property type="entry name" value="Dsl1p vesicle tethering complex, Tip20p subunit, domain B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.670">
        <property type="entry name" value="Dsl1p vesicle tethering complex, Tip20p subunit, domain D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042044">
        <property type="entry name" value="EXOC6PINT-1/Sec15/Tip20_C_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007528">
        <property type="entry name" value="RINT1_Tip20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042042">
        <property type="entry name" value="Tip20p_domB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13520:SF0">
        <property type="entry name" value="RAD50-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13520">
        <property type="entry name" value="RAD50-INTERACTING PROTEIN 1 RINT-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04437">
        <property type="entry name" value="RINT1_TIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51386">
        <property type="entry name" value="RINT1_TIP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00061158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8021540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="725" mass="83408" checksum="3C13DD5CFA572FD0" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YHS9</accession>
    <name>A0A9Q0YHS9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Interferon regulatory factor 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_37884</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YHS9"/>
    <dbReference type="OrthoDB" id="6538197at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00103">
        <property type="entry name" value="IRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000065">
        <property type="entry name" value="Interferon regulatory factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019817">
        <property type="entry name" value="Interferon_reg_fac_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001346">
        <property type="entry name" value="Interferon_reg_fact_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11949">
        <property type="entry name" value="INTERFERON REGULATORY FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11949:SF17">
        <property type="entry name" value="IRF TRYPTOPHAN PENTAD REPEAT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00605">
        <property type="entry name" value="IRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00267">
        <property type="entry name" value="INTFRNREGFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00348">
        <property type="entry name" value="IRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00601">
        <property type="entry name" value="IRF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51507">
        <property type="entry name" value="IRF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="IRF tryptophan pentad repeat" evidence="10">
        <location>
            <begin position="69"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="31"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="171"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="252"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="31"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="193"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="208"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="255"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8022867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="445" mass="50259" checksum="D095C6661C7C0711" modified="2023-09-13" version="1">MPGLVLPSPSHLSNFGQAMYHTHQGMSYTTAHLPTVPSGYNPNSTHQYPRQPSQPPQETQSKPLRPAERMRLRPWLIKMIEERKIAGLEWEDKERQIVRIPWKHAARHGWIKDRDASLFKAWAIHTKKYNPDTDTPKANPKMWKANFRCAINSLPDIEELRDRGKTKGNDAYKVYRLIPKTKAAKPKSDPAKSKKSRNSKPKALRAAKQTTDTKPNALLSSRQSEIPAPQRMDYIASQRLDSKLSISTKVENQFQSPLSSPTSVASSQPSTPLSAPPVSFPKQWSDPYPSWLQPTTFQQYTPEADEYMSDASGSNMTDEEIVDMVDEMIKESPTHSPNHTVPSSPLDFDGNNNNVLWENEVQRTLSQAFGQPLPSPISPTVTPTNCHTYYNPEYHHVGIVKVEPQSEDCLSPSSYPREPPPNYYDAVAARAECIKEESSPTYCKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YK26</accession>
    <name>A0A9Q0YK26_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Next to BRCA1 protein1 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_37777</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YK26"/>
    <dbReference type="OrthoDB" id="2122982at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070013">
        <property type="term" value="C:intracellular organelle lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02340">
        <property type="entry name" value="ZZ_NBR1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.90:FF:000007">
        <property type="entry name" value="Next to BRCA1 gene 1 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000433">
        <property type="entry name" value="Znf_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043145">
        <property type="entry name" value="Znf_ZZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20930:SF2">
        <property type="entry name" value="NEXT TO BRCA1 GENE 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20930">
        <property type="entry name" value="OVARIAN CARCINOMA ANTIGEN CA125-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00569">
        <property type="entry name" value="ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00291">
        <property type="entry name" value="ZnF_ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01357">
        <property type="entry name" value="ZF_ZZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50135">
        <property type="entry name" value="ZF_ZZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZZ-type" evidence="6">
        <location>
            <begin position="263"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="352"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="354"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8022785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="373" mass="41771" checksum="08878A82CC9FF2B0" modified="2023-09-13" version="1">MASSLALEFEFNKKSQICVVGYDTAWIDVYAMIKCSLDLDDIEIKYLDDENDEFRWLLKPKTLTEQMINLSSEEEYVQAREVARKSGDVLKFTVYDPKSGIQKEPVTQTRAVSKPTPNSRYDPVTGCALSGAKMSGKNEVKPLASISISQDTLGETKRPPDKFVNNIVQDILQGLDGSIEIDEETEEMKATQKKIRQGIKQKVQSTNIEWSPSCKRIPKKIAQPNEEFVNKVVQGVLSGLNGSEIRSAREAAQVDYGMNFHIHEGVTCDACKKAIVGIRYKCGHCPDFDLCEKCEASGTKHDENHVFLKIRKPAILGLGQRFDGQCIPLLEVPVYPTDENLTRWEQKKLKLRSENSSQNCQGGNETQVQGGET</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YKR2</accession>
    <name>A0A9Q0YKR2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_35527</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YKR2"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8023201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="248" mass="27147" checksum="0D997DEA7DD20090" modified="2023-09-13" version="1">MHSRRELRSRTSFLAHAELETGVAARKFRRCNEKSRAAARACLGKQPAFQTPHAELETGVAARKFRRCNEKSRAAARACLGKQPAFQTPHAELETGVAARKFRRCNEKSRAAARACLGKQPAFQTPHAELETGVAARKFRRCNEKSRAAARACLGKQPAFQTPHAELETGVAARKFRRCNEKSRAAARACLGKQPAFQTPHAELETGVAARKFRRCNEKSRAAARACLGKQPAFQTRTSAGLVGGQPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YLT9</accession>
    <name>A0A9Q0YLT9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cytochrome P450 20A1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_34942</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YLT9"/>
    <dbReference type="OrthoDB" id="1470350at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052666">
        <property type="entry name" value="CYP450_20A1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24280">
        <property type="entry name" value="CYTOCHROME P450 20A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24280:SF4">
        <property type="entry name" value="CYTOCHROME P450 20A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0349">Heme</keyword>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="2">
        <location>
            <position position="420"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8024895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="474" mass="54347" checksum="1E2B9033A6F2097E" modified="2023-09-13" version="1">MITTITFVAAFVAIVLVAFVYIRSKPSKAPTVPGLQPVDYKGKGNLPDIKAAGSLHEFLVKLHKQYGPIASFWFGPTFTVSVASPDILQDLKRIFDRPPELFRLFEPLFGPESIQYCNGTEGKTRRKTTDPAFSHDMLRHFCENFNKLGEEAVSKLASLPRDEHIPICQTTLALTIKGLSQTSFGAYFKSDDMCYKLQHNYELCWHEMERRVDGDIPESNSERQKRFIEALETIKGMVKDIVKLRQESPPQPHERGFIDILMDHTDIYPERKLLDDAMTYMIGGFHTSGHLLAWTLYFLAKHKDVQEKVFKEVISIVGKDGLIGPEDMREFVYIRQVMNESLRLSVLAPWAARFQEVDVHLGGYIIPKGTPVITALGVVLQDEAIWPLSNKFDPERFSEENVRQRHQFAFQPFGLPGRICPGYRFAFYELTVFLAILIRNFKWSLVEGQAEVERVHAFVTSPKEEIWTLIEKRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YM30</accession>
    <name>A0A9Q0YM30_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Neutral ceramidase</fullName>
            <ecNumber evidence="2 7">3.5.1.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_37588</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>an N-acylsphing-4-enine + H2O = sphing-4-enine + a fatty acid</text>
            <dbReference type="Rhea" id="RHEA:20856"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:52639"/>
            <dbReference type="ChEBI" id="CHEBI:57756"/>
            <dbReference type="EC" id="3.5.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="6">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the neutral ceramidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.23" evidence="2 7"/>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YM30"/>
    <dbReference type="OrthoDB" id="191371at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017040">
        <property type="term" value="F:N-acylsphingosine amidohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046514">
        <property type="term" value="P:ceramide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042759">
        <property type="term" value="P:long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046512">
        <property type="term" value="P:sphingosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2300">
        <property type="entry name" value="Neutral/alkaline non-lysosomal ceramidase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006823">
        <property type="entry name" value="Ceramidase_alk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038445">
        <property type="entry name" value="NCDase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031331">
        <property type="entry name" value="NEUT/ALK_ceramidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031329">
        <property type="entry name" value="NEUT/ALK_ceramidase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12670">
        <property type="entry name" value="CERAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12670:SF1">
        <property type="entry name" value="NEUTRAL CERAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04734">
        <property type="entry name" value="Ceramidase_alk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17048">
        <property type="entry name" value="Ceramidse_alk_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Neutral/alkaline non-lysosomal ceramidase N-terminal" evidence="8">
        <location>
            <begin position="54"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="domain" description="Neutral/alkaline non-lysosomal ceramidase C-terminal" evidence="9">
        <location>
            <begin position="564"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="307"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="494"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="533"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR606823-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR606823-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF17048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8022632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="729" mass="80842" checksum="53E377BAF03DC0BE" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YMQ8</accession>
    <name>A0A9Q0YMQ8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_38083</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8023022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YMQ8"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8023022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="198" mass="22650" checksum="CAB643142992408B" modified="2023-09-13" version="1">MKKWLGFGKPELRGAAEDDSYLTSELLDDDEISKIGVAQWNRSRLLLPSSKTKNVQQQRPSFLTNLKKWLGFSQTEPECDVYDTNESMCNPFTVQYLHEDLVRRETTSAHDDGYAYVRRLPSLMVPELPSIEKGASSSIRLSRRDRIRKMFEDVAQEKENSTDKAISQDLNFFPKRTTSTKGDPTGIQMTSKCFVSLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YPN9</accession>
    <name>A0A9Q0YPN9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_36732</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8024102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YPN9"/>
    <dbReference type="OrthoDB" id="6380096at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF1">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8024102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="287" mass="33367" checksum="068366DE87FD9CCB" modified="2023-09-13" version="1">MMMTRGKTTKDEVETAESGNEQTPHSIPEGLPKEMWVQYLIDMQKLQHEKDVKLQIEKLQHDRELEKIKAEKEIELRELEFSERSSSSSLHGNSQEISNMTYGYDIRRVPRLRDDDDIDVYLRAFELLAEGNGWDKSQWEKLLIPSLSGKAREVYANLSVADSQNYDVLKTAILAKYEINAESYRIKFRNTCRKPDETIAEWINDLSYFLDGWIKFSDIRQDSVQAVKELLIVEQAIGKFPDDLAIYIKDKQVKTAKDLAQAADQYVINRGGKKYWKHVGRKVALTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-10-08" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YQG1</accession>
    <name>A0A9Q0YQG1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_31450</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10064731at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09274">
        <property type="entry name" value="RNase_HI_RT_Ty3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22249">
        <property type="entry name" value="UDM1_RNF168_RNF169-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000115">
        <property type="entry name" value="Polyprotein of retroviral origin, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000032">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 297-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon gypsy-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054465">
        <property type="entry name" value="Integrase_p58-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041373">
        <property type="entry name" value="RT_RNaseH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22938">
        <property type="entry name" value="Integrase_p58_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17917">
        <property type="entry name" value="RT_RNaseH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="9">
        <location>
            <begin position="559"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase" evidence="8">
        <location>
            <begin position="973"/>
            <end position="1150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="94"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="344"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="346"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="362"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="386"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="408"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8026574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1364" mass="157935" checksum="450336B2AC5E49DD" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YRL2</accession>
    <name>A0A9Q0YRL2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_35181</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8025077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YRL2"/>
    <dbReference type="OrthoDB" id="6264873at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="188"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8025077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="252" mass="28134" checksum="E5322F3098C45FBC" modified="2023-09-13" version="1">MNNQENKIEELLPMIGLSTLDDFVQSPPPFLKLISTSPLQWAVNRNLVNICVDPKRHLYFRGAVGNVIKFETAEKFENIVKSLLSKPLVLEVNLEAGTRCRSIGVHYFVTVNTSHPVIKGQILDAVNGVRTRVTNEGPVVLKIDFNNVIDCWFREMPGYRGSKTSNTASRAEVVITSKPDVHPCFRGVVLGTGIIGGAIMLTFLLCCVTYYAVREIRTSNKKSRIQGEIKHLTWRNNVPVSSTDFAINFGEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YSM0</accession>
    <name>A0A9Q0YSM0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_32260</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YSM0"/>
    <dbReference type="OrthoDB" id="7339946at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47018">
        <property type="entry name" value="CXC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8027191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="433" mass="49121" checksum="8D0B51E674B91FC5" modified="2023-09-13" version="1">MVSLKQLNPVVYAEFLKGNFVVKKSKGAFSAVAIDQAHEQNNASMKGDGVVVSLPENPADLRCWMVSGPEMARLIQEFEGSTEKRKDTDGRHHEQKRHSQMEFAQDLGSLCQAMNEMGNPFAEYSSDLLVLNSRDVVDTAVADTVLQMEKVGLEQYETYVEERLVNQTVPSTDPIKRNNHHLFSRPPIREKSRKQLQISSLKNDCSLFSRQHIASQIRLGDLDDFFQNKKQACLLSLSQIGCLRTETKSDLMPCLENLASVKEDLSTLRVQVNILDGAGIINMLQPGTAKTFQGYAADFLVSYVKSQLQHIDRQGIFWDLYIPDSLKADTRSKRKKEVRRRVEPSSAVSGNWKEFLRIEKIAWDTWTTYGDVTPAFCVLRAMPDPRATDEWMQPLEPFVALLYDRTSTEEDVNHARKRLYSKRGRAIDGLPST</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YSS7</accession>
    <name>A0A9Q0YSS7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Natterin-4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_32003</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YSS7"/>
    <dbReference type="OrthoDB" id="1925699at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.15.10">
        <property type="entry name" value="Proaerolysin, chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006616">
        <property type="entry name" value="DM9_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053237">
        <property type="entry name" value="Natterin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39244:SF5">
        <property type="entry name" value="NATTERIN-3-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39244">
        <property type="entry name" value="NATTERIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11901">
        <property type="entry name" value="DM9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56973">
        <property type="entry name" value="Aerolisin/ETX pore-forming domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040377528" evidence="2">
        <location>
            <begin position="28"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="362"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8027000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="387" mass="42163" checksum="1C578B45EC803284" modified="2023-09-13" version="1">MEIKEVSITKITLLTLVLIGSLKNVVGQEWVHTSGPPPPFPFSGSTVDTNDRTLFVCRGSVGGEMCSGKTWLSGGQLVSCKVPYGGREYSLSYFEVLTAPSWADYELTWSESTHSWGYLPDGAVSAGPGIYVGRYEQLFGGYLLGQVNYDELGFFYTEDGKEYSKKINYEVLTIMSRPIAQYEILNIVYDLSKATQTLSADDVILASKDVTNDTPYDTTSSLSMDITETKTSQWSETGSLEMHTGISVSVSAGIPYIGKLTGKWEAGLSSTYSYTYGESFTSSVTTSHEISVDLPQYSDTRVSIIAKTANVKVPYTATIRVVFSNGYTYVMDGVKGLYTDAHHTAFQTTVETSAKPSTTSGAATASLCGVMMFSTLFILWTGMLAFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YU65</accession>
    <name>A0A9Q0YU65_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Elongin-A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_31302</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YU65"/>
    <dbReference type="OrthoDB" id="21513at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070449">
        <property type="term" value="C:elongin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00183">
        <property type="entry name" value="TFIIS_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.10">
        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051870">
        <property type="entry name" value="Elongin-A_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010684">
        <property type="entry name" value="RNA_pol_II_trans_fac_SIII_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003617">
        <property type="entry name" value="TFIIS/CRSP70_N_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035441">
        <property type="entry name" value="TFIIS/LEDGF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017923">
        <property type="entry name" value="TFIIS_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15141">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR B POLYPEPTIDE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15141:SF76">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR B POLYPEPTIDE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06881">
        <property type="entry name" value="Elongin_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08711">
        <property type="entry name" value="Med26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00509">
        <property type="entry name" value="TFS2N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47676">
        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51319">
        <property type="entry name" value="TFIIS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TFIIS N-terminal" evidence="5">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="89"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="807"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="95"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="141"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="164"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="176"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="216"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="246"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="259"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="291"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="333"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="382"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="449"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="465"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="484"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="544"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="601"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="872"/>
            <end position="881"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8026476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="881" mass="99168" checksum="46EB74FE2C6468DD" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BAE9</accession>
    <name>A0A9Q1BAE9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">E3 SUMO-protein ligase NSE2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">E3 SUMO-protein transferase NSE2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Non-structural maintenance of chromosomes element 2 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">HOLleu_40294</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">Nanhai2018</strain>
            <tissue evidence="17">Muscle</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein sumoylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NSE2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BAE9"/>
    <dbReference type="OrthoDB" id="26899at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030915">
        <property type="term" value="C:Smc5-Smc6 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061665">
        <property type="term" value="F:SUMO ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16651">
        <property type="entry name" value="SPL-RING_NSE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026846">
        <property type="entry name" value="Nse2(Mms21)"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004181">
        <property type="entry name" value="Znf_MIZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21330">
        <property type="entry name" value="E3 SUMO-PROTEIN LIGASE NSE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21330:SF1">
        <property type="entry name" value="E3 SUMO-PROTEIN LIGASE NSE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11789">
        <property type="entry name" value="zf-Nse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51698">
        <property type="entry name" value="U_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51044">
        <property type="entry name" value="ZF_SP_RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="17" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SP-RING-type" evidence="15">
        <location>
            <begin position="148"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="U-box" evidence="16">
        <location>
            <begin position="156"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="52"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAJ8020646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="238" mass="26775" checksum="25EF316DE9B59B3A" modified="2023-09-13" version="1">MASGRAHFDVVDSAVRGLTKVQTYIEHGMNDIIEVSLDLAENGADEGSLHELETVMKEYVHLNNDLHQYNQAVKAVTQQMKQQDPKSDFDVDGELQCQLENIANGESQQNVENHAKMVELREKLWHLQHPGEEMGSQLVPVDAANNSDDDEPTMTQVEVSTRCPISQAEMADPVKNKICGHNYEKKAVAEIMRQRRGAKCPVTGCHNKQSLAQKDLVENRELKSYIERRNRQAGKKGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BAS2</accession>
    <name>A0A9Q1BAS2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_43412</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000297">
        <property type="protein sequence ID" value="KAJ8018543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BAS2"/>
    <dbReference type="OrthoDB" id="8961218at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="1">
        <location>
            <begin position="7"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8018543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="325" mass="36620" checksum="9CFD113C1430365A" modified="2023-09-13" version="1">MTLSSFVPGSAPCILAGDFNCVPDTQLDRMSIRTSSGCSVGMSELDLFIKNHDMVDVWRAQHPGLSVFTWHRPDGTDASRLDRVYSPSALSPSDCEVVTCQVPRQGGLGLVHFPSKLRFLLTKCVFSAVESQMPFAYFVRYWGGLSFRWFFPTLFSNSVPHSWRPTKAYSQMRETLRIMSAVGDVLQSSAGDQYRAIFDDLVGQVQPNDYASGPEVWRVVHSGTLDHRRRDLLWRIAHDAIVTNLKRLRWRLGDGICPRTGCTGWESVPHVFWQCGFVALLWEWFQALADRLTFCNTWAVSQDYALYGLSPPPCNTSAGLVERPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BDB6</accession>
    <name>A0A9Q1BDB6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Reverse transcriptase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_40773</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8021019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09274">
        <property type="entry name" value="RNase_HI_RT_Ty3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22249">
        <property type="entry name" value="UDM1_RNF168_RNF169-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000115">
        <property type="entry name" value="Polyprotein of retroviral origin, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000032">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 297-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon gypsy-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054465">
        <property type="entry name" value="Integrase_p58-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041373">
        <property type="entry name" value="RT_RNaseH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22938">
        <property type="entry name" value="Integrase_p58_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17917">
        <property type="entry name" value="RT_RNaseH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="9">
        <location>
            <begin position="886"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase" evidence="8">
        <location>
            <begin position="1300"/>
            <end position="1477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="96"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="352"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="619"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="719"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="352"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="367"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="394"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="619"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="643"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="663"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="688"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="735"/>
            <end position="760"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8021019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1692" mass="193978" checksum="E18FC8735E2DA47B" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BFF3</accession>
    <name>A0A9Q1BFF3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Apoptotic protease-activating factor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_33423</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BFF3"/>
    <dbReference type="OrthoDB" id="10031931at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070513">
        <property type="term" value="F:death domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002020">
        <property type="term" value="F:protease binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01671">
        <property type="entry name" value="CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001315">
        <property type="entry name" value="CARD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037939">
        <property type="entry name" value="CRADD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15034">
        <property type="entry name" value="DEATH DOMAIN-CONTAINING PROTEIN CRADD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15034:SF5">
        <property type="entry name" value="DEATH DOMAIN-CONTAINING PROTEIN CRADD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00619">
        <property type="entry name" value="CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00114">
        <property type="entry name" value="CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50209">
        <property type="entry name" value="CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CARD" evidence="2">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="122"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8025776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="180" mass="20072" checksum="3858D0C92DD2FF7C" modified="2023-09-13" version="1">MDPRDKWRLRSNRRTLLDIDASEVCGHLVSEGILTPDDSERIASEVTHKDKAESLLNLLETKGDKAFRAFRETLHKLSRPDLVKKLDETDMAKMPEALRKQLGGDATDKGNIIITTTETGKKRQVTIEDEGSASGRGGGRHQGQQDAKRSNVCNITVSGDNNVVLNDSKVVINSWHNKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BL39</accession>
    <name>A0A9Q1BL39_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Proto-oncogene c-Fos</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_30935</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BL39"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000837">
        <property type="entry name" value="AP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23351:SF24">
        <property type="entry name" value="ACTIVATING TRANSCRIPTION FACTOR 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23351">
        <property type="entry name" value="FOS TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00042">
        <property type="entry name" value="LEUZIPPRFOS"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="6">
        <location>
            <begin position="112"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="31"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="104"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="144"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="34"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="64"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8028637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="192" mass="21890" checksum="C10BB22E45A57B41" modified="2023-09-13" version="1">MITDPPHPMTGLLTPLDELPLFNGELEALLEENMSAREAETTAEEGKGATALAGSSTAQDLGGQEYISSGDESSSSDQISTDLQILQQKIQEGLAGMRWKPKFPQQLTPEGEEKRRVRRERNRQAASRCRDRRRERTFALVQESEKFEDDNQSLMSEIQELERQKRELEEWIVIHQQSGQCIKQIEEIHIDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BLQ1</accession>
    <name>A0A9Q1BLQ1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">N-acetylgalactosamine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_28208</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GHMP kinase family. GalK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8028946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BLQ1"/>
    <dbReference type="OrthoDB" id="187738at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004335">
        <property type="term" value="F:galactokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006012">
        <property type="term" value="P:galactose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000023">
        <property type="entry name" value="Putative N-acetylgalactosamine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000705">
        <property type="entry name" value="Galactokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019741">
        <property type="entry name" value="Galactokinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019539">
        <property type="entry name" value="GalKase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006203">
        <property type="entry name" value="GHMP_knse_ATP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006206">
        <property type="entry name" value="Mevalonate/galactokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10457:SF7">
        <property type="entry name" value="GALACTOKINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10457">
        <property type="entry name" value="MEVALONATE KINASE/GALACTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10509">
        <property type="entry name" value="GalKase_gal_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000530">
        <property type="entry name" value="Galactokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00473">
        <property type="entry name" value="GALCTOKINASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00959">
        <property type="entry name" value="MEVGALKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00106">
        <property type="entry name" value="GALACTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00627">
        <property type="entry name" value="GHMP_KINASES_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Galactokinase N-terminal" evidence="7">
        <location>
            <begin position="30"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP kinase N-terminal" evidence="6">
        <location>
            <begin position="114"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF10509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8028946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="395" mass="43533" checksum="94619346384D8C2D" modified="2023-09-13" version="1">MTEEEVYPNVLDIGAIPPAQTGRYKKIIAHHKEKYGCLPVFVARAPGRVNIIGEHIDYNGYSVLPMAIQQDIVMAVSLNDSGTINLCNTSSKYSDFSTSIKDFEINSTKPAWHNYFLCGVKGIQESSKLADTVGLDVTVDGSVPPSAGLSSSSAMVCCAALSTMHANKLKISKMELADTCMRCEWYIGTQGGGMDQAICFLAKEGYAKLVDFKPLRATDVKLPKGFVFVIANTCVEMNKAATAHFNVRVVECRLASQILAKTKGLDWRKNRSLGELHKHLKLSLEELLSLVDEVLHQHPYDKKEVCELLGVTEEELNKESLSSNTLDVQTFKLYNRSKHVFSEAARVESLRNSDMTEGGTLGSILFEGHHSASVLYECSCDELDELVEVCRCVCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BLX2</accession>
    <name>A0A9Q1BLX2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Leucine-rich repeat-containing G-protein coupled receptor 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_28407</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BLX2"/>
    <dbReference type="OrthoDB" id="1055097at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366">
        <property type="entry name" value="IG(IMMUNOGLOBULIN) AND LRR(LEUCINE RICH REPEAT) DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366:SF96">
        <property type="entry name" value="LEUCINE RICH REPEAT CONTAINING 53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0675">Receptor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="488"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="563"/>
            <end position="590"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8029092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="590" mass="67065" checksum="28F29795C1D7D305" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BMR3</accession>
    <name>A0A9Q1BMR3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transmembrane 6 superfamily member 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_28888</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BMR3"/>
    <dbReference type="OrthoDB" id="8181520at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14568:SF8">
        <property type="entry name" value="EXPERA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14568">
        <property type="entry name" value="TRANSMEMBRANE SUPERFAMILY 6 MEMBER 1/2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8029485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="127" mass="14609" checksum="80978BFEED507DE1" modified="2023-09-13" version="1">MITSTHIHVGPKFIPFLSYQAKTVQAKSIFKRPLDLLFVLYLCFAAVFSFLRVLGQITHIGSSVHSRTPYIYRIPSSVRPVVYLLNLLLLIVPQVMAWKFVYYPNYFANVVSNQESSTNGQLKKKQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BMV1</accession>
    <name>A0A9Q1BMV1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_28732</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BMV1"/>
    <dbReference type="OrthoDB" id="360161at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00268">
        <property type="entry name" value="DEADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23022">
        <property type="entry name" value="zf-HIT_DDX59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044742">
        <property type="entry name" value="DEAD/DEAH_RhlB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007529">
        <property type="entry name" value="Znf_HIT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF1">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04438">
        <property type="entry name" value="zf-HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 10" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="9">
        <location>
            <begin position="284"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="8">
        <location>
            <begin position="315"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="6">
        <location>
            <begin position="284"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8029359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="564" mass="62818" checksum="C28316B88D00FA3E" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BNL3</accession>
    <name>A0A9Q1BNL3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF4440 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_29532</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BNL3"/>
    <dbReference type="OrthoDB" id="5970825at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8029977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="88" mass="9703" checksum="B356E67F7B985CD1" modified="2023-09-13" version="1">MAPGSPMVTGREATAKMLQTDHESGFKTMKSVVEEVGNAGGNVIYSRGLYTFYTADGKEGDTGKYIALWRRVDGHLYLYTDIFCSDKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BPS6</accession>
    <name>A0A9Q1BPS6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">phospholipase A2</fullName>
            <ecNumber evidence="1">3.1.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_26929</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1-acyl-sn-glycero-3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15801"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.4"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:15802"/>
        </physiologicalReaction>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.4" evidence="1"/>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BPS6"/>
    <dbReference type="OrthoDB" id="10021675at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047499">
        <property type="term" value="F:calcium-independent phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052816">
        <property type="term" value="F:long-chain fatty acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000304">
        <property type="term" value="P:positive regulation of ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047148">
        <property type="entry name" value="PLPL9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24139:SF34">
        <property type="entry name" value="85_88 KDA CALCIUM-INDEPENDENT PHOSPHOLIPASE A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24139">
        <property type="entry name" value="CALCIUM-INDEPENDENT PHOSPHOLIPASE A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13857">
        <property type="entry name" value="Ank_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="151"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="322"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="355"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="PNPLA" evidence="9">
        <location>
            <begin position="489"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXGXXG" evidence="8">
        <location>
            <begin position="493"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DGA/G" evidence="8">
        <location>
            <begin position="660"/>
            <end position="662"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8030488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="816" mass="91477" checksum="4EDAF35D0295D4C7" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BQ36</accession>
    <name>A0A9Q1BQ36_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BRCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_27234</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="5997366at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16589">
        <property type="entry name" value="BRCT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRCT" evidence="2">
        <location>
            <begin position="1247"/>
            <end position="1346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="130"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="138"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="156"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8030742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1481" mass="168867" checksum="EAF96B92C55EBFA2" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BQI2</accession>
    <name>A0A9Q1BQI2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Alkaline ceramidase</fullName>
            <ecNumber evidence="9">3.5.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_27345</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the alkaline ceramidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.-" evidence="9"/>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BQI2"/>
    <dbReference type="OrthoDB" id="187171at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016811">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006672">
        <property type="term" value="P:ceramide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071602">
        <property type="term" value="P:phytosphingosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008901">
        <property type="entry name" value="ACER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46187">
        <property type="entry name" value="ALKALINE CERAMIDASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46187:SF3">
        <property type="entry name" value="ALKALINE CERAMIDASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05875">
        <property type="entry name" value="Ceramidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="36"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="69"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="92"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="122"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="145"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="175"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="222"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR608901-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR608901-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8030819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="283" mass="32559" checksum="626D060F176C32A9" modified="2023-09-13" version="1">MAPVEVNGMSGYWGKPTALLEWCEDNYEVTYYIAEFWNSFSSFMFIIPAFVGILFAIQDKLEVRFICSYSGMILVGIASFVFHMTLKFETQILDELSMVWAITFFVYSAYECNSPPKSTNYPLAAFVVLYSACVSISYVMLQQPVFHQAAFGLVTICIIARTFYCARRMTYSKFLLWYPLVMYVAAFTLWLIDNNFCDQLRAIRSHLPFPFSGFFQLHAWWHVFTALASQSQAAFNAHMRSNYLKQDFRVTGGPIPFVRLDHHGSRNGVTLPRQALNGNKKSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BQW8</accession>
    <name>A0A9Q1BQW8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">SCAN domain-containing protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_24595</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BQW8"/>
    <dbReference type="OrthoDB" id="6690226at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8031413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="59" mass="6819" checksum="16044A37213CAD8A" modified="2023-09-13" version="1">MAFPTSYLVERGFSTVMVLLTKYRNCLDITGRDDLRLFLTKMEPDIRNPTALHQAHPSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BR28</accession>
    <name>A0A9Q1BR28_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Lymphokine-activated killer T-cell-originated protein kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_27994</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8031307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BR28"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43289:SF14">
        <property type="entry name" value="LYMPHOKINE-ACTIVATED KILLER T-CELL-ORIGINATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43289">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 20-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 6" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="43"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8031307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="342" mass="38308" checksum="71AB977D13E21DDC" modified="2023-09-13" version="1">MSVKNKIPDDNFCTPTQPAKRSRISALNRSLKEDSPAIHIPASPFMERLGYGTGVSVYLFQRSPRQGDLLKSPWAVKKVKACPKFKGDIDTAKRLEKEAELLKKFKHEHIIGYRAFSRSADGTLCLSMENGEKSLMDLIEDRGEGDGDPFPAEDILKTALATAKALSYIHNEHKVLHGDLKSANILIKGDFESIKLCDFGVSVKLKEDLSDVKHANFLCLCWKDDNEFYIGTEPWSAKEVINGGKITDKTDIFAFGLIIWEMLALTIPHTHLLEDCVDSDGDGTLDESQYLESLGTRPPLPDFDYDDSYIPVLEILAMCTNEDPIERPSAREIVQILDTEKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-04-02" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BRE7</accession>
    <name>A0A9Q1BRE7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Remodeling and spacing factor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_27858</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8031204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10055895at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031213">
        <property type="term" value="C:RSF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15543">
        <property type="entry name" value="PHD_RSF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.1150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028938">
        <property type="entry name" value="Rsf1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14296">
        <property type="entry name" value="REMODELING AND SPACING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14296:SF16">
        <property type="entry name" value="REMODELING AND SPACING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="6">
        <location>
            <begin position="676"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="213"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="259"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="330"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="848"/>
            <end position="1216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1243"/>
            <end position="1271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="225"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="259"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="341"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="360"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="395"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="489"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="499"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="522"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="553"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="581"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="607"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="654"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="848"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="899"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="918"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="942"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="956"/>
            <end position="971"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1018"/>
            <end position="1030"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="1069"/>
            <end position="1078"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="1104"/>
            <end position="1116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1117"/>
            <end position="1126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="1161"/>
            <end position="1175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1176"/>
            <end position="1212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1247"/>
            <end position="1258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8031204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1286" mass="145900" checksum="8F1931052453ABED" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BS10</accession>
    <name>A0A9Q1BS10_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">FK506-binding protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_25036</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BS10"/>
    <dbReference type="OrthoDB" id="10573609at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.340">
        <property type="entry name" value="Nucleoplasmin core domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041232">
        <property type="entry name" value="NPL"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17800">
        <property type="entry name" value="NPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleoplasmin-like" evidence="1">
        <location>
            <begin position="2"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF17800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8031736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="181" mass="20277" checksum="723B88AEB790E3F9" modified="2023-09-13" version="1">MTLYPGVEYTREIKRPEYLTLAALDCRPQMNRGETNLTAYLVLKCDNYEHVLCTLVQGHAHQQILNFVMQEGEKMTLTVEGSGIVHVTGYTFKQLVLSDDTDTRTETTLMREVSDGIQDGEVLSPLPSSEGDDCTIIGMLTFNSEKFVKSNNPRSYFISKPTCKSEINICIVILTVCIKAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BU96</accession>
    <name>A0A9Q1BU96_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_22833</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8032783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BU96"/>
    <dbReference type="OrthoDB" id="7480422at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33332">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8032783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="235" mass="27394" checksum="C34CA1532331A351" modified="2023-09-13" version="1">MRFQPSKCNIITFARKKNPVKFDYVLNDVKLVKVDSIKYLGVTISNDLNWDKHIQSICNKGNKILGVLYRNLSFCSRDVKLAAYKGLLRPVLEYACCVWDPHQSYLQDKLESIQRRSARFISSEFSREPGSMTVILKDLDLPTLAERRKQNRLILFSKGFFGKANIPMDRLRHPTRTTRGMHNLHFCQLYSRSNCYKFSFFPKTLKDWNNLPADLIDGLDGHLDPVHYLTNFIRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BV12</accession>
    <name>A0A9Q1BV12_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Prostaglandin D2 receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_23443</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BV12"/>
    <dbReference type="OrthoDB" id="5959154at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007204">
        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008365">
        <property type="entry name" value="Prostanoid_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11866">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR FAMILY 1 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11866:SF16">
        <property type="entry name" value="PROSTAGLANDIN E2 RECEPTOR EP4 SUBTYPE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="23"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="68"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="105"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="147"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="200"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="268"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="39"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8033262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="309" mass="35147" checksum="ADCA3DEF04027E26" modified="2023-09-13" version="1">MEYTKATTPSMETLPPNEGGRLIFLYIVYCGITVSLTIGLIFHILAILATRKANKIKTEDNCHLLPTFLLNCLLRIDMLAVIFFFVRGFLPFIPSVRQHPIWCEFFVSTGIFFTWSSGLANCLMCAEKSISLIAPFFHRTYVTLRKAKVSLAILQLSALLICCLPFFGFGTYKIIEETTNVYDCVSPGEAQAIKANKYHLYFSIIFFVVGFSIIACILIGNCIVVYFIFHLKNKAAHLKITAIPAASQNADQNSSNSRMRKSSRETRFAKIMILVSISYTISWLPLYVSTSFFFDLFHFHPVLSHRYWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BW14</accession>
    <name>A0A9Q1BW14_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_21149</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BW14"/>
    <dbReference type="OrthoDB" id="6154697at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8034353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="105" mass="12169" checksum="7891298C5425BBF8" modified="2023-09-13" version="1">MTSILKDLDLPTLADRRKQNRLILFSKGFFGKANIPMDKISHPTRKTRGMHNLHFCQLYSRSNCYKFSFLPSTLKDWNNLPAALIDGLDSHRDPAQYITNFIRAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BWB1</accession>
    <name>A0A9Q1BWB1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_24483</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8034061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BWB1"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="323"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="339"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8034061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="364" mass="40770" checksum="A44F632B337FD0FE" modified="2023-09-13" version="1">MEIEIGGKKKRHVFWVADSGPNCILGLDFLRQHDCVIYASRDQVLIKDTRKDEIKPNNANDEIGADEVCCRIVASETILVPAQSEMLIFVTVKDFQETIAEEVKMMNPSNSLFGKKGALVATCVVDVGQGVVPVRLANLSDTPVTGYRDTTVGVCEGVGEVSSGSGGDVATKDLHKGTELFPEHLEKLFGTCMEELDNTQKDELEEKIRVTNAQARRNLKMAADRQSFRVNCKALEETFEKGDLVVVHSPAVKKGTTSKLHRPWQGPGLLLKRINDVLFRVTMGPQKNPKLIHYNWLRPYEGEELSWVKPFLEKSSNNAKKYSEEIRGPNPEDLCQRSCESEGKRGADRRGNERNHRDMVLVKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BX96</accession>
    <name>A0A9Q1BX96_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_21246</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BX96"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8034417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="64" mass="7294" checksum="4EA5F91051B1FC58" modified="2023-09-13" version="1">MESSREFNFNAAFLEFSRRNSLSLFARGTSSYATARCRSTTVPSSTCMHYQPLDQTRIPLRKGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-04-02" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BZG1</accession>
    <name>A0A9Q1BZG1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_20159</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="88467at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040116969" evidence="2">
        <location>
            <begin position="21"/>
            <end position="1534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="757"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="762"/>
            <end position="782"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8036243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1534" mass="169007" checksum="3E2143FF16E3ADA4" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BZI6</accession>
    <name>A0A9Q1BZI6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Multiple epidermal growth factor-like domains protein 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_19553</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8035777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BZI6"/>
    <dbReference type="OrthoDB" id="409374at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005044">
        <property type="term" value="F:scavenger receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00055">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.300.10:FF:000041">
        <property type="entry name" value="Tyrosine protein kinase receptor tie-1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.300.10">
        <property type="entry name" value="Tie2 ligand-binding domain superfamily"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002049">
        <property type="entry name" value="LE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042635">
        <property type="entry name" value="MEGF10/SREC1/2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24043:SF8">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24043">
        <property type="entry name" value="SCAVENGER RECEPTOR CLASS F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23106">
        <property type="entry name" value="EGF_Teneurin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00011">
        <property type="entry name" value="EGFLAMININ"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00180">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1 5" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 8" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040351283" evidence="8">
        <location>
            <begin position="24"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="365"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="9">
        <location>
            <begin position="236"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="395"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="406"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="260"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8035777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="481" mass="52730" checksum="7593F84163682506" modified="2023-09-13" version="1">MSHIPNAVLLLVLFSCWICGTISSAISEDEDHICRKTRLVKLLDHVGQQDDWSCENYFPFQSCKKIRSGDVNFDEVFIPAEELICCPGWMRDDLGFCRLACPEGLYGEDCRQPCHCVHGHNGQNCDPVDGQCECQEGWTGRDCNERCPPGSFGKNCNESTLPCLNEGINDPLTGECLCPVTHQGEFCGEQCPSCVVECHNCADSHTEVCDTVIAACRCKAGWTGYFCTEPCPSGSGGMFCAEECCSGNGLCLDGSKSCRCNDGYRGENCEEKCDEGTFGPDCSQKCLWPHCDHVTGQCHQCPIGYTGESCTSICSEGRWGPGCNESCHCEADELCDIFTGTCSEVKITDYSRGDEPQPSVTITGALTAAGGLVLLVLVAIIMYVSIKCLKRKDTQSTPNEAFPSLNIKSDTSSNKRVNDSNGELRKDDYSSVYQEIDDYPSRYTKPALPSAPPGIPLYKDQPSYERVLNQFDPDYLVPDVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C098</accession>
    <name>A0A9Q1C098_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Apple domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_19841</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8035992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C098"/>
    <dbReference type="OrthoDB" id="5775605at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.4.10">
        <property type="entry name" value="Hepatocyte Growth Factor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052774">
        <property type="entry name" value="Celegans_DevNeuronal_Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47327">
        <property type="entry name" value="FI18240P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47327:SF7">
        <property type="entry name" value="GH08941P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00024">
        <property type="entry name" value="PAN_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00473">
        <property type="entry name" value="PAN_AP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57414">
        <property type="entry name" value="Hairpin loop containing domain-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50948">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Apple" evidence="1">
        <location>
            <begin position="21"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8035992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="365" mass="41962" checksum="D49BE45A350C58D6" modified="2023-09-13" version="1">MKTILYFINFAMYIAVRQQRCSALNGEWTSVVSACIRGHNNVVTHSTSVQACQELCEQQDNFRCLSIDFHSNGGNCHLSRYNRNSVPSTDDFTQPCYLDGWLYAERKDLDFPWSEVVHGCIRGNNRRDYSTSSMEACQLRCERETSFPCLSVDFKDGQCLLSDFNRESVRPLSDYTQPCYIEGFQYSERTDKKYFWTETASACIRGNNNEHHNNINSLEACKDLCYQENTFACLSVDYHPTHANKPCHLSSFNRNSVQPSGDFTQPCYLDGWLYAERVNVGQWIPVIERACIRGHNEWEADTEDFESCKILCSQKSNCCSIDYNRETKRCLMTSHTRASVTDSTFLSPCSGYSYSERTDLRCVTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C0G0</accession>
    <name>A0A9Q1C0G0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Serine protease inhibitor dipetalogastin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_20246</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C0G0"/>
    <dbReference type="OrthoDB" id="126772at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="KAZAL_FS"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR036058">
        <property type="entry name" value="Kazal_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050653">
        <property type="entry name" value="Prot_Inhib_GrowthFact_Antg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10913:SF45">
        <property type="entry name" value="FOLLISTATIN, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10913">
        <property type="entry name" value="FOLLISTATIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00050">
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        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07648">
        <property type="entry name" value="Kazal_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00280">
        <property type="entry name" value="KAZAL"/>
        <property type="match status" value="18"/>
    </dbReference>
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        <property type="entry name" value="Kazal-type serine protease inhibitors"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51465">
        <property type="entry name" value="KAZAL_2"/>
        <property type="match status" value="18"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0722">Serine protease inhibitor</keyword>
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        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040191881" evidence="4">
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            <begin position="18"/>
            <end position="1193"/>
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    <feature type="domain" description="Kazal-like" evidence="5">
        <location>
            <begin position="149"/>
            <end position="207"/>
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    <feature type="domain" description="Kazal-like" evidence="5">
        <location>
            <begin position="208"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="Kazal-like" evidence="5">
        <location>
            <begin position="256"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="Kazal-like" evidence="5">
        <location>
            <begin position="306"/>
            <end position="353"/>
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    <feature type="domain" description="Kazal-like" evidence="5">
        <location>
            <begin position="354"/>
            <end position="399"/>
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    <feature type="domain" description="Kazal-like" evidence="5">
        <location>
            <begin position="400"/>
            <end position="447"/>
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    </feature>
    <feature type="domain" description="Kazal-like" evidence="5">
        <location>
            <begin position="448"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="domain" description="Kazal-like" evidence="5">
        <location>
            <begin position="498"/>
            <end position="543"/>
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    </feature>
    <feature type="domain" description="Kazal-like" evidence="5">
        <location>
            <begin position="544"/>
            <end position="591"/>
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    <feature type="domain" description="Kazal-like" evidence="5">
        <location>
            <begin position="592"/>
            <end position="640"/>
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        <location>
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        <location>
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        <location>
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        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8036311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1193" mass="129964" checksum="556C139A0F206374" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C0S0</accession>
    <name>A0A9Q1C0S0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Succinate dehydrogenase assembly factor 3</fullName>
            <shortName evidence="6">SDH assembly factor 3</shortName>
            <shortName evidence="6">SDHAF3</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_20045</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Plays an essential role in the assembly of succinate dehydrogenase (SDH), an enzyme complex (also referred to as respiratory complex II) that is a component of both the tricarboxylic acid (TCA) cycle and the mitochondrial electron transport chain, and which couples the oxidation of succinate to fumarate with the reduction of ubiquinone (coenzyme Q) to ubiquinol. Promotes maturation of the iron-sulfur protein subunit of the SDH catalytic dimer, protecting it from the deleterious effects of oxidants. May act together with SDHAF1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with the iron-sulfur protein subunit within the SDH catalytic dimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the complex I LYR family. SDHAF3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C0S0"/>
    <dbReference type="OrthoDB" id="278329at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
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        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034553">
        <property type="term" value="P:mitochondrial respiratory chain complex II assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="P:succinate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="5 6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4 6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
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            <end position="118"/>
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    </feature>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU368039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8036158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="118" mass="13588" checksum="971C8CFB4CBAA1FC" modified="2023-09-13" version="1">MAAAVQARSVRALYRRILTLHKRLPIEMKAIGDEYAKAEFRRHKKVGPEEAANFVREWTVNYADMIEIQTSRDQPSSDIGAPLNHDSLEDFREEQLGQLLELHKETSKPSTFPHEDNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C135</accession>
    <name>A0A9Q1C135_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Adhesion G-protein coupled receptor G2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_20393</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8036427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C135"/>
    <dbReference type="OrthoDB" id="1100386at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053066">
        <property type="entry name" value="ADGR_G7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47767">
        <property type="entry name" value="ADHESION G PROTEIN-COUPLED RECEPTOR G7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="53"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="77"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="120"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="120"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8036427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="312" mass="35019" checksum="FB966AAC3E31F8F7" modified="2023-09-13" version="1">MVFYISVVGVVGLLITYNFIIFVLVTRKLTCARTTVTKANLSKQETFRRLQNVVAISFLLGVTWIFGLLSFGKLREVSNFIFCALNSFQGLCIFVLFCARQKEIRSAWKTWLLLSVGRSHQTGSRSSTSGRGLSKRKLISEESDEKTSSAIKSSSNSKVELSDLKSDGFEDEISHNIYSKVKETVNDGVEITDSACYSDIDPKDRNLPPIPEEGLYSKEIKSTVEQNGACGTTTVTPELYMEIGEGQTFLSEGNGYQSLTNERVDTQEYMDLSFGTSAHNSAELTRRSLVYEAQIQRHHPIAPRWQRHTINH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C2V5</accession>
    <name>A0A9Q1C2V5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Clusterin-associated protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_18175</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CLUAP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C2V5"/>
    <dbReference type="OrthoDB" id="438545at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030992">
        <property type="term" value="C:intraciliary transport particle B"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005815">
        <property type="term" value="C:microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019366">
        <property type="entry name" value="Clusterin-associated_protein-1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21547">
        <property type="entry name" value="CLUSTERIN ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21547:SF0">
        <property type="entry name" value="CLUSTERIN-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10234">
        <property type="entry name" value="Cluap1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0969">Cilium</keyword>
    <keyword evidence="3" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="4 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="311"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="191"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="323"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="389"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8037372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="431" mass="48940" checksum="3BCC384401B56C4A" modified="2023-09-13" version="1">MSYRDLRNFTEMMRALGYPRLISMENFRTPNFSLVAEVLVWLVQRYDPNADIPTDTDTEQDRVIFIKSVAQFMATKAHIKLNTKRLYQADGYAVKELIKVTSVLYNAMKTNTTNKDGEEDANNGSTSLSFDLSSKISDLKASRQLASLITSKGASLYDLLGKEVELREIRTTAINRPLEINEIENGLRGSVAAVEQNIDKTQKMLENVASDEANLEAKIDKKKSELERNQKRLQTLQSVRPAFMDEYEKLEAELAKCYEQFIEKYRNLTSLEHQLEDINRAEQDRFEETENSLKKMQTRLKEEEDRLKMTDDILGDDISGLDVVDDDGDDDDDSGSDEEEEQEEVKPTKGKFRQQAKDSQRNPRVFGSMGADISDDDESGSSVEGDSISVDDDDDEDDFLYEPADGEDDLEVGGARNRKEPEGDLDSDNDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C3H3</accession>
    <name>A0A9Q1C3H3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_18628</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C3H3"/>
    <dbReference type="OrthoDB" id="5586934at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33802">
        <property type="entry name" value="SI:CH211-161H7.5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33802:SF1">
        <property type="entry name" value="XK-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040448144" evidence="2">
        <location>
            <begin position="23"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="124"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="174"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="201"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="259"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8037735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="336" mass="38266" checksum="9E141E9B631033AD" modified="2023-09-13" version="1">MELDPFHLFLIILALLFTLCSAVNCCLAYFSGNASNSWFNHNRQDIANDPEYDTEITPANWVHGIWAVIFIWLVLWLVWHLMTICKRRKDLPHGPVSDILLAEYNLALSLLNVSYFAYDRKKFVGNLMALLLTSAIIIICLTIHIRNALQLRREFFQTYRTNFIWNHVLTQNGLTLFATWTGLEVLPSLAVILISQSDANQNVACGLSLAVFAFMGCVIIIIEYGRYAEHLNYIFTHWCVIIWGLSGSVAGNSDHTSSGVFITLCLFLGLSVLMFIMKVILVIQRYTNIRVPARQATVAHKYPSIQTMRGNNKNVKLRSGLHQTFSQIIDDDDILY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C5C8</accession>
    <name>A0A9Q1C5C8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Tripartite motif-containing protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_15885</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C5C8"/>
    <dbReference type="OrthoDB" id="265776at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017868">
        <property type="entry name" value="Filamin/ABP280_repeat-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050952">
        <property type="entry name" value="TRIM-NHL_E3_ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24104:SF57">
        <property type="entry name" value="BEE-MILK PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24104">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE NHLRC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101898">
        <property type="entry name" value="NHL repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50194">
        <property type="entry name" value="FILAMIN_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="7">
        <location>
            <begin position="15"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="5">
        <location>
            <begin position="142"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8038454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="552" mass="61247" checksum="CACB3EE061940300" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C5N9</accession>
    <name>A0A9Q1C5N9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_16769</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C5N9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8039146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="64" mass="7170" checksum="52D5F90655DE7BB7" modified="2023-09-13" version="1">MESSREFNFNAAFLEFSRSLALSLFARGTSSYATARCRSTTVPSSTCMHYQPLDQTRQILSKGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C5X6</accession>
    <name>A0A9Q1C5X6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_16876</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
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            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
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    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
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            <taxon>Aspidochirotida</taxon>
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        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
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            <strain evidence="4">Nanhai2018</strain>
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    </reference>
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        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22639:SF3">
        <property type="entry name" value="ZINC FINGER CCHC DOMAIN-CONTAINING PROTEIN 3"/>
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    </dbReference>
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        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
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    </dbReference>
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            <begin position="287"/>
            <end position="429"/>
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            <begin position="314"/>
            <end position="332"/>
        </location>
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            <begin position="416"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAJ8039515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="429" mass="47281" checksum="A5DFFB4C5DE56421" modified="2023-09-13" version="1">MDSSNPEKLGKKRFRSDSGASSDPEEAPIWHRFLVVSSSGDDKTLAKLSPFAISKGIEGVVGTPKTIKRLRSGDLLVEVSRSTQANNLLKTKKLVDVPVQVTPHRSLNSSKGVIRCPDIKNCSDEEIHDNLASQHVSHLYRISVLREGVRKPTGTFILTVNTPKPPTTLKIGYLQVRVEVYIPNPVRCFNCQRYGHFKTNCSRAATCEKCGQEGHAGDTCEESPRCVNCQGCHSDNDRTCPKWVEEKQIQKIKASSNITYKEARDTFLSQDSQLKSYAGAVRTAKVSTATQTDMTWPRDSSFPKPITLSFAKPTEQSTTSKTIQTMTAPTNLPQKSTPPQKKPKPPPKPTTTKQRQKTEPTKARDTPRIKIQTKPSKGSDDPIKLHNRFGALEGSEGEGEELMELSGRKSRPEPGGSTSSTNKPQISKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
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        <name evidence="3" type="ORF">HOLleu_15203</name>
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    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
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        <dbReference type="NCBI Taxonomy" id="206669"/>
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    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
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        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
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    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:negative regulation of calcium ion-dependent exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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            <begin position="16"/>
            <end position="41"/>
        </location>
    </feature>
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            <begin position="1153"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1205" mass="137195" checksum="7AC1EB070720D945" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CBQ3</accession>
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            <taxon>Eukaryota</taxon>
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            <authorList>
                <person name="Chen T."/>
            </authorList>
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            <strain evidence="1">Nanhai2018</strain>
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    </reference>
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        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8042010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="10377879at2759"/>
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        <property type="component" value="Chromosome 5"/>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
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            <dbReference type="EMBL" id="KAJ8042010.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
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    </evidence>
    <sequence length="217" mass="24653" checksum="5DB154E713F1DC8B" modified="2023-09-13" version="1">MSYVGKMDSLMNCRHSDGSLAWQYHINTHEVVFTTVLYATNVRPLDISVHQLPESIAYVQTNIELVWTISRTAILNFVIASQAAYEPKMIEMVGRVIVEFDFAVVEPYILEDGTLDTSRIHLEMGISTYTPVSNSSLLVFMGKDFLYYIADTPGNVMTEVLKMVRYSNLPSLKGQGVDFKTYCSTYIKRVSCRPAYKIPQDDIQMSNRTSYIVATRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CCF2</accession>
    <name>A0A9Q1CCF2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF5641 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_13381</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8042351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CCF2"/>
    <dbReference type="OrthoDB" id="6377381at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040676">
        <property type="entry name" value="DUF5641"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331:SF1">
        <property type="entry name" value="GAG-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18701">
        <property type="entry name" value="DUF5641"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF5641" evidence="1">
        <location>
            <begin position="73"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8042351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="194" mass="22490" checksum="40F875661A6858B8" modified="2023-09-13" version="1">MLTRSELETTLREVEACINSRPLTYVGTDPDGPVPLTPSHFLLGRSFNSQPEVEERVVTSRDLAEKSLSWDHYSDIFWEKWRCDYLRNLPPAIKKFRSKSNLEVGSVVLVNEDNVPRMKWPLAVVVELYRGRDNLVRSVKLRTAKGIISRPFQRLYCLEMIMGENSDMEPTQLNEDKVYITRFGRKVVSRNLTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CCM8</accession>
    <name>A0A9Q1CCM8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Zinc finger CCHC domain-containing protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_10149</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CCM8"/>
    <dbReference type="OrthoDB" id="5987933at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002218">
        <property type="term" value="P:activation of innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042509">
        <property type="entry name" value="ZCCHC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22639">
        <property type="entry name" value="GAG-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22639:SF3">
        <property type="entry name" value="ZINC FINGER CCHC DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="2">
        <location>
            <begin position="122"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8043173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="163" mass="17840" checksum="22493D9D901F17AD" modified="2023-09-13" version="1">MLQALAARSTYDLGFGSDAARVKGVSCLKGVEGLTVTPYDRSVAVTVLYLSFEVDRRLVARVLSRCGTVSDMRWCKYGSGELAGIFNGKRQCRMELDRDIPSFLFIGGSKAHIRYFGQPRTCFKCGQEGHEARSCPNRRCGRCLQVGHDKAECPNECGCGAGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CCW3</accession>
    <name>A0A9Q1CCW3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methyltransferase-like protein 27</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_13380</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8042350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CCW3"/>
    <dbReference type="OrthoDB" id="3647at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591:SF110">
        <property type="entry name" value="RHODANESE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="1">
        <location>
            <begin position="138"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8042350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="299" mass="32901" checksum="6E2952D742B9773E" modified="2023-09-13" version="1">MGKRSGIIEMANMAGMQSNTPGVTEIFELMKNDHCNTAGSQVVRDLYDKCSSTYEKRSGIIEMANMAGMQSNTPGVTEIFELMKNDHCNTAGSQVVRDLYDKCSSTYEKETADLAFTGPNQTGIILSDHCPDRNISIVDCGAGSGLVGVQLNTLGYRNVVGVDISQKILEIAEQKGVYKNLFCVTVGEERMPFGDNEFDALVCCGCIAPAHISPSCFPEWVRIVRPGGSIVIVLRRCYVELQKDEEEFYSQSLGESFEANIRHLEDSKKWKAISKKIFSGYLTENGHANDGIGMVFEVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CDK8</accession>
    <name>A0A9Q1CDK8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_09355</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8042574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CDK8"/>
    <dbReference type="OrthoDB" id="10616474at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007157">
        <property type="term" value="P:heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051427">
        <property type="entry name" value="Nectin/Nectin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23277:SF108">
        <property type="entry name" value="FASCICLIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23277">
        <property type="entry name" value="NECTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="361"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="138"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8042574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="395" mass="43555" checksum="2A9D12748B1B9BB5" modified="2023-09-13" version="1">MQIVLRVASLGYILTAICPEGLHGKCTSPQYGEIGQEGIIKCVLPSSFNGVIWYDSEDTTTTSQIVLVERQSDGSFRTSGQGFDSGEYRISSNGSLIIPIVSIHHDRNFTVFSLDSNDEVFQAMVEFKTVVRPSMSVPAVNSCEEDNLCLINFNMVKTDTLTCTLSQARPAVVVSWHHQIEGNLENMFAQNNVKSDGQNTFTSTSKVNVSELINTSVRVFSCNASGPALSHGKYETIVVVYNIDIESINSHQHNILQKIVVINRSLEIPCKDHGTPMIFVWSFKGLSTSSVLAFSAQGEKRLIGNVNHTFDVLQDGTIIFEHVDYQLEGIFICTSIERGESSTFMLRVKVVGHEAEQIGKVLNICVGIFLALVFLAIASGGVWYVRKRKVSIINT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CEW5</accession>
    <name>A0A9Q1CEW5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_10253</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CEW5"/>
    <dbReference type="OrthoDB" id="6151331at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8043249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="78" mass="8924" checksum="DAED7D53E4C8EE90" modified="2023-09-13" version="1">MTSVTGYGPRYSLIFDGNERKYELWEIKFSGSMRLQQLHDVIVQPEGENGDVPVDRAKSAEAFAELVQFLDENGRCQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CF71</accession>
    <name>A0A9Q1CF71_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Mitochondrial import receptor subunit TOM20-like B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_07036</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Tom20 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CF71"/>
    <dbReference type="OrthoDB" id="2154253at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005742">
        <property type="term" value="C:mitochondrial outer membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030943">
        <property type="term" value="F:mitochondrion targeting sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016031">
        <property type="term" value="P:tRNA import into mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.960.10:FF:000001">
        <property type="entry name" value="Mitochondrial import receptor subunit TOM20 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.960.10">
        <property type="entry name" value="Mitochondrial outer membrane translocase complex, subunit Tom20 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002056">
        <property type="entry name" value="MAS20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022422">
        <property type="entry name" value="MAS20_rcpt_metazoan"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023392">
        <property type="entry name" value="Tom20_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12430">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12430:SF0">
        <property type="entry name" value="TRANSLOCASE OF OUTER MITOCHONDRIAL MEMBRANE 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02064">
        <property type="entry name" value="MAS20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01989">
        <property type="entry name" value="EUOM20RECPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00351">
        <property type="entry name" value="OM20RECEPTOR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47157">
        <property type="entry name" value="Mitochondrial import receptor subunit Tom20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="103"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8044313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="130" mass="14268" checksum="54BF209B404A92C7" modified="2023-09-13" version="1">MDHIKRRAERERQKKQSGSGTDIKLPNLNDPEAVQKFFLEQVQIGEELLAQGSFEEGVEHLSNAVAVCAQPQQLLQLLHQTLPKQVFQMLVKKLPATSHRIASMSAKSGSAAPPPFGAPTATLAEEDDLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CFD3</accession>
    <name>A0A9Q1CFD3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_10910</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CFD3"/>
    <dbReference type="OrthoDB" id="5421239at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009976">
        <property type="term" value="F:tocopherol cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025893">
        <property type="entry name" value="Tocopherol_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35309">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35309:SF4">
        <property type="entry name" value="TOCOPHEROL CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14249">
        <property type="entry name" value="Tocopherol_cycl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8043703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="237" mass="27170" checksum="9F9FAFA9BC51319D" modified="2023-09-13" version="1">MFDLRERLESPYRGQRVDLGPEGWLHVLPIPLHWFVYSLASPGFYRWINLTSNEILEGEGFLHQEKNWGKSFPPEWVWTEGYDPFQNITYAGTFGTLNFGPFLVPAMLFGYRNYKRGLSIDFRPDNSFVTKDLNPCKGLASVTIYSVRYTVYVNISAPQSTFETCLCGPNDYGFYPLVTESFVSTTRIQVKEHLTWWDSVYRSGAIIEDAVIQRVALEFGGAFMCDRNPCSKKTTCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CFM9</accession>
    <name>A0A9Q1CFM9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_10803</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CFM9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8043624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="62" mass="6738" checksum="36A6A171E82BC399" modified="2023-09-13" version="1">MNCTPSPQPPPASPSASSQPPPSLRTYTVPKGCLTMNTWKCVEIVVITFLSITNNQYQTEIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CH39</accession>
    <name>A0A9Q1CH39_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">3-hydroxyacyl-CoA dehydrogenase F54C8.1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_08133</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CH39"/>
    <dbReference type="OrthoDB" id="2021159at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006176">
        <property type="entry name" value="3-OHacyl-CoA_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48075">
        <property type="entry name" value="3-HYDROXYACYL-COA DEHYDROGENASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48075:SF5">
        <property type="entry name" value="3-HYDROXYBUTYRYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02737">
        <property type="entry name" value="3HCDH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="3-hydroxyacyl-CoA dehydrogenase NAD binding" evidence="4">
        <location>
            <begin position="3"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8045183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="364" mass="40454" checksum="058C07511D43BE91" modified="2023-09-13" version="1">MVKIAVCGCGFLGSSIAGCLIFFGHDVIIYDINRSALDRFPEELRKKKEELLERELILDRIFSGTYRLEAELEAAVKDVDFIIEAIQENVAAKKAFFQRVSNVCQPSTVLLTSSINFRLEDIFALTANKERTMGLRFLYPVYFVRDVEVQYHEKTSDEAKEKGGFRGVDKLGKTIFLRISGQDPLVLPQEDIIAMEEARRIKLARIRREMPSSTLSSSLQGERGSFATLGSNGHSEDFTDNVFGQENTRQAFHCHGASSSPAFRGAPNCPVFHGASSGQTFQQTACNNVSIPLSLRSLGKLALSLRSLDTETCLGCHLRPTMCVLSPCLHHMCKECAKSLMNRKKGCPVQGCSRDVNSYQTFGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CJ64</accession>
    <name>A0A9Q1CJ64_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">UDP-D-xylose:L-fucose alpha-1,3-D-xylosyltransferase 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_04965</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 77 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CJ64"/>
    <dbReference type="OrthoDB" id="197884at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005069">
        <property type="entry name" value="Nucl-diP-sugar_transferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052636">
        <property type="entry name" value="UDP-D-xylose:L-fucose_XylT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47032">
        <property type="entry name" value="UDP-D-XYLOSE:L-FUCOSE ALPHA-1,3-D-XYLOSYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47032:SF1">
        <property type="entry name" value="UDP-D-XYLOSE:L-FUCOSE ALPHA-1,3-D-XYLOSYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03407">
        <property type="entry name" value="Nucleotid_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleotide-diphospho-sugar transferase" evidence="3">
        <location>
            <begin position="109"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="34"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="34"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8046327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="336" mass="38788" checksum="FCAF537DE775360A" modified="2023-09-13" version="1">METVFQTFASGRTTAVCNFSDILHQSQFYHKRKQRYGHARQSHRNNEDEKRPSLSAVKPQEINKKDPKPPTLSDVASSLQSPVVLSAINEAFIEITENFILSIRKLNLTPSILIVCEDKRSYEVLSTWKNITVVMTHLQLSKSAAEDWRSKAYNKLVDKRVPYILELLQHGKDVFFMDTDIVWLADPFPYVHGKVDVAVIHDYPVNKEKKVIYCAGYAYFRNCPAAKFVVNHWQKMLVSPKRKEGSEQQGKFNKALKKGLGQKGNKKLRLKVLPRNLFQAGFEFFNSSTPLDERPVRPVMIHNNWIVGLAPKLERYKQFGLWFVESNRSSSETSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CJI3</accession>
    <name>A0A9Q1CJI3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_08792</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CJI3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8045729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="59" mass="6830" checksum="8D1DB9319ED6C875" modified="2023-09-13" version="1">MGADRIVYGMLVPWTPLQQCRLIQSNKRSIIVFRFYDLCYQSQSEVCYDQGPNHVGCNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CJK0</accession>
    <name>A0A9Q1CJK0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Organic solute transporter subunit alpha</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_09042</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CJK0"/>
    <dbReference type="OrthoDB" id="5832279at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005178">
        <property type="entry name" value="Ostalpha/TMEM184C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23423">
        <property type="entry name" value="ORGANIC SOLUTE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03619">
        <property type="entry name" value="Solute_trans_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01417">
        <property type="entry name" value="Solute_trans_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="33"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="159"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="197"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="235"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="277"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="338"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="344"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8045923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="359" mass="40568" checksum="8C8841010767968A" modified="2023-09-13" version="1">MSVLNFTNFCNGTTPDTKELYKVLDDIDGFKGVLIFSGIITPIVLCFFAEAVVYTVRNIPYSDRRYCILWILGVYPIFCVTALLGLFIPRAAGLCTFTAAAYFAVVLFEFMHLIVDYFGGLEAMLLQMKGTKMSVARPPTLCLFFFLPKFEMTRRNFRILEIGVLQVAFLRPLTLFITELLSIDGRVGPTSENARTISLTINSIILATSVTAIQCLLIFFYAARKYLSKFYIGPKFLCVQIALILSNVQSALLSILSRYNVIECYPPFPFIVRGYQWHLFLVTIESSLMLIPTLIFFRTVKGNILLDPHRKLRRTSTFAGLESSAKLRRRRSSTTVEGDRLLKSRNSQSGSSYGTVIEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CKU0</accession>
    <name>A0A9Q1CKU0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">DNA transposase THAP9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_05511</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CKU0"/>
    <dbReference type="OrthoDB" id="6491412at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021896">
        <property type="entry name" value="THAP9-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006612">
        <property type="entry name" value="THAP_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048366">
        <property type="entry name" value="TNP-like_GBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048367">
        <property type="entry name" value="TNP-like_RNaseH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048365">
        <property type="entry name" value="TNP-like_RNaseH_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47577:SF2">
        <property type="entry name" value="THAP DOMAIN CONTAINING 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47577">
        <property type="entry name" value="THAP DOMAIN-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05485">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21788">
        <property type="entry name" value="TNP-like_GBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21789">
        <property type="entry name" value="TNP-like_RNaseH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21787">
        <property type="entry name" value="TNP-like_RNaseH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12017">
        <property type="entry name" value="Tnp_P_element"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00692">
        <property type="entry name" value="DM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00980">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50950">
        <property type="entry name" value="ZF_THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="THAP-type" evidence="7">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="122"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8046735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="878" mass="99237" checksum="0708B93559262EC3" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CKW1</accession>
    <name>A0A9Q1CKW1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_06024</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CKW1"/>
    <dbReference type="OrthoDB" id="6361178at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8047118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="68" mass="7360" checksum="F6277D0057634829" modified="2023-09-13" version="1">MPHIERKNPIVFGGGQRSPGVTGGQNMKTLLTQYLKMRYGKRKNPIIFGGGQRSSGVTGGQTLKTLLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CLP8</accession>
    <name>A0A9Q1CLP8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_05625</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CLP8"/>
    <dbReference type="OrthoDB" id="5982932at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8046824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="192" mass="21669" checksum="C563248DF7E5CC8D" modified="2023-09-13" version="1">MDTIIYARRSLLFDESGNPWVKRDTQKEFDVTMGAYDGAEIAELVGLYILQTIKTELTNINAGLYSDDGLAVIKGTGRTVDKVRKDITKIFQSLGLKITVQANLKNVDFLDVNFDLPTGLHKPHRKRNDEPLYIDTGSNHPPEILKHIHPSIEKRISALFSNEEIFEKAAPIYNQALRNSGHNGKITYEKNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CNR2</accession>
    <name>A0A9Q1CNR2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_00194</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CNR2"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8048035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="119" mass="13694" checksum="8EC49F50CBDF2347" modified="2023-09-13" version="1">MLFHPWRNETGLKGTFETFQQHYKLLQTSLKEKEAGYNHNSEAVDQAIESLDTEDYDFIHGDIAPNTGHKEQLDTKEMEMANKKKSSEPHELYDIGQDMGIAVNNGNMEELQKPRLPET</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CS33</accession>
    <name>A0A9Q1CS33_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">RNA-directed DNA polymerase from mobile element jockey</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_03805</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CS33"/>
    <dbReference type="OrthoDB" id="411173at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016706">
        <property type="term" value="F:2-oxoglutarate-dependent dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015095">
        <property type="entry name" value="AlkB_hom8_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47510:SF3">
        <property type="entry name" value="ENDO_EXONUCLEASE_PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47510">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09004">
        <property type="entry name" value="ALKBH8_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="119"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8050557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="463" mass="52504" checksum="308B75A578AFEE18" modified="2023-09-13" version="1">MDNVLPDSEQYIDIPTRGATTLDLCYGNIANAYTSKSGHQLGSSDHLNIQLVPAYKQKLKRAPVVTKQRYDWSDDATERLTACLECMDWGVFTESVDTLDEATEVITGYINFCVDCNIPCKAIKVYPKLWKTSFNVPLPKVKSPVELNDFRSVALTCSVMKCFEKVLLSQLVKQTNIHLDPMQFAYRENGVEDAVLLYLQKALSYLDTAKTYVRSTFIDFSSAFNIHVPHFLIHKLEHMNVNPHILLVIHDFLLGKPQRVKMGQTISDIRTSSTGVPQGCVLSPVLFSLYTSECVSKDAECSVIKYANDTVITGYLSGDKGTDRYFSVIDTFVEWCDSHFLQLNVMQTKEIIMDFRKNKANGHMPVVIHGSVVEQVPEYKYLGTRITNNLDWSSNTIAAQKTANQLMFFIRQLKKIHLDNTLLVLFHKSIIQSILTFNLICCYGNLTKANRPKLIALVRLLRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CSB6</accession>
    <name>A0A9Q1CSB6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_03718</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
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    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CSB6"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101898">
        <property type="entry name" value="NHL repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8050493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="158" mass="17777" checksum="E4B496C21584520E" modified="2023-09-13" version="1">MTVRGHNVIVCDDEVGKAVALDMNGNVVYEFMKPADDSGNWIPIKVCCDSRGFVYILWCSGDNRRRVIVQNSLDSENVLRVLPVDDNAFSVTVMKASNKERLLVATVNTGKLAIYDLVSFRTRQIATRIPIKIVNIENENIVQYNFQYVGANVTQESF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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    <name>A0A9Q1CT50_HOLLE</name>
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        <recommendedName>
            <fullName evidence="2">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="3" type="ORF">HOLleu_04332</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
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            <taxon>Metazoa</taxon>
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            <taxon>Holothuroidea</taxon>
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            <taxon>Holothuriidae</taxon>
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        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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            <begin position="467"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
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            <begin position="11"/>
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        </location>
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    </evidence>
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        <source>
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    </evidence>
    <sequence length="727" mass="82811" checksum="78C0D5257A99DD55" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CU69</accession>
    <name>A0A9Q1CU69_HOLLE</name>
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        </recommendedName>
    </protein>
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        <name evidence="3" type="ORF">HOLleu_04474</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
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            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
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        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8051050.1"/>
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            <begin position="193"/>
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        </location>
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    </evidence>
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        </source>
    </evidence>
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            <dbReference type="EMBL" id="KAJ8051050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="329" mass="35935" checksum="40929E497C2840B4" modified="2023-09-13" version="1">MSNYAVVMCSLCMGGVMFIVGAIQIVAWVLCFEFCLTESTTGGNFYLGLITGFLAIAVGVLGISYLCFLRSCLVIISLIVDVPLVFASSIALVISAVSITEELRNLGGGAFYLVVQSVMSGIIALFSTIFAVQYCCIQNRSLKEEDADDEERTAGVRRGPERYNPYVDDDSRILPNGNFSGVPRILTQNYIVSTPTSRPSGSSDSNVNRQERGRPGNRSNPTQQRATDRLGRTASEYLGRRSPKSRSPRYQPNGRSRGSRSPRDSGTIGSFQMGPIIGNGIAGGSREQLTGNTRNHEPYGLANDWLPDFDTSEVMDNEARYGDNSNYFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1GX56</accession>
    <name>A0A9Q1GX56_HOLLE</name>
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        <recommendedName>
            <fullName evidence="4">Bridge-like lipid transfer protein family member 1 C-terminal domain-containing protein</fullName>
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        <name evidence="5" type="ORF">HOLleu_30659</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
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        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
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        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="term" value="P:synaptic vesicle endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="BLTP1_C"/>
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        <property type="entry name" value="BLTP1_M"/>
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            <end position="3585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="3629"/>
            <end position="3638"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="3639"/>
            <end position="3648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="3934"/>
            <end position="3944"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="3993"/>
            <end position="4022"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="4234"/>
            <end position="4252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="4547"/>
            <end position="4563"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8028437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="4660" mass="516250" checksum="77932EEBB9C9C059" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H2H9</accession>
    <name>A0A9Q1H2H9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_27111</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H2H9"/>
    <dbReference type="OrthoDB" id="416710at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48312">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48312:SF1">
        <property type="entry name" value="SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8030643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="108" mass="12386" checksum="E153A63B2F7E81FC" modified="2023-09-13" version="1">MNCGNPYWSIDIPVPDRLYAFWQYVKNNIEPHPVVIDADDLQNHPQQILRKYCEAVGIPFKIRYLSWDESKEPWKRINGPLHLISEGADAFVTAISSSSFMPVSSQPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H2P0</accession>
    <name>A0A9Q1H2P0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Thiaminase-2/PQQC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_26669</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H2P0"/>
    <dbReference type="OrthoDB" id="6062485at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006772">
        <property type="term" value="P:thiamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.910.10">
        <property type="entry name" value="Heme oxygenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016084">
        <property type="entry name" value="Haem_Oase-like_multi-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004305">
        <property type="entry name" value="Thiaminase-2/PQQC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03070">
        <property type="entry name" value="TENA_THI-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48613">
        <property type="entry name" value="Heme oxygenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thiaminase-2/PQQC" evidence="1">
        <location>
            <begin position="14"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8030300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="119" mass="13781" checksum="0F76A94F73A0DC02" modified="2023-09-13" version="1">MFKTWSIGEPKGIYLNPACQAYTDFIARVAVDRDPVYFIVANIPCIRLWPWIGEKFKQSGKSSGLYQKWVDENFDPNSTGYREWEAYVNNSRHIDVTTARAIYHECMKGELGFFGSIKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H3C0</accession>
    <name>A0A9Q1H3C0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Fibrinogen C domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_24134</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H3C0"/>
    <dbReference type="OrthoDB" id="7250310at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00087">
        <property type="entry name" value="FReD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19941">
        <property type="entry name" value="TIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050373">
        <property type="entry name" value="Fibrinogen_C-term_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040941">
        <property type="entry name" value="GGGWT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143">
        <property type="entry name" value="FIBRINOGEN/TENASCIN/ANGIOPOEITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00186">
        <property type="entry name" value="FBG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040203905" evidence="1">
        <location>
            <begin position="24"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="2">
        <location>
            <begin position="40"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="2">
        <location>
            <begin position="288"/>
            <end position="509"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8033777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="509" mass="58377" checksum="142773C7ED4E0FC8" modified="2023-09-13" version="1">MLFYLYRVNQFVVFCWMTSILGAQQMSTQQANQGTSYFFYQRPEFPRDCEEVHNQCSSDTTSGVYLIKPDGYPEPFEVYCNNSNSQGWTVILRRNDGHVDFCRNYDEYKERFGFLSHEFWIGNDKLSYFTNQKNYELRIDFTNSEGSSCYATYNRFRTSDEFSNFKFTNIGQYSGNADDCITLCPRNAHPGDCTCQKTCANPSSCLDACSSDDTCICSDGFYLQGGECIPLIECPTGCSVDGIDIPEGGSYVNADCTRRGVCTNSHLIWDDTYSCSVNEVCEERNNVRQCYSDASDCLDIYNAGFSESGVYTVMPTNWPGSPFQVYCNMTEGGGWTVLQRRLDGTVDFYRNWTSYKEGFGSVDHEHWLGNDKLHYLTNQKRYTIRIDLVNLDNLPHFANFDFFRINDENDNYRLSELGTYGGTAGDSLSYHHNQQFSTYDQDHDSVSYDSLSYNCAVIHAGAWWYKSCIGCNLNGLYRSVLPCSDEYVFWYNVPVNNCNKFAEMKIRPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H9D2</accession>
    <name>A0A9Q1H9D2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_17850</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H9D2"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8037111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="81" mass="9097" checksum="44BF7EE0EC5B6181" modified="2023-09-13" version="1">MAFRACRISHNVKAPCRTPLGEESLRVWGDGPVCEASVERKAYTESVTLSVAAKLVDTEYAIGEWVFMSYSLIFSVNLYFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H9S6</accession>
    <name>A0A9Q1H9S6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TIR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_18599</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H9S6"/>
    <dbReference type="OrthoDB" id="10037120at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33480">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33480:SF1">
        <property type="entry name" value="TYR RECOMBINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13676">
        <property type="entry name" value="TIR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TIR" evidence="2">
        <location>
            <begin position="124"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="TIR" evidence="2">
        <location>
            <begin position="273"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="672"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="693"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="706"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="723"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="735"/>
            <end position="751"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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            <begin position="764"/>
            <end position="780"/>
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        <location>
            <begin position="781"/>
            <end position="790"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="793"/>
            <end position="809"/>
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    </feature>
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        <location>
            <begin position="831"/>
            <end position="847"/>
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    </feature>
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        <location>
            <begin position="881"/>
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        </location>
    </feature>
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        <location>
            <begin position="915"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="929"/>
            <end position="943"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8037708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="949" mass="107259" checksum="4DBF8042DEAA8F4F" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HC00</accession>
    <name>A0A9Q1HC00_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_14767</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HC00"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8040470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="84" mass="9396" checksum="78E69BCFCE785C8F" modified="2023-09-13" version="1">MEEFVMMRQGNVSVLQDSWEQTVLQLVEEIGLDTAVSFSVAMMEMMKIPAVVYSFVSWIRLDVDVTLDGKISAVTTVGSNVRVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HDR7</accession>
    <name>A0A9Q1HDR7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transposase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_12400</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome 5"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN CBG10204"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SI:CH211-108C17.2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="29"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="149"/>
            <end position="190"/>
        </location>
    </feature>
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        <location>
            <begin position="40"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAJ8041548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="399" mass="45051" checksum="8752EB6E89CA3507" modified="2023-09-13" version="1">MREKMGVAVGQRRLLPDAFPRVKKRKAVDDVAAVSGEADDASKKQRFSSESRRRQKEIEELIATSDQVKTAELAENIAKAETVNAQWKQLVCEKSSQTKKMEIPQQKVYRRSAAIQVKPNMKNKETQTVIVPEGKTFREMETQCSLIQPQSPEKETFQDGDDELSDIGGRSPCESEEYVPRGDARADSPGYSAKYGSNSLMELRRSKILDVVLVQKNEVSSSNAMELEGLKRVIRWMLDIGVKSGLAIDCFVTDRHLQVAKWVRENLVPLGIQHYYDVWHIAKSIKKQLKALANRRGCEAILKWQHSIINHVYWAASSTPEADQELILEKFLSIGNHVQNVHTGHQGERFTECAHPPISDEEERTKSWLEPVCLHGSHHLDFVAVSCPPFGLARTGKVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HE08</accession>
    <name>A0A9Q1HE08_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Hyalin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_13579</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8042510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="10"/>
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        <property type="entry name" value="Protein delta homolog 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Laminin"/>
        <property type="match status" value="11"/>
    </dbReference>
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        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CUB_dom"/>
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        <property type="entry name" value="EGF"/>
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        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
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        <property type="entry name" value="HYR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
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        <property type="entry name" value="FI04643P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24273:SF32">
        <property type="entry name" value="HYALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HYR"/>
        <property type="match status" value="42"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="11"/>
    </dbReference>
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        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="6"/>
    </dbReference>
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        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="8"/>
    </dbReference>
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        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="11"/>
    </dbReference>
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        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="6"/>
    </dbReference>
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        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50825">
        <property type="entry name" value="HYR"/>
        <property type="match status" value="42"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="1 6" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="4285"/>
            <end position="4306"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="9">
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            <begin position="12"/>
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        </location>
    </feature>
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        </location>
    </feature>
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        </location>
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        </location>
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        </location>
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            <begin position="730"/>
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        </location>
    </feature>
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        </location>
    </feature>
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        <location>
            <begin position="898"/>
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        </location>
    </feature>
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        <location>
            <begin position="981"/>
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        </location>
    </feature>
    <feature type="domain" description="HYR" evidence="11">
        <location>
            <begin position="1065"/>
            <end position="1148"/>
        </location>
    </feature>
    <feature type="domain" description="HYR" evidence="11">
        <location>
            <begin position="1149"/>
            <end position="1232"/>
        </location>
    </feature>
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        <location>
            <begin position="1233"/>
            <end position="1316"/>
        </location>
    </feature>
    <feature type="domain" description="HYR" evidence="11">
        <location>
            <begin position="1317"/>
            <end position="1400"/>
        </location>
    </feature>
    <feature type="domain" description="HYR" evidence="11">
        <location>
            <begin position="1401"/>
            <end position="1484"/>
        </location>
    </feature>
    <feature type="domain" description="HYR" evidence="11">
        <location>
            <begin position="1485"/>
            <end position="1568"/>
        </location>
    </feature>
    <feature type="domain" description="HYR" evidence="11">
        <location>
            <begin position="1569"/>
            <end position="1652"/>
        </location>
    </feature>
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        <location>
            <begin position="1653"/>
            <end position="1736"/>
        </location>
    </feature>
    <feature type="domain" description="HYR" evidence="11">
        <location>
            <begin position="1737"/>
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        </location>
    </feature>
    <feature type="domain" description="HYR" evidence="11">
        <location>
            <begin position="1821"/>
            <end position="1904"/>
        </location>
    </feature>
    <feature type="domain" description="HYR" evidence="11">
        <location>
            <begin position="1905"/>
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        </location>
    </feature>
    <feature type="domain" description="HYR" evidence="11">
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            <begin position="1989"/>
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        </location>
    </feature>
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            <begin position="2073"/>
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        </location>
    </feature>
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            <begin position="2157"/>
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        </location>
    </feature>
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        <location>
            <begin position="2241"/>
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        </location>
    </feature>
    <feature type="domain" description="HYR" evidence="11">
        <location>
            <begin position="2325"/>
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            <begin position="2911"/>
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    <feature type="domain" description="EGF-like" evidence="10">
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        <source>
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        <source>
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        </source>
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        </source>
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        </source>
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            <dbReference type="EMBL" id="KAJ8042510.1"/>
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            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
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    <sequence length="4327" mass="448072" checksum="97E3E8A651EB2C63" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HEI4</accession>
    <name>A0A9Q1HEI4_HOLLE</name>
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        <recommendedName>
            <fullName evidence="4">SCAN box domain-containing protein</fullName>
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        <name evidence="2" type="ORF">HOLleu_10418</name>
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        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Holothuroidea</taxon>
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        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
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        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
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            <begin position="1"/>
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    </feature>
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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            <dbReference type="EMBL" id="KAJ8043364.1"/>
        </source>
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        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
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            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="209" mass="25498" checksum="BEDC382CA4908E2E" modified="2023-09-13" version="1">MNKEIRLKEMEREREREEREMQMQMQKEKEEREMQMQMQREKEAREHEFRLKQLELGVIKGSDPKIGLDTGGLFDVSKHVKFVPKFQEDNVEKFFNHFEKLGEQLKWPRDKWSILIQSNFTGKAQEVYSALSIEDSMDYDKVKKAILQAYELVPEAYRQKFRKYRKADTQTYVEFAYQKERHFDRWCASKKVSTFDTLRQLMLVEEFKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
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    <name>A0A9Q1HEU3_HOLLE</name>
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            <fullName evidence="2">Uncharacterized protein</fullName>
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        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
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        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
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    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
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            <end position="49"/>
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            <end position="41"/>
        </location>
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        </source>
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        <source>
            <dbReference type="EMBL" id="KAJ8043350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="146" mass="15762" checksum="637388CDAA173221" modified="2023-09-13" version="1">MSEGTQAQPLPEGVTPELSAFILQAVAEAQSRPTTFTPSPQTHKETDDDASSLFGTLLGNTEATPTAVNSMLDDVAQEFLPEDSGPSLQQKIADIISKMLSGTLSETKLQEKCNLHLKPQNCERLQPTRVNQLVGINCAVPPETWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HFU3</accession>
    <name>A0A9Q1HFU3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Calcineurin B-likeous protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HOLleu_07084</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Nanhai2018</strain>
            <tissue evidence="18">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the calcineurin regulatory subunit family. CHP subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HFU3"/>
    <dbReference type="OrthoDB" id="191686at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051875">
        <property type="entry name" value="Calcineurin_B_homologous"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46002">
        <property type="entry name" value="EG:114D9.1 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0519">Myristate</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="EF-hand" evidence="17">
        <location>
            <begin position="107"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="17">
        <location>
            <begin position="148"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00038164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ8044355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="192" mass="21894" checksum="6AC96C00B66F80EE" modified="2023-09-13" version="1">MGSKTSLLLQEEEIIQISRETGFTPNQISRLYSRFISLDKSDTGRLSPSDLMRIPELAINPLGSRIVEAFFVNCGDDSETINFRQFVKTLAVFRPQRRKEEPKEINSRDAKLRFAFTIYDLEKTGTVSSDEVLVILRMMVGSNITEDQLQAIAKRTMNEADQNGTGRITFDDFSKALERVDAEKIMSIRFLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HFY1</accession>
    <name>A0A9Q1HFY1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_10701</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HFY1"/>
    <dbReference type="OrthoDB" id="16287at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8043558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="97" mass="10778" checksum="F97C7EE49E651D29" modified="2023-09-13" version="1">MSIVLHKNTPHLCLFALKDIYPESELRFDYGVKNLPWRKKSSSKRKQGAVQSFCAKKAKGSTSDGNFADSAANVHPSLQSMSFCGLNNKVGVVFDLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HHS3</accession>
    <name>A0A9Q1HHS3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">NTR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_06019</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HHS3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008191">
        <property type="term" value="F:metalloendopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001820">
        <property type="entry name" value="TIMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008993">
        <property type="entry name" value="TIMP-like_OB-fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00965">
        <property type="entry name" value="TIMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50242">
        <property type="entry name" value="TIMP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040364193" description="NTR domain-containing protein" evidence="5">
        <location>
            <begin position="32"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared with metalloproteinase partner</note>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="32"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="34"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="46"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR601820-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR601820-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8047114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="195" mass="22023" checksum="F86020FA5DDACDE5" modified="2023-09-13" version="1">MLMICGTMDILRNFCLSMIIVVFCLPSFVQSCTCPQSVESFEMKFCLADYSYLGEVKQVHLNEDEGTLRMSFEVIVKSIYHGMEDIDEGTKLAITTSMSETGCGLPGLSRGLHLFQGRGSFKDISKCDYVELYENVHPSTKENLSIGSYSKLCAYFYNNICLSATMRSEVTVDETTPFLEETTPYFDDYTTDDFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HIS9</accession>
    <name>A0A9Q1HIS9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Sm protein F</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_05187</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Plays a role in pre-mRNA splicing as a core component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the snRNP Sm proteins family. SmF/LSm6 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HIS9"/>
    <dbReference type="OrthoDB" id="409625at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034715">
        <property type="term" value="C:pICln-Sm protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01722">
        <property type="entry name" value="Sm_F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000011">
        <property type="entry name" value="small nuclear ribonucleoprotein F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016487">
        <property type="entry name" value="Lsm6/sSmF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034100">
        <property type="entry name" value="Sm_F"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11021:SF0">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11021">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN F SNRNP-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006609">
        <property type="entry name" value="snRNP_SmF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 11" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11 13" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 11" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Sm" evidence="12">
        <location>
            <begin position="8"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00058057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR006609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8046501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="88" mass="9941" checksum="00E683C282C8CD6F" modified="2023-09-13" version="1">MSQSMPLNPKPFLNGLTGKPVMVKLKWGMDYKGYLVSVDGYMNLQLANTEEYIDGAFTGNLGEVLIRCNNVMYIRGVEEEEEDGEMKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HJS5</accession>
    <name>A0A9Q1HJS5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase A2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_02487</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HJS5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF13">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="62"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8049649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="351" mass="38128" checksum="918DFE8F81570CF5" modified="2023-09-13" version="1">MLVEEFKRCVNDDIKTHLEENKADNLKEVANLADQYALTHKFGKVGQTQNKGQFSKPNKKYGNSSGTTGNSGSQSGSISPTNSGKKQPDSSRQKNDQVSSNKAAFKDITCGFCHKKGHTMANCFTLAKLKETETASNALANVENSSEVVVTLRPNSNDKIKEEYLPFVSEGFISLDGNSAHPPVKIKILRDTGATQSLLLDGVLPLSDSTSTGANVLSQGVECGFISVPLHKINLKSDLVSGSVIVGVRPTLPVKGVSLLLGNDLAGGKVVANAILTDKPCDYNNTEQLEKEFPNLFLPVLSLELCHKSSPWMIILNIHPPKVRIQFPKVEQVGNHVRMIILNIHSLKMRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HK57</accession>
    <name>A0A9Q1HK57_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_01261</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HK57"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8048795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="167" mass="19084" checksum="560022A0A5CD2166" modified="2023-09-13" version="1">MKLSRWKTQRSLKKAHVPPSITLYQFLAHHEDCQLCVGEVCDLMAIQKYFQGKGYYVQRHGELLIAVILDEKGRHVIKRLEVGKNVQCEINGKEITIPDGCQTPKEATGFVQVAKLCTGNVDFEKLAAERKDAGFKNVNWDVVARVEPFYFTDHTKVVGNTIRQYKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HM56</accession>
    <name>A0A9Q1HM56_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_04126</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HM56"/>
    <dbReference type="OrthoDB" id="418615at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8050791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="146" mass="16868" checksum="5BC80A5FAA2032C1" modified="2023-09-13" version="1">MEIHMLLTSYHQFTDVAKQARKFHWNDFVKRVLELPLTEYTIKTEGIYITAGNLYLVQEHIHCNTLETYQASSRQYTHSKEETIPLYESEAMRLIINILKGMEFIQSYGVAICNFSVVYCSLCNGTVKIFAGDGTLHKDLLHAFLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HM99</accession>
    <name>A0A9Q1HM99_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Death domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_04249</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HM99"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01670">
        <property type="entry name" value="Death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000488">
        <property type="entry name" value="Death_dom"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50017">
        <property type="entry name" value="DEATH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Death" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8050878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="134" mass="15243" checksum="6F0CCB9782C17F51" modified="2023-09-13" version="1">MLLSWKRSNGLHATKKALDEALKKIDREDLIDKIDSFNDKTFLFLLSHCKFPFCEEVEKVSVVDIDNIDKGSCISSTEFFRKSNDSIEDEFLVSVSDSSMNIKYNASGEKLSTKQTYLFIFISNAGEMVVIILL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YCK8</accession>
    <name>A0A9Q0YCK8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SWIM-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_42548</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000088">
        <property type="protein sequence ID" value="KAJ8019089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YCK8"/>
    <dbReference type="OrthoDB" id="5988294at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="3">
        <location>
            <begin position="405"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="447"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="460"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8019089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="610" mass="70087" checksum="4449EF4EE92E53FA" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YCQ6</accession>
    <name>A0A9Q0YCQ6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_44961</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010001519">
        <property type="protein sequence ID" value="KAJ8017547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YCQ6"/>
    <dbReference type="OrthoDB" id="10067251at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="121"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8017547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="230" mass="26548" checksum="C9D7CAAB5C73C31C" modified="2023-09-13" version="1">MQRKNDKLDRLRAEDRVLKHLNHTLAPTSNIILRRNRSRHTSTRQTNIATLSNYTLSNGEQRLLSRGLSFCPSRDINPVDLCQDMEQYLRRIKLKEYFQNDDTNSSPQTPLSREPSHKKSNWTPPSGGNQYIDSYVQTARGHLDRFILDNHHHSNKDNLTVNERKAIKTLRSNFDIAIKPADKGAPSLLWTLLIMWPKLTPNLATQPSTKNSRMILLNFLNLNLLNSLTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-04-02" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YF64</accession>
    <name>A0A9Q0YF64_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Protein NLRC5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_42120</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000033">
        <property type="protein sequence ID" value="KAJ8019334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="427518at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01120">
        <property type="entry name" value="RecA-like_superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.220.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000906">
        <property type="entry name" value="ZU5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46844">
        <property type="entry name" value="SLR5058 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46844:SF1">
        <property type="entry name" value="SLR5058 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00791">
        <property type="entry name" value="ZU5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51145">
        <property type="entry name" value="ZU5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040274611" evidence="2">
        <location>
            <begin position="19"/>
            <end position="1417"/>
        </location>
    </feature>
    <feature type="domain" description="NACHT" evidence="3">
        <location>
            <begin position="226"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="ZU5" evidence="4">
        <location>
            <begin position="1132"/>
            <end position="1271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="530"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="920"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="538"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="943"/>
            <end position="960"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8019334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1417" mass="160442" checksum="6DA644324B2A8105" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YHZ5</accession>
    <name>A0A9Q0YHZ5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Hexosyltransferase</fullName>
            <ecNumber evidence="9">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_37904</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Golgi apparatus</location>
            <location evidence="1 9">Golgi stack membrane</location>
            <topology evidence="1 9">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the chondroitin N-acetylgalactosaminyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="9"/>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YHZ5"/>
    <dbReference type="OrthoDB" id="431432at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047238">
        <property type="term" value="F:glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008428">
        <property type="entry name" value="Chond_GalNAc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051227">
        <property type="entry name" value="CS_glycosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12369">
        <property type="entry name" value="CHONDROITIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12369:SF45">
        <property type="entry name" value="HEXOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05679">
        <property type="entry name" value="CHGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8022883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="551" mass="63653" checksum="093E6F71C1A75431" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YIZ7</accession>
    <name>A0A9Q0YIZ7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Helix-turn-helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_35852</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YIZ7"/>
    <dbReference type="OrthoDB" id="10025388at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058912">
        <property type="entry name" value="HTH_animal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21301:SF12">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21301">
        <property type="entry name" value="REVERSE TRANSCRIPTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26215">
        <property type="entry name" value="HTH_animal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helix-turn-helix" evidence="1">
        <location>
            <begin position="106"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF26215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8023410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="272" mass="32071" checksum="61BA1D480E6F343D" modified="2023-09-13" version="1">MGSQASLEICDIFMHKLEKSILPTDPRIIRWFRYRDDILLHFNGNISELSVLVNKMNKIHDTIKFTMEASHESVTYLDLTIFKGKNFETQGRLDTKVYTKPTETYQYLDRTSAHPLATFKGFIKGEVLRYARLSNNENDFVSKRDNFKEKLLARNYTAQELDRAIKGVSYRERSDLLLPKLREENIPLVFKTTCTPHIKTKELKEIFLRHWHLISDDPNLCQIFPKPPFLAYKRAHNLKDYLVKSRFKSNGDLETFHEVYDDKYLDTLIEAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YKF7</accession>
    <name>A0A9Q0YKF7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetraspanin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_36694</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the tetraspanin (TM4SF) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8024067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YKF7"/>
    <dbReference type="OrthoDB" id="438211at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1450.10">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000301">
        <property type="entry name" value="Tetraspanin_animals"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF544">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002419">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00259">
        <property type="entry name" value="TMFOUR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="165"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8024067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="198" mass="22557" checksum="88EA42430724BE2E" modified="2023-09-13" version="1">MATTIVGLFAKVILFVVNFMFWYFGVVLLLLLAEVVCTVLLFAYNEEVKTFANETLSDFILHDYDQGDNQGITIAINQIQNKFKCCGAQSDDDWLRSEWYNRTKLQYETSTAGPRGYVQIVPTSCCLYPNGTAEEIKLCNDPVLDDSRFTDGCYNVLFRVFEDHIWIIGGILIGVAFFQIVLLIMAVVLLRNLLDDYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YM35</accession>
    <name>A0A9Q0YM35_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_38242</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8023144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YM35"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8023144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="112" mass="12596" checksum="1C152DB59BC79820" modified="2023-09-13" version="1">MLQQVTLGHTTSGMNTCSLLIKAVSLPSVGSTYDPRVSPFNRTKTFHRNKSQPMRDDVNYLSQKVMTSGFSTRRNTGDENRPVKHGGNDKYLVNSKIHAPQSFIMTSREPHM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YN59</accession>
    <name>A0A9Q0YN59_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_39292</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YN59"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="CDD" id="cd00185">
        <property type="entry name" value="TNFRSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.10">
        <property type="entry name" value="Tumor Necrosis Factor Receptor, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003410">
        <property type="entry name" value="HYR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001368">
        <property type="entry name" value="TNFR/NGFR_Cys_rich_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24273">
        <property type="entry name" value="FI04643P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24273:SF32">
        <property type="entry name" value="HYALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02494">
        <property type="entry name" value="HYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00020">
        <property type="entry name" value="TNFR_c6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00208">
        <property type="entry name" value="TNFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50825">
        <property type="entry name" value="HYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00652">
        <property type="entry name" value="TNFR_NGFR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50050">
        <property type="entry name" value="TNFR_NGFR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040424669" evidence="5">
        <location>
            <begin position="24"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="247"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="TNFR-Cys" evidence="6">
        <location>
            <begin position="69"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="repeat" description="TNFR-Cys" evidence="2">
        <location>
            <begin position="69"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="HYR" evidence="7">
        <location>
            <begin position="148"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="289"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="419"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="326"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="350"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="419"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="445"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="70"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="92"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8021946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="584" mass="64912" checksum="CE97F59C4826312C" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YN90</accession>
    <name>A0A9Q0YN90_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Dephospho-CoA kinase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_34323</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YN90"/>
    <dbReference type="OrthoDB" id="247245at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004140">
        <property type="term" value="F:dephospho-CoA kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02022">
        <property type="entry name" value="DPCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001977">
        <property type="entry name" value="Depp_CoAkinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10695:SF46">
        <property type="entry name" value="BIFUNCTIONAL COENZYME A SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10695">
        <property type="entry name" value="DEPHOSPHO-COA KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01121">
        <property type="entry name" value="CoaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51219">
        <property type="entry name" value="DPCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="82"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8024420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="102" mass="11464" checksum="CC97C6174335D887" modified="2023-09-13" version="1">MNQIVVVYCDQQTQLNRLISRNNLNEEEAQNRIHSQVPLVEKCHMADHVIDNSGSLESTKEAVTKLHQTFVSSNAHWKLRSVVLAIAFIVVGLSALTLRSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YNX4</accession>
    <name>A0A9Q0YNX4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">TWiK family of potassium channels protein 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_33573</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the two pore domain potassium channel (TC 1.A.1.8) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YNX4"/>
    <dbReference type="OrthoDB" id="297496at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015271">
        <property type="term" value="F:outward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022841">
        <property type="term" value="F:potassium ion leak channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030322">
        <property type="term" value="P:stabilization of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003280">
        <property type="entry name" value="2pore_dom_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11003:SF330">
        <property type="entry name" value="POTASSIUM CHANNEL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11003">
        <property type="entry name" value="POTASSIUM CHANNEL, SUBFAMILY K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01333">
        <property type="entry name" value="2POREKCHANEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5 8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="24"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="123"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="156"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="269"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="324"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="11">
        <location>
            <begin position="121"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="11">
        <location>
            <begin position="275"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="204"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="220"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF07885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8025882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="364" mass="41547" checksum="4ED8B519394DC476" modified="2023-09-13" version="1">MAQAKNTPQNGILRKLVKLLRVTFPHVVLLSSYLAYLILGSFSFCYLEGHFRQSDLETYHHQMNNTRTDIMEMLSNSKCDDNEDKHRDKLQEVNRLLDKLEEDSRRPEWKAGRMMSVRANRTWTFSSSMLFCMTTITTIGYGDIVPTSKPGRAFCVIYALIGIPFTLLLLADVGDLLARAVIWCCRKLGVHNCSRRLKTQCSRSTPSQTFKQNRRPNVRSRTENRTENEKAVTSHHNMGVMGESSSAEDHKGSGHVCTRDGSCQQGVPIFVILFILVSYICGGSWLLMMIEHWTYGEAMYFMIITFTTIGYGDLVPANLYEHDAFVFCIFFTLLGMAMMSLTIALLREKVVKSFTFIYSKLNLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YQA6</accession>
    <name>A0A9Q0YQA6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_33149</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8025560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YQA6"/>
    <dbReference type="OrthoDB" id="5350595at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019384">
        <property type="entry name" value="FHIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21705:SF12">
        <property type="entry name" value="FHF COMPLEX SUBUNIT HOOK-INTERACTING PROTEIN C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21705">
        <property type="entry name" value="RAI16 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10257">
        <property type="entry name" value="RAI16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8025560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="139" mass="16119" checksum="D5025DED1A8BE584" modified="2023-09-13" version="1">MFNRFAAALHQAVEALAPSQSLLEDFIFHWKAITHYFINNKDDKCPVQESNISSHLDNMLVILEQEEYEMEKNQTGPCMEHLLQHKILETLQTLGKADCPPGMKQLVLTFFTNLLGKIQQPLLPHVNVYKPVHVSRWFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YTK7</accession>
    <name>A0A9Q0YTK7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_31645</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YTK7"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8026727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="59" mass="6844" checksum="4BB53D56F7310F51" modified="2023-09-13" version="1">MEKGFCPNFRIVHSGRSRKGGCKTTITEQIVHSLSVTQRQHLFRINQFISLHGWISDFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1B8E9</accession>
    <name>A0A9Q1B8E9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein stu1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_45267</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010002256">
        <property type="protein sequence ID" value="KAJ8017376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1B8E9"/>
    <dbReference type="OrthoDB" id="9950881at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="103"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8017376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="220" mass="26389" checksum="5C923E69B1C45DEC" modified="2023-09-13" version="1">MNARMNPRMNPRMNVRMNPRMNVRMNVRMNPRMNVRMNPRMNPRMNVRMNPRMNVRMNPRMDVRQNPRVNVRMNVRQNPRMNVRMNPRMNPRMNLRMNTRIDECEDESEDECEDESEDESEDESEDESEDECEDESEDESEDECEDESEDEYEDESEDESEDECEDESEDECEDESEDECEDESGDESEDECKDECEAESEEKSEDESEDESEDECEDES</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1B975</accession>
    <name>A0A9Q1B975_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">E3 ubiquitin-protein ligase TRIM33</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_42898</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000159">
        <property type="protein sequence ID" value="KAJ8018876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1B975"/>
    <dbReference type="OrthoDB" id="10113904at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047153">
        <property type="entry name" value="TRIM45/56/19-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25462">
        <property type="entry name" value="BONUS, ISOFORM C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25462:SF296">
        <property type="entry name" value="MEIOTIC P26, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101898">
        <property type="entry name" value="NHL repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="B box-type" evidence="2">
        <location>
            <begin position="17"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8018876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="647" mass="73565" checksum="0BA288BCFB934112" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1B9K3</accession>
    <name>A0A9Q1B9K3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Fibrinogen-like protein A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_43821</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000480">
        <property type="protein sequence ID" value="KAJ8018275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1B9K3"/>
    <dbReference type="OrthoDB" id="5945673at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037579">
        <property type="entry name" value="FIB_ANG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040941">
        <property type="entry name" value="GGGWT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47221">
        <property type="entry name" value="FIBRINOGEN ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47221:SF6">
        <property type="entry name" value="FIBRINOGEN ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00186">
        <property type="entry name" value="FBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040133521" evidence="7">
        <location>
            <begin position="27"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="8">
        <location>
            <begin position="48"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8018275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="213" mass="23992" checksum="C9528BF22EAC61D8" modified="2023-09-13" version="1">MASSNKLYMVSFALGVYSFFLDGLSAEGNDQLPSESAIDGQGSTFFFYQKPQYPRDCTEVRDSCSSSVSSGIYLIKPDGYEEPFEAFCDNEIDSGGWTVILRRLDGSVSFKRNWIDYKRGFGFLSTELWLGNEKVSYLTNQAKYELRIDITLDNGTSCNVKYNAFRISDEGSEYTMSTVGNIGDILNSTGFSVARTAERYHFNKTKHDLRQAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BB49</accession>
    <name>A0A9Q1BB49_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Histone-lysine N-methyltransferase EZH2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_43334</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000273">
        <property type="protein sequence ID" value="KAJ8018589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BB49"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8018589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="143" mass="16476" checksum="8A25BE5378A09BE6" modified="2023-09-13" version="1">MYSTSTTKGTLTLPEAQKAVYRWHRLVSEAIRFDSRLDQALDRERSSGLMDPSELRLYDSLESWIVSHILYLLVQFFTSVRIVVLPEITYSLWLILDFVVDATRKGNKIHFANHSVNPNCRAKGEMKREMERGEGNWGLGIEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BBB7</accession>
    <name>A0A9Q1BBB7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">BRCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_42473</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000077">
        <property type="protein sequence ID" value="KAJ8019143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BBB7"/>
    <dbReference type="OrthoDB" id="5997366at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16589">
        <property type="entry name" value="BRCT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRCT" evidence="1">
        <location>
            <begin position="586"/>
            <end position="690"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8019143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="811" mass="91856" checksum="E396845750429A17" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BBL5</accession>
    <name>A0A9Q1BBL5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_39198</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BBL5"/>
    <dbReference type="OrthoDB" id="10570013at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040247692" description="Ig-like domain-containing protein" evidence="1">
        <location>
            <begin position="26"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8021876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="452" mass="49496" checksum="4131616B36FBC7A9" modified="2023-09-13" version="1">MEALLHIRTVFLLLFLRVSLNGAAGVTISFTGRNVYSVPFFDTDGFIKKSFLTYKGLSSEVTLRCETPPNSGPVSIFKGPWSNPSLLVDRGTPVIMDPNLSFSELVPSGYEIAIDTITSENAGTYYCFSSESVSDREFTERSSFVELFVEEDRTALFCLMHSYTDSIFFSDVDDAVLQCIANIDDLRESLISVYQRDDETQETSEIDSSLSFPVQSMFVEFFQAAVIIYEIPGDAGTDILSFNNSVLICERSPDSGLCGNVEPLKIFTDLKVLLDLANFDMEADEKSLKFVCRTFPNVVATNLTWTVSEQVYSTSRVSQTSDGSELVLENIDLDKISGKDFNVSCSVTIGGRETITQETRLQVDESNGMVIVGGEIVTQKNTVQPGGGNGEEVTVGESNGMVTVGGGETVTQKNTVQQGGGNGEEENIAPHLRTSSFISISIVLTNFLLLFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BF20</accession>
    <name>A0A9Q1BF20_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_37838</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BF20"/>
    <dbReference type="OrthoDB" id="6502630at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8022834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="152" mass="16971" checksum="D631131CC446563A" modified="2023-09-13" version="1">MCYGKRKNPIVFDRGQRSSGVTGGQMLKTLSTYLKMCYGKRKNPIVFDRGQRSSGVTGGQMLKTLLTRYLKMRFGKRKNPIVFGGSQRSSGVTGGQTLKTLLTRYLKMRYGKRKSLIVFGGGQRSSGVTGGRMLKTLLTRYLKLGSLDKFHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BG44</accession>
    <name>A0A9Q1BG44_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Fatty acid synthase</fullName>
            <ecNumber evidence="1">2.3.1.85</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HOLleu_35446</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Nanhai2018</strain>
            <tissue evidence="15">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>acetyl-CoA + n malonyl-CoA + 2n NADPH + 2n H(+) = a long-chain fatty acid + (n+1) CoA + n CO2 + 2n NADP(+).</text>
            <dbReference type="EC" id="2.3.1.85"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.85" evidence="1"/>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8025280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BG44"/>
    <dbReference type="OrthoDB" id="10069075at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004312">
        <property type="term" value="F:fatty acid synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00833">
        <property type="entry name" value="PKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.366.10">
        <property type="entry name" value="Malonyl-Coenzyme A Acyl Carrier Protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001227">
        <property type="entry name" value="Ac_transferase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014043">
        <property type="entry name" value="Acyl_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014031">
        <property type="entry name" value="Ketoacyl_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014030">
        <property type="entry name" value="Ketoacyl_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016036">
        <property type="entry name" value="Malonyl_transacylase_ACP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032821">
        <property type="entry name" value="PKS_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020841">
        <property type="entry name" value="PKS_Beta-ketoAc_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050091">
        <property type="entry name" value="PKS_NRPS_Biosynth_Enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43775">
        <property type="entry name" value="FATTY ACID SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43775:SF7">
        <property type="entry name" value="FATTY ACID SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00698">
        <property type="entry name" value="Acyl_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16197">
        <property type="entry name" value="KAsynt_C_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00109">
        <property type="entry name" value="ketoacyl-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02801">
        <property type="entry name" value="Ketoacyl-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00827">
        <property type="entry name" value="PKS_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00825">
        <property type="entry name" value="PKS_KS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55048">
        <property type="entry name" value="Probable ACP-binding domain of malonyl-CoA ACP transacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52004">
        <property type="entry name" value="KS3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="9" id="KW-0520">NAD</keyword>
    <keyword evidence="7" id="KW-0521">NADP</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ketosynthase family 3 (KS3)" evidence="14">
        <location>
            <begin position="8"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00044883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS52004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ8025280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="846" mass="93491" checksum="53AD40EC3B52A6D9" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BGL0</accession>
    <name>A0A9Q1BGL0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF4709 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_34972</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BGL0"/>
    <dbReference type="OrthoDB" id="10027521at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040119">
        <property type="entry name" value="C10orf67-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031651">
        <property type="entry name" value="DUF4709"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22382">
        <property type="entry name" value="RIKEN CDNA 4921504E06 GENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22382:SF7">
        <property type="entry name" value="RIKEN CDNA 4921504E06 GENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15821">
        <property type="entry name" value="DUF4709"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4709" evidence="3">
        <location>
            <begin position="23"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="142"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="490"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="51"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="145"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="510"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF15821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8024918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="523" mass="60281" checksum="CC3074E389F7D61F" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BGM3</accession>
    <name>A0A9Q1BGM3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Tigger transposable element-derived protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_35032</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BGM3"/>
    <dbReference type="OrthoDB" id="10043687at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050863">
        <property type="entry name" value="CenT-Element_Derived"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004875">
        <property type="entry name" value="DDE_SF_endonuclease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006600">
        <property type="entry name" value="HTH_CenpB_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19303:SF74">
        <property type="entry name" value="POGO TRANSPOSABLE ELEMENT WITH KRAB DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19303">
        <property type="entry name" value="TRANSPOSON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03184">
        <property type="entry name" value="DDE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51253">
        <property type="entry name" value="HTH_CENPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH CENPB-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8024963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="265" mass="30280" checksum="9F80B13C6910756A" modified="2023-09-13" version="1">MKLSACGYPLSRREVITLATESAELLGTRAANGNQLSKGWFERFMRRWPQLRLAKAQKLSLKRAKATSKETVESYFKELEKIFVKYELTDKPHLIYNVDETGFQTEHEPTKVVGVRNQRLNSITSERGAITTVLACRNACGTALPPYFVFKGRRDDPRLMNGSIPGAKYTMSDSGWSNGAIFKLFLEEHFIPLLPQRQKDEHALLMYDGHASHISLPLIELAKEHKIILFVLPPHTSHALQPLDVGTFRPVKCRYSYECQKEMRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BGU5</accession>
    <name>A0A9Q1BGU5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_33721</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000017">
        <property type="protein sequence ID" value="KAJ8026000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BGU5"/>
    <dbReference type="OrthoDB" id="410155at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33273">
        <property type="entry name" value="DOMAIN-CONTAINING PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33273:SF4">
        <property type="entry name" value="ENDONUCLEASE_EXONUCLEASE_PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14529">
        <property type="entry name" value="Exo_endo_phos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="2">
        <location>
            <begin position="7"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="343"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8026000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="366" mass="40712" checksum="CFBA7E968266885C" modified="2023-09-13" version="1">MSNNYTRNLFLGDFNAKHPALGHDTTNRNGNALAEIANTHGLILLNDDTPTYYSNQGTPGVLDLALCSQLLSPSLFNFSVLQELSSDHLPFLINSISPLSTSTPGPYKLANTKNYNHTDWKAYTDALSLPPDPPLQNTADIDALVETLTLTIRTALDNNTPDPKPPPTHTHCTLPGHILAHIRNKNRLRKDYQRTRNPATKRLINRLTNDIKRLIQNHSAQRWEHACNQINDNTPPQIRWKIFKRLTGNAKKTPYPTLVSGNTFARTDPEKANLFNLTMDNIQRDLADPNFNPHTKALIDRVVALNNNALSPLPQIPPEPPPNNLTTPFTPLEVYQALKLRKNTSPGTDGIPFKALKLGPPPSSTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BI71</accession>
    <name>A0A9Q1BI71_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transducin beta-like protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_31306</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BI71"/>
    <dbReference type="OrthoDB" id="5414888at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034511">
        <property type="term" value="F:U3 snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19854">
        <property type="entry name" value="TRANSDUCIN BETA-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19854:SF15">
        <property type="entry name" value="TRANSDUCIN BETA-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="43"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="89"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="145"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="187"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="229"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8026479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="306" mass="33853" checksum="D1E10DACB0F379EC" modified="2023-09-13" version="1">MVGNNDEILDVRFLGPDESHLAVVSNSDQIKVFELATLNCQILTGHTDIIVAIDVFKKGTMLVSSSKDNSVRLWLMDGEDKTIQCIAVGKGHTHSVLSVVSARLSRKFFVSGGEDLTFKVWKISSENRKVSEKGQLSTLSVSYTEKAHDKSINSLAISPNDKLLATGSQDRTAKLWHLEQEAFLGSFRGHKRGIWCVQFSPTDQALATSSADGTIKIWSIADFSCVKTFEGHDASVLKVTFLTRGMQIMSSGSDGLLKLWTIKNNECIKTFDEHDDKVWAITPTKSEDYVVNCGTRRLLRARLPPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BIV9</accession>
    <name>A0A9Q1BIV9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transcription cofactor vestigial-like protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_32552</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BIV9"/>
    <dbReference type="OrthoDB" id="10050213at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011520">
        <property type="entry name" value="Vg_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15950:SF15">
        <property type="entry name" value="PROTEIN VESTIGIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15950">
        <property type="entry name" value="TRANSCRIPTION COFACTOR VESTIGIAL-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07545">
        <property type="entry name" value="Vg_Tdu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="109"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="185"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="116"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="185"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="199"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8027416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="434" mass="47914" checksum="BA1EC4B91AB46B08" modified="2023-09-13" version="1">MSCLEMMYQGASFQSYRSPFSLYPSRSKKKFGHYKMQDALLDGCPVGAPAVDFSSHSFPLQPYHHSPVPHSLTGTPNASILTSSSYPLMSHEQHHLLKLESVGYTELQPRTSEGSDVSKHGRQCSEGEDGDDVVPEAEYVNPRCVIFTFYSGDVNAVVDEHFKRALSQPSSFSREGVCRNKNDIQSKATDGKQNDSNKKTSISPVQSTVTVKEQTVQKGSDESSPTLMCRRKFPPSFWNSNYWREMKAPGPLVPFDGTNVHYLSGNGGNCFYQNAFNTNTAAAAAAVTPFRHSDTLFTADTYTTYHHRAAGIQSQSDPWHYAISAAAHHQSAFAHHKSGLGIHELSYGLTSGSAFNPRYSSLLIQPTVRSAMLPAVPGQMELPKAAGITDHWNTPGYNGLHSHSQHYPTEMTSYGIDSTTSLETHESPKDLFWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BJA0</accession>
    <name>A0A9Q1BJA0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_32814</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BJA0"/>
    <dbReference type="OrthoDB" id="7920740at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="60"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="13"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="62"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="80"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8027617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="102" mass="11389" checksum="D86C0AEBB9A49A6C" modified="2023-09-13" version="1">MQQSMADLQSEVKAMRSELQLMRDERRQYGGGQGRQGKGSPRPTTSNGDPTFTEEGTPICFKCRKPGHMRRNCPEKRGNTTKQNEVTTGNDQASEMGVALRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BK07</accession>
    <name>A0A9Q1BK07_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Periodic tryptophan protein 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_30173</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat PWP2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BK07"/>
    <dbReference type="OrthoDB" id="3142434at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034388">
        <property type="term" value="C:Pwp2p-containing subcomplex of 90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000216">
        <property type="entry name" value="Periodic tryptophan protein 2 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027145">
        <property type="entry name" value="PWP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007148">
        <property type="entry name" value="SSU_processome_Utp12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19858:SF0">
        <property type="entry name" value="PERIODIC TRYPTOPHAN PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19858">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04003">
        <property type="entry name" value="Utp12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="204"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="246"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="332"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="383"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="domain" description="Small-subunit processome Utp12" evidence="6">
        <location>
            <begin position="605"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="73"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="719"/>
            <end position="762"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8028046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="762" mass="85737" checksum="E2606EE9C22C1147" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BKM0</accession>
    <name>A0A9Q1BKM0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Rho GTPase-activating protein 26</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_30547</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BKM0"/>
    <dbReference type="OrthoDB" id="3183924at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01249">
        <property type="entry name" value="BAR-PH_GRAF_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004148">
        <property type="entry name" value="BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047234">
        <property type="entry name" value="GRAF_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047225">
        <property type="entry name" value="PH_GRAF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12552">
        <property type="entry name" value="OLIGOPHRENIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12552:SF1">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN GRAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16746">
        <property type="entry name" value="BAR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="2">
        <location>
            <begin position="266"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="227"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8028343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="366" mass="42955" checksum="4D47A8421AA74E52" modified="2023-09-13" version="1">MVLEGLEFEECFLDSPEFRQNLRIHEAEIEKTDKGIRDLIRTGKTLVEAAQNLSRAQRAFSQMLTNFKFECVEQKGNEEEVQISTSLQELGKLVGLIEDERDRMLEAANYDKLVSKLEEFRRDHIAIAREHKKSFDKQTERYCKSVDSHVKLTPKKNSDAELHSADLSLDSERRQYHRESLSYVYKLQEVQERKKFDFVENLVGFANNWLLFYTKGHEVSTNFKPYMTNLQLKLQSAKSEYEQKKKEADGVMHKMLMAPYDSKKQEGLHEGYLYVQFKRSWTKCYCTFNKNTKVLKMSMYSQTLPAVTGEQFFTVTSCTRKVEEINDRRFCFDITVTEKHSIVVIQAINKEERTQWMVAMGCKDPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BKP7</accession>
    <name>A0A9Q1BKP7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF4190 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_30529</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BKP7"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="168"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="217"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8028326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="240" mass="25935" checksum="7ECEF438229C5B03" modified="2023-09-13" version="1">MHQPMGDNNYAHNIPRAGNEGNISSHGRPEAHQQTETTPDFIYLEIQEPPASSTPTTDQRLQTPTAPVPSVVPQNQTTNSYQQTTYPPAAVNQGYVPVQQPPSYNELYGNGHPGFNPGPYTHQPQPFQTNYQSNVVVTTQPQPTPSNMVFVNSAPPRNTSQGLATASLVMAIIGIFCCFCGVFCTVPAIIFAVISLGNSTDPNKARTHANISMTCTVIGWISGIIIAILYFVLVISASTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BN76</accession>
    <name>A0A9Q1BN76_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Myeloid-derived growth factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_29231</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BN76"/>
    <dbReference type="OrthoDB" id="10061830at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001938">
        <property type="term" value="P:positive regulation of endothelial cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018887">
        <property type="entry name" value="MYDGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31230:SF1">
        <property type="entry name" value="MYELOID-DERIVED GROWTH FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31230">
        <property type="entry name" value="MYELOID-DERIVED GROWTH FACTOR MYDGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10572">
        <property type="entry name" value="UPF0556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040251392" evidence="1">
        <location>
            <begin position="22"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8029756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="158" mass="18008" checksum="4FA5C31F6D9C6DD3" modified="2023-09-13" version="1">MDTWKLAVVLLISYQLYLVNCDEDESMNHHYFNVQPGGEVHLLSRYLGPFECSFTYAAQGGTNEQWFFDLEADHDGKLYTCSIGRPSGSSYLVFSQFKVELYGAELKDAEIFSSQRDKLSEDEYTLDKEQNLVSSNEGVFKNQLARIHVLAQGVKTEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BNP1</accession>
    <name>A0A9Q1BNP1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Ammonium transporter 1 member 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_29367</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ammonia transporter channel (TC 1.A.11.2) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BNP1"/>
    <dbReference type="OrthoDB" id="534912at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008519">
        <property type="term" value="F:ammonium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097272">
        <property type="term" value="P:ammonium homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3430.10:FF:000008">
        <property type="entry name" value="Ammonium transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3430.10">
        <property type="entry name" value="Ammonium transporter AmtB like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029020">
        <property type="entry name" value="Ammonium/urea_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024041">
        <property type="entry name" value="NH4_transpt_AmtB-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11730">
        <property type="entry name" value="AMMONIUM TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11730:SF58">
        <property type="entry name" value="AMMONIUM TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00909">
        <property type="entry name" value="Ammonium_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111352">
        <property type="entry name" value="Ammonium transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0924">Ammonia transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="92"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="118"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="168"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="202"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="234"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="259"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="288"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="326"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="Ammonium transporter AmtB-like" evidence="9">
        <location>
            <begin position="1"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8029856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="477" mass="51623" checksum="2234B668B4C29D2B" modified="2023-09-13" version="1">MVSRKNEVNIMVKNSVDVIFGGLSYWIFGYAFSFGTDPGTNWFCGIGMFFTDSDDIENMGWIFAKFVFQASFATTATTIVSGAMAERTKLESYCLVSLFNTLIYCFPAHWVWAEEGIFFQMGAIDIAGAGAVHLVGGITGLIATIMLKPRRGRFDGSRSAEMSNPTNAILGMFMLWWGWLGFNCGSTFGISGGKWKLATRAAVTTLTASIGGGVTGIIGSRIVKPMGRKFDISFLNNGVLGGLVAITSLCALARPWEGLLIGTVGGLLAIFSPWAIEKMKIDDPVGVVGVHAVAAVWGLLSAGIFAEPDTIEAFNDRRGLIYGGGFELLGIQLLMSVTIIVWTALSSYLLLYIVDKSVGLRCSVEHEILGADIVEHGINNTTQEERKNISREFASLGLPEPFDKSGNYINADSKHKFPLAFPITTYCYGKRTSVFTVTGRRPSERTPSIDEPDGVTVARIHKNLQNEGATNLACIAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BNY4</accession>
    <name>A0A9Q1BNY4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">UDP-glucuronosyltransferase 2B33</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_29591</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8030024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BNY4"/>
    <dbReference type="OrthoDB" id="5835829at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008194">
        <property type="term" value="F:UDP-glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000021">
        <property type="entry name" value="UDP-glucuronosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000205">
        <property type="entry name" value="UDP-glucuronosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050271">
        <property type="entry name" value="UDP-glycosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043">
        <property type="entry name" value="EG:EG0003.4 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043:SF145">
        <property type="entry name" value="FI06409P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040401471" evidence="5">
        <location>
            <begin position="29"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="508"/>
            <end position="532"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8030024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="550" mass="62146" checksum="D30F2AC3DF77BF68" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BPV7</accession>
    <name>A0A9Q1BPV7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Soluble interferon alpha/beta receptor OPG204</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HOLleu_26983</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Nanhai2018</strain>
            <tissue evidence="18">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Counteracts the antiviral effects of host IFN-alpha/beta and key IFN-inducible proteins involved in viral RNA degradation suxh as host OAS1. Acts as a soluble IFN-alpha receptor and thus inhibits the interaction between host IFN-alpha and its receptor.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Interacts with host IFNA1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the interleukin-1 receptor family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BPV7"/>
    <dbReference type="OrthoDB" id="504170at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039502">
        <property type="term" value="P:symbiont-mediated suppression of host type I interferon-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015621">
        <property type="entry name" value="IL-1_rcpt_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11890">
        <property type="entry name" value="INTERLEUKIN-1 RECEPTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11890:SF44">
        <property type="entry name" value="X-LINKED INTERLEUKIN-1 RECEPTOR ACCESSORY PROTEIN-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01582">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01537">
        <property type="entry name" value="INTRLKN1R1F"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0244">Early protein</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0945">Host-virus interaction</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="3" id="KW-1090">Inhibition of host innate immune response by virus</keyword>
    <keyword evidence="5" id="KW-1114">Inhibition of host interferon signaling pathway by virus</keyword>
    <keyword evidence="9" id="KW-0922">Interferon antiviral system evasion</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="18" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0732">Signal</keyword>
    <keyword evidence="14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0899">Viral immunoevasion</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040485256" description="Soluble interferon alpha/beta receptor OPG204" evidence="15">
        <location>
            <begin position="29"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="348"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="17">
        <location>
            <begin position="72"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="17">
        <location>
            <begin position="147"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="TIR" evidence="16">
        <location>
            <begin position="402"/>
            <end position="541"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00041012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ8030533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="600" mass="67068" checksum="53D8E9CBD24AE75A" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BPW4</accession>
    <name>A0A9Q1BPW4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Reverse transcriptase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_27149</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10053045at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09274">
        <property type="entry name" value="RNase_HI_RT_Ty3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000003">
        <property type="entry name" value="Protein CBG25708"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.370:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 17.6-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000063">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 297-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000020">
        <property type="entry name" value="Transposon Tf2-6 polyprotein-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041373">
        <property type="entry name" value="RT_RNaseH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF15">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17917">
        <property type="entry name" value="RT_RNaseH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="8">
        <location>
            <begin position="471"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase catalytic" evidence="9">
        <location>
            <begin position="1012"/>
            <end position="1176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1272"/>
            <end position="1315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="8"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1292"/>
            <end position="1310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8030677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1327" mass="150240" checksum="4BFE199095DAF7A5" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BR69</accession>
    <name>A0A9Q1BR69_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_27865</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8031208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BR69"/>
    <dbReference type="OrthoDB" id="10037236at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8031208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="83" mass="9794" checksum="F196C6A28941BC7A" modified="2023-09-13" version="1">MLQQSRKVAQRVIGVSLPLLECLYHEKICNKVKQIMQDPTPLFEHYTYNHSGVRLCPPCTCRARYRYSLVPNSIRIFNSQVRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BSN2</accession>
    <name>A0A9Q1BSN2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Potassium voltage-gated channel subfamily KQT member 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="23">IKs producing slow voltage-gated potassium channel subunit alpha KvLQT1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">KQT-like 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">Voltage-gated potassium channel subunit Kv7.1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="29" type="ORF">HOLleu_25292</name>
    </gene>
    <organism evidence="29 30">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="29">Nanhai2018</strain>
            <tissue evidence="29">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Basolateral cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Membrane raft</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the potassium channel family. KQT (TC 1.A.1.15) subfamily. Kv7.1/KCNQ1 sub-subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="29">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BSN2"/>
    <dbReference type="OrthoDB" id="8879391at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051049">
        <property type="term" value="P:regulation of transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003008">
        <property type="term" value="P:system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000113">
        <property type="entry name" value="Potassium voltage-gated channel subfamily KQT member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000017">
        <property type="entry name" value="potassium voltage-gated channel subfamily KQT member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1910">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003937">
        <property type="entry name" value="K_chnl_volt-dep_KCNQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013821">
        <property type="entry name" value="K_chnl_volt-dep_KCNQ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005827">
        <property type="entry name" value="K_chnl_volt-dep_KCQN1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47735:SF14">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL SUBFAMILY KQT MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47735">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL SUBFAMILY KQT MEMBER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03520">
        <property type="entry name" value="KCNQ_channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01460">
        <property type="entry name" value="KCNQ1CHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01459">
        <property type="entry name" value="KCNQCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="20" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="19" id="KW-0407">Ion channel</keyword>
    <keyword evidence="17" id="KW-0406">Ion transport</keyword>
    <keyword evidence="18 26" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-0630">Potassium</keyword>
    <keyword evidence="10" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 26" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16 26" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <keyword evidence="14" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="130"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="164"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="205"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="270"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="337"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="27">
        <location>
            <begin position="131"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel voltage dependent KCNQ C-terminal" evidence="28">
        <location>
            <begin position="550"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="663"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="25">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="25">
        <location>
            <begin position="663"/>
            <end position="697"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00019342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00029687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00030121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00032659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="Pfam" id="PF03520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="EMBL" id="KAJ8031925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="707" mass="79385" checksum="3D8FDEB35F5A9191" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BUC9</accession>
    <name>A0A9Q1BUC9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">E3 ubiquitin-protein ligase TRIM56</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_23028</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8032935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BUC9"/>
    <dbReference type="OrthoDB" id="10250935at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19757">
        <property type="entry name" value="Bbox1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047153">
        <property type="entry name" value="TRIM45/56/19-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25462">
        <property type="entry name" value="BONUS, ISOFORM C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25462:SF296">
        <property type="entry name" value="MEIOTIC P26, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="16"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="6">
        <location>
            <begin position="95"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="6">
        <location>
            <begin position="156"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8032935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="678" mass="77614" checksum="F0F447B0AC6E5001" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BUJ7</accession>
    <name>A0A9Q1BUJ7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">D(2) dopamine receptor A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_23735</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BUJ7"/>
    <dbReference type="OrthoDB" id="6096660at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00637">
        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24243">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24243:SF208">
        <property type="entry name" value="PYROKININ-1 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="40"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="79"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="120"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="165"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="362"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="403"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="9">
        <location>
            <begin position="23"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8033483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="446" mass="49370" checksum="BEE45CE2B2202352" modified="2023-09-13" version="1">MAWTDNLAVSIFLAIISVVGVPGNVLVLKVYCRKARKLSTYVFISFMAVSDLFLCVLTPLHTAFILVTDPGKKQVLCSIEYFISASCRILSFLTSAVVAFDRYVAVCWPHTKRLSVPTSLILSMTCAVCTVVLSTPAFFVVSISLDEYDFISCKSRLDTHPFLSIVHIIVSSTVFTSAVGTVMCYTLVWRSIRNQRKVINLARQVPTKKAGNNFAVDVRYTTGGKKYTNMSSKVETLSIHFANTNQNINGTGPIEAGKLAGNDYELKVGKYKASNINVSEESTSKSTSLIPNTSLMLPQTTDVPKDKKILQHSLEKTPNLGMPCSSCDPTHGIEDVEKSPAITASTTRIKPSKLTVIVQRRITIMLFLIATISLTTMPIGILARLVPDSKLRDVKYTSEVLFVIIRVAEYVGFINNAVNPFIYSLFDKRFRKECFKTFTCVPPEEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BVQ7</accession>
    <name>A0A9Q1BVQ7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="21">Hephaestin-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">HOLleu_24081</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">Nanhai2018</strain>
            <tissue evidence="21">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the multicopper oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="61110at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:monoatomic ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04200">
        <property type="entry name" value="CuRO_2_ceruloplasmin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000009">
        <property type="entry name" value="Ceruloplasmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000002">
        <property type="entry name" value="Hephaestin like 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033138">
        <property type="entry name" value="Cu_oxidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002355">
        <property type="entry name" value="Cu_oxidase_Cu_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051553">
        <property type="entry name" value="Ran_GTPase-activating"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058923">
        <property type="entry name" value="RCC1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45982">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45982:SF1">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25390">
        <property type="entry name" value="WD40_RLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00633">
        <property type="entry name" value="RCCNDNSATION"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00079">
        <property type="entry name" value="MULTICOPPER_OXIDASE1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00080">
        <property type="entry name" value="MULTICOPPER_OXIDASE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00625">
        <property type="entry name" value="RCC1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00626">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="RCC1" evidence="16">
        <location>
            <begin position="37"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="RCC1-like" evidence="20">
        <location>
            <begin position="39"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="16">
        <location>
            <begin position="92"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="16">
        <location>
            <begin position="144"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="16">
        <location>
            <begin position="264"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="16">
        <location>
            <begin position="316"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="16">
        <location>
            <begin position="369"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="19">
        <location>
            <begin position="881"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="19">
        <location>
            <begin position="1229"/>
            <end position="1320"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="18">
        <location>
            <begin position="1386"/>
            <end position="1483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1485"/>
            <end position="1526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="17">
        <location>
            <begin position="1498"/>
            <end position="1510"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF25390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAJ8033737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1526" mass="170083" checksum="32764CBA9F2049FC" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BY04</accession>
    <name>A0A9Q1BY04_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF5641 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_22721</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BY04"/>
    <dbReference type="OrthoDB" id="10068969at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331:SF6">
        <property type="entry name" value="DOUBLECORTIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8035478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="217" mass="24526" checksum="BD78FB49189AAC9F" modified="2023-09-13" version="1">MDSSCFLNAIRFFEVVGLARRSDCGSNFVGACNEIGRQLGDKEPIKRYLLSHRCEWVFNPSHASNMGGAWERMIGIARMYVSISPDVKASKLIHEVFTTLMAEVAAIINSRPLLPDCNVPGVPLNLSPNMLLTMKSSQITAPEGVINHKDLLRSHWRRVQHLSNMFWEKWRKEYITSVTREVETPQEQSVSRGHFPLKARGPPKFLAFGKGSGSYHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BY42</accession>
    <name>A0A9Q1BY42_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_21942</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BY42"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8034915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="56" mass="6222" checksum="898A86E22A9904CD" modified="2023-09-13" version="1">MWLLANMGYQTNRIMPYIIHNGVMCSQSVILKTFGTDEGYLLLPSSSVRSSKPTGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BYW0</accession>
    <name>A0A9Q1BYW0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_22658</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BYW0"/>
    <dbReference type="OrthoDB" id="427030at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000155">
        <property type="entry name" value="zinc finger protein 133 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000870">
        <property type="entry name" value="zinc finger protein 197 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000624">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050636">
        <property type="entry name" value="C2H2-ZF_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47772:SF13">
        <property type="entry name" value="GASTRULA ZINC FINGER PROTEIN XLCGF49.1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47772">
        <property type="entry name" value="ZINC FINGER PROTEIN 200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="106"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="139"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="168"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="196"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="224"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="252"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="280"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="14"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ8035432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="388" mass="44646" checksum="9398A39343488CDB" modified="2023-09-13" version="1">MTNEISSGKKNRHSVKEDGLDSDKIEGKLKEVEDKTKDDKRFQCEFCEELFTSNRYRFKHRVASHPDCMFACPNCDRTFQVHEKDKWQHHVRIHNKPPKKPKQDSYSCHVCSMVFRAASSVAIHTQTFHPENVKVEKPFCCEVCGKLFRTKRAIKLHQTLVHSDKKPYECPTCGRGFKMESYLKLHLRTHTGEKPFACKFCDKTFPSTAGRRSHERTHTNERPYKCSYCDRRFKAKPQLVEHVRTHTKEKPFVCPVCGKAFASRTSFATHKFLHTGKKPFQCSSCSRAFAQKCNLEHHVRSIHTQEKPHQCNICGVRFSRSTGRSMHMEKKHGIKVSLKQGRPLIYPEAQEMDQIVQAGCVSIDVSSGIMGPTMLEDTLSLVVESLNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BZ22</accession>
    <name>A0A9Q1BZ22_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Titin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_22382</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BZ22"/>
    <dbReference type="OrthoDB" id="428111at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030017">
        <property type="term" value="C:sarcomere"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000345">
        <property type="entry name" value="Muscle M-line assembly protein unc-89"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47633">
        <property type="entry name" value="IMMUNOGLOBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47633:SF4">
        <property type="entry name" value="MYOPALLADIN ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8035225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="193" mass="20700" checksum="0D45F80301926998" modified="2023-09-13" version="1">MTIIQGEPLQLKCSFHGYPIPTILWYKDGTQLDPEAGYKTTSCEGVAMLTSQSTKEGDEGMFSCVAVNPEGDDITSCNVLVEISTVMVQPSDDNCTLAEVRSDVTDKGLPCANPDESGKTAVDTPACKHDENLTVGFHEEDLSTSPKPPLDPLNGEVTKDVADTVALPLRIKCLKCGNSLCTIEVTGGEWCEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BZ94</accession>
    <name>A0A9Q1BZ94_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_19430</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8035681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BZ94"/>
    <dbReference type="OrthoDB" id="6747990at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35450">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35450:SF2">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8035681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="120" mass="13911" checksum="82224A83B2AC3BAE" modified="2023-09-13" version="1">MCREVDETVSHIVAGCQTLAGTEYLKKHDSVAAALHLEICRHYRIPTTERQPWLHKPEAVTETEQVKVLWYFEIKTDRIVTARRPDLIVIDKQEKTAKIIDVAFPLDKNVRDEEVQKGQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C1W1</accession>
    <name>A0A9Q1C1W1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Polycystic kidney disease protein 1-like 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HOLleu_18489</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Nanhai2018</strain>
            <tissue evidence="18">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the polycystin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="444119at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050982">
        <property type="term" value="P:detection of mechanical stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00146">
        <property type="entry name" value="PKD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01752">
        <property type="entry name" value="PLAT_polycystin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000086">
        <property type="entry name" value="Polycystic kidney disease 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.60.20:FF:000022">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.60.20">
        <property type="entry name" value="PLAT/LH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000203">
        <property type="entry name" value="GPS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002859">
        <property type="entry name" value="PKD/REJ-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013122">
        <property type="entry name" value="PKD1_2_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003915">
        <property type="entry name" value="PKD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000601">
        <property type="entry name" value="PKD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035986">
        <property type="entry name" value="PKD_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001024">
        <property type="entry name" value="PLAT/LH2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036392">
        <property type="entry name" value="PLAT/LH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042060">
        <property type="entry name" value="PLAT_polycystin1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051223">
        <property type="entry name" value="Polycystin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046791">
        <property type="entry name" value="Polycystin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10877">
        <property type="entry name" value="POLYCYSTIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10877:SF150">
        <property type="entry name" value="REJ DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01825">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00801">
        <property type="entry name" value="PKD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08016">
        <property type="entry name" value="PKD_channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01477">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20519">
        <property type="entry name" value="Polycystin_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02010">
        <property type="entry name" value="REJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01433">
        <property type="entry name" value="POLYCYSTIN2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00303">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00308">
        <property type="entry name" value="LH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49723">
        <property type="entry name" value="Lipase/lipooxygenase domain (PLAT/LH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49299">
        <property type="entry name" value="PKD domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50093">
        <property type="entry name" value="PKD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50095">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0969">Cilium</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1793"/>
            <end position="1813"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="2003"/>
            <end position="2023"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="2043"/>
            <end position="2066"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="2174"/>
            <end position="2198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="2218"/>
            <end position="2241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="2309"/>
            <end position="2329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="2565"/>
            <end position="2587"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="2599"/>
            <end position="2621"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="2653"/>
            <end position="2673"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="2693"/>
            <end position="2715"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="2755"/>
            <end position="2776"/>
        </location>
    </feature>
    <feature type="domain" description="PKD" evidence="16">
        <location>
            <begin position="318"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="PKD" evidence="16">
        <location>
            <begin position="546"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="domain" description="PLAT" evidence="17">
        <location>
            <begin position="1838"/>
            <end position="1957"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1404"/>
            <end position="1427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1406"/>
            <end position="1418"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="2417"/>
            <end position="2430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR603915-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ8037623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="2878" mass="323803" checksum="82191F041E7C7D89" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C5Q5</accession>
    <name>A0A9Q1C5Q5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Solute carrier family 35 member G1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_16744</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8039122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C5Q5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22911">
        <property type="entry name" value="ACYL-MALONYL CONDENSING ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="66"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="97"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="128"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="159"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="182"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="219"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="247"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="280"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="311"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="334"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="3">
        <location>
            <begin position="68"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="3">
        <location>
            <begin position="218"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8039122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="360" mass="38897" checksum="80BFCB8FC2E19D63" modified="2023-09-13" version="1">MKKNANILAQDRTHPNDRRSDQERRLLISKDGKGSEGKWVSKNTENNSVVRGLQSWLKGISSGSKIQGIFIAISFTFFMATRNVFAQLSTKGLSPFAVAAMDRIVTGLCCVPYLIIQKISPRVSAADTFVLVIRGVCSAVVLSSLIAALEKLPVGDASAIQSLASIPAGIWGALFLGERFGLVDFMCALLGALGFSLISKPDFIFNMDTGELSEHLEGYGLALLSAFSLSVIFPISRFLLKSKIDPVLIVFWLSLTSATLTGALATFMGQWSNVWCNPSLVYVILMGGCAFFANVLIVVSLSILDTFEVAILNQNVIIFAYMLQVTLLSVSPSWLSLLGAAMIILACIITTIHKSINARS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C842</accession>
    <name>A0A9Q1C842_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Myb-like, SWIRM and MPN domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_14304</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M67A family. MYSM1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C842"/>
    <dbReference type="OrthoDB" id="7464992at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000555">
        <property type="entry name" value="JAMM/MPN+_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050242">
        <property type="entry name" value="JAMM_MPN+_peptidase_M67A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007526">
        <property type="entry name" value="SWIRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10410">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01398">
        <property type="entry name" value="JAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04433">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102712">
        <property type="entry name" value="JAB1/MPN domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50934">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb-like" evidence="6">
        <location>
            <begin position="94"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="9">
        <location>
            <begin position="97"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="10">
        <location>
            <begin position="101"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="SWIRM" evidence="8">
        <location>
            <begin position="377"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="domain" description="SWIRM" evidence="8">
        <location>
            <begin position="464"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="domain" description="MPN" evidence="7">
        <location>
            <begin position="643"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="177"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="565"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="181"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="214"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="600"/>
            <end position="612"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00032256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8040102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="890" mass="101194" checksum="2AB31676823E6C6D" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CBV8</accession>
    <name>A0A9Q1CBV8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Sedoheptulokinase</fullName>
            <ecNumber evidence="10">2.7.1.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Carbohydrate kinase-like protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_09670</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Acts as a modulator of macrophage activation through control of glucose metabolism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>sedoheptulose + ATP = D-sedoheptulose 7-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23844"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16802"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57483"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.14"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FGGY kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.14" evidence="10"/>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8042813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CBV8"/>
    <dbReference type="OrthoDB" id="10264182at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050277">
        <property type="term" value="F:sedoheptulokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901135">
        <property type="term" value="P:carbohydrate derivative metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071222">
        <property type="term" value="P:cellular response to lipopolysaccharide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006091">
        <property type="term" value="P:generation of precursor metabolites and energy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006071">
        <property type="term" value="P:glycerol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046496">
        <property type="term" value="P:nicotinamide nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006163">
        <property type="term" value="P:purine nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07777">
        <property type="entry name" value="ASKHA_NBD_FGGY_SHK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000111">
        <property type="entry name" value="Sedoheptulokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000132">
        <property type="entry name" value="Sedoheptulokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018484">
        <property type="entry name" value="FGGY_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10196:SF67">
        <property type="entry name" value="SEDOHEPTULOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10196">
        <property type="entry name" value="SUGAR KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00370">
        <property type="entry name" value="FGGY_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbohydrate kinase FGGY N-terminal" evidence="13">
        <location>
            <begin position="9"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00052736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00057196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00066341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00069425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00076706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ8042813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="465" mass="51024" checksum="46FC46299A244161" modified="2023-09-13" version="1">MASSGGDLCLGIDIGTTSVKLSLIGQDDTQAHEQILKSSDSVMPSISAPKSGSEQNIWLLFQTIQESVNKLDALKLKRVTKIAVTGQMHGCVLWKAKSVRITEGKLTVSSNDVSPLFNWQDGRCSSDFLATLPKPKSHLRIATGHGCATLFWWSRNYPGYLEQFDYAGTVMDLFVSALCGQDEPVMSVQNAASWGYFDCESSTWNKEILSEAGFPIRFLPKVKESGSLAGSLQSFWLDLQSGTKVSVAMGDLACSVYSVLQTPTDAAMNVGTSAQLVRGMPDGFVPPMDSHTSPVEYFPYFDNRYLAVAAALNGGNALSTFVDMLMQWFSPLSTGVIPTKDEVYKKLTNLANKAETDLVVDPVFLGERHRQDDRASIFNIRPDNADLGGLFRAFCRGIIQNMASMMPREELKSGSVQRIVGCGSALNRNPVLKEELERCFELPVMYREDCDACFGAALYALRADW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CCA7</accession>
    <name>A0A9Q1CCA7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_12885</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CCA7"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8041944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="72" mass="8173" checksum="AC74CCE008BE1B03" modified="2023-09-13" version="1">MCSNLYKIKLHHTCLTVSKNMFHLNNLDHPSVISQTSAPNQETPFLLVKTAFISLLPKLGMSWQVRLDLPPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CCK8</accession>
    <name>A0A9Q1CCK8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nuclear transport factor 2</fullName>
            <shortName evidence="2">NTF-2</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_13258</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Has a role in nuclear-cytoplasmic transport of proteins and mRNAs.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8042248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CCK8"/>
    <dbReference type="OrthoDB" id="6507044at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00780">
        <property type="entry name" value="NTF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.50:FF:000005">
        <property type="entry name" value="Nuclear transport factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045875">
        <property type="entry name" value="NTF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002075">
        <property type="entry name" value="NTF2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018222">
        <property type="entry name" value="Nuclear_transport_factor_2_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12612">
        <property type="entry name" value="NUCLEAR TRANSPORT FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02136">
        <property type="entry name" value="NTF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50177">
        <property type="entry name" value="NTF2_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0653">Protein transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="NTF2" evidence="3">
        <location>
            <begin position="6"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU369002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8042248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="124" mass="14203" checksum="3FC4C19DECC65AE1" modified="2023-09-13" version="1">MDPSQIANVFVKQYYDTFDKNRPDVVGLYHPQTCVLTFEGQVCSGKEEIMKKLTSLAFTNIQHVITTVDAQPTYDDMIVVFVVGQLKTDEDNPHSFSQTFVLKKEADNRLYVVNDMFRLSLHHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CDM3</accession>
    <name>A0A9Q1CDM3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_09644</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8042788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CDM3"/>
    <dbReference type="OrthoDB" id="1607513at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052035">
        <property type="entry name" value="ZnF_BED_domain_contain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46481:SF10">
        <property type="entry name" value="ZINC FINGER BED DOMAIN-CONTAINING PROTEIN 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46481">
        <property type="entry name" value="ZINC FINGER BED DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8042788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="105" mass="11247" checksum="3C80712398A527E2" modified="2023-09-13" version="1">MGTNGKDEGVWHTAAALTQQMSAIVEEWALEKKLGTIVHDNASNIKNIGRSLGGDDMPCDGHTLQLCINNGLKAHANIGRIISCGRRLAGHFKRSVVATKALTLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CFP3</accession>
    <name>A0A9Q1CFP3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_11135</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CFP3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040401474" evidence="1">
        <location>
            <begin position="22"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8043853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="105" mass="11495" checksum="EA4950B2BF2B648A" modified="2023-09-13" version="1">MFSSRVLILLVLFGAVAIIASSPAEESSDEEELDLSERGLINRFCEKRLHRHHKRGLQEDEPSDEDIDEMVARVAKLNPGSRSWGSFFGNHAAPLAAAALHAAGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CH36</accession>
    <name>A0A9Q1CH36_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Enoyl-CoA delta isomerase 1, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_08175</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CH36"/>
    <dbReference type="OrthoDB" id="1696280at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8045217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="128" mass="14804" checksum="A95824348B5FAD7C" modified="2023-09-13" version="1">MWLWTHLGRGVYVPFYLRQMLENLIGPKATARAVQLSLVFTSAEACKIGWVEEVTPLDQVMTAAKRELKQWLEIPDSGRTLMKMDIRRKEIQEIIDRREEDAEAFASLISRPGIQEQFIVSINKSKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CH73</accession>
    <name>A0A9Q1CH73_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Prospero homeobox protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_07787</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CH73"/>
    <dbReference type="OrthoDB" id="10038576at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048468">
        <property type="term" value="P:cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.500:FF:000005">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.500">
        <property type="entry name" value="Homeo-prospero domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023082">
        <property type="entry name" value="Homeo_prospero_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037131">
        <property type="entry name" value="Homeo_prospero_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039350">
        <property type="entry name" value="Prospero_homeodomain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12198:SF0">
        <property type="entry name" value="HOMEOBOX PROTEIN PROSPERO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12198">
        <property type="entry name" value="HOMEOBOX PROTEIN PROSPERO/PROX-1/CEH-26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05044">
        <property type="entry name" value="HPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51818">
        <property type="entry name" value="HOMEO_PROSPERO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 9" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Prospero" evidence="8">
        <location>
            <begin position="463"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="29"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="100"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="260"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="413"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="100"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="134"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="295"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="322"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="431"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8044901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="622" mass="69273" checksum="8F22703FC4FBAE50" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CI01</accession>
    <name>A0A9Q1CI01_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Leucine-rich repeat-containing protein 57</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_08718</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CI01"/>
    <dbReference type="OrthoDB" id="1728874at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000230">
        <property type="entry name" value="Leucine-rich repeat-containing protein 57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF62">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8045671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="237" mass="26506" checksum="E4AC8F919F90247D" modified="2023-09-13" version="1">MGNSIKPHIERAEKSGVCQLSNQGLSEFPPQLMKLVKNLRTLDISHNKIASIPDGIKNFDQLKSFSADHNRITLISEALCSLRKLETLSISHNKLSNISSAVGQLRAIKTINLSSNQLTHFPLELCQLKHLDVLDLSQNAIRELPSGIGDLQAMEVNLNQNQISILPADIARCPRLKVLRLEENCLQLEAIPQEVLSNSQISLLALEGNLFAMKEFVNCPGYDEYMERYTAAKKKMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CIM0</accession>
    <name>A0A9Q1CIM0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">5-hydroxytryptamine receptor 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_08117</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CIM0"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00637">
        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050569">
        <property type="entry name" value="TAAR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24249">
        <property type="entry name" value="HISTAMINE RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="15"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="89"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="189"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="10">
        <location>
            <begin position="36"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8045169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="332" mass="36810" checksum="FB748141CAC96C04" modified="2023-09-13" version="1">MINSTATANTQDAFLAFHVVTHLVMANANGVLCILFNSLNLVTLRRETSCFGEATTVFVKCLALTDLLTGVCIILAADLTMWKQLVTHFSFFCSLTFWSTSFITLLSFHFISCLTFDRVVAIAFPLRHRALLPPERARKISFCAFINVLAISVFISFDKFGISNHYFDVQGHKCISEGFYKFSAMTWSYYIYASIVGLFIPFSFTVGANIYLFIVTVKHAAQINVAVPENVLPRDKRPVKISVVVPQNAVQLQHAAGENAEHASQCNVSPENALPPTLSIGARNPAFAVSVEATETRTPKSDGTTHNRKKTFVRELRVVRTFLIITHSSGIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CJB5</accession>
    <name>A0A9Q1CJB5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Helicase ARIP4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_05009</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="2020972at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18069">
        <property type="entry name" value="DEXHc_ARIP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044574">
        <property type="entry name" value="ARIP4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044573">
        <property type="entry name" value="ARIP4_DEXHc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45797:SF1">
        <property type="entry name" value="HELICASE ARIP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45797">
        <property type="entry name" value="RAD54-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 12" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="321"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="751"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="204"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1153"/>
            <end position="1215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1371"/>
            <end position="1444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="30"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="76"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="86"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="106"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="150"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="233"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="260"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1159"/>
            <end position="1175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1197"/>
            <end position="1210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1430"/>
            <end position="1444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8046361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1461" mass="162486" checksum="53DD444E5488D3B1" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CK24</accession>
    <name>A0A9Q1CK24_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">small monomeric GTPase</fullName>
            <ecNumber evidence="1">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_05446</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="1"/>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CK24"/>
    <dbReference type="OrthoDB" id="5976022at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032486">
        <property type="term" value="P:Rap protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04175">
        <property type="entry name" value="Rap1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000182">
        <property type="entry name" value="ras-related protein Rap-1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038851">
        <property type="entry name" value="Rap1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020849">
        <property type="entry name" value="Small_GTPase_Ras-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24070">
        <property type="entry name" value="RAS, DI-RAS, AND RHEB FAMILY MEMBERS OF SMALL GTPASE SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="169"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="175"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8046683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="188" mass="21146" checksum="882271E7BCA69185" modified="2023-09-13" version="1">MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEVEGQQCMLEILDTAGTEQFTAMRDLYMKNGQGFVLVYSITAQSTFNDLHDLREQILRVKDTDNVPMVLVGNKCDLEDERVVGKDQGSNLAKQFCNCTFLETSAKTKINVNEIFYDLVRQINRQTPDTKGKKGKSGKKRGCVIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CLD7</accession>
    <name>A0A9Q1CLD7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Solute carrier family 13 member 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_06536</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC13A/DASS transporter (TC 2.A.47) family. NADC subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CLD7"/>
    <dbReference type="OrthoDB" id="6493944at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015556">
        <property type="term" value="F:C4-dicarboxylate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005310">
        <property type="term" value="F:dicarboxylic acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001898">
        <property type="entry name" value="SLC13A/DASS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10283">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 13 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10283:SF82">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 13 MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00939">
        <property type="entry name" value="Na_sulph_symp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="18"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="41"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="86"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8047512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="127" mass="14928" checksum="A83355AF9F87DC33" modified="2023-09-13" version="1">MEVKTKHRLRYWLWNRQYYLVPIIPILCCWVPIVYNNSIGYTTYIIIIMALYWCLELIPLAATSLLPMVLFPLFGVLKSDDVCKQYLKDTHVMIFGGLMVAIAIERWNLHRRIALKVLLMVGTKPAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CN55</accession>
    <name>A0A9Q1CN55_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein 243</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_06806</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CN55"/>
    <dbReference type="OrthoDB" id="17800at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022564">
        <property type="entry name" value="DUF2678"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28603">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 243"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28603:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 243"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10856">
        <property type="entry name" value="DUF2678"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8047735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="117" mass="13604" checksum="E80A01C6A4DB259C" modified="2023-09-13" version="1">MSSRLYDGGDVQTPLFGEQQPRDRVINIVISAVAVFIVMSTLVSAFFCPWPPCWQNVTFSFVVLMVCSGNLILVYWYRQGDVDPKFRRLIYFNTFTIMLLSICAMFYIFKDKCDRKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CNG1</accession>
    <name>A0A9Q1CNG1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_00787</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CNG1"/>
    <dbReference type="OrthoDB" id="4327074at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15489">
        <property type="entry name" value="PHD_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="5">
        <location>
            <begin position="238"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="68"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="194"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="279"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="87"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="143"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8048461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="301" mass="32948" checksum="22534E27B5A212D1" modified="2023-09-13" version="1">MRDNPGKPITRYDISRLFTIAYHSGATMGNLVNGFKYTGIFPLDAKAIPDHAYALAETTEQAKTIHLQEGAPPKSSENDDSVAPAETTGQATSNVLQESEPAQSSENDGRISNEDTDLEVAMPSIRPEMSTESSSNLPCEDQGSTSSDTTSLGGRVSFSDILPLPKAAPVKRKRSHKSLIGLVTSADVIERLEKEKKQKESVPKSQKNAQLKKSKGKLPAAKKVKCKASAEQDPDNFCGYCNDYYFDMDDEDEDWLYCKRCLKWYHESCFGLMVPVSEVPSGNDVESSMDERDGSTDGNDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CNV4</accession>
    <name>A0A9Q1CNV4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_00268</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CNV4"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8048095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="534" mass="60873" checksum="9B138D54BF309CF6" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CQ53</accession>
    <name>A0A9Q1CQ53_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Dehydrogenase/reductase SDR family member 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_01573</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CQ53"/>
    <dbReference type="OrthoDB" id="3592703at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004090">
        <property type="term" value="F:carbonyl reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43943">
        <property type="entry name" value="DEHYDROGENASE/REDUCTASE (SDR FAMILY) MEMBER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43943:SF2">
        <property type="entry name" value="DEHYDROGENASE_REDUCTASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8049026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="194" mass="21520" checksum="0285F7F9925D410D" modified="2023-09-13" version="1">MEVFTNNYGSQPIQSSKMPLERFLGRVAILTGSTQGIGLATARRLGQEGCKVIISSRRQENVDKAVKHLTKEGIEACGITANQRIKEDRQKFLKLASEKYGGFDHVFISAGVSPHRGSLLTVTEEQFDRIFDTNLKSNFQFIQECVPYMEKRGGGSIVTNSTAGVYGVTAAVIGKVCKYCIVKLQQMCISKRYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CQR3</accession>
    <name>A0A9Q1CQR3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Sushi domain-containing protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_02717</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CQR3"/>
    <dbReference type="OrthoDB" id="6127264at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005533">
        <property type="entry name" value="AMOP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056619">
        <property type="entry name" value="C8-3_MUC4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051495">
        <property type="entry name" value="Epithelial_Barrier/Signaling"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001212">
        <property type="entry name" value="Somatomedin_B_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001846">
        <property type="entry name" value="VWF_type-D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13802">
        <property type="entry name" value="MUCIN 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13802:SF59">
        <property type="entry name" value="SUSHI DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03782">
        <property type="entry name" value="AMOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23263">
        <property type="entry name" value="C8-3_MUC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50856">
        <property type="entry name" value="AMOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00524">
        <property type="entry name" value="SMB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50958">
        <property type="entry name" value="SMB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51233">
        <property type="entry name" value="VWFD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0768">Sushi</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="SMB" evidence="12">
        <location>
            <begin position="76"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="AMOP" evidence="10">
        <location>
            <begin position="209"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="VWFD" evidence="13">
        <location>
            <begin position="385"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="11">
        <location>
            <begin position="659"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="8">
        <location>
            <begin position="738"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="9">
        <location>
            <begin position="902"/>
            <end position="1168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ8049807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1241" mass="135415" checksum="63A06028C7851EF8" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CSW4</accession>
    <name>A0A9Q1CSW4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Angiopoietin-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_03688</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CSW4"/>
    <dbReference type="OrthoDB" id="6236007at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19941">
        <property type="entry name" value="TIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036084">
        <property type="entry name" value="Ser_inhib-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040941">
        <property type="entry name" value="GGGWT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57567">
        <property type="entry name" value="Serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="350"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="3">
        <location>
            <begin position="281"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8050470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="390" mass="42657" checksum="260EBE954C0C59F3" modified="2023-09-13" version="1">MLNFLCLATGPSIQGQGFETIYPPFHHTAVFFGTIKGRKPVPYHRFVTKTLHPAPPVRRHLHPGGQGALRRNKRVQPNGVIKGSSTSMPIFNDTERTSTTDLSTVDHSTHVPFSSEQHMLNTLSTNQVVQTYEPVLRISTTYATTKQGSGNGATTEYPISTTIPCPPNMVYAYCTEGQGTCQNPSGVTVTSCSVPMTCVCPPGCLMKDNACVPREECECFIAGAGVLLDGETNVNADCTNRCECQSNVLTCDYSYSSAATCEERGGVCQCYCDDGFKGNGQNCEPDATDCADVYNAGFRQSGVYTIKPTNWPGSPFEAYCNMTHGGGGRYRVTHHLMKFKVSLVKTLTAFLNYIMFMFVYVHGEVSSFYLLFSKFNLSFNVKNLDCSCGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CSX5</accession>
    <name>A0A9Q1CSX5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_04139</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CSX5"/>
    <dbReference type="OrthoDB" id="5989452at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="14"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8050801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="244" mass="28412" checksum="9CCD47B1A66915D2" modified="2023-09-13" version="1">MIFSALFYRRKKRGKRQQSISKENATTPSGFESYEEPQERRTNFQTYVHFMPLPALPDKKRESIEDENVYYSDENESFTNRRVFQQRDLSFVLRLNSGSIYDRWIITPSKQNKSRLSTYFVASTVTVGNAAQKQITHWNDFAKRILEIPDVEFVVKTRGICIDGGCLYLIQDHIMCQTLEKYQRTKEGSGQLPSVSEFEIMRFINDILKGMEFIHSYGIMTLAKISMNDIDLSNDYMPMGGNET</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-04-02" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CTX0</accession>
    <name>A0A9Q1CTX0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BRCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_03851</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="5997366at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRCT" evidence="2">
        <location>
            <begin position="1208"/>
            <end position="1309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="296"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="344"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="300"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="344"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="383"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8050589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1453" mass="165761" checksum="1728E27FA84E78BB" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CUB9</accession>
    <name>A0A9Q1CUB9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Microfibrillar-associated protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_04213</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MFAP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CUB9"/>
    <dbReference type="OrthoDB" id="1111734at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033194">
        <property type="entry name" value="MFAP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009730">
        <property type="entry name" value="MFAP1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15327">
        <property type="entry name" value="MICROFIBRIL-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06991">
        <property type="entry name" value="MFAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Micro-fibrillar-associated protein 1 C-terminal" evidence="3">
        <location>
            <begin position="212"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="37"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="268"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="312"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="412"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="71"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="109"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="121"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="148"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="162"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="195"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="226"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="268"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="290"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="312"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="420"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF06991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8050854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="462" mass="54382" checksum="E41A0FEA2000E767" modified="2023-09-13" version="1">MDIDIVPEPGGLIIQSTAGAVPVANKKGEMTMQKVKVKRYVPGKRPDYAPESSSSEDEDELPMARAKRTAGRFEEDARLRRLRERHRDADDEEEEDRHTRHRQIHEPEILEDGEVSEAEDETRRVEEEDTNQDRGSRSSRKVFRIEADESSSEEEDLEEEEIEKRRAALRARALEMRQQEEEDLMGQEDEKPKGDEEESEEESSEYEDYSDSEEETGPRLKPVFVPKRDRVTIQEKEQAAQKIRQQEEMAKRRIEDRKRETLRMVETEARKAYEGDEKPVTEASLAAVNTDDENGEEEYESWKVRELKRIKRDREEREQEEKEKQELERIRNLTDEQRKEEERRNPRVVTNQAKKGRYKFLQKYYHRGAFFMDEEQDVFKRDVSNPTLEDHFDKKVLPKVMQVKNFGRSGRTKYTHLADQDTSQTDSPWVSDNSLSRKFQAKIAGGLKQSFEKPTAKKHGEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1GZ57</accession>
    <name>A0A9Q1GZ57_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_30157</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1GZ57"/>
    <dbReference type="OrthoDB" id="9894375at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="126"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="160"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="7">
        <location>
            <begin position="120"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="39"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="68"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8028033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="224" mass="25412" checksum="08B93CBC87AA18FF" modified="2023-09-13" version="1">MLLRKDSREPQGLVEYCETNQGSDCPHDSDTESMEDSDDHTIADEPPVKEDNPNSNEKPVRRHIHDISSMQKPATSSRVAQATDRGSSMRNDTMHHGHVNQPTHRLGDGPRRHIDGRIMQEARSALRLFFVVAVYIINWVPGILFVLTTVVVSERNISPYLYVVMHTLLYTSVIFAPYIYGWKQNGSQGGKNHAPPEVGKNIDGQCRQHSYLCQHQTHKAWTCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1GZ68</accession>
    <name>A0A9Q1GZ68_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_30175</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1GZ68"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8028048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="110" mass="12754" checksum="BCE6A1EDEECC36BE" modified="2023-09-13" version="1">MSPIYMHNTHTHTLMHRPMMCVLLIGSQHVKWGKRQKCSNLLRHIFERCKIQFHGKFCIRELGISPLAGVSPLARGKKRSCPRFTSFTYAHTHTYTHAHTHIHTHAIMTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1GZM0</accession>
    <name>A0A9Q1GZM0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_29774</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8027735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1GZM0"/>
    <dbReference type="OrthoDB" id="5983107at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8027735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="93" mass="10503" checksum="C17496351FA856FA" modified="2023-09-13" version="1">MSELPAAPWTEVSIDFSGPFPSGDYLLVITDDYSRYPIVEITKSTSAKASFLILDNIFHLFESHKWSKQITDHLSKDMSFVNLLSTLVFDTGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1GZQ4</accession>
    <name>A0A9Q1GZQ4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Nuclear cap-binding protein subunit 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_29540</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1GZQ4"/>
    <dbReference type="OrthoDB" id="10252707at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005846">
        <property type="term" value="C:nuclear cap binding complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000339">
        <property type="term" value="F:RNA cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050684">
        <property type="term" value="P:regulation of mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027159">
        <property type="entry name" value="CBP80"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015172">
        <property type="entry name" value="MIF4G-like_typ-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015174">
        <property type="entry name" value="MIF4G-like_typ-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12412">
        <property type="entry name" value="CAP BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12412:SF2">
        <property type="entry name" value="NUCLEAR CAP-BINDING PROTEIN SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09088">
        <property type="entry name" value="MIF4G_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09090">
        <property type="entry name" value="MIF4G_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MIF4G-like type 1" evidence="2">
        <location>
            <begin position="51"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="MIF4G-like type 2" evidence="3">
        <location>
            <begin position="180"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="330"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF09090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8029982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="473" mass="54873" checksum="49EE03D143FE23C5" modified="2023-09-13" version="1">MKAHCANKGPVLPASTSINRYLVEDCLYRIVHVYLDRKDCAAQLVGFHAKNRFPYHYMIVEVVFGSLFSLPSTKNIEVFYASLLLELCKLQPGFLPQVLAQATEMLYERVEGMNLTCMERFIRWFSHHLSNFQFRWSWDEWIDCTEKEPGHAKVIFIQEVLQRCLRGTGWKDEGGGDQIPALPISKKLIEAFKAKASVEQVEEILKQIPGPKKDDDSGEAEEAVSPLKIDVFVQSLLFLGQKSFSHTFSALAKFHSLLKKLGSNEESQIFMLRTIRDLWQNHQQMICVIIDKLIRTQIVSCPAVINWIFSEHMRQHFTEGFVWEILHGVLKKMNLEVLKLREELEEAREKKSKREEGEASGDEYDISNTTTESQIEKLQESYEAAQSEQKNLFLIIFQRFIILLTEHIISCESKGTSFQTNWFIHSIHRLEQIFILHFKQVVKYTSTLENLLFTSDTDPNLLKVFHQFCSLRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H0D1</accession>
    <name>A0A9Q1H0D1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_32830</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H0D1"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8027631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="82" mass="9366" checksum="6E4F2E9ABCB8064D" modified="2023-09-13" version="1">MCDVKVAKAMSGAECWKTTKRLTVSKLTDRKITDDFSRDLDAKMSELPTVPINIKEQWASVRDTVYSVALEYFGRAKRKHQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H1F5</accession>
    <name>A0A9Q1H1F5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Nose resistant to fluoxetine protein 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_24765</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8031542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H1F5"/>
    <dbReference type="OrthoDB" id="118951at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002656">
        <property type="entry name" value="Acyl_transf_3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006621">
        <property type="entry name" value="Nose-resist-to-fluoxetine_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052728">
        <property type="entry name" value="O2_lipid_transport_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11161">
        <property type="entry name" value="O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11161:SF0">
        <property type="entry name" value="O-ACYLTRANSFERASE LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01757">
        <property type="entry name" value="Acyl_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20146">
        <property type="entry name" value="NRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00703">
        <property type="entry name" value="NRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040274256" evidence="2">
        <location>
            <begin position="19"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="166"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="269"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="310"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="359"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="421"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="447"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="493"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="525"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="589"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="630"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="656"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="domain" description="Nose resistant-to-fluoxetine protein N-terminal" evidence="3">
        <location>
            <begin position="39"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8031542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="693" mass="79563" checksum="72713BCF996FFFDB" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H1V0</accession>
    <name>A0A9Q1H1V0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">LINE-1 reverse transcriptase-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_26825</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H1V0"/>
    <dbReference type="OrthoDB" id="8961218at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="1">
        <location>
            <begin position="7"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8030404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="115" mass="13286" checksum="F38F4FA17871DB78" modified="2023-09-13" version="1">MNELNIISYNARGLQGNKKRRRLFSYFHRRKVDVIVIQETHSLQTDELFWKNKWGGNAYFSHGTNDSCGVCILFKPSLNFDVVKLKPHNLGRYILLDILVALVGVYSPNSDNPNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H2H7</accession>
    <name>A0A9Q1H2H7_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Fructose-1,6-bisphosphatase isozyme 2</fullName>
            <ecNumber evidence="8">3.1.3.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">D-fructose-1,6-bisphosphate 1-phosphohydrolase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">D-fructose-1,6-bisphosphate 1-phosphohydrolase 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">Muscle FBPase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="ORF">HOLleu_29219</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="27">Nanhai2018</strain>
            <tissue evidence="27">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="19">Catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate in the presence of divalent cations and probably participates in glycogen synthesis from carbohydrate precursors, such as lactate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>beta-D-fructose 1,6-bisphosphate + H2O = beta-D-fructose 6-phosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:11064"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="EC" id="3.1.3.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="6">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="20">Homotetramer. Interacts with ALDOA; the interaction blocks inhibition by physiological concentrations of AMP and reduces inhibition by Ca(2+). Interacts with alpha-actinin and F-actin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Myofibril</location>
            <location evidence="4">Sarcomere</location>
            <location evidence="4">Z line</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 24">Belongs to the FBPase class 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="27">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.11" evidence="8"/>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H2H7"/>
    <dbReference type="OrthoDB" id="10256725at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070161">
        <property type="term" value="C:anchoring junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042132">
        <property type="term" value="F:fructose 1,6-bisphosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030388">
        <property type="term" value="P:fructose 1,6-bisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006000">
        <property type="term" value="P:fructose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005986">
        <property type="term" value="P:sucrose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00354">
        <property type="entry name" value="FBPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.80:FF:000001">
        <property type="entry name" value="Fructose-1,6-bisphosphatase class 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.540.10:FF:000005">
        <property type="entry name" value="Fructose-1,6-bisphosphatase isozyme 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.540.10">
        <property type="entry name" value="Fructose-1,6-Bisphosphatase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01855">
        <property type="entry name" value="FBPase_class1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044015">
        <property type="entry name" value="FBPase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000146">
        <property type="entry name" value="FBPase_class-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033391">
        <property type="entry name" value="FBPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028343">
        <property type="entry name" value="FBPtase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006778">
        <property type="entry name" value="PRK09293.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11556">
        <property type="entry name" value="FRUCTOSE-1,6-BISPHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11556:SF1">
        <property type="entry name" value="FRUCTOSE-BISPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00316">
        <property type="entry name" value="FBPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18913">
        <property type="entry name" value="FBPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500210">
        <property type="entry name" value="FBPtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000904">
        <property type="entry name" value="FBPtase_SBPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00115">
        <property type="entry name" value="F16BPHPHTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56655">
        <property type="entry name" value="Carbohydrate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0106">Calcium</keyword>
    <keyword evidence="17 24" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="15" id="KW-0965">Cell junction</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12 24" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-0460">Magnesium</keyword>
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            <dbReference type="Proteomes" id="UP001152320"/>
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    <sequence length="336" mass="36433" checksum="A2DF94FA269C5AD1" modified="2023-09-13" version="1">MAGIDTDCMTLSRFMLEEQGRCEGATGEMTQLLNAISTACKGISSAVHRAGIAQLYGIAGSMNTTGDQQKKLDVLSNDLFVNQLRSSFATCALVSEENETLVEVETAKQGKYIVAFDPLDGSSNIDCLVSIGSVFGIWKRKNAKGPVATEEALQKGRDMVAAGYCLFGSATFIVVSTGCGVNGFQLDPTIGEFILTDKIMSVKKRGKIISCNEGYAKYWDPAITEYVNKKKFPEGDIPPYSCRYIGSMVADVHRTIKYGGIFLYPGNSKSPEGKLRLQYECNPMAFLIEQAGGIATTGTQPVLDVQPTDIHQRCPMILGSPDDVNEFLEVAAKHKK</sequence>
</entry>
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    <accession>A0A9Q1H2I6</accession>
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        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
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        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
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                <person name="Chen T."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
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    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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            <dbReference type="Proteomes" id="UP001152320"/>
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</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
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        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
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        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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    </evidence>
    <sequence length="558" mass="61400" checksum="A5AAE19850CD6281" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H3Z8</accession>
    <name>A0A9Q1H3Z8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_26100</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H3Z8"/>
    <dbReference type="OrthoDB" id="6428524at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="430"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="430"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="449"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8032549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="595" mass="68879" checksum="3EA8A7430101BE32" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H4L0</accession>
    <name>A0A9Q1H4L0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_25523</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000012">
        <property type="protein sequence ID" value="KAJ8032101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H4L0"/>
    <dbReference type="OrthoDB" id="5986940at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8032101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="338" mass="36565" checksum="BD5078C867D10600" modified="2023-09-13" version="1">MATTFTRKFYKSLFRNVIQICSEYTSFDSPVSDSDGDVVRCRYATGFSECGSVCSGLPGATLDQDTCSFQWTPTVQGLYAVAMQIEDYLDSSSTTPLSSVSLQYILDVRSLGPCSNGPSFASDVLPGGSCVAVSQGQMFTTRITASVADPSQSITSVNTISPSGLTMSPVQPVPGSPTDYFFDVQFTASPQSRERELFCFAVMTNIGFPSEQRCITFATNVPVPLPLAASVDRNNIVTVTIDRMGSRADTSRSVRIVYAANQRLVYTFDVSSSEFVTFPDVGSHFKIQLPMPLLAGEKYLVQLDGGVVETIGDCPAGNEPVDLPLMIKIPLTKKKFCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H5B4</accession>
    <name>A0A9Q1H5B4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_24221</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H5B4"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="269"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="172"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="239"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="273"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="298"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="352"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="379"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="408"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="460"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8033854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="628" mass="69519" checksum="34239850F568E5D9" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H6D9</accession>
    <name>A0A9Q1H6D9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Sodium-dependent phosphate transport protein 2A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_24542</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8034111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H6D9"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8034111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="145" mass="16121" checksum="AB323295DA772273" modified="2023-09-13" version="1">MALLYFFICSLDLMCSGFYRMGGREAREVLWDKLLDNRICGLLFLILARAGAVLHAGLLLIEQAVLLGASIRASVTAAVVLLVQVCAIPLSFFMRQGRKYQKGGLFQMCVFVLQFCLSDCQGKSHAKLAVLVLFHLNVITFKLVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H757</accession>
    <name>A0A9Q1H757_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Radial spoke head 1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_21930</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Functions as part of axonemal radial spoke complexes that play an important part in the motility of sperm and cilia.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Flagellum axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H757"/>
    <dbReference type="OrthoDB" id="423343at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035082">
        <property type="term" value="P:axoneme assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007286">
        <property type="term" value="P:spermatid development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.110.10:FF:000010">
        <property type="entry name" value="Radial spoke head 1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.110.10">
        <property type="entry name" value="Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43215:SF14">
        <property type="entry name" value="RADIAL SPOKE HEAD 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82185">
        <property type="entry name" value="Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0969">Cilium</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0282">Flagellum</keyword>
    <keyword evidence="7" id="KW-0469">Meiosis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="244"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="271"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="310"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="347"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00056649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00073772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8034905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="369" mass="39586" checksum="307670C1C7BC58F1" modified="2023-09-13" version="1">MAYCIGSRGTQNTMQGVSGKLSGTGDAVILMMQGEYEGERNEKEERHGKGKATLPNGDIYDGMYEHGKRHGQGVYKFKNGARYIGDYKHNKKHGTGTFIYPDGSKYEGNWVDDQRHGYGVYTYPNGDIYEGEWQSHQRHGQGVYTYKDTGSKYVGSWVGGKRDGAGELIHANHRYQGGWIGDMPQGKGKYVFDIGCEQHGQYVPVEQAQGGDAEEEEAQTVTVPKWRAGEIQAISLYNPAEELAKEAEDADAENPENAGEEGAAGDEEGETKEGETKEGEDAAEQPTEGEGAGDTAPGENTTEGDHQPAEGDGQAAPQEEGAAATEGEETHETAATEDPPAEGGGGGEEEAPPPETTEEPQNADETGEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1H8T3</accession>
    <name>A0A9Q1H8T3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_14234</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1H8T3"/>
    <dbReference type="OrthoDB" id="10064933at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8040047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="130" mass="14519" checksum="6586FED912FDB66A" modified="2023-09-13" version="1">MGGIVKSSEKSSRPFKSGHGSVVKSTEKVIIEAKIGKTKCNILTEVVPINLPLLLRKESLKRAETHLNIHQHKATMFGEKIQLEMTSTGHYCVDIIGQRENEEVMYSKSSFKGSDSDNKNRMLFESAKPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HBH0</accession>
    <name>A0A9Q1HBH0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_10100</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HBH0"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8043137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="167" mass="19141" checksum="F90B880E7CE75E4A" modified="2023-09-13" version="1">MNANISRNRAEKRKYERSGRKSGLTVRREIYKEKRNFVTRLIEASRNEDYSTKNSECQGDQKKLFGVEQGLFDTNGEVNLPTHDNQRQPASDGNFFVGKNKLNDDLRRNNVSNPASFTGLSQIRGEKMCTFRLGYEDEIYNLVLQAPATTCDLDPLPSRLLKNDCSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HCA5</accession>
    <name>A0A9Q1HCA5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Netrin receptor UNC5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_11404</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8044052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HCA5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005042">
        <property type="term" value="F:netrin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.220.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037936">
        <property type="entry name" value="UNC5A-D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12582">
        <property type="entry name" value="NETRIN RECEPTOR UNC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12582:SF41">
        <property type="entry name" value="UNC5C-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="268"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="271"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="301"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="322"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8044052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="331" mass="37516" checksum="126D4774462230E4" modified="2023-09-13" version="1">MTIPDTKIVLNIPSNALPENSSGCVIKLRLLPRKVFKDPALTFASNSSVVVELLPNNLILQHPAQLTIPHCLFLEEKKTPTAKIYMSHHQEGCSPCWEEQPGLEYSIGCTHCIVWIKSFCWVTVKFDDKDVLGKHLILYSCGEKLMPDHKYAVTAVGYNPDLDGESKFLKLNKTMIVSQRSPFVFMKEGSHPLTVLLVQYSPEWKLWSPKNRLDIAFEGISSSFEHICKFVFEKTGESAQSLACLYQAFQEKGQQAIDLTLRTESLDSHQDFGSQMRDSSRQTQEVEGNRIQNPELDESQQETSSQGRHTSSYDIRDAAVRPKIHRAQKTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HDM5</accession>
    <name>A0A9Q1HDM5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosome biogenesis protein NOP53</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_08671</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NOP53 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HDM5"/>
    <dbReference type="OrthoDB" id="10057618at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030055">
        <property type="term" value="C:cell-substrate junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17096">
        <property type="entry name" value="FERM_F1_kindlins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037843">
        <property type="entry name" value="Kindlin/fermitin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040790">
        <property type="entry name" value="Kindlin_2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011687">
        <property type="entry name" value="Nop53/GLTSCR2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16160">
        <property type="entry name" value="FERMITIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16160:SF13">
        <property type="entry name" value="FERMITIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00373">
        <property type="entry name" value="FERM_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18124">
        <property type="entry name" value="Kindlin_2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07767">
        <property type="entry name" value="Nop53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Band 4.1" evidence="9">
        <location>
            <begin position="422"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="8">
        <location>
            <begin position="684"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="98"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="238"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="472"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="490"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="503"/>
            <end position="514"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8045627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="935" mass="108058" checksum="421CF944FFD5E791" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HEJ3</accession>
    <name>A0A9Q1HEJ3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_09997</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HEJ3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040314373" description="Secreted protein" evidence="1">
        <location>
            <begin position="25"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8043065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="77" mass="8352" checksum="FB355A7DF92166B8" modified="2023-09-13" version="1">MAAAAWTILGAFTAIVKMATPVLCVQQVKYTMKYVTTYETPFWYTLISVGSSSQLGGGAERSFRRPSWGRGLVGGDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HET8</accession>
    <name>A0A9Q1HET8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_13560</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8042493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HET8"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8042493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="86" mass="10007" checksum="DF543D8F20471ABA" modified="2023-09-13" version="1">MYMHYGKRKNPIVFDGGQRSSGVKKQNFKNSQDRLMSSPEGKKQIFPRIPKSSDVIMSKCKISQEFPRSNMNLKLFFQHVKIALDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HEV5</accession>
    <name>A0A9Q1HEV5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein NEDD1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_05979</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HEV5"/>
    <dbReference type="OrthoDB" id="1602884at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043015">
        <property type="term" value="F:gamma-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007020">
        <property type="term" value="P:microtubule nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052818">
        <property type="entry name" value="NEDD1_Spindle_Assembly"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44414">
        <property type="entry name" value="PROTEIN NEDD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44414:SF1">
        <property type="entry name" value="PROTEIN NEDD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="84"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="180"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="383"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="514"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="390"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="412"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="444"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="534"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="552"/>
            <end position="571"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8047082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="672" mass="73104" checksum="4D4B3978E9325C43" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HF31</accession>
    <name>A0A9Q1HF31_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">EH domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_10760</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8043590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HF31"/>
    <dbReference type="OrthoDB" id="1716625at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045063">
        <property type="entry name" value="Dynamin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027094">
        <property type="entry name" value="Mitofusin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10465:SF4">
        <property type="entry name" value="DYNAMIN N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10465">
        <property type="entry name" value="TRANSMEMBRANE GTPASE FZO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00350">
        <property type="entry name" value="Dynamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="319"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="380"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="Dynamin N-terminal" evidence="7">
        <location>
            <begin position="60"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8043590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="450" mass="51079" checksum="FCE94949C45FFFE6" modified="2023-09-13" version="1">MSGKVRQRGSANPSDAATLSLNERILKDCHNLYTDSEQGLIKIAEKLNLRLLAPRKKITVLLIGNHSAGKSSFINWYIEEHVQRTGVAIETQGFCFVTSGRKRESLTGNATLHLYPHFKELAKLEGVVEYLNTEISTSKQKKFSLVTFVDTPGLVDGDMKYPFDVDKAILWLGDLADLIFVFFDPIGQALCKRTLNIVEKLNEKHSDHMRFYLSKADEAGHESDRQRVLMQIVQELCKRPGLNRTGFDMPTIFVPSMSSGKSRCVNQIEEVCKDVEKTISQMIQNTLNTLETDCEQIASLIDGKLEEDRKAASDNRWAWFKWLTFSLLGFLIPILLATHFLVSNASKDVLAQTVSLPVAEAIKLYLSPMSQMWKTIPKDWHHHVITAIAFISIVFFILAKVTSKIKPRLSRKQKKTLVEQKTYVQSTVKNAKEKLYSEYLKQVVTSQDLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HGR9</accession>
    <name>A0A9Q1HGR9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Histamine H2 receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_07099</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HGR9"/>
    <dbReference type="OrthoDB" id="10042731at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004993">
        <property type="term" value="F:G protein-coupled serotonin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030594">
        <property type="term" value="F:neurotransmitter receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007187">
        <property type="term" value="P:G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24247">
        <property type="entry name" value="5-HYDROXYTRYPTAMINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24247:SF202">
        <property type="entry name" value="5-HYDROXYTRYPTAMINE RECEPTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="53"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="90"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="135"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="176"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="225"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="272"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="10">
        <location>
            <begin position="32"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8044368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="326" mass="36861" checksum="EDA9309CDFE35395" modified="2023-09-13" version="1">MNFTENSLAFKPYITTLYILIYFPVWIAIICGNLVVMLAFVYHRELRRVQNYFLVSLAATDFLTGLVALPLHLVARLVVSQVTCLPSSRFIFFLPVLIFSSASAFHHLCIAIEGLLSLICPLKYSHIVTSCRMKVVIATIWLCNFAFAFSPLYVPHPATDEWVCSYNQHYGDEVKLLYLIAAFGIPAFSTLMFLLYTFMLAIAYQKIKMHRKLSNNVAVIDAKKHFRGVVTMATVVGAFVVCLVPTSFKLFFEIYISATKEVLVTVETLCEFLLFLNSMLNPIIYGCLNQQFRAGFKEVLQSLKCKSALSSFLSLSLQNIINESNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HHK9</accession>
    <name>A0A9Q1HHK9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Reelin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">HOLleu_05700</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">Nanhai2018</strain>
            <tissue evidence="18">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Extracellular matrix serine protease secreted by pioneer neurons that plays a role in layering of neurons in the cerebral cortex and cerebellum by coordinating cell positioning during neurodevelopment. Regulates microtubule function in neurons and neuronal migration. Binding to the extracellular domains of lipoprotein receptors VLDLR and LRP8/APOER2 induces tyrosine phosphorylation of DAB1 and modulation of TAU phosphorylation. Affects migration of sympathetic preganglionic neurons in the spinal cord, where it seems to act as a barrier to neuronal migration. Enzymatic activity is important for the modulation of cell adhesion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Oligomer of disulfide-linked homodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the reelin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="1924787at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070325">
        <property type="term" value="F:lipoprotein particle receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001764">
        <property type="term" value="P:neuron migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000003">
        <property type="entry name" value="Reelin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034968">
        <property type="entry name" value="Reelin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049419">
        <property type="entry name" value="Reelin_subrepeat-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036278">
        <property type="entry name" value="Sialidase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11841">
        <property type="entry name" value="REELIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11841:SF1">
        <property type="entry name" value="REELIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23106">
        <property type="entry name" value="EGF_Teneurin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21471">
        <property type="entry name" value="Reelin_subrepeat-B"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110296">
        <property type="entry name" value="Oligoxyloglucan reducing end-specific cellobiohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50939">
        <property type="entry name" value="Sialidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="11" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="4" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0720">Serine protease</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="970"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="974"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="992"/>
            <end position="1001"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00044961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00046064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAJ8046874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="2287" mass="255849" checksum="7F8617D0883E737F" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HIF8</accession>
    <name>A0A9Q1HIF8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">HHIP-like protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_09274</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HHIP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8046096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HIF8"/>
    <dbReference type="OrthoDB" id="10266706at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018143">
        <property type="entry name" value="Folate_rcpt-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012938">
        <property type="entry name" value="Glc/Sorbosone_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001134">
        <property type="entry name" value="Netrin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018933">
        <property type="entry name" value="Netrin_module_non-TIMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011041">
        <property type="entry name" value="Quinoprot_gluc/sorb_DH_b-prop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008993">
        <property type="entry name" value="TIMP-like_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19328:SF75">
        <property type="entry name" value="ALDOSE SUGAR DEHYDROGENASE YLII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19328">
        <property type="entry name" value="HEDGEHOG-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03024">
        <property type="entry name" value="Folate_rec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07995">
        <property type="entry name" value="GSDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01759">
        <property type="entry name" value="NTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50952">
        <property type="entry name" value="Soluble quinoprotein glucose dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50242">
        <property type="entry name" value="TIMP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50189">
        <property type="entry name" value="NTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040216236" evidence="7">
        <location>
            <begin position="23"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="domain" description="NTR" evidence="8">
        <location>
            <begin position="614"/>
            <end position="738"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8046096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="739" mass="83773" checksum="CBC9DD3468902F72" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HIR6</accession>
    <name>A0A9Q1HIR6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Insulin-like growth factor-binding protein complex acid labile subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_00518</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HIR6"/>
    <dbReference type="OrthoDB" id="1574204at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050328">
        <property type="entry name" value="Dev_Immune_Receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24373:SF378">
        <property type="entry name" value="FI03225P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24373">
        <property type="entry name" value="SLIT RELATED LEUCINE-RICH REPEAT NEURONAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01411">
        <property type="entry name" value="Ephrin_rec_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040182024" evidence="5">
        <location>
            <begin position="22"/>
            <end position="1023"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="573"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="614"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="680"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="734"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="787"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="932"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="962"/>
            <end position="982"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8048269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1023" mass="116207" checksum="D22C62F211100EB6" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HJE7</accession>
    <name>A0A9Q1HJE7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_01769</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HJE7"/>
    <dbReference type="OrthoDB" id="5988894at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051055">
        <property type="entry name" value="PIF1_helicase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47642">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47642:SF8">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8049154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="195" mass="22113" checksum="DBD837C793ECE63B" modified="2023-09-13" version="1">MSKSQRGMSLLLDQACKEARSGNSDIQNQVKSIGNKFLNAVEIGAQEAAYLLLQFPITRSSSNIVFLNTSPPDERTFLLKSKEDLQQMDPDDTDIECGHIIKRYSERPHILKGYCRAVFAFKINLIFPKHIQDPYADCYEDAPFHMNNDSIDEVEFLPGHQTINVTLKSGIQIKSCATPKIIRFVNYNQKTGLEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HJT4</accession>
    <name>A0A9Q1HJT4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Contactin-4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_06684</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HJT4"/>
    <dbReference type="OrthoDB" id="6138780at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000035">
        <property type="entry name" value="Contactin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000064">
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        <property type="entry name" value="DCC isoform 1"/>
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        <property type="entry name" value="palladin isoform X1"/>
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        <property type="match status" value="10"/>
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        <property type="entry name" value="Ig-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR013783">
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        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170:SF6">
        <property type="entry name" value="CONTACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170">
        <property type="entry name" value="PROTEIN SIDEKICK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF13927">
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        <property type="match status" value="3"/>
    </dbReference>
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        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="IG"/>
        <property type="match status" value="6"/>
    </dbReference>
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        <property type="entry name" value="IGc2"/>
        <property type="match status" value="6"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="6"/>
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        <property type="match status" value="4"/>
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        <property type="match status" value="6"/>
    </dbReference>
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    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="17"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="113"/>
            <end position="194"/>
        </location>
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    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="220"/>
            <end position="308"/>
        </location>
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    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="313"/>
            <end position="395"/>
        </location>
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    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="405"/>
            <end position="492"/>
        </location>
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    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="503"/>
            <end position="585"/>
        </location>
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        <location>
            <begin position="589"/>
            <end position="687"/>
        </location>
    </feature>
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        <location>
            <begin position="689"/>
            <end position="787"/>
        </location>
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    <feature type="domain" description="Fibronectin type-III" evidence="9">
        <location>
            <begin position="792"/>
            <end position="888"/>
        </location>
    </feature>
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        <location>
            <begin position="893"/>
            <end position="989"/>
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    </feature>
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        <location>
            <begin position="668"/>
            <end position="701"/>
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    </feature>
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        <location>
            <begin position="974"/>
            <end position="1000"/>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
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        <source>
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        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAJ8047638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1024" mass="111866" checksum="C6C4BA6865E250AC" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HLB3</accession>
    <name>A0A9Q1HLB3_HOLLE</name>
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        <submittedName>
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        </submittedName>
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    </gene>
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        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
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            <taxon>Metazoa</taxon>
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            <taxon>Eleutherozoa</taxon>
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            <taxon>Holothuroidea</taxon>
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            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
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        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="KAJ8049576.1"/>
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            <begin position="50"/>
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        </location>
    </feature>
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        </location>
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    </feature>
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    </feature>
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    </feature>
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        <source>
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        </source>
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        <source>
            <dbReference type="PROSITE" id="PS50164"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="KAJ8049576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="721" mass="83532" checksum="B5181D2CC1DFB89E" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HLG8</accession>
    <name>A0A9Q1HLG8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_02524</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
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        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="component" value="Chromosome 1"/>
    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="76"/>
            <end position="98"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8049678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="100" mass="10773" checksum="7A230CB7C3EE00DF" modified="2023-09-13" version="1">MEDSSKKSESQNAETLPPPYEGEMQEIKLTEPTYPGVVQGLGNDVPSHSGGVPQSRSREAVRDFPGARQASSQAKFWSFAGFFTGLVILVGVILVVVFTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1HLP0</accession>
    <name>A0A9Q1HLP0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Zinc finger CCHC domain-containing protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_02969</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8049978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1HLP0"/>
    <dbReference type="OrthoDB" id="3863715at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002218">
        <property type="term" value="P:activation of innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042509">
        <property type="entry name" value="ZCCHC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22639">
        <property type="entry name" value="GAG-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22639:SF3">
        <property type="entry name" value="ZINC FINGER CCHC DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="160"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="195"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="220"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="226"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="238"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8049978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="247" mass="26770" checksum="6D790E77A911D090" modified="2023-09-13" version="1">MPGGRDSTIVKLEFSSGVSVPPPQAFTELKDNGVDIGEIKMLQALEARNTYDLKLKSDAARVKGVSCLKGVEGLTVTPYDGSVAVTSISAFEGRSAIARVLSQYGTVSEMRWCKYTAGELKGIFNGKRQFRMELVRDIPSFLFIGGSKAHIRYFCQPRTCFRCGEEGHEAKSCPNRRGKCLQLGHGKAECSNEVRCNLCGEEGHVFGSCPTSYSAHVSADVGSAPEPSQTGPQYSTQELEEAALEVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0Y924</accession>
    <name>A0A9Q0Y924_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_44097</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000635">
        <property type="protein sequence ID" value="KAJ8018098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0Y924"/>
    <dbReference type="OrthoDB" id="7691871at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33480">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33480:SF5">
        <property type="entry name" value="SI:DKEY-51D8.9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="105"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="489"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="105"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="130"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="490"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8018098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="526" mass="60633" checksum="EB19663B4207A91D" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0Y965</accession>
    <name>A0A9Q0Y965_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tudor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_43957</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000553">
        <property type="protein sequence ID" value="KAJ8018193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0Y965"/>
    <dbReference type="OrthoDB" id="6105938at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8018193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="81" mass="8934" checksum="8A34B1BE06F52CB1" modified="2023-09-13" version="1">MAYSVGKKVQAVDELGRWEAGKVKAVEQNGTYLVSFDGWGPEFDRNVSSDELREPIEPFVGEQGQVLLRSESGIQAEMSCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0Y9B6</accession>
    <name>A0A9Q0Y9B6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Zinc finger CCHC domain-containing protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_43699</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000420">
        <property type="protein sequence ID" value="KAJ8018352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0Y9B6"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002218">
        <property type="term" value="P:activation of innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042509">
        <property type="entry name" value="ZCCHC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22639">
        <property type="entry name" value="GAG-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22639:SF3">
        <property type="entry name" value="ZINC FINGER CCHC DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCHC-type" evidence="1">
        <location>
            <begin position="133"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="1">
        <location>
            <begin position="152"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8018352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="280" mass="31338" checksum="AABC93683282F1E1" modified="2023-09-13" version="1">MLQPLAARNTYDLTFRSDAARIQGVSCLKGLEGLTVTPYDRSVVVTVLYLNFEVDQGLVARVLSQYGTVSDMRWVTYQAGELKGILNGKRQFRMVLKREIPSFLFIGGSKAHIRYFGQPRTCFKCGERRGTKRCGKCLQVGHDKVECPNKVWCNLCGEEEHVSGSCPTSYSARASAEVGPQYPPHTASRQGNLFLLLVLPPPIPQWNILGLLARVQVPAPHPHWHGQSTMWGTHQRHVPPLHKGCTAPRWDTLPCHAPPAGTCHNVCWDKTWLRVPLVHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YAV3</accession>
    <name>A0A9Q0YAV3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_44214</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000714">
        <property type="protein sequence ID" value="KAJ8018024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YAV3"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8018024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="50" mass="5767" checksum="FBCF62DF85F0C7B5" modified="2023-09-13" version="1">MAIFRAVHVGRCYSSIRLSVAQISQRSVFIVNGRFDHIGMFLCYCWNILS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YDW0</accession>
    <name>A0A9Q0YDW0_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Mutator-like transposase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_40815</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8021053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YDW0"/>
    <dbReference type="OrthoDB" id="6427993at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049012">
        <property type="entry name" value="Mutator_transp_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20700">
        <property type="entry name" value="Mutator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mutator-like transposase" evidence="1">
        <location>
            <begin position="20"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF20700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8021053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="129" mass="14410" checksum="7301BC6284BB2936" modified="2023-09-13" version="1">MSSAPIKKRKLYDRLEEVSGYRIMDMKKFSAALLEIHVCKDGYLQIMEDTDAQKGLCCKFYLVCSKCLTGVPFRSSSLVAHDRSYDVNHRMTTAAAKIGIGRQSLTDLCYMMNMPGAPPVTESNCSTQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YFE8</accession>
    <name>A0A9Q0YFE8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FLYWCH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_38945</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YFE8"/>
    <dbReference type="OrthoDB" id="10541695at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007588">
        <property type="entry name" value="Znf_FLYWCH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04500">
        <property type="entry name" value="FLYWCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FLYWCH-type" evidence="5">
        <location>
            <begin position="41"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="262"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8021673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="289" mass="32947" checksum="00568EED56CEAE33" modified="2023-09-13" version="1">MSIQDRRTFCSQLGKYGGNSQEMVLHPIEELALSSRGRGFIINGYVYVRDKERPGKMYVRCQDRKICQARAVIFPDLNRARVMPTSHTHSPPDISQMRNKKRFHAQRNLSDESTLRMRTQPTTLYTRNSMVDKTRRNTRPMDRAYEARVVPITQSAEYSVKVQIEEQELLESFRRNTNSVPCSSAVDLGVDDLGDEIRCRKPNDTNAEFDKMLEVAGPSSGGKRSVGLYPVSEDYEPYEDGFIPEDEDEEELKPVTVQKVQVEPNTDLNGISPPCPIGKRKHAGSKEFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YHC9</accession>
    <name>A0A9Q0YHC9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_38657</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YHC9"/>
    <dbReference type="OrthoDB" id="6128794at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052787">
        <property type="entry name" value="MAVS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21446">
        <property type="entry name" value="DUF3504 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21446:SF12">
        <property type="entry name" value="POTASSIUM CHANNEL TETRAMERIZATION DOMAIN CONTAINING 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8021450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="231" mass="26690" checksum="20191EA5A3761AA1" modified="2023-09-13" version="1">MSDTCINFDLNFKLESLDDAEPADRFQTLSNQEIEQIARERNEKATTYNTSWGVRTFKAWCKSKGKNPDFEKLSADDLNAFLRVFYAEVRNKQGERFAKQSFVGLRAAIHRHLTGEPFCRQMNILQDREFQAANNVFKGVLKQLKREGHDKSQHKVPISPGDLEKLKTINVSSPRGLQQLAWFYIEYYFGRRGREGLRALPKNCFVFCTDSDGNEYCKMAFNEATKNHPGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YJ35</accession>
    <name>A0A9Q0YJ35_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_38358</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YJ35"/>
    <dbReference type="OrthoDB" id="8060926at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46704:SF9">
        <property type="entry name" value="BHLH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46704">
        <property type="entry name" value="CXC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="152"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8021222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="436" mass="48551" checksum="640AD4AA673E66D0" modified="2023-09-13" version="1">MVGTSSELPTDSSENQNTRNDVKEETSKKIASIGQDILNLSFKGRKCIPKHTSLAMAVRHLTGSAQLIGILNGLGHCVSNFSVLEHDTALAELQLERGEQYLPPIIKRNVHSILVWDNNDFREETLSGKGSTHNTNGIIIQRNIINREAATESTTFHTSKPKTRKRSIDLPPPPPPASILNYFGGKRQGPQASGKEIKTDEASYLSAETNPTKFAMAYFLSKLPEYETPVPPGWTGFNTKLQKASAVPEKSNIGYLPVIDASPTNLSTVNTILQRSLAIADQLGLETIVVVMDQAIYSKAQQIRWKDAMYMKRIILWLGEFHTSMSFLACVGKRFRDAGLQDVMIESELVAQGSVSTVMNGHHYNRSVRAHKIVAEVLQRLRLQAFLDNLTNAEKEAAIGLCVQLKQTFPGEEFVRLVECEEFQDLLSQYEHFIEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YJH6</accession>
    <name>A0A9Q0YJH6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">5'-nucleotidase</fullName>
            <ecNumber evidence="3">3.1.3.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_36245</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a ribonucleoside 5'-phosphate + H2O = a ribonucleoside + phosphate</text>
            <dbReference type="Rhea" id="RHEA:12484"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:18254"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
            <dbReference type="EC" id="3.1.3.5"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the 5'-nucleotidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.5" evidence="3"/>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YJH6"/>
    <dbReference type="OrthoDB" id="7722975at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008253">
        <property type="term" value="F:5'-nucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006196">
        <property type="term" value="P:AMP catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000020">
        <property type="entry name" value="NT5E isoform 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.780.10">
        <property type="entry name" value="5'-Nucleotidase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008334">
        <property type="entry name" value="5'-Nucleotdase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036907">
        <property type="entry name" value="5'-Nucleotdase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006146">
        <property type="entry name" value="5'-Nucleotdase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006179">
        <property type="entry name" value="5_nucleotidase/apyrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11575:SF24">
        <property type="entry name" value="5'-NUCLEOTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11575">
        <property type="entry name" value="5'-NUCLEOTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02872">
        <property type="entry name" value="5_nucleotid_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01607">
        <property type="entry name" value="APYRASEFAMLY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55816">
        <property type="entry name" value="5'-nucleotidase (syn. UDP-sugar hydrolase), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00785">
        <property type="entry name" value="5_NUCLEOTIDASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040544179" description="5'-nucleotidase" evidence="8">
        <location>
            <begin position="24"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="9">
        <location>
            <begin position="28"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="5'-Nucleotidase C-terminal" evidence="10">
        <location>
            <begin position="350"/>
            <end position="509"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8023725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="545" mass="59937" checksum="668CCE00987146B3" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YJS9</accession>
    <name>A0A9Q0YJS9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Meiosis 1 arrest protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_36477</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YJS9"/>
    <dbReference type="OrthoDB" id="6433824at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051308">
        <property type="term" value="P:male meiosis chromosome separation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007127">
        <property type="term" value="P:meiosis I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033587">
        <property type="entry name" value="M1AP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28642">
        <property type="entry name" value="MEIOSIS 1 ARREST PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28642:SF1">
        <property type="entry name" value="MEIOSIS 1 ARREST PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8023905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="116" mass="12521" checksum="5FEA4D388C20548C" modified="2023-09-13" version="1">MCHLLQEKELAILAKKDTPPPPSYSKVGGGVYLKPSSSRPITTPSSAGHFLILPSHGMSMLVKSVAVKELMLPSDAHEKGEPPLSDTLETVQKVLEKVLLHTNLLILSKEEASLGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YKX6</accession>
    <name>A0A9Q0YKX6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Immunoglobulin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_39508</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8022107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YKX6"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="342"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="Immunoglobulin" evidence="2">
        <location>
            <begin position="35"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Immunoglobulin" evidence="2">
        <location>
            <begin position="250"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8022107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="403" mass="46311" checksum="93CB6B73B6F73DFF" modified="2023-09-13" version="1">MAKRTRETRTYFALIIFIPFHCFVAETNITFERDPPNYIVFEGNPVFLVCEVEINDPVVINHDGIAIVEDSTLRGSSSKYTFLRHTRNNEYILKINRVERSDSGIYVCRGGILIVKQLNLEVYYHPSSAPLCTSSHDASKYFFEDMLNKEFEFNCTVEDGNPQSEVLIFQEFGKRERMALNAIVVNKNEGINPRRMVTFSSKLNVSFNNSSFICDVRQEIPPDLEVNDYRRVCFYGPMTFISNFQTFVSPRNLTIKEGDSKILFCYSNVQSADKKWMTNVTEGVEMEMIERGKETSLTLSVVEGFEYEIVTLVCESSFENRYSSATVQIHITREKSKNCSAVLVPLIVILFILMMVVVIFAFREICSKFVTKSSFGREDHDLRIEETVDDTAGKLMKNETEVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YNZ0</accession>
    <name>A0A9Q0YNZ0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Pancreas transcription factor 1 subunit alpha</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_35079</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8025001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YNZ0"/>
    <dbReference type="OrthoDB" id="10048995at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032502">
        <property type="term" value="P:developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11417">
        <property type="entry name" value="bHLH_TS_PTF1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000035">
        <property type="entry name" value="Pancreas-specific transcription factor 1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050283">
        <property type="entry name" value="E-box_TF_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23349:SF112">
        <property type="entry name" value="48 RELATED 1, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23349">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, TWIST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="6">
        <location>
            <begin position="65"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="27"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="174"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="238"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="198"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="244"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8025001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="262" mass="29622" checksum="366E4AB7DB56DBCD" modified="2023-09-13" version="1">METIPFDTHNGYMYQNSHHMYHPYHNGGMLHPLPQPHISNRRHTDQDGSKTRRRRKPKCIIQQVQQRQAANLRERKRMQSINDAFEGLREHIPTLPYEKRLSKVDTLRLAIGYINFLSEIIENEDSENDLLDKVPTENQKKVIICHRGSPTPTETEFGLQPLAGHSLSWSNERKKSTYGSSHTLTAKVWTPEDPRGLNSPTGSTEMNTSTGSVAESISFDENDSLLSPLSEDNSYLTLEKSDDLLSPMDSSPGSFCDSPSAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YQ10</accession>
    <name>A0A9Q0YQ10_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_31256</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YQ10"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007157">
        <property type="term" value="P:heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051427">
        <property type="entry name" value="Nectin/Nectin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23277:SF108">
        <property type="entry name" value="FASCICLIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23277">
        <property type="entry name" value="NECTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="236"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="9">
        <location>
            <begin position="54"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="160"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="406"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="503"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="210"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="408"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="445"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="519"/>
            <end position="528"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8026440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="528" mass="58365" checksum="0E73C08EB0378F93" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YQ51</accession>
    <name>A0A9Q0YQ51_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_36978</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8024282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YQ51"/>
    <dbReference type="OrthoDB" id="10068969at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331:SF6">
        <property type="entry name" value="DOUBLECORTIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8024282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="142" mass="16155" checksum="831DBA2EDC315A1A" modified="2023-09-13" version="1">MLKKELVTKGLVKFDDNPVTHRVWKATFKGVVEGLGLSAREEADLLVKWLGPKPSKLMMRLREVHPRDPESGLKTLWSRLEREYGAPELIECLLLSQQENFSKIGQRKPKKLQELTDLLPEIAAAKKDVYFPGLVFLDTSKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YQE2</accession>
    <name>A0A9Q0YQE2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_31403</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YQE2"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8026544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="54" mass="6101" checksum="9AA6E79BE134A5A7" modified="2023-09-13" version="1">MIQNIMSVSSLHVVRHPSLASFKSYGRNTGVKVSIAIPQLRYNVKMVHCMKEIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YQF8</accession>
    <name>A0A9Q0YQF8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Estradiol 17-beta-dehydrogenase 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_35816</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YQF8"/>
    <dbReference type="OrthoDB" id="9876299at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008202">
        <property type="term" value="P:steroid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43313:SF50">
        <property type="entry name" value="GH26015P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43313">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE/REDUCTASE FAMILY 9C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="36"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="331"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="331"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8023386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="361" mass="40326" checksum="5BB9778E95B9239F" modified="2023-09-13" version="1">MSLKLILYSAITIFFNGAVLRRYLKDELLLAGPYTLLVILPTITQPVCQLIFGGFCGITLFAIASYQIYKRLPQQFVNYDDKVVFISGCDSGFGHETAKRLDAKGVHVIAGCLLKGESGEQDLVKSCSDRLVTIQLDVTKEDSVQNAVKEVKEKLQGRDLWALVNNAGILYIAEAEIMSLKYVRSMMDVNFMGAVRLTKAFLPMLRQSEGRVVSLCSIGTLLAKENVNNKLEAVYQEMEPSLKEDYGPRYKDEWFRIFTAGYEASQDLSPVINTIEHALFAKSPLTMYNPELSGEVFRWINICLPVSFTKHLLGALVMSGNFISKDFQKVTEKDQNADEVKRGDAGNNKHTDQSGEGDSEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YS32</accession>
    <name>A0A9Q0YS32_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_31724</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YS32"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="73"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="58"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="109"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="165"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="175"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="189"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8026786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="298" mass="34000" checksum="6FC95D330ADF730B" modified="2023-09-13" version="1">MVKRGERSGQDEPKIPEGTQGEKADDQKPDTATGHIWMEQVPQPNERRRSSRPRRLVYKMEWRDEDVEASREYRCPICKKKYSSGPELEHLQRTHPKGKRPLEDTSKNASRKKRDVKKHSSKDTMHQQDQAGPSRQEQEGKKTTAVPGGGRMTREESHQRSFGYLPLLDDLLGTPRPPTPRSPTPPKTQLDEANTKEIPTEVQGTAGPDPRLFKPIQRGSAATEAQEVMEKAREKARSTTSSHTSCRWAIQRIQESEKKYIITTETAVGEDGRFYRMTKRSSTRSEEDSLCKYIVICS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q0YSW4</accession>
    <name>A0A9Q0YSW4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Guided entry of tail-anchored proteins factor 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Tail-anchored protein insertion receptor WRB</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Tryptophan-rich basic protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_32169</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WRB/GET1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q0YSW4"/>
    <dbReference type="OrthoDB" id="69461at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043529">
        <property type="term" value="C:GET complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043495">
        <property type="term" value="F:protein-membrane adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071816">
        <property type="term" value="P:tail-anchored membrane protein insertion into ER membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.660">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028945">
        <property type="entry name" value="Get1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029012">
        <property type="entry name" value="Helix_hairpin_bin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42650:SF1">
        <property type="entry name" value="GUIDED ENTRY OF TAIL-ANCHORED PROTEINS FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42650">
        <property type="entry name" value="TAIL-ANCHORED PROTEIN INSERTION RECEPTOR WRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04420">
        <property type="entry name" value="CHD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="101"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="137"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="41"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00033006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8027120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="187" mass="21579" checksum="23A9D889808D7973" modified="2023-09-13" version="1">MKTRHNKMAASMALAVFAILVPLFALKLSIPSITKTVLSLLHKQGEQKLHLQAELRKLKEEQDGIDIKDEFAKHAKLQRKINKVTDELKSIRNSKFKRHLIIQWGVTIFLHFVHKLCYLMVIWSLREKPVIQMKYEWTWPIGSILAFPCGQPGAIGITWWVVASSLVIRESHRLLSAFRSNSSKKEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1B919</accession>
    <name>A0A9Q1B919_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_43400</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000295">
        <property type="protein sequence ID" value="KAJ8018546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1B919"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8018546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="104" mass="12031" checksum="13BD961C548B4B95" modified="2023-09-13" version="1">MSASAFCVLHGFGRFVYIFFIFCHNLYCYLRQLCSRNLSPYFSITCMLKKYTVPLHRIKRGAKRLIAICSSTCTVEQFSQLSCAFVLHMQIVKKKKTLFGVKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1B9Q6</accession>
    <name>A0A9Q1B9Q6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_43432</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000305">
        <property type="protein sequence ID" value="KAJ8018530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1B9Q6"/>
    <dbReference type="OrthoDB" id="10066064at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33480">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33480:SF1">
        <property type="entry name" value="TYR RECOMBINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8018530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="158" mass="18278" checksum="23E7A9EB471F66B2" modified="2023-09-13" version="1">MDVPESERQYIYKHMGHSEETNQHIYQAPLAVKELTVVGKCLQTIDEASLVVTDGCVTEDETGEGTPEETGDEGLESGVFKHGRKCCKWTNEESSIVKNYFQKYFQGIGDKRLPGRKEVLAFLEENKLNKDWKTIHTKVMNEVRKIEKIAEQRRKQWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1B9S8</accession>
    <name>A0A9Q1B9S8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Carbamoyl-phosphate synthase [ammonia], mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_43199</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000230">
        <property type="protein sequence ID" value="KAJ8018680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1B9S8"/>
    <dbReference type="OrthoDB" id="434at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004088">
        <property type="term" value="F:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CPAse_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005483">
        <property type="entry name" value="CPSase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036914">
        <property type="entry name" value="MGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11405:SF53">
        <property type="entry name" value="CARBAMOYL-PHOSPHATE SYNTHASE [AMMONIA], MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11405">
        <property type="entry name" value="CARBAMOYLTRANSFERASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00098">
        <property type="entry name" value="CPSASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52335">
        <property type="entry name" value="Methylglyoxal synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040166874" evidence="4">
        <location>
            <begin position="19"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="Carbamoyl phosphate synthase ATP-binding" evidence="5">
        <location>
            <begin position="29"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8018680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="285" mass="32121" checksum="1F072BE75E91D406" modified="2023-09-13" version="1">MIFKILQVLSTLCHRLYAMPSQNVENAGVHSGDATLILPPQTISSEAIEKVKYATQKVAERFQISGPFNMQFLAKKNDVMVIECNIQASRSFPFVSKTINCDMINIATKAMIGEHFDQSLLPLLNNSFTPEDYVGIKAMFILCFFLFCLDLNLYKYHLFATEKTAKYLQDHNIPAFTVVWPLAVMKEEEREYPPATRYYITVVDELNNLLKNSFQITTSACKRNRALACNFLHLISSPFSNLPGTNKGFEVVNLFAEALDEVGNLDASALFHFTKPSGSNFSKEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1B9Y1</accession>
    <name>A0A9Q1B9Y1_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein uS9</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">40S ribosomal protein S16</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_44984</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010001553">
        <property type="protein sequence ID" value="KAJ8017538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1B9Y1"/>
    <dbReference type="OrthoDB" id="426865at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000754">
        <property type="entry name" value="Ribosomal_uS9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21569:SF16">
        <property type="entry name" value="RIBOSOMAL PROTEIN S16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21569">
        <property type="entry name" value="RIBOSOMAL PROTEIN S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00380">
        <property type="entry name" value="Ribosomal_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00043019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8017538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="87" mass="9809" checksum="5159B9F9EB4E7147" modified="2023-09-13" version="1">MNEKFPYGETLLKYYVPFFFSKMSQMTEQSQQGSGARLPPQSVQVFGRKKTATAVAHCKRGNGLVKVNGKPLHLIEPRGLQYKVTFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BAS3</accession>
    <name>A0A9Q1BAS3_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_43920</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000530">
        <property type="protein sequence ID" value="KAJ8018214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BAS3"/>
    <dbReference type="OrthoDB" id="416119at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31635">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31635:SF196">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="2">
        <location>
            <begin position="101"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="2">
        <location>
            <begin position="118"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase" evidence="3">
        <location>
            <begin position="434"/>
            <end position="725"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8018214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="902" mass="102905" checksum="DF754F79B2D3D8E2" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BAS8</accession>
    <name>A0A9Q1BAS8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_43888</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000516">
        <property type="protein sequence ID" value="KAJ8018229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BAS8"/>
    <dbReference type="OrthoDB" id="5985848at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008737">
        <property type="entry name" value="DUF1758"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040676">
        <property type="entry name" value="DUF5641"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331:SF1">
        <property type="entry name" value="GAG-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47331">
        <property type="entry name" value="PHD-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05585">
        <property type="entry name" value="DUF1758"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18701">
        <property type="entry name" value="DUF5641"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="8">
        <location>
            <begin position="320"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8018229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="657" mass="73564" checksum="DAE979E9016CF664" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BB38</accession>
    <name>A0A9Q1BB38_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_42029</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8020150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BB38"/>
    <dbReference type="OrthoDB" id="6274754at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061343">
        <property type="term" value="P:cell adhesion involved in heart morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007508">
        <property type="term" value="P:larval heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33395:SF22">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33395">
        <property type="entry name" value="TRANSCRIPTASE, PUTATIVE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8020150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="151" mass="16824" checksum="91E27AEA900F6B38" modified="2023-09-13" version="1">MKVMFANVRSLFNKFIEFLAHISIDSPDIQVVGVTEPWLHSDISNSEIQVPGYKIFRQDRADTVCGRGGGVLLYVKDSINCVDVTNKFSSGFSNYLWCEISFVNESCTPLVVGIIYHSPNSCADNNQSLLESFRKVSSKRAVVFGDFSPSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BBR7</accession>
    <name>A0A9Q1BBR7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_41298</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000023">
        <property type="protein sequence ID" value="KAJ8019638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BBR7"/>
    <dbReference type="OrthoDB" id="10059291at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025398">
        <property type="entry name" value="DUF4371"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057456">
        <property type="entry name" value="Znf_C17orf113"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46880:SF5">
        <property type="entry name" value="DUF4371 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46880">
        <property type="entry name" value="RAS-ASSOCIATING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14291">
        <property type="entry name" value="DUF4371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25431">
        <property type="entry name" value="zf-C17orf113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C17orf113 probable zinc finger" evidence="3">
        <location>
            <begin position="249"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4371" evidence="2">
        <location>
            <begin position="359"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="166"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="195"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8019638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="889" mass="100636" checksum="FAED78F0619B85E5" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2025-10-08" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BBZ2</accession>
    <name>A0A9Q1BBZ2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Reverse transcriptase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_42797</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000136">
        <property type="protein sequence ID" value="KAJ8018932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09274">
        <property type="entry name" value="RNase_HI_RT_Ty3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01647">
        <property type="entry name" value="RT_LTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22249">
        <property type="entry name" value="UDM1_RNF168_RNF169-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000115">
        <property type="entry name" value="Polyprotein of retroviral origin, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.370:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 17.6-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000032">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 297-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.70:FF:000001">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon gypsy-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054465">
        <property type="entry name" value="Integrase_p58-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041373">
        <property type="entry name" value="RT_RNaseH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22938">
        <property type="entry name" value="Integrase_p58_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17917">
        <property type="entry name" value="RT_RNaseH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="9">
        <location>
            <begin position="892"/>
            <end position="1050"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase" evidence="8">
        <location>
            <begin position="1306"/>
            <end position="1483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="94"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="359"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="625"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="725"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="364"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="376"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="400"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="625"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="649"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="669"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="694"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="741"/>
            <end position="765"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAJ8018932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1698" mass="195040" checksum="6C060D8C774F4683" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BC20</accession>
    <name>A0A9Q1BC20_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_40944</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8021155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BC20"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040455675" evidence="2">
        <location>
            <begin position="23"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="134"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8021155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="217" mass="23479" checksum="F56B01034CAEFE43" modified="2023-09-13" version="1">MMKTGIAVTFCVFVINCAYVSSQDSPEGLWCPRDVPPGINASAEPDCSYYSNITCPPNQGCCGVSDRVNGYGYFHLCVELNEEPADCINDPQLDFVDGMSMKHGEHKSSCGSSCNCHNGIVNCGEKICLRAGDILSIIAVLGSILIAALFCICLCCYCSYKIWQPRRSDQTNASQTEDNPPSYKTAVRMPISISESISRDGISPPTYDEAVNIAEGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BDP9</accession>
    <name>A0A9Q1BDP9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SCAN box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_38614</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BDP9"/>
    <dbReference type="OrthoDB" id="10063366at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888:SF13">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46888">
        <property type="entry name" value="ZINC KNUCKLE DOMAINCONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCAN box" evidence="3">
        <location>
            <begin position="227"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="321"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="78"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="321"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="336"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="360"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8021424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="556" mass="62746" checksum="50D88664AD23E33D" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BDQ2</accession>
    <name>A0A9Q1BDQ2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Acetylcholine receptor subunit alpha-type acr-16</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_37917</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the ligand-gated ion channel (TC 1.A.9) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000020">
        <property type="protein sequence ID" value="KAJ8022895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BDQ2"/>
    <dbReference type="OrthoDB" id="5975154at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005230">
        <property type="term" value="F:extracellular ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18997">
        <property type="entry name" value="LGIC_ECD_nAChR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19051">
        <property type="entry name" value="LGIC_TM_cation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000030">
        <property type="entry name" value="AcetylCholine Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="207"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="237"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="270"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="305"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="459"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="7">
        <location>
            <begin position="2"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="8">
        <location>
            <begin position="212"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="423"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8022895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="483" mass="56064" checksum="BD044265520FF4BB" modified="2023-09-13" version="1">MDYLMRRYKYKVRPVMNETTVVQVKHEIVPRRILYVSEIRQEMKIHYWVRMEWQDEFLVWDPDDFNGTTTIFIPSEALWHPDIVLYQSNGDPISVRSSSVIPLIVSFTGRVLLLTPKIYTTHCKMNLTFYPFDTQTCYLKFGSWAYDGLKLDIVSRVEEAESSSYLHNGEWDLKKVTVERHEFYYSCCPEPYPDVEYTLYLTRRPMFYVMNLITPFIAITLLSSFAFIIPPDSGEKVSLAVTTLLSLVVFTQLVMSTVPAASDGTHPVIGVYFVVSVVLVSVVVVCSAISLHAFHKKDSGMPVPYLVRLVVLDLMGGVCRKLRLLHSSNNIYRHNVTDISTSTTNRDLERLQWSRETEKVLNLDREIDGSLRNRSEIYTISNERDCPTFIPRSLGREGTNQRVPSSPRSATISSIHRMEQLLLKMELCQREEKKRSNEEEERKKESYEWMQVSRVLDRFLLIVFTIIHIIVPAIILMKCSRIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BDS7</accession>
    <name>A0A9Q1BDS7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Hyalin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">HOLleu_40716</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">Nanhai2018</strain>
            <tissue evidence="10">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000022">
        <property type="protein sequence ID" value="KAJ8020972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="OrthoDB" id="10066451at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.960">
        <property type="entry name" value="SEA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003410">
        <property type="entry name" value="HYR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000082">
        <property type="entry name" value="SEA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036364">
        <property type="entry name" value="SEA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24273">
        <property type="entry name" value="FI04643P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24273:SF32">
        <property type="entry name" value="HYALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02494">
        <property type="entry name" value="HYR"/>
        <property type="match status" value="33"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01390">
        <property type="entry name" value="SEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
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</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
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                <person name="Chen T."/>
            </authorList>
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        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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</entry>
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    <name>A0A9Q1BFT0_HOLLE</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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            <authorList>
                <person name="Chen T."/>
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            <tissue evidence="2">Muscle</tissue>
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    </reference>
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        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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        <location>
            <begin position="6"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8026121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="86" mass="9935" checksum="0AADC9B565F79CA9" modified="2023-09-13" version="1">MSCVCVYVCVCVCVCVCACVCVCVCVFVTLLVIAISQDRIDRHFSYLVYRFVMLRGRTLLFLEEVEGHRRSPEVKNRKPCNRVILR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BG22</accession>
    <name>A0A9Q1BG22_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Secretion-regulating guanine nucleotide exchange factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_35652</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000019">
        <property type="protein sequence ID" value="KAJ8023282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BG22"/>
    <dbReference type="OrthoDB" id="10256179at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058923">
        <property type="entry name" value="RCC1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051625">
        <property type="entry name" value="Signaling_Regulatory_Domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22872">
        <property type="entry name" value="BTK-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25390">
        <property type="entry name" value="WD40_RLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00633">
        <property type="entry name" value="RCCNDNSATION"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00626">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="5"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="RCC1-like" evidence="3">
        <location>
            <begin position="8"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="56"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="105"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="156"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="210"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="270"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="330"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8023282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="380" mass="40536" checksum="009CB06D934452D9" modified="2023-09-13" version="1">MFRKQNRIVCGANSYGQLSIGNTEDSVVPVRSSNVEGEIKQVTGGGGHTAIVTEEGKLYLCGNNNKGQLGLDSAVNLHLQCLHLPSAISKVACGWEHTLAITASGDLIAWGSNSHGQAGQEVVSSSLPIKLKGFNGQPVCDIAAGLRHSLAVTIDGLVWSWGCGKKGQLGWMEAKKGLTYSHQPQQVPGCQKAHLVAAGAYHSIMLTVDGELFCWGDNKRGQMAIEPCKDKGTFVLDRPRQLDLRPLESGHRIIAVASGWTHVLILTDGGDIYSWGRNDYGQLGRSSVCHNPDGSPRGDICWQIVKIVQLNNIKQIACGSEHNLAVTEDGVICSWGWNEHGMCGDGTTQDIPEPKTVPALTGVRGHLIGSGAGHSVVVCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BGM4</accession>
    <name>A0A9Q1BGM4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Tyrosine-protein kinase transmembrane receptor Ror</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_34367</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BGM4"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004714">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00192">
        <property type="entry name" value="PTKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000554">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050122">
        <property type="entry name" value="RTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416:SF620">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR TORSO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="10 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="38"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="106"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00051243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8024454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="449" mass="51039" checksum="FA58BB07072DD4B2" modified="2023-09-13" version="1">MIILKPRPSNHALKNNTNTTPTPTPTTPSAPSETEQPFLVYIFIAGITVLLVFILCLLLIRLGILRKRTSKEERPVFSIRVSNHSSEVPVLPEFRDKEIINIYDVELSEEAIGSGQFGEVFKATLNLGEGNLNKQTVAVKRSKDNLRAALKEEFLDEIRLMIEIGNHPNIMPLLACRTVAEPYYLITEYMKYGSLKDYLHKSSIPEIVSQDPNYDLTDLRKLQICHQISKGMEYLATTRFFHGDLAARNVLINEEMVAKISDFGLSNDIYAKGYVRLPEEQKRPVKWYSPEANHFGKCSSEGDVWSFGVVLYEIYSGGQEPYPGMLPREVAVRVKAGYRMDRPENCPKQIYIIMRDCWQYEPSDRPSFELIRQKFDEMLSRGDDPYVHSIGDHERVVDNDSSSAGNVERVSVESSIYDASLNITYSRLVPNVEEDPKVSTENNKGQDGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BHA8</accession>
    <name>A0A9Q1BHA8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_34687</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000018">
        <property type="protein sequence ID" value="KAJ8024707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BHA8"/>
    <dbReference type="OrthoDB" id="5977701at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35450">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35450:SF2">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8024707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="243" mass="27657" checksum="05338771CB36ED3F" modified="2023-09-13" version="1">MDDPDVDTWQTNQWLRSAGLKAETGGLIVAAQDQSLATRAYLHRKVKDGTNPLCRLCGQYKETIDHIISGCPVLAPTEYTHRHNKVATYIHWLICRHYGIETSTDKWYDHQPLTVSENQDATILWDMPVHTDREIKANRPDIVVKDFKEKKCLLIDVAVPSDKNISIKTTEKLSKYKDLEIEICRMWQMKTETVPVVVGALGMCKKGMNAYLNKLPGQIDMNLVQKTALLGTAHILRKMLSIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BIG1</accession>
    <name>A0A9Q1BIG1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">JmjC domain-containing protein 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_31692</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8026762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BIG1"/>
    <dbReference type="OrthoDB" id="415358at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041667">
        <property type="entry name" value="Cupin_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12461">
        <property type="entry name" value="HYPOXIA-INDUCIBLE FACTOR 1 ALPHA INHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12461:SF18">
        <property type="entry name" value="JMJC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13621">
        <property type="entry name" value="Cupin_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="JmjC" evidence="4">
        <location>
            <begin position="184"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="408"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="505"/>
            <end position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8026762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="524" mass="60199" checksum="57C314D2C3062D1D" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BIU6</accession>
    <name>A0A9Q1BIU6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">2-oxoisovalerate dehydrogenase subunit beta, mitochondrial</fullName>
            <ecNumber evidence="3">1.2.4.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Branched-chain alpha-keto acid dehydrogenase E1 component beta chain</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_32548</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Together with BCKDHA forms the heterotetrameric E1 subunit of the mitochondrial branched-chain alpha-ketoacid dehydrogenase (BCKD) complex. The BCKD complex catalyzes the multi-step oxidative decarboxylation of alpha-ketoacids derived from the branched-chain amino-acids valine, leucine and isoleucine producing CO2 and acyl-CoA which is subsequently utilized to produce energy. The E1 subunit catalyzes the first step with the decarboxylation of the alpha-ketoacid forming an enzyme-product intermediate. A reductive acylation mediated by the lipoylamide cofactor of E2 extracts the acyl group from the E1 active site for the next step of the reaction.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[protein] + 3-methyl-2-oxobutanoate + H(+) = N(6)-[(R)-S(8)-2-methylpropanoyldihydrolipoyl]-L-lysyl-[protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:13457"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:10497"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83142"/>
            <dbReference type="EC" id="1.2.4.4"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:13458"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="9">Heterotetramer of 2 alpha/BCKDHA and 2 beta chains/BCKDHB that forms the branched-chain alpha-keto acid decarboxylase (E1) component of the BCKD complex. The branched-chain alpha-ketoacid dehydrogenase is a large complex composed of three major building blocks E1, E2 and E3. It is organized around E2, a 24-meric cubic core composed of DBT, to which are associated 6 to 12 copies of E1, and approximately 6 copies of the dehydrogenase E3, a DLD dimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.4.4" evidence="3"/>
    <dbReference type="EMBL" id="JAIZAY010000016">
        <property type="protein sequence ID" value="KAJ8027412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BIU6"/>
    <dbReference type="OrthoDB" id="878at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003863">
        <property type="term" value="F:branched-chain 2-oxo acid dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009083">
        <property type="term" value="P:branched-chain amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007584">
        <property type="term" value="P:response to nutrient"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07036">
        <property type="entry name" value="TPP_PYR_E1-PDHc-beta_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.920:FF:000004">
        <property type="entry name" value="2-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000001">
        <property type="entry name" value="Pyruvate dehydrogenase E1 beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033248">
        <property type="entry name" value="Transketolase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42980:SF1">
        <property type="entry name" value="2-OXOISOVALERATE DEHYDROGENASE SUBUNIT BETA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42980">
        <property type="entry name" value="2-OXOISOVALERATE DEHYDROGENASE SUBUNIT BETA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02780">
        <property type="entry name" value="Transketolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="TK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Transketolase-like pyrimidine-binding" evidence="12">
        <location>
            <begin position="54"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00051764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00057409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00063295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00071568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00082400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8027412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="375" mass="41209" checksum="326D566691F3CAB6" modified="2023-09-13" version="1">MASITGLTCKTFAPISLFLRSLRKSLPKNLVRNASAFTFHPQDPDKSLGETAKMNLFQSLTNAMDIALATDQTAVIFGEDVAFGGVFRCTVGLADKYGKDRVFNTPLCEQGIVGFGIGMASVGATAIAEIQFADYIYPAYDQIVNEAAKFRYRSGNQFNVGGLTVRSPWGAVGHGALYHSQSPEAAFASCPGLKVVIPRGPTQAKGLLLSCIRDRNPCIYFEPKVLYRAAVEEVPVQDYMIPLSQAEVILEGNDITLVGWGTQVHVLWEVAELAKEKLGVSCELIDLQTILPWDEETVVKSVQKTGRLLIAHEAPLTGGFAAEIASTIQEQCFLHLECPIQRVCGWDTPFPHIFEPFYLPDKWRCLEAIRKAVNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BKJ9</accession>
    <name>A0A9Q1BKJ9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Tumor necrosis factor receptor superfamily member EDAR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_30565</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000015">
        <property type="protein sequence ID" value="KAJ8028357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BKJ9"/>
    <dbReference type="OrthoDB" id="10017617at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043123">
        <property type="term" value="P:positive regulation of canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046330">
        <property type="term" value="P:positive regulation of JNK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.10">
        <property type="entry name" value="Tumor Necrosis Factor Receptor, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001875">
        <property type="entry name" value="DED_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001368">
        <property type="entry name" value="TNFR/NGFR_Cys_rich_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047526">
        <property type="entry name" value="TNR19/27/EDAR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12120">
        <property type="entry name" value="TNFR-CYS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12120:SF10">
        <property type="entry name" value="TNFR-CYS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00208">
        <property type="entry name" value="TNFR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50168">
        <property type="entry name" value="DED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00652">
        <property type="entry name" value="TNFR_NGFR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50050">
        <property type="entry name" value="TNFR_NGFR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 14" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="192"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="TNFR-Cys" evidence="12">
        <location>
            <begin position="52"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="repeat" description="TNFR-Cys" evidence="9">
        <location>
            <begin position="52"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="DED" evidence="13">
        <location>
            <begin position="329"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="270"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="279"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="294"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ8028357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="434" mass="48092" checksum="77B911F22D5D7B77" modified="2023-09-13" version="1">MKVRKRSIDSCGEYEFLNTSTNACQDCVRCPPGLELDKECGWGEGQQAVCRECPAGSYQDGSDFYCSPCSYCKNREIIQTCAASHNTKCGGCKPWYIFNERIKMCVPCKPADNCDPPQGLATTARTGPLKSLTPSPGNITATVAIATSDKTTAVLLLNRVNTGTRGPAEMLPGSSHNVTSLKTNSSKIPSSIVSVIIFVVLSLTLLILGLCIFQRRAEPGSCSQILLGTCRERRREPQPRKELTSNSTNKEVQTNLLAVEYNVRDENTLTHITRKGQTSESSGSSSLISEDTLIDSQTPSSQSSAHHSREQIAGIATYSITFTNEERQAYSAEIHKISDELEARQFETLVSYVWSAVENFTECDKEAAKSFLDLQRILDQLREYCMTQPCILVIGLRVVRRFDLAERLFEVTKCTHHPNICKTEIPFRRQGTTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BMT6</accession>
    <name>A0A9Q1BMT6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Bifunctional peptidase and (3S)-lysyl hydroxylase JMJD7</fullName>
            <ecNumber evidence="11">1.14.11.63</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_28926</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.63" evidence="11"/>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BMT6"/>
    <dbReference type="OrthoDB" id="415358at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106155">
        <property type="term" value="F:peptidyl-lysine 3-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000059">
        <property type="entry name" value="jmjC domain-containing protein 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041667">
        <property type="entry name" value="Cupin_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12461:SF99">
        <property type="entry name" value="BIFUNCTIONAL PEPTIDASE AND (3S)-LYSYL HYDROXYLASE JMJD7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12461">
        <property type="entry name" value="HYPOXIA-INDUCIBLE FACTOR 1 ALPHA INHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13621">
        <property type="entry name" value="Cupin_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="JmjC" evidence="13">
        <location>
            <begin position="130"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00066577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00071397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ8029515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="320" mass="37450" checksum="2D73D992E3FC0531" modified="2023-09-13" version="1">MDGDERSVSELFDYFEALSREARELYLGQTVPILEKPPSPLRFHREWVSPNKPVIIKNAVTSWPALEKWDLNYLSQILQNKKVSVAVTPNGLADAVYEDHFVLPEERKIKFEEFVHLLEHQNNKDGIFYIQQQDSNLIREFPELLCDVDPEISWASEALNKKPDAVNLWIGSQESVSSMHKDHFENLYVVITGAKHFILHPPTDQPFIPYEEFPVASYKEISEGHFEIEPSNPASVVKWIPVDPKKPDLLKWPLYSKVQPFHCTVHPGEMLYLPSMWYHHVSQDKNTIAVNYWYDMEFDIKFNYFQFVEKLTTSLKNVKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BNH1</accession>
    <name>A0A9Q1BNH1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_29446</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000014">
        <property type="protein sequence ID" value="KAJ8029917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BNH1"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8029917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="77" mass="8879" checksum="C903207B81824FDC" modified="2023-09-13" version="1">MESSREFNFNAAFLECSRRNCLRDATLLNCYHPALPSLVIIRSWNQFLRYSTLQVYYCTIVNVHALPALGPDQANIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BPT6</accession>
    <name>A0A9Q1BPT6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">LINE-1 retrotransposable element ORF2 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_26761</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8030364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BPT6"/>
    <dbReference type="OrthoDB" id="6241411at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19446">
        <property type="entry name" value="REVERSE TRANSCRIPTASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8030364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="299" mass="34093" checksum="65D316E0E67F8957" modified="2023-09-13" version="1">MWLANAIATAFFYSRHALHTTFRLPTLRFVCPLANILDAPPIRNWHLIDYVIIRKDEQNNVRVTKARCGVPQAPQLWKSKKADEIQTFADSKNVRCFYEALNTVYGPRSSRSSPVLNADDTKLLTDRKQILERWAEHFNSVLNRPLSINNNAIDRLPQVKTNYTLGDLPIEHQVEKAIHQMPCGKAPGSDSIPAEVFKVGGQALLKRLTQLYQLMWKEEQLPQQFKDTTVVHIYKQKGNPQSCDNHRGISLLSFAGKTFARILLNRLLPHLKQDLLPESQCGSRAGRGTVDMIFVTRQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BR59</accession>
    <name>A0A9Q1BR59_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_27969</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000013">
        <property type="protein sequence ID" value="KAJ8031286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BR59"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8031286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="265" mass="30328" checksum="634F0B6C245FB5F8" modified="2023-09-13" version="1">MTVSQRTVPDRYSSSQKVQILPSSLLLAPPTTVFSFFLSRHKPSLLLPVVSWSWVIFSDCLFIKCSLLLDLSHFFGIPGYPKNSRKCNLQPQACKVTHQIKISLPRPGTVHPILQTKFYPSQRPKQSPNHLADRHAVQINPPFVTYDINTEAENITVCFGFLEGLFILLESPPNPLRAFASKGMTPTPHCSAVLCIDSFWWPSLTQMPFIELWRYPCRVSSIFQQAYLRLDYHWHSTVNPSIYPCMAFPERAHYEHDITILARSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BWH4</accession>
    <name>A0A9Q1BWH4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transposable element Tc1 transposase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_20841</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BWH4"/>
    <dbReference type="OrthoDB" id="4843387at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038717">
        <property type="entry name" value="Tc1-like_DDE_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13358">
        <property type="entry name" value="DDE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tc1-like transposase DDE" evidence="1">
        <location>
            <begin position="125"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8034127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="250" mass="29269" checksum="6D7B1D9A8F0B5319" modified="2023-09-13" version="1">MLECGLTESDISVRSVQRFFNQKGYRYLQARRKGLLKRTDLRKRLMFAKDIKKQRTRNFWTKDVMFYFNGTGFVHKRNPMQQALSPKGRIWRKPSEALQRVRSYGKGVVLCKSYKKLDGPKLATFVRRYFPRTMKACGTKRQKIFLQDGDPSRNSIHAKAAFADLGLRVLKIPPRSPDLNPIENFFHIASKKLCKDALEKRIQDETFRQFKKRAIRSLKAIPKATIDKIIFSMDKRVDMLISNGGNRLKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BWJ8</accession>
    <name>A0A9Q1BWJ8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GIY-YIG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_24231</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000011">
        <property type="protein sequence ID" value="KAJ8033861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BWJ8"/>
    <dbReference type="OrthoDB" id="3598281at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="CDD" id="cd10442">
        <property type="entry name" value="GIY-YIG_PLEs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1440.10">
        <property type="entry name" value="GIY-YIG endonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000305">
        <property type="entry name" value="GIY-YIG_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035901">
        <property type="entry name" value="GIY-YIG_endonuc_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058912">
        <property type="entry name" value="HTH_animal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21301">
        <property type="entry name" value="REVERSE TRANSCRIPTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21301:SF10">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01541">
        <property type="entry name" value="GIY-YIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26215">
        <property type="entry name" value="HTH_animal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82771">
        <property type="entry name" value="GIY-YIG endonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helix-turn-helix" evidence="2">
        <location>
            <begin position="23"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="GIY-YIG" evidence="1">
        <location>
            <begin position="217"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8033861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="326" mass="37480" checksum="E908A226F35D7018" modified="2023-09-13" version="1">MVILNNGIISTDVFSKPTDGHLYLLPSSSHPKHQVKSIPYSIALRLKRICSTPELLAKRVIEFKHYLIARGYDPQLVEEQFEKANKLPRSALLNPAKKSNSNRKYPLVLDFNPALPNANSIIRKHMHILHSSPRMTNLFPENSFFTAYRKCKSLKDLLRKSKSPYRTRTGDTVGCFKCERRRCDLCTNFLLQSSNFSSCATKKTYPIKSYVSCTSACIIYVATCFKCNLQYVGSTSTEFKVRFRNHKSDLLNNRGRCELAVHYNSSPHQLSDIKFIIIEQTYSKSNNDTALTKREGFWLAQLNTLFPLGLNKRKEFNSSKRISYLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BWM7</accession>
    <name>A0A9Q1BWM7_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_20994</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BWM7"/>
    <dbReference type="OrthoDB" id="8035941at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF11">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8034241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="183" mass="21242" checksum="EC2C8B4617B38D47" modified="2023-09-13" version="1">MNKVFLKLIRTLVVEGTDWRKELKNFLLINRSSPHITTGLSPAEMLFNRQIRTKLPQVGNMSVSQKQLKEAMRHDRAQKQSYKNYADRHRRTQVRDIKPGYKVLVRCEKKNKLSPTFFPTPFKVAKKMGKCLELMRPDGIKFRENVSHTKLYKCGEGEIGDANLYDLEEDDTCDNGEGENIHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BX50</accession>
    <name>A0A9Q1BX50_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_21994</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8034953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BX50"/>
    <dbReference type="OrthoDB" id="5973140at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="CDD" id="cd10442">
        <property type="entry name" value="GIY-YIG_PLEs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1440.10">
        <property type="entry name" value="GIY-YIG endonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000305">
        <property type="entry name" value="GIY-YIG_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035901">
        <property type="entry name" value="GIY-YIG_endonuc_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058912">
        <property type="entry name" value="HTH_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21301">
        <property type="entry name" value="REVERSE TRANSCRIPTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21301:SF10">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01541">
        <property type="entry name" value="GIY-YIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26215">
        <property type="entry name" value="HTH_animal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="19"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8034953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="695" mass="79914" checksum="666F152385B6F230" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BYQ0</accession>
    <name>A0A9Q1BYQ0_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">HOLleu_22619</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">Nanhai2018</strain>
            <tissue evidence="1">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000010">
        <property type="protein sequence ID" value="KAJ8035396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BYQ0"/>
    <dbReference type="OrthoDB" id="10044445at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8035396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="185" mass="20811" checksum="1AD4EA10074E5EEA" modified="2023-09-13" version="1">MAVILSLRNIVEMVHSPVFTNTTVGHLQTYLKELKIIFKKAFPDSRFKPKHHFSEHYPDQIRLLGPLVHVSTIRFEAKHNFFKRIIRGISNFKSILATLARRYQLMEAYHLASPHFLGQSVVVKNVTGIHATLLSEDIQKVLPDMLPSSIILVSDTVVIEGTKYSNGMFGLVASATLGLAQFAEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1BZM2</accession>
    <name>A0A9Q1BZM2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">AN1-type zinc finger protein 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_19294</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000009">
        <property type="protein sequence ID" value="KAJ8035573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1BZM2"/>
    <dbReference type="OrthoDB" id="428577at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1110.10:FF:000001">
        <property type="entry name" value="Zinc finger AN1-type containing 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1110.10">
        <property type="entry name" value="AN1-like Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035896">
        <property type="entry name" value="AN1-like_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050652">
        <property type="entry name" value="AN1_A20_ZnFinger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002653">
        <property type="entry name" value="Znf_A20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000058">
        <property type="entry name" value="Znf_AN1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10634:SF149">
        <property type="entry name" value="AN1-TYPE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10634">
        <property type="entry name" value="AN1-TYPE ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01754">
        <property type="entry name" value="zf-A20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01428">
        <property type="entry name" value="zf-AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00259">
        <property type="entry name" value="ZnF_A20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00154">
        <property type="entry name" value="ZnF_AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118310">
        <property type="entry name" value="AN1-like Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51036">
        <property type="entry name" value="ZF_A20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51039">
        <property type="entry name" value="ZF_AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="A20-type" evidence="6">
        <location>
            <begin position="10"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="AN1-type" evidence="7">
        <location>
            <begin position="143"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="46"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="46"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="126"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8035573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="208" mass="21832" checksum="1B8DC612C5D2CFEB" modified="2023-09-13" version="1">MDSGNGSKEIPSGNLCRNGCGFYGSAATEGMCSKCFKDYQRKKQNSPALAASSATTPTGISSGGLSSPIASSIPDLPVSTASTTTPSLTPTTTLNQSTDLETDGAAAAAASTYKDLNSEKEPSPSPTTSSSSSDGGSEGEKGKKKRNRCHTCRKKVGLTGFVCRCGGLFCSIHRYSDKHECSFDYRTHGKEEIRKNNPVVVGEKIQKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C2C8</accession>
    <name>A0A9Q1C2C8_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">HOLleu_18115</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">Nanhai2018</strain>
            <tissue evidence="6">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C2C8"/>
    <dbReference type="OrthoDB" id="10056483at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15489">
        <property type="entry name" value="PHD_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46364">
        <property type="entry name" value="OS08G0421900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="5">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="5">
        <location>
            <begin position="52"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAJ8037322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="174" mass="19177" checksum="F3E9E17917761DEE" modified="2023-09-13" version="1">MCAKLVKKNDNAVQCDGCDHWVHIRCADIDREGYKLLQLSKPEDKWYCSKCSSPCGICLKGVRGDDPAIECDSCKKWIHNRCSLADDSHYDHIQATNCIWMCPNCDNGNLSNTWGLTAPQTENSFHALNPDSGNAHSIPSIDGKSSARRPSGKTALKKEVSIVSININGLRGKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C2Q4</accession>
    <name>A0A9Q1C2Q4_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">E3 ubiquitin-protein ligase PDZRN3-B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">HOLleu_18085</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">Nanhai2018</strain>
            <tissue evidence="7">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8037297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C2Q4"/>
    <dbReference type="OrthoDB" id="1630758at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10131">
        <property type="entry name" value="TNF RECEPTOR ASSOCIATED FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10131:SF94">
        <property type="entry name" value="TNF RECEPTOR-ASSOCIATED FACTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00504">
        <property type="entry name" value="Ubox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="38"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="149"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="149"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAJ8037297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="214" mass="24509" checksum="7818FDEC53C20C6C" modified="2023-09-13" version="1">MPRRCKRSRKSSAVADRLEEEEDRYFLNPESVSSHLYCSVCQEVFKDPQRAPCGHSFCRVCIYKWLQQSKTCPEDRRPLRTQDLHSDFIVANIIGDQLVACPYKRKGCEHICKLELLKSHSKSCDFNPCNLPGFLADDDNDNLLAQEQPHSSSVMGLHNLPSSSMTEPDKLPSPVKPSLKMRLFRNGGDTRQTLCAMFERQTENKENEDIICLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C3B5</accession>
    <name>A0A9Q1C3B5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BRICHOS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_15898</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C3B5"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="74"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8038463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="279" mass="31732" checksum="27186361AFAA1E8E" modified="2023-09-13" version="1">MAAEKTSLHQDVPVVCTVPSCPEKKFIRTERHHLNLEDMQVGDCSLPTSRCSDVSIFRQVYIERASATDSRVRVLGMVLVTVFIVAGFLGVVYLMSSPRTDKSYVPSSESSDNIYLNGIKEVTVEFNYQETSITEVVKVEDNRQFVTITDIKDGFIVTLDYKRGIAVVKNTSSSDCFFTRLENFPEYQTEDMDNLRPILVEEVDEMPETNHTEETVNLRRSEVIPWEYVQVTSDPYIAGQCTDATSYWLQVNPTHTVQRRDGNCGCVIIIIVCRCRAQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C4J6</accession>
    <name>A0A9Q1C4J6_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_18984</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000008">
        <property type="protein sequence ID" value="KAJ8038019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C4J6"/>
    <dbReference type="OrthoDB" id="6091153at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19963">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19963:SF30">
        <property type="entry name" value="ENDONUCLEASE_EXONUCLEASE_PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="837"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="268"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="452"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="576"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="816"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="845"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="8"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="845"/>
            <end position="860"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8038019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="868" mass="97236" checksum="F8FB46305F6E6CB1" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C4X5</accession>
    <name>A0A9Q1C4X5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Tripartite motif-containing protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_15883</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000007">
        <property type="protein sequence ID" value="KAJ8038452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C4X5"/>
    <dbReference type="OrthoDB" id="6105938at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05819">
        <property type="entry name" value="NHL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001258">
        <property type="entry name" value="NHL_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050952">
        <property type="entry name" value="TRIM-NHL_E3_ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24104">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE NHLRC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24104:SF25">
        <property type="entry name" value="PROTEIN LIN-41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01436">
        <property type="entry name" value="NHL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101898">
        <property type="entry name" value="NHL repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51125">
        <property type="entry name" value="NHL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="7">
        <location>
            <begin position="18"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="repeat" description="NHL" evidence="6">
        <location>
            <begin position="302"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8038452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="504" mass="56229" checksum="7E3AD8B77457E936" modified="2023-09-13" version="1">MEDKRASSIENLQMNVWCSICHEPWTRPKTLPCDHIFCEECLEAVLRRTDGNSLPCPVCRTSYEVPSQGLHEAWTTSRTLENLADGFRSMEFSSPGSQLSTNSASPEHSYITNPPPKKIPIGQLIRIVVQLCNQDGQLIPAQSGRYDITGYVDLKPRPFGFERKELKATVSTQGNPYIEFQVHSCGFHEFTVQLENRSIKGSPLGISVVPRDILGCEVKGPLLGPRDVIPYEGNFLVSDGIAKAVFLVDQTGQKLQSLNIPKKMAEMFCPMAIATFQQKVFVADMKNRCIHVYENLNDFPKQFGEEQLQKPTGVTVSPDNGNIFIVDNERKLVVVFNQDYKYLKTINYRASSTEDQLFNPQMAVINKNGDKLYIADHGDRGKENFIKIINVFEDKLERKISVRVGKIQARPCGMDIDSNGNMYVTCTFPEFGKRHGEGKPVKLNFTGGINMYNSLGQFLCHFGAQLKRPNGLTVLPPASNSASIVYVADSPGNSQNGSLKGFIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C8I2</accession>
    <name>A0A9Q1C8I2_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_11991</name>
        <name evidence="1" type="ORF">HOLleu_38470</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000021">
        <property type="protein sequence ID" value="KAJ8021308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C8I2"/>
    <dbReference type="OrthoDB" id="2113965at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAJ8021308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8041238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="63" mass="7395" checksum="94096B844FFDEAD8" modified="2023-09-13" version="1">MPHIERKNPIVFGGGQRSPGVTGGQTLKTLLTQYLEVGSLDEFHTLYVDALWYEEEPYHFWWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C8R3</accession>
    <name>A0A9Q1C8R3_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Phosphatidylinositol phosphatase PTPRQ</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">HOLleu_14722</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">Nanhai2018</strain>
            <tissue evidence="4">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C8R3"/>
    <dbReference type="OrthoDB" id="6381660at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050713">
        <property type="entry name" value="RTP_Phos/Ushers"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46957">
        <property type="entry name" value="CYTOKINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46957:SF3">
        <property type="entry name" value="CYTOKINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040347868" evidence="2">
        <location>
            <begin position="20"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="741"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="3">
        <location>
            <begin position="234"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="3">
        <location>
            <begin position="331"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="3">
        <location>
            <begin position="439"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="3">
        <location>
            <begin position="542"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="3">
        <location>
            <begin position="641"/>
            <end position="735"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAJ8040432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="849" mass="94532" checksum="604AFC96F4B022C1" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C8W6</accession>
    <name>A0A9Q1C8W6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Telomerase protein component 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">HOLleu_14381</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">Nanhai2018</strain>
            <tissue evidence="5">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C8W6"/>
    <dbReference type="OrthoDB" id="2325716at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080008">
        <property type="term" value="C:Cul4-RING E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051191">
        <property type="entry name" value="DCAF12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025139">
        <property type="entry name" value="DUF4062"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19860">
        <property type="entry name" value="DDB1- AND CUL4-ASSOCIATED FACTOR 12-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19860:SF14">
        <property type="entry name" value="DUF4062 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13271">
        <property type="entry name" value="DUF4062"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="DUF4062" evidence="4">
        <location>
            <begin position="52"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="NACHT" evidence="3">
        <location>
            <begin position="314"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="589"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1026"/>
            <end position="1053"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="595"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1034"/>
            <end position="1043"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1044"/>
            <end position="1053"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAJ8040161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="1053" mass="120607" checksum="6FCE64159E21CE2F" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C9G4</accession>
    <name>A0A9Q1C9G4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Dynein axonemal intermediate chain 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">WD repeat-containing protein 78</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_14797</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Flagellum axoneme</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Dynein axonemal particle</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C9G4"/>
    <dbReference type="OrthoDB" id="10259804at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005858">
        <property type="term" value="C:axonemal dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120293">
        <property type="term" value="C:dynein axonemal particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045504">
        <property type="term" value="F:dynein heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045503">
        <property type="term" value="F:dynein light chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003341">
        <property type="term" value="P:cilium movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000373">
        <property type="entry name" value="WD repeat domain 78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000379">
        <property type="entry name" value="WD repeat domain 78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050687">
        <property type="entry name" value="Dynein_IC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442:SF12">
        <property type="entry name" value="DYNEIN AXONEMAL INTERMEDIATE CHAIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442">
        <property type="entry name" value="DYNEIN INTERMEDIATE CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0969">Cilium</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0282">Flagellum</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 12" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="12">
        <location>
            <begin position="669"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="297"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="21"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="333"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ8040489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="821" mass="90202" checksum="6416839EE61BF588" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1C9P4</accession>
    <name>A0A9Q1C9P4_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Elongation of very long chain fatty acids protein</fullName>
            <ecNumber evidence="10">2.3.1.199</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Very-long-chain 3-oxoacyl-CoA synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">HOLleu_15378</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">Nanhai2018</strain>
            <tissue evidence="11">Muscle</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a very-long-chain acyl-CoA + malonyl-CoA + H(+) = a very-long-chain 3-oxoacyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:32727"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:90725"/>
            <dbReference type="ChEBI" id="CHEBI:90736"/>
            <dbReference type="EC" id="2.3.1.199"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the ELO family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.199" evidence="10"/>
    <dbReference type="EMBL" id="JAIZAY010000006">
        <property type="protein sequence ID" value="KAJ8040927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1C9P4"/>
    <dbReference type="OrthoDB" id="434092at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009922">
        <property type="term" value="F:fatty acid elongase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034625">
        <property type="term" value="P:fatty acid elongation, monounsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034626">
        <property type="term" value="P:fatty acid elongation, polyunsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019367">
        <property type="term" value="P:fatty acid elongation, saturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030457">
        <property type="entry name" value="ELO_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002076">
        <property type="entry name" value="ELO_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157:SF126">
        <property type="entry name" value="ELONGATION OF VERY LONG CHAIN FATTY ACIDS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157">
        <property type="entry name" value="FATTY ACID ACYL TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01151">
        <property type="entry name" value="ELO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01188">
        <property type="entry name" value="ELO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="5 10" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2 10" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7 10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="32"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="116"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="146"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="170"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="204"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="234"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAJ8040927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="266" mass="31886" checksum="F126626CE895AD29" modified="2023-09-13" version="1">MATFAQRVTDFYDYMLEKGDPRVEDWFLMKNPLPIIAIEIFYLVGVVWLGPRIMANRQPFNLKGLMIVYNGFLVLLSMYMFYEFRISSWLANYSYRCQPVDYSNDPLALRMANVCWWYFFSKVIELLDTIIFVLRKKNNQISFLHVFHHSTMIINWWLGVKYIAGGQSWFLAMCNSFIHILMYSYYALAAIGPQMQKFLWWKRYMTQMQLIQFVLVVCHTGYNIYIDCNFPQGFNYAVFAYGIFLIILFSNFYIRSYFAKKEKQKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CAQ5</accession>
    <name>A0A9Q1CAQ5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Copine-8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HOLleu_12798</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Nanhai2018</strain>
            <tissue evidence="15">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the copine family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000005">
        <property type="protein sequence ID" value="KAJ8041876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CAQ5"/>
    <dbReference type="OrthoDB" id="5855668at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071277">
        <property type="term" value="P:cellular response to calcium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd04048">
        <property type="entry name" value="C2A_Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04047">
        <property type="entry name" value="C2B_Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01459">
        <property type="entry name" value="vWA_copine_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000042">
        <property type="entry name" value="Copine 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037768">
        <property type="entry name" value="C2B_Copine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045052">
        <property type="entry name" value="Copine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010734">
        <property type="entry name" value="Copine_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10857:SF106">
        <property type="entry name" value="C2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10857">
        <property type="entry name" value="COPINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07002">
        <property type="entry name" value="Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="C2" evidence="13">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="13">
        <location>
            <begin position="72"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="14">
        <location>
            <begin position="253"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="493"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="502"/>
            <end position="517"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAJ8041876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="517" mass="58824" checksum="D736AB2F940AE49E" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CCE6</accession>
    <name>A0A9Q1CCE6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">ARF GTPase-activating protein GIT2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">HOLleu_09610</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">Nanhai2018</strain>
            <tissue evidence="12">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8042761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CCE6"/>
    <dbReference type="OrthoDB" id="5588096at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032012">
        <property type="term" value="P:regulation of ARF protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008277">
        <property type="term" value="P:regulation of G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036465">
        <property type="term" value="P:synaptic vesicle recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08833">
        <property type="entry name" value="ArfGap_GIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047161">
        <property type="entry name" value="GIT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032352">
        <property type="entry name" value="GIT1/2_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022018">
        <property type="entry name" value="GIT1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013724">
        <property type="entry name" value="GIT_SHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46097:SF3">
        <property type="entry name" value="ARF GTPASE-ACTIVATING PROTEIN GIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46097">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR KINASE INTERACTING ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12205">
        <property type="entry name" value="GIT1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16559">
        <property type="entry name" value="GIT_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08518">
        <property type="entry name" value="GIT_SHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00405">
        <property type="entry name" value="REVINTRACTNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00555">
        <property type="entry name" value="GIT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50115">
        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 8" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Arf-GAP" evidence="11">
        <location>
            <begin position="1"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="160"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="351"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="492"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="386"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="417"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="492"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="513"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="567"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="620"/>
            <end position="637"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAJ8042761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="759" mass="84506" checksum="5A61E1EBCEF8D78D" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CCQ1</accession>
    <name>A0A9Q1CCQ1_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Matrix metalloproteinase-16</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_09813</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="10">Can bind about 5 Ca(2+) ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="10">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M10A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8042926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CCQ1"/>
    <dbReference type="OrthoDB" id="406838at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030574">
        <property type="term" value="P:collagen catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04278">
        <property type="entry name" value="ZnMc_MMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.110.10.10">
        <property type="entry name" value="Hemopexin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036375">
        <property type="entry name" value="Hemopexin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018487">
        <property type="entry name" value="Hemopexin-like_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033739">
        <property type="entry name" value="M10A_MMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001818">
        <property type="entry name" value="Pept_M10_metallopeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021190">
        <property type="entry name" value="Pept_M10A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036365">
        <property type="entry name" value="PGBD-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201">
        <property type="entry name" value="MATRIX METALLOPROTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201:SF291">
        <property type="entry name" value="MATRIX METALLOPROTEINASE 1, ISOFORM C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00413">
        <property type="entry name" value="Peptidase_M10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001191">
        <property type="entry name" value="Peptidase_M10A_matrix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00138">
        <property type="entry name" value="MATRIXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00120">
        <property type="entry name" value="HX"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50923">
        <property type="entry name" value="Hemopexin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47090">
        <property type="entry name" value="PGBD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <keyword evidence="6 9" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040406993" evidence="11">
        <location>
            <begin position="21"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase metallopeptidase" evidence="12">
        <location>
            <begin position="124"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="243"/>
        </location>
    </feature>
    <feature type="binding site" description="in inhibited form" evidence="10">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="246"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="319"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="321"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>5</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="364"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="366"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>5</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="412"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>5</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001191-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001191-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR621190-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8042926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="542" mass="61753" checksum="69165827891534F5" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CF81</accession>
    <name>A0A9Q1CF81_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_11441</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8044077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CF81"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8044077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="98" mass="10698" checksum="AC561011EDA8564C" modified="2023-09-13" version="1">MYTKCVNFPPMTKISLEIPKIPGKEPPCFPTFSTQENRMKLALVDGGRVGGGAYKAMQSRDVAMVGCAGRPHPALDISARHYRQTHVSPPTTNLPTET</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CFP9</accession>
    <name>A0A9Q1CFP9_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dynein regulatory complex protein 10</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">IQ domain-containing protein D</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">HOLleu_11501</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">Nanhai2018</strain>
            <tissue evidence="14">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the nexin-dynein regulatory complex (N-DRC), a key regulator of ciliary/flagellar motility which maintains the alignment and integrity of the distal axoneme and regulates microtubule sliding in motile axonemes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the nexin-dynein regulatory complex (N-DRC). Interacts with CFAP52.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Flagellum axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DRC10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000004">
        <property type="protein sequence ID" value="KAJ8044121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CFP9"/>
    <dbReference type="OrthoDB" id="536093at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="CDD" id="cd23767">
        <property type="entry name" value="IQCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042815">
        <property type="entry name" value="DRC10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31598:SF1">
        <property type="entry name" value="DYNEIN REGULATORY COMPLEX PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31598">
        <property type="entry name" value="IQ DOMAIN-CONTAINING PROTEIN D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0969">Cilium</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0282">Flagellum</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="15"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="141"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="330"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="394"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="15"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="141"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="161"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00046836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAJ8044121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="483" mass="56764" checksum="5D1F8FCC3AC57E53" modified="2023-09-13" version="1">MSFADMAVMETFRLLDKVRSEGPKERAERPNPQPPQRYKPVHKPRPPKQPQIKTDPLSILDPGRKKLTTVESQRVLAVLDESIKKIEVVSLLQYAVQNIDKYRNVMGDEMLTALQEHGNLQNNLRDTFLKLKEYREAKQKAAEEEARRREEEEENTGSEEGSPRESIKSQEGEDEPDEKEEYLVKTIKVQEYQVTFSCKNILRMLALTPSLVNSLRSEVKERDENASQMISHLKDLRGFILERLLTTPLEEKDKTEYLLEINVREKKNSEVIKKLSSELEAEIAEKDAEISKRNDEIRRLKGDRHQLEKFSEEHIRRTRLEADKSQQADLKNSEGKQATLQAEVQKLRNKLATMTAEHRDSELTLRKRKYKIETEVENWIQKYDQDMGERQEEYEDLDTIYTEEKAQLNELEERFKTLEAEYTTIVEERRIAQEKREQEQRELAAMIKAATIVQAFWRSYKCRKMLKAKNKKKGKKGKKGKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CGE5</accession>
    <name>A0A9Q1CGE5_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Lectin 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_07726</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8044852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CGE5"/>
    <dbReference type="OrthoDB" id="6133475at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050111">
        <property type="entry name" value="C-type_lectin/snaclec_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22803">
        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C-type lectin" evidence="1">
        <location>
            <begin position="39"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8044852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="167" mass="18866" checksum="E0F0BB5359044B16" modified="2023-09-13" version="1">MKPSHLRTATVRYVGQIDEEGAEHMLLLGLHMDDAEHVTWEEGEKECNTFQEGGVTGHLVSIHSAAEQSFVYELFKSLTAAPTTTRNPWFPQLNIGLRVEQGKSLSWSDGSPVDYTNWYSGEPNGWNAYGQISDGTGKNGLWYDNDNAYLRPFMCKIPRPSYELTYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CGU8</accession>
    <name>A0A9Q1CGU8_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Solute carrier family 35 member F6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">HOLleu_08013</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">Nanhai2018</strain>
            <tissue evidence="9">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CRT-like transporter family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000003">
        <property type="protein sequence ID" value="KAJ8045082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CGU8"/>
    <dbReference type="OrthoDB" id="29773at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013936">
        <property type="entry name" value="CRT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13146">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13146:SF8">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER F6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08627">
        <property type="entry name" value="CRT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="106"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="163"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="196"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="236"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="279"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="310"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="351"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="383"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="392"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAJ8045082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="416" mass="47029" checksum="D2880A72D7291936" modified="2023-09-13" version="1">MALSVYQFALVAGMLITGSINTLSKKAQNDCRVKGIPYNRSNSTSGEEHEFDHPWFQTIIMFLGESFCLIGLIVSRYRERSRHRRNLYLSNIQGDGERIELKQPRIFQFVFLLPTLCDLIGTTLAGIGLLYVTASVWQMLRGSLIIFTGIFSKVFLKRKLRWYHWTGMIVTVMGLLLVGSASIFEDQTSNGVKETILGFVFILAGQASNALQMVVEETFLKNKNYPPLQVVGMEGTFGFIIMAFIVLPILYFIPDESQFSKRYEDTIDALYQIANSPKLLAFCLLYLFSITFYNYCGLAVTKSLTAVHRTLIDACRTIVVWVVDLIIFYAFGGSFGEAFNTTYGLLQIDGFMFLLIGTALYNNLFDLSFLPCLREQSSQRNAENSQAVTQAEEAKHGTDDERAPLLQQEVEIVPQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CJS9</accession>
    <name>A0A9Q1CJS9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">POC1 centriolar protein-like A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_05558</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the WD repeat POC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8046772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CJS9"/>
    <dbReference type="OrthoDB" id="10264588at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048871">
        <property type="term" value="P:multicellular organismal-level homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000235">
        <property type="entry name" value="POC1 centriolar protein homolog B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050505">
        <property type="entry name" value="WDR55/POC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44019:SF8">
        <property type="entry name" value="POC1 CENTRIOLAR PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44019">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="14"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="56"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="98"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="140"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="182"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="224"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="266"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="315"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="434"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00037984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8046772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="480" mass="53069" checksum="C9AD9360279E5E2F" modified="2023-09-13" version="1">MASVLEDPTLERNYKGHRDTVTSVDFNPNMKQLASGGMDSCLMVWNFKAQMRAFRFVGHKDAVLSVNFSPSGHLVASASRDKTVRLWIPSVKGESTVFKAHTATVRSVQFSCDGQNLLTCSDDKTLKVWSVHRQKFQFSLSQHMNWVRCAKWSPDGRLIVSGSDDKTIKLWDRNSKECIHTFFEHGGFVHSVAFHPSGTCIAAAGTDSTVKVWDIRMNKLLQHYQAHAGAVNSISFHPSGNYLITASNDATLKILDLLEGRLFYTLHGHQGPATAVSFSRNGEHFASGGADEQVLVWKTNFDKVDYNEVLQSHRARTQVQPPPHIHDIHPRSPGYKQAVRPLGSEVNAHIEPRDMGNKTPTVTNLGPALFSQHRVRFSGRSGPSAGAGLHDLDITSSDMEARSMHMEGTPGGLAGSSTAPLEVKEMPAGLAKTLENMVGQLDVLTQTVNILEQRLTMTENKLREVLDNQQKITLQVRPTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CM13</accession>
    <name>A0A9Q1CM13_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Synapsin-3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">HOLleu_06088</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">Nanhai2018</strain>
            <tissue evidence="8">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Synapse</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the synapsin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000002">
        <property type="protein sequence ID" value="KAJ8047165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CM13"/>
    <dbReference type="OrthoDB" id="10249572at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007269">
        <property type="term" value="P:neurotransmitter secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000008">
        <property type="entry name" value="Synapsin III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001359">
        <property type="entry name" value="Synapsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020898">
        <property type="entry name" value="Synapsin_ATP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019736">
        <property type="entry name" value="Synapsin_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020897">
        <property type="entry name" value="Synapsin_pre-ATP-grasp_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10841">
        <property type="entry name" value="SYNAPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10841:SF17">
        <property type="entry name" value="SYNAPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02078">
        <property type="entry name" value="Synapsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02750">
        <property type="entry name" value="Synapsin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10581">
        <property type="entry name" value="Synapsin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01368">
        <property type="entry name" value="SYNAPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0770">Synapse</keyword>
    <feature type="domain" description="Synapsin pre-ATP-grasp" evidence="6">
        <location>
            <begin position="71"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="Synapsin ATP-binding" evidence="7">
        <location>
            <begin position="175"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="27"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="28"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="42"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAJ8047165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="254" mass="29509" checksum="1C61DDCDC0BAC079" modified="2023-09-13" version="1">MNFLRRRFSDPNSLGFLPDGYFQNLGAEDSKEKEKEKEKVIASTAPNSTATTPSPRRDLGFLSNVMGKQEKGKYKTLLVIDDHHTDWSKYFRGKKVHGEFDIKVEQCEFSEINLSASCDTGVVLDMKSSRQGLQVMRTFRPDFVLVRQHPQSMEVQEDWRNTIIGFQYGNVPSINSWQALYNFMDKPWVFAQLSVLQEKYGKEKFPLVDQTFFPNHKEMVSSLLLVFCKTMDVALQSIIQWILKKKKKMTTQRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CNP6</accession>
    <name>A0A9Q1CNP6_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Tyrosine-protein kinase TXK</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">HOLleu_00939</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">Nanhai2018</strain>
            <tissue evidence="13">Muscle</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8048573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CNP6"/>
    <dbReference type="OrthoDB" id="5782147at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013162">
        <property type="entry name" value="CD80_C2-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050198">
        <property type="entry name" value="Non-receptor_tyrosine_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24418">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08205">
        <property type="entry name" value="C2-set_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="329"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="216"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="433"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="387"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAJ8048573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="652" mass="73544" checksum="DD761D2A8AE84520" modified="2023-09-13" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CSH5</accession>
    <name>A0A9Q1CSH5_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF4806 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_03377</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CSH5"/>
    <dbReference type="OrthoDB" id="10227797at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032071">
        <property type="entry name" value="DUF4806"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34153:SF2">
        <property type="entry name" value="SI:CH211-262H13.3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34153">
        <property type="entry name" value="SI:CH211-262H13.3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16064">
        <property type="entry name" value="DUF4806"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4806" evidence="2">
        <location>
            <begin position="173"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="38"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8050245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="282" mass="30401" checksum="A8CD085198A81604" modified="2023-09-13" version="1">MPFESDSDEDDSPSEPVPKKKTAPSLSVPPPCPPMPTLPSADRIPSSSPSGSRETPSTSESSLSEPRAGTSRSVSPLPTGSRTPTTSVQIDAAATPGPSSLTPSRGEKATSLQRAVSLRAAELGVTATDARMLELLEAILLQVTKNSKLLGRLLASKENERIADVEQMHLDMIPLKDYTEAQELDRRLGEDVTLKNALVHHLSLLGGQDLNKIVKGILKELMTKDLSQQCNFKGHRGPKKAFSVLANILNVTLSKFLKKVLQRYFLLPSVSVHKTGSKFCSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CT44</accession>
    <name>A0A9Q1CT44_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">HOLleu_04561</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">Nanhai2018</strain>
            <tissue evidence="2">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8051112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CT44"/>
    <dbReference type="OrthoDB" id="10143009at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="130"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="227"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="347"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="167"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="189"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="229"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAJ8051112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="367" mass="41176" checksum="4781CB683E7E90C6" modified="2023-09-13" version="1">MENSPRISRASPEVEDLEDEDVNVYTGDDEEIVMRERSASDMTKLLNVPRLGESLKYPSAPDVASDASDDQRRSVLAMTAMDGSESSDVVPVRNLDYRLKERHVYRETLDKETRLGVYCSPRFYRKKTFGASGSNSYGEGSFREDFGMDNSMDNSPTDSNGSSARSELSRKSYQENIRKKRPLSLVGLDTRRRFREGQNGKHTEGITNLGSSCCNLRSSLVERDYRLRSPTSRGDHQSSLSPFTERSPSFQLSRSCSDLRDGMTVNVSPMHPTIQELRCPSHVSLQSIRVSSPSESRISLLSDLEIYERAHHRPRHADSPPLYPDVGCPALSSTALVQEWLARQEKYSLSKHNSDSGGKPRTGSLPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CTN9</accession>
    <name>A0A9Q1CTN9_HOLLE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein NLRC5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">HOLleu_03972</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">Nanhai2018</strain>
            <tissue evidence="3">Muscle</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CTN9"/>
    <dbReference type="OrthoDB" id="427518at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312">
        <property type="entry name" value="NACHT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46312:SF2">
        <property type="entry name" value="NUCLEOTIDE-BINDING OLIGOMERIZATION DOMAIN-CONTAINING PROTEIN 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="NACHT" evidence="2">
        <location>
            <begin position="229"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAJ8050675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001152320"/>
        </source>
    </evidence>
    <sequence length="453" mass="51700" checksum="E0508E497D0C3DF6" modified="2023-09-13" version="1">MLARRETMDTNEELEFDTATGVIGGFGRFLVAVVALLTVATLRKLSNVFNYPPSLIEEFENDRNPGYVFVMSLRERGIILPNDVSVLIDALRDIGLHGIAYEVTRAFEKHCKQKETLESQNKVRERGHRPSTDEVIDSFRKQLSVSQSTDASPNQRSQKATFVNSIKITYRGIYEKVQPIPFIRDNLLCVDNVFIDSGVERLIKKGVNGTWQKLESYHCLFNDLPPAPTRCILFGEPGFGKSTLALQLVYDWCNSCLNSPLKDVDILIFLRLRQLMDVSSIFKAIKQFILPHDSPLSESDITEIIYSSKSVVILLDGFDEYANKRGADDDDVTKIITGRMFQNLFVVVTTRPSFKPVEMPANTVQLRLKGFDTEAQENYLRKTVGGSDLKAITKIRSYIEENHILRDLCEVPLFFAMFSHMFFQKEMTMQFDSATSFFRYMIGSFHEHMKNKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9Q1CTR2</accession>
    <name>A0A9Q1CTR2_HOLLE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">UDP-D-xylose:beta-D-glucoside alpha-1,3-D-xylosyltransferase</fullName>
            <ecNumber evidence="11">2.4.2.42</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">HOLleu_04028</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Holothuria leucospilota</name>
        <name type="common">Black long sea cucumber</name>
        <name type="synonym">Mertensiothuria leucospilota</name>
        <dbReference type="NCBI Taxonomy" id="206669"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Echinodermata</taxon>
            <taxon>Eleutherozoa</taxon>
            <taxon>Echinozoa</taxon>
            <taxon>Holothuroidea</taxon>
            <taxon>Aspidochirotacea</taxon>
            <taxon>Aspidochirotida</taxon>
            <taxon>Holothuriidae</taxon>
            <taxon>Holothuria</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2021-10" db="EMBL/GenBank/DDBJ databases">
            <title>Tropical sea cucumber genome reveals ecological adaptation and Cuvierian tubules defense mechanism.</title>
            <authorList>
                <person name="Chen T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">Nanhai2018</strain>
            <tissue evidence="15">Muscle</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Glycosyltransferase which elongates the O-linked glucose attached to EGF-like repeats in the extracellular domain of Notch proteins by catalyzing the addition of xylose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>3-O-(beta-D-glucosyl)-L-seryl-[EGF-like domain protein] + UDP-alpha-D-xylose = 3-O-[alpha-D-xylosyl-(1-&gt;3)-beta-D-glucosyl]-L-seryl-[EGF-like domain protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:56064"/>
            <dbReference type="Rhea" id="RHEA-COMP:14610"/>
            <dbReference type="Rhea" id="RHEA-COMP:14611"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57632"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:140575"/>
            <dbReference type="ChEBI" id="CHEBI:140576"/>
            <dbReference type="EC" id="2.4.2.42"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.42" evidence="11"/>
    <dbReference type="EMBL" id="JAIZAY010000001">
        <property type="protein sequence ID" value="KAJ8050720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9Q1CTR2"/>
    <dbReference type="OrthoDB" id="6238971at2759"/>
    <dbReference type="Proteomes" id="UP001152320">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140563">
        <property type="term" value="F:UDP-D-xylose:beta-D-glucoside alpha-1,3-D-xylosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016266">
        <property type="term" value="P:protein O-linked glycosylation via N-acetyl-galactosamine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002495">
        <property type="entry name" value="Glyco_trans_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051993">
        <property type="entry name" value="Glycosyltransferase_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46012">
        <property type="entry name" value="IP22168P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46012:SF2">
        <property type="entry name" value="IP22168P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01501">
        <property type="entry name" value="Glyco_transf_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="73"/>
            <end position="114"/>
 